                         SEQUENCE LISTING

<110>  Seattle Children's Hospital d/b/a Seattle Children's 
       Research Institute
 
<120>  VASCULAR-ENDOTHELIAL CADHERIN ACTIVATING ANTIBODIES AND USES 
       THEREOF

<130>  SCRI.350WO/S281-0023PCT

<150>  US 63/132,666
<151>  2020-12-31

<160>  68    

<170>  PatentIn version 3.5

<210>  1
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthesized CDRH1 of 8A12c antibody

<400>  1

Ser Tyr Asn Met His 
1               5   


<210>  2
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthesized CDRH2 of 8A12c antibody

<400>  2

Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  3
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthesized CDRH3 of 8A12c antibody

<400>  3

Glu Tyr Tyr Tyr Gly Ser Ser Phe Asp Phe 
1               5                   10  


<210>  4
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthesized CDRL1 of 8A12c antibody

<400>  4

Arg Ser Ser Gln Ser Ile Val His Ser Asn Gly Asn Thr Tyr Leu Glu 
1               5                   10                  15      


<210>  5
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthesized CDRL2 of 8A12c antibody

<400>  5

Lys Val Ser Asn Arg Phe Ser 
1               5           


<210>  6
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthesized CDRL3 of 8A12c antibody

<400>  6

Phe Gln Gly Ser His Val Pro Trp Thr 
1               5                   


<210>  7
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthesized CDRH1 of 3A5a antibody

<400>  7

Ser Tyr Trp Met Gln 
1               5   


<210>  8
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthesized CDRH2 of 3A5a antibody

<400>  8

Glu Ile Asp Pro Ser Asp Ser Tyr Thr Asn Tyr Asn Gln Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  9
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthesized CDRH3 of 3A5a antibody

<400>  9

Gly Gly Leu Asp Tyr 
1               5   


<210>  10
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthesized CDRL1 of 3A5a antibody

<400>  10

Arg Ser Ser Gln Ser Ile Val His Ser Asn Gly Asn Thr Tyr Leu Tyr 
1               5                   10                  15      


<210>  11
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthesized CDRL2 of 3A5a antibody

<400>  11

Arg Val Ser Asn Arg Phe Ser 
1               5           


<210>  12
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthesized CDRL3 of 3A5a antibody

<400>  12

Phe Gln Gly Thr His Val Pro Leu Thr 
1               5                   


<210>  13
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthesized heavy chain variable (VH) domain of 8A12c antibody 
       without leader peptide

<400>  13

Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Asn Met His Trp Val Lys Gln Thr Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Glu Tyr Tyr Tyr Gly Ser Ser Phe Asp Phe Trp Gly Gln Gly 
            100                 105                 110         


Thr Thr Leu Thr Val Ser Ser 
        115                 


<210>  14
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthesized light chain variable (VL) domain of 8A12c antibody 
       without leader peptide

<400>  14

Asp Val Leu Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu Glu Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr Cys Phe Gln Gly 
                85                  90                  95      


Ser His Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  15
<211>  138
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthesized heavy chain variable (VH) domain of 8A12c antibody 
       with leader peptide

<400>  15

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Ser Tyr Asn Met His Trp Val Lys Gln Thr Pro Gly Gln Gly Leu 
    50                  55                  60                  


Glu Trp Ile Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Glu Tyr Tyr Tyr Gly Ser Ser Phe Asp Phe Trp 
        115                 120                 125             


Gly Gln Gly Thr Thr Leu Thr Val Ser Ser 
    130                 135             


<210>  16
<211>  131
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthesized light chain variable (VL) domain of 8A12c antibody 
       with leader peptide

<400>  16

Met Lys Leu Pro Val Arg Leu Leu Val Leu Met Phe Trp Ile Pro Ala 
1               5                   10                  15      


Ser Ser Ser Asp Val Leu Met Thr Gln Thr Pro Leu Ser Leu Pro Val 
            20                  25                  30          


Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile 
        35                  40                  45              


Val His Ser Asn Gly Asn Thr Tyr Leu Glu Trp Tyr Leu Gln Lys Pro 
    50                  55                  60                  


Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser 
65                  70                  75                  80  


Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr 
                85                  90                  95      


Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr Cys 
            100                 105                 110         


Phe Gln Gly Ser His Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu 
        115                 120                 125             


Glu Ile Lys 
    130     


<210>  17
<211>  443
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthesized heavy chain of 8A12c antibody without leader peptide

<400>  17

Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Asn Met His Trp Val Lys Gln Thr Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Glu Tyr Tyr Tyr Gly Ser Ser Phe Asp Phe Trp Gly Gln Gly 
            100                 105                 110         


Thr Thr Leu Thr Val Ser Ser Ala Lys Thr Thr Pro Pro Ser Val Tyr 
        115                 120                 125             


Pro Leu Ala Pro Gly Ser Ala Ala Gln Thr Asn Ser Met Val Thr Leu 
    130                 135                 140                 


Gly Cys Leu Val Lys Gly Tyr Phe Pro Glu Pro Val Thr Val Thr Trp 
145                 150                 155                 160 


Asn Ser Gly Ser Leu Ser Ser Gly Val His Thr Phe Pro Ala Val Leu 
                165                 170                 175     


Gln Ser Asp Leu Tyr Thr Leu Ser Ser Ser Val Thr Val Pro Ser Ser 
            180                 185                 190         


Pro Arg Pro Ser Glu Thr Val Thr Cys Asn Val Ala His Pro Ala Ser 
        195                 200                 205             


Ser Thr Lys Val Asp Lys Lys Ile Val Pro Arg Asp Cys Gly Cys Lys 
    210                 215                 220                 


Pro Cys Ile Cys Thr Val Pro Glu Val Ser Ser Val Phe Ile Phe Pro 
225                 230                 235                 240 


Pro Lys Pro Lys Asp Val Leu Thr Ile Thr Leu Thr Pro Lys Val Thr 
                245                 250                 255     


Cys Val Val Val Asp Ile Ser Lys Asp Asp Pro Glu Val Gln Phe Ser 
            260                 265                 270         


Trp Phe Val Asp Asp Val Glu Val His Thr Ala Gln Thr Gln Pro Arg 
        275                 280                 285             


Glu Glu Gln Phe Asn Ser Thr Phe Arg Ser Val Ser Glu Leu Pro Ile 
    290                 295                 300                 


Met His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys Cys Arg Val Asn 
305                 310                 315                 320 


Ser Ala Ala Phe Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys 
                325                 330                 335     


Gly Arg Pro Lys Ala Pro Gln Val Tyr Thr Ile Pro Pro Pro Lys Glu 
            340                 345                 350         


Gln Met Ala Lys Asp Lys Val Ser Leu Thr Cys Met Ile Thr Asp Phe 
        355                 360                 365             


Phe Pro Glu Asp Ile Thr Val Glu Trp Gln Trp Asn Gly Gln Pro Ala 
    370                 375                 380                 


Glu Asn Tyr Lys Asn Thr Gln Pro Ile Met Asn Thr Asn Gly Ser Tyr 
385                 390                 395                 400 


Phe Val Tyr Ser Lys Leu Asn Val Gln Lys Ser Asn Trp Glu Ala Gly 
                405                 410                 415     


Asn Thr Phe Thr Cys Ser Val Leu His Glu Gly Leu His Asn His His 
            420                 425                 430         


Thr Glu Lys Ser Leu Ser His Ser Pro Gly Lys 
        435                 440             


<210>  18
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthesized light chain of 8A12c antibody without leader peptide

<400>  18

Asp Val Leu Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu Glu Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr Cys Phe Gln Gly 
                85                  90                  95      


Ser His Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro Ser Ser Glu 
        115                 120                 125             


Gln Leu Thr Ser Gly Gly Ala Ser Val Val Cys Phe Leu Asn Asn Phe 
    130                 135                 140                 


Tyr Pro Lys Asp Ile Asn Val Lys Trp Lys Ile Asp Gly Ser Glu Arg 
145                 150                 155                 160 


Gln Asn Gly Val Leu Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp Ser 
                165                 170                 175     


Thr Tyr Ser Met Ser Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr Glu 
            180                 185                 190         


Arg His Asn Ser Tyr Thr Cys Glu Ala Thr His Lys Thr Ser Thr Ser 
        195                 200                 205             


Pro Ile Val Lys Ser Phe Asn Arg Asn Glu Cys 
    210                 215                 


<210>  19
<211>  462
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthesized heavy chain of 8A12c antibody with leader peptide

<400>  19

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Ser Tyr Asn Met His Trp Val Lys Gln Thr Pro Gly Gln Gly Leu 
    50                  55                  60                  


Glu Trp Ile Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Glu Tyr Tyr Tyr Gly Ser Ser Phe Asp Phe Trp 
        115                 120                 125             


Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Ala Lys Thr Thr Pro Pro 
    130                 135                 140                 


Ser Val Tyr Pro Leu Ala Pro Gly Ser Ala Ala Gln Thr Asn Ser Met 
145                 150                 155                 160 


Val Thr Leu Gly Cys Leu Val Lys Gly Tyr Phe Pro Glu Pro Val Thr 
                165                 170                 175     


Val Thr Trp Asn Ser Gly Ser Leu Ser Ser Gly Val His Thr Phe Pro 
            180                 185                 190         


Ala Val Leu Gln Ser Asp Leu Tyr Thr Leu Ser Ser Ser Val Thr Val 
        195                 200                 205             


Pro Ser Ser Pro Arg Pro Ser Glu Thr Val Thr Cys Asn Val Ala His 
    210                 215                 220                 


Pro Ala Ser Ser Thr Lys Val Asp Lys Lys Ile Val Pro Arg Asp Cys 
225                 230                 235                 240 


Gly Cys Lys Pro Cys Ile Cys Thr Val Pro Glu Val Ser Ser Val Phe 
                245                 250                 255     


Ile Phe Pro Pro Lys Pro Lys Asp Val Leu Thr Ile Thr Leu Thr Pro 
            260                 265                 270         


Lys Val Thr Cys Val Val Val Asp Ile Ser Lys Asp Asp Pro Glu Val 
        275                 280                 285             


Gln Phe Ser Trp Phe Val Asp Asp Val Glu Val His Thr Ala Gln Thr 
    290                 295                 300                 


Gln Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Ser Val Ser Glu 
305                 310                 315                 320 


Leu Pro Ile Met His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys Cys 
                325                 330                 335     


Arg Val Asn Ser Ala Ala Phe Pro Ala Pro Ile Glu Lys Thr Ile Ser 
            340                 345                 350         


Lys Thr Lys Gly Arg Pro Lys Ala Pro Gln Val Tyr Thr Ile Pro Pro 
        355                 360                 365             


Pro Lys Glu Gln Met Ala Lys Asp Lys Val Ser Leu Thr Cys Met Ile 
    370                 375                 380                 


Thr Asp Phe Phe Pro Glu Asp Ile Thr Val Glu Trp Gln Trp Asn Gly 
385                 390                 395                 400 


Gln Pro Ala Glu Asn Tyr Lys Asn Thr Gln Pro Ile Met Asn Thr Asn 
                405                 410                 415     


Gly Ser Tyr Phe Val Tyr Ser Lys Leu Asn Val Gln Lys Ser Asn Trp 
            420                 425                 430         


Glu Ala Gly Asn Thr Phe Thr Cys Ser Val Leu His Glu Gly Leu His 
        435                 440                 445             


Asn His His Thr Glu Lys Ser Leu Ser His Ser Pro Gly Lys 
    450                 455                 460         


<210>  20
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthesized light chain of 8A12c antibody with leader peptide

<400>  20

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Asp Val Leu Met Thr Gln Thr Pro Leu Ser Leu Pro Val 
            20                  25                  30          


Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile 
        35                  40                  45              


Val His Ser Asn Gly Asn Thr Tyr Leu Glu Trp Tyr Leu Gln Lys Pro 
    50                  55                  60                  


Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser 
65                  70                  75                  80  


Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr 
                85                  90                  95      


Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr Cys 
            100                 105                 110         


Phe Gln Gly Ser His Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu 
        115                 120                 125             


Glu Ile Lys Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro 
    130                 135                 140                 


Ser Ser Glu Gln Leu Thr Ser Gly Gly Ala Ser Val Val Cys Phe Leu 
145                 150                 155                 160 


Asn Asn Phe Tyr Pro Lys Asp Ile Asn Val Lys Trp Lys Ile Asp Gly 
                165                 170                 175     


Ser Glu Arg Gln Asn Gly Val Leu Asn Ser Trp Thr Asp Gln Asp Ser 
            180                 185                 190         


Lys Asp Ser Thr Tyr Ser Met Ser Ser Thr Leu Thr Leu Thr Lys Asp 
        195                 200                 205             


Glu Tyr Glu Arg His Asn Ser Tyr Thr Cys Glu Ala Thr His Lys Thr 
    210                 215                 220                 


Ser Thr Ser Pro Ile Val Lys Ser Phe Asn Arg Asn Glu Cys 
225                 230                 235             


<210>  21
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthesized heavy chain variable (VH) domain of 3A5c antibody 
       without leader peptide

<400>  21

Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Trp Met Gln Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile Asp Pro Ser Asp Ser Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Thr Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly Leu Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val 
            100                 105                 110         


Ser Ser 
        


<210>  22
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthesized light chain variable (VL) domain of 3A5c antibody 
       without leader peptide

<400>  22

Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu Tyr Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Arg Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Thr Glu Asp Met Gly Ile Tyr Tyr Cys Phe Gln Gly 
                85                  90                  95      


Thr His Val Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105                 110         


<210>  23
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthesized heavy chain variable (VH) domain of 3A5c antibody 
       with leader peptide

<400>  23

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Ser Tyr Trp Met Gln Trp Val Lys Gln Arg Pro Gly Gln Gly Leu 
    50                  55                  60                  


Glu Trp Ile Gly Glu Ile Asp Pro Ser Asp Ser Tyr Thr Asn Tyr Asn 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Val Asp Thr Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Gly Gly Leu Asp Tyr Trp Gly Gln Gly Thr Thr 
        115                 120                 125             


Leu Thr Val Ser Ser 
    130             


<210>  24
<211>  131
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthesized light chain variable (VL) domain of 3A5c antibody 
       with leader peptide

<400>  24

Met Lys Leu Pro Val Arg Leu Leu Val Leu Met Phe Trp Ile Pro Ala 
1               5                   10                  15      


Ser Ser Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val 
            20                  25                  30          


Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile 
        35                  40                  45              


Val His Ser Asn Gly Asn Thr Tyr Leu Tyr Trp Tyr Leu Gln Lys Pro 
    50                  55                  60                  


Gly Gln Ser Pro Lys Leu Leu Ile Tyr Arg Val Ser Asn Arg Phe Ser 
65                  70                  75                  80  


Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr 
                85                  90                  95      


Leu Lys Ile Ser Arg Val Glu Thr Glu Asp Met Gly Ile Tyr Tyr Cys 
            100                 105                 110         


Phe Gln Gly Thr His Val Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu 
        115                 120                 125             


Glu Leu Lys 
    130     


<210>  25
<211>  438
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthesized heavy chain of 3A5c antibody without leader peptide

<400>  25

Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Trp Met Gln Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile Asp Pro Ser Asp Ser Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Thr Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly Leu Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val 
            100                 105                 110         


Ser Ser Ala Lys Thr Thr Pro Pro Ser Val Tyr Pro Leu Ala Pro Gly 
        115                 120                 125             


Ser Ala Ala Gln Thr Asn Ser Met Val Thr Leu Gly Cys Leu Val Lys 
    130                 135                 140                 


Gly Tyr Phe Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ser Leu 
145                 150                 155                 160 


Ser Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Asp Leu Tyr 
                165                 170                 175     


Thr Leu Ser Ser Ser Val Thr Val Pro Ser Ser Pro Arg Pro Ser Glu 
            180                 185                 190         


Thr Val Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp 
        195                 200                 205             


Lys Lys Ile Val Pro Arg Asp Cys Gly Cys Lys Pro Cys Ile Cys Thr 
    210                 215                 220                 


Val Pro Glu Val Ser Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp 
225                 230                 235                 240 


Val Leu Thr Ile Thr Leu Thr Pro Lys Val Thr Cys Val Val Val Asp 
                245                 250                 255     


Ile Ser Lys Asp Asp Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp 
            260                 265                 270         


Val Glu Val His Thr Ala Gln Thr Gln Pro Arg Glu Glu Gln Phe Asn 
        275                 280                 285             


Ser Thr Phe Arg Ser Val Ser Glu Leu Pro Ile Met His Gln Asp Trp 
    290                 295                 300                 


Leu Asn Gly Lys Glu Phe Lys Cys Arg Val Asn Ser Ala Ala Phe Pro 
305                 310                 315                 320 


Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Arg Pro Lys Ala 
                325                 330                 335     


Pro Gln Val Tyr Thr Ile Pro Pro Pro Lys Glu Gln Met Ala Lys Asp 
            340                 345                 350         


Lys Val Ser Leu Thr Cys Met Ile Thr Asp Phe Phe Pro Glu Asp Ile 
        355                 360                 365             


Thr Val Glu Trp Gln Trp Asn Gly Gln Pro Ala Glu Asn Tyr Lys Asn 
    370                 375                 380                 


Thr Gln Pro Ile Met Asn Thr Asn Gly Ser Tyr Phe Val Tyr Ser Lys 
385                 390                 395                 400 


Leu Asn Val Gln Lys Ser Asn Trp Glu Ala Gly Asn Thr Phe Thr Cys 
                405                 410                 415     


Ser Val Leu His Glu Gly Leu His Asn His His Thr Glu Lys Ser Leu 
            420                 425                 430         


Ser His Ser Pro Gly Lys 
        435             


<210>  26
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthesized light chain of 3A5c antibody without leader peptide

<400>  26

Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu Tyr Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Arg Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Thr Glu Asp Met Gly Ile Tyr Tyr Cys Phe Gln Gly 
                85                  90                  95      


Thr His Val Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105                 110         


Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro Ser Ser Glu 
        115                 120                 125             


Gln Leu Thr Ser Gly Gly Ala Ser Val Val Cys Phe Leu Asn Asn Phe 
    130                 135                 140                 


Tyr Pro Lys Asp Ile Asn Val Lys Trp Lys Ile Asp Gly Ser Glu Arg 
145                 150                 155                 160 


Gln Asn Gly Val Leu Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp Ser 
                165                 170                 175     


Thr Tyr Ser Met Ser Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr Glu 
            180                 185                 190         


Arg His Asn Ser Tyr Thr Cys Glu Ala Thr His Lys Thr Ser Thr Ser 
        195                 200                 205             


Pro Ile Val Lys Ser Phe Asn Arg Asn Glu Cys 
    210                 215                 


<210>  27
<211>  457
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthesized heavy chain of 3A5c antibody with leader peptide

<400>  27

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Ser Tyr Trp Met Gln Trp Val Lys Gln Arg Pro Gly Gln Gly Leu 
    50                  55                  60                  


Glu Trp Ile Gly Glu Ile Asp Pro Ser Asp Ser Tyr Thr Asn Tyr Asn 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Val Asp Thr Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Gly Gly Leu Asp Tyr Trp Gly Gln Gly Thr Thr 
        115                 120                 125             


Leu Thr Val Ser Ser Ala Lys Thr Thr Pro Pro Ser Val Tyr Pro Leu 
    130                 135                 140                 


Ala Pro Gly Ser Ala Ala Gln Thr Asn Ser Met Val Thr Leu Gly Cys 
145                 150                 155                 160 


Leu Val Lys Gly Tyr Phe Pro Glu Pro Val Thr Val Thr Trp Asn Ser 
                165                 170                 175     


Gly Ser Leu Ser Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser 
            180                 185                 190         


Asp Leu Tyr Thr Leu Ser Ser Ser Val Thr Val Pro Ser Ser Pro Arg 
        195                 200                 205             


Pro Ser Glu Thr Val Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr 
    210                 215                 220                 


Lys Val Asp Lys Lys Ile Val Pro Arg Asp Cys Gly Cys Lys Pro Cys 
225                 230                 235                 240 


Ile Cys Thr Val Pro Glu Val Ser Ser Val Phe Ile Phe Pro Pro Lys 
                245                 250                 255     


Pro Lys Asp Val Leu Thr Ile Thr Leu Thr Pro Lys Val Thr Cys Val 
            260                 265                 270         


Val Val Asp Ile Ser Lys Asp Asp Pro Glu Val Gln Phe Ser Trp Phe 
        275                 280                 285             


Val Asp Asp Val Glu Val His Thr Ala Gln Thr Gln Pro Arg Glu Glu 
    290                 295                 300                 


Gln Phe Asn Ser Thr Phe Arg Ser Val Ser Glu Leu Pro Ile Met His 
305                 310                 315                 320 


Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys Cys Arg Val Asn Ser Ala 
                325                 330                 335     


Ala Phe Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Arg 
            340                 345                 350         


Pro Lys Ala Pro Gln Val Tyr Thr Ile Pro Pro Pro Lys Glu Gln Met 
        355                 360                 365             


Ala Lys Asp Lys Val Ser Leu Thr Cys Met Ile Thr Asp Phe Phe Pro 
    370                 375                 380                 


Glu Asp Ile Thr Val Glu Trp Gln Trp Asn Gly Gln Pro Ala Glu Asn 
385                 390                 395                 400 


Tyr Lys Asn Thr Gln Pro Ile Met Asn Thr Asn Gly Ser Tyr Phe Val 
                405                 410                 415     


Tyr Ser Lys Leu Asn Val Gln Lys Ser Asn Trp Glu Ala Gly Asn Thr 
            420                 425                 430         


Phe Thr Cys Ser Val Leu His Glu Gly Leu His Asn His His Thr Glu 
        435                 440                 445             


Lys Ser Leu Ser His Ser Pro Gly Lys 
    450                 455         


<210>  28
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthesized light chain of 3A5c antibody with leader peptide

<400>  28

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val 
            20                  25                  30          


Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile 
        35                  40                  45              


Val His Ser Asn Gly Asn Thr Tyr Leu Tyr Trp Tyr Leu Gln Lys Pro 
    50                  55                  60                  


Gly Gln Ser Pro Lys Leu Leu Ile Tyr Arg Val Ser Asn Arg Phe Ser 
65                  70                  75                  80  


Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr 
                85                  90                  95      


Leu Lys Ile Ser Arg Val Glu Thr Glu Asp Met Gly Ile Tyr Tyr Cys 
            100                 105                 110         


Phe Gln Gly Thr His Val Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu 
        115                 120                 125             


Glu Leu Lys Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro 
    130                 135                 140                 


Ser Ser Glu Gln Leu Thr Ser Gly Gly Ala Ser Val Val Cys Phe Leu 
145                 150                 155                 160 


Asn Asn Phe Tyr Pro Lys Asp Ile Asn Val Lys Trp Lys Ile Asp Gly 
                165                 170                 175     


Ser Glu Arg Gln Asn Gly Val Leu Asn Ser Trp Thr Asp Gln Asp Ser 
            180                 185                 190         


Lys Asp Ser Thr Tyr Ser Met Ser Ser Thr Leu Thr Leu Thr Lys Asp 
        195                 200                 205             


Glu Tyr Glu Arg His Asn Ser Tyr Thr Cys Glu Ala Thr His Lys Thr 
    210                 215                 220                 


Ser Thr Ser Pro Ile Val Lys Ser Phe Asn Arg Asn Glu Cys 
225                 230                 235             


<210>  29
<211>  357
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized nucleotide sequence encoding VH domain of 8A12c 
       antibody without leader peptide

<400>  29
caggtgcaac tgcagcagcc tggggctgag ctggtgaagc ctggggcctc agtgaagatg       60

tcctgcaagg cttctggcta cacatttacc agttacaata tgcactgggt aaagcagaca      120

cctggacagg gcctggaatg gattggagct atttatccag gaaatggtga tacttcctac      180

aatcagaagt tcaagggcaa ggccacattg actgcagaca aatcctccag cacagcctac      240

atgcagctca gcagcctgac atctgaggac tctgcggtct attactgtgc aagagagtat      300

tactacggta gttcctttga cttctggggc caaggcacca ctctcacagt ctcctca         357


<210>  30
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized nucleotide sequence encoding VL domain of 8A12c 
       antibody without leader peptide

<400>  30
gatgttttga tgacccaaac tccactctcc ctgcctgtca gtcttggaga tcaagcctcc       60

atctcttgca gatctagtca gagcattgta catagtaatg gaaacaccta tttagaatgg      120

tacctgcaga aaccaggcca gtctccaaag ctcctgatct acaaagtttc caaccgattt      180

tctggggtcc cagacaggtt cagtggcagt ggatcaggga cagatttcac actcaagatc      240

agcagagtgg aggctgagga tctgggagtt tattactgct ttcaaggttc acatgttccg      300

tggacgttcg gtggaggcac caagctggaa atcaaa                                336


<210>  31
<211>  414
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized nucleotide sequence encoding VH domain of 8A12c 
       antibody with leader peptide

<400>  31
atgggatgga gttgtatcat cctcttcttg gtagcaacag ctacaggtgt ccactcccag       60

gtgcaactgc agcagcctgg ggctgagctg gtgaagcctg gggcctcagt gaagatgtcc      120

tgcaaggctt ctggctacac atttaccagt tacaatatgc actgggtaaa gcagacacct      180

ggacagggcc tggaatggat tggagctatt tatccaggaa atggtgatac ttcctacaat      240

cagaagttca agggcaaggc cacattgact gcagacaaat cctccagcac agcctacatg      300

cagctcagca gcctgacatc tgaggactct gcggtctatt actgtgcaag agagtattac      360

tacggtagtt cctttgactt ctggggccaa ggcaccactc tcacagtctc ctca            414


<210>  32
<211>  393
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized nucleotide sequence encoding VL domain of 8A12c 
       antibody with leader peptide

<400>  32
atgaagttgc ctgttaggct gttggtgctg atgttctgga ttcctgcttc cagcagtgat       60

gttttgatga cccaaactcc actctccctg cctgtcagtc ttggagatca agcctccatc      120

tcttgcagat ctagtcagag cattgtacat agtaatggaa acacctattt agaatggtac      180

ctgcagaaac caggccagtc tccaaagctc ctgatctaca aagtttccaa ccgattttct      240

ggggtcccag acaggttcag tggcagtgga tcagggacag atttcacact caagatcagc      300

agagtggagg ctgaggatct gggagtttat tactgctttc aaggttcaca tgttccgtgg      360

acgttcggtg gaggcaccaa gctggaaatc aaa                                   393


<210>  33
<211>  1332
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized nucleotide sequence encoding heavy chain of 8A12c 
       antibody without leader peptide

<400>  33
caggtgcaac tgcagcagcc tggggctgag ctggtgaagc ctggggcctc agtgaagatg       60

tcctgcaagg cttctggcta cacatttacc agttacaata tgcactgggt aaagcagaca      120

cctggacagg gcctggaatg gattggagct atttatccag gaaatggtga tacttcctac      180

aatcagaagt tcaagggcaa ggccacattg actgcagaca aatcctccag cacagcctac      240

atgcagctca gcagcctgac atctgaggac tctgcggtct attactgtgc aagagagtat      300

tactacggta gttcctttga cttctggggc caaggcacca ctctcacagt ctcctcagcc      360

aagaccacac ctcctagcgt gtaccctctg gctcctggat ctgccgctca gaccaatagc      420

atggtcaccc tgggctgtct ggtcaagggc tactttcctg agcctgtgac cgtgacctgg      480

aacagcggat ctctgtctag cggagtgcac acattccctg ccgtgctgca gagcgatctg      540

tacacactga gcagcagcgt gacagtgcca agcagcccta gacctagcga gacagtgacc      600

tgcaacgtgg cccatcctgc cagctctacc aaggtggaca agaaaatcgt gcccagagac      660

tgcggctgca agccctgcat ctgtacagtg cctgaggtgt ccagcgtgtt cattttccca      720

cctaagccta aggacgtgct gaccatcaca ctgaccccta aagtgacctg tgtggtggtg      780

gacatcagca aggacgaccc cgaggtgcag ttcagttggt tcgtggacga cgtggaagtg      840

cacactgccc agacacagcc cagagaggaa cagttcaaca gcaccttcag aagcgtgtcc      900

gagctgccca tcatgcacca ggattggctg aacggcaaag agttcaagtg cagagtgaac      960

agcgccgcct ttcctgctcc tatcgagaaa acaatcagca agaccaaggg cagacccaag     1020

gctccccagg tgtacacaat ccctccacct aaagaacaga tggccaagga taaggtgtcc     1080

ctgacctgca tgatcaccga tttcttccca gaggacatca ccgtggaatg gcagtggaat     1140

ggacagcccg ccgagaacta caagaacacc cagcctatca tgaacaccaa cggcagctac     1200

ttcgtgtaca gcaagctgaa cgtgcagaag tccaactggg aggccggcaa cacctttacc     1260

tgtagcgtgc tgcacgaggg cctgcacaat caccacaccg agaagtccct gagccactct     1320

cctggaaagt ga                                                         1332


<210>  34
<211>  660
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized nucleotide sequence encoding light chain of 8A12c 
       antibody without leader peptide

<400>  34
gatgttttga tgacccaaac tccactctcc ctgcctgtca gtcttggaga tcaagcctcc       60

atctcttgca gatctagtca gagcattgta catagtaatg gaaacaccta tttagaatgg      120

tacctgcaga aaccaggcca gtctccaaag ctcctgatct acaaagtttc caaccgattt      180

tctggggtcc cagacaggtt cagtggcagt ggatcaggga cagatttcac actcaagatc      240

agcagagtgg aggctgagga tctgggagtt tattactgct ttcaaggttc acatgttccg      300

tggacgttcg gtggaggcac caagctggaa atcaaaagag ctgacgccgc tcctaccgtg      360

tccatctttc cacctagcag cgagcagctg acaagcggcg gagctagcgt cgtgtgcttc      420

ctgaacaact tctaccccaa ggacatcaac gtgaagtgga agatcgacgg cagcgagaga      480

cagaacggcg tgctgaatag ctggaccgac caggacagca aggactccac ctacagcatg      540

tccagcacac tgaccctgac caaggacgag tacgagcggc acaacagcta cacatgcgag      600

gccacacaca agaccagcac aagccccatc gtgaagtcct tcaaccggaa cgagtgctga      660


<210>  35
<211>  1389
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized nucleotide sequence encoding heavy chain of 8A12c 
       antibody with leader peptide

<400>  35
atgggctggt cctgcatcat tctgtttctg gtggccacag ccaccggcgt gcactctcag       60

gtgcaactgc agcagcctgg ggctgagctg gtgaagcctg gggcctcagt gaagatgtcc      120

tgcaaggctt ctggctacac atttaccagt tacaatatgc actgggtaaa gcagacacct      180

ggacagggcc tggaatggat tggagctatt tatccaggaa atggtgatac ttcctacaat      240

cagaagttca agggcaaggc cacattgact gcagacaaat cctccagcac agcctacatg      300

cagctcagca gcctgacatc tgaggactct gcggtctatt actgtgcaag agagtattac      360

tacggtagtt cctttgactt ctggggccaa ggcaccactc tcacagtctc ctcagccaag      420

accacacctc ctagcgtgta ccctctggct cctggatctg ccgctcagac caatagcatg      480

gtcaccctgg gctgtctggt caagggctac tttcctgagc ctgtgaccgt gacctggaac      540

agcggatctc tgtctagcgg agtgcacaca ttccctgccg tgctgcagag cgatctgtac      600

acactgagca gcagcgtgac agtgccaagc agccctagac ctagcgagac agtgacctgc      660

aacgtggccc atcctgccag ctctaccaag gtggacaaga aaatcgtgcc cagagactgc      720

ggctgcaagc cctgcatctg tacagtgcct gaggtgtcca gcgtgttcat tttcccacct      780

aagcctaagg acgtgctgac catcacactg acccctaaag tgacctgtgt ggtggtggac      840

atcagcaagg acgaccccga ggtgcagttc agttggttcg tggacgacgt ggaagtgcac      900

actgcccaga cacagcccag agaggaacag ttcaacagca ccttcagaag cgtgtccgag      960

ctgcccatca tgcaccagga ttggctgaac ggcaaagagt tcaagtgcag agtgaacagc     1020

gccgcctttc ctgctcctat cgagaaaaca atcagcaaga ccaagggcag acccaaggct     1080

ccccaggtgt acacaatccc tccacctaaa gaacagatgg ccaaggataa ggtgtccctg     1140

acctgcatga tcaccgattt cttcccagag gacatcaccg tggaatggca gtggaatgga     1200

cagcccgccg agaactacaa gaacacccag cctatcatga acaccaacgg cagctacttc     1260

gtgtacagca agctgaacgt gcagaagtcc aactgggagg ccggcaacac ctttacctgt     1320

agcgtgctgc acgagggcct gcacaatcac cacaccgaga agtccctgag ccactctcct     1380

ggaaagtga                                                             1389


<210>  36
<211>  717
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized nucleotide sequence encoding light chain of 8A12c 
       antibody with leader peptide

<400>  36
atgggctggt cctgcatcat tctgtttctg gtggccacag ccaccggcgt gcacagcgat       60

gttttgatga cccaaactcc actctccctg cctgtcagtc ttggagatca agcctccatc      120

tcttgcagat ctagtcagag cattgtacat agtaatggaa acacctattt agaatggtac      180

ctgcagaaac caggccagtc tccaaagctc ctgatctaca aagtttccaa ccgattttct      240

ggggtcccag acaggttcag tggcagtgga tcagggacag atttcacact caagatcagc      300

agagtggagg ctgaggatct gggagtttat tactgctttc aaggttcaca tgttccgtgg      360

acgttcggtg gaggcaccaa gctggaaatc aaaagagctg acgccgctcc taccgtgtcc      420

atctttccac ctagcagcga gcagctgaca agcggcggag ctagcgtcgt gtgcttcctg      480

aacaacttct accccaagga catcaacgtg aagtggaaga tcgacggcag cgagagacag      540

aacggcgtgc tgaatagctg gaccgaccag gacagcaagg actccaccta cagcatgtcc      600

agcacactga ccctgaccaa ggacgagtac gagcggcaca acagctacac atgcgaggcc      660

acacacaaga ccagcacaag ccccatcgtg aagtccttca accggaacga gtgctga         717


<210>  37
<211>  342
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized nucleotide sequence encoding VH domain of 3A5c 
       antibody without leader peptide

<400>  37
caggtccaac tgcagcagcc tggggctgag cttgtgaagc ctggggcttc agtgaagctg       60

tcctgcaagg cttctggcta caccttcacc agctactgga tgcagtgggt aaaacagagg      120

cctggacagg gccttgagtg gatcggagaa attgatcctt ctgatagcta tactaactac      180

aatcaaaagt tcaagggcaa ggccacattg actgtagaca catcctccag cacagcctac      240

atgcagctca gcagcctgac atctgaggac tctgcggtct attactgtgc aagaggggga      300

cttgactact ggggccaagg caccactctc acagtctcct ca                         342


<210>  38
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized nucleotide sequence encoding VL domain of3A5c 
       antibody without leader peptide

<400>  38
gatgttgtga tgacccaaac tccactctcc ctgcctgtca gtcttggaga tcaagcctcc       60

atctcttgca gatctagtca gagcattgta cacagtaatg gaaacaccta tttatattgg      120

tacctgcaga aaccaggcca gtctccaaag ctcctgatct acagggtttc caaccgattt      180

tctggggtcc cagacaggtt cagtggcagt ggatcaggga cagatttcac actcaagatc      240

agcagagtgg agactgagga tatgggaatt tattactgct ttcaaggtac acatgttccg      300

ctcacgttcg gtgctgggac caagctggag ctgaaa                                336


<210>  39
<211>  399
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized nucleotide sequence encoding VH domain of 3A5c 
       antibody with leader peptide

<400>  39
atgggatgga gctgtatcat cctcttcttg gtagcaacag ctacaggtgt ccactctcag       60

gtccaactgc agcagcctgg ggctgagctt gtgaagcctg gggcttcagt gaagctgtcc      120

tgcaaggctt ctggctacac cttcaccagc tactggatgc agtgggtaaa acagaggcct      180

ggacagggcc ttgagtggat cggagaaatt gatccttctg atagctatac taactacaat      240

caaaagttca agggcaaggc cacattgact gtagacacat cctccagcac agcctacatg      300

cagctcagca gcctgacatc tgaggactct gcggtctatt actgtgcaag agggggactt      360

gactactggg gccaaggcac cactctcaca gtctcctca                             399


<210>  40
<211>  393
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized nucleotide sequence encoding VL domain of 3A5c 
       antibody with leader peptide

<400>  40
atgaagttgc ctgttaggct gttggtgctg atgttctgga ttcctgcttc cagcagtgat       60

gttgtgatga cccaaactcc actctccctg cctgtcagtc ttggagatca agcctccatc      120

tcttgcagat ctagtcagag cattgtacac agtaatggaa acacctattt atattggtac      180

ctgcagaaac caggccagtc tccaaagctc ctgatctaca gggtttccaa ccgattttct      240

ggggtcccag acaggttcag tggcagtgga tcagggacag atttcacact caagatcagc      300

agagtggaga ctgaggatat gggaatttat tactgctttc aaggtacaca tgttccgctc      360

acgttcggtg ctgggaccaa gctggagctg aaa                                   393


<210>  41
<211>  1317
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized nucleotide sequence encoding VH domain of 3A5c 
       antibody without leader peptide

<400>  41
caggtccaac tgcagcagcc tggggctgag cttgtgaagc ctggggcttc agtgaagctg       60

tcctgcaagg cttctggcta caccttcacc agctactgga tgcagtgggt aaaacagagg      120

cctggacagg gccttgagtg gatcggagaa attgatcctt ctgatagcta tactaactac      180

aatcaaaagt tcaagggcaa ggccacattg actgtagaca catcctccag cacagcctac      240

atgcagctca gcagcctgac atctgaggac tctgcggtct attactgtgc aagaggggga      300

cttgactact ggggccaagg caccactctc acagtctcct cagccaagac cacacctcct      360

agcgtgtacc ctctggctcc tggatctgcc gctcagacca atagcatggt caccctgggc      420

tgtctggtca agggctactt tcctgagcct gtgaccgtga cctggaacag cggatctctg      480

tctagcggag tgcacacatt ccctgccgtg ctgcagagcg atctgtacac actgagcagc      540

agcgtgacag tgccaagcag ccctagacct agcgagacag tgacctgcaa cgtggcccat      600

cctgccagct ctaccaaggt ggacaagaaa atcgtgccca gagactgcgg ctgcaagccc      660

tgcatctgta cagtgcctga ggtgtccagc gtgttcattt tcccacctaa gcctaaggac      720

gtgctgacca tcacactgac ccctaaagtg acctgtgtgg tggtggacat cagcaaggac      780

gaccccgagg tgcagttcag ttggttcgtg gacgacgtgg aagtgcacac tgcccagaca      840

cagcccagag aggaacagtt caacagcacc ttcagaagcg tgtccgagct gcccatcatg      900

caccaggatt ggctgaacgg caaagagttc aagtgcagag tgaacagcgc cgcctttcct      960

gctcctatcg agaaaacaat cagcaagacc aagggcagac ccaaggctcc ccaggtgtac     1020

acaatccctc cacctaaaga acagatggcc aaggataagg tgtccctgac ctgcatgatc     1080

accgatttct tcccagagga catcaccgtg gaatggcagt ggaatggaca gcccgccgag     1140

aactacaaga acacccagcc tatcatgaac accaacggca gctacttcgt gtacagcaag     1200

ctgaacgtgc agaagtccaa ctgggaggcc ggcaacacct ttacctgtag cgtgctgcac     1260

gagggcctgc acaatcacca caccgagaag tccctgagcc actctcctgg aaagtga        1317


<210>  42
<211>  660
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized nucleotide sequence encoding light chain of 3A5c 
       antibody without leader peptide

<400>  42
gatgttgtga tgacccaaac tccactctcc ctgcctgtca gtcttggaga tcaagcctcc       60

atctcttgca gatctagtca gagcattgta cacagtaatg gaaacaccta tttatattgg      120

tacctgcaga aaccaggcca gtctccaaag ctcctgatct acagggtttc caaccgattt      180

tctggggtcc cagacaggtt cagtggcagt ggatcaggga cagatttcac actcaagatc      240

agcagagtgg agactgagga tatgggaatt tattactgct ttcaaggtac acatgttccg      300

ctcacgttcg gtgctgggac caagctggag ctgaaaagag ctgacgccgc tcctaccgtg      360

tccatctttc cacctagcag cgagcagctg acaagcggcg gagctagcgt cgtgtgcttc      420

ctgaacaact tctaccccaa ggacatcaac gtgaagtgga agatcgacgg cagcgagaga      480

cagaacggcg tgctgaatag ctggaccgac caggacagca aggactccac ctacagcatg      540

tccagcacac tgaccctgac caaggacgag tacgagcggc acaacagcta cacatgcgag      600

gccacacaca agaccagcac aagccccatc gtgaagtcct tcaaccggaa cgagtgctga      660


<210>  43
<211>  1374
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized nucleotide sequence encoding heavy chain of 3A5c 
       antibody with leader peptide

<400>  43
atgggctggt cctgcatcat tctgtttctg gtggccacag ccaccggcgt gcactctcag       60

gtccaactgc agcagcctgg ggctgagctt gtgaagcctg gggcttcagt gaagctgtcc      120

tgcaaggctt ctggctacac cttcaccagc tactggatgc agtgggtaaa acagaggcct      180

ggacagggcc ttgagtggat cggagaaatt gatccttctg atagctatac taactacaat      240

caaaagttca agggcaaggc cacattgact gtagacacat cctccagcac agcctacatg      300

cagctcagca gcctgacatc tgaggactct gcggtctatt actgtgcaag agggggactt      360

gactactggg gccaaggcac cactctcaca gtctcctcag ccaagaccac acctcctagc      420

gtgtaccctc tggctcctgg atctgccgct cagaccaata gcatggtcac cctgggctgt      480

ctggtcaagg gctactttcc tgagcctgtg accgtgacct ggaacagcgg atctctgtct      540

agcggagtgc acacattccc tgccgtgctg cagagcgatc tgtacacact gagcagcagc      600

gtgacagtgc caagcagccc tagacctagc gagacagtga cctgcaacgt ggcccatcct      660

gccagctcta ccaaggtgga caagaaaatc gtgcccagag actgcggctg caagccctgc      720

atctgtacag tgcctgaggt gtccagcgtg ttcattttcc cacctaagcc taaggacgtg      780

ctgaccatca cactgacccc taaagtgacc tgtgtggtgg tggacatcag caaggacgac      840

cccgaggtgc agttcagttg gttcgtggac gacgtggaag tgcacactgc ccagacacag      900

cccagagagg aacagttcaa cagcaccttc agaagcgtgt ccgagctgcc catcatgcac      960

caggattggc tgaacggcaa agagttcaag tgcagagtga acagcgccgc ctttcctgct     1020

cctatcgaga aaacaatcag caagaccaag ggcagaccca aggctcccca ggtgtacaca     1080

atccctccac ctaaagaaca gatggccaag gataaggtgt ccctgacctg catgatcacc     1140

gatttcttcc cagaggacat caccgtggaa tggcagtgga atggacagcc cgccgagaac     1200

tacaagaaca cccagcctat catgaacacc aacggcagct acttcgtgta cagcaagctg     1260

aacgtgcaga agtccaactg ggaggccggc aacaccttta cctgtagcgt gctgcacgag     1320

ggcctgcaca atcaccacac cgagaagtcc ctgagccact ctcctggaaa gtga           1374


<210>  44
<211>  717
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized nucleotide sequence encoding light chain of 3A5c 
       antibody with leader peptide

<400>  44
atgggctggt cctgcatcat tctgtttctg gtggccacag ccaccggcgt gcacagcgat       60

gttgtgatga cccaaactcc actctccctg cctgtcagtc ttggagatca agcctccatc      120

tcttgcagat ctagtcagag cattgtacac agtaatggaa acacctattt atattggtac      180

ctgcagaaac caggccagtc tccaaagctc ctgatctaca gggtttccaa ccgattttct      240

ggggtcccag acaggttcag tggcagtgga tcagggacag atttcacact caagatcagc      300

agagtggaga ctgaggatat gggaatttat tactgctttc aaggtacaca tgttccgctc      360

acgttcggtg ctgggaccaa gctggagctg aaaagagctg acgccgctcc taccgtgtcc      420

atctttccac ctagcagcga gcagctgaca agcggcggag ctagcgtcgt gtgcttcctg      480

aacaacttct accccaagga catcaacgtg aagtggaaga tcgacggcag cgagagacag      540

aacggcgtgc tgaatagctg gaccgaccag gacagcaagg actccaccta cagcatgtcc      600

agcacactga ccctgaccaa ggacgagtac gagcggcaca acagctacac atgcgaggcc      660

acacacaaga ccagcacaag ccccatcgtg aagtccttca accggaacga gtgctga         717


<210>  45
<211>  784
<212>  PRT
<213>  Homo sapiens

<400>  45

Met Gln Arg Leu Met Met Leu Leu Ala Thr Ser Gly Ala Cys Leu Gly 
1               5                   10                  15      


Leu Leu Ala Val Ala Ala Val Ala Ala Ala Gly Ala Asn Pro Ala Gln 
            20                  25                  30          


Arg Asp Thr His Ser Leu Leu Pro Thr His Arg Arg Gln Lys Arg Asp 
        35                  40                  45              


Trp Ile Trp Asn Gln Met His Ile Asp Glu Glu Lys Asn Thr Ser Leu 
    50                  55                  60                  


Pro His His Val Gly Lys Ile Lys Ser Ser Val Ser Arg Lys Asn Ala 
65                  70                  75                  80  


Lys Tyr Leu Leu Lys Gly Glu Tyr Val Gly Lys Val Phe Arg Val Asp 
                85                  90                  95      


Ala Glu Thr Gly Asp Val Phe Ala Ile Glu Arg Leu Asp Arg Glu Asn 
            100                 105                 110         


Ile Ser Glu Tyr His Leu Thr Ala Val Ile Val Asp Lys Asp Thr Gly 
        115                 120                 125             


Glu Asn Leu Glu Thr Pro Ser Ser Phe Thr Ile Lys Val His Asp Val 
    130                 135                 140                 


Asn Asp Asn Trp Pro Val Phe Thr His Arg Leu Phe Asn Ala Ser Val 
145                 150                 155                 160 


Pro Glu Ser Ser Ala Val Gly Thr Ser Val Ile Ser Val Thr Ala Val 
                165                 170                 175     


Asp Ala Asp Asp Pro Thr Val Gly Asp His Ala Ser Val Met Tyr Gln 
            180                 185                 190         


Ile Leu Lys Gly Lys Glu Tyr Phe Ala Ile Asp Asn Ser Gly Arg Ile 
        195                 200                 205             


Ile Thr Ile Thr Lys Ser Leu Asp Arg Glu Lys Gln Ala Arg Tyr Glu 
    210                 215                 220                 


Ile Val Val Glu Ala Arg Asp Ala Gln Gly Leu Arg Gly Asp Ser Gly 
225                 230                 235                 240 


Thr Ala Thr Val Leu Val Thr Leu Gln Asp Ile Asn Asp Asn Phe Pro 
                245                 250                 255     


Phe Phe Thr Gln Thr Lys Tyr Thr Phe Val Val Pro Glu Asp Thr Arg 
            260                 265                 270         


Val Gly Thr Ser Val Gly Ser Leu Phe Val Glu Asp Pro Asp Glu Pro 
        275                 280                 285             


Gln Asn Arg Met Thr Lys Tyr Ser Ile Leu Arg Gly Asp Tyr Gln Asp 
    290                 295                 300                 


Ala Phe Thr Ile Glu Thr Asn Pro Ala His Asn Glu Gly Ile Ile Lys 
305                 310                 315                 320 


Pro Met Lys Pro Leu Asp Tyr Glu Tyr Ile Gln Gln Tyr Ser Phe Ile 
                325                 330                 335     


Val Glu Ala Thr Asp Pro Thr Ile Asp Leu Arg Tyr Met Ser Pro Pro 
            340                 345                 350         


Ala Gly Asn Arg Ala Gln Val Ile Ile Asn Ile Thr Asp Val Asp Glu 
        355                 360                 365             


Pro Pro Ile Phe Gln Gln Pro Phe Tyr His Phe Gln Leu Lys Glu Asn 
    370                 375                 380                 


Gln Lys Lys Pro Leu Ile Gly Thr Val Leu Ala Met Asp Pro Asp Ala 
385                 390                 395                 400 


Ala Arg His Ser Ile Gly Tyr Ser Ile Arg Arg Thr Ser Asp Lys Gly 
                405                 410                 415     


Gln Phe Phe Arg Val Thr Lys Lys Gly Asp Ile Tyr Asn Glu Lys Glu 
            420                 425                 430         


Leu Asp Arg Glu Val Tyr Pro Trp Tyr Asn Leu Thr Val Glu Ala Lys 
        435                 440                 445             


Glu Leu Asp Ser Thr Gly Thr Pro Thr Gly Lys Glu Ser Ile Val Gln 
    450                 455                 460                 


Val His Ile Glu Val Leu Asp Glu Asn Asp Asn Ala Pro Glu Phe Ala 
465                 470                 475                 480 


Lys Pro Tyr Gln Pro Lys Val Cys Glu Asn Ala Val His Gly Gln Leu 
                485                 490                 495     


Val Leu Gln Ile Ser Ala Ile Asp Lys Asp Ile Thr Pro Arg Asn Val 
            500                 505                 510         


Lys Phe Lys Phe Ile Leu Asn Thr Glu Asn Asn Phe Thr Leu Thr Asp 
        515                 520                 525             


Asn His Asp Asn Thr Ala Asn Ile Thr Val Lys Tyr Gly Gln Phe Asp 
    530                 535                 540                 


Arg Glu His Thr Lys Val His Phe Leu Pro Val Val Ile Ser Asp Asn 
545                 550                 555                 560 


Gly Met Pro Ser Arg Thr Gly Thr Ser Thr Leu Thr Val Ala Val Cys 
                565                 570                 575     


Lys Cys Asn Glu Gln Gly Glu Phe Thr Phe Cys Glu Asp Met Ala Ala 
            580                 585                 590         


Gln Val Gly Val Ser Ile Gln Ala Val Val Ala Ile Leu Leu Cys Ile 
        595                 600                 605             


Leu Thr Ile Thr Val Ile Thr Leu Leu Ile Phe Leu Arg Arg Arg Leu 
    610                 615                 620                 


Arg Lys Gln Ala Arg Ala His Gly Lys Ser Val Pro Glu Ile His Glu 
625                 630                 635                 640 


Gln Leu Val Thr Tyr Asp Glu Glu Gly Gly Gly Glu Met Asp Thr Thr 
                645                 650                 655     


Ser Tyr Asp Val Ser Val Leu Asn Ser Val Arg Arg Gly Gly Ala Lys 
            660                 665                 670         


Pro Pro Arg Pro Ala Leu Asp Ala Arg Pro Ser Leu Tyr Ala Gln Val 
        675                 680                 685             


Gln Lys Pro Pro Arg His Ala Pro Gly Ala His Gly Gly Pro Gly Glu 
    690                 695                 700                 


Met Ala Ala Met Ile Glu Val Lys Lys Asp Glu Ala Asp His Asp Gly 
705                 710                 715                 720 


Asp Gly Pro Pro Tyr Asp Thr Leu His Ile Tyr Gly Tyr Glu Gly Ser 
                725                 730                 735     


Glu Ser Ile Ala Glu Ser Leu Ser Ser Leu Gly Thr Asp Ser Ser Asp 
            740                 745                 750         


Ser Asp Val Asp Tyr Asp Phe Leu Asn Asp Trp Gly Pro Arg Phe Lys 
        755                 760                 765             


Met Leu Ala Glu Leu Tyr Gly Ser Asp Pro Arg Glu Glu Leu Leu Tyr 
    770                 775                 780                 


<210>  46
<211>  2355
<212>  DNA
<213>  Homo sapiens

<400>  46
atgcagaggc tcatgatgct cctcgccaca tcgggcgcct gcctgggcct gctggcagtg       60

gcagcagtgg cagcagcagg tgctaaccct gcccaacggg acacccacag cctgctgccc      120

acccaccggc gccaaaagag agattggatt tggaaccaga tgcacattga tgaagagaaa      180

aacacctcac ttccccatca tgtaggcaag atcaagtcaa gcgtgagtcg caagaatgcc      240

aagtacctgc tcaaaggaga atatgtgggc aaggtcttcc gggtcgatgc agagacagga      300

gacgtgttcg ccattgagag gctggaccgg gagaatatct cagagtacca cctcactgct      360

gtcattgtgg acaaggacac tggcgaaaac ctggagactc cttccagctt caccatcaaa      420

gttcatgacg tgaacgacaa ctggcctgtg ttcacgcatc ggttgttcaa tgcgtccgtg      480

cctgagtcgt cggctgtggg gacctcagtc atctctgtga cagcagtgga tgcagacgac      540

cccactgtgg gagaccacgc ctctgtcatg taccaaatcc tgaaggggaa agagtatttt      600

gccatcgata attctggacg tattatcaca ataacgaaaa gcttggaccg agagaagcag      660

gccaggtatg agatcgtggt ggaagcgcga gatgcccagg gcctccgggg ggactcgggc      720

acggccaccg tgctggtcac tctgcaagac atcaatgaca acttcccctt cttcacccag      780

accaagtaca catttgtcgt gcctgaagac acccgtgtgg gcacctctgt gggctctctg      840

tttgttgagg acccagatga gccccagaac cggatgacca agtacagcat cttgcggggc      900

gactaccagg acgctttcac cattgagaca aaccccgccc acaacgaggg catcatcaag      960

cccatgaagc ctctggatta tgaatacatc cagcaataca gcttcatcgt cgaggccaca     1020

gaccccacca tcgacctccg atacatgagc cctcccgcgg gaaacagagc ccaggtcatt     1080

atcaacatca cagatgtgga cgagcccccc attttccagc agcctttcta ccacttccag     1140

ctgaaggaaa accagaagaa gcctctgatt ggcacagtgc tggccatgga ccctgatgcg     1200

gctaggcata gcattggata ctccatccgc aggaccagtg acaagggcca gttcttccga     1260

gtcacaaaaa agggggacat ttacaatgag aaagaactgg acagagaagt ctacccctgg     1320

tataacctga ctgtggaggc caaagaactg gattccactg gaacccccac aggaaaagaa     1380

tccattgtgc aagtccacat tgaagttttg gatgagaatg acaatgcccc ggagtttgcc     1440

aagccctacc agcccaaagt gtgtgagaac gctgtccatg gccagctggt cctgcagatc     1500

tccgcaatag acaaggacat aacaccacga aacgtgaagt tcaaattcat cttgaatact     1560

gagaacaact ttaccctcac ggataatcac gataacacgg ccaacatcac agtcaagtat     1620

gggcagtttg accgggagca taccaaggtc cacttcctac ccgtggtcat ctcagacaat     1680

gggatgccaa gtcgcacggg caccagcacg ctgaccgtgg ccgtgtgcaa gtgcaacgag     1740

cagggcgagt tcaccttctg cgaggatatg gccgcccagg tgggcgtgag catccaggca     1800

gtggtagcca tcttactctg catcctcacc atcacagtga tcaccctgct catcttcctg     1860

cggcggcggc tccggaagca ggcccgcgcg cacggcaaga gcgtgccgga gatccacgag     1920

cagctggtca cctacgacga ggagggcggc ggcgagatgg acaccaccag ctacgatgtg     1980

tcggtgctca actcggtgcg ccgcggcggg gccaagcccc cgcggcccgc gctggacgcc     2040

cggccttccc tctatgcgca ggtgcagaag ccaccgaggc acgcgcctgg ggcacacgga     2100

gggcccgggg agatggcagc catgatcgag gtgaagaagg acgaggcgga ccacgacggc     2160

gacggccccc cctacgacac gctgcacatc tacggctacg agggctccga gtccatagcc     2220

gagtccctca gctccctggg caccgactca tccgactctg acgtggatta cgacttcctt     2280

aacgactggg gacccaggtt taagatgctg gctgagctgt acggctcgga cccccgggag     2340

gagctgctgt attag                                                      2355


<210>  47
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  exemplary synthesized Gly-Ser linker

<400>  47

Gly Gly Ser Gly Gly Gly Ser Gly Gly Ser Gly 
1               5                   10      


<210>  48
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  exemplary synthesized Gly-Ser linker

<400>  48

Gly Gly Ser Gly Gly Gly Ser Gly Ser Gly 
1               5                   10  


<210>  49
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  exemplary synthesized Gly-Ser linker

<400>  49

Gly Gly Ser Gly Gly Gly Ser Gly 
1               5               


<210>  50
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  exemplary synthesized Gly-Ser linker

<400>  50

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


<210>  51
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  exemplary synthesized Gly-Ser linker

<400>  51

Gly Gly Gly Ser Gly Gly Gly Ser 
1               5               


<210>  52
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  exemplary synthesized Gly-Ser linker

<400>  52

Gly Gly Ser Gly Gly Ser 
1               5       


<210>  53
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized CRISPR crRNA sequence used to knock out E-cadherin in
       colo205 cell line

<400>  53
gggatgcata tgggaccacc aag                                               23


<210>  54
<211>  324
<212>  PRT
<213>  Mus musculus

<400>  54

Ala Lys Thr Thr Pro Pro Ser Val Tyr Pro Leu Ala Pro Gly Ser Ala 
1               5                   10                  15      


Ala Gln Thr Asn Ser Met Val Thr Leu Gly Cys Leu Val Lys Gly Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ser Leu Ser Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Asp Leu Tyr Thr Leu 
    50                  55                  60                  


Ser Ser Ser Val Thr Val Pro Ser Ser Pro Arg Pro Ser Glu Thr Val 
65                  70                  75                  80  


Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys Lys 
                85                  90                  95      


Ile Val Pro Arg Asp Cys Gly Cys Lys Pro Cys Ile Cys Thr Val Pro 
            100                 105                 110         


Glu Val Ser Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Val Leu 
        115                 120                 125             


Thr Ile Thr Leu Thr Pro Lys Val Thr Cys Val Val Val Asp Ile Ser 
    130                 135                 140                 


Lys Asp Asp Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp Val Glu 
145                 150                 155                 160 


Val His Thr Ala Gln Thr Gln Pro Arg Glu Glu Gln Phe Asn Ser Thr 
                165                 170                 175     


Phe Arg Ser Val Ser Glu Leu Pro Ile Met His Gln Asp Trp Leu Asn 
            180                 185                 190         


Gly Lys Glu Phe Lys Cys Arg Val Asn Ser Ala Ala Phe Pro Ala Pro 
        195                 200                 205             


Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Arg Pro Lys Ala Pro Gln 
    210                 215                 220                 


Val Tyr Thr Ile Pro Pro Pro Lys Glu Gln Met Ala Lys Asp Lys Val 
225                 230                 235                 240 


Ser Leu Thr Cys Met Ile Thr Asp Phe Phe Pro Glu Asp Ile Thr Val 
                245                 250                 255     


Glu Trp Gln Trp Asn Gly Gln Pro Ala Glu Asn Tyr Lys Asn Thr Gln 
            260                 265                 270         


Pro Ile Met Asn Thr Asn Gly Ser Tyr Phe Val Tyr Ser Lys Leu Asn 
        275                 280                 285             


Val Gln Lys Ser Asn Trp Glu Ala Gly Asn Thr Phe Thr Cys Ser Val 
    290                 295                 300                 


Leu His Glu Gly Leu His Asn His His Thr Glu Lys Ser Leu Ser His 
305                 310                 315                 320 


Ser Pro Gly Lys 
                


<210>  55
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of a twin Strep tag

<400>  55

Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Gly Gly Gly Ser 
1               5                   10                  15      


Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
            20                  25              


<210>  56
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding a twin Strep tag

<400>  56
tggtcccacc cccagtttga gaagggagga ggaagcggag gaggatctgg aggcagcgcc       60

tggtctcacc ctcagttcga gaag                                              84


<210>  57
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain of 8A12c antibody without leader peptide and with 
       twin Strep tag at C-terminus

<400>  57

Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Asn Met His Trp Val Lys Gln Thr Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Glu Tyr Tyr Tyr Gly Ser Ser Phe Asp Phe Trp Gly Gln Gly 
            100                 105                 110         


Thr Thr Leu Thr Val Ser Ser Ala Lys Thr Thr Pro Pro Ser Val Tyr 
        115                 120                 125             


Pro Leu Ala Pro Gly Ser Ala Ala Gln Thr Asn Ser Met Val Thr Leu 
    130                 135                 140                 


Gly Cys Leu Val Lys Gly Tyr Phe Pro Glu Pro Val Thr Val Thr Trp 
145                 150                 155                 160 


Asn Ser Gly Ser Leu Ser Ser Gly Val His Thr Phe Pro Ala Val Leu 
                165                 170                 175     


Gln Ser Asp Leu Tyr Thr Leu Ser Ser Ser Val Thr Val Pro Ser Ser 
            180                 185                 190         


Pro Arg Pro Ser Glu Thr Val Thr Cys Asn Val Ala His Pro Ala Ser 
        195                 200                 205             


Ser Thr Lys Val Asp Lys Lys Ile Val Pro Arg Asp Cys Gly Cys Lys 
    210                 215                 220                 


Pro Cys Ile Cys Thr Val Pro Glu Val Ser Ser Val Phe Ile Phe Pro 
225                 230                 235                 240 


Pro Lys Pro Lys Asp Val Leu Thr Ile Thr Leu Thr Pro Lys Val Thr 
                245                 250                 255     


Cys Val Val Val Asp Ile Ser Lys Asp Asp Pro Glu Val Gln Phe Ser 
            260                 265                 270         


Trp Phe Val Asp Asp Val Glu Val His Thr Ala Gln Thr Gln Pro Arg 
        275                 280                 285             


Glu Glu Gln Phe Asn Ser Thr Phe Arg Ser Val Ser Glu Leu Pro Ile 
    290                 295                 300                 


Met His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys Cys Arg Val Asn 
305                 310                 315                 320 


Ser Ala Ala Phe Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys 
                325                 330                 335     


Gly Arg Pro Lys Ala Pro Gln Val Tyr Thr Ile Pro Pro Pro Lys Glu 
            340                 345                 350         


Gln Met Ala Lys Asp Lys Val Ser Leu Thr Cys Met Ile Thr Asp Phe 
        355                 360                 365             


Phe Pro Glu Asp Ile Thr Val Glu Trp Gln Trp Asn Gly Gln Pro Ala 
    370                 375                 380                 


Glu Asn Tyr Lys Asn Thr Gln Pro Ile Met Asn Thr Asn Gly Ser Tyr 
385                 390                 395                 400 


Phe Val Tyr Ser Lys Leu Asn Val Gln Lys Ser Asn Trp Glu Ala Gly 
                405                 410                 415     


Asn Thr Phe Thr Cys Ser Val Leu His Glu Gly Leu His Asn His His 
            420                 425                 430         


Thr Glu Lys Ser Leu Ser His Ser Pro Gly Lys Gly Ser Glu Asn Leu 
        435                 440                 445             


Tyr Phe Gln Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys Gly Gly 
    450                 455                 460                 


Gly Ser Gly Gly Gly Ser Gly Gly Ser Ala Trp Ser His Pro Gln Phe 
465                 470                 475                 480 


Glu Lys 
        


<210>  58
<211>  1449
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding heavy chain of 8A12c antibody 
       without leader peptide and with twin Strep tag at C-terminus

<400>  58
caggtgcaac tgcagcagcc tggggctgag ctggtgaagc ctggggcctc agtgaagatg       60

tcctgcaagg cttctggcta cacatttacc agttacaata tgcactgggt aaagcagaca      120

cctggacagg gcctggaatg gattggagct atttatccag gaaatggtga tacttcctac      180

aatcagaagt tcaagggcaa ggccacattg actgcagaca aatcctccag cacagcctac      240

atgcagctca gcagcctgac atctgaggac tctgcggtct attactgtgc aagagagtat      300

tactacggta gttcctttga cttctggggc caaggcacca ctctcacagt ctcctcagcc      360

aagaccacac ctcctagcgt gtaccctctg gctcctggat ctgccgctca gaccaatagc      420

atggtcaccc tgggctgtct ggtcaagggc tactttcctg agcctgtgac cgtgacctgg      480

aacagcggat ctctgtctag cggagtgcac acattccctg ccgtgctgca gagcgatctg      540

tacacactga gcagcagcgt gacagtgcca agcagcccta gacctagcga gacagtgacc      600

tgcaacgtgg cccatcctgc cagctctacc aaggtggaca agaaaatcgt gcccagagac      660

tgcggctgca agccctgcat ctgtacagtg cctgaggtgt ccagcgtgtt cattttccca      720

cctaagccta aggacgtgct gaccatcaca ctgaccccta aagtgacctg tgtggtggtg      780

gacatcagca aggacgaccc cgaggtgcag ttcagttggt tcgtggacga cgtggaagtg      840

cacactgccc agacacagcc cagagaggaa cagttcaaca gcaccttcag aagcgtgtcc      900

gagctgccca tcatgcacca ggattggctg aacggcaaag agttcaagtg cagagtgaac      960

agcgccgcct ttcctgctcc tatcgagaaa acaatcagca agaccaaggg cagacccaag     1020

gctccccagg tgtacacaat ccctccacct aaagaacaga tggccaagga taaggtgtcc     1080

ctgacctgca tgatcaccga tttcttccca gaggacatca ccgtggaatg gcagtggaat     1140

ggacagcccg ccgagaacta caagaacacc cagcctatca tgaacaccaa cggcagctac     1200

ttcgtgtaca gcaagctgaa cgtgcagaag tccaactggg aggccggcaa cacctttacc     1260

tgtagcgtgc tgcacgaggg cctgcacaat caccacaccg agaagtccct gagccactct     1320

cctggaaagg gctctgagaa cctgtacttc cagggaagcg cctggtccca cccccagttt     1380

gagaagggag gaggaagcgg aggaggatct ggaggcagcg cctggtctca ccctcagttc     1440

gagaagtga                                                             1449


<210>  59
<211>  501
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Heavy chain of 8A12c antibody with leader peptide and with twin 
       Strep tag at C-terminus

<400>  59

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Ser Tyr Asn Met His Trp Val Lys Gln Thr Pro Gly Gln Gly Leu 
    50                  55                  60                  


Glu Trp Ile Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Glu Tyr Tyr Tyr Gly Ser Ser Phe Asp Phe Trp 
        115                 120                 125             


Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Ala Lys Thr Thr Pro Pro 
    130                 135                 140                 


Ser Val Tyr Pro Leu Ala Pro Gly Ser Ala Ala Gln Thr Asn Ser Met 
145                 150                 155                 160 


Val Thr Leu Gly Cys Leu Val Lys Gly Tyr Phe Pro Glu Pro Val Thr 
                165                 170                 175     


Val Thr Trp Asn Ser Gly Ser Leu Ser Ser Gly Val His Thr Phe Pro 
            180                 185                 190         


Ala Val Leu Gln Ser Asp Leu Tyr Thr Leu Ser Ser Ser Val Thr Val 
        195                 200                 205             


Pro Ser Ser Pro Arg Pro Ser Glu Thr Val Thr Cys Asn Val Ala His 
    210                 215                 220                 


Pro Ala Ser Ser Thr Lys Val Asp Lys Lys Ile Val Pro Arg Asp Cys 
225                 230                 235                 240 


Gly Cys Lys Pro Cys Ile Cys Thr Val Pro Glu Val Ser Ser Val Phe 
                245                 250                 255     


Ile Phe Pro Pro Lys Pro Lys Asp Val Leu Thr Ile Thr Leu Thr Pro 
            260                 265                 270         


Lys Val Thr Cys Val Val Val Asp Ile Ser Lys Asp Asp Pro Glu Val 
        275                 280                 285             


Gln Phe Ser Trp Phe Val Asp Asp Val Glu Val His Thr Ala Gln Thr 
    290                 295                 300                 


Gln Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Ser Val Ser Glu 
305                 310                 315                 320 


Leu Pro Ile Met His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys Cys 
                325                 330                 335     


Arg Val Asn Ser Ala Ala Phe Pro Ala Pro Ile Glu Lys Thr Ile Ser 
            340                 345                 350         


Lys Thr Lys Gly Arg Pro Lys Ala Pro Gln Val Tyr Thr Ile Pro Pro 
        355                 360                 365             


Pro Lys Glu Gln Met Ala Lys Asp Lys Val Ser Leu Thr Cys Met Ile 
    370                 375                 380                 


Thr Asp Phe Phe Pro Glu Asp Ile Thr Val Glu Trp Gln Trp Asn Gly 
385                 390                 395                 400 


Gln Pro Ala Glu Asn Tyr Lys Asn Thr Gln Pro Ile Met Asn Thr Asn 
                405                 410                 415     


Gly Ser Tyr Phe Val Tyr Ser Lys Leu Asn Val Gln Lys Ser Asn Trp 
            420                 425                 430         


Glu Ala Gly Asn Thr Phe Thr Cys Ser Val Leu His Glu Gly Leu His 
        435                 440                 445             


Asn His His Thr Glu Lys Ser Leu Ser His Ser Pro Gly Lys Gly Ser 
    450                 455                 460                 


Glu Asn Leu Tyr Phe Gln Gly Ser Ala Trp Ser His Pro Gln Phe Glu 
465                 470                 475                 480 


Lys Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Ser Ala Trp Ser His 
                485                 490                 495     


Pro Gln Phe Glu Lys 
            500     


<210>  60
<211>  1506
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding heavy chain of 8A12c antibody with 
       leader peptide and with twin Strep tag at C-terminus

<400>  60
atgggctggt cctgcatcat tctgtttctg gtggccacag ccaccggcgt gcactctcag       60

gtgcaactgc agcagcctgg ggctgagctg gtgaagcctg gggcctcagt gaagatgtcc      120

tgcaaggctt ctggctacac atttaccagt tacaatatgc actgggtaaa gcagacacct      180

ggacagggcc tggaatggat tggagctatt tatccaggaa atggtgatac ttcctacaat      240

cagaagttca agggcaaggc cacattgact gcagacaaat cctccagcac agcctacatg      300

cagctcagca gcctgacatc tgaggactct gcggtctatt actgtgcaag agagtattac      360

tacggtagtt cctttgactt ctggggccaa ggcaccactc tcacagtctc ctcagccaag      420

accacacctc ctagcgtgta ccctctggct cctggatctg ccgctcagac caatagcatg      480

gtcaccctgg gctgtctggt caagggctac tttcctgagc ctgtgaccgt gacctggaac      540

agcggatctc tgtctagcgg agtgcacaca ttccctgccg tgctgcagag cgatctgtac      600

acactgagca gcagcgtgac agtgccaagc agccctagac ctagcgagac agtgacctgc      660

aacgtggccc atcctgccag ctctaccaag gtggacaaga aaatcgtgcc cagagactgc      720

ggctgcaagc cctgcatctg tacagtgcct gaggtgtcca gcgtgttcat tttcccacct      780

aagcctaagg acgtgctgac catcacactg acccctaaag tgacctgtgt ggtggtggac      840

atcagcaagg acgaccccga ggtgcagttc agttggttcg tggacgacgt ggaagtgcac      900

actgcccaga cacagcccag agaggaacag ttcaacagca ccttcagaag cgtgtccgag      960

ctgcccatca tgcaccagga ttggctgaac ggcaaagagt tcaagtgcag agtgaacagc     1020

gccgcctttc ctgctcctat cgagaaaaca atcagcaaga ccaagggcag acccaaggct     1080

ccccaggtgt acacaatccc tccacctaaa gaacagatgg ccaaggataa ggtgtccctg     1140

acctgcatga tcaccgattt cttcccagag gacatcaccg tggaatggca gtggaatgga     1200

cagcccgccg agaactacaa gaacacccag cctatcatga acaccaacgg cagctacttc     1260

gtgtacagca agctgaacgt gcagaagtcc aactgggagg ccggcaacac ctttacctgt     1320

agcgtgctgc acgagggcct gcacaatcac cacaccgaga agtccctgag ccactctcct     1380

ggaaagggct ctgagaacct gtacttccag ggaagcgcct ggtcccaccc ccagtttgag     1440

aagggaggag gaagcggagg aggatctgga ggcagcgcct ggtctcaccc tcagttcgag     1500

aagtga                                                                1506


<210>  61
<211>  477
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Heavy chain of 3A5a antibody without leader peptide and with twin
       Strep tag at C-terminus

<400>  61

Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Trp Met Gln Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile Asp Pro Ser Asp Ser Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Thr Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly Leu Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val 
            100                 105                 110         


Ser Ser Ala Lys Thr Thr Pro Pro Ser Val Tyr Pro Leu Ala Pro Gly 
        115                 120                 125             


Ser Ala Ala Gln Thr Asn Ser Met Val Thr Leu Gly Cys Leu Val Lys 
    130                 135                 140                 


Gly Tyr Phe Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ser Leu 
145                 150                 155                 160 


Ser Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Asp Leu Tyr 
                165                 170                 175     


Thr Leu Ser Ser Ser Val Thr Val Pro Ser Ser Pro Arg Pro Ser Glu 
            180                 185                 190         


Thr Val Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp 
        195                 200                 205             


Lys Lys Ile Val Pro Arg Asp Cys Gly Cys Lys Pro Cys Ile Cys Thr 
    210                 215                 220                 


Val Pro Glu Val Ser Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp 
225                 230                 235                 240 


Val Leu Thr Ile Thr Leu Thr Pro Lys Val Thr Cys Val Val Val Asp 
                245                 250                 255     


Ile Ser Lys Asp Asp Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp 
            260                 265                 270         


Val Glu Val His Thr Ala Gln Thr Gln Pro Arg Glu Glu Gln Phe Asn 
        275                 280                 285             


Ser Thr Phe Arg Ser Val Ser Glu Leu Pro Ile Met His Gln Asp Trp 
    290                 295                 300                 


Leu Asn Gly Lys Glu Phe Lys Cys Arg Val Asn Ser Ala Ala Phe Pro 
305                 310                 315                 320 


Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Arg Pro Lys Ala 
                325                 330                 335     


Pro Gln Val Tyr Thr Ile Pro Pro Pro Lys Glu Gln Met Ala Lys Asp 
            340                 345                 350         


Lys Val Ser Leu Thr Cys Met Ile Thr Asp Phe Phe Pro Glu Asp Ile 
        355                 360                 365             


Thr Val Glu Trp Gln Trp Asn Gly Gln Pro Ala Glu Asn Tyr Lys Asn 
    370                 375                 380                 


Thr Gln Pro Ile Met Asn Thr Asn Gly Ser Tyr Phe Val Tyr Ser Lys 
385                 390                 395                 400 


Leu Asn Val Gln Lys Ser Asn Trp Glu Ala Gly Asn Thr Phe Thr Cys 
                405                 410                 415     


Ser Val Leu His Glu Gly Leu His Asn His His Thr Glu Lys Ser Leu 
            420                 425                 430         


Ser His Ser Pro Gly Lys Gly Ser Glu Asn Leu Tyr Phe Gln Gly Ser 
        435                 440                 445             


Ala Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Gly Gly Gly 
    450                 455                 460                 


Ser Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
465                 470                 475         


<210>  62
<211>  1434
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding heavy chain of 3A5a antibody without
       leader peptide and with twin Strep tag at C-terminus

<400>  62
caggtccaac tgcagcagcc tggggctgag cttgtgaagc ctggggcttc agtgaagctg       60

tcctgcaagg cttctggcta caccttcacc agctactgga tgcagtgggt aaaacagagg      120

cctggacagg gccttgagtg gatcggagaa attgatcctt ctgatagcta tactaactac      180

aatcaaaagt tcaagggcaa ggccacattg actgtagaca catcctccag cacagcctac      240

atgcagctca gcagcctgac atctgaggac tctgcggtct attactgtgc aagaggggga      300

cttgactact ggggccaagg caccactctc acagtctcct cagccaagac cacacctcct      360

agcgtgtacc ctctggctcc tggatctgcc gctcagacca atagcatggt caccctgggc      420

tgtctggtca agggctactt tcctgagcct gtgaccgtga cctggaacag cggatctctg      480

tctagcggag tgcacacatt ccctgccgtg ctgcagagcg atctgtacac actgagcagc      540

agcgtgacag tgccaagcag ccctagacct agcgagacag tgacctgcaa cgtggcccat      600

cctgccagct ctaccaaggt ggacaagaaa atcgtgccca gagactgcgg ctgcaagccc      660

tgcatctgta cagtgcctga ggtgtccagc gtgttcattt tcccacctaa gcctaaggac      720

gtgctgacca tcacactgac ccctaaagtg acctgtgtgg tggtggacat cagcaaggac      780

gaccccgagg tgcagttcag ttggttcgtg gacgacgtgg aagtgcacac tgcccagaca      840

cagcccagag aggaacagtt caacagcacc ttcagaagcg tgtccgagct gcccatcatg      900

caccaggatt ggctgaacgg caaagagttc aagtgcagag tgaacagcgc cgcctttcct      960

gctcctatcg agaaaacaat cagcaagacc aagggcagac ccaaggctcc ccaggtgtac     1020

acaatccctc cacctaaaga acagatggcc aaggataagg tgtccctgac ctgcatgatc     1080

accgatttct tcccagagga catcaccgtg gaatggcagt ggaatggaca gcccgccgag     1140

aactacaaga acacccagcc tatcatgaac accaacggca gctacttcgt gtacagcaag     1200

ctgaacgtgc agaagtccaa ctgggaggcc ggcaacacct ttacctgtag cgtgctgcac     1260

gagggcctgc acaatcacca caccgagaag tccctgagcc actctcctgg aaagggctct     1320

gagaacctgt acttccaggg aagcgcctgg tcccaccccc agtttgagaa gggaggagga     1380

agcggaggag gatctggagg cagcgcctgg tctcaccctc agttcgagaa gtga           1434


<210>  63
<211>  496
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Heavy chain of 3A5a antibody with leader peptide and with twin 
       Strep tag at C-terminus

<400>  63

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Ser Tyr Trp Met Gln Trp Val Lys Gln Arg Pro Gly Gln Gly Leu 
    50                  55                  60                  


Glu Trp Ile Gly Glu Ile Asp Pro Ser Asp Ser Tyr Thr Asn Tyr Asn 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Val Asp Thr Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Gly Gly Leu Asp Tyr Trp Gly Gln Gly Thr Thr 
        115                 120                 125             


Leu Thr Val Ser Ser Ala Lys Thr Thr Pro Pro Ser Val Tyr Pro Leu 
    130                 135                 140                 


Ala Pro Gly Ser Ala Ala Gln Thr Asn Ser Met Val Thr Leu Gly Cys 
145                 150                 155                 160 


Leu Val Lys Gly Tyr Phe Pro Glu Pro Val Thr Val Thr Trp Asn Ser 
                165                 170                 175     


Gly Ser Leu Ser Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser 
            180                 185                 190         


Asp Leu Tyr Thr Leu Ser Ser Ser Val Thr Val Pro Ser Ser Pro Arg 
        195                 200                 205             


Pro Ser Glu Thr Val Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr 
    210                 215                 220                 


Lys Val Asp Lys Lys Ile Val Pro Arg Asp Cys Gly Cys Lys Pro Cys 
225                 230                 235                 240 


Ile Cys Thr Val Pro Glu Val Ser Ser Val Phe Ile Phe Pro Pro Lys 
                245                 250                 255     


Pro Lys Asp Val Leu Thr Ile Thr Leu Thr Pro Lys Val Thr Cys Val 
            260                 265                 270         


Val Val Asp Ile Ser Lys Asp Asp Pro Glu Val Gln Phe Ser Trp Phe 
        275                 280                 285             


Val Asp Asp Val Glu Val His Thr Ala Gln Thr Gln Pro Arg Glu Glu 
    290                 295                 300                 


Gln Phe Asn Ser Thr Phe Arg Ser Val Ser Glu Leu Pro Ile Met His 
305                 310                 315                 320 


Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys Cys Arg Val Asn Ser Ala 
                325                 330                 335     


Ala Phe Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Arg 
            340                 345                 350         


Pro Lys Ala Pro Gln Val Tyr Thr Ile Pro Pro Pro Lys Glu Gln Met 
        355                 360                 365             


Ala Lys Asp Lys Val Ser Leu Thr Cys Met Ile Thr Asp Phe Phe Pro 
    370                 375                 380                 


Glu Asp Ile Thr Val Glu Trp Gln Trp Asn Gly Gln Pro Ala Glu Asn 
385                 390                 395                 400 


Tyr Lys Asn Thr Gln Pro Ile Met Asn Thr Asn Gly Ser Tyr Phe Val 
                405                 410                 415     


Tyr Ser Lys Leu Asn Val Gln Lys Ser Asn Trp Glu Ala Gly Asn Thr 
            420                 425                 430         


Phe Thr Cys Ser Val Leu His Glu Gly Leu His Asn His His Thr Glu 
        435                 440                 445             


Lys Ser Leu Ser His Ser Pro Gly Lys Gly Ser Glu Asn Leu Tyr Phe 
    450                 455                 460                 


Gln Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser 
465                 470                 475                 480 


Gly Gly Gly Ser Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
                485                 490                 495     


<210>  64
<211>  1491
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding heavy chain of 3A5a antibody with 
       leader peptide and with twin Strep tag at C-terminus

<400>  64
atgggctggt cctgcatcat tctgtttctg gtggccacag ccaccggcgt gcactctcag       60

gtccaactgc agcagcctgg ggctgagctt gtgaagcctg gggcttcagt gaagctgtcc      120

tgcaaggctt ctggctacac cttcaccagc tactggatgc agtgggtaaa acagaggcct      180

ggacagggcc ttgagtggat cggagaaatt gatccttctg atagctatac taactacaat      240

caaaagttca agggcaaggc cacattgact gtagacacat cctccagcac agcctacatg      300

cagctcagca gcctgacatc tgaggactct gcggtctatt actgtgcaag agggggactt      360

gactactggg gccaaggcac cactctcaca gtctcctcag ccaagaccac acctcctagc      420

gtgtaccctc tggctcctgg atctgccgct cagaccaata gcatggtcac cctgggctgt      480

ctggtcaagg gctactttcc tgagcctgtg accgtgacct ggaacagcgg atctctgtct      540

agcggagtgc acacattccc tgccgtgctg cagagcgatc tgtacacact gagcagcagc      600

gtgacagtgc caagcagccc tagacctagc gagacagtga cctgcaacgt ggcccatcct      660

gccagctcta ccaaggtgga caagaaaatc gtgcccagag actgcggctg caagccctgc      720

atctgtacag tgcctgaggt gtccagcgtg ttcattttcc cacctaagcc taaggacgtg      780

ctgaccatca cactgacccc taaagtgacc tgtgtggtgg tggacatcag caaggacgac      840

cccgaggtgc agttcagttg gttcgtggac gacgtggaag tgcacactgc ccagacacag      900

cccagagagg aacagttcaa cagcaccttc agaagcgtgt ccgagctgcc catcatgcac      960

caggattggc tgaacggcaa agagttcaag tgcagagtga acagcgccgc ctttcctgct     1020

cctatcgaga aaacaatcag caagaccaag ggcagaccca aggctcccca ggtgtacaca     1080

atccctccac ctaaagaaca gatggccaag gataaggtgt ccctgacctg catgatcacc     1140

gatttcttcc cagaggacat caccgtggaa tggcagtgga atggacagcc cgccgagaac     1200

tacaagaaca cccagcctat catgaacacc aacggcagct acttcgtgta cagcaagctg     1260

aacgtgcaga agtccaactg ggaggccggc aacaccttta cctgtagcgt gctgcacgag     1320

ggcctgcaca atcaccacac cgagaagtcc ctgagccact ctcctggaaa gggctctgag     1380

aacctgtact tccagggaag cgcctggtcc cacccccagt ttgagaaggg aggaggaagc     1440

ggaggaggat ctggaggcag cgcctggtct caccctcagt tcgagaagtg a              1491


<210>  65
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of a signal peptide

<400>  65

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser 
            


<210>  66
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding a signal peptide of SEQ ID NO: 65

<400>  66
atgggctggt cctgcatcat tctgtttctg gtggccacag ccaccggcgt gcactct          57


<210>  67
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of a signal peptide

<400>  67

Met Lys Leu Pro Val Arg Leu Leu Val Leu Met Phe Trp Ile Pro Ala 
1               5                   10                  15      


Ser Ser Ser 
            


<210>  68
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding a signal peptide of SEQ ID NO: 67

<400>  68
atgaagttgc ctgttaggct gttggtgctg atgttctgga ttcctgcttc cagcagt          57


