                         SEQUENCE LISTING

<110>  CELLDEX THERAPEUTICS, INC.
 
<120>  ANTI-KIT ANTIBODIES AND USES THEREOF

<130>  12638-170-228

<140>  TBA
<141>  

<150>  US 63/140,642 
<151>  2021-01-22

<150>  US 63/238,649
<151>  2021-08-30

<160>  40    

<170>  PatentIn version 3.5

<210>  1
<211>  972
<212>  PRT
<213>  Homo sapiens

<220>
<223>  human KIT receptor 

<400>  1

Met Arg Gly Ala Arg Gly Ala Trp Asp Phe Leu Cys Val Leu Leu Leu 
1               5                   10                  15      


Leu Leu Arg Val Gln Thr Gly Ser Ser Gln Pro Ser Val Ser Pro Gly 
            20                  25                  30          


Glu Pro Ser Pro Pro Ser Ile His Pro Gly Lys Ser Asp Leu Ile Val 
        35                  40                  45              


Arg Val Gly Asp Glu Ile Arg Leu Leu Cys Thr Asp Pro Gly Phe Val 
    50                  55                  60                  


Lys Trp Thr Phe Glu Ile Leu Asp Glu Thr Asn Glu Asn Lys Gln Asn 
65                  70                  75                  80  


Glu Trp Ile Thr Glu Lys Ala Glu Ala Thr Asn Thr Gly Lys Tyr Thr 
                85                  90                  95      


Cys Thr Asn Lys His Gly Leu Ser Asn Ser Ile Tyr Val Phe Val Arg 
            100                 105                 110         


Asp Pro Ala Lys Leu Phe Leu Val Asp Arg Ser Leu Tyr Gly Lys Glu 
        115                 120                 125             


Asp Asn Asp Thr Leu Val Arg Cys Pro Leu Thr Asp Pro Glu Val Thr 
    130                 135                 140                 


Asn Tyr Ser Leu Lys Gly Cys Gln Gly Lys Pro Leu Pro Lys Asp Leu 
145                 150                 155                 160 


Arg Phe Ile Pro Asp Pro Lys Ala Gly Ile Met Ile Lys Ser Val Lys 
                165                 170                 175     


Arg Ala Tyr His Arg Leu Cys Leu His Cys Ser Val Asp Gln Glu Gly 
            180                 185                 190         


Lys Ser Val Leu Ser Glu Lys Phe Ile Leu Lys Val Arg Pro Ala Phe 
        195                 200                 205             


Lys Ala Val Pro Val Val Ser Val Ser Lys Ala Ser Tyr Leu Leu Arg 
    210                 215                 220                 


Glu Gly Glu Glu Phe Thr Val Thr Cys Thr Ile Lys Asp Val Ser Ser 
225                 230                 235                 240 


Ser Val Tyr Ser Thr Trp Lys Arg Glu Asn Ser Gln Thr Lys Leu Gln 
                245                 250                 255     


Glu Lys Tyr Asn Ser Trp His His Gly Asp Phe Asn Tyr Glu Arg Gln 
            260                 265                 270         


Ala Thr Leu Thr Ile Ser Ser Ala Arg Val Asn Asp Ser Gly Val Phe 
        275                 280                 285             


Met Cys Tyr Ala Asn Asn Thr Phe Gly Ser Ala Asn Val Thr Thr Thr 
    290                 295                 300                 


Leu Glu Val Val Asp Lys Gly Phe Ile Asn Ile Phe Pro Met Ile Asn 
305                 310                 315                 320 


Thr Thr Val Phe Val Asn Asp Gly Glu Asn Val Asp Leu Ile Val Glu 
                325                 330                 335     


Tyr Glu Ala Phe Pro Lys Pro Glu His Gln Gln Trp Ile Tyr Met Asn 
            340                 345                 350         


Arg Thr Phe Thr Asp Lys Trp Glu Asp Tyr Pro Lys Ser Glu Asn Glu 
        355                 360                 365             


Ser Asn Ile Arg Tyr Val Ser Glu Leu His Leu Thr Arg Leu Lys Gly 
    370                 375                 380                 


Thr Glu Gly Gly Thr Tyr Thr Phe Leu Val Ser Asn Ser Asp Val Asn 
385                 390                 395                 400 


Ala Ala Ile Ala Phe Asn Val Tyr Val Asn Thr Lys Pro Glu Ile Leu 
                405                 410                 415     


Thr Tyr Asp Arg Leu Val Asn Gly Met Leu Gln Cys Val Ala Ala Gly 
            420                 425                 430         


Phe Pro Glu Pro Thr Ile Asp Trp Tyr Phe Cys Pro Gly Thr Glu Gln 
        435                 440                 445             


Arg Cys Ser Ala Ser Val Leu Pro Val Asp Val Gln Thr Leu Asn Ser 
    450                 455                 460                 


Ser Gly Pro Pro Phe Gly Lys Leu Val Val Gln Ser Ser Ile Asp Ser 
465                 470                 475                 480 


Ser Ala Phe Lys His Asn Gly Thr Val Glu Cys Lys Ala Tyr Asn Asp 
                485                 490                 495     


Val Gly Lys Thr Ser Ala Tyr Phe Asn Phe Ala Phe Lys Glu Gln Ile 
            500                 505                 510         


His Pro His Thr Leu Phe Thr Pro Leu Leu Ile Gly Phe Val Ile Val 
        515                 520                 525             


Ala Gly Met Met Cys Ile Ile Val Met Ile Leu Thr Tyr Lys Tyr Leu 
    530                 535                 540                 


Gln Lys Pro Met Tyr Glu Val Gln Trp Lys Val Val Glu Glu Ile Asn 
545                 550                 555                 560 


Gly Asn Asn Tyr Val Tyr Ile Asp Pro Thr Gln Leu Pro Tyr Asp His 
                565                 570                 575     


Lys Trp Glu Phe Pro Arg Asn Arg Leu Ser Phe Gly Lys Thr Leu Gly 
            580                 585                 590         


Ala Gly Ala Phe Gly Lys Val Val Glu Ala Thr Ala Tyr Gly Leu Ile 
        595                 600                 605             


Lys Ser Asp Ala Ala Met Thr Val Ala Val Lys Met Leu Lys Pro Ser 
    610                 615                 620                 


Ala His Leu Thr Glu Arg Glu Ala Leu Met Ser Glu Leu Lys Val Leu 
625                 630                 635                 640 


Ser Tyr Leu Gly Asn His Met Asn Ile Val Asn Leu Leu Gly Ala Cys 
                645                 650                 655     


Thr Ile Gly Gly Pro Thr Leu Val Ile Thr Glu Tyr Cys Cys Tyr Gly 
            660                 665                 670         


Asp Leu Leu Asn Phe Leu Arg Arg Lys Arg Asp Ser Phe Ile Cys Ser 
        675                 680                 685             


Lys Gln Glu Asp His Ala Glu Ala Ala Leu Tyr Lys Asn Leu Leu His 
    690                 695                 700                 


Ser Lys Glu Ser Ser Cys Ser Asp Ser Thr Asn Glu Tyr Met Asp Met 
705                 710                 715                 720 


Lys Pro Gly Val Ser Tyr Val Val Pro Thr Lys Ala Asp Lys Arg Arg 
                725                 730                 735     


Ser Val Arg Ile Gly Ser Tyr Ile Glu Arg Asp Val Thr Pro Ala Ile 
            740                 745                 750         


Met Glu Asp Asp Glu Leu Ala Leu Asp Leu Glu Asp Leu Leu Ser Phe 
        755                 760                 765             


Ser Tyr Gln Val Ala Lys Gly Met Ala Phe Leu Ala Ser Lys Asn Cys 
    770                 775                 780                 


Ile His Arg Asp Leu Ala Ala Arg Asn Ile Leu Leu Thr His Gly Arg 
785                 790                 795                 800 


Ile Thr Lys Ile Cys Asp Phe Gly Leu Ala Arg Asp Ile Lys Asn Asp 
                805                 810                 815     


Ser Asn Tyr Val Val Lys Gly Asn Ala Arg Leu Pro Val Lys Trp Met 
            820                 825                 830         


Ala Pro Glu Ser Ile Phe Asn Cys Val Tyr Thr Phe Glu Ser Asp Val 
        835                 840                 845             


Trp Ser Tyr Gly Ile Phe Leu Trp Glu Leu Phe Ser Leu Gly Ser Ser 
    850                 855                 860                 


Pro Tyr Pro Gly Met Pro Val Asp Ser Lys Phe Tyr Lys Met Ile Lys 
865                 870                 875                 880 


Glu Gly Phe Arg Met Leu Ser Pro Glu His Ala Pro Ala Glu Met Tyr 
                885                 890                 895     


Asp Ile Met Lys Thr Cys Trp Asp Ala Asp Pro Leu Lys Arg Pro Thr 
            900                 905                 910         


Phe Lys Gln Ile Val Gln Leu Ile Glu Lys Gln Ile Ser Glu Ser Thr 
        915                 920                 925             


Asn His Ile Tyr Ser Asn Leu Ala Asn Cys Ser Pro Asn Arg Gln Lys 
    930                 935                 940                 


Pro Val Val Asp His Ser Val Arg Ile Asn Ser Val Gly Ser Thr Ala 
945                 950                 955                 960 


Ser Ser Ser Gln Pro Leu Leu Val His Asp Asp Val 
                965                 970         


<210>  2
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain CDR1 of anti-KIT antibody (VL-CDR1 - AbM)

<400>  2

Lys Ala Ser Gln Asn Val Arg Thr Asn Val Ala 
1               5                   10      


<210>  3
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain CDR2 of anti-KIT antibody (VL-CDR2 - AbM)

<400>  3

Ser Ala Ser Tyr Arg Tyr Ser 
1               5           


<210>  4
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain CDR3 of anti-KIT antibody (VL-CDR3 - AbM)

<400>  4

Gln Gln Tyr Asn Ser Tyr Pro Arg Thr 
1               5                   


<210>  5
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain CDR1 of anti-KIT antibody (VH-CDR1)

<400>  5

Asp Tyr Tyr Ile Asn 
1               5   


<210>  6
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain CDR2 of anti-KIT antibody (VH-CDR2)

<400>  6

Arg Ile Tyr Pro Gly Ser Gly Asn Thr Tyr Tyr Asn Glu Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  7
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain CDR3 of anti-KIT antibody (VH-CDR3)

<400>  7

Gly Val Tyr Tyr Phe Asp Tyr 
1               5           


<210>  8
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain variable region of anti-KIT antibody (VH1)

<400>  8

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Leu Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Tyr Ile Asn Trp Val Lys Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Ala Arg Ile Tyr Pro Gly Ser Gly Asn Thr Tyr Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Arg Ala Thr Leu Thr Ala Glu Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Arg Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Gly Val Tyr Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Thr Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  9
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain variable region of anti-KIT antibody (VH2)

<400>  9

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Tyr Ile Asn Trp Val Lys Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Ala Arg Ile Tyr Pro Gly Ser Gly Asn Thr Tyr Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Arg Ala Thr Leu Thr Ala Glu Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Gly Val Tyr Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Thr Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  10
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain variable region of anti-KIT antibody (VH3)

<400>  10

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Tyr Ile Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Ala Arg Ile Tyr Pro Gly Ser Gly Asn Thr Tyr Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Arg Ala Thr Leu Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Gly Val Tyr Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Thr Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  11
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain variable region of anti-KIT antibody (VH4)

<400>  11

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Tyr Ile Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Ala Arg Ile Tyr Pro Gly Ser Gly Asn Thr Tyr Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Arg Ala Thr Ile Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Gly Val Tyr Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Thr Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  12
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain variable region of anti-KIT antibody (VH5)

<400>  12

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Tyr Ile Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Ala Arg Ile Tyr Pro Gly Ser Gly Asn Thr Tyr Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Gly Val Tyr Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Thr Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  13
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain variable region of anti-KIT antibody (VL1)

<400>  13

Asp Ile Val Met Thr Gln Ser Pro Ser Phe Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asn Val Arg Thr Asn 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Ala Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Tyr Arg Tyr Ser Gly Val Pro Asp Arg Phe Thr Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ser 
65                  70                  75                  80  


Glu Asp Phe Ala Asp Tyr Phe Cys Gln Gln Tyr Asn Ser Tyr Pro Arg 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  14
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain variable region of anti-KIT antibody (VL2)

<400>  14

Asp Ile Val Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asn Val Arg Thr Asn 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Ala Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Tyr Arg Tyr Ser Gly Val Pro Asp Arg Phe Thr Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Asp Tyr Phe Cys Gln Gln Tyr Asn Ser Tyr Pro Arg 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  15
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain variable region of anti-KIT antibody (VL3)

<400>  15

Asp Ile Val Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asn Val Arg Thr Asn 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Ala Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Tyr Arg Tyr Ser Gly Val Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Asp Tyr Phe Cys Gln Gln Tyr Asn Ser Tyr Pro Arg 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  16
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain variable region of anti-KIT antibody (VL4)

<400>  16

Asp Ile Val Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asn Val Arg Thr Asn 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Ser Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Tyr Arg Tyr Ser Gly Val Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Asn Ser Tyr Pro Arg 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  17
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain variable region consensus sequence

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  Xaa can be any amino acid; or in specific enbodiment, it can be
 amino acid with an aromatic or aliphatic hydroxyl side chain; or in 
another specific embodiment, Xaa is the amino acid Phe or Ser

<220>
<221>  misc_feature
<222>  (46)..(46)
<223>  Xaa can be any amino acid; or in specific enbodiment, it can be
 amino acid with an aliphatic or aliphatic hydroxyl side chain; or in 
another specific embodiment, Xaa is the amino acid Ala or Ser

<220>
<221>  misc_feature
<222>  (63)..(63)
<223>  Xaa can be any amino acid; or in specific enbodiment, it can be
 amino acid with an aliphatic or aliphatic hydroxyl side chain; or in 
another specific embodiment, Xaa is the amino acid Thr or Ser

<220>
<221>  misc_feature
<222>  (80)..(80)
<223>  Xaa can be any amino acid; or in specific enbodiment, it can be
 amino acid with an aliphatic or aliphatic hydroxyl side chain; or in 
another specific embodiment, Xaa is the amino acid Ser or Pro

<220>
<221>  misc_feature
<222>  (85)..(85)
<223>  Xaa can be any amino acid; or in specific enbodiment, it can be
 amino acid with a charged or acidic side chain; or in another specific
 embodiment, Xaa is the amino acid Asp or Thr

<220>
<221>  misc_feature
<222>  (87)..(87)
<223>  Xaa can be any amino acid; or in specific enbodiment, it can be
 amino acid with an aromatic side chain; or in another specific 
embodiment, Xaa is the amino acid Phe or Tyr

<400>  17

Asp Ile Val Met Thr Gln Ser Pro Ser Xaa Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asn Val Arg Thr Asn 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Xaa Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Tyr Arg Tyr Ser Gly Val Pro Asp Arg Phe Xaa Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Xaa 
65                  70                  75                  80  


Glu Asp Phe Ala Xaa Tyr Xaa Cys Gln Gln Tyr Asn Ser Tyr Pro Arg 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  18
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain variable region consensus sequence

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Xaa can be any amino acid; or in specific enbodiment, it can be
 amino acid with an aliphatic side chain; or in another specific 
embodiment, Xaa is the amino acid Leu or Val

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  Xaa can be any amino acid; or in specific enbodiment, it can be
 amino acid with an aliphatic side chain; or in another specific 
embodiment, Xaa is the amino acid Leu or Val

<220>
<221>  misc_feature
<222>  (38)..(38)
<223>  Xaa can be any amino acid; or in specific enbodiment, it can be
 amino acid with a polar or basic side chain; or in another specific 
embodiment, Xaa is the amino acid Lys or Arg

<220>
<221>  misc_feature
<222>  (68)..(68)
<223>  Xaa can be any amino acid; or in specific enbodiment, it can be
 amino acid with an aliphatic side chain; or in another specific 
embodiment, Xaa is the amino acid Val or Ala

<220>
<221>  misc_feature
<222>  (70)..(70)
<223>  Xaa can be any amino acid; or in specific enbodiment, it can be
 amino acid with an aliphatic side chain; or in another specific 
embodiment, Xaa is the amino acid Leu or Ile
<220>
<221>  misc_feature
<222>  (73)..(73)
<223>  Xaa can be any amino acid; or in specific enbodiment, it can be
 amino acid with an acidic side chain; or in another specific 
embodiment, Xaa is the amino acid Glu or Asp

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  Xaa can be any amino acid; or in specific enbodiment, it can be
 amino acid with an acidic or amide derivative side chain; or in another
 specific embodiment, Xaa is the amino acid Gln or Glu

<220>
<221>  misc_feature
<222>  (91)..(91)
<223>  Xaa can be any amino acid; or in specific enbodiment, it can be
 amino acid with an aliphatic hydroxyl side chain; or in another
 specific embodiment, Xaa is the amino acid Ser or Thr

<400>  18

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Xaa Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Xaa Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Tyr Ile Asn Trp Val Xaa Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Ala Arg Ile Tyr Pro Gly Ser Gly Asn Thr Tyr Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Arg Xaa Thr Xaa Thr Ala Xaa Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Xaa Leu Ser Ser Leu Arg Ser Glu Asp Xaa Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Gly Val Tyr Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Thr Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  19
<211>  464
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain sequence with mutations

<400>  19

Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      


Val His Ser Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp Tyr Tyr Ile Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    50                  55                  60                  


Glu Trp Ile Ala Arg Ile Tyr Pro Gly Ser Gly Asn Thr Tyr Tyr Asn 
65                  70                  75                  80  


Glu Lys Phe Lys Gly Arg Ala Thr Leu Thr Ala Asp Lys Ser Thr Ser 
                85                  90                  95      


Thr Ala Tyr Met Gln Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Phe Cys Ala Arg Gly Val Tyr Tyr Phe Asp Tyr Trp Gly Gln Gly 
        115                 120                 125             


Thr Thr Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe 
    130                 135                 140                 


Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu 
145                 150                 155                 160 


Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp 
                165                 170                 175     


Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu 
            180                 185                 190         


Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser 
        195                 200                 205             


Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro 
    210                 215                 220                 


Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys 
225                 230                 235                 240 


Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Ala Gln Gly Gly Pro 
                245                 250                 255     


Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Tyr Ile Thr 
            260                 265                 270         


Arg Glu Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 
        275                 280                 285             


Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn 
    290                 295                 300                 


Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val 
305                 310                 315                 320 


Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu 
                325                 330                 335     


Tyr Lys Cys Gln Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys 
            340                 345                 350         


Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
        355                 360                 365             


Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr 
    370                 375                 380                 


Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
385                 390                 395                 400 


Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
                405                 410                 415     


Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys 
            420                 425                 430         


Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
        435                 440                 445             


Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
    450                 455                 460                 


<210>  20
<211>  234
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain sequence

<400>  20

Met Ser Val Pro Thr Gln Val Leu Gly Leu Leu Leu Leu Trp Leu Thr 
1               5                   10                  15      


Asp Ala Arg Cys Asp Ile Val Met Thr Gln Ser Pro Ser Ser Leu Ser 
            20                  25                  30          


Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asn 
        35                  40                  45              


Val Arg Thr Asn Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
    50                  55                  60                  


Lys Ala Leu Ile Tyr Ser Ala Ser Tyr Arg Tyr Ser Gly Val Pro Asp 
65                  70                  75                  80  


Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
                85                  90                  95      


Ser Leu Gln Pro Glu Asp Phe Ala Asp Tyr Phe Cys Gln Gln Tyr Asn 
            100                 105                 110         


Ser Tyr Pro Arg Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg 
        115                 120                 125             


Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln 
    130                 135                 140                 


Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
145                 150                 155                 160 


Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser 
                165                 170                 175     


Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr 
            180                 185                 190         


Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys 
        195                 200                 205             


His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro 
    210                 215                 220                 


Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230                 


<210>  21
<211>  445
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb1 full-length heavy chain sequence (including Fc)

<400>  21

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Tyr Ile Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Ala Arg Ile Tyr Pro Gly Ser Gly Asn Thr Tyr Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Arg Ala Thr Leu Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Gly Val Tyr Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Thr Val 
            100                 105                 110         


Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala 
        115                 120                 125             


Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu 
    130                 135                 140                 


Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly 
145                 150                 155                 160 


Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser 
                165                 170                 175     


Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu 
            180                 185                 190         


Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr 
        195                 200                 205             


Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr 
    210                 215                 220                 


Cys Pro Pro Cys Pro Ala Pro Glu Ala Gln Gly Gly Pro Ser Val Phe 
225                 230                 235                 240 


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Tyr Ile Thr Arg Glu Pro 
                245                 250                 255     


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
            260                 265                 270         


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
        275                 280                 285             


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
    290                 295                 300                 


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
305                 310                 315                 320 


Gln Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
                325                 330                 335     


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
            340                 345                 350         


Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
        355                 360                 365             


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
    370                 375                 380                 


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
385                 390                 395                 400 


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
                405                 410                 415     


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
            420                 425                 430         


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
        435                 440                 445 


<210>  22
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb1 full-length light chain sequence

<400>  22

Asp Ile Val Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asn Val Arg Thr Asn 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Ala Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Tyr Arg Tyr Ser Gly Val Pro Asp Arg Phe Thr Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Asp Tyr Phe Cys Gln Gln Tyr Asn Ser Tyr Pro Arg 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  23
<211>  1392
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA heavy chain sequence of an anti-KIT antibody

<400>  23
atggagtggt cctgggtgtt cctgttcttt ctgtccgtga ccacaggcgt gcacagccag       60

gtgcagctgg tgcagtctgg agctgaggtg aagaagccag gagcttctgt gaagctgtcc      120

tgcaaggcca gcggctacac cttcacagac tactatatca actgggtgag acaggctcct      180

ggcaagggcc tggagtggat cgctcgcatc tatccaggct ctggcaacac ctactataat      240

gagaagttta agggccgggc caccctgaca gctgataaga gcacctctac agcttacatg      300

cagctgtcca gcctgagatc cgaggacacc gccgtgtact tctgcgctcg cggcgtgtac      360

tattttgatt attggggcca gggcaccaca gtgaccgtgt cttccgctag cacaaagggc      420

ccttccgtgt ttccactggc tcccagctct aagtccacca gcggaggaac agccgctctg      480

ggctgtctgg tgaaggacta tttcccagag cccgtgaccg tgagctggaa ctctggcgcc      540

ctgaccagcg gagtgcatac atttcctgct gtgctgcagt ccagcggcct gtactctctg      600

tcttccgtgg tgaccgtgcc aagctcttcc ctgggcaccc agacatatat ctgcaacgtg      660

aatcacaagc catccaatac aaaggtggac aagaaggtgg agcccaagag ctgtgataag      720

acccatacat gccccccttg tcctgctcca gaggctcagg gaggaccatc cgtgttcctg      780

tttccaccca agcctaagga caccctgtac atcacaaggg agccagaggt gacctgcgtg      840

gtggtggacg tgagccacga ggatcccgag gtgaagttca actggtacgt ggatggcgtg      900

gaggtgcata atgccaagac aaagccaagg gaggagcagt acaatagcac ctatcgggtg      960

gtgtctgtgc tgacagtgct gcaccaggac tggctgaacg gcaaggagta caagtgccag     1020

gtgtctaata aggccctgcc cgctcctatc gagaagacca tctccaaggc caagggccag     1080

cctagggagc cacaggtgta cacactgcct ccaagccggg acgagctgac caagaaccag     1140

gtgtctctga catgtctggt gaagggcttc tatccctctg atatcgctgt ggagtgggag     1200

tccaatggcc agcctgagaa caattacaag accacacccc ctgtgctgga ctccgatggc     1260

agcttctttc tgtattccaa gctgaccgtg gataagagca ggtggcagca gggcaacgtg     1320

ttttcttgtt ccgtgatgca tgaggctctg cacaatcatt acacacagaa gagcctgtct     1380

ctgtcccctg gc                                                         1392


<210>  24
<211>  702
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA light chain sequence of an anti-KIT antibody

<400>  24
atgtccgtgc caacccaggt gctgggactg ctgctgctgt ggctgaccga cgccaggtgc       60

gatatcgtga tgacacagtc cccttccagc ctgtctgctt ccgtgggcga cagagtgacc      120

atcacctgta aggccagcca gaacgtgcgc accaatgtgg cttggtacca gcagaagcca      180

ggcaaggccc ccaaggctct gatctatagc gcctcttaca ggtatagcgg agtgcctgac      240

cggttcaccg gatccggaag cggaacagac ttcaccctga caatctcttc cctgcagcct      300

gaggacttcg ctgattactt ttgccagcag tacaactctt atccaaggac cttcggcggc      360

ggcacaaagg tggagatcaa gcggaccgtg gccgctccaa gcgtgttcat ctttccccct      420

tctgacgagc agctgaagtc tggcacagcc tccgtggtgt gcctgctgaa caacttctac      480

cccagagagg ccaaggtgca gtggaaggtg gataacgctc tgcagtctgg caattcccag      540

gagagcgtga ccgagcagga ctctaaggat tccacatata gcctgagctc taccctgaca      600

ctgtctaagg ccgattacga gaagcacaag gtgtatgctt gcgaggtgac ccatcagggc      660

ctgtccagcc cagtgacaaa gtccttcaat cgcggcgagt gt                         702


<210>  25
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain CDR1 of anti-KIT antibody (VH-CDR1)

<400>  25

Gly Tyr Thr Phe Thr Asp Tyr 
1               5           


<210>  26
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain CDR2 of anti-KIT antibody (VH-CDR2)

<400>  26

Tyr Pro Gly Ser Gly Asn 
1               5       


<210>  27
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain CDR3 of anti-KIT antibody (VH-CDR3 - AbM)

<400>  27

Gly Val Tyr Tyr Phe Asp Tyr Trp 
1               5               


<210>  28
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain CDR1 of anti-KIT antibody (VL-CDR1)

<400>  28

Ser Gln Asn Val Arg Thr Asn 
1               5           


<210>  29
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain CDR2 of anti-KIT antibody (VL-CDR2)

<400>  29

Ser Ala Ser 
1           


<210>  30
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain CDR3 of anti-KIT antibody (VL-CDR3)

<400>  30

Tyr Asn Ser Tyr Pro Arg 
1               5       


<210>  31
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain CDR2 of anti-KIT antibody (VH-CDR2)

<400>  31

Pro Gly Ser Gly 
1               


<210>  32
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain CDR3 of anti-KIT antibody (VH-CDR3)

<400>  32

Val Tyr Tyr Phe Asp Tyr 
1               5       


<210>  33
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain CDR1 of anti-KIT antibody (VH-CDR1 - AbM)

<400>  33

Gly Tyr Thr Phe Thr Asp Tyr Tyr Ile Asn 
1               5                   10  


<210>  34
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain CDR2 of anti-KIT antibody (VH-CDR2 - AbM)

<400>  34

Arg Ile Tyr Pro Gly Ser Gly Asn Thr Tyr 
1               5                   10  


<210>  35
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain CDR1 of anti-KIT antibody (VL-CDR1 - Contact)

<400>  35

Arg Thr Asn Val Ala Trp Tyr 
1               5           


<210>  36
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain CDR2 of anti-KIT antibody (VL-CDR2 - Contact)

<400>  36

Ala Leu Ile Tyr Ser Ala Ser Tyr Arg Tyr 
1               5                   10  


<210>  37
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain CDR3 of anti-KIT antibody (VL-CDR3 - Contact)

<400>  37

Gln Gln Tyr Asn Ser Tyr Pro Arg 
1               5               


<210>  38
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain CDR1 of anti-KIT antibody (VH-CDR1 - Contact)

<400>  38

Thr Asp Tyr Tyr Ile Asn 
1               5       


<210>  39
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain CDR2 of anti-KIT antibody (VH-CDR2 - Contact)

<400>  39

Trp Ile Ala Arg Ile Tyr Pro Gly Ser Gly Asn Thr Tyr 
1               5                   10              


<210>  40
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain CDR3 of anti-KIT antibody (VH-CDR3 - Contact)

<400>  40

Ala Arg Gly Val Tyr Tyr Phe Asp Tyr 
1               5                   


