                         SEQUENCE LISTING

<110>  California Institute of Technology
 
<120>  ALLOSTERIC CONDITIONAL GUIDE RNAS FOR CELL-SELECTIVE REGULATION 
       OF CRISPR/CAS

<130>  CALTE.157WO

<150>  US 63/181808
<151>  2021-04-29

<150>  US 63/141865
<151>  2021-01-26

<160>  108   

<170>  PatentIn version 3.5

<210>  1
<211>  129
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA A

<400>  1
aactttcagt ttagcggtct gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgatcaaacg ggtaaacaaa caggataatt aaggaggcag tacccgggca ccgagtcggt      120

gcttttttt                                                              129


<210>  2
<211>  129
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA B

<400>  2
aactttcagt ttagcggtct gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgtatcatgg ggttgtgtgt tgttgtaagt gtgtgtgtgt tgccccggca ccgagtcggt      120

gcttttttt                                                              129


<210>  3
<211>  129
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA C

<400>  3
aactttcagt ttagcggtct gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgaatatagg ggaagagaaa gaagaagaga agagaaagat gtccccggca ccgagtcggt      120

gcttttttt                                                              129


<210>  4
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XA

<400>  4
tactgcctcc ttaattatcc tgtttgttta cccgtttgat                             40


<210>  5
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XB

<400>  5
caacacacac acacttacaa caacacacaa ccccatgata                             40


<210>  6
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XC

<400>  6
acatctttct cttctcttct tctttctctt cccctatatt                             40


<210>  7
<211>  165
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA A

<400>  7
catctaattc aacaagaatt gttttagagc tacaccttac gccggttcaa ttccaagtcc       60

cttccagtag caagttaaaa taaggctagt ccgttatcaa cttaacaccc tttacaaacc      120

ttcctcttcc tttaccctaa gtggcaccga gtcggtgctt ttttt                      165


<210>  8
<211>  165
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA B

<400>  8
catctaattc aacaagaatt gttttagagc tagtaatcga atcatagtaa atttcccatc       60

gtcataatag caagttaaaa taaggctagt ccgttatcaa cttcatacgg gtctgaagta      120

gttcattctt atacagtcaa gtggcaccga gtcggtgctt ttttt                      165


<210>  9
<211>  165
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA C

<400>  9
catctaattc aacaagaatt gttttagagc tagtcgttac cttatcaata tcaacctccg       60

catacactag caagttaaaa taaggctagt ccgttatcaa cttgcacata ggacccaaca      120

tgccaacaga gaagagttaa gtggcaccga gtcggtgctt ttttt                      165


<210>  10
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XA

<400>  10
agggtaaagg aagaggaagg tttgtaaagg gtgttctgga agggacttgg aattgaaccg       60

gcgtaaggtg                                                              70


<210>  11
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XB

<400>  11
gactgtataa gaatgaacta cttcagaccc gtatgttatg acgatgggaa atttactatg       60

attcgattac                                                              70


<210>  12
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XC

<400>  12
aactcttctc tgttggcatg ttgggtccta tgtgcgtgta tgcggaggtt gatattgata       60

aggtaacgac                                                              70


<210>  13
<211>  129
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA Q

<400>  13
gagtcgcgtg tagcgaagca gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgatctttgc gcgttagttt cgttcgtatt tctgtcatgt ttgcgcggca ccgagtcggt      120

gcttttttt                                                              129


<210>  14
<211>  129
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA R

<400>  14
gagtcgcgtg tagcgaagca gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgtatcgccg ggttcaagca gatgtggcat ttcagtgtag ttcccgggca ccgagtcggt      120

gcttttttt                                                              129


<210>  15
<211>  129
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA S

<400>  15
gagtcgcgtg tagcgaagca gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgtccattcg ggtttactat tacaatctta cgtgttctca ttcccgggca ccgagtcggt      120

gcttttttt                                                              129


<210>  16
<211>  129
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA T

<400>  16
gagtcgcgtg tagcgaagca gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cggataaagg gaaagatgaa gtgatgtgaa gatagagttg gatcccggca ccgagtcggt      120

gcttttttt                                                              129


<210>  17
<211>  117
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XQ

<400>  17
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgta aacatgacag aaatacgaac gaaactaacg cgcaaagatc ttttttt         117


<210>  18
<211>  117
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XR

<400>  18
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgta actacactga aatgccacat ctgcttgaac ccggcgatac ttttttt         117


<210>  19
<211>  117
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XS

<400>  19
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgta atgagaacac gtaagattgt aatagtaaac ccgaatggac ttttttt         117


<210>  20
<211>  117
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XT

<400>  20
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgtt ccaactctat cttcacatca cttcatcttt ccctttatcc ttttttt         117


<210>  21
<211>  117
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XQ - 40 nt

<400>  21
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgta aacatgacag aaatacgaac gaaactaacg cgcaaagatc ttttttt         117


<210>  22
<211>  127
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  50 nt Trigger - Center

<400>  22
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgtg ccgaaaacat gacagaaata cgaacgaaac taacgcgcaa agattccagc      120

ttttttt                                                                127


<210>  23
<211>  127
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  50 nt Trigger - 40 nt XQ at 3'

<400>  23
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgtg ccgatccaga aacatgacag aaatacgaac gaaactaacg cgcaaagatc      120

ttttttt                                                                127


<210>  24
<211>  127
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  50 nt Trigger - 40 nt XQ at 5'

<400>  24
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgta aacatgacag aaatacgaac gaaactaacg cgcaaagatg ccgatccagc      120

ttttttt                                                                127


<210>  25
<211>  147
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  70 nt Trigger - Center

<400>  25
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgtg ccgagccgag ccgaaaacat gacagaaata cgaacgaaac taacgcgcaa      120

agatgccgag ccgatccagc ttttttt                                          147


<210>  26
<211>  147
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  70 nt Trigger - 40 nt XQ at 3'

<400>  26
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgtg ccgatccagg ccgatccagg ccgatccaga aacatgacag aaatacgaac      120

gaaactaacg cgcaaagatc ttttttt                                          147


<210>  27
<211>  147
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  70 nt Trigger - 40 nt XQ at 5'

<400>  27
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgta aacatgacag aaatacgaac gaaactaacg cgcaaagatg ccgatccagg      120

ccgatccagg ccgatccagc ttttttt                                          147


<210>  28
<211>  177
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  100 nt Trigger - Center

<400>  28
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgtg ccgatccagg ccgatccagg ccgatccaga aacatgacag aaatacgaac      120

gaaactaacg cgcaaagatg ccgatccagg ccgatccagg ccgatccagc ttttttt         177


<210>  29
<211>  177
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  100 nt Trigger - 40 nt XQ at 3'

<400>  29
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgtg ccgatccagg ccgatccagg ccgatccagg ccgatccagg ccgatccagg      120

ccgatccaga aacatgacag aaatacgaac gaaactaacg cgcaaagatc ttttttt         177


<210>  30
<211>  177
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  100 nt Trigger - 40 nt XQ at 5'

<400>  30
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgta aacatgacag aaatacgaac gaaactaacg cgcaaagatg ccgatccagg      120

ccgatccagg ccgatccagg ccgatccagg ccgatccagg ccgatccagc ttttttt         177


<210>  31
<211>  227
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  150 nt Trigger - Center

<400>  31
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgtg ccgatccagg ccgatccagg ccgagccgat ccaggccgat ccaggccgat      120

ccagaaacat gacagaaata cgaacgaaac taacgcgcaa agatgccgat ccaggccgat      180

ccaggccgag ccgatccagg ccgatccagg ccgatccagc ttttttt                    227


<210>  32
<211>  227
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  150 nt Trigger - 40 nt XQ at 3'

<400>  32
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgtg ccgatccagg ccgatccagg ccgatccagg ccgatccagg ccgatccagg      120

ccgatccagg ccgatccagg ccgatccagg ccgatccagg ccgatccagg ccgatccaga      180

aacatgacag aaatacgaac gaaactaacg cgcaaagatc ttttttt                    227


<210>  33
<211>  227
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  150 nt Trigger - 40 nt XQ at 5'

<400>  33
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgta aacatgacag aaatacgaac gaaactaacg cgcaaagatg ccgatccagg      120

ccgatccagg ccgatccagg ccgatccagg ccgatccagg ccgatccagg ccgatccagg      180

ccgatccagg ccgatccagg ccgatccagg ccgatccagc ttttttt                    227


<210>  34
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA M

<400>  34
gagtcgcgtg tagcgaagca gtttaagagc tatgctggaa acagcatagc aagtttaaat       60

aaggctagtc cgttatcagc accttttttt                                        90


<210>  35
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA N

<400>  35
gagtcgcgtg tagcgaagca gtttaagagc tatgctggaa acagcatagc aagtttaaat       60

aaggctagtc cgttatcagg cccttttttt                                        90


<210>  36
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA O

<400>  36
gagtcgcgtg tagcgaagca gtttaagagc tatgctggaa acagcatagc aagtttaaat       60

aaggctagtc cgttatcacc cacttttttt                                        90


<210>  37
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XM

<400>  37
gtgcggcacc gagtcggtgc ttttttt                                           27


<210>  38
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XN

<400>  38
ggccggcacc gagtcggtgc ttttttt                                           27


<210>  39
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XO

<400>  39
tgggggcacc gagtcggtgc ttttttt                                           27


<210>  40
<211>  96
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA M

<400>  40
gagtcgcgtg tagcgaagca gtttaagagc tatgctggaa acagcatagc aagtttaaat       60

aaggctagtc cgttatcagc acatcccact tttttt                                 96


<210>  41
<211>  96
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA N

<400>  41
gagtcgcgtg tagcgaagca gtttaagagc tatgctggaa acagcatagc aagtttaaat       60

aaggctagtc cgttatcagg ccaggttcct tttttt                                 96


<210>  42
<211>  96
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA O

<400>  42
gagtcgcgtg tagcgaagca gtttaagagc tatgctggaa acagcatagc aagtttaaat       60

aaggctagtc cgttatcacc cagaacacct tttttt                                 96


<210>  43
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XM

<400>  43
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgtt gggatgtgcg gcaccgagtc ggtgcttttt tt                         102


<210>  44
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XN

<400>  44
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgtg aacctggccg gcaccgagtc ggtgcttttt tt                         102


<210>  45
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XO

<400>  45
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgtg tgttctgggg gcaccgagtc ggtgcttttt tt                         102


<210>  46
<211>  96
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA M

<400>  46
gagtcgcgtg tagcgaagca gtttaagagc tatgctggaa acagcatagc aagtttaaat       60

aaggctagtc cgttatcagc acatcccact tttttt                                 96


<210>  47
<211>  96
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA N

<400>  47
gagtcgcgtg tagcgaagca gtttaagagc tatgctggaa acagcatagc aagtttaaat       60

aaggctagtc cgttatcagg ccaggttcct tttttt                                 96


<210>  48
<211>  96
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA O

<400>  48
gagtcgcgtg tagcgaagca gtttaagagc tatgctggaa acagcatagc aagtttaaat       60

aaggctagtc cgttatcacc cagaacacct tttttt                                 96


<210>  49
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XM

<400>  49
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgtt gggatgtgcg gcaccgagtc ggtgcttttt tt                         102


<210>  50
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XN

<400>  50
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgtg aacctggccg gcaccgagtc ggtgcttttt tt                         102


<210>  51
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XO

<400>  51
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgtg tgttctgggg gcaccgagtc ggtgcttttt tt                         102


<210>  52
<211>  94
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA M

<400>  52
gagtcgcgtg tagcgaagca gtttaagagc tatgctggaa acagcatagc aagtttaaat       60

aaggctagtc cgttatcagc acatcccttt tttt                                   94


<210>  53
<211>  94
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA N

<400>  53
gagtcgcgtg tagcgaagca gtttaagagc tatgctggaa acagcatagc aagtttaaat       60

aaggctagtc cgttatcagg ccaggtcttt tttt                                   94


<210>  54
<211>  94
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA O

<400>  54
gagtcgcgtg tagcgaagca gtttaagagc tatgctggaa acagcatagc aagtttaaat       60

aaggctagtc cgttatcacc cagaaccttt tttt                                   94


<210>  55
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XM

<400>  55
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgtg gatgtgcggc accgagtcgg tgcttttttt                            100


<210>  56
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XN

<400>  56
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgta cctggccggc accgagtcgg tgcttttttt                            100


<210>  57
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XO

<400>  57
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgtg ttctgggggc accgagtcgg tgcttttttt                            100


<210>  58
<211>  92
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA M

<400>  58
gagtcgcgtg tagcgaagca gtttaagagc tatgctggaa acagcatagc aagtttaaat       60

aaggctagtc cgttatcagc acatcttttt tt                                     92


<210>  59
<211>  92
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA N

<400>  59
gagtcgcgtg tagcgaagca gtttaagagc tatgctggaa acagcatagc aagtttaaat       60

aaggctagtc cgttatcagg ccagcttttt tt                                     92


<210>  60
<211>  92
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA O

<400>  60
gagtcgcgtg tagcgaagca gtttaagagc tatgctggaa acagcatagc aagtttaaat       60

aaggctagtc cgttatcacc cagacttttt tt                                     92


<210>  61
<211>  98
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XM

<400>  61
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgta tgtgcggcac cgagtcggtg cttttttt                               98


<210>  62
<211>  98
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XN

<400>  62
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgtc tggccggcac cgagtcggtg cttttttt                               98


<210>  63
<211>  98
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XO

<400>  63
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgtt ctgggggcac cgagtcggtg cttttttt                               98


<210>  64
<211>  125
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA 40

<400>  64
agtcgcgtgt agcgaagcag tttaagagct atgctggaaa cagcatagca agtttaaata       60

aggctagtcc gttatcacat atcccatcca cctccacctc cacctccaca ttcccacctt      120

ttttt                                                                  125


<210>  65
<211>  115
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA 30

<400>  65
agtcgcgtgt agcgaagcag tttaagagct atgctggaaa cagcatagca agtttaaata       60

aggctagtcc gttatcacat atcccatcca cctccacctc cacctccctt ttttt           115


<210>  66
<211>  105
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA 20

<400>  66
agtcgcgtgt agcgaagcag tttaagagct atgctggaaa cagcatagca agtttaaata       60

aggctagtcc gttatcacat atcccatcca cctccacctt ttttt                      105


<210>  67
<211>  95
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA 10

<400>  67
agtcgcgtgt agcgaagcag tttaagagct atgctggaaa cagcatagca agtttaaata       60

aggctagtcc gttatcacat atcccatctt ttttt                                  95


<210>  68
<211>  132
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger X40

<400>  68
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgtg tgggaatgtg gaggtggagg tggaggtgga tgggatatgg gcaccgagtc      120

ggtgcttttt tt                                                          132


<210>  69
<211>  122
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger X30

<400>  69
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgtg gaggtggagg tggaggtgga tgggatatgg gcaccgagtc ggtgcttttt      120

tt                                                                     122


<210>  70
<211>  113
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger X20

<400>  70
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgtg tggaggtgga tgggatatgg gcaccgagtc ggtgcttttt ttt             113


<210>  71
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger X10

<400>  71
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgta tgggatatgg gcaccgagtc ggtgcttttt tt                         102


<210>  72
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Standard gRNA

<400>  72
gagtcgcgtg tagcgaagca gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  73
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  No-target gRNA

<400>  73
gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt ttt                                               83


<210>  74
<211>  95
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  8 nt deletion

<400>  74
gagtcgcgtg tagcgaagca gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgctt ttttt                                  95


<210>  75
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  23 nt deletion

<400>  75
gagtcgcgtg tagcgaagca gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttcttttttt                                                   80


<210>  76
<211>  71
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  32 nt deletion

<400>  76
gagtcgcgtg tagcgaagca gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgtctttttt t                                                            71


<210>  77
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  45 nt deletion

<400>  77
gagtcgcgtg tagcgaagca gttttagagc tagaaatagc aagttaaaat cttttttt         58


<210>  78
<211>  129
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA  A

<400>  78
aactttcagt ttagcggtct gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgatcaaacg ggtaaacaaa caggataatt aaggaggcag tacccgggca ccgagtcggt      120

gcttttttt                                                              129


<210>  79
<211>  129
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA E

<400>  79
aactttcagt ttagcggtct gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgacacaagg ggaaattaac aacacaacac acacaacaca ggccccggca ccgagtcggt      120

gcttttttt                                                              129


<210>  80
<211>  1849
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XA

<400>  80
tggctaaaga aagaggagaa aaggtttatg gtagcaggtc atgcctctgg cagccccgca       60

ttcgggaccg cctctcattc gaattgcgaa catgaagaga tccacctcgc cggctcgatc      120

cagccgcatg gcgcgcttct ggtcgtcagc gaacatgatc atcgcgtcat ccaggccagc      180

gccaacgccg cggaatttct gaatctcgga agcgtactcg gcgttccgct cgccgagatc      240

gacggcgatc tgttgatcaa gatcctgccg catctcgatc ccaccgccga aggcatgccg      300

gtcgcggtgc gctgccggat cggcaatccc tctacggagt actgcggtct gatgcatcgg      360

cctccggaag gcgggctgat catcgaactc gaacgtgccg gcccgtcgat cgatctgtca      420

ggcacgctgg cgccggcgct ggagcggatc cgcacggcgg gttcactgcg cgcgctgtgc      480

gatgacaccg tgctgctgtt tcagcagtgc accggctacg accgggtgat ggtgtatcgt      540

ttcgatgagc aaggccacgg cctggtattc tccgagtgcc atgtgcctgg gctcgaatcc      600

tatttcggca accgctatcc gtcgtcgact gtcccgcaga tggcgcggca gctgtacgtg      660

cggcagcgcg tccgcgtgct ggtcgacgtc acctatcagc cggtgccgct ggagccgcgg      720

ctgtcgccgc tgaccgggcg cgatctcgac atgtcgggct gcttcctgcg ctcgatgtcg      780

ccgtgccatc tgcagttcct gaaggacatg ggcgtgcgcg ccaccctggc ggtgtcgctg      840

gtggtcggcg gcaagctgtg gggcctggtt gtctgtcacc attatctgcc gcgcttcatc      900

cgtttcgagc tgcgggcgat ctgcaaacgg ctcgccgaaa ggatcgcgac gcggatcacc      960

gcgcttgaga gcgaattcgg tggtggtggt tctggtggtg gtggttctat gagtgtcaac     1020

ttagcttccc agttgcggga agggacgaaa aaatcccact ccatggcgga gaacgtcggc     1080

tttgtcaaat gcttcctcaa gggcgttgtc gagaaaaatt cctaccgtaa gctggttggc     1140

aatctctact ttgtctacag tgccatggaa gaggaaatgg caaaatttaa ggaccatccc     1200

atcctcagcc acatttactt ccccgaactc aaccgcaaac aaagcctaga gcaagacctg     1260

caattctatt acggctccaa ctggcggcaa gaagtgaaaa tttctgccgc tggccaagcc     1320

tatgtggacc gagtccggca agtggccgct acggcccctg aattgttggt ggcccattcc     1380

tacacccgtt acctggggga tctttccggc ggtcaaattc tcaagaaaat tgcccaaaat     1440

gccatgaatc tccacgatgg tggcacagct ttctatgaat ttgccgacat tgatgacgaa     1500

aaggctttta aaaataccta ccgtcaagct atgaatgatc tgcccattga ccaagccacc     1560

gccgaacgga ttgtggatga agccaatgac gcctttgcca tgaacatgaa aatgttcaac     1620

gaacttgaag gcaacctgat caaggcgatc ggcattatgg tgttcaacag cctcacccgt     1680

cgccgcagtc aaggcagcac cgaagttggc ctcgccacct ccgaaggcta gtaaacgtcg     1740

actctcgagt gagattgttg acggtaccgt atttttactg cctccttaat tatcctgttt     1800

gtttacccgt ttgatcgcaa aaaaccccgc ttcggcgggg ttttttcgc                 1849


<210>  81
<211>  1849
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XE

<400>  81
tggctaaaga aagaggagaa aaggtttatg gtagcaggtc atgcctctgg cagccccgca       60

ttcgggaccg cctctcattc gaattgcgaa catgaagaga tccacctcgc cggctcgatc      120

cagccgcatg gcgcgcttct ggtcgtcagc gaacatgatc atcgcgtcat ccaggccagc      180

gccaacgccg cggaatttct gaatctcgga agcgtactcg gcgttccgct cgccgagatc      240

gacggcgatc tgttgatcaa gatcctgccg catctcgatc ccaccgccga aggcatgccg      300

gtcgcggtgc gctgccggat cggcaatccc tctacggagt actgcggtct gatgcatcgg      360

cctccggaag gcgggctgat catcgaactc gaacgtgccg gcccgtcgat cgatctgtca      420

ggcacgctgg cgccggcgct ggagcggatc cgcacggcgg gttcactgcg cgcgctgtgc      480

gatgacaccg tgctgctgtt tcagcagtgc accggctacg accgggtgat ggtgtatcgt      540

ttcgatgagc aaggccacgg cctggtattc tccgagtgcc atgtgcctgg gctcgaatcc      600

tatttcggca accgctatcc gtcgtcgact gtcccgcaga tggcgcggca gctgtacgtg      660

cggcagcgcg tccgcgtgct ggtcgacgtc acctatcagc cggtgccgct ggagccgcgg      720

ctgtcgccgc tgaccgggcg cgatctcgac atgtcgggct gcttcctgcg ctcgatgtcg      780

ccgtgccatc tgcagttcct gaaggacatg ggcgtgcgcg ccaccctggc ggtgtcgctg      840

gtggtcggcg gcaagctgtg gggcctggtt gtctgtcacc attatctgcc gcgcttcatc      900

cgtttcgagc tgcgggcgat ctgcaaacgg ctcgccgaaa ggatcgcgac gcggatcacc      960

gcgcttgaga gcgaattcgg tggtggtggt tctggtggtg gtggttctat gagtgtcaac     1020

ttagcttccc agttgcggga agggacgaaa aaatcccact ccatggcgga gaacgtcggc     1080

tttgtcaaat gcttcctcaa gggcgttgtc gagaaaaatt cctaccgtaa gctggttggc     1140

aatctctact ttgtctacag tgccatggaa gaggaaatgg caaaatttaa ggaccatccc     1200

atcctcagcc acatttactt ccccgaactc aaccgcaaac aaagcctaga gcaagacctg     1260

caattctatt acggctccaa ctggcggcaa gaagtgaaaa tttctgccgc tggccaagcc     1320

tatgtggacc gagtccggca agtggccgct acggcccctg aattgttggt ggcccattcc     1380

tacacccgtt acctggggga tctttccggc ggtcaaattc tcaagaaaat tgcccaaaat     1440

gccatgaatc tccacgatgg tggcacagct ttctatgaat ttgccgacat tgatgacgaa     1500

aaggctttta aaaataccta ccgtcaagct atgaatgatc tgcccattga ccaagccacc     1560

gccgaacgga ttgtggatga agccaatgac gcctttgcca tgaacatgaa aatgttcaac     1620

gaacttgaag gcaacctgat caaggcgatc ggcattatgg tgttcaacag cctcacccgt     1680

cgccgcagtc aaggcagcac cgaagttggc ctcgccacct ccgaaggcta gtaaacgtcg     1740

actctcgagt gagattgttg acggtaccgt attttcctgt gttgtgtgtg ttgtgttgtt     1800

aatttcccct tgtgtcgcaa aaaaccccgc ttcggcgggg ttttttcgc                 1849


<210>  82
<211>  129
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA

<400>  82
gagtcgcgtg tagcgaagca gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgtccattcg ggtttactat tacaatctta cgtgttctca ttcccgggca ccgagtcggt      120

gcttttttt                                                              129


<210>  83
<211>  117
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Non-cognate trigger (X')

<400>  83
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgta aacatgacag aaatacgaac gaaactaacg cgcaaagatc ttttttt         117


<210>  84
<211>  117
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cognate-trigger (X)

<400>  84
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgta atgagaacac gtaagattgt aatagtaaac ccgaatggac ttttttt         117


<210>  85
<211>  96
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA

<400>  85
gagtcgcgtg tagcgaagca gtttaagagc tatgctggaa acagcatagc aagtttaaat       60

aaggctagtc cgttatcagg ccaggttcct tttttt                                 96


<210>  86
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Non-cognate trigger (X')

<400>  86
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgtt gggatgtgcg gcaccgagtc ggtgcttttt tt                         102


<210>  87
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cognate trigger (X)

<400>  87
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgtg aacctggccg gcaccgagtc ggtgcttttt tt                         102


<210>  88
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA M

<400>  88
catctaattc aacaagaatt gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcagc ac                                                           72


<210>  89
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA N

<400>  89
catctaattc aacaagaatt gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcagg cc                                                           72


<210>  90
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cgRNA O

<400>  90
catctaattc aacaagaatt gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcacc ca                                                           72


<210>  91
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XM

<400>  91
gtgcggcacc gagtcggtg                                                    19


<210>  92
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XN

<400>  92
ggccggcacc gagtcggtg                                                    19


<210>  93
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger XO

<400>  93
tgggggcacc gagtcggtg                                                    19


<210>  94
<211>  96
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cg5

<400>  94
gagtcgcgtg tagcgaagca gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgtcccatcg tccgtcccat caatttccct tttttt                                 96


<210>  95
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cg3

<400>  95
aatataatac gggacggacg ggcaccgagt cggtgctttt ttt                         43


<210>  96
<211>  97
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger

<400>  96
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgtc gtccgtcccg tattatattc ttttttt                                97


<210>  97
<211>  106
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cg5

<400>  97
gagtcgcgtg tagcgaagca gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgatatatac aaacacaaca cacacacaac aacaaacact tttttt                     106


<210>  98
<211>  132
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cg3

<400>  98
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgtt gtctagaatt gtttgttgtt gtgtgtgtgt tgtgtttgtg gcaccgagtc      120

ggtgcttttt tt                                                          132


<210>  99
<211>  117
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger

<400>  99
agtcaggcca cttgtgccac ggtttgagca aaccgtgctg cctgtagctc cgccaataat       60

gggaggcgta caaacacaac acacacacaa caacaaacaa ttctagacac ttttttt         117


<210>  100
<211>  146
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cg5

<400>  100
aactttcagt ttagcggtct gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgatatatac aaacacaaca cacacacaac aacaaacaca acccaaccag agcgcaaaaa      120

accccgcttc ggcggggttt tttcgc                                           146


<210>  101
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cg3

<400>  101
tgtctagaat tgtttgttgt tgtgtgtgtg ttgtgtttgt ggcaccgagt cggtgctttt       60

tttcgcc                                                                 67


<210>  102
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  No-trigger control

<400>  102
cgcaaaaaac cccgcttcgg cggggttttt tcgc                                   34


<210>  103
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger X (Mechanism 4B)

<400>  103
ggttgggttg tgtttgttgt tgtgtgtgtg ttgtgtttgt cgcaaaaaac cccgcttcgg       60

cggggttttt tcgc                                                         74


<210>  104
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger X (Mechanism 4C)

<400>  104
acaaacacaa cacacacaca acaacaaaca attctagaca cgcaaaaaac cccgcttcgg       60

cggggttttt tcgc                                                         74


<210>  105
<211>  146
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cg5

<400>  105
ttgtttaagg ctatggtgag aactttcagt ttagcggtct gttttagagc tagaaatagc       60

aagttaaaat aaggctagtc cgtaaataaa agcccaccct caccatagag agcgcaaaaa      120

accccgcttc ggcggggttt tttcgc                                           146


<210>  106
<211>  95
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cg3

<400>  106
ccaccctcac cataggtgct atggtgaggg tgggctttgg caccgagtcg gtgctttttt       60

tcgcaaaaaa ccccgcttcg gcggggtttt ttcgc                                  95


<210>  107
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  No-trigger control

<400>  107
cgcaaaaaac cccgcttcgg cggggttttt tcgc                                   34


<210>  108
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Trigger X

<400>  108
ctcaccatag ccttgaacaa cgcaaaaaac cccgcttcgg cggggttttt tcgc             54


