                         SEQUENCE LISTING

<110>  BIODURO(JIANGSU) CO., LTD.
 
<120>  ANTIBODY AGAINST SARS-COV-2

<130>  C21W1191

<150>  PCT/CN2021/073917
<151>  2021-01-27

<160>  50    

<170>  PatentIn version 3.5

<210>  1
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH60 CDR1

<400>  1

Gly Arg Thr Phe Arg Val Asn Leu Met Gly 
1               5                   10  


<210>  2
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH60 CDR2

<400>  2

Ser Ile Asn Gly Phe Asp Asp Ile Thr Tyr Tyr 
1               5                   10      


<210>  3
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH60 CDR3

<400>  3

Ala Tyr Asp Ser Asp Tyr Asp Gly Arg Leu Phe Asn Tyr Trp Gly 
1               5                   10                  15  


<210>  4
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH35 CDR1

<400>  4

Gly Ser Ile Tyr Ser Phe Asn Phe Met Gly 
1               5                   10  


<210>  5
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH35 CDR2

<400>  5

Thr Ile Asn Ser Phe Asp Asp Ile Thr Tyr Tyr 
1               5                   10      


<210>  6
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH35 CDR3

<400>  6

Val Leu Gly Glu Arg Thr Gly Ile Ser Tyr Gly Ser Ala Phe Asp Tyr 
1               5                   10                  15      


Trp Gly 
        


<210>  7
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH79 CDR1

<400>  7

Gly Phe Thr Ser Arg Asn Tyr Phe Met Gly 
1               5                   10  


<210>  8
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH79 CDR2

<400>  8

Thr Ile Asn Ser Leu Ser Ser Ile Thr Tyr Tyr 
1               5                   10      


<210>  9
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH79 CDR3

<400>  9

Val Tyr Thr Pro Thr Thr Gly Pro Gly Glu Gly Ser Tyr Thr Pro Trp 
1               5                   10                  15      


His Asp Tyr Trp Gly 
            20      


<210>  10
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH80 CDR1

<400>  10

Gly Phe Ile Ser Asn Phe Asn Leu Met Gly 
1               5                   10  


<210>  11
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH80 CDR2

<400>  11

Thr Ile Asn Ser Phe Asp Asp Ile Thr Tyr Tyr 
1               5                   10      


<210>  12
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>   VHH80 CDR3

<400>  12

Ala Glu Val Arg Ser Ser Leu Asp Tyr Ala Leu Trp Thr Ser Arg Arg 
1               5                   10                  15      


Ser Ala Phe Ser Tyr Trp Gly 
            20              


<210>  13
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH34 CDR1

<400>  13

Gly Phe Ile Tyr Ser Phe Asn Ile Met Gly 
1               5                   10  


<210>  14
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH34 CDR2

<400>  14

Ser Ile Asn Trp Phe Ser Asp Ile Thr Tyr Tyr 
1               5                   10      


<210>  15
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH34 CDR3

<400>  15

Ala Tyr Leu Leu Arg Gly Asp Asp Arg Tyr Tyr Ala Thr Tyr Ser Tyr 
1               5                   10                  15      


Trp Gly 
        


<210>  16
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH43 CDR1

<400>  16

Gly Phe Ile Ser Asp Ala Asp Ile Met Gly 
1               5                   10  


<210>  17
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH43 CDR2

<400>  17

Ser Ile Asn Ser Tyr Asp Ser Ile Thr Tyr Tyr 
1               5                   10      


<210>  18
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH43 CDR3

<400>  18

Val Arg Val His Ser Arg Asp Phe Ser Tyr Trp Gly 
1               5                   10          


<210>  19
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH82 CDR1

<400>  19

Gly Phe Ile Tyr Ser Phe Asn Ile Met Gly 
1               5                   10  


<210>  20
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH82 CDR2

<400>  20

Ser Ile Ser Ser Tyr Asp Asp Ile Thr Tyr Tyr 
1               5                   10      


<210>  21
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH82 CDR3

<400>  21

Ala Tyr Leu Leu Arg Gly Asp Asp Arg Tyr Tyr Ala Thr Tyr Ser Tyr 
1               5                   10                  15      


Trp Gly 
        


<210>  22
<211>  121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of VHH60

<400>  22

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Arg Val Asn 
            20                  25                  30          


Leu Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Gly Arg Glu Leu Val 
        35                  40                  45              


Ala Ser Ile Asn Gly Phe Asp Asp Ile Thr Tyr Tyr Pro Asp Ser Val 
    50                  55                  60                  


Glu Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Arg Met Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Tyr Asp Ser Asp Tyr Asp Gly Arg Leu Phe Asn Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120     


<210>  23
<211>  124
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of VHH35

<400>  23

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Tyr Ser Phe Asn 
            20                  25                  30          


Phe Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Gly Arg Glu Leu Val 
        35                  40                  45              


Ala Thr Ile Asn Ser Phe Asp Asp Ile Thr Tyr Tyr Pro Asp Ser Val 
    50                  55                  60                  


Glu Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Arg Met Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Val Leu Gly Glu Arg Thr Gly Ile Ser Tyr Gly Ser Ala Phe Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 


<210>  24
<211>  127
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of VHH79

<400>  24

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Ser Arg Asn Tyr 
            20                  25                  30          


Phe Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Gly Arg Glu Leu Val 
        35                  40                  45              


Ala Thr Ile Asn Ser Leu Ser Ser Ile Thr Tyr Tyr Pro Asp Ser Val 
    50                  55                  60                  


Glu Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Arg Met Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Val Tyr Thr Pro Thr Thr Gly Pro Gly Glu Gly Ser Tyr Thr Pro 
            100                 105                 110         


Trp His Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125         


<210>  25
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of VHH80

<400>  25

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ile Ser Asn Phe Asn 
            20                  25                  30          


Leu Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Gly Arg Glu Leu Val 
        35                  40                  45              


Ala Thr Ile Asn Ser Phe Asp Asp Ile Thr Tyr Tyr Pro Asp Ser Val 
    50                  55                  60                  


Glu Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Arg Met Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Glu Val Arg Ser Ser Leu Asp Tyr Ala Leu Trp Thr Ser Arg 
            100                 105                 110         


Arg Ser Ala Phe Ser Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser 
        115                 120                 125             


Ser 
    


<210>  26
<211>  124
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of VHH34

<400>  26

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ile Tyr Ser Phe Asn 
            20                  25                  30          


Ile Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Gly Arg Glu Leu Val 
        35                  40                  45              


Ala Ser Ile Asn Trp Phe Ser Asp Ile Thr Tyr Tyr Pro Asp Ser Val 
    50                  55                  60                  


Glu Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Arg Met Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Tyr Leu Leu Arg Gly Asp Asp Arg Tyr Tyr Ala Thr Tyr Ser 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 


<210>  27
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of VHH43

<400>  27

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ile Ser Asp Ala Asp 
            20                  25                  30          


Ile Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Gly Arg Glu Leu Val 
        35                  40                  45              


Ala Ser Ile Asn Ser Tyr Asp Ser Ile Thr Tyr Tyr Pro Asp Ser Val 
    50                  55                  60                  


Glu Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Arg Met Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Val Arg Val His Ser Arg Asp Phe Ser Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Gln Val Thr Val Ser Ser 
        115             


<210>  28
<211>  124
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of VHH82

<400>  28

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ile Tyr Ser Phe Asn 
            20                  25                  30          


Ile Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Gly Arg Glu Leu Val 
        35                  40                  45              


Ala Ser Ile Ser Ser Tyr Asp Asp Ile Thr Tyr Tyr Pro Asp Ser Val 
    50                  55                  60                  


Glu Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Arg Met Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Tyr Leu Leu Arg Gly Asp Asp Arg Tyr Tyr Ala Thr Tyr Ser 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 


<210>  29
<211>  363
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding VHH60

<400>  29
gaggtgcagc tggtggaaag cggcggagga ctggtgcaac ccggcggctc tctgagactg       60

agctgtgccg cctccggcag aacctttcgt gttaatctta tgggctggtt cagacaagcc      120

cccggcaagg gcagagagct ggtggctagt attaacgggt ttgatgatat tacctattac      180

cccgactccg tggagggaag attcaccatc tctagagaca acgccaagag gatggtgtac      240

ctccagatga actctctgag agccgaggac acagccgtgt attactgcgc cgcttacgac      300

tctgactacg acggtcgtct gtttaattat tggggacaag gcacccaagt gaccgtgagc      360

tcc                                                                    363


<210>  30
<211>  372
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding VHH35

<400>  30
gaggtgcagc tggtggaaag cggcggagga ctggtgcaac ccggcggctc tctgagactg       60

agctgtgccg cctccggcag tatctatagt tttaatttta tgggctggtt cagacaagcc      120

cccggcaagg gcagagagct ggtggctact attaactcgt ttgatgatat tacctattac      180

cccgactccg tggagggaag attcaccatc tctagagaca acgccaagag gatggtgtac      240

ctccagatga actctctgag agccgaggac acagccgtgt attactgcgc cgttctgggt      300

gaacgtactg gtatctctta cggttctgct tttgattatt ggggacaagg cacccaagtg      360

accgtgagct cc                                                          372


<210>  31
<211>  381
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding VHH79

<400>  31
gaggtgcagc tggtggaaag cggcggagga ctggtgcaac ccggcggctc tctgagactg       60

agctgtgccg cctccggctt tacctctcgt aattatttta tgggctggtt cagacaagcc      120

cccggcaagg gcagagagct ggtggctact attaactcgc ttagcagcat tacctattac      180

cccgactccg tggagggaag attcaccatc tctagagaca acgccaagag gatggtgtac      240

ctccagatga actctctgag agccgaggac acagccgtgt attactgcgc cgtttacact      300

ccgactactg gtccgggtga aggttcttac actccgtggc atgactattg gggacaaggc      360

acccaagtga ccgtgagctc c                                                381


<210>  32
<211>  387
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding VHH80

<400>  32
gaggtgcagc tggtggaaag cggcggagga ctggtgcaac ccggcggctc tctgagactg       60

agctgtgccg cctccggctt tatctctaac tttaatctta tgggctggtt cagacaagcc      120

cccggcaagg gcagagagct ggtggctact attaactcgt ttgatgatat tacctattac      180

cccgactccg tggagggaag attcaccatc tctagagaca acgccaagag gatggtgtac      240

ctccagatga actctctgag agccgaggac acagccgtgt attactgcgc cgctgaagtt      300

cgttcttctc tggactacgc tctgtggact tctcgtcgtt ctgcttttag ttattgggga      360

caaggcaccc aagtgaccgt gagctcc                                          387


<210>  33
<211>  372
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding VHH34

<400>  33
gaggtgcagc tggtggaaag cggcggagga ctggtgcaac ccggcggctc tctgagactg       60

agctgtgccg cctccggctt tatctatagt tttaatatta tgggctggtt cagacaagcc      120

cccggcaagg gcagagagct ggtggctagt attaactggt ttagcgatat tacctattac      180

cccgactccg tggagggaag attcaccatc tctagagaca acgccaagag gatggtgtac      240

ctccagatga actctctgag agccgaggac acagccgtgt attactgcgc cgcttacctg      300

ctgcgtggtg acgaccgtta ctacgctact tatagctatt ggggacaagg cacccaagtg      360

accgtgagct cc                                                          372


<210>  34
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding VHH43

<400>  34
gaggtgcagc tggtggaaag cggcggagga ctggtgcaac ccggcggctc tctgagactg       60

agctgtgccg cctccggctt tatctctgac gctgatatta tgggctggtt cagacaagcc      120

cccggcaagg gcagagagct ggtggctagt attaactcgt atgatagcat tacctattac      180

cccgactccg tggagggaag attcaccatc tctagagaca acgccaagag gatggtgtac      240

ctccagatga actctctgag agccgaggac acagccgtgt attactgcgc cgttcgtgtt      300

cattctcgtg actttagcta ttggggacaa ggcacccaag tgaccgtgag ctcc            354


<210>  35
<211>  372
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding VHH82

<400>  35
gaggtgcagc tggtggaaag cggcggagga ctggtgcaac ccggcggctc tctgagactg       60

agctgtgccg cctccggctt tatctatagt tttaatatta tgggctggtt cagacaagcc      120

cccggcaagg gcagagagct ggtggctagt attagctcgt atgatgatat tacctattac      180

cccgactccg tggagggaag attcaccatc tctagagaca acgccaagag gatggtgtac      240

ctccagatga actctctgag agccgaggac acagccgtgt attactgcgc cgcttacctg      300

ctgcgtggtg acgaccgtta ctacgctact tatagctatt ggggacaagg cacccaagtg      360

accgtgagct cc                                                          372


<210>  36
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  36

Gly Ser Ser Ser Ser Gly Ser Ser Ser Ser Gly Ser Ser Ser Ser 
1               5                   10                  15  


<210>  37
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Signal peptide

<400>  37

Met Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala Leu Phe Arg Gly 
1               5                   10                  15      


Val Gln Cys 
            


<210>  38
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  38

Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10          


<210>  39
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SARS2-N-F

<400>  39
taatcagaca aggaactgat ta                                                22


<210>  40
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SARS2-N-R

<400>  40
cgaaggtgtg acttccatg                                                    19


<210>  41
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SARS2-N-P

<400>  41
gcaaattgtg caatttgcgg                                                   20


<210>  42
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hGAPDH-F

<400>  42
cagcctcaag atcatcagca                                                   20


<210>  43
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hGAPDH-R

<400>  43
tgtggtcatg agtccttcca                                                   20


<210>  44
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hGAPDH-P

<400>  44
ctgcttagca cccctggcca                                                   20


<210>  45
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hACE2-F

<400>  45
cattggagca agtgttggat ctt                                               23


<210>  46
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hACE2-R

<400>  46
gagctaatgc atgccattct ca                                                22


<210>  47
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hACE2-P

<400>  47
cttgcagcta caccagttcc caggca                                            26


<210>  48
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mGAPDH-F

<400>  48
tgcaccacca actgcttag                                                    19


<210>  49
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mGAPDH-R

<400>  49
ggatgcaggg atgatgttc                                                    19


<210>  50
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mGAPDH-P

<400>  50
cagaagactg tggatggccc ctc                                               23


