﻿序列表
<110>  马哲

<120>  一种用于检测新型冠状病毒抗体的无创取样检测试剂盒及其检测方法

<160>  11

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  1273
<212>  PRT
<213>  surface glycoprotein

<220>
<221>  UNSURE
<222>  (1)..(1273)

<400>  1
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 
Glu Cys Val Leu Gly Gln Ser Lys Arg Val Asp Phe Cys Gly Lys Gly 
                165                 170                 175     
Tyr His Leu Met Ser Phe Pro Gln Ser Ala Pro His Gly Val Val Phe 
            180                 185                 190         
Leu His Val Thr Tyr Val Pro Ala Gln Glu Lys Asn Phe Thr Thr Ala 
        195                 200                 205             
Pro Ala Ile Cys His Asp Gly Lys Ala His Phe Pro Arg Glu Gly Val 
    210                 215                 220                 
Phe Val Ser Asn Gly Thr His Trp Phe Val Thr Gln Arg Asn Phe Tyr 
225                 230                 235                 240 
Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr Phe Val Ser Gly Asn Cys 
                245                 250                 255     
Asp Val Val Ile Gly Ile Val Asn Asn Thr Val Tyr Asp Pro Leu Gln 
            260                 265                 270         
Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu Asp Lys Tyr Phe Lys Asn 
        275                 280                 285             
His Thr Ser Pro Asp Val Asp Leu Gly Asp Ile Ser Gly Ile Asn Ala 
    290                 295                 300                 
Ser Val Val Asn Ile Gln Lys Glu Ile Asp Arg Leu Asn Glu Val Ala 
305                 310                 315                 320 
Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu Gln Glu Leu Gly Ser Ser 
                325                 330                 335     
Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu Met Asp 
            340                 345                 350         
Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe Val Phe 
        355                 360                 365             
Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr Pro Ile 
    370                 375                 380                 
Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu Pro Leu 
385                 390                 395                 400 
Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr Leu Leu 
                405                 410                 415     
Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser Gly Trp 
            420                 425                 430         
Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro Arg Thr 
        435                 440                 445             
Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala Val Asp 
    450                 455                 460                 
Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys Ser Phe 
465                 470                 475                 480 
Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val Gln Pro 
                485                 490                 495     
Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe 
            500                 505                 510         
Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn 
        515                 520                 525             
Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn 
    530                 535                 540                 
Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys 
545                 550                 555                 560 
Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile 
                565                 570                 575     
Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile 
            580                 585                 590         
Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile 
        595                 600                 605             
Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn 
    610                 615                 620                 
Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg 
625                 630                 635                 640 
Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly 
                645                 650                 655     
Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln 
            660                 665                 670         
Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser 
        675                 680                 685             
Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser 
    690                 695                 700                 
Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn Gly Leu 
705                 710                 715                 720 
Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu Pro Phe 
                725                 730                 735     
Gln Gln Phe Gly Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     
Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         
Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             
Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn Leu 
    1010                 1015                 1020                 
Ala  Ala Thr Lys Met Ser  Arg Asp Ile Ala Asp  Thr Thr Asp Ala Val  
1025                 1030                 1035                 1040 
Arg Asp Pro Gln Thr  Leu Glu Ile Leu Asp  Ile Thr Pro Cys Ser  Phe 
                1045                 1050                 1055     
Gly Gly Val Ser  Val Ile Thr Pro Gly  Thr Asn Thr Ser Asn  Gln Val 
            1060                 1065                 1070         
Ala Val Leu  Tyr Gln Asp Val Asn  Cys Thr Glu Val Pro  Val Ala Ile 
        1075                 1080                 1085             
His Ala  Asp Gln Leu Thr Pro  Thr Trp Arg Val Tyr  Ser Thr Gly Ser 
    1090                 1095                 1100                 
Asn  Val Phe Gln Thr Arg  Ala Gly Cys Leu Ile  Gly Ala Glu His Val  
1105                 1110                 1115                 1120 
Asn Asn Ser Tyr Glu  Cys Asp Ile Pro Ile  Gly Ala Gly Ile Cys  Ala 
                1125                 1130                 1135     
Ser Tyr Gln Thr  Gln Thr Asn Ser Pro  Arg Arg Ala Arg Ser  Val Ala 
            1140                 1145                 1150         
Ser Gln Ser  Ile Ile Ala Tyr Thr  Met Ser Leu Gly Ala  Glu Asn Ser 
        1155                 1160                 1165             
Val Ala  Tyr Ser Asn Asn Ser  Ile Ala Ile Pro Thr  Asn Phe Thr Ile 
    1170                 1175                 1180                 
Ser  Val Thr Thr Glu Ile  Leu Pro Val Ser Met  Thr Lys Thr Ser Val  
1185                 1190                 1195                 1200 
Asp Cys Thr Met Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile 
                1205                 1210                 1215     
Trp Leu Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile 
            1220                 1225                 1230         
Met Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
        1235                 1240                 1245             
Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro Val 
    1250                 1255                 1260                 
Leu  Lys Gly Val Lys Leu  His Tyr Thr 
1265                 1270             

<210>  2
<211>  275
<212>  PRT
<213>  β-CoV S-protein

<220>
<221>  UNSURE
<222>  (1)..(275)

<400>  2
Met Asp Leu Phe Met Arg Ile Phe Thr Ile Gly Thr Val Thr Leu Lys 
1               5                   10                  15      
Gln Gly Glu Ile Lys Asp Ala Thr Pro Ser Asp Phe Val Arg Ala Thr 
            20                  25                  30          
Ala Thr Ile Pro Ile Gln Ala Ser Leu Pro Phe Gly Ala Pro Phe Leu 
        35                  40                  45              
Tyr Leu Tyr Ala Leu Val Tyr Phe Leu Gln Ser Ile Asn Phe Val Arg 
    50                  55                  60                  
Ile Ile Met Arg Leu Trp Leu Cys Trp Lys Cys Arg Ser Lys Asn Pro 
65                  70                  75                  80  
Leu Leu Tyr Asp Ala Asn Tyr Phe Leu Cys Trp His Thr Asn Cys Tyr 
                85                  90                  95      
Asp Tyr Cys Ile Pro Tyr Trp Leu Ile Val Gly Val Ala Leu Leu Ala 
            100                 105                 110         
Val Phe Gln Ser Ala Ser Lys Ile Ile Thr Leu Lys Lys Arg Trp Gln 
        115                 120                 125             
Leu Ala Leu Ser Lys Gly Val His Phe Val Cys Asn Leu Leu Leu Leu 
    130                 135                 140                 
Phe Val Thr Val Tyr Ser His Leu Leu Leu Val Ala Ala Gly Leu Glu 
145                 150                 155                 160 
Asn Ser Val Thr Ser Ser Ile Val Ile Thr Ser Gly Asp Gly Thr Thr 
                165                 170                 175     
Ser Pro Ile Ser Glu His Asp Tyr Gln Ile Gly Gly Tyr Thr Glu Lys 
            180                 185                 190         
Trp Glu Ser Gly Val Lys Asp Cys Val Val Leu His Ser Tyr Phe Thr 
        195                 200                 205             
Ser Asp Tyr Tyr Gln Leu Tyr Ser Thr Gln Leu Ser Thr Asp Thr Gly 
    210                 215                 220                 
Val Glu His Val Thr Phe Phe Ile Tyr Asn Lys Ile Val Asp Glu Pro 
225                 230                 235                 240 
Glu Glu His Val Gln Ile His Thr Ile Asp Gly Ser Ser Gly Val Val 
                245                 250                 255     
Asn Pro Val Met Glu Pro Ile Tyr Asp Glu Pro Thr Thr Thr Thr Ser 
            260                 265                 270         
Val Pro Leu 
        275 

<210>  3
<211>  75
<212>  PRT
<213>  E protein

<220>
<221>  UNSURE
<222>  (1)..(75)

<400>  3
Met Tyr Ser Phe Val Ser Glu Glu Thr Gly Thr Leu Ile Val Asn Ser 
1               5                   10                  15      
Val Leu Leu Phe Leu Ala Phe Val Val Phe Leu Leu Val Thr Leu Ala 
            20                  25                  30          
Ile Leu Thr Ala Leu Arg Leu Cys Ala Tyr Cys Cys Asn Ile Val Asn 
        35                  40                  45              
Val Ser Leu Val Lys Pro Ser Phe Tyr Val Tyr Ser Arg Val Lys Asn 
    50                  55                  60                  
Leu Asn Ser Ser Arg Val Pro Asp Leu Leu Val 
65                  70                  75  

<210>  4
<211>  218
<212>  PRT
<213>  membrane glycoprotein

<220>
<221>  UNSURE
<222>  (1)..(218)

<400>  4
Met Ala Asp Ser Asn Gly Thr Ile Thr Val Glu Glu Leu Lys Lys Leu 
1               5                   10                  15      
Leu Glu Gln Trp Asn Leu Val Ile Gly Phe Leu Phe Leu Thr Trp Ile 
            20                  25                  30          
Cys Leu Leu Gln Phe Ala Tyr Ala Asn Arg Asn Arg Phe Ala Arg Thr 
        35                  40                  45              
Arg Ser Met Trp Ser Phe Asn Pro Glu Thr Asn Ile Leu Leu Asn Val 
    50                  55                  60                  
Pro Leu His Gly Thr Ile Leu Thr Arg Pro Leu Leu Glu Ser Glu Leu 
65                  70                  75                  80  
Val Ile Gly Ala Val Ile Leu Arg Gly His Leu Arg Ile Ala Gly His 
                85                  90                  95      
His Leu Gly Arg Cys Asp Ile Lys Asp Leu Pro Lys Glu Ile Thr Val 
            100                 105                 110         
Ala Thr Ser Arg Thr Leu Ser Tyr Tyr Lys Leu Gly Ala Ser Gln Arg 
        115                 120                 125             
Val Ala Gly Asp Ser Gly Phe Ala Ala Tyr Ser Arg Tyr Arg Ile Gly 
    130                 135                 140                 
Asn Tyr Lys Leu Asn Thr Asp His Ser Ser Ser Ser Asp Asn Ile Ala 
145                 150                 155                 160 
Phe Leu Tyr Ile Ile Lys Leu Ile Phe Leu Trp Leu Leu Trp Pro Val 
                165                 170                 175     
Thr Leu Ala Cys Phe Val Leu Ala Ala Val Tyr Arg Ile Asn Trp Ile 
            180                 185                 190         
Thr Gly Gly Ile Ala Ile Ala Met Ala Cys Leu Val Gly Leu Met Trp 
        195                 200                 205             
Leu Ser Tyr Phe Ile Ala Ser Phe Arg Leu 
    210                 215             

<210>  5
<211>  61
<212>  PRT
<213>  ACE-2 (with C-terminal human Fc tag)

<220>
<221>  UNSURE
<222>  (1)..(61)

<400>  5
Met Phe His Leu Val Asp Phe Gln Val Thr Ile Ala Glu Ile Leu Leu 
1               5                   10                  15      
Ile Ile Met Arg Thr Phe Lys Val Ser Ile Trp Asn Leu Asp Tyr Ile 
            20                  25                  30          
Ile Asn Leu Ile Ile Lys Asn Leu Ser Lys Ser Leu Thr Glu Asn Lys 
        35                  40                  45              
Tyr Ser Gln Leu Asp Glu Glu Gln Pro Met Glu Ile Asp 
    50                  55                  60      

<210>  6
<211>  121
<212>  PRT
<213>  receptor-binding domain protein

<220>
<221>  UNSURE
<222>  (1)..(121)

<400>  6
Met Lys Ile Ile Leu Phe Leu Ala Leu Ile Thr Leu Ala Thr Cys Glu 
1               5                   10                  15      
Leu Tyr His Tyr Gln Glu Cys Val Arg Gly Thr Thr Val Leu Leu Lys 
            20                  25                  30          
Glu Pro Cys Ser Ser Gly Thr Tyr Glu Gly Asn Ser Pro Phe His Pro 
        35                  40                  45              
Leu Ala Asp Asn Lys Phe Ala Leu Thr Cys Phe Ser Thr Gln Phe Ala 
    50                  55                  60                  
Phe Ala Cys Pro Asp Gly Val Lys His Val Tyr Gln Leu Arg Ala Arg 
65                  70                  75                  80  
Ser Val Ser Pro Lys Leu Phe Ile Arg Gln Glu Glu Val Gln Glu Leu 
                85                  90                  95      
Tyr Ser Pro Ile Phe Leu Ile Val Ala Ala Ile Val Phe Ile Thr Leu 
            100                 105                 110         
Cys Phe Thr Leu Lys Arg Lys Thr Glu 
        115                 120     

<210>  7
<211>  43
<212>  PRT
<213>  N-terminal domain (NTD) protein

<220>
<221>  UNSURE
<222>  (1)..(43)

<400>  7
Met Ile Glu Leu Ser Leu Ile Asp Phe Tyr Leu Cys Phe Leu Ala Phe 
1               5                   10                  15      
Leu Leu Phe Leu Val Leu Ile Met Leu Ile Ile Phe Trp Phe Ser Leu 
            20                  25                  30          
Glu Leu Gln Asp His Asn Glu Thr Cys His Ala 
        35                  40              

<210>  8
<211>  121
<212>  PRT
<213>  ORF8 protein,cryo-EM structures

<220>
<221>  UNSURE
<222>  (1)..(121)

<400>  8
Met Lys Phe Leu Val Phe Leu Gly Ile Ile Thr Thr Val Ala Ala Phe 
1               5                   10                  15      
His Gln Glu Cys Ser Leu Gln Ser Cys Thr Gln His Gln Pro Tyr Val 
            20                  25                  30          
Val Asp Asp Pro Cys Pro Ile His Phe Tyr Ser Lys Trp Tyr Ile Arg 
        35                  40                  45              
Val Gly Ala Arg Lys Ser Ala Pro Leu Ile Glu Leu Cys Val Asp Glu 
    50                  55                  60                  
Ala Gly Ser Lys Ser Pro Ile Gln Tyr Ile Asp Ile Gly Asn Tyr Thr 
65                  70                  75                  80  
Val Ser Cys Leu Pro Phe Thr Ile Asn Cys Gln Glu Pro Lys Leu Gly 
                85                  90                  95      
Ser Leu Val Val Arg Cys Ser Phe Tyr Glu Asp Phe Leu Glu Tyr His 
            100                 105                 110         
Asp Val Arg Val Val Leu Asp Phe Ile 
        115                 120     

<210>  9
<211>  419
<212>  PRT
<213>  nucleocapsid phosphoprotein

<220>
<221>  UNSURE
<222>  (1)..(419)

<400>  9
Met Ser Asp Asn Gly Pro Gln Asn Gln Arg Asn Ala Pro Arg Ile Thr 
1               5                   10                  15      
Phe Gly Gly Pro Ser Asp Ser Thr Gly Ser Asn Gln Asn Gly Glu Arg 
            20                  25                  30          
Ser Gly Ala Arg Ser Lys Gln Arg Arg Pro Gln Gly Leu Pro Asn Asn 
        35                  40                  45              
Thr Ala Ser Trp Phe Thr Ala Leu Thr Gln His Gly Lys Glu Asp Leu 
    50                  55                  60                  
Lys Phe Pro Arg Gly Gln Gly Val Pro Ile Asn Thr Asn Ser Ser Pro 
65                  70                  75                  80  
Asp Asp Gln Ile Gly Tyr Tyr Arg Arg Ala Thr Arg Arg Ile Arg Gly 
                85                  90                  95      
Gly Asp Gly Lys Met Lys Leu Pro Gln Gly Thr Thr Leu Pro Lys Gly 
            100                 105                 110         
Phe Tyr Ala Glu Gly Ser Arg Gly Gly Ser Gln Ala Ser Ser Arg Ser 
        115                 120                 125             
Ser Ser Arg Ser Arg Asn Ser Ser Arg Asn Ser Thr Pro Gly Ser Ser 
    130                 135                 140                 
Arg Gly Thr Ser Pro Ala Arg Met Ala Gly Asn Gly Gly Asp Ala Ala 
145                 150                 155                 160 
Leu Ala Leu Leu Leu Leu Asp Arg Leu Asn Gln Leu Glu Ser Lys Met 
                165                 170                 175     
Ser Gly Lys Gly Gln Gln Gln Gln Gly Gln Thr Val Thr Lys Lys Ser 
            180                 185                 190         
Ala Ala Glu Ala Ser Lys Lys Pro Arg Gln Lys Arg Thr Ala Thr Lys 
        195                 200                 205             
Ala Tyr Asn Val Thr Gln Ala Phe Gly Arg Arg Gly Pro Glu Gln Thr 
    210                 215                 220                 
Gln Gly Asn Phe Gly Asp Gln Glu Leu Ile Arg Gln Gly Thr Asp Tyr 
225                 230                 235                 240 
Lys His Trp Pro Gln Ile Ala Gln Phe Ala Pro Ser Ala Ser Ala Phe 
                245                 250                 255     
Phe Gly Met Ser Arg Ile Gly Met Glu Val Thr Pro Ser Gly Thr Trp 
            260                 265                 270         
Leu Thr Tyr Thr Asp Leu Ser Pro Arg Trp Tyr Phe Tyr Tyr Leu Gly 
        275                 280                 285             
Thr Gly Pro Glu Ala Gly Leu Pro Tyr Gly Ala Asn Lys Asp Gly Ile 
    290                 295                 300                 
Ile Trp Val Ala Thr Glu Gly Ala Leu Asn Thr Pro Lys Asp His Ile 
305                 310                 315                 320 
Gly Thr Arg Asn Pro Ala Asn Asn Ala Ala Ile Val Leu Gln Gly Ala 
                325                 330                 335     
Ile Lys Leu Asp Asp Lys Asp Pro Asn Phe Lys Asp Gln Val Ile Leu 
            340                 345                 350         
Leu Asn Lys His Ile Asp Ala Tyr Lys Thr Phe Pro Pro Thr Glu Pro 
        355                 360                 365             
Lys Lys Asp Lys Lys Lys Lys Ala Asp Glu Thr Gln Ala Leu Pro Gln 
    370                 375                 380                 
Arg Gln Lys Lys Gln Gln Thr Val Thr Leu Leu Pro Ala Ala Asp Leu 
385                 390                 395                 400 
Asp Asp Phe Ser Lys Gln Leu Gln Gln Ser Met Ser Ser Ala Asp Ser 
                405                 410                 415     
Thr Gln Ala 
            

<210>  10
<211>  38
<212>  PRT
<213>  structurally conserved subdomains 2

<220>
<221>  UNSURE
<222>  (1)..(38)

<400>  10
Met Gly Tyr Ile Asn Val Phe Ala Phe Pro Phe Thr Ile Tyr Ser Leu 
1               5                   10                  15      
Leu Leu Cys Arg Met Asn Ser Arg Asn Tyr Ile Ala Gln Val Asp Val 
            20                  25                  30          
Val Asn Phe Asn Leu Thr 
        35              

<210>  11
<211>  8723
<212>  DNA
<213>  Coronavirus 3' stem-loop II-like motif (s2m)

<220>
<221>  unsure
<222>  (1)..(8723)

<400>  11
attaaaggtt tataccttcc caggtaacaa accaaccaac tttcgatctc ttgtagatct        60
gttctctaaa cgaactttaa aatctgtgtg gctgtcactc ggctgcatgc ttagtgcact       120
cacgcagtat aattaataac taattactgt cgttgacagg acacgagtaa ctcgtctatc       180
ttctgcaggc tgcttacggt ttcgtccgtg ttgcagccga tcatcagcac atctaggttt       240
cgtccgggtg tgaccgaaag gtatgtttgt ttttcttgtt ttattgccac tagtctctag       300
tcagtgtgtt aatcttacaa ccagaactca attaccccct gcatacacta attctttcac       360
acgtggtgtt tattaccctg acaaagtttt cagatcctca gttttacatt caactcagga       420
cttgttctta cctttctttt ccaatgttac ttggttccat gctatacatg tctctgggac       480
caatggtact aagaggtttg ataaccctgt cctaccattt aatgatggtg tttattttgc       540
ttccactgag aagtctaaca taataagagg ctggattttt ggtactactt tagattcgaa       600
gacccagtcc ctacttattg ttaataacgc tactaatgtt gttattaaag tctgtgaatt       660
ttcttcttca ggttggacag ctggtgctgc agcttattat gtgggttatc ttcaacctag       720
gacttttcta ttaaaatata atgaaaatgg aaccattaca gatgctgtag actgtgcact       780
tgaccctctc tcagaaacaa agtgtacgtt gaaatccttc actgtagaaa aaggaatcta       840
tcaaacttct aactttagag tccaaccaac agaatctatt gttagatttc ctaatattac       900
aaacttgtgc ccttttggtg aagtttttaa cgccaccaga tttgcatctg tttatgcttg       960
gaacaggaag agaatcagca actgtgttgc tgattattct gtcctatata attccgcatc      1020
tcaattttgt aatgatccat ttttgggtgt ttattaccac aaaaacaaca aaagttggat      1080
ggaaagtgag ttcagagttt attctagtgc gaataattgc acttttgaat atgtctctca      1140
gccttttctt atggaccttg aaggaaaaca gggtaatttc aaaaatctta gggaatttgt      1200
gtttaagaat attgatggtt attttaaaat atattctaag cacacgccta ttaatttagt      1260
gcgtgatctc cctcagggtt tttcggcttt agaaccattg gtagatttgc caataggtat      1320
taacatcact aggtttcaaa ctttacttgc tttacataga agttatttga ctcctggtga      1380
attttccact tttaagtgtt atggagtgtc tcctactaaa ttaaatgatc tctgctttac      1440
taatgtctat gcagattcat ttgtaattag aggtgatgaa gtcagacaaa tcgctccagg      1500
gcaaactgga aagattgctg attataatta taaattacca gatgatttta caggctgcgt      1560
tatagcttgg aattctaaca atcttgattc taaggttggt ggtaattata attacctgta      1620
tgatgctgtc cgtgatccac agacacttga gattcttgac attacaccat gttcttttgg      1680
tggtgtcagt gttataacac caggaacaaa tacttctaac caggttgctg ttctttatca      1740
ggatgttaac tgcacagaag tccctgttgc tattcatgca gatcaactta ctcctacttg      1800
gcgtgtttat tctacaggtt ctaatgtttt tcaaacacgt gcaggctgtt taataggggc      1860
tgaacatgtc aacaactcat atgagtgtga catacccatt ggtgcaggta tatgcgctag      1920
ttatcagact cagactaatt ctcctcggcg ggcacgtagt gtagctagtc aatccatcat      1980
tgcctacact atgtcacttg gtgcagaaaa ttcagttgct tactctaata actctattgc      2040
catacccaca aattttacta ttagtgttac cacagaaatt ctaccagtgt ctatgaccaa      2100
tagattgttt aggaagtcta atctcaaacc ttttgagaga gatatttcaa ctgaaatcta      2160
tcaggccggt agcacacctt gtaatggtgt tgaaggtttt aattgttact ttcctttaca      2220
atcatatggt ttccaaccca ctaatggtgt tggttaccaa ccatacagag tagtagtact      2280
ttcttttgaa cttctacatg caccagcaac tgtttgtgga cctaaaaagt ctactaattt      2340
ggttaaaaac aaatgtgtca atttcaactt caatggttta acaggcacag gtgttcttac      2400
tgagtctaac aaaaagtttc tgcctttcca acaatttggc agagacattg ctgacactac      2460
gacatcagta gattgtacaa tgtacatttg tggtgattca actgaatgca gcaatctttt      2520
gttgcaatat ggcagttttt gtacacaatt aaaccgtgct ttaactggaa tagctgttga      2580
acaagacaaa aacacccaag aagtttttgc acaagtcaaa caaatttaca aaacaccacc      2640
aattaaagat tttggtggtt ttaatttttc acaaatatta ccagatccat caaaaccaag      2700
caagaggtca tttattgaag atctactttt caacaaagtg acacttgcag atgctggctt      2760
catcaaacaa tatggtgatt gccttggtga tattgctgct agagacctca tttgtgcaca      2820
aaagtttaac ggccttactg ttttgccacc tttgctcaca gatgaaatga ttgctcaata      2880
cacttctgca ctgttagcgg gtacaatcac ttctggttgg acctttggtg caggtgctgc      2940
aattcaagac tcactttctt ccacagcaag tgcacttgga aaacttcaag atgtggtcaa      3000
ccaaaatgca caagctttaa acacgcttgt taaacaactt agctccaatt ttggtgcaat      3060
ttcaagtgtt ttaaatgata tcctttcacg tcttgacaaa gttgaggctg aagtgcaaat      3120
tgataggttg atcacaggca gacttcaaag tttgcagaca tatgtgactc aacaattaat      3180
tagagctgca gaaatcagag cttctgctaa tcttgctgct actaaaatgt cagagtgtgt      3240
acttggacaa tcaaaaagag ttgatttttg tggaaagggc tatcatctta tgtccttccc      3300
attacaaata ccatttgcta tgcaaatggc ttataggttt aatggtattg gagttacaca      3360
gaatgttctc tatgagaacc aaaaattgat tgccaaccaa tttaatagtg ctattggcaa      3420
tcagtcagca cctcatggtg tagtcttctt gcatgtgact tatgtccctg cacaagaaaa      3480
gaacttcaca actgctcctg ccatttgtca tgatggaaaa gcacactttc ctcgtgaagg      3540
tgtctttgtt tcaaatggca cacactggtt tgtaacacaa aggaattttt atgaaccaca      3600
aatcattact acagacaaca catttgtgtc tggtaactgt gatgttgtaa taggaattgt      3660
caacaacaca gtttatgatc ctttgcaacc tgaattagac tcattcaagg aggagttaga      3720
taaatatttt aagaatcata catcaccaga tgttgattta ggtgacatct ctggcattaa      3780
tgcttcagtt gtaaacattc aaaaagaaat tgaccgcctc aatgaggttg ccaagaattt      3840
aaatgaatct ctcatcgatc tccaagaact tggaaagtat gagcagtata taaaatggcc      3900
atggtacatt tggctaggtt ttatagctgg cttgattgcc atagtaatgg tgacaattat      3960
gctttgctgt atgaccagtt gctgtagttg tctcaagggc tgttgttctt gtggatcctg      4020
ctgcaaattt gatgaagacg actctgagcc agtgctcaaa ggagtcaaat tacattacac      4080
ataaacgaac ttatggattt gtttatgaga atcttcacaa ttggaactgt aactttgaag      4140
caaggtgaaa tcaaggatgc tactccttca gattttgttc gcgctactgc aacgataccg      4200
atacaagcct cactcccttt cggatggctt attgttggcg ttgcacttct tgctgttttt      4260
cagagcgctt ccaaaatcat aaccctcaaa aagagatggc aactagcact ctccaagggt      4320
gttcactttg tttgcaactt gctgttgttg tttgtaacag tttactcaca ccttttgctc      4380
gttgctgctg gccttgaagc cccttttctc tatctttatg ctttagtcta cttcttgcag      4440
agtataaact ttgtaagaat aataatgagg ctttggcttt gctggaaatg ccgttccaaa      4500
aacccattac tttatgatgc caactatttt ctttgctggc atactaattg ttacgactat      4560
tgtatacctt acaatagtgt aacttcttca attgtcatta cttcaggtga tggcacaaca      4620
agtcctattt ctgaacatga ctaccagatt ggtggttata ctgaaaaatg ggaatctgga      4680
gtaaaagact gtgttgtatt acacagttac ttcacttcag actattacca gctgtactca      4740
actcaattga gtacagacac tggtgttgaa catgttacct tcttcatcta caataaaatt      4800
gttgatgagc ctgaagaaca tgtccaaatt cacacaatcg acggttcatc cggagttgtt      4860
aatccagtaa tggaaccaat ttatgatgaa ccgacgacga ctactagcgt gcctttgtaa      4920
gcacaagctg atgagtacga acttatgtac tcattcgttt cggaagagac aggtacgtta      4980
atagttaata gcgtacttct ttttcttgct ttcgtggtat tcttgctagt tacactagcc      5040
atccttactg cgcttcgatt gtgtgcgtac tgctgcaata ttgttaacgt gagtcttgta      5100
aaaccttctt tttacgttta ctctcgtgtt aaaaatctga attcttctag agttcctgat      5160
ggattggcaa ctataaatta aacacagacc attccagtag cagtgacaat attgctttgc      5220
ttgtacagta agtgacaaca gatgtttcat ctcgttgact ttcaggttac tatagcagag      5280
atattactaa ttattatgag gacttttaaa gtttccattt ggaatcttga ttacatcata      5340
aacctcataa ttaaaaattt atctaagtca ctaactgaga ataaatattc tcaattagat      5400
gaagagcaac caatggagat tgattaaacg aacatgaaaa ttattctttt cttggcactg      5460
ataacactcg ctacttgtga gctttatcac taccaagagt gtgttagagg tacaacagta      5520
cttttaaaag aaccttgctc ttctggaaca tacgagggca attcaccatt tcatcctcta      5580
gctgataaca aatttgcact gacttgcttt agcactcaat ttgcttttgc ttgtcctgac      5640
ggcgtaaaac acgtctatca gttacgtgcc agatcagttt cacctaaact gttcatcaga      5700
caagaggaag ttcaagaact ttactctcca atttttctta ttgttgcggc aatagtgttt      5760
ataacacttt gcttcacact caaaagaaag acagaatgat tgaactttca ttaattgact      5820
tctatttgtg ctttttagcc tttctgctat tccttgtttt aattatgctt attatctttt      5880
ggttctcact tgaactgcaa gatcataatg aaacttgtca cgcctaaacg aacatgaaat      5940
ttcttgtttt cttaggaatc atcacaactg tagctgcatt tcaccaagaa tgtagtttac      6000
agtcatgtac tcaacatcaa ccatatgtag ttgatgaccc gtgtcctatt cacttctatt      6060
ctaaatggta tattagagta ggagctagaa aatcagcacc tttaattgaa ttgtgcgtgg      6120
atgaggctgg ttctaaatca cccattcagt acatcgatat cggtaattat acagtttcct      6180
gtttaccttt tacaattaat tgccaggaac ctaaattggg tagtcttgta gtgcgttgtt      6240
cgttctatga agacttttta gagtatcatg acgttcgtgt tgttttagat ttcatctaaa      6300
cgaacaaact aaaatgtctg ataatggacc ccaaaatcag cgaaatgcac cccgcattac      6360
gtttggtgga ccctcagatt caactggcag taaccagaat ggagaacgca gtggggcgcg      6420
cttctggtct aaacgaacta aatattatat tagtttttct gtttggaact ttaattttag      6480
ccatggcaga ttccaacggt actattaccg ttgaagagct taaaaagctc cttgaacaat      6540
ggaacctagt aataggtttc ctattcctta catggatttg tcttctacaa tttgcctatg      6600
ccaacaggaa taggtttttg tatataatta agttaatttt cctctggctg ttatggccag      6660
taactttagc ttgttttgtg cttgctgctg tttacagaat aaattggatc accggtggaa      6720
ttgctatcgc aatggcttgt cttgtaggct tgatgtggct cagctacttc attgcttctt      6780
tcagactgtt tgcgcgtacg cgttccatgt ggtcattcaa tccagaaact aacattcttc      6840
tcaacgtgcc actccatggc actattctga ccagaccgct tctagaaagt gaactcgtaa      6900
tcggagctgt gatccttcgt ggacatcttc gtattgctgg acaccatcta ggacgctgtg      6960
acatcaagga cctgcctaaa gaaatcactg ttgctacatc acgaacgctt tcttattaca      7020
tggtgacggt aaaatgaaag atctcagtcc aagatggtat ttctactacc taggaactgg      7080
gccagaagct ggacttccct atggtgctaa caaagacggc atcatatggg ttgcaactga      7140
gggagccttg aatacaccaa aagatcacat tggcacccgc aatcctgcta acaatgctgc      7200
aatcgtgcta caacttcctc aaggaacaac attgccaaaa ggcttctacg cagaagggag      7260
aattgggagc ttcgcagcgt gtagcaggtg actcaggttt tgctgcatac agtcgctaca      7320
atcaaaacaa cgtcggcccc aaggtttacc caataatact gcgtcttggt tcaccgctct      7380
cactcaacat ggcaaggaag accttaaatt ccctcgagga caaggcgttc caattaacac      7440
caatagcagt ccagatgacc aaattggcta ctaccgaaga gctaccagac gaattcgtgg      7500
cagaggcggc agtcaagcct cttctcgttc ctcatcacgt agtcgcaaca gttcaagaaa      7560
ttcaactcca ggcagcagta ggggaacttc tcctgctaga atggctggca atggcggtga      7620
tgctgctctt gctttgctgc tgcttgacag attgaaccag cttgagagca aaatgtctgg      7680
taaaggccaa caacaacaag gccaaactgt cactaagaaa tctgctgctg aggcttctaa      7740
gaagcctcgg caaaaacgta ctgccactaa agcatacaat gtaacacaag ctttcggcag      7800
acgtggtcca gaacaaaccc aaggaaattt tggggaccag gaactaatca gacaaggaac      7860
tgattacaaa cattggccgc aaattgcaca atttgccccc agcgcttcag cgttcttcgg      7920
aatgtcgcgc attggcatgg aagtcacacc ttcgggaacg tggttgacct acacaggtgc      7980
tcagactgtt tgcgcgtacg cgttccatgt ggtcattcaa tccagaaact aacattcttc      8040
tcaacgtgcc actccatggc actattctga ccagaccgct tctagaaagt gaactcgtaa      8100
catcaaattg gatgacaaag atccaaattt caaagatcaa gtcattttgc tgaataagca      8160
tattgacgca tacaaaacat tcccaccaac agagcctaaa aaggacaaaa agaagaaggc      8220
tgatgaaact caagccttac cgcagagaca gaagaaacag caaactgtga ctcttcttcc      8280
tgctgcagat ttggatgatt tctccaaaca attgcaacaa tccatgagca gtgctgactc      8340
aactcaggcc taaactcatg cagaccacac aaggcagatg ggctatataa acgttttcgc      8400
ttttccgttt acgatatata gtctactctt gtgcagaatg aattctcgta actacatagc      8460
acaagtagat gtagttaact ttaatctcac atagcaatct ttaatcagtg tgtaacatta      8520
gggaggactt gaaagagcca ccacattttc accgaggcca cgcggagtac gatcgagtgt      8580
acagtgaaca atgctaggga gagctgccta tatggaagag ccctaatgtg taaaattaat      8640
tttagtagtg ctatccccat gtgattttaa tagcttctta ggagaatgac aaaaaaaaaa      8700
aaaaaaaaaa aaaaaaaaaa aaa                                              8723
 
