                         SEQUENCE LISTING

<110>  New England Biolabs, Inc.
 
<120>  FCE MRNA CAPPING ENZYME COMPOSITIONS, METHODS AND KITS

<130>  NEB-438-PCT

<160>  28    

<170>  PatentIn version 3.5

<210>  1
<211>  886
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  FCE (WT) - 8His polypeptide

<220>
<221>  MISC_FEATURE
<222>  (215)..(215)
<223>  asparagine-linked glycosylation site

<220>
<221>  MISC_FEATURE
<222>  (337)..(337)
<223>  asparagine-linked glycosylation site

<220>
<221>  MISC_FEATURE
<222>  (572)..(572)
<223>  asparagine-linked glycosylation site

<220>
<221>  MISC_FEATURE
<222>  (648)..(648)
<223>  asparagine-linked glycosylation site

<220>
<221>  MISC_FEATURE
<222>  (833)..(833)
<223>  asparagine-linked glycosylation site

<400>  1

Ala Lys Arg Leu Gln Arg Cys Gln Asp Val Asn Gln Val Cys Glu Ile 
1               5                   10                  15      


Tyr Asn Ser Lys Gly Gly Ile Gly Glu Leu Glu Leu Arg Phe Asp Lys 
            20                  25                  30          


Leu Pro Gln Asn Leu Phe Ala Gly Val Phe Asp Lys Leu Lys Pro Asp 
        35                  40                  45              


Gly Glu Ile Gln Thr Thr Met Arg Val Ser Asn Arg Asp Gly Val Ala 
    50                  55                  60                  


Arg Glu Ile Thr Phe Gly Gly Gly Val Lys Thr Asn Glu Ile Phe Val 
65                  70                  75                  80  


Lys Lys Gln Asn Ile Cys Val Phe Asp Val Val Asp Ile Phe Ser Tyr 
                85                  90                  95      


Lys Val Ala Val Ser Thr Glu Glu Thr Val Val Glu Lys Pro Thr Met 
            100                 105                 110         


Glu Thr Thr Ala Gly Val Arg Phe Lys Ile Arg Leu Ser Val Glu Asp 
        115                 120                 125             


Val Val Lys Asp Trp Arg Ile Asp Leu Thr Ala Val Lys Thr Ala Glu 
    130                 135                 140                 


Leu Gly Lys Ile Ala Gln His Thr Ala Ser Ile Val Gln Arg Thr Phe 
145                 150                 155                 160 


Pro Asp Asn Leu Leu Lys Leu Thr Gly Ala Glu Val Ala Lys Leu Ala 
                165                 170                 175     


Ala Asp Ser Tyr Glu Leu Glu Leu Glu Tyr Thr Gly Lys Ser Pro Ala 
            180                 185                 190         


Thr Asn Glu Lys Val Asn Val Ala Ala Lys Tyr Ala Val Glu Leu Leu 
        195                 200                 205             


Ser Ser Val Arg Asn Ala Asn Ser Thr Ala Ala Ala Ser Phe Gly Glu 
    210                 215                 220                 


Ser Val Ser Asp Leu Cys Arg Val Ala Lys Ile Ile His Thr His Glu 
225                 230                 235                 240 


Tyr Ala Asn Val Val Cys Arg Thr Pro Ser Phe Lys Met Leu Leu Pro 
                245                 250                 255     


Gln Val Val Ser Leu Thr Lys Ser Ser Tyr Tyr Gly Gly Leu Tyr Pro 
            260                 265                 270         


Pro Glu Asn Leu Trp Leu Ala Gly Lys Thr Asp Gly Val Arg Ala Leu 
        275                 280                 285             


Val Val Cys Glu Asp Gly Val Ala Lys Val Ile Thr Ala Glu Ser Val 
    290                 295                 300                 


Asp Ile Thr His Gly Val Cys Ser Ala Thr Thr Ile Leu Asp Cys Glu 
305                 310                 315                 320 


Leu Asn Val Asp Ala Lys Ile Leu Tyr Val Phe Asp Val Ile Ile Ser 
                325                 330                 335     


Asn Asn Thr Gln Val Tyr Thr Gln Pro Phe Ser Thr Arg Ile Thr Thr 
            340                 345                 350         


Asp Ile Ser Asp Ile Lys Ile Asp Gly Tyr Lys Ile Glu Met Lys Pro 
        355                 360                 365             


Phe Val Lys Val Val Lys Ala Asp Glu Ala Thr Phe Lys Ser Ala Tyr 
    370                 375                 380                 


Lys Ala Pro His Asn Glu Gly Leu Ile Met Ile Glu Asp Gly Ala Ala 
385                 390                 395                 400 


Tyr Ala Ala Thr Lys Thr Tyr Lys Trp Lys Pro Leu Ser His Asn Thr 
                405                 410                 415     


Ile Asp Phe Leu Ile Lys Ala Cys Pro Lys Gln Leu Ile Asn Val Asp 
            420                 425                 430         


Pro Tyr Lys Pro Arg Ala Gly Tyr Lys Leu Trp Leu Leu Phe Thr Thr 
        435                 440                 445             


Ile Ser Leu Asp Gln Gln Arg Glu Leu Gly Ile Glu Phe Ile Pro Ala 
    450                 455                 460                 


Trp Lys Ile Leu Phe Thr Asp Ile Asn Met Phe Gly Ser Arg Val Pro 
465                 470                 475                 480 


Ile Gln Phe Gln Pro Ala Ile Asn Pro Leu Ala Tyr Val Cys Tyr Leu 
                485                 490                 495     


Pro Glu Asp Val Asn Val Asn Asp Gly Asp Ile Val Glu Met Arg Ala 
            500                 505                 510         


Val Asp Gly Tyr Asp Thr Ile Pro Lys Trp Glu Leu Val Arg Ser Arg 
        515                 520                 525             


Asn Asp Arg Lys Asn Glu Pro Gly Phe Tyr Gly Asn Asn Tyr Lys Ile 
    530                 535                 540                 


Ala Ser Asp Ile Tyr Leu Asn Tyr Ile Asp Val Phe His Phe Glu Asp 
545                 550                 555                 560 


Leu Tyr Lys Tyr Asn Pro Gly Tyr Phe Glu Lys Asn Lys Ser Asp Ile 
                565                 570                 575     


Tyr Val Ala Pro Asn Lys Tyr Arg Arg Tyr Leu Ile Lys Ser Leu Phe 
            580                 585                 590         


Gly Arg Tyr Leu Arg Asp Ala Lys Trp Val Ile Asp Ala Ala Ala Gly 
        595                 600                 605             


Arg Gly Ala Asp Leu His Leu Tyr Lys Ala Glu Cys Val Glu His Leu 
    610                 615                 620                 


Leu Ala Ile Asp Ile Asp Pro Thr Ala Ile Ser Glu Leu Val Arg Arg 
625                 630                 635                 640 


Arg Asn Glu Ile Thr Gly Tyr Asn Lys Ser His Arg Gly Gly Arg Asn 
                645                 650                 655     


Met His Ser His Arg Gly Gln Ser His Cys Ala Lys Ser Thr Ser Leu 
            660                 665                 670         


His Ala Leu Val Ala Asp Leu Arg Glu Asn Pro Asp Val Leu Ile Pro 
        675                 680                 685             


Lys Ile Ile Gln Ser Arg Pro His Glu Arg Cys Tyr Asp Ala Ile Val 
    690                 695                 700                 


Ile Asn Phe Ala Ile His Tyr Leu Cys Asp Thr Asp Glu His Ile Arg 
705                 710                 715                 720 


Asp Phe Leu Ile Thr Val Ser Arg Leu Leu Ala Pro Asn Gly Val Phe 
                725                 730                 735     


Ile Phe Thr Thr Met Asp Gly Glu Ser Ile Val Lys Leu Leu Ala Asp 
            740                 745                 750         


His Lys Val Arg Pro Gly Glu Ala Trp Thr Ile His Thr Gly Asp Val 
        755                 760                 765             


Asn Ser Pro Asp Ser Thr Val Pro Lys Tyr Ser Ile Arg Arg Leu Tyr 
    770                 775                 780                 


Asp Ser Asp Lys Leu Thr Lys Thr Gly Gln Gln Ile Glu Val Leu Leu 
785                 790                 795                 800 


Pro Met Ser Gly Glu Met Lys Ala Glu Pro Leu Cys Asn Ile Lys Asn 
                805                 810                 815     


Ile Ile Ser Met Ala Arg Lys Met Gly Leu Asp Leu Val Glu Ser Ala 
            820                 825                 830         


Asn Phe Ser Val Leu Tyr Glu Ala Tyr Ala Arg Asp Tyr Pro Asp Ile 
        835                 840                 845             


Tyr Ala Arg Met Thr Pro Asp Asp Lys Leu Tyr Asn Asp Leu His Thr 
    850                 855                 860                 


Tyr Ala Val Phe Lys Arg Lys Lys Gly Ala Ser Ala Thr Ser His His 
865                 870                 875                 880 


His His His His His His 
                885     


<210>  2
<211>  1274
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  MBP-FCE-8His polypeptide

<220>
<221>  MISC_FEATURE
<222>  (1)..(388)
<223>  maltose binding protein (MBP) (367 aa) + Linker + Enterokinase 
       (EK) cleavage sequence (DDDK)

<220>
<221>  MISC_FEATURE
<222>  (389)..(1274)
<223>  FCE mRNA Capping enzyme (878 aa) + 8xHis-tag

<220>
<221>  MISC_FEATURE
<222>  (603)..(603)
<223>  asparagine-linked glycosylation site

<220>
<221>  MISC_FEATURE
<222>  (725)..(725)
<223>  asparagine-linked glycosylation site

<220>
<221>  MISC_FEATURE
<222>  (960)..(960)
<223>  asparagine-linked glycosylation site

<220>
<221>  MISC_FEATURE
<222>  (1036)..(1036)
<223>  asparagine-linked glycosylation site

<220>
<221>  MISC_FEATURE
<222>  (1221)..(1221)
<223>  asparagine-linked glycosylation site

<400>  2

Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys 
1               5                   10                  15      


Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr 
            20                  25                  30          


Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe 
        35                  40                  45              


Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala 
    50                  55                  60                  


His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile 
65                  70                  75                  80  


Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp 
                85                  90                  95      


Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu 
            100                 105                 110         


Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys 
        115                 120                 125             


Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly 
    130                 135                 140                 


Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 
145                 150                 155                 160 


Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys 
                165                 170                 175     


Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly 
            180                 185                 190         


Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp 
        195                 200                 205             


Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala 
    210                 215                 220                 


Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 
225                 230                 235                 240 


Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser 
                245                 250                 255     


Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro 
            260                 265                 270         


Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp 
        275                 280                 285             


Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala 
    290                 295                 300                 


Leu Lys Ser Tyr Glu Glu Glu Leu Val Lys Asp Pro Arg Ile Ala Ala 
305                 310                 315                 320 


Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln 
                325                 330                 335     


Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala 
            340                 345                 350         


Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn 
        355                 360                 365             


Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Asp 
    370                 375                 380                 


Asp Asp Asp Lys Ala Lys Arg Leu Gln Arg Cys Gln Asp Val Asn Gln 
385                 390                 395                 400 


Val Cys Glu Ile Tyr Asn Ser Lys Gly Gly Ile Gly Glu Leu Glu Leu 
                405                 410                 415     


Arg Phe Asp Lys Leu Pro Gln Asn Leu Phe Ala Gly Val Phe Asp Lys 
            420                 425                 430         


Leu Lys Pro Asp Gly Glu Ile Gln Thr Thr Met Arg Val Ser Asn Arg 
        435                 440                 445             


Asp Gly Val Ala Arg Glu Ile Thr Phe Gly Gly Gly Val Lys Thr Asn 
    450                 455                 460                 


Glu Ile Phe Val Lys Lys Gln Asn Ile Cys Val Phe Asp Val Val Asp 
465                 470                 475                 480 


Ile Phe Ser Tyr Lys Val Ala Val Ser Thr Glu Glu Thr Val Val Glu 
                485                 490                 495     


Lys Pro Thr Met Glu Thr Thr Ala Gly Val Arg Phe Lys Ile Arg Leu 
            500                 505                 510         


Ser Val Glu Asp Val Val Lys Asp Trp Arg Ile Asp Leu Thr Ala Val 
        515                 520                 525             


Lys Thr Ala Glu Leu Gly Lys Ile Ala Gln His Thr Ala Ser Ile Val 
    530                 535                 540                 


Gln Arg Thr Phe Pro Asp Asn Leu Leu Lys Leu Thr Gly Ala Glu Val 
545                 550                 555                 560 


Ala Lys Leu Ala Ala Asp Ser Tyr Glu Leu Glu Leu Glu Tyr Thr Gly 
                565                 570                 575     


Lys Ser Pro Ala Thr Asn Glu Lys Val Asn Val Ala Ala Lys Tyr Ala 
            580                 585                 590         


Val Glu Leu Leu Ser Ser Val Arg Asn Ala Asn Ser Thr Ala Ala Ala 
        595                 600                 605             


Ser Phe Gly Glu Ser Val Ser Asp Leu Cys Arg Val Ala Lys Ile Ile 
    610                 615                 620                 


His Thr His Glu Tyr Ala Asn Val Val Cys Arg Thr Pro Ser Phe Lys 
625                 630                 635                 640 


Met Leu Leu Pro Gln Val Val Ser Leu Thr Lys Ser Ser Tyr Tyr Gly 
                645                 650                 655     


Gly Leu Tyr Pro Pro Glu Asn Leu Trp Leu Ala Gly Lys Thr Asp Gly 
            660                 665                 670         


Val Arg Ala Leu Val Val Cys Glu Asp Gly Val Ala Lys Val Ile Thr 
        675                 680                 685             


Ala Glu Ser Val Asp Ile Thr His Gly Val Cys Ser Ala Thr Thr Ile 
    690                 695                 700                 


Leu Asp Cys Glu Leu Asn Val Asp Ala Lys Ile Leu Tyr Val Phe Asp 
705                 710                 715                 720 


Val Ile Ile Ser Asn Asn Thr Gln Val Tyr Thr Gln Pro Phe Ser Thr 
                725                 730                 735     


Arg Ile Thr Thr Asp Ile Ser Asp Ile Lys Ile Asp Gly Tyr Lys Ile 
            740                 745                 750         


Glu Met Lys Pro Phe Val Lys Val Val Lys Ala Asp Glu Ala Thr Phe 
        755                 760                 765             


Lys Ser Ala Tyr Lys Ala Pro His Asn Glu Gly Leu Ile Met Ile Glu 
    770                 775                 780                 


Asp Gly Ala Ala Tyr Ala Ala Thr Lys Thr Tyr Lys Trp Lys Pro Leu 
785                 790                 795                 800 


Ser His Asn Thr Ile Asp Phe Leu Ile Lys Ala Cys Pro Lys Gln Leu 
                805                 810                 815     


Ile Asn Val Asp Pro Tyr Lys Pro Arg Ala Gly Tyr Lys Leu Trp Leu 
            820                 825                 830         


Leu Phe Thr Thr Ile Ser Leu Asp Gln Gln Arg Glu Leu Gly Ile Glu 
        835                 840                 845             


Phe Ile Pro Ala Trp Lys Ile Leu Phe Thr Asp Ile Asn Met Phe Gly 
    850                 855                 860                 


Ser Arg Val Pro Ile Gln Phe Gln Pro Ala Ile Asn Pro Leu Ala Tyr 
865                 870                 875                 880 


Val Cys Tyr Leu Pro Glu Asp Val Asn Val Asn Asp Gly Asp Ile Val 
                885                 890                 895     


Glu Met Arg Ala Val Asp Gly Tyr Asp Thr Ile Pro Lys Trp Glu Leu 
            900                 905                 910         


Val Arg Ser Arg Asn Asp Arg Lys Asn Glu Pro Gly Phe Tyr Gly Asn 
        915                 920                 925             


Asn Tyr Lys Ile Ala Ser Asp Ile Tyr Leu Asn Tyr Ile Asp Val Phe 
    930                 935                 940                 


His Phe Glu Asp Leu Tyr Lys Tyr Asn Pro Gly Tyr Phe Glu Lys Asn 
945                 950                 955                 960 


Lys Ser Asp Ile Tyr Val Ala Pro Asn Lys Tyr Arg Arg Tyr Leu Ile 
                965                 970                 975     


Lys Ser Leu Phe Gly Arg Tyr Leu Arg Asp Ala Lys Trp Val Ile Asp 
            980                 985                 990         


Ala Ala Ala Gly Arg Gly Ala Asp  Leu His Leu Tyr Lys  Ala Glu Cys 
        995                 1000                 1005             


Val Glu  His Leu Leu Ala Ile  Asp Ile Asp Pro Thr  Ala Ile Ser 
    1010                 1015                 1020             


Glu Leu  Val Arg Arg Arg Asn  Glu Ile Thr Gly Tyr  Asn Lys Ser 
    1025                 1030                 1035             


His Arg  Gly Gly Arg Asn Met  His Ser His Arg Gly  Gln Ser His 
    1040                 1045                 1050             


Cys Ala  Lys Ser Thr Ser Leu  His Ala Leu Val Ala  Asp Leu Arg 
    1055                 1060                 1065             


Glu Asn  Pro Asp Val Leu Ile  Pro Lys Ile Ile Gln  Ser Arg Pro 
    1070                 1075                 1080             


His Glu  Arg Cys Tyr Asp Ala  Ile Val Ile Asn Phe  Ala Ile His 
    1085                 1090                 1095             


Tyr Leu  Cys Asp Thr Asp Glu  His Ile Arg Asp Phe  Leu Ile Thr 
    1100                 1105                 1110             


Val Ser  Arg Leu Leu Ala Pro  Asn Gly Val Phe Ile  Phe Thr Thr 
    1115                 1120                 1125             


Met Asp  Gly Glu Ser Ile Val  Lys Leu Leu Ala Asp  His Lys Val 
    1130                 1135                 1140             


Arg Pro  Gly Glu Ala Trp Thr  Ile His Thr Gly Asp  Val Asn Ser 
    1145                 1150                 1155             


Pro Asp  Ser Thr Val Pro Lys  Tyr Ser Ile Arg Arg  Leu Tyr Asp 
    1160                 1165                 1170             


Ser Asp  Lys Leu Thr Lys Thr  Gly Gln Gln Ile Glu  Val Leu Leu 
    1175                 1180                 1185             


Pro Met  Ser Gly Glu Met Lys  Ala Glu Pro Leu Cys  Asn Ile Lys 
    1190                 1195                 1200             


Asn Ile  Ile Ser Met Ala Arg  Lys Met Gly Leu Asp  Leu Val Glu 
    1205                 1210                 1215             


Ser Ala  Asn Phe Ser Val Leu  Tyr Glu Ala Tyr Ala  Arg Asp Tyr 
    1220                 1225                 1230             


Pro Asp  Ile Tyr Ala Arg Met  Thr Pro Asp Asp Lys  Leu Tyr Asn 
    1235                 1240                 1245             


Asp Leu  His Thr Tyr Ala Val  Phe Lys Arg Lys Lys  Gly Ala Ser 
    1250                 1255                 1260             


Ala Thr  Ser His His His His  His His His His 
    1265                 1270                 


<210>  3
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Forward primer for amplification of an FCE variant

<400>  3
agaaaagaga ggccgaagct gcgaagcgtc tgcagcgt                               38


<210>  4
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Reverse primer for amplification of an FCE variant

<400>  4
agaaaagaga ggccgaagct gcgaagcgtc tgcagcgt                               38


<210>  5
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Forward primer for amplification of the FCE vector fragment

<400>  5
aggggcggcc gctcaaga                                                     18


<210>  6
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Reverse primer for amplification of the FCE vector fragment

<400>  6
agcttcggcc tctcttttc                                                    19


<210>  7
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Forward primer N215Q- Fwd

<400>  7
tcgtaacgcg caaagcaccg cgg                                               23


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Reverse primer N215Q-Rev

<400>  8
acgctgctca gcagctccac                                                   20


<210>  9
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Forward primer N337Q-Fwd

<400>  9
tatcattagc caaaacaccc aggtttatac                                        30


<210>  10
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Reverse primer N337Q-Rev

<400>  10
acgtcgaaca cgtacaga                                                     18


<210>  11
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Forward primer N572Q-Fwd

<400>  11
cttcgagaag caaaaaagcg atatctatg                                         29


<210>  12
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Reverse primer N572Q-Rev

<400>  12
tagcccgggt tatacttg                                                     18


<210>  13
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Forward primer N648Q-Fwd

<400>  13
accggctacc aaaagagcca ccgtggcggt                                        30


<210>  14
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Reverse primer N648Q-Rev

<400>  14
gtggctcttt tggtagccgg taatctcgtt                                        30


<210>  15
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Forward primer N833Q-Fwd

<400>  15
gaaagcgcgc aattcagcgt gctgtacgag                                        30


<210>  16
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Reverse primer N833Q-Rev

<400>  16
cacgctgaat tgcgcgcttt caaccagatc                                        30


<210>  17
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Forward primer for amplification of an FCE variant

<400>  17
tcggggatga cgatgacaag gcgaagcgtc tgcagcgt                               38


<210>  18
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Reverse primer for amplification of an FCE variant

<400>  18
tcagcctctc ttttctcgag ttagtggtgg tggtggtgg                              39


<210>  19
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Forward primer for amplification of a pKLMF-EK vector fragment

<400>  19
ctcgagaaaa gagaggctga agct                                              24


<210>  20
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Reverse primer for amplification of a pKLMF-EK vector fragment

<400>  20
cttgtcatcg tcatccccga g                                                 21


<210>  21
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Forward primer for amplification of the FCE assembled linear 
       expression cassette

<400>  21
gatcgactca taaaatagta acc                                               23


<210>  22
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Reverse primer for amplification of the FCE assembled linear 
       expression cassette

<400>  22
ccgcggaaat ttaggaattt taaac                                             25


<210>  23
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Substrate RNA for in vitro capping reaction

<400>  23
guagaacuuc gucgaguacg cucaa                                             25


<210>  24
<211>  6494
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  PD912 vector

<220>
<221>  misc_feature
<222>  (1157)..(1639)
<223>  Pichia GAP promoter

<220>
<221>  misc_feature
<222>  (1650)..(1916)
<223>  DNA encoding alpha mating factor signal peptide

<220>
<221>  misc_feature
<222>  (1917)..(4577)
<223>  DNA encoding FCE mRNA Capping enzyme (878 aa) + 8xHis-tag

<220>
<221>  misc_feature
<222>  (2559)..(2561)
<223>  asparagine to glutamine variant codon

<220>
<221>  misc_feature
<222>  (2925)..(2927)
<223>  asparagine to glutamine variant codon

<220>
<221>  misc_feature
<222>  (3630)..(3632)
<223>  asparagine to glutamine variant codon

<400>  24
gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc       60

acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg      120

ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg      180

ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa      240

cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg      300

aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga      360

gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct      420

gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca      480

gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc      540

ctgcggtacc cagatccaat tcccgctttg actgcctgaa atctccatcg cctacaatga      600

tgacatttgg atttggttga ctcatgttgg tattgtgaaa tagacgcaga tcgggaacac      660

tgaaaaatac acagttatta ttcatttaaa taacatccaa agacgaaagg ttgaatgaaa      720

cctttttgcc atccgacatc cacaggtcca ttctcacaca taagtgccaa acgcaacagg      780

aggggataca ctagcagcag accgttgcaa acgcaggacc tccactcctc ttctcctcaa      840

cacccacttt tgccatcgaa aaaccagccc agttattggg cttgattgga gctcgctcat      900

tccaattcct tctattaggc tactaacacc atgactttat tagcctgtct atcctggccc      960

ccctggcgag gttcatgttt gtttatttcc gaatgcaaca agctccgcat tacacccgaa     1020

catcactcca gatgagggct ttctgagtgt ggggtcaaat agtttcatgt tccccaaatg     1080

gcccaaaact gacagtttaa acgctgtctt ggaacctaat atgacaaaag cgtgatctca     1140

tccaagatga actaaggatc cttttttgta gaaatgtctt ggtgtcctcg tccaatcagg     1200

tagccatctc tgaaatatct ggctccgttg caactccgaa cgacctgctg gcaacgtaaa     1260

attctccggg gtaaaactta aatgtggagt aatggaacca gaaacgtctc ttcccttctc     1320

tctccttcca ccgcccgtta ccgtccctag gaaattttac tctgctggag agcttcttct     1380

acggccccct tgcagcaatg ctcttcccag cattacgttg cgggtaaaac ggaggtcgtg     1440

tacccgacct agcagcccag ggatggaaaa gtcccggccg tcgctggcaa taatagcggg     1500

cggacgcatg tcatgagatt attggaaacc accagaatcg aatataaaag gcgaacacct     1560

ttcccaattt tggtttctcc tgacccaaag actttaaatt taatttattt gtccctattt     1620

caatcaattg aacaactatt tccgaaacga tgagattccc atctattttc accgctgtct     1680

tgttcgctgc ctcctctgca ttggctgccc ctgttaacac taccactgaa gacgagactg     1740

ctcaaattcc agctgaagca gttatcggtt actctgacct tgagggtgat ttcgacgtcg     1800

ctgttttgcc tttctctaac tccactaaca acggtttgtt gttcattaac accactatcg     1860

cttccattgc tgctaaggaa gagggtgtct ctctcgagaa aagagaggcc gaagctgcga     1920

agcgtctgca gcgttgccaa gatgtgaacc aggtttgcga aatctacaac agcaagggtg     1980

gcattggcga gctggaactg cgtttcgaca aactgccgca gaacctgttc gcgggcgtgt     2040

ttgataagct gaaaccggac ggcgagatcc aaaccaccat gcgtgtgagc aaccgtgacg     2100

gtgttgcgcg tgaaattacc ttcggtggcg gtgtgaaaac caacgagatc ttcgttaaga     2160

aacaaaacat ttgcgtgttc gacgtggttg atatctttag ctacaaggtg gcggttagca     2220

ccgaggaaac cgtggttgaa aaaccgacca tggagaccac cgcgggcgtt cgtttcaaaa     2280

tccgtctgag cgtggaagac gtggttaagg attggcgtat tgacctgacc gcggttaaga     2340

ccgcggagct gggtaaaatc gcgcagcaca ccgcgagcat tgtgcaacgt acctttccgg     2400

ataacctgct gaagctgacc ggtgcggaag tggcgaaact ggcggcggac agctacgagc     2460

tggaactgga gtataccggc aagagcccgg cgaccaacga aaaggtgaac gttgcggcga     2520

aatacgcggt ggagctgctg agcagcgttc gtaacgcgca aagcaccgcg gcggcgagct     2580

ttggtgaaag cgtgagcgac ctgtgccgtg ttgcgaaaat cattcacacc cacgagtacg     2640

cgaacgtggt ttgccgtacc ccgagcttta aaatgctgct gccgcaggtg gttagcctga     2700

ccaagagcag ctactatggc ggtctgtatc cgccggaaaa cctgtggctg gcgggcaaga     2760

ccgatggtgt tcgtgcgctg gttgtgtgcg aagacggcgt ggcgaaagtt atcaccgcgg     2820

agagcgtgga tattacccac ggtgtttgca gcgcgaccac catcctggat tgcgagctga     2880

acgtggacgc gaagattctg tacgtgttcg acgttatcat tagccaaaac acccaggttt     2940

atacccaacc gtttagcacc cgtatcacca ccgacattag cgatatcaag atcgatggtt     3000

acaagatcga aatgaagccg ttcgtgaagg tggttaaagc ggacgaggcg acctttaaga     3060

gcgcgtataa agcgccgcac aacgaaggcc tgatcatgat tgaggatggt gcggcgtacg     3120

cggcgaccaa gacctataag tggaaaccgc tgagccacaa caccatcgat ttcctgatta     3180

aggcgtgccc gaaacagctg atcaacgttg acccgtacaa gccgcgtgcg ggttataaac     3240

tgtggctgct gttcaccacc attagcctgg atcagcaacg tgaactgggc atcgagttta     3300

ttccggcgtg gaaaatcctg ttcaccgaca ttaacatgtt tggtagccgt gttccgatcc     3360

agttccaacc ggcgattaac ccgctggcgt acgtgtgcta tctgccggaa gacgtgaacg     3420

ttaacgacgg cgatatcgtg gagatgcgtg cggttgacgg ttacgatacc attccgaaat     3480

gggaactggt gcgtagccgt aacgatcgta agaacgagcc gggcttttac ggtaacaact     3540

ataaaatcgc gagcgacatt tacctgaact atatcgatgt gttccacttt gaagacctgt     3600

acaagtataa cccgggctac ttcgagaagc aaaaaagcga tatctatgtt gcgccgaaca     3660

agtaccgtcg ttatctgatt aaaagcctgt ttggtcgtta cctgcgtgat gcgaaatggg     3720

ttattgatgc ggcggcgggt cgtggtgcgg acctgcacct gtataaagcg gaatgcgtgg     3780

agcacctgct ggcgatcgac attgatccga ccgcgatcag cgaactggtt cgtcgtcgta     3840

acgagattac cggctacaac aagagccacc gtggcggtcg taacatgcac agccaccgtg     3900

gtcagagcca ctgcgcgaaa agcaccagcc tgcacgcgct ggttgcggat ctgcgtgaaa     3960

acccggacgt gctgatcccg aagatcattc aaagccgtcc gcacgagcgt tgctacgatg     4020

cgatcgtgat taacttcgcg attcactatc tgtgcgacac cgatgaacac atccgtgact     4080

ttctgattac cgttagccgt ctgctggcgc cgaacggtgt gttcatcttt accaccatgg     4140

atggtgaaag cattgttaag ctgctggcgg accacaaagt tcgtccgggt gaagcgtgga     4200

ccatccacac cggtgatgtt aacagcccgg acagcaccgt gccgaaatac agcatccgtc     4260

gtctgtatga cagcgataag ctgaccaaaa ccggccagca aattgaggtg ctgctgccga     4320

tgagcggtga aatgaaggcg gagccgctgt gcaacatcaa aaacatcatt agcatggcgc     4380

gtaagatggg cctggatctg gttgaaagcg cgaacttcag cgtgctgtac gaggcgtatg     4440

cgcgtgacta cccggatatc tatgcgcgta tgaccccgga cgataagctg tacaacgacc     4500

tgcacaccta tgcggttttt aagcgtaaga aaggtgcgag cgcgaccagc catcatcacc     4560

accaccacca ccactaaagg ttgaaggggc ggccgctcaa gaggatgtca gaatgccatt     4620

tgcctgagag atgcaggctt catttttgat acttttttat ttgtaaccta tatagtatag     4680

gatttttttt gtcattttgt ttcttctcgt acgagcttgc tcctgatcag cctatctcgc     4740

agcagatgaa tatcttgtgg taggggtttg ggaaaatcat tcgagtttga tgtttttctt     4800

ggtatttccc actcctcttc agagtacaga agattaagtg aaaccttcgt ttgtgcggat     4860

ccttcagtaa tgtcttgttt cttttgttgc agtggtgagc cattttgact tcgtgaaagt     4920

ttctttagaa tagttgtttc cagaggccaa acattccacc cgtagtaaag tgcaagcgta     4980

ggaagaccaa gactggcata aatcaggtat aagtgtcgag cactggcagg tgatcttctg     5040

aaagtttcta ctagcagata agatccagta gtcatgcata tggcaacaat gtaccgtgtg     5100

gatctaagaa cgcgtcctac taaccttcgc attcgttggt ccagtttgtt gttatcgatc     5160

aacgtgacaa ggttgtcgat tccgcgtaag catgcatacc caaggacgcc tgttgcaatt     5220

ccaagtgagc cagttccaac aatctttgta atattagagc acttcattgt gttgcgcttg     5280

aaagtaaaat gcgaacaaat taagagataa tctcgaaacc gcgacttcaa acgccaatat     5340

gatgtgcggc acacaataag cgttcatatc cgctgggtga ctttctcgct ttaaaaaatt     5400

atccgaaaaa attttctaga gtgttgttac tttatacttc cggctcgtat aatacgacaa     5460

ggtgtaagga ggactaaacc atggctaaac tcacctctgc tgttccagtc ctgactgctc     5520

gtgatgttgc tggtgctgtt gagttctgga ctgatagact cggtttctcc cgtgacttcg     5580

tagaggacga ctttgccggt gttgtacgtg acgacgttac cctgttcatc tccgcagttc     5640

aggaccaggt tgtgccagac aacactctgg catgggtatg ggttcgtggt ctggacgaac     5700

tgtacgctga gtggtctgag gtcgtgtcta ccaacttccg tgatgcatct ggtccagcta     5760

tgaccgagat cggtgaacag ccctggggtc gtgagtttgc actgcgtgat ccagctggta     5820

actgcgtgca tttcgtcgca gaagaacagg actaacaatt gacaccttac gattatttag     5880

agagtattta ttagttttat tgtatgtata cggatgtttt attatctatt tatgccctta     5940

tattctgtaa ctatccaaaa gtcctatctt atcaagccag caatctatgt ccgcgaacgt     6000

caactaaaaa taagcttttt atgctgttct ctcttttttt cccttcggta taattatacc     6060

ttgcatccac agattctcct gccaaatttt gcataatcct ttacaacatg gctatatggg     6120

agcacttagc gccctccaaa acccatattg cctacgcatg tataggtgtt ttttccacaa     6180

tattttctct gtgctctctt tttattaaag agaagctcta tatcggagaa gcttctgtgg     6240

ccgttatatt cggccttatc gtgggaccac attgcctgaa ttggtttgcc ccggaagatt     6300

ggggaaactt ggatctgatt accttagctg caggtaccac tgagcgtcag accccgtaga     6360

aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac     6420

aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt     6480

tccgaaggta actg                                                       6494


<210>  25
<211>  886
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  Fully processed mature FCE variant protein

<400>  25

Ala Lys Arg Leu Gln Arg Cys Gln Asp Val Asn Gln Val Cys Glu Ile 
1               5                   10                  15      


Tyr Asn Ser Lys Gly Gly Ile Gly Glu Leu Glu Leu Arg Phe Asp Lys 
            20                  25                  30          


Leu Pro Gln Asn Leu Phe Ala Gly Val Phe Asp Lys Leu Lys Pro Asp 
        35                  40                  45              


Gly Glu Ile Gln Thr Thr Met Arg Val Ser Asn Arg Asp Gly Val Ala 
    50                  55                  60                  


Arg Glu Ile Thr Phe Gly Gly Gly Val Lys Thr Asn Glu Ile Phe Val 
65                  70                  75                  80  


Lys Lys Gln Asn Ile Cys Val Phe Asp Val Val Asp Ile Phe Ser Tyr 
                85                  90                  95      


Lys Val Ala Val Ser Thr Glu Glu Thr Val Val Glu Lys Pro Thr Met 
            100                 105                 110         


Glu Thr Thr Ala Gly Val Arg Phe Lys Ile Arg Leu Ser Val Glu Asp 
        115                 120                 125             


Val Val Lys Asp Trp Arg Ile Asp Leu Thr Ala Val Lys Thr Ala Glu 
    130                 135                 140                 


Leu Gly Lys Ile Ala Gln His Thr Ala Ser Ile Val Gln Arg Thr Phe 
145                 150                 155                 160 


Pro Asp Asn Leu Leu Lys Leu Thr Gly Ala Glu Val Ala Lys Leu Ala 
                165                 170                 175     


Ala Asp Ser Tyr Glu Leu Glu Leu Glu Tyr Thr Gly Lys Ser Pro Ala 
            180                 185                 190         


Thr Asn Glu Lys Val Asn Val Ala Ala Lys Tyr Ala Val Glu Leu Leu 
        195                 200                 205             


Ser Ser Val Arg Asn Ala Gln Ser Thr Ala Ala Ala Ser Phe Gly Glu 
    210                 215                 220                 


Ser Val Ser Asp Leu Cys Arg Val Ala Lys Ile Ile His Thr His Glu 
225                 230                 235                 240 


Tyr Ala Asn Val Val Cys Arg Thr Pro Ser Phe Lys Met Leu Leu Pro 
                245                 250                 255     


Gln Val Val Ser Leu Thr Lys Ser Ser Tyr Tyr Gly Gly Leu Tyr Pro 
            260                 265                 270         


Pro Glu Asn Leu Trp Leu Ala Gly Lys Thr Asp Gly Val Arg Ala Leu 
        275                 280                 285             


Val Val Cys Glu Asp Gly Val Ala Lys Val Ile Thr Ala Glu Ser Val 
    290                 295                 300                 


Asp Ile Thr His Gly Val Cys Ser Ala Thr Thr Ile Leu Asp Cys Glu 
305                 310                 315                 320 


Leu Asn Val Asp Ala Lys Ile Leu Tyr Val Phe Asp Val Ile Ile Ser 
                325                 330                 335     


Gln Asn Thr Gln Val Tyr Thr Gln Pro Phe Ser Thr Arg Ile Thr Thr 
            340                 345                 350         


Asp Ile Ser Asp Ile Lys Ile Asp Gly Tyr Lys Ile Glu Met Lys Pro 
        355                 360                 365             


Phe Val Lys Val Val Lys Ala Asp Glu Ala Thr Phe Lys Ser Ala Tyr 
    370                 375                 380                 


Lys Ala Pro His Asn Glu Gly Leu Ile Met Ile Glu Asp Gly Ala Ala 
385                 390                 395                 400 


Tyr Ala Ala Thr Lys Thr Tyr Lys Trp Lys Pro Leu Ser His Asn Thr 
                405                 410                 415     


Ile Asp Phe Leu Ile Lys Ala Cys Pro Lys Gln Leu Ile Asn Val Asp 
            420                 425                 430         


Pro Tyr Lys Pro Arg Ala Gly Tyr Lys Leu Trp Leu Leu Phe Thr Thr 
        435                 440                 445             


Ile Ser Leu Asp Gln Gln Arg Glu Leu Gly Ile Glu Phe Ile Pro Ala 
    450                 455                 460                 


Trp Lys Ile Leu Phe Thr Asp Ile Asn Met Phe Gly Ser Arg Val Pro 
465                 470                 475                 480 


Ile Gln Phe Gln Pro Ala Ile Asn Pro Leu Ala Tyr Val Cys Tyr Leu 
                485                 490                 495     


Pro Glu Asp Val Asn Val Asn Asp Gly Asp Ile Val Glu Met Arg Ala 
            500                 505                 510         


Val Asp Gly Tyr Asp Thr Ile Pro Lys Trp Glu Leu Val Arg Ser Arg 
        515                 520                 525             


Asn Asp Arg Lys Asn Glu Pro Gly Phe Tyr Gly Asn Asn Tyr Lys Ile 
    530                 535                 540                 


Ala Ser Asp Ile Tyr Leu Asn Tyr Ile Asp Val Phe His Phe Glu Asp 
545                 550                 555                 560 


Leu Tyr Lys Tyr Asn Pro Gly Tyr Phe Glu Lys Gln Lys Ser Asp Ile 
                565                 570                 575     


Tyr Val Ala Pro Asn Lys Tyr Arg Arg Tyr Leu Ile Lys Ser Leu Phe 
            580                 585                 590         


Gly Arg Tyr Leu Arg Asp Ala Lys Trp Val Ile Asp Ala Ala Ala Gly 
        595                 600                 605             


Arg Gly Ala Asp Leu His Leu Tyr Lys Ala Glu Cys Val Glu His Leu 
    610                 615                 620                 


Leu Ala Ile Asp Ile Asp Pro Thr Ala Ile Ser Glu Leu Val Arg Arg 
625                 630                 635                 640 


Arg Asn Glu Ile Thr Gly Tyr Asn Lys Ser His Arg Gly Gly Arg Asn 
                645                 650                 655     


Met His Ser His Arg Gly Gln Ser His Cys Ala Lys Ser Thr Ser Leu 
            660                 665                 670         


His Ala Leu Val Ala Asp Leu Arg Glu Asn Pro Asp Val Leu Ile Pro 
        675                 680                 685             


Lys Ile Ile Gln Ser Arg Pro His Glu Arg Cys Tyr Asp Ala Ile Val 
    690                 695                 700                 


Ile Asn Phe Ala Ile His Tyr Leu Cys Asp Thr Asp Glu His Ile Arg 
705                 710                 715                 720 


Asp Phe Leu Ile Thr Val Ser Arg Leu Leu Ala Pro Asn Gly Val Phe 
                725                 730                 735     


Ile Phe Thr Thr Met Asp Gly Glu Ser Ile Val Lys Leu Leu Ala Asp 
            740                 745                 750         


His Lys Val Arg Pro Gly Glu Ala Trp Thr Ile His Thr Gly Asp Val 
        755                 760                 765             


Asn Ser Pro Asp Ser Thr Val Pro Lys Tyr Ser Ile Arg Arg Leu Tyr 
    770                 775                 780                 


Asp Ser Asp Lys Leu Thr Lys Thr Gly Gln Gln Ile Glu Val Leu Leu 
785                 790                 795                 800 


Pro Met Ser Gly Glu Met Lys Ala Glu Pro Leu Cys Asn Ile Lys Asn 
                805                 810                 815     


Ile Ile Ser Met Ala Arg Lys Met Gly Leu Asp Leu Val Glu Ser Ala 
            820                 825                 830         


Asn Phe Ser Val Leu Tyr Glu Ala Tyr Ala Arg Asp Tyr Pro Asp Ile 
        835                 840                 845             


Tyr Ala Arg Met Thr Pro Asp Asp Lys Leu Tyr Asn Asp Leu His Thr 
    850                 855                 860                 


Tyr Ala Val Phe Lys Arg Lys Lys Gly Ala Ser Ala Thr Ser His His 
865                 870                 875                 880 


His His His His His His 
                885     


<210>  26
<211>  6494
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  pD912 vector

<220>
<221>  misc_feature
<222>  (1157)..(1639)
<223>  Pichia GAP promoter

<220>
<221>  misc_feature
<222>  (1650)..(1916)
<223>  DNA encoding alpha mating factor signal peptide

<220>
<221>  misc_feature
<222>  (1917)..(4577)
<223>  DNA encoding FCE mRNA Capping enzyme (878 aa) + 8xHis-tag

<220>
<221>  misc_feature
<222>  (2559)..(2561)
<223>  asparagine to glutamine variant codon

<220>
<221>  misc_feature
<222>  (2925)..(2927)
<223>  asparagine to glutamine variant codon

<220>
<221>  misc_feature
<222>  (3630)..(3632)
<223>  asparagine to glutamine variant codon

<220>
<221>  misc_feature
<222>  (3858)..(3860)
<223>  asparagine to glutamine variant codon

<220>
<221>  misc_feature
<222>  (4413)..(4415)
<223>  asparagine to glutamine variant codon

<400>  26
gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc       60

acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg      120

ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg      180

ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa      240

cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg      300

aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga      360

gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct      420

gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca      480

gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc      540

ctgcggtacc cagatccaat tcccgctttg actgcctgaa atctccatcg cctacaatga      600

tgacatttgg atttggttga ctcatgttgg tattgtgaaa tagacgcaga tcgggaacac      660

tgaaaaatac acagttatta ttcatttaaa taacatccaa agacgaaagg ttgaatgaaa      720

cctttttgcc atccgacatc cacaggtcca ttctcacaca taagtgccaa acgcaacagg      780

aggggataca ctagcagcag accgttgcaa acgcaggacc tccactcctc ttctcctcaa      840

cacccacttt tgccatcgaa aaaccagccc agttattggg cttgattgga gctcgctcat      900

tccaattcct tctattaggc tactaacacc atgactttat tagcctgtct atcctggccc      960

ccctggcgag gttcatgttt gtttatttcc gaatgcaaca agctccgcat tacacccgaa     1020

catcactcca gatgagggct ttctgagtgt ggggtcaaat agtttcatgt tccccaaatg     1080

gcccaaaact gacagtttaa acgctgtctt ggaacctaat atgacaaaag cgtgatctca     1140

tccaagatga actaaggatc cttttttgta gaaatgtctt ggtgtcctcg tccaatcagg     1200

tagccatctc tgaaatatct ggctccgttg caactccgaa cgacctgctg gcaacgtaaa     1260

attctccggg gtaaaactta aatgtggagt aatggaacca gaaacgtctc ttcccttctc     1320

tctccttcca ccgcccgtta ccgtccctag gaaattttac tctgctggag agcttcttct     1380

acggccccct tgcagcaatg ctcttcccag cattacgttg cgggtaaaac ggaggtcgtg     1440

tacccgacct agcagcccag ggatggaaaa gtcccggccg tcgctggcaa taatagcggg     1500

cggacgcatg tcatgagatt attggaaacc accagaatcg aatataaaag gcgaacacct     1560

ttcccaattt tggtttctcc tgacccaaag actttaaatt taatttattt gtccctattt     1620

caatcaattg aacaactatt tccgaaacga tgagattccc atctattttc accgctgtct     1680

tgttcgctgc ctcctctgca ttggctgccc ctgttaacac taccactgaa gacgagactg     1740

ctcaaattcc agctgaagca gttatcggtt actctgacct tgagggtgat ttcgacgtcg     1800

ctgttttgcc tttctctaac tccactaaca acggtttgtt gttcattaac accactatcg     1860

cttccattgc tgctaaggaa gagggtgtct ctctcgagaa aagagaggcc gaagctgcga     1920

agcgtctgca gcgttgccaa gatgtgaacc aggtttgcga aatctacaac agcaagggtg     1980

gcattggcga gctggaactg cgtttcgaca aactgccgca gaacctgttc gcgggcgtgt     2040

ttgataagct gaaaccggac ggcgagatcc aaaccaccat gcgtgtgagc aaccgtgacg     2100

gtgttgcgcg tgaaattacc ttcggtggcg gtgtgaaaac caacgagatc ttcgttaaga     2160

aacaaaacat ttgcgtgttc gacgtggttg atatctttag ctacaaggtg gcggttagca     2220

ccgaggaaac cgtggttgaa aaaccgacca tggagaccac cgcgggcgtt cgtttcaaaa     2280

tccgtctgag cgtggaagac gtggttaagg attggcgtat tgacctgacc gcggttaaga     2340

ccgcggagct gggtaaaatc gcgcagcaca ccgcgagcat tgtgcaacgt acctttccgg     2400

ataacctgct gaagctgacc ggtgcggaag tggcgaaact ggcggcggac agctacgagc     2460

tggaactgga gtataccggc aagagcccgg cgaccaacga aaaggtgaac gttgcggcga     2520

aatacgcggt ggagctgctg agcagcgttc gtaacgcgca aagcaccgcg gcggcgagct     2580

ttggtgaaag cgtgagcgac ctgtgccgtg ttgcgaaaat cattcacacc cacgagtacg     2640

cgaacgtggt ttgccgtacc ccgagcttta aaatgctgct gccgcaggtg gttagcctga     2700

ccaagagcag ctactatggc ggtctgtatc cgccggaaaa cctgtggctg gcgggcaaga     2760

ccgatggtgt tcgtgcgctg gttgtgtgcg aagacggcgt ggcgaaagtt atcaccgcgg     2820

agagcgtgga tattacccac ggtgtttgca gcgcgaccac catcctggat tgcgagctga     2880

acgtggacgc gaagattctg tacgtgttcg acgttatcat tagccaaaac acccaggttt     2940

atacccaacc gtttagcacc cgtatcacca ccgacattag cgatatcaag atcgatggtt     3000

acaagatcga aatgaagccg ttcgtgaagg tggttaaagc ggacgaggcg acctttaaga     3060

gcgcgtataa agcgccgcac aacgaaggcc tgatcatgat tgaggatggt gcggcgtacg     3120

cggcgaccaa gacctataag tggaaaccgc tgagccacaa caccatcgat ttcctgatta     3180

aggcgtgccc gaaacagctg atcaacgttg acccgtacaa gccgcgtgcg ggttataaac     3240

tgtggctgct gttcaccacc attagcctgg atcagcaacg tgaactgggc atcgagttta     3300

ttccggcgtg gaaaatcctg ttcaccgaca ttaacatgtt tggtagccgt gttccgatcc     3360

agttccaacc ggcgattaac ccgctggcgt acgtgtgcta tctgccggaa gacgtgaacg     3420

ttaacgacgg cgatatcgtg gagatgcgtg cggttgacgg ttacgatacc attccgaaat     3480

gggaactggt gcgtagccgt aacgatcgta agaacgagcc gggcttttac ggtaacaact     3540

ataaaatcgc gagcgacatt tacctgaact atatcgatgt gttccacttt gaagacctgt     3600

acaagtataa cccgggctac ttcgagaagc aaaaaagcga tatctatgtt gcgccgaaca     3660

agtaccgtcg ttatctgatt aaaagcctgt ttggtcgtta cctgcgtgat gcgaaatggg     3720

ttattgatgc ggcggcgggt cgtggtgcgg acctgcacct gtataaagcg gaatgcgtgg     3780

agcacctgct ggcgatcgac attgatccga ccgcgatcag cgaactggtt cgtcgtcgta     3840

acgagattac cggctaccaa aagagccacc gtggcggtcg taacatgcac agccaccgtg     3900

gtcagagcca ctgcgcgaaa agcaccagcc tgcacgcgct ggttgcggat ctgcgtgaaa     3960

acccggacgt gctgatcccg aagatcattc aaagccgtcc gcacgagcgt tgctacgatg     4020

cgatcgtgat taacttcgcg attcactatc tgtgcgacac cgatgaacac atccgtgact     4080

ttctgattac cgttagccgt ctgctggcgc cgaacggtgt gttcatcttt accaccatgg     4140

atggtgaaag cattgttaag ctgctggcgg accacaaagt tcgtccgggt gaagcgtgga     4200

ccatccacac cggtgatgtt aacagcccgg acagcaccgt gccgaaatac agcatccgtc     4260

gtctgtatga cagcgataag ctgaccaaaa ccggccagca aattgaggtg ctgctgccga     4320

tgagcggtga aatgaaggcg gagccgctgt gcaacatcaa aaacatcatt agcatggcgc     4380

gtaagatggg cctggatctg gttgaaagcg cgcaattcag cgtgctgtac gaggcgtatg     4440

cgcgtgacta cccggatatc tatgcgcgta tgaccccgga cgataagctg tacaacgacc     4500

tgcacaccta tgcggttttt aagcgtaaga aaggtgcgag cgcgaccagc catcatcacc     4560

accaccacca ccactaaagg ttgaaggggc ggccgctcaa gaggatgtca gaatgccatt     4620

tgcctgagag atgcaggctt catttttgat acttttttat ttgtaaccta tatagtatag     4680

gatttttttt gtcattttgt ttcttctcgt acgagcttgc tcctgatcag cctatctcgc     4740

agcagatgaa tatcttgtgg taggggtttg ggaaaatcat tcgagtttga tgtttttctt     4800

ggtatttccc actcctcttc agagtacaga agattaagtg aaaccttcgt ttgtgcggat     4860

ccttcagtaa tgtcttgttt cttttgttgc agtggtgagc cattttgact tcgtgaaagt     4920

ttctttagaa tagttgtttc cagaggccaa acattccacc cgtagtaaag tgcaagcgta     4980

ggaagaccaa gactggcata aatcaggtat aagtgtcgag cactggcagg tgatcttctg     5040

aaagtttcta ctagcagata agatccagta gtcatgcata tggcaacaat gtaccgtgtg     5100

gatctaagaa cgcgtcctac taaccttcgc attcgttggt ccagtttgtt gttatcgatc     5160

aacgtgacaa ggttgtcgat tccgcgtaag catgcatacc caaggacgcc tgttgcaatt     5220

ccaagtgagc cagttccaac aatctttgta atattagagc acttcattgt gttgcgcttg     5280

aaagtaaaat gcgaacaaat taagagataa tctcgaaacc gcgacttcaa acgccaatat     5340

gatgtgcggc acacaataag cgttcatatc cgctgggtga ctttctcgct ttaaaaaatt     5400

atccgaaaaa attttctaga gtgttgttac tttatacttc cggctcgtat aatacgacaa     5460

ggtgtaagga ggactaaacc atggctaaac tcacctctgc tgttccagtc ctgactgctc     5520

gtgatgttgc tggtgctgtt gagttctgga ctgatagact cggtttctcc cgtgacttcg     5580

tagaggacga ctttgccggt gttgtacgtg acgacgttac cctgttcatc tccgcagttc     5640

aggaccaggt tgtgccagac aacactctgg catgggtatg ggttcgtggt ctggacgaac     5700

tgtacgctga gtggtctgag gtcgtgtcta ccaacttccg tgatgcatct ggtccagcta     5760

tgaccgagat cggtgaacag ccctggggtc gtgagtttgc actgcgtgat ccagctggta     5820

actgcgtgca tttcgtcgca gaagaacagg actaacaatt gacaccttac gattatttag     5880

agagtattta ttagttttat tgtatgtata cggatgtttt attatctatt tatgccctta     5940

tattctgtaa ctatccaaaa gtcctatctt atcaagccag caatctatgt ccgcgaacgt     6000

caactaaaaa taagcttttt atgctgttct ctcttttttt cccttcggta taattatacc     6060

ttgcatccac agattctcct gccaaatttt gcataatcct ttacaacatg gctatatggg     6120

agcacttagc gccctccaaa acccatattg cctacgcatg tataggtgtt ttttccacaa     6180

tattttctct gtgctctctt tttattaaag agaagctcta tatcggagaa gcttctgtgg     6240

ccgttatatt cggccttatc gtgggaccac attgcctgaa ttggtttgcc ccggaagatt     6300

ggggaaactt ggatctgatt accttagctg caggtaccac tgagcgtcag accccgtaga     6360

aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac     6420

aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt     6480

tccgaaggta actg                                                       6494


<210>  27
<211>  886
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  Fully processed mature FCE variant protein

<400>  27

Ala Lys Arg Leu Gln Arg Cys Gln Asp Val Asn Gln Val Cys Glu Ile 
1               5                   10                  15      


Tyr Asn Ser Lys Gly Gly Ile Gly Glu Leu Glu Leu Arg Phe Asp Lys 
            20                  25                  30          


Leu Pro Gln Asn Leu Phe Ala Gly Val Phe Asp Lys Leu Lys Pro Asp 
        35                  40                  45              


Gly Glu Ile Gln Thr Thr Met Arg Val Ser Asn Arg Asp Gly Val Ala 
    50                  55                  60                  


Arg Glu Ile Thr Phe Gly Gly Gly Val Lys Thr Asn Glu Ile Phe Val 
65                  70                  75                  80  


Lys Lys Gln Asn Ile Cys Val Phe Asp Val Val Asp Ile Phe Ser Tyr 
                85                  90                  95      


Lys Val Ala Val Ser Thr Glu Glu Thr Val Val Glu Lys Pro Thr Met 
            100                 105                 110         


Glu Thr Thr Ala Gly Val Arg Phe Lys Ile Arg Leu Ser Val Glu Asp 
        115                 120                 125             


Val Val Lys Asp Trp Arg Ile Asp Leu Thr Ala Val Lys Thr Ala Glu 
    130                 135                 140                 


Leu Gly Lys Ile Ala Gln His Thr Ala Ser Ile Val Gln Arg Thr Phe 
145                 150                 155                 160 


Pro Asp Asn Leu Leu Lys Leu Thr Gly Ala Glu Val Ala Lys Leu Ala 
                165                 170                 175     


Ala Asp Ser Tyr Glu Leu Glu Leu Glu Tyr Thr Gly Lys Ser Pro Ala 
            180                 185                 190         


Thr Asn Glu Lys Val Asn Val Ala Ala Lys Tyr Ala Val Glu Leu Leu 
        195                 200                 205             


Ser Ser Val Arg Asn Ala Gln Ser Thr Ala Ala Ala Ser Phe Gly Glu 
    210                 215                 220                 


Ser Val Ser Asp Leu Cys Arg Val Ala Lys Ile Ile His Thr His Glu 
225                 230                 235                 240 


Tyr Ala Asn Val Val Cys Arg Thr Pro Ser Phe Lys Met Leu Leu Pro 
                245                 250                 255     


Gln Val Val Ser Leu Thr Lys Ser Ser Tyr Tyr Gly Gly Leu Tyr Pro 
            260                 265                 270         


Pro Glu Asn Leu Trp Leu Ala Gly Lys Thr Asp Gly Val Arg Ala Leu 
        275                 280                 285             


Val Val Cys Glu Asp Gly Val Ala Lys Val Ile Thr Ala Glu Ser Val 
    290                 295                 300                 


Asp Ile Thr His Gly Val Cys Ser Ala Thr Thr Ile Leu Asp Cys Glu 
305                 310                 315                 320 


Leu Asn Val Asp Ala Lys Ile Leu Tyr Val Phe Asp Val Ile Ile Ser 
                325                 330                 335     


Gln Asn Thr Gln Val Tyr Thr Gln Pro Phe Ser Thr Arg Ile Thr Thr 
            340                 345                 350         


Asp Ile Ser Asp Ile Lys Ile Asp Gly Tyr Lys Ile Glu Met Lys Pro 
        355                 360                 365             


Phe Val Lys Val Val Lys Ala Asp Glu Ala Thr Phe Lys Ser Ala Tyr 
    370                 375                 380                 


Lys Ala Pro His Asn Glu Gly Leu Ile Met Ile Glu Asp Gly Ala Ala 
385                 390                 395                 400 


Tyr Ala Ala Thr Lys Thr Tyr Lys Trp Lys Pro Leu Ser His Asn Thr 
                405                 410                 415     


Ile Asp Phe Leu Ile Lys Ala Cys Pro Lys Gln Leu Ile Asn Val Asp 
            420                 425                 430         


Pro Tyr Lys Pro Arg Ala Gly Tyr Lys Leu Trp Leu Leu Phe Thr Thr 
        435                 440                 445             


Ile Ser Leu Asp Gln Gln Arg Glu Leu Gly Ile Glu Phe Ile Pro Ala 
    450                 455                 460                 


Trp Lys Ile Leu Phe Thr Asp Ile Asn Met Phe Gly Ser Arg Val Pro 
465                 470                 475                 480 


Ile Gln Phe Gln Pro Ala Ile Asn Pro Leu Ala Tyr Val Cys Tyr Leu 
                485                 490                 495     


Pro Glu Asp Val Asn Val Asn Asp Gly Asp Ile Val Glu Met Arg Ala 
            500                 505                 510         


Val Asp Gly Tyr Asp Thr Ile Pro Lys Trp Glu Leu Val Arg Ser Arg 
        515                 520                 525             


Asn Asp Arg Lys Asn Glu Pro Gly Phe Tyr Gly Asn Asn Tyr Lys Ile 
    530                 535                 540                 


Ala Ser Asp Ile Tyr Leu Asn Tyr Ile Asp Val Phe His Phe Glu Asp 
545                 550                 555                 560 


Leu Tyr Lys Tyr Asn Pro Gly Tyr Phe Glu Lys Gln Lys Ser Asp Ile 
                565                 570                 575     


Tyr Val Ala Pro Asn Lys Tyr Arg Arg Tyr Leu Ile Lys Ser Leu Phe 
            580                 585                 590         


Gly Arg Tyr Leu Arg Asp Ala Lys Trp Val Ile Asp Ala Ala Ala Gly 
        595                 600                 605             


Arg Gly Ala Asp Leu His Leu Tyr Lys Ala Glu Cys Val Glu His Leu 
    610                 615                 620                 


Leu Ala Ile Asp Ile Asp Pro Thr Ala Ile Ser Glu Leu Val Arg Arg 
625                 630                 635                 640 


Arg Asn Glu Ile Thr Gly Tyr Gln Lys Ser His Arg Gly Gly Arg Asn 
                645                 650                 655     


Met His Ser His Arg Gly Gln Ser His Cys Ala Lys Ser Thr Ser Leu 
            660                 665                 670         


His Ala Leu Val Ala Asp Leu Arg Glu Asn Pro Asp Val Leu Ile Pro 
        675                 680                 685             


Lys Ile Ile Gln Ser Arg Pro His Glu Arg Cys Tyr Asp Ala Ile Val 
    690                 695                 700                 


Ile Asn Phe Ala Ile His Tyr Leu Cys Asp Thr Asp Glu His Ile Arg 
705                 710                 715                 720 


Asp Phe Leu Ile Thr Val Ser Arg Leu Leu Ala Pro Asn Gly Val Phe 
                725                 730                 735     


Ile Phe Thr Thr Met Asp Gly Glu Ser Ile Val Lys Leu Leu Ala Asp 
            740                 745                 750         


His Lys Val Arg Pro Gly Glu Ala Trp Thr Ile His Thr Gly Asp Val 
        755                 760                 765             


Asn Ser Pro Asp Ser Thr Val Pro Lys Tyr Ser Ile Arg Arg Leu Tyr 
    770                 775                 780                 


Asp Ser Asp Lys Leu Thr Lys Thr Gly Gln Gln Ile Glu Val Leu Leu 
785                 790                 795                 800 


Pro Met Ser Gly Glu Met Lys Ala Glu Pro Leu Cys Asn Ile Lys Asn 
                805                 810                 815     


Ile Ile Ser Met Ala Arg Lys Met Gly Leu Asp Leu Val Glu Ser Ala 
            820                 825                 830         


Gln Phe Ser Val Leu Tyr Glu Ala Tyr Ala Arg Asp Tyr Pro Asp Ile 
        835                 840                 845             


Tyr Ala Arg Met Thr Pro Asp Asp Lys Leu Tyr Asn Asp Leu His Thr 
    850                 855                 860                 


Tyr Ala Val Phe Lys Arg Lys Lys Gly Ala Ser Ala Thr Ser His His 
865                 870                 875                 880 


His His His His His His 
                885     


<210>  28
<211>  878
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<222>  (215)..(215)
<223>  Xaa can be any amino acid and optionally any amino acid other 
       than asparagine

<220>
<221>  MISC_FEATURE
<222>  (337)..(337)
<223>  Xaa can be any amino acid and optionally any amino acid other 
       than asparagine

<220>
<221>  MISC_FEATURE
<222>  (572)..(572)
<223>  Xaa can be any amino acid and optionally any amino acid other 
       than asparagine

<220>
<221>  MISC_FEATURE
<222>  (648)..(648)
<223>  Xaa can be any amino acid and optionally any amino acid other 
       than asparagine

<220>
<221>  MISC_FEATURE
<222>  (833)..(833)
<223>  Xaa can be any amino acid and optionally any amino acid other 
       than asparagine

<400>  28

Ala Lys Arg Leu Gln Arg Cys Gln Asp Val Asn Gln Val Cys Glu Ile 
1               5                   10                  15      


Tyr Asn Ser Lys Gly Gly Ile Gly Glu Leu Glu Leu Arg Phe Asp Lys 
            20                  25                  30          


Leu Pro Gln Asn Leu Phe Ala Gly Val Phe Asp Lys Leu Lys Pro Asp 
        35                  40                  45              


Gly Glu Ile Gln Thr Thr Met Arg Val Ser Asn Arg Asp Gly Val Ala 
    50                  55                  60                  


Arg Glu Ile Thr Phe Gly Gly Gly Val Lys Thr Asn Glu Ile Phe Val 
65                  70                  75                  80  


Lys Lys Gln Asn Ile Cys Val Phe Asp Val Val Asp Ile Phe Ser Tyr 
                85                  90                  95      


Lys Val Ala Val Ser Thr Glu Glu Thr Val Val Glu Lys Pro Thr Met 
            100                 105                 110         


Glu Thr Thr Ala Gly Val Arg Phe Lys Ile Arg Leu Ser Val Glu Asp 
        115                 120                 125             


Val Val Lys Asp Trp Arg Ile Asp Leu Thr Ala Val Lys Thr Ala Glu 
    130                 135                 140                 


Leu Gly Lys Ile Ala Gln His Thr Ala Ser Ile Val Gln Arg Thr Phe 
145                 150                 155                 160 


Pro Asp Asn Leu Leu Lys Leu Thr Gly Ala Glu Val Ala Lys Leu Ala 
                165                 170                 175     


Ala Asp Ser Tyr Glu Leu Glu Leu Glu Tyr Thr Gly Lys Ser Pro Ala 
            180                 185                 190         


Thr Asn Glu Lys Val Asn Val Ala Ala Lys Tyr Ala Val Glu Leu Leu 
        195                 200                 205             


Ser Ser Val Arg Asn Ala Xaa Ser Thr Ala Ala Ala Ser Phe Gly Glu 
    210                 215                 220                 


Ser Val Ser Asp Leu Cys Arg Val Ala Lys Ile Ile His Thr His Glu 
225                 230                 235                 240 


Tyr Ala Asn Val Val Cys Arg Thr Pro Ser Phe Lys Met Leu Leu Pro 
                245                 250                 255     


Gln Val Val Ser Leu Thr Lys Ser Ser Tyr Tyr Gly Gly Leu Tyr Pro 
            260                 265                 270         


Pro Glu Asn Leu Trp Leu Ala Gly Lys Thr Asp Gly Val Arg Ala Leu 
        275                 280                 285             


Val Val Cys Glu Asp Gly Val Ala Lys Val Ile Thr Ala Glu Ser Val 
    290                 295                 300                 


Asp Ile Thr His Gly Val Cys Ser Ala Thr Thr Ile Leu Asp Cys Glu 
305                 310                 315                 320 


Leu Asn Val Asp Ala Lys Ile Leu Tyr Val Phe Asp Val Ile Ile Ser 
                325                 330                 335     


Xaa Asn Thr Gln Val Tyr Thr Gln Pro Phe Ser Thr Arg Ile Thr Thr 
            340                 345                 350         


Asp Ile Ser Asp Ile Lys Ile Asp Gly Tyr Lys Ile Glu Met Lys Pro 
        355                 360                 365             


Phe Val Lys Val Val Lys Ala Asp Glu Ala Thr Phe Lys Ser Ala Tyr 
    370                 375                 380                 


Lys Ala Pro His Asn Glu Gly Leu Ile Met Ile Glu Asp Gly Ala Ala 
385                 390                 395                 400 


Tyr Ala Ala Thr Lys Thr Tyr Lys Trp Lys Pro Leu Ser His Asn Thr 
                405                 410                 415     


Ile Asp Phe Leu Ile Lys Ala Cys Pro Lys Gln Leu Ile Asn Val Asp 
            420                 425                 430         


Pro Tyr Lys Pro Arg Ala Gly Tyr Lys Leu Trp Leu Leu Phe Thr Thr 
        435                 440                 445             


Ile Ser Leu Asp Gln Gln Arg Glu Leu Gly Ile Glu Phe Ile Pro Ala 
    450                 455                 460                 


Trp Lys Ile Leu Phe Thr Asp Ile Asn Met Phe Gly Ser Arg Val Pro 
465                 470                 475                 480 


Ile Gln Phe Gln Pro Ala Ile Asn Pro Leu Ala Tyr Val Cys Tyr Leu 
                485                 490                 495     


Pro Glu Asp Val Asn Val Asn Asp Gly Asp Ile Val Glu Met Arg Ala 
            500                 505                 510         


Val Asp Gly Tyr Asp Thr Ile Pro Lys Trp Glu Leu Val Arg Ser Arg 
        515                 520                 525             


Asn Asp Arg Lys Asn Glu Pro Gly Phe Tyr Gly Asn Asn Tyr Lys Ile 
    530                 535                 540                 


Ala Ser Asp Ile Tyr Leu Asn Tyr Ile Asp Val Phe His Phe Glu Asp 
545                 550                 555                 560 


Leu Tyr Lys Tyr Asn Pro Gly Tyr Phe Glu Lys Xaa Lys Ser Asp Ile 
                565                 570                 575     


Tyr Val Ala Pro Asn Lys Tyr Arg Arg Tyr Leu Ile Lys Ser Leu Phe 
            580                 585                 590         


Gly Arg Tyr Leu Arg Asp Ala Lys Trp Val Ile Asp Ala Ala Ala Gly 
        595                 600                 605             


Arg Gly Ala Asp Leu His Leu Tyr Lys Ala Glu Cys Val Glu His Leu 
    610                 615                 620                 


Leu Ala Ile Asp Ile Asp Pro Thr Ala Ile Ser Glu Leu Val Arg Arg 
625                 630                 635                 640 


Arg Asn Glu Ile Thr Gly Tyr Xaa Lys Ser His Arg Gly Gly Arg Asn 
                645                 650                 655     


Met His Ser His Arg Gly Gln Ser His Cys Ala Lys Ser Thr Ser Leu 
            660                 665                 670         


His Ala Leu Val Ala Asp Leu Arg Glu Asn Pro Asp Val Leu Ile Pro 
        675                 680                 685             


Lys Ile Ile Gln Ser Arg Pro His Glu Arg Cys Tyr Asp Ala Ile Val 
    690                 695                 700                 


Ile Asn Phe Ala Ile His Tyr Leu Cys Asp Thr Asp Glu His Ile Arg 
705                 710                 715                 720 


Asp Phe Leu Ile Thr Val Ser Arg Leu Leu Ala Pro Asn Gly Val Phe 
                725                 730                 735     


Ile Phe Thr Thr Met Asp Gly Glu Ser Ile Val Lys Leu Leu Ala Asp 
            740                 745                 750         


His Lys Val Arg Pro Gly Glu Ala Trp Thr Ile His Thr Gly Asp Val 
        755                 760                 765             


Asn Ser Pro Asp Ser Thr Val Pro Lys Tyr Ser Ile Arg Arg Leu Tyr 
    770                 775                 780                 


Asp Ser Asp Lys Leu Thr Lys Thr Gly Gln Gln Ile Glu Val Leu Leu 
785                 790                 795                 800 


Pro Met Ser Gly Glu Met Lys Ala Glu Pro Leu Cys Asn Ile Lys Asn 
                805                 810                 815     


Ile Ile Ser Met Ala Arg Lys Met Gly Leu Asp Leu Val Glu Ser Ala 
            820                 825                 830         


Xaa Phe Ser Val Leu Tyr Glu Ala Tyr Ala Arg Asp Tyr Pro Asp Ile 
        835                 840                 845             


Tyr Ala Arg Met Thr Pro Asp Asp Lys Leu Tyr Asn Asp Leu His Thr 
    850                 855                 860                 


Tyr Ala Val Phe Lys Arg Lys Lys Gly Ala Ser Ala Thr Ser 
865                 870                 875             


