
                                SEQUENCE LISTING

<110> Neogene Therapeutics B.V.
      Schumacher, Antonius Nicolaas Maria
      Linnemann, Carsten
      Kuilman, Thomas
      Bendle, Gavin M.
      Gadiot, Jules F.C.
      van Heijst, Jeroen W.J.
      Gomez-Eerland, Raquel
      Schrikkema, Deborah Sophie
      Walker, Julia Zoe
      Bies, Laura

<120> ENGINEERED ANTIGEN PRESENTING CELLS
  

<130> NTBV.015WO

<150> US 63/139680        
<151> 2020-01-20  

<160> 10

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 1223
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Polypeptide

<400> 1
Met Ala Ser Pro Ala Asp Ser Cys Ile Gln Phe Thr Arg His Ala Ser
 1               5                  10                  15      
Asp Val Leu Leu Asn Leu Asn Arg Leu Arg Ser Arg Asp Ile Leu Thr
            20                  25                  30          
Asp Val Val Ile Val Val Ser Arg Glu Gln Phe Arg Ala His Lys Thr
        35                  40                  45              
Val Leu Met Ala Cys Ser Gly Leu Phe Tyr Ser Ile Phe Thr Asp Gln
    50                  55                  60                  
Leu Lys Cys Asn Leu Ser Val Ile Asn Leu Asp Pro Glu Ile Asn Pro
65                  70                  75                  80  
Glu Gly Phe Cys Ile Leu Leu Asp Phe Met Tyr Thr Ser Arg Leu Asn
                85                  90                  95      
Leu Arg Glu Gly Asn Ile Met Ala Val Met Ala Thr Ala Met Tyr Leu
            100                 105                 110         
Gln Met Glu His Val Val Asp Thr Cys Arg Lys Phe Ile Lys Ala Ser
        115                 120                 125             
Glu Ala Glu Met Val Ser Ala Ile Lys Pro Pro Arg Glu Glu Phe Leu
    130                 135                 140                 
Asn Ser Arg Met Leu Met Pro Gln Asp Ile Met Ala Tyr Arg Gly Arg
145                 150                 155                 160 
Glu Val Val Glu Asn Asn Leu Pro Leu Arg Ser Ala Pro Gly Cys Glu
                165                 170                 175     
Ser Arg Ala Phe Ala Pro Ser Leu Tyr Ser Gly Leu Ser Thr Pro Pro
            180                 185                 190         
Ala Ser Tyr Ser Met Tyr Ser His Leu Pro Val Ser Ser Leu Leu Phe
        195                 200                 205             
Ser Asp Glu Glu Phe Arg Asp Val Arg Met Pro Val Ala Asn Pro Phe
    210                 215                 220                 
Pro Lys Glu Arg Ala Leu Pro Cys Asp Ser Ala Arg Pro Val Pro Gly
225                 230                 235                 240 
Glu Tyr Ser Arg Pro Thr Leu Glu Val Ser Pro Asn Val Cys His Ser
                245                 250                 255     
Asn Ile Tyr Ser Pro Lys Glu Thr Ile Pro Glu Glu Ala Arg Ser Asp
            260                 265                 270         
Met His Tyr Ser Val Ala Glu Gly Leu Lys Pro Ala Ala Pro Ser Ala
        275                 280                 285             
Arg Asn Ala Pro Tyr Phe Pro Cys Asp Lys Ala Ser Lys Glu Glu Glu
    290                 295                 300                 
Arg Pro Ser Ser Glu Asp Glu Ile Ala Leu His Phe Glu Pro Pro Asn
305                 310                 315                 320 
Ala Pro Leu Asn Arg Lys Gly Leu Val Ser Pro Gln Ser Pro Gln Lys
                325                 330                 335     
Ser Asp Cys Gln Pro Asn Ser Pro Thr Glu Ser Cys Ser Ser Lys Asn
            340                 345                 350         
Ala Cys Ile Leu Gln Ala Ser Gly Ser Pro Pro Ala Lys Ser Pro Thr
        355                 360                 365             
Asp Pro Lys Ala Cys Asn Trp Lys Lys Tyr Lys Phe Ile Val Leu Asn
    370                 375                 380                 
Ser Leu Asn Gln Asn Ala Lys Pro Glu Gly Pro Glu Gln Ala Glu Leu
385                 390                 395                 400 
Gly Arg Leu Ser Pro Arg Ala Tyr Thr Ala Pro Pro Ala Cys Gln Pro
                405                 410                 415     
Pro Met Glu Pro Glu Asn Leu Asp Leu Gln Ser Pro Thr Lys Leu Ser
            420                 425                 430         
Ala Ser Gly Glu Asp Ser Thr Ile Pro Gln Ala Ser Arg Leu Asn Asn
        435                 440                 445             
Ile Val Asn Arg Ser Met Thr Gly Ser Pro Arg Ser Ser Ser Glu Ser
    450                 455                 460                 
His Ser Pro Leu Tyr Met His Pro Pro Lys Cys Thr Ser Cys Gly Ser
465                 470                 475                 480 
Gln Ser Pro Gln His Ala Glu Met Cys Leu His Thr Ala Gly Pro Thr
                485                 490                 495     
Phe Pro Glu Glu Met Gly Glu Thr Gln Ser Glu Tyr Ser Asp Ser Ser
            500                 505                 510         
Cys Glu Asn Gly Ala Phe Phe Cys Asn Glu Cys Asp Cys Arg Phe Ser
        515                 520                 525             
Glu Glu Ala Ser Leu Lys Arg His Thr Leu Gln Thr His Ser Asp Lys
    530                 535                 540                 
Pro Tyr Lys Cys Asp Arg Cys Gln Ala Ser Phe Arg Tyr Lys Gly Asn
545                 550                 555                 560 
Leu Ala Ser His Lys Thr Val His Thr Gly Glu Lys Pro Tyr Arg Cys
                565                 570                 575     
Asn Ile Cys Gly Ala Gln Phe Asn Arg Pro Ala Asn Leu Lys Thr His
            580                 585                 590         
Thr Arg Ile His Ser Gly Glu Lys Pro Tyr Lys Cys Glu Thr Cys Gly
        595                 600                 605             
Ala Arg Phe Val Gln Val Ala His Leu Arg Ala His Val Leu Ile His
    610                 615                 620                 
Thr Gly Glu Lys Pro Tyr Pro Cys Glu Ile Cys Gly Thr Arg Phe Arg
625                 630                 635                 640 
His Leu Gln Thr Leu Lys Ser His Leu Arg Ile His Thr Gly Glu Lys
                645                 650                 655     
Pro Tyr His Cys Glu Lys Cys Asn Leu His Phe Arg His Lys Ser Gln
            660                 665                 670         
Leu Arg Leu His Leu Arg Gln Lys His Gly Ala Ile Thr Asn Thr Lys
        675                 680                 685             
Val Gln Tyr Arg Val Ser Ala Thr Asp Leu Pro Pro Glu Leu Pro Lys
    690                 695                 700                 
Ala Cys Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp
705                 710                 715                 720 
Val Glu Glu Asn Pro Gly Pro Met Ser Gln Ser Asn Arg Glu Leu Val
                725                 730                 735     
Val Asp Phe Leu Ser Tyr Lys Leu Ser Gln Lys Gly Tyr Ser Trp Ser
            740                 745                 750         
Gln Phe Ser Asp Val Glu Glu Asn Arg Thr Glu Ala Pro Glu Gly Thr
        755                 760                 765             
Glu Ser Glu Met Glu Thr Pro Ser Ala Ile Asn Gly Asn Pro Ser Trp
    770                 775                 780                 
His Leu Ala Asp Ser Pro Ala Val Asn Gly Ala Thr Gly His Ser Ser
785                 790                 795                 800 
Ser Leu Asp Ala Arg Glu Val Ile Pro Met Ala Ala Val Lys Gln Ala
                805                 810                 815     
Leu Arg Glu Ala Gly Asp Glu Phe Glu Leu Arg Tyr Arg Arg Ala Phe
            820                 825                 830         
Ser Asp Leu Thr Ser Gln Leu His Ile Thr Pro Gly Thr Ala Tyr Gln
        835                 840                 845             
Ser Phe Glu Gln Val Val Asn Glu Leu Phe Arg Asp Gly Val Asn Trp
    850                 855                 860                 
Gly Arg Ile Val Ala Phe Phe Ser Phe Gly Gly Ala Leu Cys Val Glu
865                 870                 875                 880 
Ser Val Asp Lys Glu Met Gln Val Leu Val Ser Arg Ile Ala Ala Trp
                885                 890                 895     
Met Ala Thr Tyr Leu Asn Asp His Leu Glu Pro Trp Ile Gln Glu Asn
            900                 905                 910         
Gly Gly Trp Asp Thr Phe Val Glu Leu Tyr Gly Asn Asn Ala Ala Ala
        915                 920                 925             
Glu Ser Arg Lys Gly Gln Glu Arg Phe Asn Arg Trp Phe Leu Thr Gly
    930                 935                 940                 
Met Thr Val Ala Gly Val Val Leu Leu Gly Ser Leu Phe Ser Arg Lys
945                 950                 955                 960 
Glu Phe Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly
                965                 970                 975     
Asp Val Glu Glu Asn Pro Gly Pro Met Val Ser Lys Gly Glu Glu Leu
            980                 985                 990         
Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn
        995                 1000                1005            
Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr
    1010                1015                1020                
Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val
1025                1030                1035               1040 
Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly Val Gln Cys Phe
                1045                1050                1055    
Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala
            1060                1065                1070        
Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp
        1075                1080                1085            
Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu
    1090                1095                1100                
Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn
1105                1110                1115               1120 
Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr
                1125                1130                1135    
Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys Ile
            1140                1145                1150        
Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln
        1155                1160                1165            
Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His
    1170                1175                1180                
Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg
1185                1190                1195               1200 
Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu
                1205                1210                1215    
Gly Met Asp Glu Leu Tyr Lys
            1220            


<210> 2
<211> 467
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Polypeptide

<400> 2
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu
 1               5                  10                  15      
His Ala Ala Arg Pro Ser Gln Phe Arg Val Ser Pro Leu Asp Arg Thr
            20                  25                  30          
Trp Asn Leu Gly Glu Thr Val Glu Leu Lys Cys Gln Val Leu Leu Ser
        35                  40                  45              
Asn Pro Thr Ser Gly Cys Ser Trp Leu Phe Gln Pro Arg Gly Ala Ala
    50                  55                  60                  
Ala Ser Pro Thr Phe Leu Leu Tyr Leu Ser Gln Asn Lys Pro Lys Ala
65                  70                  75                  80  
Ala Glu Gly Leu Asp Thr Gln Arg Phe Ser Gly Lys Arg Leu Gly Asp
                85                  90                  95      
Thr Phe Val Leu Thr Leu Ser Asp Phe Arg Arg Glu Asn Glu Gly Tyr
            100                 105                 110         
Tyr Phe Cys Ser Ala Leu Ser Asn Ser Ile Met Tyr Phe Ser His Phe
        115                 120                 125             
Val Pro Val Phe Leu Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg
    130                 135                 140                 
Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg
145                 150                 155                 160 
Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly
                165                 170                 175     
Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr
            180                 185                 190         
Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His
        195                 200                 205             
Arg Asn Arg Arg Arg Val Cys Lys Cys Pro Arg Pro Val Val Lys Ser
    210                 215                 220                 
Gly Asp Lys Pro Ser Leu Ser Ala Arg Tyr Val Gly Ser Gly Ala Thr
225                 230                 235                 240 
Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly
                245                 250                 255     
Pro Met Arg Pro Arg Leu Trp Leu Leu Leu Ala Ala Gln Leu Thr Val
            260                 265                 270         
Leu His Gly Asn Ser Val Leu Gln Gln Thr Pro Ala Tyr Ile Lys Val
        275                 280                 285             
Gln Thr Asn Lys Met Val Met Leu Ser Cys Glu Ala Lys Ile Ser Leu
    290                 295                 300                 
Ser Asn Met Arg Ile Tyr Trp Leu Arg Gln Arg Gln Ala Pro Ser Ser
305                 310                 315                 320 
Asp Ser His His Glu Phe Leu Ala Leu Trp Asp Ser Ala Lys Gly Thr
                325                 330                 335     
Ile His Gly Glu Glu Val Glu Gln Glu Lys Ile Ala Val Phe Arg Asp
            340                 345                 350         
Ala Ser Arg Phe Ile Leu Asn Leu Thr Ser Val Lys Pro Glu Asp Ser
        355                 360                 365             
Gly Ile Tyr Phe Cys Met Ile Val Gly Ser Pro Glu Leu Thr Phe Gly
    370                 375                 380                 
Lys Gly Thr Gln Leu Ser Val Val Asp Phe Leu Pro Thr Thr Ala Gln
385                 390                 395                 400 
Pro Thr Lys Lys Ser Thr Leu Lys Lys Arg Val Cys Arg Leu Pro Arg
                405                 410                 415     
Pro Glu Thr Gln Lys Gly Pro Leu Cys Ser Pro Ile Thr Leu Gly Leu
            420                 425                 430         
Leu Val Ala Gly Val Leu Val Leu Leu Val Ser Leu Gly Val Ala Ile
        435                 440                 445             
His Leu Cys Cys Arg Arg Arg Arg Ala Arg Leu Arg Phe Met Lys Gln
    450                 455                 460                 
Phe Tyr Lys
465         


<210> 3
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Polypeptide

<400> 3
Ser Leu Leu Met Trp Ile Thr Gln Cys Phe Leu Pro Val Phe
 1               5                  10                  


<210> 4
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Polypeptide

<400> 4
Ser Leu Leu Met Trp Ile Thr Gln Cys
 1               5                  


<210> 5
<211> 10
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Polypeptide

<400> 5
Ala Leu Leu Glu Thr Pro Ser Leu Leu Leu
 1               5                  10  


<210> 6
<211> 25
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Polypeptide

<400> 6
Phe Ser Trp Asn Val Lys Ala Ala Leu Leu Glu Thr Pro Ser Leu Leu
 1               5                  10                  15      
Leu Ala Lys Val Gly Ile Ala Leu Lys
            20                  25  


<210> 7
<211> 25
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Polypeptide

<400> 7
Trp Gln Ala Gly Ile Leu Ala Arg Asn Leu Val Pro Met Val Ala Thr
 1               5                  10                  15      
Val Gln Gly Gln Asn Leu Lys Tyr Gln
            20                  25  


<210> 8
<211> 25
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Polypeptide

<400> 8
Ile Leu Gly Asp Pro Lys Lys Leu Leu Thr Gln His Phe Val Gln Glu
 1               5                  10                  15      
Asn Tyr Leu Glu Tyr Arg Gln Val Pro
            20                  25  


<210> 9
<211> 261
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Polypeptide

<400> 9
Met Ile Glu Thr Tyr Asn Gln Thr Ser Pro Arg Ser Ala Ala Thr Gly
 1               5                  10                  15      
Leu Pro Ile Ser Met Lys Ile Phe Met Tyr Leu Leu Thr Val Phe Leu
            20                  25                  30          
Ile Thr Gln Met Ile Gly Ser Ala Leu Phe Ala Val Tyr Leu His Arg
        35                  40                  45              
Arg Leu Asp Lys Ile Glu Asp Glu Arg Asn Leu His Glu Asp Phe Val
    50                  55                  60                  
Phe Met Lys Thr Ile Gln Arg Cys Asn Thr Gly Glu Arg Ser Leu Ser
65                  70                  75                  80  
Leu Leu Asn Cys Glu Glu Ile Lys Ser Gln Phe Glu Gly Phe Val Lys
                85                  90                  95      
Asp Ile Met Leu Asn Lys Glu Glu Thr Lys Lys Glu Asn Ser Phe Glu
            100                 105                 110         
Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser
        115                 120                 125             
Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly
    130                 135                 140                 
Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln
145                 150                 155                 160 
Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr
                165                 170                 175     
Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser
            180                 185                 190         
Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala
        195                 200                 205             
Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His
    210                 215                 220                 
Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn
225                 230                 235                 240 
Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe
                245                 250                 255     
Gly Leu Leu Lys Leu
            260     


<210> 10
<211> 261
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic Polypeptide

<400> 10
Met Ile Glu Thr Tyr Asn Gln Thr Ser Pro Arg Ser Ala Ala Thr Gly
 1               5                  10                  15      
Leu Pro Ile Ser Met Lys Ile Phe Met Tyr Leu Leu Thr Val Phe Leu
            20                  25                  30          
Ile Thr Gln Met Ile Gly Ser Ala Leu Phe Ala Val Tyr Leu His Arg
        35                  40                  45              
Arg Leu Asp Lys Ile Glu Asp Glu Arg Asn Leu His Glu Asp Phe Val
    50                  55                  60                  
Phe Met Lys Thr Ile Gln Arg Cys Asn Thr Gly Glu Arg Ser Leu Ser
65                  70                  75                  80  
Leu Leu Asn Cys Glu Glu Ile Lys Ser Gln Phe Glu Gly Phe Val Lys
                85                  90                  95      
Asp Ile Met Leu Asn Lys Glu Glu Thr Lys Lys Glu Asn Ser Phe Glu
            100                 105                 110         
Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser
        115                 120                 125             
Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly
    130                 135                 140                 
Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln
145                 150                 155                 160 
Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr
                165                 170                 175     
Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser
            180                 185                 190         
Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala
        195                 200                 205             
Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His
    210                 215                 220                 
Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn
225                 230                 235                 240 
Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe
                245                 250                 255     
Gly Leu Leu Lys Leu
            260     

