                         SEQUENCE LISTING

<110>  Dharmacon, Inc.
       Horizon Discovery Ltd.
 
<120>  Guide RNA Designs and Complexes for Type V Cas Systems

<130>  HORIZON 0109-WO

<150>  US 63/133,945
<151>  2021-01-05

<160>  97    

<170>  PatentIn version 3.5

<210>  1
<211>  56
<212>  RNA
<213>  Alicyclobacillus acidoterrestris


<220>
<221>  misc_feature
<222>  (37)..(46)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (47)..(56)
<223>  N is A, C, G, or U

<400>  1
gucggaucac ugagcgagcg aucugagaag uggcacnnnn nnnnnnnnnn nnnnnn           56


<210>  2
<211>  42
<212>  RNA
<213>  Alicyclobacillus acidoterrestris


<220>
<221>  misc_feature
<222>  (23)..(42)
<223>  N is A, C, G, or U

<400>  2
cgagcgaucu gagaaguggc acnnnnnnnn nnnnnnnnnn nn                          42


<210>  3
<211>  34
<212>  RNA
<213>  Alicyclobacillus acidoterrestris


<220>
<221>  misc_feature
<222>  (14)..(34)
<223>  N is A, C, G, or U

<400>  3
cugagaagug gcacnnnnnn nnnnnnnnnn nnnn                                   34


<210>  4
<211>  79
<212>  RNA
<213>  Alicyclobacillus acidoterrestris

<400>  4
gucuagagga cagaauuuuu caacgggugu gccaauggcc acuuuccagg uggcaaagcc       60

cguugagcuu cucaaaaag                                                    79


<210>  5
<211>  78
<212>  RNA
<213>  Alicyclobacillus acidoterrestris

<400>  5
gucuagagga cagaauuuuu caacgggugu gccaauggcc acuuuccagg uggcaaagcc       60

cguugagcuu cucaaaaa                                                     78


<210>  6
<211>  67
<212>  RNA
<213>  Alicyclobacillus acidoterrestris

<400>  6
gucuagagga cagaauuuuu caacgggugu gccaauggcc acuuuccagg uggcaaagcc       60

cguugag                                                                 67


<210>  7
<211>  111
<212>  RNA
<213>  Alicyclobacillus acidoterrestris


<220>
<221>  misc_feature
<222>  (92)..(111)
<223>  N is A, C, G, or U

<400>  7
gucuagagga cagaauuuuu caacgggugu gccaauggcc acuuuccagg uggcaaagcc       60

cguugagcuu cucaaaucug agaaguggca cnnnnnnnnn nnnnnnnnnn n               111


<210>  8
<211>  104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (85)..(104)
<223>  N is A, C, G, or U

<400>  8
gucgucuaua ggacggcgag gacaacggga gugcagugcu cuuuccaaga gcaaacaccc       60

cguuggcuuc aagagaagug gcacnnnnnn nnnnnnnnnn nnnn                       104


<210>  9
<211>  91
<212>  RNA
<213>  Bacillus thermoamylovorans

<400>  9
cgagguucug ucuuuugguc aggacaaccg ucuagcuaua agugcugcag gggugugaga       60

aacuccuauu gcuggacgau gucucuuuua u                                      91


<210>  10
<211>  56
<212>  RNA
<213>  Bacillus thermoamylovorans


<220>
<221>  misc_feature
<222>  (37)..(56)
<223>  N is A, C, G, or U

<400>  10
guccaagaaa aaagaaauga uacgaggcau uagcacnnnn nnnnnnnnnn nnnnnn           56


<210>  11
<211>  42
<212>  RNA
<213>  Bacillus thermoamylovorans


<220>
<221>  misc_feature
<222>  (23)..(42)
<223>  N is A, C, G, or U

<400>  11
aaaugauacg aggcauuagc acnnnnnnnn nnnnnnnnnn nn                          42


<210>  12
<211>  34
<212>  RNA
<213>  Bacillus thermoamylovorans


<220>
<221>  misc_feature
<222>  (15)..(34)
<223>  N is A, C, G, or U

<400>  12
cgaggcauua gcacnnnnnn nnnnnnnnnn nnnn                                   34


<210>  13
<211>  44
<212>  RNA
<213>  Unknown

<220>
<223>  Deltaproteobacteria


<220>
<221>  misc_feature
<222>  (24)..(44)
<223>  N is A, C, G, or U

<400>  13
ccgauaagua aaacgcauca aagnnnnnnn nnnnnnnnnn nnnn                        44


<210>  14
<211>  75
<212>  RNA
<213>  Unknown

<220>
<223>  Deltaproteobacteria

<400>  14
ggcgcguuua uuccauuacu uuggagccag ucccagcgac uaugucguau ggacgaagcg       60

cuuauuuauc ggaga                                                        75


<210>  15
<211>  122
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (102)..(122)
<223>  N is A, C, G, or U

<400>  15
ggcgcguuua uuccauuacu uuggagccag ucccagcgac uaugucguau ggacgaagcg       60

cuuauuuauc ggagagaaac cgauaaguaa aacgcaucaa annnnnnnnn nnnnnnnnnn      120

nn                                                                     122


<210>  16
<211>  128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (109)..(128)
<223>  N is A, C, G, or U

<400>  16
acaucuggcg cguuuauucc auuacuuugg agccaguccc agcgacuaug ucguauggac       60

gaagcgcuua uuuaucggag agaaaccgau aaguaaaacg caucaaagnn nnnnnnnnnn      120

nnnnnnnn                                                               128


<210>  17
<211>  225
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (206)..(225)
<223>  N is A, C, G, or U

<400>  17
gggcuucacu gauaaagugg agaaccgcuu caccaaaagc ugucccuuag gggauuagaa       60

cuugagugaa ggugggcugc uugcaucagc cuaaugucga gaagugcuuu cuucggaaag      120

uaacccucga aacaaauuca uuuuuccucu ccaauucugc acaagaaagu ugcagaaccc      180

gaauagacga augaaggaau gcaacnnnnn nnnnnnnnnn nnnnn                      225


<210>  18
<211>  116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  18
cuucaccaaa agcugucccu uaggggauua gaacuugagu gaaggugggc ugcuugcauc       60

agccuaaugu cgagaagugc uuucuucgga aaguaacccu cgaaacaaau ucauuu          116


<210>  19
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (18)..(37)
<223>  N is A, C, G, or U

<400>  19
gaaugaagga augcaacnnn nnnnnnnnnn nnnnnnn                                37


<210>  20
<211>  57
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (38)..(57)
<223>  N is A, C, G, or U

<400>  20
guugcagaac ccgaauagac gaaugaagga augcaacnnn nnnnnnnnnn nnnnnnn          57


<210>  21
<211>  157
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (138)..(157)
<223>  n is a, c, g, or u

<400>  21
cuucaccaaa agcugucccu uaggggauua gaacuugagu gaaggugggc ugcuugcauc       60

agccuaaugu cgagaagugc uuucuucgga aaguaacccu cgaaacaaau ucauuugaaa      120

gaaugaagga augcaacnnn nnnnnnnnnn nnnnnnn                               157


<210>  22
<211>  84
<212>  PRT
<213>  Unknown

<220>
<223>  Bacillus phage PBS2

<400>  22

Met Thr Asn Leu Ser Asp Ile Ile Glu Lys Glu Thr Gly Lys Gln Leu 
1               5                   10                  15      


Val Ile Gln Glu Ser Ile Leu Met Leu Pro Glu Glu Val Glu Glu Val 
            20                  25                  30          


Ile Gly Asn Lys Pro Glu Ser Asp Ile Leu Val His Thr Ala Tyr Asp 
        35                  40                  45              


Glu Ser Thr Asp Glu Asn Val Met Leu Leu Thr Ser Asp Ala Pro Glu 
    50                  55                  60                  


Tyr Lys Pro Trp Ala Leu Val Ile Gln Asp Ser Asn Gly Glu Asn Lys 
65                  70                  75                  80  


Ile Lys Met Leu 
                


<210>  23
<211>  66
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Telomerase Ku biding motif

<400>  23
uucuugucgu acuuauagau cgcuacguua uuucaauuuu gaaaaucuga guccugggag       60

ugcgga                                                                  66


<210>  24
<211>  608
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ku heterodimer

<400>  24

Met Ser Gly Trp Glu Ser Tyr Tyr Lys Thr Glu Gly Asp Glu Glu Ala 
1               5                   10                  15      


Glu Glu Glu Gln Glu Glu Asn Leu Glu Ala Ser Gly Asp Tyr Lys Tyr 
            20                  25                  30          


Ser Gly Arg Asp Ser Leu Ile Phe Leu Val Asp Ala Ser Lys Ala Met 
        35                  40                  45              


Phe Glu Ser Gln Ser Glu Asp Glu Leu Thr Pro Phe Asp Met Ser Ile 
    50                  55                  60                  


Gln Cys Ile Gln Ser Val Tyr Ile Ser Lys Ile Ile Ser Ser Asp Arg 
65                  70                  75                  80  


Asp Leu Leu Ala Val Val Phe Tyr Gly Thr Glu Lys Asp Lys Asn Ser 
                85                  90                  95      


Val Asn Phe Lys Asn Ile Tyr Val Leu Gln Glu Leu Asp Asn Pro Gly 
            100                 105                 110         


Ala Lys Arg Ile Leu Glu Leu Asp Gln Phe Lys Gly Gln Gln Gly Gln 
        115                 120                 125             


Lys Arg Phe Gln Asp Met Met Gly His Gly Ser Asp Tyr Ser Leu Ser 
    130                 135                 140                 


Glu Val Leu Trp Val Cys Ala Asn Leu Phe Ser Asp Val Gln Phe Lys 
145                 150                 155                 160 


Met Ser His Lys Arg Ile Met Leu Phe Thr Asn Glu Asp Asn Pro His 
                165                 170                 175     


Gly Asn Asp Ser Ala Lys Ala Ser Arg Ala Arg Thr Lys Ala Gly Asp 
            180                 185                 190         


Leu Arg Asp Thr Gly Ile Phe Leu Asp Leu Met His Leu Lys Lys Pro 
        195                 200                 205             


Gly Gly Phe Asp Ile Ser Leu Phe Tyr Arg Asp Ile Ile Ser Ile Ala 
    210                 215                 220                 


Glu Asp Glu Asp Leu Arg Val His Phe Glu Glu Ser Ser Lys Leu Glu 
225                 230                 235                 240 


Asp Leu Leu Arg Lys Val Arg Ala Lys Glu Thr Arg Lys Arg Ala Leu 
                245                 250                 255     


Ser Arg Leu Lys Leu Lys Leu Asn Lys Asp Ile Val Ile Ser Val Gly 
            260                 265                 270         


Ile Tyr Asn Leu Val Gln Lys Ala Leu Lys Pro Pro Pro Ile Lys Leu 
        275                 280                 285             


Tyr Arg Glu Thr Asn Glu Pro Val Lys Thr Lys Thr Arg Thr Phe Asn 
    290                 295                 300                 


Thr Ser Thr Gly Gly Leu Leu Leu Pro Ser Asp Thr Lys Arg Ser Gln 
305                 310                 315                 320 


Ile Tyr Gly Ser Arg Gln Ile Ile Leu Glu Lys Glu Glu Thr Glu Glu 
                325                 330                 335     


Leu Lys Arg Phe Asp Asp Pro Gly Leu Met Leu Met Gly Phe Lys Pro 
            340                 345                 350         


Leu Val Leu Leu Lys Lys His His Tyr Leu Arg Pro Ser Leu Phe Val 
        355                 360                 365             


Tyr Pro Glu Glu Ser Leu Val Ile Gly Ser Ser Thr Leu Phe Ser Ala 
    370                 375                 380                 


Leu Leu Ile Lys Cys Leu Glu Lys Glu Val Ala Ala Leu Cys Arg Tyr 
385                 390                 395                 400 


Thr Pro Arg Arg Asn Ile Pro Pro Tyr Phe Val Ala Leu Val Pro Gln 
                405                 410                 415     


Glu Glu Glu Leu Asp Asp Gln Lys Ile Gln Val Thr Pro Pro Gly Phe 
            420                 425                 430         


Gln Leu Val Phe Leu Pro Phe Ala Asp Asp Lys Arg Lys Met Pro Phe 
        435                 440                 445             


Thr Glu Lys Ile Met Ala Thr Pro Glu Gln Val Gly Lys Met Lys Ala 
    450                 455                 460                 


Ile Val Glu Lys Leu Arg Phe Thr Tyr Arg Ser Asp Ser Phe Glu Asn 
465                 470                 475                 480 


Pro Val Leu Gln Gln His Phe Arg Asn Leu Glu Ala Leu Ala Leu Asp 
                485                 490                 495     


Leu Met Glu Pro Glu Gln Ala Val Asp Leu Thr Leu Pro Lys Val Glu 
            500                 505                 510         


Ala Met Asn Lys Arg Leu Gly Ser Leu Val Asp Glu Phe Lys Glu Leu 
        515                 520                 525             


Val Tyr Pro Pro Asp Tyr Asn Pro Glu Gly Lys Val Thr Lys Arg Lys 
    530                 535                 540                 


His Asp Asn Glu Gly Ser Gly Ser Lys Arg Pro Lys Val Glu Tyr Ser 
545                 550                 555                 560 


Glu Glu Glu Leu Lys Thr His Ile Ser Lys Gly Thr Leu Gly Lys Phe 
                565                 570                 575     


Thr Val Pro Met Leu Lys Glu Ala Cys Arg Ala Tyr Gly Leu Lys Ser 
            580                 585                 590         


Gly Leu Lys Lys Gln Glu Leu Leu Glu Ala Leu Thr Lys His Phe Gln 
        595                 600                 605             


<210>  25
<211>  485
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ku heterodimer

<400>  25

Met Val Arg Ser Gly Asn Lys Ala Ala Val Val Leu Cys Met Asp Val 
1               5                   10                  15      


Gly Phe Thr Met Ser Asn Ser Ile Pro Gly Ile Glu Ser Pro Phe Glu 
            20                  25                  30          


Gln Ala Lys Lys Val Ile Thr Met Phe Val Gln Arg Gln Val Phe Ala 
        35                  40                  45              


Glu Asn Lys Asp Glu Ile Ala Leu Val Leu Phe Gly Thr Asp Gly Thr 
    50                  55                  60                  


Asp Asn Pro Leu Ser Gly Gly Asp Gln Tyr Gln Asn Ile Thr Val His 
65                  70                  75                  80  


Arg His Leu Met Leu Pro Asp Phe Asp Leu Leu Glu Asp Ile Glu Ser 
                85                  90                  95      


Lys Ile Gln Pro Gly Ser Gln Gln Ala Asp Phe Leu Asp Ala Leu Ile 
            100                 105                 110         


Val Ser Met Asp Val Ile Gln His Glu Thr Ile Gly Lys Lys Phe Glu 
        115                 120                 125             


Lys Arg His Ile Glu Ile Phe Thr Asp Leu Ser Ser Arg Phe Ser Lys 
    130                 135                 140                 


Ser Gln Leu Asp Ile Ile Ile His Ser Leu Lys Lys Cys Asp Ile Ser 
145                 150                 155                 160 


Glu Arg His Ser Ile His Trp Pro Cys Arg Leu Thr Ile Gly Ser Asn 
                165                 170                 175     


Leu Ser Ile Arg Ile Ala Ala Tyr Lys Ser Ile Leu Gln Glu Arg Val 
            180                 185                 190         


Lys Lys Thr Trp Thr Val Val Asp Ala Lys Thr Leu Lys Lys Glu Asp 
        195                 200                 205             


Ile Gln Lys Glu Thr Val Tyr Cys Leu Asn Asp Asp Asp Glu Thr Glu 
    210                 215                 220                 


Val Leu Lys Glu Asp Ile Ile Gln Gly Phe Arg Tyr Gly Ser Asp Ile 
225                 230                 235                 240 


Val Pro Phe Ser Lys Val Asp Glu Glu Gln Met Lys Tyr Lys Ser Glu 
                245                 250                 255     


Gly Lys Cys Phe Ser Val Leu Gly Phe Cys Lys Ser Ser Gln Val Gln 
            260                 265                 270         


Arg Arg Phe Phe Met Gly Asn Gln Val Leu Lys Val Phe Ala Ala Arg 
        275                 280                 285             


Asp Asp Glu Ala Ala Ala Val Ala Leu Ser Ser Leu Ile His Ala Leu 
    290                 295                 300                 


Asp Asp Leu Asp Met Val Ala Ile Val Arg Tyr Ala Tyr Asp Lys Arg 
305                 310                 315                 320 


Ala Asn Pro Gln Val Gly Val Ala Phe Pro His Ile Lys His Asn Tyr 
                325                 330                 335     


Glu Cys Leu Val Tyr Val Gln Leu Pro Phe Met Glu Asp Leu Arg Gln 
            340                 345                 350         


Tyr Met Phe Ser Ser Leu Lys Asn Ser Lys Lys Tyr Ala Pro Thr Glu 
        355                 360                 365             


Ala Gln Leu Asn Ala Val Asp Ala Leu Ile Asp Ser Met Ser Leu Ala 
    370                 375                 380                 


Lys Lys Asp Glu Lys Thr Asp Thr Leu Glu Asp Leu Phe Pro Thr Thr 
385                 390                 395                 400 


Lys Ile Pro Asn Pro Arg Phe Gln Arg Leu Phe Gln Cys Leu Leu His 
                405                 410                 415     


Arg Ala Leu His Pro Arg Glu Pro Leu Pro Pro Ile Gln Gln His Ile 
            420                 425                 430         


Trp Asn Met Leu Asn Pro Pro Ala Glu Val Thr Thr Lys Ser Gln Ile 
        435                 440                 445             


Pro Leu Ser Lys Ile Lys Thr Leu Phe Pro Leu Ile Glu Ala Lys Lys 
    450                 455                 460                 


Lys Asp Gln Val Thr Ala Gln Glu Ile Phe Gln Asp Asn His Glu Asp 
465                 470                 475                 480 


Gly Pro Thr Ala Lys 
                485 


<210>  26
<211>  10
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Telomerase Sm7 binding motif

<400>  26
aauuuuugga                                                              10


<210>  27
<211>  83
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sm consensus site

<400>  27

Gly Ser Val Ile Asp Val Ser Ser Gln Arg Val Asn Val Gln Arg Pro 
1               5                   10                  15      


Leu Asp Ala Leu Gly Asn Ser Leu Asn Ser Pro Val Ile Ile Lys Leu 
            20                  25                  30          


Lys Gly Asp Arg Glu Phe Arg Gly Val Leu Lys Ser Phe Asp Leu His 
        35                  40                  45              


Met Asn Leu Val Leu Asn Asp Ala Glu Glu Leu Glu Asp Gly Glu Val 
    50                  55                  60                  


Thr Arg Arg Leu Gly Thr Val Leu Ile Arg Gly Asp Asn Ile Val Tyr 
65                  70                  75                  80  


Ile Ser Pro 
            


<210>  28
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  MS2 phage operator stem loop

<400>  28
gcacaugagg aucacccaug ugc                                               23


<210>  29
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MS2 coat protein

<400>  29

Met Ala Ser Asn Phe Thr Gln Phe Val Leu Val Asp Asn Gly Gly Thr 
1               5                   10                  15      


Gly Asp Val Thr Val Ala Pro Ser Asn Phe Ala Asn Gly Ile Ala Glu 
            20                  25                  30          


Trp Ile Ser Ser Asn Ser Arg Ser Gln Ala Tyr Lys Val Thr Cys Ser 
        35                  40                  45              


Val Arg Gln Ser Ser Ala Gln Asn Arg Lys Tyr Thr Ile Lys Val Glu 
    50                  55                  60                  


Val Pro Lys Gly Ala Trp Arg Ser Tyr Leu Asn Met Glu Leu Thr Ile 
65                  70                  75                  80  


Pro Ile Phe Ala Thr Asn Ser Asp Cys Glu Leu Ile Val Lys Ala Met 
                85                  90                  95      


Gln Gly Leu Leu Lys Asp Gly Asn Pro Ile Pro Ser Ala Ile Ala Ala 
            100                 105                 110         


Asn Ser Gly Ile Tyr 
        115         


<210>  30
<211>  26
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  PP7 hage operator stem loop

<400>  30
auaaggaguu uauauggaaa cccuua                                            26


<210>  31
<211>  128
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PP7 coat protein PCP

<400>  31

Met Ser Lys Thr Ile Val Leu Ser Val Gly Glu Ala Thr Arg Thr Leu 
1               5                   10                  15      


Thr Glu Ile Gln Ser Thr Ala Asp Arg Gln Ile Phe Glu Glu Lys Val 
            20                  25                  30          


Gly Pro Leu Val Gly Arg Leu Arg Leu Thr Ala Ser Leu Arg Gln Asn 
        35                  40                  45              


Gly Ala Lys Thr Ala Tyr Arg Val Asn Leu Lys Leu Asp Gln Ala Asp 
    50                  55                  60                  


Val Val Asp Cys Ser Thr Ser Val Cys Gly Glu Leu Pro Lys Val Arg 
65                  70                  75                  80  


Tyr Thr Gln Val Trp Ser His Asp Val Thr Ile Val Ala Asn Ser Thr 
                85                  90                  95      


Glu Ala Ser Arg Lys Ser Leu Tyr Asp Leu Thr Lys Ser Leu Val Ala 
            100                 105                 110         


Thr Ser Gln Val Glu Asp Leu Val Val Asn Leu Val Pro Leu Gly Arg 
        115                 120                 125             


<210>  32
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  SfMu Com Stem Loop

<400>  32
cugaaugccu gcgagcauc                                                    19


<210>  33
<211>  62
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SfMU Com binding protein

<400>  33

Met Lys Ser Ile Arg Cys Lys Asn Cys Asn Lys Leu Leu Phe Lys Ala 
1               5                   10                  15      


Asp Ser Phe Asp His Ile Glu Ile Arg Cys Pro Arg Cys Lys Arg His 
            20                  25                  30          


Ile Ile Met Leu Asn Ala Cys Glu His Pro Thr Glu Lys His Cys Gly 
        35                  40                  45              


Lys Arg Glu Lys Ile Thr His Ser Asp Glu Thr Val Arg Tyr 
    50                  55                  60          


<210>  34
<211>  15
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Box B Aptamer

<400>  34
gcccugaaga agggc                                                        15


<210>  35
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Lambda N22plus protein

<400>  35

Met Asn Ala Arg Thr Arg Arg Arg Glu Arg Arg Ala Glu Lys Gln Ala 
1               5                   10                  15      


Gln Trp Lys Ala Ala Asn 
            20          


<210>  36
<211>  16
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Csy4 binding motif

<400>  36
cugccguaua ggcagc                                                       16


<210>  37
<211>  187
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Csy4[H29A]

<400>  37

Met Asp His Tyr Leu Asp Ile Arg Leu Arg Pro Asp Pro Glu Phe Pro 
1               5                   10                  15      


Pro Ala Gln Leu Met Ser Val Leu Phe Gly Lys Leu Ala Gln Ala Leu 
            20                  25                  30          


Val Ala Gln Gly Gly Asp Arg Ile Gly Val Ser Phe Pro Asp Leu Asp 
        35                  40                  45              


Glu Ser Arg Ser Arg Leu Gly Glu Arg Leu Arg Ile His Ala Ser Ala 
    50                  55                  60                  


Asp Asp Leu Arg Ala Leu Leu Ala Arg Pro Trp Leu Glu Gly Leu Arg 
65                  70                  75                  80  


Asp His Leu Gln Phe Gly Glu Pro Ala Val Val Pro His Pro Thr Pro 
                85                  90                  95      


Tyr Arg Gln Val Ser Arg Val Gln Ala Lys Ser Asn Pro Glu Arg Leu 
            100                 105                 110         


Arg Arg Arg Leu Met Arg Arg His Asp Leu Ser Glu Glu Glu Ala Arg 
        115                 120                 125             


Lys Arg Ile Pro Asp Thr Val Ala Arg Ala Leu Asp Leu Pro Phe Val 
    130                 135                 140                 


Thr Leu Arg Ser Gln Ser Thr Gly Gln His Phe Arg Leu Phe Ile Arg 
145                 150                 155                 160 


His Gly Pro Leu Gln Val Thr Ala Glu Glu Gly Gly Phe Thr Cys Tyr 
                165                 170                 175     


Gly Leu Ser Lys Gly Gly Phe Val Pro Trp Phe 
            180                 185         


<210>  38
<211>  147
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (127)..(147)
<223>  N is A, C, G, T, or U

<400>  38
gcgcacatga ggatcaccca tgtgcggcgc guuuauucca uuacuuugga gccaguccca       60

gcgacuaugu cguauggacg aagcgcuuau uuaucggaga gaaaccgaua aguaaaacgc      120

aucaaannnn nnnnnnnnnn nnnnnnn                                          147


<210>  39
<211>  146
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (126)..(146)
<223>  N is A, C, G, T, or U

<400>  39
ggcgcguuua uuccauuacu uuggagccag ucccagcgac ugcgcacatg aggatcaccc       60

atgtgcuguc guauggacga agcgcuuauu uaucggagag aaaccgauaa guaaaacgca      120

ucaaannnnn nnnnnnnnnn nnnnnn                                           146


<210>  40
<211>  146
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (126)..(146)
<223>  N is A, C, G, or U

<400>  40
ggcgcguuua uuccauuacu uuggagccag ucccagcgac uaugucguau ggacgaagcg       60

cuuauuuauc ggagagcgca catgaggatc acccatgtgc aaaccgauaa guaaaacgca      120

ucaaannnnn nnnnnnnnnn nnnnnn                                           146


<210>  41
<211>  147
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (102)..(122)
<223>  N is A, C, G, T, or U

<400>  41
ggcgcguuua uuccauuacu uuggagccag ucccagcgac uaugucguau ggacgaagcg       60

cuuauuuauc ggagagaaac cgauaaguaa aacgcaucaa annnnnnnnn nnnnnnnnnn      120

nngcgcacat gaggatcacc catgtgc                                          147


<210>  42
<211>  171
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (151)..(171)
<223>  N is A, C., G, T,  or U

<400>  42
gcgcacatga ggatcaccca tgtgcggcgc guuuauucca uuacuuugga gccaguccca       60

gcgacuaugu cguauggacg aagcgcuuau uuaucggaga gcgcacatga ggatcaccca      120

tgtgcaaacc gauaaguaaa acgcaucaaa nnnnnnnnnn nnnnnnnnnn n               171


<210>  43
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  43
agaaccggag gacaaagtac                                                   20


<210>  44
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  44
tggcaatgcg ccaccggttg                                                   20


<210>  45
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  45
tcttctgctc ggactcaggc                                                   20


<210>  46
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  46
tctgctcgga ctcaggccct                                                   20


<210>  47
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  47
ccagcttctg ccgtttgtac                                                   20


<210>  48
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  48
aaaaacagtg gataattttg                                                   20


<210>  49
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  49
gaagagacca aagatcaccc                                                   20


<210>  50
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  50
cctccgcctg tggatgctgc                                                   20


<210>  51
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  51
tcctgctgct gccgggacct                                                   20


<210>  52
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  52
gcggccgatg agaagaagaa                                                   20


<210>  53
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (49)..(69)
<223>  N is A, C, G, T, or U

<400>  53
gcgcacatga ggatcaccca tgtgcccgau aaguaaaacg caucaaagnn nnnnnnnnnn       60

nnnnnnnnn                                                               69


<210>  54
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (24)..(44)
<223>  N is A, C, G, T, or U

<400>  54
ccgauaagua aaacgcauca aagnnnnnnn nnnnnnnnnn nnnngcgcac atgaggatca       60

cccatgtgc                                                               69


<210>  55
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  55
gcgcacatga ggatcaccca tgtgcggcgc guuuauucca uuacuuugga gccaguccca       60

gcgacuaugu cguauggacg aagcgcuuau uuaucggaga                            100


<210>  56
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  56
ggcgcguuua uuccauuacu uuggagccag ucccagcgac ugcgcacatg aggatcaccc       60

atgtgcuguc guauggacga agcgcuuauu uaucggaga                              99


<210>  57
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  57
ggcgcguuua uuccauuacu uuggagccag ucccagcgac uaugucguau ggacgaagcg       60

cuuauuuauc ggagagcgca catgaggatc acccatgtgc                            100


<210>  58
<211>  118
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (92)..(111)
<223>  N is A, C, G, U, or T

<400>  58
gtctagagga cagaattttt caacgggtgt gccaatggcc actttccagg tggcaaagcc       60

cgttgagctt ctcaaatctg agaagtggca cnnnnnnnnn nnnnnnnnnn nttttttt        118


<210>  59
<211>  142
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (116)..(135)
<223>  N is A, C, G, or T

<400>  59
ggcacatgag gatcacccat gtgcgtctag aggacagaat ttttcaacgg gtgtgccaat       60

ggccactttc caggtggcaa agcccgttga gcttctcaaa tctgagaagt ggcacnnnnn      120

nnnnnnnnnn nnnnnttttt tt                                               142


<210>  60
<211>  142
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (116)..(135)
<223>  N is A, C, G, or T

<400>  60
ggcacatgag gatcacccat gtgcgtctag aggacagaat tgttcaacgg gtgtgccaat       60

ggccactttc caggtggcaa agcccgttga gcttctcaaa tctgagaagt ggcacnnnnn      120

nnnnnnnnnn nnnnnttttt tt                                               142


<210>  61
<211>  142
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (116)..(135)
<223>  N is A, C, G, or T

<400>  61
ggtctagagg acagaatttt tcaacgggtg tgccaatggc cactttccag gtggcaaagc       60

ccgttgagct tctcaaatct gagaagtggc acgcacatga ggatcaccca tgtgcnnnnn      120

nnnnnnnnnn nnnnnttttt tt                                               142


<210>  62
<211>  142
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (116)..(135)
<223>  N is A, C, G, or T

<400>  62
ggtctagagg acagaattgt tcaacgggtg tgccaatggc cactttccag gtggcaaagc       60

ccgttgagct tctcaaatct gagaagtggc acgcacatga ggatcaccca tgtgcnnnnn      120

nnnnnnnnnn nnnnnttttt tt                                               142


<210>  63
<211>  131
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (105)..(124)
<223>  N is A, C, G, or T

<400>  63
ggtctagagg acagaatttt tcaacgggtg tgccaatgga catgaggatc acccatgtcc       60

aggtggcaaa gcccgttgag cttctcaaat ctgagaagtg gcacnnnnnn nnnnnnnnnn      120

nnnntttttt t                                                           131


<210>  64
<211>  131
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (105)..(124)
<223>  N is A, C, G, or T

<400>  64
ggtctagagg acagaattgt tcaacgggtg tgccaatgga catgaggatc acccatgtcc       60

aggtggcaaa gcccgttgag cttctcaaat ctgagaagtg gcacnnnnnn nnnnnnnnnn      120

nnnntttttt t                                                           131


<210>  65
<211>  142
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (116)..(135)
<223>  N is A, C, G, or T

<400>  65
ggtctagagg acagaattgc acatgaggat cacccatgtg ctttcaacgg gtgtgccaat       60

ggccactttc caggtggcaa agcccgttga gcttctcaaa tctgagaagt ggcacnnnnn      120

nnnnnnnnnn nnnnnttttt tt                                               142


<210>  66
<211>  192
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (163)..(185)
<223>  N is A, C, G, or T

<400>  66
atgggcttca ctgataaagt ggagaaccgc ttcaccaaaa gctgtccctt aggggattag       60

aacttgagtg aaggtgggct gcttgcatca gcctaatgtc gagaagtgct ttcttcggaa      120

agtaaccctc gaaacaaatt catttgaatg aaggaatgca acnnnnnnnn nnnnnnnnnn      180

nnnnnttttt tt                                                          192


<210>  67
<211>  215
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (186)..(208)
<223>  N is A, C, G, or T

<400>  67
gcacatgagg atcacccatg tgcatgggct tcactgataa agtggagaac cgcttcacca       60

aaagctgtcc cttaggggat tagaacttga gtgaaggtgg gctgcttgca tcagcctaat      120

gtcgagaagt gctttcttcg gaaagtaacc ctcgaaacaa attcatttga atgaaggaat      180

gcaacnnnnn nnnnnnnnnn nnnnnnnntt ttttt                                 215


<210>  68
<211>  188
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (159)..(181)
<223>  N is A, C, G, or T

<400>  68
gcacatgagg atcacccatg tgccgcttca ccaaaagctg tcccttaggg gattagaact       60

tgagtgaagg tgggctgctt gcatcagcct aatgtcgaga agtgctttct tcggaaagta      120

accctcgaaa caaattcatt tgaatgaagg aatgcaacnn nnnnnnnnnn nnnnnnnnnn      180

nttttttt                                                               188


<210>  69
<211>  158
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (129)..(151)
<223>  N is A, C, G, or T

<400>  69
accgcttcac gcacatgagg atcacccatg tgcgtgaagg tgggctgctt gcatcagcct       60

aatgtcgaga agtgctttct tcggaaagta accctcgaaa caaattcatt tgaatgaagg      120

aatgcaacnn nnnnnnnnnn nnnnnnnnnn nttttttt                              158


<210>  70
<211>  158
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (129)..(151)
<223>  N is A, C, G, or T

<400>  70
accgcttcac gcacatgagg atcacccatg tgcgtgaagg tgggctgctt gcatcagcct       60

aatgtcgaga agtgctttct tcggaaagta accctcgaaa caaattcatt tgaatgaagg      120

aatgcaacnn nnnnnnnnnn nnnnnnnnnn nttttttt                              158


<210>  71
<211>  199
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid containing sequences from Cas12b, SV40 NLS


<220>
<221>  misc_feature
<222>  (170)..(192)
<223>  N is A, C, G, or T

<400>  71
gggcttcact gataaagtgg agaaccgctt caccaaaagc tgtcccttag gggattagaa       60

cttgagtgaa ggtgggctgc ttgcatcagc ctaatgtcga gaagtgcttt cttcggaaag      120

taaccctcga aacaaagcac atgaggatca cccatgtgcg gaatgcaacn nnnnnnnnnn      180

nnnnnnnnnn nnttttttt                                                   199


<210>  72
<211>  4035
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid containing sequences from humanized Cas12b and SV40 NLS

<400>  72
atggccccaa agaagaagcg gaaagtcgcc gtgaaaagca ttaaagtgaa actgaggctg       60

gacgatatgc ccgaaatcag agccggcctc tggaagctgc acaaggaggt caacgccggc      120

gttcgttatt acacagagtg gctgtcttta ctcagacaag aaaatttata tagaaggagc      180

cccaatggcg atggcgagca agagtgcgac aaaaccgccg aagaatgcaa ggccgaactg      240

ctcgaaagac tgagagccag acaagttgag aacggacaca gaggacccgc cggctccgat      300

gatgaactgc tgcagctcgc taggcaactg tacgagctgc tcgtccccca agctatcgga      360

gctaaaggag acgctcagca gatcgctagg aagtttctga gccccctcgc cgataaagac      420

gctgtgggcg gtttaggaat cgccaaggct ggaaataaac ctcgttgggt gaggatgagg      480

gaagctggcg agcccggctg ggaggaggaa aaggagaaag ccgaaactcg taaatccgcc      540

gacagaacag ctgacgtgct gagggccctc gccgacttcg gtttaaaacc cctcatgaga      600

gtctataccg actccgagat gtccagcgtc gagtggaaac ccttacgtaa gggccaagct      660

gtgagaacat gggatcgtga tatgttccag caagctatcg aaaggatgat gagctgggag      720

tcttggaatc agagagtggg ccaagaatac gccaaactcg tggaacagaa gaatcgtttc      780

gagcagaaaa actttgtcgg acaagaacat ttagtgcatc tcgtgaacca actgcagcaa      840

gatatgaagg aggcctcccc cggtttagag agcaaagagc aaaccgctca ctatgtcacc      900

ggtcgtgccc tcagaggctc cgacaaagtg ttcgaaaagt ggggaaagct ggcccccgac      960

gctcctttcg atttatacga cgccgagatc aagaacgtgc agaggagaaa tacaaggagg     1020

tttggctccc acgatttatt cgctaaactc gccgagcccg aataccaagc tttatggaga     1080

gaggatgcca gctttctgac tcgttacgcc gtgtacaatt ccattctgag aaaactgaac     1140

cacgccaaaa tgtttgccac attcacttta cccgatgcca ccgcccaccc tatctggaca     1200

agattcgaca agctcggcgg aaacctccac cagtatacct ttttatttaa cgaattcgga     1260

gagaggaggc atgccattcg ttttcacaag ttattaaagg tcgagaatgg agtcgccaga     1320

gaggtggacg acgtcaccgt gcctatcagc atgagcgaac agctggacaa tttactgcct     1380

cgtgacccca acgaacctat cgccctctac ttcagagact acggagctga gcagcatttc     1440

accggcgagt tcggaggcgc taagatccag tgtagaaggg atcaactggc ccatatgcat     1500

aggagaaggg gcgccagaga tgtctatctg aacgtgagcg ttcgtgtcca aagccaaagc     1560

gaggccagag gagaaaggag acccccctac gccgccgtct ttagactggt cggcgacaat     1620

catcgtgctt tcgtgcactt tgataagctg tccgactatt tagccgaaca ccccgacgat     1680

ggaaagctgg gcagcgaggg attattaagc ggcctcagag tcatgagcgt ggctctgggc     1740

ctcagaacca gcgcctccat ctccgtcttt agggtggcca gaaaagacga gctgaagccc     1800

aacagcaagg gaagggtgcc ctttttcttc cctatcaagg gcaatgacaa tttagtggcc     1860

gtgcacgaaa ggtcccagtt attaaagctg cccggcgaga ccgaaagcaa agatttaagg     1920

gctatcagag aggagagaca gagaacttta agacagctga gaacccagct ggcttatctg     1980

agattattag tcagatgtgg cagcgaggac gtcggtcgta gagagaggag ctgggccaag     2040

ctgattgaac aacccgttga tgccgctaat cacatgaccc ccgattggag ggaagctttc     2100

gagaacgagc tgcagaaact gaagtcttta cacggcattt gcagcgacaa ggagtggatg     2160

gacgccgtgt acgagtccgt gagaagggtg tggaggcaca tgggaaagca agttagggat     2220

tggaggaaag atgtgaggtc cggcgaaaga cccaagatca gaggctacgc caaggacgtg     2280

gtcggaggaa actccatcga gcagatcgag tacctcgaga gacaatacaa gtttttaaag     2340

tcttggtcct tcttcggcaa ggtcagcggc caagtcattc gtgctgaaaa gggatctcgt     2400

ttcgccatca cactgagaga gcacattgac cacgccaaag aggatcgtct gaaaaaactc     2460

gccgatcgta tcattatgga ggccctcggc tatgtctatg ctctggacga gagaggcaag     2520

ggaaaatggg tcgccaagta tcccccttgt caactgattt tattagcgga gctgtccgag     2580

taccaattta acaacgatag gcctccctcc gagaataacc agctcatgca gtggagccat     2640

cgtggcgtgt ttcaagaact gatcaatcaa gctcaagttc acgatttact cgtgggcacc     2700

atgtacgccg cttttagctc cagattcgac gctaggaccg gcgcccccgg tattaggtgt     2760

agaagagtgc ccgctcgttg cacccaagaa cataaccccg aaccctttcc ttggtggctg     2820

aataagttcg tcgtcgagca caccctcgac gcttgccctt tacgtgccga cgacctcatc     2880

cctactggtg aaggcgagat ctttgtgagc cctttcagcg ctgaggaagg cgattttcac     2940

cagatccacg ccgctctgaa cgccgcccag aatctgcaac agaggctctg gagcgacttc     3000

gatatttccc agattcgtct gaggtgcgat tggggagagg tggatggaga actcgtcctc     3060

atccccagac tgactggtaa gaggaccgct gacagctatt ccaataaggt cttctatacc     3120

aatactggtg tcacctacta cgagagagag aggggcaaga agagaaggaa agtcttcgcc     3180

caagagaagc tgtccgagga ggaggccgaa ttattagtcg aggctgatga ggctcgtgaa     3240

aagtccgtgg ttttaatgag ggacccctcc ggcatcatca acagaggcaa ttggactcgt     3300

cagaaggaat tctggagcat ggtgaatcag aggatcgagg gctatctggt gaagcagatt     3360

agatctcgtg tgcctctgca agatagcgct tgtgagaata ccggagatat tagcggcggg     3420

agcggcggga gcggggggag cactaatctg agcgacatca ttgagaagga gactgggaaa     3480

cagctggtca ttcaggagtc catcctgatg ctgcctgagg aggtggagga agtgatcggc     3540

aacaagccag agtctgacat cctggtgcac accgcctacg acgagtccac agatgagaat     3600

gtgatgctgc tgacctctga cgcccccgag tataagcctt gggccctggt catccaggat     3660

tctaacggcg agaataagat caagatgctg agcggaggat ccggaggatc tggaggcagc     3720

accaacctgt ctgacatcat cgagaaggag acaggcaagc agctggtcat ccaggagagc     3780

atcctaatgc ttcccgaaga agtcgaagaa gtgatcggaa acaagcctga gagcgatatc     3840

ctggtccata ctgcgtatga tgaaagtacc gacgaaaacg taatgctact cacatccgac     3900

gccccagagt ataagccctg ggctctagtt atacaagact ccaacggaga gaacaaaatc     3960

aaaatgctgt ctggcggctc aaaaagaacc gccgacggca gcgaattcga gcccaagaag     4020

aagaggaaag tctaa                                                      4035


<210>  73
<211>  1344
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein containing sequences from Cas12b and SV40 NLS

<400>  73

Met Ala Pro Lys Lys Lys Arg Lys Val Ala Val Lys Ser Ile Lys Val 
1               5                   10                  15      


Lys Leu Arg Leu Asp Asp Met Pro Glu Ile Arg Ala Gly Leu Trp Lys 
            20                  25                  30          


Leu His Lys Glu Val Asn Ala Gly Val Arg Tyr Tyr Thr Glu Trp Leu 
        35                  40                  45              


Ser Leu Leu Arg Gln Glu Asn Leu Tyr Arg Arg Ser Pro Asn Gly Asp 
    50                  55                  60                  


Gly Glu Gln Glu Cys Asp Lys Thr Ala Glu Glu Cys Lys Ala Glu Leu 
65                  70                  75                  80  


Leu Glu Arg Leu Arg Ala Arg Gln Val Glu Asn Gly His Arg Gly Pro 
                85                  90                  95      


Ala Gly Ser Asp Asp Glu Leu Leu Gln Leu Ala Arg Gln Leu Tyr Glu 
            100                 105                 110         


Leu Leu Val Pro Gln Ala Ile Gly Ala Lys Gly Asp Ala Gln Gln Ile 
        115                 120                 125             


Ala Arg Lys Phe Leu Ser Pro Leu Ala Asp Lys Asp Ala Val Gly Gly 
    130                 135                 140                 


Leu Gly Ile Ala Lys Ala Gly Asn Lys Pro Arg Trp Val Arg Met Arg 
145                 150                 155                 160 


Glu Ala Gly Glu Pro Gly Trp Glu Glu Glu Lys Glu Lys Ala Glu Thr 
                165                 170                 175     


Arg Lys Ser Ala Asp Arg Thr Ala Asp Val Leu Arg Ala Leu Ala Asp 
            180                 185                 190         


Phe Gly Leu Lys Pro Leu Met Arg Val Tyr Thr Asp Ser Glu Met Ser 
        195                 200                 205             


Ser Val Glu Trp Lys Pro Leu Arg Lys Gly Gln Ala Val Arg Thr Trp 
    210                 215                 220                 


Asp Arg Asp Met Phe Gln Gln Ala Ile Glu Arg Met Met Ser Trp Glu 
225                 230                 235                 240 


Ser Trp Asn Gln Arg Val Gly Gln Glu Tyr Ala Lys Leu Val Glu Gln 
                245                 250                 255     


Lys Asn Arg Phe Glu Gln Lys Asn Phe Val Gly Gln Glu His Leu Val 
            260                 265                 270         


His Leu Val Asn Gln Leu Gln Gln Asp Met Lys Glu Ala Ser Pro Gly 
        275                 280                 285             


Leu Glu Ser Lys Glu Gln Thr Ala His Tyr Val Thr Gly Arg Ala Leu 
    290                 295                 300                 


Arg Gly Ser Asp Lys Val Phe Glu Lys Trp Gly Lys Leu Ala Pro Asp 
305                 310                 315                 320 


Ala Pro Phe Asp Leu Tyr Asp Ala Glu Ile Lys Asn Val Gln Arg Arg 
                325                 330                 335     


Asn Thr Arg Arg Phe Gly Ser His Asp Leu Phe Ala Lys Leu Ala Glu 
            340                 345                 350         


Pro Glu Tyr Gln Ala Leu Trp Arg Glu Asp Ala Ser Phe Leu Thr Arg 
        355                 360                 365             


Tyr Ala Val Tyr Asn Ser Ile Leu Arg Lys Leu Asn His Ala Lys Met 
    370                 375                 380                 


Phe Ala Thr Phe Thr Leu Pro Asp Ala Thr Ala His Pro Ile Trp Thr 
385                 390                 395                 400 


Arg Phe Asp Lys Leu Gly Gly Asn Leu His Gln Tyr Thr Phe Leu Phe 
                405                 410                 415     


Asn Glu Phe Gly Glu Arg Arg His Ala Ile Arg Phe His Lys Leu Leu 
            420                 425                 430         


Lys Val Glu Asn Gly Val Ala Arg Glu Val Asp Asp Val Thr Val Pro 
        435                 440                 445             


Ile Ser Met Ser Glu Gln Leu Asp Asn Leu Leu Pro Arg Asp Pro Asn 
    450                 455                 460                 


Glu Pro Ile Ala Leu Tyr Phe Arg Asp Tyr Gly Ala Glu Gln His Phe 
465                 470                 475                 480 


Thr Gly Glu Phe Gly Gly Ala Lys Ile Gln Cys Arg Arg Asp Gln Leu 
                485                 490                 495     


Ala His Met His Arg Arg Arg Gly Ala Arg Asp Val Tyr Leu Asn Val 
            500                 505                 510         


Ser Val Arg Val Gln Ser Gln Ser Glu Ala Arg Gly Glu Arg Arg Pro 
        515                 520                 525             


Pro Tyr Ala Ala Val Phe Arg Leu Val Gly Asp Asn His Arg Ala Phe 
    530                 535                 540                 


Val His Phe Asp Lys Leu Ser Asp Tyr Leu Ala Glu His Pro Asp Asp 
545                 550                 555                 560 


Gly Lys Leu Gly Ser Glu Gly Leu Leu Ser Gly Leu Arg Val Met Ser 
                565                 570                 575     


Val Ala Leu Gly Leu Arg Thr Ser Ala Ser Ile Ser Val Phe Arg Val 
            580                 585                 590         


Ala Arg Lys Asp Glu Leu Lys Pro Asn Ser Lys Gly Arg Val Pro Phe 
        595                 600                 605             


Phe Phe Pro Ile Lys Gly Asn Asp Asn Leu Val Ala Val His Glu Arg 
    610                 615                 620                 


Ser Gln Leu Leu Lys Leu Pro Gly Glu Thr Glu Ser Lys Asp Leu Arg 
625                 630                 635                 640 


Ala Ile Arg Glu Glu Arg Gln Arg Thr Leu Arg Gln Leu Arg Thr Gln 
                645                 650                 655     


Leu Ala Tyr Leu Arg Leu Leu Val Arg Cys Gly Ser Glu Asp Val Gly 
            660                 665                 670         


Arg Arg Glu Arg Ser Trp Ala Lys Leu Ile Glu Gln Pro Val Asp Ala 
        675                 680                 685             


Ala Asn His Met Thr Pro Asp Trp Arg Glu Ala Phe Glu Asn Glu Leu 
    690                 695                 700                 


Gln Lys Leu Lys Ser Leu His Gly Ile Cys Ser Asp Lys Glu Trp Met 
705                 710                 715                 720 


Asp Ala Val Tyr Glu Ser Val Arg Arg Val Trp Arg His Met Gly Lys 
                725                 730                 735     


Gln Val Arg Asp Trp Arg Lys Asp Val Arg Ser Gly Glu Arg Pro Lys 
            740                 745                 750         


Ile Arg Gly Tyr Ala Lys Asp Val Val Gly Gly Asn Ser Ile Glu Gln 
        755                 760                 765             


Ile Glu Tyr Leu Glu Arg Gln Tyr Lys Phe Leu Lys Ser Trp Ser Phe 
    770                 775                 780                 


Phe Gly Lys Val Ser Gly Gln Val Ile Arg Ala Glu Lys Gly Ser Arg 
785                 790                 795                 800 


Phe Ala Ile Thr Leu Arg Glu His Ile Asp His Ala Lys Glu Asp Arg 
                805                 810                 815     


Leu Lys Lys Leu Ala Asp Arg Ile Ile Met Glu Ala Leu Gly Tyr Val 
            820                 825                 830         


Tyr Ala Leu Asp Glu Arg Gly Lys Gly Lys Trp Val Ala Lys Tyr Pro 
        835                 840                 845             


Pro Cys Gln Leu Ile Leu Leu Ala Glu Leu Ser Glu Tyr Gln Phe Asn 
    850                 855                 860                 


Asn Asp Arg Pro Pro Ser Glu Asn Asn Gln Leu Met Gln Trp Ser His 
865                 870                 875                 880 


Arg Gly Val Phe Gln Glu Leu Ile Asn Gln Ala Gln Val His Asp Leu 
                885                 890                 895     


Leu Val Gly Thr Met Tyr Ala Ala Phe Ser Ser Arg Phe Asp Ala Arg 
            900                 905                 910         


Thr Gly Ala Pro Gly Ile Arg Cys Arg Arg Val Pro Ala Arg Cys Thr 
        915                 920                 925             


Gln Glu His Asn Pro Glu Pro Phe Pro Trp Trp Leu Asn Lys Phe Val 
    930                 935                 940                 


Val Glu His Thr Leu Asp Ala Cys Pro Leu Arg Ala Asp Asp Leu Ile 
945                 950                 955                 960 


Pro Thr Gly Glu Gly Glu Ile Phe Val Ser Pro Phe Ser Ala Glu Glu 
                965                 970                 975     


Gly Asp Phe His Gln Ile His Ala Ala Leu Asn Ala Ala Gln Asn Leu 
            980                 985                 990         


Gln Gln Arg Leu Trp Ser Asp Phe  Asp Ile Ser Gln Ile  Arg Leu Arg 
        995                 1000                 1005             


Cys Asp  Trp Gly Glu Val Asp  Gly Glu Leu Val Leu  Ile Pro Arg 
    1010                 1015                 1020             


Leu Thr  Gly Lys Arg Thr Ala  Asp Ser Tyr Ser Asn  Lys Val Phe 
    1025                 1030                 1035             


Tyr Thr  Asn Thr Gly Val Thr  Tyr Tyr Glu Arg Glu  Arg Gly Lys 
    1040                 1045                 1050             


Lys Arg  Arg Lys Val Phe Ala  Gln Glu Lys Leu Ser  Glu Glu Glu 
    1055                 1060                 1065             


Ala Glu  Leu Leu Val Glu Ala  Asp Glu Ala Arg Glu  Lys Ser Val 
    1070                 1075                 1080             


Val Leu  Met Arg Asp Pro Ser  Gly Ile Ile Asn Arg  Gly Asn Trp 
    1085                 1090                 1095             


Thr Arg  Gln Lys Glu Phe Trp  Ser Met Val Asn Gln  Arg Ile Glu 
    1100                 1105                 1110             


Gly Tyr  Leu Val Lys Gln Ile  Arg Ser Arg Val Pro  Leu Gln Asp 
    1115                 1120                 1125             


Ser Ala  Cys Glu Asn Thr Gly  Asp Ile Ser Gly Gly  Ser Gly Gly 
    1130                 1135                 1140             


Ser Gly  Gly Ser Thr Asn Leu  Ser Asp Ile Ile Glu  Lys Glu Thr 
    1145                 1150                 1155             


Gly Lys  Gln Leu Val Ile Gln  Glu Ser Ile Leu Met  Leu Pro Glu 
    1160                 1165                 1170             


Glu Val  Glu Glu Val Ile Gly  Asn Lys Pro Glu Ser  Asp Ile Leu 
    1175                 1180                 1185             


Val His  Thr Ala Tyr Asp Glu  Ser Thr Asp Glu Asn  Val Met Leu 
    1190                 1195                 1200             


Leu Thr  Ser Asp Ala Pro Glu  Tyr Lys Pro Trp Ala  Leu Val Ile 
    1205                 1210                 1215             


Gln Asp  Ser Asn Gly Glu Asn  Lys Ile Lys Met Leu  Ser Gly Gly 
    1220                 1225                 1230             


Ser Gly  Gly Ser Gly Gly Ser  Thr Asn Leu Ser Asp  Ile Ile Glu 
    1235                 1240                 1245             


Lys Glu  Thr Gly Lys Gln Leu  Val Ile Gln Glu Ser  Ile Leu Met 
    1250                 1255                 1260             


Leu Pro  Glu Glu Val Glu Glu  Val Ile Gly Asn Lys  Pro Glu Ser 
    1265                 1270                 1275             


Asp Ile  Leu Val His Thr Ala  Tyr Asp Glu Ser Thr  Asp Glu Asn 
    1280                 1285                 1290             


Val Met  Leu Leu Thr Ser Asp  Ala Pro Glu Tyr Lys  Pro Trp Ala 
    1295                 1300                 1305             


Leu Val  Ile Gln Asp Ser Asn  Gly Glu Asn Lys Ile  Lys Met Leu 
    1310                 1315                 1320             


Ser Gly  Gly Ser Lys Arg Thr  Ala Asp Gly Ser Glu  Phe Glu Pro 
    1325                 1330                 1335             


Lys Lys  Lys Arg Lys Val 
    1340                 


<210>  74
<211>  2235
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid containing sequences from humanized CasMINI and SV40 NLS

<400>  74
atggccccca agaaaaaacg caaggtggcc aaaaacacca ttaccaaaac actgaaactg       60

cgtattgtgc gtccgtataa tagcgcagaa gtggaaaaaa ttgttgccga cgaaaaaaac      120

aaccgcgaaa aaatcgcact ggaaaagaac aaagacaaag tgaaagaagc ctgcagcaaa      180

catctgaaag ttgcagcata ttgtaccaca caggttgaac gtaatgcatg cctgttttgt      240

aaagcacgta aactggatga caaattctac caaaaactgc gtggtcagtt tccggatgca      300

gttttttggc aagaaatcag cgaaattttt cgccagctgc agaaacaggc agcagaaatc      360

tataatcaga gcctgatcga actgtactac gagattttta tcaaaggcaa aggtattgca      420

aatgccagca gcgttgaaca ttatctgagt agagtttgtt atagacgtgc agcagaactg      480

tttaaaaacg cagcaattgc aagcggtctg cgtagcaaaa tcaaaagcaa ttttcgtctg      540

aaagaactga aaaacatgaa aagtggtctg ccgaccacca aaagcgataa ttttccgatt      600

ccgctggtta aacagaaagg tggtcagtat accggttttg aaattagcaa tcataatagc      660

gacttcatca tcaagattcc gtttggtcgt tggcaggtca aaaaagagat tgataaatat      720

cgtccgtggg agaaatttga ctttgaacag gttcagaaaa gcccgaaacc gattagcctg      780

ctgctgagca cccagcgtcg taaacgtaat aaaggttgga gcaaagatga aggcaccgaa      840

gccgaaatca aaaaagttat gaatggcgat tatcagacca gctacattga agttaaacgt      900

ggcagcaaaa tctgtgaaaa aagcgcatgg atgctgaatc tgagcattga tgttccgaaa      960

attgataaag gtgtggatcc gagcattatt ggtggtattg cagttggtgt tagatcaccg     1020

ctggtttgcg caattaacaa tgcatttagc cgttatagca tcagcgataa cgacctgttt     1080

cacttcaaca agaaaatgtt tgcacgtcgt cgtatcctgc tgaaaaaaaa ccgtcataaa     1140

cgtgcaggtc atggtgcaaa aaacaaactg aaaccgatca ccattctgac cgaaaaaagt     1200

gaacgttttc gcaaaaagct gattgaacgt tgggcatgtg aaatcgcgga tttcttcatt     1260

aaaaacaaag ttggcaccgt gcagatggaa aatctggaaa gcatgaaacg taaagaggac     1320

agctatttta acattcgcct gcgtggcttt tggccgtatg cagaaatgca gaacaaaatc     1380

gaattcaaac tgaagcagta tggcatcgaa attcgtaaag ttgcaccgaa taataccagc     1440

aaaacctgta gcaaatgtgg ccatctgaac aactatttca acttcgagta ccgcaagaaa     1500

aacaaattcc cgcactttaa atgcgaaaaa tgcaacttca aagaaaacgc cgcgtataat     1560

gcagccctga atatttcaaa cccgaaactg aaaagcacca aagagagacc gagcggcggg     1620

agcggcggga gcggggggag cactaatctg agcgacatca ttgagaagga gactgggaaa     1680

cagctggtca ttcaggagtc catcctgatg ctgcctgagg aggtggagga agtgatcggc     1740

aacaagccag agtctgacat cctggtgcac accgcctacg acgagtccac agatgagaat     1800

gtgatgctgc tgacctctga cgcccccgag tataagcctt gggccctggt catccaggat     1860

tctaacggcg agaataagat caagatgctg agcggaggat ccggaggatc tggaggcagc     1920

accaacctgt ctgacatcat cgagaaggag acaggcaagc agctggtcat ccaggagagc     1980

atcctgatgc tgcccgaaga agtcgaagaa gtgatcggaa acaagcctga gagcgatatc     2040

ctggtccata ccgcctacga cgagagtacc gacgaaaatg tgatgctgct gacatccgac     2100

gccccagagt ataagccctg ggctctggtc atccaggatt ccaacggaga gaacaaaatc     2160

aaaatgctgt ctggcggctc aaaaagaacc gccgacggca gcgaattcga gcccaagaag     2220

aagaggaaag tctaa                                                      2235


<210>  75
<211>  744
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein containing sequences from CasMINI and SV40 NLS

<400>  75

Met Ala Pro Lys Lys Lys Arg Lys Val Ala Lys Asn Thr Ile Thr Lys 
1               5                   10                  15      


Thr Leu Lys Leu Arg Ile Val Arg Pro Tyr Asn Ser Ala Glu Val Glu 
            20                  25                  30          


Lys Ile Val Ala Asp Glu Lys Asn Asn Arg Glu Lys Ile Ala Leu Glu 
        35                  40                  45              


Lys Asn Lys Asp Lys Val Lys Glu Ala Cys Ser Lys His Leu Lys Val 
    50                  55                  60                  


Ala Ala Tyr Cys Thr Thr Gln Val Glu Arg Asn Ala Cys Leu Phe Cys 
65                  70                  75                  80  


Lys Ala Arg Lys Leu Asp Asp Lys Phe Tyr Gln Lys Leu Arg Gly Gln 
                85                  90                  95      


Phe Pro Asp Ala Val Phe Trp Gln Glu Ile Ser Glu Ile Phe Arg Gln 
            100                 105                 110         


Leu Gln Lys Gln Ala Ala Glu Ile Tyr Asn Gln Ser Leu Ile Glu Leu 
        115                 120                 125             


Tyr Tyr Glu Ile Phe Ile Lys Gly Lys Gly Ile Ala Asn Ala Ser Ser 
    130                 135                 140                 


Val Glu His Tyr Leu Ser Arg Val Cys Tyr Arg Arg Ala Ala Glu Leu 
145                 150                 155                 160 


Phe Lys Asn Ala Ala Ile Ala Ser Gly Leu Arg Ser Lys Ile Lys Ser 
                165                 170                 175     


Asn Phe Arg Leu Lys Glu Leu Lys Asn Met Lys Ser Gly Leu Pro Thr 
            180                 185                 190         


Thr Lys Ser Asp Asn Phe Pro Ile Pro Leu Val Lys Gln Lys Gly Gly 
        195                 200                 205             


Gln Tyr Thr Gly Phe Glu Ile Ser Asn His Asn Ser Asp Phe Ile Ile 
    210                 215                 220                 


Lys Ile Pro Phe Gly Arg Trp Gln Val Lys Lys Glu Ile Asp Lys Tyr 
225                 230                 235                 240 


Arg Pro Trp Glu Lys Phe Asp Phe Glu Gln Val Gln Lys Ser Pro Lys 
                245                 250                 255     


Pro Ile Ser Leu Leu Leu Ser Thr Gln Arg Arg Lys Arg Asn Lys Gly 
            260                 265                 270         


Trp Ser Lys Asp Glu Gly Thr Glu Ala Glu Ile Lys Lys Val Met Asn 
        275                 280                 285             


Gly Asp Tyr Gln Thr Ser Tyr Ile Glu Val Lys Arg Gly Ser Lys Ile 
    290                 295                 300                 


Cys Glu Lys Ser Ala Trp Met Leu Asn Leu Ser Ile Asp Val Pro Lys 
305                 310                 315                 320 


Ile Asp Lys Gly Val Asp Pro Ser Ile Ile Gly Gly Ile Ala Val Gly 
                325                 330                 335     


Val Arg Ser Pro Leu Val Cys Ala Ile Asn Asn Ala Phe Ser Arg Tyr 
            340                 345                 350         


Ser Ile Ser Asp Asn Asp Leu Phe His Phe Asn Lys Lys Met Phe Ala 
        355                 360                 365             


Arg Arg Arg Ile Leu Leu Lys Lys Asn Arg His Lys Arg Ala Gly His 
    370                 375                 380                 


Gly Ala Lys Asn Lys Leu Lys Pro Ile Thr Ile Leu Thr Glu Lys Ser 
385                 390                 395                 400 


Glu Arg Phe Arg Lys Lys Leu Ile Glu Arg Trp Ala Cys Glu Ile Ala 
                405                 410                 415     


Asp Phe Phe Ile Lys Asn Lys Val Gly Thr Val Gln Met Glu Asn Leu 
            420                 425                 430         


Glu Ser Met Lys Arg Lys Glu Asp Ser Tyr Phe Asn Ile Arg Leu Arg 
        435                 440                 445             


Gly Phe Trp Pro Tyr Ala Glu Met Gln Asn Lys Ile Glu Phe Lys Leu 
    450                 455                 460                 


Lys Gln Tyr Gly Ile Glu Ile Arg Lys Val Ala Pro Asn Asn Thr Ser 
465                 470                 475                 480 


Lys Thr Cys Ser Lys Cys Gly His Leu Asn Asn Tyr Phe Asn Phe Glu 
                485                 490                 495     


Tyr Arg Lys Lys Asn Lys Phe Pro His Phe Lys Cys Glu Lys Cys Asn 
            500                 505                 510         


Phe Lys Glu Asn Ala Ala Tyr Asn Ala Ala Leu Asn Ile Ser Asn Pro 
        515                 520                 525             


Lys Leu Lys Ser Thr Lys Glu Arg Pro Ser Gly Gly Ser Gly Gly Ser 
    530                 535                 540                 


Gly Gly Ser Thr Asn Leu Ser Asp Ile Ile Glu Lys Glu Thr Gly Lys 
545                 550                 555                 560 


Gln Leu Val Ile Gln Glu Ser Ile Leu Met Leu Pro Glu Glu Val Glu 
                565                 570                 575     


Glu Val Ile Gly Asn Lys Pro Glu Ser Asp Ile Leu Val His Thr Ala 
            580                 585                 590         


Tyr Asp Glu Ser Thr Asp Glu Asn Val Met Leu Leu Thr Ser Asp Ala 
        595                 600                 605             


Pro Glu Tyr Lys Pro Trp Ala Leu Val Ile Gln Asp Ser Asn Gly Glu 
    610                 615                 620                 


Asn Lys Ile Lys Met Leu Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser 
625                 630                 635                 640 


Thr Asn Leu Ser Asp Ile Ile Glu Lys Glu Thr Gly Lys Gln Leu Val 
                645                 650                 655     


Ile Gln Glu Ser Ile Leu Met Leu Pro Glu Glu Val Glu Glu Val Ile 
            660                 665                 670         


Gly Asn Lys Pro Glu Ser Asp Ile Leu Val His Thr Ala Tyr Asp Glu 
        675                 680                 685             


Ser Thr Asp Glu Asn Val Met Leu Leu Thr Ser Asp Ala Pro Glu Tyr 
    690                 695                 700                 


Lys Pro Trp Ala Leu Val Ile Gln Asp Ser Asn Gly Glu Asn Lys Ile 
705                 710                 715                 720 


Lys Met Leu Ser Gly Gly Ser Lys Arg Thr Ala Asp Gly Ser Glu Phe 
                725                 730                 735     


Glu Pro Lys Lys Lys Arg Lys Val 
            740                 


<210>  76
<211>  1050
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid containing sequences from human Apobec3A and SV40 NLS

<400>  76
atggccccca agaagaagcg gaaagtggaa gccagcccag catccgggcc cagacacttg       60

atggatccac acatattcac ttccaacttt aacaatggca ttggaaggca taagacctac      120

ctgtgctacg aagtggagcg cctggacaat ggcacctcgg tcaagatgga ccagcacagg      180

ggctttctac acaaccaggc taagaatctt ctctgtggct tttacggccg ccatgcggag      240

ctgcgcttct tggacctggt tccttctttg cagttggacc cggcccaaat ctacagggtc      300

acttggttca tctcctggag cccctgcttc tccgcgggct gtgccgggga agtgcgtgcg      360

ttccttcagg agaacacaca cgtgagactg cgtatcttcg ctgcccgcat ctatgatgac      420

gaccccctat ataaggaggc actgcaaatg ctgcgggatg ctggggccca agtctccatc      480

atgacctacg atgaatttaa gcactgctgg gacacctttg tggaccacca gggatgtccc      540

ttccagccct gggatggact agatgagcac agccaagccc tgagtgggag gctgcgggcc      600

attctccaga atcagggaaa cgagctgaag acacccctgg gcgacaccac acacacctct      660

ccaccttgcc cagcaccaga gctgctggga ggccctatgg ccagcaactt cacacagttt      720

gtgctggtgg ataatggagg aaccggcgac gtgacagtgg caccatctaa ctttgccaat      780

ggcatcgccg agtggatcag ctccaactct cggagccagg cctataaggt gacctgtagc      840

gtgcggcagt ctagcgccca gaatagaaag tatacaatca aggtggaggt gcctaagggc      900

gcctggagat cctacctgaa catggagctg accatcccaa tctttgccac aaattctgat      960

tgcgagctga tcgtgaaggc catgcagggc ctgctgaagg acggcaaccc tatcccaagc     1020

gccatcgccg ccaatagcgg aatctactga                                      1050


<210>  77
<211>  349
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein containing sequences from human Apobec3A and SV40 NLS

<400>  77

Met Ala Pro Lys Lys Lys Arg Lys Val Glu Ala Ser Pro Ala Ser Gly 
1               5                   10                  15      


Pro Arg His Leu Met Asp Pro His Ile Phe Thr Ser Asn Phe Asn Asn 
            20                  25                  30          


Gly Ile Gly Arg His Lys Thr Tyr Leu Cys Tyr Glu Val Glu Arg Leu 
        35                  40                  45              


Asp Asn Gly Thr Ser Val Lys Met Asp Gln His Arg Gly Phe Leu His 
    50                  55                  60                  


Asn Gln Ala Lys Asn Leu Leu Cys Gly Phe Tyr Gly Arg His Ala Glu 
65                  70                  75                  80  


Leu Arg Phe Leu Asp Leu Val Pro Ser Leu Gln Leu Asp Pro Ala Gln 
                85                  90                  95      


Ile Tyr Arg Val Thr Trp Phe Ile Ser Trp Ser Pro Cys Phe Ser Ala 
            100                 105                 110         


Gly Cys Ala Gly Glu Val Arg Ala Phe Leu Gln Glu Asn Thr His Val 
        115                 120                 125             


Arg Leu Arg Ile Phe Ala Ala Arg Ile Tyr Asp Asp Asp Pro Leu Tyr 
    130                 135                 140                 


Lys Glu Ala Leu Gln Met Leu Arg Asp Ala Gly Ala Gln Val Ser Ile 
145                 150                 155                 160 


Met Thr Tyr Asp Glu Phe Lys His Cys Trp Asp Thr Phe Val Asp His 
                165                 170                 175     


Gln Gly Cys Pro Phe Gln Pro Trp Asp Gly Leu Asp Glu His Ser Gln 
            180                 185                 190         


Ala Leu Ser Gly Arg Leu Arg Ala Ile Leu Gln Asn Gln Gly Asn Glu 
        195                 200                 205             


Leu Lys Thr Pro Leu Gly Asp Thr Thr His Thr Ser Pro Pro Cys Pro 
    210                 215                 220                 


Ala Pro Glu Leu Leu Gly Gly Pro Met Ala Ser Asn Phe Thr Gln Phe 
225                 230                 235                 240 


Val Leu Val Asp Asn Gly Gly Thr Gly Asp Val Thr Val Ala Pro Ser 
                245                 250                 255     


Asn Phe Ala Asn Gly Ile Ala Glu Trp Ile Ser Ser Asn Ser Arg Ser 
            260                 265                 270         


Gln Ala Tyr Lys Val Thr Cys Ser Val Arg Gln Ser Ser Ala Gln Asn 
        275                 280                 285             


Arg Lys Tyr Thr Ile Lys Val Glu Val Pro Lys Gly Ala Trp Arg Ser 
    290                 295                 300                 


Tyr Leu Asn Met Glu Leu Thr Ile Pro Ile Phe Ala Thr Asn Ser Asp 
305                 310                 315                 320 


Cys Glu Leu Ile Val Lys Ala Met Gln Gly Leu Leu Lys Asp Gly Asn 
                325                 330                 335     


Pro Ile Pro Ser Ala Ile Ala Ala Asn Ser Gly Ile Tyr 
            340                 345                 


<210>  78
<211>  1008
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid containing sequences from Anolis Apobec1a and SV40 NLS

<400>  78
atggccccca agaagaagcg gaaagtgggg tatcaggctg caattctatt atcaaatttg       60

ttcttcaggt ggcaaatgga accagaggcg tttcagagga attttgatcc cagagaattt      120

cccgagtgta ctttactgct gtatgaaatc cactgggata acaacaccag taggaactgg      180

tgtacaaaca aacctggcct ccatgctgaa gaaaattttt tgcaaatttt taatgagaaa      240

atagatatca ggcaggacac accatgctct atcacttggt ttctgtcttg gagtccttgt      300

tatccatgca gccaggctat aattaagttc ttggaagcac accctaacgt gagcctggag      360

ataaaagctg ctcggctgta catgcatcaa atcgactgta acaaggaagg cctcaggaat      420

ttaggtagaa atagagtttc tatcatgaat ctacctgatt atcgccactg ttggacaaca      480

tttgtggttc ctagaggggc aaatgaagat tattggccac aggatttctt accagccata      540

acaaattatt ccagggaact tgactcaatt cttcaggacg agctgaagac acccctgggc      600

gacaccacac acacctctcc accttgccca gcaccagagc tgctgggagg ccctatggcc      660

agcaacttca cacagtttgt gctggtggat aatggaggaa ccggcgacgt gacagtggca      720

ccatctaact ttgccaatgg catcgccgag tggatcagct ccaactctcg gagccaggcc      780

tataaggtga cctgtagcgt gcggcagtct agcgcccaga atagaaagta tacaatcaag      840

gtggaggtgc ctaagggcgc ctggagatcc tacctgaaca tggagctgac catcccaatc      900

tttgccacaa attctgattg cgagctgatc gtgaaggcca tgcagggcct gctgaaggac      960

ggcaacccta tcccaagcgc catcgccgcc aatagcggaa tctactga                  1008


<210>  79
<211>  335
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein containing sequences from Anolis Apobec1a and SV40 NLS

<400>  79

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Tyr Gln Ala Ala Ile Leu 
1               5                   10                  15      


Leu Ser Asn Leu Phe Phe Arg Trp Gln Met Glu Pro Glu Ala Phe Gln 
            20                  25                  30          


Arg Asn Phe Asp Pro Arg Glu Phe Pro Glu Cys Thr Leu Leu Leu Tyr 
        35                  40                  45              


Glu Ile His Trp Asp Asn Asn Thr Ser Arg Asn Trp Cys Thr Asn Lys 
    50                  55                  60                  


Pro Gly Leu His Ala Glu Glu Asn Phe Leu Gln Ile Phe Asn Glu Lys 
65                  70                  75                  80  


Ile Asp Ile Arg Gln Asp Thr Pro Cys Ser Ile Thr Trp Phe Leu Ser 
                85                  90                  95      


Trp Ser Pro Cys Tyr Pro Cys Ser Gln Ala Ile Ile Lys Phe Leu Glu 
            100                 105                 110         


Ala His Pro Asn Val Ser Leu Glu Ile Lys Ala Ala Arg Leu Tyr Met 
        115                 120                 125             


His Gln Ile Asp Cys Asn Lys Glu Gly Leu Arg Asn Leu Gly Arg Asn 
    130                 135                 140                 


Arg Val Ser Ile Met Asn Leu Pro Asp Tyr Arg His Cys Trp Thr Thr 
145                 150                 155                 160 


Phe Val Val Pro Arg Gly Ala Asn Glu Asp Tyr Trp Pro Gln Asp Phe 
                165                 170                 175     


Leu Pro Ala Ile Thr Asn Tyr Ser Arg Glu Leu Asp Ser Ile Leu Gln 
            180                 185                 190         


Asp Glu Leu Lys Thr Pro Leu Gly Asp Thr Thr His Thr Ser Pro Pro 
        195                 200                 205             


Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Met Ala Ser Asn Phe Thr 
    210                 215                 220                 


Gln Phe Val Leu Val Asp Asn Gly Gly Thr Gly Asp Val Thr Val Ala 
225                 230                 235                 240 


Pro Ser Asn Phe Ala Asn Gly Ile Ala Glu Trp Ile Ser Ser Asn Ser 
                245                 250                 255     


Arg Ser Gln Ala Tyr Lys Val Thr Cys Ser Val Arg Gln Ser Ser Ala 
            260                 265                 270         


Gln Asn Arg Lys Tyr Thr Ile Lys Val Glu Val Pro Lys Gly Ala Trp 
        275                 280                 285             


Arg Ser Tyr Leu Asn Met Glu Leu Thr Ile Pro Ile Phe Ala Thr Asn 
    290                 295                 300                 


Ser Asp Cys Glu Leu Ile Val Lys Ala Met Gln Gly Leu Leu Lys Asp 
305                 310                 315                 320 


Gly Asn Pro Ile Pro Ser Ala Ile Ala Ala Asn Ser Gly Ile Tyr 
                325                 330                 335 


<210>  80
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  80
tgttccagtt tcctttacag                                                   20


<210>  81
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  81
cagcccgctg gccctgtaaa                                                   20


<210>  82
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  82
gccagagccg gggtgtgcag                                                   20


<210>  83
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  83
ggaagtgtcc agggatgctt                                                   20


<210>  84
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  84
tcgcgccctc ccagccgggc                                                   20


<210>  85
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  85
ctcctggacc ccctatttct gac                                               23


<210>  86
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  86
gccagagccg gggtgtgcag acg                                               23


<210>  87
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  87
aggacgtctg cccaatatgt                                                   20


<210>  88
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  88
ccaagtgaga agccagtgga                                                   20


<210>  89
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  89
tcttccctcc cagtcactga                                                   20


<210>  90
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  90
tcactctcga agacgctgct                                                   20


<210>  91
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  91
cgatgaggag acactccaag ag                                                22


<210>  92
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  92
gcctttctga aggtcatagt gc                                                22


<210>  93
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  93
aggctggccc gccccgcagt                                                   20


<210>  94
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  94
ccgatattcc tcaggtactc                                                   20


<210>  95
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  95
aggtttactc acgtcatcca                                                   20


<210>  96
<211>  19
<212>  DNA
<213>  Unknown

<220>
<223>  Qbeta phage operator stem loop

<400>  96
atgctgtcta agacagcat                                                    19


<210>  97
<211>  133
<212>  PRT
<213>  Unknown

<220>
<223>  Qbeta coat protein

<400>  97

Met Ala Lys Leu Glu Thr Val Thr Leu Gly Asn Ile Gly Lys Asp Gly 
1               5                   10                  15      


Lys Gln Thr Leu Val Leu Asn Pro Arg Gly Val Asn Pro Thr Asn Gly 
            20                  25                  30          


Val Ala Ser Leu Ser Gln Ala Gly Ala Val Pro Ala Leu Glu Lys Arg 
        35                  40                  45              


Val Thr Val Ser Val Ser Gln Pro Ser Arg Asn Arg Lys Asn Tyr Lys 
    50                  55                  60                  


Val His Val Lys Ile Gln Asn Pro Thr Ala Cys Thr Ala Asn Gly Ser 
65                  70                  75                  80  


Cys Asp Pro Ser Val Thr Arg Gln Ala Tyr Ala Asp Val Thr Phe Ser 
                85                  90                  95      


Phe Thr Gln Tyr Ser Thr Asp Glu Glu Arg Ala Phe Val Arg Thr Glu 
            100                 105                 110         


Leu Ala Ala Leu Leu Ala Ser Pro Leu Leu Ile Asp Ala Ile Asp Gln 
        115                 120                 125             


Leu Asn Pro Ala Tyr 
    130             


