                         SEQUENCE LISTING

<110>  10x Genomics, Inc.
 
<120>  METHOD FOR TRANSPOSASE MEDIATED SPATIAL TAGGING AND ANALYZING 
       GENOMIC DNA IN A BIOLOGICAL SAMPLE

<130>  47706-0292WO1

<150>  63/143,438
<151>  2020-01-29

<150>  63/166,708
<151>  2021-03-26

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  476
<212>  PRT
<213>  Escherichia coli

<400>  1

Met Ile Thr Ser Ala Leu His Arg Ala Ala Asp Trp Ala Lys Ser Val 
1               5                   10                  15      


Phe Ser Ser Ala Ala Leu Gly Asp Pro Arg Arg Thr Ala Arg Leu Val 
            20                  25                  30          


Asn Val Ala Ala Gln Leu Ala Lys Tyr Ser Gly Lys Ser Ile Thr Ile 
        35                  40                  45              


Ser Ser Glu Gly Ser Glu Ala Met Gln Glu Gly Ala Tyr Arg Phe Ile 
    50                  55                  60                  


Arg Asn Pro Asn Val Ser Ala Glu Ala Ile Arg Lys Ala Gly Ala Met 
65                  70                  75                  80  


Gln Thr Val Lys Leu Ala Gln Glu Phe Pro Glu Leu Leu Ala Ile Glu 
                85                  90                  95      


Asp Thr Thr Ser Leu Ser Tyr Arg His Gln Val Ala Glu Glu Leu Gly 
            100                 105                 110         


Lys Leu Gly Ser Ile Gln Asp Lys Ser Arg Gly Trp Trp Val His Ser 
        115                 120                 125             


Val Leu Leu Leu Glu Ala Thr Thr Phe Arg Thr Val Gly Leu Leu His 
    130                 135                 140                 


Gln Glu Trp Trp Met Arg Pro Asp Asp Pro Ala Asp Ala Asp Glu Lys 
145                 150                 155                 160 


Glu Ser Gly Lys Trp Leu Ala Ala Ala Ala Thr Ser Arg Leu Arg Met 
                165                 170                 175     


Gly Ser Met Met Ser Asn Val Ile Ala Val Cys Asp Arg Glu Ala Asp 
            180                 185                 190         


Ile His Ala Tyr Leu Gln Asp Lys Leu Ala His Asn Glu Arg Phe Val 
        195                 200                 205             


Val Arg Ser Lys His Pro Arg Lys Asp Val Glu Ser Gly Leu Tyr Leu 
    210                 215                 220                 


Tyr Asp His Leu Lys Asn Gln Pro Glu Leu Gly Gly Tyr Gln Ile Ser 
225                 230                 235                 240 


Ile Pro Gln Lys Gly Val Val Asp Lys Arg Gly Lys Arg Lys Asn Arg 
                245                 250                 255     


Pro Ala Arg Lys Ala Ser Leu Ser Leu Arg Ser Gly Arg Ile Thr Leu 
            260                 265                 270         


Lys Gln Gly Asn Ile Thr Leu Asn Ala Val Leu Ala Glu Glu Ile Asn 
        275                 280                 285             


Pro Pro Lys Gly Glu Thr Pro Leu Lys Trp Leu Leu Leu Thr Ser Glu 
    290                 295                 300                 


Pro Val Glu Ser Leu Ala Gln Ala Leu Arg Val Ile Asp Ile Tyr Thr 
305                 310                 315                 320 


His Arg Trp Arg Ile Glu Glu Phe His Lys Ala Trp Lys Thr Gly Ala 
                325                 330                 335     


Gly Ala Glu Arg Gln Arg Met Glu Glu Pro Asp Asn Leu Glu Arg Met 
            340                 345                 350         


Val Ser Ile Leu Ser Phe Val Ala Val Arg Leu Leu Gln Leu Arg Glu 
        355                 360                 365             


Ser Phe Thr Leu Pro Gln Ala Leu Arg Ala Gln Gly Leu Leu Lys Glu 
    370                 375                 380                 


Ala Glu His Val Glu Ser Gln Ser Ala Glu Thr Val Leu Thr Pro Asp 
385                 390                 395                 400 


Glu Cys Gln Leu Leu Gly Tyr Leu Asp Lys Gly Lys Arg Lys Arg Lys 
                405                 410                 415     


Glu Lys Ala Gly Ser Leu Gln Trp Ala Tyr Met Ala Ile Ala Arg Leu 
            420                 425                 430         


Gly Gly Phe Met Asp Ser Lys Arg Thr Gly Ile Ala Ser Trp Gly Ala 
        435                 440                 445             


Leu Trp Glu Gly Trp Glu Ala Leu Gln Ser Lys Leu Asp Gly Phe Leu 
    450                 455                 460                 


Ala Ala Lys Asp Leu Met Ala Gln Gly Ile Lys Ile 
465                 470                 475     


<210>  2
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Tn5 Mosaic End Sequence

<400>  2
ctgtctctta tacacatct                                                    19


<210>  3
<211>  662
<212>  PRT
<213>  Bacteriophage Mu

<400>  3

Met Lys Glu Trp Tyr Thr Ala Lys Glu Leu Leu Gly Leu Ala Gly Leu 
1               5                   10                  15      


Pro Lys Gln Ala Thr Asn Ile Thr Arg Lys Ala Gln Arg Glu Gly Trp 
            20                  25                  30          


Glu Phe Arg Gln Val Ala Gly Thr Lys Gly Val Ser Phe Glu Phe Asn 
        35                  40                  45              


Ile Lys Ser Phe Pro Val Ala Leu Arg Ala Glu Ile Leu Leu Gln Gln 
    50                  55                  60                  


Gly Arg Ile Glu Thr Ser Gln Gly Tyr Phe Glu Ile Ala Arg Pro Thr 
65                  70                  75                  80  


Leu Glu Ala His Asp Tyr Asp Arg Glu Ala Leu Trp Ser Lys Trp Asp 
                85                  90                  95      


Asn Ala Ser Asp Ser Gln Arg Arg Leu Ala Glu Lys Trp Leu Pro Ala 
            100                 105                 110         


Val Gln Ala Ala Asp Glu Met Leu Asn Gln Gly Ile Ser Thr Lys Thr 
        115                 120                 125             


Ala Phe Ala Thr Val Ala Gly His Tyr Gln Val Ser Ala Ser Thr Leu 
    130                 135                 140                 


Arg Asp Lys Tyr Tyr Gln Val Gln Lys Phe Ala Lys Pro Asp Trp Ala 
145                 150                 155                 160 


Ala Ala Leu Val Asp Gly Arg Gly Ala Ser Arg Arg Asn Val His Lys 
                165                 170                 175     


Ser Glu Phe Asp Glu Asp Ala Trp Gln Phe Leu Ile Ala Asp Tyr Leu 
            180                 185                 190         


Arg Pro Glu Lys Pro Ala Phe Arg Lys Cys Tyr Glu Arg Leu Glu Leu 
        195                 200                 205             


Ala Ala Arg Glu His Gly Trp Ser Ile Pro Ser Arg Ala Thr Ala Phe 
    210                 215                 220                 


Arg Arg Ile Gln Gln Leu Asp Glu Ala Met Val Val Ala Cys Arg Glu 
225                 230                 235                 240 


Gly Glu His Ala Leu Met His Leu Ile Pro Ala Gln Gln Arg Thr Val 
                245                 250                 255     


Glu His Leu Asp Ala Met Gln Trp Ile Asn Gly Asp Gly Tyr Leu His 
            260                 265                 270         


Asn Val Phe Val Arg Trp Phe Asn Gly Asp Val Ile Arg Pro Lys Thr 
        275                 280                 285             


Trp Phe Trp Gln Asp Val Lys Thr Arg Lys Ile Leu Gly Trp Arg Cys 
    290                 295                 300                 


Asp Val Ser Glu Asn Ile Asp Ser Ile Arg Leu Ser Phe Met Asp Val 
305                 310                 315                 320 


Val Thr Arg Tyr Gly Ile Pro Glu Asp Phe His Ile Thr Ile Asp Asn 
                325                 330                 335     


Thr Arg Gly Ala Ala Asn Lys Trp Leu Thr Gly Gly Ala Pro Asn Arg 
            340                 345                 350         


Tyr Arg Phe Lys Val Lys Glu Asp Asp Pro Lys Gly Leu Phe Leu Leu 
        355                 360                 365             


Met Gly Ala Lys Met His Trp Thr Ser Val Val Ala Gly Lys Gly Trp 
    370                 375                 380                 


Gly Gln Ala Lys Pro Val Glu Arg Ala Phe Gly Val Gly Gly Leu Glu 
385                 390                 395                 400 


Glu Tyr Val Asp Lys His Pro Ala Leu Ala Gly Ala Tyr Thr Gly Pro 
                405                 410                 415     


Asn Pro Gln Ala Lys Pro Asp Asn Tyr Gly Asp Arg Ala Val Asp Ala 
            420                 425                 430         


Glu Leu Phe Leu Lys Thr Leu Ala Glu Gly Val Ala Met Phe Asn Ala 
        435                 440                 445             


Arg Thr Gly Arg Glu Thr Glu Met Cys Gly Gly Lys Leu Ser Phe Asp 
    450                 455                 460                 


Asp Val Phe Glu Arg Glu Tyr Ala Arg Thr Ile Val Arg Lys Pro Thr 
465                 470                 475                 480 


Glu Glu Gln Lys Arg Met Leu Leu Leu Pro Ala Glu Ala Val Asn Val 
                485                 490                 495     


Ser Arg Lys Gly Glu Phe Ala Leu Lys Val Gly Gly Ser Leu Lys Gly 
            500                 505                 510         


Ala Lys Asn Val Tyr Tyr Asn Met Ala Leu Met Asn Ala Gly Val Lys 
        515                 520                 525             


Lys Val Val Val Arg Phe Asp Pro Gln Gln Leu His Ser Thr Val Tyr 
    530                 535                 540                 


Cys Tyr Thr Leu Asp Gly Arg Phe Ile Cys Glu Ala Glu Cys Leu Ala 
545                 550                 555                 560 


Pro Val Ala Phe Asn Asp Ala Ala Ala Gly Arg Glu Tyr Arg Arg Arg 
                565                 570                 575     


Gln Lys Gln Leu Lys Ser Ala Thr Lys Ala Ala Ile Lys Ala Gln Lys 
            580                 585                 590         


Gln Met Asp Ala Leu Glu Val Ala Glu Leu Leu Pro Gln Ile Ala Glu 
        595                 600                 605             


Pro Glu Ala Pro Glu Ser Arg Ile Val Gly Ile Phe Arg Pro Ser Gly 
    610                 615                 620                 


Asn Thr Glu Arg Val Lys Asn Gln Glu Arg Asp Asp Glu Tyr Glu Thr 
625                 630                 635                 640 


Glu Arg Asp Glu Tyr Leu Asn His Ser Leu Asp Ile Leu Glu Gln Asn 
                645                 650                 655     


Arg Arg Lys Lys Ala Ile 
            660         


<210>  4
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  Mu Transposase Recognition Sequence

<400>  4
tgaagcggcg cacgaaaaac gcgaaag                                           27


<210>  5
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  Mu Transposase Recognition Sequence

<400>  5
gcgtttcacg ataaatgcga aaa                                               23


<210>  6
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  Mu Transposase Recognition Sequence

<400>  6
ctgtttcatt tgaagcgcga aag                                               23


<210>  7
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  Mu Transposase Recognition Sequence

<400>  7
tgtattgatt cacttgaagt acgaaaa                                           27


<210>  8
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  Mu Transposase Recognition Sequence

<400>  8
ccttaatcaa tgaaacgcga aag                                               23


<210>  9
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  Mu Transposase Recognition Sequence

<400>  9
ttgtttcatt gaaaatacga aaa                                               23


<210>  10
<211>  458
<212>  PRT
<213>  Vibrio harveyi

<400>  10

Met Thr His Ser Asp Ala Lys Leu Trp Ala Gln Glu Gln Phe Gly Gln 
1               5                   10                  15      


Ala Gln Leu Lys Asp Pro Arg Arg Thr Gln Arg Leu Ile Ser Leu Ala 
            20                  25                  30          


Thr Ser Ile Ala Asn Gln Pro Gly Val Ser Val Ala Lys Leu Pro Phe 
        35                  40                  45              


Ser Pro Ala Asp Met Glu Gly Ala Tyr Arg Phe Ile Arg Asn Glu Asn 
    50                  55                  60                  


Ile Asn Ala Glu Asp Ile Ala Glu Ala Gly Phe Gln Ser Thr Val Ser 
65                  70                  75                  80  


Arg Ala Asn Glu His Lys Glu Leu Leu Ala Leu Glu Asp Thr Thr Thr 
                85                  90                  95      


Leu Ser Phe Pro His Arg Ser Ile Lys Glu Glu Leu Gly His Thr Asn 
            100                 105                 110         


Gln Gly Asp Arg Thr Arg Ala Leu His Val His Ser Thr Leu Leu Phe 
        115                 120                 125             


Ala Pro Gln Ser Gln Thr Ile Val Gly Leu Ile Glu Gln Gln Arg Trp 
    130                 135                 140                 


Ser Glu Asp Ile Thr Lys Arg Gly Gln Lys His Gln His Ala Thr Arg 
145                 150                 155                 160 


Pro Tyr Lys Glu Lys Glu Ser Tyr Lys Trp Glu Gln Ala Ser Arg Arg 
                165                 170                 175     


Val Val Glu Arg Leu Gly Asp Lys Met Leu Asp Val Ile Ser Val Cys 
            180                 185                 190         


Asp Arg Glu Ala Asp Leu Phe Glu Tyr Leu Thr Tyr Lys Arg Gln His 
        195                 200                 205             


Gln Gln Arg Phe Val Val Arg Ser Met Gln Ser Arg Cys Leu Glu Glu 
    210                 215                 220                 


His Ala Gln Lys Leu Tyr Asp Tyr Ala Gln Ala Leu Pro Ser Val Glu 
225                 230                 235                 240 


Thr Lys Ala Leu Thr Ile Pro Gln Lys Gly Gly Arg Lys Ala Arg Asn 
                245                 250                 255     


Val Lys Leu Asp Val Lys Tyr Gly Gln Val Thr Leu Lys Ala Pro Ala 
            260                 265                 270         


Asn Lys Lys Glu His Ala Gly Ile Pro Val Tyr Tyr Val Gly Cys Leu 
        275                 280                 285             


Glu Gln Gly Thr Ser Lys Asp Leu Ala Trp His Leu Leu Thr Ser Glu 
    290                 295                 300                 


Pro Ile Asn Asn Val Asp Asp Ala Met Arg Ile Ile Gly Tyr Tyr Glu 
305                 310                 315                 320 


Arg Arg Trp Leu Ile Glu Asp Phe His Lys Val Trp Lys Ser Glu Gly 
                325                 330                 335     


Thr Asp Val Glu Ser Leu Arg Leu Gln Ser Lys Asp Asn Leu Glu Arg 
            340                 345                 350         


Leu Ser Val Ile Tyr Ala Phe Val Ala Thr Arg Leu Leu Ala Leu Arg 
        355                 360                 365             


Phe Met Lys Glu Val Asp Glu Leu Thr Lys Glu Ser Cys Glu Lys Val 
    370                 375                 380                 


Leu Gly Gln Lys Ala Trp Lys Leu Leu Trp Leu Lys Leu Glu Ser Lys 
385                 390                 395                 400 


Thr Leu Pro Lys Glu Val Pro Asp Met Gly Trp Ala Tyr Lys Asn Leu 
                405                 410                 415     


Ala Lys Leu Gly Gly Trp Lys Asp Thr Lys Arg Thr Gly Arg Ala Ser 
            420                 425                 430         


Ile Lys Val Leu Trp Glu Gly Trp Phe Lys Leu Gln Thr Ile Leu Glu 
        435                 440                 445             


Gly Tyr Glu Leu Ala Met Ser Leu Asp His 
    450                 455             


<210>  11
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  V. harveyi Transposase Recognition Sequence

<400>  11
ctgtctcttg atcacaagt                                                    19


<210>  12
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  V. harveyi Transposase Recognition Sequence

<400>  12
agatgtgatc aagagacag                                                    19


<210>  13
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  V. harveyi Transposase Recognition Sequence

<400>  13
ctgtctcttg atcacatct                                                    19


