                         б

<110>  ҩƷ޹˾

<120>  PD-L1/4-1BBȻṹԴʽ˫쿹弰Ʊ

<130>  LZ2120047CN07

<160>  26

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  321
<212>  DNA
<213>  ˹(Artificial Sequence)

<220>
<223>  ɱ


<400>  1
gacatccaga tgacccagtc cccttcctcc ctgtccgcct ctgtgggcga cagggtgacc        60
atcacctgca aggcctccca ggatgtgcac accgccgtgg cttggattca gcagaagccc       120
ggcaagtccc ccaagctgct gatctactcc gcctccaacc ggtacaccgg cgtgcctagc       180
aggttcagcg gaagcggcag cggcaccgac ttcaccttca ccatctcctc cctgcagcct       240
gaggacatcg ccacctatta ctgccagcag cactacatca cccccctgac cttcggccag       300
ggcaccaagc tggagatcaa g                                                 321

<210>  2
<211>  107
<212>  PRT
<213>  ˹(Artificial Sequence)

<220>
<223>  ɱ


<400>  2
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Val His Thr Ala 
            20                  25                  30          
Val Ala Trp Ile Gln Gln Lys Pro Gly Lys Ser Pro Lys Leu Leu Ile 
        35                  40                  45              
Tyr Ser Ala Ser Asn Arg Tyr Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  
Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln His Tyr Ile Thr Pro Leu 
                85                  90                  95      
Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105         

<210>  3
<211>  321
<212>  DNA
<213>  ˹(Artificial Sequence)

<220>
<223>  㶨


<400>  3
cgtacggtgg ccgcccccag cgtcttcatt tttccaccct ctgacgaaca gctgaagtca        60
gggacagctt ccgtggtctg tctgctgaac aatttttacc ccagggaggc caaagtgcag       120
tggaaggtcg ataacgctct gcagagcgga aattctcagg agagtgtgac agaacaggac       180
tcaaaagatt ccacttatag cctgtctagt accctgacac tgtccaaggc agactacgaa       240
aagcataaag tgtatgcctg tgaggtcaca catcagggtc tgtcaagccc cgtcactaag       300
tccttcaacc ggggagaatg c                                                 321

<210>  4
<211>  107
<212>  PRT
<213>  ˹(Artificial Sequence)

<220>
<223>  㶨


<400>  4
Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      
Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          
Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              
Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  
Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  
Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      
Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         

<210>  5
<211>  351
<212>  DNA
<213>  ˹(Artificial Sequence)

<220>
<223>  ɱ


<400>  5
gaagtgcagc tggtgcagtc cggcgccgag gtgaagaagc ctggctcctc cgtgaaggtg        60
tcctgcaagg cctccggctt caacatcgag gacacctaca tccactgggt gaggcaggct       120
cccggacagg gactggagtg gatgggcagg atcgatcccg ccaacgccaa caccaagtac       180
gaccccaagt tccagggcag ggtgaccatc accgccgata ccagcaccaa caccgcctac       240
atggagctgt cctccctgag gtccgaggac acagccgtgt actactgtgg caggggactg       300
ggcgcctggt ttgcctactg gggacagggc accctggtga ccgtgtcctc c                351

<210>  6
<211>  117
<212>  PRT
<213>  ˹(Artificial Sequence)

<220>
<223>  ɱ


<400>  6
Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      
Ser Val Lys Val Ser Cys Lys Ala Ser Gly Phe Asn Ile Glu Asp Thr 
            20                  25                  30          
Tyr Ile His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              
Gly Arg Ile Asp Pro Ala Asn Ala Asn Thr Lys Tyr Asp Pro Lys Phe 
    50                  55                  60                  
Gln Gly Arg Val Thr Ile Thr Ala Asp Thr Ser Thr Asn Thr Ala Tyr 
65                  70                  75                  80  
Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Gly Arg Gly Leu Gly Ala Trp Phe Ala Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         
Val Thr Val Ser Ser 
        115         

<210>  7
<211>  990
<212>  DNA
<213>  ˹(Artificial Sequence)

<220>
<223>  㶨


<400>  7
gctagcacaa aaggaccttc cgtgtttcca ctggcaccct ctagtaagag tacttcagga        60
ggaaccgcag cactgggatg tctggtgaag gactacttcc cagagcccgt caccgtgtct       120
tggaacagtg gagcactgac ctccggggtc catacatttc ctgccgtgct gcagtcatcc       180
ggtctgtata gcctgagctc tgtggtcaca gtcccaagtt catccctggg cacccagaca       240
tacatctgca acgtgaatca caaaccttcc aatactaagg tcgacaagaa agtggaaccc       300
aagtcctgcg ataagaccca cacatgccct ccctgtcctg ctcccgaact gctgggagga       360
ccctccgtct tcctgttccc ccccaagccc aaagacacac tgatgatcag caggacccct       420
gaagtgacct gcgtggtcgt ggacgtgagc cacgaggacc ccgaggtcaa gtttaactgg       480
tacgtggacg gcgtggaggt ccacaacgcc aagaccaagc ccagggagga gcagtacaac       540
agcacctaca gggtcgtgtc cgtgctgacc gtgctccacc aagattggct caacggcaag       600
gagtataagt gcaaagtcag caacaaggcc ctccccgccc ccatcgagaa aaccatcagc       660
aaggccaagg gccaaccgcg ggaacctcaa gtgtataccc tccctcccag ccgggatgag       720
ctgaccaaga accaagtctc cctcttgtgc ctggtcaagg gattctaccc ttccgacatt       780
gccgtcgaat gggagagcaa tggccagccc gagaacaact acaagacaac cccccccgtc       840
ctgcgcagcg acggatcctt cttcctgtac tccaagctca ccgtggacaa gagccggtgg       900
caacagggca acgtgttctc ctgtagcgtg atgcacgaag ccctccacaa ccactatacc       960
cagaagagcc tgagcctcag ccccggcaaa                                        990

<210>  8
<211>  330
<212>  PRT
<213>  ˹(Artificial Sequence)

<220>
<223>  㶨


<400>  8
Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      
Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          
Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              
Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  
Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  
Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      
Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         
Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             
Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 
Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 
Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     
Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         
His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             
Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 
Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 
Leu Thr Lys Asn Gln Val Ser Leu Leu Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     
Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         
Asn Tyr Lys Thr Thr Pro Pro Val Leu Arg Ser Asp Gly Ser Phe Phe 
        275                 280                 285             
Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 
Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 
Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 

<210>  9
<211>  321
<212>  DNA
<213>  ˹(Artificial Sequence)

<220>
<223>  ɱ


<400>  9
gacatccaga tgacccagag ccctagcagc ctgagcgcca gcgtgggcga cagagtgacc        60
atcacctgca gagccagcca ggacatcagc aattacctga attggtacca gcagaagcct       120
ggcaaggtgc ctaagctgct gatctaccac accagcagac tgcacagcgg cgtgcctagc       180
agattcagcg gcagcggcag cggcaccgac ttcaccctga ccatcagcag cctgcagcct       240
gaggacgtgg ccacctacta ctgccagcag ggccacaccc tgcctagaac cttcggcggc       300
ggcaccaagg tggagatcaa g                                                 321

<210>  10
<211>  107
<212>  PRT
<213>  ˹(Artificial Sequence)

<220>
<223>  ɱ


<400>  10
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Ile Ser Asn Tyr 
            20                  25                  30          
Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Val Pro Lys Leu Leu Ile 
        35                  40                  45              
Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  
Glu Asp Val Ala Thr Tyr Tyr Cys Gln Gln Gly His Thr Leu Pro Arg 
                85                  90                  95      
Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         

<210>  11
<211>  357
<212>  DNA
<213>  ˹(Artificial Sequence)

<220>
<223>  ɱ


<400>  11
caggtgcagc tggtgcagag cggcgccgag gtgaagaagc ctggcagcag cgtgaaggtg        60
agctgcaagg ccagcggcta caccttcacc agctactgga tcaattgggt gagacaggct       120
ccgggccaag ggctggagtg gatgggcaat atctacccta gcgacagcta caccaattac       180
aatcagaagt tcaaggacag agtgaccatc accgccgaca agagcaccag caccgcctac       240
atggagctga gcagcctgag aagcgaggac accgccgtgt actactgcgc cagactgtac       300
tacggcagca gccctttcga ctactgggga caaggcacca ccgtgaccgt gagcagc          357

<210>  12
<211>  119
<212>  PRT
<213>  ˹(Artificial Sequence)

<220>
<223>  ɱ


<400>  12
Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      
Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          
Trp Ile Asn Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              
Gly Asn Ile Tyr Pro Ser Asp Ser Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  
Lys Asp Arg Val Thr Ile Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  
Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Arg Leu Tyr Tyr Gly Ser Ser Pro Phe Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         
Thr Thr Val Thr Val Ser Ser 
        115                 

<210>  13
<211>  990
<212>  DNA
<213>  ˹(Artificial Sequence)

<220>
<223>  㶨


<400>  13
gctagcacaa aaggaccttc cgtgtttcca ctggcaccct ctagtaagag tacttcagga        60
ggaaccgcag cactgggatg tctggtgaag gactacttcc cagagcccgt caccgtgtct       120
tggaacagtg gagcactgac ctccggggtc catacatttc ctgccgtgct gcagtcatcc       180
ggtctgtata gcctgagctc tgtggtcaca gtcccaagtt catccctggg cacccagaca       240
tacatctgca acgtgaatca caaaccttcc aatactaagg tcgacaagaa agtggaaccc       300
aagtcctgcg ataagaccca cacatgccct ccctgtcctg ctcccgaact gctgggagga       360
ccctccgtct tcctgttccc ccccaagccc aaagacacac tgatgatcag caggacccct       420
gaagtgacct gcgtggtcgt ggacgtgagc cacgaggacc ccgaggtcaa gtttaactgg       480
tacgtggacg gcgtggaggt ccacaacgcc aagaccaagc ccagggagga gcagtacgcc       540
agcacctaca gggtggtcag cgtgctgacc gtgctgcacc aggattggct caacggcaag       600
gagtacaagt gcaaagtctc caacaaggcc ctgcccgccc ccatcgagaa gaccatctcc       660
aaggctaagg gacagcccag ggagccccaa gtgtacaccg agcctcccag ccgggatgag       720
ctgaccaaga accaagtctc cctcacctgc ctggtcaagg gattctaccc ttccgacatt       780
gccgtcgaat gggagagcaa tggccagccc gagaacaact acaagacaac cccccccgtc       840
ctggatagcg acggatcctt cttcctgctc tccgtgctca ccgtcgacaa gagcagatgg       900
cagcagggca acgtgttcag ctgtagcgtg atgcacgagg ccctgcacaa ccactacacc       960
cagaagagcc tgtccctcag ccccggcaag                                        990

<210>  14
<211>  330
<212>  PRT
<213>  ˹(Artificial Sequence)

<220>
<223>  㶨


<400>  14
Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      
Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          
Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              
Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  
Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  
Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      
Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         
Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             
Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 
Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 
Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     
Glu Gln Tyr Ala Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         
His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             
Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 
Gln Pro Arg Glu Pro Gln Val Tyr Thr Glu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 
Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     
Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         
Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             
Leu Leu Ser Val Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 
Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 
Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 

<210>  15
<211>  10
<212>  PRT
<213>  ˹(Artificial Sequence)

<220>
<223>  PD-L1 HCDR1


<400>  15
Gly Phe Asn Ile Glu Asp Thr Tyr Ile His 
1               5                   10  

<210>  16
<211>  17
<212>  PRT
<213>  ˹(Artificial Sequence)

<220>
<223>  PD-L1 HCDR2


<400>  16
Arg Ile Asp Pro Ala Asn Ala Asn Thr Lys Tyr Asp Pro Lys Phe Gln 
1               5                   10                  15      
Gly 
    

<210>  17
<211>  8
<212>  PRT
<213>  ˹(Artificial Sequence)

<220>
<223>  PD-L1 HCDR3


<400>  17
Gly Leu Gly Ala Trp Phe Ala Tyr 
1               5               

<210>  18
<211>  11
<212>  PRT
<213>  ˹(Artificial Sequence)

<220>
<223>  PD-L1 LCDR1


<400>  18
Lys Ala Ser Gln Asp Val His Thr Ala Val Ala 
1               5                   10      

<210>  19
<211>  7
<212>  PRT
<213>  ˹(Artificial Sequence)

<220>
<223>  PD-L1 LCDR2


<400>  19
Ser Ala Ser Asn Arg Tyr Thr 
1               5           

<210>  20
<211>  9
<212>  PRT
<213>  ˹(Artificial Sequence)

<220>
<223>  PD-L1 LCDR3


<400>  20
Gln Gln His Tyr Ile Thr Pro Leu Thr 
1               5                   

<210>  21
<211>  10
<212>  PRT
<213>  ˹(Artificial Sequence)

<220>
<223>  4-1BB HCDR1


<400>  21
Gly Tyr Thr Phe Thr Ser Tyr Trp Ile Asn 
1               5                   10  

<210>  22
<211>  17
<212>  PRT
<213>  ˹(Artificial Sequence)

<220>
<223>  4-1BB HCDR2


<400>  22
Asn Ile Tyr Pro Ser Asp Ser Tyr Thr Asn Tyr Asn Gln Lys Phe Lys 
1               5                   10                  15      
Asp 
    

<210>  23
<211>  10
<212>  PRT
<213>  ˹(Artificial Sequence)

<220>
<223>  4-1BB HCDR3


<400>  23
Leu Tyr Tyr Gly Ser Ser Pro Phe Asp Tyr 
1               5                   10  

<210>  24
<211>  11
<212>  PRT
<213>  ˹(Artificial Sequence)

<220>
<223>  4-1BB LCDR1


<400>  24
Arg Ala Ser Gln Asp Ile Ser Asn Tyr Leu Asn 
1               5                   10      

<210>  25
<211>  7
<212>  PRT
<213>  ˹(Artificial Sequence)

<220>
<223>  4-1BB LCDR2


<400>  25
His Thr Ser Arg Leu His Ser 
1               5           

<210>  26
<211>  9
<212>  PRT
<213>  ˹(Artificial Sequence)

<220>
<223>  4-1BB LCDR3


<400>  26
Gln Gln Gly His Thr Leu Pro Arg Thr 
1               5                   

