                         SEQUENCE LISTING

<110>  INSCRIPTA, INC.
 
<120>  MAD NUCLEASES

<130>  INSC084PCT

<140>  PCT/US22/11052
<141>  2022-01-03

<150>  63/134,938
<151>  2021-01-07

<160>  73    

<170>  PatentIn version 3.5

<210>  1
<211>  1400
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ENGINEERED NUCLEASE

<400>  1

Met Ala Ala Phe Gln Arg Ser Tyr Thr Met Asn Leu Lys Pro Ala Thr 
1               5                   10                  15      


Ser Glu Gln Asp Lys Phe Ile Leu Trp Asn Arg Leu Phe Leu Thr His 
            20                  25                  30          


Trp Ser Val Asn Glu Gly Ala Lys Ile Phe Gly Glu Leu Phe Leu Asn 
        35                  40                  45              


Leu Arg Gly Gly Leu Ser Pro Glu Leu Asp Ile Phe Asp Leu Asp Lys 
    50                  55                  60                  


Val Lys Asp Asp Lys Lys Lys Lys Ala Phe Val Met Gly Arg Arg Arg 
65                  70                  75                  80  


Leu Leu Ala Leu Gly Trp Leu Ser Val Glu Asp Asn Leu Ser Ala Gly 
                85                  90                  95      


Glu His Pro Phe Arg Ile Arg Glu Ile Pro Val Gly Arg Asn Met Gly 
            100                 105                 110         


Lys Ser Gln Ala Ser Thr Leu Leu Thr Glu Ile Leu Lys Asn Lys Gly 
        115                 120                 125             


Ile Lys Asp Glu Ala Val Ile Lys Glu Trp Ile Asp Asp Cys Thr Pro 
    130                 135                 140                 


Ser Leu Ile Ala Asn Ile Arg Glu Asp Ala Val Trp Ile Asn Arg Ala 
145                 150                 155                 160 


Ser Ser Phe Asn Ser Ile Thr Pro Cys Pro Thr Lys Asp Glu Val Trp 
                165                 170                 175     


Ile Val Leu Ser Gly Leu Leu Gly Leu Arg Phe Leu Asp Leu Ser Leu 
            180                 185                 190         


Glu Glu Val Lys Gly Lys Glu Thr Glu Tyr Leu Tyr Asp Leu Gly Glu 
        195                 200                 205             


Lys Glu Thr Gln Ser Lys Ser Asp Pro Ser Lys Lys Ala Arg Glu Leu 
    210                 215                 220                 


Phe Gly Asn Leu Phe Thr Gln Asn Pro Val Leu Met Lys Asn Ser Arg 
225                 230                 235                 240 


Asp Lys Lys Asp Thr Phe Ala Lys Glu Phe Tyr Leu Ala Phe Lys Glu 
                245                 250                 255     


Phe Lys Asp Tyr Glu Lys Leu Lys Glu Lys Ile Glu Ser Trp Arg Lys 
            260                 265                 270         


Glu Lys Glu Phe Pro Leu Ile Glu Asn Pro Val Ala Glu Lys Tyr Pro 
        275                 280                 285             


Pro Glu Val Thr Phe Thr Gly Ser Pro Cys Thr Val Ser Lys Arg Tyr 
    290                 295                 300                 


Arg Lys Leu Leu Val Ser Leu Glu Leu Trp Pro Ser Ser Gln Asp Glu 
305                 310                 315                 320 


Asn Gly Asn Ile Pro Lys Thr Glu Lys Thr Glu Asp Lys Thr His Asn 
                325                 330                 335     


Gln Val Leu Leu Asp Tyr Leu Leu Lys Ala Cys Asn Glu Gly Asn Lys 
            340                 345                 350         


Gly Thr Gln Lys Ile Ile Thr Pro Val Trp Ala Asn Asn Leu Lys Ala 
        355                 360                 365             


Glu Leu Glu Leu Lys Met Asn Glu Ile Ile Arg Ile Gly Glu Ser Ser 
    370                 375                 380                 


Ser Thr Glu Leu Gln Arg Leu Met Ile Lys Met Ala Ala Arg Arg Ile 
385                 390                 395                 400 


Ser Gln Thr Leu Ser Trp Ile Lys Ile Asn Glu Gln Thr Lys His Asp 
                405                 410                 415     


Ala Tyr Gln Lys Lys Asn Lys Ala Phe Lys Leu Leu Ser Glu Ile Asp 
            420                 425                 430         


Lys Asn Gly Glu Ala Cys Lys Trp Leu Glu Asn Tyr Glu Leu Phe Arg 
        435                 440                 445             


Thr Asp Asp Ser Gly Gly Glu Glu Tyr His Ile Ser Leu Arg Ala Ile 
    450                 455                 460                 


Ser Cys Trp Lys Gln Ile Leu Glu Glu Trp Gln Lys Asn Asp Ser Pro 
465                 470                 475                 480 


Lys Ala Leu Arg Glu Lys Val Lys Glu Val Gln Ala Glu Glu Glu Lys 
                485                 490                 495     


Phe Gly Asp Ala Arg Leu Phe Glu Asp Leu Ala Asp Asp Asn Ala Arg 
            500                 505                 510         


Ser Val Trp Leu Leu Pro Asp Gly Asn Lys Thr Pro Asp Ile Leu Asn 
        515                 520                 525             


Trp Trp Cys Glu Tyr Arg Thr Ala Glu Ile Asp Glu Ser Arg Phe Lys 
    530                 535                 540                 


Ile Pro Cys Tyr Cys His Pro His Pro Phe Lys His Pro Val Tyr Val 
545                 550                 555                 560 


Glu Tyr Gly Lys Ser Asn Pro Lys Val Ile Phe Ala Met Lys Asn Asn 
                565                 570                 575     


Lys Val Lys Lys Gly His Ile Glu His Gly Trp Asn Pro Gln Asn Pro 
            580                 585                 590         


Arg Ser Ile Ala Leu Ser Leu Phe Asn Asn Gly Asn Arg Glu Ser Ser 
        595                 600                 605             


Leu Val Pro Phe Ile Trp Glu Ser Lys Arg Leu Trp Lys Asp Leu Gly 
    610                 615                 620                 


Gly Glu Ala Thr Gln Ile Gly Asp Ile Pro Arg Ser Asp Arg Met Gly 
625                 630                 635                 640 


Leu Ser Gly Lys Arg Glu Ser Val Lys Pro Lys Ala Pro Phe Gln Lys 
                645                 650                 655     


Glu Val Trp Asn Ala Arg Leu Gln Ser Asp Arg Arg Thr Leu Glu Lys 
            660                 665                 670         


Leu Glu Lys Tyr Trp Asn Pro Glu Ser Met Lys Trp Ile Asp Asp Gly 
        675                 680                 685             


Lys Phe Leu Ile Gln Ser Lys Trp Phe Ile Thr Phe Gly Pro Asp Met 
    690                 695                 700                 


Glu Thr Ala Glu Gly Pro Trp Lys Leu Tyr Leu Lys Glu Lys Tyr Val 
705                 710                 715                 720 


Asp Asn Lys Ile Leu Gly Asn Arg Ser Lys Glu Asn Gln Lys Arg Gly 
                725                 730                 735     


Tyr Arg Ala Lys Lys Leu Leu Ser Gly Tyr Pro Ala Gly Met Arg Ile 
            740                 745                 750         


Leu Ser Val Asp Leu Gly His Arg Tyr Ala Ala Ser Cys Ala Val Trp 
        755                 760                 765             


Glu Thr Ile Thr Lys Lys Gln Ile Thr Glu Glu Leu Ala Tyr Gln Pro 
    770                 775                 780                 


Asp Asn Asn Ser Leu Phe Glu His Ser Cys Lys Thr Ile Asp Lys Lys 
785                 790                 795                 800 


Ile Lys Asn Thr Val Tyr Arg Arg Ile Gly Glu Asp Ser Ile Asp Ala 
                805                 810                 815     


Pro Trp Ala Lys Leu Glu Lys Gln Phe Thr Ile Lys Leu Gln Gly Glu 
            820                 825                 830         


Asp Lys Ser Cys Tyr Leu Leu Arg Ser Asp Glu Lys Glu Leu Phe Arg 
        835                 840                 845             


Ser Ile Leu Ser Lys Leu Ser Cys Leu Asn Asn Asp Thr Gly His Asn 
    850                 855                 860                 


Ile Leu Glu Met Ile Glu Asn Leu Leu Arg Ile Val Lys Ala Lys Ile 
865                 870                 875                 880 


Tyr Arg Gln Gly Ile Leu Ala Arg Ile Ser Tyr Ser Met Thr Ala Gln 
                885                 890                 895     


Tyr Lys Pro Gly Lys Gly Gly Gln Lys Ser Pro Leu Ser Asp Glu Asp 
            900                 905                 910         


Lys Ile His Tyr Leu Ser Glu Asn Leu Ala Ala Trp Ser Ala Ile Met 
        915                 920                 925             


Gly Asn Gln Glu Trp Asn Glu Asp Val Ile Ser Asp Trp Tyr Lys Thr 
    930                 935                 940                 


Tyr Ile Ser His Leu Val Ser Gly Pro Lys Pro Lys Glu Gly Asn Arg 
945                 950                 955                 960 


Lys Ser Asp Arg Asp Lys Ile Ile Glu Tyr Phe Leu Pro Ala Ala Arg 
                965                 970                 975     


Lys Leu Tyr Asp Asp Asn Glu Thr Arg Ile Lys Ile His Asp Leu Phe 
            980                 985                 990         


Lys Glu Leu Trp Asp Glu Asn Asn  Lys Gln Leu Ser Ala  Val Leu Lys 
        995                 1000                 1005             


Glu Ile  Lys Lys Ile Ile Leu  Pro Lys Gly Ile Arg  Tyr Phe Asp 
    1010                 1015                 1020             


Lys Asn  Thr Asp Asn Pro Ser  Lys Trp Lys Asn Asn  Gln Ser Lys 
    1025                 1030                 1035             


Leu Lys  Gln Ile Thr His Arg  Gly Gly Leu Ser Met  Gln Arg Ile 
    1040                 1045                 1050             


Val Ala  Ile Glu Glu Tyr Tyr  Lys Leu Ala Lys Ala  Tyr Lys Asn 
    1055                 1060                 1065             


His Pro  Glu Pro Asp Asp Leu  Thr Lys Asn Ile Pro  Leu Pro Gly 
    1070                 1075                 1080             


Asp Asn  Ser Ser Ala Gly Phe  Asn Gln Arg Ile Arg  Asp Thr Leu 
    1085                 1090                 1095             


Glu Arg  Met Lys Glu Gln Arg  Val Lys Gln Ile Ala  Ser Arg Ile 
    1100                 1105                 1110             


Val Glu  Ser Ala Leu Gly Leu  Gly Ile Glu Gly Tyr  Lys Lys Arg 
    1115                 1120                 1125             


Pro Leu  Thr Pro Glu Asn Lys  Pro Cys Gln Ala Ile  Val Ile Glu 
    1130                 1135                 1140             


Asp Leu  Ser His Tyr Arg Pro  Asp Glu Leu Gln Thr  Arg Arg Glu 
    1145                 1150                 1155             


Asn Arg  Arg Leu Met Gln Trp  Ser Ser Ser Lys Val  Lys Lys Tyr 
    1160                 1165                 1170             


Leu Lys  Glu Ala Cys Glu Met  His Asp Val Arg Leu  Val Glu Ile 
    1175                 1180                 1185             


Ser Pro  Glu Tyr Thr Ser Arg  Gln Asp Ser Arg Thr  Gly Ala Ala 
    1190                 1195                 1200             


Gly Leu  Arg Cys Ile Asp Ile  Asn Ile Arg Glu Phe  Leu Lys Asp 
    1205                 1210                 1215             


Ser Ser  Arg Trp Gln Asn Lys  Ile Asn Thr Ile Gln  Lys Lys Pro 
    1220                 1225                 1230             


Ala Asn  Lys Lys Ser Asn Leu  Asp Gln Tyr Leu Ile  Glu Leu Asn 
    1235                 1240                 1245             


Glu Ser  Leu Gly Asn Lys Tyr  Lys Asp Lys Val Ile  Pro Ser Asp 
    1250                 1255                 1260             


Asn Phe  Val Arg Ile Pro Arg  Lys Gly Gly Asp Val  Phe Val Ser 
    1265                 1270                 1275             


Ser Ser  Lys Glu Ser Pro Val  Ser Lys Gly Ile Gln  Ala Asp Leu 
    1280                 1285                 1290             


Asn Ala  Ala Ala Asn Ile Gly  Leu Lys Ala Leu Leu  Asp Pro Asp 
    1295                 1300                 1305             


Trp Ala  Gly Ala Trp Trp Tyr  Ile Leu Ile Glu Val  Lys Ser Asn 
    1310                 1315                 1320             


His Val  Ile Pro Tyr Gly Glu  Lys Tyr Lys Gly Ser  Glu Cys Leu 
    1325                 1330                 1335             


Arg Ala  Trp Lys Phe Ser Gly  Leu Glu Asn Gln Val  Met Lys Asn 
    1340                 1345                 1350             


Asn Met  Asn Leu Trp Arg Asp  Leu Gln Ser Gln Phe  Ser Gly Glu 
    1355                 1360                 1365             


Asp Lys  Trp Met Ser Tyr Lys  Glu Tyr Asn Glu Leu  Thr Glu Lys 
    1370                 1375                 1380             


Arg Val  Ile Asn Ile Leu Arg  Glu Arg Ala Gly Leu  Glu Leu Ile 
    1385                 1390                 1395             


Lys Glu  
    1400 


<210>  2
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CR RNA FOR ENGINEERED NUCLEASE

<400>  2
gtttaagtag atactactga aaagaccgat ggacaca                                37


<210>  3
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRACRRNA FOR ENGINEERED NUCLEASE

<400>  3
gggaagggcc tatttcccag catgtgtctt cgcatttaat tgctttagca ctgggcatcg       60

ttctttttag tagt                                                         74


<210>  4
<211>  1375
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ENGINEERED NUCLEASE

<400>  4

Met Asn Arg Ile Tyr Gln Gly Arg Val Ser Lys Ile Glu Ile Lys Asp 
1               5                   10                  15      


Ser Glu Gly Asn Phe Arg Asn Val Pro Val Gly Ser Pro Asp Thr Cys 
            20                  25                  30          


Pro Leu Trp Arg His His Arg Ile Phe Gln Asp Ala Val Asn Tyr Tyr 
        35                  40                  45              


Leu Val Ala Leu Gly Ala Leu Ala Gly Thr Gly Ser Glu Asn Ala Phe 
    50                  55                  60                  


Val Gly Leu Gly Ser Lys Asp Arg Val Ile His Asp Leu Tyr Ser Arg 
65                  70                  75                  80  


Leu Phe Asp Ser Trp Glu Arg Phe Pro Arg Asp Met His Gly Ala Ser 
                85                  90                  95      


Ser Leu Arg Asp Ser Leu Arg Arg Thr Leu Pro Gly Leu Ser Glu Arg 
            100                 105                 110         


Ala Ser Leu Gln Asp Ala Phe Asp Ala Ile Leu Ser Gly Asn Glu Ala 
        115                 120                 125             


Asn Ala Arg Glu Arg Val Leu Ser Leu Leu Ser Leu Ile Gln Asp Leu 
    130                 135                 140                 


Gly Gly Asp Ile Gln Lys Gly Ser Lys Arg Tyr Phe Pro Phe Phe Cys 
145                 150                 155                 160 


Glu Pro Ala Thr Lys Ala Thr Phe Pro Arg Ala Arg Val Gly Leu Leu 
                165                 170                 175     


Lys Val Glu Gly Lys Asp Phe Val Pro Arg Leu Leu Trp Ser Ser Asp 
            180                 185                 190         


Leu Glu Ile Ala Pro Asp Gln Val Val Glu Gln Leu Lys Phe Glu Tyr 
        195                 200                 205             


Phe Ala Asn Pro Asn Glu Ser Val Gln Pro Ile Glu Gly Asn Glu Ala 
    210                 215                 220                 


Arg Val Arg Leu Ile Glu Ala Leu Asp Asn Pro Gln Leu Gly Ile Glu 
225                 230                 235                 240 


Leu Pro Ile Glu Ile Leu Ser Asp Leu Arg Lys Arg Val His Leu Ile 
                245                 250                 255     


Glu Thr Asp Ile Arg Ile Pro Arg Tyr Phe Phe Gly Gly Ala Gly Ala 
            260                 265                 270         


Glu Leu Arg Lys Phe Arg Leu Asp Leu Phe Leu Ile Ala Ala Tyr Val 
        275                 280                 285             


Thr Pro Asp Pro Ser Ile Leu Arg Ala Leu Arg Asn Ser Phe Lys Glu 
    290                 295                 300                 


Pro Ser Ala Ser Lys Ser Ser Lys Lys Lys Asp Glu Thr Glu Glu Val 
305                 310                 315                 320 


Glu Asn Leu Leu Arg Ser Leu Gly Asp Asp Pro Leu Ile Leu Ala Arg 
                325                 330                 335     


Gly Glu Arg Gly Phe Val Phe Pro Ser Phe Thr Ser Leu Pro Thr Trp 
            340                 345                 350         


Val Gly Ala Asn Ala Gln Lys Pro Ile Trp Arg Asp Phe Asp Ile Ala 
        355                 360                 365             


Ala Phe Ala Glu Ala Leu Lys Ser Leu Asn Gln Phe Thr Ala Lys Thr 
    370                 375                 380                 


Glu Glu Arg Glu Glu Lys Leu Lys Lys Ala Glu Glu Thr Leu His Tyr 
385                 390                 395                 400 


Met Leu Gly Ile Ser Asp Ala Ile Pro Arg Ser Ser Asp Ser Glu Thr 
                405                 410                 415     


Glu Glu Gln Ala Pro Ser Arg Pro Gly Lys Asp Pro Arg Trp Pro Leu 
            420                 425                 430         


Val Ala Gln Leu Glu Lys Glu Leu Gly Glu Asn Leu Ser Glu Gly Thr 
        435                 440                 445             


Trp Gln Leu Ser Arg Ser Ala Met Arg Gly Leu Arg Asp Ile Ile Gly 
    450                 455                 460                 


Leu Trp Arg Lys His Pro Gly Ala Ser Val Val Thr Leu Gln Lys Asp 
465                 470                 475                 480 


Val Lys Thr Tyr Gln Ala Asp Glu Lys His Lys Arg Glu Ile Gly Ser 
                485                 490                 495     


Val Gln Leu Phe Leu Leu Leu Cys Glu Glu Arg Tyr His Ala Leu Trp 
            500                 505                 510         


Gln Thr Glu Thr Asp Asp Glu Arg Gly Asp Glu Ser Glu Glu Asn Asp 
        515                 520                 525             


Asp Pro Ala Arg Ile Leu Ser Asp Ala Ile Glu Val His Gln Ile Arg 
    530                 535                 540                 


Arg Glu Val Glu Arg Phe Arg Glu Pro Ile Arg Leu Thr Pro Ala Glu 
545                 550                 555                 560 


Pro Val Phe Ser Arg Arg Leu Phe Met Phe Ser Asp Leu Thr Asp Lys 
                565                 570                 575     


Leu Ala Lys Val Lys Phe Gly Glu Thr Thr Glu Glu Asn Ser Glu Val 
            580                 585                 590         


Lys Ser Gln Phe Val Glu Ala Ala Ile Ala Leu Lys Glu Gly Glu Asn 
        595                 600                 605             


Leu Lys Glu Ala Arg Val Arg Ile Thr Phe Ser Ala Pro Arg Leu His 
    610                 615                 620                 


Arg Asp Glu Leu Leu Gly Gly Ala Glu Ser Arg Trp Leu Gln Pro Ile 
625                 630                 635                 640 


Thr Ala Ala Leu Gly Phe Ser Asn Pro Ala Pro Ser Val Lys Phe Asp 
                645                 650                 655     


Ser Ala Val Ala Leu Met Pro Asp His Met Asp Asp Gly Arg Ile Arg 
            660                 665                 670         


His Leu Leu Asn Phe Pro Val Asn Phe Asp Ser Ala Trp Leu His Gln 
        675                 680                 685             


Ser Ile Gly Lys Ala Asp Leu Trp Lys Ser Gln Phe Asn Gly Thr Lys 
    690                 695                 700                 


Asp Lys Asn Leu His Leu His Trp Ala Gly Thr Ala Arg Asp Thr Thr 
705                 710                 715                 720 


Arg Lys Asn Thr Trp Trp Glu Asn Arg Thr Ile Ile Glu Asn Gly Phe 
                725                 730                 735     


Thr Val Leu Ser Asn Asp Leu Gly Gln Arg Ser Ala Gly Ala Trp Ala 
            740                 745                 750         


Leu Leu Lys Val Thr Cys Ser Arg Pro Asp Thr Lys His Pro Val Arg 
        755                 760                 765             


Ser Ile Gly His Asp Gly Thr Arg Glu Trp Phe Ala Thr Val Leu Ala 
    770                 775                 780                 


Thr Gly Ile His Arg Leu Pro Gly Glu Asp Gln Arg Ile Leu Lys Asn 
785                 790                 795                 800 


Gly Lys Trp Ala Thr Glu Gln Ser Gly Lys Lys Gly Arg Asn Ala Thr 
                805                 810                 815     


Phe Ser Glu Tyr Glu Ala Ala Cys Val Leu Ala Lys Asn Leu Gly Cys 
            820                 825                 830         


Glu Ser Val Glu Asn Trp Leu Gly Met Ser Gly Glu Lys Ser Tyr Pro 
        835                 840                 845             


Ala Leu Asn Asp Gln Leu Val Lys Ile Ala Asn Arg Arg Ile Thr Arg 
    850                 855                 860                 


Leu Gly Thr Tyr His Arg Trp Ser Cys Phe Ser Pro Glu Lys Phe Glu 
865                 870                 875                 880 


Asp Pro Ala Arg Arg Ala Asn Val Ile Gly Gly Gln Leu Ala Glu Leu 
                885                 890                 895     


Ser Ala Tyr Gln Asp Glu Asn Val Thr Val Ser Ala Asp Ile Leu Lys 
            900                 905                 910         


Ser Gly Asp Phe Glu Gly Phe Arg His Arg Ala Gly Ala Ala Phe Glu 
        915                 920                 925             


Ala Leu Arg Thr Glu Leu Glu Val His Leu Val Asn Leu Ala Asn Leu 
    930                 935                 940                 


Thr Ala Pro Leu Arg Gln Lys Val Trp Ser Trp Gln Lys Arg Pro Asp 
945                 950                 955                 960 


Ser Ser Gly Tyr Gly Asp Leu Leu Met Val Asp Leu Asp Asp Cys His 
                965                 970                 975     


Pro Lys Ile Arg Gly Gln Arg Gly Leu Ser Met Ala Arg Leu Glu Gln 
            980                 985                 990         


Leu Glu Gly Leu Arg Arg Leu Phe  Leu Arg Tyr Asn Arg  Ser Leu Asp 
        995                 1000                 1005             


Arg Ser  Pro Gly Ile Pro Ala  Lys Phe Gly Arg Glu  Asp Val Gly 
    1010                 1015                 1020             


Arg Thr  Ser Gly Glu Pro Cys  Gln Ala Leu Leu Val  Lys Ile Asp 
    1025                 1030                 1035             


Arg Met  Lys Glu Gln Arg Val  Asn Gln Thr Ala His  Leu Ile Leu 
    1040                 1045                 1050             


Ala Gln  Ala Leu Gly Val Arg  Leu Cys Pro His Arg  Ile Glu Glu 
    1055                 1060                 1065             


Asn Glu  Arg Lys Ser Arg Asp  Leu His Gly Glu Tyr  Glu Lys Ile 
    1070                 1075                 1080             


Pro Gly  Arg Glu Pro Val Asp  Phe Ile Val Ile Glu  Asp Leu Ser 
    1085                 1090                 1095             


Arg Tyr  Leu Ser Ser Gln Gly  Arg Ala Pro Ser Glu  Asn Ser Arg 
    1100                 1105                 1110             


Leu Met  Lys Trp Ala His Arg  Ala Val Arg Asp Lys  Leu Lys Met 
    1115                 1120                 1125             


Leu Ala  Glu Glu Pro Phe Gly  Ile Pro Val Val Glu  Thr Val Pro 
    1130                 1135                 1140             


Ala Tyr  Ser Ser Arg Phe His  Ala Leu Asn Gly Gln  Ala Gly Ser 
    1145                 1150                 1155             


Arg Leu  His Glu Leu His Glu  Leu Glu Ala Tyr Gln  Gln Gln Ser 
    1160                 1165                 1170             


Leu Ile  Asn Leu Ala Ala Lys  Thr Asp Phe Gln Asn  Arg Asp Arg 
    1175                 1180                 1185             


Ser Lys  Ala Ala Gly Glu Leu  Phe Glu Gln Phe Gln  Ala Leu Ala 
    1190                 1195                 1200             


Lys Leu  Asn Glu Arg Arg Arg  Ala Glu Gly Lys Lys  Val Pro Arg 
    1205                 1210                 1215             


Thr Leu  Tyr Tyr Pro Lys Ser  Gly Gly Pro Leu Phe  Leu Ala Ser 
    1220                 1225                 1230             


Arg Asp  Gly Asp Thr Ile His  Ala Asp Val Asn Ala  Ala Ile Asn 
    1235                 1240                 1245             


Leu Gly  Leu Arg Ala Ile Ala  Ala Pro Ala Cys Ile  Asp Ile His 
    1250                 1255                 1260             


Arg Arg  Leu Arg Ala Thr Lys  Glu Lys Glu Val Tyr  Arg Pro Arg 
    1265                 1270                 1275             


Val Gly  Asn Ala Arg Glu Lys  Ser Ala Phe Ser Lys  Asp Asp Ile 
    1280                 1285                 1290             


Ile Gln  Pro Ser Gly Ala Pro  Ser Lys Lys Phe Ala  Ser Ser Ser 
    1295                 1300                 1305             


Ser Pro  Asn Phe Phe Tyr Glu  Pro Glu Asp Leu Lys  Gln Ala Asn 
    1310                 1315                 1320             


Gly Glu  Pro Leu Phe Asp Arg  Ala Met Phe Gly Glu  Tyr Ser Leu 
    1325                 1330                 1335             


Val Ser  Gly Val Ser Leu Trp  Ser Met Val Asn Asn  Ala Ile Tyr 
    1340                 1345                 1350             


Ile Arg  Cys Val Glu Leu Asn  Arg Thr Arg Leu His  Gly Lys Asp 
    1355                 1360                 1365             


Pro Asp  Asp Gln Ile Pro Met  
    1370                 1375 


<210>  5
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRRNA FOR ENGINEERED NUCLEASE

<400>  5
gccgcagccc ccgcgatggg aaagagattg tggcgg                                 36


<210>  6
<211>  97
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRACR RNA FOR ENGINEERED NUCLEASE

<400>  6
gccccgattt ccctttgaat gatctcggcc tcgttgccac tgaccgaatt cttccgcctt       60

tggaattcca agctctttga catcgcgagc gctgagg                                97


<210>  7
<211>  1397
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ENGINEERED NUCLEASE

<400>  7

Met Ala Ala Phe Gln Arg Ser Tyr Thr Met Asn Leu Lys Pro Ala Thr 
1               5                   10                  15      


Ser Glu Gln Asp Lys Phe Ile Leu Trp Asn Arg Leu Phe Leu Thr His 
            20                  25                  30          


Trp Ser Val Asn Glu Gly Ala Lys Ile Phe Gly Glu Leu Phe Leu Asn 
        35                  40                  45              


Leu Arg Gly Gly Leu Ser Pro Glu Leu Gly Ile Phe Asp Leu Asn Lys 
    50                  55                  60                  


Asp Lys Asp Asp Arg Lys Lys Lys Ala Leu Val Met Gly Arg Arg Arg 
65                  70                  75                  80  


Leu Leu Ala Leu Gly Trp Leu Ser Val Glu Asp Asn Leu Ser Ala Gly 
                85                  90                  95      


Asp His Pro Phe Arg Ile Arg Glu Ile Pro Val Gly Arg Asn Met Glu 
            100                 105                 110         


Ile Lys Gln Ala Thr Thr Leu Leu Thr Glu Ile Leu Lys Asn Lys Gly 
        115                 120                 125             


Val Lys Asp Glu Ala Val Ile Lys Glu Trp Ile Asp Asp Cys Thr Pro 
    130                 135                 140                 


Ser Leu Ile Ala Asn Ile Arg Glu Asp Ala Val Trp Ile Asn Arg Ala 
145                 150                 155                 160 


Lys Ser Phe Tyr Ser Met Asn Pro Cys Pro Thr Lys Asp Glu Val Trp 
                165                 170                 175     


Lys Ile Leu Ser Tyr Val Leu Asn Thr Ser Phe Leu Asp Leu Ser Leu 
            180                 185                 190         


Asn Asp Ser Ser Glu Arg Asp Asn Thr Lys Asn Lys Lys Gly Thr Lys 
        195                 200                 205             


Glu Asn Glu Lys Asp Val Ser Asn Lys Ser Lys Glu Leu Tyr Gly Trp 
    210                 215                 220                 


Leu Phe Thr Lys Asn Pro Asn Lys Met Arg Glu Ala Gly Glu Asn Lys 
225                 230                 235                 240 


Asp Lys Phe Ile Asn Asn Phe Arg Glu Asn Phe Asn Thr Phe Thr Asp 
                245                 250                 255     


Tyr Ala Asn Leu Lys Val Glu Ile Glu Leu Trp Arg Lys Asn Asn Ile 
            260                 265                 270         


Ser Asn Thr Leu Leu Ile Thr Gln Lys Ala Lys Tyr Pro Pro Glu Val 
        275                 280                 285             


Lys Glu Ala Asn His Pro Ser Lys Phe Ser Val Gly Tyr Arg Lys Leu 
    290                 295                 300                 


Leu Val His Leu Glu Leu Trp Pro Ser Ser Lys Asp Glu Asn Gly Asp 
305                 310                 315                 320 


Ile Pro Lys Gly Ile Glu Gly Lys Asp Lys Ser His Asn Gln Ile Leu 
                325                 330                 335     


Leu Asp Tyr Leu Leu Glu Val Cys Asn Glu Gly Asn Lys Thr Thr Lys 
            340                 345                 350         


Lys Val Ile Val Pro Ala Trp Ala Asp Gly Ile Lys Thr Glu Leu Glu 
        355                 360                 365             


Ser Lys Ala Ser Ile Lys Val Gly Asp Ser Thr Ser Ser Val Leu Gln 
    370                 375                 380                 


Arg Leu Met Ile Lys Met Ala Ala Arg Arg Ile Ser Gln Thr Leu Ser 
385                 390                 395                 400 


Trp Ile Lys Ile Asn Glu Gln Val Arg His Asp Ala Tyr Gln Lys Lys 
                405                 410                 415     


Asn Lys Ala Phe Lys Leu Leu Cys Glu Ile Asp Lys Asn Gly Glu Ala 
            420                 425                 430         


Cys Lys Trp Leu Glu Asn Tyr Glu Leu Phe Arg Arg Asp Asp Ser Gly 
        435                 440                 445             


Gly Glu Glu Tyr His Ile Ser Ala Arg Ala Ile Ser Cys Trp Lys Gln 
    450                 455                 460                 


Ile Leu Glu Glu Trp Gln Lys Asn Asp Ser Ser Lys Ala Leu Arg Glu 
465                 470                 475                 480 


Lys Val Lys Val Val Gln Ala Ala Glu Asp Lys Phe Gly Asp Ala Arg 
                485                 490                 495     


Leu Phe Glu Asp Leu Ala Asp Asp Asn Ala Arg Ser Val Trp Leu Leu 
            500                 505                 510         


Pro Asp Gly Asn Lys Thr Pro Asp Ile Leu Asn Trp Trp Cys Glu Tyr 
        515                 520                 525             


Arg Thr Ala Asp Ile Asp Glu Ser Arg Phe Lys Ile Pro Cys Tyr Cys 
    530                 535                 540                 


His Pro His Pro Phe Lys His Pro Val Tyr Val Glu Tyr Gly Lys Ser 
545                 550                 555                 560 


Asn Pro Gln Val Ile Phe Ser Leu Lys His Asp Lys Ala Arg Lys Asn 
                565                 570                 575     


Arg Ile Asp Asn Gly Trp Asn Pro Lys Asn Pro Arg Ile Leu Ala Leu 
            580                 585                 590         


Leu Leu Leu Asp Ile Val Arg Gln Lys Ser Thr Leu Ala Pro Phe Val 
        595                 600                 605             


Trp Glu Ser Lys Arg Leu Trp Lys Asp Leu Gly Gly Asp Ala Thr Val 
    610                 615                 620                 


Thr Tyr Lys Ile Pro Arg Ser Asp Arg Met Gly Leu Ser Ser Ile Gly 
625                 630                 635                 640 


Asn Ile Asp Tyr Ala Arg Pro Glu Val Pro Phe Leu Lys Glu Lys Trp 
                645                 650                 655     


Asn Ala Arg Leu Gln Ser Asp Arg Arg Thr Leu Glu Lys Leu Glu Lys 
            660                 665                 670         


Tyr Trp Asn Pro Glu Ser Met Lys Trp Ile Asp Asp Gly Lys Phe Leu 
        675                 680                 685             


Ile Gln Ser Lys Trp Phe Ile Thr Phe Gly Pro Asp Met Glu Thr Ala 
    690                 695                 700                 


Glu Gly Pro Trp Lys Leu Tyr Leu Lys Glu Asn Ile Asn Asp Asn Asn 
705                 710                 715                 720 


Tyr Leu Gly Asn Arg Ser Lys Glu Asn Gln Lys Arg Gly Tyr Arg Ala 
                725                 730                 735     


Lys Lys Leu Leu Ser Gly Tyr Pro Ala Gly Met Arg Ile Leu Ser Val 
            740                 745                 750         


Asp Leu Gly His Arg Tyr Ala Ala Ser Cys Ala Ile Trp Glu Thr Ile 
        755                 760                 765             


Thr Lys Lys Gln Ile Thr Glu Glu Leu Ala Tyr Gln Pro Asp Lys Asn 
    770                 775                 780                 


Ser Val Phe Glu His Ser Cys Lys Thr Ile Asp Lys Lys Ile Lys Asn 
785                 790                 795                 800 


Thr Val Tyr Arg Arg Ile Gly Asp Asp Ser Ile Asp Ala Pro Trp Ala 
                805                 810                 815     


Lys Leu Glu Lys Gln Phe Thr Ile Lys Leu Gln Gly Glu Asp Lys Ser 
            820                 825                 830         


Cys Tyr Leu Leu Arg Ser Asp Glu Lys Glu Leu Phe Arg Ser Ile Leu 
        835                 840                 845             


Ser Lys Leu Ser Cys Leu Asn Asn Asp Thr Gly His Asn Ile Leu Glu 
    850                 855                 860                 


Met Ile Glu Asn Leu Leu Arg Ile Val Lys Ala Lys Ile Tyr Arg Gln 
865                 870                 875                 880 


Gly Ile Leu Ala Arg Ile Ser Tyr Ser Met Thr Ala Gln Tyr Lys Pro 
                885                 890                 895     


Gly Lys Gly Gly Gln Lys Ser Pro Leu Ser Asp Glu Asp Lys Ile His 
            900                 905                 910         


Tyr Leu Ser Glu Asn Leu Ala Ala Trp Ser Ala Leu Met Gly Asn Gln 
        915                 920                 925             


Glu Trp Asn Glu Asp Gly Ile Ser Asp Trp Tyr Lys Lys Tyr Ile Ser 
    930                 935                 940                 


His Leu Val Ser Gly Pro Lys Pro Lys Glu Gly Asn Arg Lys Ser Asp 
945                 950                 955                 960 


Arg Asp Lys Ile Ile Glu Tyr Phe Leu Pro Ala Ala Arg Lys Leu Tyr 
                965                 970                 975     


Asp Asp Asn Glu Thr Arg Ile Asn Ile His Asp Leu Phe Lys Glu Leu 
            980                 985                 990         


Trp Asp Glu Asn Asn Lys Gln Leu  Ser Ala Val Leu Lys  Glu Ile Lys 
        995                 1000                 1005             


Lys Ile  Ile Leu Pro Lys Gly  Ile Arg Tyr Phe Asp  Lys Asn Asn 
    1010                 1015                 1020             


Asp Ser  Ser Ser Arg Trp Lys  Asn Asn Gln Ser Lys  Leu Lys Gln 
    1025                 1030                 1035             


Ile Thr  His Arg Gly Gly Leu  Ser Leu Arg Arg Ile  Val Ala Ile 
    1040                 1045                 1050             


Glu Gly  Tyr Tyr Lys Leu Ala  Lys Ala Tyr Lys Asn  His Pro Glu 
    1055                 1060                 1065             


Pro Asp  Asn Leu Thr Lys Asn  Ile Pro Leu Pro Gly  Asp Asn Ser 
    1070                 1075                 1080             


Ser Ala  Gly Phe Asn Gln Arg  Ile Arg Asp Thr Leu  Glu Arg Met 
    1085                 1090                 1095             


Lys Glu  Gln Arg Val Lys Gln  Ile Ala Ser Arg Ile  Val Glu Ser 
    1100                 1105                 1110             


Ala Leu  Gly Leu Gly Ile Glu  Gly Tyr Lys Lys Arg  Pro Leu Thr 
    1115                 1120                 1125             


Pro Glu  Ser Lys Pro Cys Gln  Ala Ile Val Ile Glu  Asp Leu Ser 
    1130                 1135                 1140             


His Tyr  Arg Pro Asp Glu Leu  Gln Thr Arg Arg Glu  Asn Arg Arg 
    1145                 1150                 1155             


Leu Met  Gln Trp Ser Ser Ser  Lys Val Lys Lys Tyr  Leu Ser Glu 
    1160                 1165                 1170             


Ala Cys  Glu Met His Asp Val  Leu Leu Val Glu Ile  Ser Pro Glu 
    1175                 1180                 1185             


Tyr Thr  Ser Arg Gln Asp Ser  Arg Thr Gly Val Ala  Gly Leu Arg 
    1190                 1195                 1200             


Cys Ile  Asp Ile Asn Ile Arg  Glu Phe Leu Lys Asp  Ser Ser Ser 
    1205                 1210                 1215             


Trp Gln  Asn Lys Ile Lys Thr  Ile Gln Met Lys Pro  Thr Asn Lys 
    1220                 1225                 1230             


Lys Ser  Asn Leu Asp Gln Tyr  Leu Ile Glu Leu Asn  Glu Ser Leu 
    1235                 1240                 1245             


Gly Glu  Arg Tyr Lys Asp Lys  Val Ile Pro Ser Asp  Lys Phe Val 
    1250                 1255                 1260             


Arg Ile  Pro Arg Lys Gly Gly  Asp Ile Phe Val Ser  Ser Ser Lys 
    1265                 1270                 1275             


Glu Ser  Pro Val Ser Lys Gly  Ile Gln Ala Asp Leu  Asn Ala Ala 
    1280                 1285                 1290             


Ala Asn  Ile Gly Leu Lys Ala  Leu Leu Asp Pro Asp  Trp Ala Gly 
    1295                 1300                 1305             


Ala Trp  Trp Tyr Ile Leu Ile  Glu Ala Lys Ser Asn  His Val Ile 
    1310                 1315                 1320             


Pro Tyr  Gly Lys Lys Tyr Lys  Gly Ala Glu Cys Leu  Arg Asp Phe 
    1325                 1330                 1335             


Lys Phe  Ser Gly Leu Glu Asn  Gln Val Met Lys Asn  Asn Met Asn 
    1340                 1345                 1350             


Leu Trp  Arg Asp Leu Gln Ser  Gln Phe Ser Ser Glu  Asp Lys Trp 
    1355                 1360                 1365             


Met Ser  Tyr Lys Glu Tyr Asn  Glu Leu Thr Glu Lys  Arg Val Ile 
    1370                 1375                 1380             


Asn Ile  Leu Arg Glu Arg Ala  Gly Leu Glu Leu Ile  Glu Glu 
    1385                 1390                 1395         


<210>  8
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CR RNA FOR ENGINEERED NUCLEASE

<400>  8
gtttaagtag atactactaa aaagaccgat ggacac                                 36


<210>  9
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRACR RNA FOR ENGINEERED NUCLEASE

<400>  9
gggaagggcc tatttcccag catgtgtctt cgcatttaat tgctttagca ctgggcatcg       60

ttctttttag tagt                                                         74


<210>  10
<211>  1097
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ENGINEERED NUCLEASE

<400>  10

Met Val Thr Arg Ala Leu Asn Leu Lys Leu Val Val Pro Arg Arg Pro 
1               5                   10                  15      


Gly Glu Leu Thr Lys Ala Glu Ala Leu Trp Ser Thr His Asp Ile Val 
            20                  25                  30          


Asn Arg Ala Thr Ser Tyr Tyr Glu Ser Gln Leu Leu Leu Cys Arg Gln 
        35                  40                  45              


Gln Asp Tyr Gln Thr Arg Glu Leu Thr Val Ser Ala Gly Asp Gln Ala 
    50                  55                  60                  


Pro Asp Leu Asp Ala Leu Ile Ala Asn Ala Arg Asp Arg Asn Arg Tyr 
65                  70                  75                  80  


Arg Gly Leu Glu Lys Pro Gln Val Val Arg Glu Lys Leu Arg Asn Leu 
                85                  90                  95      


Tyr Glu Ala Ile Val Pro Pro Ala Ile Gly Lys Thr Gly Thr Ala Gln 
            100                 105                 110         


Ala Val Gly Ala Phe Val Ser Pro Leu Leu Asp Ala Asp Ser Arg Gly 
        115                 120                 125             


Phe Thr Glu Ile Phe Asp Lys Ile Glu Ala Leu Pro Asn Trp Val Asp 
    130                 135                 140                 


Gly Val Arg Ala Glu Glu Pro Asp Ala Leu Glu Ala Ala Ala Asp Trp 
145                 150                 155                 160 


Leu Lys Ser Pro Gln Gly Lys Glu Arg Leu Arg Pro Thr Gly Ala Pro 
                165                 170                 175     


Pro Thr Trp Ile Lys Leu Ala Lys Lys Lys Asp Ala Gly Trp Ala Ala 
            180                 185                 190         


Ala Phe Val Ala Asp Ile Asp Lys Lys Leu Lys Glu Val Glu Gly Thr 
        195                 200                 205             


Pro Thr Leu Met Gln Glu Leu Arg Ala Leu Gly Val Met Pro Leu Phe 
    210                 215                 220                 


Pro Ser Phe Phe Ala Ser Arg Ile Ala Gly His Lys Gly Ala Val Ser 
225                 230                 235                 240 


Thr Trp Asp Arg Leu Ala Leu Arg Leu Ala Val Ala His Leu Leu Ser 
                245                 250                 255     


Trp Glu Ser Trp Val Glu Leu Ala Ala Lys Glu His Ala Ala Arg Val 
            260                 265                 270         


Ala Lys Leu Glu Lys Phe Arg Asp Asp Asn Ile Leu Gly Glu Ile Ala 
        275                 280                 285             


Asp Ala Val Glu Ala Leu Arg Leu Tyr Glu Lys Glu Arg Thr Glu Glu 
    290                 295                 300                 


Leu Gln Gln Lys Ala Gln Leu Asp Ala Glu Glu Val Arg Thr Thr Ser 
305                 310                 315                 320 


Arg Thr Ile Arg Gly Trp Val Asp Leu Arg Glu Lys Trp Leu Lys Thr 
                325                 330                 335     


Asp Ala Ser Pro Asp Ala Leu Ile Ser Leu Val Ala Ala Glu Gln Lys 
            340                 345                 350         


Arg Lys Ser Gly Lys Phe Gly Asp Pro Gln Leu Phe Arg Trp Leu Ala 
        355                 360                 365             


Lys Pro Glu Asn His Phe Val Trp Asn Lys Pro Asp Phe Asp Pro Pro 
    370                 375                 380                 


Ser Leu Phe Ala Ser Leu Arg Met Ile Glu Gly Leu Val Glu Arg Ser 
385                 390                 395                 400 


Lys Glu Thr Ala Trp Met Thr Leu Pro Asp Ala Arg Leu His Pro Arg 
                405                 410                 415     


Ser Ser Gln Trp Glu Pro His Gly Gly Gly Asn Leu Lys Thr Phe Arg 
            420                 425                 430         


Leu Glu Gln Gly Glu Gly Gly Ser Leu Ser Val Thr Leu Pro Leu Leu 
        435                 440                 445             


Arg Lys Ser Gly Asp Asp Ser Tyr Val Glu Glu Glu His Ala Phe Ser 
    450                 455                 460                 


Leu Ala Gly Ser Lys Gln Ile Pro Asn Ala Ser Leu Asp Val Arg Arg 
465                 470                 475                 480 


Asn Lys Tyr Cys Leu Ser Tyr Arg Thr Pro Thr Gly Glu Glu Ala Glu 
                485                 490                 495     


Ala Val Val Gly Ser Ala Asp Leu Leu Leu Asp Trp Tyr Phe Leu Gln 
            500                 505                 510         


Gln Arg Ser Glu His Arg Pro Glu Glu Gly Asp Ile Gly Pro Ala Phe 
        515                 520                 525             


Leu Lys Leu Ala Leu Asp Ile Thr Pro Ile Asp Pro Val Trp Gly Glu 
    530                 535                 540                 


Arg Glu Lys Thr Pro Ala Ile His His Phe Lys Thr Ala Ser Gly Lys 
545                 550                 555                 560 


Asn Thr Arg His Ala Asp Gly Val Ala Pro Gly Phe Arg Met Leu Ala 
                565                 570                 575     


Val Asp Leu Gly Ile Arg Thr Leu Ala Thr Cys Ser Val Phe Glu Leu 
            580                 585                 590         


Lys Ala Thr Ala Pro Ala Gly Arg Leu Ser Phe Pro Ile Ala His Leu 
        595                 600                 605             


Asp Leu His Ala Val His Glu Arg Ser Phe Thr Leu Thr Leu Asp Gly 
    610                 615                 620                 


Glu Asp Pro Asp Arg Asp Ala Glu Arg Trp Arg Glu Asn Lys Ser Ala 
625                 630                 635                 640 


Glu Leu Arg Arg Leu Arg Met Gly Leu Thr Arg Tyr Arg Asn Ile Arg 
                645                 650                 655     


Asn Met Arg Glu Asp Ala Pro Asp Glu Arg Glu Val Leu Leu Glu Asp 
            660                 665                 670         


Leu Gln Glu Lys Val Gln Glu His Gly Trp Ala Phe Glu Glu Pro Leu 
        675                 680                 685             


Leu Arg Glu Leu Ala Lys His Lys Asp Thr Pro Glu Pro Ile Trp Glu 
    690                 695                 700                 


Ala Glu Leu Thr Lys Ala Leu Ala Gln Phe Arg Ser Asp Phe Gly Val 
705                 710                 715                 720 


Ile Val Gly Glu Trp Arg Arg Ser Asn Arg Ala Arg Ser Thr Asp Ser 
                725                 730                 735     


His Ala Gly Lys Ser Met Trp Ala Ile Asp His Leu Thr Asn Ser Arg 
            740                 745                 750         


Arg Phe Leu Met Ser Trp Ser Leu Leu Ser Lys Pro Gly Gln Ile Arg 
        755                 760                 765             


Arg Leu Asp Arg Asp Lys Gln Gly Val Phe Ala Lys His Leu Leu Asp 
    770                 775                 780                 


His Leu Glu Gly Leu Lys Ala Asp Arg Leu Lys Thr Gly Ser Asp Leu 
785                 790                 795                 800 


Ile Val Gln Ala Ala Arg Gly Phe Arg Arg Asp Lys Arg Gly Asn Trp 
                805                 810                 815     


His Lys Ala Tyr Lys Pro Cys His Gly Ile Leu Phe Glu Asp Leu Ser 
            820                 825                 830         


Arg Tyr Arg Met Arg Thr Asp Arg Pro Arg Arg Glu Asn Ser Gln Leu 
        835                 840                 845             


Met Lys Trp Ala His Arg Ala Val Pro Lys Glu Val Gly Met Gln Ala 
    850                 855                 860                 


Glu Val Tyr Gly Ile Arg Val Glu Asp Thr Gly Ala Ala Phe Ser Ser 
865                 870                 875                 880 


Arg Phe His Ala Ala Ser His Thr Pro Gly Ile Arg Met His Pro Ile 
                885                 890                 895     


Cys Gln Lys Asp Leu Glu Asn Glu Trp Leu Leu Asp Glu Ile Glu Lys 
            900                 905                 910         


Gln Asn Ser Gly Val Lys Arg Arg Glu Leu Lys Leu Gly Gln Leu Val 
        915                 920                 925             


Gln Leu Asn Gly Gly Glu Leu Phe Ala Cys Val Thr Ala Ser Gly Val 
    930                 935                 940                 


Lys Thr Leu His Ala Asp Ile Asn Ala Ala Gln Asn Leu Gln Arg Arg 
945                 950                 955                 960 


Phe Phe Thr Arg His Gly Asp Ala Phe Arg Ile Val Ala Arg Lys Val 
                965                 970                 975     


Leu Val Asp Glu Glu Glu Val Trp Val Pro Arg Ser Leu Gly Lys Arg 
            980                 985                 990         


Leu Leu Gly Ala Leu Gly Ser His  Gly Lys Leu Val Pro  Thr Gly His 
        995                 1000                 1005             


Glu Ser  Gly Ser Cys Arg Phe  Glu Glu Ile Thr Thr  Arg Ala Trp 
    1010                 1015                 1020             


Ser Lys  Leu Ser Gly Glu Lys  Leu Ser Asp Asp Arg  Val Gly Asn 
    1025                 1030                 1035             


Glu Glu  Asp Gln Ile Ile Ala  Ser Ile Glu Glu Glu  Ala Leu Glu 
    1040                 1045                 1050             


Arg Thr  Gly Glu Val Val Val  Phe Phe Arg Asp Pro  Ser Gly Gln 
    1055                 1060                 1065             


Val Leu  Pro Arg Asp Leu Trp  Tyr Pro Ser Lys Thr  Phe Trp Ser 
    1070                 1075                 1080             


Ile Val  Lys Ser Thr Thr Leu  Ser Lys Leu Lys Ala  Ala Pro 
    1085                 1090                 1095         


<210>  11
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CR RNA FOR ENGINEERED NUCLEASE

<400>  11
ggtgaagtca cccccgtttt gtaggcctac tggcac                                 36


<210>  12
<211>  98
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRACR RNA FOR ENGINEERED NUCLEASE

<400>  12
atgtaaccct ataggggttg cgtgagtcgg ccatagtgcc tcggcaacag cgtaaaaaac       60

tgctgccagt ggtcgaagta agtcaacaaa acggaggt                               98


<210>  13
<211>  1500
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ENGINEERED NUCLEASE

<400>  13

Met Ala Thr Ala Ile Asn Tyr Pro Thr Thr Gln Arg Ala Tyr Thr Leu 
1               5                   10                  15      


Arg Leu Arg Gly Ile Asp Pro Gln Asp Gln Ser Trp Arg Asp Ala Leu 
            20                  25                  30          


Trp Ala Thr His Glu Ala Val Asn Arg Gly Ala Lys Val Phe Gly Glu 
        35                  40                  45              


Trp Leu Leu Thr Leu Arg Gly Gly Leu Asp His Gln Leu Ala Asp Ala 
    50                  55                  60                  


Pro Val Lys Val Arg Gly Gly Thr Thr Arg Leu Pro Ser Asp Glu Glu 
65                  70                  75                  80  


Arg Arg Asp Arg Arg Val Leu Leu Ala Leu Ser Trp Leu Ser Val Glu 
                85                  90                  95      


Asp Ala His Gly Ala Pro Pro Asp Ala Ser Leu Ile Val Ala Lys Gly 
            100                 105                 110         


Thr Asp Ser Ala Asp Cys Arg Ala Arg Lys Leu Ala Asp Ala Leu Ile 
        115                 120                 125             


Ala Ile Leu Gln Ala Arg Ser Val Ala Ala Ser Glu Ile Gly Asp Pro 
    130                 135                 140                 


Ser Lys Pro Pro Glu Asp Gln Pro Gly Thr Trp Leu Gly Asp Cys Met 
145                 150                 155                 160 


Gly Ser Leu Ser Ala Ala Ile Arg Asp Asp Ala Val Trp Val Asn Arg 
                165                 170                 175     


Ser Lys Ala Phe Asp Ala Ala Thr Gln Ser Cys Pro Ser Leu Thr Arg 
            180                 185                 190         


Asp Glu Ile Trp Asp Phe Leu Glu Pro Phe Phe Ala Ser Pro Asp Ala 
        195                 200                 205             


Tyr Leu Lys Pro Glu Arg Ala Glu Ser Asp Glu Gly Asp Ser Thr Ser 
    210                 215                 220                 


Ala Ala Thr Glu Asp Lys Ala Lys Asp Leu Val Gln Lys Ala Gly Gly 
225                 230                 235                 240 


Trp Leu Ser Lys Arg Met Gly Ala Gly Gly Gly Ala Asn Phe Gln Asp 
                245                 250                 255     


Leu Ala Arg Ala Tyr Gln Ala Ile Ala Gln Trp Ala Ser Ser Ala Gln 
            260                 265                 270         


Pro Gly Gln Ser Ala Gln Gln Ala Val Gly Ser Leu Ala Gly Tyr Leu 
        275                 280                 285             


Ser Gln His Gly Phe Ser Pro Thr Ala Asn Asp Ala Thr Gly Val Leu 
    290                 295                 300                 


Ala Val Ile Ser Gly Pro Gly Tyr Lys Ser Ala Thr Arg Asn His Ile 
305                 310                 315                 320 


Thr Ala Ile Ala Thr Ser Pro Glu Ile Thr Pro Gln Asp Leu Ser Lys 
                325                 330                 335     


Leu Gln Glu Leu Ala Thr Lys Asp Lys Ala Gly Cys Ser Ser Lys Ile 
            340                 345                 350         


Gly Gly Lys Gly Pro Arg Pro Tyr Ala Thr Met Ile Leu Gln Gln Val 
        355                 360                 365             


Glu Ala Ala Cys Gly Phe Thr Tyr Leu Gln Ser Asp Gly Pro Ala Arg 
    370                 375                 380                 


His Arg Glu Phe Ser Val Met Leu Asp His Ala Ala Arg Arg Val Asn 
385                 390                 395                 400 


Val Ala His Ser Trp Ile Lys Asn Ala Glu Ala Glu Arg Arg Gln Phe 
                405                 410                 415     


Glu Ser Asp Ala Arg Arg Ile Lys Lys Val Pro Gln Asp Ala Leu Asn 
            420                 425                 430         


Trp Leu Arg Gly Tyr Cys Glu Glu Arg Gly Gly Ala Ser Gly Ser Leu 
        435                 440                 445             


Glu Gly Tyr Arg Ile Arg Arg Arg Ala Ile Asp Gly Trp Asp Gln Val 
    450                 455                 460                 


Val Ile Arg Trp Ser Arg Ser Asp Cys Gln Ser Ala Asp Asp Arg Ile 
465                 470                 475                 480 


Ala Ala Ala Arg Gln Leu Gln Asp Asp Pro Glu Ile Asp Lys Phe Gly 
                485                 490                 495     


Asp Ile Gln Leu Phe Glu Ala Leu Ala Ala Glu Glu Ala Leu Cys Val 
            500                 505                 510         


Trp Lys Pro Asp Gly Asn Pro Thr Ala Gln Pro Leu Lys Asp Phe Val 
        515                 520                 525             


Ala Ala Thr Glu Ala Asp Ala Lys Lys Lys Arg Phe Lys Val Pro Ala 
    530                 535                 540                 


Tyr Arg His Pro Asp Pro Leu Arg His Pro Val Phe Thr Asp Phe Gly 
545                 550                 555                 560 


Asn Ser Arg Trp Gly Ile Glu Tyr Ser Ala His Arg Ala Pro Ala Lys 
                565                 570                 575     


Cys Asp Glu Leu Gly Gln Gln Val Asp Arg Leu Thr Gln Ala Val Ala 
            580                 585                 590         


Glu Ala Gln Arg Asn Leu Asp Gly Ala Thr Ala Ala Gln Arg Ala Ser 
        595                 600                 605             


Arg Glu Ser Lys Leu Ala Glu Ala Gln Ser Lys Leu Val Ala Ala Gln 
    610                 615                 620                 


Thr Glu Phe Ala Ala Ile Asn Asp Pro His Arg Val Glu Leu Lys Leu 
625                 630                 635                 640 


Trp Asn Gly Gln Ala Val Ala Ala Ile Pro Met Arg Trp Ser Ser Lys 
                645                 650                 655     


Arg Leu Ile Ala Asp Leu Ser Leu Arg Arg Ala Thr Gln Pro Ser Ser 
            660                 665                 670         


Asp Gln Arg Ile Gly Val Thr Arg Ala Asp Arg Leu Gly Arg Ala Ala 
        675                 680                 685             


Gly Asn Ala Asp Asp Gly Arg Pro Val Thr Ile Thr Gly Leu Phe Gln 
    690                 695                 700                 


Gln Asp His Trp Asn Gly Arg Leu Gln Ala Pro Arg Ala Gln Leu Asp 
705                 710                 715                 720 


Ala Ile Ala Lys His Val Asp Lys His Gly Trp Asp Ala Lys Ala Arg 
                725                 730                 735     


Arg Gln Ile Ala Arg Ile Arg Trp Val Val Ser Phe Ser Ala Glu Leu 
            740                 745                 750         


Ser Gln Gln Gly Pro Trp Phe Glu Phe Cys His Arg Phe Gly Glu Asp 
        755                 760                 765             


Ala Pro Ala Arg Pro Phe Val Ser Arg His Gly Glu Tyr Ala Val Lys 
    770                 775                 780                 


His Arg Asp Asn Asp Gln Arg Lys Gly His Ala Lys Leu Ile Leu Ser 
785                 790                 795                 800 


Arg Leu Pro Gly Leu Arg Val Leu Ala Val Asp Leu Gly His Arg Tyr 
                805                 810                 815     


Ala Ala Ala Cys Ala Val Trp Glu Ala Ile Ser Ser Asp Gln Met Arg 
            820                 825                 830         


Gln Ala Cys Ala Ala Ala Asn Ala Pro Ala Pro His Pro Leu Ala Met 
        835                 840                 845             


Tyr Ile His Leu Lys Ser Thr Thr Ala Lys Gly Lys Pro Thr Thr Thr 
    850                 855                 860                 


Ile Tyr Arg Arg Ile Gly Pro Asp Lys Leu Pro Asp Gly Thr Pro His 
865                 870                 875                 880 


Pro Ala Pro Trp Ala Arg Leu Asp Arg Gln Phe Leu Ile Lys Leu Pro 
                885                 890                 895     


Gly Glu Asp Arg Pro Ala Arg Ala Ala Ser Pro Asp Glu Ile Lys Ala 
            900                 905                 910         


Val Glu Asp Phe Glu Asp Ser Val Gly Arg Val Arg Thr Ala Val Asp 
        915                 920                 925             


Pro Pro Arg Lys Arg Gly Val Asp Leu Leu Met His Asp Ala Val Arg 
    930                 935                 940                 


Thr Ala Arg Leu Ala Leu Ala Arg His Gly Arg Arg Ala Arg Ile Ala 
945                 950                 955                 960 


Phe Gln Leu Ile Ser Gln Val Arg Ile Leu Pro Gly Gly Arg Pro Gln 
                965                 970                 975     


Thr Leu Asp Asp Ala Gly Arg Arg Asp Leu Leu Asn Asp Thr Leu Ala 
            980                 985                 990         


Asp Trp Tyr Ala Leu Ala Thr Asp  Ser Arg Trp Thr Asp  Ala Ala Ala 
        995                 1000                 1005             


Arg Gln  Leu Trp Asn Glu Arg  Leu Ala Ala Leu Asn  Gly Gly Phe 
    1010                 1015                 1020             


Thr Ile  Asp Pro Pro Ala Asp  Ala Ser Gln Pro Glu  Ala Glu Arg 
    1025                 1030                 1035             


Thr Arg  Ala Gln Arg Arg Gln  Ala Glu Gln Glu Leu  Arg His Arg 
    1040                 1045                 1050             


Leu Ala  Ser Leu Val Glu Ala  Leu Phe Cys Asn Pro  Thr Leu Cys 
    1055                 1060                 1065             


Gln Gln  Leu His Gln Ala Trp  Thr Asp Arg Trp Asn  Ala Asp Asp 
    1070                 1075                 1080             


Gln Gln  Trp Arg Ser Arg Leu  Lys Trp Leu Ser Arg  Trp Leu Leu 
    1085                 1090                 1095             


Pro Arg  Gly Gly Ser Arg Arg  Asp Gly Ser Arg Arg  His Val Gly 
    1100                 1105                 1110             


Gly Leu  Ser Leu Thr Arg Ile  Ser Thr Leu Ile Asp  Phe Arg Arg 
    1115                 1120                 1125             


Lys Val  Gln Val Gly Tyr Phe  Thr Arg Leu Arg Pro  Asp Gly Ser 
    1130                 1135                 1140             


Arg Ala  Glu Ile Gly Pro Gln  Phe Gly Gln Ser Thr  Leu Asp Ala 
    1145                 1150                 1155             


Ile Gln  Arg Leu Lys Asp Gln  Arg Ile Lys Gln Leu  Thr Ser Arg 
    1160                 1165                 1170             


Ile Val  Glu Ala Ala Leu Gly  Ile Gly Val Glu Gln  Asp Arg Ile 
    1175                 1180                 1185             


Trp Asp  Ala Ala Lys Arg Lys  Trp Arg Thr Val Lys  Arg Pro Arg 
    1190                 1195                 1200             


Glu Pro  Arg Tyr His Val Asp  Asp Gln Gly Val Gln  Gln Arg Asp 
    1205                 1210                 1215             


Pro Arg  Phe Gln Ala Cys His  Ala Val Val Ile Glu  Asp Leu Ser 
    1220                 1225                 1230             


His Tyr  Arg Pro Glu Glu Thr  Arg Thr Arg Arg Glu  Asn Arg Ala 
    1235                 1240                 1245             


Thr Met  Asp Trp Lys Ser Ala  Glu Thr Arg Lys Arg  Leu Ala Asp 
    1250                 1255                 1260             


His Cys  Gln Leu Tyr Gly Leu  His Leu Arg Asp Val  Asn Pro Gln 
    1265                 1270                 1275             


Tyr Thr  Ser Arg Gln Asp Ser  Arg Thr Gly Ala Pro  Gly Cys Arg 
    1280                 1285                 1290             


Cys Val  Asp Val Ser Val Ala  Asp Phe Leu Thr Lys  Pro Ala Trp 
    1295                 1300                 1305             


Arg Lys  Gln Val Ala Gln Ala  Arg Gly Lys Val Ala  Ser Asn Arg 
    1310                 1315                 1320             


Gly Asp  Ala Arg Asp Arg Leu  Leu Val Glu Leu Asp  His Gln Leu 
    1325                 1330                 1335             


Thr Thr  Ala Asn Ser Leu Arg  Gly Glu Met Asp Ser  Leu Arg Ile 
    1340                 1345                 1350             


Pro Val  Asn Gly Gly Glu Val  Phe Val Ser Ala Asp  Pro Arg Ser 
    1355                 1360                 1365             


Pro Leu  Ala Ala Gly Ile Gln  Ala Asp Leu Asn Ala  Ala Ala Asn 
    1370                 1375                 1380             


Ile Gly  Leu Arg Ala Leu Met  Asp Pro Asp Phe Leu  Gly Thr Trp 
    1385                 1390                 1395             


Trp Tyr  Val Pro Cys Asp Pro  Ser Thr Lys Lys Pro  His Ile Glu 
    1400                 1405                 1410             


Lys Val  Lys Gly Ser Ile Leu  Ala Thr Val Gly Ala  Leu Gln Ala 
    1415                 1420                 1425             


Thr Ser  Glu Glu Ala Ala Ala  Pro Pro Arg Arg Gly  Arg Gly Gly 
    1430                 1435                 1440             


Thr Arg  Ser Ala Ala Pro Arg  Glu Val Ile Asn Leu  Trp Arg Asp 
    1445                 1450                 1455             


Pro Ser  Ala Val Arg Ile Gln  Asp Ala Thr Ala Gly  Glu Val Trp 
    1460                 1465                 1470             


Asp Val  Thr Pro Val Tyr Trp  Ser Ile Val Lys Asp  Arg Val Val 
    1475                 1480                 1485             


Asp Val  Leu Arg Gln Arg Asn  Thr Lys Ser Gly Asp  
    1490                 1495                 1500 


<210>  14
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CR RNA FOR ENGINEERED NUCLEASE

<400>  14
ggcggcgaat tcgccggcat ctcgatcgac cgacac                                 36


<210>  15
<211>  73
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRACR RNA FOR ENGINEERED NUCLEASE

<400>  15
cggagggaac tccgtgaacg tgtcttcccc ttcgatgggc ttggcacacg gggtcaatcg       60

agttgccgtt gaa                                                          73


<210>  16
<211>  1479
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ENGINEERED NUCLEASE

<400>  16

Met Ser Gln Gln Val Lys Pro Pro Val Thr Gln Arg Ala Tyr Thr Leu 
1               5                   10                  15      


Arg Leu Arg Gly Ile Asp Pro Ser Asp Thr Ser Trp Arg Lys Ala Leu 
            20                  25                  30          


Trp Gln Thr His Glu Gly Val Asn Lys Gly Ala Lys Ala Phe Gly Asp 
        35                  40                  45              


Trp Leu Leu Thr Leu Arg Gly Gly Leu Asp His Thr Leu Ala Asp Thr 
    50                  55                  60                  


Lys Val Lys Gly Gly Lys Gly Lys Pro Asp Arg Asp Pro Thr Asp Glu 
65                  70                  75                  80  


Glu Arg Lys Ala Arg Arg Ile Leu Leu Ala Leu Ser Trp Leu Ser Val 
                85                  90                  95      


Glu Ser Lys Leu Gly Ala Pro Val Gly Phe Ile Ile Ala Ser Gly Thr 
            100                 105                 110         


Glu Ala Ala Glu Asp Arg Asn Arg Lys Val Val Ala Ala Leu Glu Glu 
        115                 120                 125             


Ile Leu Lys Ser Arg Asn Val Ala Thr Asn Glu Ile Asp Gln Trp Lys 
    130                 135                 140                 


Asn Asp Cys Ser Ala Ser Leu Ser Ala Ala Ile Arg Asp Asp Ala Val 
145                 150                 155                 160 


Trp Val Asn Arg Ser Lys Ala Phe Asp Glu Ala Val Lys Ser Ile Gly 
                165                 170                 175     


Ser Ser Leu Thr Arg Glu Glu Ala Trp Asp Met Leu Glu Arg Phe Phe 
            180                 185                 190         


Gly Ser Arg Asp Ala Tyr Leu Ala Pro Ala Lys Gly Ser Glu Asp Glu 
        195                 200                 205             


Ser Ser Glu Thr Glu Gln Glu Asp Lys Ala Lys Asp Leu Val Gln Lys 
    210                 215                 220                 


Ala Gly Gln Trp Leu Ser Ser Arg Phe Gly Thr Gly Lys Gly Ala Asp 
225                 230                 235                 240 


Phe Cys Arg Met Ala Asn Val Tyr Gly Lys Ile Ala Ala Trp Ala Asp 
                245                 250                 255     


Asn Ala Gln Ala Asp Thr Thr Gly Asn Asp Ala Ile Asn Asn Leu Ala 
            260                 265                 270         


Ala Ala Leu Asn Glu Tyr Ser Pro Glu Pro Asn Asp Leu Lys Gly Val 
        275                 280                 285             


Leu Gly Leu Ile Ser Gly Pro Gly Tyr Lys Ser Ala Thr Arg Asn Leu 
    290                 295                 300                 


Leu Asn Gln Leu Ala Ala Lys Ala Thr Val Thr Gln Gln Asp Phe Val 
305                 310                 315                 320 


Ser Leu Lys Asp Lys Ala Ser Asn Asp Ala Gln Lys Cys Lys Gln Asn 
                325                 330                 335     


Thr Gly Ser Lys Gly Pro Arg Pro Tyr Ser Asp Ala Ile Leu Lys Asn 
            340                 345                 350         


Val Glu Ser Val Cys Gly Phe Thr Tyr Leu Gln Asp Gly Gly Pro Ala 
        355                 360                 365             


Arg His Ser Glu Phe Ala Val Ile Leu Asp His Ala Ala Arg Arg Val 
    370                 375                 380                 


Ser Leu Ala His Thr Trp Ile Lys Arg Ala Glu Ala Glu Arg Arg Lys 
385                 390                 395                 400 


Phe Glu Glu Asp Ala Lys Lys Ile Asp Gln Val Pro Lys Ala Ala Lys 
                405                 410                 415     


Asp Trp Leu Asp Ser Phe Cys Leu Glu Arg Ser Gly Ala Ser Gly Ala 
            420                 425                 430         


Leu Glu Pro Tyr Arg Ile Arg Arg Arg Ala Val Asp Gly Trp Lys Glu 
        435                 440                 445             


Val Val Ala Ala Trp Ser Lys Ala Asp Cys Lys Thr Ala Glu Asp Arg 
    450                 455                 460                 


Ile Ala Ala Ala Arg Ala Leu Gln Asp Asp Pro Glu Ile Asp Lys Phe 
465                 470                 475                 480 


Gly Asp Ile Gln Leu Phe Glu Ala Leu Ala Glu Asp Asp Ala Val Cys 
                485                 490                 495     


Val Trp His Lys Asp Gly Asp Ala Ala Lys Pro Pro Asp Pro Gln Pro 
            500                 505                 510         


Leu Ile Asp Tyr Ala Leu Ala Ala Glu Ala Glu Phe Lys Lys Arg His 
        515                 520                 525             


Phe Lys Val Pro Ala Tyr Arg His Pro Asp Ala Leu Leu His Pro Val 
    530                 535                 540                 


Phe Cys Asp Phe Gly Asn Ser Arg Trp Asp Ile Cys Phe Asp Val His 
545                 550                 555                 560 


Lys Asn Val Gln Thr Pro Phe Pro Arg Ala Leu Ser Leu Thr Leu Trp 
                565                 570                 575     


Thr Gly Ser Gly Met Val Ser Val Pro Leu Cys Trp Gln Ser Lys Arg 
            580                 585                 590         


Leu Ala Arg Asp Leu Ala Leu Gly Gln Asn Ala Gln Asn Asp Gly Ser 
        595                 600                 605             


Ser Glu Val Thr Arg Ala Asp Arg Leu Gly Arg Ala Ala Ser Asn Val 
    610                 615                 620                 


Thr Lys Ser Asp Glu Val Lys Ile Ser Gly Leu Phe Glu Gln Glu Asp 
625                 630                 635                 640 


Trp Asn Gly Arg Leu Gln Ala Pro Arg Gln Gln Leu Glu Ala Ile Ala 
                645                 650                 655     


Ala Val Arg Asp Asn Leu Ser Leu Ser Asp Gln Glu Arg Glu Arg Arg 
            660                 665                 670         


Met Ser Gly Met Ile Asp Arg Ile Arg Trp Leu Val Thr Phe Ser Ala 
        675                 680                 685             


Lys Leu Gln Pro Gln Gly Pro Trp Cys Glu Phe Ala Glu Lys Ile Gln 
    690                 695                 700                 


Ile Gly Ile Asn Pro Gln Tyr Trp Pro His Ala Asp Thr Asn Lys Asp 
705                 710                 715                 720 


Arg Lys Gly His Ala Arg Leu Ile Leu Ser Arg Leu Pro Gly Leu Arg 
                725                 730                 735     


Val Leu Ser Val Asp Leu Gly His Arg Tyr Ala Ala Ala Cys Ala Val 
            740                 745                 750         


Trp Glu Ala Val Asn Ala Glu Gln Ile Asn Val Ala Cys Arg Ala Ala 
        755                 760                 765             


Gly His Arg Glu Pro Lys Ala Ser Asp Leu Tyr Leu His Leu Lys Arg 
    770                 775                 780                 


Lys Thr Thr Lys Gln Lys Lys Gly Asp Gln Val Glu Phe Lys Glu Thr 
785                 790                 795                 800 


Thr Ile Tyr Arg Arg Ile Gly Ala Asp Thr Leu Pro Asp Gly Thr Pro 
                805                 810                 815     


His Pro Ala Pro Trp Ala Arg Leu Asp Arg Gln Phe Leu Ile Lys Leu 
            820                 825                 830         


Gln Gly Glu Glu Glu Gly Val Arg Ala Ala Ser Asn Glu Glu Ile Trp 
        835                 840                 845             


Ala Val His Arg Leu Glu Val Glu Leu Gly Arg Thr Ala Pro Leu Ile 
    850                 855                 860                 


Asp Arg Leu Val Lys Ala Gly Trp Gly Gln Ser Gly Lys Gln Lys Thr 
865                 870                 875                 880 


Arg Leu Asp Ala Leu Arg Asn Leu Gly Trp Ala Pro Ala Asn Glu Val 
                885                 890                 895     


Gln Gly Ser Asp Glu Met Asp Glu Gly Glu Val His Lys Pro Ser Leu 
            900                 905                 910         


Ser Val Asp Glu Leu Met Ser Ser Ala Val Arg Thr Met Arg Leu Ala 
        915                 920                 925             


Leu Lys Arg His Gly Asp Arg Ala Arg Ile Ala Phe Ala Met Thr Ala 
    930                 935                 940                 


Ala Tyr Lys Pro Met Pro Gly Asp Arg Lys Tyr Tyr Phe Thr Glu Ala 
945                 950                 955                 960 


Lys Asp Ala Ser Ala Asn Glu Asp Ala Thr Ala Arg Asn Gly Lys His 
                965                 970                 975     


Ile Glu Phe Ile Gln Asp Ala Leu Leu Leu Trp Tyr Gly Leu Thr Ser 
            980                 985                 990         


Ser Arg Gly Trp Arg Asp Asp Ala  Ala Arg Gln Leu Trp  Asp Asp His 
        995                 1000                 1005             


Ile Ala  Lys Leu Ser Val Tyr  Lys Ala Pro Glu Glu  Ile Gly Glu 
    1010                 1015                 1020             


Asp Ala  Ser Gly Val Glu Arg  Lys Thr Lys Gln Lys  Glu Asn Arg 
    1025                 1030                 1035             


Glu Lys  Leu His Asp Val Ala  Lys Ala Leu Ala Gln  Asp Val Asn 
    1040                 1045                 1050             


Leu Arg  Lys Val Leu His Asn  Ala Trp Lys Lys Arg  Trp Glu Lys 
    1055                 1060                 1065             


Asp Asp  Glu Arg Trp Lys Lys  Gln Leu Arg Trp Phe  Lys Asp Trp 
    1070                 1075                 1080             


Val Phe  Pro Arg Gly Lys His  Ala Ser Asp Pro Ala  Ile Arg Lys 
    1085                 1090                 1095             


Val Gly  Gly Leu Ser Leu Pro  Arg Leu Ala Thr Leu  Ile Glu Phe 
    1100                 1105                 1110             


Arg Arg  Lys Val Gln Val Gly  Phe Phe Thr Arg Leu  Gln Pro Asp 
    1115                 1120                 1125             


Gly Thr  Arg Ala Glu Thr Lys  Glu Gln Phe Gly Gln  Ser Ala Leu 
    1130                 1135                 1140             


Asp Thr  Leu Glu His Leu Arg  Glu Gln Arg Val Lys  Gln Leu Ala 
    1145                 1150                 1155             


Ser Arg  Ile Val Glu Ala Ala  Leu Gly Ile Gly Arg  Val Arg Arg 
    1160                 1165                 1170             


Pro Leu  Gly Gly Lys Asp Pro  Lys Arg Pro Asp Val  Arg Val Asp 
    1175                 1180                 1185             


Glu Pro  Cys His Ala Ile Val  Ile Glu Asp Leu Thr  His Tyr Arg 
    1190                 1195                 1200             


Pro Glu  Glu Thr Arg Thr Arg  Arg Glu Asn Arg Gln  Leu Met Thr 
    1205                 1210                 1215             


Trp Ser  Ser Ser Lys Val Lys  Asp Tyr Leu Ser Glu  Ala Cys Gln 
    1220                 1225                 1230             


Leu His  Gly Leu His Leu Arg  Glu Val Ser Ala Ser  Tyr Thr Ser 
    1235                 1240                 1245             


Arg Gln  Asp Ser Arg Thr Gly  Ser Pro Gly Ile Arg  Cys Gln Asp 
    1250                 1255                 1260             


Val Pro  Val Lys Glu Phe Met  Arg Ser Pro Phe Trp  Arg Lys Gln 
    1265                 1270                 1275             


Val Ala  Gln Ala Glu Lys Lys  Gly Asp Ala His Glu  Arg Phe Leu 
    1280                 1285                 1290             


Cys Glu  Leu Asn Ala Met Trp  Lys Asp Lys Thr Val  Ala Asp Trp 
    1295                 1300                 1305             


Glu Lys  Ala Gly Ala Val Arg  Val Pro Leu Lys Gly  Gly Glu Val 
    1310                 1315                 1320             


Phe Val  Ser Ala Asp Arg Ile  Ser Pro Ser Ala Lys  Gly Leu Gln 
    1325                 1330                 1335             


Ala Asp  Leu Asn Ala Ala Ala  Asn Ile Gly Leu Arg  Ala Leu Thr 
    1340                 1345                 1350             


Asp Pro  Asp Trp Pro Gly Lys  Trp Trp Tyr Val Pro  Cys Glu Pro 
    1355                 1360                 1365             


Ala Ser  Phe Arg Pro Ala Lys  Asp Lys Val Asp Gly  Ser Ala Val 
    1370                 1375                 1380             


Val Asn  Pro Gly Gln Pro Leu  Arg Gln Ser Ala Gln  Ala Gln Ser 
    1385                 1390                 1395             


Gly Asp  Ala Ala Lys Asp Lys  Lys Lys Arg Gly Asn  Lys Gly Ala 
    1400                 1405                 1410             


Gly Gln  Ser Lys Glu Val Val  Asn Leu Trp Arg Asp  Ile Ser Ser 
    1415                 1420                 1425             


Ser Pro  Leu Glu Cys Ile Glu  Cys Gly Glu Trp Lys  Glu Tyr Ala 
    1430                 1435                 1440             


Ala Tyr  Gln Asn Glu Val Gln  Tyr Arg Val Ile Arg  Ile Leu Glu 
    1445                 1450                 1455             


Glu Gln  Ile Lys Gly Arg Asp  Arg Gln Pro His Glu  Gly Ser Arg 
    1460                 1465                 1470             


Glu Asp  Asp Ile Pro Phe 
    1475                 


<210>  17
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CR RNA

<400>  17
gttgccgacg tcagcaccaa cctgatcgac ggacac                                 36


<210>  18
<211>  93
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRACR RNA FOR ENGINEERED NUCLEASE

<400>  18
ttgcctctac aggaggcgag aatgccacgg cacgtgtctt ccccttcaat gggcttggca       60

ccgtggagtc gatcagtttt gtgccggcga agg                                    93


<210>  19
<211>  1481
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ENGINEERED NUCLEASE

<400>  19

Met Ala Arg Arg Ser Ala Ser Thr Ser Lys Pro Pro Gly Pro Val Ala 
1               5                   10                  15      


Pro Thr Thr Gln Arg Ala Tyr Thr Leu Arg Leu Arg Arg Ala Pro Gly 
            20                  25                  30          


Lys Cys Pro His Cys Glu Gln Asp Ala Cys Asp Cys Trp Arg Glu Ala 
        35                  40                  45              


Leu Trp Ala Thr His Ala Ala Phe Asn Arg Gly Ala Lys Ala Phe Gly 
    50                  55                  60                  


Asp Trp Leu Leu Thr Leu Arg Gly Gly Leu Ser His Glu Leu Ala Glu 
65                  70                  75                  80  


Gln Pro Ser Pro Pro Lys Asn Lys Glu Pro Thr Cys Glu Glu Ser Asp 
                85                  90                  95      


Ala Ile Arg Lys Asn Arg Arg Ile Leu Leu Ala Leu Ser Trp Leu Ser 
            100                 105                 110         


Val Glu Asp Asp Cys Ser Ala Pro Thr Gly Thr Phe Arg Val Ala Ser 
        115                 120                 125             


Gly Lys Asp Ser Glu Ala Glu Arg Lys Asn Lys Val Leu Thr Ala Phe 
    130                 135                 140                 


Arg Ser Ile Leu Thr Ala Arg Arg Met Arg Ser Gln Asp Val Glu Ser 
145                 150                 155                 160 


Trp Ile Ala Asp Cys Ala Ala Ser Leu Ser Ala Lys Ile Arg Glu Asp 
                165                 170                 175     


Ala Val Trp Ile Asn Arg Ser Ala Cys Phe Asp Gln Arg Ala Leu Asp 
            180                 185                 190         


Leu Lys Val Leu Ser Arg Glu Tyr Ala Lys Ala Ala Val Met Ser Phe 
        195                 200                 205             


Phe Gly Pro Leu Asp Glu Tyr Phe Lys Leu Pro Asp Glu Ala Asp Asp 
    210                 215                 220                 


Thr Lys Pro Ala Val Gly Gly Asp Gly Pro Asp Phe Arg Thr Leu Ala 
225                 230                 235                 240 


Arg Gln Trp Val Ser Thr Asn Phe Gly Thr Gly Lys Lys Ser Asp Ser 
                245                 250                 255     


Glu Ala Ile Ala Gln Asn Leu Arg Lys Leu Ala Asp Ala Asn Leu Ala 
            260                 265                 270         


Pro Phe Ser Gly Lys Pro Lys Ala Ala Leu Ile Ala His Leu Ser Val 
        275                 280                 285             


Glu Leu Asp Gly Ser Thr Ala Asp Ile Asp Gly Leu Cys Arg Ala Ile 
    290                 295                 300                 


Gly Trp Asn Thr Gly Arg Pro Ser Lys Gly Arg Val Ala Ile Glu Arg 
305                 310                 315                 320 


Leu Pro Asp Pro Pro Thr Glu Thr Ser Ile Gln Thr Met Gln Gln Lys 
                325                 330                 335     


Phe Arg Glu Glu Ala Glu Ala Lys Ala Ser Ser Lys Gly Leu Arg Gln 
            340                 345                 350         


Val Pro Glu Trp Met Pro Ala Phe Gln Lys Ser Ile Glu Arg Asp Cys 
        355                 360                 365             


Gly Met Pro Phe Lys Leu Gly Glu Gly Arg Asp His Ile Gly Glu Phe 
    370                 375                 380                 


Ser Val Met Leu Asp His Ala Ala Arg Arg Val Ser Ile Gly His Ser 
385                 390                 395                 400 


Trp Ile Lys Arg Ala Glu Ala Glu Arg Arg Arg Phe Glu Ala Asp Ala 
                405                 410                 415     


Gln Arg Leu Asn His Ile Pro Ala Ala Ala Lys Asp Trp Leu Asp Gln 
            420                 425                 430         


Phe Val Gln Phe Arg Ser Gly Ser Ser Gly Ala Ala Ala Ala Gly Gly 
        435                 440                 445             


Glu Tyr Arg Ile Arg Arg Arg Ala Ile Glu Gly Trp Asp Glu Ile Ile 
    450                 455                 460                 


Lys Arg Trp Lys Arg Ala Ala Cys Lys Ser Pro Glu Asp Arg Val Ala 
465                 470                 475                 480 


Ala Ala Arg Glu Val Gln Ala Asp Pro Glu Ile Glu Lys Phe Gly Asp 
                485                 490                 495     


Ile Gln Leu Phe Glu Ala Leu Ala Ala Asp Asp Ala Glu Cys Val Trp 
            500                 505                 510         


Arg Gly Asp Gly Asn Gly Thr Pro Asp Pro Leu Lys Asp Tyr Val Ala 
        515                 520                 525             


Ala Thr Asp Ala Leu Asp Lys Met Arg Arg Phe Lys Val Pro Ala Tyr 
    530                 535                 540                 


Arg His Pro Asp Pro Leu Ala His Pro Val Phe Gly Asp Phe Gly Asn 
545                 550                 555                 560 


Ser Arg Gly Asp Ile Arg Phe Ala Val His Glu Ala Ala Lys Ala Thr 
                565                 570                 575     


Arg Gly Thr Lys Arg Ile Ala Lys Asp Gln Lys Glu Trp Ile Arg Glu 
            580                 585                 590         


Arg His Gly Leu Arg Met Gly Leu Trp Asp Gly Gln Ser Val Arg Thr 
        595                 600                 605             


Ala Asp Leu Arg Trp Ser Ser Lys Arg Leu Val Asp Asp Leu Ala Leu 
    610                 615                 620                 


Arg Asn His Val Thr Thr Arg Arg Thr Gly Pro Val Ser Arg Ala Asp 
625                 630                 635                 640 


Arg Leu Gly Arg Ala Ala Ala Gly Leu Gly Ala Asp Glu Ala Ala Cys 
                645                 650                 655     


Val Ala Gly Leu Phe Glu Leu Pro Asp Trp Asn Gly Arg Leu Gln Ala 
            660                 665                 670         


Pro Arg Ala Gln Leu Asp Ala Ile Ala Ala Cys Val Ala Ala Asn Gly 
        675                 680                 685             


Gly Lys Trp Asp Asp Lys Ala Arg Lys Leu Arg Asp Arg Ile Glu Trp 
    690                 695                 700                 


Leu Val Ser Phe Ser Ala Lys Leu Glu Cys Cys Gly Pro Phe Met Glu 
705                 710                 715                 720 


Tyr Ala Ser Gln Asn Gly Ile Gln Pro Asn Gly Lys Gly Glu Tyr Tyr 
                725                 730                 735     


Pro His Ala Glu Arg Asn Lys Gly Arg Thr Gly His Ala Lys Leu Ile 
            740                 745                 750         


Leu Ser Arg Leu Pro Gly Leu Arg Val Leu Ala Val Asp Leu Gly His 
        755                 760                 765             


Arg Phe Ala Ala Ala Cys Ala Val Trp Glu Ala Leu Ser Lys Ile Ala 
    770                 775                 780                 


Phe Asp Ala Glu Thr Lys Gly Arg Glu Val Val Ser Gly Gly Arg Ala 
785                 790                 795                 800 


Ala Asp Asp Leu Tyr Cys His Thr Arg His Leu Asp Cys Ala Gly Lys 
                805                 810                 815     


Ala Arg Thr Thr Ile Tyr Arg Arg Ile Gly Pro Asp Lys Leu Pro Asp 
            820                 825                 830         


Gly Ser Asp His Pro Ala Pro Trp Ala Arg Leu Asp Arg Gln Phe Leu 
        835                 840                 845             


Ile Lys Leu Gln Gly Glu Glu Arg Pro Ala Arg Ala Ala Gly Pro Ala 
    850                 855                 860                 


Glu Thr Ala Ala Val Gln Gln Ile Glu Thr Asp Leu Gly Arg Ala Arg 
865                 870                 875                 880 


Gly Gln Glu Asp Leu Pro Pro Arg Pro Val Asp Ser Leu Met Arg Glu 
                885                 890                 895     


Ala Val Arg Thr Ile Arg Ile Ala Leu Arg Arg His Gly Asp Ala Ala 
            900                 905                 910         


Arg Ile Ala Tyr Ala Phe Lys Pro Gly Ala Lys Arg Leu Lys Pro Gly 
        915                 920                 925             


Gly Gly Ala Gln Asp His Thr Pro Glu Thr His Ala Asp Ala Ile Leu 
    930                 935                 940                 


Glu Ala Leu Leu Arg Trp His Glu Leu Ala Thr Gly Ala Arg Trp Arg 
945                 950                 955                 960 


Asp Pro Trp Ala Glu Thr Gln Trp Lys Asp Trp Val Gln Pro His Ile 
                965                 970                 975     


Ser Ala Thr Leu Pro Glu Leu Ala Asn Asp Ala Asp Arg Trp Glu Arg 
            980                 985                 990         


Lys Arg His Arg Ala Ala Leu Glu  Gln Val Leu Arg Pro  Val Ala Gln 
        995                 1000                 1005             


Met Leu  Ile Gln Arg Pro Thr  Asp Ala Leu His Gln  Val Trp Ser 
    1010                 1015                 1020             


Lys His  Trp Ala Asp Glu Asp  Leu Lys Trp Pro Ser  Arg Leu Arg 
    1025                 1030                 1035             


Trp Leu  Arg Asn Trp Leu Leu  Pro Arg Gly Pro Arg  Ala Arg Ser 
    1040                 1045                 1050             


Gly Ala  Ala Arg Asn Val Gly  Gly Leu Ser Leu Leu  Arg Ile Ala 
    1055                 1060                 1065             


Thr Leu  Arg Glu Leu Tyr Gln  Thr Gln Lys Ala Tyr  Ala Met Arg 
    1070                 1075                 1080             


Pro Glu  Pro Asp Asp Pro Arg  Lys Arg Ile Ala Gly  Arg Asn Asp 
    1085                 1090                 1095             


Asp Arg  Tyr Asp Glu Leu Gly  Arg Ser Val Leu Gln  Val Ile Glu 
    1100                 1105                 1110             


Arg Leu  Arg Glu Gln Arg Val  Lys Gln Leu Ala Ser  Arg Ile Val 
    1115                 1120                 1125             


Glu Ala  Ala Leu Gly Val Gly  Arg Ala Lys Pro Thr  Arg Gly Arg 
    1130                 1135                 1140             


Gln Arg  Pro Gln Ser Arg Val  Asp Val Pro Cys His  Ala Val Ile 
    1145                 1150                 1155             


Ile Glu  Ser Leu Arg Asn Tyr  Arg Pro Asp Glu Leu  Gln Thr Arg 
    1160                 1165                 1170             


Arg Glu  Asn Arg Ala Ile Met  Asn Trp Ser Ala Gly  Lys Val Arg 
    1175                 1180                 1185             


Lys Tyr  Leu Glu Glu Ala Cys  Gln Leu His Gly Leu  His Leu Arg 
    1190                 1195                 1200             


Glu Val  Met Pro Asn Tyr Thr  Ser Arg Glu Asp Ser  Arg Thr Gly 
    1205                 1210                 1215             


Leu Pro  Gly Val Arg Cys Val  Asp Val Pro Val Asp  Pro Lys Leu 
    1220                 1225                 1230             


Gly Lys  Pro Lys Ala Tyr Trp  Trp Asn Ser Val Leu  Ser Thr Ala 
    1235                 1240                 1245             


Arg Lys  Lys Ser Ile Gly Asp  Ala Ala Ser His Asp  Lys Gln Gly 
    1250                 1255                 1260             


Asp Ala  Thr Ser Arg Phe Ile  Val Glu Leu Ala Gly  Cys Leu Asp 
    1265                 1270                 1275             


Arg Leu  Lys Ala Asp Gly Lys  Pro Leu Pro Lys Thr  Val Arg Val 
    1280                 1285                 1290             


Pro Arg  Ile Gly Gly Asp Leu  Phe Val Ala Ala Pro  Pro Thr Ser 
    1295                 1300                 1305             


Cys Thr  Ala Pro Ala His Gln  Pro His Pro Ala Cys  Asp Gly Ala 
    1310                 1315                 1320             


Arg Ala  Leu Gln Ala Asp Leu  Asn Ala Ala Ala Asn  Ile Gly Leu 
    1325                 1330                 1335             


Arg Ala  Leu Leu Asp Pro Asp  Phe Pro Ala Lys Trp  Trp Tyr Val 
    1340                 1345                 1350             


Pro Cys  Ile Asp Asp Gln Arg  Gly Leu Ala Leu Pro  Arg Ala Asp 
    1355                 1360                 1365             


Lys Val  Leu Gly Ser Ala Cys  Phe Pro Gly Asp Pro  Ala Thr Phe 
    1370                 1375                 1380             


Gly Ser  Leu Leu Lys Thr Arg  Thr Ala Ala Gly Pro  Ala Val Asp 
    1385                 1390                 1395             


Gly Gln  Ala Ala Pro Asp Arg  Lys Pro Arg Thr Gly  Thr His Arg 
    1400                 1405                 1410             


Pro Gly  Ser Ala Lys Ser Arg  Ser Leu Gly Asp Gly  Lys Ala Thr 
    1415                 1420                 1425             


Thr Asn  Tyr Trp Ser Asp Arg  Ser Ala Arg Asp Leu  Arg Pro Ala 
    1430                 1435                 1440             


Asp Glu  Gly Gly His Trp Gln  Pro Thr Asn Val Tyr  Trp Asn Trp 
    1445                 1450                 1455             


Val Arg  Lys Arg Ala Leu Leu  Gly Leu Tyr Ser Phe  Asn Gly Leu 
    1460                 1465                 1470             


Ser Pro  Pro Ser Asp Asp Arg  Pro 
    1475                 1480     


<210>  20
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CR RNA FOR ENGINEERED NUCLEASE

<400>  20
gccgcgtcgg ccgacgcggc cttgatcgat ggacac                                 36


<210>  21
<211>  94
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ENGINEERED NUCLEASE

<400>  21
gtcgcctata gggcgataca actccgagca tgtgtcttcc ccttcaatgg gcttggcact       60

cggcatcgat caagctcgcg tcggctgtcg ggcc                                   94


<210>  22
<211>  111
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  22
gggaagggcc tatttcccag catgtgtctt cgcatttaat tgctttagca ctgggcatcg       60

ttctttttag tagtgtttaa gtagatacta ctgaaaagac cgatggacac a               111


<210>  23
<211>  98
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST GRNA

<400>  23
gggaagggcc tatttcccag catgtgtctt cgcatttaat tgctttagca ctgggcatcg       60

ttctttttag aaaactactg aaaagaccga tggacaca                               98


<210>  24
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  24
gggaagggcc tatttcccag catgtgtctt cgcatttaat tgctttagca ctgggcatcg       60

ttctaaaaag accgatggac aca                                               83


<210>  25
<211>  97
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  25
ggaagggcct atttcccagc atgtgtcttc gcatttaatt gctttagcac tgggcatcgt       60

tctttttaga aaactactga aaagaccgat ggacaca                                97


<210>  26
<211>  105
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  26
ggtccaaggg aagggcctat ttcccagcat gtgtcttcgc atttaattgc tttagcactg       60

ggcatcgttc tttttagaaa actactgaaa agaccgatgg acaca                      105


<210>  27
<211>  116
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  27
gccccgattt ccctttgaat gatctcggcc tcgttgccac tgaccgaatt cttccgcctt       60

tggaattcca agctctttga catcgcgagc ccgcgatggg aaagagattg tggcgg          116


<210>  28
<211>  114
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  28
gccccgattt ccctttgaat gatctcggcc tcgttgccac tgaccgaatt cttccgcctt       60

tggaattcca agctctttga catcgcgagc ccgcgatggg aaagagattg tggc            114


<210>  29
<211>  111
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  29
gatttccctt tgaatgatct cggcctcgtt gccactgacc gaattcttcc gcctttggaa       60

ttccaagctc tttgacatcg cgagcccgcg atgggaaaga gattgtggcg g               111


<210>  30
<211>  112
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNAV4

<400>  30
ggatttccct ttgaatgatc tcggcctcgt tgccactgac cgaattcttc cgcctttgga       60

attccaagct ctttgacatc gcgagcccgc gatgggaaag agattgtggc gg              112


<210>  31
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  31
gggaagggcc tatttcccag catgtgtctt cgcatttaat tgctttagca ctgggcatcg       60

ttctttttag tagtgtttaa gtagatacta ctaaaaagac cgatggacac                 110


<210>  32
<211>  96
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  32
gggaagggcc tatttcccag catgtgtctt cgcatttaat tgctttagca ctgggcatcg       60

ttctttttag taaaaactaa aaagaccgat ggacac                                 96


<210>  33
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  33
gggaagggcc tatttcccag catgtgtctt cgcatttaat tgctttagca ctgggcatcg       60

ttcttaaaaa agaccgatgg acac                                              84


<210>  34
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  34
gggccaaggg aagggcctat ttcccagcat gtgtcttcgc atttaattgc tttagcactg       60

ggcatcgttc tttttagtaa aaactaaaaa gaccgatgga cac                        103


<210>  35
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  35
gggccaaggg aagggcctat ttcccagcat gtgtcttcgc atttaattgc tttagcactg       60

ggcatcgttc ttaaaaaaga ccgatggaca c                                      91


<210>  36
<211>  134
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  36
atgtaaccct ataggggttg cgtgagtcgg ccatagtgcc tcggcaacag cgtaaaaaac       60

tgctgccagt ggtcgaagta agtcaacaaa acggaggtgg tgaagtcacc cccgttttgt      120

aggcctactg gcac                                                        134


<210>  37
<211>  111
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  37
ggatgtaacc ctataggggt tgcgtgagtc ggccatagtg cctcggcaac agcgtaaaaa       60

actgctgcca gtggtcgaag taagtcaaca aaaatgtagg cctactggca c               111


<210>  38
<211>  108
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  38
ggtaacccta taggggttgc gtgagtcggc catagtgcct cggcaacagc gtaaaaaact       60

gctgccagtg gtcgaagtaa gtcaacaaaa atgtaggcct actggcac                   108


<210>  39
<211>  112
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  39
gggatgtaac cctatagggg ttgcgtgagt cggccatagt gcctcggcaa cagcgtaaaa       60

aactgctgcc agtggtcgaa gtaagtcaac aaaaatgtag gcctactggc ac              112


<210>  40
<211>  109
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  40
gggtaaccct ataggggttg cgtgagtcgg ccatagtgcc tcggcaacag cgtaaaaaac       60

tgctgccagt ggtcgaagta agtcaacaaa aatgtaggcc tactggcac                  109


<210>  41
<211>  109
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  41
cggagggaac tccgtgaacg tgtcttcccc ttcgatgggc ttggcacacg gggtcaatcg       60

agttgccgtt gaaggcggcg aattcgccgg catctcgatc gaccgacac                  109


<210>  42
<211>  91
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  42
ggagggaact ccgtgaacgt gtcttcccct tcgatgggct tggcacacgg ggtcaatcga       60

gttgccaaaa ggcatctcga tcgaccgaca c                                      91


<210>  43
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  43
ggagggaact ccgtgaacgt gtcttcccct tcgatgggct tggcacacgg ggtcaatcga       60

gttatctcga tcgaccgaca c                                                 81


<210>  44
<211>  98
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  44
ggcaggcgga gggaactccg tgaacgtgtc ttccccttcg atgggcttgg cacacggggt       60

caatcgagtt gccaaaaggc atctcgatcg accgacac                               98


<210>  45
<211>  88
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  45
ggcaggcgga gggaactccg tgaacgtgtc ttccccttcg atgggcttgg cacacggggt       60

caatcgagtt atctcgatcg accgacac                                          88


<210>  46
<211>  129
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  46
ttgcctctac aggaggcgag aatgccacgg cacgtgtctt ccccttcaat gggcttggca       60

ccgtggagtc gatcagtttt gtgccggcga agggttgccg acgtcagcac caacctgatc      120

gacggacac                                                              129


<210>  47
<211>  93
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  47
gcctctacag gaggcgagaa tgccacggca cgtgtcttcc ccttcaatgg gcttggcacc       60

gtggagtcga tcagaaaact gatcgacgga cac                                    93


<210>  48
<211>  95
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  48
gggcctctac aggaggcgag aatgccacgg cacgtgtctt ccccttcaat gggcttggca       60

ccgtggagtc gatcagaaaa ctgatcgacg gacac                                  95


<210>  49
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  49
gggacattgg cctctacagg aggcgagaat gccacggcac gtgtcttccc cttcaatggg       60

cttggcaccg tggagtcgat cagaaaactg atcgacggac ac                         102


<210>  50
<211>  133
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  50
gtcgcctata gggcgataca actccgagca tgtgtcttcc ccttcaatgg gcttggcact       60

cggcatcgat caagctcgcg tcggctgtcg ggccaaagcc gcgtcggccg acgcggcctt      120

gatcgatgga cac                                                         133


<210>  51
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  51
gtcgcctata gggcgataca actccgagca tgtgtcttcc ccttcaatgg gcttggcact       60

cggcatcgat caagctcgcg tcggctgtcg tcggccgacg cggccttgat cgatggacac      120


<210>  52
<211>  108
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  52
gtcgcctata gggcgataca actccgagca tgtgtcttcc ccttcaatgg gcttggcact       60

cggcatcgat caagctcgcg taaaaacgcg gccttgatcg atggacac                   108


<210>  53
<211>  121
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  53
ggtcgcctat agggcgatac aactccgagc atgtgtcttc cccttcaatg ggcttggcac       60

tcggcatcga tcaagctcgc gtcggctgtc gtcggccgac gcggccttga tcgatggaca      120

c                                                                      121


<210>  54
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TEST SGRNA

<400>  54
gggtcgccta tagggcgata caactccgag catgtgtctt ccccttcaat gggcttggca       60

ctcggcatcg atcaagctcg cgtaaaaacg cggccttgat cgatggacac                 110


<210>  55
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRIMER SEQUENCE

<400>  55
ttgggtaacg ccagggtttt                                                   20


<210>  56
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRIMER SEQUENCE

<400>  56
tgtgtggaat tgtgagcgga                                                   20


<210>  57
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRIMER SEQUENCE

<400>  57
aaacccctcc gtttagagag                                                   20


<210>  58
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRIMER SEQUENCE

<400>  58
aagctaatac gactcactat aggccagtc                                         29


<210>  59
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRIMER SEQUENCE

<400>  59
ccagtcagta atgttactgg                                                   20


<210>  60
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRIMER SEQUENCE

<400>  60
ccggtggtgc agatgaactt cag                                               23


<210>  61
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRIMER SEQUENCE

<400>  61
aagaagtcgt gctgcttcat gtg                                               23


<210>  62
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRIMER SEQUENCE

<400>  62
ccgtaggtgg catcgccctc gcc                                               23


<210>  63
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRIMER SEQUENCE

<400>  63
gggcatggcg gacttgaaga agt                                               23


<210>  64
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRIMER SEQUENCE

<400>  64
tggccgttta cgtcgccgtc cag                                               23


<210>  65
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRIMER SEQUENCE

<400>  65
aagaagatgg tgcgctcctg gac                                               23


<210>  66
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRIMER SEQUENCE

<400>  66
gccggtggtg cagatgaact tca                                               23


<210>  67
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRIMER SEQUENCE

<400>  67
atctgcacca ccggcaaact gcc                                               23


<210>  68
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRIMER SEQUENCE

<400>  68
agcgtgtccg gcgagggcga ggg                                               23


<210>  69
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRIMER SEQUENCE

<400>  69
gtgaccaccc tgacctacgg cgt                                               23


<210>  70
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRIMER SEQUENCE

<400>  70
agccgctacc ccgaccacat gaa                                               23


<210>  71
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRIMER SEQUENCE

<400>  71
ttcaagtccg ccatgcccga agg                                               23


<210>  72
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRIMER SEQUENCE

<400>  72
ttcaaggacg acggcaacta caa                                               23


<210>  73
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRIMER SEQUENCE

<400>  73
aaggacgacg gcaactacaa gac                                               23


