                         SEQUENCE LISTING

<110>  BASF SE

<120>  High temperature fermentation process and microorganisms

<130>  PF Number PF202778

<160>  26

<170>  According Wipo Std 25


<210> 1
<211> 3519
<212> DNA
<213> Bacillus licheniformis

<220>
<223> Functional fragment: Bacillus licheniformis DSM13: proHJAA
      gene cluster with 5-prime regulatory region (data base
      accession CP000002)

<400> 1
gaatctgcct tcagcctcct tttcttttcc ttttttcttc aaaaatctcc atttttcaca     60
tgctctgcgc cgtccaagca aaacacgcaa acttgacata atgatctaaa gtgttaatat    120
actaaccgtg ttcgaaaaat acgaacagaa atgcgaacaa agggagatgt taaagatctt    180
taatcaaaaa aacgtagctt ttataggggc gggatcaatg gcagagggaa tgatctcagg    240
catcgttcaa tccaaaaaac tgcctgcttc gcaaatctat gttacaaacc acacgaacca    300
ccggcgttta gaagaactcc agcagaaata tggaatacaa ggcgtcaaaa aagaacaact    360
tgactatcag aacatcgatt gcttcatcct ggcgatgaag ccgaaagatg cagagggcgc    420
tcttaattcc atgaaaccgc atatcaaacc ccatcagctt atcgtgtcag ttttagctgg    480
catatcaata tcatttattg aaaacacgct gcacgacatg cagccggtca tcagaatcat    540
gcctaatacc tcaagtgcaa tcggcgcatc tgcaaccgcc atggccgaag gaagccatgt    600
aacggccgat ctgtcaaaaa ccgcgaaaca actgcttcaa tgcatgggag aagtctattc    660
catcgaagaa agcaaaatgg acattttcac tggcattgcc ggcagcgggc ctgcttactt    720
ttattattta atggagcaca tcgaaaaagc gggagccgag gctggattaa gccaaaaatt    780
aacaagggaa atcggcgcgc agacattgtt aggcgccgcc aaaatgctgt tggaaagccc    840
tgaggataca gctgttctca aagacaacat cacttcccct aacggtacga ctgccgccgg    900
tttaaaggcc ttggaagaaa gcggcggagg cgaagcgatc gaacaggcgg tcaaacaagc    960
cgcaaaacga tccaaaacga tcagtctcga actggaacaa ttagtcgcag caaagtaaat   1020
gcccgtaacg aagaaaaata ctacaggagt gataaaatga cctctaatca acagagggta   1080
gttgtcaaag tgggaagcag ttctttaaca agtcttcacg gcgaaatcag cagcaaaaag   1140
ctcgaaaagc tcgttgagca gattgtccgc ctcaaagatg aaggacacga agtcctcctt   1200
gtttcatctg gcgctgtagc agccggttac cggaagctcg gctttatcaa caggccggat   1260
acattgcctg aaaaacaagc atccgcttct gtcggccaag gcttattaat cgaagcgtat   1320
tccaagcttt ttttatccca cggctatatc gcttcacaaa ttttaatcac aaaaagcgat   1380
ttttcagatg aaaaacggta caacaatgtg agaaatacga tgaacgtgct gctgaaacgc   1440
ggcattattc cgatcatcaa cgaaaatgat acggttaccg tcaatcggct caaattcgga   1500
gacaatgaca cgctttctgc taaagtggcg ggtctgatcg acgccgacct gctgaccatc   1560
ctctctgata tagacggcct ttatgacgcg aatccgcgaa aaacgccgga cgccgcactc   1620
gtccaggaag taactgaaat cacgccggaa atagaagctt cagcagggga tgcaggaagc   1680
gaagtcggaa ccggcggcat gaagtccaaa ctggacgctt ttaaaattac aatggcttca   1740
ggcatcaaag ggtttttagg gcgcgcggac acccccggca ttttgcacga agcgatctgc   1800
gggactgcga ggggaacgta ttttataccg aatgcggatt cacacgccct caaccataaa   1860
aagcaatgga tagcatttaa ctctggacct gaaggagaca tcgtcgtcca caacgaaagc   1920
aaagagttta ttatcgaccg taaaaaaagc ctgtatcctt ctagtattta cgacatcaac   1980
ggccgtttca gcaacggatc cgtcgttcga atccttgatc aggaaggaga ggaaatcggc   2040
ctcggcgtcg tcaacttttc ctcatcccag cttcgtaaat ggaagaagga tacgaatcag   2100
gaaacagccg cttcctcaca ggctgtcgtt gacagagaag cgtttgtctg tcatttagag   2160
ctatccgttc cgctttaaaa aacaaatcaa aggaggaatt gacatgactg cgacgagcga   2220
gagcatcagt gcacaaatcg aagaaaaagc gataaaggca aaaacggccg caaaatcttt   2280
gcggctggca actgaaaaag aaaagaacga ggcgcttgct gcattggccg accacctgaa   2340
acaaaacatg agctatattc tcactgaaaa tgccaaagat atcgaagcgg gacgaaaaaa   2400
aggctatgat gcagcctaca tcgaccgcct gtcgctcaat gaagacaggg ttctggattt   2460
tgccgatggg ctgctggaag tcgcggaact tgatgatccg gtcggagaaa cgctatcaag   2520
ctggacgctc gacaacgggt taaaagcgga aaaagtcagc gttcctctcg gcgtcatcgg   2580
catgatctat gaagcgcgtc cgaacgtaac tgtcgatgcg accgggcttg ctctaaaatc   2640
aggcaatgcg atcgttttga aaggcggctc ttccgccatt cattcgaata cagcgatcgt   2700
atcggtcatg catcaagcgc ttgattcgac aaacatcccc cgcgatgccg ttcaattcat   2760
cgaaagcaca gaccgaagcg caacgaagca gctctttaag atgaaagaac atatcgatgt   2820
actcatcccc cggggaggcg gggccctcat tcaggaagtc gttgagaacg caaccgttcc   2880
ggtccttgaa acgggagtcg gcaactgcca catctatatt gaccgggaag cggaccctga   2940
aaaagcgatc gatgtcatga tcaatgcgaa aacagaccgc cccgccgtct gcaatgcact   3000
tgaaacgctg atcgtccacg aaaaatggct tgaggaaaac agcgccgcgc ttatcaatgc   3060
tttaaacgaa cacgggattc aagttcacgg ggacgaaaag gctgttcaaa tgattccagg   3120
cgcaagacca gcaggggaag ccgattggca gaacgaatat ttaagccttg atctcgcggt   3180
taaagtcgca ggaagtcttg aagaagcctg cgatcatatc gaaacatacg gaacaaagca   3240
ttcagaagcg attatcaccg aaaatccgga aacagccaaa gcttttctga ataccgttga   3300
tgctgcagca ttgtaccaca atgcctcaac caggttcacc gacggcggcg cactcggatt   3360
cggcgctgaa atcggaattt ctacacagaa gcttcacgcc cgcggaccta tgggtctgaa   3420
agaattgacc acatgcaaat atatcatgag aggcgacggc catatcagaa gataaataaa   3480
gagacctgta ctgaaactgc acctggtttg tcagacacc                          3519

<210> 2
<211> 217
<212> DNA
<213> Bacillus licheniformis

<220>
<223> Promoter: Bacillus licheniformis DSM13: 5-prime regulatory
      region of proHJAA operon (data base accession CP000002)

<400> 2
gaatctgcct tcagcctcct tttcttttcc ttttttcttc aaaaatctcc atttttcaca     60
tgctctgcgc cgtccaagca aaacacgcaa acttgacata atgatctaaa gtgttaatat    120
actaaccgtg ttcgaaaaat acgaacagaa atgcgaacaa agggagatgt taaagatctt    180
taatcaaaaa aacgtagctt ttataggggc gggatca                             217

<210> 3
<211> 39
<212> DNA
<213> Artificial sequence

<220>
<223> Oligonucleotide: PCR-amplification of proHJAA gene cluster
      Bacillus licheniformis

<400> 3
cataaatagg aggtaggtac gaatctgcct tcagcctcc                            39

<210> 4
<211> 35
<212> DNA
<213> Artificial sequence

<220>
<223> Oligonucleotide: PCR-amplification of proHJAA gene cluster
      Bacillus licheniformis

<400> 4
gaattcgagc tcatgggtgt ctgacaaacc aggtg                                35

<210> 5
<211> 36
<212> DNA
<213> Artificial sequence

<220>
<223> Oligonucleotide: PCR-amplification of proHJAA gene cluster
      Bacillus licheniformis

<400> 5
aatcaaaggg ggaaatggga atctgccttc agcctc                               36

<210> 6
<211> 35
<212> DNA
<213> Artificial sequence

<220>
<223> Oligonucleotide: PCR-amplification of proHJAA gene cluster
      Bacillus licheniformis

<400> 6
gaattcgagc tcatgggtgt ctgacaaacc aggtg                                35

<210> 7
<211> 46
<212> DNA
<213> Artificial sequence

<220>
<223> Oligonucleotide: PCR-amplification of the proBA region of
      Bacillus methanolicus

<400> 7
gctatgacca tgattacgaa ttccctcgaa tttgggtgtg aaacac                    46

<210> 8
<211> 50
<212> DNA
<213> Artificial sequence

<220>
<223> Oligonucleotide: PCR-amplification of the proBA region of
      Bacillus methanolicus

<400> 8
catttttgtt tcctcccata tccttaaatt atttgcgtat ttgaccattc                50

<210> 9
<211> 48
<212> DNA
<213> Artificial sequence

<220>
<223> Oligonucleotide: PCR-amplification of the proI region of
      Bacillus methanolicus

<400> 9
gtcaaatacg caaataattt aaggatatgg gaggaaacaa aaatgaag                  48

<210> 10
<211> 45
<212> DNA
<213> Artificial sequence

<220>
<223> Oligonucleotide: PCR-amplification of the proI region of
      Bacillus methanolicus

<400> 10
cttggctgca ggtcgacgga tcccccatgg gggacataac gattc                     45

<210> 11
<211> 487
<212> DNA
<213> Bacillus methanolicus

<220>
<223> Promoter: Bacillus methanolicus MGA3: proBA 5-prime
      promoter and gene regulatory region (data base
      accession CP007739)

<400> 11
gctatgacca tgattacgaa ttccctcgaa tttgggtgtg aaacacttcc attttagacg     60
aaaatgggta taattaagtg tgagtttttc aaaaaataaa ataattttct attgacagaa    120
acattgcttc ctttacataa taaaaaaaaa tgaacgttga cggaggatta gtatgcgggt    180
tctttgtgcg acagagagcg gggtttataa gctggaagac ttcgccgcat caaagccgca    240
gaacctgcct cctgagtcct gtgcaaaaac acgggcgctg acagagcgtt atcatgaaga    300
gcgggatgcg tatgcatcca ataaaggtgg taccacggaa atggaattgc agcccttttc    360
gtccttttct aaaggatgaa aagggctttt ttaatgaggt cccagagggc cagacccgct    420
cttcgttaat gtattaaagc gatatcaatc tcattcacgc atctttctgc aacaggagga    480
agtcgat                                                              487

<210> 12
<211> 23
<212> DNA
<213> Artificial sequence

<220>
<223> Oligonucleotide: Deletion of T-Box

<400> 12
gtaaaggaag caatgtttct gtc                                             23

<210> 13
<211> 19
<212> DNA
<213> Artificial sequence

<220>
<223> Oligonucleotide: Deletion of T-Box

<400> 13
agacccgctc ttcgttaat                                                  19

<210> 14
<211> 27
<212> DNA
<213> Artificial sequence

<220>
<223> Oligonucleotide

<400> 14
ccaaatgtca atctttctac tgaaacc                                         27

<210> 15
<211> 22
<212> DNA
<213> Artificial sequence

<220>
<223> Oligonucleotide

<400> 15
cgataatgat atgctttcgg cg                                              22

<210> 16
<211> 44
<212> DNA
<213> Artificial sequence

<220>
<223> Oligonucleotide

<400> 16
ataaatagga ggtaggtacc atggataaaa aacgaattgt cgtt                      44

<210> 17
<211> 40
<212> DNA
<213> Artificial sequence

<220>
<223> Oligonucleotide

<400> 17
gaattcgagc tcatggtacc catgggggac ataacgattc                           40

<210> 18
<211> 49
<212> DNA
<213> Artificial sequence

<220>
<223> Oligonucleotide

<400> 18
caaatacgca aataatttaa ggatatggga ggaaacaaaa atgaagaag                 49

<210> 19
<211> 45
<212> DNA
<213> Artificial sequence

<220>
<223> Oligonucleotide

<400> 19
gccagtgaat tcgagctcat ggtaccatgg gggacataac gattc                     45

<210> 20
<211> 1095
<212> DNA
<213> Bacillus methanolicus

<220>
<223> Bacillus methanolicus MGA3: proB gene (CDS)

<400> 20
atggataaaa aacgaattgt cgttaagatt ggaagcagtt cgttaaccaa ttcaaaagga     60
gaaattgacc acgagaagtt ttcagatcat attagagcca tagcagcttt gcgagcggag    120
ggccatgagg tcttaatcgt atcctccggt gcagttgcgg ccggatttaa acgccttggt    180
tatccgtcaa gacctgtaac gattaaaggg aagcaggctg cagctgcagt tggccagagc    240
ctactaatcc aagcctatat ggaaaagttc ggagaattcg gtatacttcc tgcgcaaatt    300
ttgctaacga gaaacgattt ttccaaaaaa gagcgttata gaaatgcata ctcaacaatt    360
atggagctat tggaacgcgg aattgtgccg attattaatg aaaacgatac ggtttcagta    420
gaagaattga catttggcga taatgatatg ctttcggcgc ttgtaagcgg aatggttcat    480
gccgatatgc tgattatttt aacggatatt aatggactat atgattcgaa tccaaagaaa    540
aacaggtttg ccaagaggat taacattcta catgagatca ctgatgaaat gatggctgct    600
gcagaaggga caggttccaa agtgggaacc ggaggcatgc tttcaaaatt aacggcggca    660
aaaacagccc tttctctcgg tgtaagagtt tttataggaa aaggaaaagg gagcagaaag    720
ctgctggata ttttggaagg cagaggcgat ggaacatatg tcgggaaaaa tgtcataccg    780
atcaagaata gtaaacaatg gattgctttt cactcagatg tttcaggaaa aatttatgta    840
gacaaaggag cggaggaagc acttctttct cacggaaaaa gccttctacc tgcaggaatt    900
tataaaataa caggtacgtt taaacgaggc gacgttgttg aagtgtttgg aacaaatggt    960
ttacttggaa aaggagaagt gctttattcc tcagaggaac tgaaaaaagt gatgggcaaa   1020
cgaagcgatg aattgaaaaa agatacagtt atggccattg aagtgattca ccgtgataag   1080
tgggtaaaag cataa                                                    1095

<210> 21
<211> 1248
<212> DNA
<213> Bacillus methanolicus

<220>
<223> Bacillus methanolicus MGA3: proA gene (CDS)

<400> 21
atgagtgaag tgaaaagaaa aggcaaagct gcaaaacaag taagctattc tttggtgaat     60
ttaactacag aggcaaaaaa tgaggcatta gaaaaaatcg cagttcaatt attaaaagat    120
aagaatttct tgcttcaaga aaaccaaaaa gatatcgagc atggaagacg tacagggttg    180
tcagatgcaa tactcgatag gattttgtta actgaaaagc ggattgaaga tatggcggga    240
gcaattaggc tgttaattgg tcttaaggat ccgatcggtg aaacattgga gaccattgaa    300
aaagaaaatg ggcttttgat tcaaaagaaa cgtgtaccga ttggtgtaat tggcatgata    360
tatgaagcac gtccgaatgt tacggttgat gcggcgacgt tatcgcttaa aacaggaaat    420
gctgtcattt tgagaggaag ttcttccgca aaattttcaa acagcgcact cattcagtcg    480
attcatcaag cattggaatt gacagatatt ccaacggatg ctgtccaact tatcgaagac    540
acaagcagag agacagccaa agtactgttc catctcaacg aatatataga tgtgttgatc    600
ccaagagggg gaaaaaactt gattgaaaca gtggttagag aatcaactgt ccctgttctt    660
gaaacaggtg caggcaactg tcatgtcttt attgatgaga cagctgatcc gtcaatggcg    720
gaacggatcg ttctaaatgc aaaaacccag cgcccgtctg tgtgcaatgc aatcgagtcc    780
cttttaattc atgaaacatg gtttgaaaga catggggaag ccttgttaca ggccctagtc    840
aatagcgggg ttgagatcat cggtgatcaa gcggtctgtg atgtcttgcc ttccgcgaaa    900
aaggcgacgg aagatgactg gtatacggaa tacttagcat tgacagtcag tgtgaaagtt    960
gtaaaaagtg ttgaggatgc tattagccac attaataaat atgggacgaa gcactcagaa   1020
gcgatcatta cggaaaacag cgagaatgca tcgatttttc ttaacaatgt tgatgctgct   1080
gcagtttatc ataacgcatc gacccgtttt acagacggat ttgaattcgg ctacggtgcg   1140
gaaattggga tcagtacaca aaagcttcac gcaagaggtc cgatgggatt aactgcttta   1200
acatccagca aatattttat ttatgggaat ggtcaaatac gcaaataa                1248

<210> 22
<211> 840
<212> DNA
<213> Bacillus methanolicus

<220>
<223> Bacillus methanolicus MGA3: proI gene (CDS)

<400> 22
atgaagaagc ttacttttgt cggagcaggt tcgatggcgg aagcaatgat ctccggaatg     60
ctaaagagca acctgataga gaaaaacggc atttgggtaa cgaatcgaaa agatgacgac    120
aagctcatcc gcatgaaccg ggagtatggt gtgaatacct cgtacgattt agaaaaattg    180
ttctcgaatg cagatgctgt aattttagcg atgaaaccga aagacgctgc agcagcaatt    240
acccgcatta aagaatactt aacagaagat atgctgatta tttccgttct ggcgggaata    300
tcgatttcca gtattgaaga gctttccggc aaatcgctgg caatcgtaag agcgatgccg    360
aatacatcag cagcaatcag gaaatcagca accgctatag ctgttaataa aaaggttaca    420
gatcaccaaa agaaaatcgt aaaacaaatg tttgaaacaa tcgggctaac aacttttgtt    480
gaagaagaac aacttgatgc tgtaaccggc ctgtccggca gcggcccggc ttatatttat    540
tatcttgtcg aagcaatgga aaaaagtgct gctgaaattg gtcttgaaaa acaagttgca    600
aaacaattaa ttgtgcaaac gctgctcgga gcggcggaaa tgctgacgaa aacatctaaa    660
tcgccggagc agctgcgccg ggaggtaaca agtcccggcg gaacaacaga agccggcatt    720
aaagttctcg aggcccatga cgttcagcat gcttttatat cctgcattaa agaagcaacc    780
gcacaatcta aacgcctagg aagcatgtta aacacagaag cagtaacagt aaagcagtaa    840

<210> 23
<211> 364
<212> PRT
<213> Bacillus methanolicus

<220>
<223> Bacillus methanolicus MGA3: proB protein (data base
      accession AIE60170.1)

<400> 23
Met Asp Lys Lys Arg Ile Val Val Lys Ile Gly Ser Ser Ser Leu Thr
1               5                   10                  15
Asn Ser Lys Gly Glu Ile Asp His Glu Lys Phe Ser Asp His Ile Arg
            20                  25                  30
Ala Ile Ala Ala Leu Arg Ala Glu Gly His Glu Val Leu Ile Val Ser
        35                  40                  45
Ser Gly Ala Val Ala Ala Gly Phe Lys Arg Leu Gly Tyr Pro Ser Arg
    50                  55                  60
Pro Val Thr Ile Lys Gly Lys Gln Ala Ala Ala Ala Val Gly Gln Ser
65                  70                  75                  80
Leu Leu Ile Gln Ala Tyr Met Glu Lys Phe Gly Glu Phe Gly Ile Leu
                85                  90                  95
Pro Ala Gln Ile Leu Leu Thr Arg Asn Asp Phe Ser Lys Lys Glu Arg
            100                 105                 110
Tyr Arg Asn Ala Tyr Ser Thr Ile Met Glu Leu Leu Glu Arg Gly Ile
        115                 120                 125
Val Pro Ile Ile Asn Glu Asn Asp Thr Val Ser Val Glu Glu Leu Thr
    130                 135                 140
Phe Gly Asp Asn Asp Met Leu Ser Ala Leu Val Ser Gly Met Val His
145                 150                 155                 160
Ala Asp Met Leu Ile Ile Leu Thr Asp Ile Asn Gly Leu Tyr Asp Ser
                165                 170                 175
Asn Pro Lys Lys Asn Arg Phe Ala Lys Arg Ile Asn Ile Leu His Glu
            180                 185                 190
Ile Thr Asp Glu Met Met Ala Ala Ala Glu Gly Thr Gly Ser Lys Val
        195                 200                 205
Gly Thr Gly Gly Met Leu Ser Lys Leu Thr Ala Ala Lys Thr Ala Leu
    210                 215                 220
Ser Leu Gly Val Arg Val Phe Ile Gly Lys Gly Lys Gly Ser Arg Lys
225                 230                 235                 240
Leu Leu Asp Ile Leu Glu Gly Arg Gly Asp Gly Thr Tyr Val Gly Lys
                245                 250                 255
Asn Val Ile Pro Ile Lys Asn Ser Lys Gln Trp Ile Ala Phe His Ser
            260                 265                 270
Asp Val Ser Gly Lys Ile Tyr Val Asp Lys Gly Ala Glu Glu Ala Leu
        275                 280                 285
Leu Ser His Gly Lys Ser Leu Leu Pro Ala Gly Ile Tyr Lys Ile Thr
    290                 295                 300
Gly Thr Phe Lys Arg Gly Asp Val Val Glu Val Phe Gly Thr Asn Gly
305                 310                 315                 320
Leu Leu Gly Lys Gly Glu Val Leu Tyr Ser Ser Glu Glu Leu Lys Lys
                325                 330                 335
Val Met Gly Lys Arg Ser Asp Glu Leu Lys Lys Asp Thr Val Met Ala
            340                 345                 350
Ile Glu Val Ile His Arg Asp Lys Trp Val Lys Ala
        355                 360

<210> 24
<211> 415
<212> PRT
<213> Bacillus methanolicus

<220>
<223> Bacillus methanolicus MGA3: proA protein (data base
      accession AIE60169.1)

<400> 24
Met Ser Glu Val Lys Arg Lys Gly Lys Ala Ala Lys Gln Val Ser Tyr
1               5                   10                  15
Ser Leu Val Asn Leu Thr Thr Glu Ala Lys Asn Glu Ala Leu Glu Lys
            20                  25                  30
Ile Ala Val Gln Leu Leu Lys Asp Lys Asn Phe Leu Leu Gln Glu Asn
        35                  40                  45
Gln Lys Asp Ile Glu His Gly Arg Arg Thr Gly Leu Ser Asp Ala Ile
    50                  55                  60
Leu Asp Arg Ile Leu Leu Thr Glu Lys Arg Ile Glu Asp Met Ala Gly
65                  70                  75                  80
Ala Ile Arg Leu Leu Ile Gly Leu Lys Asp Pro Ile Gly Glu Thr Leu
                85                  90                  95
Glu Thr Ile Glu Lys Glu Asn Gly Leu Leu Ile Gln Lys Lys Arg Val
            100                 105                 110
Pro Ile Gly Val Ile Gly Met Ile Tyr Glu Ala Arg Pro Asn Val Thr
        115                 120                 125
Val Asp Ala Ala Thr Leu Ser Leu Lys Thr Gly Asn Ala Val Ile Leu
    130                 135                 140
Arg Gly Ser Ser Ser Ala Lys Phe Ser Asn Ser Ala Leu Ile Gln Ser
145                 150                 155                 160
Ile His Gln Ala Leu Glu Leu Thr Asp Ile Pro Thr Asp Ala Val Gln
                165                 170                 175
Leu Ile Glu Asp Thr Ser Arg Glu Thr Ala Lys Val Leu Phe His Leu
            180                 185                 190
Asn Glu Tyr Ile Asp Val Leu Ile Pro Arg Gly Gly Lys Asn Leu Ile
        195                 200                 205
Glu Thr Val Val Arg Glu Ser Thr Val Pro Val Leu Glu Thr Gly Ala
    210                 215                 220
Gly Asn Cys His Val Phe Ile Asp Glu Thr Ala Asp Pro Ser Met Ala
225                 230                 235                 240
Glu Arg Ile Val Leu Asn Ala Lys Thr Gln Arg Pro Ser Val Cys Asn
                245                 250                 255
Ala Ile Glu Ser Leu Leu Ile His Glu Thr Trp Phe Glu Arg His Gly
            260                 265                 270
Glu Ala Leu Leu Gln Ala Leu Val Asn Ser Gly Val Glu Ile Ile Gly
        275                 280                 285
Asp Gln Ala Val Cys Asp Val Leu Pro Ser Ala Lys Lys Ala Thr Glu
    290                 295                 300
Asp Asp Trp Tyr Thr Glu Tyr Leu Ala Leu Thr Val Ser Val Lys Val
305                 310                 315                 320
Val Lys Ser Val Glu Asp Ala Ile Ser His Ile Asn Lys Tyr Gly Thr
                325                 330                 335
Lys His Ser Glu Ala Ile Ile Thr Glu Asn Ser Glu Asn Ala Ser Ile
            340                 345                 350
Phe Leu Asn Asn Val Asp Ala Ala Ala Val Tyr His Asn Ala Ser Thr
        355                 360                 365
Arg Phe Thr Asp Gly Phe Glu Phe Gly Tyr Gly Ala Glu Ile Gly Ile
    370                 375                 380
Ser Thr Gln Lys Leu His Ala Arg Gly Pro Met Gly Leu Thr Ala Leu
385                 390                 395                 400
Thr Ser Ser Lys Tyr Phe Ile Tyr Gly Asn Gly Gln Ile Arg Lys
                405                 410                 415

<210> 25
<211> 279
<212> PRT
<213> Bacillus methanolicus

<220>
<223> Bacillus methanolicus MGA3: proI protein (data base
      accession AIE60640.1)

<400> 25
Met Lys Lys Leu Thr Phe Val Gly Ala Gly Ser Met Ala Glu Ala Met
1               5                   10                  15
Ile Ser Gly Met Leu Lys Ser Asn Leu Ile Glu Lys Asn Gly Ile Trp
            20                  25                  30
Val Thr Asn Arg Lys Asp Asp Asp Lys Leu Ile Arg Met Asn Arg Glu
        35                  40                  45
Tyr Gly Val Asn Thr Ser Tyr Asp Leu Glu Lys Leu Phe Ser Asn Ala
    50                  55                  60
Asp Ala Val Ile Leu Ala Met Lys Pro Lys Asp Ala Ala Ala Ala Ile
65                  70                  75                  80
Thr Arg Ile Lys Glu Tyr Leu Thr Glu Asp Met Leu Ile Ile Ser Val
                85                  90                  95
Leu Ala Gly Ile Ser Ile Ser Ser Ile Glu Glu Leu Ser Gly Lys Ser
            100                 105                 110
Leu Ala Ile Val Arg Ala Met Pro Asn Thr Ser Ala Ala Ile Arg Lys
        115                 120                 125
Ser Ala Thr Ala Ile Ala Val Asn Lys Lys Val Thr Asp His Gln Lys
    130                 135                 140
Lys Ile Val Lys Gln Met Phe Glu Thr Ile Gly Leu Thr Thr Phe Val
145                 150                 155                 160
Glu Glu Glu Gln Leu Asp Ala Val Thr Gly Leu Ser Gly Ser Gly Pro
                165                 170                 175
Ala Tyr Ile Tyr Tyr Leu Val Glu Ala Met Glu Lys Ser Ala Ala Glu
            180                 185                 190
Ile Gly Leu Glu Lys Gln Val Ala Lys Gln Leu Ile Val Gln Thr Leu
        195                 200                 205
Leu Gly Ala Ala Glu Met Leu Thr Lys Thr Ser Lys Ser Pro Glu Gln
    210                 215                 220
Leu Arg Arg Glu Val Thr Ser Pro Gly Gly Thr Thr Glu Ala Gly Ile
225                 230                 235                 240
Lys Val Leu Glu Ala His Asp Val Gln His Ala Phe Ile Ser Cys Ile
                245                 250                 255
Lys Glu Ala Thr Ala Gln Ser Lys Arg Leu Gly Ser Met Leu Asn Thr
            260                 265                 270
Glu Ala Val Thr Val Lys Gln
        275

<210> 26
<211> 359
<212> PRT
<213> Artificial sequence

<220>
<223> Hypothetical proB protein

<400> 26
Met Lys Lys Lys Arg Ile Val Val Lys Ile Gly Ser Ser Ser Leu Thr
1               5                   10                  15
Asn Ala Lys Gly Glu Ile Asp Gln Glu Lys Leu Ser Asp His Val Glu
            20                  25                  30
Ala Ile Ala Ala Leu Lys Lys Ala Gly His Glu Val Ile Leu Val Ser
        35                  40                  45
Ser Gly Ala Val Ala Ala Gly Phe Arg Lys Leu Gly Tyr Pro Ser Arg
    50                  55                  60
Pro Val Thr Leu Lys Gly Lys Gln Ala Ala Ala Ala Val Gly Gln Ser
65                  70                  75                  80
Leu Leu Ile Gln Ala Tyr Ile Glu Ala Phe Ser Glu Phe Gly Ile Val
                85                  90                  95
Pro Ala Gln Ile Leu Leu Thr Arg Asn Asp Phe Ser Lys Lys Glu Arg
            100                 105                 110
Tyr Lys Asn Ala Tyr Ala Thr Leu Thr Glu Leu Leu Glu Arg Gly Val
        115                 120                 125
Leu Pro Ile Ile Asn Glu Asn Asp Thr Val Ser Val Glu Glu Leu Thr
    130                 135                 140
Phe Gly Asp Asn Asp Met Leu Ser Ala Leu Val Ser Gly Leu Val His
145                 150                 155                 160
Ala Asp Gln Leu Ile Ile Leu Thr Asp Ile Asn Gly Leu Tyr Asp Ser
                165                 170                 175
Asn Pro Arg Thr Asn Pro Asp Ala Lys Arg Tyr Asp Phe Leu Pro Glu
            180                 185                 190
Ile Thr Asp Glu Leu Leu Ala Ala Ala Gly Gly Ser Gly Ser Lys Val
        195                 200                 205
Gly Thr Gly Gly Met Lys Ser Lys Leu Leu Ala Ala Lys Thr Ala Leu
    210                 215                 220
Ser Leu Gly Val Lys Val Phe Ile Gly Thr Gly Ser Gly Glu Glu Lys
225                 230                 235                 240
Leu Leu Asp Ile Leu Glu Gly Lys Gly Asp Gly Thr Tyr Ile Gly Asn
                245                 250                 255
Pro Phe Leu Ser Leu Asn Asn Lys Lys Gln Trp Ile Ala Leu His Ser
            260                 265                 270
Glu Val Ser Gly Lys Ile Tyr Ile Asp Glu Gly Ala Glu Glu Ala Leu
        275                 280                 285
Leu Tyr Lys Gly Lys Ser Leu Leu Pro Ala Gly Val Thr Lys Val Glu
    290                 295                 300
Gly Thr Phe Glu Lys Gly Asp Val Val Glu Val Tyr Gly Glu Lys Gly
305                 310                 315                 320
Leu Ile Gly Lys Gly Gln Val Asn Tyr Ser Ser Glu Glu Leu Lys Lys
                325                 330                 335
Val Lys Gly Lys Arg Ser Asp Glu Ala Lys Arg Ile Glu Val Ile His
            340                 345                 350
Arg Asp Asn Trp Val Lys Leu
        355
