﻿                         序列表

<110>  上海晟平医疗器械有限公司
       上海博璞诺科技发展有限公司
 
<120>  一种包含甲苯双加氧酶编码序列的多核苷酸和表达盒及其应用

<130>  P2021TC1906

<150>  CN202110015598.1
<151>  2021-01-06

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  3591
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  编码甲苯双加氧酶的多核苷酸

<400>  1
atgaaccaga ccgacacctc tccgatccgt ctgcgtcgtt cttggaacac ctctgaaatc       60

gaagctctgt tcgacgaaca cgctggtcgt atcgacccgc gtatctacac cgacgaagac      120

ctgtaccagc tggaactcga acgtgtcttc gcgcgttctt ggctgctgct gggtcacgaa      180

acccagatcc gtaaaccggg tgactacatc accacctaca tgggtgaaga cccggttgtt      240

gttgttcgtc agaaagacgc ttctatcgct gttttcctga accagtgccg tcaccgtggt      300

atgcgtatct gccgtgctga cgctggtaac gctaaagctt tcacctgctc ttaccacggt      360

tgggcttacg acaccgctgg taacctggtt aacgttccgt acgaagctga atctttcgct      420

tgcctgaaca aaaaagaatg gtctccgctg aaagctcgtg ttgaaaccta caaaggtctg      480

atcttcgcta actgggacga aaacgctgtt gacctggaca cctacctggg tgaagctaaa      540

ttctacatgg accacatgct ggaccgtacc gaagctggta ccgaagctat cccgggtgtt      600

cagaaatggg ttatcccgtg caactggaaa ttcgctgctg aacagttctg ctctgacatg      660

taccacgctg gtaccacctc tcacctgtct ggtatcctgg ctggtctgcc ggaagacctg      720

gaaatggctg acctggctcc gccgaccgtt ggtaaacagt accgtgcttc ttggggtggt      780

cacggttctg gtttctacgt tggtgacccg aacctgatgc tggctatcat gggtccgaaa      840

gttacctctt actggaccga aggtccggct tctgaaaaag ctgctgaacg tctgggttct      900

gttgaacgtg gttctaaact gatggttgaa cacatgaccg ttttcccgac ctgctctttc      960

ctgccgggta tcaacaccgt tcgtacctgg cacccgcgtg gtccgaacga ggttgaagta     1020

tgggcgttca ccgttgttga cgctgacgct ccggacgaca tcaaagaaga attccgtcgt     1080

cagaccctgc gtaccttctc tgctggtggt gttttcgaac aggacgacgg tgaaaactgg     1140

gttgaaatcc agcacatcct gcgtggtcac aaagctcgtt ctcgtccgtt caacgctgaa     1200

atgtctatgg accagaccgt tgacaacgac ccggtttacc cgggtcgtat ctctaacaac     1260

gtttactctg aagaagctgc tcgtggtctg tacgctcact ggctgcgtat gatgacctct     1320

ccggactggg acgctctgaa agctacccgt taatcgagat ctcgatcccg cgaaattaat     1380

acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt     1440

taactttaag aaggagatat accatgatcg actctgctaa ccgtgctgac gttttcctgc     1500

gtaaaccggc tccggttgct ccggaactgc agcacgaagt tgaacagttc tactactggg     1560

aagctaaact gctgaacgac cgtcgtttcg aagaatggtt cgctctgctg gctgaagaca     1620

tccactactt catgccgatc cgtaccaccc gtatcatgcg tgactctcgt ctggaatact     1680

ctggttctcg tgaatacgct cacttcgacg acgacgctac catgatgaaa ggtcgtctgc     1740

gtaaaatcac ctctgacgtt tcttggtctg aaaacccggc ttctcgtacc cgtcacctgg     1800

tttctaacgt tatgatcgtt ggtgctgaag ctgaaggtga atacgaaatc tcttctgctt     1860

tcatcgttta ccgtaaccgt ctggaacgtc agctggacat cttcgctggt gaacgtcgtg     1920

acaccctgcg tcgtaacacc tctgaagctg gtttcgaaat cgttaaccgt accatcctga     1980

tcgaccagtc taccatcctg gctaacaacc tgtctttctt cttctaagtg atgtcatgac     2040

ctggacctac atcctgcgtc agggtgacct gccgccgggt gaaatgcagc gttacgaagg     2100

tggtccggaa ccggttatgg tttgcaacgt tgacggtgaa tttttcgctg ttcaggacac     2160

ctgcacccac ggtgactggg ctctgtctga cggttacctg gacggtgaca tcgttgaatg     2220

caccctgcac ttcggtaaat tctgcgttcg taccggtaaa gttaaagctc tgccggcttg     2280

caaaccgatc aaagttttcc cgatcaaagt tgaaggtgac gaagttcacg ttgacctgga     2340

caacggtgaa ctgaaataat ggctacccac gttgctatca tcggtaacgg cgttggtggc     2400

ttcaccaccg ctcaggctct gcgtgcggaa ggcttcgaag gtcgtatatc tctgatcggt     2460

gacgaaccgc acctgccgta cgaccgtccg tctctgtcta aagctgttct ggacggttct     2520

ctggaacgtc cgccgatcct ggctgaagct gactggtacg gtgaagctcg tatcgacatg     2580

ctgaccggtc cggaagttac cgctctggac gttcagaccc gtaccatctc tctggacgac     2640

ggtaccaccc tgtctgctga cgctatcgtt atcgctaccg gttctcgtgc tcgtactatg     2700

gctctgccgg gttctcagct gccgggtgtt gttaccctgc gtacctacgg tgacgttcag     2760

gttctgcgtg actcttggac ctctgctacc cgtctgctga tcgttggtgg tggtctgatc     2820

ggttgcgaag ttgctaccac cgctcgtaaa ctgggtctgt ctgttaccat cctggaagct     2880

ggtgacgaac tgctggttcg tgttctgggt cgtcgtatcg gtgcttggct gcgtggtctg     2940

ctgaccgaac tgggtgttca ggttgaactg ggtaccggtg ttgttggttt ctctggtgaa     3000

ggtcagctgg aacaggttat ggcttctgac ggtcgttctt tcgttgctga ctctgctctg     3060

atctgcgttg gtgctgaacc ggctgaccag ctggctcgtc aggctggtct ggcttgcgac     3120

cgtggtgtta tcgttgacca ctgcggtgct accctggcta aaggtgtttt cgctgttggt     3180

gacgttgctt cttggccgct gcgtgctggt ggtcgtcgtt ctctggaaac ctacatgaac     3240

gctcagcgtc aggctgctgc tgttgctgct gctatcctgg gtaaaaacgt ttctgcgccg     3300

cagctgccgg tgagctggac cgaaatcgct ggtcaccgta tgcagatggc tggtgacatc     3360

gaaggtccgg gtgacttcgt ttctcgtggt atgccgggtt ctggtgctgc tctgctgttc     3420

cgtctgcaag aacgtcgtat ccaggctgtt gttgctgttg acgctccgcg tgacttcgct     3480

ctggctaccc gtctggttga agctcgtgct gctatcgaac cggctcgtct ggctgacctg     3540

tctaactcta tgcgtgactt cgttcgtgct aacgaaggtg acctgaccta a              3591


<210>  2
<211>  3597
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  编码甲苯双加氧酶的多核苷酸

<400>  2
atgaaccaga ccgacacctc tccgatccgt ctgcgtcgtt cttggaacac ctctgaaatc       60

gaagctctgt tcgacgaaca cgctggtcgt atcgacccgc gtatctacac cgacgaagac      120

ctgtaccagc tggaactcga acgtgtcttc gcgcgttctt ggctgctgct gggtcacgaa      180

acccagatcc gtaaaccggg tgactacatc accacctaca tgggtgaaga cccggttgtt      240

gttgttcgtc agaaagacgc ttctatcgct gttttcctga accagtgccg tcaccgtggt      300

atgcgtatct gccgtgctga cgctggtaac gctaaagctt tcacctgctc ttaccacggt      360

tgggcttacg acaccgctgg taacctggtt aacgttccgt acgaagctga atctttcgct      420

tgcctgaaca aaaaagaatg gtctccgctg aaagctcgtg ttgaaaccta caaaggtctg      480

atcttcgcta actgggacga aaacgctgtt gacctggaca cctacctggg tgaagctaaa      540

ttctacatgg accacatgct ggaccgtacc gaagctggta ccgaagctat cccgggtgtt      600

cagaaatggg ttatcccgtg caactggaaa ttcgctgctg aacagttctg ctctgacatg      660

taccacgctg gtaccacctc tcacctgtct ggtatcctgg ctggtctgcc ggaagacctg      720

gaaatggctg acctggctcc gccgaccgtt ggtaaacagt accgtgcttc ttggggtggt      780

cacggttctg gtttctacgt tggtgacccg aacctgatgc tggctatcat gggtccgaaa      840

gttacctctt actggaccga aggtccggct tctgaaaaag ctgctgaacg tctgggttct      900

gttgaacgtg gttctaaact gatggttgaa cacatgaccg ttttcccgac ctgctctttc      960

ctgccgggta tcaacaccgt tcgtacctgg cacccgcgtg gtccgaacga ggttgaagta     1020

tgggcgttca ccgttgttga cgctgacgct ccggacgaca tcaaagaaga attccgtcgt     1080

cagaccctgc gtaccttctc tgctggtggt gttttcgaac aggacgacgg tgaaaactgg     1140

gttgaaatcc agcacatcct gcgtggtcac aaagctcgtt ctcgtccgtt caacgctgaa     1200

atgtctatgg accagaccgt tgacaacgac ccggtttacc cgggtcgtat ctctaacaac     1260

gtttactctg aagaagctgc tcgtggtctg tacgctcact ggctgcgtat gatgacctct     1320

ccggactggg acgctctgaa agctacccgt taatcgagat ctcgatcccg cgaaattaat     1380

acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt     1440

taactttaag aaggagatat accatgatcg actctgctaa ccgtgctgac gttttcctgc     1500

gtaaaccggc tccggttgct ccggaactgc agcacgaagt tgaacagttc tactactggg     1560

aagctaaact gctgaacgac cgtcgtttcg aagaatggtt cgctctgctg gctgaagaca     1620

tccactactt catgccgatc cgtaccaccc gtatcatgcg tgactctcgt ctggaatact     1680

ctggttctcg tgaatacgct cacttcgacg acgacgctac catgatgaaa ggtcgtctgc     1740

gtaaaatcac ctctgacgtt tcttggtctg aaaacccggc ttctcgtacc cgtcacctgg     1800

tttctaacgt tatgatcgtt ggtgctgaag ctgaaggtga atacgaaatc tcttctgctt     1860

tcatcgttta ccgtaaccgt ctggaacgtc agctggacat cttcgctggt gaacgtcgtg     1920

acaccctgcg tcgtaacacc tctgaagctg gtttcgaaat cgttaaccgt accatcctga     1980

tcgaccagtc taccatcctg gctaacaacc tgtctttctt cttctaagaa ggagatatac     2040

catgacctgg acctacatcc tgcgtcaggg tgacctgccg ccgggtgaaa tgcagcgtta     2100

cgaaggtggt ccggaaccgg ttatggtttg caacgttgac ggtgaatttt tcgctgttca     2160

ggacacctgc acccacggtg actgggctct gtctgacggt tacctggacg gtgacatcgt     2220

tgaatgcacc ctgcacttcg gtaaattctg cgttcgtacc ggtaaagtta aagctctgcc     2280

ggcttgcaaa ccgatcaaag ttttcccgat caaagttgaa ggtgacgaag ttcacgttga     2340

cctggacaac ggtgaactga aataatggct acccacgttg ctatcatcgg taacggcgtt     2400

ggtggcttca ccaccgctca ggctctgcgt gcggaaggct tcgaaggtcg tatatctctg     2460

atcggtgacg aaccgcacct gccgtacgac cgtccgtctc tgtctaaagc tgttctggac     2520

ggttctctgg aacgtccgcc gatcctggct gaagctgact ggtacggtga agctcgtatc     2580

gacatgctga ccggtccgga agttaccgct ctggacgttc agacccgtac catctctctg     2640

gacgacggta ccaccctgtc tgctgacgct atcgttatcg ctaccggttc tcgtgctcgt     2700

actatggctc tgccgggttc tcagctgccg ggtgttgtta ccctgcgtac ctacggtgac     2760

gttcaggttc tgcgtgactc ttggacctct gctacccgtc tgctgatcgt tggtggtggt     2820

ctgatcggtt gcgaagttgc taccaccgct cgtaaactgg gtctgtctgt taccatcctg     2880

gaagctggtg acgaactgct ggttcgtgtt ctgggtcgtc gtatcggtgc ttggctgcgt     2940

ggtctgctga ccgaactggg tgttcaggtt gaactgggta ccggtgttgt tggtttctct     3000

ggtgaaggtc agctggaaca ggttatggct tctgacggtc gttctttcgt tgctgactct     3060

gctctgatct gcgttggtgc tgaaccggct gaccagctgg ctcgtcaggc tggtctggct     3120

tgcgaccgtg gtgttatcgt tgaccactgc ggtgctaccc tggctaaagg tgttttcgct     3180

gttggtgacg ttgcttcttg gccgctgcgt gctggtggtc gtcgttctct ggaaacctac     3240

atgaacgctc agcgtcaggc tgctgctgtt gctgctgcta tcctgggtaa aaacgtttct     3300

gcgccgcagc tgccggtgag ctggaccgaa atcgctggtc accgtatgca gatggctggt     3360

gacatcgaag gtccgggtga cttcgtttct cgtggtatgc cgggttctgg tgctgctctg     3420

ctgttccgtc tgcaagaacg tcgtatccag gctgttgttg ctgttgacgc tccgcgtgac     3480

ttcgctctgg ctacccgtct ggttgaagct cgtgctgcta tcgaaccggc tcgtctggct     3540

gacctgtcta actctatgcg tgacttcgtt cgtgctaacg aaggtgacct gacctaa        3597


<210>  3
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  第一间隔序列

<400>  3
tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa       60

caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc                 110


<210>  4
<211>  450
<212>  PRT
<213>  恶臭假单胞菌（Pseudomonas putida）

<220>  
<223>  TodC1

<400>  4

Met Asn Gln Thr Asp Thr Ser Pro Ile Arg Leu Arg Arg Ser Trp Asn 
1               5                   10                  15      


Thr Ser Glu Ile Glu Ala Leu Phe Asp Glu His Ala Gly Arg Ile Asp 
            20                  25                  30          


Pro Arg Ile Tyr Thr Asp Glu Asp Leu Tyr Gln Leu Glu Leu Glu Arg 
        35                  40                  45              


Val Phe Ala Arg Ser Trp Leu Leu Leu Gly His Glu Thr Gln Ile Arg 
    50                  55                  60                  


Lys Pro Gly Asp Tyr Ile Thr Thr Tyr Met Gly Glu Asp Pro Val Val 
65                  70                  75                  80  


Val Val Arg Gln Lys Asp Ala Ser Ile Ala Val Phe Leu Asn Gln Cys 
                85                  90                  95      


Arg His Arg Gly Met Arg Ile Cys Arg Ala Asp Ala Gly Asn Ala Lys 
            100                 105                 110         


Ala Phe Thr Cys Ser Tyr His Gly Trp Ala Tyr Asp Thr Ala Gly Asn 
        115                 120                 125             


Leu Val Asn Val Pro Tyr Glu Ala Glu Ser Phe Ala Cys Leu Asn Lys 
    130                 135                 140                 


Lys Glu Trp Ser Pro Leu Lys Ala Arg Val Glu Thr Tyr Lys Gly Leu 
145                 150                 155                 160 


Ile Phe Ala Asn Trp Asp Glu Asn Ala Val Asp Leu Asp Thr Tyr Leu 
                165                 170                 175     


Gly Glu Ala Lys Phe Tyr Met Asp His Met Leu Asp Arg Thr Glu Ala 
            180                 185                 190         


Gly Thr Glu Ala Ile Pro Gly Val Gln Lys Trp Val Ile Pro Cys Asn 
        195                 200                 205             


Trp Lys Phe Ala Ala Glu Gln Phe Cys Ser Asp Met Tyr His Ala Gly 
    210                 215                 220                 


Thr Thr Ser His Leu Ser Gly Ile Leu Ala Gly Leu Pro Glu Asp Leu 
225                 230                 235                 240 


Glu Met Ala Asp Leu Ala Pro Pro Thr Val Gly Lys Gln Tyr Arg Ala 
                245                 250                 255     


Ser Trp Gly Gly His Gly Ser Gly Phe Tyr Val Gly Asp Pro Asn Leu 
            260                 265                 270         


Met Leu Ala Ile Met Gly Pro Lys Val Thr Ser Tyr Trp Thr Glu Gly 
        275                 280                 285             


Pro Ala Ser Glu Lys Ala Ala Glu Arg Leu Gly Ser Val Glu Arg Gly 
    290                 295                 300                 


Ser Lys Leu Met Val Glu His Met Thr Val Phe Pro Thr Cys Ser Phe 
305                 310                 315                 320 


Leu Pro Gly Ile Asn Thr Val Arg Thr Trp His Pro Arg Gly Pro Asn 
                325                 330                 335     


Glu Val Glu Val Trp Ala Phe Thr Val Val Asp Ala Asp Ala Pro Asp 
            340                 345                 350         


Asp Ile Lys Glu Glu Phe Arg Arg Gln Thr Leu Arg Thr Phe Ser Ala 
        355                 360                 365             


Gly Gly Val Phe Glu Gln Asp Asp Gly Glu Asn Trp Val Glu Ile Gln 
    370                 375                 380                 


His Ile Leu Arg Gly His Lys Ala Arg Ser Arg Pro Phe Asn Ala Glu 
385                 390                 395                 400 


Met Ser Met Asp Gln Thr Val Asp Asn Asp Pro Val Tyr Pro Gly Arg 
                405                 410                 415     


Ile Ser Asn Asn Val Tyr Ser Glu Glu Ala Ala Arg Gly Leu Tyr Ala 
            420                 425                 430         


His Trp Leu Arg Met Met Thr Ser Pro Asp Trp Asp Ala Leu Lys Ala 
        435                 440                 445             


Thr Arg 
    450 


<210>  5
<211>  187
<212>  PRT
<213>  恶臭假单胞菌（Pseudomonas putida）

<220>  
<223>  TodC2

<400>  5

Met Ile Asp Ser Ala Asn Arg Ala Asp Val Phe Leu Arg Lys Pro Ala 
1               5                   10                  15      


Pro Val Ala Pro Glu Leu Gln His Glu Val Glu Gln Phe Tyr Tyr Trp 
            20                  25                  30          


Glu Ala Lys Leu Leu Asn Asp Arg Arg Phe Glu Glu Trp Phe Ala Leu 
        35                  40                  45              


Leu Ala Glu Asp Ile His Tyr Phe Met Pro Ile Arg Thr Thr Arg Ile 
    50                  55                  60                  


Met Arg Asp Ser Arg Leu Glu Tyr Ser Gly Ser Arg Glu Tyr Ala His 
65                  70                  75                  80  


Phe Asp Asp Asp Ala Thr Met Met Lys Gly Arg Leu Arg Lys Ile Thr 
                85                  90                  95      


Ser Asp Val Ser Trp Ser Glu Asn Pro Ala Ser Arg Thr Arg His Leu 
            100                 105                 110         


Val Ser Asn Val Met Ile Val Gly Ala Glu Ala Glu Gly Glu Tyr Glu 
        115                 120                 125             


Ile Ser Ser Ala Phe Ile Val Tyr Arg Asn Arg Leu Glu Arg Gln Leu 
    130                 135                 140                 


Asp Ile Phe Ala Gly Glu Arg Arg Asp Thr Leu Arg Arg Asn Thr Ser 
145                 150                 155                 160 


Glu Ala Gly Phe Glu Ile Val Asn Arg Thr Ile Leu Ile Asp Gln Ser 
                165                 170                 175     


Thr Ile Leu Ala Asn Asn Leu Ser Phe Phe Phe 
            180                 185         


<210>  6
<211>  107
<212>  PRT
<213>  恶臭假单胞菌（Pseudomonas putida）

<220>  
<223>  TodB

<400>  6

Met Thr Trp Thr Tyr Ile Leu Arg Gln Gly Asp Leu Pro Pro Gly Glu 
1               5                   10                  15      


Met Gln Arg Tyr Glu Gly Gly Pro Glu Pro Val Met Val Cys Asn Val 
            20                  25                  30          


Asp Gly Glu Phe Phe Ala Val Gln Asp Thr Cys Thr His Gly Asp Trp 
        35                  40                  45              


Ala Leu Ser Asp Gly Tyr Leu Asp Gly Asp Ile Val Glu Cys Thr Leu 
    50                  55                  60                  


His Phe Gly Lys Phe Cys Val Arg Thr Gly Lys Val Lys Ala Leu Pro 
65                  70                  75                  80  


Ala Cys Lys Pro Ile Lys Val Phe Pro Ile Lys Val Glu Gly Asp Glu 
                85                  90                  95      


Val His Val Asp Leu Asp Asn Gly Glu Leu Lys 
            100                 105         


<210>  7
<211>  410
<212>  PRT
<213>  恶臭假单胞菌（Pseudomonas putida）

<220>  
<223>  TodA

<400>  7

Met Ala Thr His Val Ala Ile Ile Gly Asn Gly Val Gly Gly Phe Thr 
1               5                   10                  15      


Thr Ala Gln Ala Leu Arg Ala Glu Gly Phe Glu Gly Arg Ile Ser Leu 
            20                  25                  30          


Ile Gly Asp Glu Pro His Leu Pro Tyr Asp Arg Pro Ser Leu Ser Lys 
        35                  40                  45              


Ala Val Leu Asp Gly Ser Leu Glu Arg Pro Pro Ile Leu Ala Glu Ala 
    50                  55                  60                  


Asp Trp Tyr Gly Glu Ala Arg Ile Asp Met Leu Thr Gly Pro Glu Val 
65                  70                  75                  80  


Thr Ala Leu Asp Val Gln Thr Arg Thr Ile Ser Leu Asp Asp Gly Thr 
                85                  90                  95      


Thr Leu Ser Ala Asp Ala Ile Val Ile Ala Thr Gly Ser Arg Ala Arg 
            100                 105                 110         


Thr Met Ala Leu Pro Gly Ser Gln Leu Pro Gly Val Val Thr Leu Arg 
        115                 120                 125             


Thr Tyr Gly Asp Val Gln Val Leu Arg Asp Ser Trp Thr Ser Ala Thr 
    130                 135                 140                 


Arg Leu Leu Ile Val Gly Gly Gly Leu Ile Gly Cys Glu Val Ala Thr 
145                 150                 155                 160 


Thr Ala Arg Lys Leu Gly Leu Ser Val Thr Ile Leu Glu Ala Gly Asp 
                165                 170                 175     


Glu Leu Leu Val Arg Val Leu Gly Arg Arg Ile Gly Ala Trp Leu Arg 
            180                 185                 190         


Gly Leu Leu Thr Glu Leu Gly Val Gln Val Glu Leu Gly Thr Gly Val 
        195                 200                 205             


Val Gly Phe Ser Gly Glu Gly Gln Leu Glu Gln Val Met Ala Ser Asp 
    210                 215                 220                 


Gly Arg Ser Phe Val Ala Asp Ser Ala Leu Ile Cys Val Gly Ala Glu 
225                 230                 235                 240 


Pro Ala Asp Gln Leu Ala Arg Gln Ala Gly Leu Ala Cys Asp Arg Gly 
                245                 250                 255     


Val Ile Val Asp His Cys Gly Ala Thr Leu Ala Lys Gly Val Phe Ala 
            260                 265                 270         


Val Gly Asp Val Ala Ser Trp Pro Leu Arg Ala Gly Gly Arg Arg Ser 
        275                 280                 285             


Leu Glu Thr Tyr Met Asn Ala Gln Arg Gln Ala Ala Ala Val Ala Ala 
    290                 295                 300                 


Ala Ile Leu Gly Lys Asn Val Ser Ala Pro Gln Leu Pro Val Ser Trp 
305                 310                 315                 320 


Thr Glu Ile Ala Gly His Arg Met Gln Met Ala Gly Asp Ile Glu Gly 
                325                 330                 335     


Pro Gly Asp Phe Val Ser Arg Gly Met Pro Gly Ser Gly Ala Ala Leu 
            340                 345                 350         


Leu Phe Arg Leu Gln Glu Arg Arg Ile Gln Ala Val Val Ala Val Asp 
        355                 360                 365             


Ala Pro Arg Asp Phe Ala Leu Ala Thr Arg Leu Val Glu Ala Arg Ala 
    370                 375                 380                 


Ala Ile Glu Pro Ala Arg Leu Ala Asp Leu Ser Asn Ser Met Arg Asp 
385                 390                 395                 400 


Phe Val Arg Ala Asn Glu Gly Asp Leu Thr 
                405                 410 


<210>  8
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7启动子

<400>  8
taatacgact cactatagg                                                    19


<210>  9
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  原核核糖体结合位点

<400>  9
gaaggagata tacc                                                          14


<210>  10
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lac操纵基因

<400>  10
ttgtgagcgg ataacaa                                                      17


<210>  11
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7转录终止子

<400>  11
ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttg                    48

<210>  12
<211>  3591
<212>  DNA
<213>  恶臭假单胞菌（Pseudomonas putida）

<220>  
<223>  J04996.1中第620-4210位核苷酸

<400>  12
atgaatcaga ccgacacatc acctatcagg ctgcgcagga gctggaacac cagcgagata       60

gaagcgctct ttgacgagca tgccggacgt atcgatccgc gcatttatac cgatgaggat      120

ctgtaccaac tcgaactgga gcgtgtcttc gcccggtcct ggctgctgtt ggggcatgaa      180

acccagattc gcaagccggg cgattacatc acgacctaca tgggtgaaga ccctgtcgtg      240

gtcgtccggc agaaagacgc cagcattgcc gtgttcctga accagtgccg ccaccgtggc      300

atgcgcatct gccgcgcgga tgccggaaac gcgaaggcgt tcacttgcag ctaccacggg      360

tgggcttacg acaccgccgg caatcttgtc aatgtgcctt acgaggccga atccttcgcg      420

tgcctgaaca agaaggaatg gagcccgctg aaggcccggg tagaaaccta caagggcctg      480

attttcgcca actgggatga gaacgctgta gacctcgaca cgtatctggg cgaggcgaag      540

ttctacatgg accacatgct cgaccgcacc gaggccggca ccgaagcgat cccgggcgtg      600

cagaagtggg tcattccctg taactggaaa ttcgccgcag agcagttttg cagcgacatg      660

taccatgccg ggacgacctc gcatctgtct ggcatcctgg caggcctgcc agaagacctt      720

gaaatggccg accttgctcc gccgacagtt ggcaagcagt accgtgcgtc atggggcgga      780

catggaagtg gcttctatgt cggcgacccc aatctgatgc ttgccatcat ggggccaaag      840

gtcaccagct actggaccga aggccccgcg tcggaaaagg cggccgaacg tctgggtagc      900

gtggagcgcg gctcgaaact catggtcgag cacatgaccg tcttccccac gtgttccttc      960

ctcccaggta tcaatacggt ccggacatgg catccgcgcg ggccgaacga ggtcgaggta     1020

tgggcgttta cggtggtcga tgctgatgct cctgacgata tcaaggaaga gttccggcgc     1080

cagacgctgc gcaccttctc tgccggtggc gtgttcgagc aggacgacgg ggagaactgg     1140

gtcgagatcc agcacatcct gcgaggccac aaggcgcgga gccgcccttt caatgccgag     1200

atgagcatgg accagaccgt cgacaacgac ccggtttacc ccgggcggat cagcaacaac     1260

gtctacagcg aggaagctgc ccgcgggctc tatgcccatt ggctgcggat gatgacatcc     1320

cccgactggg acgcgctgaa ggcgacacgc tgaatccaga gacagcttgc gccacgcagt     1380

ggcgccggcc agaggccgca tttgacttcg acccaggttg gatgcggtgg accttgtcca     1440

tttgaaatct acaaggaacg accatgattg attcagccaa cagagccgac gtctttctcc     1500

gcaagccggc acccgtagcg cccgaactgc agcacgaagt cgagcagttc tactattggg     1560

aggccaagct tctcaacgat cgccgcttcg aggagtggtt cgcgctgctc gcggaagaca     1620

ttcactactt catgcccatt cgcaccacgc ggatcatgcg ggactcgcgc cttgaatact     1680

caggctcccg agagtacgcg cacttcgatg acgacgccac gatgatgaag ggacgcttgc     1740

gcaagatcac gtccgacgtg agctggtccg agaaccccgc atcgcggacc cggcatctcg     1800

tgagcaacgt gatgatcgtc ggcgcagagg cagaagggga gtacgaaatc tcaagcgcct     1860

tcattgtgta ccgcaatcgt ctggagcggc agctcgacat ctttgccggt gagcgtcgcg     1920

atacgttgcg ccgtaacacg agcgaggccg ggttcgagat cgtcaatcgg accatcctga     1980

tcgaccagag caccatcctg gccaataacc tcagtttctt cttctaggtg atgtcatgac     2040

ttggacatac atattgcggc agggtgacct gccacccggt gagatgcagc gctacgaagg     2100

cggcccggaa cctgtgatgg tctgcaacgt cgatggcgag ttcttcgcgg tgcaggatac     2160

ctgcacgcat ggggactggg cgttgtcgga tggttacctg gacggtgata ttgtcgaatg     2220

cacgttgcat ttcggcaagt tctgcgtgcg gaccgggaag gtgaaggcgc tgcctgcttg     2280

caaacctatc aaggtattcc caatcaaggt cgaaggcgat gaagtgcacg tcgatctcga     2340

caacggggag ttgaagtgat ggctacccat gtggcgatca tcggcaatgg cgtgggtggc     2400

ttcacgaccg cgcaggccct acgtgccgag ggcttcgagg ggagaatctc gctgattggg     2460

gacgaaccgc atctccccta tgaccgacca tccttgtcca aggcggttct cgacggcagc     2520

cttgagcggc cgcccatact ggccgaggcc gattggtacg gcgaggcccg catcgacatg     2580

ctgaccggcc cggaagtcac tgcccttgat gtgcagacaa ggacgatcag tctggatgat     2640

ggcaccacgc tctctgcgga cgccatcgtc atcgcgacgg gcagtcgagc gcggacgatg     2700

gcgttgcccg gcagccaact gcccggcgtc gtaacgctgc gcacctacgg tgacgtgcag     2760

gtattgcgcg atagttggac ttccgcgacg cggctgctga ttgtgggtgg cggattgatc     2820

ggctgcgagg tcgcgacgac ggcgcgcaag ctcggcctgt cggtcacgat cctggaggca     2880

ggtgatgaac tgctggtccg agtacttggg cggcgtatcg gtgcctggct gcgcggcctg     2940

ctgacagaac ttggtgtgca ggtcgagttg ggaacgggtg tcgtaggttt ttctggtgag     3000

ggccagctcg aacaagtcat ggccagcgat gggcgcagct tcgtagccga tagcgcactc     3060

atttgcgtcg gcgcggagcc cgcggatcaa cttgcgcgtc aagcgggctt ggcatgtgac     3120

cgcggcgtca ttgtcgatca ctgcggtgcg acgcttgcca aaggcgtatt cgccgtcgga     3180

gatgtggcca gttggccgct gcgcgccggc ggccggcgtt cgctcgaaac ctatatgaac     3240

gcgcagcgcc aagccgccgc ggtggctgcg gccattctgg ggaaaaacgt atcggcaccg     3300

caactgcccg tgtcctggac ggagatcgct gggcatcgca tgcagatggc gggcgatatc     3360

gaaggacctg gtgatttcgt ctcgcgcggc atgcccggta gtggcgctgc cctgttgttc     3420

cgcctgcagg agcgaaggat tcaggcggtc gtcgcggtcg atgcaccccg tgacttcgcg     3480

cttgcaaccc gattggtaga agcccgcgcg gcaatcgagc cagcacggct ggcagatctt     3540

tcaaacagta tgcgcgattt tgttcgtgcg aatgaaggag acctaacgtg a              3591


<210>  13
<211>  3590
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  编码甲苯双加氧酶的多核苷酸

<400>  13
atgaaccaga ccgacacctc tccgatccgt ctgcgtcgtt cttggaacac ctctgaaatc       60

gaagctctgt tcgacgaaca cgctggtcgt atcgacccgc gtatctacac cgacgaagac      120

ctgtaccagc tggaactcga acgtgtcttc gcgcgttctt ggctgctgct gggtcacgaa      180

acccagatcc gtaaaccggg tgactacatc accacctaca tgggtgaaga cccggttgtt      240

gttgttcgtc agaaagacgc ttctatcgct gttttcctga accagtgccg tcaccgtggt      300

atgcgtatct gccgtgctga cgctggtaac gctaaagctt tcacctgctc ttaccacggt      360

tgggcttacg acaccgctgg taacctggtt aacgttccgt acgaagctga atctttcgct      420

tgcctgaaca aaaaagaatg gtctccgctg aaagctcgtg ttgaaaccta caaaggtctg      480

atcttcgcta actgggacga aaacgctgtt gacctggaca cctacctggg tgaagctaaa      540

ttctacatgg accacatgct ggaccgtacc gaagctggta ccgaagctat cccgggtgtt      600

cagaaatggg ttatcccgtg caactggaaa ttcgctgctg aacagttctg ctctgacatg      660

taccacgctg gtaccacctc tcacctgtct ggtatcctgg ctggtctgcc ggaagacctg      720

gaaatggctg acctggctcc gccgaccgtt ggtaaacagt accgtgcttc ttggggtggt      780

cacggttctg gtttctacgt tggtgacccg aacctgatgc tggctatcat gggtccgaaa      840

gttacctctt actggaccga aggtccggct tctgaaaaag ctgctgaacg tctgggttct      900

gttgaacgtg gttctaaact gatggttgaa cacatgaccg ttttcccgac ctgctctttc      960

ctgccgggta tcaacaccgt tcgtacctgg cacccgcgtg gtccgaacga ggttgaagta     1020

tgggcgttca ccgttgttga cgctgacgct ccggacgaca tcaaagaaga attccgtcgt     1080

cagaccctgc gtaccttctc tgctggtggt gttttcgaac aggacgacgg tgaaaactgg     1140

gttgaaatcc agcacatcct gcgtggtcac aaagctcgtt ctcgtccgtt caacgctgaa     1200

atgtctatgg accagaccgt tgacaacgac ccggtttacc cgggtcgtat ctctaacaac     1260

gtttactctg aagaagctgc tcgtggtctg tacgctcact ggctgcgtat gatgacctct     1320

ccggactggg acgctctgaa agctacccgt taatccagag acagcttgcg ccacgcagtg     1380

gcgccggcca gaggccgcat ttgacttcga cccaggttgg atgcggtgga ccttgtccat     1440

ttgaaatcta caaggaacga ccatgatcga ctctgctaac cgtgctgacg ttttcctgcg     1500

taaaccggct ccggttgctc cggaactgca gcacgaagtt gaacagttct actactggga     1560

agctaaactg ctgaacgacc gtcgtttcga agaatggttc gctctgctgg ctgaagacat     1620

ccactacttc atgccgatcc gtaccacccg tatcatgcgt gactctcgtc tggaatactc     1680

tggttctcgt gaatacgctc acttcgacga cgacgctacc atgatgaaag gtcgtctgcg     1740

taaaatcacc tctgacgttt cttggtctga aaacccggct tctcgtaccc gtcacctggt     1800

ttctaacgtt atgatcgttg gtgctgaagc tgaaggtgaa tacgaaatct cttctgcttt     1860

catcgtttac cgtaaccgtc tggaacgtca gctggacatc ttcgctggtg aacgtcgtga     1920

caccctgcgt cgtaacacct ctgaagctgg tttcgaaatc gttaaccgta ccatcctgat     1980

cgaccagtct accatcctgg ctaacaacct gtctttcttc ttctaagtga tgtcatgacc     2040

tggacctaca tcctgcgtca gggtgacctg ccgccgggtg aaatgcagcg ttacgaaggt     2100

ggtccggaac cggttatggt ttgcaacgtt gacggtgaat ttttcgctgt tcaggacacc     2160

tgcacccacg gtgactgggc tctgtctgac ggttacctgg acggtgacat cgttgaatgc     2220

accctgcact tcggtaaatt ctgcgttcgt accggtaaag ttaaagctct gccggcttgc     2280

aaaccgatca aagttttccc gatcaaagtt gaaggtgacg aagttcacgt tgacctggac     2340

aacggtgaac tgaaataatg gctacccacg ttgctatcat cggtaacggc gttggtggct     2400

tcaccaccgc tcaggctctg cgtgcggaag gcttcgaagg tcgtatatct ctgatcggtg     2460

acgaaccgca cctgccgtac gaccgtccgt ctctgtctaa agctgttctg gacggttctc     2520

tggaacgtcc gccgatcctg gctgaagctg actggtacgg tgaagctcgt atcgacatgc     2580

tgaccggtcc ggaagttacc gctctggacg ttcagacccg taccatctct ctggacgacg     2640

gtaccaccct gtctgctgac gctatcgtta tcgctaccgg ttctcgtgct cgtactatgg     2700

ctctgccggg ttctcagctg ccgggtgttg ttaccctgcg tacctacggt gacgttcagg     2760

ttctgcgtga ctcttggacc tctgctaccc gtctgctgat cgttggtggt ggtctgatcg     2820

gttgcgaagt tgctaccacc gctcgtaaac tgggtctgtc tgttaccatc ctggaagctg     2880

gtgacgaact gctggttcgt gttctgggtc gtcgtatcgg tgcttggctg cgtggtctgc     2940

tgaccgaact gggtgttcag gttgaactgg gtaccggtgt tgttggtttc tctggtgaag     3000

gtcagctgga acaggttatg gcttctgacg gtcgttcttt cgttgctgac tctgctctga     3060

tctgcgttgg tgctgaaccg gctgaccagc tggctcgtca ggctggtctg gcttgcgacc     3120

gtggtgttat cgttgaccac tgcggtgcta ccctggctaa aggtgttttc gctgttggtg     3180

acgttgcttc ttggccgctg cgtgctggtg gtcgtcgttc tctggaaacc tacatgaacg     3240

ctcagcgtca ggctgctgct gttgctgctg ctatcctggg taaaaacgtt tctgcgccgc     3300

agctgccggt gagctggacc gaaatcgctg gtcaccgtat gcagatggct ggtgacatcg     3360

aaggtccggg tgacttcgtt tctcgtggta tgccgggttc tggtgctgct ctgctgttcc     3420

gtctgcaaga acgtcgtatc caggctgttg ttgctgttga cgctccgcgt gacttcgctc     3480

tggctacccg tctggttgaa gctcgtgctg ctatcgaacc ggctcgtctg gctgacctgt     3540

ctaactctat gcgtgacttc gttcgtgcta acgaaggtga cctgacctaa                3590

