                         SEQUENCE LISTING

<110>  IPSEN BIOPHARM LIMITED
 
<120>  TREATMENT OF BRAIN DAMAGE

<130>  P68751WO

<150>  GB2100566.5
<151>  2021-01-15

<160>  76    

<170>  PatentIn version 3.5

<210>  1
<211>  3888
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide Sequence of rBoNT/A(0)

<400>  1
atgccattcg tcaacaagca attcaactac aaagacccag tcaacggcgt cgacatcgca       60

tacatcaaga ttccgaacgc cggtcaaatg cagccggtta aggcttttaa gatccacaac      120

aagatttggg ttatcccgga gcgtgacacc ttcacgaacc cggaagaagg cgatctgaac      180

ccgccaccgg aagcgaagca agtccctgtc agctactacg attcgacgta cctgagcacg      240

gataacgaaa aagataacta cctgaaaggt gtgaccaagc tgttcgaacg tatctacagc      300

acggatctgg gtcgcatgct gctgactagc attgttcgcg gtatcccgtt ctggggtggt      360

agcacgattg acaccgaact gaaggttatc gacactaact gcattaacgt tattcaaccg      420

gatggtagct atcgtagcga agagctgaat ctggtcatca ttggcccgag cgcagacatt      480

atccaattcg agtgcaagag ctttggtcac gaggttctga atctgacccg caatggctat      540

ggtagcaccc agtacattcg tttttcgccg gattttacct tcggctttga agagagcctg      600

gaggttgata ccaatccgtt gctgggtgcg ggcaaattcg ctaccgatcc ggctgtcacg      660

ctggcccatc aactgatcta cgcaggccac cgcctgtacg gcattgccat caacccaaac      720

cgtgtgttca aggttaatac gaatgcatac tacgagatga gcggcctgga agtcagcttc      780

gaagaactgc gcaccttcgg tggccatgac gctaaattca ttgacagctt gcaagagaat      840

gagttccgtc tgtactacta taacaaattc aaagacattg caagcacgtt gaacaaggcc      900

aaaagcatcg ttggtactac cgcgtcgttg cagtatatga agaatgtgtt taaagagaag      960

tacctgctgt ccgaggatac ctccggcaag tttagcgttg ataagctgaa gtttgacaaa     1020

ctgtacaaga tgctgaccga gatttacacc gaggacaact ttgtgaaatt cttcaaagtg     1080

ttgaatcgta aaacctatct gaattttgac aaagcggttt tcaagattaa catcgtgccg     1140

aaggtgaact acaccatcta tgacggtttt aacctgcgta acaccaacct ggcggcgaac     1200

tttaacggtc agaatacgga aatcaacaac atgaatttca cgaagttgaa gaacttcacg     1260

ggtctgttcg agttctataa gctgctgtgc gtgcgcggta tcatcaccag caaaaccaaa     1320

agcctggaca aaggctacaa caaggcgctg aatgacctgt gcattaaggt aaacaattgg     1380

gatctgttct tttcgccatc cgaagataat tttaccaacg acctgaacaa gggtgaagaa     1440

atcaccagcg atacgaatat tgaagcagcg gaagagaata tcagcctgga tctgatccag     1500

cagtactatc tgacctttaa cttcgacaat gaaccggaga acattagcat tgagaatctg     1560

agcagcgaca ttatcggtca gctggaactg atgccgaata tcgaacgttt cccgaacggc     1620

aaaaagtacg agctggacaa gtacactatg ttccattacc tgcgtgcaca ggagtttgaa     1680

cacggtaaaa gccgtatcgc gctgaccaac agcgttaacg aggccctgct gaacccgagc     1740

cgtgtctata ccttcttcag cagcgactat gttaagaaag tgaacaaagc cactgaggcc     1800

gcgatgttcc tgggctgggt ggaacagctg gtatatgact tcacggacga gacgagcgaa     1860

gtgagcacta ccgacaaaat tgctgatatt accatcatta tcccgtatat tggtccggca     1920

ctgaacattg gcaacatgct gtacaaagac gattttgtgg gtgccctgat cttctccggt     1980

gccgtgattc tgctggagtt cattccggag attgcgatcc cggtgttggg taccttcgcg     2040

ctggtgtcct acatcgcgaa taaggttctg acggttcaga ccatcgataa cgcgctgtcg     2100

aaacgtaatg aaaaatggga cgaggtttac aaatacattg ttacgaattg gctggcgaaa     2160

gtcaataccc agatcgacct gatccgtaag aaaatgaaag aggcgctgga gaatcaggcg     2220

gaggccacca aagcaattat caactaccaa tacaaccagt acacggaaga agagaagaat     2280

aacattaact tcaatatcga tgatttgagc agcaagctga atgaatctat caacaaagcg     2340

atgatcaata tcaacaagtt tttgaatcag tgtagcgttt cgtacctgat gaatagcatg     2400

attccgtatg gcgtcaaacg tctggaggac ttcgacgcca gcctgaaaga tgcgttgctg     2460

aaatacattt acgacaatcg tggtacgctg attggccaag ttgaccgctt gaaagacaaa     2520

gttaacaata ccctgagcac cgacatccca tttcaactga gcaagtatgt tgataatcaa     2580

cgtctgttga gcactttcac cgagtatatc aaaaacatca tcaatactag cattctgaac     2640

ctgcgttacg agagcaatca tctgattgat ctgagccgtt atgcaagcaa gatcaacatc     2700

ggtagcaagg tcaattttga cccgatcgat aagaaccaga tccagctgtt taatctggaa     2760

tcgagcaaaa ttgaggttat cctgaaaaac gccattgtct acaactccat gtacgagaat     2820

ttctccacca gcttctggat tcgcatcccg aaatacttca acagcattag cctgaacaac     2880

gagtatacta tcatcaactg tatggagaac aacagcggtt ggaaggtgtc tctgaactat     2940

ggtgagatca tttggacctt gcaggacacc caagagatca agcagcgcgt cgtgttcaag     3000

tactctcaaa tgatcaacat ttccgattac attaatcgtt ggatcttcgt gaccattacg     3060

aataaccgtc tgaataacag caagatttac atcaatggtc gcttgatcga tcagaaaccg     3120

attagcaacc tgggtaatat ccacgcaagc aacaacatta tgttcaaatt ggacggttgc     3180

cgcgataccc atcgttatat ctggatcaag tatttcaacc tgtttgataa agaactgaat     3240

gagaaggaga tcaaagattt gtatgacaac caatctaaca gcggcatttt gaaggacttc     3300

tggggcgatt atctgcaata cgataagccg tactatatgc tgaacctgta tgatccgaac     3360

aaatatgtgg atgtcaataa tgtgggtatt cgtggttaca tgtatttgaa gggtccgcgt     3420

ggcagcgtta tgacgaccaa catttacctg aactctagcc tgtaccgtgg tacgaaattc     3480

atcattaaga aatatgccag cggcaacaaa gataacattg tgcgtaataa cgatcgtgtc     3540

tacatcaacg tggtcgtgaa gaataaagag taccgtctgg cgaccaacgc ttcgcaggcg     3600

ggtgttgaga aaattctgag cgcgttggag atccctgatg tcggtaatct gagccaagtc     3660

gtggttatga agagcaagaa cgaccagggt atcactaaca agtgcaagat gaacctgcaa     3720

gacaacaatg gtaacgacat cggctttatt ggtttccacc agttcaacaa tattgctaaa     3780

ctggtagcga gcaattggta caatcgtcag attgagcgca gcagccgtac tttgggctgt     3840

agctgggagt ttatcccggt cgatgatggt tggggcgaac gtccgctg                  3888


<210>  2
<211>  1296
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide Sequence of rBoNT/A(0)

<400>  2

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Gln 
    210                 215                 220                 


Leu Ile Tyr Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly Tyr Asn Lys 
        435                 440                 445             


Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe 
    450                 455                 460                 


Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu 
465                 470                 475                 480 


Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu 
                485                 490                 495     


Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro 
            500                 505                 510         


Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu 
        515                 520                 525             


Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu 
    530                 535                 540                 


Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu 
545                 550                 555                 560 


His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu 
                565                 570                 575     


Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys 
            580                 585                 590         


Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu 
        595                 600                 605             


Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr 
    610                 615                 620                 


Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala 
625                 630                 635                 640 


Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu 
                645                 650                 655     


Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala 
            660                 665                 670         


Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys 
        675                 680                 685             


Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu 
    690                 695                 700                 


Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys 
705                 710                 715                 720 


Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu 
                725                 730                 735     


Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn 
            740                 745                 750         


Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp 
        755                 760                 765             


Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile 
    770                 775                 780                 


Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met 
785                 790                 795                 800 


Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys 
                805                 810                 815     


Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly 
            820                 825                 830         


Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp 
        835                 840                 845             


Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser 
    850                 855                 860                 


Thr Phe Thr Glu Tyr Ile Lys Asn Ile Ile Asn Thr Ser Ile Leu Asn 
865                 870                 875                 880 


Leu Arg Tyr Glu Ser Asn His Leu Ile Asp Leu Ser Arg Tyr Ala Ser 
                885                 890                 895     


Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp Pro Ile Asp Lys Asn 
            900                 905                 910         


Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys Ile Glu Val Ile Leu 
        915                 920                 925             


Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr Glu Asn Phe Ser Thr Ser 
    930                 935                 940                 


Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn Ser Ile Ser Leu Asn Asn 
945                 950                 955                 960 


Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn Ser Gly Trp Lys Val 
                965                 970                 975     


Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr Leu Gln Asp Thr Gln Glu 
            980                 985                 990         


Ile Lys Gln Arg Val Val Phe Lys  Tyr Ser Gln Met Ile  Asn Ile Ser 
        995                 1000                 1005             


Asp Tyr  Ile Asn Arg Trp Ile  Phe Val Thr Ile Thr  Asn Asn Arg 
    1010                 1015                 1020             


Leu Asn  Asn Ser Lys Ile Tyr  Ile Asn Gly Arg Leu  Ile Asp Gln 
    1025                 1030                 1035             


Lys Pro  Ile Ser Asn Leu Gly  Asn Ile His Ala Ser  Asn Asn Ile 
    1040                 1045                 1050             


Met Phe  Lys Leu Asp Gly Cys  Arg Asp Thr His Arg  Tyr Ile Trp 
    1055                 1060                 1065             


Ile Lys  Tyr Phe Asn Leu Phe  Asp Lys Glu Leu Asn  Glu Lys Glu 
    1070                 1075                 1080             


Ile Lys  Asp Leu Tyr Asp Asn  Gln Ser Asn Ser Gly  Ile Leu Lys 
    1085                 1090                 1095             


Asp Phe  Trp Gly Asp Tyr Leu  Gln Tyr Asp Lys Pro  Tyr Tyr Met 
    1100                 1105                 1110             


Leu Asn  Leu Tyr Asp Pro Asn  Lys Tyr Val Asp Val  Asn Asn Val 
    1115                 1120                 1125             


Gly Ile  Arg Gly Tyr Met Tyr  Leu Lys Gly Pro Arg  Gly Ser Val 
    1130                 1135                 1140             


Met Thr  Thr Asn Ile Tyr Leu  Asn Ser Ser Leu Tyr  Arg Gly Thr 
    1145                 1150                 1155             


Lys Phe  Ile Ile Lys Lys Tyr  Ala Ser Gly Asn Lys  Asp Asn Ile 
    1160                 1165                 1170             


Val Arg  Asn Asn Asp Arg Val  Tyr Ile Asn Val Val  Val Lys Asn 
    1175                 1180                 1185             


Lys Glu  Tyr Arg Leu Ala Thr  Asn Ala Ser Gln Ala  Gly Val Glu 
    1190                 1195                 1200             


Lys Ile  Leu Ser Ala Leu Glu  Ile Pro Asp Val Gly  Asn Leu Ser 
    1205                 1210                 1215             


Gln Val  Val Val Met Lys Ser  Lys Asn Asp Gln Gly  Ile Thr Asn 
    1220                 1225                 1230             


Lys Cys  Lys Met Asn Leu Gln  Asp Asn Asn Gly Asn  Asp Ile Gly 
    1235                 1240                 1245             


Phe Ile  Gly Phe His Gln Phe  Asn Asn Ile Ala Lys  Leu Val Ala 
    1250                 1255                 1260             


Ser Asn  Trp Tyr Asn Arg Gln  Ile Glu Arg Ser Ser  Arg Thr Leu 
    1265                 1270                 1275             


Gly Cys  Ser Trp Glu Phe Ile  Pro Val Asp Asp Gly  Trp Gly Glu 
    1280                 1285                 1290             


Arg Pro  Leu 
    1295     


<210>  3
<211>  2634
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide Sequence of rLHN/A

<400>  3
atggagttcg ttaacaaaca gttcaactat aaagacccag ttaacggtgt tgacattgct       60

tacatcaaaa tcccgaacgc tggccagatg cagccggtaa aggcattcaa aatccacaac      120

aaaatctggg ttatcccgga acgtgatacc tttactaacc cggaagaagg tgacctgaac      180

ccgccaccgg aagcgaaaca ggtgccggta tcttactatg actccaccta cctgtctacc      240

gataacgaaa aggacaacta cctgaaaggt gttactaaac tgttcgagcg tatttactcc      300

accgacctgg gccgtatgct gctgactagc atcgttcgcg gtatcccgtt ctggggcggt      360

tctaccatcg ataccgaact gaaagtaatc gacactaact gcatcaacgt tattcagccg      420

gacggttcct atcgttccga agaactgaac ctggtgatca tcggcccgtc tgctgatatc      480

atccagttcg agtgtaagag ctttggtcac gaagttctga acctcacccg taacggctac      540

ggttccactc agtacatccg tttctctccg gacttcacct tcggttttga agaatccctg      600

gaagtagaca cgaacccact gctgggcgct ggtaaattcg caactgatcc tgcggttacc      660

ctggctcacg aactgattca tgcaggccac cgcctgtacg gtatcgccat caatccgaac      720

cgtgtcttca aagttaacac caacgcgtat tacgagatgt ccggtctgga agttagcttc      780

gaagaactgc gtacttttgg cggtcacgac gctaaattca tcgactctct gcaagaaaac      840

gagttccgtc tgtactacta taacaagttc aaagatatcg catccaccct gaacaaagcg      900

aaatccatcg tgggtaccac tgcttctctc cagtacatga agaacgtttt taaagaaaaa      960

tacctgctca gcgaagacac ctccggcaaa ttctctgtag acaagttgaa attcgataaa     1020

ctttacaaaa tgctgactga aatttacacc gaagacaact tcgttaagtt ctttaaagtt     1080

ctgaaccgca aaacctatct gaacttcgac aaggcagtat tcaaaatcaa catcgtgccg     1140

aaagttaact acactatcta cgatggtttc aacctgcgta acaccaacct ggctgctaat     1200

tttaacggcc agaacacgga aatcaacaac atgaacttca caaaactgaa aaacttcact     1260

ggtctgttcg agttttacaa gctgctgtgc gtcgacggca tcattacctc caaaactaaa     1320

tctgacgatg acgataaaaa caaagcgctg aacctgcagt gtatcaaggt taacaactgg     1380

gatttattct tcagcccgag tgaagacaac ttcaccaacg acctgaacaa aggtgaagaa     1440

atcacctcag atactaacat cgaagcagcc gaagaaaaca tctcgctgga cctgatccag     1500

cagtactacc tgacctttaa tttcgacaac gagccggaaa acatttctat cgaaaacctg     1560

agctctgata tcatcggcca gctggaactg atgccgaaca tcgaacgttt cccaaacggt     1620

aaaaagtacg agctggacaa atataccatg ttccactacc tgcgcgcgca ggaatttgaa     1680

cacggcaaat cccgtatcgc actgactaac tccgttaacg aagctctgct caacccgtcc     1740

cgtgtataca ccttcttctc tagcgactac gtgaaaaagg tcaacaaagc gactgaagct     1800

gcaatgttct tgggttgggt tgaacagctt gtttatgatt ttaccgacga gacgtccgaa     1860

gtatctacta ccgacaaaat tgcggatatc actatcatca tcccgtacat cggtccggct     1920

ctgaacattg gcaacatgct gtacaaagac gacttcgttg gcgcactgat cttctccggt     1980

gcggtgatcc tgctggagtt catcccggaa atcgccatcc cggtactggg cacctttgct     2040

ctggtttctt acattgcaaa caaggttctg actgtacaaa ccatcgacaa cgcgctgagc     2100

aaacgtaacg aaaaatggga tgaagtttac aaatatatcg tgaccaactg gctggctaag     2160

gttaatactc agatcgacct catccgcaaa aaaatgaaag aagcactgga aaaccaggcg     2220

gaagctacca aggcaatcat taactaccag tacaaccagt acaccgagga agaaaaaaac     2280

aacatcaact tcaacatcga cgatctgtcc tctaaactga acgaatccat caacaaagct     2340

atgatcaaca tcaacaagtt cctgaaccag tgctctgtaa gctatctgat gaactccatg     2400

atcccgtacg gtgttaaacg tctggaggac ttcgatgcgt ctctgaaaga cgccctgctg     2460

aaatacattt acgacaaccg tggcactctg atcggtcagg ttgatcgtct gaaggacaaa     2520

gtgaacaata ccttatcgac cgacatccct tttcagctca gtaaatatgt cgataaccaa     2580

cgccttttgt ccactctaga agcacaccat catcaccacc atcaccatca ccat           2634


<210>  4
<211>  878
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide Sequence of rLHN/A

<400>  4

Met Glu Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Asp 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Asp Asp Asp Asp Lys Asn Lys 
        435                 440                 445             


Ala Leu Asn Leu Gln Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe 
    450                 455                 460                 


Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu 
465                 470                 475                 480 


Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu 
                485                 490                 495     


Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro 
            500                 505                 510         


Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu 
        515                 520                 525             


Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu 
    530                 535                 540                 


Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu 
545                 550                 555                 560 


His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu 
                565                 570                 575     


Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys 
            580                 585                 590         


Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu 
        595                 600                 605             


Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr 
    610                 615                 620                 


Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala 
625                 630                 635                 640 


Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu 
                645                 650                 655     


Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala 
            660                 665                 670         


Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys 
        675                 680                 685             


Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu 
    690                 695                 700                 


Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys 
705                 710                 715                 720 


Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu 
                725                 730                 735     


Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn 
            740                 745                 750         


Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp 
        755                 760                 765             


Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile 
    770                 775                 780                 


Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met 
785                 790                 795                 800 


Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys 
                805                 810                 815     


Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly 
            820                 825                 830         


Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp 
        835                 840                 845             


Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser 
    850                 855                 860                 


Thr Leu Glu Ala His His His His His His His His His His 
865                 870                 875             


<210>  5
<211>  1329
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide Sequence of rL/A

<400>  5
atgccattcg tcaacaagca attcaactac aaagacccag tcaacggcgt cgacatcgca       60

tacatcaaga ttccgaacgc cggtcaaatg cagccggtta aggcttttaa gatccacaac      120

aagatttggg ttatcccgga gcgtgacacc ttcacgaacc cggaagaagg cgatctgaac      180

ccgccaccgg aagcgaagca agtccctgtc agctactacg attcgacgta cctgagcacg      240

gataacgaaa aagataacta cctgaaaggt gtgaccaagc tgttcgaacg tatctacagc      300

acggatctgg gtcgcatgct gctgactagc attgttcgcg gtatcccgtt ctggggtggt      360

agcacgattg acaccgaact gaaggttatc gacactaact gcattaacgt tattcaaccg      420

gatggtagct atcgtagcga agagctgaat ctggtcatca ttggcccgag cgcagacatt      480

atccaattcg agtgcaagag ctttggtcac gaggttctga atctgacccg caatggctat      540

ggtagcaccc agtacattcg tttttcgccg gattttacct tcggctttga agagagcctg      600

gaggttgata ccaatccgtt gctgggtgcg ggcaaattcg ctaccgatcc ggctgtcacg      660

ctggcccatg aactgatcca cgcaggccac cgcctgtacg gcattgccat caacccaaac      720

cgtgtgttca aggttaatac gaatgcatac tacgagatga gcggcctgga agtcagcttc      780

gaagaactgc gcaccttcgg tggccatgac gctaaattca ttgacagctt gcaagagaat      840

gagttccgtc tgtactacta taacaaattc aaagacattg caagcacgtt gaacaaggcc      900

aaaagcatcg ttggtactac cgcgtcgttg cagtatatga agaatgtgtt taaagagaag      960

tacctgctgt ccgaggatac ctccggcaag tttagcgttg ataagctgaa gtttgacaaa     1020

ctgtacaaga tgctgaccga gatttacacc gaggacaact ttgtgaaatt cttcaaagtg     1080

ttgaatcgta aaacctatct gaattttgac aaagcggttt tcaagattaa catcgtgccg     1140

aaggtgaact acaccatcta tgacggtttt aacctgcgta acaccaacct ggcggcgaac     1200

tttaacggtc agaatacgga aatcaacaac atgaatttca cgaagttgaa gaacttcacg     1260

ggtctgttcg agttctataa gctgctgggt ctagaagcac accatcatca ccaccatcac     1320

catcaccat                                                             1329


<210>  6
<211>  443
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide Sequence of rL/A

<400>  6

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Gly Leu Glu 
            420                 425                 430         


Ala His His His His His His His His His His 
        435                 440             


<210>  7
<211>  1299
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide Sequence of rHC/A

<400>  7
atgcatcatc accatcacca caaaaacatc atcaatacta gcattctgaa cctgcgttac       60

gagagcaatc atctgattga tctgagccgt tatgcaagca agatcaacat cggtagcaag      120

gtcaattttg acccgatcga taagaaccag atccagctgt ttaatctgga atcgagcaaa      180

attgaggtta tcctgaaaaa cgccattgtc tacaactcca tgtacgagaa tttctccacc      240

agcttctgga ttcgcatccc gaaatacttc aacagcatta gcctgaacaa cgagtatact      300

atcatcaact gtatggagaa caacagcggt tggaaggtgt ctctgaacta tggtgagatc      360

atttggacct tgcaggacac ccaagagatc aagcagcgcg tcgtgttcaa gtactctcaa      420

atgatcaaca tttccgatta cattaatcgt tggatcttcg tgaccattac gaataaccgt      480

ctgaataaca gcaagattta catcaatggt cgcttgatcg atcagaaacc gattagcaac      540

ctgggtaata tccacgcaag caacaacatt atgttcaaat tggacggttg ccgcgatacc      600

catcgttata tctggatcaa gtatttcaac ctgtttgata aagaactgaa tgagaaggag      660

atcaaagatt tgtatgacaa ccaatctaac agcggcattt tgaaggactt ctggggcgat      720

tatctgcaat acgataagcc gtactatatg ctgaacctgt atgatccgaa caaatatgtg      780

gatgtcaata atgtgggtat tcgtggttac atgtatttga agggtccgcg tggcagcgtt      840

atgacgacca acatttacct gaactctagc ctgtaccgtg gtacgaaatt catcattaag      900

aaatatgcca gcggcaacaa agataacatt gtgcgtaata acgatcgtgt ctacatcaac      960

gtggtcgtga agaataaaga gtaccgtctg gcgaccaacg cttcgcaggc gggtgttgag     1020

aaaattctga gcgcgttgga gatccctgat gtcggtaatc tgagccaagt cgtggttatg     1080

aagagcaaga acgaccaggg tatcactaac aagtgcaaga tgaacctgca agacaacaat     1140

ggtaacgaca tcggctttat tggtttccac cagttcaaca atattgctaa actggtagcg     1200

agcaattggt acaatcgtca gattgagcgc agcagccgta ctttgggctg tagctgggag     1260

tttatcccgg tcgatgatgg ttggggcgaa cgtccgctg                            1299


<210>  8
<211>  433
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide Sequence of rHC/A

<400>  8

Met His His His His His His Lys Asn Ile Ile Asn Thr Ser Ile Leu 
1               5                   10                  15      


Asn Leu Arg Tyr Glu Ser Asn His Leu Ile Asp Leu Ser Arg Tyr Ala 
            20                  25                  30          


Ser Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp Pro Ile Asp Lys 
        35                  40                  45              


Asn Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys Ile Glu Val Ile 
    50                  55                  60                  


Leu Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr Glu Asn Phe Ser Thr 
65                  70                  75                  80  


Ser Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn Ser Ile Ser Leu Asn 
                85                  90                  95      


Asn Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn Ser Gly Trp Lys 
            100                 105                 110         


Val Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr Leu Gln Asp Thr Gln 
        115                 120                 125             


Glu Ile Lys Gln Arg Val Val Phe Lys Tyr Ser Gln Met Ile Asn Ile 
    130                 135                 140                 


Ser Asp Tyr Ile Asn Arg Trp Ile Phe Val Thr Ile Thr Asn Asn Arg 
145                 150                 155                 160 


Leu Asn Asn Ser Lys Ile Tyr Ile Asn Gly Arg Leu Ile Asp Gln Lys 
                165                 170                 175     


Pro Ile Ser Asn Leu Gly Asn Ile His Ala Ser Asn Asn Ile Met Phe 
            180                 185                 190         


Lys Leu Asp Gly Cys Arg Asp Thr His Arg Tyr Ile Trp Ile Lys Tyr 
        195                 200                 205             


Phe Asn Leu Phe Asp Lys Glu Leu Asn Glu Lys Glu Ile Lys Asp Leu 
    210                 215                 220                 


Tyr Asp Asn Gln Ser Asn Ser Gly Ile Leu Lys Asp Phe Trp Gly Asp 
225                 230                 235                 240 


Tyr Leu Gln Tyr Asp Lys Pro Tyr Tyr Met Leu Asn Leu Tyr Asp Pro 
                245                 250                 255     


Asn Lys Tyr Val Asp Val Asn Asn Val Gly Ile Arg Gly Tyr Met Tyr 
            260                 265                 270         


Leu Lys Gly Pro Arg Gly Ser Val Met Thr Thr Asn Ile Tyr Leu Asn 
        275                 280                 285             


Ser Ser Leu Tyr Arg Gly Thr Lys Phe Ile Ile Lys Lys Tyr Ala Ser 
    290                 295                 300                 


Gly Asn Lys Asp Asn Ile Val Arg Asn Asn Asp Arg Val Tyr Ile Asn 
305                 310                 315                 320 


Val Val Val Lys Asn Lys Glu Tyr Arg Leu Ala Thr Asn Ala Ser Gln 
                325                 330                 335     


Ala Gly Val Glu Lys Ile Leu Ser Ala Leu Glu Ile Pro Asp Val Gly 
            340                 345                 350         


Asn Leu Ser Gln Val Val Val Met Lys Ser Lys Asn Asp Gln Gly Ile 
        355                 360                 365             


Thr Asn Lys Cys Lys Met Asn Leu Gln Asp Asn Asn Gly Asn Asp Ile 
    370                 375                 380                 


Gly Phe Ile Gly Phe His Gln Phe Asn Asn Ile Ala Lys Leu Val Ala 
385                 390                 395                 400 


Ser Asn Trp Tyr Asn Arg Gln Ile Glu Arg Ser Ser Arg Thr Leu Gly 
                405                 410                 415     


Cys Ser Trp Glu Phe Ile Pro Val Asp Asp Gly Trp Gly Glu Arg Pro 
            420                 425                 430         


Leu 
    


<210>  9
<211>  3873
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide Sequence of rBoNT/B(0)

<400>  9
atgccggtga cgattaacaa cttcaactac aacgacccga ttgacaacaa caacattatc       60

atgatggaac cgccgtttgc acgcggcacg ggccgttatt acaaagcgtt taaaatcacc      120

gatcgtattt ggattatccc ggaacgctac acgtttggtt ataaaccgga agacttcaac      180

aaaagctctg gcatcttcaa ccgtgatgtt tgcgaatact acgatccgga ctacctgaac      240

accaacgata agaaaaacat ttttctgcaa acgatgatca aactgttcaa tcgcattaaa      300

agcaaaccgc tgggtgaaaa actgctggaa atgattatca atggcattcc gtatctgggt      360

gatcgtcgcg tgccgctgga agaatttaac accaatatcg cgagtgttac ggtcaacaaa      420

ctgatttcca atccgggtga agtcgaacgt aaaaaaggca tcttcgccaa cctgatcatc      480

ttcggcccgg gtccggtgct gaacgaaaat gaaaccattg atatcggtat tcagaaccat      540

tttgcctcac gcgaaggctt cggcggtatt atgcaaatga aattttgccc ggaatatgtg      600

tcggttttca acaatgttca ggaaaacaaa ggtgcaagca tctttaatcg tcgcggctat      660

ttctctgatc cggctctgat cctgatgcac caactgattt atgtgctgca cggcctgtat      720

ggtatcaaag tggatgacct gccgatcgtt ccgaacgaga aaaaattttt catgcagagc      780

accgacgcaa ttcaagctga agaactgtat acgtttggcg gtcaggaccc gtctattatc      840

accccgagca ccgacaaaag catctacgat aaagtgctgc aaaactttcg tggcattgtt      900

gaccgcctga ataaagtcct ggtgtgtatc tctgatccga acatcaacat caacatctac      960

aaaaacaaat tcaaagacaa atacaaattc gttgaagatt ctgaaggcaa atatagtatt     1020

gacgtcgaat cctttgataa actgtacaaa agtctgatgt tcggtttcac cgaaacgaac     1080

atcgcggaaa actacaaaat caaaacccgc gcctcctatt tcagcgactc tctgccgccg     1140

gttaaaatca aaaatctgct ggataacgaa atttatacga tcgaagaagg tttcaacatc     1200

agcgataaag acatggaaaa agaataccgt ggccagaata aagcaatcaa caaacaggcg     1260

tatgaagaaa ttagtaaaga acatctggcg gtctacaaaa ttcagatgtg caaatccgtg     1320

aaagccccgg gtatttgtat cgatgttgac aatgaagacc tgtttttcat cgccgataaa     1380

aacagttttt ccgatgacct gtcaaaaaat gaacgcatcg aatacaacac ccaatcgaac     1440

tacatcgaaa acgatttccc gatcaacgaa ctgattctgg atacggacct gattagtaaa     1500

atcgaactgc cgtcagaaaa caccgaatcg ctgacggact ttaatgttga tgtcccggtg     1560

tatgaaaaac agccggcaat taagaaaatt tttaccgatg aaaacacgat cttccagtac     1620

ctgtacagcc aaacctttcc gctggacatt cgcgatatct ctctgacgag ttcctttgat     1680

gacgcactgc tgttcagcaa caaagtgtac tcctttttct caatggatta catcaaaacc     1740

gctaacaaag tggttgaagc gggcctgttt gccggttggg tgaaacagat cgttaacgat     1800

ttcgtcatcg aagccaacaa aagtaacacg atggataaaa ttgctgatat ctccctgatt     1860

gtcccgtata ttggcctggc actgaatgtg ggtaacgaaa cggcgaaagg caattttgaa     1920

aacgccttcg aaattgcagg cgcttcaatc ctgctggaat ttattccgga actgctgatc     1980

ccggtcgtgg gtgcgttcct gctggaatct tacatcgaca acaaaaacaa aatcatcaaa     2040

accattgata acgcgctgac gaaacgtaac gaaaaatggt cagatatgta cggcctgatt     2100

gttgcccagt ggctgagcac cgtcaacacg caattttaca ccatcaaaga aggtatgtac     2160

aaagcgctga attatcaggc gcaagccctg gaagaaatca tcaaataccg ctacaacatc     2220

tacagcgaaa aagaaaaatc taacatcaac atcgacttta atgatatcaa cagcaaactg     2280

aacgaaggta tcaaccaggc aatcgataac atcaacaact tcatcaacgg ctgctcagtg     2340

tcgtatctga tgaagaaaat gatcccgctg gctgttgaaa aactgctgga ttttgacaac     2400

accctgaaga aaaacctgct gaactacatc gatgaaaaca aactgtacct gatcggctca     2460

gccgaatacg aaaaatcgaa agtgaacaaa tacctgaaaa ccatcatgcc gtttgacctg     2520

agtatttaca ccaacgatac gatcctgatc gaaatgttca acaaatacaa ctccgaaatt     2580

ctgaacaata ttatcctgaa cctgcgttac aaagacaaca atctgatcga tctgagcggc     2640

tatggtgcaa aagttgaagt ctacgacggt gtcgaactga acgataaaaa ccagttcaaa     2700

ctgacctcat cggctaactc aaaaattcgt gtgacgcaga accaaaacat catcttcaac     2760

tcggtctttc tggacttcag cgtgtctttc tggattcgca tcccgaaata taaaaatgat     2820

ggcatccaga actacatcca taacgaatac accatcatca actgtatgaa aaacaacagt     2880

ggttggaaaa tttccatccg tggcaaccgc attatctgga ccctgattga tatcaatggt     2940

aaaacgaaaa gcgtgttttt cgaatacaac atccgtgaag atatctctga atacatcaat     3000

cgctggtttt tcgtgaccat tacgaacaat ctgaacaatg cgaaaatcta tatcaacggc     3060

aaactggaaa gtaataccga catcaaagat attcgtgaag ttatcgccaa cggtgaaatc     3120

atcttcaaac tggatggcga catcgatcgc acccagttca tttggatgaa atacttctcc     3180

atcttcaaca cggaactgag tcagtccaat atcgaagaac gctacaaaat ccaatcatac     3240

tcggaatacc tgaaagattt ctggggtaac ccgctgatgt acaacaaaga atactacatg     3300

ttcaacgcgg gcaacaaaaa ctcatacatc aaactgaaaa aagattcgcc ggtgggtgaa     3360

atcctgaccc gtagcaaata caaccagaac tctaaataca tcaactatcg cgatctgtac     3420

attggcgaaa aatttattat ccgtcgcaaa agcaactctc agagtattaa tgatgacatc     3480

gtgcgtaaag aagactacat ctatctggat ttctttaatc tgaaccaaga atggcgcgtt     3540

tatacctaca aatacttcaa aaaagaagaa gagaaactgt tcctggcccc gattagcgac     3600

agcgatgaat tttacaacac catccagatc aaagaatacg atgaacagcc gacgtatagt     3660

tgccaactgc tgttcaaaaa agacgaagaa tccaccgatg aaattggcct gattggtatc     3720

caccgtttct atgaaagcgg tatcgttttc gaagaataca aagattactt ctgtatctct     3780

aaatggtatc tgaaagaagt caaacgcaaa ccgtacaacc tgaaactggg ctgcaactgg     3840

caatttatcc cgaaagacga aggctggacc gaa                                  3873


<210>  10
<211>  1291
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide Sequence of rBoNT/B(0)

<400>  10

Met Pro Val Thr Ile Asn Asn Phe Asn Tyr Asn Asp Pro Ile Asp Asn 
1               5                   10                  15      


Asn Asn Ile Ile Met Met Glu Pro Pro Phe Ala Arg Gly Thr Gly Arg 
            20                  25                  30          


Tyr Tyr Lys Ala Phe Lys Ile Thr Asp Arg Ile Trp Ile Ile Pro Glu 
        35                  40                  45              


Arg Tyr Thr Phe Gly Tyr Lys Pro Glu Asp Phe Asn Lys Ser Ser Gly 
    50                  55                  60                  


Ile Phe Asn Arg Asp Val Cys Glu Tyr Tyr Asp Pro Asp Tyr Leu Asn 
65                  70                  75                  80  


Thr Asn Asp Lys Lys Asn Ile Phe Leu Gln Thr Met Ile Lys Leu Phe 
                85                  90                  95      


Asn Arg Ile Lys Ser Lys Pro Leu Gly Glu Lys Leu Leu Glu Met Ile 
            100                 105                 110         


Ile Asn Gly Ile Pro Tyr Leu Gly Asp Arg Arg Val Pro Leu Glu Glu 
        115                 120                 125             


Phe Asn Thr Asn Ile Ala Ser Val Thr Val Asn Lys Leu Ile Ser Asn 
    130                 135                 140                 


Pro Gly Glu Val Glu Arg Lys Lys Gly Ile Phe Ala Asn Leu Ile Ile 
145                 150                 155                 160 


Phe Gly Pro Gly Pro Val Leu Asn Glu Asn Glu Thr Ile Asp Ile Gly 
                165                 170                 175     


Ile Gln Asn His Phe Ala Ser Arg Glu Gly Phe Gly Gly Ile Met Gln 
            180                 185                 190         


Met Lys Phe Cys Pro Glu Tyr Val Ser Val Phe Asn Asn Val Gln Glu 
        195                 200                 205             


Asn Lys Gly Ala Ser Ile Phe Asn Arg Arg Gly Tyr Phe Ser Asp Pro 
    210                 215                 220                 


Ala Leu Ile Leu Met His Gln Leu Ile Tyr Val Leu His Gly Leu Tyr 
225                 230                 235                 240 


Gly Ile Lys Val Asp Asp Leu Pro Ile Val Pro Asn Glu Lys Lys Phe 
                245                 250                 255     


Phe Met Gln Ser Thr Asp Ala Ile Gln Ala Glu Glu Leu Tyr Thr Phe 
            260                 265                 270         


Gly Gly Gln Asp Pro Ser Ile Ile Thr Pro Ser Thr Asp Lys Ser Ile 
        275                 280                 285             


Tyr Asp Lys Val Leu Gln Asn Phe Arg Gly Ile Val Asp Arg Leu Asn 
    290                 295                 300                 


Lys Val Leu Val Cys Ile Ser Asp Pro Asn Ile Asn Ile Asn Ile Tyr 
305                 310                 315                 320 


Lys Asn Lys Phe Lys Asp Lys Tyr Lys Phe Val Glu Asp Ser Glu Gly 
                325                 330                 335     


Lys Tyr Ser Ile Asp Val Glu Ser Phe Asp Lys Leu Tyr Lys Ser Leu 
            340                 345                 350         


Met Phe Gly Phe Thr Glu Thr Asn Ile Ala Glu Asn Tyr Lys Ile Lys 
        355                 360                 365             


Thr Arg Ala Ser Tyr Phe Ser Asp Ser Leu Pro Pro Val Lys Ile Lys 
    370                 375                 380                 


Asn Leu Leu Asp Asn Glu Ile Tyr Thr Ile Glu Glu Gly Phe Asn Ile 
385                 390                 395                 400 


Ser Asp Lys Asp Met Glu Lys Glu Tyr Arg Gly Gln Asn Lys Ala Ile 
                405                 410                 415     


Asn Lys Gln Ala Tyr Glu Glu Ile Ser Lys Glu His Leu Ala Val Tyr 
            420                 425                 430         


Lys Ile Gln Met Cys Lys Ser Val Lys Ala Pro Gly Ile Cys Ile Asp 
        435                 440                 445             


Val Asp Asn Glu Asp Leu Phe Phe Ile Ala Asp Lys Asn Ser Phe Ser 
    450                 455                 460                 


Asp Asp Leu Ser Lys Asn Glu Arg Ile Glu Tyr Asn Thr Gln Ser Asn 
465                 470                 475                 480 


Tyr Ile Glu Asn Asp Phe Pro Ile Asn Glu Leu Ile Leu Asp Thr Asp 
                485                 490                 495     


Leu Ile Ser Lys Ile Glu Leu Pro Ser Glu Asn Thr Glu Ser Leu Thr 
            500                 505                 510         


Asp Phe Asn Val Asp Val Pro Val Tyr Glu Lys Gln Pro Ala Ile Lys 
        515                 520                 525             


Lys Ile Phe Thr Asp Glu Asn Thr Ile Phe Gln Tyr Leu Tyr Ser Gln 
    530                 535                 540                 


Thr Phe Pro Leu Asp Ile Arg Asp Ile Ser Leu Thr Ser Ser Phe Asp 
545                 550                 555                 560 


Asp Ala Leu Leu Phe Ser Asn Lys Val Tyr Ser Phe Phe Ser Met Asp 
                565                 570                 575     


Tyr Ile Lys Thr Ala Asn Lys Val Val Glu Ala Gly Leu Phe Ala Gly 
            580                 585                 590         


Trp Val Lys Gln Ile Val Asn Asp Phe Val Ile Glu Ala Asn Lys Ser 
        595                 600                 605             


Asn Thr Met Asp Lys Ile Ala Asp Ile Ser Leu Ile Val Pro Tyr Ile 
    610                 615                 620                 


Gly Leu Ala Leu Asn Val Gly Asn Glu Thr Ala Lys Gly Asn Phe Glu 
625                 630                 635                 640 


Asn Ala Phe Glu Ile Ala Gly Ala Ser Ile Leu Leu Glu Phe Ile Pro 
                645                 650                 655     


Glu Leu Leu Ile Pro Val Val Gly Ala Phe Leu Leu Glu Ser Tyr Ile 
            660                 665                 670         


Asp Asn Lys Asn Lys Ile Ile Lys Thr Ile Asp Asn Ala Leu Thr Lys 
        675                 680                 685             


Arg Asn Glu Lys Trp Ser Asp Met Tyr Gly Leu Ile Val Ala Gln Trp 
    690                 695                 700                 


Leu Ser Thr Val Asn Thr Gln Phe Tyr Thr Ile Lys Glu Gly Met Tyr 
705                 710                 715                 720 


Lys Ala Leu Asn Tyr Gln Ala Gln Ala Leu Glu Glu Ile Ile Lys Tyr 
                725                 730                 735     


Arg Tyr Asn Ile Tyr Ser Glu Lys Glu Lys Ser Asn Ile Asn Ile Asp 
            740                 745                 750         


Phe Asn Asp Ile Asn Ser Lys Leu Asn Glu Gly Ile Asn Gln Ala Ile 
        755                 760                 765             


Asp Asn Ile Asn Asn Phe Ile Asn Gly Cys Ser Val Ser Tyr Leu Met 
    770                 775                 780                 


Lys Lys Met Ile Pro Leu Ala Val Glu Lys Leu Leu Asp Phe Asp Asn 
785                 790                 795                 800 


Thr Leu Lys Lys Asn Leu Leu Asn Tyr Ile Asp Glu Asn Lys Leu Tyr 
                805                 810                 815     


Leu Ile Gly Ser Ala Glu Tyr Glu Lys Ser Lys Val Asn Lys Tyr Leu 
            820                 825                 830         


Lys Thr Ile Met Pro Phe Asp Leu Ser Ile Tyr Thr Asn Asp Thr Ile 
        835                 840                 845             


Leu Ile Glu Met Phe Asn Lys Tyr Asn Ser Glu Ile Leu Asn Asn Ile 
    850                 855                 860                 


Ile Leu Asn Leu Arg Tyr Lys Asp Asn Asn Leu Ile Asp Leu Ser Gly 
865                 870                 875                 880 


Tyr Gly Ala Lys Val Glu Val Tyr Asp Gly Val Glu Leu Asn Asp Lys 
                885                 890                 895     


Asn Gln Phe Lys Leu Thr Ser Ser Ala Asn Ser Lys Ile Arg Val Thr 
            900                 905                 910         


Gln Asn Gln Asn Ile Ile Phe Asn Ser Val Phe Leu Asp Phe Ser Val 
        915                 920                 925             


Ser Phe Trp Ile Arg Ile Pro Lys Tyr Lys Asn Asp Gly Ile Gln Asn 
    930                 935                 940                 


Tyr Ile His Asn Glu Tyr Thr Ile Ile Asn Cys Met Lys Asn Asn Ser 
945                 950                 955                 960 


Gly Trp Lys Ile Ser Ile Arg Gly Asn Arg Ile Ile Trp Thr Leu Ile 
                965                 970                 975     


Asp Ile Asn Gly Lys Thr Lys Ser Val Phe Phe Glu Tyr Asn Ile Arg 
            980                 985                 990         


Glu Asp Ile Ser Glu Tyr Ile Asn  Arg Trp Phe Phe Val  Thr Ile Thr 
        995                 1000                 1005             


Asn Asn  Leu Asn Asn Ala Lys  Ile Tyr Ile Asn Gly  Lys Leu Glu 
    1010                 1015                 1020             


Ser Asn  Thr Asp Ile Lys Asp  Ile Arg Glu Val Ile  Ala Asn Gly 
    1025                 1030                 1035             


Glu Ile  Ile Phe Lys Leu Asp  Gly Asp Ile Asp Arg  Thr Gln Phe 
    1040                 1045                 1050             


Ile Trp  Met Lys Tyr Phe Ser  Ile Phe Asn Thr Glu  Leu Ser Gln 
    1055                 1060                 1065             


Ser Asn  Ile Glu Glu Arg Tyr  Lys Ile Gln Ser Tyr  Ser Glu Tyr 
    1070                 1075                 1080             


Leu Lys  Asp Phe Trp Gly Asn  Pro Leu Met Tyr Asn  Lys Glu Tyr 
    1085                 1090                 1095             


Tyr Met  Phe Asn Ala Gly Asn  Lys Asn Ser Tyr Ile  Lys Leu Lys 
    1100                 1105                 1110             


Lys Asp  Ser Pro Val Gly Glu  Ile Leu Thr Arg Ser  Lys Tyr Asn 
    1115                 1120                 1125             


Gln Asn  Ser Lys Tyr Ile Asn  Tyr Arg Asp Leu Tyr  Ile Gly Glu 
    1130                 1135                 1140             


Lys Phe  Ile Ile Arg Arg Lys  Ser Asn Ser Gln Ser  Ile Asn Asp 
    1145                 1150                 1155             


Asp Ile  Val Arg Lys Glu Asp  Tyr Ile Tyr Leu Asp  Phe Phe Asn 
    1160                 1165                 1170             


Leu Asn  Gln Glu Trp Arg Val  Tyr Thr Tyr Lys Tyr  Phe Lys Lys 
    1175                 1180                 1185             


Glu Glu  Glu Lys Leu Phe Leu  Ala Pro Ile Ser Asp  Ser Asp Glu 
    1190                 1195                 1200             


Phe Tyr  Asn Thr Ile Gln Ile  Lys Glu Tyr Asp Glu  Gln Pro Thr 
    1205                 1210                 1215             


Tyr Ser  Cys Gln Leu Leu Phe  Lys Lys Asp Glu Glu  Ser Thr Asp 
    1220                 1225                 1230             


Glu Ile  Gly Leu Ile Gly Ile  His Arg Phe Tyr Glu  Ser Gly Ile 
    1235                 1240                 1245             


Val Phe  Glu Glu Tyr Lys Asp  Tyr Phe Cys Ile Ser  Lys Trp Tyr 
    1250                 1255                 1260             


Leu Lys  Glu Val Lys Arg Lys  Pro Tyr Asn Leu Lys  Leu Gly Cys 
    1265                 1270                 1275             


Asn Trp  Gln Phe Ile Pro Lys  Asp Glu Gly Trp Thr  Glu 
    1280                 1285                 1290     


<210>  11
<211>  3873
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide Sequence of rBoNT/C(0)

<400>  11
atgccgatca cgattaataa tttcaactat agcgatccgg tggacaataa gaatattctg       60

tatctggata ctcatctgaa tacgctggct aacgaaccgg agaaagcgtt ccgcatcaca      120

ggcaacatct gggttattcc cgatcgcttt tcacgcaaca gcaaccctaa tctgaacaaa      180

cctcctcgtg tcaccagtcc taaatccggt tattacgacc caaactatct gagtacggat      240

agcgataaag atccctttct gaaagagatc attaagctgt tcaaacgcat taactctcgc      300

gaaattgggg aagagctgat ctatcggctt tcgacagata tcccgttccc aggtaacaat      360

aataccccga ttaatacttt cgactttgat gttgatttca attctgtgga tgtgaaaacg      420

cgtcaaggca ataattgggt gaaaactggt agcattaacc cgagtgtaat tatcacaggt      480

ccccgtgaga acatcatcga cccggaaacc tctaccttca agctgacgaa caacacgttt      540

gctgcacagg aagggtttgg tgccctgtca atcatttcca tctcaccgcg tttcatgtta      600

acctactcca atgccacaaa tgatgttggc gaaggacgtt ttagcaaatc agaattttgc      660

atggacccaa ttctcattct gatgggcacg ctgaacaatg cgatgcacaa cttgtatggc      720

attgctattc caaacgatca aaccattagc tccgttacca gtaatatctt ctatagccag      780

tataatgtca aattggagta tgccgaaatt tacgcctttg gaggcccgac cattgacctg      840

attccgaaat ctgcacgcaa atacttcgaa gaaaaggcgt tagattacta tcgcagcatc      900

gcgaaacgcc tgaactcgat taccacggcc aatccgtcgt cgttcaacaa atacattggt      960

gaatataaac agaaactgat tcgcaaatat cggtttgtcg tagaaagctc tggtgaagtg     1020

actgtaaacc gcaacaaatt tgtcgaactc tacaacgagt tgacccaaat ctttaccgag     1080

tttaactacg caaagatcta taacgtacag aaccgcaaga tttatcttag caatgtatac     1140

acaccggtta ctgcgaacat cttagacgac aatgtgtatg atattcagaa tggctttaac     1200

atcccgaaat caaatctgaa cgttctgttt atgggccaga acctgagtcg taatccagca     1260

ctgcgtaaag tgaacccgga aaatatgctc tacttgttta ccaaattttg ccacaaagcg     1320

attgatggcc gctctctcta taacaaaacg ctggattgtc gtgagttact tgtgaagaac     1380

actgatttac cgttcattgg ggatatctcc gacgtgaaaa ccgatatctt cctgcgcaaa     1440

gacattaatg aagaaacgga agtcatctat taccccgaca atgtgagcgt tgatcaggtc     1500

attttatcga agaacacctc cgaacatggt cagttggatt tgctgtaccc tagcattgac     1560

tcggagagtg aaatccttcc gggcgaaaat caagtgtttt acgacaaccg tacccaaaat     1620

gttgattatt tgaattctta ttactacctg gaatctcaga aattgagcga caatgtggaa     1680

gatttcacgt tcacacgctc cattgaggaa gcgctggata atagcgcgaa agtgtatacg     1740

tatttcccta ccttggcgaa taaagtaaat gctggtgtcc agggaggctt atttctgatg     1800

tgggcgaatg atgtggtaga agattttacg accaatattt tgcgtaagga caccttagat     1860

aaaattagcg atgttagcgc catcatcccc tatattggcc cagcactgaa tatctcgaac     1920

tctgtgcgtc gcggaaactt caccgaagca tttgcggtga ccggggttac tattctgttg     1980

gaagcctttc cggagtttac tattccggcg ctgggtgcgt ttgtgattta ttcgaaagta     2040

caagaacgca atgaaattat caaaaccatc gataattgcc tggaacaacg cattaaacgc     2100

tggaaggatt cttatgaatg gatgatgggc acctggttat cccgtattat cacacagttt     2160

aacaacatct cgtatcagat gtacgattca ctgaactacc aagcaggggc gatcaaagcc     2220

aagatcgact tagaatacaa gaaatattca ggtagcgata aagagaatat taaaagccag     2280

gttgaaaacc tgaagaactc tctggatgtc aaaatttcag aggctatgaa caacattaac     2340

aaatttatcc gcgaatgtag cgtcacgtat ctgtttaaaa acatgctccc gaaagtgatt     2400

gatgagctca acgagtttga tcgcaacaca aaggccaaac tgattaacct gattgatagt     2460

cacaatatta ttttagtcgg tgaagttgac aagctgaagg ctaaggtcaa taacagcttt     2520

cagaacacta ttccgtttaa tattttctcc tatacgaaca atagtctgct gaaagacatt     2580

atcaacgaat acttcaacaa tattaatgac agcaaaattc tgagcctgca gaatcgtaag     2640

aatacgctgg tagataccag tggatataat gcggaagtct cagaagaggg tgatgtacag     2700

ctgaacccga tctttccgtt cgactttaaa ctggggtcta gtggtgaaga tcgcggtaaa     2760

gtgatcgtta cccaaaacga gaacattgtg tataacagca tgtacgagag tttctcaatt     2820

tctttctgga ttcgcatcaa taaatgggtt tctaatttgc ctggctatac catcattgat     2880

agcgtcaaaa acaactcggg ctggtcgatt ggcattatta gcaactttct ggtgtttacc     2940

ctgaaacaga atgaggattc ggaacagagc attaacttct cctacgacat cagcaacaat     3000

gcaccagggt ataacaaatg gttcttcgta acggtgacga acaatatgat gggcaatatg     3060

aaaatctaca ttaacgggaa acttatcgac accattaaag tgaaagagct tactgggatc     3120

aattttagta aaaccattac ctttgagatc aacaaaattc cggacacggg tctgattacc     3180

tccgattcgg ataatatcaa tatgtggatt cgcgactttt atatcttcgc caaagaactt     3240

gatggcaaag atatcaacat tttgtttaat tccctgcagt ataccaatgt cgttaaggac     3300

tattggggca atgatctccg ctacaataaa gaatactaca tggttaacat cgactatctc     3360

aatcgctaca tgtatgctaa ctcgcgtcaa attgtgttta acacacgtcg taacaacaac     3420

gattttaacg aaggttataa aatcattatc aaacggatcc gcggcaatac gaacgatact     3480

cgtgttcgtg gcggtgacat tctgtatttc gacatgacga ttaataataa agcgtacaat     3540

ctgttcatga agaacgaaac catgtacgcc gataaccatt ccactgaaga tatctacgca     3600

atcggacttc gcgaacagac caaagacatt aacgacaaca tcatctttca gattcaaccg     3660

atgaataata cctactacta tgcctcccag atcttcaaaa gtaatttcaa cggcgaaaac     3720

atttcaggca tttgctcaat cggcacttat cggttccggt taggtggtga ttggtatcgt     3780

cacaactacc ttgttcccac agtgaaacaa ggcaactatg catcgctctt agaaagcaca     3840

tctacgcatt ggggttttgt gccagtcagt gaa                                  3873


<210>  12
<211>  1291
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide Sequence of rBoNT/C(0)

<400>  12

Met Pro Ile Thr Ile Asn Asn Phe Asn Tyr Ser Asp Pro Val Asp Asn 
1               5                   10                  15      


Lys Asn Ile Leu Tyr Leu Asp Thr His Leu Asn Thr Leu Ala Asn Glu 
            20                  25                  30          


Pro Glu Lys Ala Phe Arg Ile Thr Gly Asn Ile Trp Val Ile Pro Asp 
        35                  40                  45              


Arg Phe Ser Arg Asn Ser Asn Pro Asn Leu Asn Lys Pro Pro Arg Val 
    50                  55                  60                  


Thr Ser Pro Lys Ser Gly Tyr Tyr Asp Pro Asn Tyr Leu Ser Thr Asp 
65                  70                  75                  80  


Ser Asp Lys Asp Pro Phe Leu Lys Glu Ile Ile Lys Leu Phe Lys Arg 
                85                  90                  95      


Ile Asn Ser Arg Glu Ile Gly Glu Glu Leu Ile Tyr Arg Leu Ser Thr 
            100                 105                 110         


Asp Ile Pro Phe Pro Gly Asn Asn Asn Thr Pro Ile Asn Thr Phe Asp 
        115                 120                 125             


Phe Asp Val Asp Phe Asn Ser Val Asp Val Lys Thr Arg Gln Gly Asn 
    130                 135                 140                 


Asn Trp Val Lys Thr Gly Ser Ile Asn Pro Ser Val Ile Ile Thr Gly 
145                 150                 155                 160 


Pro Arg Glu Asn Ile Ile Asp Pro Glu Thr Ser Thr Phe Lys Leu Thr 
                165                 170                 175     


Asn Asn Thr Phe Ala Ala Gln Glu Gly Phe Gly Ala Leu Ser Ile Ile 
            180                 185                 190         


Ser Ile Ser Pro Arg Phe Met Leu Thr Tyr Ser Asn Ala Thr Asn Asp 
        195                 200                 205             


Val Gly Glu Gly Arg Phe Ser Lys Ser Glu Phe Cys Met Asp Pro Ile 
    210                 215                 220                 


Leu Ile Leu Met Gly Thr Leu Asn Asn Ala Met His Asn Leu Tyr Gly 
225                 230                 235                 240 


Ile Ala Ile Pro Asn Asp Gln Thr Ile Ser Ser Val Thr Ser Asn Ile 
                245                 250                 255     


Phe Tyr Ser Gln Tyr Asn Val Lys Leu Glu Tyr Ala Glu Ile Tyr Ala 
            260                 265                 270         


Phe Gly Gly Pro Thr Ile Asp Leu Ile Pro Lys Ser Ala Arg Lys Tyr 
        275                 280                 285             


Phe Glu Glu Lys Ala Leu Asp Tyr Tyr Arg Ser Ile Ala Lys Arg Leu 
    290                 295                 300                 


Asn Ser Ile Thr Thr Ala Asn Pro Ser Ser Phe Asn Lys Tyr Ile Gly 
305                 310                 315                 320 


Glu Tyr Lys Gln Lys Leu Ile Arg Lys Tyr Arg Phe Val Val Glu Ser 
                325                 330                 335     


Ser Gly Glu Val Thr Val Asn Arg Asn Lys Phe Val Glu Leu Tyr Asn 
            340                 345                 350         


Glu Leu Thr Gln Ile Phe Thr Glu Phe Asn Tyr Ala Lys Ile Tyr Asn 
        355                 360                 365             


Val Gln Asn Arg Lys Ile Tyr Leu Ser Asn Val Tyr Thr Pro Val Thr 
    370                 375                 380                 


Ala Asn Ile Leu Asp Asp Asn Val Tyr Asp Ile Gln Asn Gly Phe Asn 
385                 390                 395                 400 


Ile Pro Lys Ser Asn Leu Asn Val Leu Phe Met Gly Gln Asn Leu Ser 
                405                 410                 415     


Arg Asn Pro Ala Leu Arg Lys Val Asn Pro Glu Asn Met Leu Tyr Leu 
            420                 425                 430         


Phe Thr Lys Phe Cys His Lys Ala Ile Asp Gly Arg Ser Leu Tyr Asn 
        435                 440                 445             


Lys Thr Leu Asp Cys Arg Glu Leu Leu Val Lys Asn Thr Asp Leu Pro 
    450                 455                 460                 


Phe Ile Gly Asp Ile Ser Asp Val Lys Thr Asp Ile Phe Leu Arg Lys 
465                 470                 475                 480 


Asp Ile Asn Glu Glu Thr Glu Val Ile Tyr Tyr Pro Asp Asn Val Ser 
                485                 490                 495     


Val Asp Gln Val Ile Leu Ser Lys Asn Thr Ser Glu His Gly Gln Leu 
            500                 505                 510         


Asp Leu Leu Tyr Pro Ser Ile Asp Ser Glu Ser Glu Ile Leu Pro Gly 
        515                 520                 525             


Glu Asn Gln Val Phe Tyr Asp Asn Arg Thr Gln Asn Val Asp Tyr Leu 
    530                 535                 540                 


Asn Ser Tyr Tyr Tyr Leu Glu Ser Gln Lys Leu Ser Asp Asn Val Glu 
545                 550                 555                 560 


Asp Phe Thr Phe Thr Arg Ser Ile Glu Glu Ala Leu Asp Asn Ser Ala 
                565                 570                 575     


Lys Val Tyr Thr Tyr Phe Pro Thr Leu Ala Asn Lys Val Asn Ala Gly 
            580                 585                 590         


Val Gln Gly Gly Leu Phe Leu Met Trp Ala Asn Asp Val Val Glu Asp 
        595                 600                 605             


Phe Thr Thr Asn Ile Leu Arg Lys Asp Thr Leu Asp Lys Ile Ser Asp 
    610                 615                 620                 


Val Ser Ala Ile Ile Pro Tyr Ile Gly Pro Ala Leu Asn Ile Ser Asn 
625                 630                 635                 640 


Ser Val Arg Arg Gly Asn Phe Thr Glu Ala Phe Ala Val Thr Gly Val 
                645                 650                 655     


Thr Ile Leu Leu Glu Ala Phe Pro Glu Phe Thr Ile Pro Ala Leu Gly 
            660                 665                 670         


Ala Phe Val Ile Tyr Ser Lys Val Gln Glu Arg Asn Glu Ile Ile Lys 
        675                 680                 685             


Thr Ile Asp Asn Cys Leu Glu Gln Arg Ile Lys Arg Trp Lys Asp Ser 
    690                 695                 700                 


Tyr Glu Trp Met Met Gly Thr Trp Leu Ser Arg Ile Ile Thr Gln Phe 
705                 710                 715                 720 


Asn Asn Ile Ser Tyr Gln Met Tyr Asp Ser Leu Asn Tyr Gln Ala Gly 
                725                 730                 735     


Ala Ile Lys Ala Lys Ile Asp Leu Glu Tyr Lys Lys Tyr Ser Gly Ser 
            740                 745                 750         


Asp Lys Glu Asn Ile Lys Ser Gln Val Glu Asn Leu Lys Asn Ser Leu 
        755                 760                 765             


Asp Val Lys Ile Ser Glu Ala Met Asn Asn Ile Asn Lys Phe Ile Arg 
    770                 775                 780                 


Glu Cys Ser Val Thr Tyr Leu Phe Lys Asn Met Leu Pro Lys Val Ile 
785                 790                 795                 800 


Asp Glu Leu Asn Glu Phe Asp Arg Asn Thr Lys Ala Lys Leu Ile Asn 
                805                 810                 815     


Leu Ile Asp Ser His Asn Ile Ile Leu Val Gly Glu Val Asp Lys Leu 
            820                 825                 830         


Lys Ala Lys Val Asn Asn Ser Phe Gln Asn Thr Ile Pro Phe Asn Ile 
        835                 840                 845             


Phe Ser Tyr Thr Asn Asn Ser Leu Leu Lys Asp Ile Ile Asn Glu Tyr 
    850                 855                 860                 


Phe Asn Asn Ile Asn Asp Ser Lys Ile Leu Ser Leu Gln Asn Arg Lys 
865                 870                 875                 880 


Asn Thr Leu Val Asp Thr Ser Gly Tyr Asn Ala Glu Val Ser Glu Glu 
                885                 890                 895     


Gly Asp Val Gln Leu Asn Pro Ile Phe Pro Phe Asp Phe Lys Leu Gly 
            900                 905                 910         


Ser Ser Gly Glu Asp Arg Gly Lys Val Ile Val Thr Gln Asn Glu Asn 
        915                 920                 925             


Ile Val Tyr Asn Ser Met Tyr Glu Ser Phe Ser Ile Ser Phe Trp Ile 
    930                 935                 940                 


Arg Ile Asn Lys Trp Val Ser Asn Leu Pro Gly Tyr Thr Ile Ile Asp 
945                 950                 955                 960 


Ser Val Lys Asn Asn Ser Gly Trp Ser Ile Gly Ile Ile Ser Asn Phe 
                965                 970                 975     


Leu Val Phe Thr Leu Lys Gln Asn Glu Asp Ser Glu Gln Ser Ile Asn 
            980                 985                 990         


Phe Ser Tyr Asp Ile Ser Asn Asn  Ala Pro Gly Tyr Asn  Lys Trp Phe 
        995                 1000                 1005             


Phe Val  Thr Val Thr Asn Asn  Met Met Gly Asn Met  Lys Ile Tyr 
    1010                 1015                 1020             


Ile Asn  Gly Lys Leu Ile Asp  Thr Ile Lys Val Lys  Glu Leu Thr 
    1025                 1030                 1035             


Gly Ile  Asn Phe Ser Lys Thr  Ile Thr Phe Glu Ile  Asn Lys Ile 
    1040                 1045                 1050             


Pro Asp  Thr Gly Leu Ile Thr  Ser Asp Ser Asp Asn  Ile Asn Met 
    1055                 1060                 1065             


Trp Ile  Arg Asp Phe Tyr Ile  Phe Ala Lys Glu Leu  Asp Gly Lys 
    1070                 1075                 1080             


Asp Ile  Asn Ile Leu Phe Asn  Ser Leu Gln Tyr Thr  Asn Val Val 
    1085                 1090                 1095             


Lys Asp  Tyr Trp Gly Asn Asp  Leu Arg Tyr Asn Lys  Glu Tyr Tyr 
    1100                 1105                 1110             


Met Val  Asn Ile Asp Tyr Leu  Asn Arg Tyr Met Tyr  Ala Asn Ser 
    1115                 1120                 1125             


Arg Gln  Ile Val Phe Asn Thr  Arg Arg Asn Asn Asn  Asp Phe Asn 
    1130                 1135                 1140             


Glu Gly  Tyr Lys Ile Ile Ile  Lys Arg Ile Arg Gly  Asn Thr Asn 
    1145                 1150                 1155             


Asp Thr  Arg Val Arg Gly Gly  Asp Ile Leu Tyr Phe  Asp Met Thr 
    1160                 1165                 1170             


Ile Asn  Asn Lys Ala Tyr Asn  Leu Phe Met Lys Asn  Glu Thr Met 
    1175                 1180                 1185             


Tyr Ala  Asp Asn His Ser Thr  Glu Asp Ile Tyr Ala  Ile Gly Leu 
    1190                 1195                 1200             


Arg Glu  Gln Thr Lys Asp Ile  Asn Asp Asn Ile Ile  Phe Gln Ile 
    1205                 1210                 1215             


Gln Pro  Met Asn Asn Thr Tyr  Tyr Tyr Ala Ser Gln  Ile Phe Lys 
    1220                 1225                 1230             


Ser Asn  Phe Asn Gly Glu Asn  Ile Ser Gly Ile Cys  Ser Ile Gly 
    1235                 1240                 1245             


Thr Tyr  Arg Phe Arg Leu Gly  Gly Asp Trp Tyr Arg  His Asn Tyr 
    1250                 1255                 1260             


Leu Val  Pro Thr Val Lys Gln  Gly Asn Tyr Ala Ser  Leu Leu Glu 
    1265                 1270                 1275             


Ser Thr  Ser Thr His Trp Gly  Phe Val Pro Val Ser  Glu 
    1280                 1285                 1290     


<210>  13
<211>  3756
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide Sequence of rBoNT/E(0)

<400>  13
atgccgaaaa tcaactcttt caactacaac gacccggtta acgaccgtac catcctgtat       60

atcaaaccgg gtggttgcca ggagttctac aaatctttca acatcatgaa aaacatctgg      120

atcatcccgg aacgtaacgt tatcggtacc accccgcagg acttccaccc gccgacctct      180

ctgaaaaacg gtgactcttc ttactacgac ccgaactacc tccagtctga cgaagaaaaa      240

gaccgtttcc tgaaaatcgt taccaaaatc ttcaaccgta tcaacaacaa cctgtctggt      300

ggtatcctgc tggaagaact gtctaaagct aacccgtacc tgggtaacga caacaccccg      360

gacaaccagt tccacatcgg tgacgcttct gctgttgaaa tcaaattctc taacggttct      420

caggacatcc tgctgccgaa cgttatcatc atgggtgctg aaccggacct gttcgaaacc      480

aactcttcta acatctctct gcgtaacaac tacatgccgt ctaaccacgg tttcggttct      540

atcgctatcg ttaccttctc tccggaatac tctttccgtt tcaacgacaa cagcatgaac      600

gagttcatcc aggacccggc tctgaccctg atgcaccaac tgatctactc tctgcacggt      660

ctgtacggtg ctaaaggtat caccaccaaa tacaccatca cccagaaaca gaacccgctg      720

atcaccaaca tccgtggtac caacatcgaa gagttcctga ccttcggtgg taccgacctg      780

aacatcatca cctctgctca gtctaacgac atctacacca acctgctggc tgactacaaa      840

aaaatcgctt ctaaactgtc taaagttcag gtttctaacc cgctgctgaa cccgtacaaa      900

gacgttttcg aagctaaata cggtctggac aaagacgctt ctggtatcta ctctgttaac      960

atcaacaaat tcaacgacat cttcaaaaaa ctgtactctt tcaccgagtt cgacctggcg     1020

accaaattcc aggttaaatg ccgtcagacc tacatcggtc agtacaaata cttcaaactg     1080

tctaacctgc tgaacgactc tatctacaac atctctgaag gttacaacat caacaacctg     1140

aaagttaact tccgtggtca gaacgctaac ctgaacccgc gtatcatcac cccgatcacc     1200

ggtcgtggtc tggttaaaaa aatcatccgt ttctgcaaga atattgtaag cgttaaagga     1260

ataagaaaaa gtatctgcat cgaaatcaac aacggtgaac tgttcttcgt tgcttctgaa     1320

aactcttaca acgacgacaa catcaacacc ccgaaagaaa tcgacgacac cgttacctct     1380

aacaacaact acgaaaacga cctggaccag gttatcctga acttcaactc tgaatctgct     1440

ccgggtctgt ctgacgaaaa actgaacctg accatccaga acgacgctta catcccgaaa     1500

tacgactcta acggtacctc tgacatcgaa cagcacgacg ttaacgaact gaacgttttc     1560

ttctacctgg acgctcagaa agttccggaa ggtgaaaaca acgttaacct gacctcttct     1620

atcgacaccg ctctgctgga acagccgaaa atctacacct tcttctcttc tgagttcatc     1680

aacaacgtta acaaaccggt tcaggctgct ctgttcgttt cttggattca gcaggttctg     1740

gttgacttca ccaccgaagc taaccagaaa tctaccgttg acaaaatcgc tgacatctct     1800

atcgttgttc cgtacatcgg tctggctctg aacatcggta acgaagctca gaaaggtaac     1860

ttcaaagacg ctctggaact gctgggtgct ggtatcctgc tggagttcga accggaactg     1920

ctgatcccga ccatcctggt tttcaccatc aaatctttcc tgggttcttc tgacaacaaa     1980

aacaaagtta tcaaagctat caacaacgct ctgaaagaac gtgacgaaaa atggaaagaa     2040

gtttactctt tcatcgtttc taactggatg accaaaatca acacccagtt caacaaacgt     2100

aaagaacaga tgtaccaggc tctccagaac caggttaacg ctatcaaaac catcatcgaa     2160

tctaaataca actcttacac cctggaagaa aaaaacgaac tgaccaacaa atacgacatc     2220

aaacagatcg aaaacgaact gaaccagaaa gtttctatcg ctatgaacaa catcgaccgt     2280

ttcctgaccg aatcttctat ctcttacctg atgaaactca tcaacgaagt taaaatcaac     2340

aaactgcgtg aatacgacga aaacgttaaa acctacctgc tgaactacat catccagcac     2400

ggttctatcc tgggtgaatc tcagcaggaa ctgaactcta tggttaccga caccctgaac     2460

aactctatcc cgttcaaact gtcttcttac accgacgaca aaatcctgat ctcttacttc     2520

aacaaattct ttaaacgcat taagagttca tcggttctga atatgcggta caaaaatgat     2580

aaatatgtcg atacttctgg atatgatagc aatatcaaca ttaacggcga cgtgtataaa     2640

tatccgacaa ataaaaacca gtttgggata tataacgaca agctgtcgga ggtcaatatt     2700

tctcaaaacg actatatcat ttacgataat aaatataaaa actttagcat tagtttttgg     2760

gttcgtatac ctaattatga caataaaatt gtaaatgtga ataacgagta taccattata     2820

aactgtatgc gcgacaataa cagtggttgg aaggtatcgc tgaaccataa tgagattatc     2880

tggaccctgc aggataatgc aggtataaac cagaaactgg cttttaacta tggaaacgca     2940

aatgggatct cagattacat taataaatgg atttttgtta ccattacgaa cgatcgctta     3000

ggcgactcaa aactttatat taatggcaat ctgatagatc agaaatcaat cttaaatttg     3060

ggcaatattc atgtctctga taacatcttg ttcaagatcg ttaattgcag ttacactcgt     3120

tatattggca ttcgttactt taatatcttc gataaagaac tggacgagac ggaaatccag     3180

actctgtatt caaacgagcc caatactaat atattgaaag atttttgggg taactatctt     3240

ttatatgata aagaatacta tctcctgaat gtattgaagc caaacaattt catagataga     3300

cgcaaggata gcacattaag tatcaacaat atcagatcta ctatactgtt agcaaatcgc     3360

ctctactccg gtattaaagt gaagattcag cgggttaata actccagtac caatgataat     3420

ctggtccgta agaacgatca ggtatacatc aatttcgtcg cgagcaaaac tcatctcttc     3480

ccgctttacg ccgatacagc tacgacaaac aaggaaaaaa ccataaaaat ttccagctcc     3540

ggaaacagat tcaatcaagt agttgtaatg aactctgtgg gtaataattg tacgatgaac     3600

tttaagaata acaatgggaa caatattgga cttttgggct tcaaagccga cacagtggtg     3660

gcgtccacct ggtattacac gcacatgcgg gaccatacga attcgaacgg ttgcttctgg     3720

aactttatct cggaagaaca cgggtggcaa gaaaaa                               3756


<210>  14
<211>  1252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide Sequence of rBoNT/E(0)

<400>  14

Met Pro Lys Ile Asn Ser Phe Asn Tyr Asn Asp Pro Val Asn Asp Arg 
1               5                   10                  15      


Thr Ile Leu Tyr Ile Lys Pro Gly Gly Cys Gln Glu Phe Tyr Lys Ser 
            20                  25                  30          


Phe Asn Ile Met Lys Asn Ile Trp Ile Ile Pro Glu Arg Asn Val Ile 
        35                  40                  45              


Gly Thr Thr Pro Gln Asp Phe His Pro Pro Thr Ser Leu Lys Asn Gly 
    50                  55                  60                  


Asp Ser Ser Tyr Tyr Asp Pro Asn Tyr Leu Gln Ser Asp Glu Glu Lys 
65                  70                  75                  80  


Asp Arg Phe Leu Lys Ile Val Thr Lys Ile Phe Asn Arg Ile Asn Asn 
                85                  90                  95      


Asn Leu Ser Gly Gly Ile Leu Leu Glu Glu Leu Ser Lys Ala Asn Pro 
            100                 105                 110         


Tyr Leu Gly Asn Asp Asn Thr Pro Asp Asn Gln Phe His Ile Gly Asp 
        115                 120                 125             


Ala Ser Ala Val Glu Ile Lys Phe Ser Asn Gly Ser Gln Asp Ile Leu 
    130                 135                 140                 


Leu Pro Asn Val Ile Ile Met Gly Ala Glu Pro Asp Leu Phe Glu Thr 
145                 150                 155                 160 


Asn Ser Ser Asn Ile Ser Leu Arg Asn Asn Tyr Met Pro Ser Asn His 
                165                 170                 175     


Gly Phe Gly Ser Ile Ala Ile Val Thr Phe Ser Pro Glu Tyr Ser Phe 
            180                 185                 190         


Arg Phe Asn Asp Asn Ser Met Asn Glu Phe Ile Gln Asp Pro Ala Leu 
        195                 200                 205             


Thr Leu Met His Gln Leu Ile Tyr Ser Leu His Gly Leu Tyr Gly Ala 
    210                 215                 220                 


Lys Gly Ile Thr Thr Lys Tyr Thr Ile Thr Gln Lys Gln Asn Pro Leu 
225                 230                 235                 240 


Ile Thr Asn Ile Arg Gly Thr Asn Ile Glu Glu Phe Leu Thr Phe Gly 
                245                 250                 255     


Gly Thr Asp Leu Asn Ile Ile Thr Ser Ala Gln Ser Asn Asp Ile Tyr 
            260                 265                 270         


Thr Asn Leu Leu Ala Asp Tyr Lys Lys Ile Ala Ser Lys Leu Ser Lys 
        275                 280                 285             


Val Gln Val Ser Asn Pro Leu Leu Asn Pro Tyr Lys Asp Val Phe Glu 
    290                 295                 300                 


Ala Lys Tyr Gly Leu Asp Lys Asp Ala Ser Gly Ile Tyr Ser Val Asn 
305                 310                 315                 320 


Ile Asn Lys Phe Asn Asp Ile Phe Lys Lys Leu Tyr Ser Phe Thr Glu 
                325                 330                 335     


Phe Asp Leu Ala Thr Lys Phe Gln Val Lys Cys Arg Gln Thr Tyr Ile 
            340                 345                 350         


Gly Gln Tyr Lys Tyr Phe Lys Leu Ser Asn Leu Leu Asn Asp Ser Ile 
        355                 360                 365             


Tyr Asn Ile Ser Glu Gly Tyr Asn Ile Asn Asn Leu Lys Val Asn Phe 
    370                 375                 380                 


Arg Gly Gln Asn Ala Asn Leu Asn Pro Arg Ile Ile Thr Pro Ile Thr 
385                 390                 395                 400 


Gly Arg Gly Leu Val Lys Lys Ile Ile Arg Phe Cys Lys Asn Ile Val 
                405                 410                 415     


Ser Val Lys Gly Ile Arg Lys Ser Ile Cys Ile Glu Ile Asn Asn Gly 
            420                 425                 430         


Glu Leu Phe Phe Val Ala Ser Glu Asn Ser Tyr Asn Asp Asp Asn Ile 
        435                 440                 445             


Asn Thr Pro Lys Glu Ile Asp Asp Thr Val Thr Ser Asn Asn Asn Tyr 
    450                 455                 460                 


Glu Asn Asp Leu Asp Gln Val Ile Leu Asn Phe Asn Ser Glu Ser Ala 
465                 470                 475                 480 


Pro Gly Leu Ser Asp Glu Lys Leu Asn Leu Thr Ile Gln Asn Asp Ala 
                485                 490                 495     


Tyr Ile Pro Lys Tyr Asp Ser Asn Gly Thr Ser Asp Ile Glu Gln His 
            500                 505                 510         


Asp Val Asn Glu Leu Asn Val Phe Phe Tyr Leu Asp Ala Gln Lys Val 
        515                 520                 525             


Pro Glu Gly Glu Asn Asn Val Asn Leu Thr Ser Ser Ile Asp Thr Ala 
    530                 535                 540                 


Leu Leu Glu Gln Pro Lys Ile Tyr Thr Phe Phe Ser Ser Glu Phe Ile 
545                 550                 555                 560 


Asn Asn Val Asn Lys Pro Val Gln Ala Ala Leu Phe Val Ser Trp Ile 
                565                 570                 575     


Gln Gln Val Leu Val Asp Phe Thr Thr Glu Ala Asn Gln Lys Ser Thr 
            580                 585                 590         


Val Asp Lys Ile Ala Asp Ile Ser Ile Val Val Pro Tyr Ile Gly Leu 
        595                 600                 605             


Ala Leu Asn Ile Gly Asn Glu Ala Gln Lys Gly Asn Phe Lys Asp Ala 
    610                 615                 620                 


Leu Glu Leu Leu Gly Ala Gly Ile Leu Leu Glu Phe Glu Pro Glu Leu 
625                 630                 635                 640 


Leu Ile Pro Thr Ile Leu Val Phe Thr Ile Lys Ser Phe Leu Gly Ser 
                645                 650                 655     


Ser Asp Asn Lys Asn Lys Val Ile Lys Ala Ile Asn Asn Ala Leu Lys 
            660                 665                 670         


Glu Arg Asp Glu Lys Trp Lys Glu Val Tyr Ser Phe Ile Val Ser Asn 
        675                 680                 685             


Trp Met Thr Lys Ile Asn Thr Gln Phe Asn Lys Arg Lys Glu Gln Met 
    690                 695                 700                 


Tyr Gln Ala Leu Gln Asn Gln Val Asn Ala Ile Lys Thr Ile Ile Glu 
705                 710                 715                 720 


Ser Lys Tyr Asn Ser Tyr Thr Leu Glu Glu Lys Asn Glu Leu Thr Asn 
                725                 730                 735     


Lys Tyr Asp Ile Lys Gln Ile Glu Asn Glu Leu Asn Gln Lys Val Ser 
            740                 745                 750         


Ile Ala Met Asn Asn Ile Asp Arg Phe Leu Thr Glu Ser Ser Ile Ser 
        755                 760                 765             


Tyr Leu Met Lys Leu Ile Asn Glu Val Lys Ile Asn Lys Leu Arg Glu 
    770                 775                 780                 


Tyr Asp Glu Asn Val Lys Thr Tyr Leu Leu Asn Tyr Ile Ile Gln His 
785                 790                 795                 800 


Gly Ser Ile Leu Gly Glu Ser Gln Gln Glu Leu Asn Ser Met Val Thr 
                805                 810                 815     


Asp Thr Leu Asn Asn Ser Ile Pro Phe Lys Leu Ser Ser Tyr Thr Asp 
            820                 825                 830         


Asp Lys Ile Leu Ile Ser Tyr Phe Asn Lys Phe Phe Lys Arg Ile Lys 
        835                 840                 845             


Ser Ser Ser Val Leu Asn Met Arg Tyr Lys Asn Asp Lys Tyr Val Asp 
    850                 855                 860                 


Thr Ser Gly Tyr Asp Ser Asn Ile Asn Ile Asn Gly Asp Val Tyr Lys 
865                 870                 875                 880 


Tyr Pro Thr Asn Lys Asn Gln Phe Gly Ile Tyr Asn Asp Lys Leu Ser 
                885                 890                 895     


Glu Val Asn Ile Ser Gln Asn Asp Tyr Ile Ile Tyr Asp Asn Lys Tyr 
            900                 905                 910         


Lys Asn Phe Ser Ile Ser Phe Trp Val Arg Ile Pro Asn Tyr Asp Asn 
        915                 920                 925             


Lys Ile Val Asn Val Asn Asn Glu Tyr Thr Ile Ile Asn Cys Met Arg 
    930                 935                 940                 


Asp Asn Asn Ser Gly Trp Lys Val Ser Leu Asn His Asn Glu Ile Ile 
945                 950                 955                 960 


Trp Thr Leu Gln Asp Asn Ala Gly Ile Asn Gln Lys Leu Ala Phe Asn 
                965                 970                 975     


Tyr Gly Asn Ala Asn Gly Ile Ser Asp Tyr Ile Asn Lys Trp Ile Phe 
            980                 985                 990         


Val Thr Ile Thr Asn Asp Arg Leu  Gly Asp Ser Lys Leu  Tyr Ile Asn 
        995                 1000                 1005             


Gly Asn  Leu Ile Asp Gln Lys  Ser Ile Leu Asn Leu  Gly Asn Ile 
    1010                 1015                 1020             


His Val  Ser Asp Asn Ile Leu  Phe Lys Ile Val Asn  Cys Ser Tyr 
    1025                 1030                 1035             


Thr Arg  Tyr Ile Gly Ile Arg  Tyr Phe Asn Ile Phe  Asp Lys Glu 
    1040                 1045                 1050             


Leu Asp  Glu Thr Glu Ile Gln  Thr Leu Tyr Ser Asn  Glu Pro Asn 
    1055                 1060                 1065             


Thr Asn  Ile Leu Lys Asp Phe  Trp Gly Asn Tyr Leu  Leu Tyr Asp 
    1070                 1075                 1080             


Lys Glu  Tyr Tyr Leu Leu Asn  Val Leu Lys Pro Asn  Asn Phe Ile 
    1085                 1090                 1095             


Asp Arg  Arg Lys Asp Ser Thr  Leu Ser Ile Asn Asn  Ile Arg Ser 
    1100                 1105                 1110             


Thr Ile  Leu Leu Ala Asn Arg  Leu Tyr Ser Gly Ile  Lys Val Lys 
    1115                 1120                 1125             


Ile Gln  Arg Val Asn Asn Ser  Ser Thr Asn Asp Asn  Leu Val Arg 
    1130                 1135                 1140             


Lys Asn  Asp Gln Val Tyr Ile  Asn Phe Val Ala Ser  Lys Thr His 
    1145                 1150                 1155             


Leu Phe  Pro Leu Tyr Ala Asp  Thr Ala Thr Thr Asn  Lys Glu Lys 
    1160                 1165                 1170             


Thr Ile  Lys Ile Ser Ser Ser  Gly Asn Arg Phe Asn  Gln Val Val 
    1175                 1180                 1185             


Val Met  Asn Ser Val Gly Asn  Asn Cys Thr Met Asn  Phe Lys Asn 
    1190                 1195                 1200             


Asn Asn  Gly Asn Asn Ile Gly  Leu Leu Gly Phe Lys  Ala Asp Thr 
    1205                 1210                 1215             


Val Val  Ala Ser Thr Trp Tyr  Tyr Thr His Met Arg  Asp His Thr 
    1220                 1225                 1230             


Asn Ser  Asn Gly Cys Phe Trp  Asn Phe Ile Ser Glu  Glu His Gly 
    1235                 1240                 1245             


Trp Gln  Glu Lys 
    1250         


<210>  15
<211>  3834
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide Sequence of rBoNT/F(0)

<400>  15
atgccggtgg tcatcaacag cttcaactac aacgacccag taaacgacga cacgatcctg       60

tatatgcaaa tcccgtatga agagaagagc aagaagtact ataaggcctt tgaaatcatg      120

cgcaatgtgt ggattattcc ggagcgtaat acgattggta ctgacccaag cgacttcgat      180

ccacctgcgt ctttggaaaa cggctcgtcc gcatattacg acccgaatta cctgaccacc      240

gatgcggaga aagatcgtta tttgaaaacc accatcaagc tgttcaaacg cattaacagc      300

aatccggcag gtgaggtcct gctgcaagag attagctacg caaagcctta tctgggtaat      360

gagcatacgc ctattaacga gtttcacccg gttacccgca ctaccagcgt taacatcaag      420

tcctcgacca acgtgaagtc tagcattatc ctgaacctgc tggttctggg tgccggtccg      480

gacatcttcg aaaactctag ctacccggtg cgtaaactga tggatagcgg cggtgtttat      540

gacccgagca atgacggttt tggcagcatc aatatcgtga cgtttagccc ggagtacgag      600

tacaccttca atgatatcag cggtggttac aattcttcta ccgagagctt catcgccgac      660

ccggcgatca gcctggcaca ccaactgatc tatgcattgc atggcttgta cggtgcccgt      720

ggtgtgacgt ataaagagac tatcaaggtt aagcaggcac ctctgatgat tgcggaaaag      780

ccgattcgcc tggaagagtt cctgaccttc ggcggtcaag atttgaacat cattacctcg      840

gccatgaaag agaaaatcta taacaatttg ctggccaact atgaaaagat tgcaacgcgc      900

ttgtctcgtg ttaactccgc tccgccggaa tacgacatta atgagtacaa agactacttt      960

caatggaaat atggcctgga caaaaatgcg gatggttctt ataccgtgaa tgaaaacaaa     1020

ttcaatgaaa tctacaagaa actgtacagc ttcaccgaaa tcgatctggc gaacaagttc     1080

aaagtcaaat gtcgtaatac ctacttcatc aaatatggct tcctgaaagt cccgaacctg     1140

ctggacgatg acatctatac cgtcagcgaa ggcttcaaca tcggcaatct ggccgtgaat     1200

aatcgtggtc agaacatcaa actgaatccg aaaatcattg actccatccc agacaagggc     1260

ctggttgaga aaatcgtgaa gttctgcaaa agcgttattc cgcgtaaagg tacgaaagca     1320

ccgcctcgcc tgtgcattcg cgttaacaac cgtgagttgt tctttgtggc atctgaaagc     1380

agctacaacg agaacgacat caacacccct aaagaaattg atgataccac gaacctgaat     1440

aacaattatc gcaacaatct ggacgaggtg atcctggatt acaattcgga aaccattccg     1500

caaattagca atcagacgct gaacaccctg gttcaggacg atagctacgt tccgcgttac     1560

gactccaatg gtactagcga gattgaagaa cacaacgtag tggacttgaa cgttttcttt     1620

tatctgcacg cccagaaggt tccggagggc gaaaccaata ttagcctgac cagctcgatc     1680

gacaccgcgc tgtctgagga gagccaagtc tacacctttt tcagcagcga gtttatcaac     1740

actattaaca agccagttca tgctgcattg tttatctctt ggattaacca ggtgattcgc     1800

gactttacga cggaggcgac ccagaagtct accttcgaca aaattgcaga catctccctg     1860

gtcgtcccat acgtcggcct ggcgttgaat attggcaatg aagttcaaaa agagaacttc     1920

aaagaagcgt tcgagctgct gggtgcaggc atcctgctgg agttcgtgcc ggaactgttg     1980

atcccgacca tcctggtgtt caccattaag agcttcattg gatcctccga gaataagaac     2040

aagatcatca aggcgatcaa taacagcctg atggagcgtg aaacgaagtg gaaagaaatc     2100

tatagctgga ttgttagcaa ttggctgact cgtattaaca cgcaattcaa caagcgtaaa     2160

gagcaaatgt accaagccct gcaaaaccaa gttgacgcca tcaaaacggt aattgaatac     2220

aagtacaaca attacacgag cgatgagcgc aaccgcctgg aaagcgaata caacatcaac     2280

aacattcgcg aagaattgaa caagaaagtg agcctggcga tggagaacat tgagcgtttt     2340

atcaccgaaa gcagcatctt ttacctgatg aaattgatta atgaggcgaa agtctcgaaa     2400

ctgcgtgagt acgacgaagg tgtgaaagag tatctgctgg attacattag cgagcaccgt     2460

agcatcttgg gtaactcggt tcaggagctg aacgatctgg tgacctctac cctgaacaat     2520

agcatcccgt tcgaactgag cagctatacc aatgacaaga ttctgattct gtatttcaat     2580

aaactgtata agaagatcaa ggataacagc attctggata tgcgttacga aaacaataag     2640

tttatcgaca tttctggtta cggcagcaac atttccatca atggcgatgt ctacatctac     2700

agcaccaatc gcaaccagtt cggcatctac tctagcaaac cgagcgaagt taacatcgca     2760

cagaacaatg atattattta taacggtcgt tatcaaaact tctctatcag cttttgggtc     2820

cgtatcccga agtacttcaa taaagtcaat ctgaataatg aatacacgat catcgactgc     2880

attcgcaata acaacagcgg ttggaaaatc agcctgaatt acaacaaaat tatttggacc     2940

ctgcaagata cggcgggtaa caatcagaaa ctggtgttta actacacgca aatgatcagc     3000

atttctgact atatcaacaa gtggatcttt gttaccatca ccaataatcg tctgggcaat     3060

agccgtattt acatcaacgg taacctgatt gatgagaaaa gcatcagcaa cctgggcgat     3120

attcacgtca gcgacaacat tctgttcaaa attgttggtt gtaacgatac ccgttacgtc     3180

ggcatccgtt atttcaaggt tttcgatacg gagctgggta aaacggaaat cgaaacgttg     3240

tactccgatg aaccagatcc gagcattctg aaggactttt ggggtaacta cttgctgtac     3300

aataaacgtt actatctgct gaatctgttg cgcaccgaca agagcattac ccaaaacagc     3360

aatttcctga acattaatca gcaacgcggc gtataccaaa aaccgaacat cttcagcaat     3420

acgcgcctgt atactggtgt tgaagtgatc attcgtaaga acggtagcac cgacattagc     3480

aacacggaca atttcgtccg taagaatgac ctggcgtaca ttaacgtcgt ggaccgtgat     3540

gtcgagtatc gtctgtacgc agacatcagc attgcgaaac cggaaaagat tatcaagctg     3600

atccgtacca gcaacagcaa caacagcctg ggtcagatca ttgtgatgga cagcattggt     3660

aataactgca cgatgaactt ccagaacaac aatggtggta atatcggtct gctgggtttt     3720

cacagcaata atctggttgc ttccagctgg tactacaata acattcgtaa aaacacgtct     3780

agcaatggtt gtttttggag ctttatcagc aaagagcacg gctggcaaga aaat           3834


<210>  16
<211>  1278
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide Sequence of rBoNT/F(0)

<400>  16

Met Pro Val Val Ile Asn Ser Phe Asn Tyr Asn Asp Pro Val Asn Asp 
1               5                   10                  15      


Asp Thr Ile Leu Tyr Met Gln Ile Pro Tyr Glu Glu Lys Ser Lys Lys 
            20                  25                  30          


Tyr Tyr Lys Ala Phe Glu Ile Met Arg Asn Val Trp Ile Ile Pro Glu 
        35                  40                  45              


Arg Asn Thr Ile Gly Thr Asp Pro Ser Asp Phe Asp Pro Pro Ala Ser 
    50                  55                  60                  


Leu Glu Asn Gly Ser Ser Ala Tyr Tyr Asp Pro Asn Tyr Leu Thr Thr 
65                  70                  75                  80  


Asp Ala Glu Lys Asp Arg Tyr Leu Lys Thr Thr Ile Lys Leu Phe Lys 
                85                  90                  95      


Arg Ile Asn Ser Asn Pro Ala Gly Glu Val Leu Leu Gln Glu Ile Ser 
            100                 105                 110         


Tyr Ala Lys Pro Tyr Leu Gly Asn Glu His Thr Pro Ile Asn Glu Phe 
        115                 120                 125             


His Pro Val Thr Arg Thr Thr Ser Val Asn Ile Lys Ser Ser Thr Asn 
    130                 135                 140                 


Val Lys Ser Ser Ile Ile Leu Asn Leu Leu Val Leu Gly Ala Gly Pro 
145                 150                 155                 160 


Asp Ile Phe Glu Asn Ser Ser Tyr Pro Val Arg Lys Leu Met Asp Ser 
                165                 170                 175     


Gly Gly Val Tyr Asp Pro Ser Asn Asp Gly Phe Gly Ser Ile Asn Ile 
            180                 185                 190         


Val Thr Phe Ser Pro Glu Tyr Glu Tyr Thr Phe Asn Asp Ile Ser Gly 
        195                 200                 205             


Gly Tyr Asn Ser Ser Thr Glu Ser Phe Ile Ala Asp Pro Ala Ile Ser 
    210                 215                 220                 


Leu Ala His Gln Leu Ile Tyr Ala Leu His Gly Leu Tyr Gly Ala Arg 
225                 230                 235                 240 


Gly Val Thr Tyr Lys Glu Thr Ile Lys Val Lys Gln Ala Pro Leu Met 
                245                 250                 255     


Ile Ala Glu Lys Pro Ile Arg Leu Glu Glu Phe Leu Thr Phe Gly Gly 
            260                 265                 270         


Gln Asp Leu Asn Ile Ile Thr Ser Ala Met Lys Glu Lys Ile Tyr Asn 
        275                 280                 285             


Asn Leu Leu Ala Asn Tyr Glu Lys Ile Ala Thr Arg Leu Ser Arg Val 
    290                 295                 300                 


Asn Ser Ala Pro Pro Glu Tyr Asp Ile Asn Glu Tyr Lys Asp Tyr Phe 
305                 310                 315                 320 


Gln Trp Lys Tyr Gly Leu Asp Lys Asn Ala Asp Gly Ser Tyr Thr Val 
                325                 330                 335     


Asn Glu Asn Lys Phe Asn Glu Ile Tyr Lys Lys Leu Tyr Ser Phe Thr 
            340                 345                 350         


Glu Ile Asp Leu Ala Asn Lys Phe Lys Val Lys Cys Arg Asn Thr Tyr 
        355                 360                 365             


Phe Ile Lys Tyr Gly Phe Leu Lys Val Pro Asn Leu Leu Asp Asp Asp 
    370                 375                 380                 


Ile Tyr Thr Val Ser Glu Gly Phe Asn Ile Gly Asn Leu Ala Val Asn 
385                 390                 395                 400 


Asn Arg Gly Gln Asn Ile Lys Leu Asn Pro Lys Ile Ile Asp Ser Ile 
                405                 410                 415     


Pro Asp Lys Gly Leu Val Glu Lys Ile Val Lys Phe Cys Lys Ser Val 
            420                 425                 430         


Ile Pro Arg Lys Gly Thr Lys Ala Pro Pro Arg Leu Cys Ile Arg Val 
        435                 440                 445             


Asn Asn Arg Glu Leu Phe Phe Val Ala Ser Glu Ser Ser Tyr Asn Glu 
    450                 455                 460                 


Asn Asp Ile Asn Thr Pro Lys Glu Ile Asp Asp Thr Thr Asn Leu Asn 
465                 470                 475                 480 


Asn Asn Tyr Arg Asn Asn Leu Asp Glu Val Ile Leu Asp Tyr Asn Ser 
                485                 490                 495     


Glu Thr Ile Pro Gln Ile Ser Asn Gln Thr Leu Asn Thr Leu Val Gln 
            500                 505                 510         


Asp Asp Ser Tyr Val Pro Arg Tyr Asp Ser Asn Gly Thr Ser Glu Ile 
        515                 520                 525             


Glu Glu His Asn Val Val Asp Leu Asn Val Phe Phe Tyr Leu His Ala 
    530                 535                 540                 


Gln Lys Val Pro Glu Gly Glu Thr Asn Ile Ser Leu Thr Ser Ser Ile 
545                 550                 555                 560 


Asp Thr Ala Leu Ser Glu Glu Ser Gln Val Tyr Thr Phe Phe Ser Ser 
                565                 570                 575     


Glu Phe Ile Asn Thr Ile Asn Lys Pro Val His Ala Ala Leu Phe Ile 
            580                 585                 590         


Ser Trp Ile Asn Gln Val Ile Arg Asp Phe Thr Thr Glu Ala Thr Gln 
        595                 600                 605             


Lys Ser Thr Phe Asp Lys Ile Ala Asp Ile Ser Leu Val Val Pro Tyr 
    610                 615                 620                 


Val Gly Leu Ala Leu Asn Ile Gly Asn Glu Val Gln Lys Glu Asn Phe 
625                 630                 635                 640 


Lys Glu Ala Phe Glu Leu Leu Gly Ala Gly Ile Leu Leu Glu Phe Val 
                645                 650                 655     


Pro Glu Leu Leu Ile Pro Thr Ile Leu Val Phe Thr Ile Lys Ser Phe 
            660                 665                 670         


Ile Gly Ser Ser Glu Asn Lys Asn Lys Ile Ile Lys Ala Ile Asn Asn 
        675                 680                 685             


Ser Leu Met Glu Arg Glu Thr Lys Trp Lys Glu Ile Tyr Ser Trp Ile 
    690                 695                 700                 


Val Ser Asn Trp Leu Thr Arg Ile Asn Thr Gln Phe Asn Lys Arg Lys 
705                 710                 715                 720 


Glu Gln Met Tyr Gln Ala Leu Gln Asn Gln Val Asp Ala Ile Lys Thr 
                725                 730                 735     


Val Ile Glu Tyr Lys Tyr Asn Asn Tyr Thr Ser Asp Glu Arg Asn Arg 
            740                 745                 750         


Leu Glu Ser Glu Tyr Asn Ile Asn Asn Ile Arg Glu Glu Leu Asn Lys 
        755                 760                 765             


Lys Val Ser Leu Ala Met Glu Asn Ile Glu Arg Phe Ile Thr Glu Ser 
    770                 775                 780                 


Ser Ile Phe Tyr Leu Met Lys Leu Ile Asn Glu Ala Lys Val Ser Lys 
785                 790                 795                 800 


Leu Arg Glu Tyr Asp Glu Gly Val Lys Glu Tyr Leu Leu Asp Tyr Ile 
                805                 810                 815     


Ser Glu His Arg Ser Ile Leu Gly Asn Ser Val Gln Glu Leu Asn Asp 
            820                 825                 830         


Leu Val Thr Ser Thr Leu Asn Asn Ser Ile Pro Phe Glu Leu Ser Ser 
        835                 840                 845             


Tyr Thr Asn Asp Lys Ile Leu Ile Leu Tyr Phe Asn Lys Leu Tyr Lys 
    850                 855                 860                 


Lys Ile Lys Asp Asn Ser Ile Leu Asp Met Arg Tyr Glu Asn Asn Lys 
865                 870                 875                 880 


Phe Ile Asp Ile Ser Gly Tyr Gly Ser Asn Ile Ser Ile Asn Gly Asp 
                885                 890                 895     


Val Tyr Ile Tyr Ser Thr Asn Arg Asn Gln Phe Gly Ile Tyr Ser Ser 
            900                 905                 910         


Lys Pro Ser Glu Val Asn Ile Ala Gln Asn Asn Asp Ile Ile Tyr Asn 
        915                 920                 925             


Gly Arg Tyr Gln Asn Phe Ser Ile Ser Phe Trp Val Arg Ile Pro Lys 
    930                 935                 940                 


Tyr Phe Asn Lys Val Asn Leu Asn Asn Glu Tyr Thr Ile Ile Asp Cys 
945                 950                 955                 960 


Ile Arg Asn Asn Asn Ser Gly Trp Lys Ile Ser Leu Asn Tyr Asn Lys 
                965                 970                 975     


Ile Ile Trp Thr Leu Gln Asp Thr Ala Gly Asn Asn Gln Lys Leu Val 
            980                 985                 990         


Phe Asn Tyr Thr Gln Met Ile Ser  Ile Ser Asp Tyr Ile  Asn Lys Trp 
        995                 1000                 1005             


Ile Phe  Val Thr Ile Thr Asn  Asn Arg Leu Gly Asn  Ser Arg Ile 
    1010                 1015                 1020             


Tyr Ile  Asn Gly Asn Leu Ile  Asp Glu Lys Ser Ile  Ser Asn Leu 
    1025                 1030                 1035             


Gly Asp  Ile His Val Ser Asp  Asn Ile Leu Phe Lys  Ile Val Gly 
    1040                 1045                 1050             


Cys Asn  Asp Thr Arg Tyr Val  Gly Ile Arg Tyr Phe  Lys Val Phe 
    1055                 1060                 1065             


Asp Thr  Glu Leu Gly Lys Thr  Glu Ile Glu Thr Leu  Tyr Ser Asp 
    1070                 1075                 1080             


Glu Pro  Asp Pro Ser Ile Leu  Lys Asp Phe Trp Gly  Asn Tyr Leu 
    1085                 1090                 1095             


Leu Tyr  Asn Lys Arg Tyr Tyr  Leu Leu Asn Leu Leu  Arg Thr Asp 
    1100                 1105                 1110             


Lys Ser  Ile Thr Gln Asn Ser  Asn Phe Leu Asn Ile  Asn Gln Gln 
    1115                 1120                 1125             


Arg Gly  Val Tyr Gln Lys Pro  Asn Ile Phe Ser Asn  Thr Arg Leu 
    1130                 1135                 1140             


Tyr Thr  Gly Val Glu Val Ile  Ile Arg Lys Asn Gly  Ser Thr Asp 
    1145                 1150                 1155             


Ile Ser  Asn Thr Asp Asn Phe  Val Arg Lys Asn Asp  Leu Ala Tyr 
    1160                 1165                 1170             


Ile Asn  Val Val Asp Arg Asp  Val Glu Tyr Arg Leu  Tyr Ala Asp 
    1175                 1180                 1185             


Ile Ser  Ile Ala Lys Pro Glu  Lys Ile Ile Lys Leu  Ile Arg Thr 
    1190                 1195                 1200             


Ser Asn  Ser Asn Asn Ser Leu  Gly Gln Ile Ile Val  Met Asp Ser 
    1205                 1210                 1215             


Ile Gly  Asn Asn Cys Thr Met  Asn Phe Gln Asn Asn  Asn Gly Gly 
    1220                 1225                 1230             


Asn Ile  Gly Leu Leu Gly Phe  His Ser Asn Asn Leu  Val Ala Ser 
    1235                 1240                 1245             


Ser Trp  Tyr Tyr Asn Asn Ile  Arg Lys Asn Thr Ser  Ser Asn Gly 
    1250                 1255                 1260             


Cys Phe  Trp Ser Phe Ile Ser  Lys Glu His Gly Trp  Gln Glu Asn 
    1265                 1270                 1275             


<210>  17
<211>  3942
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide Sequence of rBoNT/A(0) (His-tagged)

<400>  17
atgccgtttg tgaacaagca gttcaactat aaagatccgg ttaatggtgt ggatatcgcc       60

tatatcaaaa ttccgaatgc aggtcagatg cagccggtta aagcctttaa aatccataac      120

aaaatttggg tgattccgga acgtgatacc tttaccaatc cggaagaagg tgatctgaat      180

ccgcctccgg aagcaaaaca ggttccggtt agctattatg atagcaccta tctgagcacc      240

gataacgaga aagataacta tctgaaaggt gtgaccaaac tgtttgaacg catttatagt      300

accgatctgg gtcgtatgct gctgaccagc attgttcgtg gtattccgtt ttggggtggt      360

agcaccattg ataccgaact gaaagttatt gacaccaact gcattaatgt gattcagccg      420

gatggtagct atcgtagcga agaactgaat ctggttatta ttggtccgag cgcagatatc      480

attcagtttg aatgtaaaag ctttggccac gaagttctga atctgacccg taatggttat      540

ggtagtaccc agtatattcg tttcagtccg gattttacct ttggctttga agaaagcctg      600

gaagttgata caaatccgct gttaggtgca ggtaaatttg caaccgatcc ggcagttacc      660

ctggcacacc agctgattta tgccggtcat cgtctgtatg gtattgccat taatccgaat      720

cgtgtgttca aagtgaatac caacgcctat tatgaaatga gcggtctgga agtgagtttt      780

gaagaactgc gtacctttgg tggtcatgat gccaaattta tcgatagcct gcaagaaaat      840

gaatttcgcc tgtactacta taacaaattc aaggatattg cgagcaccct gaataaagcc      900

aaaagcattg ttggcaccac cgcaagcctg cagtatatga aaaatgtgtt taaagaaaaa      960

tatctgctga gcgaagatac cagcggtaaa tttagcgttg acaaactgaa attcgataaa     1020

ctgtacaaga tgctgaccga gatttatacc gaagataact tcgtgaagtt tttcaaagtg     1080

ctgaaccgca aaacctacct gaactttgat aaagccgtgt tcaaaatcaa catcgtgccg     1140

aaagtgaact ataccatcta tgatggtttt aacctgcgca ataccaatct ggcagcaaac     1200

tttaatggtc agaacaccga aatcaacaac atgaacttta ccaaactgaa gaacttcacc     1260

ggtctgttcg aattttacaa actgctgtgt gttcgtggca ttattaccag caaaaccaaa     1320

agtctggata aaggctacaa taaagccctg aatgatctgt gcattaaggt gaataattgg     1380

gacctgtttt ttagcccgag cgaggataat ttcaccaacg atctgaacaa aggcgaagaa     1440

attaccagcg ataccaatat tgaagcagcc gaagaaaaca ttagcctgga tctgattcag     1500

cagtattatc tgaccttcaa cttcgataat gagccggaaa atatcagcat tgaaaacctg     1560

agcagcgata ttattggcca gctggaactg atgccgaata ttgaacgttt tccgaacggc     1620

aaaaaatacg agctggataa atacaccatg ttccattatc tgcgtgccca agaatttgaa     1680

catggtaaaa gccgtattgc actgaccaat agcgttaatg aagcactgct gaacccgagc     1740

cgtgtttata ccttttttag cagcgattac gtgaaaaagg ttaacaaagc aaccgaagca     1800

gccatgtttt taggttgggt tgaacagctg gtttatgatt tcaccgatga aaccagcgaa     1860

gttagcacca ccgataaaat tgcagatatt accatcatca tcccgtatat cggtccggca     1920

ctgaatattg gcaatatgct gtataaagac gattttgtgg gtgccctgat ttttagcggt     1980

gcagttattc tgctggaatt tattccggaa attgccattc cggttctggg cacctttgca     2040

ctggtgagct atattgcaaa taaagttctg accgtgcaga ccatcgataa tgcactgagc     2100

aaacgtaacg aaaaatggga tgaagtgtac aagtatatcg tgaccaattg gctggcaaaa     2160

gttaacaccc agattgacct gattcgcaag aagatgaaag aagcactgga aaatcaggca     2220

gaagcaacca aagccattat caactatcag tataaccagt acaccgaaga agagaaaaat     2280

aacatcaact tcaacatcga cgatctgtcc agcaaactga acgaaagcat caacaaagcc     2340

atgattaaca ttaacaaatt tctgaaccag tgcagcgtga gctatctgat gaatagcatg     2400

attccgtatg gtgtgaaacg tctggaagat tttgatgcaa gcctgaaaga tgccctgctg     2460

aaatatatct atgataatcg tggcaccctg attggtcagg ttgatcgtct gaaagataaa     2520

gtgaacaaca ccctgagtac cgatattcct tttcagctga gcaaatatgt ggataatcag     2580

cgtctgctgt caacctttac cgaatacatt aagaacatca tcaacaccag cattctgaac     2640

ctgcgttatg aaagcaatca tctgattgat ctgagccgtt atgccagcaa aatcaatata     2700

ggcagcaagg ttaacttcga cccgattgac aaaaatcaga tacagctgtt taatctggaa     2760

agcagcaaaa ttgaggtgat cctgaaaaac gccattgtgt ataatagcat gtacgagaat     2820

ttctcgacca gcttttggat tcgtatcccg aaatacttta atagcatcag cctgaacaac     2880

gagtacacca ttattaactg catggaaaac aatagcggct ggaaagttag cctgaattat     2940

ggcgaaatta tctggaccct gcaggatacc caagaaatca aacagcgtgt ggttttcaaa     3000

tacagccaga tgattaatat cagcgactat atcaaccgct ggatttttgt gaccattacc     3060

aataatcgcc tgaataacag caagatctat attaacggtc gtctgattga ccagaaaccg     3120

attagtaatc tgggtaatat tcatgcgagc aacaacatca tgtttaaact ggatggttgt     3180

cgtgataccc atcgttatat ttggatcaag tacttcaacc tgttcgataa agagttgaac     3240

gaaaaagaaa ttaaagacct gtatgataac cagagcaaca gcggtattct gaaggatttt     3300

tggggagatt atctgcagta tgacaaaccg tattatatgc tgaatctgta cgacccgaat     3360

aaatacgtgg atgtgaataa tgttggcatc cgtggttata tgtacctgaa aggtccgcgt     3420

ggtagcgtta tgaccacaaa catttatctg aatagcagcc tgtatcgcgg aaccaaattc     3480

atcattaaaa agtatgccag cggcaacaag gataatattg tgcgtaataa tgatcgcgtg     3540

tacattaacg ttgtggtgaa gaataaagaa tatcgcctgg caaccaatgc aagccaggca     3600

ggcgttgaaa aaattctgag tgccctggaa attccggatg ttggtaatct gagccaggtt     3660

gttgtgatga aaagcaaaaa tgatcagggc atcaccaaca agtgcaaaat gaatctgcag     3720

gacaataacg gcaacgatat tggttttatt ggcttccacc agttcaacaa tattgcgaaa     3780

ctggttgcaa gcaattggta taatcgtcag attgaacgta gcagtcgtac cctgggttgt     3840

agctgggaat ttatccctgt ggatgatggt tggggtgaac gtccgctgga aaacctgtat     3900

tttcaaggtg caagtcatca tcaccatcac caccatcatt aa                        3942


<210>  18
<211>  1313
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide Sequence of rBoNT/A(0) (His-tagged)

<400>  18

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Gln 
    210                 215                 220                 


Leu Ile Tyr Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly Tyr Asn Lys 
        435                 440                 445             


Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe 
    450                 455                 460                 


Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu 
465                 470                 475                 480 


Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu 
                485                 490                 495     


Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro 
            500                 505                 510         


Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu 
        515                 520                 525             


Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu 
    530                 535                 540                 


Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu 
545                 550                 555                 560 


His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu 
                565                 570                 575     


Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys 
            580                 585                 590         


Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu 
        595                 600                 605             


Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr 
    610                 615                 620                 


Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala 
625                 630                 635                 640 


Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu 
                645                 650                 655     


Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala 
            660                 665                 670         


Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys 
        675                 680                 685             


Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu 
    690                 695                 700                 


Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys 
705                 710                 715                 720 


Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu 
                725                 730                 735     


Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn 
            740                 745                 750         


Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp 
        755                 760                 765             


Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile 
    770                 775                 780                 


Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met 
785                 790                 795                 800 


Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys 
                805                 810                 815     


Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly 
            820                 825                 830         


Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp 
        835                 840                 845             


Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser 
    850                 855                 860                 


Thr Phe Thr Glu Tyr Ile Lys Asn Ile Ile Asn Thr Ser Ile Leu Asn 
865                 870                 875                 880 


Leu Arg Tyr Glu Ser Asn His Leu Ile Asp Leu Ser Arg Tyr Ala Ser 
                885                 890                 895     


Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp Pro Ile Asp Lys Asn 
            900                 905                 910         


Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys Ile Glu Val Ile Leu 
        915                 920                 925             


Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr Glu Asn Phe Ser Thr Ser 
    930                 935                 940                 


Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn Ser Ile Ser Leu Asn Asn 
945                 950                 955                 960 


Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn Ser Gly Trp Lys Val 
                965                 970                 975     


Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr Leu Gln Asp Thr Gln Glu 
            980                 985                 990         


Ile Lys Gln Arg Val Val Phe Lys  Tyr Ser Gln Met Ile  Asn Ile Ser 
        995                 1000                 1005             


Asp Tyr  Ile Asn Arg Trp Ile  Phe Val Thr Ile Thr  Asn Asn Arg 
    1010                 1015                 1020             


Leu Asn  Asn Ser Lys Ile Tyr  Ile Asn Gly Arg Leu  Ile Asp Gln 
    1025                 1030                 1035             


Lys Pro  Ile Ser Asn Leu Gly  Asn Ile His Ala Ser  Asn Asn Ile 
    1040                 1045                 1050             


Met Phe  Lys Leu Asp Gly Cys  Arg Asp Thr His Arg  Tyr Ile Trp 
    1055                 1060                 1065             


Ile Lys  Tyr Phe Asn Leu Phe  Asp Lys Glu Leu Asn  Glu Lys Glu 
    1070                 1075                 1080             


Ile Lys  Asp Leu Tyr Asp Asn  Gln Ser Asn Ser Gly  Ile Leu Lys 
    1085                 1090                 1095             


Asp Phe  Trp Gly Asp Tyr Leu  Gln Tyr Asp Lys Pro  Tyr Tyr Met 
    1100                 1105                 1110             


Leu Asn  Leu Tyr Asp Pro Asn  Lys Tyr Val Asp Val  Asn Asn Val 
    1115                 1120                 1125             


Gly Ile  Arg Gly Tyr Met Tyr  Leu Lys Gly Pro Arg  Gly Ser Val 
    1130                 1135                 1140             


Met Thr  Thr Asn Ile Tyr Leu  Asn Ser Ser Leu Tyr  Arg Gly Thr 
    1145                 1150                 1155             


Lys Phe  Ile Ile Lys Lys Tyr  Ala Ser Gly Asn Lys  Asp Asn Ile 
    1160                 1165                 1170             


Val Arg  Asn Asn Asp Arg Val  Tyr Ile Asn Val Val  Val Lys Asn 
    1175                 1180                 1185             


Lys Glu  Tyr Arg Leu Ala Thr  Asn Ala Ser Gln Ala  Gly Val Glu 
    1190                 1195                 1200             


Lys Ile  Leu Ser Ala Leu Glu  Ile Pro Asp Val Gly  Asn Leu Ser 
    1205                 1210                 1215             


Gln Val  Val Val Met Lys Ser  Lys Asn Asp Gln Gly  Ile Thr Asn 
    1220                 1225                 1230             


Lys Cys  Lys Met Asn Leu Gln  Asp Asn Asn Gly Asn  Asp Ile Gly 
    1235                 1240                 1245             


Phe Ile  Gly Phe His Gln Phe  Asn Asn Ile Ala Lys  Leu Val Ala 
    1250                 1255                 1260             


Ser Asn  Trp Tyr Asn Arg Gln  Ile Glu Arg Ser Ser  Arg Thr Leu 
    1265                 1270                 1275             


Gly Cys  Ser Trp Glu Phe Ile  Pro Val Asp Asp Gly  Trp Gly Glu 
    1280                 1285                 1290             


Arg Pro  Leu Glu Asn Leu Tyr  Phe Gln Gly Ala Ser  His His His 
    1295                 1300                 1305             


His His  His His His 
    1310             


<210>  19
<211>  2649
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide Sequence of rLHN/A (His-tagged)

<400>  19
atgccgtttg tgaacaagca gttcaactat aaagatccgg ttaatggtgt ggatatcgcc       60

tatatcaaaa ttccgaatgc aggtcagatg cagccggtta aagcctttaa aatccataac      120

aaaatttggg tgattccgga acgtgatacc tttaccaatc cggaagaagg tgatctgaat      180

ccgcctccgg aagcaaaaca ggttccggtt agctattatg atagcaccta tctgagcacc      240

gataacgaga aagataacta tctgaaaggt gtgaccaaac tgtttgaacg catttatagt      300

accgatctgg gtcgtatgct gctgaccagc attgttcgtg gtattccgtt ttggggtggt      360

agcaccattg ataccgaact gaaagttatt gacaccaact gcattaatgt gattcagccg      420

gatggtagct atcgtagcga agaactgaat ctggttatta ttggtccgag cgcagatatc      480

attcagtttg aatgtaaatc ctttggccac gaagttctga atctgacccg taatggttat      540

ggtagtaccc agtatattcg tttcagtccg gattttacct ttggctttga agaaagcctg      600

gaagttgata caaatccgct gttaggtgca ggtaaatttg caaccgatcc ggcagttacc      660

ctggcacatg aactgattca tgccggtcat cgtctgtatg gtattgcaat taatccgaac      720

cgtgtgttca aagtgaatac caacgcatat tatgaaatga gcggtctgga agtgtcattt      780

gaagaactgc gtacctttgg tggtcatgat gccaaattta tcgatagcct gcaagaaaat      840

gaatttcgcc tgtactacta taacaaattc aaggatattg cgagcaccct gaataaagcc      900

aaaagcattg ttggcaccac cgcaagcctg cagtatatga aaaatgtgtt taaagaaaaa      960

tatctgctga gcgaagatac cagcggtaaa tttagcgttg acaaactgaa attcgataaa     1020

ctgtacaaga tgctgaccga gatttatacc gaagataact tcgtgaagtt tttcaaagtg     1080

ctgaaccgca aaacctacct gaactttgat aaagccgtgt tcaaaatcaa catcgtgccg     1140

aaagtgaact ataccatcta tgatggtttt aacctgcgca ataccaatct ggcagcaaac     1200

tttaatggtc agaacaccga aatcaacaac atgaacttta ccaaactgaa gaacttcacc     1260

ggtctgttcg aattttacaa actgctgtgt gttcgtggca ttattaccag caaaaccaaa     1320

agtctggata aaggctacaa taaagccctg aatgatctgt gcattaaggt gaataattgg     1380

gacctgtttt ttagcccgag cgaggataat ttcaccaacg atctgaacaa aggcgaagaa     1440

attaccagcg ataccaatat tgaagcagcc gaagaaaaca ttagcctgga tctgattcag     1500

cagtattatc tgaccttcaa cttcgataat gagccggaaa atatcagcat tgaaaacctg     1560

agcagcgata ttattggcca gctggaactg atgccgaata ttgaacgttt tccgaacggc     1620

aaaaaatacg agctggataa atacaccatg ttccattatc tgcgtgccca agaatttgaa     1680

catggtaaaa gccgtattgc actgaccaat agcgttaatg aagcactgct gaacccgagc     1740

cgtgtttata ccttttttag cagcgattac gtgaaaaagg ttaacaaagc aaccgaagca     1800

gccatgtttt taggttgggt tgaacagctg gtttatgatt tcaccgatga aaccagcgaa     1860

gttagcacca ccgataaaat tgcagatatt accatcatca tcccgtatat cggtccggca     1920

ctgaatattg gcaatatgct gtataaagac gattttgtgg gtgccctgat ttttagcggt     1980

gcagttattc tgctggaatt tattccggaa attgccattc cggttctggg cacctttgca     2040

ctggtgagct atattgcaaa taaagttctg accgtgcaga ccatcgataa tgcactgagc     2100

aaacgtaacg aaaaatggga tgaagtgtac aagtatatcg tgaccaattg gctggcaaaa     2160

gttaacaccc agattgacct gattcgcaag aagatgaaag aagcactgga aaatcaggca     2220

gaagcaacca aagccattat caactatcag tataaccagt acaccgaaga agagaaaaat     2280

aacatcaact tcaacatcga cgatctgtcc agcaaactga acgaaagcat caacaaagcc     2340

atgattaaca ttaacaaatt tctgaaccag tgcagcgtga gctatctgat gaatagcatg     2400

attccgtatg gtgtgaaacg tctggaagat tttgatgcaa gcctgaaaga tgccctgctg     2460

aaatatatct atgataatcg tggcaccctg attggtcagg ttgatcgtct gaaagataaa     2520

gtgaacaaca ccctgagtac cgatattcct tttcagctga gcaaatatgt ggataatcag     2580

cgtctgctgt caaccgaaaa tctgtatttc cagggtgcaa gtcatcatca ccatcaccac     2640

catcattaa                                                             2649


<210>  20
<211>  882
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide Sequence of rLHN/A (His-tagged)

<400>  20

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly Tyr Asn Lys 
        435                 440                 445             


Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe 
    450                 455                 460                 


Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu 
465                 470                 475                 480 


Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu 
                485                 490                 495     


Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro 
            500                 505                 510         


Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu 
        515                 520                 525             


Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu 
    530                 535                 540                 


Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu 
545                 550                 555                 560 


His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu 
                565                 570                 575     


Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys 
            580                 585                 590         


Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu 
        595                 600                 605             


Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr 
    610                 615                 620                 


Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala 
625                 630                 635                 640 


Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu 
                645                 650                 655     


Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala 
            660                 665                 670         


Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys 
        675                 680                 685             


Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu 
    690                 695                 700                 


Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys 
705                 710                 715                 720 


Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu 
                725                 730                 735     


Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn 
            740                 745                 750         


Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp 
        755                 760                 765             


Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile 
    770                 775                 780                 


Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met 
785                 790                 795                 800 


Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys 
                805                 810                 815     


Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly 
            820                 825                 830         


Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp 
        835                 840                 845             


Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser 
    850                 855                 860                 


Thr Glu Asn Leu Tyr Phe Gln Gly Ala Ser His His His His His His 
865                 870                 875                 880 


His His 
        


<210>  21
<211>  1323
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide Sequence of rHC/A (His-tagged)

<400>  21
atgcatcatc accatcacca cgaaaatcta tacttccaag gaaaaaacat catcaatact       60

agcattctga acctgcgtta cgagagcaat catctgattg atctgagccg ttatgcaagc      120

aagatcaaca tcggtagcaa ggtcaatttt gacccgatcg ataagaacca gatccagctg      180

tttaatctgg aatcgagcaa aattgaggtt atcctgaaaa acgccattgt ctacaactcc      240

atgtacgaga atttctccac cagcttctgg attcgcatcc cgaaatactt caacagcatt      300

agcctgaaca acgagtatac tatcatcaac tgtatggaga acaacagcgg ttggaaggtg      360

tctctgaact atggtgagat catttggacc ttgcaggaca cccaagagat caagcagcgc      420

gtcgtgttca agtactctca aatgatcaac atttccgatt acattaatcg ttggatcttc      480

gtgaccatta cgaataaccg tctgaataac agcaagattt acatcaatgg tcgcttgatc      540

gatcagaaac cgattagcaa cctgggtaat atccacgcaa gcaacaacat tatgttcaaa      600

ttggacggtt gccgcgatac ccatcgttat atctggatca agtatttcaa cctgtttgat      660

aaagaactga atgagaagga gatcaaagat ttgtatgaca accaatctaa cagcggcatt      720

ttgaaggact tctggggcga ttatctgcaa tacgataagc cgtactatat gctgaacctg      780

tatgatccga acaaatatgt ggatgtcaat aatgtgggta ttcgtggtta catgtatttg      840

aagggtccgc gtggcagcgt tatgacgacc aacatttacc tgaactctag cctgtaccgt      900

ggtacgaaat tcatcattaa gaaatatgcc agcggcaaca aagataacat tgtgcgtaat      960

aacgatcgtg tctacatcaa cgtggtcgtg aagaataaag agtaccgtct ggcgaccaac     1020

gcttcgcagg cgggtgttga gaaaattctg agcgcgttgg agatccctga tgtcggtaat     1080

ctgagccaag tcgtggttat gaagagcaag aacgaccagg gtatcactaa caagtgcaag     1140

atgaacctgc aagacaacaa tggtaacgac atcggcttta ttggtttcca ccagttcaac     1200

aatattgcta aactggtagc gagcaattgg tacaatcgtc agattgagcg cagcagccgt     1260

actttgggct gtagctggga gtttatcccg gtcgatgatg gttggggcga acgtccgctg     1320

taa                                                                   1323


<210>  22
<211>  440
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide Sequence of rHC/A (His-tagged)

<400>  22

Met His His His His His His Glu Asn Leu Tyr Phe Gln Gly Lys Asn 
1               5                   10                  15      


Ile Ile Asn Thr Ser Ile Leu Asn Leu Arg Tyr Glu Ser Asn His Leu 
            20                  25                  30          


Ile Asp Leu Ser Arg Tyr Ala Ser Lys Ile Asn Ile Gly Ser Lys Val 
        35                  40                  45              


Asn Phe Asp Pro Ile Asp Lys Asn Gln Ile Gln Leu Phe Asn Leu Glu 
    50                  55                  60                  


Ser Ser Lys Ile Glu Val Ile Leu Lys Asn Ala Ile Val Tyr Asn Ser 
65                  70                  75                  80  


Met Tyr Glu Asn Phe Ser Thr Ser Phe Trp Ile Arg Ile Pro Lys Tyr 
                85                  90                  95      


Phe Asn Ser Ile Ser Leu Asn Asn Glu Tyr Thr Ile Ile Asn Cys Met 
            100                 105                 110         


Glu Asn Asn Ser Gly Trp Lys Val Ser Leu Asn Tyr Gly Glu Ile Ile 
        115                 120                 125             


Trp Thr Leu Gln Asp Thr Gln Glu Ile Lys Gln Arg Val Val Phe Lys 
    130                 135                 140                 


Tyr Ser Gln Met Ile Asn Ile Ser Asp Tyr Ile Asn Arg Trp Ile Phe 
145                 150                 155                 160 


Val Thr Ile Thr Asn Asn Arg Leu Asn Asn Ser Lys Ile Tyr Ile Asn 
                165                 170                 175     


Gly Arg Leu Ile Asp Gln Lys Pro Ile Ser Asn Leu Gly Asn Ile His 
            180                 185                 190         


Ala Ser Asn Asn Ile Met Phe Lys Leu Asp Gly Cys Arg Asp Thr His 
        195                 200                 205             


Arg Tyr Ile Trp Ile Lys Tyr Phe Asn Leu Phe Asp Lys Glu Leu Asn 
    210                 215                 220                 


Glu Lys Glu Ile Lys Asp Leu Tyr Asp Asn Gln Ser Asn Ser Gly Ile 
225                 230                 235                 240 


Leu Lys Asp Phe Trp Gly Asp Tyr Leu Gln Tyr Asp Lys Pro Tyr Tyr 
                245                 250                 255     


Met Leu Asn Leu Tyr Asp Pro Asn Lys Tyr Val Asp Val Asn Asn Val 
            260                 265                 270         


Gly Ile Arg Gly Tyr Met Tyr Leu Lys Gly Pro Arg Gly Ser Val Met 
        275                 280                 285             


Thr Thr Asn Ile Tyr Leu Asn Ser Ser Leu Tyr Arg Gly Thr Lys Phe 
    290                 295                 300                 


Ile Ile Lys Lys Tyr Ala Ser Gly Asn Lys Asp Asn Ile Val Arg Asn 
305                 310                 315                 320 


Asn Asp Arg Val Tyr Ile Asn Val Val Val Lys Asn Lys Glu Tyr Arg 
                325                 330                 335     


Leu Ala Thr Asn Ala Ser Gln Ala Gly Val Glu Lys Ile Leu Ser Ala 
            340                 345                 350         


Leu Glu Ile Pro Asp Val Gly Asn Leu Ser Gln Val Val Val Met Lys 
        355                 360                 365             


Ser Lys Asn Asp Gln Gly Ile Thr Asn Lys Cys Lys Met Asn Leu Gln 
    370                 375                 380                 


Asp Asn Asn Gly Asn Asp Ile Gly Phe Ile Gly Phe His Gln Phe Asn 
385                 390                 395                 400 


Asn Ile Ala Lys Leu Val Ala Ser Asn Trp Tyr Asn Arg Gln Ile Glu 
                405                 410                 415     


Arg Ser Ser Arg Thr Leu Gly Cys Ser Trp Glu Phe Ile Pro Val Asp 
            420                 425                 430         


Asp Gly Trp Gly Glu Arg Pro Leu 
        435                 440 


<210>  23
<211>  1326
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide Sequence of rLC/A (His-tagged)

<400>  23
atgccgtttg tgaacaagca gttcaactat aaagatccgg ttaatggtgt ggatatcgcc       60

tatatcaaaa ttccgaatgc aggtcagatg cagccggtta aagcctttaa aatccataac      120

aaaatttggg tgattccgga acgtgatacc tttaccaatc cggaagaagg tgatctgaat      180

ccgcctccgg aagcaaaaca ggttccggtt agctattatg atagcaccta tctgagcacc      240

gataacgaga aagataacta tctgaaaggt gtgaccaaac tgtttgaacg catttatagt      300

accgatctgg gtcgtatgct gctgaccagc attgttcgtg gtattccgtt ttggggtggt      360

agcaccattg ataccgaact gaaagttatt gacaccaact gcattaatgt gattcagccg      420

gatggtagct atcgtagcga agaactgaat ctggttatta ttggtccgag cgcagatatc      480

attcagtttg aatgtaaatc ctttggccac gaagttctga atctgacccg taatggttat      540

ggtagtaccc agtatattcg tttcagtccg gattttacct ttggctttga agaaagcctg      600

gaagttgata caaatccgct gttaggtgca ggtaaatttg caaccgatcc ggcagttacc      660

ctggcacatg aactgattca tgccggtcat cgtctgtatg gtattgcaat taatccgaac      720

cgtgtgttca aagtgaatac caacgcatat tatgaaatga gcggtctgga agtgtcattt      780

gaagaactgc gtacctttgg tggtcatgat gccaaattta tcgatagcct gcaagaaaat      840

gaatttcgcc tgtactacta taacaaattc aaggatattg cgagcaccct gaataaagcc      900

aaaagcattg ttggcaccac cgcaagcctg cagtatatga aaaatgtgtt taaagaaaaa      960

tatctgctga gcgaagatac cagcggtaaa tttagcgttg acaaactgaa attcgataaa     1020

ctgtacaaga tgctgaccga gatttatacc gaagataact tcgtgaagtt tttcaaagtg     1080

ctgaaccgca aaacctacct gaactttgat aaagccgtgt tcaaaatcaa catcgtgccg     1140

aaagtgaact ataccatcta tgatggtttt aacctgcgca ataccaatct ggcagcaaac     1200

tttaatggtc agaacaccga aatcaacaac atgaacttta ccaaactgaa gaacttcacc     1260

ggtctgtttg aagagaatct gtatttccag ggtgcaagtc atcatcacca tcaccaccat     1320

cattaa                                                                1326


<210>  24
<211>  441
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide Sequence of rLC/A (His-tagged)

<400>  24

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Glu Asn Leu Tyr Phe Gln Gly Ala 
            420                 425                 430         


Ser His His His His His His His His 
        435                 440     


<210>  25
<211>  3918
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide Sequence of rBoNT/FA(0) (His-tagged)

<400>  25
atgccggttg tgattaacag cttcaattat gatgatccgg tgaacgataa caccatcatt       60

tatatccgtc cgccttatta tgaaaccagc aacacctatt tcaaagcctt ccagattatg      120

gataacgtgt ggattattcc ggaacgttat cgtctgggta ttgatccgag cctgtttaat      180

ccgcctgtta gcctgaaagc aggtagtgat ggttattttg atccgaatta tctgagcacc      240

aacaccgaga aaaacaaata cctgcagatt atgatcaagc tgttcaaacg cattaatagc      300

aaaccggcag gtcagattct gctggaagaa atcaaaaatg caattccgta tctgggcaac      360

agctataccc aagaagaaca gtttaccacc aataatcgta ccgtgagctt taatgttaaa      420

ctggccaatg gtaatatcgt tcagcagatg gcaaatctga ttatttgggg tccgggtcct      480

gatctgacca caaataaaac cggtggtatc atctatagcc cgtatcagag catggaagca      540

accccgtata aagatggttt tggtagcatt atgaccgtgg aatttagtcc ggaatatgca      600

accgccttta acgatatttc aattgcaagc catagtccgt cgctgtttat caaagatccg      660

gcactgattc tgatgcacca gctgatttat gttctgcatg gtctgtatgg cacctatatc      720

accgaataca aaattacccc gaatgtggtt cagagctata tgaaagttac caaaccgatt      780

accagcgcag aatttctgac ctttggtggt cgtgatcgca atattgttcc gcagagcatt      840

cagagccagc tgtataacaa agttctgagc gattataaac gtattgccag ccgtctgaat      900

aaagttaata ccgcaaccgc actgatcaac atcgatgaat tcaaaaacct gtacgagtgg      960

aaataccagt ttgccaaaga tagcaatggt gtgtatagcg tggatctgaa caaatttgag     1020

cagctgtaca aaaaaatcta tagcttcacc gaattcaacc tggcctatga gtttaaaatc     1080

aaaacccgtc tgggttatct ggccgaaaat tttggtccgt tttatctgcc gaatctgctg     1140

gatgatagca tttataccga agtggatggt tttaacattg gtgcactgag cattaactat     1200

cagggtcaga atattggcag cgatatcaac agcatcaaaa aactgcaagg tcagggtgtt     1260

gttagccgtg ttgttcgtct gtgtagcaat agcaatacca aaaacagcct gtgcattacc     1320

gttaataatc gcgacctgtt ttttatcgca agccaagaaa gctatggcga gaataccatt     1380

aacacctata aagagattga cgataccacc acactggatc cgagctttga agatattctg     1440

gataaagtga tcctgaactt caacgaacag gttattccgc agatgccgaa tcgtaatgtt     1500

agcaccgata ttcagaaaga caactacatc ccgaaatacg attataaccg caccgacatt     1560

atcgatagct atgaagttgg tcgcaactac aacacctttt tctatctgaa tgcccagaaa     1620

tttagcccga acgaaagcaa tattaccctg accagcagct ttgatacagg tctgttagaa     1680

ggtagcaaag tgtatacctt tttcagcagc gatttcatta acaacatcaa caaaccggtt     1740

caggccctgc tgtttattga atgggttaaa caggtgattc gcgattttac caccgaagca     1800

accaaaacct caaccgttga taaactgaaa gatattagcc tggtggtgcc gtatattggt     1860

ctggcactga atattggtga tgagatctac aaacagcatt ttgcagaagc agttgaactg     1920

gttggtgcag gtctgctgct ggaattttca ccggaatttc ttattccgac gctgctgatt     1980

tttaccatca aaggttatct gaccggtagc attcgcgata aagacaaaat cattaaaacc     2040

ctggataacg ccctgaatgt tcgtgatcag aaatggaaag aactgtatcg ttgggttgtt     2100

agcaaatggc tgaccaccat taatacgcag ttcaacaaac gcaaagaaca aatgtacaaa     2160

gccctgaaaa atcaggccac cgccattaaa aagatcatcg agaacaaata taacaactat     2220

accaccgatg aaaaaagcaa gatcgatagc agctataaca tcaacgaaat tgaacgcacc     2280

ctgaacgaaa aaatcaatct ggccatgaaa aacatcgagc agtttattac cgaaagcagc     2340

attgcctatc tgatcaatat catcaacaac gaaacgatcc agaaactgaa aagctatgat     2400

gacctggttc gtcgttatct gctgggttat attcgtaatc atagcagcat tctgggcaat     2460

agcgttgaag aactgaattc caaagtgaac aaccatctgg ataatggcat tccgtttgaa     2520

ctgagcagtt ataccaatga tagcctgctg atccgctact tcaataaaaa ctatggcgaa     2580

ctgaagtaca actgcattct gaacatcaaa tatgagatgg atcgtgacaa actggttgat     2640

agcagcggtt atcgtagccg tatcaatatt ggtacaggcg tcaaatttag cgagatcgat     2700

aaaaatcaag tgcagctgag caatctggaa tccagcaaaa ttgaagtcat tctgaataac     2760

ggcgtcatct ataacagcat gtatgaaaac ttttcgacca gcttttggat tcgcattccg     2820

aaatactttc gcaacatcaa taacgagtac aagatcatca gctgtatgca gaataatagc     2880

ggttgggaag tgagcctgaa ttttagcaat atgaactcga aaatcatctg gaccctgcag     2940

gataccgaag gtatcaaaaa aaccgttgtg tttcagtaca cccagaacat taacattagc     3000

gactatatca accgctggat ctttgtgacc attacaaata atcgtctgag caacagcaaa     3060

atctacatta atggtcgcct gatcaacgaa gaaagcatta gcgatctggg taatatccat     3120

gccagcaaca acattatgtt taaactggat ggttgccgtg atccgcatcg ttatatctgg     3180

attaaatact ttaacctgtt tgacaaagag ctgaacaaga aagaaattaa agatctgtac     3240

gacaaccaga gcaatagcgg tattctgaaa gatttctggg gtgattatct gcagtatgac     3300

aaaccgtatt atatgctgaa tctgtatgac ccgaataagt atctggatgt gaataatgtt     3360

ggcatccgtg gctatatgta tctgaaaggt ccgcgtggtc gtattgtgac caccaacatt     3420

tatctgaata gcaccctgta tatgggcacc aaattcatca ttaagaaata tgccagcggc     3480

aacaaagata acattgtgcg taataatgat cgcgtgtata ttaacgtggt ggtgaagaat     3540

aaagaatatc gcctggcaac caatgcaagc caggcaggcg ttgaaaaaat tctgagcgca     3600

gttgaaatcc cggatgttgg taatctgagc caggttgttg tgatgaaaag cgaaaatgat     3660

cagggcattc gcaacaagtg taaaatgaat ctgcaagaca ataacggcaa cgatattggc     3720

tttatcggct ttcaccagtt taataacatt gcaaaactgg tggccagcaa ctggtataac     3780

cgtcagattg gtaaagcaag ccgtaccttt ggttgtagct gggaatttat cccggttgat     3840

gatggttggg gtgaaagcag cctggaaaat ctgtatttcc agggtgccag tcatcatcac     3900

caccatcacc atcactga                                                   3918


<210>  26
<211>  1305
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide Sequence of rBoNT/FA(0) (His-tagged)

<400>  26

Met Pro Val Val Ile Asn Ser Phe Asn Tyr Asp Asp Pro Val Asn Asp 
1               5                   10                  15      


Asn Thr Ile Ile Tyr Ile Arg Pro Pro Tyr Tyr Glu Thr Ser Asn Thr 
            20                  25                  30          


Tyr Phe Lys Ala Phe Gln Ile Met Asp Asn Val Trp Ile Ile Pro Glu 
        35                  40                  45              


Arg Tyr Arg Leu Gly Ile Asp Pro Ser Leu Phe Asn Pro Pro Val Ser 
    50                  55                  60                  


Leu Lys Ala Gly Ser Asp Gly Tyr Phe Asp Pro Asn Tyr Leu Ser Thr 
65                  70                  75                  80  


Asn Thr Glu Lys Asn Lys Tyr Leu Gln Ile Met Ile Lys Leu Phe Lys 
                85                  90                  95      


Arg Ile Asn Ser Lys Pro Ala Gly Gln Ile Leu Leu Glu Glu Ile Lys 
            100                 105                 110         


Asn Ala Ile Pro Tyr Leu Gly Asn Ser Tyr Thr Gln Glu Glu Gln Phe 
        115                 120                 125             


Thr Thr Asn Asn Arg Thr Val Ser Phe Asn Val Lys Leu Ala Asn Gly 
    130                 135                 140                 


Asn Ile Val Gln Gln Met Ala Asn Leu Ile Ile Trp Gly Pro Gly Pro 
145                 150                 155                 160 


Asp Leu Thr Thr Asn Lys Thr Gly Gly Ile Ile Tyr Ser Pro Tyr Gln 
                165                 170                 175     


Ser Met Glu Ala Thr Pro Tyr Lys Asp Gly Phe Gly Ser Ile Met Thr 
            180                 185                 190         


Val Glu Phe Ser Pro Glu Tyr Ala Thr Ala Phe Asn Asp Ile Ser Ile 
        195                 200                 205             


Ala Ser His Ser Pro Ser Leu Phe Ile Lys Asp Pro Ala Leu Ile Leu 
    210                 215                 220                 


Met His Gln Leu Ile Tyr Val Leu His Gly Leu Tyr Gly Thr Tyr Ile 
225                 230                 235                 240 


Thr Glu Tyr Lys Ile Thr Pro Asn Val Val Gln Ser Tyr Met Lys Val 
                245                 250                 255     


Thr Lys Pro Ile Thr Ser Ala Glu Phe Leu Thr Phe Gly Gly Arg Asp 
            260                 265                 270         


Arg Asn Ile Val Pro Gln Ser Ile Gln Ser Gln Leu Tyr Asn Lys Val 
        275                 280                 285             


Leu Ser Asp Tyr Lys Arg Ile Ala Ser Arg Leu Asn Lys Val Asn Thr 
    290                 295                 300                 


Ala Thr Ala Leu Ile Asn Ile Asp Glu Phe Lys Asn Leu Tyr Glu Trp 
305                 310                 315                 320 


Lys Tyr Gln Phe Ala Lys Asp Ser Asn Gly Val Tyr Ser Val Asp Leu 
                325                 330                 335     


Asn Lys Phe Glu Gln Leu Tyr Lys Lys Ile Tyr Ser Phe Thr Glu Phe 
            340                 345                 350         


Asn Leu Ala Tyr Glu Phe Lys Ile Lys Thr Arg Leu Gly Tyr Leu Ala 
        355                 360                 365             


Glu Asn Phe Gly Pro Phe Tyr Leu Pro Asn Leu Leu Asp Asp Ser Ile 
    370                 375                 380                 


Tyr Thr Glu Val Asp Gly Phe Asn Ile Gly Ala Leu Ser Ile Asn Tyr 
385                 390                 395                 400 


Gln Gly Gln Asn Ile Gly Ser Asp Ile Asn Ser Ile Lys Lys Leu Gln 
                405                 410                 415     


Gly Gln Gly Val Val Ser Arg Val Val Arg Leu Cys Ser Asn Ser Asn 
            420                 425                 430         


Thr Lys Asn Ser Leu Cys Ile Thr Val Asn Asn Arg Asp Leu Phe Phe 
        435                 440                 445             


Ile Ala Ser Gln Glu Ser Tyr Gly Glu Asn Thr Ile Asn Thr Tyr Lys 
    450                 455                 460                 


Glu Ile Asp Asp Thr Thr Thr Leu Asp Pro Ser Phe Glu Asp Ile Leu 
465                 470                 475                 480 


Asp Lys Val Ile Leu Asn Phe Asn Glu Gln Val Ile Pro Gln Met Pro 
                485                 490                 495     


Asn Arg Asn Val Ser Thr Asp Ile Gln Lys Asp Asn Tyr Ile Pro Lys 
            500                 505                 510         


Tyr Asp Tyr Asn Arg Thr Asp Ile Ile Asp Ser Tyr Glu Val Gly Arg 
        515                 520                 525             


Asn Tyr Asn Thr Phe Phe Tyr Leu Asn Ala Gln Lys Phe Ser Pro Asn 
    530                 535                 540                 


Glu Ser Asn Ile Thr Leu Thr Ser Ser Phe Asp Thr Gly Leu Leu Glu 
545                 550                 555                 560 


Gly Ser Lys Val Tyr Thr Phe Phe Ser Ser Asp Phe Ile Asn Asn Ile 
                565                 570                 575     


Asn Lys Pro Val Gln Ala Leu Leu Phe Ile Glu Trp Val Lys Gln Val 
            580                 585                 590         


Ile Arg Asp Phe Thr Thr Glu Ala Thr Lys Thr Ser Thr Val Asp Lys 
        595                 600                 605             


Leu Lys Asp Ile Ser Leu Val Val Pro Tyr Ile Gly Leu Ala Leu Asn 
    610                 615                 620                 


Ile Gly Asp Glu Ile Tyr Lys Gln His Phe Ala Glu Ala Val Glu Leu 
625                 630                 635                 640 


Val Gly Ala Gly Leu Leu Leu Glu Phe Ser Pro Glu Phe Leu Ile Pro 
                645                 650                 655     


Thr Leu Leu Ile Phe Thr Ile Lys Gly Tyr Leu Thr Gly Ser Ile Arg 
            660                 665                 670         


Asp Lys Asp Lys Ile Ile Lys Thr Leu Asp Asn Ala Leu Asn Val Arg 
        675                 680                 685             


Asp Gln Lys Trp Lys Glu Leu Tyr Arg Trp Val Val Ser Lys Trp Leu 
    690                 695                 700                 


Thr Thr Ile Asn Thr Gln Phe Asn Lys Arg Lys Glu Gln Met Tyr Lys 
705                 710                 715                 720 


Ala Leu Lys Asn Gln Ala Thr Ala Ile Lys Lys Ile Ile Glu Asn Lys 
                725                 730                 735     


Tyr Asn Asn Tyr Thr Thr Asp Glu Lys Ser Lys Ile Asp Ser Ser Tyr 
            740                 745                 750         


Asn Ile Asn Glu Ile Glu Arg Thr Leu Asn Glu Lys Ile Asn Leu Ala 
        755                 760                 765             


Met Lys Asn Ile Glu Gln Phe Ile Thr Glu Ser Ser Ile Ala Tyr Leu 
    770                 775                 780                 


Ile Asn Ile Ile Asn Asn Glu Thr Ile Gln Lys Leu Lys Ser Tyr Asp 
785                 790                 795                 800 


Asp Leu Val Arg Arg Tyr Leu Leu Gly Tyr Ile Arg Asn His Ser Ser 
                805                 810                 815     


Ile Leu Gly Asn Ser Val Glu Glu Leu Asn Ser Lys Val Asn Asn His 
            820                 825                 830         


Leu Asp Asn Gly Ile Pro Phe Glu Leu Ser Ser Tyr Thr Asn Asp Ser 
        835                 840                 845             


Leu Leu Ile Arg Tyr Phe Asn Lys Asn Tyr Gly Glu Leu Lys Tyr Asn 
    850                 855                 860                 


Cys Ile Leu Asn Ile Lys Tyr Glu Met Asp Arg Asp Lys Leu Val Asp 
865                 870                 875                 880 


Ser Ser Gly Tyr Arg Ser Arg Ile Asn Ile Gly Thr Gly Val Lys Phe 
                885                 890                 895     


Ser Glu Ile Asp Lys Asn Gln Val Gln Leu Ser Asn Leu Glu Ser Ser 
            900                 905                 910         


Lys Ile Glu Val Ile Leu Asn Asn Gly Val Ile Tyr Asn Ser Met Tyr 
        915                 920                 925             


Glu Asn Phe Ser Thr Ser Phe Trp Ile Arg Ile Pro Lys Tyr Phe Arg 
    930                 935                 940                 


Asn Ile Asn Asn Glu Tyr Lys Ile Ile Ser Cys Met Gln Asn Asn Ser 
945                 950                 955                 960 


Gly Trp Glu Val Ser Leu Asn Phe Ser Asn Met Asn Ser Lys Ile Ile 
                965                 970                 975     


Trp Thr Leu Gln Asp Thr Glu Gly Ile Lys Lys Thr Val Val Phe Gln 
            980                 985                 990         


Tyr Thr Gln Asn Ile Asn Ile Ser  Asp Tyr Ile Asn Arg  Trp Ile Phe 
        995                 1000                 1005             


Val Thr  Ile Thr Asn Asn Arg  Leu Ser Asn Ser Lys  Ile Tyr Ile 
    1010                 1015                 1020             


Asn Gly  Arg Leu Ile Asn Glu  Glu Ser Ile Ser Asp  Leu Gly Asn 
    1025                 1030                 1035             


Ile His  Ala Ser Asn Asn Ile  Met Phe Lys Leu Asp  Gly Cys Arg 
    1040                 1045                 1050             


Asp Pro  His Arg Tyr Ile Trp  Ile Lys Tyr Phe Asn  Leu Phe Asp 
    1055                 1060                 1065             


Lys Glu  Leu Asn Lys Lys Glu  Ile Lys Asp Leu Tyr  Asp Asn Gln 
    1070                 1075                 1080             


Ser Asn  Ser Gly Ile Leu Lys  Asp Phe Trp Gly Asp  Tyr Leu Gln 
    1085                 1090                 1095             


Tyr Asp  Lys Pro Tyr Tyr Met  Leu Asn Leu Tyr Asp  Pro Asn Lys 
    1100                 1105                 1110             


Tyr Leu  Asp Val Asn Asn Val  Gly Ile Arg Gly Tyr  Met Tyr Leu 
    1115                 1120                 1125             


Lys Gly  Pro Arg Gly Arg Ile  Val Thr Thr Asn Ile  Tyr Leu Asn 
    1130                 1135                 1140             


Ser Thr  Leu Tyr Met Gly Thr  Lys Phe Ile Ile Lys  Lys Tyr Ala 
    1145                 1150                 1155             


Ser Gly  Asn Lys Asp Asn Ile  Val Arg Asn Asn Asp  Arg Val Tyr 
    1160                 1165                 1170             


Ile Asn  Val Val Val Lys Asn  Lys Glu Tyr Arg Leu  Ala Thr Asn 
    1175                 1180                 1185             


Ala Ser  Gln Ala Gly Val Glu  Lys Ile Leu Ser Ala  Val Glu Ile 
    1190                 1195                 1200             


Pro Asp  Val Gly Asn Leu Ser  Gln Val Val Val Met  Lys Ser Glu 
    1205                 1210                 1215             


Asn Asp  Gln Gly Ile Arg Asn  Lys Cys Lys Met Asn  Leu Gln Asp 
    1220                 1225                 1230             


Asn Asn  Gly Asn Asp Ile Gly  Phe Ile Gly Phe His  Gln Phe Asn 
    1235                 1240                 1245             


Asn Ile  Ala Lys Leu Val Ala  Ser Asn Trp Tyr Asn  Arg Gln Ile 
    1250                 1255                 1260             


Gly Lys  Ala Ser Arg Thr Phe  Gly Cys Ser Trp Glu  Phe Ile Pro 
    1265                 1270                 1275             


Val Asp  Asp Gly Trp Gly Glu  Ser Ser Leu Glu Asn  Leu Tyr Phe 
    1280                 1285                 1290             


Gln Gly  Ala Ser His His His  His His His His His  
    1295                 1300                 1305 


<210>  27
<211>  2634
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide Sequence of rLHN/FA (His-tagged)

<400>  27
atgccggttg tgattaacag cttcaattat gatgatccgg tgaacgataa caccatcatt       60

tatatccgtc cgccttatta tgaaaccagc aacacctatt tcaaagcctt ccagattatg      120

gataacgtgt ggattattcc ggaacgttat cgtctgggta ttgatccgag cctgtttaat      180

ccgcctgtta gcctgaaagc aggtagtgat ggttattttg atccgaatta tctgagcacc      240

aacaccgaga aaaacaaata cctgcagatt atgatcaagc tgttcaaacg cattaatagc      300

aaaccggcag gtcagattct gctggaagaa atcaaaaatg caattccgta tctgggcaac      360

agctataccc aagaagaaca gtttaccacc aataatcgta ccgtgagctt taatgttaaa      420

ctggccaatg gtaatatcgt tcagcagatg gcaaatctga ttatttgggg tccgggtcct      480

gatctgacca caaataaaac cggtggtatc atctatagcc cgtatcagag catggaagca      540

accccgtata aagatggttt tggtagcatt atgaccgtgg aatttagtcc ggaatatgca      600

accgccttta acgatatttc aattgcaagc catagtccgt cgctgtttat caaagatccg      660

gcactgattc tgatgcatga actgattcat gttctgcatg gtctgtatgg cacctatatt      720

accgaataca aaattacccc gaatgtggtg cagagctata tgaaagttac caaaccgatt      780

accagcgcag aatttctgac ctttggtggt cgtgatcgca atattgttcc gcagagcatt      840

cagagccagc tgtataacaa agttctgagc gattataaac gtattgccag ccgtctgaat      900

aaagttaata ccgcaaccgc actgatcaac atcgatgaat tcaaaaacct gtacgagtgg      960

aaataccagt ttgccaaaga tagcaatggt gtgtatagcg tggatctgaa caaatttgag     1020

cagctgtaca aaaaaatcta tagcttcacc gaattcaacc tggcctatga gtttaaaatc     1080

aaaacccgtc tgggttatct ggccgaaaat tttggtccgt tttatctgcc gaatctgctg     1140

gatgatagca tttataccga agtggatggt tttaacattg gtgcactgag cattaactat     1200

cagggtcaga atattggcag cgatatcaac agcatcaaaa aactgcaagg tcagggtgtt     1260

gttagccgtg ttgttcgtct gtgtagcaat agcaatacca aaaacagcct gtgcattacc     1320

gttaataatc gcgacctgtt ttttatcgca agccaagaaa gctatggcga gaataccatt     1380

aacacctata aagagattga cgataccacc acactggatc cgagctttga agatattctg     1440

gataaagtga tcctgaactt caacgaacag gttattccgc agatgccgaa tcgtaatgtt     1500

agcaccgata ttcagaaaga caactacatc ccgaaatacg attataaccg caccgacatt     1560

atcgatagct atgaagttgg tcgcaactac aacacctttt tctatctgaa tgcccagaaa     1620

tttagcccga acgaaagcaa tattaccctg accagcagct ttgatacagg tctgttagaa     1680

ggtagcaaag tgtatacctt tttcagcagc gatttcatta acaacatcaa caaaccggtt     1740

caggccctgc tgtttattga atgggttaaa caggtgattc gcgattttac caccgaagca     1800

accaaaacct caaccgttga taaactgaaa gatattagcc tggtggtgcc gtatattggt     1860

ctggcactga atattggtga tgagatctac aaacagcatt ttgcagaagc agttgaactg     1920

gttggtgcag gtctgctgct ggaattttca ccggaatttc ttattccgac gctgctgatt     1980

tttaccatca aaggttatct gaccggtagc attcgcgata aagacaaaat cattaaaacc     2040

ctggataacg ccctgaatgt tcgtgatcag aaatggaaag aactgtatcg ttgggttgtt     2100

agcaaatggc tgaccaccat taatacgcag ttcaacaaac gcaaagaaca aatgtacaaa     2160

gccctgaaaa atcaggccac cgccattaaa aagatcatcg agaacaaata taacaactat     2220

accaccgatg aaaaaagcaa gatcgatagc agctataaca tcaacgaaat tgaacgcacc     2280

ctgaacgaaa aaatcaatct ggccatgaaa aacatcgagc agtttattac agaaagcagc     2340

attgcctacc tgatcaatat catcaacaac gaaaccattc agaaactgaa aagctatgat     2400

gacctggttc gtcgttatct gctgggttat attcgtaatc atagcagcat tctgggcaat     2460

agcgttgaag aactgaattc caaagtgaac aaccatctgg ataatggcat tccgtttgaa     2520

ctgagcagtt ataccaatga tagcctgctg atccgctact tcaataaaaa ctatggcgaa     2580

gagaacctgt atttccaggg tgccagtcat catcaccacc atcaccatca ctga           2634


<210>  28
<211>  877
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide Sequence of rLHN/FA (His-tagged)

<400>  28

Met Pro Val Val Ile Asn Ser Phe Asn Tyr Asp Asp Pro Val Asn Asp 
1               5                   10                  15      


Asn Thr Ile Ile Tyr Ile Arg Pro Pro Tyr Tyr Glu Thr Ser Asn Thr 
            20                  25                  30          


Tyr Phe Lys Ala Phe Gln Ile Met Asp Asn Val Trp Ile Ile Pro Glu 
        35                  40                  45              


Arg Tyr Arg Leu Gly Ile Asp Pro Ser Leu Phe Asn Pro Pro Val Ser 
    50                  55                  60                  


Leu Lys Ala Gly Ser Asp Gly Tyr Phe Asp Pro Asn Tyr Leu Ser Thr 
65                  70                  75                  80  


Asn Thr Glu Lys Asn Lys Tyr Leu Gln Ile Met Ile Lys Leu Phe Lys 
                85                  90                  95      


Arg Ile Asn Ser Lys Pro Ala Gly Gln Ile Leu Leu Glu Glu Ile Lys 
            100                 105                 110         


Asn Ala Ile Pro Tyr Leu Gly Asn Ser Tyr Thr Gln Glu Glu Gln Phe 
        115                 120                 125             


Thr Thr Asn Asn Arg Thr Val Ser Phe Asn Val Lys Leu Ala Asn Gly 
    130                 135                 140                 


Asn Ile Val Gln Gln Met Ala Asn Leu Ile Ile Trp Gly Pro Gly Pro 
145                 150                 155                 160 


Asp Leu Thr Thr Asn Lys Thr Gly Gly Ile Ile Tyr Ser Pro Tyr Gln 
                165                 170                 175     


Ser Met Glu Ala Thr Pro Tyr Lys Asp Gly Phe Gly Ser Ile Met Thr 
            180                 185                 190         


Val Glu Phe Ser Pro Glu Tyr Ala Thr Ala Phe Asn Asp Ile Ser Ile 
        195                 200                 205             


Ala Ser His Ser Pro Ser Leu Phe Ile Lys Asp Pro Ala Leu Ile Leu 
    210                 215                 220                 


Met His Glu Leu Ile His Val Leu His Gly Leu Tyr Gly Thr Tyr Ile 
225                 230                 235                 240 


Thr Glu Tyr Lys Ile Thr Pro Asn Val Val Gln Ser Tyr Met Lys Val 
                245                 250                 255     


Thr Lys Pro Ile Thr Ser Ala Glu Phe Leu Thr Phe Gly Gly Arg Asp 
            260                 265                 270         


Arg Asn Ile Val Pro Gln Ser Ile Gln Ser Gln Leu Tyr Asn Lys Val 
        275                 280                 285             


Leu Ser Asp Tyr Lys Arg Ile Ala Ser Arg Leu Asn Lys Val Asn Thr 
    290                 295                 300                 


Ala Thr Ala Leu Ile Asn Ile Asp Glu Phe Lys Asn Leu Tyr Glu Trp 
305                 310                 315                 320 


Lys Tyr Gln Phe Ala Lys Asp Ser Asn Gly Val Tyr Ser Val Asp Leu 
                325                 330                 335     


Asn Lys Phe Glu Gln Leu Tyr Lys Lys Ile Tyr Ser Phe Thr Glu Phe 
            340                 345                 350         


Asn Leu Ala Tyr Glu Phe Lys Ile Lys Thr Arg Leu Gly Tyr Leu Ala 
        355                 360                 365             


Glu Asn Phe Gly Pro Phe Tyr Leu Pro Asn Leu Leu Asp Asp Ser Ile 
    370                 375                 380                 


Tyr Thr Glu Val Asp Gly Phe Asn Ile Gly Ala Leu Ser Ile Asn Tyr 
385                 390                 395                 400 


Gln Gly Gln Asn Ile Gly Ser Asp Ile Asn Ser Ile Lys Lys Leu Gln 
                405                 410                 415     


Gly Gln Gly Val Val Ser Arg Val Val Arg Leu Cys Ser Asn Ser Asn 
            420                 425                 430         


Thr Lys Asn Ser Leu Cys Ile Thr Val Asn Asn Arg Asp Leu Phe Phe 
        435                 440                 445             


Ile Ala Ser Gln Glu Ser Tyr Gly Glu Asn Thr Ile Asn Thr Tyr Lys 
    450                 455                 460                 


Glu Ile Asp Asp Thr Thr Thr Leu Asp Pro Ser Phe Glu Asp Ile Leu 
465                 470                 475                 480 


Asp Lys Val Ile Leu Asn Phe Asn Glu Gln Val Ile Pro Gln Met Pro 
                485                 490                 495     


Asn Arg Asn Val Ser Thr Asp Ile Gln Lys Asp Asn Tyr Ile Pro Lys 
            500                 505                 510         


Tyr Asp Tyr Asn Arg Thr Asp Ile Ile Asp Ser Tyr Glu Val Gly Arg 
        515                 520                 525             


Asn Tyr Asn Thr Phe Phe Tyr Leu Asn Ala Gln Lys Phe Ser Pro Asn 
    530                 535                 540                 


Glu Ser Asn Ile Thr Leu Thr Ser Ser Phe Asp Thr Gly Leu Leu Glu 
545                 550                 555                 560 


Gly Ser Lys Val Tyr Thr Phe Phe Ser Ser Asp Phe Ile Asn Asn Ile 
                565                 570                 575     


Asn Lys Pro Val Gln Ala Leu Leu Phe Ile Glu Trp Val Lys Gln Val 
            580                 585                 590         


Ile Arg Asp Phe Thr Thr Glu Ala Thr Lys Thr Ser Thr Val Asp Lys 
        595                 600                 605             


Leu Lys Asp Ile Ser Leu Val Val Pro Tyr Ile Gly Leu Ala Leu Asn 
    610                 615                 620                 


Ile Gly Asp Glu Ile Tyr Lys Gln His Phe Ala Glu Ala Val Glu Leu 
625                 630                 635                 640 


Val Gly Ala Gly Leu Leu Leu Glu Phe Ser Pro Glu Phe Leu Ile Pro 
                645                 650                 655     


Thr Leu Leu Ile Phe Thr Ile Lys Gly Tyr Leu Thr Gly Ser Ile Arg 
            660                 665                 670         


Asp Lys Asp Lys Ile Ile Lys Thr Leu Asp Asn Ala Leu Asn Val Arg 
        675                 680                 685             


Asp Gln Lys Trp Lys Glu Leu Tyr Arg Trp Val Val Ser Lys Trp Leu 
    690                 695                 700                 


Thr Thr Ile Asn Thr Gln Phe Asn Lys Arg Lys Glu Gln Met Tyr Lys 
705                 710                 715                 720 


Ala Leu Lys Asn Gln Ala Thr Ala Ile Lys Lys Ile Ile Glu Asn Lys 
                725                 730                 735     


Tyr Asn Asn Tyr Thr Thr Asp Glu Lys Ser Lys Ile Asp Ser Ser Tyr 
            740                 745                 750         


Asn Ile Asn Glu Ile Glu Arg Thr Leu Asn Glu Lys Ile Asn Leu Ala 
        755                 760                 765             


Met Lys Asn Ile Glu Gln Phe Ile Thr Glu Ser Ser Ile Ala Tyr Leu 
    770                 775                 780                 


Ile Asn Ile Ile Asn Asn Glu Thr Ile Gln Lys Leu Lys Ser Tyr Asp 
785                 790                 795                 800 


Asp Leu Val Arg Arg Tyr Leu Leu Gly Tyr Ile Arg Asn His Ser Ser 
                805                 810                 815     


Ile Leu Gly Asn Ser Val Glu Glu Leu Asn Ser Lys Val Asn Asn His 
            820                 825                 830         


Leu Asp Asn Gly Ile Pro Phe Glu Leu Ser Ser Tyr Thr Asn Asp Ser 
        835                 840                 845             


Leu Leu Ile Arg Tyr Phe Asn Lys Asn Tyr Gly Glu Glu Asn Leu Tyr 
    850                 855                 860                 


Phe Gln Gly Ala Ser His His His His His His His His 
865                 870                 875         


<210>  29
<211>  1341
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide Sequence of rHC/FA (His-tagged)

<400>  29
atgctgaagt ataactgcat cctgaacatc aaatatgaga tggatcgtga taaactggtt       60

gatagcagcg gttatcgtag ccgtatcaat attggcaccg gtgtgaaatt tagcgagatc      120

gataaaaatc aggtgcagct gagcaatctg gaaagcagca aaattgaagt gattctgaat      180

aacggcgtga tctacaatag catgtatgaa aacttttcga ccagcttctg gattcgcatt      240

ccgaaatact ttcgcaacat caacaacgag tacaagatta tcagctgtat gcagaataat      300

agcggttggg aagttagcct gaatttcagc aatatgaaca gcaaaatcat ttggaccctg      360

caggataccg aaggtatcaa aaaaaccgtt gtgtttcagt acacccagaa cattaacatc      420

agcgattaca ttaaccgctg gatctttgtg accattacca ataatcgtct gagcaacagc      480

aagatctata ttaacggtcg cctgattaac gaagagagca ttagcgatct gggtaatatt      540

catgccagca acaacatcat gtttaaactg gatggttgtc gtgatccgca tcgttatatt      600

tggatcaaat acttcaacct gtttgataaa gaactgaaca aaaaagaaat caaagacctg      660

tatgataacc agagcaatag cggcattctg aaagattttt ggggtgatta tctgcagtat      720

gacaaaccgt attacatgct gaatctgtac gatccgaaca aatatctgga tgtgaataat      780

gtgggtatcc gtggctatat gtatctgaaa ggtccgcgtg gtcgtattgt taccaccaac      840

atttatctga atagcaccct gtatatgggc accaaattca tcattaaaaa gtatgccagc      900

ggcaacaaag ataacattgt gcgtaataat gatcgcgtgt atatcaatgt ggtggtgaag      960

aataaagaat atcgtctggc caccaatgca agccaggcag gcgttgaaaa aattctgagc     1020

gcagttgaaa ttccggatgt tggtaatctg agccaggttg ttgttatgaa aagcgaaaat     1080

gatcagggca ttcgcaacaa atgcaaaatg aatctgcagg acaataacgg caacgatatt     1140

ggttttattg gcttccacca gttcaacaac attgcaaaac tggtggcgag caattggtat     1200

aatcgtcaga ttggtaaagc aagccgtacc tttggttgta gctgggaatt tattccggtt     1260

gatgatggtt ggggtgaaag cagcctggaa aatctgtatt ttcagggtgc aagtcatcat     1320

caccaccatc accatcatta a                                               1341


<210>  30
<211>  446
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide Sequence of rHC/FA (His-tagged)

<400>  30

Met Leu Lys Tyr Asn Cys Ile Leu Asn Ile Lys Tyr Glu Met Asp Arg 
1               5                   10                  15      


Asp Lys Leu Val Asp Ser Ser Gly Tyr Arg Ser Arg Ile Asn Ile Gly 
            20                  25                  30          


Thr Gly Val Lys Phe Ser Glu Ile Asp Lys Asn Gln Val Gln Leu Ser 
        35                  40                  45              


Asn Leu Glu Ser Ser Lys Ile Glu Val Ile Leu Asn Asn Gly Val Ile 
    50                  55                  60                  


Tyr Asn Ser Met Tyr Glu Asn Phe Ser Thr Ser Phe Trp Ile Arg Ile 
65                  70                  75                  80  


Pro Lys Tyr Phe Arg Asn Ile Asn Asn Glu Tyr Lys Ile Ile Ser Cys 
                85                  90                  95      


Met Gln Asn Asn Ser Gly Trp Glu Val Ser Leu Asn Phe Ser Asn Met 
            100                 105                 110         


Asn Ser Lys Ile Ile Trp Thr Leu Gln Asp Thr Glu Gly Ile Lys Lys 
        115                 120                 125             


Thr Val Val Phe Gln Tyr Thr Gln Asn Ile Asn Ile Ser Asp Tyr Ile 
    130                 135                 140                 


Asn Arg Trp Ile Phe Val Thr Ile Thr Asn Asn Arg Leu Ser Asn Ser 
145                 150                 155                 160 


Lys Ile Tyr Ile Asn Gly Arg Leu Ile Asn Glu Glu Ser Ile Ser Asp 
                165                 170                 175     


Leu Gly Asn Ile His Ala Ser Asn Asn Ile Met Phe Lys Leu Asp Gly 
            180                 185                 190         


Cys Arg Asp Pro His Arg Tyr Ile Trp Ile Lys Tyr Phe Asn Leu Phe 
        195                 200                 205             


Asp Lys Glu Leu Asn Lys Lys Glu Ile Lys Asp Leu Tyr Asp Asn Gln 
    210                 215                 220                 


Ser Asn Ser Gly Ile Leu Lys Asp Phe Trp Gly Asp Tyr Leu Gln Tyr 
225                 230                 235                 240 


Asp Lys Pro Tyr Tyr Met Leu Asn Leu Tyr Asp Pro Asn Lys Tyr Leu 
                245                 250                 255     


Asp Val Asn Asn Val Gly Ile Arg Gly Tyr Met Tyr Leu Lys Gly Pro 
            260                 265                 270         


Arg Gly Arg Ile Val Thr Thr Asn Ile Tyr Leu Asn Ser Thr Leu Tyr 
        275                 280                 285             


Met Gly Thr Lys Phe Ile Ile Lys Lys Tyr Ala Ser Gly Asn Lys Asp 
    290                 295                 300                 


Asn Ile Val Arg Asn Asn Asp Arg Val Tyr Ile Asn Val Val Val Lys 
305                 310                 315                 320 


Asn Lys Glu Tyr Arg Leu Ala Thr Asn Ala Ser Gln Ala Gly Val Glu 
                325                 330                 335     


Lys Ile Leu Ser Ala Val Glu Ile Pro Asp Val Gly Asn Leu Ser Gln 
            340                 345                 350         


Val Val Val Met Lys Ser Glu Asn Asp Gln Gly Ile Arg Asn Lys Cys 
        355                 360                 365             


Lys Met Asn Leu Gln Asp Asn Asn Gly Asn Asp Ile Gly Phe Ile Gly 
    370                 375                 380                 


Phe His Gln Phe Asn Asn Ile Ala Lys Leu Val Ala Ser Asn Trp Tyr 
385                 390                 395                 400 


Asn Arg Gln Ile Gly Lys Ala Ser Arg Thr Phe Gly Cys Ser Trp Glu 
                405                 410                 415     


Phe Ile Pro Val Asp Asp Gly Trp Gly Glu Ser Ser Leu Glu Asn Leu 
            420                 425                 430         


Tyr Phe Gln Gly Ala Ser His His His His His His His His 
        435                 440                 445     


<210>  31
<211>  1347
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide Sequence of rLC/FA (His-tagged)

<400>  31
atgccggttg tgattaacag cttcaattat gatgatccgg tgaacgataa caccatcatt       60

tatatccgtc cgccttatta tgaaaccagc aacacctatt tcaaagcctt ccagattatg      120

gataacgtgt ggattattcc ggaacgttat cgtctgggta ttgatccgag cctgtttaat      180

ccgcctgtta gcctgaaagc aggtagtgat ggttattttg atccgaatta tctgagcacc      240

aacaccgaga aaaacaaata cctgcagatt atgatcaagc tgttcaaacg cattaatagc      300

aaaccggcag gtcagattct gctggaagaa atcaaaaatg caattccgta tctgggcaac      360

agctataccc aagaagaaca gtttaccacc aataatcgta ccgtgagctt taatgttaaa      420

ctggccaatg gtaatatcgt tcagcagatg gcaaatctga ttatttgggg tccgggtcct      480

gatctgacca caaataaaac cggtggtatc atctatagcc cgtatcagag catggaagca      540

accccgtata aagatggttt tggtagcatt atgaccgtgg aatttagtcc ggaatatgca      600

accgccttta acgatatttc aattgcaagc catagtccgt cgctgtttat caaagatccg      660

gcactgattc tgatgcatga actgattcat gttctgcatg gtctgtatgg cacctatatt      720

accgaataca aaattacccc gaatgtggtg cagagctata tgaaagttac caaaccgatt      780

accagcgcag aatttctgac ctttggtggt cgtgatcgca atattgttcc gcagagcatt      840

cagagccagc tgtataacaa agttctgagc gattataaac gtattgccag ccgtctgaat      900

aaagttaata ccgcaaccgc actgatcaac atcgatgaat tcaaaaacct gtacgagtgg      960

aaataccagt ttgccaaaga tagcaatggt gtgtatagcg tggatctgaa caaatttgag     1020

cagctgtaca aaaaaatcta tagcttcacc gaattcaacc tggcctatga gtttaaaatc     1080

aaaacccgtc tgggttatct ggccgaaaat tttggtccgt tttatctgcc gaatctgctg     1140

gatgatagca tttataccga agtggatggt tttaacattg gtgcactgag cattaactat     1200

cagggtcaga atattggcag cgatatcaac agcatcaaaa aactgcaagg tcagggtgtt     1260

gttagccgtg ttgttcgtct gtgtagcaat agcgaaaatc tgtattttca gggtgccagt     1320

catcatcacc accatcacca tcactga                                         1347


<210>  32
<211>  448
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide Sequence of rLC/FA (His-tagged)

<400>  32

Met Pro Val Val Ile Asn Ser Phe Asn Tyr Asp Asp Pro Val Asn Asp 
1               5                   10                  15      


Asn Thr Ile Ile Tyr Ile Arg Pro Pro Tyr Tyr Glu Thr Ser Asn Thr 
            20                  25                  30          


Tyr Phe Lys Ala Phe Gln Ile Met Asp Asn Val Trp Ile Ile Pro Glu 
        35                  40                  45              


Arg Tyr Arg Leu Gly Ile Asp Pro Ser Leu Phe Asn Pro Pro Val Ser 
    50                  55                  60                  


Leu Lys Ala Gly Ser Asp Gly Tyr Phe Asp Pro Asn Tyr Leu Ser Thr 
65                  70                  75                  80  


Asn Thr Glu Lys Asn Lys Tyr Leu Gln Ile Met Ile Lys Leu Phe Lys 
                85                  90                  95      


Arg Ile Asn Ser Lys Pro Ala Gly Gln Ile Leu Leu Glu Glu Ile Lys 
            100                 105                 110         


Asn Ala Ile Pro Tyr Leu Gly Asn Ser Tyr Thr Gln Glu Glu Gln Phe 
        115                 120                 125             


Thr Thr Asn Asn Arg Thr Val Ser Phe Asn Val Lys Leu Ala Asn Gly 
    130                 135                 140                 


Asn Ile Val Gln Gln Met Ala Asn Leu Ile Ile Trp Gly Pro Gly Pro 
145                 150                 155                 160 


Asp Leu Thr Thr Asn Lys Thr Gly Gly Ile Ile Tyr Ser Pro Tyr Gln 
                165                 170                 175     


Ser Met Glu Ala Thr Pro Tyr Lys Asp Gly Phe Gly Ser Ile Met Thr 
            180                 185                 190         


Val Glu Phe Ser Pro Glu Tyr Ala Thr Ala Phe Asn Asp Ile Ser Ile 
        195                 200                 205             


Ala Ser His Ser Pro Ser Leu Phe Ile Lys Asp Pro Ala Leu Ile Leu 
    210                 215                 220                 


Met His Glu Leu Ile His Val Leu His Gly Leu Tyr Gly Thr Tyr Ile 
225                 230                 235                 240 


Thr Glu Tyr Lys Ile Thr Pro Asn Val Val Gln Ser Tyr Met Lys Val 
                245                 250                 255     


Thr Lys Pro Ile Thr Ser Ala Glu Phe Leu Thr Phe Gly Gly Arg Asp 
            260                 265                 270         


Arg Asn Ile Val Pro Gln Ser Ile Gln Ser Gln Leu Tyr Asn Lys Val 
        275                 280                 285             


Leu Ser Asp Tyr Lys Arg Ile Ala Ser Arg Leu Asn Lys Val Asn Thr 
    290                 295                 300                 


Ala Thr Ala Leu Ile Asn Ile Asp Glu Phe Lys Asn Leu Tyr Glu Trp 
305                 310                 315                 320 


Lys Tyr Gln Phe Ala Lys Asp Ser Asn Gly Val Tyr Ser Val Asp Leu 
                325                 330                 335     


Asn Lys Phe Glu Gln Leu Tyr Lys Lys Ile Tyr Ser Phe Thr Glu Phe 
            340                 345                 350         


Asn Leu Ala Tyr Glu Phe Lys Ile Lys Thr Arg Leu Gly Tyr Leu Ala 
        355                 360                 365             


Glu Asn Phe Gly Pro Phe Tyr Leu Pro Asn Leu Leu Asp Asp Ser Ile 
    370                 375                 380                 


Tyr Thr Glu Val Asp Gly Phe Asn Ile Gly Ala Leu Ser Ile Asn Tyr 
385                 390                 395                 400 


Gln Gly Gln Asn Ile Gly Ser Asp Ile Asn Ser Ile Lys Lys Leu Gln 
                405                 410                 415     


Gly Gln Gly Val Val Ser Arg Val Val Arg Leu Cys Ser Asn Ser Glu 
            420                 425                 430         


Asn Leu Tyr Phe Gln Gly Ala Ser His His His His His His His His 
        435                 440                 445             


<210>  33
<211>  3888
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide Sequence of rBoNT/F(0) (His-tagged)

<400>  33
atgccggttg tgattaacag cttcaattat aacgatccgg tgaacgatga taccatcctg       60

tatatgcaga ttccgtatga agagaaaagc aaaaagtact acaaagcctt tgagatcatg      120

cgcaacgttt ggattattcc ggaacgtaat accattggca ccgatccgag cgattttgat      180

ccgcctgcaa gcctggaaaa tggtagcagc gcatattatg atccgaatta tctgaccacc      240

gatgccgaaa aagatcgtta tctgaaaacc accatcaaac tgttcaaacg cattaatagc      300

aatccggcag gcgaagttct gctgcaagaa attagctatg caaaaccgta tctgggcaat      360

gaacataccc cgattaatga atttcatccg gttacacgta ccacgagcgt taacattaaa      420

agcagcacca atgtgaagtc cagcattatt ctgaatctgc tggttttagg tgcaggtccg      480

gatatttttg aaaattcaag ctatccggtg cgcaaactga tggatagcgg tggtgtgtat      540

gatccgtcaa atgatggttt tggcagcatt aacattgtga cctttagtcc ggaatatgaa      600

tacaccttca acgatattag cggtggctat aatagcagca ccgaaagttt tattgcagat      660

ccggcaatta gcctggcaca ccagctgatt tatgcactgc atggtctgta tggtgcacgt      720

ggtgttacct ataaagaaac cattaaagtt aaacaggcac cgctgatgat tgcggaaaaa      780

ccgattcgtc tggaagaatt tctgaccttt ggtggtcagg atctgaacat tattaccagc      840

gcaatgaaag agaaaatcta taataacctg ctggccaact atgagaaaat tgcaacccgt      900

ctgagccgtg ttaatagcgc acctcctgaa tatgatatca acgagtataa agactatttt      960

cagtggaaat acggcctgga taaaaatgca gatggtagct ataccgtgaa cgagaacaaa     1020

tttaacgaga tctacaaaaa actgtatagc ttcaccgaaa tcgatctggc caacaaattc     1080

aaagtgaaat gccgcaacac ctacttcatc aaatatggct ttctgaaagt tccgaacctg     1140

cttgatgatg atatctatac cgttagcgaa ggctttaaca ttggtaatct ggccgttaat     1200

aatcgcggtc agaacattaa actgaacccg aaaattatcg atagcatccc ggataaaggc     1260

ctggttgaaa aaattgtgaa attctgcaaa agcgtgattc cgcgtaaagg caccaaagca     1320

ccgcctcgtc tgtgtattcg tgtgaataat cgtgaactgt tttttgttgc aagcgagagc     1380

agctataacg agaatgatat taacaccccg aaagagattg acgataccac caatctgaat     1440

aacaactatc gcaacaatct ggatgaagtg atcctggatt ataacagcga aaccattccg     1500

cagattagca atcagaccct gaataccctg gttcaggatg atagctatgt tccgcgttat     1560

gatagcaatg gcaccagcga aattgaagaa cataatgtgg ttgatctgaa cgtgttcttt     1620

tatctgcatg cacagaaagt gccggaaggt gaaaccaata ttagcctgac cagcagcatt     1680

gataccgcac tgagcgaaga aagccaggtt tatacctttt ttagcagcga attcatcaac     1740

accattaaca aaccggttca tgcagcactg tttattagct ggattaatca ggtgattcgc     1800

gattttacca ccgaagcaac ccagaaaagc acctttgata aaattgccga tattagtctg     1860

gtggtgccgt atgttggtct ggcactgaat attggtaatg aagtgcagaa agagaacttt     1920

aaagaagcct tcgaactgtt aggtgccggt attctgctgg aatttgtgcc ggaactgctg     1980

attccgacca ttctggtttt taccattaag agctttattg gcagcagcga gaacaagaac     2040

aaaatcatta aagccatcaa caacagcctg atggaacgcg aaaccaaatg gaaagaaatt     2100

tacagctgga ttgtgagcaa ttggctgacc cgtatcaata cccagtttaa caaacgcaaa     2160

gaacaaatgt atcaggccct gcagaatcag gttgatgcaa ttaaaaccgt gatcgaatac     2220

aaatacaaca actataccag cgacgaacgt aatcgcctgg aaagcgaata caacattaat     2280

aacattcgcg aagaactgaa caaaaaagtg agcctggcaa tggaaaacat cgaacgtttt     2340

attaccgaaa gcagcatctt ctacctgatg aaactgatta acgaagccaa agttagcaaa     2400

ctgcgcgaat atgatgaagg cgttaaagaa tatctgctgg actatattag cgaacatcgt     2460

agcattctgg gtaatagcgt tcaagagctg aatgatctgg ttaccagcac actgaataat     2520

agcattccgt ttgaactgag cagctacacc aacgataaaa tcctgatcct gtacttcaac     2580

aaactgtaca agaagatcaa ggacaacagc atactggata tgcgctatga aaacaacaag     2640

ttcattgata tcagcggcta tggtagcaac attagcatta atggtgatgt gtatatctac     2700

agcaccaacc gcaatcagtt tggtatttat agcagcaaac cgagcgaagt taatattgcg     2760

cagaataacg atatcatcta caacggtcgc tatcagaact ttagcattag cttttgggtt     2820

cgcattccga aatactttaa caaggtgaac ctgaacaacg agtacaccat tattgattgc     2880

attcgcaata ataacagcgg ctggaaaatc agcctgaact ataacaaaat tatctggacc     2940

ctgcaggata ccgcaggtaa taatcagaaa ctggtgttta actacaccca gatgattagc     3000

atcagcgact atatcaacaa atggatcttt gtgaccatta ccaacaatcg tctgggtaac     3060

agccgcattt atatcaatgg caatctgatc gacgaaaaaa gcatttcaaa tctgggcgat     3120

attcacgtga gcgataacat tctgttcaaa attgttggct gcaacgatac ccgttatgtt     3180

ggtattcgtt acttcaaagt gtttgatacg gaactgggca aaacggaaat tgaaaccctg     3240

tatagtgatg aaccggatcc gagcattctg aaagattttt ggggtaatta tctgctgtac     3300

aacaaacgct actatctgct gaacctgctg cgtaccgata aaagcattac acagaatagc     3360

aactttctga acatcaatca gcagcgtggt gtttatcaga aaccgaacat ttttagcaac     3420

acccgtctgt ataccggtgt ggaagttatt attcgtaaaa acggtagcac cgatatcagc     3480

aacaccgata actttgtgcg taaaaatgac ctggcctata ttaacgttgt tgatcgtgat     3540

gttgagtatc gtctgtatgc ggatattagc attgccaaac cggaaaagat tatcaaactg     3600

atccgtacca gcaacagcaa taattcactg ggtcagatta tcgtgatgga cagcattggt     3660

aacaattgca ccatgaattt ccagaacaat aacggtggta atattggcct gctgggcttt     3720

catagcaata atctggttgc aagcagctgg tattacaaca acatccgtaa aaataccagc     3780

agtaatggtt gcttttggag ctttatcagt aaagaacatg gctggcaaga aaacgagaac     3840

ctgtattttc agggtgcaag tcatcatcac catcaccacc atcattaa                  3888


<210>  34
<211>  1295
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide Sequence of rBoNT/F(0) (His-tagged)

<400>  34

Met Pro Val Val Ile Asn Ser Phe Asn Tyr Asn Asp Pro Val Asn Asp 
1               5                   10                  15      


Asp Thr Ile Leu Tyr Met Gln Ile Pro Tyr Glu Glu Lys Ser Lys Lys 
            20                  25                  30          


Tyr Tyr Lys Ala Phe Glu Ile Met Arg Asn Val Trp Ile Ile Pro Glu 
        35                  40                  45              


Arg Asn Thr Ile Gly Thr Asp Pro Ser Asp Phe Asp Pro Pro Ala Ser 
    50                  55                  60                  


Leu Glu Asn Gly Ser Ser Ala Tyr Tyr Asp Pro Asn Tyr Leu Thr Thr 
65                  70                  75                  80  


Asp Ala Glu Lys Asp Arg Tyr Leu Lys Thr Thr Ile Lys Leu Phe Lys 
                85                  90                  95      


Arg Ile Asn Ser Asn Pro Ala Gly Glu Val Leu Leu Gln Glu Ile Ser 
            100                 105                 110         


Tyr Ala Lys Pro Tyr Leu Gly Asn Glu His Thr Pro Ile Asn Glu Phe 
        115                 120                 125             


His Pro Val Thr Arg Thr Thr Ser Val Asn Ile Lys Ser Ser Thr Asn 
    130                 135                 140                 


Val Lys Ser Ser Ile Ile Leu Asn Leu Leu Val Leu Gly Ala Gly Pro 
145                 150                 155                 160 


Asp Ile Phe Glu Asn Ser Ser Tyr Pro Val Arg Lys Leu Met Asp Ser 
                165                 170                 175     


Gly Gly Val Tyr Asp Pro Ser Asn Asp Gly Phe Gly Ser Ile Asn Ile 
            180                 185                 190         


Val Thr Phe Ser Pro Glu Tyr Glu Tyr Thr Phe Asn Asp Ile Ser Gly 
        195                 200                 205             


Gly Tyr Asn Ser Ser Thr Glu Ser Phe Ile Ala Asp Pro Ala Ile Ser 
    210                 215                 220                 


Leu Ala His Gln Leu Ile Tyr Ala Leu His Gly Leu Tyr Gly Ala Arg 
225                 230                 235                 240 


Gly Val Thr Tyr Lys Glu Thr Ile Lys Val Lys Gln Ala Pro Leu Met 
                245                 250                 255     


Ile Ala Glu Lys Pro Ile Arg Leu Glu Glu Phe Leu Thr Phe Gly Gly 
            260                 265                 270         


Gln Asp Leu Asn Ile Ile Thr Ser Ala Met Lys Glu Lys Ile Tyr Asn 
        275                 280                 285             


Asn Leu Leu Ala Asn Tyr Glu Lys Ile Ala Thr Arg Leu Ser Arg Val 
    290                 295                 300                 


Asn Ser Ala Pro Pro Glu Tyr Asp Ile Asn Glu Tyr Lys Asp Tyr Phe 
305                 310                 315                 320 


Gln Trp Lys Tyr Gly Leu Asp Lys Asn Ala Asp Gly Ser Tyr Thr Val 
                325                 330                 335     


Asn Glu Asn Lys Phe Asn Glu Ile Tyr Lys Lys Leu Tyr Ser Phe Thr 
            340                 345                 350         


Glu Ile Asp Leu Ala Asn Lys Phe Lys Val Lys Cys Arg Asn Thr Tyr 
        355                 360                 365             


Phe Ile Lys Tyr Gly Phe Leu Lys Val Pro Asn Leu Leu Asp Asp Asp 
    370                 375                 380                 


Ile Tyr Thr Val Ser Glu Gly Phe Asn Ile Gly Asn Leu Ala Val Asn 
385                 390                 395                 400 


Asn Arg Gly Gln Asn Ile Lys Leu Asn Pro Lys Ile Ile Asp Ser Ile 
                405                 410                 415     


Pro Asp Lys Gly Leu Val Glu Lys Ile Val Lys Phe Cys Lys Ser Val 
            420                 425                 430         


Ile Pro Arg Lys Gly Thr Lys Ala Pro Pro Arg Leu Cys Ile Arg Val 
        435                 440                 445             


Asn Asn Arg Glu Leu Phe Phe Val Ala Ser Glu Ser Ser Tyr Asn Glu 
    450                 455                 460                 


Asn Asp Ile Asn Thr Pro Lys Glu Ile Asp Asp Thr Thr Asn Leu Asn 
465                 470                 475                 480 


Asn Asn Tyr Arg Asn Asn Leu Asp Glu Val Ile Leu Asp Tyr Asn Ser 
                485                 490                 495     


Glu Thr Ile Pro Gln Ile Ser Asn Gln Thr Leu Asn Thr Leu Val Gln 
            500                 505                 510         


Asp Asp Ser Tyr Val Pro Arg Tyr Asp Ser Asn Gly Thr Ser Glu Ile 
        515                 520                 525             


Glu Glu His Asn Val Val Asp Leu Asn Val Phe Phe Tyr Leu His Ala 
    530                 535                 540                 


Gln Lys Val Pro Glu Gly Glu Thr Asn Ile Ser Leu Thr Ser Ser Ile 
545                 550                 555                 560 


Asp Thr Ala Leu Ser Glu Glu Ser Gln Val Tyr Thr Phe Phe Ser Ser 
                565                 570                 575     


Glu Phe Ile Asn Thr Ile Asn Lys Pro Val His Ala Ala Leu Phe Ile 
            580                 585                 590         


Ser Trp Ile Asn Gln Val Ile Arg Asp Phe Thr Thr Glu Ala Thr Gln 
        595                 600                 605             


Lys Ser Thr Phe Asp Lys Ile Ala Asp Ile Ser Leu Val Val Pro Tyr 
    610                 615                 620                 


Val Gly Leu Ala Leu Asn Ile Gly Asn Glu Val Gln Lys Glu Asn Phe 
625                 630                 635                 640 


Lys Glu Ala Phe Glu Leu Leu Gly Ala Gly Ile Leu Leu Glu Phe Val 
                645                 650                 655     


Pro Glu Leu Leu Ile Pro Thr Ile Leu Val Phe Thr Ile Lys Ser Phe 
            660                 665                 670         


Ile Gly Ser Ser Glu Asn Lys Asn Lys Ile Ile Lys Ala Ile Asn Asn 
        675                 680                 685             


Ser Leu Met Glu Arg Glu Thr Lys Trp Lys Glu Ile Tyr Ser Trp Ile 
    690                 695                 700                 


Val Ser Asn Trp Leu Thr Arg Ile Asn Thr Gln Phe Asn Lys Arg Lys 
705                 710                 715                 720 


Glu Gln Met Tyr Gln Ala Leu Gln Asn Gln Val Asp Ala Ile Lys Thr 
                725                 730                 735     


Val Ile Glu Tyr Lys Tyr Asn Asn Tyr Thr Ser Asp Glu Arg Asn Arg 
            740                 745                 750         


Leu Glu Ser Glu Tyr Asn Ile Asn Asn Ile Arg Glu Glu Leu Asn Lys 
        755                 760                 765             


Lys Val Ser Leu Ala Met Glu Asn Ile Glu Arg Phe Ile Thr Glu Ser 
    770                 775                 780                 


Ser Ile Phe Tyr Leu Met Lys Leu Ile Asn Glu Ala Lys Val Ser Lys 
785                 790                 795                 800 


Leu Arg Glu Tyr Asp Glu Gly Val Lys Glu Tyr Leu Leu Asp Tyr Ile 
                805                 810                 815     


Ser Glu His Arg Ser Ile Leu Gly Asn Ser Val Gln Glu Leu Asn Asp 
            820                 825                 830         


Leu Val Thr Ser Thr Leu Asn Asn Ser Ile Pro Phe Glu Leu Ser Ser 
        835                 840                 845             


Tyr Thr Asn Asp Lys Ile Leu Ile Leu Tyr Phe Asn Lys Leu Tyr Lys 
    850                 855                 860                 


Lys Ile Lys Asp Asn Ser Ile Leu Asp Met Arg Tyr Glu Asn Asn Lys 
865                 870                 875                 880 


Phe Ile Asp Ile Ser Gly Tyr Gly Ser Asn Ile Ser Ile Asn Gly Asp 
                885                 890                 895     


Val Tyr Ile Tyr Ser Thr Asn Arg Asn Gln Phe Gly Ile Tyr Ser Ser 
            900                 905                 910         


Lys Pro Ser Glu Val Asn Ile Ala Gln Asn Asn Asp Ile Ile Tyr Asn 
        915                 920                 925             


Gly Arg Tyr Gln Asn Phe Ser Ile Ser Phe Trp Val Arg Ile Pro Lys 
    930                 935                 940                 


Tyr Phe Asn Lys Val Asn Leu Asn Asn Glu Tyr Thr Ile Ile Asp Cys 
945                 950                 955                 960 


Ile Arg Asn Asn Asn Ser Gly Trp Lys Ile Ser Leu Asn Tyr Asn Lys 
                965                 970                 975     


Ile Ile Trp Thr Leu Gln Asp Thr Ala Gly Asn Asn Gln Lys Leu Val 
            980                 985                 990         


Phe Asn Tyr Thr Gln Met Ile Ser  Ile Ser Asp Tyr Ile  Asn Lys Trp 
        995                 1000                 1005             


Ile Phe  Val Thr Ile Thr Asn  Asn Arg Leu Gly Asn  Ser Arg Ile 
    1010                 1015                 1020             


Tyr Ile  Asn Gly Asn Leu Ile  Asp Glu Lys Ser Ile  Ser Asn Leu 
    1025                 1030                 1035             


Gly Asp  Ile His Val Ser Asp  Asn Ile Leu Phe Lys  Ile Val Gly 
    1040                 1045                 1050             


Cys Asn  Asp Thr Arg Tyr Val  Gly Ile Arg Tyr Phe  Lys Val Phe 
    1055                 1060                 1065             


Asp Thr  Glu Leu Gly Lys Thr  Glu Ile Glu Thr Leu  Tyr Ser Asp 
    1070                 1075                 1080             


Glu Pro  Asp Pro Ser Ile Leu  Lys Asp Phe Trp Gly  Asn Tyr Leu 
    1085                 1090                 1095             


Leu Tyr  Asn Lys Arg Tyr Tyr  Leu Leu Asn Leu Leu  Arg Thr Asp 
    1100                 1105                 1110             


Lys Ser  Ile Thr Gln Asn Ser  Asn Phe Leu Asn Ile  Asn Gln Gln 
    1115                 1120                 1125             


Arg Gly  Val Tyr Gln Lys Pro  Asn Ile Phe Ser Asn  Thr Arg Leu 
    1130                 1135                 1140             


Tyr Thr  Gly Val Glu Val Ile  Ile Arg Lys Asn Gly  Ser Thr Asp 
    1145                 1150                 1155             


Ile Ser  Asn Thr Asp Asn Phe  Val Arg Lys Asn Asp  Leu Ala Tyr 
    1160                 1165                 1170             


Ile Asn  Val Val Asp Arg Asp  Val Glu Tyr Arg Leu  Tyr Ala Asp 
    1175                 1180                 1185             


Ile Ser  Ile Ala Lys Pro Glu  Lys Ile Ile Lys Leu  Ile Arg Thr 
    1190                 1195                 1200             


Ser Asn  Ser Asn Asn Ser Leu  Gly Gln Ile Ile Val  Met Asp Ser 
    1205                 1210                 1215             


Ile Gly  Asn Asn Cys Thr Met  Asn Phe Gln Asn Asn  Asn Gly Gly 
    1220                 1225                 1230             


Asn Ile  Gly Leu Leu Gly Phe  His Ser Asn Asn Leu  Val Ala Ser 
    1235                 1240                 1245             


Ser Trp  Tyr Tyr Asn Asn Ile  Arg Lys Asn Thr Ser  Ser Asn Gly 
    1250                 1255                 1260             


Cys Phe  Trp Ser Phe Ile Ser  Lys Glu His Gly Trp  Gln Glu Asn 
    1265                 1270                 1275             


Glu Asn  Leu Tyr Phe Gln Gly  Ala Ser His His His  His His His 
    1280                 1285                 1290             


His His  
    1295 


<210>  35
<211>  2649
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide Sequence of rLHN/F (His-tagged)

<400>  35
atgccggttg tgattaacag cttcaattat aacgatccgg tgaacgatga taccatcctg       60

tatatgcaga ttccgtatga agagaaaagc aaaaagtact acaaagcctt tgagatcatg      120

cgcaacgttt ggattattcc ggaacgtaat accattggca ccgatccgag cgattttgat      180

ccgcctgcaa gcctggaaaa tggtagcagc gcatattatg atccgaatta tctgaccacc      240

gatgccgaaa aagatcgtta tctgaaaacc accatcaaac tgttcaaacg cattaatagc      300

aatccggcag gcgaagttct gctgcaagaa attagctatg caaaaccgta tctgggcaat      360

gaacataccc cgattaatga atttcatccg gttacacgta ccacgagcgt taacattaaa      420

agcagcacca atgtgaagtc cagcattatt ctgaatctgc tggttttagg tgcaggtccg      480

gatatttttg aaaattcaag ctatccggtg cgcaaactga tggatagcgg tggtgtgtat      540

gatccgtcaa atgatggttt tggcagcatt aacattgtga cctttagtcc ggaatatgaa      600

tacaccttca acgatattag cggtggctat aatagcagca ccgaaagttt tattgcagat      660

ccggcaatta gcctggcaca tgaactgatt catgcactgc atggtctgta tggtgcacgt      720

ggtgttacct ataaagaaac cattaaagtt aaacaggcac cgctgatgat tgcggaaaaa      780

ccgattcgtc tggaagaatt tctgaccttt ggtggtcagg atctgaacat tattaccagc      840

gcaatgaaag agaaaatcta taataacctg ctggccaact atgagaaaat tgcaacccgt      900

ctgagccgtg ttaatagcgc acctcctgaa tatgatatca acgagtataa agactatttt      960

cagtggaaat acggcctgga taaaaatgca gatggtagct ataccgtgaa cgagaacaaa     1020

tttaacgaga tctacaaaaa actgtatagc ttcaccgaaa tcgatctggc caacaaattc     1080

aaagtgaaat gccgcaacac ctacttcatc aaatatggct ttctgaaagt tccgaacctg     1140

cttgatgatg atatctatac cgttagcgaa ggctttaaca ttggtaatct ggccgttaat     1200

aatcgcggtc agaacattaa actgaacccg aaaattatcg atagcatccc ggataaaggc     1260

ctggttgaaa aaattgtgaa attctgcaaa agcgtgattc cgcgtaaagg caccaaagca     1320

ccgcctcgtc tgtgtattcg tgtgaataat cgtgaactgt tttttgttgc aagcgagagc     1380

agctataacg agaatgatat taacaccccg aaagagattg acgataccac caatctgaat     1440

aacaactatc gcaacaatct ggatgaagtg atcctggatt ataacagcga aaccattccg     1500

cagattagca atcagaccct gaataccctg gttcaggatg atagctatgt tccgcgttat     1560

gatagcaatg gcaccagcga aattgaagaa cataatgtgg ttgatctgaa cgtgttcttt     1620

tatctgcatg cacagaaagt gccggaaggt gaaaccaata ttagcctgac cagcagcatt     1680

gataccgcac tgagcgaaga aagccaggtt tatacctttt ttagcagcga attcatcaac     1740

accattaaca aaccggttca tgcagcactg tttattagct ggattaatca ggtgattcgc     1800

gattttacca ccgaagcaac ccagaaaagc acctttgata aaattgccga tattagtctg     1860

gtggtgccgt atgttggtct ggcactgaat attggtaatg aagtgcagaa agagaacttt     1920

aaagaagcct tcgaactgtt aggtgccggt attctgctgg aatttgtgcc ggaactgctg     1980

attccgacca ttctggtttt taccattaag agctttattg gcagcagcga gaacaagaac     2040

aaaatcatta aagccatcaa caacagcctg atggaacgcg aaaccaaatg gaaagaaatt     2100

tacagctgga ttgtgagcaa ttggctgacc cgtatcaata cccagtttaa caaacgcaaa     2160

gaacaaatgt atcaggccct gcagaatcag gttgatgcaa ttaaaaccgt gatcgaatac     2220

aaatacaaca actataccag cgacgaacgt aatcgcctgg aaagcgaata caacattaat     2280

aacattcgcg aagaactgaa caaaaaagtg agcctggcaa tggaaaacat cgaacgtttt     2340

attaccgaaa gcagcatctt ctacctgatg aaactgatta acgaagccaa agttagcaaa     2400

ctgcgcgaat atgatgaagg cgttaaagaa tatctgctgg actatattag cgaacatcgt     2460

agcattctgg gtaatagcgt tcaagagctg aatgatctgg ttaccagcac actgaataat     2520

agcattccgt ttgaactgag cagctacacc aacgataaaa tcctgatcct gtacttcaac     2580

aaactgtaca agaaagaaaa cctgtatttt cagggtgcaa gccatcatca ccaccatcac     2640

catcattaa                                                             2649


<210>  36
<211>  882
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide Sequence of rLHN/F (His-tagged)

<400>  36

Met Pro Val Val Ile Asn Ser Phe Asn Tyr Asn Asp Pro Val Asn Asp 
1               5                   10                  15      


Asp Thr Ile Leu Tyr Met Gln Ile Pro Tyr Glu Glu Lys Ser Lys Lys 
            20                  25                  30          


Tyr Tyr Lys Ala Phe Glu Ile Met Arg Asn Val Trp Ile Ile Pro Glu 
        35                  40                  45              


Arg Asn Thr Ile Gly Thr Asp Pro Ser Asp Phe Asp Pro Pro Ala Ser 
    50                  55                  60                  


Leu Glu Asn Gly Ser Ser Ala Tyr Tyr Asp Pro Asn Tyr Leu Thr Thr 
65                  70                  75                  80  


Asp Ala Glu Lys Asp Arg Tyr Leu Lys Thr Thr Ile Lys Leu Phe Lys 
                85                  90                  95      


Arg Ile Asn Ser Asn Pro Ala Gly Glu Val Leu Leu Gln Glu Ile Ser 
            100                 105                 110         


Tyr Ala Lys Pro Tyr Leu Gly Asn Glu His Thr Pro Ile Asn Glu Phe 
        115                 120                 125             


His Pro Val Thr Arg Thr Thr Ser Val Asn Ile Lys Ser Ser Thr Asn 
    130                 135                 140                 


Val Lys Ser Ser Ile Ile Leu Asn Leu Leu Val Leu Gly Ala Gly Pro 
145                 150                 155                 160 


Asp Ile Phe Glu Asn Ser Ser Tyr Pro Val Arg Lys Leu Met Asp Ser 
                165                 170                 175     


Gly Gly Val Tyr Asp Pro Ser Asn Asp Gly Phe Gly Ser Ile Asn Ile 
            180                 185                 190         


Val Thr Phe Ser Pro Glu Tyr Glu Tyr Thr Phe Asn Asp Ile Ser Gly 
        195                 200                 205             


Gly Tyr Asn Ser Ser Thr Glu Ser Phe Ile Ala Asp Pro Ala Ile Ser 
    210                 215                 220                 


Leu Ala His Glu Leu Ile His Ala Leu His Gly Leu Tyr Gly Ala Arg 
225                 230                 235                 240 


Gly Val Thr Tyr Lys Glu Thr Ile Lys Val Lys Gln Ala Pro Leu Met 
                245                 250                 255     


Ile Ala Glu Lys Pro Ile Arg Leu Glu Glu Phe Leu Thr Phe Gly Gly 
            260                 265                 270         


Gln Asp Leu Asn Ile Ile Thr Ser Ala Met Lys Glu Lys Ile Tyr Asn 
        275                 280                 285             


Asn Leu Leu Ala Asn Tyr Glu Lys Ile Ala Thr Arg Leu Ser Arg Val 
    290                 295                 300                 


Asn Ser Ala Pro Pro Glu Tyr Asp Ile Asn Glu Tyr Lys Asp Tyr Phe 
305                 310                 315                 320 


Gln Trp Lys Tyr Gly Leu Asp Lys Asn Ala Asp Gly Ser Tyr Thr Val 
                325                 330                 335     


Asn Glu Asn Lys Phe Asn Glu Ile Tyr Lys Lys Leu Tyr Ser Phe Thr 
            340                 345                 350         


Glu Ile Asp Leu Ala Asn Lys Phe Lys Val Lys Cys Arg Asn Thr Tyr 
        355                 360                 365             


Phe Ile Lys Tyr Gly Phe Leu Lys Val Pro Asn Leu Leu Asp Asp Asp 
    370                 375                 380                 


Ile Tyr Thr Val Ser Glu Gly Phe Asn Ile Gly Asn Leu Ala Val Asn 
385                 390                 395                 400 


Asn Arg Gly Gln Asn Ile Lys Leu Asn Pro Lys Ile Ile Asp Ser Ile 
                405                 410                 415     


Pro Asp Lys Gly Leu Val Glu Lys Ile Val Lys Phe Cys Lys Ser Val 
            420                 425                 430         


Ile Pro Arg Lys Gly Thr Lys Ala Pro Pro Arg Leu Cys Ile Arg Val 
        435                 440                 445             


Asn Asn Arg Glu Leu Phe Phe Val Ala Ser Glu Ser Ser Tyr Asn Glu 
    450                 455                 460                 


Asn Asp Ile Asn Thr Pro Lys Glu Ile Asp Asp Thr Thr Asn Leu Asn 
465                 470                 475                 480 


Asn Asn Tyr Arg Asn Asn Leu Asp Glu Val Ile Leu Asp Tyr Asn Ser 
                485                 490                 495     


Glu Thr Ile Pro Gln Ile Ser Asn Gln Thr Leu Asn Thr Leu Val Gln 
            500                 505                 510         


Asp Asp Ser Tyr Val Pro Arg Tyr Asp Ser Asn Gly Thr Ser Glu Ile 
        515                 520                 525             


Glu Glu His Asn Val Val Asp Leu Asn Val Phe Phe Tyr Leu His Ala 
    530                 535                 540                 


Gln Lys Val Pro Glu Gly Glu Thr Asn Ile Ser Leu Thr Ser Ser Ile 
545                 550                 555                 560 


Asp Thr Ala Leu Ser Glu Glu Ser Gln Val Tyr Thr Phe Phe Ser Ser 
                565                 570                 575     


Glu Phe Ile Asn Thr Ile Asn Lys Pro Val His Ala Ala Leu Phe Ile 
            580                 585                 590         


Ser Trp Ile Asn Gln Val Ile Arg Asp Phe Thr Thr Glu Ala Thr Gln 
        595                 600                 605             


Lys Ser Thr Phe Asp Lys Ile Ala Asp Ile Ser Leu Val Val Pro Tyr 
    610                 615                 620                 


Val Gly Leu Ala Leu Asn Ile Gly Asn Glu Val Gln Lys Glu Asn Phe 
625                 630                 635                 640 


Lys Glu Ala Phe Glu Leu Leu Gly Ala Gly Ile Leu Leu Glu Phe Val 
                645                 650                 655     


Pro Glu Leu Leu Ile Pro Thr Ile Leu Val Phe Thr Ile Lys Ser Phe 
            660                 665                 670         


Ile Gly Ser Ser Glu Asn Lys Asn Lys Ile Ile Lys Ala Ile Asn Asn 
        675                 680                 685             


Ser Leu Met Glu Arg Glu Thr Lys Trp Lys Glu Ile Tyr Ser Trp Ile 
    690                 695                 700                 


Val Ser Asn Trp Leu Thr Arg Ile Asn Thr Gln Phe Asn Lys Arg Lys 
705                 710                 715                 720 


Glu Gln Met Tyr Gln Ala Leu Gln Asn Gln Val Asp Ala Ile Lys Thr 
                725                 730                 735     


Val Ile Glu Tyr Lys Tyr Asn Asn Tyr Thr Ser Asp Glu Arg Asn Arg 
            740                 745                 750         


Leu Glu Ser Glu Tyr Asn Ile Asn Asn Ile Arg Glu Glu Leu Asn Lys 
        755                 760                 765             


Lys Val Ser Leu Ala Met Glu Asn Ile Glu Arg Phe Ile Thr Glu Ser 
    770                 775                 780                 


Ser Ile Phe Tyr Leu Met Lys Leu Ile Asn Glu Ala Lys Val Ser Lys 
785                 790                 795                 800 


Leu Arg Glu Tyr Asp Glu Gly Val Lys Glu Tyr Leu Leu Asp Tyr Ile 
                805                 810                 815     


Ser Glu His Arg Ser Ile Leu Gly Asn Ser Val Gln Glu Leu Asn Asp 
            820                 825                 830         


Leu Val Thr Ser Thr Leu Asn Asn Ser Ile Pro Phe Glu Leu Ser Ser 
        835                 840                 845             


Tyr Thr Asn Asp Lys Ile Leu Ile Leu Tyr Phe Asn Lys Leu Tyr Lys 
    850                 855                 860                 


Lys Glu Asn Leu Tyr Phe Gln Gly Ala Ser His His His His His His 
865                 870                 875                 880 


His His 
        


<210>  37
<211>  1296
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide Sequence of rHC/F (His-tagged)

<400>  37
atgatcaagg ataacagcat tctggatatg cgctatgaga acaacaaatt cattgatatt       60

agcggctatg gcagcaacat tagcattaat ggtgatgtgt atatctacag caccaaccgt      120

aatcagtttg gcatttatag cagcaaaccg agcgaagtta atattgccca gaacaacgat      180

atcatctata acggtcgcta tcagaacttc agcattagct tttgggttcg cattccgaaa      240

tacttcaata aggtgaacct gaacaacgag tataccatca ttgattgcat tcgcaataat      300

aacagcggct ggaaaattag cctgaactac aacaaaatta tctggaccct gcaggatacc      360

gcaggtaata atcagaaact ggtgtttaac tacacccaga tgattagcat cagcgactat      420

atcaacaaat ggatctttgt gaccattacc aataatcgcc tgggtaatag ccgcatttat      480

atcaatggta acctgatcga tgagaaaagc attagcaatc tgggtgatat tcatgtgagc      540

gataacatcc tgtttaaaat cgtgggttgt aacgataccc gttatgttgg tattcgctac      600

ttcaaagtgt ttgataccga actgggtaaa accgaaattg aaaccctgta tagtgatgaa      660

ccggatccga gcattctgaa agatttttgg ggtaattatc tgctgtacaa caaacgctac      720

tatctgctga atctgctgcg taccgataaa tcaattaccc agaatagcaa cttcctgaac      780

attaatcagc agcgtggtgt ttatcagaaa ccgaacattt ttagcaacac ccgtctgtat      840

accggtgtgg aagttattat tcgtaaaaat ggcagcaccg atatcagcaa caccgataac      900

tttgttcgca aaaatgatct ggcgtatatc aacgttgttg atcgtgatgt tgaatatcgt      960

ctgtatgccg atattagcat tgccaaaccg gaaaaaatca tcaaactgat ccgtaccagc     1020

aacagcaata attcactggg tcagattatt gtgatggata gcattggtaa taactgcacc     1080

atgaactttc agaacaataa cggtggtaat attggtctgc tgggctttca tagtaataat     1140

ctggttgcaa gcagctggta ttataacaac atccgtaaaa ataccagcag caatggttgc     1200

ttttggagct ttattagcaa agaacatggc tggcaagaaa acgagaatct gtattttcag     1260

ggtgcaagtc atcatcacca ccatcaccat cattaa                               1296


<210>  38
<211>  431
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide Sequence of rHC/F (His-tagged)

<400>  38

Met Ile Lys Asp Asn Ser Ile Leu Asp Met Arg Tyr Glu Asn Asn Lys 
1               5                   10                  15      


Phe Ile Asp Ile Ser Gly Tyr Gly Ser Asn Ile Ser Ile Asn Gly Asp 
            20                  25                  30          


Val Tyr Ile Tyr Ser Thr Asn Arg Asn Gln Phe Gly Ile Tyr Ser Ser 
        35                  40                  45              


Lys Pro Ser Glu Val Asn Ile Ala Gln Asn Asn Asp Ile Ile Tyr Asn 
    50                  55                  60                  


Gly Arg Tyr Gln Asn Phe Ser Ile Ser Phe Trp Val Arg Ile Pro Lys 
65                  70                  75                  80  


Tyr Phe Asn Lys Val Asn Leu Asn Asn Glu Tyr Thr Ile Ile Asp Cys 
                85                  90                  95      


Ile Arg Asn Asn Asn Ser Gly Trp Lys Ile Ser Leu Asn Tyr Asn Lys 
            100                 105                 110         


Ile Ile Trp Thr Leu Gln Asp Thr Ala Gly Asn Asn Gln Lys Leu Val 
        115                 120                 125             


Phe Asn Tyr Thr Gln Met Ile Ser Ile Ser Asp Tyr Ile Asn Lys Trp 
    130                 135                 140                 


Ile Phe Val Thr Ile Thr Asn Asn Arg Leu Gly Asn Ser Arg Ile Tyr 
145                 150                 155                 160 


Ile Asn Gly Asn Leu Ile Asp Glu Lys Ser Ile Ser Asn Leu Gly Asp 
                165                 170                 175     


Ile His Val Ser Asp Asn Ile Leu Phe Lys Ile Val Gly Cys Asn Asp 
            180                 185                 190         


Thr Arg Tyr Val Gly Ile Arg Tyr Phe Lys Val Phe Asp Thr Glu Leu 
        195                 200                 205             


Gly Lys Thr Glu Ile Glu Thr Leu Tyr Ser Asp Glu Pro Asp Pro Ser 
    210                 215                 220                 


Ile Leu Lys Asp Phe Trp Gly Asn Tyr Leu Leu Tyr Asn Lys Arg Tyr 
225                 230                 235                 240 


Tyr Leu Leu Asn Leu Leu Arg Thr Asp Lys Ser Ile Thr Gln Asn Ser 
                245                 250                 255     


Asn Phe Leu Asn Ile Asn Gln Gln Arg Gly Val Tyr Gln Lys Pro Asn 
            260                 265                 270         


Ile Phe Ser Asn Thr Arg Leu Tyr Thr Gly Val Glu Val Ile Ile Arg 
        275                 280                 285             


Lys Asn Gly Ser Thr Asp Ile Ser Asn Thr Asp Asn Phe Val Arg Lys 
    290                 295                 300                 


Asn Asp Leu Ala Tyr Ile Asn Val Val Asp Arg Asp Val Glu Tyr Arg 
305                 310                 315                 320 


Leu Tyr Ala Asp Ile Ser Ile Ala Lys Pro Glu Lys Ile Ile Lys Leu 
                325                 330                 335     


Ile Arg Thr Ser Asn Ser Asn Asn Ser Leu Gly Gln Ile Ile Val Met 
            340                 345                 350         


Asp Ser Ile Gly Asn Asn Cys Thr Met Asn Phe Gln Asn Asn Asn Gly 
        355                 360                 365             


Gly Asn Ile Gly Leu Leu Gly Phe His Ser Asn Asn Leu Val Ala Ser 
    370                 375                 380                 


Ser Trp Tyr Tyr Asn Asn Ile Arg Lys Asn Thr Ser Ser Asn Gly Cys 
385                 390                 395                 400 


Phe Trp Ser Phe Ile Ser Lys Glu His Gly Trp Gln Glu Asn Glu Asn 
                405                 410                 415     


Leu Tyr Phe Gln Gly Ala Ser His His His His His His His His 
            420                 425                 430     


<210>  39
<211>  1347
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide Sequence of rLC/F (His-tagged)

<400>  39
atgccggttg tgattaacag cttcaattat aacgatccgg tgaacgatga taccatcctg       60

tatatgcaga ttccgtatga agagaaaagc aaaaagtact acaaagcctt tgagatcatg      120

cgcaacgttt ggattattcc ggaacgtaat accattggca ccgatccgag cgattttgat      180

ccgcctgcaa gcctggaaaa tggtagcagc gcatattatg atccgaatta tctgaccacc      240

gatgccgaaa aagatcgtta tctgaaaacc accatcaaac tgttcaaacg cattaatagc      300

aatccggcag gcgaagttct gctgcaagaa attagctatg caaaaccgta tctgggcaat      360

gaacataccc cgattaatga atttcatccg gttacacgta ccacgagcgt taacattaaa      420

agcagcacca atgtgaagtc cagcattatt ctgaatctgc tggttttagg tgcaggtccg      480

gatatttttg aaaattcaag ctatccggtg cgcaaactga tggatagcgg tggtgtgtat      540

gatccgtcaa atgatggttt tggcagcatt aacattgtga cctttagtcc ggaatatgaa      600

tacaccttca acgatattag cggtggctat aatagcagca ccgaaagttt tattgcagat      660

ccggcaatta gcctggcaca tgaactgatt catgcactgc atggtctgta tggtgcacgt      720

ggtgttacct ataaagaaac cattaaagtt aaacaggcac cgctgatgat tgcggaaaaa      780

ccgattcgtc tggaagaatt tctgaccttt ggtggtcagg atctgaacat tattaccagc      840

gcaatgaaag agaaaatcta taataacctg ctggccaact atgagaaaat tgcaacccgt      900

ctgagccgtg ttaatagcgc acctcctgaa tatgatatca acgagtataa agactatttt      960

cagtggaaat acggcctgga taaaaatgca gatggtagct ataccgtgaa cgagaacaaa     1020

tttaacgaga tctacaaaaa actgtatagc ttcaccgaaa tcgatctggc caacaaattc     1080

aaagtgaaat gccgcaacac ctacttcatc aaatatggct ttctgaaagt tccgaacctg     1140

cttgatgatg atatctatac cgttagcgaa ggctttaaca ttggtaatct ggccgttaat     1200

aatcgcggtc agaacattaa actgaacccg aaaattatcg atagcatccc ggataaaggc     1260

ctggttgaaa aaattgtgaa attctgcaaa agcgagaacc tgtattttca gggtgcaagt     1320

catcatcacc atcaccacca tcattaa                                         1347


<210>  40
<211>  448
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide Sequence of rLC/F (His-tagged)

<400>  40

Met Pro Val Val Ile Asn Ser Phe Asn Tyr Asn Asp Pro Val Asn Asp 
1               5                   10                  15      


Asp Thr Ile Leu Tyr Met Gln Ile Pro Tyr Glu Glu Lys Ser Lys Lys 
            20                  25                  30          


Tyr Tyr Lys Ala Phe Glu Ile Met Arg Asn Val Trp Ile Ile Pro Glu 
        35                  40                  45              


Arg Asn Thr Ile Gly Thr Asp Pro Ser Asp Phe Asp Pro Pro Ala Ser 
    50                  55                  60                  


Leu Glu Asn Gly Ser Ser Ala Tyr Tyr Asp Pro Asn Tyr Leu Thr Thr 
65                  70                  75                  80  


Asp Ala Glu Lys Asp Arg Tyr Leu Lys Thr Thr Ile Lys Leu Phe Lys 
                85                  90                  95      


Arg Ile Asn Ser Asn Pro Ala Gly Glu Val Leu Leu Gln Glu Ile Ser 
            100                 105                 110         


Tyr Ala Lys Pro Tyr Leu Gly Asn Glu His Thr Pro Ile Asn Glu Phe 
        115                 120                 125             


His Pro Val Thr Arg Thr Thr Ser Val Asn Ile Lys Ser Ser Thr Asn 
    130                 135                 140                 


Val Lys Ser Ser Ile Ile Leu Asn Leu Leu Val Leu Gly Ala Gly Pro 
145                 150                 155                 160 


Asp Ile Phe Glu Asn Ser Ser Tyr Pro Val Arg Lys Leu Met Asp Ser 
                165                 170                 175     


Gly Gly Val Tyr Asp Pro Ser Asn Asp Gly Phe Gly Ser Ile Asn Ile 
            180                 185                 190         


Val Thr Phe Ser Pro Glu Tyr Glu Tyr Thr Phe Asn Asp Ile Ser Gly 
        195                 200                 205             


Gly Tyr Asn Ser Ser Thr Glu Ser Phe Ile Ala Asp Pro Ala Ile Ser 
    210                 215                 220                 


Leu Ala His Glu Leu Ile His Ala Leu His Gly Leu Tyr Gly Ala Arg 
225                 230                 235                 240 


Gly Val Thr Tyr Lys Glu Thr Ile Lys Val Lys Gln Ala Pro Leu Met 
                245                 250                 255     


Ile Ala Glu Lys Pro Ile Arg Leu Glu Glu Phe Leu Thr Phe Gly Gly 
            260                 265                 270         


Gln Asp Leu Asn Ile Ile Thr Ser Ala Met Lys Glu Lys Ile Tyr Asn 
        275                 280                 285             


Asn Leu Leu Ala Asn Tyr Glu Lys Ile Ala Thr Arg Leu Ser Arg Val 
    290                 295                 300                 


Asn Ser Ala Pro Pro Glu Tyr Asp Ile Asn Glu Tyr Lys Asp Tyr Phe 
305                 310                 315                 320 


Gln Trp Lys Tyr Gly Leu Asp Lys Asn Ala Asp Gly Ser Tyr Thr Val 
                325                 330                 335     


Asn Glu Asn Lys Phe Asn Glu Ile Tyr Lys Lys Leu Tyr Ser Phe Thr 
            340                 345                 350         


Glu Ile Asp Leu Ala Asn Lys Phe Lys Val Lys Cys Arg Asn Thr Tyr 
        355                 360                 365             


Phe Ile Lys Tyr Gly Phe Leu Lys Val Pro Asn Leu Leu Asp Asp Asp 
    370                 375                 380                 


Ile Tyr Thr Val Ser Glu Gly Phe Asn Ile Gly Asn Leu Ala Val Asn 
385                 390                 395                 400 


Asn Arg Gly Gln Asn Ile Lys Leu Asn Pro Lys Ile Ile Asp Ser Ile 
                405                 410                 415     


Pro Asp Lys Gly Leu Val Glu Lys Ile Val Lys Phe Cys Lys Ser Glu 
            420                 425                 430         


Asn Leu Tyr Phe Gln Gly Ala Ser His His His His His His His His 
        435                 440                 445             


<210>  41
<211>  1317
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide Sequence of Cationic rHC/A (His-tagged)

<400>  41
atgatcatca acaccagcat tctgaacctg cgttatgaaa gcaaacatct gattgatctg       60

agccgttatg ccagcaaaat caatataggc agcaaggtta acttcgaccc gattgacaaa      120

aatcagatac agctgtttaa tctggaaagc agcaaaattg aggtgatcct gaaaaaagcg      180

atcgtgtata atagcatgta cgagaatttt tcgaccagct tttggattcg catcccgaaa      240

tactttaaca agattagcct gaacaacgag tataccatca ttaactgcat ggaaaacaat      300

agcggttgga aagtcagcct gaattatggc gaaattatct ggaccctgca ggataccaaa      360

gaaatcaaac agcgtgtggt gttcaaatac agccagatga ttaatatcag cgactatatc      420

aaccgctgga tttttgtgac cattaccaat aatcggctga acaagagcaa gatctatatt      480

aacggtcgtc tgattgacca gaaaccgatt agtaatctgg gtaatattca tgcgagcaac      540

aaaatcatgt ttaaactgga tggttgccgt gatacccatc gttatatttg gatcaaatac      600

ttcaacctgt tcgataaaga gttgaacgaa aaagaaatta aagacctgta cgataaccag      660

agcaatagcg gcatactgaa agatttttgg ggagattatc tgcagtatga caaaccgtat      720

tatatgctga atctgtacga cccgaataaa tacgtggatg ttaataatgt gggcatccgt      780

ggttatatgt acctgaaagg tccgcgtggt agcgttatga ccacaaacat ttatctgaat      840

agcagcctgt atcgcggaac caaattcatc attaaaaagt atgccagcgg caacaaggat      900

aatattgtgc gtaataatga tcgcgtgtac attaacgttg tggtgaagaa taaagaatat      960

cgcctggcaa ccaatgcaag ccaggcaggc gttgaaaaaa ttctgagtgc cctggaaatt     1020

ccggatgttg gtaatctgag ccaggttgtt gtgatgaaaa gcaaaaacga taaaggcatc     1080

accaacaaat gcaagatgaa tctgcaggac aataacggca atgatattgg cttcattggc     1140

tttcaccagt ttaacaacat tgcaaaactg gttgcgagca attggtataa tcgtcagatt     1200

gaacgtagca gtcgtaccct gggttgtagc tgggaattta tccctgtgga tgatggttgg     1260

ggtgaacgtc cgctgaagct tgcggccgca ctcgagcacc accaccacca ccactga        1317


<210>  42
<211>  438
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide Sequence of Cationic rHC/A (His-tagged)

<400>  42

Met Ile Ile Asn Thr Ser Ile Leu Asn Leu Arg Tyr Glu Ser Lys His 
1               5                   10                  15      


Leu Ile Asp Leu Ser Arg Tyr Ala Ser Lys Ile Asn Ile Gly Ser Lys 
            20                  25                  30          


Val Asn Phe Asp Pro Ile Asp Lys Asn Gln Ile Gln Leu Phe Asn Leu 
        35                  40                  45              


Glu Ser Ser Lys Ile Glu Val Ile Leu Lys Lys Ala Ile Val Tyr Asn 
    50                  55                  60                  


Ser Met Tyr Glu Asn Phe Ser Thr Ser Phe Trp Ile Arg Ile Pro Lys 
65                  70                  75                  80  


Tyr Phe Asn Lys Ile Ser Leu Asn Asn Glu Tyr Thr Ile Ile Asn Cys 
                85                  90                  95      


Met Glu Asn Asn Ser Gly Trp Lys Val Ser Leu Asn Tyr Gly Glu Ile 
            100                 105                 110         


Ile Trp Thr Leu Gln Asp Thr Lys Glu Ile Lys Gln Arg Val Val Phe 
        115                 120                 125             


Lys Tyr Ser Gln Met Ile Asn Ile Ser Asp Tyr Ile Asn Arg Trp Ile 
    130                 135                 140                 


Phe Val Thr Ile Thr Asn Asn Arg Leu Asn Lys Ser Lys Ile Tyr Ile 
145                 150                 155                 160 


Asn Gly Arg Leu Ile Asp Gln Lys Pro Ile Ser Asn Leu Gly Asn Ile 
                165                 170                 175     


His Ala Ser Asn Lys Ile Met Phe Lys Leu Asp Gly Cys Arg Asp Thr 
            180                 185                 190         


His Arg Tyr Ile Trp Ile Lys Tyr Phe Asn Leu Phe Asp Lys Glu Leu 
        195                 200                 205             


Asn Glu Lys Glu Ile Lys Asp Leu Tyr Asp Asn Gln Ser Asn Ser Gly 
    210                 215                 220                 


Ile Leu Lys Asp Phe Trp Gly Asp Tyr Leu Gln Tyr Asp Lys Pro Tyr 
225                 230                 235                 240 


Tyr Met Leu Asn Leu Tyr Asp Pro Asn Lys Tyr Val Asp Val Asn Asn 
                245                 250                 255     


Val Gly Ile Arg Gly Tyr Met Tyr Leu Lys Gly Pro Arg Gly Ser Val 
            260                 265                 270         


Met Thr Thr Asn Ile Tyr Leu Asn Ser Ser Leu Tyr Arg Gly Thr Lys 
        275                 280                 285             


Phe Ile Ile Lys Lys Tyr Ala Ser Gly Asn Lys Asp Asn Ile Val Arg 
    290                 295                 300                 


Asn Asn Asp Arg Val Tyr Ile Asn Val Val Val Lys Asn Lys Glu Tyr 
305                 310                 315                 320 


Arg Leu Ala Thr Asn Ala Ser Gln Ala Gly Val Glu Lys Ile Leu Ser 
                325                 330                 335     


Ala Leu Glu Ile Pro Asp Val Gly Asn Leu Ser Gln Val Val Val Met 
            340                 345                 350         


Lys Ser Lys Asn Asp Lys Gly Ile Thr Asn Lys Cys Lys Met Asn Leu 
        355                 360                 365             


Gln Asp Asn Asn Gly Asn Asp Ile Gly Phe Ile Gly Phe His Gln Phe 
    370                 375                 380                 


Asn Asn Ile Ala Lys Leu Val Ala Ser Asn Trp Tyr Asn Arg Gln Ile 
385                 390                 395                 400 


Glu Arg Ser Ser Arg Thr Leu Gly Cys Ser Trp Glu Phe Ile Pro Val 
                405                 410                 415     


Asp Asp Gly Trp Gly Glu Arg Pro Leu Lys Leu Ala Ala Ala Leu Glu 
            420                 425                 430         


His His His His His His 
        435             


<210>  43
<211>  1341
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide Sequence of rHC/AB (His-tagged)

<400>  43
atgattctga acaatattat cctgaacctg cgttacaaag acaacaatct gatcgatctg       60

agcggctatg gtgcaaaagt tgaagtctac gacggtgtcg aactgaacga taaaaaccag      120

ttcaaactga cctcatcggc taactcaaaa attcgtgtga cgcagaacca aaacatcatc      180

ttcaactcgg tctttctgga cttcagcgtg tctttctgga ttcgcatccc gaaatataaa      240

aatgatggca tccagaacta catccataac gaatacacca tcatcaactg tatgaaaaac      300

aacagtggtt ggaaaatttc catccgtggc aaccgcatta tctggaccct gattgatatc      360

aatggtaaaa cgaaaagcgt gtttttcgaa tacaacatcc gtgaagatat ctctgaatac      420

atcaatcgct ggtttttcgt gaccattacg aacaatctga acaatgcgaa aatctatatc      480

aacggcaaac tggaaagtaa taccgacatc aaagatattc gtgaagttat cgccaacggt      540

gaaatcatct tcaaactgga tggcgacatc gatcgcaccc agttcatttg gatgaaatac      600

ttctccatct tcaacacgga actgagtcag tccaatatcg aagaacgcta caaaatccaa      660

tcatactcgg aatacctgaa agatttctgg ggtaacccgc tgatgtacaa caaagaatac      720

tacatgttca acgcgggcaa caaaaactca tacatcaaac tgaaaaaaga ttcgccggtg      780

ggtgaaatcc tgacccgtag caaatacaac cagaactcta aatacatcaa ctatcgcgat      840

ctgtacattg gcgaaaaatt tattatccgt cgcaaaagca actctcagag tattaatgat      900

gacatcgtgc gtaaagaaga ctacatctat ctggatttct ttaatctgaa ccaagaatgg      960

cgcgtttata cctacaaata cttcaaaaaa gaagaaatga aactgttcct ggccccgatt     1020

tacgacagcg atgaatttta caacaccatc cagatcaaag aatacgatga acagccgacg     1080

tatagttgcc aactgctgtt caaaaaagac gaagaatcca ccgatgaaat tggcctgatt     1140

ggtatccacc gtttctatga aagcggtatc gttttcgaag aatacaaaga ttacttctgt     1200

atctctaaat ggtatctgaa agaagtcaaa cgcaaaccgt acaacctgaa actgggctgc     1260

aactggcaat ttatcccgaa agacgaaggc tggaccgaaa agcttgcggc cgcactcgag     1320

caccaccacc accaccactg a                                               1341


<210>  44
<211>  446
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide Sequence of rHC/AB (His-tagged)

<400>  44

Met Ile Leu Asn Asn Ile Ile Leu Asn Leu Arg Tyr Lys Asp Asn Asn 
1               5                   10                  15      


Leu Ile Asp Leu Ser Gly Tyr Gly Ala Lys Val Glu Val Tyr Asp Gly 
            20                  25                  30          


Val Glu Leu Asn Asp Lys Asn Gln Phe Lys Leu Thr Ser Ser Ala Asn 
        35                  40                  45              


Ser Lys Ile Arg Val Thr Gln Asn Gln Asn Ile Ile Phe Asn Ser Val 
    50                  55                  60                  


Phe Leu Asp Phe Ser Val Ser Phe Trp Ile Arg Ile Pro Lys Tyr Lys 
65                  70                  75                  80  


Asn Asp Gly Ile Gln Asn Tyr Ile His Asn Glu Tyr Thr Ile Ile Asn 
                85                  90                  95      


Cys Met Lys Asn Asn Ser Gly Trp Lys Ile Ser Ile Arg Gly Asn Arg 
            100                 105                 110         


Ile Ile Trp Thr Leu Ile Asp Ile Asn Gly Lys Thr Lys Ser Val Phe 
        115                 120                 125             


Phe Glu Tyr Asn Ile Arg Glu Asp Ile Ser Glu Tyr Ile Asn Arg Trp 
    130                 135                 140                 


Phe Phe Val Thr Ile Thr Asn Asn Leu Asn Asn Ala Lys Ile Tyr Ile 
145                 150                 155                 160 


Asn Gly Lys Leu Glu Ser Asn Thr Asp Ile Lys Asp Ile Arg Glu Val 
                165                 170                 175     


Ile Ala Asn Gly Glu Ile Ile Phe Lys Leu Asp Gly Asp Ile Asp Arg 
            180                 185                 190         


Thr Gln Phe Ile Trp Met Lys Tyr Phe Ser Ile Phe Asn Thr Glu Leu 
        195                 200                 205             


Ser Gln Ser Asn Ile Glu Glu Arg Tyr Lys Ile Gln Ser Tyr Ser Glu 
    210                 215                 220                 


Tyr Leu Lys Asp Phe Trp Gly Asn Pro Leu Met Tyr Asn Lys Glu Tyr 
225                 230                 235                 240 


Tyr Met Phe Asn Ala Gly Asn Lys Asn Ser Tyr Ile Lys Leu Lys Lys 
                245                 250                 255     


Asp Ser Pro Val Gly Glu Ile Leu Thr Arg Ser Lys Tyr Asn Gln Asn 
            260                 265                 270         


Ser Lys Tyr Ile Asn Tyr Arg Asp Leu Tyr Ile Gly Glu Lys Phe Ile 
        275                 280                 285             


Ile Arg Arg Lys Ser Asn Ser Gln Ser Ile Asn Asp Asp Ile Val Arg 
    290                 295                 300                 


Lys Glu Asp Tyr Ile Tyr Leu Asp Phe Phe Asn Leu Asn Gln Glu Trp 
305                 310                 315                 320 


Arg Val Tyr Thr Tyr Lys Tyr Phe Lys Lys Glu Glu Met Lys Leu Phe 
                325                 330                 335     


Leu Ala Pro Ile Tyr Asp Ser Asp Glu Phe Tyr Asn Thr Ile Gln Ile 
            340                 345                 350         


Lys Glu Tyr Asp Glu Gln Pro Thr Tyr Ser Cys Gln Leu Leu Phe Lys 
        355                 360                 365             


Lys Asp Glu Glu Ser Thr Asp Glu Ile Gly Leu Ile Gly Ile His Arg 
    370                 375                 380                 


Phe Tyr Glu Ser Gly Ile Val Phe Glu Glu Tyr Lys Asp Tyr Phe Cys 
385                 390                 395                 400 


Ile Ser Lys Trp Tyr Leu Lys Glu Val Lys Arg Lys Pro Tyr Asn Leu 
                405                 410                 415     


Lys Leu Gly Cys Asn Trp Gln Phe Ile Pro Lys Asp Glu Gly Trp Thr 
            420                 425                 430         


Glu Lys Leu Ala Ala Ala Leu Glu His His His His His His 
        435                 440                 445     


<210>  45
<211>  1306
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide Sequence of rHC/A Variant Y1117V H1253K (His-tagged)

<400>  45
atgatcatca atactagcat tctgaacctg cgttacgaga gcaatcatct gattgatctg       60

agccgttatg caagcaagat caacatcggt agcaaggtca attttgaccc gatcgataag      120

aaccagatcc agctgtttaa tctggaatcg agcaaaattg aggttatcct gaaaaacgcc      180

attgtctaca actccatgta cgagaatttc tccaccagct tctggattcg catcccgaaa      240

tacttcaaca gcattagcct gaacaacgag tatactatca tcaactgtat ggagaacaac      300

agcggttgga aggtgtctct gaactatggt gagatcattt ggaccttgca ggacacccaa      360

gagatcaagc agcgcgtcgt gttcaagtac tctcaaatga tcaacatttc cgattacatt      420

aatcgttgga tcttcgtgac cattacgaat aaccgtctga ataacagcaa gatttacatc      480

aatggtcgct tgatcgatca gaaaccgatt agcaacctgg gtaatatcca cgcaagcaac      540

aacattatgt tcaaattgga cggttgccgc gatacccatc gttatatctg gatcaagtat      600

ttcaacctgt ttgataaaga actgaatgag aaggagatca aagatttgta tgacaaccaa      660

tctaacagcg gcattttgaa ggacttctgg ggcgattatc tgcaatacga taagccgtac      720

tatatgctga acctggttga tccgaacaaa tatgtggatg tcaataatgt gggtattcgt      780

ggttacatgt atttgaaggg tccgcgtggc agcgttatga cgaccaacat ttacctgaac      840

tctagcctgt accgtggtac gaaattcatc attaagaaat atgccagcgg caacaaagat      900

aacattgtgc gtaataacga tcgtgtctac atcaacgtgg tcgtgaagaa taaagagtac      960

cgtctggcga ccaacgcttc gcaggcgggt gttgagaaaa ttctgagcgc gttggagatc     1020

cctgatgtcg gtaatctgag ccaagtcgtg gttatgaaga gcaagaacga ccagggtatc     1080

actaacaagt gcaagatgaa cctgcaagac aacaatggta acgacatcgg ctttattggt     1140

ttcaaacagt tcaacaatat tgctaaactg gtagcgagca attggtacaa tcgtcagatt     1200

gagcgcagca gccgtacttt gggctgtagc tgggagttta tcccggtcga tgatggttgg     1260

ggcgaacgtc cgctgcacca tcaccatcac catcaccatc accatt                    1306


<210>  46
<211>  435
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide Sequence of rHC/A Variant Y1117V H1253K (His-tagged)

<400>  46

Met Ile Ile Asn Thr Ser Ile Leu Asn Leu Arg Tyr Glu Ser Asn His 
1               5                   10                  15      


Leu Ile Asp Leu Ser Arg Tyr Ala Ser Lys Ile Asn Ile Gly Ser Lys 
            20                  25                  30          


Val Asn Phe Asp Pro Ile Asp Lys Asn Gln Ile Gln Leu Phe Asn Leu 
        35                  40                  45              


Glu Ser Ser Lys Ile Glu Val Ile Leu Lys Asn Ala Ile Val Tyr Asn 
    50                  55                  60                  


Ser Met Tyr Glu Asn Phe Ser Thr Ser Phe Trp Ile Arg Ile Pro Lys 
65                  70                  75                  80  


Tyr Phe Asn Ser Ile Ser Leu Asn Asn Glu Tyr Thr Ile Ile Asn Cys 
                85                  90                  95      


Met Glu Asn Asn Ser Gly Trp Lys Val Ser Leu Asn Tyr Gly Glu Ile 
            100                 105                 110         


Ile Trp Thr Leu Gln Asp Thr Gln Glu Ile Lys Gln Arg Val Val Phe 
        115                 120                 125             


Lys Tyr Ser Gln Met Ile Asn Ile Ser Asp Tyr Ile Asn Arg Trp Ile 
    130                 135                 140                 


Phe Val Thr Ile Thr Asn Asn Arg Leu Asn Asn Ser Lys Ile Tyr Ile 
145                 150                 155                 160 


Asn Gly Arg Leu Ile Asp Gln Lys Pro Ile Ser Asn Leu Gly Asn Ile 
                165                 170                 175     


His Ala Ser Asn Asn Ile Met Phe Lys Leu Asp Gly Cys Arg Asp Thr 
            180                 185                 190         


His Arg Tyr Ile Trp Ile Lys Tyr Phe Asn Leu Phe Asp Lys Glu Leu 
        195                 200                 205             


Asn Glu Lys Glu Ile Lys Asp Leu Tyr Asp Asn Gln Ser Asn Ser Gly 
    210                 215                 220                 


Ile Leu Lys Asp Phe Trp Gly Asp Tyr Leu Gln Tyr Asp Lys Pro Tyr 
225                 230                 235                 240 


Tyr Met Leu Asn Leu Val Asp Pro Asn Lys Tyr Val Asp Val Asn Asn 
                245                 250                 255     


Val Gly Ile Arg Gly Tyr Met Tyr Leu Lys Gly Pro Arg Gly Ser Val 
            260                 265                 270         


Met Thr Thr Asn Ile Tyr Leu Asn Ser Ser Leu Tyr Arg Gly Thr Lys 
        275                 280                 285             


Phe Ile Ile Lys Lys Tyr Ala Ser Gly Asn Lys Asp Asn Ile Val Arg 
    290                 295                 300                 


Asn Asn Asp Arg Val Tyr Ile Asn Val Val Val Lys Asn Lys Glu Tyr 
305                 310                 315                 320 


Arg Leu Ala Thr Asn Ala Ser Gln Ala Gly Val Glu Lys Ile Leu Ser 
                325                 330                 335     


Ala Leu Glu Ile Pro Asp Val Gly Asn Leu Ser Gln Val Val Val Met 
            340                 345                 350         


Lys Ser Lys Asn Asp Gln Gly Ile Thr Asn Lys Cys Lys Met Asn Leu 
        355                 360                 365             


Gln Asp Asn Asn Gly Asn Asp Ile Gly Phe Ile Gly Phe Lys Gln Phe 
    370                 375                 380                 


Asn Asn Ile Ala Lys Leu Val Ala Ser Asn Trp Tyr Asn Arg Gln Ile 
385                 390                 395                 400 


Glu Arg Ser Ser Arg Thr Leu Gly Cys Ser Trp Glu Phe Ile Pro Val 
                405                 410                 415     


Asp Asp Gly Trp Gly Glu Arg Pro Leu His His His His His His His 
            420                 425                 430         


His His His 
        435 


<210>  47
<211>  1308
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide Sequence of rHC/A Variant Y1117V F1252Y H1253K L1278F 
       (His-tagged)

<400>  47
atgatcatca atactagcat tctgaacctg cgttacgaga gcaatcatct gattgatctg       60

agccgttatg caagcaagat caacatcggt agcaaggtca attttgaccc gatcgataag      120

aaccagatcc agctgtttaa tctggaatcg agcaaaattg aggttatcct gaaaaacgcc      180

attgtctaca actccatgta cgagaatttc tccaccagct tctggattcg catcccgaaa      240

tacttcaaca gcattagcct gaacaacgag tatactatca tcaactgtat ggagaacaac      300

agcggttgga aggtgtctct gaactatggt gagatcattt ggaccttgca ggacacccaa      360

gagatcaagc agcgcgtcgt gttcaagtac tctcaaatga tcaacatttc cgattacatt      420

aatcgttgga tcttcgtgac cattacgaat aaccgtctga ataacagcaa gatttacatc      480

aatggtcgct tgatcgatca gaaaccgatt agcaacctgg gtaatatcca cgcaagcaac      540

aacattatgt tcaaattgga cggttgccgc gatacccatc gttatatctg gatcaagtat      600

ttcaacctgt ttgataaaga actgaatgag aaggagatca aagatttgta tgacaaccaa      660

tctaacagcg gcattttgaa ggacttctgg ggcgattatc tgcaatacga taagccgtac      720

tatatgctga acctggttga tccgaacaaa tatgtggatg tcaataatgt gggtattcgt      780

ggttacatgt atttgaaggg tccgcgtggc agcgttatga cgaccaacat ttacctgaac      840

tctagcctgt accgtggtac gaaattcatc attaagaaat atgccagcgg caacaaagat      900

aacattgtgc gtaataacga tcgtgtctac atcaacgtgg tcgtgaagaa taaagagtac      960

cgtctggcga ccaacgcttc gcaggcgggt gttgagaaaa ttctgagcgc gttggagatc     1020

cctgatgtcg gtaatctgag ccaagtcgtg gttatgaaga gcaagaacga ccagggtatc     1080

actaacaagt gcaagatgaa cctgcaagac aacaatggta acgacatcgg ctttattggt     1140

tacaaacagt tcaacaatat tgctaaactg gtagcgagca attggtacaa tcgtcagatt     1200

gagcgcagca gccgtacttt tggctgtagc tgggagttta tcccggtcga tgatggttgg     1260

ggcgaacgtc cgctgcacca tcaccatcac catcaccatc accattaa                  1308


<210>  48
<211>  435
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide Sequence of rHC/A Variant Y1117V F1252Y H1253K L1278F
       (His-tagged)

<400>  48

Met Ile Ile Asn Thr Ser Ile Leu Asn Leu Arg Tyr Glu Ser Asn His 
1               5                   10                  15      


Leu Ile Asp Leu Ser Arg Tyr Ala Ser Lys Ile Asn Ile Gly Ser Lys 
            20                  25                  30          


Val Asn Phe Asp Pro Ile Asp Lys Asn Gln Ile Gln Leu Phe Asn Leu 
        35                  40                  45              


Glu Ser Ser Lys Ile Glu Val Ile Leu Lys Asn Ala Ile Val Tyr Asn 
    50                  55                  60                  


Ser Met Tyr Glu Asn Phe Ser Thr Ser Phe Trp Ile Arg Ile Pro Lys 
65                  70                  75                  80  


Tyr Phe Asn Ser Ile Ser Leu Asn Asn Glu Tyr Thr Ile Ile Asn Cys 
                85                  90                  95      


Met Glu Asn Asn Ser Gly Trp Lys Val Ser Leu Asn Tyr Gly Glu Ile 
            100                 105                 110         


Ile Trp Thr Leu Gln Asp Thr Gln Glu Ile Lys Gln Arg Val Val Phe 
        115                 120                 125             


Lys Tyr Ser Gln Met Ile Asn Ile Ser Asp Tyr Ile Asn Arg Trp Ile 
    130                 135                 140                 


Phe Val Thr Ile Thr Asn Asn Arg Leu Asn Asn Ser Lys Ile Tyr Ile 
145                 150                 155                 160 


Asn Gly Arg Leu Ile Asp Gln Lys Pro Ile Ser Asn Leu Gly Asn Ile 
                165                 170                 175     


His Ala Ser Asn Asn Ile Met Phe Lys Leu Asp Gly Cys Arg Asp Thr 
            180                 185                 190         


His Arg Tyr Ile Trp Ile Lys Tyr Phe Asn Leu Phe Asp Lys Glu Leu 
        195                 200                 205             


Asn Glu Lys Glu Ile Lys Asp Leu Tyr Asp Asn Gln Ser Asn Ser Gly 
    210                 215                 220                 


Ile Leu Lys Asp Phe Trp Gly Asp Tyr Leu Gln Tyr Asp Lys Pro Tyr 
225                 230                 235                 240 


Tyr Met Leu Asn Leu Val Asp Pro Asn Lys Tyr Val Asp Val Asn Asn 
                245                 250                 255     


Val Gly Ile Arg Gly Tyr Met Tyr Leu Lys Gly Pro Arg Gly Ser Val 
            260                 265                 270         


Met Thr Thr Asn Ile Tyr Leu Asn Ser Ser Leu Tyr Arg Gly Thr Lys 
        275                 280                 285             


Phe Ile Ile Lys Lys Tyr Ala Ser Gly Asn Lys Asp Asn Ile Val Arg 
    290                 295                 300                 


Asn Asn Asp Arg Val Tyr Ile Asn Val Val Val Lys Asn Lys Glu Tyr 
305                 310                 315                 320 


Arg Leu Ala Thr Asn Ala Ser Gln Ala Gly Val Glu Lys Ile Leu Ser 
                325                 330                 335     


Ala Leu Glu Ile Pro Asp Val Gly Asn Leu Ser Gln Val Val Val Met 
            340                 345                 350         


Lys Ser Lys Asn Asp Gln Gly Ile Thr Asn Lys Cys Lys Met Asn Leu 
        355                 360                 365             


Gln Asp Asn Asn Gly Asn Asp Ile Gly Phe Ile Gly Tyr Lys Gln Phe 
    370                 375                 380                 


Asn Asn Ile Ala Lys Leu Val Ala Ser Asn Trp Tyr Asn Arg Gln Ile 
385                 390                 395                 400 


Glu Arg Ser Ser Arg Thr Phe Gly Cys Ser Trp Glu Phe Ile Pro Val 
                405                 410                 415     


Asp Asp Gly Trp Gly Glu Arg Pro Leu His His His His His His His 
            420                 425                 430         


His His His 
        435 


<210>  49
<211>  1293
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide Sequence of rHC/A Variant Y1117V F1252Y H1253K L1278H 
       (His-tagged)

<400>  49
atgatcatca atactagcat tctgaacctg cgttacgaga gcaatcatct gattgatctg       60

agccgttatg caagcaagat caacatcggt agcaaggtca attttgaccc gatcgataag      120

aaccagatcc agctgtttaa tctggaatcg agcaaaattg aggttatcct gaaaaacgcc      180

attgtctaca actccatgta cgagaatttc tccaccagct tctggattcg catcccgaaa      240

tacttcaaca gcattagcct gaacaacgag tatactatca tcaactgtat ggagaacaac      300

agcggttgga aggtgtctct gaactatggt gagatcattt ggaccttgca ggacacccaa      360

gagatcaagc agcgcgtcgt gttcaagtac tctcaaatga tcaacatttc cgattacatt      420

aatcgttgga tcttcgtgac cattacgaat aaccgtctga ataacagcaa gatttacatc      480

aatggtcgct tgatcgatca gaaaccgatt agcaacctgg gtaatatcca cgcaagcaac      540

aacattatgt tcaaattgga cggttgccgc gatacccatc gttatatctg gatcaagtat      600

ttcaacctgt ttgataaaga actgaatgag aaggagatca aagatttgta tgacaaccaa      660

tctaacagcg gcattttgaa ggacttctgg ggcgattatc tgcaatacga taagccgtac      720

tatatgctga acctggttga tccgaacaaa tatgtggatg tcaataatgt gggtattcgt      780

ggttacatgt atttgaaggg tccgcgtggc agcgttatga cgaccaacat ttacctgaac      840

tctagcctgt accgtggtac gaaattcatc attaagaaat atgccagcgg caacaaagat      900

aacattgtgc gtaataacga tcgtgtctac atcaacgtgg tcgtgaagaa taaagagtac      960

cgtctggcga ccaacgcttc gcaggcgggt gttgagaaaa ttctgagcgc gttggagatc     1020

cctgatgtcg gtaatctgag ccaagtcgtg gttatgaaga gcaagaacga ccagggtatc     1080

actaacaagt gcaagatgaa cctgcaagac aacaatggta acgacatcgg ctttattggt     1140

tacaaacagt tcaacaatat tgctaaactg gtagcgagca attggtacaa tcgtcagatt     1200

gagcgcagca gccgtactca tggctgtagc tgggagttta tcccggtcga tgatggttgg     1260

ggcgaacgtc cgctgcacca tcaccatcac cat                                  1293


<210>  50
<211>  431
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide Sequence of rHC/A Variant Y1117V F1252Y H1253K L1278H
       (His-tagged)

<400>  50

Met Ile Ile Asn Thr Ser Ile Leu Asn Leu Arg Tyr Glu Ser Asn His 
1               5                   10                  15      


Leu Ile Asp Leu Ser Arg Tyr Ala Ser Lys Ile Asn Ile Gly Ser Lys 
            20                  25                  30          


Val Asn Phe Asp Pro Ile Asp Lys Asn Gln Ile Gln Leu Phe Asn Leu 
        35                  40                  45              


Glu Ser Ser Lys Ile Glu Val Ile Leu Lys Asn Ala Ile Val Tyr Asn 
    50                  55                  60                  


Ser Met Tyr Glu Asn Phe Ser Thr Ser Phe Trp Ile Arg Ile Pro Lys 
65                  70                  75                  80  


Tyr Phe Asn Ser Ile Ser Leu Asn Asn Glu Tyr Thr Ile Ile Asn Cys 
                85                  90                  95      


Met Glu Asn Asn Ser Gly Trp Lys Val Ser Leu Asn Tyr Gly Glu Ile 
            100                 105                 110         


Ile Trp Thr Leu Gln Asp Thr Gln Glu Ile Lys Gln Arg Val Val Phe 
        115                 120                 125             


Lys Tyr Ser Gln Met Ile Asn Ile Ser Asp Tyr Ile Asn Arg Trp Ile 
    130                 135                 140                 


Phe Val Thr Ile Thr Asn Asn Arg Leu Asn Asn Ser Lys Ile Tyr Ile 
145                 150                 155                 160 


Asn Gly Arg Leu Ile Asp Gln Lys Pro Ile Ser Asn Leu Gly Asn Ile 
                165                 170                 175     


His Ala Ser Asn Asn Ile Met Phe Lys Leu Asp Gly Cys Arg Asp Thr 
            180                 185                 190         


His Arg Tyr Ile Trp Ile Lys Tyr Phe Asn Leu Phe Asp Lys Glu Leu 
        195                 200                 205             


Asn Glu Lys Glu Ile Lys Asp Leu Tyr Asp Asn Gln Ser Asn Ser Gly 
    210                 215                 220                 


Ile Leu Lys Asp Phe Trp Gly Asp Tyr Leu Gln Tyr Asp Lys Pro Tyr 
225                 230                 235                 240 


Tyr Met Leu Asn Leu Val Asp Pro Asn Lys Tyr Val Asp Val Asn Asn 
                245                 250                 255     


Val Gly Ile Arg Gly Tyr Met Tyr Leu Lys Gly Pro Arg Gly Ser Val 
            260                 265                 270         


Met Thr Thr Asn Ile Tyr Leu Asn Ser Ser Leu Tyr Arg Gly Thr Lys 
        275                 280                 285             


Phe Ile Ile Lys Lys Tyr Ala Ser Gly Asn Lys Asp Asn Ile Val Arg 
    290                 295                 300                 


Asn Asn Asp Arg Val Tyr Ile Asn Val Val Val Lys Asn Lys Glu Tyr 
305                 310                 315                 320 


Arg Leu Ala Thr Asn Ala Ser Gln Ala Gly Val Glu Lys Ile Leu Ser 
                325                 330                 335     


Ala Leu Glu Ile Pro Asp Val Gly Asn Leu Ser Gln Val Val Val Met 
            340                 345                 350         


Lys Ser Lys Asn Asp Gln Gly Ile Thr Asn Lys Cys Lys Met Asn Leu 
        355                 360                 365             


Gln Asp Asn Asn Gly Asn Asp Ile Gly Phe Ile Gly Tyr Lys Gln Phe 
    370                 375                 380                 


Asn Asn Ile Ala Lys Leu Val Ala Ser Asn Trp Tyr Asn Arg Gln Ile 
385                 390                 395                 400 


Glu Arg Ser Ser Arg Thr His Gly Cys Ser Trp Glu Phe Ile Pro Val 
                405                 410                 415     


Asp Asp Gly Trp Gly Glu Arg Pro Leu His His His His His His 
            420                 425                 430     


<210>  51
<211>  1296
<212>  PRT
<213>  Clostridium botulinum

<400>  51

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Val Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly Tyr Asn Lys 
        435                 440                 445             


Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe 
    450                 455                 460                 


Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu 
465                 470                 475                 480 


Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu 
                485                 490                 495     


Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro 
            500                 505                 510         


Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu 
        515                 520                 525             


Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu 
    530                 535                 540                 


Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu 
545                 550                 555                 560 


His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu 
                565                 570                 575     


Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys 
            580                 585                 590         


Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu 
        595                 600                 605             


Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr 
    610                 615                 620                 


Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala 
625                 630                 635                 640 


Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu 
                645                 650                 655     


Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala 
            660                 665                 670         


Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys 
        675                 680                 685             


Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu 
    690                 695                 700                 


Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys 
705                 710                 715                 720 


Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu 
                725                 730                 735     


Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn 
            740                 745                 750         


Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp 
        755                 760                 765             


Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile 
    770                 775                 780                 


Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met 
785                 790                 795                 800 


Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys 
                805                 810                 815     


Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly 
            820                 825                 830         


Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp 
        835                 840                 845             


Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser 
    850                 855                 860                 


Thr Phe Thr Glu Tyr Ile Lys Asn Ile Ile Asn Thr Ser Ile Leu Asn 
865                 870                 875                 880 


Leu Arg Tyr Glu Ser Asn His Leu Ile Asp Leu Ser Arg Tyr Ala Ser 
                885                 890                 895     


Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp Pro Ile Asp Lys Asn 
            900                 905                 910         


Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys Ile Glu Val Ile Leu 
        915                 920                 925             


Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr Glu Asn Phe Ser Thr Ser 
    930                 935                 940                 


Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn Ser Ile Ser Leu Asn Asn 
945                 950                 955                 960 


Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn Ser Gly Trp Lys Val 
                965                 970                 975     


Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr Leu Gln Asp Thr Gln Glu 
            980                 985                 990         


Ile Lys Gln Arg Val Val Phe Lys  Tyr Ser Gln Met Ile  Asn Ile Ser 
        995                 1000                 1005             


Asp Tyr  Ile Asn Arg Trp Ile  Phe Val Thr Ile Thr  Asn Asn Arg 
    1010                 1015                 1020             


Leu Asn  Asn Ser Lys Ile Tyr  Ile Asn Gly Arg Leu  Ile Asp Gln 
    1025                 1030                 1035             


Lys Pro  Ile Ser Asn Leu Gly  Asn Ile His Ala Ser  Asn Asn Ile 
    1040                 1045                 1050             


Met Phe  Lys Leu Asp Gly Cys  Arg Asp Thr His Arg  Tyr Ile Trp 
    1055                 1060                 1065             


Ile Lys  Tyr Phe Asn Leu Phe  Asp Lys Glu Leu Asn  Glu Lys Glu 
    1070                 1075                 1080             


Ile Lys  Asp Leu Tyr Asp Asn  Gln Ser Asn Ser Gly  Ile Leu Lys 
    1085                 1090                 1095             


Asp Phe  Trp Gly Asp Tyr Leu  Gln Tyr Asp Lys Pro  Tyr Tyr Met 
    1100                 1105                 1110             


Leu Asn  Leu Tyr Asp Pro Asn  Lys Tyr Val Asp Val  Asn Asn Val 
    1115                 1120                 1125             


Gly Ile  Arg Gly Tyr Met Tyr  Leu Lys Gly Pro Arg  Gly Ser Val 
    1130                 1135                 1140             


Met Thr  Thr Asn Ile Tyr Leu  Asn Ser Ser Leu Tyr  Arg Gly Thr 
    1145                 1150                 1155             


Lys Phe  Ile Ile Lys Lys Tyr  Ala Ser Gly Asn Lys  Asp Asn Ile 
    1160                 1165                 1170             


Val Arg  Asn Asn Asp Arg Val  Tyr Ile Asn Val Val  Val Lys Asn 
    1175                 1180                 1185             


Lys Glu  Tyr Arg Leu Ala Thr  Asn Ala Ser Gln Ala  Gly Val Glu 
    1190                 1195                 1200             


Lys Ile  Leu Ser Ala Leu Glu  Ile Pro Asp Val Gly  Asn Leu Ser 
    1205                 1210                 1215             


Gln Val  Val Val Met Lys Ser  Lys Asn Asp Gln Gly  Ile Thr Asn 
    1220                 1225                 1230             


Lys Cys  Lys Met Asn Leu Gln  Asp Asn Asn Gly Asn  Asp Ile Gly 
    1235                 1240                 1245             


Phe Ile  Gly Phe His Gln Phe  Asn Asn Ile Ala Lys  Leu Val Ala 
    1250                 1255                 1260             


Ser Asn  Trp Tyr Asn Arg Gln  Ile Glu Arg Ser Ser  Arg Thr Leu 
    1265                 1270                 1275             


Gly Cys  Ser Trp Glu Phe Ile  Pro Val Asp Asp Gly  Trp Gly Glu 
    1280                 1285                 1290             


Arg Pro  Leu 
    1295     


<210>  52
<211>  1291
<212>  PRT
<213>  Clostridium botulinum

<400>  52

Met Pro Val Thr Ile Asn Asn Phe Asn Tyr Asn Asp Pro Ile Asp Asn 
1               5                   10                  15      


Asn Asn Ile Ile Met Met Glu Pro Pro Phe Ala Arg Gly Thr Gly Arg 
            20                  25                  30          


Tyr Tyr Lys Ala Phe Lys Ile Thr Asp Arg Ile Trp Ile Ile Pro Glu 
        35                  40                  45              


Arg Tyr Thr Phe Gly Tyr Lys Pro Glu Asp Phe Asn Lys Ser Ser Gly 
    50                  55                  60                  


Ile Phe Asn Arg Asp Val Cys Glu Tyr Tyr Asp Pro Asp Tyr Leu Asn 
65                  70                  75                  80  


Thr Asn Asp Lys Lys Asn Ile Phe Leu Gln Thr Met Ile Lys Leu Phe 
                85                  90                  95      


Asn Arg Ile Lys Ser Lys Pro Leu Gly Glu Lys Leu Leu Glu Met Ile 
            100                 105                 110         


Ile Asn Gly Ile Pro Tyr Leu Gly Asp Arg Arg Val Pro Leu Glu Glu 
        115                 120                 125             


Phe Asn Thr Asn Ile Ala Ser Val Thr Val Asn Lys Leu Ile Ser Asn 
    130                 135                 140                 


Pro Gly Glu Val Glu Arg Lys Lys Gly Ile Phe Ala Asn Leu Ile Ile 
145                 150                 155                 160 


Phe Gly Pro Gly Pro Val Leu Asn Glu Asn Glu Thr Ile Asp Ile Gly 
                165                 170                 175     


Ile Gln Asn His Phe Ala Ser Arg Glu Gly Phe Gly Gly Ile Met Gln 
            180                 185                 190         


Met Lys Phe Cys Pro Glu Tyr Val Ser Val Phe Asn Asn Val Gln Glu 
        195                 200                 205             


Asn Lys Gly Ala Ser Ile Phe Asn Arg Arg Gly Tyr Phe Ser Asp Pro 
    210                 215                 220                 


Ala Leu Ile Leu Met His Glu Leu Ile His Val Leu His Gly Leu Tyr 
225                 230                 235                 240 


Gly Ile Lys Val Asp Asp Leu Pro Ile Val Pro Asn Glu Lys Lys Phe 
                245                 250                 255     


Phe Met Gln Ser Thr Asp Ala Ile Gln Ala Glu Glu Leu Tyr Thr Phe 
            260                 265                 270         


Gly Gly Gln Asp Pro Ser Ile Ile Thr Pro Ser Thr Asp Lys Ser Ile 
        275                 280                 285             


Tyr Asp Lys Val Leu Gln Asn Phe Arg Gly Ile Val Asp Arg Leu Asn 
    290                 295                 300                 


Lys Val Leu Val Cys Ile Ser Asp Pro Asn Ile Asn Ile Asn Ile Tyr 
305                 310                 315                 320 


Lys Asn Lys Phe Lys Asp Lys Tyr Lys Phe Val Glu Asp Ser Glu Gly 
                325                 330                 335     


Lys Tyr Ser Ile Asp Val Glu Ser Phe Asp Lys Leu Tyr Lys Ser Leu 
            340                 345                 350         


Met Phe Gly Phe Thr Glu Thr Asn Ile Ala Glu Asn Tyr Lys Ile Lys 
        355                 360                 365             


Thr Arg Ala Ser Tyr Phe Ser Asp Ser Leu Pro Pro Val Lys Ile Lys 
    370                 375                 380                 


Asn Leu Leu Asp Asn Glu Ile Tyr Thr Ile Glu Glu Gly Phe Asn Ile 
385                 390                 395                 400 


Ser Asp Lys Asp Met Glu Lys Glu Tyr Arg Gly Gln Asn Lys Ala Ile 
                405                 410                 415     


Asn Lys Gln Ala Tyr Glu Glu Ile Ser Lys Glu His Leu Ala Val Tyr 
            420                 425                 430         


Lys Ile Gln Met Cys Lys Ser Val Lys Ala Pro Gly Ile Cys Ile Asp 
        435                 440                 445             


Val Asp Asn Glu Asp Leu Phe Phe Ile Ala Asp Lys Asn Ser Phe Ser 
    450                 455                 460                 


Asp Asp Leu Ser Lys Asn Glu Arg Ile Glu Tyr Asn Thr Gln Ser Asn 
465                 470                 475                 480 


Tyr Ile Glu Asn Asp Phe Pro Ile Asn Glu Leu Ile Leu Asp Thr Asp 
                485                 490                 495     


Leu Ile Ser Lys Ile Glu Leu Pro Ser Glu Asn Thr Glu Ser Leu Thr 
            500                 505                 510         


Asp Phe Asn Val Asp Val Pro Val Tyr Glu Lys Gln Pro Ala Ile Lys 
        515                 520                 525             


Lys Ile Phe Thr Asp Glu Asn Thr Ile Phe Gln Tyr Leu Tyr Ser Gln 
    530                 535                 540                 


Thr Phe Pro Leu Asp Ile Arg Asp Ile Ser Leu Thr Ser Ser Phe Asp 
545                 550                 555                 560 


Asp Ala Leu Leu Phe Ser Asn Lys Val Tyr Ser Phe Phe Ser Met Asp 
                565                 570                 575     


Tyr Ile Lys Thr Ala Asn Lys Val Val Glu Ala Gly Leu Phe Ala Gly 
            580                 585                 590         


Trp Val Lys Gln Ile Val Asn Asp Phe Val Ile Glu Ala Asn Lys Ser 
        595                 600                 605             


Asn Thr Met Asp Lys Ile Ala Asp Ile Ser Leu Ile Val Pro Tyr Ile 
    610                 615                 620                 


Gly Leu Ala Leu Asn Val Gly Asn Glu Thr Ala Lys Gly Asn Phe Glu 
625                 630                 635                 640 


Asn Ala Phe Glu Ile Ala Gly Ala Ser Ile Leu Leu Glu Phe Ile Pro 
                645                 650                 655     


Glu Leu Leu Ile Pro Val Val Gly Ala Phe Leu Leu Glu Ser Tyr Ile 
            660                 665                 670         


Asp Asn Lys Asn Lys Ile Ile Lys Thr Ile Asp Asn Ala Leu Thr Lys 
        675                 680                 685             


Arg Asn Glu Lys Trp Ser Asp Met Tyr Gly Leu Ile Val Ala Gln Trp 
    690                 695                 700                 


Leu Ser Thr Val Asn Thr Gln Phe Tyr Thr Ile Lys Glu Gly Met Tyr 
705                 710                 715                 720 


Lys Ala Leu Asn Tyr Gln Ala Gln Ala Leu Glu Glu Ile Ile Lys Tyr 
                725                 730                 735     


Arg Tyr Asn Ile Tyr Ser Glu Lys Glu Lys Ser Asn Ile Asn Ile Asp 
            740                 745                 750         


Phe Asn Asp Ile Asn Ser Lys Leu Asn Glu Gly Ile Asn Gln Ala Ile 
        755                 760                 765             


Asp Asn Ile Asn Asn Phe Ile Asn Gly Cys Ser Val Ser Tyr Leu Met 
    770                 775                 780                 


Lys Lys Met Ile Pro Leu Ala Val Glu Lys Leu Leu Asp Phe Asp Asn 
785                 790                 795                 800 


Thr Leu Lys Lys Asn Leu Leu Asn Tyr Ile Asp Glu Asn Lys Leu Tyr 
                805                 810                 815     


Leu Ile Gly Ser Ala Glu Tyr Glu Lys Ser Lys Val Asn Lys Tyr Leu 
            820                 825                 830         


Lys Thr Ile Met Pro Phe Asp Leu Ser Ile Tyr Thr Asn Asp Thr Ile 
        835                 840                 845             


Leu Ile Glu Met Phe Asn Lys Tyr Asn Ser Glu Ile Leu Asn Asn Ile 
    850                 855                 860                 


Ile Leu Asn Leu Arg Tyr Lys Asp Asn Asn Leu Ile Asp Leu Ser Gly 
865                 870                 875                 880 


Tyr Gly Ala Lys Val Glu Val Tyr Asp Gly Val Glu Leu Asn Asp Lys 
                885                 890                 895     


Asn Gln Phe Lys Leu Thr Ser Ser Ala Asn Ser Lys Ile Arg Val Thr 
            900                 905                 910         


Gln Asn Gln Asn Ile Ile Phe Asn Ser Val Phe Leu Asp Phe Ser Val 
        915                 920                 925             


Ser Phe Trp Ile Arg Ile Pro Lys Tyr Lys Asn Asp Gly Ile Gln Asn 
    930                 935                 940                 


Tyr Ile His Asn Glu Tyr Thr Ile Ile Asn Cys Met Lys Asn Asn Ser 
945                 950                 955                 960 


Gly Trp Lys Ile Ser Ile Arg Gly Asn Arg Ile Ile Trp Thr Leu Ile 
                965                 970                 975     


Asp Ile Asn Gly Lys Thr Lys Ser Val Phe Phe Glu Tyr Asn Ile Arg 
            980                 985                 990         


Glu Asp Ile Ser Glu Tyr Ile Asn  Arg Trp Phe Phe Val  Thr Ile Thr 
        995                 1000                 1005             


Asn Asn  Leu Asn Asn Ala Lys  Ile Tyr Ile Asn Gly  Lys Leu Glu 
    1010                 1015                 1020             


Ser Asn  Thr Asp Ile Lys Asp  Ile Arg Glu Val Ile  Ala Asn Gly 
    1025                 1030                 1035             


Glu Ile  Ile Phe Lys Leu Asp  Gly Asp Ile Asp Arg  Thr Gln Phe 
    1040                 1045                 1050             


Ile Trp  Met Lys Tyr Phe Ser  Ile Phe Asn Thr Glu  Leu Ser Gln 
    1055                 1060                 1065             


Ser Asn  Ile Glu Glu Arg Tyr  Lys Ile Gln Ser Tyr  Ser Glu Tyr 
    1070                 1075                 1080             


Leu Lys  Asp Phe Trp Gly Asn  Pro Leu Met Tyr Asn  Lys Glu Tyr 
    1085                 1090                 1095             


Tyr Met  Phe Asn Ala Gly Asn  Lys Asn Ser Tyr Ile  Lys Leu Lys 
    1100                 1105                 1110             


Lys Asp  Ser Pro Val Gly Glu  Ile Leu Thr Arg Ser  Lys Tyr Asn 
    1115                 1120                 1125             


Gln Asn  Ser Lys Tyr Ile Asn  Tyr Arg Asp Leu Tyr  Ile Gly Glu 
    1130                 1135                 1140             


Lys Phe  Ile Ile Arg Arg Lys  Ser Asn Ser Gln Ser  Ile Asn Asp 
    1145                 1150                 1155             


Asp Ile  Val Arg Lys Glu Asp  Tyr Ile Tyr Leu Asp  Phe Phe Asn 
    1160                 1165                 1170             


Leu Asn  Gln Glu Trp Arg Val  Tyr Thr Tyr Lys Tyr  Phe Lys Lys 
    1175                 1180                 1185             


Glu Glu  Glu Lys Leu Phe Leu  Ala Pro Ile Ser Asp  Ser Asp Glu 
    1190                 1195                 1200             


Phe Tyr  Asn Thr Ile Gln Ile  Lys Glu Tyr Asp Glu  Gln Pro Thr 
    1205                 1210                 1215             


Tyr Ser  Cys Gln Leu Leu Phe  Lys Lys Asp Glu Glu  Ser Thr Asp 
    1220                 1225                 1230             


Glu Ile  Gly Leu Ile Gly Ile  His Arg Phe Tyr Glu  Ser Gly Ile 
    1235                 1240                 1245             


Val Phe  Glu Glu Tyr Lys Asp  Tyr Phe Cys Ile Ser  Lys Trp Tyr 
    1250                 1255                 1260             


Leu Lys  Glu Val Lys Arg Lys  Pro Tyr Asn Leu Lys  Leu Gly Cys 
    1265                 1270                 1275             


Asn Trp  Gln Phe Ile Pro Lys  Asp Glu Gly Trp Thr  Glu 
    1280                 1285                 1290     


<210>  53
<211>  1291
<212>  PRT
<213>  Clostridium botulinum

<400>  53

Met Pro Ile Thr Ile Asn Asn Phe Asn Tyr Ser Asp Pro Val Asp Asn 
1               5                   10                  15      


Lys Asn Ile Leu Tyr Leu Asp Thr His Leu Asn Thr Leu Ala Asn Glu 
            20                  25                  30          


Pro Glu Lys Ala Phe Arg Ile Thr Gly Asn Ile Trp Val Ile Pro Asp 
        35                  40                  45              


Arg Phe Ser Arg Asn Ser Asn Pro Asn Leu Asn Lys Pro Pro Arg Val 
    50                  55                  60                  


Thr Ser Pro Lys Ser Gly Tyr Tyr Asp Pro Asn Tyr Leu Ser Thr Asp 
65                  70                  75                  80  


Ser Asp Lys Asp Pro Phe Leu Lys Glu Ile Ile Lys Leu Phe Lys Arg 
                85                  90                  95      


Ile Asn Ser Arg Glu Ile Gly Glu Glu Leu Ile Tyr Arg Leu Ser Thr 
            100                 105                 110         


Asp Ile Pro Phe Pro Gly Asn Asn Asn Thr Pro Ile Asn Thr Phe Asp 
        115                 120                 125             


Phe Asp Val Asp Phe Asn Ser Val Asp Val Lys Thr Arg Gln Gly Asn 
    130                 135                 140                 


Asn Trp Val Lys Thr Gly Ser Ile Asn Pro Ser Val Ile Ile Thr Gly 
145                 150                 155                 160 


Pro Arg Glu Asn Ile Ile Asp Pro Glu Thr Ser Thr Phe Lys Leu Thr 
                165                 170                 175     


Asn Asn Thr Phe Ala Ala Gln Glu Gly Phe Gly Ala Leu Ser Ile Ile 
            180                 185                 190         


Ser Ile Ser Pro Arg Phe Met Leu Thr Tyr Ser Asn Ala Thr Asn Asp 
        195                 200                 205             


Val Gly Glu Gly Arg Phe Ser Lys Ser Glu Phe Cys Met Asp Pro Ile 
    210                 215                 220                 


Leu Ile Leu Met His Glu Leu Asn His Ala Met His Asn Leu Tyr Gly 
225                 230                 235                 240 


Ile Ala Ile Pro Asn Asp Gln Thr Ile Ser Ser Val Thr Ser Asn Ile 
                245                 250                 255     


Phe Tyr Ser Gln Tyr Asn Val Lys Leu Glu Tyr Ala Glu Ile Tyr Ala 
            260                 265                 270         


Phe Gly Gly Pro Thr Ile Asp Leu Ile Pro Lys Ser Ala Arg Lys Tyr 
        275                 280                 285             


Phe Glu Glu Lys Ala Leu Asp Tyr Tyr Arg Ser Ile Ala Lys Arg Leu 
    290                 295                 300                 


Asn Ser Ile Thr Thr Ala Asn Pro Ser Ser Phe Asn Lys Tyr Ile Gly 
305                 310                 315                 320 


Glu Tyr Lys Gln Lys Leu Ile Arg Lys Tyr Arg Phe Val Val Glu Ser 
                325                 330                 335     


Ser Gly Glu Val Thr Val Asn Arg Asn Lys Phe Val Glu Leu Tyr Asn 
            340                 345                 350         


Glu Leu Thr Gln Ile Phe Thr Glu Phe Asn Tyr Ala Lys Ile Tyr Asn 
        355                 360                 365             


Val Gln Asn Arg Lys Ile Tyr Leu Ser Asn Val Tyr Thr Pro Val Thr 
    370                 375                 380                 


Ala Asn Ile Leu Asp Asp Asn Val Tyr Asp Ile Gln Asn Gly Phe Asn 
385                 390                 395                 400 


Ile Pro Lys Ser Asn Leu Asn Val Leu Phe Met Gly Gln Asn Leu Ser 
                405                 410                 415     


Arg Asn Pro Ala Leu Arg Lys Val Asn Pro Glu Asn Met Leu Tyr Leu 
            420                 425                 430         


Phe Thr Lys Phe Cys His Lys Ala Ile Asp Gly Arg Ser Leu Tyr Asn 
        435                 440                 445             


Lys Thr Leu Asp Cys Arg Glu Leu Leu Val Lys Asn Thr Asp Leu Pro 
    450                 455                 460                 


Phe Ile Gly Asp Ile Ser Asp Val Lys Thr Asp Ile Phe Leu Arg Lys 
465                 470                 475                 480 


Asp Ile Asn Glu Glu Thr Glu Val Ile Tyr Tyr Pro Asp Asn Val Ser 
                485                 490                 495     


Val Asp Gln Val Ile Leu Ser Lys Asn Thr Ser Glu His Gly Gln Leu 
            500                 505                 510         


Asp Leu Leu Tyr Pro Ser Ile Asp Ser Glu Ser Glu Ile Leu Pro Gly 
        515                 520                 525             


Glu Asn Gln Val Phe Tyr Asp Asn Arg Thr Gln Asn Val Asp Tyr Leu 
    530                 535                 540                 


Asn Ser Tyr Tyr Tyr Leu Glu Ser Gln Lys Leu Ser Asp Asn Val Glu 
545                 550                 555                 560 


Asp Phe Thr Phe Thr Arg Ser Ile Glu Glu Ala Leu Asp Asn Ser Ala 
                565                 570                 575     


Lys Val Tyr Thr Tyr Phe Pro Thr Leu Ala Asn Lys Val Asn Ala Gly 
            580                 585                 590         


Val Gln Gly Gly Leu Phe Leu Met Trp Ala Asn Asp Val Val Glu Asp 
        595                 600                 605             


Phe Thr Thr Asn Ile Leu Arg Lys Asp Thr Leu Asp Lys Ile Ser Asp 
    610                 615                 620                 


Val Ser Ala Ile Ile Pro Tyr Ile Gly Pro Ala Leu Asn Ile Ser Asn 
625                 630                 635                 640 


Ser Val Arg Arg Gly Asn Phe Thr Glu Ala Phe Ala Val Thr Gly Val 
                645                 650                 655     


Thr Ile Leu Leu Glu Ala Phe Pro Glu Phe Thr Ile Pro Ala Leu Gly 
            660                 665                 670         


Ala Phe Val Ile Tyr Ser Lys Val Gln Glu Arg Asn Glu Ile Ile Lys 
        675                 680                 685             


Thr Ile Asp Asn Cys Leu Glu Gln Arg Ile Lys Arg Trp Lys Asp Ser 
    690                 695                 700                 


Tyr Glu Trp Met Met Gly Thr Trp Leu Ser Arg Ile Ile Thr Gln Phe 
705                 710                 715                 720 


Asn Asn Ile Ser Tyr Gln Met Tyr Asp Ser Leu Asn Tyr Gln Ala Gly 
                725                 730                 735     


Ala Ile Lys Ala Lys Ile Asp Leu Glu Tyr Lys Lys Tyr Ser Gly Ser 
            740                 745                 750         


Asp Lys Glu Asn Ile Lys Ser Gln Val Glu Asn Leu Lys Asn Ser Leu 
        755                 760                 765             


Asp Val Lys Ile Ser Glu Ala Met Asn Asn Ile Asn Lys Phe Ile Arg 
    770                 775                 780                 


Glu Cys Ser Val Thr Tyr Leu Phe Lys Asn Met Leu Pro Lys Val Ile 
785                 790                 795                 800 


Asp Glu Leu Asn Glu Phe Asp Arg Asn Thr Lys Ala Lys Leu Ile Asn 
                805                 810                 815     


Leu Ile Asp Ser His Asn Ile Ile Leu Val Gly Glu Val Asp Lys Leu 
            820                 825                 830         


Lys Ala Lys Val Asn Asn Ser Phe Gln Asn Thr Ile Pro Phe Asn Ile 
        835                 840                 845             


Phe Ser Tyr Thr Asn Asn Ser Leu Leu Lys Asp Ile Ile Asn Glu Tyr 
    850                 855                 860                 


Phe Asn Asn Ile Asn Asp Ser Lys Ile Leu Ser Leu Gln Asn Arg Lys 
865                 870                 875                 880 


Asn Thr Leu Val Asp Thr Ser Gly Tyr Asn Ala Glu Val Ser Glu Glu 
                885                 890                 895     


Gly Asp Val Gln Leu Asn Pro Ile Phe Pro Phe Asp Phe Lys Leu Gly 
            900                 905                 910         


Ser Ser Gly Glu Asp Arg Gly Lys Val Ile Val Thr Gln Asn Glu Asn 
        915                 920                 925             


Ile Val Tyr Asn Ser Met Tyr Glu Ser Phe Ser Ile Ser Phe Trp Ile 
    930                 935                 940                 


Arg Ile Asn Lys Trp Val Ser Asn Leu Pro Gly Tyr Thr Ile Ile Asp 
945                 950                 955                 960 


Ser Val Lys Asn Asn Ser Gly Trp Ser Ile Gly Ile Ile Ser Asn Phe 
                965                 970                 975     


Leu Val Phe Thr Leu Lys Gln Asn Glu Asp Ser Glu Gln Ser Ile Asn 
            980                 985                 990         


Phe Ser Tyr Asp Ile Ser Asn Asn  Ala Pro Gly Tyr Asn  Lys Trp Phe 
        995                 1000                 1005             


Phe Val  Thr Val Thr Asn Asn  Met Met Gly Asn Met  Lys Ile Tyr 
    1010                 1015                 1020             


Ile Asn  Gly Lys Leu Ile Asp  Thr Ile Lys Val Lys  Glu Leu Thr 
    1025                 1030                 1035             


Gly Ile  Asn Phe Ser Lys Thr  Ile Thr Phe Glu Ile  Asn Lys Ile 
    1040                 1045                 1050             


Pro Asp  Thr Gly Leu Ile Thr  Ser Asp Ser Asp Asn  Ile Asn Met 
    1055                 1060                 1065             


Trp Ile  Arg Asp Phe Tyr Ile  Phe Ala Lys Glu Leu  Asp Gly Lys 
    1070                 1075                 1080             


Asp Ile  Asn Ile Leu Phe Asn  Ser Leu Gln Tyr Thr  Asn Val Val 
    1085                 1090                 1095             


Lys Asp  Tyr Trp Gly Asn Asp  Leu Arg Tyr Asn Lys  Glu Tyr Tyr 
    1100                 1105                 1110             


Met Val  Asn Ile Asp Tyr Leu  Asn Arg Tyr Met Tyr  Ala Asn Ser 
    1115                 1120                 1125             


Arg Gln  Ile Val Phe Asn Thr  Arg Arg Asn Asn Asn  Asp Phe Asn 
    1130                 1135                 1140             


Glu Gly  Tyr Lys Ile Ile Ile  Lys Arg Ile Arg Gly  Asn Thr Asn 
    1145                 1150                 1155             


Asp Thr  Arg Val Arg Gly Gly  Asp Ile Leu Tyr Phe  Asp Met Thr 
    1160                 1165                 1170             


Ile Asn  Asn Lys Ala Tyr Asn  Leu Phe Met Lys Asn  Glu Thr Met 
    1175                 1180                 1185             


Tyr Ala  Asp Asn His Ser Thr  Glu Asp Ile Tyr Ala  Ile Gly Leu 
    1190                 1195                 1200             


Arg Glu  Gln Thr Lys Asp Ile  Asn Asp Asn Ile Ile  Phe Gln Ile 
    1205                 1210                 1215             


Gln Pro  Met Asn Asn Thr Tyr  Tyr Tyr Ala Ser Gln  Ile Phe Lys 
    1220                 1225                 1230             


Ser Asn  Phe Asn Gly Glu Asn  Ile Ser Gly Ile Cys  Ser Ile Gly 
    1235                 1240                 1245             


Thr Tyr  Arg Phe Arg Leu Gly  Gly Asp Trp Tyr Arg  His Asn Tyr 
    1250                 1255                 1260             


Leu Val  Pro Thr Val Lys Gln  Gly Asn Tyr Ala Ser  Leu Leu Glu 
    1265                 1270                 1275             


Ser Thr  Ser Thr His Trp Gly  Phe Val Pro Val Ser  Glu 
    1280                 1285                 1290     


<210>  54
<211>  1276
<212>  PRT
<213>  Clostridium botulinum

<400>  54

Met Thr Trp Pro Val Lys Asp Phe Asn Tyr Ser Asp Pro Val Asn Asp 
1               5                   10                  15      


Asn Asp Ile Leu Tyr Leu Arg Ile Pro Gln Asn Lys Leu Ile Thr Thr 
            20                  25                  30          


Pro Val Lys Ala Phe Met Ile Thr Gln Asn Ile Trp Val Ile Pro Glu 
        35                  40                  45              


Arg Phe Ser Ser Asp Thr Asn Pro Ser Leu Ser Lys Pro Pro Arg Pro 
    50                  55                  60                  


Thr Ser Lys Tyr Gln Ser Tyr Tyr Asp Pro Ser Tyr Leu Ser Thr Asp 
65                  70                  75                  80  


Glu Gln Lys Asp Thr Phe Leu Lys Gly Ile Ile Lys Leu Phe Lys Arg 
                85                  90                  95      


Ile Asn Glu Arg Asp Ile Gly Lys Lys Leu Ile Asn Tyr Leu Val Val 
            100                 105                 110         


Gly Ser Pro Phe Met Gly Asp Ser Ser Thr Pro Glu Asp Thr Phe Asp 
        115                 120                 125             


Phe Thr Arg His Thr Thr Asn Ile Ala Val Glu Lys Phe Glu Asn Gly 
    130                 135                 140                 


Ser Trp Lys Val Thr Asn Ile Ile Thr Pro Ser Val Leu Ile Phe Gly 
145                 150                 155                 160 


Pro Leu Pro Asn Ile Leu Asp Tyr Thr Ala Ser Leu Thr Leu Gln Gly 
                165                 170                 175     


Gln Gln Ser Asn Pro Ser Phe Glu Gly Phe Gly Thr Leu Ser Ile Leu 
            180                 185                 190         


Lys Val Ala Pro Glu Phe Leu Leu Thr Phe Ser Asp Val Thr Ser Asn 
        195                 200                 205             


Gln Ser Ser Ala Val Leu Gly Lys Ser Ile Phe Cys Met Asp Pro Val 
    210                 215                 220                 


Ile Ala Leu Met His Glu Leu Thr His Ser Leu His Gln Leu Tyr Gly 
225                 230                 235                 240 


Ile Asn Ile Pro Ser Asp Lys Arg Ile Arg Pro Gln Val Ser Glu Gly 
                245                 250                 255     


Phe Phe Ser Gln Asp Gly Pro Asn Val Gln Phe Glu Glu Leu Tyr Thr 
            260                 265                 270         


Phe Gly Gly Leu Asp Val Glu Ile Ile Pro Gln Ile Glu Arg Ser Gln 
        275                 280                 285             


Leu Arg Glu Lys Ala Leu Gly His Tyr Lys Asp Ile Ala Lys Arg Leu 
    290                 295                 300                 


Asn Asn Ile Asn Lys Thr Ile Pro Ser Ser Trp Ile Ser Asn Ile Asp 
305                 310                 315                 320 


Lys Tyr Lys Lys Ile Phe Ser Glu Lys Tyr Asn Phe Asp Lys Asp Asn 
                325                 330                 335     


Thr Gly Asn Phe Val Val Asn Ile Asp Lys Phe Asn Ser Leu Tyr Ser 
            340                 345                 350         


Asp Leu Thr Asn Val Met Ser Glu Val Val Tyr Ser Ser Gln Tyr Asn 
        355                 360                 365             


Val Lys Asn Arg Thr His Tyr Phe Ser Arg His Tyr Leu Pro Val Phe 
    370                 375                 380                 


Ala Asn Ile Leu Asp Asp Asn Ile Tyr Thr Ile Arg Asp Gly Phe Asn 
385                 390                 395                 400 


Leu Thr Asn Lys Gly Phe Asn Ile Glu Asn Ser Gly Gln Asn Ile Glu 
                405                 410                 415     


Arg Asn Pro Ala Leu Gln Lys Leu Ser Ser Glu Ser Val Val Asp Leu 
            420                 425                 430         


Phe Thr Lys Val Cys Leu Arg Leu Thr Lys Asn Ser Arg Asp Asp Ser 
        435                 440                 445             


Thr Cys Ile Lys Val Lys Asn Asn Arg Leu Pro Tyr Val Ala Asp Lys 
    450                 455                 460                 


Asp Ser Ile Ser Gln Glu Ile Phe Glu Asn Lys Ile Ile Thr Asp Glu 
465                 470                 475                 480 


Thr Asn Val Gln Asn Tyr Ser Asp Lys Phe Ser Leu Asp Glu Ser Ile 
                485                 490                 495     


Leu Asp Gly Gln Val Pro Ile Asn Pro Glu Ile Val Asp Pro Leu Leu 
            500                 505                 510         


Pro Asn Val Asn Met Glu Pro Leu Asn Leu Pro Gly Glu Glu Ile Val 
        515                 520                 525             


Phe Tyr Asp Asp Ile Thr Lys Tyr Val Asp Tyr Leu Asn Ser Tyr Tyr 
    530                 535                 540                 


Tyr Leu Glu Ser Gln Lys Leu Ser Asn Asn Val Glu Asn Ile Thr Leu 
545                 550                 555                 560 


Thr Thr Ser Val Glu Glu Ala Leu Gly Tyr Ser Asn Lys Ile Tyr Thr 
                565                 570                 575     


Phe Leu Pro Ser Leu Ala Glu Lys Val Asn Lys Gly Val Gln Ala Gly 
            580                 585                 590         


Leu Phe Leu Asn Trp Ala Asn Glu Val Val Glu Asp Phe Thr Thr Asn 
        595                 600                 605             


Ile Met Lys Lys Asp Thr Leu Asp Lys Ile Ser Asp Val Ser Val Ile 
    610                 615                 620                 


Ile Pro Tyr Ile Gly Pro Ala Leu Asn Ile Gly Asn Ser Ala Leu Arg 
625                 630                 635                 640 


Gly Asn Phe Asn Gln Ala Phe Ala Thr Ala Gly Val Ala Phe Leu Leu 
                645                 650                 655     


Glu Gly Phe Pro Glu Phe Thr Ile Pro Ala Leu Gly Val Phe Thr Phe 
            660                 665                 670         


Tyr Ser Ser Ile Gln Glu Arg Glu Lys Ile Ile Lys Thr Ile Glu Asn 
        675                 680                 685             


Cys Leu Glu Gln Arg Val Lys Arg Trp Lys Asp Ser Tyr Gln Trp Met 
    690                 695                 700                 


Val Ser Asn Trp Leu Ser Arg Ile Thr Thr Gln Phe Asn His Ile Asn 
705                 710                 715                 720 


Tyr Gln Met Tyr Asp Ser Leu Ser Tyr Gln Ala Asp Ala Ile Lys Ala 
                725                 730                 735     


Lys Ile Asp Leu Glu Tyr Lys Lys Tyr Ser Gly Ser Asp Lys Glu Asn 
            740                 745                 750         


Ile Lys Ser Gln Val Glu Asn Leu Lys Asn Ser Leu Asp Val Lys Ile 
        755                 760                 765             


Ser Glu Ala Met Asn Asn Ile Asn Lys Phe Ile Arg Glu Cys Ser Val 
    770                 775                 780                 


Thr Tyr Leu Phe Lys Asn Met Leu Pro Lys Val Ile Asp Glu Leu Asn 
785                 790                 795                 800 


Lys Phe Asp Leu Arg Thr Lys Thr Glu Leu Ile Asn Leu Ile Asp Ser 
                805                 810                 815     


His Asn Ile Ile Leu Val Gly Glu Val Asp Arg Leu Lys Ala Lys Val 
            820                 825                 830         


Asn Glu Ser Phe Glu Asn Thr Met Pro Phe Asn Ile Phe Ser Tyr Thr 
        835                 840                 845             


Asn Asn Ser Leu Leu Lys Asp Ile Ile Asn Glu Tyr Phe Asn Ser Ile 
    850                 855                 860                 


Asn Asp Ser Lys Ile Leu Ser Leu Gln Asn Lys Lys Asn Ala Leu Val 
865                 870                 875                 880 


Asp Thr Ser Gly Tyr Asn Ala Glu Val Arg Val Gly Asp Asn Val Gln 
                885                 890                 895     


Leu Asn Thr Ile Tyr Thr Asn Asp Phe Lys Leu Ser Ser Ser Gly Asp 
            900                 905                 910         


Lys Ile Ile Val Asn Leu Asn Asn Asn Ile Leu Tyr Ser Ala Ile Tyr 
        915                 920                 925             


Glu Asn Ser Ser Val Ser Phe Trp Ile Lys Ile Ser Lys Asp Leu Thr 
    930                 935                 940                 


Asn Ser His Asn Glu Tyr Thr Ile Ile Asn Ser Ile Glu Gln Asn Ser 
945                 950                 955                 960 


Gly Trp Lys Leu Cys Ile Arg Asn Gly Asn Ile Glu Trp Ile Leu Gln 
                965                 970                 975     


Asp Val Asn Arg Lys Tyr Lys Ser Leu Ile Phe Asp Tyr Ser Glu Ser 
            980                 985                 990         


Leu Ser His Thr Gly Tyr Thr Asn  Lys Trp Phe Phe Val  Thr Ile Thr 
        995                 1000                 1005             


Asn Asn  Ile Met Gly Tyr Met  Lys Leu Tyr Ile Asn  Gly Glu Leu 
    1010                 1015                 1020             


Lys Gln  Ser Gln Lys Ile Glu  Asp Leu Asp Glu Val  Lys Leu Asp 
    1025                 1030                 1035             


Lys Thr  Ile Val Phe Gly Ile  Asp Glu Asn Ile Asp  Glu Asn Gln 
    1040                 1045                 1050             


Met Leu  Trp Ile Arg Asp Phe  Asn Ile Phe Ser Lys  Glu Leu Ser 
    1055                 1060                 1065             


Asn Glu  Asp Ile Asn Ile Val  Tyr Glu Gly Gln Ile  Leu Arg Asn 
    1070                 1075                 1080             


Val Ile  Lys Asp Tyr Trp Gly  Asn Pro Leu Lys Phe  Asp Thr Glu 
    1085                 1090                 1095             


Tyr Tyr  Ile Ile Asn Asp Asn  Tyr Ile Asp Arg Tyr  Ile Ala Pro 
    1100                 1105                 1110             


Glu Ser  Asn Val Leu Val Leu  Val Gln Tyr Pro Asp  Arg Ser Lys 
    1115                 1120                 1125             


Leu Tyr  Thr Gly Asn Pro Ile  Thr Ile Lys Ser Val  Ser Asp Lys 
    1130                 1135                 1140             


Asn Pro  Tyr Ser Arg Ile Leu  Asn Gly Asp Asn Ile  Ile Leu His 
    1145                 1150                 1155             


Met Leu  Tyr Asn Ser Arg Lys  Tyr Met Ile Ile Arg  Asp Thr Asp 
    1160                 1165                 1170             


Thr Ile  Tyr Ala Thr Gln Gly  Gly Glu Cys Ser Gln  Asn Cys Val 
    1175                 1180                 1185             


Tyr Ala  Leu Lys Leu Gln Ser  Asn Leu Gly Asn Tyr  Gly Ile Gly 
    1190                 1195                 1200             


Ile Phe  Ser Ile Lys Asn Ile  Val Ser Lys Asn Lys  Tyr Cys Ser 
    1205                 1210                 1215             


Gln Ile  Phe Ser Ser Phe Arg  Glu Asn Thr Met Leu  Leu Ala Asp 
    1220                 1225                 1230             


Ile Tyr  Lys Pro Trp Arg Phe  Ser Phe Lys Asn Ala  Tyr Thr Pro 
    1235                 1240                 1245             


Val Ala  Val Thr Asn Tyr Glu  Thr Lys Leu Leu Ser  Thr Ser Ser 
    1250                 1255                 1260             


Phe Trp  Lys Phe Ile Ser Arg  Asp Pro Gly Trp Val  Glu 
    1265                 1270                 1275     


<210>  55
<211>  1251
<212>  PRT
<213>  Clostridium botulinum

<400>  55

Met Pro Lys Ile Asn Ser Phe Asn Tyr Asn Asp Pro Val Asn Asp Arg 
1               5                   10                  15      


Thr Ile Leu Tyr Ile Lys Pro Gly Gly Cys Gln Glu Phe Tyr Lys Ser 
            20                  25                  30          


Phe Asn Ile Met Lys Asn Ile Trp Ile Ile Pro Glu Arg Asn Val Ile 
        35                  40                  45              


Gly Thr Thr Pro Gln Asp Phe His Pro Pro Thr Ser Leu Lys Asn Gly 
    50                  55                  60                  


Asp Ser Ser Tyr Tyr Asp Pro Asn Tyr Leu Gln Ser Asp Glu Glu Lys 
65                  70                  75                  80  


Asp Arg Phe Leu Lys Ile Val Thr Lys Ile Phe Asn Arg Ile Asn Asn 
                85                  90                  95      


Asn Leu Ser Gly Gly Ile Leu Leu Glu Glu Leu Ser Lys Ala Asn Pro 
            100                 105                 110         


Tyr Leu Gly Asn Asp Asn Thr Pro Asp Asn Gln Phe His Ile Gly Asp 
        115                 120                 125             


Ala Ser Ala Val Glu Ile Lys Phe Ser Asn Gly Ser Gln Asp Ile Leu 
    130                 135                 140                 


Leu Pro Asn Val Ile Ile Met Gly Ala Glu Pro Asp Leu Phe Glu Thr 
145                 150                 155                 160 


Asn Ser Ser Asn Ile Ser Leu Arg Asn Asn Tyr Met Pro Ser Asn His 
                165                 170                 175     


Arg Phe Gly Ser Ile Ala Ile Val Thr Phe Ser Pro Glu Tyr Ser Phe 
            180                 185                 190         


Arg Phe Asn Asp Asn Cys Met Asn Glu Phe Ile Gln Asp Pro Ala Leu 
        195                 200                 205             


Thr Leu Met His Glu Leu Ile His Ser Leu His Gly Leu Tyr Gly Ala 
    210                 215                 220                 


Lys Gly Ile Thr Thr Lys Tyr Thr Ile Thr Gln Lys Gln Asn Pro Leu 
225                 230                 235                 240 


Ile Thr Asn Ile Arg Gly Thr Asn Ile Glu Glu Phe Leu Thr Phe Gly 
                245                 250                 255     


Gly Thr Asp Leu Asn Ile Ile Thr Ser Ala Gln Ser Asn Asp Ile Tyr 
            260                 265                 270         


Thr Asn Leu Leu Ala Asp Tyr Lys Lys Ile Ala Ser Lys Leu Ser Lys 
        275                 280                 285             


Val Gln Val Ser Asn Pro Leu Leu Asn Pro Tyr Lys Asp Val Phe Glu 
    290                 295                 300                 


Ala Lys Tyr Gly Leu Asp Lys Asp Ala Ser Gly Ile Tyr Ser Val Asn 
305                 310                 315                 320 


Ile Asn Lys Phe Asn Asp Ile Phe Lys Lys Leu Tyr Ser Phe Thr Glu 
                325                 330                 335     


Phe Asp Leu Arg Thr Lys Phe Gln Val Lys Cys Arg Gln Thr Tyr Ile 
            340                 345                 350         


Gly Gln Tyr Lys Tyr Phe Lys Leu Ser Asn Leu Leu Asn Asp Ser Ile 
        355                 360                 365             


Tyr Asn Ile Ser Glu Gly Tyr Asn Ile Asn Asn Leu Lys Val Asn Phe 
    370                 375                 380                 


Arg Gly Gln Asn Ala Asn Leu Asn Pro Arg Ile Ile Thr Pro Ile Thr 
385                 390                 395                 400 


Gly Arg Gly Leu Val Lys Lys Ile Ile Arg Phe Cys Lys Asn Ile Val 
                405                 410                 415     


Ser Val Lys Gly Ile Arg Lys Ser Ile Cys Ile Glu Ile Asn Asn Gly 
            420                 425                 430         


Glu Leu Phe Phe Val Ala Ser Glu Asn Ser Tyr Asn Asp Asp Asn Ile 
        435                 440                 445             


Asn Thr Pro Lys Glu Ile Asp Asp Thr Val Thr Ser Asn Asn Asn Tyr 
    450                 455                 460                 


Glu Asn Asp Leu Asp Gln Val Ile Leu Asn Phe Asn Ser Glu Ser Ala 
465                 470                 475                 480 


Pro Gly Leu Ser Asp Glu Lys Leu Asn Leu Thr Ile Gln Asn Asp Ala 
                485                 490                 495     


Tyr Ile Pro Lys Tyr Asp Ser Asn Gly Thr Ser Asp Ile Glu Gln His 
            500                 505                 510         


Asp Val Asn Glu Leu Asn Val Phe Phe Tyr Leu Asp Ala Gln Lys Val 
        515                 520                 525             


Pro Glu Gly Glu Asn Asn Val Asn Leu Thr Ser Ser Ile Asp Thr Ala 
    530                 535                 540                 


Leu Leu Glu Gln Pro Lys Ile Tyr Thr Phe Phe Ser Ser Glu Phe Ile 
545                 550                 555                 560 


Asn Asn Val Asn Lys Pro Val Gln Ala Ala Leu Phe Val Ser Trp Ile 
                565                 570                 575     


Gln Gln Val Leu Val Asp Phe Thr Thr Glu Ala Asn Gln Lys Ser Thr 
            580                 585                 590         


Val Asp Lys Ile Ala Asp Ile Ser Ile Val Val Pro Tyr Ile Gly Leu 
        595                 600                 605             


Ala Leu Asn Ile Gly Asn Glu Ala Gln Lys Gly Asn Phe Lys Asp Ala 
    610                 615                 620                 


Leu Glu Leu Leu Gly Ala Gly Ile Leu Leu Glu Phe Glu Pro Glu Leu 
625                 630                 635                 640 


Leu Ile Pro Thr Ile Leu Val Phe Thr Ile Lys Ser Phe Leu Gly Ser 
                645                 650                 655     


Ser Asp Asn Lys Asn Lys Val Ile Lys Ala Ile Asn Asn Ala Leu Lys 
            660                 665                 670         


Glu Arg Asp Glu Lys Trp Lys Glu Val Tyr Ser Phe Ile Val Ser Asn 
        675                 680                 685             


Trp Met Thr Lys Ile Asn Thr Gln Phe Asn Lys Arg Lys Glu Gln Met 
    690                 695                 700                 


Tyr Gln Ala Leu Gln Asn Gln Val Asn Ala Ile Lys Thr Ile Ile Glu 
705                 710                 715                 720 


Ser Lys Tyr Asn Ser Tyr Thr Leu Glu Glu Lys Asn Glu Leu Thr Asn 
                725                 730                 735     


Lys Tyr Asp Ile Lys Gln Ile Glu Asn Glu Leu Asn Gln Lys Val Ser 
            740                 745                 750         


Ile Ala Met Asn Asn Ile Asp Arg Phe Leu Thr Glu Ser Ser Ile Ser 
        755                 760                 765             


Tyr Leu Met Lys Ile Ile Asn Glu Val Lys Ile Asn Lys Leu Arg Glu 
    770                 775                 780                 


Tyr Asp Glu Asn Val Lys Thr Tyr Leu Leu Asn Tyr Ile Ile Gln His 
785                 790                 795                 800 


Gly Ser Ile Leu Gly Glu Ser Gln Gln Glu Leu Asn Ser Met Val Thr 
                805                 810                 815     


Asp Thr Leu Asn Asn Ser Ile Pro Phe Lys Leu Ser Ser Tyr Thr Asp 
            820                 825                 830         


Asp Lys Ile Leu Ile Ser Tyr Phe Asn Lys Phe Phe Lys Arg Ile Lys 
        835                 840                 845             


Ser Ser Ser Val Leu Asn Met Arg Tyr Lys Asn Asp Lys Tyr Val Asp 
    850                 855                 860                 


Thr Ser Gly Tyr Asp Ser Asn Ile Asn Ile Asn Gly Asp Val Tyr Lys 
865                 870                 875                 880 


Tyr Pro Thr Asn Lys Asn Gln Phe Gly Ile Tyr Asn Asp Lys Leu Ser 
                885                 890                 895     


Glu Val Asn Ile Ser Gln Asn Asp Tyr Ile Ile Tyr Asp Asn Lys Tyr 
            900                 905                 910         


Lys Asn Phe Ser Ile Ser Phe Trp Val Arg Ile Pro Asn Tyr Asp Asn 
        915                 920                 925             


Lys Ile Val Asn Val Asn Asn Glu Tyr Thr Ile Ile Asn Cys Met Arg 
    930                 935                 940                 


Asp Asn Asn Ser Gly Trp Lys Val Ser Leu Asn His Asn Glu Ile Ile 
945                 950                 955                 960 


Trp Thr Phe Glu Asp Asn Arg Gly Ile Asn Gln Lys Leu Ala Phe Asn 
                965                 970                 975     


Tyr Gly Asn Ala Asn Gly Ile Ser Asp Tyr Ile Asn Lys Trp Ile Phe 
            980                 985                 990         


Val Thr Ile Thr Asn Asp Arg Leu  Gly Asp Ser Lys Leu  Tyr Ile Asn 
        995                 1000                 1005             


Gly Asn  Leu Ile Asp Gln Lys  Ser Ile Leu Asn Leu  Gly Asn Ile 
    1010                 1015                 1020             


His Val  Ser Asp Asn Ile Leu  Phe Lys Ile Val Asn  Cys Ser Tyr 
    1025                 1030                 1035             


Thr Arg  Tyr Ile Gly Ile Arg  Tyr Phe Asn Ile Phe  Asp Lys Glu 
    1040                 1045                 1050             


Leu Asp  Glu Thr Glu Ile Gln  Thr Leu Tyr Ser Asn  Glu Pro Asn 
    1055                 1060                 1065             


Thr Asn  Ile Leu Lys Asp Phe  Trp Gly Asn Tyr Leu  Leu Tyr Asp 
    1070                 1075                 1080             


Lys Glu  Tyr Tyr Leu Leu Asn  Val Leu Lys Pro Asn  Asn Phe Ile 
    1085                 1090                 1095             


Asp Arg  Arg Lys Asp Ser Thr  Leu Ser Ile Asn Asn  Ile Arg Ser 
    1100                 1105                 1110             


Thr Ile  Leu Leu Ala Asn Arg  Leu Tyr Ser Gly Ile  Lys Val Lys 
    1115                 1120                 1125             


Ile Gln  Arg Val Asn Asn Ser  Ser Thr Asn Asp Asn  Leu Val Arg 
    1130                 1135                 1140             


Lys Asn  Asp Gln Val Tyr Ile  Asn Phe Val Ala Ser  Lys Thr His 
    1145                 1150                 1155             


Leu Phe  Pro Leu Tyr Ala Asp  Thr Ala Thr Thr Asn  Lys Glu Lys 
    1160                 1165                 1170             


Thr Ile  Lys Ile Ser Ser Ser  Gly Asn Arg Phe Asn  Gln Val Val 
    1175                 1180                 1185             


Val Met  Asn Ser Val Gly Asn  Cys Thr Met Asn Phe  Lys Asn Asn 
    1190                 1195                 1200             


Asn Gly  Asn Asn Ile Gly Leu  Leu Gly Phe Lys Ala  Asp Thr Val 
    1205                 1210                 1215             


Val Ala  Ser Thr Trp Tyr Tyr  Thr His Met Arg Asp  His Thr Asn 
    1220                 1225                 1230             


Ser Asn  Gly Cys Phe Trp Asn  Phe Ile Ser Glu Glu  His Gly Trp 
    1235                 1240                 1245             


Gln Glu  Lys 
    1250     


<210>  56
<211>  1278
<212>  PRT
<213>  Clostridium botulinum

<400>  56

Met Pro Val Val Ile Asn Ser Phe Asn Tyr Asn Asp Pro Val Asn Asp 
1               5                   10                  15      


Asp Thr Ile Leu Tyr Met Gln Ile Pro Tyr Glu Glu Lys Ser Lys Lys 
            20                  25                  30          


Tyr Tyr Lys Ala Phe Glu Ile Met Arg Asn Val Trp Ile Ile Pro Glu 
        35                  40                  45              


Arg Asn Thr Ile Gly Thr Asp Pro Ser Asp Phe Asp Pro Pro Ala Ser 
    50                  55                  60                  


Leu Glu Asn Gly Ser Ser Ala Tyr Tyr Asp Pro Asn Tyr Leu Thr Thr 
65                  70                  75                  80  


Asp Ala Glu Lys Asp Arg Tyr Leu Lys Thr Thr Ile Lys Leu Phe Lys 
                85                  90                  95      


Arg Ile Asn Ser Asn Pro Ala Gly Glu Val Leu Leu Gln Glu Ile Ser 
            100                 105                 110         


Tyr Ala Lys Pro Tyr Leu Gly Asn Glu His Thr Pro Ile Asn Glu Phe 
        115                 120                 125             


His Pro Val Thr Arg Thr Thr Ser Val Asn Ile Lys Ser Ser Thr Asn 
    130                 135                 140                 


Val Lys Ser Ser Ile Ile Leu Asn Leu Leu Val Leu Gly Ala Gly Pro 
145                 150                 155                 160 


Asp Ile Phe Glu Asn Ser Ser Tyr Pro Val Arg Lys Leu Met Asp Ser 
                165                 170                 175     


Gly Gly Val Tyr Asp Pro Ser Asn Asp Gly Phe Gly Ser Ile Asn Ile 
            180                 185                 190         


Val Thr Phe Ser Pro Glu Tyr Glu Tyr Thr Phe Asn Asp Ile Ser Gly 
        195                 200                 205             


Gly Tyr Asn Ser Ser Thr Glu Ser Phe Ile Ala Asp Pro Ala Ile Ser 
    210                 215                 220                 


Leu Ala His Glu Leu Ile His Ala Leu His Gly Leu Tyr Gly Ala Arg 
225                 230                 235                 240 


Gly Val Thr Tyr Lys Glu Thr Ile Lys Val Lys Gln Ala Pro Leu Met 
                245                 250                 255     


Ile Ala Glu Lys Pro Ile Arg Leu Glu Glu Phe Leu Thr Phe Gly Gly 
            260                 265                 270         


Gln Asp Leu Asn Ile Ile Thr Ser Ala Met Lys Glu Lys Ile Tyr Asn 
        275                 280                 285             


Asn Leu Leu Ala Asn Tyr Glu Lys Ile Ala Thr Arg Leu Ser Arg Val 
    290                 295                 300                 


Asn Ser Ala Pro Pro Glu Tyr Asp Ile Asn Glu Tyr Lys Asp Tyr Phe 
305                 310                 315                 320 


Gln Trp Lys Tyr Gly Leu Asp Lys Asn Ala Asp Gly Ser Tyr Thr Val 
                325                 330                 335     


Asn Glu Asn Lys Phe Asn Glu Ile Tyr Lys Lys Leu Tyr Ser Phe Thr 
            340                 345                 350         


Glu Ile Asp Leu Ala Asn Lys Phe Lys Val Lys Cys Arg Asn Thr Tyr 
        355                 360                 365             


Phe Ile Lys Tyr Gly Phe Leu Lys Val Pro Asn Leu Leu Asp Asp Asp 
    370                 375                 380                 


Ile Tyr Thr Val Ser Glu Gly Phe Asn Ile Gly Asn Leu Ala Val Asn 
385                 390                 395                 400 


Asn Arg Gly Gln Asn Ile Lys Leu Asn Pro Lys Ile Ile Asp Ser Ile 
                405                 410                 415     


Pro Asp Lys Gly Leu Val Glu Lys Ile Val Lys Phe Cys Lys Ser Val 
            420                 425                 430         


Ile Pro Arg Lys Gly Thr Lys Ala Pro Pro Arg Leu Cys Ile Arg Val 
        435                 440                 445             


Asn Asn Arg Glu Leu Phe Phe Val Ala Ser Glu Ser Ser Tyr Asn Glu 
    450                 455                 460                 


Asn Asp Ile Asn Thr Pro Lys Glu Ile Asp Asp Thr Thr Asn Leu Asn 
465                 470                 475                 480 


Asn Asn Tyr Arg Asn Asn Leu Asp Glu Val Ile Leu Asp Tyr Asn Ser 
                485                 490                 495     


Glu Thr Ile Pro Gln Ile Ser Asn Gln Thr Leu Asn Thr Leu Val Gln 
            500                 505                 510         


Asp Asp Ser Tyr Val Pro Arg Tyr Asp Ser Asn Gly Thr Ser Glu Ile 
        515                 520                 525             


Glu Glu His Asn Val Val Asp Leu Asn Val Phe Phe Tyr Leu His Ala 
    530                 535                 540                 


Gln Lys Val Pro Glu Gly Glu Thr Asn Ile Ser Leu Thr Ser Ser Ile 
545                 550                 555                 560 


Asp Thr Ala Leu Ser Glu Glu Ser Gln Val Tyr Thr Phe Phe Ser Ser 
                565                 570                 575     


Glu Phe Ile Asn Thr Ile Asn Lys Pro Val His Ala Ala Leu Phe Ile 
            580                 585                 590         


Ser Trp Ile Asn Gln Val Ile Arg Asp Phe Thr Thr Glu Ala Thr Gln 
        595                 600                 605             


Lys Ser Thr Phe Asp Lys Ile Ala Asp Ile Ser Leu Val Val Pro Tyr 
    610                 615                 620                 


Val Gly Leu Ala Leu Asn Ile Gly Asn Glu Val Gln Lys Glu Asn Phe 
625                 630                 635                 640 


Lys Glu Ala Phe Glu Leu Leu Gly Ala Gly Ile Leu Leu Glu Phe Val 
                645                 650                 655     


Pro Glu Leu Leu Ile Pro Thr Ile Leu Val Phe Thr Ile Lys Ser Phe 
            660                 665                 670         


Ile Gly Ser Ser Glu Asn Lys Asn Lys Ile Ile Lys Ala Ile Asn Asn 
        675                 680                 685             


Ser Leu Met Glu Arg Glu Thr Lys Trp Lys Glu Ile Tyr Ser Trp Ile 
    690                 695                 700                 


Val Ser Asn Trp Leu Thr Arg Ile Asn Thr Gln Phe Asn Lys Arg Lys 
705                 710                 715                 720 


Glu Gln Met Tyr Gln Ala Leu Gln Asn Gln Val Asp Ala Ile Lys Thr 
                725                 730                 735     


Val Ile Glu Tyr Lys Tyr Asn Asn Tyr Thr Ser Asp Glu Arg Asn Arg 
            740                 745                 750         


Leu Glu Ser Glu Tyr Asn Ile Asn Asn Ile Arg Glu Glu Leu Asn Lys 
        755                 760                 765             


Lys Val Ser Leu Ala Met Glu Asn Ile Glu Arg Phe Ile Thr Glu Ser 
    770                 775                 780                 


Ser Ile Phe Tyr Leu Met Lys Leu Ile Asn Glu Ala Lys Val Ser Lys 
785                 790                 795                 800 


Leu Arg Glu Tyr Asp Glu Gly Val Lys Glu Tyr Leu Leu Asp Tyr Ile 
                805                 810                 815     


Ser Glu His Arg Ser Ile Leu Gly Asn Ser Val Gln Glu Leu Asn Asp 
            820                 825                 830         


Leu Val Thr Ser Thr Leu Asn Asn Ser Ile Pro Phe Glu Leu Ser Ser 
        835                 840                 845             


Tyr Thr Asn Asp Lys Ile Leu Ile Leu Tyr Phe Asn Lys Leu Tyr Lys 
    850                 855                 860                 


Lys Ile Lys Asp Asn Ser Ile Leu Asp Met Arg Tyr Glu Asn Asn Lys 
865                 870                 875                 880 


Phe Ile Asp Ile Ser Gly Tyr Gly Ser Asn Ile Ser Ile Asn Gly Asp 
                885                 890                 895     


Val Tyr Ile Tyr Ser Thr Asn Arg Asn Gln Phe Gly Ile Tyr Ser Ser 
            900                 905                 910         


Lys Pro Ser Glu Val Asn Ile Ala Gln Asn Asn Asp Ile Ile Tyr Asn 
        915                 920                 925             


Gly Arg Tyr Gln Asn Phe Ser Ile Ser Phe Trp Val Arg Ile Pro Lys 
    930                 935                 940                 


Tyr Phe Asn Lys Val Asn Leu Asn Asn Glu Tyr Thr Ile Ile Asp Cys 
945                 950                 955                 960 


Ile Arg Asn Asn Asn Ser Gly Trp Lys Ile Ser Leu Asn Tyr Asn Lys 
                965                 970                 975     


Ile Ile Trp Thr Leu Gln Asp Thr Ala Gly Asn Asn Gln Lys Leu Val 
            980                 985                 990         


Phe Asn Tyr Thr Gln Met Ile Ser  Ile Ser Asp Tyr Ile  Asn Lys Trp 
        995                 1000                 1005             


Ile Phe  Val Thr Ile Thr Asn  Asn Arg Leu Gly Asn  Ser Arg Ile 
    1010                 1015                 1020             


Tyr Ile  Asn Gly Asn Leu Ile  Asp Glu Lys Ser Ile  Ser Asn Leu 
    1025                 1030                 1035             


Gly Asp  Ile His Val Ser Asp  Asn Ile Leu Phe Lys  Ile Val Gly 
    1040                 1045                 1050             


Cys Asn  Asp Thr Arg Tyr Val  Gly Ile Arg Tyr Phe  Lys Val Phe 
    1055                 1060                 1065             


Asp Thr  Glu Leu Gly Lys Thr  Glu Ile Glu Thr Leu  Tyr Ser Asp 
    1070                 1075                 1080             


Glu Pro  Asp Pro Ser Ile Leu  Lys Asp Phe Trp Gly  Asn Tyr Leu 
    1085                 1090                 1095             


Leu Tyr  Asn Lys Arg Tyr Tyr  Leu Leu Asn Leu Leu  Arg Thr Asp 
    1100                 1105                 1110             


Lys Ser  Ile Thr Gln Asn Ser  Asn Phe Leu Asn Ile  Asn Gln Gln 
    1115                 1120                 1125             


Arg Gly  Val Tyr Gln Lys Pro  Asn Ile Phe Ser Asn  Thr Arg Leu 
    1130                 1135                 1140             


Tyr Thr  Gly Val Glu Val Ile  Ile Arg Lys Asn Gly  Ser Thr Asp 
    1145                 1150                 1155             


Ile Ser  Asn Thr Asp Asn Phe  Val Arg Lys Asn Asp  Leu Ala Tyr 
    1160                 1165                 1170             


Ile Asn  Val Val Asp Arg Asp  Val Glu Tyr Arg Leu  Tyr Ala Asp 
    1175                 1180                 1185             


Ile Ser  Ile Ala Lys Pro Glu  Lys Ile Ile Lys Leu  Ile Arg Thr 
    1190                 1195                 1200             


Ser Asn  Ser Asn Asn Ser Leu  Gly Gln Ile Ile Val  Met Asp Ser 
    1205                 1210                 1215             


Ile Gly  Asn Asn Cys Thr Met  Asn Phe Gln Asn Asn  Asn Gly Gly 
    1220                 1225                 1230             


Asn Ile  Gly Leu Leu Gly Phe  His Ser Asn Asn Leu  Val Ala Ser 
    1235                 1240                 1245             


Ser Trp  Tyr Tyr Asn Asn Ile  Arg Lys Asn Thr Ser  Ser Asn Gly 
    1250                 1255                 1260             


Cys Phe  Trp Ser Phe Ile Ser  Lys Glu His Gly Trp  Gln Glu Asn 
    1265                 1270                 1275             


<210>  57
<211>  1297
<212>  PRT
<213>  Clostridium botulinum


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Xaa can be any naturally occurring amino acid

<400>  57

Met Pro Val Asn Ile Lys Xaa Phe Asn Tyr Asn Asp Pro Ile Asn Asn 
1               5                   10                  15      


Asp Asp Ile Ile Met Met Glu Pro Phe Asn Asp Pro Gly Pro Gly Thr 
            20                  25                  30          


Tyr Tyr Lys Ala Phe Arg Ile Ile Asp Arg Ile Trp Ile Val Pro Glu 
        35                  40                  45              


Arg Phe Thr Tyr Gly Phe Gln Pro Asp Gln Phe Asn Ala Ser Thr Gly 
    50                  55                  60                  


Val Phe Ser Lys Asp Val Tyr Glu Tyr Tyr Asp Pro Thr Tyr Leu Lys 
65                  70                  75                  80  


Thr Asp Ala Glu Lys Asp Lys Phe Leu Lys Thr Met Ile Lys Leu Phe 
                85                  90                  95      


Asn Arg Ile Asn Ser Lys Pro Ser Gly Gln Arg Leu Leu Asp Met Ile 
            100                 105                 110         


Val Asp Ala Ile Pro Tyr Leu Gly Asn Ala Ser Thr Pro Pro Asp Lys 
        115                 120                 125             


Phe Ala Ala Asn Val Ala Asn Val Ser Ile Asn Lys Lys Ile Ile Gln 
    130                 135                 140                 


Pro Gly Ala Glu Asp Gln Ile Lys Gly Leu Met Thr Asn Leu Ile Ile 
145                 150                 155                 160 


Phe Gly Pro Gly Pro Val Leu Ser Asp Asn Phe Thr Asp Ser Met Ile 
                165                 170                 175     


Met Asn Gly His Ser Pro Ile Ser Glu Gly Phe Gly Ala Arg Met Met 
            180                 185                 190         


Ile Arg Phe Cys Pro Ser Cys Leu Asn Val Phe Asn Asn Val Gln Glu 
        195                 200                 205             


Asn Lys Asp Thr Ser Ile Phe Ser Arg Arg Ala Tyr Phe Ala Asp Pro 
    210                 215                 220                 


Ala Leu Thr Leu Met His Glu Leu Ile His Val Leu His Gly Leu Tyr 
225                 230                 235                 240 


Gly Ile Lys Ile Ser Asn Leu Pro Ile Thr Pro Asn Thr Lys Glu Phe 
                245                 250                 255     


Phe Met Gln His Ser Asp Pro Val Gln Ala Glu Glu Leu Tyr Thr Phe 
            260                 265                 270         


Gly Gly His Asp Pro Ser Val Ile Ser Pro Ser Thr Asp Met Asn Ile 
        275                 280                 285             


Tyr Asn Lys Ala Leu Gln Asn Phe Gln Asp Ile Ala Asn Arg Leu Asn 
    290                 295                 300                 


Ile Val Ser Ser Ala Gln Gly Ser Gly Ile Asp Ile Ser Leu Tyr Lys 
305                 310                 315                 320 


Gln Ile Tyr Lys Asn Lys Tyr Asp Phe Val Glu Asp Pro Asn Gly Lys 
                325                 330                 335     


Tyr Ser Val Asp Lys Asp Lys Phe Asp Lys Leu Tyr Lys Ala Leu Met 
            340                 345                 350         


Phe Gly Phe Thr Glu Thr Asn Leu Ala Gly Glu Tyr Gly Ile Lys Thr 
        355                 360                 365             


Arg Tyr Ser Tyr Phe Ser Glu Tyr Leu Pro Pro Ile Lys Thr Glu Lys 
    370                 375                 380                 


Leu Leu Asp Asn Thr Ile Tyr Thr Gln Asn Glu Gly Phe Asn Ile Ala 
385                 390                 395                 400 


Ser Lys Asn Leu Lys Thr Glu Phe Asn Gly Gln Asn Lys Ala Val Asn 
                405                 410                 415     


Lys Glu Ala Tyr Glu Glu Ile Ser Leu Glu His Leu Val Ile Tyr Arg 
            420                 425                 430         


Ile Ala Met Cys Lys Pro Val Met Tyr Lys Asn Thr Gly Lys Ser Glu 
        435                 440                 445             


Gln Cys Ile Ile Val Asn Asn Glu Asp Leu Phe Phe Ile Ala Asn Lys 
    450                 455                 460                 


Asp Ser Phe Ser Lys Asp Leu Ala Lys Ala Glu Thr Ile Ala Tyr Asn 
465                 470                 475                 480 


Thr Gln Asn Asn Thr Ile Glu Asn Asn Phe Ser Ile Asp Gln Leu Ile 
                485                 490                 495     


Leu Asp Asn Asp Leu Ser Ser Gly Ile Asp Leu Pro Asn Glu Asn Thr 
            500                 505                 510         


Glu Pro Phe Thr Asn Phe Asp Asp Ile Asp Ile Pro Val Tyr Ile Lys 
        515                 520                 525             


Gln Ser Ala Leu Lys Lys Ile Phe Val Asp Gly Asp Ser Leu Phe Glu 
    530                 535                 540                 


Tyr Leu His Ala Gln Thr Phe Pro Ser Asn Ile Glu Asn Leu Gln Leu 
545                 550                 555                 560 


Thr Asn Ser Leu Asn Asp Ala Leu Arg Asn Asn Asn Lys Val Tyr Thr 
                565                 570                 575     


Phe Phe Ser Thr Asn Leu Val Glu Lys Ala Asn Thr Val Val Gly Ala 
            580                 585                 590         


Ser Leu Phe Val Asn Trp Val Lys Gly Val Ile Asp Asp Phe Thr Ser 
        595                 600                 605             


Glu Ser Thr Gln Lys Ser Thr Ile Asp Lys Val Ser Asp Val Ser Ile 
    610                 615                 620                 


Ile Ile Pro Tyr Ile Gly Pro Ala Leu Asn Val Gly Asn Glu Thr Ala 
625                 630                 635                 640 


Lys Glu Asn Phe Lys Asn Ala Phe Glu Ile Gly Gly Ala Ala Ile Leu 
                645                 650                 655     


Met Glu Phe Ile Pro Glu Leu Ile Val Pro Ile Val Gly Phe Phe Thr 
            660                 665                 670         


Leu Glu Ser Tyr Val Gly Asn Lys Gly His Ile Ile Met Thr Ile Ser 
        675                 680                 685             


Asn Ala Leu Lys Lys Arg Asp Gln Lys Trp Thr Asp Met Tyr Gly Leu 
    690                 695                 700                 


Ile Val Ser Gln Trp Leu Ser Thr Val Asn Thr Gln Phe Tyr Thr Ile 
705                 710                 715                 720 


Lys Glu Arg Met Tyr Asn Ala Leu Asn Asn Gln Ser Gln Ala Ile Glu 
                725                 730                 735     


Lys Ile Ile Glu Asp Gln Tyr Asn Arg Tyr Ser Glu Glu Asp Lys Met 
            740                 745                 750         


Asn Ile Asn Ile Asp Phe Asn Asp Ile Asp Phe Lys Leu Asn Gln Ser 
        755                 760                 765             


Ile Asn Leu Ala Ile Asn Asn Ile Asp Asp Phe Ile Asn Gln Cys Ser 
    770                 775                 780                 


Ile Ser Tyr Leu Met Asn Arg Met Ile Pro Leu Ala Val Lys Lys Leu 
785                 790                 795                 800 


Lys Asp Phe Asp Asp Asn Leu Lys Arg Asp Leu Leu Glu Tyr Ile Asp 
                805                 810                 815     


Thr Asn Glu Leu Tyr Leu Leu Asp Glu Val Asn Ile Leu Lys Ser Lys 
            820                 825                 830         


Val Asn Arg His Leu Lys Asp Ser Ile Pro Phe Asp Leu Ser Leu Tyr 
        835                 840                 845             


Thr Lys Asp Thr Ile Leu Ile Gln Val Phe Asn Asn Tyr Ile Ser Asn 
    850                 855                 860                 


Ile Ser Ser Asn Ala Ile Leu Ser Leu Ser Tyr Arg Gly Gly Arg Leu 
865                 870                 875                 880 


Ile Asp Ser Ser Gly Tyr Gly Ala Thr Met Asn Val Gly Ser Asp Val 
                885                 890                 895     


Ile Phe Asn Asp Ile Gly Asn Gly Gln Phe Lys Leu Asn Asn Ser Glu 
            900                 905                 910         


Asn Ser Asn Ile Thr Ala His Gln Ser Lys Phe Val Val Tyr Asp Ser 
        915                 920                 925             


Met Phe Asp Asn Phe Ser Ile Asn Phe Trp Val Arg Thr Pro Lys Tyr 
    930                 935                 940                 


Asn Asn Asn Asp Ile Gln Thr Tyr Leu Gln Asn Glu Tyr Thr Ile Ile 
945                 950                 955                 960 


Ser Cys Ile Lys Asn Asp Ser Gly Trp Lys Val Ser Ile Lys Gly Asn 
                965                 970                 975     


Arg Ile Ile Trp Thr Leu Ile Asp Val Asn Ala Lys Ser Lys Ser Ile 
            980                 985                 990         


Phe Phe Glu Tyr Ser Ile Lys Asp  Asn Ile Ser Asp Tyr  Ile Asn Lys 
        995                 1000                 1005             


Trp Phe  Ser Ile Thr Ile Thr  Asn Asp Arg Leu Gly  Asn Ala Asn 
    1010                 1015                 1020             


Ile Tyr  Ile Asn Gly Ser Leu  Lys Lys Ser Glu Lys  Ile Leu Asn 
    1025                 1030                 1035             


Leu Asp  Arg Ile Asn Ser Ser  Asn Asp Ile Asp Phe  Lys Leu Ile 
    1040                 1045                 1050             


Asn Cys  Thr Asp Thr Thr Lys  Phe Val Trp Ile Lys  Asp Phe Asn 
    1055                 1060                 1065             


Ile Phe  Gly Arg Glu Leu Asn  Ala Thr Glu Val Ser  Ser Leu Tyr 
    1070                 1075                 1080             


Trp Ile  Gln Ser Ser Thr Asn  Thr Leu Lys Asp Phe  Trp Gly Asn 
    1085                 1090                 1095             


Pro Leu  Arg Tyr Asp Thr Gln  Tyr Tyr Leu Phe Asn  Gln Gly Met 
    1100                 1105                 1110             


Gln Asn  Ile Tyr Ile Lys Tyr  Phe Ser Lys Ala Ser  Met Gly Glu 
    1115                 1120                 1125             


Thr Ala  Pro Arg Thr Asn Phe  Asn Asn Ala Ala Ile  Asn Tyr Gln 
    1130                 1135                 1140             


Asn Leu  Tyr Leu Gly Leu Arg  Phe Ile Ile Lys Lys  Ala Ser Asn 
    1145                 1150                 1155             


Ser Arg  Asn Ile Asn Asn Asp  Asn Ile Val Arg Glu  Gly Asp Tyr 
    1160                 1165                 1170             


Ile Tyr  Leu Asn Ile Asp Asn  Ile Ser Asp Glu Ser  Tyr Arg Val 
    1175                 1180                 1185             


Tyr Val  Leu Val Asn Ser Lys  Glu Ile Gln Thr Gln  Leu Phe Leu 
    1190                 1195                 1200             


Ala Pro  Ile Asn Asp Asp Pro  Thr Phe Tyr Asp Val  Leu Gln Ile 
    1205                 1210                 1215             


Lys Lys  Tyr Tyr Glu Lys Thr  Thr Tyr Asn Cys Gln  Ile Leu Cys 
    1220                 1225                 1230             


Glu Lys  Asp Thr Lys Thr Phe  Gly Leu Phe Gly Ile  Gly Lys Phe 
    1235                 1240                 1245             


Val Lys  Asp Tyr Gly Tyr Val  Trp Asp Thr Tyr Asp  Asn Tyr Phe 
    1250                 1255                 1260             


Cys Ile  Ser Gln Trp Tyr Leu  Arg Arg Ile Ser Glu  Asn Ile Asn 
    1265                 1270                 1275             


Lys Leu  Arg Leu Gly Cys Asn  Trp Gln Phe Ile Pro  Val Asp Glu 
    1280                 1285                 1290             


Gly Trp  Thr Glu 
    1295         


<210>  58
<211>  1315
<212>  PRT
<213>  Clostridium tetani

<400>  58

Met Pro Ile Thr Ile Asn Asn Phe Arg Tyr Ser Asp Pro Val Asn Asn 
1               5                   10                  15      


Asp Thr Ile Ile Met Met Glu Pro Pro Tyr Cys Lys Gly Leu Asp Ile 
            20                  25                  30          


Tyr Tyr Lys Ala Phe Lys Ile Thr Asp Arg Ile Trp Ile Val Pro Glu 
        35                  40                  45              


Arg Tyr Glu Phe Gly Thr Lys Pro Glu Asp Phe Asn Pro Pro Ser Ser 
    50                  55                  60                  


Leu Ile Glu Gly Ala Ser Glu Tyr Tyr Asp Pro Asn Tyr Leu Arg Thr 
65                  70                  75                  80  


Asp Ser Asp Lys Asp Arg Phe Leu Gln Thr Met Val Lys Leu Phe Asn 
                85                  90                  95      


Arg Ile Lys Asn Asn Val Ala Gly Glu Ala Leu Leu Asp Lys Ile Ile 
            100                 105                 110         


Asn Ala Ile Pro Tyr Leu Gly Asn Ser Tyr Ser Leu Leu Asp Lys Phe 
        115                 120                 125             


Asp Thr Asn Ser Asn Ser Val Ser Phe Asn Leu Leu Glu Gln Asp Pro 
    130                 135                 140                 


Ser Gly Ala Thr Thr Lys Ser Ala Met Leu Thr Asn Leu Ile Ile Phe 
145                 150                 155                 160 


Gly Pro Gly Pro Val Leu Asn Lys Asn Glu Val Arg Gly Ile Val Leu 
                165                 170                 175     


Arg Val Asp Asn Lys Asn Tyr Phe Pro Cys Arg Asp Gly Phe Gly Ser 
            180                 185                 190         


Ile Met Gln Met Ala Phe Cys Pro Glu Tyr Val Pro Thr Phe Asp Asn 
        195                 200                 205             


Val Ile Glu Asn Ile Thr Ser Leu Thr Ile Gly Lys Ser Lys Tyr Phe 
    210                 215                 220                 


Gln Asp Pro Ala Leu Leu Leu Met His Glu Leu Ile His Val Leu His 
225                 230                 235                 240 


Gly Leu Tyr Gly Met Gln Val Ser Ser His Glu Ile Ile Pro Ser Lys 
                245                 250                 255     


Gln Glu Ile Tyr Met Gln His Thr Tyr Pro Ile Ser Ala Glu Glu Leu 
            260                 265                 270         


Phe Thr Phe Gly Gly Gln Asp Ala Asn Leu Ile Ser Ile Asp Ile Lys 
        275                 280                 285             


Asn Asp Leu Tyr Glu Lys Thr Leu Asn Asp Tyr Lys Ala Ile Ala Asn 
    290                 295                 300                 


Lys Leu Ser Gln Val Thr Ser Cys Asn Asp Pro Asn Ile Asp Ile Asp 
305                 310                 315                 320 


Ser Tyr Lys Gln Ile Tyr Gln Gln Lys Tyr Gln Phe Asp Lys Asp Ser 
                325                 330                 335     


Asn Gly Gln Tyr Ile Val Asn Glu Asp Lys Phe Gln Ile Leu Tyr Asn 
            340                 345                 350         


Ser Ile Met Tyr Gly Phe Thr Glu Ile Glu Leu Gly Lys Lys Phe Asn 
        355                 360                 365             


Ile Lys Thr Arg Leu Ser Tyr Phe Ser Met Asn His Asp Pro Val Lys 
    370                 375                 380                 


Ile Pro Asn Leu Leu Asp Asp Thr Ile Tyr Asn Asp Thr Glu Gly Phe 
385                 390                 395                 400 


Asn Ile Glu Ser Lys Asp Leu Lys Ser Glu Tyr Lys Gly Gln Asn Met 
                405                 410                 415     


Arg Val Asn Thr Asn Ala Phe Arg Asn Val Asp Gly Ser Gly Leu Val 
            420                 425                 430         


Ser Lys Leu Ile Gly Leu Cys Lys Lys Ile Ile Pro Pro Thr Asn Ile 
        435                 440                 445             


Arg Glu Asn Leu Tyr Asn Arg Thr Ala Ser Leu Thr Asp Leu Gly Gly 
    450                 455                 460                 


Glu Leu Cys Ile Lys Ile Lys Asn Glu Asp Leu Thr Phe Ile Ala Glu 
465                 470                 475                 480 


Lys Asn Ser Phe Ser Glu Glu Pro Phe Gln Asp Glu Ile Val Ser Tyr 
                485                 490                 495     


Asn Thr Lys Asn Lys Pro Leu Asn Phe Asn Tyr Ser Leu Asp Lys Ile 
            500                 505                 510         


Ile Val Asp Tyr Asn Leu Gln Ser Lys Ile Thr Leu Pro Asn Asp Arg 
        515                 520                 525             


Thr Thr Pro Val Thr Lys Gly Ile Pro Tyr Ala Pro Glu Tyr Lys Ser 
    530                 535                 540                 


Asn Ala Ala Ser Thr Ile Glu Ile His Asn Ile Asp Asp Asn Thr Ile 
545                 550                 555                 560 


Tyr Gln Tyr Leu Tyr Ala Gln Lys Ser Pro Thr Thr Leu Gln Arg Ile 
                565                 570                 575     


Thr Met Thr Asn Ser Val Asp Asp Ala Leu Ile Asn Ser Thr Lys Ile 
            580                 585                 590         


Tyr Ser Tyr Phe Pro Ser Val Ile Ser Lys Val Asn Gln Gly Ala Gln 
        595                 600                 605             


Gly Ile Leu Phe Leu Gln Trp Val Arg Asp Ile Ile Asp Asp Phe Thr 
    610                 615                 620                 


Asn Glu Ser Ser Gln Lys Thr Thr Ile Asp Lys Ile Ser Asp Val Ser 
625                 630                 635                 640 


Thr Ile Val Pro Tyr Ile Gly Pro Ala Leu Asn Ile Val Lys Gln Gly 
                645                 650                 655     


Tyr Glu Gly Asn Phe Ile Gly Ala Leu Glu Thr Thr Gly Val Val Leu 
            660                 665                 670         


Leu Leu Glu Tyr Ile Pro Glu Ile Thr Leu Pro Val Ile Ala Ala Leu 
        675                 680                 685             


Ser Ile Ala Glu Ser Ser Thr Gln Lys Glu Lys Ile Ile Lys Thr Ile 
    690                 695                 700                 


Asp Asn Phe Leu Glu Lys Arg Tyr Glu Lys Trp Ile Glu Val Tyr Lys 
705                 710                 715                 720 


Leu Val Lys Ala Lys Trp Leu Gly Thr Val Asn Thr Gln Phe Gln Lys 
                725                 730                 735     


Arg Ser Tyr Gln Met Tyr Arg Ser Leu Glu Tyr Gln Val Asp Ala Ile 
            740                 745                 750         


Lys Lys Ile Ile Asp Tyr Glu Tyr Lys Ile Tyr Ser Gly Pro Asp Lys 
        755                 760                 765             


Glu Gln Ile Ala Asp Glu Ile Asn Asn Leu Lys Asn Lys Leu Glu Glu 
    770                 775                 780                 


Lys Ala Asn Lys Ala Met Ile Asn Ile Asn Ile Phe Met Arg Glu Ser 
785                 790                 795                 800 


Ser Arg Ser Phe Leu Val Asn Gln Met Ile Asn Glu Ala Lys Lys Gln 
                805                 810                 815     


Leu Leu Glu Phe Asp Thr Gln Ser Lys Asn Ile Leu Met Gln Tyr Ile 
            820                 825                 830         


Lys Ala Asn Ser Lys Phe Ile Gly Ile Thr Glu Leu Lys Lys Leu Glu 
        835                 840                 845             


Ser Lys Ile Asn Lys Val Phe Ser Thr Pro Ile Pro Phe Ser Tyr Ser 
    850                 855                 860                 


Lys Asn Leu Asp Cys Trp Val Asp Asn Glu Glu Asp Ile Asp Val Ile 
865                 870                 875                 880 


Leu Lys Lys Ser Thr Ile Leu Asn Leu Asp Ile Asn Asn Asp Ile Ile 
                885                 890                 895     


Ser Asp Ile Ser Gly Phe Asn Ser Ser Val Ile Thr Tyr Pro Asp Ala 
            900                 905                 910         


Gln Leu Val Pro Gly Ile Asn Gly Lys Ala Ile His Leu Val Asn Asn 
        915                 920                 925             


Glu Ser Ser Glu Val Ile Val His Lys Ala Met Asp Ile Glu Tyr Asn 
    930                 935                 940                 


Asp Met Phe Asn Asn Phe Thr Val Ser Phe Trp Leu Arg Val Pro Lys 
945                 950                 955                 960 


Val Ser Ala Ser His Leu Glu Gln Tyr Gly Thr Asn Glu Tyr Ser Ile 
                965                 970                 975     


Ile Ser Ser Met Lys Lys His Ser Leu Ser Ile Gly Ser Gly Trp Ser 
            980                 985                 990         


Val Ser Leu Lys Gly Asn Asn Leu  Ile Trp Thr Leu Lys  Asp Ser Ala 
        995                 1000                 1005             


Gly Glu  Val Arg Gln Ile Thr  Phe Arg Asp Leu Pro  Asp Lys Phe 
    1010                 1015                 1020             


Asn Ala  Tyr Leu Ala Asn Lys  Trp Val Phe Ile Thr  Ile Thr Asn 
    1025                 1030                 1035             


Asp Arg  Leu Ser Ser Ala Asn  Leu Tyr Ile Asn Gly  Val Leu Met 
    1040                 1045                 1050             


Gly Ser  Ala Glu Ile Thr Gly  Leu Gly Ala Ile Arg  Glu Asp Asn 
    1055                 1060                 1065             


Asn Ile  Thr Leu Lys Leu Asp  Arg Cys Asn Asn Asn  Asn Gln Tyr 
    1070                 1075                 1080             


Val Ser  Ile Asp Lys Phe Arg  Ile Phe Cys Lys Ala  Leu Asn Pro 
    1085                 1090                 1095             


Lys Glu  Ile Glu Lys Leu Tyr  Thr Ser Tyr Leu Ser  Ile Thr Phe 
    1100                 1105                 1110             


Leu Arg  Asp Phe Trp Gly Asn  Pro Leu Arg Tyr Asp  Thr Glu Tyr 
    1115                 1120                 1125             


Tyr Leu  Ile Pro Val Ala Ser  Ser Ser Lys Asp Val  Gln Leu Lys 
    1130                 1135                 1140             


Asn Ile  Thr Asp Tyr Met Tyr  Leu Thr Asn Ala Pro  Ser Tyr Thr 
    1145                 1150                 1155             


Asn Gly  Lys Leu Asn Ile Tyr  Tyr Arg Arg Leu Tyr  Asn Gly Leu 
    1160                 1165                 1170             


Lys Phe  Ile Ile Lys Arg Tyr  Thr Pro Asn Asn Glu  Ile Asp Ser 
    1175                 1180                 1185             


Phe Val  Lys Ser Gly Asp Phe  Ile Lys Leu Tyr Val  Ser Tyr Asn 
    1190                 1195                 1200             


Asn Asn  Glu His Ile Val Gly  Tyr Pro Lys Asp Gly  Asn Ala Phe 
    1205                 1210                 1215             


Asn Asn  Leu Asp Arg Ile Leu  Arg Val Gly Tyr Asn  Ala Pro Gly 
    1220                 1225                 1230             


Ile Pro  Leu Tyr Lys Lys Met  Glu Ala Val Lys Leu  Arg Asp Leu 
    1235                 1240                 1245             


Lys Thr  Tyr Ser Val Gln Leu  Lys Leu Tyr Asp Asp  Lys Asn Ala 
    1250                 1255                 1260             


Ser Leu  Gly Leu Val Gly Thr  His Asn Gly Gln Ile  Gly Asn Asp 
    1265                 1270                 1275             


Pro Asn  Arg Asp Ile Leu Ile  Ala Ser Asn Trp Tyr  Phe Asn His 
    1280                 1285                 1290             


Leu Lys  Asp Lys Ile Leu Gly  Cys Asp Trp Tyr Phe  Val Pro Thr 
    1295                 1300                 1305             


Asp Glu  Gly Trp Thr Asn Asp  
    1310                 1315 


<210>  59
<211>  1306
<212>  PRT
<213>  Clostridium botulinum

<400>  59

Met Lys Leu Glu Ile Asn Lys Phe Asn Tyr Asn Asp Pro Ile Asp Gly 
1               5                   10                  15      


Ile Asn Val Ile Thr Met Arg Pro Pro Arg His Ser Asp Lys Ile Asn 
            20                  25                  30          


Lys Gly Lys Gly Pro Phe Lys Ala Phe Gln Val Ile Lys Asn Ile Trp 
        35                  40                  45              


Ile Val Pro Glu Arg Tyr Asn Phe Thr Asn Asn Thr Asn Asp Leu Asn 
    50                  55                  60                  


Ile Pro Ser Glu Pro Ile Met Glu Ala Asp Ala Ile Tyr Asn Pro Asn 
65                  70                  75                  80  


Tyr Leu Asn Thr Pro Ser Glu Lys Asp Glu Phe Leu Gln Gly Val Ile 
                85                  90                  95      


Lys Val Leu Glu Arg Ile Lys Ser Lys Pro Glu Gly Glu Lys Leu Leu 
            100                 105                 110         


Glu Leu Ile Ser Ser Ser Ile Pro Leu Pro Leu Val Ser Asn Gly Ala 
        115                 120                 125             


Leu Thr Leu Ser Asp Asn Glu Thr Ile Ala Tyr Gln Glu Asn Asn Asn 
    130                 135                 140                 


Ile Val Ser Asn Leu Gln Ala Asn Leu Val Ile Tyr Gly Pro Gly Pro 
145                 150                 155                 160 


Asp Ile Ala Asn Asn Ala Thr Tyr Gly Leu Tyr Ser Thr Pro Ile Ser 
                165                 170                 175     


Asn Gly Glu Gly Thr Leu Ser Glu Val Ser Phe Ser Pro Phe Tyr Leu 
            180                 185                 190         


Lys Pro Phe Asp Glu Ser Tyr Gly Asn Tyr Arg Ser Leu Val Asn Ile 
        195                 200                 205             


Val Asn Lys Phe Val Lys Arg Glu Phe Ala Pro Asp Pro Ala Ser Thr 
    210                 215                 220                 


Leu Met His Glu Leu Val His Val Thr His Asn Leu Tyr Gly Ile Ser 
225                 230                 235                 240 


Asn Arg Asn Phe Tyr Tyr Asn Phe Asp Thr Gly Lys Ile Glu Thr Ser 
                245                 250                 255     


Arg Gln Gln Asn Ser Leu Ile Phe Glu Glu Leu Leu Thr Phe Gly Gly 
            260                 265                 270         


Ile Asp Ser Lys Ala Ile Ser Ser Leu Ile Ile Lys Lys Ile Ile Glu 
        275                 280                 285             


Thr Ala Lys Asn Asn Tyr Thr Thr Leu Ile Ser Glu Arg Leu Asn Thr 
    290                 295                 300                 


Val Thr Val Glu Asn Asp Leu Leu Lys Tyr Ile Lys Asn Lys Ile Pro 
305                 310                 315                 320 


Val Gln Gly Arg Leu Gly Asn Phe Lys Leu Asp Thr Ala Glu Phe Glu 
                325                 330                 335     


Lys Lys Leu Asn Thr Ile Leu Phe Val Leu Asn Glu Ser Asn Leu Ala 
            340                 345                 350         


Gln Arg Phe Ser Ile Leu Val Arg Lys His Tyr Leu Lys Glu Arg Pro 
        355                 360                 365             


Ile Asp Pro Ile Tyr Val Asn Ile Leu Asp Asp Asn Ser Tyr Ser Thr 
    370                 375                 380                 


Leu Glu Gly Phe Asn Ile Ser Ser Gln Gly Ser Asn Asp Phe Gln Gly 
385                 390                 395                 400 


Gln Leu Leu Glu Ser Ser Tyr Phe Glu Lys Ile Glu Ser Asn Ala Leu 
                405                 410                 415     


Arg Ala Phe Ile Lys Ile Cys Pro Arg Asn Gly Leu Leu Tyr Asn Ala 
            420                 425                 430         


Ile Tyr Arg Asn Ser Lys Asn Tyr Leu Asn Asn Ile Asp Leu Glu Asp 
        435                 440                 445             


Lys Lys Thr Thr Ser Lys Thr Asn Val Ser Tyr Pro Cys Ser Leu Leu 
    450                 455                 460                 


Asn Gly Cys Ile Glu Val Glu Asn Lys Asp Leu Phe Leu Ile Ser Asn 
465                 470                 475                 480 


Lys Asp Ser Leu Asn Asp Ile Asn Leu Ser Glu Glu Lys Ile Lys Pro 
                485                 490                 495     


Glu Thr Thr Val Phe Phe Lys Asp Lys Leu Pro Pro Gln Asp Ile Thr 
            500                 505                 510         


Leu Ser Asn Tyr Asp Phe Thr Glu Ala Asn Ser Ile Pro Ser Ile Ser 
        515                 520                 525             


Gln Gln Asn Ile Leu Glu Arg Asn Glu Glu Leu Tyr Glu Pro Ile Arg 
    530                 535                 540                 


Asn Ser Leu Phe Glu Ile Lys Thr Ile Tyr Val Asp Lys Leu Thr Thr 
545                 550                 555                 560 


Phe His Phe Leu Glu Ala Gln Asn Ile Asp Glu Ser Ile Asp Ser Ser 
                565                 570                 575     


Lys Ile Arg Val Glu Leu Thr Asp Ser Val Asp Glu Ala Leu Ser Asn 
            580                 585                 590         


Pro Asn Lys Val Tyr Ser Pro Phe Lys Asn Met Ser Asn Thr Ile Asn 
        595                 600                 605             


Ser Ile Glu Thr Gly Ile Thr Ser Thr Tyr Ile Phe Tyr Gln Trp Leu 
    610                 615                 620                 


Arg Ser Ile Val Lys Asp Phe Ser Asp Glu Thr Gly Lys Ile Asp Val 
625                 630                 635                 640 


Ile Asp Lys Ser Ser Asp Thr Leu Ala Ile Val Pro Tyr Ile Gly Pro 
                645                 650                 655     


Leu Leu Asn Ile Gly Asn Asp Ile Arg His Gly Asp Phe Val Gly Ala 
            660                 665                 670         


Ile Glu Leu Ala Gly Ile Thr Ala Leu Leu Glu Tyr Val Pro Glu Phe 
        675                 680                 685             


Thr Ile Pro Ile Leu Val Gly Leu Glu Val Ile Gly Gly Glu Leu Ala 
    690                 695                 700                 


Arg Glu Gln Val Glu Ala Ile Val Asn Asn Ala Leu Asp Lys Arg Asp 
705                 710                 715                 720 


Gln Lys Trp Ala Glu Val Tyr Asn Ile Thr Lys Ala Gln Trp Trp Gly 
                725                 730                 735     


Thr Ile His Leu Gln Ile Asn Thr Arg Leu Ala His Thr Tyr Lys Ala 
            740                 745                 750         


Leu Ser Arg Gln Ala Asn Ala Ile Lys Met Asn Met Glu Phe Gln Leu 
        755                 760                 765             


Ala Asn Tyr Lys Gly Asn Ile Asp Asp Lys Ala Lys Ile Lys Asn Ala 
    770                 775                 780                 


Ile Ser Glu Thr Glu Ile Leu Leu Asn Lys Ser Val Glu Gln Ala Met 
785                 790                 795                 800 


Lys Asn Thr Glu Lys Phe Met Ile Lys Leu Ser Asn Ser Tyr Leu Thr 
                805                 810                 815     


Lys Glu Met Ile Pro Lys Val Gln Asp Asn Leu Lys Asn Phe Asp Leu 
            820                 825                 830         


Glu Thr Lys Lys Thr Leu Asp Lys Phe Ile Lys Glu Lys Glu Asp Ile 
        835                 840                 845             


Leu Gly Thr Asn Leu Ser Ser Ser Leu Arg Arg Lys Val Ser Ile Arg 
    850                 855                 860                 


Leu Asn Lys Asn Ile Ala Phe Asp Ile Asn Asp Ile Pro Phe Ser Glu 
865                 870                 875                 880 


Phe Asp Asp Leu Ile Asn Gln Tyr Lys Asn Glu Ile Glu Asp Tyr Glu 
                885                 890                 895     


Val Leu Asn Leu Gly Ala Glu Asp Gly Lys Ile Lys Asp Leu Ser Gly 
            900                 905                 910         


Thr Thr Ser Asp Ile Asn Ile Gly Ser Asp Ile Glu Leu Ala Asp Gly 
        915                 920                 925             


Arg Glu Asn Lys Ala Ile Lys Ile Lys Gly Ser Glu Asn Ser Thr Ile 
    930                 935                 940                 


Lys Ile Ala Met Asn Lys Tyr Leu Arg Phe Ser Ala Thr Asp Asn Phe 
945                 950                 955                 960 


Ser Ile Ser Phe Trp Ile Lys His Pro Lys Pro Thr Asn Leu Leu Asn 
                965                 970                 975     


Asn Gly Ile Glu Tyr Thr Leu Val Glu Asn Phe Asn Gln Arg Gly Trp 
            980                 985                 990         


Lys Ile Ser Ile Gln Asp Ser Lys  Leu Ile Trp Tyr Leu  Arg Asp His 
        995                 1000                 1005             


Asn Asn  Ser Ile Lys Ile Val  Thr Pro Asp Tyr Ile  Ala Phe Asn 
    1010                 1015                 1020             


Gly Trp  Asn Leu Ile Thr Ile  Thr Asn Asn Arg Ser  Lys Gly Ser 
    1025                 1030                 1035             


Ile Val  Tyr Val Asn Gly Ser  Lys Ile Glu Glu Lys  Asp Ile Ser 
    1040                 1045                 1050             


Ser Ile  Trp Asn Thr Glu Val  Asp Asp Pro Ile Ile  Phe Arg Leu 
    1055                 1060                 1065             


Lys Asn  Asn Arg Asp Thr Gln  Ala Phe Thr Leu Leu  Asp Gln Phe 
    1070                 1075                 1080             


Ser Ile  Tyr Arg Lys Glu Leu  Asn Gln Asn Glu Val  Val Lys Leu 
    1085                 1090                 1095             


Tyr Asn  Tyr Tyr Phe Asn Ser  Asn Tyr Ile Arg Asp  Ile Trp Gly 
    1100                 1105                 1110             


Asn Pro  Leu Gln Tyr Asn Lys  Lys Tyr Tyr Leu Gln  Thr Gln Asp 
    1115                 1120                 1125             


Lys Pro  Gly Lys Gly Leu Ile  Arg Glu Tyr Trp Ser  Ser Phe Gly 
    1130                 1135                 1140             


Tyr Asp  Tyr Val Ile Leu Ser  Asp Ser Lys Thr Ile  Thr Phe Pro 
    1145                 1150                 1155             


Asn Asn  Ile Arg Tyr Gly Ala  Leu Tyr Asn Gly Ser  Lys Val Leu 
    1160                 1165                 1170             


Ile Lys  Asn Ser Lys Lys Leu  Asp Gly Leu Val Arg  Asn Lys Asp 
    1175                 1180                 1185             


Phe Ile  Gln Leu Glu Ile Asp  Gly Tyr Asn Met Gly  Ile Ser Ala 
    1190                 1195                 1200             


Asp Arg  Phe Asn Glu Asp Thr  Asn Tyr Ile Gly Thr  Thr Tyr Gly 
    1205                 1210                 1215             


Thr Thr  His Asp Leu Thr Thr  Asp Phe Glu Ile Ile  Gln Arg Gln 
    1220                 1225                 1230             


Glu Lys  Tyr Arg Asn Tyr Cys  Gln Leu Lys Thr Pro  Tyr Asn Ile 
    1235                 1240                 1245             


Phe His  Lys Ser Gly Leu Met  Ser Thr Glu Thr Ser  Lys Pro Thr 
    1250                 1255                 1260             


Phe His  Asp Tyr Arg Asp Trp  Val Tyr Ser Ser Ala  Trp Tyr Phe 
    1265                 1270                 1275             


Gln Asn  Tyr Glu Asn Leu Asn  Leu Arg Lys His Thr  Lys Thr Asn 
    1280                 1285                 1290             


Trp Tyr  Phe Ile Pro Lys Asp  Glu Gly Trp Asp Glu  Asp 
    1295                 1300                 1305     


<210>  60
<211>  3888
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide Sequence of mrBoNT/A

<400>  60
atgccattcg tcaacaagca attcaactac aaagacccag tcaacggcgt cgacatcgca       60

tacatcaaga ttccgaacgc cggtcaaatg cagccggtta aggcttttaa gatccacaac      120

aagatttggg ttatcccgga gcgtgacacc ttcacgaacc cggaagaagg cgatctgaac      180

ccgccaccgg aagcgaagca agtccctgtc agctactacg attcgacgta cctgagcacg      240

gataacgaaa aagataacta cctgaaaggt gtgaccaagc tgttcgaacg tatctacagc      300

acggatctgg gtcgcatgct gctgactagc attgttcgcg gtatcccgtt ctggggtggt      360

agcacgattg acaccgaact gaaggttatc gacactaact gcattaacgt tattcaaccg      420

gatggtagct atcgtagcga agagctgaat ctggtcatca ttggcccgag cgcagacatt      480

atccaattcg agtgcaagag ctttggtcac gaggttctga atctgacccg caatggctat      540

ggtagcaccc agtacattcg tttttcgccg gattttacct tcggctttga agagagcctg      600

gaggttgata ccaatccgtt gctgggtgcg ggcaaattcg ctaccgatcc ggctgtcacg      660

ctggcccatg aactgatcca cgcaggccac cgcctgtacg gcattgccat caacccaaac      720

cgtgtgttca aggttaatac gaatgcatac tacgagatga gcggcctgga agtcagcttc      780

gaagaactgc gcaccttcgg tggccatgac gctaaattca ttgacagctt gcaagagaat      840

gagttccgtc tgtactacta taacaaattc aaagacattg caagcacgtt gaacaaggcc      900

aaaagcatcg ttggtactac cgcgtcgttg cagtatatga agaatgtgtt taaagagaag      960

tacctgctgt ccgaggatac ctccggcaag tttagcgttg ataagctgaa gtttgacaaa     1020

ctgtacaaga tgctgaccga gatttacacc gaggacaact ttgtgaaatt cttcaaagtg     1080

ttgaatcgta aaacctatct gaattttgac aaagcggttt tcaagattaa catcgtgccg     1140

aaggtgaact acaccatcta tgacggtttt aacctgcgta acaccaacct ggcggcgaac     1200

tttaacggtc agaatacgga aatcaacaac atgaatttca cgaagttgaa gaacttcacg     1260

ggtctgttcg agttctataa gctgctgtgc gtgcgcggta tcatcaccag caaaaccaaa     1320

agcctggaca aaggctacaa caaggcgctg aatgacctgt gcattaaggt aaacaattgg     1380

gatctgttct tttcgccatc cgaagataat tttaccaacg acctgaacaa gggtgaagaa     1440

atcaccagcg atacgaatat tgaagcagcg gaagagaata tcagcctgga tctgatccag     1500

cagtactatc tgacctttaa cttcgacaat gaaccggaga acattagcat tgagaatctg     1560

agcagcgaca ttatcggtca gctggaactg atgccgaata tcgaacgttt cccgaacggc     1620

aaaaagtacg agctggacaa gtacactatg ttccattacc tgcgtgcaca ggagtttgaa     1680

cacggtaaaa gccgtatcgc gctgaccaac agcgttaacg aggccctgct gaacccgagc     1740

cgtgtctata ccttcttcag cagcgactat gttaagaaag tgaacaaagc cactgaggcc     1800

gcgatgttcc tgggctgggt ggaacagctg gtatatgact tcacggacga gacgagcgaa     1860

gtgagcacta ccgacaaaat tgctgatatt accatcatta tcccgtatat tggtccggca     1920

ctgaacattg gcaacatgct gtacaaagac gattttgtgg gtgccctgat cttctccggt     1980

gccgtgattc tgctggagtt cattccggag attgcgatcc cggtgttggg taccttcgcg     2040

ctggtgtcct acatcgcgaa taaggttctg acggttcaga ccatcgataa cgcgctgtcg     2100

aaacgtaatg aaaaatggga cgaggtttac aaatacattg ttacgaattg gctggcgaaa     2160

gtcaataccc agatcgacct gatccgtaag aaaatgaaag aggcgctgga gaatcaggcg     2220

gaggccacca aagcaattat caactaccaa tacaaccagt acacggaaga agagaagaat     2280

aacattaact tcaatatcga tgatttgagc agcaagctga atgaatctat caacaaagcg     2340

atgatcaata tcaacaagtt tttgaatcag tgtagcgttt cgtacctgat gaatagcatg     2400

attccgtatg gcgtcaaacg tctggaggac ttcgacgcca gcctgaaaga tgcgttgctg     2460

aaatacattt acgacaatcg tggtacgctg attggccaag ttgaccgctt gaaagacaaa     2520

gttaacaata ccctgagcac cgacatccca tttcaactga gcaagtatgt tgataatcaa     2580

cgtctgttga gcactttcac cgagtatatc aaaaacatca tcaatactag cattctgaac     2640

ctgcgttacg agagcaagca tctgattgat ctgagccgtt atgctagcaa gatcaacatc     2700

ggtagcaagg tcaattttga cccgatcgat aagaaccaga tccagctgtt taatctggaa     2760

tcgagcaaaa ttgaggttat cctgaaaaag gccattgtct acaactccat gtacgagaat     2820

ttctccacca gcttctggat tcgcatcccg aaatacttca acaagattag cctgaacaac     2880

gagtatacta tcatcaactg tatggagaac aacagcggtt ggaaggtgtc tctgaactat     2940

ggtgagatca tttggacctt gcaggacacc aaagagatca agcagcgcgt cgtgttcaag     3000

tactctcaaa tgatcaacat ttccgattac attaatcgtt ggatcttcgt gaccattacg     3060

aataaccgtc tgaataagag caagatttac atcaatggtc gcttgatcga tcagaaaccg     3120

attagcaacc tgggtaatat ccacgcaagc aacaagatta tgttcaaatt ggacggttgc     3180

cgcgataccc atcgttatat ctggatcaag tatttcaacc tgtttgataa agaactgaat     3240

gagaaggaga tcaaagattt gtatgacaac caatctaaca gcggcatttt gaaggacttc     3300

tggggcgatt atctgcaata cgataagccg tactatatgc tgaacctgta tgatccgaac     3360

aaatatgtgg atgtcaataa tgtgggtatt cgtggttaca tgtatttgaa gggtccgcgt     3420

ggcagcgtta tgacgaccaa catttacctg aactctagcc tgtaccgtgg tacgaaattc     3480

atcattaaga aatatgccag cggcaacaaa gataacattg tgcgtaataa cgatcgtgtc     3540

tacatcaacg tggtcgtgaa gaataaagag taccgtctgg cgaccaacgc ttcgcaggcg     3600

ggtgttgaga aaattctgag cgcgttggag atccctgatg tcggtaatct gagccaagtc     3660

gtggttatga agagcaagaa cgacaagggt atcactaaca agtgcaagat gaacctgcaa     3720

gacaacaatg gtaacgacat cggctttatt ggtttccacc agttcaacaa tattgctaaa     3780

ctggtagcga gcaattggta caatcgtcag attgagcgca gcagccgtac tttgggctgt     3840

agctgggagt ttatcccggt cgatgatggt tggggcgaac gtccgctg                  3888


<210>  61
<211>  1296
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide Sequence of mrBoNT/A

<400>  61

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly Tyr Asn Lys 
        435                 440                 445             


Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe 
    450                 455                 460                 


Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu 
465                 470                 475                 480 


Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu 
                485                 490                 495     


Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro 
            500                 505                 510         


Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu 
        515                 520                 525             


Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu 
    530                 535                 540                 


Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu 
545                 550                 555                 560 


His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu 
                565                 570                 575     


Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys 
            580                 585                 590         


Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu 
        595                 600                 605             


Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr 
    610                 615                 620                 


Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala 
625                 630                 635                 640 


Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu 
                645                 650                 655     


Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala 
            660                 665                 670         


Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys 
        675                 680                 685             


Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu 
    690                 695                 700                 


Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys 
705                 710                 715                 720 


Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu 
                725                 730                 735     


Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn 
            740                 745                 750         


Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp 
        755                 760                 765             


Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile 
    770                 775                 780                 


Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met 
785                 790                 795                 800 


Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys 
                805                 810                 815     


Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly 
            820                 825                 830         


Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp 
        835                 840                 845             


Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser 
    850                 855                 860                 


Thr Phe Thr Glu Tyr Ile Lys Asn Ile Ile Asn Thr Ser Ile Leu Asn 
865                 870                 875                 880 


Leu Arg Tyr Glu Ser Lys His Leu Ile Asp Leu Ser Arg Tyr Ala Ser 
                885                 890                 895     


Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp Pro Ile Asp Lys Asn 
            900                 905                 910         


Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys Ile Glu Val Ile Leu 
        915                 920                 925             


Lys Lys Ala Ile Val Tyr Asn Ser Met Tyr Glu Asn Phe Ser Thr Ser 
    930                 935                 940                 


Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn Lys Ile Ser Leu Asn Asn 
945                 950                 955                 960 


Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn Ser Gly Trp Lys Val 
                965                 970                 975     


Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr Leu Gln Asp Thr Lys Glu 
            980                 985                 990         


Ile Lys Gln Arg Val Val Phe Lys  Tyr Ser Gln Met Ile  Asn Ile Ser 
        995                 1000                 1005             


Asp Tyr  Ile Asn Arg Trp Ile  Phe Val Thr Ile Thr  Asn Asn Arg 
    1010                 1015                 1020             


Leu Asn  Lys Ser Lys Ile Tyr  Ile Asn Gly Arg Leu  Ile Asp Gln 
    1025                 1030                 1035             


Lys Pro  Ile Ser Asn Leu Gly  Asn Ile His Ala Ser  Asn Lys Ile 
    1040                 1045                 1050             


Met Phe  Lys Leu Asp Gly Cys  Arg Asp Thr His Arg  Tyr Ile Trp 
    1055                 1060                 1065             


Ile Lys  Tyr Phe Asn Leu Phe  Asp Lys Glu Leu Asn  Glu Lys Glu 
    1070                 1075                 1080             


Ile Lys  Asp Leu Tyr Asp Asn  Gln Ser Asn Ser Gly  Ile Leu Lys 
    1085                 1090                 1095             


Asp Phe  Trp Gly Asp Tyr Leu  Gln Tyr Asp Lys Pro  Tyr Tyr Met 
    1100                 1105                 1110             


Leu Asn  Leu Tyr Asp Pro Asn  Lys Tyr Val Asp Val  Asn Asn Val 
    1115                 1120                 1125             


Gly Ile  Arg Gly Tyr Met Tyr  Leu Lys Gly Pro Arg  Gly Ser Val 
    1130                 1135                 1140             


Met Thr  Thr Asn Ile Tyr Leu  Asn Ser Ser Leu Tyr  Arg Gly Thr 
    1145                 1150                 1155             


Lys Phe  Ile Ile Lys Lys Tyr  Ala Ser Gly Asn Lys  Asp Asn Ile 
    1160                 1165                 1170             


Val Arg  Asn Asn Asp Arg Val  Tyr Ile Asn Val Val  Val Lys Asn 
    1175                 1180                 1185             


Lys Glu  Tyr Arg Leu Ala Thr  Asn Ala Ser Gln Ala  Gly Val Glu 
    1190                 1195                 1200             


Lys Ile  Leu Ser Ala Leu Glu  Ile Pro Asp Val Gly  Asn Leu Ser 
    1205                 1210                 1215             


Gln Val  Val Val Met Lys Ser  Lys Asn Asp Lys Gly  Ile Thr Asn 
    1220                 1225                 1230             


Lys Cys  Lys Met Asn Leu Gln  Asp Asn Asn Gly Asn  Asp Ile Gly 
    1235                 1240                 1245             


Phe Ile  Gly Phe His Gln Phe  Asn Asn Ile Ala Lys  Leu Val Ala 
    1250                 1255                 1260             


Ser Asn  Trp Tyr Asn Arg Gln  Ile Glu Arg Ser Ser  Arg Thr Leu 
    1265                 1270                 1275             


Gly Cys  Ser Trp Glu Phe Ile  Pro Val Asp Asp Gly  Trp Gly Glu 
    1280                 1285                 1290             


Arg Pro  Leu 
    1295     


<210>  62
<211>  1296
<212>  PRT
<213>  Clostridium botulinum

<400>  62

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly Tyr Asn Lys 
        435                 440                 445             


Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe 
    450                 455                 460                 


Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu 
465                 470                 475                 480 


Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu 
                485                 490                 495     


Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro 
            500                 505                 510         


Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu 
        515                 520                 525             


Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu 
    530                 535                 540                 


Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu 
545                 550                 555                 560 


His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu 
                565                 570                 575     


Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys 
            580                 585                 590         


Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu 
        595                 600                 605             


Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr 
    610                 615                 620                 


Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala 
625                 630                 635                 640 


Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu 
                645                 650                 655     


Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala 
            660                 665                 670         


Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys 
        675                 680                 685             


Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu 
    690                 695                 700                 


Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys 
705                 710                 715                 720 


Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu 
                725                 730                 735     


Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn 
            740                 745                 750         


Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp 
        755                 760                 765             


Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile 
    770                 775                 780                 


Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met 
785                 790                 795                 800 


Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys 
                805                 810                 815     


Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly 
            820                 825                 830         


Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp 
        835                 840                 845             


Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser 
    850                 855                 860                 


Thr Phe Thr Glu Tyr Ile Lys Asn Ile Ile Asn Thr Ser Ile Leu Asn 
865                 870                 875                 880 


Leu Arg Tyr Glu Ser Asn His Leu Ile Asp Leu Ser Arg Tyr Ala Ser 
                885                 890                 895     


Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp Pro Ile Asp Lys Asn 
            900                 905                 910         


Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys Ile Glu Val Ile Leu 
        915                 920                 925             


Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr Glu Asn Phe Ser Thr Ser 
    930                 935                 940                 


Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn Ser Ile Ser Leu Asn Asn 
945                 950                 955                 960 


Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn Ser Gly Trp Lys Val 
                965                 970                 975     


Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr Leu Gln Asp Thr Gln Glu 
            980                 985                 990         


Ile Lys Gln Arg Val Val Phe Lys  Tyr Ser Gln Met Ile  Asn Ile Ser 
        995                 1000                 1005             


Asp Tyr  Ile Asn Arg Trp Ile  Phe Val Thr Ile Thr  Asn Asn Arg 
    1010                 1015                 1020             


Leu Asn  Asn Ser Lys Ile Tyr  Ile Asn Gly Arg Leu  Ile Asp Gln 
    1025                 1030                 1035             


Lys Pro  Ile Ser Asn Leu Gly  Asn Ile His Ala Ser  Asn Asn Ile 
    1040                 1045                 1050             


Met Phe  Lys Leu Asp Gly Cys  Arg Asp Thr His Arg  Tyr Ile Trp 
    1055                 1060                 1065             


Ile Lys  Tyr Phe Asn Leu Phe  Asp Lys Glu Leu Asn  Glu Lys Glu 
    1070                 1075                 1080             


Ile Lys  Asp Leu Tyr Asp Asn  Gln Ser Asn Ser Gly  Ile Leu Lys 
    1085                 1090                 1095             


Asp Phe  Trp Gly Asp Tyr Leu  Gln Tyr Asp Lys Pro  Tyr Tyr Met 
    1100                 1105                 1110             


Leu Asn  Leu Tyr Asp Pro Asn  Lys Tyr Val Asp Val  Asn Asn Val 
    1115                 1120                 1125             


Gly Ile  Arg Gly Tyr Met Tyr  Leu Lys Gly Pro Arg  Gly Ser Val 
    1130                 1135                 1140             


Met Thr  Thr Asn Ile Tyr Leu  Asn Ser Ser Leu Tyr  Arg Gly Thr 
    1145                 1150                 1155             


Lys Phe  Ile Ile Lys Lys Tyr  Ala Ser Gly Asn Lys  Asp Asn Ile 
    1160                 1165                 1170             


Val Arg  Asn Asn Asp Arg Val  Tyr Ile Asn Val Val  Val Lys Asn 
    1175                 1180                 1185             


Lys Glu  Tyr Arg Leu Ala Thr  Asn Ala Ser Gln Ala  Gly Val Glu 
    1190                 1195                 1200             


Lys Ile  Leu Ser Ala Leu Glu  Ile Pro Asp Val Gly  Asn Leu Ser 
    1205                 1210                 1215             


Gln Val  Val Val Met Lys Ser  Lys Asn Asp Gln Gly  Ile Thr Asn 
    1220                 1225                 1230             


Lys Cys  Lys Met Asn Leu Gln  Asp Asn Asn Gly Asn  Asp Ile Gly 
    1235                 1240                 1245             


Phe Ile  Gly Phe His Gln Phe  Asn Asn Ile Ala Lys  Leu Val Ala 
    1250                 1255                 1260             


Ser Asn  Trp Tyr Asn Arg Gln  Ile Glu Arg Ser Ser  Arg Thr Leu 
    1265                 1270                 1275             


Gly Cys  Ser Trp Glu Phe Ile  Pro Val Asp Asp Gly  Trp Gly Glu 
    1280                 1285                 1290             


Arg Pro  Leu 
    1295     


<210>  63
<211>  1304
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide Sequence of mrBoNT/AB

<400>  63

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly Tyr Asn Lys 
        435                 440                 445             


Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe 
    450                 455                 460                 


Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu 
465                 470                 475                 480 


Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu 
                485                 490                 495     


Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro 
            500                 505                 510         


Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu 
        515                 520                 525             


Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu 
    530                 535                 540                 


Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu 
545                 550                 555                 560 


His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu 
                565                 570                 575     


Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys 
            580                 585                 590         


Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu 
        595                 600                 605             


Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr 
    610                 615                 620                 


Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala 
625                 630                 635                 640 


Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu 
                645                 650                 655     


Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala 
            660                 665                 670         


Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys 
        675                 680                 685             


Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu 
    690                 695                 700                 


Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys 
705                 710                 715                 720 


Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu 
                725                 730                 735     


Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn 
            740                 745                 750         


Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp 
        755                 760                 765             


Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile 
    770                 775                 780                 


Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met 
785                 790                 795                 800 


Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys 
                805                 810                 815     


Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly 
            820                 825                 830         


Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp 
        835                 840                 845             


Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser 
    850                 855                 860                 


Thr Phe Thr Glu Tyr Ile Lys Asn Ile Leu Asn Asn Ile Ile Leu Asn 
865                 870                 875                 880 


Leu Arg Tyr Lys Asp Asn Asn Leu Ile Asp Leu Ser Gly Tyr Gly Ala 
                885                 890                 895     


Lys Val Glu Val Tyr Asp Gly Val Glu Leu Asn Asp Lys Asn Gln Phe 
            900                 905                 910         


Lys Leu Thr Ser Ser Ala Asn Ser Lys Ile Arg Val Thr Gln Asn Gln 
        915                 920                 925             


Asn Ile Ile Phe Asn Ser Val Phe Leu Asp Phe Ser Val Ser Phe Trp 
    930                 935                 940                 


Ile Arg Ile Pro Lys Tyr Lys Asn Asp Gly Ile Gln Asn Tyr Ile His 
945                 950                 955                 960 


Asn Glu Tyr Thr Ile Ile Asn Cys Met Lys Asn Asn Ser Gly Trp Lys 
                965                 970                 975     


Ile Ser Ile Arg Gly Asn Arg Ile Ile Trp Thr Leu Ile Asp Ile Asn 
            980                 985                 990         


Gly Lys Thr Lys Ser Val Phe Phe  Glu Tyr Asn Ile Arg  Glu Asp Ile 
        995                 1000                 1005             


Ser Glu  Tyr Ile Asn Arg Trp  Phe Phe Val Thr Ile  Thr Asn Asn 
    1010                 1015                 1020             


Leu Asn  Asn Ala Lys Ile Tyr  Ile Asn Gly Lys Leu  Glu Ser Asn 
    1025                 1030                 1035             


Thr Asp  Ile Lys Asp Ile Arg  Glu Val Ile Ala Asn  Gly Glu Ile 
    1040                 1045                 1050             


Ile Phe  Lys Leu Asp Gly Asp  Ile Asp Arg Thr Gln  Phe Ile Trp 
    1055                 1060                 1065             


Met Lys  Tyr Phe Ser Ile Phe  Asn Thr Glu Leu Ser  Gln Ser Asn 
    1070                 1075                 1080             


Ile Glu  Glu Arg Tyr Lys Ile  Gln Ser Tyr Ser Glu  Tyr Leu Lys 
    1085                 1090                 1095             


Asp Phe  Trp Gly Asn Pro Leu  Met Tyr Asn Lys Glu  Tyr Tyr Met 
    1100                 1105                 1110             


Phe Asn  Ala Gly Asn Lys Asn  Ser Tyr Ile Lys Leu  Lys Lys Asp 
    1115                 1120                 1125             


Ser Pro  Val Gly Glu Ile Leu  Thr Arg Ser Lys Tyr  Asn Gln Asn 
    1130                 1135                 1140             


Ser Lys  Tyr Ile Asn Tyr Arg  Asp Leu Tyr Ile Gly  Glu Lys Phe 
    1145                 1150                 1155             


Ile Ile  Arg Arg Lys Ser Asn  Ser Gln Ser Ile Asn  Asp Asp Ile 
    1160                 1165                 1170             


Val Arg  Lys Glu Asp Tyr Ile  Tyr Leu Asp Phe Phe  Asn Leu Asn 
    1175                 1180                 1185             


Gln Glu  Trp Arg Val Tyr Thr  Tyr Lys Tyr Phe Lys  Lys Glu Glu 
    1190                 1195                 1200             


Met Lys  Leu Phe Leu Ala Pro  Ile Tyr Asp Ser Asp  Glu Phe Tyr 
    1205                 1210                 1215             


Asn Thr  Ile Gln Ile Lys Glu  Tyr Asp Glu Gln Pro  Thr Tyr Ser 
    1220                 1225                 1230             


Cys Gln  Leu Leu Phe Lys Lys  Asp Glu Glu Ser Thr  Asp Glu Ile 
    1235                 1240                 1245             


Gly Leu  Ile Gly Ile His Arg  Phe Tyr Glu Ser Gly  Ile Val Phe 
    1250                 1255                 1260             


Glu Glu  Tyr Lys Asp Tyr Phe  Cys Ile Ser Lys Trp  Tyr Leu Lys 
    1265                 1270                 1275             


Glu Val  Lys Arg Lys Pro Tyr  Asn Leu Lys Leu Gly  Cys Asn Trp 
    1280                 1285                 1290             


Gln Phe  Ile Pro Lys Asp Glu  Gly Trp Thr Glu 
    1295                 1300                 


<210>  64
<211>  1304
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide Sequence of mrBoNT/AB(0)

<400>  64

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Gln 
    210                 215                 220                 


Leu Ile Tyr Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly Tyr Asn Lys 
        435                 440                 445             


Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe 
    450                 455                 460                 


Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu 
465                 470                 475                 480 


Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu 
                485                 490                 495     


Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro 
            500                 505                 510         


Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu 
        515                 520                 525             


Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu 
    530                 535                 540                 


Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu 
545                 550                 555                 560 


His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu 
                565                 570                 575     


Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys 
            580                 585                 590         


Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu 
        595                 600                 605             


Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr 
    610                 615                 620                 


Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala 
625                 630                 635                 640 


Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu 
                645                 650                 655     


Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala 
            660                 665                 670         


Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys 
        675                 680                 685             


Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu 
    690                 695                 700                 


Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys 
705                 710                 715                 720 


Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu 
                725                 730                 735     


Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn 
            740                 745                 750         


Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp 
        755                 760                 765             


Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile 
    770                 775                 780                 


Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met 
785                 790                 795                 800 


Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys 
                805                 810                 815     


Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly 
            820                 825                 830         


Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp 
        835                 840                 845             


Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser 
    850                 855                 860                 


Thr Phe Thr Glu Tyr Ile Lys Asn Ile Leu Asn Asn Ile Ile Leu Asn 
865                 870                 875                 880 


Leu Arg Tyr Lys Asp Asn Asn Leu Ile Asp Leu Ser Gly Tyr Gly Ala 
                885                 890                 895     


Lys Val Glu Val Tyr Asp Gly Val Glu Leu Asn Asp Lys Asn Gln Phe 
            900                 905                 910         


Lys Leu Thr Ser Ser Ala Asn Ser Lys Ile Arg Val Thr Gln Asn Gln 
        915                 920                 925             


Asn Ile Ile Phe Asn Ser Val Phe Leu Asp Phe Ser Val Ser Phe Trp 
    930                 935                 940                 


Ile Arg Ile Pro Lys Tyr Lys Asn Asp Gly Ile Gln Asn Tyr Ile His 
945                 950                 955                 960 


Asn Glu Tyr Thr Ile Ile Asn Cys Met Lys Asn Asn Ser Gly Trp Lys 
                965                 970                 975     


Ile Ser Ile Arg Gly Asn Arg Ile Ile Trp Thr Leu Ile Asp Ile Asn 
            980                 985                 990         


Gly Lys Thr Lys Ser Val Phe Phe  Glu Tyr Asn Ile Arg  Glu Asp Ile 
        995                 1000                 1005             


Ser Glu  Tyr Ile Asn Arg Trp  Phe Phe Val Thr Ile  Thr Asn Asn 
    1010                 1015                 1020             


Leu Asn  Asn Ala Lys Ile Tyr  Ile Asn Gly Lys Leu  Glu Ser Asn 
    1025                 1030                 1035             


Thr Asp  Ile Lys Asp Ile Arg  Glu Val Ile Ala Asn  Gly Glu Ile 
    1040                 1045                 1050             


Ile Phe  Lys Leu Asp Gly Asp  Ile Asp Arg Thr Gln  Phe Ile Trp 
    1055                 1060                 1065             


Met Lys  Tyr Phe Ser Ile Phe  Asn Thr Glu Leu Ser  Gln Ser Asn 
    1070                 1075                 1080             


Ile Glu  Glu Arg Tyr Lys Ile  Gln Ser Tyr Ser Glu  Tyr Leu Lys 
    1085                 1090                 1095             


Asp Phe  Trp Gly Asn Pro Leu  Met Tyr Asn Lys Glu  Tyr Tyr Met 
    1100                 1105                 1110             


Phe Asn  Ala Gly Asn Lys Asn  Ser Tyr Ile Lys Leu  Lys Lys Asp 
    1115                 1120                 1125             


Ser Pro  Val Gly Glu Ile Leu  Thr Arg Ser Lys Tyr  Asn Gln Asn 
    1130                 1135                 1140             


Ser Lys  Tyr Ile Asn Tyr Arg  Asp Leu Tyr Ile Gly  Glu Lys Phe 
    1145                 1150                 1155             


Ile Ile  Arg Arg Lys Ser Asn  Ser Gln Ser Ile Asn  Asp Asp Ile 
    1160                 1165                 1170             


Val Arg  Lys Glu Asp Tyr Ile  Tyr Leu Asp Phe Phe  Asn Leu Asn 
    1175                 1180                 1185             


Gln Glu  Trp Arg Val Tyr Thr  Tyr Lys Tyr Phe Lys  Lys Glu Glu 
    1190                 1195                 1200             


Met Lys  Leu Phe Leu Ala Pro  Ile Tyr Asp Ser Asp  Glu Phe Tyr 
    1205                 1210                 1215             


Asn Thr  Ile Gln Ile Lys Glu  Tyr Asp Glu Gln Pro  Thr Tyr Ser 
    1220                 1225                 1230             


Cys Gln  Leu Leu Phe Lys Lys  Asp Glu Glu Ser Thr  Asp Glu Ile 
    1235                 1240                 1245             


Gly Leu  Ile Gly Ile His Arg  Phe Tyr Glu Ser Gly  Ile Val Phe 
    1250                 1255                 1260             


Glu Glu  Tyr Lys Asp Tyr Phe  Cys Ile Ser Lys Trp  Tyr Leu Lys 
    1265                 1270                 1275             


Glu Val  Lys Arg Lys Pro Tyr  Asn Leu Lys Leu Gly  Cys Asn Trp 
    1280                 1285                 1290             


Gln Phe  Ile Pro Lys Asp Glu  Gly Trp Thr Glu 
    1295                 1300                 


<210>  65
<211>  1296
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide Sequence of mrBoNT/A(0)

<400>  65

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Gln 
    210                 215                 220                 


Leu Ile Tyr Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly Tyr Asn Lys 
        435                 440                 445             


Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe 
    450                 455                 460                 


Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu 
465                 470                 475                 480 


Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu 
                485                 490                 495     


Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro 
            500                 505                 510         


Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu 
        515                 520                 525             


Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu 
    530                 535                 540                 


Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu 
545                 550                 555                 560 


His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu 
                565                 570                 575     


Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys 
            580                 585                 590         


Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu 
        595                 600                 605             


Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr 
    610                 615                 620                 


Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala 
625                 630                 635                 640 


Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu 
                645                 650                 655     


Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala 
            660                 665                 670         


Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys 
        675                 680                 685             


Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu 
    690                 695                 700                 


Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys 
705                 710                 715                 720 


Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu 
                725                 730                 735     


Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn 
            740                 745                 750         


Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp 
        755                 760                 765             


Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile 
    770                 775                 780                 


Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met 
785                 790                 795                 800 


Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys 
                805                 810                 815     


Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly 
            820                 825                 830         


Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp 
        835                 840                 845             


Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser 
    850                 855                 860                 


Thr Phe Thr Glu Tyr Ile Lys Asn Ile Ile Asn Thr Ser Ile Leu Asn 
865                 870                 875                 880 


Leu Arg Tyr Glu Ser Lys His Leu Ile Asp Leu Ser Arg Tyr Ala Ser 
                885                 890                 895     


Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp Pro Ile Asp Lys Asn 
            900                 905                 910         


Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys Ile Glu Val Ile Leu 
        915                 920                 925             


Lys Lys Ala Ile Val Tyr Asn Ser Met Tyr Glu Asn Phe Ser Thr Ser 
    930                 935                 940                 


Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn Lys Ile Ser Leu Asn Asn 
945                 950                 955                 960 


Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn Ser Gly Trp Lys Val 
                965                 970                 975     


Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr Leu Gln Asp Thr Lys Glu 
            980                 985                 990         


Ile Lys Gln Arg Val Val Phe Lys  Tyr Ser Gln Met Ile  Asn Ile Ser 
        995                 1000                 1005             


Asp Tyr  Ile Asn Arg Trp Ile  Phe Val Thr Ile Thr  Asn Asn Arg 
    1010                 1015                 1020             


Leu Asn  Lys Ser Lys Ile Tyr  Ile Asn Gly Arg Leu  Ile Asp Gln 
    1025                 1030                 1035             


Lys Pro  Ile Ser Asn Leu Gly  Asn Ile His Ala Ser  Asn Lys Ile 
    1040                 1045                 1050             


Met Phe  Lys Leu Asp Gly Cys  Arg Asp Thr His Arg  Tyr Ile Trp 
    1055                 1060                 1065             


Ile Lys  Tyr Phe Asn Leu Phe  Asp Lys Glu Leu Asn  Glu Lys Glu 
    1070                 1075                 1080             


Ile Lys  Asp Leu Tyr Asp Asn  Gln Ser Asn Ser Gly  Ile Leu Lys 
    1085                 1090                 1095             


Asp Phe  Trp Gly Asp Tyr Leu  Gln Tyr Asp Lys Pro  Tyr Tyr Met 
    1100                 1105                 1110             


Leu Asn  Leu Tyr Asp Pro Asn  Lys Tyr Val Asp Val  Asn Asn Val 
    1115                 1120                 1125             


Gly Ile  Arg Gly Tyr Met Tyr  Leu Lys Gly Pro Arg  Gly Ser Val 
    1130                 1135                 1140             


Met Thr  Thr Asn Ile Tyr Leu  Asn Ser Ser Leu Tyr  Arg Gly Thr 
    1145                 1150                 1155             


Lys Phe  Ile Ile Lys Lys Tyr  Ala Ser Gly Asn Lys  Asp Asn Ile 
    1160                 1165                 1170             


Val Arg  Asn Asn Asp Arg Val  Tyr Ile Asn Val Val  Val Lys Asn 
    1175                 1180                 1185             


Lys Glu  Tyr Arg Leu Ala Thr  Asn Ala Ser Gln Ala  Gly Val Glu 
    1190                 1195                 1200             


Lys Ile  Leu Ser Ala Leu Glu  Ile Pro Asp Val Gly  Asn Leu Ser 
    1205                 1210                 1215             


Gln Val  Val Val Met Lys Ser  Lys Asn Asp Lys Gly  Ile Thr Asn 
    1220                 1225                 1230             


Lys Cys  Lys Met Asn Leu Gln  Asp Asn Asn Gly Asn  Asp Ile Gly 
    1235                 1240                 1245             


Phe Ile  Gly Phe His Gln Phe  Asn Asn Ile Ala Lys  Leu Val Ala 
    1250                 1255                 1260             


Ser Asn  Trp Tyr Asn Arg Gln  Ile Glu Arg Ser Ser  Arg Thr Leu 
    1265                 1270                 1275             


Gly Cys  Ser Trp Glu Phe Ile  Pro Val Asp Asp Gly  Trp Gly Glu 
    1280                 1285                 1290             


Arg Pro  Leu 
    1295     


<210>  66
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leucine-Based Motif


<220>
<221>  SITE
<222>  (1)..(1)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  SITE
<222>  (3)..(5)
<223>  Xaa can be any naturally occurring amino acid

<400>  66

Xaa Asp Xaa Xaa Xaa Leu Leu 
1               5           


<210>  67
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leucine-Based Motif


<220>
<221>  SITE
<222>  (1)..(1)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  SITE
<222>  (3)..(5)
<223>  Xaa can be any naturally occurring amino acid

<400>  67

Xaa Glu Xaa Xaa Xaa Leu Leu 
1               5           


<210>  68
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leucine-Based Motif


<220>
<221>  SITE
<222>  (1)..(1)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  SITE
<222>  (3)..(5)
<223>  Xaa can be any naturally occurring amino acid

<400>  68

Xaa Glu Xaa Xaa Xaa Ile Leu 
1               5           


<210>  69
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Leucine-Based Motif


<220>
<221>  SITE
<222>  (1)..(1)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  SITE
<222>  (3)..(5)
<223>  Xaa can be any naturally occurring amino acid

<400>  69

Xaa Glu Xaa Xaa Xaa Leu Met 
1               5           


<210>  70
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tyrosine-Based Motif


<220>
<221>  SITE
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  Xaa is a hydrophobic amino acid

<400>  70

Tyr Xaa Xaa Xaa 
1    


<210>  71
<211>  6
<212>  PRT
<213>  Clostridium botulinum

<400>  71

Asn Ile Ile Asn Thr Ser 
1               5       


<210>  72
<211>  6
<212>  PRT
<213>  Clostridium botulinum

<400>  72

Asn Ile Val Asn Thr Ser 
1               5       


<210>  73
<211>  6
<212>  PRT
<213>  Clostridium botulinum

<400>  73

Asn Ile Thr Asn Ala Ser 
1               5       


<210>  74
<211>  6
<212>  PRT
<213>  Clostridium botulinum

<400>  74

Asn Ile Thr Asn Thr Ser 
1               5       


<210>  75
<211>  6
<212>  PRT
<213>  Clostridium botulinum

<400>  75

Glu Ile Leu Asn Asn Ile 
1               5       


<210>  76
<211>  6
<212>  PRT
<213>  Clostridium botulinum

<400>  76

Asp Ile Leu Asn Asn Ile 
1               5       