                         SEQUENCE LISTING

<110>  WuXi Biologics (Shanghai) CO., LTD.
       WuXi Biologics Ireland Limited
 
<120>  Surface display of antibodies in yeast cell

<130>  AJ3297PCT2104

<160>  13    

<170>  PatentIn version 3.3

<210>  1
<211>  725
<212>  PRT
<213>  Artificial

<220>
<223>  ScAgal

<400>  1

Met Thr Leu Ser Phe Ala His Phe Thr Tyr Leu Phe Thr Ile Leu Leu 
1               5                   10                  15      


Gly Leu Thr Asn Ile Ala Leu Ala Ser Asp Pro Glu Thr Ile Leu Val 
            20                  25                  30          


Thr Ile Thr Lys Thr Asn Asp Ala Asn Gly Val Val Thr Thr Thr Val 
        35                  40                  45              


Ser Pro Ala Leu Val Ser Thr Ser Thr Ile Val Gln Ala Gly Thr Thr 
    50                  55                  60                  


Thr Leu Tyr Thr Thr Trp Cys Pro Leu Thr Val Ser Thr Ser Ser Ala 
65                  70                  75                  80  


Ala Glu Ile Ser Pro Ser Ile Ser Tyr Ala Thr Thr Leu Ser Arg Phe 
                85                  90                  95      


Ser Thr Leu Thr Leu Ser Thr Glu Val Cys Ser His Glu Ala Cys Pro 
            100                 105                 110         


Ser Ser Ser Thr Leu Pro Thr Thr Thr Leu Ser Val Thr Ser Lys Phe 
        115                 120                 125             


Thr Ser Tyr Ile Cys Pro Thr Cys His Thr Thr Ala Ile Ser Ser Leu 
    130                 135                 140                 


Ser Glu Val Gly Thr Thr Thr Val Val Ser Ser Ser Ala Ile Glu Pro 
145                 150                 155                 160 


Ser Ser Ala Ser Ile Ile Ser Pro Val Thr Ser Thr Leu Ser Ser Thr 
                165                 170                 175     


Thr Ser Ser Asn Pro Thr Thr Thr Ser Leu Ser Ser Thr Ser Thr Ser 
            180                 185                 190         


Pro Ser Ser Thr Ser Thr Ser Pro Ser Ser Thr Ser Thr Ser Ser Ser 
        195                 200                 205             


Ser Thr Ser Thr Ser Ser Ser Ser Thr Ser Thr Ser Ser Ser Ser Thr 
    210                 215                 220                 


Ser Thr Ser Pro Ser Ser Thr Ser Thr Ser Ser Ser Leu Thr Ser Thr 
225                 230                 235                 240 


Ser Ser Ser Ser Thr Ser Thr Ser Gln Ser Ser Thr Ser Thr Ser Ser 
                245                 250                 255     


Ser Ser Thr Ser Thr Ser Pro Ser Ser Thr Ser Thr Ser Ser Ser Ser 
            260                 265                 270         


Thr Ser Thr Ser Pro Ser Ser Lys Ser Thr Ser Ala Ser Ser Thr Ser 
        275                 280                 285             


Thr Ser Ser Tyr Ser Thr Ser Thr Ser Pro Ser Leu Thr Ser Ser Ser 
    290                 295                 300                 


Pro Thr Leu Ala Ser Thr Ser Pro Ser Ser Thr Ser Ile Ser Ser Thr 
305                 310                 315                 320 


Phe Thr Asp Ser Thr Ser Ser Leu Gly Ser Ser Ile Ala Ser Ser Ser 
                325                 330                 335     


Thr Ser Val Ser Leu Tyr Ser Pro Ser Thr Pro Val Tyr Ser Val Pro 
            340                 345                 350         


Ser Thr Ser Ser Asn Val Ala Thr Pro Ser Met Thr Ser Ser Thr Val 
        355                 360                 365             


Glu Thr Thr Val Ser Ser Gln Ser Ser Ser Glu Tyr Ile Thr Lys Ser 
    370                 375                 380                 


Ser Ile Ser Thr Thr Ile Pro Ser Phe Ser Met Ser Thr Tyr Phe Thr 
385                 390                 395                 400 


Thr Val Ser Gly Val Thr Thr Met Tyr Thr Thr Trp Cys Pro Tyr Ser 
                405                 410                 415     


Ser Glu Ser Glu Thr Ser Thr Leu Thr Ser Met His Glu Thr Val Thr 
            420                 425                 430         


Thr Asp Ala Thr Val Cys Thr His Glu Ser Cys Met Pro Ser Gln Thr 
        435                 440                 445             


Thr Ser Leu Ile Thr Ser Ser Ile Lys Met Ser Thr Lys Asn Val Ala 
    450                 455                 460                 


Thr Ser Val Ser Thr Ser Thr Val Glu Ser Ser Tyr Ala Cys Ser Thr 
465                 470                 475                 480 


Cys Ala Glu Thr Ser His Ser Tyr Ser Ser Val Gln Thr Ala Ser Ser 
                485                 490                 495     


Ser Ser Val Thr Gln Gln Thr Thr Ser Thr Lys Ser Trp Val Ser Ser 
            500                 505                 510         


Met Thr Thr Ser Asp Glu Asp Phe Asn Lys His Ala Thr Gly Lys Tyr 
        515                 520                 525             


His Val Thr Ser Ser Gly Thr Ser Thr Ile Ser Thr Ser Val Ser Glu 
    530                 535                 540                 


Ala Thr Ser Thr Ser Ser Ile Asp Ser Glu Ser Gln Glu Gln Ser Ser 
545                 550                 555                 560 


His Leu Leu Ser Thr Ser Val Leu Ser Ser Ser Ser Leu Ser Ala Thr 
                565                 570                 575     


Leu Ser Ser Asp Ser Thr Ile Leu Leu Phe Ser Ser Val Ser Ser Leu 
            580                 585                 590         


Ser Val Glu Gln Ser Pro Val Thr Thr Leu Gln Ile Ser Ser Thr Ser 
        595                 600                 605             


Glu Ile Leu Gln Pro Thr Ser Ser Thr Ala Ile Ala Thr Ile Ser Ala 
    610                 615                 620                 


Ser Thr Ser Ser Leu Ser Ala Thr Ser Ile Ser Thr Pro Ser Thr Ser 
625                 630                 635                 640 


Val Glu Ser Thr Ile Glu Ser Ser Ser Leu Thr Pro Thr Val Ser Ser 
                645                 650                 655     


Ile Phe Leu Ser Ser Ser Ser Ala Pro Ser Ser Leu Gln Thr Ser Val 
            660                 665                 670         


Thr Thr Thr Glu Val Ser Thr Thr Ser Ile Ser Ile Gln Tyr Gln Thr 
        675                 680                 685             


Ser Ser Met Val Thr Ile Ser Gln Tyr Met Gly Ser Gly Ser Gln Thr 
    690                 695                 700                 


Arg Leu Pro Leu Gly Lys Leu Val Phe Ala Ile Met Ala Val Ala Cys 
705                 710                 715                 720 


Asn Val Ile Phe Ser 
                725 


<210>  2
<211>  69
<212>  PRT
<213>  Artificial

<220>
<223>  ScAga2 subunit

<400>  2

Gln Glu Leu Thr Thr Ile Cys Glu Gln Ile Pro Ser Pro Thr Leu Glu 
1               5                   10                  15      


Ser Thr Pro Tyr Ser Leu Ser Thr Thr Thr Ile Leu Ala Asn Gly Lys 
            20                  25                  30          


Ala Met Gln Gly Val Phe Glu Tyr Tyr Lys Ser Val Thr Phe Val Ser 
        35                  40                  45              


Asn Cys Gly Ser His Pro Ser Thr Thr Ser Lys Gly Ser Pro Ile Asn 
    50                  55                  60                  


Thr Gln Tyr Val Phe 
65                  


<210>  3
<211>  15
<212>  PRT
<213>  Artificial

<220>
<223>  Linker

<400>  3

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  4
<211>  18
<212>  PRT
<213>  Artificial

<220>
<223>  ER targeting peptide

<400>  4

Met Gln Leu Leu Arg Cys Phe Ser Ile Phe Ser Val Ile Ala Ser Val 
1               5                   10                  15      


Leu Ala 
        


<210>  5
<211>  327
<212>  PRT
<213>  Artificial

<220>
<223>  IgG4_S228P constant region

<400>  5

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro 
            100                 105                 110         


Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
        195                 200                 205             


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
225                 230                 235                 240 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
                245                 250                 255     


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
            260                 265                 270         


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
        275                 280                 285             


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
    290                 295                 300                 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
305                 310                 315                 320 


Leu Ser Leu Ser Leu Gly Lys 
                325         


<210>  6
<211>  107
<212>  PRT
<213>  Artificial

<220>
<223>  Light chain kappa constant region

<400>  6

Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         


<210>  7
<211>  106
<212>  PRT
<213>  Artificial

<220>
<223>  Light chain lambda constant region

<400>  7

Gly Gln Pro Lys Ala Ala Pro Ser Val Thr Leu Phe Pro Pro Ser Ser 
1               5                   10                  15      


Glu Glu Leu Gln Ala Asn Lys Ala Thr Leu Val Cys Leu Ile Ser Asp 
            20                  25                  30          


Phe Tyr Pro Gly Ala Val Thr Val Ala Trp Lys Ala Asp Ser Ser Pro 
        35                  40                  45              


Val Lys Ala Gly Val Glu Thr Thr Thr Pro Ser Lys Gln Ser Asn Asn 
    50                  55                  60                  


Lys Tyr Ala Ala Ser Ser Tyr Leu Ser Leu Thr Pro Glu Gln Trp Lys 
65                  70                  75                  80  


Ser His Arg Ser Tyr Ser Cys Gln Val Thr His Glu Gly Ser Thr Val 
                85                  90                  95      


Glu Lys Thr Val Ala Pro Thr Glu Cys Ser 
            100                 105     


<210>  8
<211>  89
<212>  PRT
<213>  Artificial

<220>
<223>  Secretion signal peptide

<400>  8

Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
1               5                   10                  15      


Ala Leu Ala Ala Pro Ala Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln 
            20                  25                  30          


Ile Pro Ala Glu Ala Val Ile Asp Tyr Ser Asp Leu Glu Gly Asp Phe 
        35                  40                  45              


Asp Ala Ala Ala Leu Pro Leu Ser Asn Ser Thr Asn Asn Gly Leu Ser 
    50                  55                  60                  


Ser Thr Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val 
65                  70                  75                  80  


Gln Leu Glu Lys Arg Glu Ala Glu Ala 
                85                  


<210>  9
<211>  330
<212>  PRT
<213>  Artificial

<220>
<223>  IgG1 constant region

<400>  9

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
225                 230                 235                 240 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  10
<211>  522
<212>  PRT
<213>  Artificial

<220>
<223>  Molecular chaperone PDI

<400>  10

Met Lys Phe Ser Ala Gly Ala Val Leu Ser Trp Ser Ser Leu Leu Leu 
1               5                   10                  15      


Ala Ser Ser Val Phe Ala Gln Gln Glu Ala Val Ala Pro Glu Asp Ser 
            20                  25                  30          


Ala Val Val Lys Leu Ala Thr Asp Ser Phe Asn Glu Tyr Ile Gln Ser 
        35                  40                  45              


His Asp Leu Val Leu Ala Glu Phe Phe Ala Pro Trp Cys Gly His Cys 
    50                  55                  60                  


Lys Asn Met Ala Pro Glu Tyr Val Lys Ala Ala Glu Thr Leu Val Glu 
65                  70                  75                  80  


Lys Asn Ile Thr Leu Ala Gln Ile Asp Cys Thr Glu Asn Gln Asp Leu 
                85                  90                  95      


Cys Met Glu His Asn Ile Pro Gly Phe Pro Ser Leu Lys Ile Phe Lys 
            100                 105                 110         


Asn Ser Asp Val Asn Asn Ser Ile Asp Tyr Glu Gly Pro Arg Thr Ala 
        115                 120                 125             


Glu Ala Ile Val Gln Phe Met Ile Lys Gln Ser Gln Pro Ala Val Ala 
    130                 135                 140                 


Val Val Ala Asp Leu Pro Ala Tyr Leu Ala Asn Glu Thr Phe Val Thr 
145                 150                 155                 160 


Pro Val Ile Val Gln Ser Gly Lys Ile Asp Ala Asp Phe Asn Ala Thr 
                165                 170                 175     


Phe Tyr Ser Met Ala Asn Lys His Phe Asn Asp Tyr Asp Phe Val Ser 
            180                 185                 190         


Ala Glu Asn Ala Asp Asp Asp Phe Lys Leu Ser Ile Tyr Leu Pro Ser 
        195                 200                 205             


Ala Met Asp Glu Pro Val Val Tyr Asn Gly Lys Lys Ala Asp Ile Ala 
    210                 215                 220                 


Asp Ala Asp Val Phe Glu Lys Trp Leu Gln Val Glu Ala Leu Pro Tyr 
225                 230                 235                 240 


Phe Gly Glu Ile Asp Gly Ser Val Phe Ala Gln Tyr Val Glu Ser Gly 
                245                 250                 255     


Leu Pro Leu Gly Tyr Leu Phe Tyr Asn Asp Glu Glu Glu Leu Glu Glu 
            260                 265                 270         


Tyr Lys Pro Leu Phe Thr Glu Leu Ala Lys Lys Asn Arg Gly Leu Met 
        275                 280                 285             


Asn Phe Val Ser Ile Asp Ala Arg Lys Phe Gly Arg His Ala Gly Asn 
    290                 295                 300                 


Leu Asn Met Lys Glu Gln Phe Pro Leu Phe Ala Ile His Asp Met Thr 
305                 310                 315                 320 


Glu Asp Leu Lys Tyr Gly Leu Pro Gln Leu Ser Glu Glu Ala Phe Asp 
                325                 330                 335     


Glu Leu Ser Asp Lys Ile Val Leu Glu Ser Lys Ala Ile Glu Ser Leu 
            340                 345                 350         


Val Lys Asp Phe Leu Lys Gly Asp Ala Ser Pro Ile Val Lys Ser Gln 
        355                 360                 365             


Glu Ile Phe Glu Asn Gln Asp Ser Ser Val Phe Gln Leu Val Gly Lys 
    370                 375                 380                 


Asn His Asp Glu Ile Val Asn Asp Pro Lys Lys Asp Val Leu Val Leu 
385                 390                 395                 400 


Tyr Tyr Ala Pro Trp Cys Gly His Cys Lys Arg Leu Ala Pro Thr Tyr 
                405                 410                 415     


Gln Glu Leu Ala Asp Thr Tyr Ala Asn Ala Thr Ser Asp Val Leu Ile 
            420                 425                 430         


Ala Lys Leu Asp His Thr Glu Asn Asp Val Arg Gly Val Val Ile Glu 
        435                 440                 445             


Gly Tyr Pro Thr Ile Val Leu Tyr Pro Gly Gly Lys Lys Ser Glu Ser 
    450                 455                 460                 


Val Val Tyr Gln Gly Ser Arg Ser Leu Asp Ser Leu Phe Asp Phe Ile 
465                 470                 475                 480 


Lys Glu Asn Gly His Phe Asp Val Asp Gly Lys Ala Leu Tyr Glu Glu 
                485                 490                 495     


Ala Gln Glu Lys Ala Ala Glu Glu Ala Asp Ala Asp Ala Glu Leu Ala 
            500                 505                 510         


Asp Glu Glu Asp Ala Ile His Asp Glu Leu 
        515                 520         


<210>  11
<211>  678
<212>  PRT
<213>  Artificial

<220>
<223>  Molecular chaperone BiP

<400>  11

Met Phe Phe Asn Arg Leu Ser Ala Gly Lys Leu Leu Val Pro Leu Ser 
1               5                   10                  15      


Val Val Leu Tyr Ala Leu Phe Val Val Ile Leu Pro Leu Gln Asn Ser 
            20                  25                  30          


Phe His Ser Ser Asn Val Leu Val Arg Gly Glu Glu Glu Asp Lys Lys 
        35                  40                  45              


Glu Asp Val Gly Thr Val Val Gly Ile Asp Leu Gly Thr Thr Tyr Ser 
    50                  55                  60                  


Cys Val Gly Val Phe Lys Asn Gly Arg Val Glu Ile Ile Ala Asn Asp 
65                  70                  75                  80  


Gln Gly Asn Arg Ile Thr Pro Ser Tyr Val Ala Phe Thr Pro Glu Gly 
                85                  90                  95      


Glu Arg Leu Ile Gly Asp Ala Ala Lys Asn Gln Leu Thr Ser Asn Pro 
            100                 105                 110         


Glu Asn Thr Val Phe Asp Ala Lys Arg Leu Ile Gly Arg Thr Trp Asn 
        115                 120                 125             


Asp Pro Ser Val Gln Gln Asp Ile Lys Phe Leu Pro Phe Lys Val Val 
    130                 135                 140                 


Glu Lys Lys Thr Lys Pro Tyr Ile Gln Val Asp Ile Gly Gly Gly Gln 
145                 150                 155                 160 


Thr Lys Thr Phe Ala Pro Glu Glu Ile Ser Ala Met Val Leu Thr Lys 
                165                 170                 175     


Met Lys Glu Thr Ala Glu Ala Tyr Leu Gly Lys Lys Val Thr His Ala 
            180                 185                 190         


Val Val Thr Val Pro Ala Tyr Phe Asn Asp Ala Gln Arg Gln Ala Thr 
        195                 200                 205             


Lys Asp Ala Gly Thr Ile Ala Gly Leu Asn Val Met Arg Ile Ile Asn 
    210                 215                 220                 


Glu Pro Thr Ala Ala Ala Ile Ala Tyr Gly Leu Asp Lys Arg Glu Gly 
225                 230                 235                 240 


Glu Lys Asn Ile Leu Val Phe Asp Leu Gly Gly Gly Thr Phe Asp Val 
                245                 250                 255     


Ser Leu Leu Thr Ile Asp Asn Gly Val Phe Glu Val Val Ala Thr Asn 
            260                 265                 270         


Gly Asp Thr His Leu Gly Gly Glu Asp Phe Asp Gln Arg Val Met Glu 
        275                 280                 285             


His Phe Ile Lys Leu Tyr Lys Lys Lys Thr Gly Lys Asp Val Arg Lys 
    290                 295                 300                 


Asp Asn Arg Ala Val Gln Lys Leu Arg Arg Glu Val Glu Lys Ala Lys 
305                 310                 315                 320 


Arg Ala Leu Ser Ser Gln His Gln Ala Arg Ile Glu Ile Glu Ser Phe 
                325                 330                 335     


Tyr Glu Gly Glu Asp Phe Ser Glu Thr Leu Thr Arg Ala Lys Phe Glu 
            340                 345                 350         


Glu Leu Asn Met Asp Leu Phe Arg Ser Thr Met Lys Pro Val Gln Lys 
        355                 360                 365             


Val Leu Glu Asp Ser Asp Leu Lys Lys Ser Asp Ile Asp Glu Ile Val 
    370                 375                 380                 


Leu Val Gly Gly Ser Thr Arg Ile Pro Lys Ile Gln Gln Leu Val Lys 
385                 390                 395                 400 


Glu Phe Phe Asn Gly Lys Glu Pro Ser Arg Gly Ile Asn Pro Asp Glu 
                405                 410                 415     


Ala Val Ala Tyr Gly Ala Ala Val Gln Ala Gly Val Leu Ser Gly Asp 
            420                 425                 430         


Gln Asp Thr Gly Asp Leu Val Leu Leu Asp Val Cys Pro Leu Thr Leu 
        435                 440                 445             


Gly Ile Glu Thr Val Gly Gly Val Met Thr Lys Leu Ile Pro Arg Asn 
    450                 455                 460                 


Thr Val Val Pro Thr Lys Lys Ser Gln Ile Phe Ser Thr Ala Ser Asp 
465                 470                 475                 480 


Asn Gln Pro Thr Val Thr Ile Lys Val Tyr Glu Gly Glu Arg Pro Leu 
                485                 490                 495     


Thr Lys Asp Asn His Leu Leu Gly Thr Phe Asp Leu Thr Gly Ile Pro 
            500                 505                 510         


Pro Ala Pro Arg Gly Val Pro Gln Ile Glu Val Thr Phe Glu Ile Asp 
        515                 520                 525             


Val Asn Gly Ile Leu Arg Val Thr Ala Glu Asp Lys Gly Thr Gly Asn 
    530                 535                 540                 


Lys Asn Lys Ile Thr Ile Thr Asn Asp Gln Asn Arg Leu Thr Pro Glu 
545                 550                 555                 560 


Glu Ile Glu Arg Met Val Asn Asp Ala Glu Lys Phe Ala Glu Glu Asp 
                565                 570                 575     


Lys Lys Leu Lys Glu Arg Ile Asp Thr Arg Asn Glu Leu Glu Ser Tyr 
            580                 585                 590         


Ala Tyr Ser Leu Lys Asn Gln Ile Gly Asp Lys Glu Lys Leu Gly Gly 
        595                 600                 605             


Lys Leu Ser Ser Glu Asp Lys Glu Thr Met Glu Lys Ala Val Glu Glu 
    610                 615                 620                 


Lys Ile Glu Trp Leu Glu Ser His Gln Asp Ala Asp Ile Glu Asp Phe 
625                 630                 635                 640 


Lys Ala Lys Lys Lys Glu Leu Glu Glu Ile Val Gln Pro Ile Ile Ser 
                645                 650                 655     


Lys Leu Tyr Gly Ser Ala Gly Pro Pro Pro Thr Gly Glu Glu Asp Thr 
            660                 665                 670         


Ala Glu Lys Asp Glu Leu 
        675             


<210>  12
<211>  124
<212>  PRT
<213>  Artificial

<220>
<223>  IgG_VH

<400>  12

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Arg Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Val Thr Phe Asp Asp Tyr 
            20                  25                  30          


Gly Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Gly Ile Asn Trp Asn Gly Gly Asp Thr Asp Tyr Ser Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Leu Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Asp Phe Tyr Gly Ser Gly Ser Tyr Tyr His Val Pro Phe Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Ile Leu Val Thr Val Ser Ser 
        115                 120                 


<210>  13
<211>  108
<212>  PRT
<213>  Artificial

<220>
<223>  IgG_VL

<400>  13

Glu Ile Val Leu Thr Gln Ser Pro Gly Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Ser Arg Ser 
            20                  25                  30          


Tyr Leu Ala Trp Tyr Gln Gln Lys Arg Gly Gln Ala Pro Arg Leu Leu 
        35                  40                  45              


Ile Tyr Gly Ala Ser Ser Arg Ala Thr Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Asp Gly Ser Gly Thr Asp Phe Thr Leu Ser Ile Ser Arg Leu Glu 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Val Tyr Tyr Cys His Gln Tyr Asp Met Ser Pro 
                85                  90                  95      


Phe Thr Phe Gly Pro Gly Thr Lys Val Asp Ile Lys 
            100                 105             


