﻿                         序列表

<110>  杭州观苏生物技术有限公司

<120>  瘤内注射变异胶原酶治疗癌症的方法

<150>  202011596224.5
<151>  2020-12-29

<160>  2

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  2946
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  1
atggttcaga atgagagtaa acgttatacc gttagctacc tgaaaacact gaactattat        60
gatctggtgg atctgctggt gaagacagag attgaaaact tacctgatct gttccagtac       120
agcagcgatg ccaaagagtt ttacggcaac aagacccgta tgagcttcat catggacgag       180
attggccgtc gtgcccctca gtataccgaa atcgaccata agggcatccc gaccctggtt       240
gaggttgtgc gtgccggttt ctacctgggc ttccacaata aagagctgaa cgaaatcaat       300
aaacgtagct ttaaggagcg cgtgattcct agtattctgg ccattcagaa aaatcctaat       360
ttcaagctgg gtactgaagt gcaggacaaa atcgttagcg ccacggggtt actggcaggc       420
aatgaaaccg ccccgccgga ggttgtgaat aacttcaccc cgatcttgca ggactgcatc       480
aaaaacatcg accgctatgc cctggacgac ctgaaaagta aggcactgtt caacgtgctg       540
gccgcaccta catacgatat cacagaatac ttacgcgcca ccaaggagaa accggagaat       600
accccgtggt acggcaagat tgacggcttc attaacgagc tgaaaaaact ggcattatac       660
ggcaaaatca atgacaacaa cagctggatt atcgataatg gcatttacca cattgcacct       720
ctgggtaaac tgcatagcaa caataaaatt ggtattgaga ccctgaccga agttatgaaa       780
gtttacccgt acctgagcat gcagcatctg caaagcgccg atcaaatcaa acgccactac       840
gatagcaagg atgccgaagg caacaagatc cctctggata aatttaaaaa agaaggtaaa       900
gaaaaatatt gtccgaaaac ctatacattt gatgatggca aagttattat taaggcaggc       960
gcacgcgtgg aagaggagaa agtgaaacgc ctgtattggg ccagcaaaga ggtgaacagt      1020
cagttcttcc gcgtttatgg catcgacaag ccgctggaag aaggcaaccc ggatgacatt      1080
ctgacgatgg tgatctataa cagcccggag gagtacaagc tgaatagtgt gttatatggt      1140
tatgatacca ataatggtgg tatgtatatc gagccggaag gcaccttctt cacctatgag      1200
cgtgaagccc aagagagtac ctataccctg gaggagctgt tccgtcacga gtatacacac      1260
tacctgcaag gccgctatgc agttccgggt caatggggcc gcaccaaact gtatgacaac      1320
gaccgtctga cctggtatga ggagggcggt gcagacttat ttgccggtag tacccgtacc      1380
agcggtattc tgccgcgtaa gagcatcgtt agcaacattc ataataccac acgtaacaat      1440
cgttacaaac tgagtgatac cgttcacagc aaatacggcg caagtttcga attttataat      1500
tacgcatgca tgtttatgga ctacatgtat aataaagata tgggcatcct gaataaactg      1560
aatgatctgg ccaagaataa tgatgttgac ggttacgata actacatccg cgatctgagc      1620
agcaattacg ccctgaatga taagtatcag gaccacatgc aggagcgcat cgacaattac      1680
gagaacttaa ccgtgccgtt cgttgccgac gactacctgg ttcgtcatgc ctataagaat      1740
ccgaatgaaa tctacagtga aattagcgag gttgcaaagc tgaaagacgc caagagcgag      1800
gtgaaaaaaa gtcagtattt cagtaccttc accttacgcg gcagttacac ggggggcgcc      1860
agcaagggta agctggaaga ccagaaagcc atgaataaat ttatcgatga tagcttaaaa      1920
aaattagata cctatagctg gagtggctac aaaaccctga ccgcatactt caccaactat      1980
aaggtggata gcagtaatcg cgtgacctat gacgtggtgt ttcacggcta cctgccgaat      2040
gagggtgata gcaagaacag cttaccgtac ggtaagatca acggcaccta caagggcacc      2100
gaaaaggaga agattaagtt cagcagtgaa ggcagcttcg accctgacgg caaaattgtg      2160
agttacgagt gggacttcgg cgatggcaac aagagcaacg aggagaaccc ggaacacagt      2220
tacgacaagg tgggcaccta cacagtgaaa ctgaaagtga ccgatgacaa aggcgaaagc      2280
agcgttagca ccacaaccgc agagatcaaa gacttaagcg agaataaact gccggtgatt      2340
tacatgcacg tgccgaaaag tggcgccctg aaccagaaag tggtgtttta tggcaaaggt      2400
acatacgacc cggatggcag catcgccggt tatcagtggg attttggcga cggcagtgat      2460
ttcagcagcg agcagaaccc gagtcatgtt tacaccaaga agggcgaata taccgtgacc      2520
ctgcgtgtga tggacagcag cggccagatg agtgaaaaaa ccatgaaaat caaaattacc      2580
gacccggtgt acccgattgg caccgagaaa gaaccgaaca acagcaagga gaccgccagc      2640
ggccctatcg ttcctggtat tcctgttagc ggcaccattg agaacacaag cgatcaggac      2700
tatttctatt ttgatgtgat caccccgggc gaagtgaaga ttgacattaa caaactgggt      2760
tatggtggcg ccacctgggt ggtgtacgat gagaacaaca atgccgtgag ttacgcaacc      2820
gacgatggcc agaatctgag cggcaaattc aaagccgaca agccgggtcg ctattacatt      2880
catctgtata tgttcaacgg cagctacatg ccgtatcgta ttaacattga aggtagcgtg      2940
ggtcgc                                                                 2946

<210>  2
<211>  982
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  2
Met Val Gln Asn Glu Ser Lys Arg Tyr Thr Val Ser Tyr Leu Lys Thr 
1               5                   10                  15      
Leu Asn Tyr Tyr Asp Leu Val Asp Leu Leu Val Lys Thr Glu Ile Glu 
            20                  25                  30          
Asn Leu Pro Asp Leu Phe Gln Tyr Ser Ser Asp Ala Lys Glu Phe Tyr 
        35                  40                  45              
Gly Asn Lys Thr Arg Met Ser Phe Ile Met Asp Glu Ile Gly Arg Arg 
    50                  55                  60                  
Ala Pro Gln Tyr Thr Glu Ile Asp His Lys Gly Ile Pro Thr Leu Val 
65                  70                  75                  80  
Glu Val Val Arg Ala Gly Phe Tyr Leu Gly Phe His Asn Lys Glu Leu 
                85                  90                  95      
Asn Glu Ile Asn Lys Arg Ser Phe Lys Glu Arg Val Ile Pro Ser Ile 
            100                 105                 110         
Leu Ala Ile Gln Lys Asn Pro Asn Phe Lys Leu Gly Thr Glu Val Gln 
        115                 120                 125             
Asp Lys Ile Val Ser Ala Thr Gly Leu Leu Ala Gly Asn Glu Thr Ala 
    130                 135                 140                 
Pro Pro Glu Val Val Asn Asn Phe Thr Pro Ile Leu Gln Asp Cys Ile 
145                 150                 155                 160 
Lys Asn Ile Asp Arg Tyr Ala Leu Asp Asp Leu Lys Ser Lys Ala Leu 
                165                 170                 175     
Phe Asn Val Leu Ala Ala Pro Thr Tyr Asp Ile Thr Glu Tyr Leu Arg 
            180                 185                 190         
Ala Thr Lys Glu Lys Pro Glu Asn Thr Pro Trp Tyr Gly Lys Ile Asp 
        195                 200                 205             
Gly Phe Ile Asn Glu Leu Lys Lys Leu Ala Leu Tyr Gly Lys Ile Asn 
    210                 215                 220                 
Asp Asn Asn Ser Trp Ile Ile Asp Asn Gly Ile Tyr His Ile Ala Pro 
225                 230                 235                 240 
Leu Gly Lys Leu His Ser Asn Asn Lys Ile Gly Ile Glu Thr Leu Thr 
                245                 250                 255     
Glu Val Met Lys Val Tyr Pro Tyr Leu Ser Met Gln His Leu Gln Ser 
            260                 265                 270         
Ala Asp Gln Ile Lys Arg His Tyr Asp Ser Lys Asp Ala Glu Gly Asn 
        275                 280                 285             
Lys Ile Pro Leu Asp Lys Phe Lys Lys Glu Gly Lys Glu Lys Tyr Cys 
    290                 295                 300                 
Pro Lys Thr Tyr Thr Phe Asp Asp Gly Lys Val Ile Ile Lys Ala Gly 
305                 310                 315                 320 
Ala Arg Val Glu Glu Glu Lys Val Lys Arg Leu Tyr Trp Ala Ser Lys 
                325                 330                 335     
Glu Val Asn Ser Gln Phe Phe Arg Val Tyr Gly Ile Asp Lys Pro Leu 
            340                 345                 350         
Glu Glu Gly Asn Pro Asp Asp Ile Leu Thr Met Val Ile Tyr Asn Ser 
        355                 360                 365             
Pro Glu Glu Tyr Lys Leu Asn Ser Val Leu Tyr Gly Tyr Asp Thr Asn 
    370                 375                 380                 
Asn Gly Gly Met Tyr Ile Glu Pro Glu Gly Thr Phe Phe Thr Tyr Glu 
385                 390                 395                 400 
Arg Glu Ala Gln Glu Ser Thr Tyr Thr Leu Glu Glu Leu Phe Arg His 
                405                 410                 415     
Glu Tyr Thr His Tyr Leu Gln Gly Arg Tyr Ala Val Pro Gly Gln Trp 
            420                 425                 430         
Gly Arg Thr Lys Leu Tyr Asp Asn Asp Arg Leu Thr Trp Tyr Glu Glu 
        435                 440                 445             
Gly Gly Ala Asp Leu Phe Ala Gly Ser Thr Arg Thr Ser Gly Ile Leu 
    450                 455                 460                 
Pro Arg Lys Ser Ile Val Ser Asn Ile His Asn Thr Thr Arg Asn Asn 
465                 470                 475                 480 
Arg Tyr Lys Leu Ser Asp Thr Val His Ser Lys Tyr Gly Ala Ser Phe 
                485                 490                 495     
Glu Phe Tyr Asn Tyr Ala Cys Met Phe Met Asp Tyr Met Tyr Asn Lys 
            500                 505                 510         
Asp Met Gly Ile Leu Asn Lys Leu Asn Asp Leu Ala Lys Asn Asn Asp 
        515                 520                 525             
Val Asp Gly Tyr Asp Asn Tyr Ile Arg Asp Leu Ser Ser Asn Tyr Ala 
    530                 535                 540                 
Leu Asn Asp Lys Tyr Gln Asp His Met Gln Glu Arg Ile Asp Asn Tyr 
545                 550                 555                 560 
Glu Asn Leu Thr Val Pro Phe Val Ala Asp Asp Tyr Leu Val Arg His 
                565                 570                 575     
Ala Tyr Lys Asn Pro Asn Glu Ile Tyr Ser Glu Ile Ser Glu Val Ala 
            580                 585                 590         
Lys Leu Lys Asp Ala Lys Ser Glu Val Lys Lys Ser Gln Tyr Phe Ser 
        595                 600                 605             
Thr Phe Thr Leu Arg Gly Ser Tyr Thr Gly Gly Ala Ser Lys Gly Lys 
    610                 615                 620                 
Leu Glu Asp Gln Lys Ala Met Asn Lys Phe Ile Asp Asp Ser Leu Lys 
625                 630                 635                 640 
Lys Leu Asp Thr Tyr Ser Trp Ser Gly Tyr Lys Thr Leu Thr Ala Tyr 
                645                 650                 655     
Phe Thr Asn Tyr Lys Val Asp Ser Ser Asn Arg Val Thr Tyr Asp Val 
            660                 665                 670         
Val Phe His Gly Tyr Leu Pro Asn Glu Gly Asp Ser Lys Asn Ser Leu 
        675                 680                 685             
Pro Tyr Gly Lys Ile Asn Gly Thr Tyr Lys Gly Thr Glu Lys Glu Lys 
    690                 695                 700                 
Ile Lys Phe Ser Ser Glu Gly Ser Phe Asp Pro Asp Gly Lys Ile Val 
705                 710                 715                 720 
Ser Tyr Glu Trp Asp Phe Gly Asp Gly Asn Lys Ser Asn Glu Glu Asn 
                725                 730                 735     
Pro Glu His Ser Tyr Asp Lys Val Gly Thr Tyr Thr Val Lys Leu Lys 
            740                 745                 750         
Val Thr Asp Asp Lys Gly Glu Ser Ser Val Ser Thr Thr Thr Ala Glu 
        755                 760                 765             
Ile Lys Asp Leu Ser Glu Asn Lys Leu Pro Val Ile Tyr Met His Val 
    770                 775                 780                 
Pro Lys Ser Gly Ala Leu Asn Gln Lys Val Val Phe Tyr Gly Lys Gly 
785                 790                 795                 800 
Thr Tyr Asp Pro Asp Gly Ser Ile Ala Gly Tyr Gln Trp Asp Phe Gly 
                805                 810                 815     
Asp Gly Ser Asp Phe Ser Ser Glu Gln Asn Pro Ser His Val Tyr Thr 
            820                 825                 830         
Lys Lys Gly Glu Tyr Thr Val Thr Leu Arg Val Met Asp Ser Ser Gly 
        835                 840                 845             
Gln Met Ser Glu Lys Thr Met Lys Ile Lys Ile Thr Asp Pro Val Tyr 
    850                 855                 860                 
Pro Ile Gly Thr Glu Lys Glu Pro Asn Asn Ser Lys Glu Thr Ala Ser 
865                 870                 875                 880 
Gly Pro Ile Val Pro Gly Ile Pro Val Ser Gly Thr Ile Glu Asn Thr 
                885                 890                 895     
Ser Asp Gln Asp Tyr Phe Tyr Phe Asp Val Ile Thr Pro Gly Glu Val 
            900                 905                 910         
Lys Ile Asp Ile Asn Lys Leu Gly Tyr Gly Gly Ala Thr Trp Val Val 
        915                 920                 925             
Tyr Asp Glu Asn Asn Asn Ala Val Ser Tyr Ala Thr Asp Asp Gly Gln 
    930                 935                 940                 
Asn Leu Ser Gly Lys Phe Lys Ala Asp Lys Pro Gly Arg Tyr Tyr Ile 
945                 950                 955                 960 
His Leu Tyr Met Phe Asn Gly Ser Tyr Met Pro Tyr Arg Ile Asn Ile 
                965                 970                 975     
Glu Gly Ser Val Gly Arg 
            980         

