                         SEQUENCE LISTING

<110>  KAHR Medical Ltd.
       Thomas Jefferson University
       TAMIR, Ami
       TYKOCINSKI, Mark L.
       BREMER, Edwin
 
<120>  TYPE I MEMBRANE PROTEINS HETERODIMERS AND METHODS OF USE THEREOF

<130>  89962

<150>  US 63/136,687
<151>  2021-01-13

<150>  US 63/139,331
<151>  2021-01-20

<160>  164   

<170>  PatentIn version 3.5

<210>  1
<211>  585
<212>  PRT
<213>  Artificial sequence

<220>
<223>  SIRP Fc(IgG1 knob) 585AA first monomer of DSP120, DSP216, DSP403,
       DSP503

<400>  1

Glu Glu Glu Leu Gln Val Ile Gln Pro Asp Lys Ser Val Leu Val Ala 
1               5                   10                  15      


Ala Gly Glu Thr Ala Thr Leu Arg Cys Thr Ala Thr Ser Leu Ile Pro 
            20                  25                  30          


Val Gly Pro Ile Gln Trp Phe Arg Gly Ala Gly Pro Gly Arg Glu Leu 
        35                  40                  45              


Ile Tyr Asn Gln Lys Glu Gly His Phe Pro Arg Val Thr Thr Val Ser 
    50                  55                  60                  


Asp Leu Thr Lys Arg Asn Asn Met Asp Phe Ser Ile Arg Ile Gly Asn 
65                  70                  75                  80  


Ile Thr Pro Ala Asp Ala Gly Thr Tyr Tyr Cys Val Lys Phe Arg Lys 
                85                  90                  95      


Gly Ser Pro Asp Asp Val Glu Phe Lys Ser Gly Ala Gly Thr Glu Leu 
            100                 105                 110         


Ser Val Arg Ala Lys Pro Ser Ala Pro Val Val Ser Gly Pro Ala Ala 
        115                 120                 125             


Arg Ala Thr Pro Gln His Thr Val Ser Phe Thr Cys Glu Ser His Gly 
    130                 135                 140                 


Phe Ser Pro Arg Asp Ile Thr Leu Lys Trp Phe Lys Asn Gly Asn Glu 
145                 150                 155                 160 


Leu Ser Asp Phe Gln Thr Asn Val Asp Pro Val Gly Glu Ser Val Ser 
                165                 170                 175     


Tyr Ser Ile His Ser Thr Ala Lys Val Val Leu Thr Arg Glu Asp Val 
            180                 185                 190         


His Ser Gln Val Ile Cys Glu Val Ala His Val Thr Leu Gln Gly Asp 
        195                 200                 205             


Pro Leu Arg Gly Thr Ala Asn Leu Ser Glu Thr Ile Arg Val Pro Pro 
    210                 215                 220                 


Thr Leu Glu Val Thr Gln Gln Pro Val Arg Ala Glu Asn Gln Val Asn 
225                 230                 235                 240 


Val Thr Cys Gln Val Arg Lys Phe Tyr Pro Gln Arg Leu Gln Leu Thr 
                245                 250                 255     


Trp Leu Glu Asn Gly Asn Val Ser Arg Thr Glu Thr Ala Ser Thr Val 
            260                 265                 270         


Thr Glu Asn Lys Asp Gly Thr Tyr Asn Trp Met Ser Trp Leu Leu Val 
        275                 280                 285             


Asn Val Ser Ala His Arg Asp Asp Val Lys Leu Thr Cys Gln Val Glu 
    290                 295                 300                 


His Asp Gly Gln Pro Ala Val Ser Lys Ser His Asp Leu Lys Val Ser 
305                 310                 315                 320 


Ala His Pro Lys Glu Gln Gly Ser Asn Thr Ala Ala Glu Asn Thr Gly 
                325                 330                 335     


Ser Asn Glu Arg Asn Ile Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly 
            340                 345                 350         


Ser Glu Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
        355                 360                 365             


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
    370                 375                 380                 


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
385                 390                 395                 400 


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
                405                 410                 415     


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
            420                 425                 430         


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
        435                 440                 445             


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
    450                 455                 460                 


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
465                 470                 475                 480 


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Cys Arg Asp Glu Leu 
                485                 490                 495     


Thr Lys Asn Gln Val Ser Leu Trp Cys Leu Val Lys Gly Phe Tyr Pro 
            500                 505                 510         


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
        515                 520                 525             


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
    530                 535                 540                 


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
545                 550                 555                 560 


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
                565                 570                 575     


Lys Ser Leu Ser Leu Ser Pro Gly Lys 
            580                 585 


<210>  2
<211>  1755
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA of #1

<400>  2
gaggaggagc tgcaggtcat ccagcccgat aagtctgtgc tggtggcagc aggagagacc       60

gccacactga ggtgcaccgc cacaagcctg atcccagtgg gaccaatcca gtggtttagg      120

ggagcaggcc ctggcagaga gctgatctac aaccagaagg agggccactt cccaagagtg      180

accacagtga gcgacctgac caagcggaac aatatggatt tttccatcag aatcggcaat      240

atcacacctg ccgacgccgg cacctactat tgcgtgaagt tcaggaaggg ctccccagac      300

gatgtggagt ttaagagcgg agcaggcacc gagctgtccg tgcgggcaaa gccttccgcc      360

ccagtggtgt ctggaccagc agccagagcc accccacagc acacagtgtc cttcacctgt      420

gagtctcacg gctttagccc ccgggacatc accctgaagt ggttcaagaa cggcaatgag      480

ctgtctgact ttcagaccaa cgtggacccc gtgggcgagt ctgtgagcta ttccatccac      540

tctacagcca aggtggtgct gacccgcgag gacgtgcaca gccaggtcat ctgcgaggtg      600

gcacacgtga ccctgcaggg cgatcctctg aggggcacag ccaatctgag cgagaccatc      660

agagtgcccc ctacactgga ggtgacccag cagcccgtgc gcgcagagaa ccaagtgaat      720

gtgacatgtc aggtgaggaa gttctaccct cagcgcctgc agctgacctg gctggagaac      780

ggcaacgtga gccggaccga gacagccagc accgtgacag agaacaagga cggcacatat      840

aattggatgt cttggctgct ggtgaacgtg agcgcccaca gggacgatgt gaagctgacc      900

tgccaggtgg agcacgacgg acagccagcc gtgtctaaga gccacgatct gaaggtgagc      960

gcccacccta aggagcaggg ctccaacaca gccgccgaga ataccggcag caacgagcgg     1020

aatatctacg gaggaggagg cagcggagga ggaggctccg agcctaagag ctccgacaag     1080

acccacacat gcccaccatg tcctgcacca gagctgctgg gaggaccttc cgtgttcctg     1140

tttcctccaa agccaaagga tacactgatg atctccagaa caccagaggt gacctgcgtg     1200

gtggtggacg tgtctcacga ggaccccgag gtgaagttta actggtacgt ggacggcgtg     1260

gaggtgcaca atgccaagac caagccaagg gaggagcagt acaactccac atatcgcgtg     1320

gtgtctgtgc tgaccgtgct gcaccaggat tggctgaacg gcaaggagta taagtgtaag     1380

gtgagcaata aggccctgcc cgcccctatc gagaagacca tctccaaggc aaagggacag     1440

cccagggagc ctcaggtgta cacactgccc ccttgccgcg acgagctgac caagaaccag     1500

gtgtctctgt ggtgtctggt gaagggcttc tacccatctg acatcgccgt ggagtgggag     1560

agcaatggcc agcccgagaa caattacaag accacaccac ccgtgctgga cagcgatggc     1620

tccttctttc tgtattccaa gctgacagtg gacaagtctc ggtggcagca gggcaacgtg     1680

ttttcctgtt ctgtgatgca cgaggccctg cacaatcact atacccagaa gagcctgtcc     1740

ctgtctcccg gcaag                                                      1755


<210>  3
<211>  382
<212>  PRT
<213>  Artificial sequence

<220>
<223>  PD1 Fc (IgG1 hole) 382 AA second monomer of DSP120, DSP220, 
       DSP402, DSP502

<400>  3

Asp Ser Pro Asp Arg Pro Trp Asn Pro Pro Thr Phe Ser Pro Ala Leu 
1               5                   10                  15      


Leu Val Val Thr Glu Gly Asp Asn Ala Thr Phe Thr Cys Ser Phe Ser 
            20                  25                  30          


Asn Thr Ser Glu Ser Phe Val Leu Asn Trp Tyr Arg Met Ser Pro Ser 
        35                  40                  45              


Asn Gln Thr Asp Lys Leu Ala Ala Phe Pro Glu Asp Arg Ser Gln Pro 
    50                  55                  60                  


Gly Gln Asp Ser Arg Phe Arg Val Thr Gln Leu Pro Asn Gly Arg Asp 
65                  70                  75                  80  


Phe His Met Ser Val Val Arg Ala Arg Arg Asn Asp Ser Gly Thr Tyr 
                85                  90                  95      


Leu Cys Gly Ala Ile Ser Leu Ala Pro Lys Ala Gln Ile Lys Glu Ser 
            100                 105                 110         


Leu Arg Ala Glu Leu Arg Val Thr Glu Arg Arg Ala Glu Val Pro Thr 
        115                 120                 125             


Ala His Pro Ser Pro Ser Pro Arg Pro Ala Gly Gln Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Glu Pro Lys Ser Ser Asp Lys Thr His Thr 
145                 150                 155                 160 


Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 
                165                 170                 175     


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
            180                 185                 190         


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
        195                 200                 205             


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
    210                 215                 220                 


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
225                 230                 235                 240 


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
                245                 250                 255     


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
            260                 265                 270         


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Cys Thr Leu Pro Pro 
        275                 280                 285             


Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Ser Cys Ala Val 
    290                 295                 300                 


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
305                 310                 315                 320 


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
                325                 330                 335     


Gly Ser Phe Phe Leu Val Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
            340                 345                 350         


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
        355                 360                 365             


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    370                 375                 380         


<210>  4
<211>  1146
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA of# 3

<400>  4
gattcacccg atagaccttg gaacccacct accttctccc ccgccctgct ggtggtgaca       60

gagggcgaca atgccacctt cacatgctct tttagcaaca cctccgagtc tttcgtgctg      120

aattggtaca ggatgagccc ctccaaccag acagataagc tggccgcatt tccagaggac      180

cgcagccagc caggacagga ttcccggttc agagtgaccc agctgcctaa tggccgggac      240

tttcacatgt ctgtggtgag agcccggaga aacgatagcg gcacatacct gtgcggagcc      300

atctccctgg cccctaaggc acagatcaag gagtccctga gggcagagct gagggtgacc      360

gagaggaggg cagaggtgcc aacagcacac ccttctccaa gcccccggcc tgcaggacag      420

ggaggaggag gctccggcgg cggcggctct gagccaaaga gctccgacaa gacccacaca      480

tgcccaccat gtccagcacc agagctgctg ggaggaccta gcgtgttcct gtttcctcca      540

aagccaaagg ataccctgat gatctctagg accccagagg tgacatgcgt ggtggtggac      600

gtgagccacg aggaccccga ggtgaagttt aattggtacg tggacggcgt ggaggtgcac      660

aacgccaaga caaagcctag ggaggagcag tacaattcta cctatcgcgt ggtgagcgtg      720

ctgacagtgc tgcaccagga ttggctgaat ggcaaggagt ataagtgtaa ggtgtccaac      780

aaggccctgc ctgccccaat cgagaagacc atctctaagg caaagggaca gccccgggag      840

cctcaggtgt gcaccctgcc ccctagcaga gacgagctga caaagaatca ggtgtccctg      900

tcttgtgccg tgaagggctt ctaccccagc gacatcgcag tggagtggga gtccaacgga      960

cagcctgaga acaattataa gaccacacca cccgtgctgg actctgatgg cagcttcttt     1020

ctggtgtcca agctgaccgt ggacaagtct cggtggcagc agggcaacgt gtttagctgc     1080

tccgtgatgc acgaagcact gcacaaccac tacacccaga agtcactgtc actgtcccca     1140

ggaaag                                                                1146


<210>  5
<211>  582
<212>  PRT
<213>  Artificial sequence

<220>
<223>  SIRP alpha (IgG4 knob)  582 AA first monomer of DSP120V1, 
       DSP216V1, DSP403V1, DSP503V1

<400>  5

Glu Glu Glu Leu Gln Val Ile Gln Pro Asp Lys Ser Val Leu Val Ala 
1               5                   10                  15      


Ala Gly Glu Thr Ala Thr Leu Arg Cys Thr Ala Thr Ser Leu Ile Pro 
            20                  25                  30          


Val Gly Pro Ile Gln Trp Phe Arg Gly Ala Gly Pro Gly Arg Glu Leu 
        35                  40                  45              


Ile Tyr Asn Gln Lys Glu Gly His Phe Pro Arg Val Thr Thr Val Ser 
    50                  55                  60                  


Asp Leu Thr Lys Arg Asn Asn Met Asp Phe Ser Ile Arg Ile Gly Asn 
65                  70                  75                  80  


Ile Thr Pro Ala Asp Ala Gly Thr Tyr Tyr Cys Val Lys Phe Arg Lys 
                85                  90                  95      


Gly Ser Pro Asp Asp Val Glu Phe Lys Ser Gly Ala Gly Thr Glu Leu 
            100                 105                 110         


Ser Val Arg Ala Lys Pro Ser Ala Pro Val Val Ser Gly Pro Ala Ala 
        115                 120                 125             


Arg Ala Thr Pro Gln His Thr Val Ser Phe Thr Cys Glu Ser His Gly 
    130                 135                 140                 


Phe Ser Pro Arg Asp Ile Thr Leu Lys Trp Phe Lys Asn Gly Asn Glu 
145                 150                 155                 160 


Leu Ser Asp Phe Gln Thr Asn Val Asp Pro Val Gly Glu Ser Val Ser 
                165                 170                 175     


Tyr Ser Ile His Ser Thr Ala Lys Val Val Leu Thr Arg Glu Asp Val 
            180                 185                 190         


His Ser Gln Val Ile Cys Glu Val Ala His Val Thr Leu Gln Gly Asp 
        195                 200                 205             


Pro Leu Arg Gly Thr Ala Asn Leu Ser Glu Thr Ile Arg Val Pro Pro 
    210                 215                 220                 


Thr Leu Glu Val Thr Gln Gln Pro Val Arg Ala Glu Asn Gln Val Asn 
225                 230                 235                 240 


Val Thr Cys Gln Val Arg Lys Phe Tyr Pro Gln Arg Leu Gln Leu Thr 
                245                 250                 255     


Trp Leu Glu Asn Gly Asn Val Ser Arg Thr Glu Thr Ala Ser Thr Val 
            260                 265                 270         


Thr Glu Asn Lys Asp Gly Thr Tyr Asn Trp Met Ser Trp Leu Leu Val 
        275                 280                 285             


Asn Val Ser Ala His Arg Asp Asp Val Lys Leu Thr Cys Gln Val Glu 
    290                 295                 300                 


His Asp Gly Gln Pro Ala Val Ser Lys Ser His Asp Leu Lys Val Ser 
305                 310                 315                 320 


Ala His Pro Lys Glu Gln Gly Ser Asn Thr Ala Ala Glu Asn Thr Gly 
                325                 330                 335     


Ser Asn Glu Arg Asn Ile Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly 
            340                 345                 350         


Ser Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu 
        355                 360                 365             


Phe Glu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
    370                 375                 380                 


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
385                 390                 395                 400 


Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly 
                405                 410                 415     


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn 
            420                 425                 430         


Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
        435                 440                 445             


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro 
    450                 455                 460                 


Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 
465                 470                 475                 480 


Pro Gln Val Cys Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn 
                485                 490                 495     


Gln Val Ser Leu Trp Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
            500                 505                 510         


Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
        515                 520                 525             


Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg 
    530                 535                 540                 


Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys 
545                 550                 555                 560 


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
                565                 570                 575     


Ser Leu Ser Leu Gly Lys 
            580         


<210>  6
<211>  1746
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA of#5

<400>  6
gaggaggagc tgcaggtcat ccagcccgat aagtctgtgc tggtggcagc aggagagacc       60

gccacactga gatgcaccgc cacaagcctg atcccagtgg gaccaatcca gtggtttagg      120

ggagcaggac ctggaaggga gctgatctac aaccagaagg agggccactt cccaagggtg      180

accacagtgt ccgacctgac caagcggaac aatatggatt tttctatcag aatcggcaat      240

atcacacctg ccgacgccgg cacctactat tgcgtgaagt tcagaaaggg cagcccagac      300

gatgtggagt ttaagtccgg agcaggaacc gagctgtctg tgagagcaaa gcctagcgcc      360

ccagtggtgt ccggaccagc agcaagggca accccacagc acacagtgtc cttcacctgt      420

gagtcccacg gcttttctcc acgcgatatc acactgaagt ggttcaagaa cggcaatgag      480

ctgagcgact ttcagaccaa cgtggatccc gtgggcgagt ctgtgagcta ctccatccac      540

tctacagcca aggtggtgct gacccgggag gacgtgcaca gccaggtcat ctgcgaggtg      600

gcacacgtga ccctgcaggg cgatcctctg agaggcacag ccaatctgtc cgagaccatc      660

agggtgcccc ctacactgga ggtgacccag cagcccgtga gggcagagaa ccaagtgaat      720

gtgacatgtc aggtgcggaa gttctaccct cagagactgc agctgacctg gctggagaac      780

ggcaatgtga gccgcaccga gacagcctcc accgtgacag agaacaagga cggcacatat      840

aattggatga gctggctgct ggtgaacgtg tccgcccaca gggacgatgt gaagctgacc      900

tgccaggtgg agcacgacgg acagccagcc gtgtctaaga gccacgatct gaaggtgtcc      960

gcccacccta aggagcaggg ctctaacaca gccgccgaga ataccggcag caacgagaga     1020

aatatctacg gaggaggagg atccggagga ggaggatccg agtctaagta tggaccacca     1080

tgccctccat gtccagcacc tgagtttgag ggaggaccta gcgtgttcct gtttccccct     1140

aagccaaagg acacactgat gatctccagg acaccagagg tgacctgcgt ggtggtggac     1200

gtgtctcagg aggatcccga ggtgcagttc aactggtacg tggatggcgt ggaggtgcac     1260

aatgccaaga ccaagcctag ggaggagcag tttaactcta cataccgcgt ggtgagcgtg     1320

ctgaccgtgc tgcaccagga ttggctgaac ggcaaggagt ataagtgtaa ggtgagcaat     1380

aagggcctgc caagctccat cgagaagacc atctccaagg caaagggaca gccaagggag     1440

cctcaggtgt gcacactgcc accctctcag gaggagatga ccaagaacca ggtgagcctg     1500

tggtgtctgg tgaagggctt ctacccaagc gacatcgccg tggagtggga gtccaatggc     1560

cagcccgaga acaattacaa gaccacacct ccagtgctgg actctgatgg cagcttcttt     1620

ctgtattcta ggctgacagt ggataagagc cgctggcagg agggcaacgt gtttagctgt     1680

tccgtgatgc acgaggccct gcacaatcac tatacccaga agtctctgag cctgtccctg     1740

ggcaag                                                                1746


<210>  7
<211>  379
<212>  PRT
<213>  Artificial sequence

<220>
<223>  PD1 (IgG4 hole) 379 AA Second monomer of DSP120V1, DSP402V1, 
       DSP502V2, DSP502V3

<400>  7

Asp Ser Pro Asp Arg Pro Trp Asn Pro Pro Thr Phe Ser Pro Ala Leu 
1               5                   10                  15      


Leu Val Val Thr Glu Gly Asp Asn Ala Thr Phe Thr Cys Ser Phe Ser 
            20                  25                  30          


Asn Thr Ser Glu Ser Phe Val Leu Asn Trp Tyr Arg Met Ser Pro Ser 
        35                  40                  45              


Asn Gln Thr Asp Lys Leu Ala Ala Phe Pro Glu Asp Arg Ser Gln Pro 
    50                  55                  60                  


Gly Gln Asp Ser Arg Phe Arg Val Thr Gln Leu Pro Asn Gly Arg Asp 
65                  70                  75                  80  


Phe His Met Ser Val Val Arg Ala Arg Arg Asn Asp Ser Gly Thr Tyr 
                85                  90                  95      


Leu Cys Gly Ala Ile Ser Leu Ala Pro Lys Ala Gln Ile Lys Glu Ser 
            100                 105                 110         


Leu Arg Ala Glu Leu Arg Val Thr Glu Arg Arg Ala Glu Val Pro Thr 
        115                 120                 125             


Ala His Pro Ser Pro Ser Pro Arg Pro Ala Gly Gln Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro 
145                 150                 155                 160 


Cys Pro Ala Pro Glu Phe Glu Gly Gly Pro Ser Val Phe Leu Phe Pro 
                165                 170                 175     


Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr 
            180                 185                 190         


Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn 
        195                 200                 205             


Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg 
    210                 215                 220                 


Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val 
225                 230                 235                 240 


Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser 
                245                 250                 255     


Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys 
            260                 265                 270         


Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Cys 
        275                 280                 285             


Glu Met Thr Lys Asn Gln Val Ser Leu Ser Cys Ala Val Lys Gly Phe 
    290                 295                 300                 


Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 
305                 310                 315                 320 


Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 
                325                 330                 335     


Phe Leu Val Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly 
            340                 345                 350         


Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr 
        355                 360                 365             


Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys 
    370                 375                 


<210>  8
<211>  1137
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA of#7

<400>  8
gactctccag ataggccttg gaatccccct acctttagcc ccgccctgct ggtggtgaca       60

gagggcgata acgccacctt cacatgctct tttagcaaca cctccgagtc tttcgtgctg      120

aattggtaca ggatgagccc ttccaaccag acagacaagc tggcagcatt tcctgaggac      180

cgcagccagc caggacagga ttcccggttc agagtgaccc agctgccaaa tggcagggac      240

tttcacatgt ctgtggtgcg cgcccggaga aacgatagcg gcacatacct gtgcggagca      300

atctccctgg caccaaaggc acagatcaag gagtccctga gggcagagct gagggtgacc      360

gagaggaggg ccgaggtgcc aacagcacac ccatctccta gcccaaggcc agcaggacag      420

ggaggaggag gctctggagg aggaggatcc gagtctaagt acggaccacc atgccctcca      480

tgtcctgcac cagagttcga gggaggacca tccgtgttcc tgtttccacc taagcctaag      540

gacaccctga tgatctccag aacccccgag gtgacatgcg tggtggtgga cgtgtctcag      600

gaggatcctg aggtgcagtt caattggtac gtggatggcg tggaggtgca caacgccaag      660

acaaagcccc gggaggagca gtttaattct acctacagag tggtgagcgt gctgacagtg      720

ctgcaccagg attggctgaa tggcaaggag tataagtgta aggtgagcaa caagggcctg      780

cctagctcca tcgagaagac catctccaag gccaagggcc agccaagaga gccccaggtg      840

tacaccctgc cacccagcca gtgcgagatg acaaagaatc aggtgagcct gtcctgtgcc      900

gtgaagggct tctaccctag cgacatcgca gtggagtggg agtccaacgg acagccagag      960

aacaattata agaccacacc tccagtgctg gactccgatg gctctttctt tctggtgtcc     1020

cggctgaccg tggataagag ccggtggcag gagggcaacg tgttcagctg cagcgtgatg     1080

cacgaggccc tgcacaacca ctatacacag aagtccctgt ctctgagcct gggcaag        1137


<210>  9
<211>  354
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TIGIT Fc (IgG1 Knob) 354 AA first monomer of DSP205, DSP404, 
       DSP502

<400>  9

Met Thr Gly Thr Ile Glu Thr Thr Gly Asn Ile Ser Ala Glu Lys Gly 
1               5                   10                  15      


Gly Ser Ile Ala Leu Gln Cys His Leu Ser Ser Thr Thr Ala Gln Val 
            20                  25                  30          


Thr Gln Val Asn Trp Glu Gln Gln Asp Gln Leu Leu Ala Ile Ser Asn 
        35                  40                  45              


Ala Asp Leu Gly Trp His Ile Ser Pro Ser Phe Lys Asp Arg Val Ala 
    50                  55                  60                  


Pro Gly Pro Gly Leu Gly Leu Thr Leu Gln Ser Leu Thr Val Asn Asp 
65                  70                  75                  80  


Thr Gly Glu Tyr Phe Cys Ile Tyr His Thr Tyr Pro Asp Gly Thr Tyr 
                85                  90                  95      


Thr Gly Arg Ile Phe Leu Glu Val Leu Glu Ser Ser Val Ala Glu His 
            100                 105                 110         


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Pro Lys Ser Ser Asp 
        115                 120                 125             


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly 
    130                 135                 140                 


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
145                 150                 155                 160 


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
                165                 170                 175     


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
            180                 185                 190         


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 
        195                 200                 205             


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
    210                 215                 220                 


Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
225                 230                 235                 240 


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
                245                 250                 255     


Thr Leu Pro Pro Cys Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu 
            260                 265                 270         


Trp Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
        275                 280                 285             


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
    290                 295                 300                 


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
305                 310                 315                 320 


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
                325                 330                 335     


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
            340                 345                 350         


Gly Lys 
        


<210>  10
<211>  1062
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA of#9

<400>  10
atgaccggca caatcgagac aacaggcaac atctctgccg agaagggagg cagcatcgcc       60

ctgcagtgcc acctgagcag caccacagcc caggtgaccc aggtgaactg ggagcagcag      120

gaccagctgc tggccatctc taatgccgat ctgggctggc acatcagccc atcctttaag      180

gatagggtgg caccaggacc aggcctgggc ctgaccctgc agagcctgac cgtgaatgac      240

acaggcgagt acttctgtat ctaccacaca tatcccgatg gcacctatac aggcagaatc      300

tttctggagg tgctggagtc tagcgtggcc gagcacggag gaggaggcag cggaggagga      360

ggctccgagc ctaagtcctc tgacaagacc cacacatgcc ccccttgtcc tgcaccagag      420

ctgctgggcg gaccttccgt gttcctgttt ccacccaagc caaaggatac cctgatgatc      480

tccaggaccc ctgaggtgac atgcgtggtg gtggacgtgt ctcacgagga ccccgaggtg      540

aagttcaact ggtacgtgga cggcgtggag gtgcacaatg ccaagacaaa gcctcgggag      600

gagcagtaca actccaccta tagagtggtg tctgtgctga cagtgctgca ccaggattgg      660

ctgaacggca aggagtataa gtgtaaggtg agcaataagg ccctgcccgc ccctatcgag      720

aaaaccatca gcaaggcaaa gggacagcca agggagccac aggtgtacac cctgcctcca      780

tgccgcgacg agctgacaaa gaaccaggtg agcctgtggt gtctggtgaa gggcttctat      840

ccatctgaca tcgccgtgga gtgggagagc aatggccagc ccgagaacaa ttacaagacc      900

acaccccctg tgctggactc cgatggctct ttctttctgt atagcaagct gaccgtggac      960

aagtccagat ggcagcaggg caacgtgttt tcttgcagcg tgatgcacga ggccctgcac     1020

aatcactaca cacagaagtc cctgtctctg agccccggca ag                        1062


<210>  11
<211>  645
<212>  PRT
<213>  Artificial sequence

<220>
<223>  LILRB Fc (IgG1 hole) 645 AA second  monomer of DSP205, DSP216, 
       DSP412

<400>  11

Gln Thr Gly Thr Ile Pro Lys Pro Thr Leu Trp Ala Glu Pro Asp Ser 
1               5                   10                  15      


Val Ile Thr Gln Gly Ser Pro Val Thr Leu Ser Cys Gln Gly Ser Leu 
            20                  25                  30          


Glu Ala Gln Glu Tyr Arg Leu Tyr Arg Glu Lys Lys Ser Ala Ser Trp 
        35                  40                  45              


Ile Thr Arg Ile Arg Pro Glu Leu Val Lys Asn Gly Gln Phe His Ile 
    50                  55                  60                  


Pro Ser Ile Thr Trp Glu His Thr Gly Arg Tyr Gly Cys Gln Tyr Tyr 
65                  70                  75                  80  


Ser Arg Ala Arg Trp Ser Glu Leu Ser Asp Pro Leu Val Leu Val Met 
                85                  90                  95      


Thr Gly Ala Tyr Pro Lys Pro Thr Leu Ser Ala Gln Pro Ser Pro Val 
            100                 105                 110         


Val Thr Ser Gly Gly Arg Val Thr Leu Gln Cys Glu Ser Gln Val Ala 
        115                 120                 125             


Phe Gly Gly Phe Ile Leu Cys Lys Glu Gly Glu Glu Glu His Pro Gln 
    130                 135                 140                 


Cys Leu Asn Ser Gln Pro His Ala Arg Gly Ser Ser Arg Ala Ile Phe 
145                 150                 155                 160 


Ser Val Gly Pro Val Ser Pro Asn Arg Arg Trp Ser His Arg Cys Tyr 
                165                 170                 175     


Gly Tyr Asp Leu Asn Ser Pro Tyr Val Trp Ser Ser Pro Ser Asp Leu 
            180                 185                 190         


Leu Glu Leu Leu Val Pro Gly Val Ser Lys Lys Pro Ser Leu Ser Val 
        195                 200                 205             


Gln Pro Gly Pro Val Val Ala Pro Gly Glu Ser Leu Thr Leu Gln Cys 
    210                 215                 220                 


Val Ser Asp Val Gly Tyr Asp Arg Phe Val Leu Tyr Lys Glu Gly Glu 
225                 230                 235                 240 


Arg Asp Leu Arg Gln Leu Pro Gly Arg Gln Pro Gln Ala Gly Leu Ser 
                245                 250                 255     


Gln Ala Asn Phe Thr Leu Gly Pro Val Ser Arg Ser Tyr Gly Gly Gln 
            260                 265                 270         


Tyr Arg Cys Tyr Gly Ala His Asn Leu Ser Ser Glu Cys Ser Ala Pro 
        275                 280                 285             


Ser Asp Pro Leu Asp Ile Leu Ile Thr Gly Gln Ile Arg Gly Thr Pro 
    290                 295                 300                 


Phe Ile Ser Val Gln Pro Gly Pro Thr Val Ala Ser Gly Glu Asn Val 
305                 310                 315                 320 


Thr Leu Leu Cys Gln Ser Trp Arg Gln Phe His Thr Phe Leu Leu Thr 
                325                 330                 335     


Lys Ala Gly Ala Ala Asp Ala Pro Leu Arg Leu Arg Ser Ile His Glu 
            340                 345                 350         


Tyr Pro Lys Tyr Gln Ala Glu Phe Pro Met Ser Pro Val Thr Ser Ala 
        355                 360                 365             


His Ala Gly Thr Tyr Arg Cys Tyr Gly Ser Leu Asn Ser Asp Pro Tyr 
    370                 375                 380                 


Leu Leu Ser His Pro Ser Glu Pro Leu Glu Leu Val Val Ser Gly Gly 
385                 390                 395                 400 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Pro Lys 
                405                 410                 415     


Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu 
            420                 425                 430         


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
        435                 440                 445             


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
    450                 455                 460                 


Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val 
465                 470                 475                 480 


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 
                485                 490                 495     


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
            500                 505                 510         


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 
        515                 520                 525             


Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
    530                 535                 540                 


Gln Val Cys Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln 
545                 550                 555                 560 


Val Ser Leu Ser Cys Ala Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
                565                 570                 575     


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
            580                 585                 590         


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Val Ser Lys Leu 
        595                 600                 605             


Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 
    610                 615                 620                 


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
625                 630                 635                 640 


Leu Ser Pro Gly Lys 
                645 


<210>  12
<211>  1935
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NA of #11

<400>  12
cagaccggca caatcccaaa gcccaccctg tgggccgagc ctgattccgt gatcacccag       60

ggctctccag tgacactgtc ctgccagggc tctctggagg cccaggagta ccggctgtat      120

agagagaaga agtctgccag ctggatcacc cggatcagac ctgagctggt gaagaacggc      180

cagtttcaca tcccaagcat cacctgggag cacacaggcc ggtacggatg ccagtactat      240

tcccgggcca gatggagcga gctgtccgac cctctggtgc tggtcatgac cggcgcctat      300

cctaagccaa cactgagcgc ccagccatcc cctgtggtga ccagcggcgg cagagtgaca      360

ctgcagtgtg agtcccaggt ggccttcggc ggctttatcc tgtgcaagga gggcgaggag      420

gagcacccac agtgtctgaa cagccagcca cacgcccggg gcagctccag agccatcttc      480

tccgtgggac ccgtgagccc aaaccggaga tggagccacc ggtgctacgg ctatgacctg      540

aatagccctt acgtgtggtc tagcccatcc gatctgctgg agctgctggt gcccggcgtg      600

tccaagaagc cttccctgtc tgtgcagcca ggaccagtgg tggcaccagg agagtctctg      660

accctgcagt gcgtgagcga cgtgggctac gatcggttcg tgctgtataa ggagggagag      720

agggatctga ggcagctgcc aggcagacag ccacaggccg gcctgagcca ggccaacttt      780

acactgggcc cagtgagcag gtcctatggc ggacagtaca ggtgctatgg agcacacaat      840

ctgtcctctg agtgttctgc ccccagcgac cccctggaca tcctgatcac cggccagatc      900

aggggcacac ccttcatctc cgtgcagcct ggaccaaccg tggcctctgg cgagaacgtg      960

acactgctgt gccagtcttg gcgccagttc cacacctttc tgctgacaaa ggcaggagca     1020

gcagacgcac cactgaggct gcgcagcatc cacgagtacc ccaagtatca ggccgagttt     1080

ccaatgtctc cagtgaccag cgcccacgca ggcacataca ggtgttatgg cagcctgaac     1140

agcgacccct acctgctgag ccacccttcc gagccactgg agctggtggt gagcggagga     1200

ggaggctccg gaggaggagg ctctggcggc ggcggcagcg agcctaagag ctccgacaag     1260

acccacacat gcccaccttg tccagcacct gagctgctgg gaggaccatc cgtgttcctg     1320

tttccaccca agcctaagga taccctgatg atctctcgca cccctgaggt gacatgcgtg     1380

gtggtggacg tgagccacga ggaccccgag gtgaagttta actggtacgt ggacggcgtg     1440

gaggtgcaca atgccaagac aaagccccgg gaggagcagt acaacagcac ctatagagtg     1500

gtgtccgtgc tgacagtgct gcaccaggat tggctgaacg gcaaggagta caagtgtaag     1560

gtgtccaata aggccctgcc agcccccatc gagaagacca tctctaaggc aaagggacag     1620

cccagggagc ctcaggtgtg caccctgcct ccaagccgcg acgagctgac aaagaaccag     1680

gtgtctctga gctgtgccgt gaagggcttc tacccatctg acatcgccgt ggagtgggag     1740

agcaatggcc agcccgagaa caattataag accacacccc ctgtgctgga ctctgatggc     1800

agcttctttc tggtgtccaa gctgaccgtg gataagtcta ggtggcagca gggcaacgtg     1860

ttttcctgtt ctgtgatgca cgaggccctg cacaatcact acacacagaa gagcctgtcc     1920

ctgtctcccg gcaag                                                      1935


<210>  13
<211>  351
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TIGIT Fc (IgG4 knob) 351 AA first monomer of DSP205V1

<400>  13

Met Thr Gly Thr Ile Glu Thr Thr Gly Asn Ile Ser Ala Glu Lys Gly 
1               5                   10                  15      


Gly Ser Ile Ala Leu Gln Cys His Leu Ser Ser Thr Thr Ala Gln Val 
            20                  25                  30          


Thr Gln Val Asn Trp Glu Gln Gln Asp Gln Leu Leu Ala Ile Ser Asn 
        35                  40                  45              


Ala Asp Leu Gly Trp His Ile Ser Pro Ser Phe Lys Asp Arg Val Ala 
    50                  55                  60                  


Pro Gly Pro Gly Leu Gly Leu Thr Leu Gln Ser Leu Thr Val Asn Asp 
65                  70                  75                  80  


Thr Gly Glu Tyr Phe Cys Ile Tyr His Thr Tyr Pro Asp Gly Thr Tyr 
                85                  90                  95      


Thr Gly Arg Ile Phe Leu Glu Val Leu Glu Ser Ser Val Ala Glu His 
            100                 105                 110         


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ser Lys Tyr Gly Pro 
        115                 120                 125             


Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Glu Gly Gly Pro Ser Val 
    130                 135                 140                 


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
145                 150                 155                 160 


Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu 
                165                 170                 175     


Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
            180                 185                 190         


Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 
        195                 200                 205             


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
    210                 215                 220                 


Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile 
225                 230                 235                 240 


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Cys Thr Leu Pro 
                245                 250                 255     


Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Trp Cys Leu 
            260                 265                 270         


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
        275                 280                 285             


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
    290                 295                 300                 


Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg 
305                 310                 315                 320 


Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
                325                 330                 335     


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys 
            340                 345                 350     


<210>  14
<211>  1053
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA of#13

<400>  14
atgaccggca caatcgagac aacaggcaac atctctgccg agaagggagg cagcatcgcc       60

ctgcagtgcc acctgagcag caccacagcc caggtgaccc aggtgaactg ggagcagcag      120

gaccagctgc tggccatctc caatgccgat ctgggctggc acatcagccc ctcctttaag      180

gatagggtgg cacctggacc aggcctgggc ctgaccctgc agagcctgac cgtgaatgac      240

acaggcgagt acttctgtat ctaccacaca tatcctgatg gcacctatac aggcagaatc      300

tttctggagg tgctggagtc tagcgtggcc gagcacggag gaggaggctc cggaggagga      360

ggctctgaga gcaagtacgg accaccttgc ccaccatgtc cagcacctga gttcgaggga      420

ggacctagcg tgttcctgtt tcctccaaag ccaaaggaca ccctgatgat cagcaggacc      480

cctgaggtga catgcgtggt ggtggacgtg tcccaggagg accccgaggt gcagttcaac      540

tggtatgtgg atggcgtgga ggtgcacaat gccaagacaa agcccaggga ggagcagttt      600

aactccacct accgcgtggt gtctgtgctg acagtgctgc accaggactg gctgaacggc      660

aaggagtata agtgtaaggt gtctaataag ggcctgccct cctctatcga gaaaaccatc      720

agcaaggcca agggccagcc aagagagcca caggtgtgca ccctgccacc ttcccaggag      780

gagatgacaa agaaccaggt gtctctgtgg tgtctggtga agggcttcta cccatctgac      840

atcgccgtgg agtgggagag caatggccag cccgagaaca attacaagac cacaccaccc      900

gtgctggaca gcgatggctc cttctttctg tatagccggc tgaccgtgga taagtccaga      960

tggcaggagg gcaacgtgtt ttcctgctct gtgatgcacg aggccctgca caatcactat     1020

acacagaaga gcctgtccct gtctctgggc aag                                  1053


<210>  15
<211>  642
<212>  PRT
<213>  Artificial sequence

<220>
<223>  LILRB Fc (IgG4 hole) 642 AA second monomer of DSP205V1, DSP216V1,
       DSP412V1

<400>  15

Gln Thr Gly Thr Ile Pro Lys Pro Thr Leu Trp Ala Glu Pro Asp Ser 
1               5                   10                  15      


Val Ile Thr Gln Gly Ser Pro Val Thr Leu Ser Cys Gln Gly Ser Leu 
            20                  25                  30          


Glu Ala Gln Glu Tyr Arg Leu Tyr Arg Glu Lys Lys Ser Ala Ser Trp 
        35                  40                  45              


Ile Thr Arg Ile Arg Pro Glu Leu Val Lys Asn Gly Gln Phe His Ile 
    50                  55                  60                  


Pro Ser Ile Thr Trp Glu His Thr Gly Arg Tyr Gly Cys Gln Tyr Tyr 
65                  70                  75                  80  


Ser Arg Ala Arg Trp Ser Glu Leu Ser Asp Pro Leu Val Leu Val Met 
                85                  90                  95      


Thr Gly Ala Tyr Pro Lys Pro Thr Leu Ser Ala Gln Pro Ser Pro Val 
            100                 105                 110         


Val Thr Ser Gly Gly Arg Val Thr Leu Gln Cys Glu Ser Gln Val Ala 
        115                 120                 125             


Phe Gly Gly Phe Ile Leu Cys Lys Glu Gly Glu Glu Glu His Pro Gln 
    130                 135                 140                 


Cys Leu Asn Ser Gln Pro His Ala Arg Gly Ser Ser Arg Ala Ile Phe 
145                 150                 155                 160 


Ser Val Gly Pro Val Ser Pro Asn Arg Arg Trp Ser His Arg Cys Tyr 
                165                 170                 175     


Gly Tyr Asp Leu Asn Ser Pro Tyr Val Trp Ser Ser Pro Ser Asp Leu 
            180                 185                 190         


Leu Glu Leu Leu Val Pro Gly Val Ser Lys Lys Pro Ser Leu Ser Val 
        195                 200                 205             


Gln Pro Gly Pro Val Val Ala Pro Gly Glu Ser Leu Thr Leu Gln Cys 
    210                 215                 220                 


Val Ser Asp Val Gly Tyr Asp Arg Phe Val Leu Tyr Lys Glu Gly Glu 
225                 230                 235                 240 


Arg Asp Leu Arg Gln Leu Pro Gly Arg Gln Pro Gln Ala Gly Leu Ser 
                245                 250                 255     


Gln Ala Asn Phe Thr Leu Gly Pro Val Ser Arg Ser Tyr Gly Gly Gln 
            260                 265                 270         


Tyr Arg Cys Tyr Gly Ala His Asn Leu Ser Ser Glu Cys Ser Ala Pro 
        275                 280                 285             


Ser Asp Pro Leu Asp Ile Leu Ile Thr Gly Gln Ile Arg Gly Thr Pro 
    290                 295                 300                 


Phe Ile Ser Val Gln Pro Gly Pro Thr Val Ala Ser Gly Glu Asn Val 
305                 310                 315                 320 


Thr Leu Leu Cys Gln Ser Trp Arg Gln Phe His Thr Phe Leu Leu Thr 
                325                 330                 335     


Lys Ala Gly Ala Ala Asp Ala Pro Leu Arg Leu Arg Ser Ile His Glu 
            340                 345                 350         


Tyr Pro Lys Tyr Gln Ala Glu Phe Pro Met Ser Pro Val Thr Ser Ala 
        355                 360                 365             


His Ala Gly Thr Tyr Arg Cys Tyr Gly Ser Leu Asn Ser Asp Pro Tyr 
    370                 375                 380                 


Leu Leu Ser His Pro Ser Glu Pro Leu Glu Leu Val Val Ser Gly Gly 
385                 390                 395                 400 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ser Lys 
                405                 410                 415     


Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Glu Gly Gly 
            420                 425                 430         


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
        435                 440                 445             


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu 
    450                 455                 460                 


Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
465                 470                 475                 480 


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg 
                485                 490                 495     


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
            500                 505                 510         


Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu 
        515                 520                 525             


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
    530                 535                 540                 


Thr Leu Pro Pro Ser Gln Cys Glu Met Thr Lys Asn Gln Val Ser Leu 
545                 550                 555                 560 


Ser Cys Ala Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
                565                 570                 575     


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
            580                 585                 590         


Leu Asp Ser Asp Gly Ser Phe Phe Leu Val Ser Arg Leu Thr Val Asp 
        595                 600                 605             


Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His 
    610                 615                 620                 


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu 
625                 630                 635                 640 


Gly Lys 
        


<210>  16
<211>  1926
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA of#15

<400>  16
cagaccggca caatccctaa gccaaccctg tgggccgagc ctgatagcgt gatcacccag       60

ggctccccag tgacactgag ctgccagggc tccctggagg cacaggagta ccggctgtat      120

agagagaaga agtctgccag ctggatcacc cggatcagac ctgagctggt gaagaacggc      180

cagttccaca tcccatctat cacctgggag cacacaggcc ggtacggatg ccagtactat      240

agccgggcca gatggtctga gctgagcgac cccctggtgc tggtcatgac cggagcctat      300

cccaagccta cactgtctgc ccagccaagc ccagtggtga cctctggcgg cagagtgaca      360

ctgcagtgtg agagccaggt ggccttcggc ggctttatcc tgtgcaagga gggcgaggag      420

gagcacccac agtgtctgaa tagccagcca cacgccaggg gcagctcccg cgccatcttc      480

agcgtgggac ccgtgagccc aaaccggaga tggtcccacc gctgctacgg ctatgacctg      540

aacagccctt acgtgtggtc tagcccaagc gatctgctgg agctgctggt gcccggcgtg      600

agcaagaagc cttccctgtc tgtgcagcct ggaccagtgg tggcacctgg agagtccctg      660

accctgcagt gcgtgagcga cgtgggctac gatcggtttg tgctgtataa ggagggagag      720

agggatctga ggcagctgcc aggcagacag ccacaggccg gcctgtccca ggccaacttc      780

accctgggcc cagtgagccg gtcctatggc ggccagtaca gatgctatgg cgcccacaat      840

ctgtcctctg agtgttccgc cccaagcgac cccctggaca tcctgatcac cggccagatc      900

aggggcacac cctttatcag cgtgcagcca ggacctaccg tggcctccgg cgagaacgtg      960

acactgctgt gccagagctg gcgccagttc cacacctttc tgctgacaaa ggcaggagca     1020

gcagacgcac cactgaggct gcgctccatc cacgagtacc ccaagtatca ggccgagttc     1080

ccaatgtccc cagtgacctc tgcccacgca ggcacataca ggtgttatgg cagcctgaac     1140

agcgacccct acctgctgtc tcaccctagc gagccactgg agctggtggt gtctggagga     1200

ggaggcagcg gcggaggagg ctccggaggc ggcggctctg agagcaagta tggaccacct     1260

tgcccaccat gtccagcacc agagttcgag ggaggaccaa gcgtgttcct gtttcctcca     1320

aagcctaagg acaccctgat gatctcccgc acccctgagg tgacatgcgt ggtggtggac     1380

gtgtctcagg aggaccccga ggtgcagttt aactggtacg tggatggcgt ggaggtgcac     1440

aatgccaaga ccaagccccg ggaggagcag ttcaactcta cctacagagt ggtgagcgtg     1500

ctgacagtgc tgcaccagga ctggctgaac ggcaaggagt ataagtgtaa ggtgagcaat     1560

aagggcctgc ctagctccat cgagaaaacc atcagcaagg caaagggaca gcccagggag     1620

cctcaggtgt ataccctgcc cccttcccag tgcgagatga caaagaacca ggtgtccctg     1680

tcttgtgccg tgaagggctt ttacccatcc gacatcgccg tggagtggga gtctaatggc     1740

cagcccgaga acaattataa gaccacacca cccgtgctgg actccgatgg ctctttcttt     1800

ctggtgagca ggctgaccgt ggataagtcc cgctggcagg agggcaacgt gttcagctgc     1860

tccgtgatgc acgaggccct gcacaatcac tacacacaga agtctctgag cctgtccctg     1920

ggcaag                                                                1926


<210>  17
<211>  585
<212>  PRT
<213>  Artificial sequence

<220>
<223>  SIRP alpha Fc (IgG1 knob as IgG4) 585AA first monomer of DSP216V2

<400>  17

Glu Glu Glu Leu Gln Val Ile Gln Pro Asp Lys Ser Val Leu Val Ala 
1               5                   10                  15      


Ala Gly Glu Thr Ala Thr Leu Arg Cys Thr Ala Thr Ser Leu Ile Pro 
            20                  25                  30          


Val Gly Pro Ile Gln Trp Phe Arg Gly Ala Gly Pro Gly Arg Glu Leu 
        35                  40                  45              


Ile Tyr Asn Gln Lys Glu Gly His Phe Pro Arg Val Thr Thr Val Ser 
    50                  55                  60                  


Asp Leu Thr Lys Arg Asn Asn Met Asp Phe Ser Ile Arg Ile Gly Asn 
65                  70                  75                  80  


Ile Thr Pro Ala Asp Ala Gly Thr Tyr Tyr Cys Val Lys Phe Arg Lys 
                85                  90                  95      


Gly Ser Pro Asp Asp Val Glu Phe Lys Ser Gly Ala Gly Thr Glu Leu 
            100                 105                 110         


Ser Val Arg Ala Lys Pro Ser Ala Pro Val Val Ser Gly Pro Ala Ala 
        115                 120                 125             


Arg Ala Thr Pro Gln His Thr Val Ser Phe Thr Cys Glu Ser His Gly 
    130                 135                 140                 


Phe Ser Pro Arg Asp Ile Thr Leu Lys Trp Phe Lys Asn Gly Asn Glu 
145                 150                 155                 160 


Leu Ser Asp Phe Gln Thr Asn Val Asp Pro Val Gly Glu Ser Val Ser 
                165                 170                 175     


Tyr Ser Ile His Ser Thr Ala Lys Val Val Leu Thr Arg Glu Asp Val 
            180                 185                 190         


His Ser Gln Val Ile Cys Glu Val Ala His Val Thr Leu Gln Gly Asp 
        195                 200                 205             


Pro Leu Arg Gly Thr Ala Asn Leu Ser Glu Thr Ile Arg Val Pro Pro 
    210                 215                 220                 


Thr Leu Glu Val Thr Gln Gln Pro Val Arg Ala Glu Asn Gln Val Asn 
225                 230                 235                 240 


Val Thr Cys Gln Val Arg Lys Phe Tyr Pro Gln Arg Leu Gln Leu Thr 
                245                 250                 255     


Trp Leu Glu Asn Gly Asn Val Ser Arg Thr Glu Thr Ala Ser Thr Val 
            260                 265                 270         


Thr Glu Asn Lys Asp Gly Thr Tyr Asn Trp Met Ser Trp Leu Leu Val 
        275                 280                 285             


Asn Val Ser Ala His Arg Asp Asp Val Lys Leu Thr Cys Gln Val Glu 
    290                 295                 300                 


His Asp Gly Gln Pro Ala Val Ser Lys Ser His Asp Leu Lys Val Ser 
305                 310                 315                 320 


Ala His Pro Lys Glu Gln Gly Ser Asn Thr Ala Ala Glu Asn Thr Gly 
                325                 330                 335     


Ser Asn Glu Arg Asn Ile Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly 
            340                 345                 350         


Ser Glu Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
        355                 360                 365             


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
    370                 375                 380                 


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
385                 390                 395                 400 


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
                405                 410                 415     


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
            420                 425                 430         


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
        435                 440                 445             


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
    450                 455                 460                 


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
465                 470                 475                 480 


Pro Arg Glu Pro Gln Val Cys Thr Leu Pro Pro Ser Arg Asp Glu Leu 
                485                 490                 495     


Thr Lys Asn Gln Val Ser Leu Trp Cys Leu Val Lys Gly Phe Tyr Pro 
            500                 505                 510         


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
        515                 520                 525             


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
    530                 535                 540                 


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
545                 550                 555                 560 


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
                565                 570                 575     


Lys Ser Leu Ser Leu Ser Pro Gly Lys 
            580                 585 


<210>  18
<211>  1755
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA of#17

<400>  18
gaggaggagc tgcaggtcat ccagcccgat aagtctgtgc tggtggcagc aggagagacc       60

gccacactga ggtgcaccgc cacaagcctg atcccagtgg gaccaatcca gtggtttagg      120

ggagcaggcc ctggcagaga gctgatctac aaccagaagg agggccactt cccaagagtg      180

accacagtga gcgacctgac caagcggaac aatatggatt tttccatcag aatcggcaat      240

atcacacctg ccgacgccgg cacctactat tgcgtgaagt tcaggaaggg ctccccagac      300

gatgtggagt ttaagagcgg agcaggcacc gagctgtccg tgcgggcaaa gccttccgcc      360

ccagtggtgt ctggaccagc agccagagcc accccacagc acacagtgtc cttcacctgt      420

gagtctcacg gctttagccc ccgggacatc accctgaagt ggttcaagaa cggcaatgag      480

ctgtctgact ttcagaccaa cgtggacccc gtgggcgagt ctgtgagcta ttccatccac      540

tctacagcca aggtggtgct gacccgcgag gacgtgcaca gccaggtcat ctgcgaggtg      600

gcacacgtga ccctgcaggg cgatcctctg aggggcacag ccaatctgag cgagaccatc      660

agagtgcccc ctacactgga ggtgacccag cagcccgtgc gcgcagagaa ccaagtgaat      720

gtgacatgtc aggtgaggaa gttctaccct cagcgcctgc agctgacctg gctggagaac      780

ggcaacgtga gccggaccga gacagccagc accgtgacag agaacaagga cggcacatat      840

aattggatgt cttggctgct ggtgaacgtg agcgcccaca gggacgatgt gaagctgacc      900

tgccaggtgg agcacgacgg acagccagcc gtgtctaaga gccacgatct gaaggtgagc      960

gcccacccta aggagcaggg ctccaacaca gccgccgaga ataccggcag caacgagcgg     1020

aatatctacg gaggaggagg cagcggagga ggaggctccg agcctaagag ctccgacaag     1080

acccacacat gcccaccatg tcctgcacca gagctgctgg gaggaccttc cgtgttcctg     1140

tttcctccaa agccaaagga tacactgatg atctccagaa caccagaggt gacctgcgtg     1200

gtggtggacg tgtctcacga ggaccccgag gtgaagttta actggtacgt ggacggcgtg     1260

gaggtgcaca atgccaagac caagccaagg gaggagcagt acaactccac atatcgcgtg     1320

gtgtctgtgc tgaccgtgct gcaccaggat tggctgaacg gcaaggagta taagtgtaag     1380

gtgagcaata aggccctgcc cgcccctatc gagaagacca tctccaaggc aaagggacag     1440

cccagggagc ctcaggtgtg cacactgccc ccttcccgcg acgagctgac caagaaccag     1500

gtgtctctgt ggtgtctggt gaagggcttc tacccatctg acatcgccgt ggagtgggag     1560

agcaatggcc agcccgagaa caattacaag accacaccac ccgtgctgga cagcgatggc     1620

tccttctttc tgtattccaa gctgacagtg gacaagtctc ggtggcagca gggcaacgtg     1680

ttttcctgtt ctgtgatgca cgaggccctg cacaatcact atacccagaa gagcctgtcc     1740

ctgtctcccg gcaag                                                      1755


<210>  19
<211>  645
<212>  PRT
<213>  Artificial sequence

<220>
<223>  LILRB2 FC (IgG1 hole as IgG4) 645 AA second monomer of DSP216V2

<400>  19

Gln Thr Gly Thr Ile Pro Lys Pro Thr Leu Trp Ala Glu Pro Asp Ser 
1               5                   10                  15      


Val Ile Thr Gln Gly Ser Pro Val Thr Leu Ser Cys Gln Gly Ser Leu 
            20                  25                  30          


Glu Ala Gln Glu Tyr Arg Leu Tyr Arg Glu Lys Lys Ser Ala Ser Trp 
        35                  40                  45              


Ile Thr Arg Ile Arg Pro Glu Leu Val Lys Asn Gly Gln Phe His Ile 
    50                  55                  60                  


Pro Ser Ile Thr Trp Glu His Thr Gly Arg Tyr Gly Cys Gln Tyr Tyr 
65                  70                  75                  80  


Ser Arg Ala Arg Trp Ser Glu Leu Ser Asp Pro Leu Val Leu Val Met 
                85                  90                  95      


Thr Gly Ala Tyr Pro Lys Pro Thr Leu Ser Ala Gln Pro Ser Pro Val 
            100                 105                 110         


Val Thr Ser Gly Gly Arg Val Thr Leu Gln Cys Glu Ser Gln Val Ala 
        115                 120                 125             


Phe Gly Gly Phe Ile Leu Cys Lys Glu Gly Glu Glu Glu His Pro Gln 
    130                 135                 140                 


Cys Leu Asn Ser Gln Pro His Ala Arg Gly Ser Ser Arg Ala Ile Phe 
145                 150                 155                 160 


Ser Val Gly Pro Val Ser Pro Asn Arg Arg Trp Ser His Arg Cys Tyr 
                165                 170                 175     


Gly Tyr Asp Leu Asn Ser Pro Tyr Val Trp Ser Ser Pro Ser Asp Leu 
            180                 185                 190         


Leu Glu Leu Leu Val Pro Gly Val Ser Lys Lys Pro Ser Leu Ser Val 
        195                 200                 205             


Gln Pro Gly Pro Val Val Ala Pro Gly Glu Ser Leu Thr Leu Gln Cys 
    210                 215                 220                 


Val Ser Asp Val Gly Tyr Asp Arg Phe Val Leu Tyr Lys Glu Gly Glu 
225                 230                 235                 240 


Arg Asp Leu Arg Gln Leu Pro Gly Arg Gln Pro Gln Ala Gly Leu Ser 
                245                 250                 255     


Gln Ala Asn Phe Thr Leu Gly Pro Val Ser Arg Ser Tyr Gly Gly Gln 
            260                 265                 270         


Tyr Arg Cys Tyr Gly Ala His Asn Leu Ser Ser Glu Cys Ser Ala Pro 
        275                 280                 285             


Ser Asp Pro Leu Asp Ile Leu Ile Thr Gly Gln Ile Arg Gly Thr Pro 
    290                 295                 300                 


Phe Ile Ser Val Gln Pro Gly Pro Thr Val Ala Ser Gly Glu Asn Val 
305                 310                 315                 320 


Thr Leu Leu Cys Gln Ser Trp Arg Gln Phe His Thr Phe Leu Leu Thr 
                325                 330                 335     


Lys Ala Gly Ala Ala Asp Ala Pro Leu Arg Leu Arg Ser Ile His Glu 
            340                 345                 350         


Tyr Pro Lys Tyr Gln Ala Glu Phe Pro Met Ser Pro Val Thr Ser Ala 
        355                 360                 365             


His Ala Gly Thr Tyr Arg Cys Tyr Gly Ser Leu Asn Ser Asp Pro Tyr 
    370                 375                 380                 


Leu Leu Ser His Pro Ser Glu Pro Leu Glu Leu Val Val Ser Gly Gly 
385                 390                 395                 400 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Pro Lys 
                405                 410                 415     


Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu 
            420                 425                 430         


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
        435                 440                 445             


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
    450                 455                 460                 


Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val 
465                 470                 475                 480 


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 
                485                 490                 495     


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
            500                 505                 510         


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 
        515                 520                 525             


Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
    530                 535                 540                 


Gln Val Tyr Thr Leu Pro Pro Ser Arg Cys Glu Leu Thr Lys Asn Gln 
545                 550                 555                 560 


Val Ser Leu Ser Cys Ala Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
                565                 570                 575     


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
            580                 585                 590         


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Val Ser Lys Leu 
        595                 600                 605             


Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 
    610                 615                 620                 


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
625                 630                 635                 640 


Leu Ser Pro Gly Lys 
                645 


<210>  20
<211>  1935
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA of#19

<400>  20
cagaccggca caatcccaaa gcccaccctg tgggccgagc ctgattccgt gatcacccag       60

ggctctccag tgacactgtc ctgccagggc tctctggagg cccaggagta ccggctgtat      120

agagagaaga agtctgccag ctggatcacc cggatcagac ctgagctggt gaagaacggc      180

cagtttcaca tcccaagcat cacctgggag cacacaggcc ggtacggatg ccagtactat      240

tcccgggcca gatggagcga gctgtccgac cctctggtgc tggtcatgac cggcgcctat      300

cctaagccaa cactgagcgc ccagccatcc cctgtggtga ccagcggcgg cagagtgaca      360

ctgcagtgtg agtcccaggt ggccttcggc ggctttatcc tgtgcaagga gggcgaggag      420

gagcacccac agtgtctgaa cagccagcca cacgcccggg gcagctccag agccatcttc      480

tccgtgggac ccgtgagccc aaaccggaga tggagccacc ggtgctacgg ctatgacctg      540

aatagccctt acgtgtggtc tagcccatcc gatctgctgg agctgctggt gcccggcgtg      600

tccaagaagc cttccctgtc tgtgcagcca ggaccagtgg tggcaccagg agagtctctg      660

accctgcagt gcgtgagcga cgtgggctac gatcggttcg tgctgtataa ggagggagag      720

agggatctga ggcagctgcc aggcagacag ccacaggccg gcctgagcca ggccaacttt      780

acactgggcc cagtgagcag gtcctatggc ggacagtaca ggtgctatgg agcacacaat      840

ctgtcctctg agtgttctgc ccccagcgac cccctggaca tcctgatcac cggccagatc      900

aggggcacac ccttcatctc cgtgcagcct ggaccaaccg tggcctctgg cgagaacgtg      960

acactgctgt gccagtcttg gcgccagttc cacacctttc tgctgacaaa ggcaggagca     1020

gcagacgcac cactgaggct gcgcagcatc cacgagtacc ccaagtatca ggccgagttt     1080

ccaatgtctc cagtgaccag cgcccacgca ggcacataca ggtgttatgg cagcctgaac     1140

agcgacccct acctgctgag ccacccttcc gagccactgg agctggtggt gagcggagga     1200

ggaggctccg gaggaggagg ctctggcggc ggcggcagcg agcctaagag ctccgacaag     1260

acccacacat gcccaccttg tccagcacct gagctgctgg gaggaccatc cgtgttcctg     1320

tttccaccca agcctaagga taccctgatg atctctcgca cccctgaggt gacatgcgtg     1380

gtggtggacg tgagccacga ggaccccgag gtgaagttta actggtacgt ggacggcgtg     1440

gaggtgcaca atgccaagac aaagccccgg gaggagcagt acaacagcac ctatagagtg     1500

gtgtccgtgc tgacagtgct gcaccaggat tggctgaacg gcaaggagta caagtgtaag     1560

gtgtccaata aggccctgcc agcccccatc gagaagacca tctctaaggc aaagggacag     1620

cccagggagc ctcaggtgta taccctgcct ccaagccgct gcgagctgac aaagaaccag     1680

gtgtctctga gctgtgccgt gaagggcttc tacccatctg acatcgccgt ggagtgggag     1740

agcaatggcc agcccgagaa caattataag accacacccc ctgtgctgga ctctgatggc     1800

agcttctttc tggtgtccaa gctgaccgtg gataagtcta ggtggcagca gggcaacgtg     1860

ttttcctgtt ctgtgatgca cgaggccctg cacaatcact acacacagaa gagcctgtcc     1920

ctgtctcccg gcaag                                                      1935


<210>  21
<211>  645
<212>  PRT
<213>  Artificial sequence

<220>
<223>  LILRB2 Fc (IgG1 knob)  645 AA first monomer of DSP220

<400>  21

Gln Thr Gly Thr Ile Pro Lys Pro Thr Leu Trp Ala Glu Pro Asp Ser 
1               5                   10                  15      


Val Ile Thr Gln Gly Ser Pro Val Thr Leu Ser Cys Gln Gly Ser Leu 
            20                  25                  30          


Glu Ala Gln Glu Tyr Arg Leu Tyr Arg Glu Lys Lys Ser Ala Ser Trp 
        35                  40                  45              


Ile Thr Arg Ile Arg Pro Glu Leu Val Lys Asn Gly Gln Phe His Ile 
    50                  55                  60                  


Pro Ser Ile Thr Trp Glu His Thr Gly Arg Tyr Gly Cys Gln Tyr Tyr 
65                  70                  75                  80  


Ser Arg Ala Arg Trp Ser Glu Leu Ser Asp Pro Leu Val Leu Val Met 
                85                  90                  95      


Thr Gly Ala Tyr Pro Lys Pro Thr Leu Ser Ala Gln Pro Ser Pro Val 
            100                 105                 110         


Val Thr Ser Gly Gly Arg Val Thr Leu Gln Cys Glu Ser Gln Val Ala 
        115                 120                 125             


Phe Gly Gly Phe Ile Leu Cys Lys Glu Gly Glu Glu Glu His Pro Gln 
    130                 135                 140                 


Cys Leu Asn Ser Gln Pro His Ala Arg Gly Ser Ser Arg Ala Ile Phe 
145                 150                 155                 160 


Ser Val Gly Pro Val Ser Pro Asn Arg Arg Trp Ser His Arg Cys Tyr 
                165                 170                 175     


Gly Tyr Asp Leu Asn Ser Pro Tyr Val Trp Ser Ser Pro Ser Asp Leu 
            180                 185                 190         


Leu Glu Leu Leu Val Pro Gly Val Ser Lys Lys Pro Ser Leu Ser Val 
        195                 200                 205             


Gln Pro Gly Pro Val Val Ala Pro Gly Glu Ser Leu Thr Leu Gln Cys 
    210                 215                 220                 


Val Ser Asp Val Gly Tyr Asp Arg Phe Val Leu Tyr Lys Glu Gly Glu 
225                 230                 235                 240 


Arg Asp Leu Arg Gln Leu Pro Gly Arg Gln Pro Gln Ala Gly Leu Ser 
                245                 250                 255     


Gln Ala Asn Phe Thr Leu Gly Pro Val Ser Arg Ser Tyr Gly Gly Gln 
            260                 265                 270         


Tyr Arg Cys Tyr Gly Ala His Asn Leu Ser Ser Glu Cys Ser Ala Pro 
        275                 280                 285             


Ser Asp Pro Leu Asp Ile Leu Ile Thr Gly Gln Ile Arg Gly Thr Pro 
    290                 295                 300                 


Phe Ile Ser Val Gln Pro Gly Pro Thr Val Ala Ser Gly Glu Asn Val 
305                 310                 315                 320 


Thr Leu Leu Cys Gln Ser Trp Arg Gln Phe His Thr Phe Leu Leu Thr 
                325                 330                 335     


Lys Ala Gly Ala Ala Asp Ala Pro Leu Arg Leu Arg Ser Ile His Glu 
            340                 345                 350         


Tyr Pro Lys Tyr Gln Ala Glu Phe Pro Met Ser Pro Val Thr Ser Ala 
        355                 360                 365             


His Ala Gly Thr Tyr Arg Cys Tyr Gly Ser Leu Asn Ser Asp Pro Tyr 
    370                 375                 380                 


Leu Leu Ser His Pro Ser Glu Pro Leu Glu Leu Val Val Ser Gly Gly 
385                 390                 395                 400 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Pro Lys 
                405                 410                 415     


Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu 
            420                 425                 430         


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
        435                 440                 445             


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
    450                 455                 460                 


Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val 
465                 470                 475                 480 


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 
                485                 490                 495     


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
            500                 505                 510         


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 
        515                 520                 525             


Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
    530                 535                 540                 


Gln Val Tyr Thr Leu Pro Pro Cys Arg Asp Glu Leu Thr Lys Asn Gln 
545                 550                 555                 560 


Val Ser Leu Trp Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
                565                 570                 575     


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
            580                 585                 590         


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu 
        595                 600                 605             


Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 
    610                 615                 620                 


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
625                 630                 635                 640 


Leu Ser Pro Gly Lys 
                645 


<210>  22
<211>  642
<212>  PRT
<213>  Artificial sequence

<220>
<223>  LILRB2 Fc IgG4 knob  642 AA first monomer of DSP220V1

<400>  22

Gln Thr Gly Thr Ile Pro Lys Pro Thr Leu Trp Ala Glu Pro Asp Ser 
1               5                   10                  15      


Val Ile Thr Gln Gly Ser Pro Val Thr Leu Ser Cys Gln Gly Ser Leu 
            20                  25                  30          


Glu Ala Gln Glu Tyr Arg Leu Tyr Arg Glu Lys Lys Ser Ala Ser Trp 
        35                  40                  45              


Ile Thr Arg Ile Arg Pro Glu Leu Val Lys Asn Gly Gln Phe His Ile 
    50                  55                  60                  


Pro Ser Ile Thr Trp Glu His Thr Gly Arg Tyr Gly Cys Gln Tyr Tyr 
65                  70                  75                  80  


Ser Arg Ala Arg Trp Ser Glu Leu Ser Asp Pro Leu Val Leu Val Met 
                85                  90                  95      


Thr Gly Ala Tyr Pro Lys Pro Thr Leu Ser Ala Gln Pro Ser Pro Val 
            100                 105                 110         


Val Thr Ser Gly Gly Arg Val Thr Leu Gln Cys Glu Ser Gln Val Ala 
        115                 120                 125             


Phe Gly Gly Phe Ile Leu Cys Lys Glu Gly Glu Glu Glu His Pro Gln 
    130                 135                 140                 


Cys Leu Asn Ser Gln Pro His Ala Arg Gly Ser Ser Arg Ala Ile Phe 
145                 150                 155                 160 


Ser Val Gly Pro Val Ser Pro Asn Arg Arg Trp Ser His Arg Cys Tyr 
                165                 170                 175     


Gly Tyr Asp Leu Asn Ser Pro Tyr Val Trp Ser Ser Pro Ser Asp Leu 
            180                 185                 190         


Leu Glu Leu Leu Val Pro Gly Val Ser Lys Lys Pro Ser Leu Ser Val 
        195                 200                 205             


Gln Pro Gly Pro Val Val Ala Pro Gly Glu Ser Leu Thr Leu Gln Cys 
    210                 215                 220                 


Val Ser Asp Val Gly Tyr Asp Arg Phe Val Leu Tyr Lys Glu Gly Glu 
225                 230                 235                 240 


Arg Asp Leu Arg Gln Leu Pro Gly Arg Gln Pro Gln Ala Gly Leu Ser 
                245                 250                 255     


Gln Ala Asn Phe Thr Leu Gly Pro Val Ser Arg Ser Tyr Gly Gly Gln 
            260                 265                 270         


Tyr Arg Cys Tyr Gly Ala His Asn Leu Ser Ser Glu Cys Ser Ala Pro 
        275                 280                 285             


Ser Asp Pro Leu Asp Ile Leu Ile Thr Gly Gln Ile Arg Gly Thr Pro 
    290                 295                 300                 


Phe Ile Ser Val Gln Pro Gly Pro Thr Val Ala Ser Gly Glu Asn Val 
305                 310                 315                 320 


Thr Leu Leu Cys Gln Ser Trp Arg Gln Phe His Thr Phe Leu Leu Thr 
                325                 330                 335     


Lys Ala Gly Ala Ala Asp Ala Pro Leu Arg Leu Arg Ser Ile His Glu 
            340                 345                 350         


Tyr Pro Lys Tyr Gln Ala Glu Phe Pro Met Ser Pro Val Thr Ser Ala 
        355                 360                 365             


His Ala Gly Thr Tyr Arg Cys Tyr Gly Ser Leu Asn Ser Asp Pro Tyr 
    370                 375                 380                 


Leu Leu Ser His Pro Ser Glu Pro Leu Glu Leu Val Val Ser Gly Gly 
385                 390                 395                 400 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ser Lys 
                405                 410                 415     


Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Glu Gly Gly 
            420                 425                 430         


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
        435                 440                 445             


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu 
    450                 455                 460                 


Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
465                 470                 475                 480 


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg 
                485                 490                 495     


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
            500                 505                 510         


Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu 
        515                 520                 525             


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Cys 
    530                 535                 540                 


Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu 
545                 550                 555                 560 


Trp Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
                565                 570                 575     


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
            580                 585                 590         


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp 
        595                 600                 605             


Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His 
    610                 615                 620                 


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu 
625                 630                 635                 640 


Gly Lys 
        


<210>  23
<211>  1926
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA of#22

<400>  23
cagaccggca caatccccaa gcctaccctg tgggccgagc cagatagcgt gatcacccag       60

ggctcccccg tgacactgtc ttgccagggc agcctggagg cacaggagta ccggctgtat      120

agagagaaga agagcgcctc ctggatcacc cggatcagac ccgagctggt gaagaacggc      180

cagtttcaca tcccttccat cacctgggag cacacaggcc ggtacggatg ccagtactat      240

tctcgggcca gatggagcga gctgtccgac cccctggtgc tggtcatgac cggcgcctat      300

ccaaagccca cactgtccgc ccagccttct ccagtggtga cctctggcgg cagagtgaca      360

ctgcagtgtg agagccaggt ggccttcggc ggctttatcc tgtgcaagga gggcgaggag      420

gagcaccccc agtgtctgaa tagccagcct cacgcccggg gcagctccag agccatcttc      480

tctgtgggcc ctgtgagccc aaaccggaga tggtcccaca ggtgctacgg ctatgacctg      540

aacagcccat acgtgtggtc tagcccctct gatctgctgg agctgctggt gcctggcgtg      600

agcaagaagc catctctgag cgtgcagcca ggccctgtgg tggcacctgg cgagtctctg      660

accctgcagt gcgtgagcga cgtgggctac gatcggttcg tgctgtataa ggagggagag      720

agggatctga ggcagctgcc aggcagacag cctcaggcag gactgtccca ggcaaacttt      780

acactgggcc ccgtgagccg gagctacggc ggacagtacc gctgctatgg agcacacaat      840

ctgtcctctg agtgttctgc ccccagcgac cccctggaca tcctgatcac cggccagatc      900

aggggcacac cattcatcag cgtgcagcca ggaccaaccg tggcctccgg cgagaacgtg      960

acactgctgt gccagagctg gcgccagttc cacacctttc tgctgacaaa ggcaggagca     1020

gcagacgcac ctctgaggct gcgctccatc cacgagtacc caaagtatca ggccgagttt     1080

ccaatgagcc ccgtgacctc cgcccacgca ggcacataca gatgctatgg cagcctgaac     1140

agcgacccct acctgctgag ccacccttcc gagccactgg agctggtggt gtccggcggc     1200

ggcggctctg gcggaggagg cagcggagga ggaggatccg agtctaagta cggaccacca     1260

tgccctccat gtcctgcacc agagttcgag ggaggaccat ccgtgttcct gtttccacct     1320

aagcctaagg acaccctgat gatctccaga acccccgagg tgacatgcgt ggtggtggac     1380

gtgtctcagg aggatcctga ggtgcagttc aattggtacg tggatggcgt ggaggtgcac     1440

aacgccaaga caaagccccg ggaggagcag tttaatagca cctacagagt ggtgtccgtg     1500

ctgacagtgc tgcaccagga ttggctgaat ggcaaggagt ataagtgtaa ggtgagcaac     1560

aagggcctgc ctagctccat cgagaagacc atctccaagg ccaagggcca gccaagagag     1620

ccacaggtgt gcaccctgcc accaagccag gaggagatga caaagaatca ggtgtccctg     1680

tggtgtctgg tgaagggctt ctacccttcc gacatcgccg tggagtggga gtctaacggc     1740

cagccagaga acaattacaa gaccacacct ccagtgctgg actctgatgg cagcttcttt     1800

ctgtattctc ggctgaccgt ggataagagc agatggcagg agggcaacgt gttcagctgc     1860

tccgtgatgc acgaggccct gcacaaccac tatacacaga agtctctgag cctgtccctg     1920

ggcaag                                                                1926


<210>  24
<211>  375
<212>  PRT
<213>  Artificial sequence

<220>
<223>  SIGLEC10 Fc (IgG1 knob) 375 AA first monomer of DSP402, DSP412

<400>  24

Asp Gly Arg Phe Trp Ile Arg Val Gln Glu Ser Val Met Val Pro Glu 
1               5                   10                  15      


Gly Leu Ser Ile Ser Val Pro Cys Ser Phe Ser Tyr Pro Arg Gln Asp 
            20                  25                  30          


Trp Thr Gly Ser Thr Pro Ala Tyr Gly Tyr Trp Phe Lys Ala Val Thr 
        35                  40                  45              


Glu Thr Thr Lys Gly Ala Pro Val Ala Thr Asn His Gln Ser Arg Glu 
    50                  55                  60                  


Val Glu Met Ser Thr Arg Gly Arg Phe Gln Leu Thr Gly Asp Pro Ala 
65                  70                  75                  80  


Lys Gly Asn Cys Ser Leu Val Ile Arg Asp Ala Gln Met Gln Asp Glu 
                85                  90                  95      


Ser Gln Tyr Phe Phe Arg Val Glu Arg Gly Ser Tyr Val Arg Tyr Asn 
            100                 105                 110         


Phe Met Asn Asp Gly Phe Phe Leu Lys Val Thr Ala Leu Thr Gln Lys 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
    130                 135                 140                 


Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro 
145                 150                 155                 160 


Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
                165                 170                 175     


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
            180                 185                 190         


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
        195                 200                 205             


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
    210                 215                 220                 


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
225                 230                 235                 240 


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
                245                 250                 255     


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
            260                 265                 270         


Glu Pro Gln Val Tyr Thr Leu Pro Pro Cys Arg Asp Glu Leu Thr Lys 
        275                 280                 285             


Asn Gln Val Ser Leu Trp Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
    290                 295                 300                 


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
305                 310                 315                 320 


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
                325                 330                 335     


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
            340                 345                 350         


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
        355                 360                 365             


Leu Ser Leu Ser Pro Gly Lys 
    370                 375 


<210>  25
<211>  367
<212>  PRT
<213>  Artificial sequence

<220>
<223>  SIGLEC10 Fc (IgG4) 367 AA first monomer of DSP402V1, DSP412V1

<400>  25

Asp Gly Arg Phe Trp Ile Arg Val Gln Glu Ser Val Met Val Pro Glu 
1               5                   10                  15      


Gly Leu Ser Ile Ser Val Pro Cys Ser Phe Ser Tyr Pro Arg Gln Asp 
            20                  25                  30          


Trp Thr Gly Ser Thr Pro Ala Tyr Gly Tyr Trp Phe Lys Ala Val Thr 
        35                  40                  45              


Glu Thr Thr Lys Gly Ala Pro Val Ala Thr Asn His Gln Ser Arg Glu 
    50                  55                  60                  


Val Glu Met Ser Thr Arg Gly Arg Phe Gln Leu Thr Gly Asp Pro Ala 
65                  70                  75                  80  


Lys Gly Asn Cys Ser Leu Val Ile Arg Asp Ala Gln Met Gln Asp Glu 
                85                  90                  95      


Ser Gln Tyr Phe Phe Arg Val Glu Arg Gly Ser Tyr Val Arg Tyr Asn 
            100                 105                 110         


Phe Met Asn Asp Gly Phe Phe Leu Lys Val Thr Ala Leu Thr Gln Lys 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ser Lys Tyr Gly Pro 
    130                 135                 140                 


Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Glu Gly Gly Pro Ser Val 
145                 150                 155                 160 


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
                165                 170                 175     


Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu 
            180                 185                 190         


Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
        195                 200                 205             


Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 
    210                 215                 220                 


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
225                 230                 235                 240 


Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile 
                245                 250                 255     


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Cys Thr Leu Pro 
            260                 265                 270         


Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Trp Cys Leu 
        275                 280                 285             


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
    290                 295                 300                 


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
305                 310                 315                 320 


Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg 
                325                 330                 335     


Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
            340                 345                 350         


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys 
        355                 360                 365         


<210>  26
<211>  1101
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA of#25

<400>  26
gatggccggt tttggatcag agtgcaggag tccgtgatgg tgcctgaggg cctgtctatc       60

agcgtgccat gctccttctc ttaccccaga caggactgga ccggctctac acccgcctac      120

ggctattggt ttaaggccgt gaccgagaca acaaagggcg cccctgtggc cacaaaccac      180

cagagcagag aggtggagat gtccacccgg ggcagattcc agctgacagg cgaccccgcc      240

aagggcaatt gtagcctggt catcagggac gcccagatgc aggatgagtc tcagtacttc      300

tttagggtgg agcgcggcag ctacgtgcgc tataacttta tgaatgatgg cttctttctg      360

aaggtgaccg ccctgacaca gaagggagga ggaggctccg gcggaggagg cagcgagtcc      420

aagtatggac caccttgccc accatgtcct gcaccagagt tcgagggagg acctagcgtg      480

ttcctgtttc ctccaaagcc aaaggacacc ctgatgatca gcaggactcc tgaggtgaca      540

tgcgtggtgg tggacgtgtc ccaggaggac cccgaggtgc agttcaactg gtatgtggat      600

ggcgtggagg tgcacaatgc caagacaaag ccacgggagg agcagtttaa ctctacctac      660

agagtggtga gcgtgctgac agtgctgcac caggattggc tgaacggcaa ggagtataag      720

tgtaaggtgt ctaataaggg cctgcccagc tccatcgaga aaaccatcag caaggcaaag      780

ggacagcccc gggagcctca ggtgtgcacc ctgccccctt cccaggagga gatgacaaag      840

aaccaggtgt ctctgtggtg tctggtgaag ggcttctacc caagcgacat cgccgtggag      900

tgggagtcca atggccagcc cgagaacaat tacaagacca caccacccgt gctggactcc      960

gatggctctt tctttctgta ttccaggctg accgtggata agtctcgctg gcaggagggc     1020

aacgtgtttt cttgcagcgt gatgcacgag gccctgcaca atcactatac acagaagtcc     1080

ctgtctctga gcctgggcaa g                                               1101


<210>  27
<211>  232
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hIgG1 Fc linker (knob V1 only in DSP216V2) 232 aa

<400>  27

Glu Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             


Arg Glu Pro Gln Val Cys Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    130                 135                 140                 


Lys Asn Gln Val Ser Leu Trp Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 


Ser Leu Ser Leu Ser Pro Gly Lys 
225                 230         


<210>  28
<211>  232
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hIgG1 Fc linker (hole V1 only in DSP216V2) 232 aa

<400>  28

Glu Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Cys Glu Leu Thr 
    130                 135                 140                 


Lys Asn Gln Val Ser Leu Ser Cys Ala Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Val 
            180                 185                 190         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 


Ser Leu Ser Leu Ser Pro Gly Lys 
225                 230         


<210>  29
<211>  375
<212>  PRT
<213>  Artificial sequence

<220>
<223>  SIGLEC10 Fc (IgG1 hole)  475 AA first monomer of DSP404, DSP403

<400>  29

Asp Gly Arg Phe Trp Ile Arg Val Gln Glu Ser Val Met Val Pro Glu 
1               5                   10                  15      


Gly Leu Ser Ile Ser Val Pro Cys Ser Phe Ser Tyr Pro Arg Gln Asp 
            20                  25                  30          


Trp Thr Gly Ser Thr Pro Ala Tyr Gly Tyr Trp Phe Lys Ala Val Thr 
        35                  40                  45              


Glu Thr Thr Lys Gly Ala Pro Val Ala Thr Asn His Gln Ser Arg Glu 
    50                  55                  60                  


Val Glu Met Ser Thr Arg Gly Arg Phe Gln Leu Thr Gly Asp Pro Ala 
65                  70                  75                  80  


Lys Gly Asn Cys Ser Leu Val Ile Arg Asp Ala Gln Met Gln Asp Glu 
                85                  90                  95      


Ser Gln Tyr Phe Phe Arg Val Glu Arg Gly Ser Tyr Val Arg Tyr Asn 
            100                 105                 110         


Phe Met Asn Asp Gly Phe Phe Leu Lys Val Thr Ala Leu Thr Gln Lys 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
    130                 135                 140                 


Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro 
145                 150                 155                 160 


Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
                165                 170                 175     


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
            180                 185                 190         


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
        195                 200                 205             


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
    210                 215                 220                 


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
225                 230                 235                 240 


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
                245                 250                 255     


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
            260                 265                 270         


Glu Pro Gln Val Cys Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys 
        275                 280                 285             


Asn Gln Val Ser Leu Ser Cys Ala Val Lys Gly Phe Tyr Pro Ser Asp 
    290                 295                 300                 


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
305                 310                 315                 320 


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Val Ser 
                325                 330                 335     


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
            340                 345                 350         


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
        355                 360                 365             


Leu Ser Leu Ser Pro Gly Lys 
    370                 375 


<210>  30
<211>  369
<212>  PRT
<213>  Artificial sequence

<220>
<223>  SIGLEC10 Fc (IgG4 hole) second monomer of DSP404V1, DSP403V1

<400>  30

Met Thr Gly Thr Ile Glu Thr Thr Gly Asn Ile Ser Ala Glu Lys Gly 
1               5                   10                  15      


Gly Ser Ile Ala Leu Gln Cys His Leu Ser Ser Thr Thr Ala Gln Val 
            20                  25                  30          


Thr Gln Val Asn Trp Glu Gln Gln Asp Gln Leu Leu Ala Ile Ser Asn 
        35                  40                  45              


Ala Asp Leu Gly Trp His Ile Ser Pro Ser Phe Lys Asp Arg Val Ala 
    50                  55                  60                  


Pro Gly Pro Gly Leu Gly Leu Thr Leu Gln Ser Leu Thr Val Asn Asp 
65                  70                  75                  80  


Thr Gly Glu Tyr Phe Cys Ile Tyr His Thr Tyr Pro Asp Gly Thr Tyr 
                85                  90                  95      


Thr Gly Arg Ile Phe Leu Glu Val Leu Glu Ser Ser Val Ala Glu His 
            100                 105                 110         


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Pro Lys Ser Ser Asp 
        115                 120                 125             


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Glu Ser Lys Tyr 
    130                 135                 140                 


Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Glu Gly Gly Pro 
145                 150                 155                 160 


Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 
                165                 170                 175     


Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp 
            180                 185                 190         


Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn 
        195                 200                 205             


Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val 
    210                 215                 220                 


Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu 
225                 230                 235                 240 


Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys 
                245                 250                 255     


Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
            260                 265                 270         


Leu Pro Pro Ser Gln Cys Glu Met Thr Lys Asn Gln Val Ser Leu Ser 
        275                 280                 285             


Cys Ala Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
    290                 295                 300                 


Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
305                 310                 315                 320 


Asp Ser Asp Gly Ser Phe Phe Leu Val Ser Arg Leu Thr Val Asp Lys 
                325                 330                 335     


Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
            340                 345                 350         


Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly 
        355                 360                 365             


Lys 
    


<210>  31
<211>  351
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TIGIT No I42D Fc (IgG4 knob) 351 AA first monomer of DSP502V2

<400>  31

Met Thr Gly Thr Ile Glu Thr Thr Gly Asn Ile Ser Ala Glu Lys Gly 
1               5                   10                  15      


Gly Ser Ile Ile Leu Gln Cys His Leu Ser Ser Thr Thr Ala Gln Val 
            20                  25                  30          


Thr Gln Val Asn Trp Glu Gln Gln Asp Gln Leu Leu Ala Ile Ser Asn 
        35                  40                  45              


Ala Asp Leu Gly Trp His Ile Ser Pro Ser Phe Lys Asp Arg Val Ala 
    50                  55                  60                  


Pro Gly Pro Gly Leu Gly Leu Thr Leu Gln Ser Leu Thr Val Asn Asp 
65                  70                  75                  80  


Thr Gly Glu Tyr Phe Cys Ile Tyr His Thr Tyr Pro Asp Gly Thr Tyr 
                85                  90                  95      


Thr Gly Arg Ile Phe Leu Glu Val Leu Glu Ser Ser Val Ala Glu His 
            100                 105                 110         


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ser Lys Tyr Gly Pro 
        115                 120                 125             


Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Glu Gly Gly Pro Ser Val 
    130                 135                 140                 


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
145                 150                 155                 160 


Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu 
                165                 170                 175     


Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
            180                 185                 190         


Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 
        195                 200                 205             


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
    210                 215                 220                 


Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile 
225                 230                 235                 240 


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Cys Thr Leu Pro 
                245                 250                 255     


Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Trp Cys Leu 
            260                 265                 270         


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
        275                 280                 285             


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
    290                 295                 300                 


Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg 
305                 310                 315                 320 


Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
                325                 330                 335     


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys 
            340                 345                 350     


<210>  32
<211>  1053
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA of#31

<400>  32
atgaccggca caatcgagac aacaggcaac atctctgccg agaagggagg cagcatcatc       60

ctgcagtgcc acctgagcag caccacagcc caggtgaccc aggtgaactg ggagcagcag      120

gaccagctgc tggccatctc caatgccgat ctgggctggc acatcagccc ctcctttaag      180

gatagggtgg cacctggacc aggcctgggc ctgaccctgc agagcctgac cgtgaatgac      240

acaggcgagt acttctgtat ctaccacaca tatcctgatg gcacctatac aggcagaatc      300

tttctggagg tgctggagtc tagcgtggcc gagcacggag gaggaggctc cggaggagga      360

ggctctgaga gcaagtacgg accaccttgc ccaccatgtc cagcacctga gttcgaggga      420

ggacctagcg tgttcctgtt tcctccaaag ccaaaggaca ccctgatgat cagcaggacc      480

cctgaggtga catgcgtggt ggtggacgtg tcccaggagg accccgaggt gcagttcaac      540

tggtatgtgg atggcgtgga ggtgcacaat gccaagacaa agcccaggga ggagcagttt      600

aactccacct accgcgtggt gtctgtgctg acagtgctgc accaggactg gctgaacggc      660

aaggagtata agtgtaaggt gtctaataag ggcctgccct cctctatcga gaaaaccatc      720

agcaaggcca agggccagcc aagagagcca caggtgtgca ccctgccacc ttcccaggag      780

gagatgacaa agaaccaggt gtctctgtgg tgtctggtga agggcttcta cccatctgac      840

atcgccgtgg agtgggagag caatggccag cccgagaaca attacaagac cacaccaccc      900

gtgctggaca gcgatggctc cttctttctg tatagccggc tgaccgtgga taagtccaga      960

tggcaggagg gcaacgtgtt ttcctgctct gtgatgcacg aggccctgca caatcactat     1020

acacagaaga gcctgtccct gtctctgggc aag                                  1053


<210>  33
<211>  355
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TIGIT WT Fc (IgG4 knob) 355AA first monomer of DSP502V3

<400>  33

Met Met Thr Gly Thr Ile Glu Thr Thr Gly Asn Ile Ser Ala Glu Lys 
1               5                   10                  15      


Gly Gly Ser Ile Ile Leu Gln Cys His Leu Ser Ser Thr Thr Ala Gln 
            20                  25                  30          


Val Thr Gln Val Asn Trp Glu Gln Gln Asp Gln Leu Leu Ala Ile Cys 
        35                  40                  45              


Asn Ala Asp Leu Gly Trp His Ile Ser Pro Ser Phe Lys Asp Arg Val 
    50                  55                  60                  


Ala Pro Gly Pro Gly Leu Gly Leu Thr Leu Gln Ser Leu Thr Val Asn 
65                  70                  75                  80  


Asp Thr Gly Glu Tyr Phe Cys Ile Tyr His Thr Tyr Pro Asp Gly Thr 
                85                  90                  95      


Tyr Thr Gly Arg Ile Phe Leu Glu Val Leu Glu Ser Ser Val Ala Glu 
            100                 105                 110         


His Gly Ala Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ser 
        115                 120                 125             


Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Glu Gly 
    130                 135                 140                 


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
145                 150                 155                 160 


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln 
                165                 170                 175     


Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
            180                 185                 190         


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr 
        195                 200                 205             


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
    210                 215                 220                 


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile 
225                 230                 235                 240 


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
                245                 250                 255     


Cys Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser 
            260                 265                 270         


Leu Trp Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
        275                 280                 285             


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
    290                 295                 300                 


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val 
305                 310                 315                 320 


Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met 
                325                 330                 335     


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
            340                 345                 350         


Leu Gly Lys 
        355 


<210>  34
<211>  1065
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NA of #33

<400>  34
atgatgaccg gcactattga aactaccggc aacatctctg ccgagaaggg cggcagcatc       60

atcctccagt gccacctgag cagcaccaca gcccaggtga cacaggtgaa ctgggagcag      120

caggaccagc tgctggccat ctgtaatgcc gatctgggct ggcacatcag cccttccttc      180

aaggacaggg tggcccctgg cccaggcctg ggcctgaccc tccagagcct gaccgtgaat      240

gacacaggcg agtacttctg catctaccac acatatccag atggcaccta tacaggccgg      300

atctttctgg aggtgctgga gtctagcgtg gcagagcacg gcgccagagg cggaggaggc      360

agcggaggag gaggctctga gagcaagtac ggccctcctt gcccaccatg tccagcacct      420

gagtttgagg gcggcccttc cgtgttcctg tttcctccaa agccaaagga caccctgatg      480

atcagcagga ccccagaggt gacatgcgtg gtggtggacg tgtcccagga ggaccccgag      540

gtgcagttca actggtatgt ggatggcgtg gaggtgcaca atgccaagac aaagcccagg      600

gaggagcagt ttaactccac ctaccgcgtg gtgtctgtgc tgacagtgct gcaccaggat      660

tggctgaacg gcaaggagta taagtgtaag gtgtctaata agggcctgcc ttcctctatc      720

gagaaaacca tcagcaaggc aaagggacag ccacgcgagc cacaggtgtg caccctgccc      780

ccttcccagg aggagatgac aaagaaccag gtgtctctgt ggtgtctggt gaagggcttc      840

tacccctctg acatcgccgt ggagtgggag agcaatggcc agcctgagaa caattacaag      900

accacaccac ccgtgctgga cagcgatggc tccttctttc tgtatagccg gctgaccgtg      960

gataagtcca gatggcagga gggcaacgtg ttcagctgct ccgtgatgca cgaagcactg     1020

cacaatcatt acactcagaa gtccctgtcc ctgtcactgg gcaag                     1065


<210>  35
<211>  354
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TIGIT Fc (IgG1 Hole) 354 AA second monomer of DSP503

<400>  35

Met Thr Gly Thr Ile Glu Thr Thr Gly Asn Ile Ser Ala Glu Lys Gly 
1               5                   10                  15      


Gly Ser Ile Ala Leu Gln Cys His Leu Ser Ser Thr Thr Ala Gln Val 
            20                  25                  30          


Thr Gln Val Asn Trp Glu Gln Gln Asp Gln Leu Leu Ala Ile Ser Asn 
        35                  40                  45              


Ala Asp Leu Gly Trp His Ile Ser Pro Ser Phe Lys Asp Arg Val Ala 
    50                  55                  60                  


Pro Gly Pro Gly Leu Gly Leu Thr Leu Gln Ser Leu Thr Val Asn Asp 
65                  70                  75                  80  


Thr Gly Glu Tyr Phe Cys Ile Tyr His Thr Tyr Pro Asp Gly Thr Tyr 
                85                  90                  95      


Thr Gly Arg Ile Phe Leu Glu Val Leu Glu Ser Ser Val Ala Glu His 
            100                 105                 110         


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Pro Lys Ser Ser Asp 
        115                 120                 125             


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly 
    130                 135                 140                 


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
145                 150                 155                 160 


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
                165                 170                 175     


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
            180                 185                 190         


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 
        195                 200                 205             


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
    210                 215                 220                 


Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
225                 230                 235                 240 


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Cys 
                245                 250                 255     


Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu 
            260                 265                 270         


Ser Cys Ala Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
        275                 280                 285             


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
    290                 295                 300                 


Leu Asp Ser Asp Gly Ser Phe Phe Leu Val Ser Lys Leu Thr Val Asp 
305                 310                 315                 320 


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
                325                 330                 335     


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
            340                 345                 350         


Gly Lys 
        


<210>  36
<211>  351
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TIGIT Fc (IgG4 Hole) 351 AA second monomer of DSP503V1

<400>  36

Met Thr Gly Thr Ile Glu Thr Thr Gly Asn Ile Ser Ala Glu Lys Gly 
1               5                   10                  15      


Gly Ser Ile Ala Leu Gln Cys His Leu Ser Ser Thr Thr Ala Gln Val 
            20                  25                  30          


Thr Gln Val Asn Trp Glu Gln Gln Asp Gln Leu Leu Ala Ile Ser Asn 
        35                  40                  45              


Ala Asp Leu Gly Trp His Ile Ser Pro Ser Phe Lys Asp Arg Val Ala 
    50                  55                  60                  


Pro Gly Pro Gly Leu Gly Leu Thr Leu Gln Ser Leu Thr Val Asn Asp 
65                  70                  75                  80  


Thr Gly Glu Tyr Phe Cys Ile Tyr His Thr Tyr Pro Asp Gly Thr Tyr 
                85                  90                  95      


Thr Gly Arg Ile Phe Leu Glu Val Leu Glu Ser Ser Val Ala Glu His 
            100                 105                 110         


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ser Lys Tyr Gly Pro 
        115                 120                 125             


Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Glu Gly Gly Pro Ser Val 
    130                 135                 140                 


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
145                 150                 155                 160 


Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu 
                165                 170                 175     


Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
            180                 185                 190         


Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 
        195                 200                 205             


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
    210                 215                 220                 


Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile 
225                 230                 235                 240 


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
                245                 250                 255     


Pro Ser Gln Cys Glu Met Thr Lys Asn Gln Val Ser Leu Ser Cys Ala 
            260                 265                 270         


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
        275                 280                 285             


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
    290                 295                 300                 


Asp Gly Ser Phe Phe Leu Val Ser Arg Leu Thr Val Asp Lys Ser Arg 
305                 310                 315                 320 


Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
                325                 330                 335     


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys 
            340                 345                 350     


<210>  37
<211>  288
<212>  PRT
<213>  Artificial sequence

<220>
<223>  aa sequence of full length PD1

<400>  37

Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      


Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          


Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             


Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 


Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 


Arg Pro Ala Gly Gln Phe Gln Thr Leu Val Val Gly Val Val Gly Gly 
                165                 170                 175     


Leu Leu Gly Ser Leu Val Leu Leu Val Trp Val Leu Ala Val Ile Cys 
            180                 185                 190         


Ser Arg Ala Ala Arg Gly Thr Ile Gly Ala Arg Arg Thr Gly Gln Pro 
        195                 200                 205             


Leu Lys Glu Asp Pro Ser Ala Val Pro Val Phe Ser Val Asp Tyr Gly 
    210                 215                 220                 


Glu Leu Asp Phe Gln Trp Arg Glu Lys Thr Pro Glu Pro Pro Val Pro 
225                 230                 235                 240 


Cys Val Pro Glu Gln Thr Glu Tyr Ala Thr Ile Val Phe Pro Ser Gly 
                245                 250                 255     


Met Gly Thr Ser Ser Pro Ala Arg Arg Gly Ser Ala Asp Gly Pro Arg 
            260                 265                 270         


Ser Ala Gln Pro Leu Arg Pro Glu Asp Gly His Cys Ser Trp Pro Leu 
        275                 280                 285             


<210>  38
<211>  867
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  na sequence of full length PD1

<400>  38
atgcagatcc cacaggcgcc ctggccagtc gtctgggcgg tgctacaact gggctggcgg       60

ccaggatggt tcttagactc cccagacagg ccctggaacc cccccacctt ctccccagcc      120

ctgctcgtgg tgaccgaagg ggacaacgcc accttcacct gcagcttctc caacacatcg      180

gagagcttcg tgctaaactg gtaccgcatg agccccagca accagacgga caagctggcc      240

gccttccccg aggaccgcag ccagcccggc caggactgcc gcttccgtgt cacacaactg      300

cccaacgggc gtgacttcca catgagcgtg gtcagggccc ggcgcaatga cagcggcacc      360

tacctctgtg gggccatctc cctggccccc aaggcgcaga tcaaagagag cctgcgggca      420

gagctcaggg tgacagagag aagggcagaa gtgcccacag cccaccccag cccctcaccc      480

aggccagccg gccagttcca aaccctggtg gttggtgtcg tgggcggcct gctgggcagc      540

ctggtgctgc tagtctgggt cctggccgtc atctgctccc gggccgcacg agggacaata      600

ggagccaggc gcaccggcca gcccctgaag gaggacccct cagccgtgcc tgtgttctct      660

gtggactatg gggagctgga tttccagtgg cgagagaaga ccccggagcc ccccgtgccc      720

tgtgtccctg agcagacgga gtatgccacc attgtctttc ctagcggaat gggcacctca      780

tcccccgccc gcaggggctc agctgacggc cctcggagtg cccagccact gaggcctgag      840

gatggacact gctcttggcc cctctga                                          867


<210>  39
<211>  150
<212>  PRT
<213>  Artificial sequence

<220>
<223>  PD1 ECD Full with CYS93>Ser substitution

<400>  39

Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp Asn Pro Pro Thr 
1               5                   10                  15      


Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp Asn Ala Thr Phe 
            20                  25                  30          


Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val Leu Asn Trp Tyr 
        35                  40                  45              


Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala Ala Phe Pro Glu 
    50                  55                  60                  


Asp Arg Ser Gln Pro Gly Gln Asp Ser Arg Phe Arg Val Thr Gln Leu 
65                  70                  75                  80  


Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg Ala Arg Arg Asn 
                85                  90                  95      


Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu Ala Pro Lys Ala 
            100                 105                 110         


Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val Thr Glu Arg Arg 
        115                 120                 125             


Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro Arg Pro Ala Gly 
    130                 135                 140                 


Gln Phe Gln Thr Leu Val 
145                 150 


<210>  40
<211>  450
<212>  DNA
<213>  Artificial sequence

<220>
<223>  na sequence encoding SEQ ID NO:39

<400>  40
cccggctggt ttctggactc tccagacaga ccttggaacc ctccaacctt ctctcccgct       60

ctgctggtgg ttaccgaggg cgacaatgcc accttcacct gttccttcag caacacctcc      120

gagtccttcg tgctgaactg gtacagaatg tcccctagca accagaccga caagctggcc      180

gcctttcctg aggacagatc tcagccaggc caggactctc ggttcagagt tacccagctg      240

cctaacggcc gggacttcca catgtctgtt gtgcgggcca gacggaacga ctctggcaca      300

tatctgtgcg gcgccatctc tctggctccc aaggctcaga tcaaagagtc tctgcgggcc      360

gagctgagag tgacagaaag acgagctgag gtgcccaccg ctcatccctc accttctcca      420

agacctgctg gccagtttca gacactcgtg                                       450


<210>  41
<211>  167
<212>  PRT
<213>  Artificial sequence

<220>
<223>  PD1 ECM KNOWN FRAGMENT

<400>  41

Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      


Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          


Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             


Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 


Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 


Arg Pro Ala Gly Gln Phe Gln 
                165         


<210>  42
<211>  122
<212>  PRT
<213>  Artificial sequence

<220>
<223>  PD1 ECM KNOWN FRAGMENT

<400>  42

Asp Ser Pro Asp Arg Pro Trp Asn Pro Pro Thr Phe Ser Pro Ala Leu 
1               5                   10                  15      


Leu Val Val Thr Glu Gly Asp Asn Ala Thr Phe Thr Cys Ser Phe Ser 
            20                  25                  30          


Asn Thr Ser Glu Ser Phe Val Leu Asn Trp Tyr Arg Met Ser Pro Ser 
        35                  40                  45              


Asn Gln Thr Asp Lys Leu Ala Ala Phe Pro Glu Asp Arg Ser Gln Pro 
    50                  55                  60                  


Gly Gln Asp Cys Arg Phe Arg Val Thr Gln Leu Pro Asn Gly Arg Asp 
65                  70                  75                  80  


Phe His Met Ser Val Val Arg Ala Arg Arg Asn Asp Ser Gly Thr Tyr 
                85                  90                  95      


Leu Cys Gly Ala Ile Ser Leu Ala Pro Lys Ala Gln Ile Lys Glu Ser 
            100                 105                 110         


Leu Arg Ala Glu Leu Arg Val Thr Glu Arg 
        115                 120         


<210>  43
<211>  150
<212>  PRT
<213>  Artificial sequence

<220>
<223>  150 aa ORIGINAL PD1 DOMAIN

<400>  43

Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp Asn Pro Pro Thr 
1               5                   10                  15      


Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp Asn Ala Thr Phe 
            20                  25                  30          


Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val Leu Asn Trp Tyr 
        35                  40                  45              


Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala Ala Phe Pro Glu 
    50                  55                  60                  


Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg Val Thr Gln Leu 
65                  70                  75                  80  


Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg Ala Arg Arg Asn 
                85                  90                  95      


Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu Ala Pro Lys Ala 
            100                 105                 110         


Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val Thr Glu Arg Arg 
        115                 120                 125             


Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro Arg Pro Ala Gly 
    130                 135                 140                 


Gln Phe Gln Thr Leu Val 
145                 150 


<210>  44
<211>  450
<212>  DNA
<213>  Artificial sequence

<220>
<223>  na pf SEQ ID NO 43

<400>  44
ccaggatggt tcttagactc tccagatagg ccttggaatc cccctacctt tagccccgcc       60

ctgctggtgg tgacagaggg cgataacgcc accttcacat gctcttttag caacacctcc      120

gagtctttcg tgctgaattg gtacaggatg agcccttcca accagacaga caagctggca      180

gcatttcctg aggaccgctc ccagccaggc caggattgcc ggttcagagt gacccagctg      240

ccaaatggca gggactttca catgagcgtg gtgcgcgccc ggagaaacga ttccggcaca      300

tacctgtgcg gagcaatctc tctggcacca aaggcacaga tcaaggagtc cctgagggca      360

gagctgaggg tgaccgagag gagggccgag gtgccaacag cacacccatc tcctagccca      420

aggccagcag gacagttcca aaccctggtg                                       450


<210>  45
<211>  150
<212>  PRT
<213>  Artificial sequence

<220>
<223>  150 aa ORIGINAL PD1 DOMAIN with CYS93>Ser substitution

<400>  45

Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp Asn Pro Pro Thr 
1               5                   10                  15      


Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp Asn Ala Thr Phe 
            20                  25                  30          


Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val Leu Asn Trp Tyr 
        35                  40                  45              


Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala Ala Phe Pro Glu 
    50                  55                  60                  


Asp Arg Ser Gln Pro Gly Gln Asp Ser Arg Phe Arg Val Thr Gln Leu 
65                  70                  75                  80  


Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg Ala Arg Arg Asn 
                85                  90                  95      


Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu Ala Pro Lys Ala 
            100                 105                 110         


Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val Thr Glu Arg Arg 
        115                 120                 125             


Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro Arg Pro Ala Gly 
    130                 135                 140                 


Gln Phe Gln Thr Leu Val 
145                 150 


<210>  46
<211>  450
<212>  DNA
<213>  Artificial sequence

<220>
<223>  na of SEQ ID NO  45

<400>  46
ccaggatggt tcttagactc tccagatagg ccttggaatc cccctacctt tagccccgcc       60

ctgctggtgg tgacagaggg cgataacgcc accttcacat gctcttttag caacacctcc      120

gagtctttcg tgctgaattg gtacaggatg agcccttcca accagacaga caagctggca      180

gcatttcctg aggaccgctc ccagccaggc caggattctc ggttcagagt gacccagctg      240

ccaaatggca gggactttca catgagcgtg gtgcgcgccc ggagaaacga ttccggcaca      300

tacctgtgcg gagcaatctc tctggcacca aaggcacaga tcaaggagtc cctgagggca      360

gagctgaggg tgaccgagag gagggccgag gtgccaacag cacacccatc tcctagccca      420

aggccagcag gacagttcca aaccctggtg                                       450


<210>  47
<211>  140
<212>  PRT
<213>  Artificial sequence

<220>
<223>  140 aa -5-5 in PD1 DOMAIN

<400>  47

Asp Ser Pro Asp Arg Pro Trp Asn Pro Pro Thr Phe Ser Pro Ala Leu 
1               5                   10                  15      


Leu Val Val Thr Glu Gly Asp Asn Ala Thr Phe Thr Cys Ser Phe Ser 
            20                  25                  30          


Asn Thr Ser Glu Ser Phe Val Leu Asn Trp Tyr Arg Met Ser Pro Ser 
        35                  40                  45              


Asn Gln Thr Asp Lys Leu Ala Ala Phe Pro Glu Asp Arg Ser Gln Pro 
    50                  55                  60                  


Gly Gln Asp Cys Arg Phe Arg Val Thr Gln Leu Pro Asn Gly Arg Asp 
65                  70                  75                  80  


Phe His Met Ser Val Val Arg Ala Arg Arg Asn Asp Ser Gly Thr Tyr 
                85                  90                  95      


Leu Cys Gly Ala Ile Ser Leu Ala Pro Lys Ala Gln Ile Lys Glu Ser 
            100                 105                 110         


Leu Arg Ala Glu Leu Arg Val Thr Glu Arg Arg Ala Glu Val Pro Thr 
        115                 120                 125             


Ala His Pro Ser Pro Ser Pro Arg Pro Ala Gly Gln 
    130                 135                 140 


<210>  48
<211>  419
<212>  DNA
<213>  Artificial sequence

<220>
<223>  na of SEQ ID NO 47

<400>  48
gactctccag ataggccttg gaatccccct acctttagcc ccgccctgct ggtggtgaca       60

gagggcgata acgccacctt cacatgctct tttagcaaca cctccgagtc tttcgtgctg      120

aattggtaca ggatgagccc ttccaaccag acagacaagc tggcagcatt tcctgaggac      180

cgctcccagc caggccagga ttgccggttc agagtgaccc agctgccaaa tggcagggac      240

tttcacatga gcgtggtgcg cgcccggaga aacgattccg gcacatacct gtgcggagca      300

atctctctgg caccaaaggc acagatcaag gagtccctga gggcagagct gagggtgacc      360

gagaggaggg ccgaggtgcc aacagcacac ccatctccta gcccaaggcc agcaggaca       419


<210>  49
<211>  140
<212>  PRT
<213>  Artificial sequence

<220>
<223>  140 aa -5-5 in PD1 DOMAIN with CYS93>Ser substitution

<400>  49

Asp Ser Pro Asp Arg Pro Trp Asn Pro Pro Thr Phe Ser Pro Ala Leu 
1               5                   10                  15      


Leu Val Val Thr Glu Gly Asp Asn Ala Thr Phe Thr Cys Ser Phe Ser 
            20                  25                  30          


Asn Thr Ser Glu Ser Phe Val Leu Asn Trp Tyr Arg Met Ser Pro Ser 
        35                  40                  45              


Asn Gln Thr Asp Lys Leu Ala Ala Phe Pro Glu Asp Arg Ser Gln Pro 
    50                  55                  60                  


Gly Gln Asp Ser Arg Phe Arg Val Thr Gln Leu Pro Asn Gly Arg Asp 
65                  70                  75                  80  


Phe His Met Ser Val Val Arg Ala Arg Arg Asn Asp Ser Gly Thr Tyr 
                85                  90                  95      


Leu Cys Gly Ala Ile Ser Leu Ala Pro Lys Ala Gln Ile Lys Glu Ser 
            100                 105                 110         


Leu Arg Ala Glu Leu Arg Val Thr Glu Arg Arg Ala Glu Val Pro Thr 
        115                 120                 125             


Ala His Pro Ser Pro Ser Pro Arg Pro Ala Gly Gln 
    130                 135                 140 


<210>  50
<211>  419
<212>  DNA
<213>  Artificial sequence

<220>
<223>  na of SEQ ID NO 49

<400>  50
gactctccag ataggccttg gaatccccct acctttagcc ccgccctgct ggtggtgaca       60

gagggcgata acgccacctt cacatgctct tttagcaaca cctccgagtc tttcgtgctg      120

aattggtaca ggatgagccc ttccaaccag acagacaagc tggcagcatt tcctgaggac      180

cgctcccagc caggccagga tctccggttc agagtgaccc agctgccaaa tggcagggac      240

tttcacatga gcgtggtgcg cgcccggaga aacgattccg gcacatacct gtgcggagca      300

atctctctgg caccaaaggc acagatcaag gagtccctga gggcagagct gagggtgacc      360

gagaggaggg ccgaggtgcc aacagcacac ccatctccta gcccaaggcc agcaggaca       419


<210>  51
<211>  232
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hIgG1 Fc linker (knob) 232 aa

<400>  51

Glu Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Cys Arg Asp Glu Leu Thr 
    130                 135                 140                 


Lys Asn Gln Val Ser Leu Trp Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 


Ser Leu Ser Leu Ser Pro Gly Lys 
225                 230         


<210>  52
<211>  232
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hIgG1 Fc linker (hole) 232 aa

<400>  52

Glu Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             


Arg Glu Pro Gln Val Cys Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    130                 135                 140                 


Lys Asn Gln Val Ser Leu Ser Cys Ala Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Val 
            180                 185                 190         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 


Ser Leu Ser Leu Ser Pro Gly Lys 
225                 230         


<210>  53
<211>  128
<212>  PRT
<213>  Artificial sequence

<220>
<223>  128 aa PD1 segment

<400>  53

Pro Trp Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu 
1               5                   10                  15      


Gly Asp Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser 
            20                  25                  30          


Phe Val Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys 
        35                  40                  45              


Leu Ala Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg 
    50                  55                  60                  


Phe Arg Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val 
65                  70                  75                  80  


Val Arg Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile 
                85                  90                  95      


Ser Leu Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu 
            100                 105                 110         


Arg Val Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro 
        115                 120                 125             


<210>  54
<211>  384
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA OF #53

<400>  54
ccttggaacc ctccaacctt ctctcccgct ctgctggtgg ttaccgaggg cgacaatgcc       60

accttcacct gttccttcag caacacctcc gagtccttcg tgctgaactg gtacagaatg      120

tcccctagca accagaccga caagctggcc gcctttcctg aggacagatc tcagccaggc      180

caggactgcc ggttcagagt tacccagctg cctaacggcc gggacttcca catgtctgtt      240

gtgcgggcca gacggaacga ctctggcaca tatctgtgcg gcgccatctc tctggctccc      300

aaggctcaga tcaaagagtc tctgcgggcc gagctgagag tgacagaaag acgagctgag      360

gtgcccaccg ctcatccctc acct                                             384


<210>  55
<211>  135
<212>  PRT
<213>  Artificial sequence

<220>
<223>  135 aa PD1 segment

<400>  55

Pro Trp Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu 
1               5                   10                  15      


Gly Asp Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser 
            20                  25                  30          


Phe Val Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys 
        35                  40                  45              


Leu Ala Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg 
    50                  55                  60                  


Phe Arg Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val 
65                  70                  75                  80  


Val Arg Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile 
                85                  90                  95      


Ser Leu Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu 
            100                 105                 110         


Arg Val Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro 
        115                 120                 125             


Ser Pro Arg Pro Ala Gly Gln 
    130                 135 


<210>  56
<211>  405
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA OF #55

<400>  56
ccttggaacc ctccaacctt ctctcccgct ctgctggtgg ttaccgaggg cgacaatgcc       60

accttcacct gttccttcag caacacctcc gagtccttcg tgctgaactg gtacagaatg      120

tcccctagca accagaccga caagctggcc gcctttcctg aggacagatc tcagccaggc      180

caggactgcc ggttcagagt tacccagctg cctaacggcc gggacttcca catgtctgtt      240

gtgcgggcca gacggaacga ctctggcaca tatctgtgcg gcgccatctc tctggctccc      300

aaggctcaga tcaaagagtc tctgcgggcc gagctgagag tgacagaaag acgagctgag      360

gtgcccaccg ctcatccctc accttctcca agacctgccg gccag                      405


<210>  57
<211>  133
<212>  PRT
<213>  Artificial sequence

<220>
<223>  133 aa PD1 segment

<400>  57

Asp Ser Pro Asp Arg Pro Trp Asn Pro Pro Thr Phe Ser Pro Ala Leu 
1               5                   10                  15      


Leu Val Val Thr Glu Gly Asp Asn Ala Thr Phe Thr Cys Ser Phe Ser 
            20                  25                  30          


Asn Thr Ser Glu Ser Phe Val Leu Asn Trp Tyr Arg Met Ser Pro Ser 
        35                  40                  45              


Asn Gln Thr Asp Lys Leu Ala Ala Phe Pro Glu Asp Arg Ser Gln Pro 
    50                  55                  60                  


Gly Gln Asp Cys Arg Phe Arg Val Thr Gln Leu Pro Asn Gly Arg Asp 
65                  70                  75                  80  


Phe His Met Ser Val Val Arg Ala Arg Arg Asn Asp Ser Gly Thr Tyr 
                85                  90                  95      


Leu Cys Gly Ala Ile Ser Leu Ala Pro Lys Ala Gln Ile Lys Glu Ser 
            100                 105                 110         


Leu Arg Ala Glu Leu Arg Val Thr Glu Arg Arg Ala Glu Val Pro Thr 
        115                 120                 125             


Ala His Pro Ser Pro 
    130             


<210>  58
<211>  399
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA OF #57

<400>  58
gactcccctg acagaccttg gaaccctcca accttctctc ccgctctgct ggtggttacc       60

gagggcgaca atgccacctt cacctgttcc ttcagcaaca cctccgagtc cttcgtgctg      120

aactggtaca gaatgtcccc tagcaaccag accgacaagc tggccgcctt tcctgaggac      180

agatctcagc caggccagga ctgccggttc agagttaccc agctgcctaa cggccgggac      240

ttccacatgt ctgttgtgcg ggccagacgg aacgactctg gcacatatct gtgcggcgcc      300

atctctctgg ctcccaaggc tcagatcaaa gagtctctgc gggccgagct gagagtgaca      360

gaaagacgag ctgaggtgcc caccgctcat ccctcacct                             399


<210>  59
<211>  135
<212>  PRT
<213>  Artificial sequence

<220>
<223>  PD1 -10-5 (with CYS93>Ser substitution)

<400>  59

Pro Trp Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu 
1               5                   10                  15      


Gly Asp Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser 
            20                  25                  30          


Phe Val Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys 
        35                  40                  45              


Leu Ala Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Ser Arg 
    50                  55                  60                  


Phe Arg Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val 
65                  70                  75                  80  


Val Arg Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile 
                85                  90                  95      


Ser Leu Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu 
            100                 105                 110         


Arg Val Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro 
        115                 120                 125             


Ser Pro Arg Pro Ala Gly Gln 
    130                 135 


<210>  60
<211>  405
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA OF #59

<400>  60
ccttggaacc ctccaacctt ctctcccgct ctgctggtgg ttaccgaggg cgacaatgcc       60

accttcacct gttccttcag caacacctcc gagtccttcg tgctgaactg gtacagaatg      120

tcccctagca accagaccga caagctggcc gcctttcctg aggacagatc tcagccaggc      180

caggactctc ggttcagagt tacccagctg cctaacggcc gggacttcca catgtctgtt      240

gtgcgggcca gacggaacga ctctggcaca tatctgtgcg gcgccatctc tctggctccc      300

aaggctcaga tcaaagagtc tctgcgggcc gagctgagag tgacagaaag acgagctgag      360

gtgcccaccg ctcatccctc accttctcca agacctgctg gccag                      405


<210>  61
<211>  128
<212>  PRT
<213>  Artificial sequence

<220>
<223>  PD1 -10-12 (with CYS93>Ser substitution)

<400>  61

Pro Trp Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu 
1               5                   10                  15      


Gly Asp Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser 
            20                  25                  30          


Phe Val Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys 
        35                  40                  45              


Leu Ala Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Ser Arg 
    50                  55                  60                  


Phe Arg Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val 
65                  70                  75                  80  


Val Arg Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile 
                85                  90                  95      


Ser Leu Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu 
            100                 105                 110         


Arg Val Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro 
        115                 120                 125             


<210>  62
<211>  384
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA OF #61

<400>  62
ccttggaacc ctccaacctt ctctcccgct ctgctggtgg ttaccgaggg cgacaatgcc       60

accttcacct gttccttcag caacacctcc gagtccttcg tgctgaactg gtacagaatg      120

tcccctagca accagaccga caagctggcc gcctttcctg aggacagatc tcagccaggc      180

caggactctc ggttcagagt tacccagctg cctaacggcc gggacttcca catgtctgtt      240

gtgcgggcca gacggaacga ctctggcaca tatctgtgcg gcgccatctc tctggctccc      300

aaggctcaga tcaaagagtc tctgcgggcc gagctgagag tgacagaaag acgagctgag      360

gtgcccaccg ctcatccctc acct                                             384


<210>  63
<211>  133
<212>  PRT
<213>  Artificial sequence

<220>
<223>  PD1 -5-12 (New, from V20) (with CYS93>Ser substitution)

<400>  63

Asp Ser Pro Asp Arg Pro Trp Asn Pro Pro Thr Phe Ser Pro Ala Leu 
1               5                   10                  15      


Leu Val Val Thr Glu Gly Asp Asn Ala Thr Phe Thr Cys Ser Phe Ser 
            20                  25                  30          


Asn Thr Ser Glu Ser Phe Val Leu Asn Trp Tyr Arg Met Ser Pro Ser 
        35                  40                  45              


Asn Gln Thr Asp Lys Leu Ala Ala Phe Pro Glu Asp Arg Ser Gln Pro 
    50                  55                  60                  


Gly Gln Asp Ser Arg Phe Arg Val Thr Gln Leu Pro Asn Gly Arg Asp 
65                  70                  75                  80  


Phe His Met Ser Val Val Arg Ala Arg Arg Asn Asp Ser Gly Thr Tyr 
                85                  90                  95      


Leu Cys Gly Ala Ile Ser Leu Ala Pro Lys Ala Gln Ile Lys Glu Ser 
            100                 105                 110         


Leu Arg Ala Glu Leu Arg Val Thr Glu Arg Arg Ala Glu Val Pro Thr 
        115                 120                 125             


Ala His Pro Ser Pro 
    130             


<210>  64
<211>  399
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA OF #63

<400>  64
gactctccag acagaccttg gaaccctcca accttctctc ccgctctgct ggtggttacc       60

gagggcgaca atgccacctt cacctgttcc ttcagcaaca cctccgagtc cttcgtgctg      120

aactggtaca gaatgtcccc tagcaaccag accgacaagc tggccgcctt tcctgaggac      180

agatctcagc caggccagga ctctcggttc agagttaccc agctgcctaa cggccgggac      240

ttccacatgt ctgttgtgcg ggccagacgg aacgactctg gcacatatct gtgcggcgcc      300

atctctctgg ctcccaaggc tcagatcaaa gagtctctgc gggccgagct gagagtgaca      360

gaaagacgag ctgaggtgcc caccgctcat ccctcacct                             399


<210>  65
<211>  127
<212>  PRT
<213>  Artificial sequence

<220>
<223>  PD1 -11-12

<400>  65

Trp Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly 
1               5                   10                  15      


Asp Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe 
            20                  25                  30          


Val Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu 
        35                  40                  45              


Ala Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe 
    50                  55                  60                  


Arg Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val 
65                  70                  75                  80  


Arg Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser 
                85                  90                  95      


Leu Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg 
            100                 105                 110         


Val Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro 
        115                 120                 125         


<210>  66
<211>  381
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA OF #65

<400>  66
tggaaccctc caaccttctc tcccgctctg ctggtggtta ccgagggcga caatgccacc       60

ttcacctgtt ccttcagcaa cacctccgag tccttcgtgc tgaactggta cagaatgtcc      120

cctagcaacc agaccgacaa gctggccgcc tttcctgagg acagatctca gccaggccag      180

gactgtcggt tcagagtgac ccagctgcct aacggcagag acttccacat gtccgtcgtg      240

cgggccagaa gaaacgactc tggcacctat ctgtgcggcg ccatctctct ggctcccaag      300

gctcagatca aagagtctct gcgggccgag ctgagagtga cagaaagacg agctgaggtg      360

cccaccgctc atccctcacc t                                                381


<210>  67
<211>  127
<212>  PRT
<213>  Artificial sequence

<220>
<223>  PD1 -11-12 (New, from V18) (with CYS93>Ser substitution)

<400>  67

Trp Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly 
1               5                   10                  15      


Asp Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe 
            20                  25                  30          


Val Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu 
        35                  40                  45              


Ala Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Ser Arg Phe 
    50                  55                  60                  


Arg Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val 
65                  70                  75                  80  


Arg Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser 
                85                  90                  95      


Leu Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg 
            100                 105                 110         


Val Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro 
        115                 120                 125         


<210>  68
<211>  381
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA OF #67

<400>  68
tggaaccctc caaccttctc tcccgctctg ctggtggtta ccgagggcga caatgccacc       60

ttcacctgtt ccttcagcaa cacctccgag tccttcgtgc tgaactggta cagaatgtcc      120

cctagcaacc agaccgacaa gctggccgcc tttcctgagg acagatctca gccaggccag      180

gactctcggt tcagagtgac ccagctgcct aacggcagag acttccacat gtccgtcgtg      240

cgggccagaa gaaacgactc tggcacctat ctgtgcggcg ccatctctct ggctcccaag      300

gctcagatca aagagtctct gcgggccgag ctgagagtga cagaaagacg agctgaggtg      360

cccaccgctc atccctcacc t                                                381


<210>  69
<211>  134
<212>  PRT
<213>  Artificial sequence

<220>
<223>  PD1 -11-5

<400>  69

Trp Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly 
1               5                   10                  15      


Asp Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe 
            20                  25                  30          


Val Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu 
        35                  40                  45              


Ala Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe 
    50                  55                  60                  


Arg Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val 
65                  70                  75                  80  


Arg Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser 
                85                  90                  95      


Leu Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg 
            100                 105                 110         


Val Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser 
        115                 120                 125             


Pro Arg Pro Ala Gly Gln 
    130                 


<210>  70
<211>  402
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA OF #69

<400>  70
tggaaccctc caaccttctc tcccgctctg ctggtggtta ccgagggcga caatgccacc       60

ttcacctgtt ccttcagcaa cacctccgag tccttcgtgc tgaactggta cagaatgtcc      120

cctagcaacc agaccgacaa gctggccgcc tttcctgagg acagatctca gccaggccag      180

gactgtcggt tcagagtgac ccagctgcct aacggcagag acttccacat gtccgtcgtg      240

cgggccagaa gaaacgactc tggcacctat ctgtgcggcg ccatctctct ggctcccaag      300

gctcagatca aagagtctct gcgggccgag ctgagagtga cagaaagacg agctgaggtg      360

cccaccgctc atccctcacc ttctccaaga cctgctggcc ag                         402


<210>  71
<211>  134
<212>  PRT
<213>  Artificial sequence

<220>
<223>  PD1 -11-5 (New, from V19) (with CYS93>Ser substitution)

<400>  71

Trp Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly 
1               5                   10                  15      


Asp Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe 
            20                  25                  30          


Val Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu 
        35                  40                  45              


Ala Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Ser Arg Phe 
    50                  55                  60                  


Arg Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val 
65                  70                  75                  80  


Arg Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser 
                85                  90                  95      


Leu Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg 
            100                 105                 110         


Val Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser 
        115                 120                 125             


Pro Arg Pro Ala Gly Gln 
    130                 


<210>  72
<211>  402
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA OF #71

<400>  72
tggaaccctc caaccttctc tcccgctctg ctggtggtta ccgagggcga caatgccacc       60

ttcacctgtt ccttcagcaa cacctccgag tccttcgtgc tgaactggta cagaatgtcc      120

cctagcaacc agaccgacaa gctggccgcc tttcctgagg acagatctca gccaggccag      180

gactctcggt tcagagtgac ccagctgcct aacggcagag acttccacat gtccgtcgtg      240

cgggccagaa gaaacgactc tggcacctat ctgtgcggcg ccatctctct ggctcccaag      300

gctcagatca aagagtctct gcgggccgag ctgagagtga cagaaagacg agctgaggtg      360

cccaccgctc atccctcacc ttctccaaga cctgctggcc ag                         402


<210>  73
<211>  138
<212>  PRT
<213>  Artificial sequence

<220>
<223>  PD1 -5-7

<400>  73

Asp Ser Pro Asp Arg Pro Trp Asn Pro Pro Thr Phe Ser Pro Ala Leu 
1               5                   10                  15      


Leu Val Val Thr Glu Gly Asp Asn Ala Thr Phe Thr Cys Ser Phe Ser 
            20                  25                  30          


Asn Thr Ser Glu Ser Phe Val Leu Asn Trp Tyr Arg Met Ser Pro Ser 
        35                  40                  45              


Asn Gln Thr Asp Lys Leu Ala Ala Phe Pro Glu Asp Arg Ser Gln Pro 
    50                  55                  60                  


Gly Gln Asp Cys Arg Phe Arg Val Thr Gln Leu Pro Asn Gly Arg Asp 
65                  70                  75                  80  


Phe His Met Ser Val Val Arg Ala Arg Arg Asn Asp Ser Gly Thr Tyr 
                85                  90                  95      


Leu Cys Gly Ala Ile Ser Leu Ala Pro Lys Ala Gln Ile Lys Glu Ser 
            100                 105                 110         


Leu Arg Ala Glu Leu Arg Val Thr Glu Arg Arg Ala Glu Val Pro Thr 
        115                 120                 125             


Ala His Pro Ser Pro Ser Pro Arg Pro Ala 
    130                 135             


<210>  74
<211>  414
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA OF #73

<400>  74
gactctccag acagaccttg gaaccctcca accttctctc ccgctctgct ggtggttacc       60

gagggcgaca atgccacctt cacctgttcc ttcagcaaca cctccgagtc cttcgtgctg      120

aactggtaca gaatgtcccc tagcaaccag accgacaagc tggccgcctt tcctgaggac      180

agatctcagc caggccagga ctgtcggttc agagtgaccc agctgcctaa cggcagagac      240

ttccacatgt ccgtcgtgcg ggccagaaga aacgactctg gcacctatct gtgcggcgcc      300

atctctctgg ctcccaaggc tcagatcaaa gagtctctgc gggccgagct gagagtgaca      360

gaaagacgag ctgaggtgcc caccgctcat ccctcacctt ctccaagacc tgct            414


<210>  75
<211>  138
<212>  PRT
<213>  Artificial sequence

<220>
<223>  PD1 -5-7 (New, from V21) (with CYS93>Ser substitution)

<400>  75

Asp Ser Pro Asp Arg Pro Trp Asn Pro Pro Thr Phe Ser Pro Ala Leu 
1               5                   10                  15      


Leu Val Val Thr Glu Gly Asp Asn Ala Thr Phe Thr Cys Ser Phe Ser 
            20                  25                  30          


Asn Thr Ser Glu Ser Phe Val Leu Asn Trp Tyr Arg Met Ser Pro Ser 
        35                  40                  45              


Asn Gln Thr Asp Lys Leu Ala Ala Phe Pro Glu Asp Arg Ser Gln Pro 
    50                  55                  60                  


Gly Gln Asp Ser Arg Phe Arg Val Thr Gln Leu Pro Asn Gly Arg Asp 
65                  70                  75                  80  


Phe His Met Ser Val Val Arg Ala Arg Arg Asn Asp Ser Gly Thr Tyr 
                85                  90                  95      


Leu Cys Gly Ala Ile Ser Leu Ala Pro Lys Ala Gln Ile Lys Glu Ser 
            100                 105                 110         


Leu Arg Ala Glu Leu Arg Val Thr Glu Arg Arg Ala Glu Val Pro Thr 
        115                 120                 125             


Ala His Pro Ser Pro Ser Pro Arg Pro Ala 
    130                 135             


<210>  76
<211>  414
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA OF #75

<400>  76
gactctccag acagaccttg gaaccctcca accttctctc ccgctctgct ggtggttacc       60

gagggcgaca atgccacctt cacctgttcc ttcagcaaca cctccgagtc cttcgtgctg      120

aactggtaca gaatgtcccc tagcaaccag accgacaagc tggccgcctt tcctgaggac      180

agatctcagc caggccagga ctctcggttc agagtgaccc agctgcctaa cggcagagac      240

ttccacatgt ccgtcgtgcg ggccagaaga aacgactctg gcacctatct gtgcggcgcc      300

atctctctgg ctcccaaggc tcagatcaaa gagtctctgc gggccgagct gagagtgaca      360

gaaagacgag ctgaggtgcc caccgctcat ccctcacctt ctccaagacc tgct            414


<210>  77
<211>  136
<212>  PRT
<213>  Artificial sequence

<220>
<223>  PD1 -5-9 from (with out CYS93>Ser substitution)

<400>  77

Asp Ser Pro Asp Arg Pro Trp Asn Pro Pro Thr Phe Ser Pro Ala Leu 
1               5                   10                  15      


Leu Val Val Thr Glu Gly Asp Asn Ala Thr Phe Thr Cys Ser Phe Ser 
            20                  25                  30          


Asn Thr Ser Glu Ser Phe Val Leu Asn Trp Tyr Arg Met Ser Pro Ser 
        35                  40                  45              


Asn Gln Thr Asp Lys Leu Ala Ala Phe Pro Glu Asp Arg Ser Gln Pro 
    50                  55                  60                  


Gly Gln Asp Cys Arg Phe Arg Val Thr Gln Leu Pro Asn Gly Arg Asp 
65                  70                  75                  80  


Phe His Met Ser Val Val Arg Ala Arg Arg Asn Asp Ser Gly Thr Tyr 
                85                  90                  95      


Leu Cys Gly Ala Ile Ser Leu Ala Pro Lys Ala Gln Ile Lys Glu Ser 
            100                 105                 110         


Leu Arg Ala Glu Leu Arg Val Thr Glu Arg Arg Ala Glu Val Pro Thr 
        115                 120                 125             


Ala His Pro Ser Pro Ser Pro Arg 
    130                 135     


<210>  78
<211>  408
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA OF #77

<400>  78
gactctccag acagaccttg gaaccctcca accttctctc ccgctctgct ggtggttacc       60

gagggcgaca atgccacctt cacctgttcc ttcagcaaca cctccgagtc cttcgtgctg      120

aactggtaca gaatgtcccc tagcaaccag accgacaagc tggccgcctt tcctgaggac      180

agatctcagc caggccagga ctgtcggttc agagttaccc agctgcctaa cggccgggac      240

ttccacatgt ctgttgtgcg ggccagacgg aacgactctg gcacatatct gtgcggcgcc      300

atctctctgg ctcccaaggc tcagatcaaa gagtctctgc gggccgagct gagagtgaca      360

gaaagacgag ctgaggtgcc caccgctcat ccctcacctt ctccaaga                   408


<210>  79
<211>  145
<212>  PRT
<213>  Artificial sequence

<220>
<223>  PD1 -0-5 from (with out CYS93>Ser substitution)

<400>  79

Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp Asn Pro Pro Thr 
1               5                   10                  15      


Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp Asn Ala Thr Phe 
            20                  25                  30          


Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val Leu Asn Trp Tyr 
        35                  40                  45              


Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala Ala Phe Pro Glu 
    50                  55                  60                  


Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg Val Thr Gln Leu 
65                  70                  75                  80  


Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg Ala Arg Arg Asn 
                85                  90                  95      


Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu Ala Pro Lys Ala 
            100                 105                 110         


Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val Thr Glu Arg Arg 
        115                 120                 125             


Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro Arg Pro Ala Gly 
    130                 135                 140                 


Gln 
145 


<210>  80
<211>  435
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA OF #79

<400>  80
cccggctggt ttctggactc tccagacaga ccttggaacc ctccaacctt ctctcccgct       60

ctgctggtgg ttaccgaggg cgacaatgcc accttcacct gttccttcag caacacctcc      120

gagtccttcg tgctgaactg gtacagaatg tcccctagca accagaccga caagctggcc      180

gcctttcctg aggacagatc tcagccaggc caggactgtc ggttcagagt tacccagctg      240

cctaacggcc gggacttcca catgtctgtt gtgcgggcca gacggaacga ctctggcaca      300

tatctgtgcg gcgccatctc tctggctccc aaggctcaga tcaaagagtc tctgcgggcc      360

gagctgagag tgacagaaag acgagctgag gtgcccaccg ctcatccctc accttctcca      420

agacctgctg gccag                                                       435


<210>  81
<211>  143
<212>  PRT
<213>  Artificial sequence

<220>
<223>  PD1 -0-7 from (with out CYS93>Ser substitution) 143 aa

<400>  81

Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp Asn Pro Pro Thr 
1               5                   10                  15      


Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp Asn Ala Thr Phe 
            20                  25                  30          


Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val Leu Asn Trp Tyr 
        35                  40                  45              


Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala Ala Phe Pro Glu 
    50                  55                  60                  


Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg Val Thr Gln Leu 
65                  70                  75                  80  


Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg Ala Arg Arg Asn 
                85                  90                  95      


Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu Ala Pro Lys Ala 
            100                 105                 110         


Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val Thr Glu Arg Arg 
        115                 120                 125             


Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro Arg Pro Ala 
    130                 135                 140             


<210>  82
<211>  429
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA OF #81

<400>  82
cccggctggt ttctggactc tccagacaga ccttggaacc ctccaacctt ctctcccgct       60

ctgctggtgg ttaccgaggg cgacaatgcc accttcacct gttccttcag caacacctcc      120

gagtccttcg tgctgaactg gtacagaatg tcccctagca accagaccga caagctggcc      180

gcctttcctg aggacagatc tcagccaggc caggactgtc ggttcagagt tacccagctg      240

cctaacggcc gggacttcca catgtctgtt gtgcgggcca gacggaacga ctctggcaca      300

tatctgtgcg gcgccatctc tctggctccc aaggctcaga tcaaagagtc tctgcgggcc      360

gagctgagag tgacagaaag acgagctgag gtgcccaccg ctcatccctc accttctcca      420

agacctgct                                                              429


<210>  83
<211>  504
<212>  PRT
<213>  Artificial sequence

<220>
<223>  aa sequence of full length SIRP alpha

<400>  83

Met Glu Pro Ala Gly Pro Ala Pro Gly Arg Leu Gly Pro Leu Leu Cys 
1               5                   10                  15      


Leu Leu Leu Ala Ala Ser Cys Ala Trp Ser Gly Val Ala Gly Glu Glu 
            20                  25                  30          


Glu Leu Gln Val Ile Gln Pro Asp Lys Ser Val Leu Val Ala Ala Gly 
        35                  40                  45              


Glu Thr Ala Thr Leu Arg Cys Thr Ala Thr Ser Leu Ile Pro Val Gly 
    50                  55                  60                  


Pro Ile Gln Trp Phe Arg Gly Ala Gly Pro Gly Arg Glu Leu Ile Tyr 
65                  70                  75                  80  


Asn Gln Lys Glu Gly His Phe Pro Arg Val Thr Thr Val Ser Asp Leu 
                85                  90                  95      


Thr Lys Arg Asn Asn Met Asp Phe Ser Ile Arg Ile Gly Asn Ile Thr 
            100                 105                 110         


Pro Ala Asp Ala Gly Thr Tyr Tyr Cys Val Lys Phe Arg Lys Gly Ser 
        115                 120                 125             


Pro Asp Asp Val Glu Phe Lys Ser Gly Ala Gly Thr Glu Leu Ser Val 
    130                 135                 140                 


Arg Ala Lys Pro Ser Ala Pro Val Val Ser Gly Pro Ala Ala Arg Ala 
145                 150                 155                 160 


Thr Pro Gln His Thr Val Ser Phe Thr Cys Glu Ser His Gly Phe Ser 
                165                 170                 175     


Pro Arg Asp Ile Thr Leu Lys Trp Phe Lys Asn Gly Asn Glu Leu Ser 
            180                 185                 190         


Asp Phe Gln Thr Asn Val Asp Pro Val Gly Glu Ser Val Ser Tyr Ser 
        195                 200                 205             


Ile His Ser Thr Ala Lys Val Val Leu Thr Arg Glu Asp Val His Ser 
    210                 215                 220                 


Gln Val Ile Cys Glu Val Ala His Val Thr Leu Gln Gly Asp Pro Leu 
225                 230                 235                 240 


Arg Gly Thr Ala Asn Leu Ser Glu Thr Ile Arg Val Pro Pro Thr Leu 
                245                 250                 255     


Glu Val Thr Gln Gln Pro Val Arg Ala Glu Asn Gln Val Asn Val Thr 
            260                 265                 270         


Cys Gln Val Arg Lys Phe Tyr Pro Gln Arg Leu Gln Leu Thr Trp Leu 
        275                 280                 285             


Glu Asn Gly Asn Val Ser Arg Thr Glu Thr Ala Ser Thr Val Thr Glu 
    290                 295                 300                 


Asn Lys Asp Gly Thr Tyr Asn Trp Met Ser Trp Leu Leu Val Asn Val 
305                 310                 315                 320 


Ser Ala His Arg Asp Asp Val Lys Leu Thr Cys Gln Val Glu His Asp 
                325                 330                 335     


Gly Gln Pro Ala Val Ser Lys Ser His Asp Leu Lys Val Ser Ala His 
            340                 345                 350         


Pro Lys Glu Gln Gly Ser Asn Thr Ala Ala Glu Asn Thr Gly Ser Asn 
        355                 360                 365             


Glu Arg Asn Ile Tyr Ile Val Val Gly Val Val Cys Thr Leu Leu Val 
    370                 375                 380                 


Ala Leu Leu Met Ala Ala Leu Tyr Leu Val Arg Ile Arg Gln Lys Lys 
385                 390                 395                 400 


Ala Gln Gly Ser Thr Ser Ser Thr Arg Leu His Glu Pro Glu Lys Asn 
                405                 410                 415     


Ala Arg Glu Ile Thr Gln Asp Thr Asn Asp Ile Thr Tyr Ala Asp Leu 
            420                 425                 430         


Asn Leu Pro Lys Gly Lys Lys Pro Ala Pro Gln Ala Ala Glu Pro Asn 
        435                 440                 445             


Asn His Thr Glu Tyr Ala Ser Ile Gln Thr Ser Pro Gln Pro Ala Ser 
    450                 455                 460                 


Glu Asp Thr Leu Thr Tyr Ala Asp Leu Asp Met Val His Leu Asn Arg 
465                 470                 475                 480 


Thr Pro Lys Gln Pro Ala Pro Lys Pro Glu Pro Ser Phe Ser Glu Tyr 
                485                 490                 495     


Ala Ser Val Gln Val Pro Arg Lys 
            500                 


<210>  84
<211>  1512
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NA OF #83

<400>  84
atggagcccg ccggcccggc ccccggccgc ctcgggccgc tgctctgcct gctgctcgcc       60

gcgtcctgcg cctggtcagg agtggcgggt gaggaggagc tgcaggtgat tcagcctgac      120

aagtccgtat cagttgcagc tggagagtcg gccattctgc actgcactgt gacctccctg      180

atccctgtgg ggcccatcca gtggttcaga ggagctggac cagcccggga attaatctac      240

aatcaaaaag aaggccactt cccccgggta acaactgttt cagagtccac aaagagagaa      300

aacatggact tttccatcag catcagtaac atcaccccag cagatgccgg cacctactac      360

tgtgtgaagt tccggaaagg gagccctgac acggagttta agtctggagc aggcactgag      420

ctgtctgtgc gtgccaaacc ctctgccccc gtggtatcgg gccctgcggc gagggccaca      480

cctcagcaca cagtgagctt cacctgcgag tcccacggct tctcacccag agacatcacc      540

ctgaaatggt tcaaaaatgg gaatgagctc tcagacttcc agaccaacgt ggaccccgta      600

ggagagagcg tgtcctacag catccacagc acagccaagg tggtgctgac ccgcgaggac      660

gttcactctc aagtcatctg cgaggtggcc cacgtcacct tgcaggggga ccctcttcgt      720

gggactgcca acttgtctga gaccatccga gttccaccca ccttggaggt tactcaacag      780

cccgtgaggg cagagaacca ggtgaatgtc acctgccagg tgaggaagtt ctacccccag      840

agactacagc tgacctggtt ggagaatgga aacgtgtccc ggacagaaac ggcctcaacc      900

gttacagaga acaaggatgg tacctacaac tggatgagct ggctcctggt gaatgtatct      960

gcccacaggg atgatgtgaa gctcacctgc caggtggagc atgacgggca gccagcggtc     1020

agcaaaagcc atgacctgaa ggtctcagcc cacccgaagg agcagggctc aaataccgcc     1080

gctgagaaca ctggatctaa tgaacggaac atctatattg tggtgggtgt ggtgtgcacc     1140

ttgctggtgg ccctactgat ggcggccctc tacctcgtcc gaatcagaca gaagaaagcc     1200

cagggctcca cttcttctac aaggttgcat gagcccgaga agaatgccag agaaataaca     1260

caggacacaa atgatatcac atatgcagac ctgaacctgc ccaaggggaa gaagcctgct     1320

ccccaggctg cggagcccaa caaccacacg gagtatgcca gcattcagac cagcccgcag     1380

cccgcgtcgg aggacaccct cacctatgct gacctggaca tggtccacct caaccggacc     1440

cccaagcagc cggcccccaa gcctgagccg tccttctcag agtacgccag cgtccaggtc     1500

ccgaggaagt ga                                                         1512


<210>  85
<211>  343
<212>  PRT
<213>  Artificial sequence

<220>
<223>  ORIGINAL SIRPa DOMAIN (343AA)

<400>  85

Glu Glu Glu Leu Gln Val Ile Gln Pro Asp Lys Ser Val Leu Val Ala 
1               5                   10                  15      


Ala Gly Glu Thr Ala Thr Leu Arg Cys Thr Ala Thr Ser Leu Ile Pro 
            20                  25                  30          


Val Gly Pro Ile Gln Trp Phe Arg Gly Ala Gly Pro Gly Arg Glu Leu 
        35                  40                  45              


Ile Tyr Asn Gln Lys Glu Gly His Phe Pro Arg Val Thr Thr Val Ser 
    50                  55                  60                  


Asp Leu Thr Lys Arg Asn Asn Met Asp Phe Ser Ile Arg Ile Gly Asn 
65                  70                  75                  80  


Ile Thr Pro Ala Asp Ala Gly Thr Tyr Tyr Cys Val Lys Phe Arg Lys 
                85                  90                  95      


Gly Ser Pro Asp Asp Val Glu Phe Lys Ser Gly Ala Gly Thr Glu Leu 
            100                 105                 110         


Ser Val Arg Ala Lys Pro Ser Ala Pro Val Val Ser Gly Pro Ala Ala 
        115                 120                 125             


Arg Ala Thr Pro Gln His Thr Val Ser Phe Thr Cys Glu Ser His Gly 
    130                 135                 140                 


Phe Ser Pro Arg Asp Ile Thr Leu Lys Trp Phe Lys Asn Gly Asn Glu 
145                 150                 155                 160 


Leu Ser Asp Phe Gln Thr Asn Val Asp Pro Val Gly Glu Ser Val Ser 
                165                 170                 175     


Tyr Ser Ile His Ser Thr Ala Lys Val Val Leu Thr Arg Glu Asp Val 
            180                 185                 190         


His Ser Gln Val Ile Cys Glu Val Ala His Val Thr Leu Gln Gly Asp 
        195                 200                 205             


Pro Leu Arg Gly Thr Ala Asn Leu Ser Glu Thr Ile Arg Val Pro Pro 
    210                 215                 220                 


Thr Leu Glu Val Thr Gln Gln Pro Val Arg Ala Glu Asn Gln Val Asn 
225                 230                 235                 240 


Val Thr Cys Gln Val Arg Lys Phe Tyr Pro Gln Arg Leu Gln Leu Thr 
                245                 250                 255     


Trp Leu Glu Asn Gly Asn Val Ser Arg Thr Glu Thr Ala Ser Thr Val 
            260                 265                 270         


Thr Glu Asn Lys Asp Gly Thr Tyr Asn Trp Met Ser Trp Leu Leu Val 
        275                 280                 285             


Asn Val Ser Ala His Arg Asp Asp Val Lys Leu Thr Cys Gln Val Glu 
    290                 295                 300                 


His Asp Gly Gln Pro Ala Val Ser Lys Ser His Asp Leu Lys Val Ser 
305                 310                 315                 320 


Ala His Pro Lys Glu Gln Gly Ser Asn Thr Ala Ala Glu Asn Thr Gly 
                325                 330                 335     


Ser Asn Glu Arg Asn Ile Tyr 
            340             


<210>  86
<211>  1029
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NA OF #85

<400>  86
gaggaggagc tgcaggtgat tcagcctgac aagtccgtat cagttgcagc tggagagtcg       60

gccattctgc actgcactgt gacctccctg atccctgtgg ggcccatcca gtggttcaga      120

ggagctggac cagcccggga attaatctac aatcaaaaag aaggccactt cccccgggta      180

acaactgttt cagagtccac aaagagagaa aacatggact tttccatcag catcagtaac      240

atcaccccag cagatgccgg cacctactac tgtgtgaagt tccggaaagg gagccctgac      300

acggagttta agtctggagc aggcactgag ctgtctgtgc gtgccaaacc ctctgccccc      360

gtggtatcgg gccctgcggc gagggccaca cctcagcaca cagtgagctt cacctgcgag      420

tcccacggct tctcacccag agacatcacc ctgaaatggt tcaaaaatgg gaatgagctc      480

tcagacttcc agaccaacgt ggaccccgta ggagagagcg tgtcctacag catccacagc      540

acagccaagg tggtgctgac ccgcgaggac gttcactctc aagtcatctg cgaggtggcc      600

cacgtcacct tgcaggggga ccctcttcgt gggactgcca acttgtctga gaccatccga      660

gttccaccca ccttggaggt tactcaacag cccgtgaggg cagagaacca ggtgaatgtc      720

acctgccagg tgaggaagtt ctacccccag agactacagc tgacctggtt ggagaatgga      780

aacgtgtccc ggacagaaac ggcctcaacc gttacagaga acaaggatgg tacctacaac      840

tggatgagct ggctcctggt gaatgtatct gcccacaggg atgatgtgaa gctcacctgc      900

caggtggagc atgacgggca gccagcggtc agcaaaagcc atgacctgaa ggtctcagcc      960

cacccgaagg agcagggctc aaataccgcc gctgagaaca ctggatctaa tgaacggaac     1020

atctatatt                                                             1029


<210>  87
<211>  116
<212>  PRT
<213>  Artificial sequence

<220>
<223>  116 aa SIRPa segment

<400>  87

Glu Glu Glu Leu Gln Val Ile Gln Pro Asp Lys Ser Val Leu Val Ala 
1               5                   10                  15      


Ala Gly Glu Thr Ala Thr Leu Arg Cys Thr Ala Thr Ser Leu Ile Pro 
            20                  25                  30          


Val Gly Pro Ile Gln Trp Phe Arg Gly Ala Gly Pro Gly Arg Glu Leu 
        35                  40                  45              


Ile Tyr Asn Gln Lys Glu Gly His Phe Pro Arg Val Thr Thr Val Ser 
    50                  55                  60                  


Asp Leu Thr Lys Arg Asn Asn Met Asp Phe Ser Ile Arg Ile Gly Asn 
65                  70                  75                  80  


Ile Thr Pro Ala Asp Ala Gly Thr Tyr Tyr Cys Val Lys Phe Arg Lys 
                85                  90                  95      


Gly Ser Pro Asp Asp Val Glu Phe Lys Ser Gly Ala Gly Thr Glu Leu 
            100                 105                 110         


Ser Val Arg Ala 
        115     


<210>  88
<211>  348
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA OF #87

<400>  88
gaagaggaac tgcaagtgat ccagcctgac aagtccgtgc tggtggctgc tggcgaaacc       60

gccacactga gatgtaccgc cacctctctg atccctgtgg gccctatcca gtggtttaga      120

ggcgctggac ctggcagaga gctgatctac aaccagaaag agggccactt tcctagagtg      180

accaccgtgt ccgacctgac caagcggaac aacatggact tctccatccg gatcggcaac      240

atcacccctg ctgatgccgg cacctactac tgcgtgaagt tccggaaggg ctcccctgac      300

gacgtcgagt ttaaatccgg cgctggcacc gaactgtccg tgcgagct                   348


<210>  89
<211>  343
<212>  PRT
<213>  Artificial sequence

<220>
<223>  343 amino acids sequence of SIRPa with 4 point mutations

<400>  89

Glu Glu Glu Ile Gln Val Ile Gln Pro Asp Lys Ser Val Leu Val Ala 
1               5                   10                  15      


Ala Gly Glu Thr Ala Thr Leu Arg Cys Thr Ile Thr Ser Leu Ile Pro 
            20                  25                  30          


Val Gly Pro Ile Gln Trp Phe Arg Gly Ala Gly Pro Gly Arg Val Leu 
        35                  40                  45              


Ile Tyr Asn Gln Lys Glu Gly His Phe Pro Arg Val Thr Thr Val Ser 
    50                  55                  60                  


Asp Leu Thr Lys Arg Asn Asn Met Asp Phe Ser Ile Arg Ile Gly Asn 
65                  70                  75                  80  


Ile Thr Pro Ala Asp Ala Gly Thr Tyr Tyr Cys Ile Lys Phe Arg Lys 
                85                  90                  95      


Gly Ser Pro Asp Asp Val Glu Phe Lys Ser Gly Ala Gly Thr Glu Leu 
            100                 105                 110         


Ser Val Arg Ala Lys Pro Ser Ala Pro Val Val Ser Gly Pro Ala Ala 
        115                 120                 125             


Arg Ala Thr Pro Gln His Thr Val Ser Phe Thr Cys Glu Ser His Gly 
    130                 135                 140                 


Phe Ser Pro Arg Asp Ile Thr Leu Lys Trp Phe Lys Asn Gly Asn Glu 
145                 150                 155                 160 


Leu Ser Asp Phe Gln Thr Asn Val Asp Pro Val Gly Glu Ser Val Ser 
                165                 170                 175     


Tyr Ser Ile His Ser Thr Ala Lys Val Val Leu Thr Arg Glu Asp Val 
            180                 185                 190         


His Ser Gln Val Ile Cys Glu Val Ala His Val Thr Leu Gln Gly Asp 
        195                 200                 205             


Pro Leu Arg Gly Thr Ala Asn Leu Ser Glu Thr Ile Arg Val Pro Pro 
    210                 215                 220                 


Thr Leu Glu Val Thr Gln Gln Pro Val Arg Ala Glu Asn Gln Val Asn 
225                 230                 235                 240 


Val Thr Cys Gln Val Arg Lys Phe Tyr Pro Gln Arg Leu Gln Leu Thr 
                245                 250                 255     


Trp Leu Glu Asn Gly Asn Val Ser Arg Thr Glu Thr Ala Ser Thr Val 
            260                 265                 270         


Thr Glu Asn Lys Asp Gly Thr Tyr Asn Trp Met Ser Trp Leu Leu Val 
        275                 280                 285             


Asn Val Ser Ala His Arg Asp Asp Val Lys Leu Thr Cys Gln Val Glu 
    290                 295                 300                 


His Asp Gly Gln Pro Ala Val Ser Lys Ser His Asp Leu Lys Val Ser 
305                 310                 315                 320 


Ala His Pro Lys Glu Gln Gly Ser Asn Thr Ala Ala Glu Asn Thr Gly 
                325                 330                 335     


Ser Asn Glu Arg Asn Ile Tyr 
            340             


<210>  90
<211>  1029
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA OF #89

<400>  90
gaagaggaaa tccaagtgat ccagcctgac aagtccgtgc tggtggctgc tggcgaaacc       60

gccacactga gatgtaccat cacctctctg atccctgtgg gccctatcca gtggtttaga      120

ggcgctggac ctggcagagt gctgatctac aaccagaaag agggccactt tcctagagtg      180

accaccgtgt ccgacctgac caagcggaac aacatggact tctccatccg gatcggcaac      240

atcacccctg ctgatgccgg cacctactac tgcatcaagt tccggaaggg ctcccctgac      300

gacgtcgagt ttaaatccgg cgctggcacc gaactgtccg tgcgagctaa accttctgct      360

cccgtggtgt ctggccctgc cgctagagct acacctcagc acaccgtgtc ttttacctgc      420

gagtcccacg gcttcagccc tagagacatc accctgaagt ggttcaagaa cggcaacgag      480

ctgtccgact tccagaccaa cgtggaccct gtgggagagt ccgtgtccta ctccatccac      540

tctaccgcca aggtggtgct gacccgagag gacgtgcaca gccaagtgat ctgtgaagtg      600

gcccacgtga ccctccaggg cgatcctttg agaggcaccg ccaacctgtc cgagacaatc      660

agagtgcctc ctacactgga agtgacccag cagcctgtgc gggccgagaa tcaagtgaac      720

gtgacctgcc aagtgcggaa gttctaccct cagagactgc agctgacctg gctggaaaac      780

ggcaatgtgt ccagaaccga gacagcctcc accgtgaccg agaacaagga tggcacctac      840

aattggatgt cctggctgct cgtgaacgtg tccgctcaca gagatgacgt gaagctgaca      900

tgccaggtgg aacacgatgg ccagcctgcc gtgtctaagt cccacgacct gaaagtgtct      960

gctcacccca aagagcaggg ctccaatacc gccgctgaga acaccggctc caacgagaga     1020

aacatctac                                                             1029


<210>  91
<211>  116
<212>  PRT
<213>  Artificial sequence

<220>
<223>  116 amino acids sequence of SIRPa with 4 point mutations

<400>  91

Glu Glu Glu Ile Gln Val Ile Gln Pro Asp Lys Ser Val Leu Val Ala 
1               5                   10                  15      


Ala Gly Glu Thr Ala Thr Leu Arg Cys Thr Ile Thr Ser Leu Ile Pro 
            20                  25                  30          


Val Gly Pro Ile Gln Trp Phe Arg Gly Ala Gly Pro Gly Arg Val Leu 
        35                  40                  45              


Ile Tyr Asn Gln Lys Glu Gly His Phe Pro Arg Val Thr Thr Val Ser 
    50                  55                  60                  


Asp Leu Thr Lys Arg Asn Asn Met Asp Phe Ser Ile Arg Ile Gly Asn 
65                  70                  75                  80  


Ile Thr Pro Ala Asp Ala Gly Thr Tyr Tyr Cys Ile Lys Phe Arg Lys 
                85                  90                  95      


Gly Ser Pro Asp Asp Val Glu Phe Lys Ser Gly Ala Gly Thr Glu Leu 
            100                 105                 110         


Ser Val Arg Ala 
        115     


<210>  92
<211>  348
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA OF #91

<400>  92
gaagaggaaa tccaagtgat ccagcctgac aagtccgtgc tggtggctgc tggcgaaacc       60

gccacactga gatgtaccat cacctctctg atccctgtgg gccctatcca gtggtttaga      120

ggcgctggac ctggcagagt gctgatctac aaccagaaag agggccactt tcctagagtg      180

accaccgtgt ccgacctgac caagcggaac aacatggact tctccatccg gatcggcaac      240

atcacccctg ctgatgccgg cacctactac tgcatcaagt tccggaaggg ctcccctgac      300

gacgtcgagt ttaaatccgg cgctggcacc gaactgtccg tgcgagct                   348


<210>  93
<211>  117
<212>  PRT
<213>  Artificial sequence

<220>
<223>  aa SIRPa segment - addition K at the C-ter of SEQ ID 87  (117 aa 
       SIRPa segment)

<400>  93

Glu Glu Glu Leu Gln Val Ile Gln Pro Asp Lys Ser Val Leu Val Ala 
1               5                   10                  15      


Ala Gly Glu Thr Ala Thr Leu Arg Cys Thr Ala Thr Ser Leu Ile Pro 
            20                  25                  30          


Val Gly Pro Ile Gln Trp Phe Arg Gly Ala Gly Pro Gly Arg Glu Leu 
        35                  40                  45              


Ile Tyr Asn Gln Lys Glu Gly His Phe Pro Arg Val Thr Thr Val Ser 
    50                  55                  60                  


Asp Leu Thr Lys Arg Asn Asn Met Asp Phe Ser Ile Arg Ile Gly Asn 
65                  70                  75                  80  


Ile Thr Pro Ala Asp Ala Gly Thr Tyr Tyr Cys Val Lys Phe Arg Lys 
                85                  90                  95      


Gly Ser Pro Asp Asp Val Glu Phe Lys Ser Gly Ala Gly Thr Glu Leu 
            100                 105                 110         


Ser Val Arg Ala Lys 
        115         


<210>  94
<211>  351
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA OF #93

<400>  94
gaagaggaac tgcaagtgat ccagcctgac aagtccgtgc tggtggctgc tggcgaaacc       60

gccacactga gatgtaccgc cacctctctg atccctgtgg gccctatcca gtggtttaga      120

ggcgctggac ctggcagaga gctgatctac aaccagaaag agggccactt tcctagagtg      180

accaccgtgt ccgacctgac caagcggaac aacatggact tctccatccg gatcggcaac      240

atcacccctg ctgatgccgg cacctactac tgcgtgaagt tccggaaggg ctcccctgac      300

gacgtcgagt ttaaatccgg cgctggcacc gaactgtccg tgcgagctaa g               351


<210>  95
<211>  440
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LILRB2 Full ECD AA SEQUENCE

<400>  95

Gln Thr Gly Thr Ile Pro Lys Pro Thr Leu Trp Ala Glu Pro Asp Ser 
1               5                   10                  15      


Val Ile Thr Gln Gly Ser Pro Val Thr Leu Ser Cys Gln Gly Ser Leu 
            20                  25                  30          


Glu Ala Gln Glu Tyr Arg Leu Tyr Arg Glu Lys Lys Ser Ala Ser Trp 
        35                  40                  45              


Ile Thr Arg Ile Arg Pro Glu Leu Val Lys Asn Gly Gln Phe His Ile 
    50                  55                  60                  


Pro Ser Ile Thr Trp Glu His Thr Gly Arg Tyr Gly Cys Gln Tyr Tyr 
65                  70                  75                  80  


Ser Arg Ala Arg Trp Ser Glu Leu Ser Asp Pro Leu Val Leu Val Met 
                85                  90                  95      


Thr Gly Ala Tyr Pro Lys Pro Thr Leu Ser Ala Gln Pro Ser Pro Val 
            100                 105                 110         


Val Thr Ser Gly Gly Arg Val Thr Leu Gln Cys Glu Ser Gln Val Ala 
        115                 120                 125             


Phe Gly Gly Phe Ile Leu Cys Lys Glu Gly Glu Glu Glu His Pro Gln 
    130                 135                 140                 


Cys Leu Asn Ser Gln Pro His Ala Arg Gly Ser Ser Arg Ala Ile Phe 
145                 150                 155                 160 


Ser Val Gly Pro Val Ser Pro Asn Arg Arg Trp Ser His Arg Cys Tyr 
                165                 170                 175     


Gly Tyr Asp Leu Asn Ser Pro Tyr Val Trp Ser Ser Pro Ser Asp Leu 
            180                 185                 190         


Leu Glu Leu Leu Val Pro Gly Val Ser Lys Lys Pro Ser Leu Ser Val 
        195                 200                 205             


Gln Pro Gly Pro Val Val Ala Pro Gly Glu Ser Leu Thr Leu Gln Cys 
    210                 215                 220                 


Val Ser Asp Val Gly Tyr Asp Arg Phe Val Leu Tyr Lys Glu Gly Glu 
225                 230                 235                 240 


Arg Asp Leu Arg Gln Leu Pro Gly Arg Gln Pro Gln Ala Gly Leu Ser 
                245                 250                 255     


Gln Ala Asn Phe Thr Leu Gly Pro Val Ser Arg Ser Tyr Gly Gly Gln 
            260                 265                 270         


Tyr Arg Cys Tyr Gly Ala His Asn Leu Ser Ser Glu Cys Ser Ala Pro 
        275                 280                 285             


Ser Asp Pro Leu Asp Ile Leu Ile Thr Gly Gln Ile Arg Gly Thr Pro 
    290                 295                 300                 


Phe Ile Ser Val Gln Pro Gly Pro Thr Val Ala Ser Gly Glu Asn Val 
305                 310                 315                 320 


Thr Leu Leu Cys Gln Ser Trp Arg Gln Phe His Thr Phe Leu Leu Thr 
                325                 330                 335     


Lys Ala Gly Ala Ala Asp Ala Pro Leu Arg Leu Arg Ser Ile His Glu 
            340                 345                 350         


Tyr Pro Lys Tyr Gln Ala Glu Phe Pro Met Ser Pro Val Thr Ser Ala 
        355                 360                 365             


His Ala Gly Thr Tyr Arg Cys Tyr Gly Ser Leu Asn Ser Asp Pro Tyr 
    370                 375                 380                 


Leu Leu Ser His Pro Ser Glu Pro Leu Glu Leu Val Val Ser Gly Pro 
385                 390                 395                 400 


Ser Met Gly Ser Ser Pro Pro Pro Thr Gly Pro Ile Ser Thr Pro Ala 
                405                 410                 415     


Gly Pro Glu Asp Gln Pro Leu Thr Pro Thr Gly Ser Asp Pro Gln Ser 
            420                 425                 430         


Gly Leu Gly Arg His Leu Gly Val 
        435                 440 


<210>  96
<211>  398
<212>  PRT
<213>  Artificial sequence

<220>
<223>  LILRB2 D1-4, AA 22-419 (Q8N423-1 UniParc) AA SEQUENCE

<400>  96

Gln Thr Gly Thr Ile Pro Lys Pro Thr Leu Trp Ala Glu Pro Asp Ser 
1               5                   10                  15      


Val Ile Thr Gln Gly Ser Pro Val Thr Leu Ser Cys Gln Gly Ser Leu 
            20                  25                  30          


Glu Ala Gln Glu Tyr Arg Leu Tyr Arg Glu Lys Lys Ser Ala Ser Trp 
        35                  40                  45              


Ile Thr Arg Ile Arg Pro Glu Leu Val Lys Asn Gly Gln Phe His Ile 
    50                  55                  60                  


Pro Ser Ile Thr Trp Glu His Thr Gly Arg Tyr Gly Cys Gln Tyr Tyr 
65                  70                  75                  80  


Ser Arg Ala Arg Trp Ser Glu Leu Ser Asp Pro Leu Val Leu Val Met 
                85                  90                  95      


Thr Gly Ala Tyr Pro Lys Pro Thr Leu Ser Ala Gln Pro Ser Pro Val 
            100                 105                 110         


Val Thr Ser Gly Gly Arg Val Thr Leu Gln Cys Glu Ser Gln Val Ala 
        115                 120                 125             


Phe Gly Gly Phe Ile Leu Cys Lys Glu Gly Glu Glu Glu His Pro Gln 
    130                 135                 140                 


Cys Leu Asn Ser Gln Pro His Ala Arg Gly Ser Ser Arg Ala Ile Phe 
145                 150                 155                 160 


Ser Val Gly Pro Val Ser Pro Asn Arg Arg Trp Ser His Arg Cys Tyr 
                165                 170                 175     


Gly Tyr Asp Leu Asn Ser Pro Tyr Val Trp Ser Ser Pro Ser Asp Leu 
            180                 185                 190         


Leu Glu Leu Leu Val Pro Gly Val Ser Lys Lys Pro Ser Leu Ser Val 
        195                 200                 205             


Gln Pro Gly Pro Val Val Ala Pro Gly Glu Ser Leu Thr Leu Gln Cys 
    210                 215                 220                 


Val Ser Asp Val Gly Tyr Asp Arg Phe Val Leu Tyr Lys Glu Gly Glu 
225                 230                 235                 240 


Arg Asp Leu Arg Gln Leu Pro Gly Arg Gln Pro Gln Ala Gly Leu Ser 
                245                 250                 255     


Gln Ala Asn Phe Thr Leu Gly Pro Val Ser Arg Ser Tyr Gly Gly Gln 
            260                 265                 270         


Tyr Arg Cys Tyr Gly Ala His Asn Leu Ser Ser Glu Cys Ser Ala Pro 
        275                 280                 285             


Ser Asp Pro Leu Asp Ile Leu Ile Thr Gly Gln Ile Arg Gly Thr Pro 
    290                 295                 300                 


Phe Ile Ser Val Gln Pro Gly Pro Thr Val Ala Ser Gly Glu Asn Val 
305                 310                 315                 320 


Thr Leu Leu Cys Gln Ser Trp Arg Gln Phe His Thr Phe Leu Leu Thr 
                325                 330                 335     


Lys Ala Gly Ala Ala Asp Ala Pro Leu Arg Leu Arg Ser Ile His Glu 
            340                 345                 350         


Tyr Pro Lys Tyr Gln Ala Glu Phe Pro Met Ser Pro Val Thr Ser Ala 
        355                 360                 365             


His Ala Gly Thr Tyr Arg Cys Tyr Gly Ser Leu Asn Ser Asp Pro Tyr 
    370                 375                 380                 


Leu Leu Ser His Pro Ser Glu Pro Leu Glu Leu Val Val Ser 
385                 390                 395             


<210>  97
<211>  1194
<212>  DNA
<213>  Artificial sequence

<220>
<223>  LILRB2 D1-4, AA 22-419 (Q8N423-1 UniParc) NA SEQUENCE

<400>  97
cagaccggca caatccccaa gcctaccctg tgggccgagc cagatagcgt gatcacccag       60

ggctcccccg tgacactgtc ttgccagggc agcctggagg cacaggagta ccggctgtat      120

agagagaaga agagcgcctc ctggatcacc cggatcagac ccgagctggt gaagaacggc      180

cagtttcaca tcccttccat cacctgggag cacacaggcc ggtacggatg ccagtactat      240

tctcgggcca gatggagcga gctgtccgac cccctggtgc tggtcatgac cggcgcctat      300

ccaaagccca cactgtccgc ccagccttct ccagtggtga cctctggcgg cagagtgaca      360

ctgcagtgtg agagccaggt ggccttcggc ggctttatcc tgtgcaagga gggcgaggag      420

gagcaccccc agtgtctgaa tagccagcct cacgcccggg gcagctccag agccatcttc      480

tctgtgggcc ctgtgagccc aaaccggaga tggtcccaca ggtgctacgg ctatgacctg      540

aacagcccat acgtgtggtc tagcccctct gatctgctgg agctgctggt gcctggcgtg      600

agcaagaagc catctctgag cgtgcagcca ggccctgtgg tggcacctgg cgagtctctg      660

accctgcagt gcgtgagcga cgtgggctac gatcggttcg tgctgtataa ggagggagag      720

agggatctga ggcagctgcc aggcagacag cctcaggcag gactgtccca ggcaaacttt      780

acactgggcc ccgtgagccg gagctacggc ggacagtacc gctgctatgg agcacacaat      840

ctgtcctctg agtgttctgc ccccagcgac cccctggaca tcctgatcac cggccagatc      900

aggggcacac cattcatcag cgtgcagcca ggaccaaccg tggcctccgg cgagaacgtg      960

acactgctgt gccagagctg gcgccagttc cacacctttc tgctgacaaa ggcaggagca     1020

gcagacgcac ctctgaggct gcgctccatc cacgagtacc caaagtatca ggccgagttt     1080

ccaatgagcc ccgtgacctc cgcccacgca ggcacataca gatgctatgg cagcctgaac     1140

agcgacccct acctgctgag ccacccttcc gagccactgg agctggtggt gtcc           1194


<210>  98
<211>  198
<212>  PRT
<213>  Artificial sequence

<220>
<223>  LILRB2 D1-2, AA 22-219 (Q8N423-1 UniParc) AA SEQUENCE

<400>  98

Gln Thr Gly Thr Ile Pro Lys Pro Thr Leu Trp Ala Glu Pro Asp Ser 
1               5                   10                  15      


Val Ile Thr Gln Gly Ser Pro Val Thr Leu Ser Cys Gln Gly Ser Leu 
            20                  25                  30          


Glu Ala Gln Glu Tyr Arg Leu Tyr Arg Glu Lys Lys Ser Ala Ser Trp 
        35                  40                  45              


Ile Thr Arg Ile Arg Pro Glu Leu Val Lys Asn Gly Gln Phe His Ile 
    50                  55                  60                  


Pro Ser Ile Thr Trp Glu His Thr Gly Arg Tyr Gly Cys Gln Tyr Tyr 
65                  70                  75                  80  


Ser Arg Ala Arg Trp Ser Glu Leu Ser Asp Pro Leu Val Leu Val Met 
                85                  90                  95      


Thr Gly Ala Tyr Pro Lys Pro Thr Leu Ser Ala Gln Pro Ser Pro Val 
            100                 105                 110         


Val Thr Ser Gly Gly Arg Val Thr Leu Gln Cys Glu Ser Gln Val Ala 
        115                 120                 125             


Phe Gly Gly Phe Ile Leu Cys Lys Glu Gly Glu Glu Glu His Pro Gln 
    130                 135                 140                 


Cys Leu Asn Ser Gln Pro His Ala Arg Gly Ser Ser Arg Ala Ile Phe 
145                 150                 155                 160 


Ser Val Gly Pro Val Ser Pro Asn Arg Arg Trp Ser His Arg Cys Tyr 
                165                 170                 175     


Gly Tyr Asp Leu Asn Ser Pro Tyr Val Trp Ser Ser Pro Ser Asp Leu 
            180                 185                 190         


Leu Glu Leu Leu Val Pro 
        195             


<210>  99
<211>  594
<212>  DNA
<213>  Artificial sequence

<220>
<223>  LILRB2 D1-2, AA 22-219 (Q8N423-1 UniParc) NA SEQUENCE

<400>  99
cagaccggca caatccccaa gcctaccctg tgggccgagc cagatagcgt gatcacccag       60

ggctcccccg tgacactgtc ttgccagggc agcctggagg cacaggagta ccggctgtat      120

agagagaaga agagcgcctc ctggatcacc cggatcagac ccgagctggt gaagaacggc      180

cagtttcaca tcccttccat cacctgggag cacacaggcc ggtacggatg ccagtactat      240

tctcgggcca gatggagcga gctgtccgac cccctggtgc tggtcatgac cggcgcctat      300

ccaaagccca cactgtccgc ccagccttct ccagtggtga cctctggcgg cagagtgaca      360

ctgcagtgtg agagccaggt ggccttcggc ggctttatcc tgtgcaagga gggcgaggag      420

gagcaccccc agtgtctgaa tagccagcct cacgcccggg gcagctccag agccatcttc      480

tctgtgggcc ctgtgagccc aaaccggaga tggtcccaca ggtgctacgg ctatgacctg      540

aacagcccat acgtgtggtc tagcccctct gatctgctgg agctgctggt gcct            594


<210>  100
<211>  697
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Siglec 10 full length (Q96LC7-1 Uniprot) AA SEQUENCE

<400>  100

Met Leu Leu Pro Leu Leu Leu Ser Ser Leu Leu Gly Gly Ser Gln Ala 
1               5                   10                  15      


Met Asp Gly Arg Phe Trp Ile Arg Val Gln Glu Ser Val Met Val Pro 
            20                  25                  30          


Glu Gly Leu Cys Ile Ser Val Pro Cys Ser Phe Ser Tyr Pro Arg Gln 
        35                  40                  45              


Asp Trp Thr Gly Ser Thr Pro Ala Tyr Gly Tyr Trp Phe Lys Ala Val 
    50                  55                  60                  


Thr Glu Thr Thr Lys Gly Ala Pro Val Ala Thr Asn His Gln Ser Arg 
65                  70                  75                  80  


Glu Val Glu Met Ser Thr Arg Gly Arg Phe Gln Leu Thr Gly Asp Pro 
                85                  90                  95      


Ala Lys Gly Asn Cys Ser Leu Val Ile Arg Asp Ala Gln Met Gln Asp 
            100                 105                 110         


Glu Ser Gln Tyr Phe Phe Arg Val Glu Arg Gly Ser Tyr Val Arg Tyr 
        115                 120                 125             


Asn Phe Met Asn Asp Gly Phe Phe Leu Lys Val Thr Ala Leu Thr Gln 
    130                 135                 140                 


Lys Pro Asp Val Tyr Ile Pro Glu Thr Leu Glu Pro Gly Gln Pro Val 
145                 150                 155                 160 


Thr Val Ile Cys Val Phe Asn Trp Ala Phe Glu Glu Cys Pro Pro Pro 
                165                 170                 175     


Ser Phe Ser Trp Thr Gly Ala Ala Leu Ser Ser Gln Gly Thr Lys Pro 
            180                 185                 190         


Thr Thr Ser His Phe Ser Val Leu Ser Phe Thr Pro Arg Pro Gln Asp 
        195                 200                 205             


His Asn Thr Asp Leu Thr Cys His Val Asp Phe Ser Arg Lys Gly Val 
    210                 215                 220                 


Ser Ala Gln Arg Thr Val Arg Leu Arg Val Ala Tyr Ala Pro Arg Asp 
225                 230                 235                 240 


Leu Val Ile Ser Ile Ser Arg Asp Asn Thr Pro Ala Leu Glu Pro Gln 
                245                 250                 255     


Pro Gln Gly Asn Val Pro Tyr Leu Glu Ala Gln Lys Gly Gln Phe Leu 
            260                 265                 270         


Arg Leu Leu Cys Ala Ala Asp Ser Gln Pro Pro Ala Thr Leu Ser Trp 
        275                 280                 285             


Val Leu Gln Asn Arg Val Leu Ser Ser Ser His Pro Trp Gly Pro Arg 
    290                 295                 300                 


Pro Leu Gly Leu Glu Leu Pro Gly Val Lys Ala Gly Asp Ser Gly Arg 
305                 310                 315                 320 


Tyr Thr Cys Arg Ala Glu Asn Arg Leu Gly Ser Gln Gln Arg Ala Leu 
                325                 330                 335     


Asp Leu Ser Val Gln Tyr Pro Pro Glu Asn Leu Arg Val Met Val Ser 
            340                 345                 350         


Gln Ala Asn Arg Thr Val Leu Glu Asn Leu Gly Asn Gly Thr Ser Leu 
        355                 360                 365             


Pro Val Leu Glu Gly Gln Ser Leu Cys Leu Val Cys Val Thr His Ser 
    370                 375                 380                 


Ser Pro Pro Ala Arg Leu Ser Trp Thr Gln Arg Gly Gln Val Leu Ser 
385                 390                 395                 400 


Pro Ser Gln Pro Ser Asp Pro Gly Val Leu Glu Leu Pro Arg Val Gln 
                405                 410                 415     


Val Glu His Glu Gly Glu Phe Thr Cys His Ala Arg His Pro Leu Gly 
            420                 425                 430         


Ser Gln His Val Ser Leu Ser Leu Ser Val His Tyr Ser Pro Lys Leu 
        435                 440                 445             


Leu Gly Pro Ser Cys Ser Trp Glu Ala Glu Gly Leu His Cys Ser Cys 
    450                 455                 460                 


Ser Ser Gln Ala Ser Pro Ala Pro Ser Leu Arg Trp Trp Leu Gly Glu 
465                 470                 475                 480 


Glu Leu Leu Glu Gly Asn Ser Ser Gln Asp Ser Phe Glu Val Thr Pro 
                485                 490                 495     


Ser Ser Ala Gly Pro Trp Ala Asn Ser Ser Leu Ser Leu His Gly Gly 
            500                 505                 510         


Leu Ser Ser Gly Leu Arg Leu Arg Cys Glu Ala Trp Asn Val His Gly 
        515                 520                 525             


Ala Gln Ser Gly Ser Ile Leu Gln Leu Pro Asp Lys Lys Gly Leu Ile 
    530                 535                 540                 


Ser Thr Ala Phe Ser Asn Gly Ala Phe Leu Gly Ile Gly Ile Thr Ala 
545                 550                 555                 560 


Leu Leu Phe Leu Cys Leu Ala Leu Ile Ile Met Lys Ile Leu Pro Lys 
                565                 570                 575     


Arg Arg Thr Gln Thr Glu Thr Pro Arg Pro Arg Phe Ser Arg His Ser 
            580                 585                 590         


Thr Ile Leu Asp Tyr Ile Asn Val Val Pro Thr Ala Gly Pro Leu Ala 
        595                 600                 605             


Gln Lys Arg Asn Gln Lys Ala Thr Pro Asn Ser Pro Arg Thr Pro Leu 
    610                 615                 620                 


Pro Pro Gly Ala Pro Ser Pro Glu Ser Lys Lys Asn Gln Lys Lys Gln 
625                 630                 635                 640 


Tyr Gln Leu Pro Ser Phe Pro Glu Pro Lys Ser Ser Thr Gln Ala Pro 
                645                 650                 655     


Glu Ser Gln Glu Ser Gln Glu Glu Leu His Tyr Ala Thr Leu Asn Phe 
            660                 665                 670         


Pro Gly Val Arg Pro Arg Pro Glu Ala Arg Met Pro Lys Gly Thr Gln 
        675                 680                 685             


Ala Asp Tyr Ala Glu Val Lys Phe Gln 
    690                 695         


<210>  101
<211>  128
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Siglec 10, AA 18-145 NO MUTATION (Q96LC7-1 Uniprot) AA SEQUENCE

<400>  101

Asp Gly Arg Phe Trp Ile Arg Val Gln Glu Ser Val Met Val Pro Glu 
1               5                   10                  15      


Gly Leu Cys Ile Ser Val Pro Cys Ser Phe Ser Tyr Pro Arg Gln Asp 
            20                  25                  30          


Trp Thr Gly Ser Thr Pro Ala Tyr Gly Tyr Trp Phe Lys Ala Val Thr 
        35                  40                  45              


Glu Thr Thr Lys Gly Ala Pro Val Ala Thr Asn His Gln Ser Arg Glu 
    50                  55                  60                  


Val Glu Met Ser Thr Arg Gly Arg Phe Gln Leu Thr Gly Asp Pro Ala 
65                  70                  75                  80  


Lys Gly Asn Cys Ser Leu Val Ile Arg Asp Ala Gln Met Gln Asp Glu 
                85                  90                  95      


Ser Gln Tyr Phe Phe Arg Val Glu Arg Gly Ser Tyr Val Arg Tyr Asn 
            100                 105                 110         


Phe Met Asn Asp Gly Phe Phe Leu Lys Val Thr Ala Leu Thr Gln Lys 
        115                 120                 125             


<210>  102
<211>  186
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Siglec 10, AA 18-145 NO MUTATION (Q96LC7-1 Uniprot) NA SEQUENCE

<400>  102
gatggccggt tttggatcag agtgcaggag tccgtgatgg tgcctgaggg cctgtgcatc       60

agcgtgccat gctccttctc ttaccccaga caggactgga ccggctctac acccgcctac      120

ggctattggt ttaaggccgt gaccgagaca acaaagggcg cccctgtggc cacaaaccac      180

cagagc                                                                 186


<210>  103
<211>  128
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Siglec 10, AA 18-145 plus C36S (Q96LC7-1 Uniprot) AA SEQUENCE

<400>  103

Asp Gly Arg Phe Trp Ile Arg Val Gln Glu Ser Val Met Val Pro Glu 
1               5                   10                  15      


Gly Leu Ser Ile Ser Val Pro Cys Ser Phe Ser Tyr Pro Arg Gln Asp 
            20                  25                  30          


Trp Thr Gly Ser Thr Pro Ala Tyr Gly Tyr Trp Phe Lys Ala Val Thr 
        35                  40                  45              


Glu Thr Thr Lys Gly Ala Pro Val Ala Thr Asn His Gln Ser Arg Glu 
    50                  55                  60                  


Val Glu Met Ser Thr Arg Gly Arg Phe Gln Leu Thr Gly Asp Pro Ala 
65                  70                  75                  80  


Lys Gly Asn Cys Ser Leu Val Ile Arg Asp Ala Gln Met Gln Asp Glu 
                85                  90                  95      


Ser Gln Tyr Phe Phe Arg Val Glu Arg Gly Ser Tyr Val Arg Tyr Asn 
            100                 105                 110         


Phe Met Asn Asp Gly Phe Phe Leu Lys Val Thr Ala Leu Thr Gln Lys 
        115                 120                 125             


<210>  104
<211>  384
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Siglec 10, AA 18-145 plus C36S (Q96LC7-1 Uniprot) NA SEQUENCE

<400>  104
gatggccggt tttggatcag agtgcaggag tccgtgatgg tgcctgaggg cctgtctatc       60

agcgtgccat gctccttctc ttaccccaga caggactgga ccggctctac acccgcctac      120

ggctattggt ttaaggccgt gaccgagaca acaaagggcg cccctgtggc cacaaaccac      180

cagagcagag aggtggagat gtccacccgg ggcagattcc agctgacagg cgaccccgcc      240

aagggcaatt gtagcctggt catcagggac gcccagatgc aggatgagtc tcagtacttc      300

tttagggtgg agcgcggcag ctacgtgcgc tataacttta tgaatgatgg cttctttctg      360

aaggtgaccg ccctgacaca gaag                                             384


<210>  105
<211>  534
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Siglec 10 full ECD, AA 17-550 (Q96LC7-1 Uniprot) AA SEQUENCE

<400>  105

Met Asp Gly Arg Phe Trp Ile Arg Val Gln Glu Ser Val Met Val Pro 
1               5                   10                  15      


Glu Gly Leu Cys Ile Ser Val Pro Cys Ser Phe Ser Tyr Pro Arg Gln 
            20                  25                  30          


Asp Trp Thr Gly Ser Thr Pro Ala Tyr Gly Tyr Trp Phe Lys Ala Val 
        35                  40                  45              


Thr Glu Thr Thr Lys Gly Ala Pro Val Ala Thr Asn His Gln Ser Arg 
    50                  55                  60                  


Glu Val Glu Met Ser Thr Arg Gly Arg Phe Gln Leu Thr Gly Asp Pro 
65                  70                  75                  80  


Ala Lys Gly Asn Cys Ser Leu Val Ile Arg Asp Ala Gln Met Gln Asp 
                85                  90                  95      


Glu Ser Gln Tyr Phe Phe Arg Val Glu Arg Gly Ser Tyr Val Arg Tyr 
            100                 105                 110         


Asn Phe Met Asn Asp Gly Phe Phe Leu Lys Val Thr Ala Leu Thr Gln 
        115                 120                 125             


Lys Pro Asp Val Tyr Ile Pro Glu Thr Leu Glu Pro Gly Gln Pro Val 
    130                 135                 140                 


Thr Val Ile Cys Val Phe Asn Trp Ala Phe Glu Glu Cys Pro Pro Pro 
145                 150                 155                 160 


Ser Phe Ser Trp Thr Gly Ala Ala Leu Ser Ser Gln Gly Thr Lys Pro 
                165                 170                 175     


Thr Thr Ser His Phe Ser Val Leu Ser Phe Thr Pro Arg Pro Gln Asp 
            180                 185                 190         


His Asn Thr Asp Leu Thr Cys His Val Asp Phe Ser Arg Lys Gly Val 
        195                 200                 205             


Ser Ala Gln Arg Thr Val Arg Leu Arg Val Ala Tyr Ala Pro Arg Asp 
    210                 215                 220                 


Leu Val Ile Ser Ile Ser Arg Asp Asn Thr Pro Ala Leu Glu Pro Gln 
225                 230                 235                 240 


Pro Gln Gly Asn Val Pro Tyr Leu Glu Ala Gln Lys Gly Gln Phe Leu 
                245                 250                 255     


Arg Leu Leu Cys Ala Ala Asp Ser Gln Pro Pro Ala Thr Leu Ser Trp 
            260                 265                 270         


Val Leu Gln Asn Arg Val Leu Ser Ser Ser His Pro Trp Gly Pro Arg 
        275                 280                 285             


Pro Leu Gly Leu Glu Leu Pro Gly Val Lys Ala Gly Asp Ser Gly Arg 
    290                 295                 300                 


Tyr Thr Cys Arg Ala Glu Asn Arg Leu Gly Ser Gln Gln Arg Ala Leu 
305                 310                 315                 320 


Asp Leu Ser Val Gln Tyr Pro Pro Glu Asn Leu Arg Val Met Val Ser 
                325                 330                 335     


Gln Ala Asn Arg Thr Val Leu Glu Asn Leu Gly Asn Gly Thr Ser Leu 
            340                 345                 350         


Pro Val Leu Glu Gly Gln Ser Leu Cys Leu Val Cys Val Thr His Ser 
        355                 360                 365             


Ser Pro Pro Ala Arg Leu Ser Trp Thr Gln Arg Gly Gln Val Leu Ser 
    370                 375                 380                 


Pro Ser Gln Pro Ser Asp Pro Gly Val Leu Glu Leu Pro Arg Val Gln 
385                 390                 395                 400 


Val Glu His Glu Gly Glu Phe Thr Cys His Ala Arg His Pro Leu Gly 
                405                 410                 415     


Ser Gln His Val Ser Leu Ser Leu Ser Val His Tyr Ser Pro Lys Leu 
            420                 425                 430         


Leu Gly Pro Ser Cys Ser Trp Glu Ala Glu Gly Leu His Cys Ser Cys 
        435                 440                 445             


Ser Ser Gln Ala Ser Pro Ala Pro Ser Leu Arg Trp Trp Leu Gly Glu 
    450                 455                 460                 


Glu Leu Leu Glu Gly Asn Ser Ser Gln Asp Ser Phe Glu Val Thr Pro 
465                 470                 475                 480 


Ser Ser Ala Gly Pro Trp Ala Asn Ser Ser Leu Ser Leu His Gly Gly 
                485                 490                 495     


Leu Ser Ser Gly Leu Arg Leu Arg Cys Glu Ala Trp Asn Val His Gly 
            500                 505                 510         


Ala Gln Ser Gly Ser Ile Leu Gln Leu Pro Asp Lys Lys Gly Leu Ile 
        515                 520                 525             


Ser Thr Ala Phe Ser Asn 
    530                 


<210>  106
<211>  244
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TIGIT full length (Q495A1 Uniprot ID) AA SEQUENCE

<400>  106

Met Arg Trp Cys Leu Leu Leu Ile Trp Ala Gln Gly Leu Arg Gln Ala 
1               5                   10                  15      


Pro Leu Ala Ser Gly Met Met Thr Gly Thr Ile Glu Thr Thr Gly Asn 
            20                  25                  30          


Ile Ser Ala Glu Lys Gly Gly Ser Ile Ile Leu Gln Cys His Leu Ser 
        35                  40                  45              


Ser Thr Thr Ala Gln Val Thr Gln Val Asn Trp Glu Gln Gln Asp Gln 
    50                  55                  60                  


Leu Leu Ala Ile Cys Asn Ala Asp Leu Gly Trp His Ile Ser Pro Ser 
65                  70                  75                  80  


Phe Lys Asp Arg Val Ala Pro Gly Pro Gly Leu Gly Leu Thr Leu Gln 
                85                  90                  95      


Ser Leu Thr Val Asn Asp Thr Gly Glu Tyr Phe Cys Ile Tyr His Thr 
            100                 105                 110         


Tyr Pro Asp Gly Thr Tyr Thr Gly Arg Ile Phe Leu Glu Val Leu Glu 
        115                 120                 125             


Ser Ser Val Ala Glu His Gly Ala Arg Phe Gln Ile Pro Leu Leu Gly 
    130                 135                 140                 


Ala Met Ala Ala Thr Leu Val Val Ile Cys Thr Ala Val Ile Val Val 
145                 150                 155                 160 


Val Ala Leu Thr Arg Lys Lys Lys Ala Leu Arg Ile His Ser Val Glu 
                165                 170                 175     


Gly Asp Leu Arg Arg Lys Ser Ala Gly Gln Glu Glu Trp Ser Pro Ser 
            180                 185                 190         


Ala Pro Ser Pro Pro Gly Ser Cys Val Gln Ala Glu Ala Ala Pro Ala 
        195                 200                 205             


Gly Leu Cys Gly Glu Gln Arg Gly Glu Asp Cys Ala Glu Leu His Asp 
    210                 215                 220                 


Tyr Phe Asn Val Leu Ser Tyr Arg Ser Leu Gly Asn Cys Ser Phe Phe 
225                 230                 235                 240 


Thr Glu Thr Gly 
                


<210>  107
<211>  112
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TIGIT, AA 22-134 NO MUTATIONS (Q495A1 Uniprot ID) AA SEQUENCE

<400>  107

Met Thr Gly Thr Ile Glu Thr Thr Gly Asn Ile Ser Ala Glu Lys Gly 
1               5                   10                  15      


Gly Ser Ile Ile Leu Gln Cys His Leu Ser Ser Thr Thr Ala Gln Val 
            20                  25                  30          


Thr Gln Val Asn Trp Glu Gln Gln Asp Gln Leu Leu Ala Ile Cys Asn 
        35                  40                  45              


Ala Asp Leu Gly Trp His Ile Ser Pro Ser Phe Lys Asp Arg Val Ala 
    50                  55                  60                  


Pro Gly Pro Gly Leu Gly Leu Thr Leu Gln Ser Leu Thr Val Asn Asp 
65                  70                  75                  80  


Thr Gly Glu Tyr Phe Cys Ile Tyr His Thr Tyr Pro Asp Gly Thr Tyr 
                85                  90                  95      


Thr Gly Arg Ile Phe Leu Glu Val Leu Glu Ser Ser Val Ala Glu His 
            100                 105                 110         


<210>  108
<211>  336
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TIGIT, ECD AA 22-134 NO MUTATIONS (Q495A1 Uniprot ID) NA SEQUENCE

<400>  108
atgaccggca caatcgagac aacaggcaac atctctgccg agaagggagg cagcatcatc       60

ctgcagtgcc acctgagcag caccacagcc caggtgaccc aggtgaactg ggagcagcag      120

gaccagctgc tggccatctg caatgccgat ctgggctggc acatcagccc ctcctttaag      180

gatagggtgg cacctggacc aggcctgggc ctgaccctgc agagcctgac cgtgaatgac      240

acaggcgagt acttctgtat ctaccacaca tatcctgatg gcacctatac aggcagaatc      300

tttctggagg tgctggagtc tagcgtggcc gagcac                                336


<210>  109
<211>  112
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TIGIT, AA 22-134 plus I42A C69S (Q495A1 Uniprot ID) AA SEQUENCE

<400>  109

Met Thr Gly Thr Ile Glu Thr Thr Gly Asn Ile Ser Ala Glu Lys Gly 
1               5                   10                  15      


Gly Ser Ile Ala Leu Gln Cys His Leu Ser Ser Thr Thr Ala Gln Val 
            20                  25                  30          


Thr Gln Val Asn Trp Glu Gln Gln Asp Gln Leu Leu Ala Ile Ser Asn 
        35                  40                  45              


Ala Asp Leu Gly Trp His Ile Ser Pro Ser Phe Lys Asp Arg Val Ala 
    50                  55                  60                  


Pro Gly Pro Gly Leu Gly Leu Thr Leu Gln Ser Leu Thr Val Asn Asp 
65                  70                  75                  80  


Thr Gly Glu Tyr Phe Cys Ile Tyr His Thr Tyr Pro Asp Gly Thr Tyr 
                85                  90                  95      


Thr Gly Arg Ile Phe Leu Glu Val Leu Glu Ser Ser Val Ala Glu His 
            100                 105                 110         


<210>  110
<211>  336
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TIGIT, AA 22-134 plus I42A C69S (Q495A1 Uniprot ID) NA SEQUENCE

<400>  110
atgaccggca caatcgagac aacaggcaac atctctgccg agaagggagg cagcatcgcc       60

ctgcagtgcc acctgagcag caccacagcc caggtgaccc aggtgaactg ggagcagcag      120

gaccagctgc tggccatctc caatgccgat ctgggctggc acatcagccc ctcctttaag      180

gatagggtgg cacctggacc aggcctgggc ctgaccctgc agagcctgac cgtgaatgac      240

acaggcgagt acttctgtat ctaccacaca tatcctgatg gcacctatac aggcagaatc      300

tttctggagg tgctggagtc tagcgtggcc gagcac                                336


<210>  111
<211>  112
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TIGIT, AA 22-134 C69S (Q495A1 Uniprot ID in DSP 502V2) 112 AA, 
       SEQUENCE

<400>  111

Met Thr Gly Thr Ile Glu Thr Thr Gly Asn Ile Ser Ala Glu Lys Gly 
1               5                   10                  15      


Gly Ser Ile Ile Leu Gln Cys His Leu Ser Ser Thr Thr Ala Gln Val 
            20                  25                  30          


Thr Gln Val Asn Trp Glu Gln Gln Asp Gln Leu Leu Ala Ile Ser Asn 
        35                  40                  45              


Ala Asp Leu Gly Trp His Ile Ser Pro Ser Phe Lys Asp Arg Val Ala 
    50                  55                  60                  


Pro Gly Pro Gly Leu Gly Leu Thr Leu Gln Ser Leu Thr Val Asn Asp 
65                  70                  75                  80  


Thr Gly Glu Tyr Phe Cys Ile Tyr His Thr Tyr Pro Asp Gly Thr Tyr 
                85                  90                  95      


Thr Gly Arg Ile Phe Leu Glu Val Leu Glu Ser Ser Val Ala Glu His 
            100                 105                 110         


<210>  112
<211>  336
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TIGIT, AA 22-134 C69S (Q495A1 Uniprot ID in DSP 502V2)336 NA, 
       SEQUENCE

<400>  112
atgaccggca caatcgagac aacaggcaac atctctgccg agaagggagg cagcatcatc       60

ctgcagtgcc acctgagcag caccacagcc caggtgaccc aggtgaactg ggagcagcag      120

gaccagctgc tggccatctc caatgccgat ctgggctggc acatcagccc ctcctttaag      180

gatagggtgg cacctggacc aggcctgggc ctgaccctgc agagcctgac cgtgaatgac      240

acaggcgagt acttctgtat ctaccacaca tatcctgatg gcacctatac aggcagaatc      300

tttctggagg tgctggagtc tagcgtggcc gagcac                                336


<210>  113
<211>  116
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TIGIT, ECD AA 22-137 (Q495A1 Uniprot ID in DSP 502V3) 116 AA, 
       SEQUENCE

<400>  113

Met Met Thr Gly Thr Ile Glu Thr Thr Gly Asn Ile Ser Ala Glu Lys 
1               5                   10                  15      


Gly Gly Ser Ile Ile Leu Gln Cys His Leu Ser Ser Thr Thr Ala Gln 
            20                  25                  30          


Val Thr Gln Val Asn Trp Glu Gln Gln Asp Gln Leu Leu Ala Ile Cys 
        35                  40                  45              


Asn Ala Asp Leu Gly Trp His Ile Ser Pro Ser Phe Lys Asp Arg Val 
    50                  55                  60                  


Ala Pro Gly Pro Gly Leu Gly Leu Thr Leu Gln Ser Leu Thr Val Asn 
65                  70                  75                  80  


Asp Thr Gly Glu Tyr Phe Cys Ile Tyr His Thr Tyr Pro Asp Gly Thr 
                85                  90                  95      


Tyr Thr Gly Arg Ile Phe Leu Glu Val Leu Glu Ser Ser Val Ala Glu 
            100                 105                 110         


His Gly Ala Arg 
        115     


<210>  114
<211>  348
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TIGIT, ECD AA 22-137 (Q495A1 Uniprot ID in DSP 502V3) 348 NA, 
       SEQUENCE

<400>  114
atgatgaccg gcactattga aactaccggc aacatctctg ccgagaaggg cggcagcatc       60

atcctccagt gccacctgag cagcaccaca gcccaggtga cacaggtgaa ctgggagcag      120

caggaccagc tgctggccat ctgtaatgcc gatctgggct ggcacatcag cccttccttc      180

aaggacaggg tggcccctgg cccaggcctg ggcctgaccc tccagagcct gaccgtgaat      240

gacacaggcg agtacttctg catctaccac acatatccag atggcaccta tacaggccgg      300

atctttctgg aggtgctgga gtctagcgtg gcagagcacg gcgccaga                   348


<210>  115
<211>  120
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TIGIT full ECD AA 22-141 (Q495A1 Uniprot ID) 120 AA, SEQUENCE

<400>  115

Met Met Thr Gly Thr Ile Glu Thr Thr Gly Asn Ile Ser Ala Glu Lys 
1               5                   10                  15      


Gly Gly Ser Ile Ile Leu Gln Cys His Leu Ser Ser Thr Thr Ala Gln 
            20                  25                  30          


Val Thr Gln Val Asn Trp Glu Gln Gln Asp Gln Leu Leu Ala Ile Cys 
        35                  40                  45              


Asn Ala Asp Leu Gly Trp His Ile Ser Pro Ser Phe Lys Asp Arg Val 
    50                  55                  60                  


Ala Pro Gly Pro Gly Leu Gly Leu Thr Leu Gln Ser Leu Thr Val Asn 
65                  70                  75                  80  


Asp Thr Gly Glu Tyr Phe Cys Ile Tyr His Thr Tyr Pro Asp Gly Thr 
                85                  90                  95      


Tyr Thr Gly Arg Ile Phe Leu Glu Val Leu Glu Ser Ser Val Ala Glu 
            100                 105                 110         


His Gly Ala Arg Phe Gln Ile Pro 
        115                 120 


<210>  116
<211>  21
<212>  PRT
<213>  Artificial sequence

<220>
<223>  artificial signal peptide

<400>  116

Met Glu Ser Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 
1               5                   10                  15      


Asp Gly Val His Ala 
            20      


<210>  117
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker amino acid sequence

<400>  117

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  118
<211>  20
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker amino acid sequence

<400>  118

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser 
            20  


<210>  119
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker amino acid sequence

<400>  119

Gly Gly Gly Gly Gly Gly Gly Gly 
1               5               


<210>  120
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker amino acid sequence

<400>  120

Gly Gly Gly Gly Gly Gly 
1               5       


<210>  121
<211>  14
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker amino acid sequence

<400>  121

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
1               5                   10                  


<210>  122
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker amino acid sequence

<400>  122

Gly Gly Gly Gly Ser Gly Gly Gly Gly 
1               5                   


<210>  123
<211>  20
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker amino acid sequence


<220>
<221>  REPEAT
<222>  (6)..(10)
<223>  may be absent

<220>
<221>  REPEAT
<222>  (11)..(15)
<223>  may be absent

<220>
<221>  REPEAT
<222>  (16)..(20)
<223>  may be absent

<400>  123

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser 
            20  


<210>  124
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker amino acid sequence

<400>  124

Gly Gly Gly Gly Ser 
1               5   


<210>  125
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker amino acid sequence

<400>  125

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


<210>  126
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker amino acid sequence


<220>
<221>  REPEAT
<222>  (6)..(10)
<223>  may be absent

<220>
<221>  REPEAT
<222>  (11)..(15)
<223>  may be absent

<400>  126

Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
1               5                   10                  15  


<210>  127
<211>  27
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker amino acid sequence


<220>
<221>  REPEAT
<222>  (12)..(16)
<223>  may be absent

<220>
<221>  REPEAT
<222>  (17)..(21)
<223>  may be absent

<220>
<221>  REPEAT
<222>  (22)..(26)
<223>  may be absent

<400>  127

Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
1               5                   10                  15      


Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala 
            20                  25          


<210>  128
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker amino acid sequence

<400>  128

Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala 
1               5                   10          


<210>  129
<211>  46
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker amino acid sequence

<400>  129

Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
1               5                   10                  15      


Glu Ala Ala Ala Lys Ala Leu Glu Ala Glu Ala Ala Ala Lys Glu Ala 
            20                  25                  30          


Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala 
        35                  40                  45      


<210>  130
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker amino acid sequence

<400>  130

Pro Ala Pro Ala Pro 
1               5   


<210>  131
<211>  18
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker amino acid sequence

<400>  131

Lys Glu Ser Gly Ser Val Ser Ser Glu Gln Leu Ala Gln Phe Arg Ser 
1               5                   10                  15      


Leu Asp 
        


<210>  132
<211>  14
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker amino acid sequence

<400>  132

Glu Gly Lys Ser Ser Gly Ser Gly Ser Glu Ser Lys Ser Thr 
1               5                   10                  


<210>  133
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker amino acid sequence

<400>  133

Gly Ser Ala Gly Ser Ala Ala Gly Ser Gly Glu Phe 
1               5                   10          


<210>  134
<211>  229
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hIgG4 Fc linker 229 aa

<400>  134

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         


Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            180                 185                 190         


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 


Leu Ser Leu Gly Lys 
225                 


<210>  135
<211>  229
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hIgG4 Fc linker (knob) 229 aa

<400>  135

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      


Glu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         


Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             


Gln Val Cys Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 


Val Ser Leu Trp Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            180                 185                 190         


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 


Leu Ser Leu Gly Lys 
225                 


<210>  136
<211>  229
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hIgG4 Fc linker (hole) 229 aa

<400>  136

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      


Glu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         


Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Thr Leu Pro Pro Ser Gln Cys Glu Met Thr Lys Asn Gln 
    130                 135                 140                 


Val Ser Leu Ser Cys Ala Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Val Ser Arg Leu 
            180                 185                 190         


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 


Leu Ser Leu Gly Lys 
225                 


<210>  137
<211>  232
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hIgG1 Fc linker , (AA 99-330 fromP01857-1 plus  C220S (kabat) 
       substitution 232 amino acids.

<400>  137

Glu Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    130                 135                 140                 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 


Ser Leu Ser Leu Ser Pro Gly Lys 
225                 230         


<210>  138
<211>  359
<212>  PRT
<213>  Artificial sequence

<220>
<223>  short SIRPa- Fc (IgG1 knob)359 AA, first monomer of DSP216V3

<400>  138

Glu Glu Glu Leu Gln Val Ile Gln Pro Asp Lys Ser Val Leu Val Ala 
1               5                   10                  15      


Ala Gly Glu Thr Ala Thr Leu Arg Cys Thr Ala Thr Ser Leu Ile Pro 
            20                  25                  30          


Val Gly Pro Ile Gln Trp Phe Arg Gly Ala Gly Pro Gly Arg Glu Leu 
        35                  40                  45              


Ile Tyr Asn Gln Lys Glu Gly His Phe Pro Arg Val Thr Thr Val Ser 
    50                  55                  60                  


Asp Leu Thr Lys Arg Asn Asn Met Asp Phe Ser Ile Arg Ile Gly Asn 
65                  70                  75                  80  


Ile Thr Pro Ala Asp Ala Gly Thr Tyr Tyr Cys Val Lys Phe Arg Lys 
                85                  90                  95      


Gly Ser Pro Asp Asp Val Glu Phe Lys Ser Gly Ala Gly Thr Glu Leu 
            100                 105                 110         


Ser Val Arg Ala Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
        115                 120                 125             


Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro 
    130                 135                 140                 


Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
145                 150                 155                 160 


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
                165                 170                 175     


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
            180                 185                 190         


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
        195                 200                 205             


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
    210                 215                 220                 


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
225                 230                 235                 240 


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
                245                 250                 255     


Glu Pro Gln Val Tyr Thr Leu Pro Pro Cys Arg Asp Glu Leu Thr Lys 
            260                 265                 270         


Asn Gln Val Ser Leu Trp Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
        275                 280                 285             


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
    290                 295                 300                 


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
305                 310                 315                 320 


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
                325                 330                 335     


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
            340                 345                 350         


Leu Ser Leu Ser Pro Gly Lys 
        355                 


<210>  139
<211>  1077
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA of #138

<400>  139
gaggaggagc tgcaggtcat ccagcccgat aagtctgtgc tggtggcagc aggagagacc       60

gccacactga ggtgcaccgc cacaagcctg atcccagtgg gaccaatcca gtggtttagg      120

ggagcaggcc ctggcagaga gctgatctac aaccagaagg agggccactt cccaagagtg      180

accacagtga gcgacctgac caagcggaac aatatggatt tttccatcag aatcggcaat      240

atcacacctg ccgacgccgg cacctactat tgcgtgaagt tcaggaaggg ctccccagac      300

gatgtggagt ttaagagcgg agcaggcacc gagctgtccg tgcgggcaaa gggaggagga      360

ggcagcggag gaggaggctc cgagcctaag agctccgaca agacccacac atgcccacca      420

tgtcctgcac cagagctgct gggaggacct tccgtgttcc tgtttcctcc aaagccaaag      480

gatacactga tgatctccag aacaccagag gtgacctgcg tggtggtgga cgtgtctcac      540

gaggaccccg aggtgaagtt taactggtac gtggacggcg tggaggtgca caatgccaag      600

accaagccaa gggaggagca gtacaactcc acatatcgcg tggtgtctgt gctgaccgtg      660

ctgcaccagg attggctgaa cggcaaggag tataagtgta aggtgagcaa taaggccctg      720

cccgccccta tcgagaagac catctccaag gcaaagggac agcccaggga gcctcaggtg      780

tacacactgc ccccttgccg cgacgagctg accaagaacc aggtgtctct gtggtgtctg      840

gtgaagggct tctacccatc tgacatcgcc gtggagtggg agagcaatgg ccagcccgag      900

aacaattaca agaccacacc acccgtgctg gacagcgatg gctccttctt tctgtattcc      960

aagctgacag tggacaagtc tcggtggcag cagggcaacg tgttttcctg ttctgtgatg     1020

cacgaggccc tgcacaatca ctatacccag aagagcctgt ccctgtctcc cggcaag        1077


<210>  140
<211>  356
<212>  PRT
<213>  Artificial sequence

<220>
<223>  short SIRPa- Fc (IgG4 knob)356 AA, first monomer of DSP216V4

<400>  140

Glu Glu Glu Leu Gln Val Ile Gln Pro Asp Lys Ser Val Leu Val Ala 
1               5                   10                  15      


Ala Gly Glu Thr Ala Thr Leu Arg Cys Thr Ala Thr Ser Leu Ile Pro 
            20                  25                  30          


Val Gly Pro Ile Gln Trp Phe Arg Gly Ala Gly Pro Gly Arg Glu Leu 
        35                  40                  45              


Ile Tyr Asn Gln Lys Glu Gly His Phe Pro Arg Val Thr Thr Val Ser 
    50                  55                  60                  


Asp Leu Thr Lys Arg Asn Asn Met Asp Phe Ser Ile Arg Ile Gly Asn 
65                  70                  75                  80  


Ile Thr Pro Ala Asp Ala Gly Thr Tyr Tyr Cys Val Lys Phe Arg Lys 
                85                  90                  95      


Gly Ser Pro Asp Asp Val Glu Phe Lys Ser Gly Ala Gly Thr Glu Leu 
            100                 105                 110         


Ser Val Arg Ala Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
        115                 120                 125             


Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Glu 
    130                 135                 140                 


Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
145                 150                 155                 160 


Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
                165                 170                 175     


Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu 
            180                 185                 190         


Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr 
        195                 200                 205             


Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 
    210                 215                 220                 


Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser 
225                 230                 235                 240 


Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln 
                245                 250                 255     


Val Cys Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val 
            260                 265                 270         


Ser Leu Trp Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
        275                 280                 285             


Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
    290                 295                 300                 


Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr 
305                 310                 315                 320 


Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val 
                325                 330                 335     


Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
            340                 345                 350         


Ser Leu Gly Lys 
        355     


<210>  141
<211>  1068
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA of #140

<400>  141
gaggaggagc tgcaggtcat ccagcccgat aagtctgtgc tggtggcagc aggagagacc       60

gccacactga ggtgcaccgc cacaagcctg atcccagtgg gaccaatcca gtggtttagg      120

ggagcaggcc ctggcagaga gctgatctac aaccagaagg agggccactt cccaagagtg      180

accacagtga gcgacctgac caagcggaac aatatggatt tttccatcag aatcggcaat      240

atcacacctg ccgacgccgg cacctactat tgcgtgaagt tcaggaaggg ctccccagac      300

gatgtggagt ttaagagcgg agcaggcacc gagctgtccg tgcgggcaaa gggaggagga      360

ggatccggag gaggaggatc cgagtctaag tatggaccac catgccctcc atgtccagca      420

cctgagtttg agggaggacc tagcgtgttc ctgtttcccc ctaagccaaa ggacacactg      480

atgatctcca ggacaccaga ggtgacctgc gtggtggtgg acgtgtctca ggaggatccc      540

gaggtgcagt tcaactggta cgtggatggc gtggaggtgc acaatgccaa gaccaagcct      600

agggaggagc agtttaactc tacataccgc gtggtgagcg tgctgaccgt gctgcaccag      660

gattggctga acggcaagga gtataagtgt aaggtgagca ataagggcct gccaagctcc      720

atcgagaaga ccatctccaa ggcaaaggga cagccaaggg agcctcaggt gtgcacactg      780

ccaccctctc aggaggagat gaccaagaac caggtgagcc tgtggtgtct ggtgaagggc      840

ttctacccaa gcgacatcgc cgtggagtgg gagtccaatg gccagcccga gaacaattac      900

aagaccacac ctccagtgct ggactctgat ggcagcttct ttctgtattc taggctgaca      960

gtggataaga gccgctggca ggagggcaac gtgtttagct gttccgtgat gcacgaggcc     1020

ctgcacaatc actataccca gaagtctctg agcctgtccc tgggcaag                  1068


<210>  142
<211>  585
<212>  PRT
<213>  Artificial sequence

<220>
<223>  SIRPa- Fc (IgG1 knob LALA)358 AA, first monomer of DSP216V5

<400>  142

Glu Glu Glu Leu Gln Val Ile Gln Pro Asp Lys Ser Val Leu Val Ala 
1               5                   10                  15      


Ala Gly Glu Thr Ala Thr Leu Arg Cys Thr Ala Thr Ser Leu Ile Pro 
            20                  25                  30          


Val Gly Pro Ile Gln Trp Phe Arg Gly Ala Gly Pro Gly Arg Glu Leu 
        35                  40                  45              


Ile Tyr Asn Gln Lys Glu Gly His Phe Pro Arg Val Thr Thr Val Ser 
    50                  55                  60                  


Asp Leu Thr Lys Arg Asn Asn Met Asp Phe Ser Ile Arg Ile Gly Asn 
65                  70                  75                  80  


Ile Thr Pro Ala Asp Ala Gly Thr Tyr Tyr Cys Val Lys Phe Arg Lys 
                85                  90                  95      


Gly Ser Pro Asp Asp Val Glu Phe Lys Ser Gly Ala Gly Thr Glu Leu 
            100                 105                 110         


Ser Val Arg Ala Lys Pro Ser Ala Pro Val Val Ser Gly Pro Ala Ala 
        115                 120                 125             


Arg Ala Thr Pro Gln His Thr Val Ser Phe Thr Cys Glu Ser His Gly 
    130                 135                 140                 


Phe Ser Pro Arg Asp Ile Thr Leu Lys Trp Phe Lys Asn Gly Asn Glu 
145                 150                 155                 160 


Leu Ser Asp Phe Gln Thr Asn Val Asp Pro Val Gly Glu Ser Val Ser 
                165                 170                 175     


Tyr Ser Ile His Ser Thr Ala Lys Val Val Leu Thr Arg Glu Asp Val 
            180                 185                 190         


His Ser Gln Val Ile Cys Glu Val Ala His Val Thr Leu Gln Gly Asp 
        195                 200                 205             


Pro Leu Arg Gly Thr Ala Asn Leu Ser Glu Thr Ile Arg Val Pro Pro 
    210                 215                 220                 


Thr Leu Glu Val Thr Gln Gln Pro Val Arg Ala Glu Asn Gln Val Asn 
225                 230                 235                 240 


Val Thr Cys Gln Val Arg Lys Phe Tyr Pro Gln Arg Leu Gln Leu Thr 
                245                 250                 255     


Trp Leu Glu Asn Gly Asn Val Ser Arg Thr Glu Thr Ala Ser Thr Val 
            260                 265                 270         


Thr Glu Asn Lys Asp Gly Thr Tyr Asn Trp Met Ser Trp Leu Leu Val 
        275                 280                 285             


Asn Val Ser Ala His Arg Asp Asp Val Lys Leu Thr Cys Gln Val Glu 
    290                 295                 300                 


His Asp Gly Gln Pro Ala Val Ser Lys Ser His Asp Leu Lys Val Ser 
305                 310                 315                 320 


Ala His Pro Lys Glu Gln Gly Ser Asn Thr Ala Ala Glu Asn Thr Gly 
                325                 330                 335     


Ser Asn Glu Arg Asn Ile Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly 
            340                 345                 350         


Ser Glu Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
        355                 360                 365             


Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
    370                 375                 380                 


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
385                 390                 395                 400 


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
                405                 410                 415     


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
            420                 425                 430         


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
        435                 440                 445             


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
    450                 455                 460                 


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
465                 470                 475                 480 


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Cys Arg Asp Glu Leu 
                485                 490                 495     


Thr Lys Asn Gln Val Ser Leu Trp Cys Leu Val Lys Gly Phe Tyr Pro 
            500                 505                 510         


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
        515                 520                 525             


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
    530                 535                 540                 


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
545                 550                 555                 560 


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
                565                 570                 575     


Lys Ser Leu Ser Leu Ser Pro Gly Lys 
            580                 585 


<210>  143
<211>  1755
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA of #142

<400>  143
gaggaggagc tgcaggtcat ccagcccgat aagtctgtgc tggtggcagc aggagagacc       60

gccacactga ggtgcaccgc cacaagcctg atcccagtgg gaccaatcca gtggtttagg      120

ggagcaggcc ctggcagaga gctgatctac aaccagaagg agggccactt cccaagagtg      180

accacagtga gcgacctgac caagcggaac aatatggatt tttccatcag aatcggcaat      240

atcacacctg ccgacgccgg cacctactat tgcgtgaagt tcaggaaggg ctccccagac      300

gatgtggagt ttaagagcgg agcaggcacc gagctgtccg tgcgggcaaa gccttccgcc      360

ccagtggtgt ctggaccagc agccagagcc accccacagc acacagtgtc cttcacctgt      420

gagtctcacg gctttagccc ccgggacatc accctgaagt ggttcaagaa cggcaatgag      480

ctgtctgact ttcagaccaa cgtggacccc gtgggcgagt ctgtgagcta ttccatccac      540

tctacagcca aggtggtgct gacccgcgag gacgtgcaca gccaggtcat ctgcgaggtg      600

gcacacgtga ccctgcaggg cgatcctctg aggggcacag ccaatctgag cgagaccatc      660

agagtgcccc ctacactgga ggtgacccag cagcccgtgc gcgcagagaa ccaagtgaat      720

gtgacatgtc aggtgaggaa gttctaccct cagcgcctgc agctgacctg gctggagaac      780

ggcaacgtga gccggaccga gacagccagc accgtgacag agaacaagga cggcacatat      840

aattggatgt cttggctgct ggtgaacgtg agcgcccaca gggacgatgt gaagctgacc      900

tgccaggtgg agcacgacgg acagccagcc gtgtctaaga gccacgatct gaaggtgagc      960

gcccacccta aggagcaggg ctccaacaca gccgccgaga ataccggcag caacgagcgg     1020

aatatctacg gaggaggagg cagcggagga ggaggctccg agcctaagag ctccgacaag     1080

acccacacat gcccaccatg tcctgcacca gaggcagcag gaggaccttc cgtgttcctg     1140

tttcctccaa agccaaagga tacactgatg atctccagaa caccagaggt gacctgcgtg     1200

gtggtggacg tgtctcacga ggaccccgag gtgaagttta actggtacgt ggacggcgtg     1260

gaggtgcaca atgccaagac caagccaagg gaggagcagt acaactccac atatcgcgtg     1320

gtgtctgtgc tgaccgtgct gcaccaggat tggctgaacg gcaaggagta taagtgtaag     1380

gtgagcaata aggccctgcc cgcccctatc gagaagacca tctccaaggc aaagggacag     1440

cccagggagc ctcaggtgta cacactgccc ccttgccgcg acgagctgac caagaaccag     1500

gtgtctctgt ggtgtctggt gaagggcttc tacccatctg acatcgccgt ggagtgggag     1560

agcaatggcc agcccgagaa caattacaag accacaccac ccgtgctgga cagcgatggc     1620

tccttctttc tgtattccaa gctgacagtg gacaagtctc ggtggcagca gggcaacgtg     1680

ttttcctgtt ctgtgatgca cgaggccctg cacaatcact atacccagaa gagcctgtcc     1740

ctgtctcccg gcaag                                                      1755


<210>  144
<211>  359
<212>  PRT
<213>  Artificial sequence

<220>
<223>  short SIRPa- Fc (IgG1 knob LALA )359 AA, first monomer of 
       DSP216V6

<400>  144

Glu Glu Glu Leu Gln Val Ile Gln Pro Asp Lys Ser Val Leu Val Ala 
1               5                   10                  15      


Ala Gly Glu Thr Ala Thr Leu Arg Cys Thr Ala Thr Ser Leu Ile Pro 
            20                  25                  30          


Val Gly Pro Ile Gln Trp Phe Arg Gly Ala Gly Pro Gly Arg Glu Leu 
        35                  40                  45              


Ile Tyr Asn Gln Lys Glu Gly His Phe Pro Arg Val Thr Thr Val Ser 
    50                  55                  60                  


Asp Leu Thr Lys Arg Asn Asn Met Asp Phe Ser Ile Arg Ile Gly Asn 
65                  70                  75                  80  


Ile Thr Pro Ala Asp Ala Gly Thr Tyr Tyr Cys Val Lys Phe Arg Lys 
                85                  90                  95      


Gly Ser Pro Asp Asp Val Glu Phe Lys Ser Gly Ala Gly Thr Glu Leu 
            100                 105                 110         


Ser Val Arg Ala Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
        115                 120                 125             


Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro 
    130                 135                 140                 


Glu Ala Ala Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
145                 150                 155                 160 


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
                165                 170                 175     


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
            180                 185                 190         


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
        195                 200                 205             


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
    210                 215                 220                 


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
225                 230                 235                 240 


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
                245                 250                 255     


Glu Pro Gln Val Tyr Thr Leu Pro Pro Cys Arg Asp Glu Leu Thr Lys 
            260                 265                 270         


Asn Gln Val Ser Leu Trp Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
        275                 280                 285             


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
    290                 295                 300                 


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
305                 310                 315                 320 


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
                325                 330                 335     


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
            340                 345                 350         


Leu Ser Leu Ser Pro Gly Lys 
        355                 


<210>  145
<211>  1077
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA of #144

<400>  145
gaggaggagc tgcaggtcat ccagcccgat aagtctgtgc tggtggcagc aggagagacc       60

gccacactga ggtgcaccgc cacaagcctg atcccagtgg gaccaatcca gtggtttagg      120

ggagcaggcc ctggcagaga gctgatctac aaccagaagg agggccactt cccaagagtg      180

accacagtga gcgacctgac caagcggaac aatatggatt tttccatcag aatcggcaat      240

atcacacctg ccgacgccgg cacctactat tgcgtgaagt tcaggaaggg ctccccagac      300

gatgtggagt ttaagagcgg agcaggcacc gagctgtccg tgcgggcaaa gggaggagga      360

ggcagcggag gaggaggctc cgagcctaag agctccgaca agacccacac atgcccacca      420

tgtcctgcac cagaggcagc aggaggacct tccgtgttcc tgtttcctcc aaagccaaag      480

gatacactga tgatctccag aacaccagag gtgacctgcg tggtggtgga cgtgtctcac      540

gaggaccccg aggtgaagtt taactggtac gtggacggcg tggaggtgca caatgccaag      600

accaagccaa gggaggagca gtacaactcc acatatcgcg tggtgtctgt gctgaccgtg      660

ctgcaccagg attggctgaa cggcaaggag tataagtgta aggtgagcaa taaggccctg      720

cccgccccta tcgagaagac catctccaag gcaaagggac agcccaggga gcctcaggtg      780

tacacactgc ccccttgccg cgacgagctg accaagaacc aggtgtctct gtggtgtctg      840

gtgaagggct tctacccatc tgacatcgcc gtggagtggg agagcaatgg ccagcccgag      900

aacaattaca agaccacacc acccgtgctg gacagcgatg gctccttctt tctgtattcc      960

aagctgacag tggacaagtc tcggtggcag cagggcaacg tgttttcctg ttctgtgatg     1020

cacgaggccc tgcacaatca ctatacccag aagagcctgt ccctgtctcc cggcaag        1077


<210>  146
<211>  354
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TIGIT- Fc (IgG1 knob LALA )354 AA, first monomer of DSP502V4

<400>  146

Met Thr Gly Thr Ile Glu Thr Thr Gly Asn Ile Ser Ala Glu Lys Gly 
1               5                   10                  15      


Gly Ser Ile Ala Leu Gln Cys His Leu Ser Ser Thr Thr Ala Gln Val 
            20                  25                  30          


Thr Gln Val Asn Trp Glu Gln Gln Asp Gln Leu Leu Ala Ile Ser Asn 
        35                  40                  45              


Ala Asp Leu Gly Trp His Ile Ser Pro Ser Phe Lys Asp Arg Val Ala 
    50                  55                  60                  


Pro Gly Pro Gly Leu Gly Leu Thr Leu Gln Ser Leu Thr Val Asn Asp 
65                  70                  75                  80  


Thr Gly Glu Tyr Phe Cys Ile Tyr His Thr Tyr Pro Asp Gly Thr Tyr 
                85                  90                  95      


Thr Gly Arg Ile Phe Leu Glu Val Leu Glu Ser Ser Val Ala Glu His 
            100                 105                 110         


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Pro Lys Ser Ser Asp 
        115                 120                 125             


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly 
    130                 135                 140                 


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
145                 150                 155                 160 


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
                165                 170                 175     


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
            180                 185                 190         


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 
        195                 200                 205             


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
    210                 215                 220                 


Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
225                 230                 235                 240 


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
                245                 250                 255     


Thr Leu Pro Pro Cys Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu 
            260                 265                 270         


Trp Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
        275                 280                 285             


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
    290                 295                 300                 


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
305                 310                 315                 320 


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
                325                 330                 335     


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
            340                 345                 350         


Gly Lys 
        


<210>  147
<211>  1062
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA of #146

<400>  147
atgaccggca caatcgagac aacaggcaac atctctgccg agaagggagg cagcatcgcc       60

ctgcagtgcc acctgagcag caccacagcc caggtgaccc aggtgaactg ggagcagcag      120

gaccagctgc tggccatctc taatgccgat ctgggctggc acatcagccc atcctttaag      180

gatagggtgg caccaggacc aggcctgggc ctgaccctgc agagcctgac cgtgaatgac      240

acaggcgagt acttctgtat ctaccacaca tatcccgatg gcacctatac aggcagaatc      300

tttctggagg tgctggagtc tagcgtggcc gagcacggag gaggaggcag cggaggagga      360

ggctccgagc ctaagtcctc tgacaagacc cacacatgcc ccccttgtcc tgcaccagag      420

gcagcaggcg gaccttccgt gttcctgttt ccacccaagc caaaggatac cctgatgatc      480

tccaggaccc ctgaggtgac atgcgtggtg gtggacgtgt ctcacgagga ccccgaggtg      540

aagttcaact ggtacgtgga cggcgtggag gtgcacaatg ccaagacaaa gcctcgggag      600

gagcagtaca actccaccta tagagtggtg tctgtgctga cagtgctgca ccaggattgg      660

ctgaacggca aggagtataa gtgtaaggtg agcaataagg ccctgcccgc ccctatcgag      720

aaaaccatca gcaaggcaaa gggacagcca agggagccac aggtgtacac cctgcctcca      780

tgccgcgacg agctgacaaa gaaccaggtg agcctgtggt gtctggtgaa gggcttctat      840

ccatctgaca tcgccgtgga gtgggagagc aatggccagc ccgagaacaa ttacaagacc      900

acaccccctg tgctggactc cgatggctct ttctttctgt atagcaagct gaccgtggac      960

aagtccagat ggcagcaggg caacgtgttt tcttgcagcg tgatgcacga ggccctgcac     1020

aatcactaca cacagaagtc cctgtctctg agccccggca ag                        1062


<210>  148
<211>  382
<212>  PRT
<213>  Artificial sequence

<220>
<223>  PD1- Fc (IgG1 hole LALA )382 AA, second monomer of DSP502V4

<400>  148

Asp Ser Pro Asp Arg Pro Trp Asn Pro Pro Thr Phe Ser Pro Ala Leu 
1               5                   10                  15      


Leu Val Val Thr Glu Gly Asp Asn Ala Thr Phe Thr Cys Ser Phe Ser 
            20                  25                  30          


Asn Thr Ser Glu Ser Phe Val Leu Asn Trp Tyr Arg Met Ser Pro Ser 
        35                  40                  45              


Asn Gln Thr Asp Lys Leu Ala Ala Phe Pro Glu Asp Arg Ser Gln Pro 
    50                  55                  60                  


Gly Gln Asp Ser Arg Phe Arg Val Thr Gln Leu Pro Asn Gly Arg Asp 
65                  70                  75                  80  


Phe His Met Ser Val Val Arg Ala Arg Arg Asn Asp Ser Gly Thr Tyr 
                85                  90                  95      


Leu Cys Gly Ala Ile Ser Leu Ala Pro Lys Ala Gln Ile Lys Glu Ser 
            100                 105                 110         


Leu Arg Ala Glu Leu Arg Val Thr Glu Arg Arg Ala Glu Val Pro Thr 
        115                 120                 125             


Ala His Pro Ser Pro Ser Pro Arg Pro Ala Gly Gln Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Glu Pro Lys Ser Ser Asp Lys Thr His Thr 
145                 150                 155                 160 


Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe 
                165                 170                 175     


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
            180                 185                 190         


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
        195                 200                 205             


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
    210                 215                 220                 


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
225                 230                 235                 240 


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
                245                 250                 255     


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
            260                 265                 270         


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Cys Thr Leu Pro Pro 
        275                 280                 285             


Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Ser Cys Ala Val 
    290                 295                 300                 


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
305                 310                 315                 320 


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
                325                 330                 335     


Gly Ser Phe Phe Leu Val Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
            340                 345                 350         


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
        355                 360                 365             


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    370                 375                 380         


<210>  149
<211>  1146
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA of #148

<400>  149
gattcacccg atagaccttg gaacccacct accttctccc ccgccctgct ggtggtgaca       60

gagggcgaca atgccacctt cacatgctct tttagcaaca cctccgagtc tttcgtgctg      120

aattggtaca ggatgagccc ctccaaccag acagataagc tggccgcatt tccagaggac      180

cgcagccagc caggacagga ttcccggttc agagtgaccc agctgcctaa tggccgggac      240

tttcacatgt ctgtggtgag agcccggaga aacgatagcg gcacatacct gtgcggagcc      300

atctccctgg cccctaaggc acagatcaag gagtccctga gggcagagct gagggtgacc      360

gagaggaggg cagaggtgcc aacagcacac ccttctccaa gcccccggcc tgcaggacag      420

ggaggaggag gctccggcgg cggcggctct gagccaaaga gctccgacaa gacccacaca      480

tgcccaccat gtccagcacc agaggcagca ggaggaccta gcgtgttcct gtttcctcca      540

aagccaaagg ataccctgat gatctctagg accccagagg tgacatgcgt ggtggtggac      600

gtgagccacg aggaccccga ggtgaagttt aattggtacg tggacggcgt ggaggtgcac      660

aacgccaaga caaagcctag ggaggagcag tacaattcta cctatcgcgt ggtgagcgtg      720

ctgacagtgc tgcaccagga ttggctgaat ggcaaggagt ataagtgtaa ggtgtccaac      780

aaggccctgc ctgccccaat cgagaagacc atctctaagg caaagggaca gccccgggag      840

cctcaggtgt gcaccctgcc ccctagcaga gacgagctga caaagaatca ggtgtccctg      900

tcttgtgccg tgaagggctt ctaccccagc gacatcgcag tggagtggga gtccaacgga      960

cagcctgaga acaattataa gaccacacca cccgtgctgg actctgatgg cagcttcttt     1020

ctggtgtcca agctgaccgt ggacaagtct cggtggcagc agggcaacgt gtttagctgc     1080

tccgtgatgc acgaagcact gcacaaccac tacacccaga agtcactgtc actgtcccca     1140

ggaaag                                                                1146


<210>  150
<211>  645
<212>  PRT
<213>  Artificial sequence

<220>
<223>  LILRB2- Fc (IgG1 hole LALA )382 AA, second monomer of DSP216V5 
       and  DSP216V6

<400>  150

Gln Thr Gly Thr Ile Pro Lys Pro Thr Leu Trp Ala Glu Pro Asp Ser 
1               5                   10                  15      


Val Ile Thr Gln Gly Ser Pro Val Thr Leu Ser Cys Gln Gly Ser Leu 
            20                  25                  30          


Glu Ala Gln Glu Tyr Arg Leu Tyr Arg Glu Lys Lys Ser Ala Ser Trp 
        35                  40                  45              


Ile Thr Arg Ile Arg Pro Glu Leu Val Lys Asn Gly Gln Phe His Ile 
    50                  55                  60                  


Pro Ser Ile Thr Trp Glu His Thr Gly Arg Tyr Gly Cys Gln Tyr Tyr 
65                  70                  75                  80  


Ser Arg Ala Arg Trp Ser Glu Leu Ser Asp Pro Leu Val Leu Val Met 
                85                  90                  95      


Thr Gly Ala Tyr Pro Lys Pro Thr Leu Ser Ala Gln Pro Ser Pro Val 
            100                 105                 110         


Val Thr Ser Gly Gly Arg Val Thr Leu Gln Cys Glu Ser Gln Val Ala 
        115                 120                 125             


Phe Gly Gly Phe Ile Leu Cys Lys Glu Gly Glu Glu Glu His Pro Gln 
    130                 135                 140                 


Cys Leu Asn Ser Gln Pro His Ala Arg Gly Ser Ser Arg Ala Ile Phe 
145                 150                 155                 160 


Ser Val Gly Pro Val Ser Pro Asn Arg Arg Trp Ser His Arg Cys Tyr 
                165                 170                 175     


Gly Tyr Asp Leu Asn Ser Pro Tyr Val Trp Ser Ser Pro Ser Asp Leu 
            180                 185                 190         


Leu Glu Leu Leu Val Pro Gly Val Ser Lys Lys Pro Ser Leu Ser Val 
        195                 200                 205             


Gln Pro Gly Pro Val Val Ala Pro Gly Glu Ser Leu Thr Leu Gln Cys 
    210                 215                 220                 


Val Ser Asp Val Gly Tyr Asp Arg Phe Val Leu Tyr Lys Glu Gly Glu 
225                 230                 235                 240 


Arg Asp Leu Arg Gln Leu Pro Gly Arg Gln Pro Gln Ala Gly Leu Ser 
                245                 250                 255     


Gln Ala Asn Phe Thr Leu Gly Pro Val Ser Arg Ser Tyr Gly Gly Gln 
            260                 265                 270         


Tyr Arg Cys Tyr Gly Ala His Asn Leu Ser Ser Glu Cys Ser Ala Pro 
        275                 280                 285             


Ser Asp Pro Leu Asp Ile Leu Ile Thr Gly Gln Ile Arg Gly Thr Pro 
    290                 295                 300                 


Phe Ile Ser Val Gln Pro Gly Pro Thr Val Ala Ser Gly Glu Asn Val 
305                 310                 315                 320 


Thr Leu Leu Cys Gln Ser Trp Arg Gln Phe His Thr Phe Leu Leu Thr 
                325                 330                 335     


Lys Ala Gly Ala Ala Asp Ala Pro Leu Arg Leu Arg Ser Ile His Glu 
            340                 345                 350         


Tyr Pro Lys Tyr Gln Ala Glu Phe Pro Met Ser Pro Val Thr Ser Ala 
        355                 360                 365             


His Ala Gly Thr Tyr Arg Cys Tyr Gly Ser Leu Asn Ser Asp Pro Tyr 
    370                 375                 380                 


Leu Leu Ser His Pro Ser Glu Pro Leu Glu Leu Val Val Ser Gly Gly 
385                 390                 395                 400 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Pro Lys 
                405                 410                 415     


Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Ala 
            420                 425                 430         


Ala Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
        435                 440                 445             


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
    450                 455                 460                 


Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val 
465                 470                 475                 480 


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 
                485                 490                 495     


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
            500                 505                 510         


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 
        515                 520                 525             


Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
    530                 535                 540                 


Gln Val Cys Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln 
545                 550                 555                 560 


Val Ser Leu Ser Cys Ala Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
                565                 570                 575     


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
            580                 585                 590         


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Val Ser Lys Leu 
        595                 600                 605             


Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 
    610                 615                 620                 


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
625                 630                 635                 640 


Leu Ser Pro Gly Lys 
                645 


<210>  151
<211>  2019
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA of #150

<400>  151
tctagagcca ccatggagtc cccagcacag ctgctgttcc tgctgctgct gtggctgcct       60

gacggagtgc acgcacagac cggcacaatc ccaaagccca ccctgtgggc cgagcctgat      120

tccgtgatca cccagggctc tccagtgaca ctgtcctgcc agggctctct ggaggcccag      180

gagtaccggc tgtatagaga gaagaagtct gccagctgga tcacccggat cagacctgag      240

ctggtgaaga acggccagtt tcacatccca agcatcacct gggagcacac aggccggtac      300

ggatgccagt actattcccg ggccagatgg agcgagctgt ccgaccctct ggtgctggtc      360

atgaccggcg cctatcctaa gccaacactg agcgcccagc catcccctgt ggtgaccagc      420

ggcggcagag tgacactgca gtgtgagtcc caggtggcct tcggcggctt tatcctgtgc      480

aaggagggcg aggaggagca cccacagtgt ctgaacagcc agccacacgc ccggggcagc      540

tccagagcca tcttctccgt gggacccgtg agcccaaacc ggagatggag ccaccggtgc      600

tacggctatg acctgaatag cccttacgtg tggtctagcc catccgatct gctggagctg      660

ctggtgcccg gcgtgtccaa gaagccttcc ctgtctgtgc agccaggacc agtggtggca      720

ccaggagagt ctctgaccct gcagtgcgtg agcgacgtgg gctacgatcg gttcgtgctg      780

tataaggagg gagagaggga tctgaggcag ctgccaggca gacagccaca ggccggcctg      840

agccaggcca actttacact gggcccagtg agcaggtcct atggcggaca gtacaggtgc      900

tatggagcac acaatctgtc ctctgagtgt tctgccccca gcgaccccct ggacatcctg      960

atcaccggcc agatcagggg cacacccttc atctccgtgc agcctggacc aaccgtggcc     1020

tctggcgaga acgtgacact gctgtgccag tcttggcgcc agttccacac ctttctgctg     1080

acaaaggcag gagcagcaga cgcaccactg aggctgcgca gcatccacga gtaccccaag     1140

tatcaggccg agtttccaat gtctccagtg accagcgccc acgcaggcac atacaggtgt     1200

tatggcagcc tgaacagcga cccctacctg ctgagccacc cttccgagcc actggagctg     1260

gtggtgagcg gaggaggagg ctccggagga ggaggctctg gcggcggcgg cagcgagcct     1320

aagagctccg acaagaccca cacatgccca ccttgtccag cacctgaggc agcaggagga     1380

ccatccgtgt tcctgtttcc acccaagcct aaggataccc tgatgatctc tcgcacccct     1440

gaggtgacat gcgtggtggt ggacgtgagc cacgaggacc ccgaggtgaa gtttaactgg     1500

tacgtggacg gcgtggaggt gcacaatgcc aagacaaagc cccgggagga gcagtacaac     1560

agcacctata gagtggtgtc cgtgctgaca gtgctgcacc aggattggct gaacggcaag     1620

gagtacaagt gtaaggtgtc caataaggcc ctgccagccc ccatcgagaa gaccatctct     1680

aaggcaaagg gacagcccag ggagcctcag gtgtgcaccc tgcctccaag ccgcgacgag     1740

ctgacaaaga accaggtgtc tctgagctgt gccgtgaagg gcttctaccc atctgacatc     1800

gccgtggagt gggagagcaa tggccagccc gagaacaatt ataagaccac accccctgtg     1860

ctggactctg atggcagctt ctttctggtg tccaagctga ccgtggataa gtctaggtgg     1920

cagcagggca acgtgttttc ctgttctgtg atgcacgagg ccctgcacaa tcactacaca     1980

cagaagagcc tgtccctgtc tcccggcaag tgagatatc                            2019


<210>  152
<211>  232
<212>  PRT
<213>  Artificial sequence

<220>
<223>  IgG1 Fc Hole LALA, Fc (AA 99-330) C220S L234A, L235A plus Hole 
       T366S and L368A Y407V) and Y349C (the numbers from KABAT) used in
       DSP216V5, DSP216V6 and DSP502V4

<400>  152

Glu Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      


Pro Glu Ala Ala Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             


Arg Glu Pro Gln Val Cys Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    130                 135                 140                 


Lys Asn Gln Val Ser Leu Ser Cys Ala Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Val 
            180                 185                 190         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 


Ser Leu Ser Leu Ser Pro Gly Lys 
225                 230         


<210>  153
<211>  696
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA of #152

<400>  153
gagcctaaga gctccgacaa gacccacaca tgcccacctt gtccagcacc tgaggcagca       60

ggaggaccat ccgtgttcct gtttccaccc aagcctaagg ataccctgat gatctctcgc      120

acccctgagg tgacatgcgt ggtggtggac gtgagccacg aggaccccga ggtgaagttt      180

aactggtacg tggacggcgt ggaggtgcac aatgccaaga caaagccccg ggaggagcag      240

tacaacagca cctatagagt ggtgtccgtg ctgacagtgc tgcaccagga ttggctgaac      300

ggcaaggagt acaagtgtaa ggtgtccaat aaggccctgc cagcccccat cgagaagacc      360

atctctaagg caaagggaca gcccagggag cctcaggtgt gcaccctgcc tccaagccgc      420

gacgagctga caaagaacca ggtgtctctg agctgtgccg tgaagggctt ctacccatct      480

gacatcgccg tggagtggga gagcaatggc cagcccgaga acaattataa gaccacaccc      540

cctgtgctgg actctgatgg cagcttcttt ctggtgtcca agctgaccgt ggataagtct      600

aggtggcagc agggcaacgt gttttcctgt tctgtgatgc acgaggccct gcacaatcac      660

tacacacaga agagcctgtc cctgtctccc ggcaag                                696


<210>  154
<211>  232
<212>  PRT
<213>  Artificial sequence

<220>
<223>  IgG1 Fc knob LALA (Fc IgG1 (AA 99-330) P01857-1 C220S (kabat) 
       plus Knob T366W (kabat) and S354C (kabat) plus L234A, L235A 
       (kabat), used in DSP216v5 and DSP502V4

<400>  154

Glu Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      


Pro Glu Ala Ala Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Cys Arg Asp Glu Leu Thr 
    130                 135                 140                 


Lys Asn Gln Val Ser Leu Trp Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 


Ser Leu Ser Leu Ser Pro Gly Lys 
225                 230         


<210>  155
<211>  698
<212>  DNA
<213>  Artificial sequence

<220>
<223>  NA of #154


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a, c, g, or t

<400>  155
nagagcctaa gagctccgac aagacccaca catgcccacc atgtcctgca ccagaggcag       60

caggaggacc ttccgtgttc ctgtttcctc caaagccaaa ggatacactg atgatctcca      120

gaacaccaga ggtgacctgc gtggtggtgg acgtgtctca cgaggacccc gaggtgaagt      180

ttaactggta cgtggacggc gtggaggtgc acaatgccaa gaccaagcca agggaggagc      240

agtacaactc cacatatcgc gtggtgtctg tgctgaccgt gctgcaccag gattggctga      300

acggcaagga gtataagtgt aaggtgagca ataaggccct gcccgcccct atcgagaaga      360

ccatctccaa ggcaaaggga cagcccaggg agcctcaggt gtacacactg cccccttgcc      420

gcgacgagct gaccaagaac caggtgtctc tgtggtgtct ggtgaagggc ttctacccat      480

ctgacatcgc cgtggagtgg gagagcaatg gccagcccga gaacaattac aagaccacac      540

cacccgtgct ggacagcgat ggctccttct ttctgtattc caagctgaca gtggacaagt      600

ctcggtggca gcagggcaac gtgttttcct gttctgtgat gcacgaggcc ctgcacaatc      660

actataccca gaagagcctg tccctgtctc ccggcaag                              698


<210>  156
<211>  232
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hIgG1 Fc

<400>  156

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    130                 135                 140                 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 


Ser Leu Ser Leu Ser Pro Gly Lys 
225                 230         


<210>  157
<211>  229
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hIgG4-SPLE- Fc knob

<400>  157

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      


Glu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         


Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 


Val Ser Leu Trp Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            180                 185                 190         


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 


Leu Ser Leu Gly Lys 
225                 


<210>  158
<211>  229
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hIgG4 Fc knob

<400>  158

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         


Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 


Val Ser Leu Trp Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            180                 185                 190         


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 


Leu Ser Leu Gly Lys 
225                 


<210>  159
<211>  229
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hIgG4 Fc hole

<400>  159

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         


Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 


Val Ser Leu Ser Cys Ala Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Val Ser Arg Leu 
            180                 185                 190         


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 


Leu Ser Leu Gly Lys 
225                 


<210>  160
<211>  232
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hIgG1 Fc knob only

<400>  160

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    130                 135                 140                 


Lys Asn Gln Val Ser Leu Trp Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 


Ser Leu Ser Leu Ser Pro Gly Lys 
225                 230         


<210>  161
<211>  232
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hIgG1 Fc hole only

<400>  161

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    130                 135                 140                 


Lys Asn Gln Val Ser Leu Ser Cys Ala Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Val 
            180                 185                 190         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 


Ser Leu Ser Leu Ser Pro Gly Lys 
225                 230         


<210>  162
<211>  232
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hIgG1 C220S Fc Hole

<400>  162

Glu Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             


Arg Glu Pro Gln Val Cys Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    130                 135                 140                 


Lys Asn Gln Val Ser Leu Ser Cys Ala Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 


Ser Leu Ser Leu Ser Pro Gly Lys 
225                 230         


<210>  163
<211>  229
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hIgG4-SPLE- Fc knob NO Y349C

<400>  163

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      


Glu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         


Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 


Val Ser Leu Trp Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            180                 185                 190         


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 


Leu Ser Leu Gly Lys 
225                 


<210>  164
<211>  229
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hIgG4-SPLE- Fc Hole NO E356C

<400>  164

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      


Glu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         


Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 


Val Ser Leu Ser Cys Ala Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Val Ser Arg Leu 
            180                 185                 190         


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 


Leu Ser Leu Gly Lys 
225                 


