                         SEQUENCE LISTING

<110>  The University of North Carolina at Chapel Hill
       Xiao, Xiao
       Qiao, Chunping
 
<120>  Optimized Fukutin-Related Proteins (FKRP) and Methods of Use

<130>  5470.874.WO

<150>  63/133,615
<151>  2021-01-04

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  1491
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  opti-hu-FKRP-CpG(-)

<400>  1
atgagactga caagatgtca agctgccctg gctgctgcca tcacactgaa tctgctggtg       60

ctgttctatg tgtcctggct gcagcatcag cccagaaact ctagagccag aggacccaga      120

agggcctctg ctgctggacc tagagtgaca gtgcttgtca gagagtttga ggcctttgac      180

aatgctgtgc ctgagctggt ggacagcttc ctgcagcaag atcctgctca gcctgtggtg      240

gtggctgctg atacactgcc ttatcctcca ctggctctgc ctagaatccc caatgttaga      300

ctggccctcc tgcagcctgc tctggataga cctgctgctg cttccagacc tgagacatat      360

gtggccactg agtttgtggc cctggtgcct gatggtgcca gagctgaagc tcctggcctg      420

ctggaaagaa tggttgaggc cctgagagct ggatctgcca gactggttgc tgctcctgtg      480

gctacagcca atcctgccag atgtctggcc ctgaatgtgt ccctgagaga atggacagcc      540

agatatggtg ctgccccagc tgctcctaga tgtgatgctc ttgatgggga tgctgtggtc      600

ctgctgagag ccagggatct gttcaatctg tctgccccac tggccagacc tgtgggcaca      660

tctctgtttc tgcagacagc tctgagaggc tgggctgtgc agctgctgga tctcaccttt      720

gctgctgcaa gacagcctcc tctggccaca gctcatgcca gatggaaggc tgagagagag      780

ggcagagcta gaagggctgc tctgctcaga gcactgggca tcagactggt gtcttgggaa      840

ggtggcagac ttgagtggtt tggctgcaac aaagaaacca ccagatgctt tggcacagtt      900

gtgggagaca cccctgccta cctgtatgag gaaagatgga ccccaccttg ctgtctgaga      960

gccctgaggg aaacagctag atatgttgtt ggagtgcttg aggctgctgg tgtcagatac     1020

tggctggaag gtggaagtct gctgggagct gctaggcatg gggacatcat cccttgggac     1080

tatgatgtgg acctgggcat ctacctggaa gatgtgggca attgtgaaca gctgagaggg     1140

gctgaagctg gctctgttgt ggatgagaga ggctttgtct gggagaaagc tgttgaggga     1200

gacttcttca gagtgcagta ctctgagagc aaccacctcc atgtggatct gtggccattc     1260

taccccagaa atggggtcat gacaaaggac acctggctgg accacagaca ggatgtggaa     1320

ttccctgagc actttctgca gcctctggtg ccactgcctt ttgctggatt tgtggctcag     1380

gcccctaaca actacagaag attcctggaa ctgaagtttg gccctggggt catagagaac     1440

cctcagtacc ctaatcctgc actgctgagc ctgactggat ctggctgatg a              1491


<210>  2
<211>  400
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic promoter (Syn100)

<400>  2
cggccgtccg ccctcggcac cattcctcac gacaccgaaa tatggcgacg ggtgaggaat       60

ggtggggagt tatttttaga gcggtgagga atggtgggca ggcagcaggt gttgggggag      120

ttatttttag agcggggagt tatttttaga gcggtgagga atggtggaca ccgaaatatg      180

gcgacgggtg aggaatggtg ccgtcgccat atttgggtgt cccgtccgcc ctcggccggg      240

gccgcattcc tgggggccgg gcggtgctcc cgcccgcctc gataaaaggc tccggggccg      300

gcggcggccc acgagctacc cggaggagcg ggaggcgtct ctgccagcgg tccgacgcgc      360

agtcagcacc aggtaggtgg gcaccgcgcc gtgccgtgcc                            400


<210>  3
<211>  82
<212>  DNA
<213>  Homo sapiens

<400>  3
gtgagtatct cagggatcca gacatgggga tatgggaggt gcctctgatc ccagggctca       60

ctgtgggtct ctctgttcac ag                                                82


<210>  4
<211>  232
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  poly A

<400>  4
tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc       60

ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa      120

tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg      180

gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg ag              232


<210>  5
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  poly A

<400>  5
aggcctaata aagagctcag atgcatcgat cagagtgtgt tggttttttg tgtg             54


<210>  6
<211>  1545
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  opti-hu-FKRP-CpG(-) with Poly A tail

<400>  6
atgagactga caagatgtca agctgccctg gctgctgcca tcacactgaa tctgctggtg       60

ctgttctatg tgtcctggct gcagcatcag cccagaaact ctagagccag aggacccaga      120

agggcctctg ctgctggacc tagagtgaca gtgcttgtca gagagtttga ggcctttgac      180

aatgctgtgc ctgagctggt ggacagcttc ctgcagcaag atcctgctca gcctgtggtg      240

gtggctgctg atacactgcc ttatcctcca ctggctctgc ctagaatccc caatgttaga      300

ctggccctcc tgcagcctgc tctggataga cctgctgctg cttccagacc tgagacatat      360

gtggccactg agtttgtggc cctggtgcct gatggtgcca gagctgaagc tcctggcctg      420

ctggaaagaa tggttgaggc cctgagagct ggatctgcca gactggttgc tgctcctgtg      480

gctacagcca atcctgccag atgtctggcc ctgaatgtgt ccctgagaga atggacagcc      540

agatatggtg ctgccccagc tgctcctaga tgtgatgctc ttgatgggga tgctgtggtc      600

ctgctgagag ccagggatct gttcaatctg tctgccccac tggccagacc tgtgggcaca      660

tctctgtttc tgcagacagc tctgagaggc tgggctgtgc agctgctgga tctcaccttt      720

gctgctgcaa gacagcctcc tctggccaca gctcatgcca gatggaaggc tgagagagag      780

ggcagagcta gaagggctgc tctgctcaga gcactgggca tcagactggt gtcttgggaa      840

ggtggcagac ttgagtggtt tggctgcaac aaagaaacca ccagatgctt tggcacagtt      900

gtgggagaca cccctgccta cctgtatgag gaaagatgga ccccaccttg ctgtctgaga      960

gccctgaggg aaacagctag atatgttgtt ggagtgcttg aggctgctgg tgtcagatac     1020

tggctggaag gtggaagtct gctgggagct gctaggcatg gggacatcat cccttgggac     1080

tatgatgtgg acctgggcat ctacctggaa gatgtgggca attgtgaaca gctgagaggg     1140

gctgaagctg gctctgttgt ggatgagaga ggctttgtct gggagaaagc tgttgaggga     1200

gacttcttca gagtgcagta ctctgagagc aaccacctcc atgtggatct gtggccattc     1260

taccccagaa atggggtcat gacaaaggac acctggctgg accacagaca ggatgtggaa     1320

ttccctgagc actttctgca gcctctggtg ccactgcctt ttgctggatt tgtggctcag     1380

gcccctaaca actacagaag attcctggaa ctgaagtttg gccctggggt catagagaac     1440

cctcagtacc ctaatcctgc actgctgagc ctgactggat ctggctgatg aaggcctaat     1500

aaagagctca gatgcatcga tcagagtgtg ttggtttttt gtgtg                     1545


<210>  7
<211>  2027
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  opti-hu-FKRP expression construct

<400>  7
cggccgtccg ccctcggcac cattcctcac gacaccgaaa tatggcgacg ggtgaggaat       60

ggtggggagt tatttttaga gcggtgagga atggtgggca ggcagcaggt gttgggggag      120

ttatttttag agcggggagt tatttttaga gcggtgagga atggtggaca ccgaaatatg      180

gcgacgggtg aggaatggtg ccgtcgccat atttgggtgt cccgtccgcc ctcggccggg      240

gccgcattcc tgggggccgg gcggtgctcc cgcccgcctc gataaaaggc tccggggccg      300

gcggcggccc acgagctacc cggaggagcg ggaggcgtct ctgccagcgg tccgacgcgc      360

agtcagcacc aggtaggtgg gcaccgcgcc gtgccgtgcc gtgagtatct cagggatcca      420

gacatgggga tatgggaggt gcctctgatc ccagggctca ctgtgggtct ctctgttcac      480

agatgagact gacaagatgt caagctgccc tggctgctgc catcacactg aatctgctgg      540

tgctgttcta tgtgtcctgg ctgcagcatc agcccagaaa ctctagagcc agaggaccca      600

gaagggcctc tgctgctgga cctagagtga cagtgcttgt cagagagttt gaggcctttg      660

acaatgctgt gcctgagctg gtggacagct tcctgcagca agatcctgct cagcctgtgg      720

tggtggctgc tgatacactg ccttatcctc cactggctct gcctagaatc cccaatgtta      780

gactggccct cctgcagcct gctctggata gacctgctgc tgcttccaga cctgagacat      840

atgtggccac tgagtttgtg gccctggtgc ctgatggtgc cagagctgaa gctcctggcc      900

tgctggaaag aatggttgag gccctgagag ctggatctgc cagactggtt gctgctcctg      960

tggctacagc caatcctgcc agatgtctgg ccctgaatgt gtccctgaga gaatggacag     1020

ccagatatgg tgctgcccca gctgctccta gatgtgatgc tcttgatggg gatgctgtgg     1080

tcctgctgag agccagggat ctgttcaatc tgtctgcccc actggccaga cctgtgggca     1140

catctctgtt tctgcagaca gctctgagag gctgggctgt gcagctgctg gatctcacct     1200

ttgctgctgc aagacagcct cctctggcca cagctcatgc cagatggaag gctgagagag     1260

agggcagagc tagaagggct gctctgctca gagcactggg catcagactg gtgtcttggg     1320

aaggtggcag acttgagtgg tttggctgca acaaagaaac caccagatgc tttggcacag     1380

ttgtgggaga cacccctgcc tacctgtatg aggaaagatg gaccccacct tgctgtctga     1440

gagccctgag ggaaacagct agatatgttg ttggagtgct tgaggctgct ggtgtcagat     1500

actggctgga aggtggaagt ctgctgggag ctgctaggca tggggacatc atcccttggg     1560

actatgatgt ggacctgggc atctacctgg aagatgtggg caattgtgaa cagctgagag     1620

gggctgaagc tggctctgtt gtggatgaga gaggctttgt ctgggagaaa gctgttgagg     1680

gagacttctt cagagtgcag tactctgaga gcaaccacct ccatgtggat ctgtggccat     1740

tctaccccag aaatggggtc atgacaaagg acacctggct ggaccacaga caggatgtgg     1800

aattccctga gcactttctg cagcctctgg tgccactgcc ttttgctgga tttgtggctc     1860

aggcccctaa caactacaga agattcctgg aactgaagtt tggccctggg gtcatagaga     1920

accctcagta ccctaatcct gcactgctga gcctgactgg atctggctga tgaaggccta     1980

ataaagagct cagatgcatc gatcagagtg tgttggtttt ttgtgtg                   2027


<210>  8
<211>  1491
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  opti-huFKRP

<400>  8
atgagactga caagatgcca ggccgccctg gccgctgcca tcacactgaa tctgctggtg       60

ctgttctatg tgtcctggct gcagcaccag ccccggaact ctagagccag aggcccaaga      120

agggcctctg ccgccggacc tagagtgaca gtgctcgtgc gcgagttcga ggccttcgac      180

aatgccgtgc ccgagctggt ggacagcttc ctgcagcaag accctgctca gcctgtggtg      240

gtggccgccg atacactgcc ttatcctcca ctggccctgc ccagaatccc caatgtgcga      300

ctggctctgc tgcagcccgc cctggataga cctgccgccg ctagcagacc tgagacatac      360

gtggccaccg agttcgtggc cctggtgcct gatggcgcca gagctgaagc tcccggcctg      420

ctggaaagaa tggtggaagc cctgagagcc ggcagcgcca gactggtggc tgctcctgtg      480

gctaccgcca accctgccag atgtctggcc ctgaatgtgt ccctgagaga gtggaccgcc      540

agatacggcg ctgcccctgc cgctcctaga tgtgatgctc tggatggcga cgccgtggtg      600

ctgctgagag ccagggacct gttcaacctg agcgcccctc tggccagacc tgtgggcaca      660

agcctgtttc tgcagacagc cctgaggggc tgggccgtgc agctgctgga tctgacattt      720

gccgctgcca gacagcctcc tctggccaca gcccatgcca gatggaaggc cgagagagag      780

ggcagagcca gaagggctgc tctgctgagg gccctgggca tcagactggt gtcttgggaa      840

ggcggcagac tcgagtggtt cggctgcaac aaagaaacca cccggtgctt cggcaccgtc      900

gtgggcgata caccagccta cctgtacgag gaaagatgga cccccccttg ctgcctgcgg      960

gccctgagag aaacagccag atatgtcgtg ggcgtgctgg aagccgctgg cgtgcgatat     1020

tggctggaag gcggatctct gctgggagcc gccaggcacg gcgacatcat cccttgggac     1080

tacgacgtgg acctgggcat ctacctggaa gatgtgggca actgcgagca gctgagaggc     1140

gccgaagccg gctctgtggt ggatgagagg ggcttcgtgt gggagaaggc cgtggaaggc     1200

gacttcttcc gggtgcagta cagcgagagc aaccatctgc atgtggacct gtggcccttc     1260

tacccccgga acggcgtgat gaccaaggac acctggctgg accaccggca ggacgtggaa     1320

ttccccgagc actttctgca gcccctggtg ccactgcctt tcgccggatt tgtggcccag     1380

gcccccaaca actaccggcg gttcctggaa ctgaagttcg gccctggcgt gatcgagaac     1440

ccccagtacc ctaaccctgc cctgctgagc ctgaccggca gcggctaatg a              1491


<210>  9
<211>  100
<212>  DNA
<213>  Gallus gallus

<400>  9
gcggcggccc acgagctacc cggaggagcg ggaggcgtct ctgccagcgg tccgacgcgc       60

agtcagcacc aggtaggtgg gcaccgcgcc gtgccgtgcc                            100


<210>  10
<211>  1488
<212>  DNA
<213>  Homo sapiens

<400>  10
atgcggctca cccgctgcca ggctgccctg gcggccgcca tcaccctcaa ccttctggtc       60

ctcttctatg tctcgtggct gcagcaccag cctaggaatt cccgggcccg ggggccccgt      120

cgtgcctctg ctgccggccc ccgtgtcacc gtcctggtgc gggagttcga ggcatttgac      180

aacgcggtgc ccgagctggt agactccttc ctgcagcaag acccagccca gcccgtggtg      240

gtggcagccg acacgctccc ctacccgccc ctggccctgc cccgcatccc caacgtgcgt      300

ctggcgctgc tccagcccgc cctggaccgg ccagccgcag cctcgcgccc ggagacctac      360

gtggccaccg agtttgtggc cctagtacct gatggggcgc gggctgaggc acctggcctg      420

ctggagcgca tggtggaggc gctccgcgca ggaagcgcac gtctggtggc cgccccggtt      480

gccacggcca accctgccag gtgcctggcc ctgaacgtca gcctgcgaga gtggaccgcc      540

cgctatggcg cagcccccgc cgcgccccgc tgcgacgccc tggacggaga tgctgtggtg      600

ctcctgcgcg cccgcgacct cttcaacctc tcggcgcccc tggcccggcc ggtgggcacc      660

agcctctttc tgcagaccgc ccttcgcggc tgggcggtgc agctgctgga cttgaccttc      720

gccgcggcgc gccagccccc gctggccacg gcccacgcgc gctggaaggc tgagcgcgag      780

ggacgcgctc ggcgggcggc gctgctccgc gcgctgggca tccgcctagt gagctgggaa      840

ggcgggcggc tggagtggtt cggctgcaac aaggagacca cgcgctgctt cggaaccgtg      900

gtgggcgaca cgcccgccta cctctacgag gagcgctgga cgcccccctg ctgcctgcgc      960

gcgctgcgcg agaccgcccg ctatgtggtg ggcgtgctgg aggctgcggg cgtgcgctac     1020

tggctcgagg gcggctcact gctgggggcc gcccgccacg gggacatcat cccatgggac     1080

tacgacgtgg acctgggcat ctacttggag gacgtgggca actgcgagca gctgcggggg     1140

gcagaggccg gctcggtggt ggatgagcgc ggcttcgtat gggagaaggc ggtcgagggc     1200

gactttttcc gcgtgcagta cagcgaaagc aaccacttgc acgtggacct gtggcccttc     1260

tacccccgca atggcgtcat gaccaaggac acgtggctgg accaccggca ggatgtggag     1320

tttcccgagc acttcctgca gccgctggtg cccctgccct ttgccggctt cgtggcgcag     1380

gcgcctaaca actaccgccg cttcctggag ctcaagttcg ggcccggggt catcgagaac     1440

ccccagtacc ccaacccggc actgctgagt ctgacgggaa gcggctga                  1488


