                         SEQUENCE LISTING

<110>   Eastern Washington University
       Washington State University
 
<120>  BACTERIA PRODUCING GAMMA-AMINOBUTYRIC ACID (GABA) AND USES OF THE
        BACTERIA

<130>  E133-0002PCT

<150>  US 63/141,837
<151>  2021-01-26

<160>  27    

<170>  PatentIn version 3.5

<210>  1
<211>  466
<212>  PRT
<213>  Lactococcus lactis

<400>  1

Met Leu Tyr Gly Lys Glu Asn Arg Asp Glu Ala Glu Phe Leu Glu Pro 
1               5                   10                  15      


Ile Phe Gly Ser Glu Ser Glu Gln Val Asp Leu Pro Lys Tyr Lys Leu 
            20                  25                  30          


Ala Gln Gln Ser Ile Glu Pro Arg Val Ala Tyr Gln Leu Val Gln Asp 
        35                  40                  45              


Glu Met Leu Asp Glu Gly Asn Ala Arg Leu Asn Leu Ala Thr Phe Cys 
    50                  55                  60                  


Gln Thr Tyr Met Glu Pro Glu Ala Val Lys Leu Met Ser Gln Thr Leu 
65                  70                  75                  80  


Glu Lys Asn Ala Ile Asp Lys Ser Glu Tyr Pro Arg Thr Thr Glu Ile 
                85                  90                  95      


Glu Asn Arg Cys Val Asn Met Ile Ala Asp Leu Trp Asn Ala Ser Glu 
            100                 105                 110         


Lys Glu Lys Phe Met Gly Thr Ser Thr Ile Gly Ser Ser Glu Ala Cys 
        115                 120                 125             


Met Leu Gly Gly Met Ala Met Lys Phe Ser Trp Arg Lys Arg Ala Glu 
    130                 135                 140                 


Lys Leu Gly Leu Asp Ile Asn Ala Lys Lys Pro Asn Leu Val Ile Ser 
145                 150                 155                 160 


Ser Gly Tyr Gln Val Cys Trp Glu Lys Phe Cys Ile Tyr Trp Asp Ile 
                165                 170                 175     


Glu Met Arg Glu Val Pro Met Asp Lys Glu His Met Ser Ile Asn Leu 
            180                 185                 190         


Asp Lys Val Met Asp Tyr Val Asp Glu Tyr Thr Ile Gly Val Val Gly 
        195                 200                 205             


Ile Met Gly Ile Thr Tyr Thr Gly Arg Tyr Asp Asp Ile Lys Ala Leu 
    210                 215                 220                 


Asp Asn Leu Ile Glu Glu Tyr Asn Lys Gln Thr Asp Tyr Lys Val Tyr 
225                 230                 235                 240 


Ile His Val Asp Ala Ala Ser Gly Gly Leu Tyr Ala Pro Phe Val Glu 
                245                 250                 255     


Pro Glu Leu Glu Trp Asp Phe Arg Leu Lys Asn Val Ile Ser Ile Asn 
            260                 265                 270         


Thr Ser Gly His Lys Tyr Gly Leu Val Tyr Pro Gly Val Gly Trp Val 
        275                 280                 285             


Leu Trp Arg Asp Lys Lys Tyr Leu Pro Glu Glu Leu Ile Phe Lys Val 
    290                 295                 300                 


Ser Tyr Leu Gly Gly Glu Leu Pro Thr Met Ala Ile Asn Phe Ser His 
305                 310                 315                 320 


Ser Ala Ser Gln Leu Ile Gly Gln Tyr Tyr Asn Phe Val Arg Tyr Gly 
                325                 330                 335     


Phe Asp Gly Tyr Lys Ala Ile His Glu Arg Thr His Lys Val Ala Met 
            340                 345                 350         


Phe Leu Ala Lys Glu Ile Glu Lys Thr Gly Met Phe Glu Ile Met Asn 
        355                 360                 365             


Asp Gly Ser Gln Leu Pro Ile Val Cys Tyr Lys Leu Lys Glu Asp Ser 
    370                 375                 380                 


Asn Arg Gly Trp Asn Leu Tyr Asp Leu Ala Asp Arg Leu Leu Met Lys 
385                 390                 395                 400 


Gly Trp Gln Val Pro Ala Tyr Pro Leu Pro Lys Asn Leu Glu Asn Glu 
                405                 410                 415     


Ile Ile Gln Arg Leu Val Ile Arg Ala Asp Phe Gly Met Asn Met Ala 
            420                 425                 430         


Phe Asn Tyr Val Gln Asp Met Gln Glu Ala Ile Glu Ala Leu Asn Lys 
        435                 440                 445             


Ala His Ile Leu Tyr His Glu Glu Pro Glu Asn Lys Thr Tyr Gly Phe 
    450                 455                 460                 


Thr His 
465     


<210>  2
<211>  503
<212>  PRT
<213>  Lactococcus lactis

<400>  2

Met Asn Gln Lys Lys Ile Ser Leu Phe Gly Phe Phe Ala Leu Thr Ala 
1               5                   10                  15      


Ser Met Val Leu Thr Val Tyr Glu Tyr Pro Thr Phe Ala Thr Ser Lys 
            20                  25                  30          


Leu His Leu Val Phe Phe Leu Leu Leu Gly Gly Leu Leu Trp Phe Leu 
        35                  40                  45              


Pro Val Ala Leu Cys Ala Ala Glu Met Ala Thr Val Glu Gly Trp Lys 
    50                  55                  60                  


Asn Gly Gly Ile Phe Ser Trp Val Ser Gln Thr Leu Gly Glu Arg Phe 
65                  70                  75                  80  


Gly Phe Ala Ala Ile Phe Phe Gln Trp Phe Gln Ile Thr Val Gly Phe 
                85                  90                  95      


Val Thr Met Ile Tyr Phe Ile Leu Gly Ala Leu Ser Tyr Val Leu Asn 
            100                 105                 110         


Phe Gln Ala Leu Asn Thr Asp Pro Leu Ile Lys Phe Ile Gly Leu Leu 
        115                 120                 125             


Ile Ile Phe Trp Gly Leu Thr Phe Ser Gln Leu Gly Gly Thr Gln Arg 
    130                 135                 140                 


Thr Ala Lys Leu Val Lys Ala Gly Phe Val Val Gly Ile Val Ile Pro 
145                 150                 155                 160 


Ser Ile Ile Leu Phe Gly Leu Ala Ala Ala Tyr Phe Ile Gly Gly Asn 
                165                 170                 175     


Pro Ile Glu Ile Pro Ile Asn Lys His Ala Phe Val Pro Asp Phe Ser 
            180                 185                 190         


Gln Val Ser Thr Leu Val Val Phe Val Ser Phe Ile Leu Ala Tyr Met 
        195                 200                 205             


Gly Val Glu Ala Ser Ala Ser His Ile Asn Glu Leu Glu Asn Pro Lys 
    210                 215                 220                 


Arg Asn Tyr Pro Leu Ala Met Ile Leu Leu Val Ile Leu Ala Ile Ser 
225                 230                 235                 240 


Leu Asp Ala Ile Gly Gly Phe Ser Val Ala Ala Val Ile Pro Gln Lys 
                245                 250                 255     


Asp Leu Ser Leu Ser Ala Gly Val Ile Gln Thr Phe Gln Thr Leu Ile 
            260                 265                 270         


Leu His Phe Asn His His Leu Gly Trp Leu Val Lys Val Ile Ala Leu 
        275                 280                 285             


Met Ile Ala Phe Gly Val Met Gly Glu Val Ser Ser Trp Val Val Gly 
    290                 295                 300                 


Pro Ser Arg Gly Met Phe Ala Ala Ala Gln Arg Gly Leu Leu Pro Lys 
305                 310                 315                 320 


Phe Leu Arg Lys Thr Asn Thr His Glu Val Pro Val Pro Leu Val Met 
                325                 330                 335     


Ile Gln Gly Ile Ile Val Thr Leu Trp Gly Ala Val Leu Thr Phe Gly 
            340                 345                 350         


Gly Gly Gly Asn Asn Leu Ser Phe Leu Val Ala Ile Ser Leu Thr Val 
        355                 360                 365             


Val Ile Tyr Leu Val Gly Tyr Leu Leu Phe Phe Ile Gly Tyr Phe Val 
    370                 375                 380                 


Leu Ile Tyr Lys Lys Gln Asn Leu Lys Arg Thr Tyr Asn Val Pro Gly 
385                 390                 395                 400 


Lys Arg Val Gly Lys Thr Ile Ile Ala Gly Ile Gly Phe Leu Leu Ser 
                405                 410                 415     


Ile Phe Ala Leu Phe Ile Ser Phe Val Pro Pro Ala Ser Ile Ala Lys 
            420                 425                 430         


Asn Glu Thr His Thr Tyr Gln Met Ile Leu Leu Ile Ser Phe Val Val 
        435                 440                 445             


Thr Ala Ile Leu Pro Phe Ile Val Tyr Glu Leu His Asp Lys Arg Gly 
    450                 455                 460                 


His Asp Thr Ile Glu Glu Pro Arg His Phe Lys Ala Arg Asp Val Asn 
465                 470                 475                 480 


Pro Ala Ile Tyr Pro Ala Ala Arg Gly Glu His His Ile Ile Lys Lys 
                485                 490                 495     


Glu Glu His Ile Leu Lys His 
            500             


<210>  3
<211>  1401
<212>  DNA
<213>  Lactococcus lactis

<400>  3
atgttatacg gaaaagaaaa tcgcgatgaa gcagagttct tggaaccaat ttttggttca       60

gaaagtgaac aagtggattt gcctaaatat aaattagctc aacaatcaat tgaacctcga      120

gtggcctatc agttagttca agatgaaatg ttagatgaag ggaacgctcg tttaaattta      180

gccacattct gtcaaactta tatggaacct gaagcagtca aactaatgag tcaaaccttg      240

gaaaaaaatg caattgataa atcggaatat ccaagaacaa ctgaaattga gaaccgttgc      300

gtcaacatga tcgctgacct ttggaatgcg agtgaaaaag aaaaatttat ggggacttca      360

acgattggtt cttcagaagc ttgtatgctt ggtggaatgg ccatgaagtt ttcttggcgc      420

aagcgagcag aaaaattggg attagatatt aatgcgaaaa agccaaactt agttatttca      480

tctggttatc aagtttgctg ggaaaaattc tgtatttatt gggatattga aatgcgagaa      540

gtgccaatgg ataaagaaca tatgtcaatc aatttggaca aggtgatgga ttatgttgat      600

gagtacacga ttggtgtagt tggtattatg gggattactt atactggtcg ttatgatgat      660

atcaaagctt tggataattt aattgaagaa tataataaac agacagacta taaagtttat      720

attcacgtag atgctgcttc aggaggactt tatgctccct ttgttgagcc agaacttgag      780

tgggatttcc gtttgaaaaa tgtcatttca atcaatacct caggccataa atatggttta      840

gtttatcctg gtgtaggttg ggttttgtgg cgtgacaaaa aatatttacc agaagaatta      900

atttttaaag taagttatct tggaggagaa ctaccaacga tggccattaa tttttctcat      960

agtgcctctc aattaattgg tcaatattat aattttgtac gttatggatt tgatggatat     1020

aaagctattc atgagagaac acataaagta gccatgtttt tagcaaaaga aattgaaaaa     1080

actggaatgt ttgaaattat gaacgatggg tcacaattgc caattgtctg ctataaatta     1140

aaagaagatt caaatcgagg ttggaatctt tatgatttgg cggaccgttt attaatgaag     1200

ggatggcaag tgcctgctta tccacttccc aaaaatttgg aaaatgaaat cattcaacgt     1260

ttagtgattc gagcagattt tgggatgaat atggcattta actatgttca agatatgcaa     1320

gaagcaattg aggctttaaa taaggctcat attctatatc atgaagagcc tgaaaataaa     1380

acatatggat ttactcacta a                                               1401


<210>  4
<211>  1512
<212>  DNA
<213>  Lactococcus lactis

<400>  4
atgaatcaaa aaaaaatatc attattcggt ttttttgcat taaccgcttc aatggttttg       60

actgtctatg agtatccaac ttttgccacg tcaaaattac atttggtgtt ctttttactt      120

ctcggaggac tactctggtt tttgcccgta gcgctctgtg cagcagaaat ggcgacggtt      180

gaaggttgga aaaatggtgg aatttttagt tgggtgagcc aaactttagg ggagcgcttt      240

ggttttgcag ccatattttt tcagtggttt caaattacag ttggctttgt cactatgatc      300

tatttcattt taggggccct ctcttatgtg ttaaattttc aggcacttaa tacagatcca      360

ttgattaaat ttattggttt actaatcatt ttttggggat tgacattttc tcagttaggt      420

gggacacaaa gaactgctaa attggtaaaa gctggttttg tagttgggat agtgattcca      480

tcgattatct tgtttggatt agctgcggca tattttatcg gaggtaatcc tatagagata      540

ccaattaata agcatgcttt tgtaccagat ttttcacaag tatcaacttt agtagttttt      600

gtttctttta ttcttgctta tatgggggta gaagcttcag cttcgcacat taatgaactt      660

gaaaatccaa agagaaatta tcccttagca atgattttat tagtaatatt agctatttct      720

ttagatgcca taggtggatt ttctgtagca gcggttattc ctcaaaaaga cttatcatta      780

agtgcagggg taattcaaac ttttcaaacg ttaatcttac attttaatca tcatttggga      840

tggttagtta aagtaattgc actaatgatt gcctttgggg ttatgggaga agtgagttca      900

tgggttgttg gtccttctag agggatgttt gcagcagcac aaagaggctt attaccaaaa      960

tttttacgta aaacaaatac gcatgaagtc cctgttcctt tagttatgat tcaaggaatc     1020

attgttacac tttggggcgc tgtattaact tttggaggag gaggaaataa tttatctttc     1080

ttagttgcca tttcactgac agtagtgatt tatttggtag gttatctctt attctttatt     1140

ggttactttg ttttaatcta taaaaaacaa aatttaaagc gcacttataa tgttccaggt     1200

aaaagagttg gaaaaacaat cattgcggga attggatttt tattatcaat ttttgctcta     1260

tttatttcct ttgttcctcc agcatcaatt gcgaaaaatg agactcatac ttaccaaatg     1320

atacttctta taagttttgt tgtgactgct atcttgccat ttattgttta tgaattgcat     1380

gataaaaggg gacatgatac tattgaagaa ccaaggcact ttaaagcaag agatgtgaac     1440

cccgcgattt atccagcagc tcgtggtgag catcatatta ttaaaaaaga agaacatatc     1500

ttaaaacatt aa                                                         1512


<210>  5
<211>  151
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized P8 promoter (short)

<400>  5
tttttttagg acaattattt ctcataaaaa gcagatttta gaaagaaaat tgtatttttt       60

taacagcttt gactgccctt tttggaagag tttatgtata atagaattag ttagttttgc      120

tattgatata gcagcagaaa tggagagata t                                     151


<210>  6
<211>  169
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized P8 promoter

<400>  6
gataaaattt ctaatgattt ttttaggaca attatttctc ataaaaagca gattttagaa       60

agaaaattgt atttttttaa cagctttgac tgcccttttt ggaagagttt atgtataata      120

gaattagtta gttttgctat tgatatagca gcagaaatgg agagatata                  169


<210>  7
<211>  513
<212>  PRT
<213>  Lactococcus lactis

<400>  7

Met Arg Lys Ile Glu Phe Phe Asp Thr Ser Leu Arg Asp Gly Glu Gln 
1               5                   10                  15      


Thr Pro Gly Val Ser Phe Ser Ile Ser Glu Lys Val Thr Ile Ala Lys 
            20                  25                  30          


Gln Leu Glu Lys Trp Arg Ile Ser Val Ile Glu Ala Gly Phe Ser Ala 
        35                  40                  45              


Ala Ser Pro Asp Ser Phe Glu Ala Val Lys Gln Ile Ala Asp Ser Leu 
    50                  55                  60                  


Asn Asp Thr Ala Val Thr Ala Leu Ala Arg Cys Val Ile Ser Asp Ile 
65                  70                  75                  80  


Asp Lys Ala Val Glu Ala Val Lys Gly Ala Lys Tyr Pro Gln Ile His 
                85                  90                  95      


Val Phe Ile Ala Thr Ser Pro Ile His Met Lys Tyr Lys Leu Lys Ile 
            100                 105                 110         


Ser Pro Glu Glu Val Leu Lys Asn Ile Asp Lys Cys Val Arg Tyr Ala 
        115                 120                 125             


Arg Glu Arg Val Glu Val Val Glu Phe Ser Pro Glu Asp Ala Thr Arg 
    130                 135                 140                 


Thr Glu Leu Asn Phe Leu Leu Glu Ala Val Gln Thr Ala Val Asp Ala 
145                 150                 155                 160 


Gly Ala Thr Tyr Ile Asn Ile Pro Asp Thr Val Gly Tyr Thr Thr Pro 
                165                 170                 175     


Glu Glu Tyr Gly Lys Ile Phe Lys Phe Leu Ile Asp Asn Thr Lys Ser 
            180                 185                 190         


Asp Arg Glu Ile Ile Phe Ser Pro His Cys His Asp Asp Leu Gly Met 
        195                 200                 205             


Ala Val Ala Asn Ser Leu Ala Ala Ile Lys Ala Gly Ala Gly Arg Val 
    210                 215                 220                 


Glu Gly Thr Val Asn Gly Ile Gly Glu Arg Ala Gly Asn Ala Ala Leu 
225                 230                 235                 240 


Glu Glu Ile Ala Val Ala Leu His Ile Arg Lys Asp Phe Tyr Gln Ala 
                245                 250                 255     


Gln Ser Pro Leu Lys Leu Ser Glu Thr Ala Ala Thr Ala Glu Leu Ile 
            260                 265                 270         


Ser Gln Phe Ser Gly Ile Ala Ile Pro Lys Asn Lys Ala Ile Val Gly 
        275                 280                 285             


Ala Asn Ala Phe Ala His Glu Ser Gly Ile His Gln Asp Gly Val Leu 
    290                 295                 300                 


Lys Asn Ala Glu Thr Tyr Glu Ile Ile Thr Pro Glu Leu Val Gly Ile 
305                 310                 315                 320 


Lys His Asn Ser Leu Pro Leu Gly Lys Leu Ser Gly Arg His Ala Phe 
                325                 330                 335     


Ser Glu Lys Leu Thr Glu Leu Asn Ile Ala Tyr Asp Asp Glu Ser Leu 
            340                 345                 350         


Ala Ile Leu Phe Glu Lys Phe Lys Lys Leu Ala Asp Lys Lys Lys Glu 
        355                 360                 365             


Ile Thr Asp Ala Asp Ile His Ala Leu Phe Thr Gly Glu Thr Val Lys 
    370                 375                 380                 


Asn Leu Ala Gly Phe Ile Leu Asp Asn Val Gln Ile Asp Gly His Lys 
385                 390                 395                 400 


Ala Leu Val Gln Leu Lys Asn Gln Glu Glu Glu Ile Tyr Val Ser Gln 
                405                 410                 415     


Gly Glu Gly Ser Gly Ser Val Asp Ala Ile Phe Lys Ala Ile Asp Lys 
            420                 425                 430         


Val Phe Asn His Gln Leu Lys Leu Ile Ser Tyr Ser Val Asp Ala Val 
        435                 440                 445             


Thr Asp Gly Ile Asp Ala Gln Ala Thr Thr Leu Val Ser Val Glu Asn 
    450                 455                 460                 


Leu Ser Thr Gly Thr Ile Phe Asn Ala Lys Gly Val Asp Tyr Asp Val 
465                 470                 475                 480 


Leu Lys Gly Ser Ala Ile Ala Tyr Met Asn Ala Asn Val Leu Val Gln 
                485                 490                 495     


Lys Glu Asn Leu Gln Gly Lys Val Glu Gln Ile Ser Ala His Asp Gly 
            500                 505                 510         


Ile 
    


<210>  8
<211>  1542
<212>  DNA
<213>  Lactococcus lactis

<400>  8
atgcgaaaaa ttgaattctt tgacacaagt ttgagagatg gcgaacagac accgggcgtt       60

agtttctcca tttcagaaaa agtaacgatt gctaaacaac tggaaaaatg gaggatttct      120

gtcatagagg ctggtttttc tgcggcaagt ccagatagtt ttgaagcagt aaagcaaatt      180

gctgattctt tgaatgatac ggctgtcact gcattagctc gctgtgttat ttcagatatc      240

gataaagcgg ttgaagcggt aaagggggct aaatatccgc aaattcatgt tttcattgca      300

acttcaccta ttcacatgaa atataaactt aaaatcagtc ccgaagaagt tttgaaaaat      360

attgataagt gtgtgagata cgcacgtgaa cgggtcgagg ttgttgagtt ttctccagag      420

gatgcaacaa gaacggagtt gaattttctt ttagaggctg ttcaaacggc tgtcgatgct      480

ggagcaactt atattaatat tcctgacact gtcggttata cgacaccaga agaatatgga      540

aaaattttta aatttttgat tgataatact aagtctgacc gagaaattat ttttagtcca      600

cattgtcatg atgatttagg aatggctgta gctaattcat tagctgcaat taaagctggg      660

gctgggagag ttgaaggaac tgtcaatggt attggagagc gagctgggaa tgctgctctt      720

gaagaaattg ctgtggcact acatattcgt aaagattttt atcaggcaca aagtccttta      780

aaactttcag aaactgctgc aacggcagaa ctaatttcac aattttcagg aattgctatt      840

ccaaaaaata aagcaattgt tggtgctaat gcttttgcac acgaatcagg aattcatcaa      900

gatggtgtcc ttaaaaatgc tgaaacttat gaaattatta caccagaact tgtcggaata      960

aagcataatt cgttgccttt aggtaaactt tctggtcgtc atgcttttag tgaaaaattg     1020

acggaactta atattgctta tgacgatgaa agtcttgcaa ttttatttga aaaatttaaa     1080

aaattagctg acaagaaaaa agaaattact gacgcagata ttcatgcctt gtttacagga     1140

gaaacggtaa aaaatctagc tggatttata cttgataatg ttcaaattga tgggcacaag     1200

gcattggtgc aactaaaaaa tcaagaagag gaaatttatg ttagccaagg agaggggtca     1260

ggttcagtgg atgcaatttt taaagctatt gataaagtct ttaatcatca actaaaatta     1320

atttcctatt cagttgatgc tgtaactgat ggaattgatg cacaagcaac gactttggtt     1380

tctgttgaaa atctatctac aggcactata tttaatgcta aaggtgttga ttatgatgta     1440

ttgaaaggaa gcgccattgc ttacatgaac gctaatgttt tagttcaaaa agaaaattta     1500

caaggaaagg ttgaacaaat ttcagctcat gatggaattt aa                        1542


<210>  9
<211>  369
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized upstream leuA homology arm

<400>  9
caagtttgag agatggcgaa cagacgccgg gcgttagttt ctgcatttca gaaaaagtaa       60

cgattgctaa acaactggaa aaatggggga tttctgtcat agaggctggt tttcctgcgg      120

caagtccaga tagttttgaa gcagtaaagc aaattgctga ttctttgaat gatacggctg      180

tcactgcatt agctcgttgt gttatctcag atatcgataa agcggttgaa gcggtaaagg      240

gggctaaata tccgcaaatt catgttttca ttgccacttc acctattcac atgaaatata      300

aacttaaaat cagtcccgaa gaagttttga aaaatattga taaatgtgtg aaatacgcac      360

gtgaacggg                                                              369


<210>  10
<211>  262
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized downstream leuA homology arm

<400>  10
gaataggggg caggttcact ggatgcaatt tttaaagcta ttgataaagt ctttaatcat       60

caactaaaat taatttccta ttcagttgat gctgtaactg atggaattga tgcacaagca      120

acgactttgg tttctgttga aaatctatct acaggcacta tatttaatgc taaaggtgtt      180

gattatgatg tattgaaagg aagcgccatt gcttacatga acgctaatgt tttagttcaa      240

aaagaaaatt tacaaggaaa gg                                               262


<210>  11
<211>  3933
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized genetic construct including upstream leuA homology 
       arm, P8 short promoter, gadC gene, gadB gene, and downstream leuA
       homology arm

<400>  11
ctgcagccat catgagctga aatttgttca acctttcctt gtaaattttc tttttgaact       60

aaaacattag cgttcatgta agcaatggcg cttcctttca atacatcata atcaacacct      120

ttagcattaa atatagtgcc tgtagataga ttttcaacag aaaccaaagt cgttgcttgt      180

gcatcaattc catcagttac agcatcaact gaataggaaa ttaattttag ttgatgatta      240

aagactttat caatagcttt aaaaattgca tccagtgaac ctgcccccta ttcttttttt      300

aggacaatta tttctcataa aaagcagatt ttagaaagaa aattgtattt ttttaacagc      360

tttgactgcc ctttttggaa gagtttatgt ataatagaat tagttagttt tgctattgat      420

atagcagcag aaatggagag atatgatgaa tcaaaaaaaa atatcattat tcggtttttt      480

tgcattaacc gcttcaatgg ttttgactgt ctatgagtat ccaacttttg ccacgtcaaa      540

attacatttg gtgttctttt tacttctcgg aggactactc tggtttttgc ccgtagcgct      600

ctgtgcagca gaaatggcga cggttgaagg ttggaaaaat ggtggaattt ttagttgggt      660

gagccaaact ttaggggagc gctttggttt tgcagccata ttttttcagt ggtttcaaat      720

tacagttggc tttgtcacta tgatctattt cattttaggg gccctctctt atgtgttaaa      780

ttttcaggca cttaatacag atccattgat taaatttatt ggtttactaa tcattttttg      840

gggattgaca ttttctcagt taggtgggac acaaagaact gctaaattgg taaaagctgg      900

ttttgtagtt gggatagtga ttccatcgat tatcttgttt ggattagctg cggcatattt      960

tatcggaggt aatcctatag agataccaat taataagcat gcttttgtac cagatttttc     1020

acaagtatca actttagtag tttttgtttc ttttattctt gcttatatgg gggtagaagc     1080

ttcagcttcg cacattaatg aacttgaaaa tccaaagaga aattatccct tagcaatgat     1140

tttattagta atattagcta tttctttaga tgccataggt ggattttctg tagcagcggt     1200

tattcctcaa aaagacttat cattaagtgc aggggtaatt caaacttttc aaacgttaat     1260

cttacatttt aatcatcatt tgggatggtt agttaaagta attgcactaa tgattgcctt     1320

tggggttatg ggagaagtga gttcatgggt tgttggtcct tctagaggga tgtttgcagc     1380

agcacaaaga ggcttattac caaaattttt acgtaaaaca aatacgcatg aagtccctgt     1440

tcctttagtt atgattcaag gaatcattgt tacactttgg ggcgctgtat taacttttgg     1500

aggaggagga aataatttat ctttcttagt tgccatttca ctgacagtag tgatttattt     1560

ggtaggttat ctcttattct ttattggtta ctttgtttta atctataaaa aacaaaattt     1620

aaagcgcact tataatgttc caggtaaaag agttggaaaa acaatcattg cgggaattgg     1680

atttttatta tcaatttttg ctctatttat ttcctttgtt cctccagcat caattgcgaa     1740

aaatgagact catacttacc aaatgatact tcttataagt tttgttgtga ctgctatctt     1800

gccatttatt gtttatgaat tgcatgataa aaggggacat gatactattg aagaaccaag     1860

gcactttaaa gcaagagatg tgaaccccgc gatttatcca gcagctcgtg gtgagcatca     1920

tattattaaa aaagaagaac atatcttaaa acattaaaaa attggaggat gtacctatgt     1980

tatacggaaa agaaaatcgc gatgaagcag agttcttgga accaattttt ggttcagaaa     2040

gtgaacaagt ggatttgcct aaatataaat tagctcaaca atcaattgaa cctcgagtgg     2100

cctatcagtt agttcaagat gaaatgttag atgaagggaa cgctcgttta aatttagcca     2160

cattctgtca aacttatatg gaacctgaag cagtcaaact aatgagtcaa accttggaaa     2220

aaaatgcaat tgataaatcg gaatatccaa gaacaactga aattgagaac cgttgcgtca     2280

acatgatcgc tgacctttgg aatgcgagtg aaaaagaaaa atttatgggg acttcaacga     2340

ttggttcttc agaagcttgt atgcttggtg gaatggccat gaagttttct tggcgcaagc     2400

gagcagaaaa attgggatta gatattaatg cgaaaaagcc aaacttagtt atttcatctg     2460

gttatcaagt ttgctgggaa aaattctgta tttattggga tattgaaatg cgagaagtgc     2520

caatggataa agaacatatg tcaatcaatt tggacaaggt gatggattat gttgatgagt     2580

acacgattgg tgtagttggt attatgggga ttacttatac tggtcgttat gatgatatca     2640

aagctttgga taatttaatt gaagaatata ataaacagac agactataaa gtttatattc     2700

acgtagatgc tgcttcagga ggactttatg ctccctttgt tgagccagaa cttgagtggg     2760

atttccgttt gaaaaatgtc atttcaatca atacctcagg ccataaatat ggtttagttt     2820

atcctggtgt aggttgggtt ttgtggcgtg acaaaaaata tttaccagaa gaattaattt     2880

ttaaagtaag ttatcttgga ggagaactac caacgatggc cattaatttt tctcatagtg     2940

cctctcaatt aattggtcaa tattataatt ttgtacgtta tggatttgat ggatataaag     3000

ctattcatga gagaacacat aaagtagcca tgtttttagc aaaagaaatt gaaaaaactg     3060

gaatgtttga aattatgaac gatgggtcac aattgccaat tgtctgctat aaattaaaag     3120

aagattcaaa tcgaggttgg aatctttatg atttggcgga ccgtttatta atgaagggat     3180

ggcaagtgcc tgcttatcca cttcccaaaa atttggaaaa tgaaatcatt caacgtttag     3240

tgattcgagc agattttggg atgaatatgg catttaacta tgttcaagat atgcaagaag     3300

caattgaggc tttaaataag gctcatattc tatatcatga agagcctgaa aataaaacat     3360

atggatttac tcactaaaaa aaccgatatt catcttaaga aaaatcaaaa gcgatatagt     3420

taatctatat cgcttttatt ttgatgataa attttgtttt tttctattta gtcataagtt     3480

tgtcagtgat tatttttcat aatatttatc taaaatctcc atttttaaag gagtaatctt     3540

agataatggg gctgcccgtt cacgtgcgta tttcacacat ttatcaatat ttttcaaaac     3600

ttcttcggga ctgattttaa gtttatattt catgtgaata ggtgaagtgg caatgaaaac     3660

atgaatttgc ggatatttag ccccctttac cgcttcaacc gctttatcga tatctgagat     3720

aacacaacga gctaatgcag tgacagccgt atcattcaaa gaatcagcaa tttgctttac     3780

tgcttcaaaa ctatctggac ttgccgcagg aaaaccagcc tctatgacag aaatccccca     3840

tttttccagt tgtttagcaa tcgttacttt ttctgaaatg cagaaactaa cgcccggcgt     3900

ctgttcgcca tctctcaaac ttgtgtcctg cag                                  3933


<210>  12
<211>  230
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized P2 promoter

<400>  12
tttctcctat cttttctatt tgacatctaa atccattata aggcaaagtg tattaaaaag       60

acagcttcac tatgattagt acacaaataa tcatttaaaa agtgtaaata attttttata      120

aataacgaat caaaaagttt gaccattact gaccaaagta ttataattag aatgtagtga      180

gaaaaagata ataattacta ctaactatga aatcgaaatg aggtgttttt                 230


<210>  13
<211>  210
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized P5 promoter

<400>  13
gaaaaagaaa atgtttttgt atttttagaa tcccttttct ataaatcaat tctaattata       60

aggacctgat gattgagtga taatgctagt ttgaagcatt cttagtaaga aagtgatttt      120

ttataaatgg tttatagaat aaattgtaca gcgtttaatt ggacttgctc tctgaaataa      180

cgtaaaattg tagtgaggag gacggttaca                                       210


<210>  14
<211>  300
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P2 with tails promoter clone sequence

<400>  14
agatctgcat ccagtgaacc tgccccctat tctttctcct atcttttcta ttttgacatc       60

taaatccatt ataaggcaaa gtgtattaaa aagacagctt cactatgatt agtacacaaa      120

taattcattt aaaaagtgta aataattttt tataaataac gaatcaaaaa gtttgaccat      180

tactgaccaa agtattataa ttagaatgta gtgagaaaaa gataataatt actactaact      240

atgaaatcga aatgaggtgt ttttgatgaa tcaaaaaaaa atatcattat tcggagatct      300


<210>  15
<211>  278
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P5 with tails promoter clone sequence

<400>  15
agatctgcat ccagtgaacc tgccccctat tcgaaaaaga aaatgttttt gtatttttag       60

aatccctttt ctataaatca attctaatta taaggacctg atgattgagt gataatgcta      120

gtttgaagca ttcttagtaa gaaagtgatt ttttataaat ggtttataga ataaattgta      180

cagcgtttaa ttggacttgc tctctgaaat aacgtaaaat tgtagtgagg aggacggtta      240

cagatgaatc aaaaaaaaat atcattattc ggagatct                              278


<210>  16
<211>  219
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P8s with tails promoter clone sequence

<400>  16
agatctgcat ccagtgaacc tgccccctat tcttttttta ggacaattat ttctcataaa       60

aagcagattt tagaaagaaa attgtatttt tttaacagct ttgactgccc tttttggaag      120

agtttatgta taatagaatt agttagtttt gctattgata tagcagcaga aatggagaga      180

tatgatgaat caaaaaaaaa tatcattatt cggagatct                             219


<210>  17
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer for leuA upstream

<400>  17
cgaggaccga gagacgtcct cacg                                              24


<210>  18
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer for leuA downstream

<400>  18
gcccaattcc atctcccgca agtgtc                                            26


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RT3-gadB-F forward primer for gadB

<400>  19
atgctccctt tgttgagcca                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RT3-gadB-R reverse primer for gadB

<400>  20
acgccacaaa acccaaccta                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ll16srRNA2-F forward primer for 16srRNA

<400>  21
acgagactgc cggtgataaa                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ll16srRNA2-R reverse primer for 16s rRNA

<400>  22
gagttgcagc ctacaatccg                                                   20


<210>  23
<211>  203
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized P1 promoter

<400>  23
tctttaagtt cataagtcat caaaatttgc tgacagactt tcaatagaga acttctattt       60

ctgtcagtaa ttttttccac tattttaaag aaattcactt catcaattat accataaacc      120

attttttaac agttttcttt ttacaaattg acaaaataag agtaaaatta aaatatagat      180

aaaaacttat gaaggagtcg cct                                              203


<210>  24
<211>  201
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized P3 promoter

<400>  24
gcactgattc ttttttcagt gctttttttt ataaaaattg gacaaaatag acatgttgtc       60

taaaaactat ttcagattct tgcaatcatt ttcttcttgt actatgatga aattagtaag      120

agattggagg agaattagaa gtaaagagac taaaaaattt agtcaaactg atgttgttta      180

gattagagga ggttaattat t                                                201


<210>  25
<211>  213
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized P4 promoter

<400>  25
aacttcctaa agatttcaaa atcaaagtta ctttcttgtt gttttaaatt ttacactttt       60

tgtaatgaac tgtcaaactt taaaaagtag aaaatcaatt tgcttgaatt cacaattgca      120

aaaagatata ctcaaattac aagagagaat tacaaataca acttaagtat tcaattcata      180

aagcaattgc acagttctat taaggaggaa aaa                                   213


<210>  26
<211>  203
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized P6 promoter

<400>  26
agccattttt ttgcaaaaaa agcatattag taactaacaa aaatataaac aaaagtaaaa       60

taatagaatt taatgtacga aatttattac aaatttgtat tatattatgc tataatatcc      120

tagaatcggt aatttttata agaaaatgta atcaataaaa tacatctctt gcaaaaatga      180

tttaaaatca aaaggaggac att                                              203


<210>  27
<211>  351
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized P7 promoter

<400>  27
agtgattatt ataaaagaca attctgataa agagttgttt tttttaattt cttaagcaaa       60

tcttattctt gataagcaaa ctatttttgt gctataataa aaatatcttc agggcaccgt      120

gtaattcggg accggcggta aatagggctt tgaccttatg actccgcgat tcgctacggc      180

gattgaagca gtgagaatct gctagcgaca gtaaagtctg gatggaagaa gatgaacaat      240

ttttgtagtg taatcaactt cggttggatt actgttttta gccaaaccaa aaatgtctat      300

caacttcctc gaaatttgaa gaattatttt ctcatatttg gaggtttttt t               351


