                         LISTAGEM DE SEQUNCIAS

<110>  FUNDAO OSWALDO CRUZ
 
<120>  PROTENA QUIMRICA, KIT, MTODO PARA DIAGNSTICO DE LEISHMANIOSE, USO DE UMA PROTENA QUIMRICA, 
COMPOSIO VACINAL CONTRA LEISHMANIOSE VISCERAL, E, USO DE UMA COMPOSIO VACINAL 

<160>  2     

<170>  PatentIn version 3.5

<210>  1
<211>  402
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protena quimrica DTL4 por fuso das protenas A2 e K39 de 
       Leishmania

<400>  1

Met Lys Ile Arg Ser Val Arg Pro Leu Val Val Leu Leu Val Cys Val 
1               5                   10                  15      


Ala Ala Val Leu Ala Leu Ser Ala Ser Ala Glu Pro His Lys Ala Ala 
            20                  25                  30          


Val Asp Val Gly Pro Leu Ser Val Gly Pro Gln Ser Val Gly Pro Leu 
        35                  40                  45              


Ser Val Gly Pro Gln Ala Val Gly Pro Leu Ser Val Pro Gln Ser Val 
    50                  55                  60                  


Gly Pro Leu Ser Val Gly Pro Gln Ala Val Gly Pro Leu Ser Val Gly 
65                  70                  75                  80  


Pro Gln Ser Val Gly Pro Leu Ser Val Gly Pro Leu Ser Val Gly Pro 
                85                  90                  95      


Gln Ser Val Gly Pro Leu Ser Val Gly Ser Gln Ser Val Gly Pro Leu 
            100                 105                 110         


Ser Val Gly Pro Gln Ser Val Gly Pro Leu Ser Val Gly Pro Gln Ala 
        115                 120                 125             


Val Gly Pro Leu Ser Val Gly Pro Gln Ser Val Gly Pro Leu Ser Val 
    130                 135                 140                 


Gly Pro Gln Ala Val Gly Pro Leu Ser Val Gly Pro Gln Ser Val Gly 
145                 150                 155                 160 


Pro Leu Ser Val Gly Pro Gln Ser Val Gly Pro Leu Ser Val Gly Ser 
                165                 170                 175     


Gln Ser Val Gly Pro Leu Ser Val Gly Pro Gln Ser Val Gly Pro Leu 
            180                 185                 190         


Ser Val Gly Pro Gln Ser Val Gly Pro Leu Ser Val Gly Pro Gln Ser 
        195                 200                 205             


Val Gly Pro Leu Ser Val Gly Pro Gln Ser Val Gly Pro Leu Ser Val 
    210                 215                 220                 


Gly Pro Gln Ser Val Asp Val Ser Pro Val Ser Leu Glu Gln Leu Leu 
225                 230                 235                 240 


Arg Glu Ser Glu Glu Arg Ala Ala Glu Leu Ala Ser Gln Leu Glu Ser 
                245                 250                 255     


Thr Thr Ala Ala Lys Met Ser Ala Glu Gln Asp Glu Arg Glu Asn Thr 
            260                 265                 270         


Arg Ala Thr Leu Glu Gln Gln Leu Arg Asp Ser Glu Glu Arg Ala Ala 
        275                 280                 285             


Glu Leu Ala Ser Gln Leu Glu Ala Thr Ala Ala Ala Lys Ser Ser Ala 
    290                 295                 300                 


Glu Gln Asp Arg Glu Asn Thr Arg Ala Ala Leu Glu Gln Leu Leu Arg 
305                 310                 315                 320 


Glu Ser Glu Glu Arg Ala Ala Glu Leu Ala Ser Gln Leu Glu Ser Thr 
                325                 330                 335     


Thr Ala Ala Lys Met Ser Ala Glu Gln Asp Glu Arg Glu Asn Thr Arg 
            340                 345                 350         


Ala Thr Leu Glu Gln Gln Leu Arg Asp Ser Glu Glu Arg Ala Ala Glu 
        355                 360                 365             


Leu Ala Ser Gln Leu Glu Ala Thr Ala Ala Ala Lys Ser Ser Ala Glu 
    370                 375                 380                 


Gln Asp Arg Glu Asn Thr Arg Ala Ala Gly Gly Gly His His His His 
385                 390                 395                 400 


His His 
        

<210>  2
<211>  1212
<212>  DNA
<213>  Sequncia Artificial 

<220>
<223>  Gene sinttico codificante da DTL4 com tag de histidina

<400>  2
atgaagattc gttctgttcg tccattggtg gtgttgctgg tttgtgtggc cgccgttctg        60

gcactgtccg cgtccgctga gccgcataaa gcggctgttg atgtgggtcc gctgagcgtt       120

ggtccgcaaa gcgtgggtcc actgagcgtt ggtccacagg cggttggccc actgagcgtt       180

ggcccacaaa gcgtgggccc gctgagcgtt ggcccacagg cggttggccc gctgagcgtt       240

ggtccacaaa gcgtgggacc actgagcgtt ggtccgctga gcgtgggtcc gcagagcgtt       300

ggcccactga gcgtgggtag ccaaagcgtt ggcccgctga gcgtgggccc acagagcgtt       360

ggccctctga gcgtgggtcc gcaagcagtg ggcccgctga gcgtgggtcc acagagcgtt       420

ggccccctga gcgtgggccc acaagctgtc ggtccactga gcgtgggccc gcagagcgtt       480

ggcccgctga gcgtggggcc gcaaagcgtt ggtccactga gcgtgggtag ccagagcgtt       540

ggaccactga gcgtgggtcc acaaagcgtt ggacctctga gcgtgggccc acagagcgtt       600

gggccactga gcgtgggacc acaaagcgtt ggacccctga gcgttggtcc gcagagcgtt       660

ggtccactga gcgttggccc gcaaagcgtt gatgtgagcc cggttagcct ggaacagctg       720

ctgcgtgaaa gcgaagaacg tgcagcggaa ctggcgagcc agctggaaag caccaccgct       780

gcaaaaatga gcgcagaaca agatgaacgt gaaaataccc gtgcgaccct ggaacaacag       840

ctgcgtgaca gcgaagaacg tgcggctgaa ctggctagcc aactggaagc aaccgcagcg       900

gctaaaagca gcgcggaaca ggaccgtgaa aacacccgtg cagcgctgga acaattgttg       960

agagagagcg aagaacgtgc tgcagaactg gcttctcaac tggaaagcac caccgcggct      1020

aagatgagcg ctgagcagga cgagcgtgaa aatacccgtg caaccctgga acagcaattg      1080

agagactctg aggagagagc tgctgaactg gcgagccaac tggaagctac cgctgcagcg      1140

aagagcagcg cggagcagga cagagaaaac accagagcag cgggcggcgg tcatcatcat      1200

caccatcact aa                                                          1212

