                         SEQUENCE LISTING

<110>  Monsanto Technology LLC
 
<120>  NOVEL INSECT INHIBITORY PROTEINS

<130>  MONS:485WO

<150>  US 63/130,385
<151>  2020-12-23

<160>  52    

<170>  PatentIn version 3.5

<210>  1
<211>  3471
<212>  DNA
<213>  Bacillus thuringiensis


<220>
<221>  misc_feature
<222>  (1)..(3471)
<223>  Nucleic acid sequence encoding a TIC4064 pesticidal protein 
       obtained from Bacillus thuringiensis species EG9820.

<400>  1
atgaatcaaa ataaacacgg aattattggc gcttccaatt gtggttgtac gtcagataat       60

gttgcgaaat atcctttagc caacaatcca tattcatctg ctttaaattt aaattcttgt      120

caaaatagta gtattctcaa ctggattaac ataataggcg atgcagcaaa agaagcagta      180

tctattggga caacaatagt ctctcttatc acagcacctt ctcttactgg attaatttca      240

atagtatatg accttatagg taaagtacta ggaggtagta gtggacaatc catatcagat      300

ttgtctatat gtgacttatt atctattatt gatttacggg taagtcagag tgttttaaat      360

gatgggattg cagattttaa tggttctgta ctcttataca ggaactattt agaggctctg      420

gatagctgga ataagaatcc taattctgct tctgctgaag aactccgtac tcgttttaga      480

atcgctgact cagaatttga tagaatttta acacgagggt ctttaacgaa tggtggctcg      540

ttagctagac aaaatgccca aatattatta ttaccttctt ttgcgagtgc tgcatttttc      600

catttattac tactaaggga tgctactaga tatggcacta attgggggct atacaatgct      660

acacctttta taaattatca atcaaaacta gtagagctta ttgaactata tactgattat      720

tgcgtacatt ggtataatcg aggtttcaac gaactaaggc aacgaggcac tagtgctaca      780

gcttggttag aatttcatag atatcgtaga gagatgacat tgatggtatt agatatagta      840

gcatcatttt caagtcttga tattactaat tacccaatag aaacagattt tcagttgagt      900

agggtcattt atacagatcc aattggtttt gtacatcgta gtagtcttag gggagaaagt      960

tggtttagct ttgttaatag agctaatttc tcagatttag aaaatgcaat acctaatcct     1020

agaccgtctt ggtttttaaa taatatgatt atatctactg gttcacttac attgccggtt     1080

agcccaagta ctgatagagc gagggtatgg tatggaagtc gagatcgaat ttcccctgct     1140

aattcacaat ttattactga actaatctct ggacaacata cgactgctac acaaactatt     1200

ttagggcgaa atatatttag agtagattct caagcttgta atttaaatga taccacatat     1260

ggagtgaata gggcggtatt ttatcatgat gcgagtgaag gttctcaaag atccgtgtac     1320

gaggggtata ttcgaacaac tgggatagat aaccctagag ttcaaaatat taacacttat     1380

ttacctggag aaaattcaga tatcccaact ccagaagact atactcatat attaagcaca     1440

acaataaatt taacaggagg acttagacaa gtagcatcta atcgccgttc atctttagta     1500

atgtatggtt ggacacataa aagtctggct cgtaacaata ccattaatcc agatagaatt     1560

acacagatac cattgacgaa ggttgatacc cgaggcacag gtgtttctta tgtgaatgat     1620

ccaggattta taggaggagc tctacttcaa aggactgacc atggttcgct tggagtattg     1680

agggtccaat ttccacttca cttaagacaa caatatcgca ttagagtccg ttatgcttct     1740

acaacaaata ttcgattgag tgtgaatggc agtttcggta ctatttctca aaatctccct     1800

agtacaatga gattaggaga ggatttaaga tacggatctt ttgctataag agagtttagt     1860

acttctatta gacccactgc aagtccggac caaattcgat tgacaataga accatctttt     1920

attagacaag aggtctatgt agatagaatt gagttcattc cagttaatcc gacgcgagag     1980

gcgaaagagg atctagaagc aacaaagaaa gcggtggcga gcttgtttac acgcacaagg     2040

gacggattac aagtaaatgt gacagattat caagtcgatc aagcggcaaa tttagtgtca     2100

tgcttatcag atgaacaata tgcgcatgat aaaaagatgt tattggaagc ggtacgcgcg     2160

gcaaaacgcc tcagccgaga acgcaactta cttcaggatc cagattttaa tacaatcaat     2220

agtacagaag aaaatggatg gaaagcaagt aacggcgtta ctattagcga aggcggtcca     2280

ttctataaag gccgtgcact tcagctagca agtgcacgag aaaattaccc aacatacatc     2340

tatcaaaaag tagatgcatc ggagttaaag ccgtatacac gttatagact ggatgggttc     2400

gtgaagagta gtcaagattt agaaattgat ctcattcacc atcataaagt ccatcttgtg     2460

aaaaatgcac cagataattt agtatctgat acttacccag atgattcttg tagtggaatc     2520

aatcgatgtc aggaacaaca gatggtaaat gcgcaactgg aaacagaaca tcatcatccg     2580

atggattgct gtggagcagc tcaaacacat gagttttctt cctatattga tacaggggat     2640

ttaaattcga ctgtagacca gggaatctgg gcgatcttta aagttcgaac aacagatggt     2700

tatgcgacgt taggaaatct tgaattggta gagatcggac cgttatcggg tgaatctcta     2760

gaacgtgaac aaagggataa tgcaaaatgg agtgcagagc taggaagaaa gcgtgcagaa     2820

acagatcgcg tgtatcaaga tgccaaacaa tccatcaatc atttatttgt ggattatcaa     2880

gatcaacaat taaatccaga aatagggatg gcagatatta tggacgctca aaatcttgtc     2940

gcatcaattt cagatgtata tagcgatgca gtactgcaaa tccctggaat taactatgag     3000

atttacacag agctatccaa tcgcttacaa caagcatcgt atctgtatac gtctcgaaat     3060

gcggtgcaaa atggggactt taacagcggt ctagatagtt ggaatgcaac agcgggtgct     3120

acggtacaac aggatggtaa tacgcatttc ttagttcttt ctcattggga tgcacaagtt     3180

tctcaacaat ttagagtgca gccaaattgt aaatatgtat tacgtgtaac agcagagaaa     3240

gtaggcggcg gagacggata cgtgacaatc cgggatggtg ctcatcatac agaaacgctt     3300

acatttaatg catgtgatta tgatataaat ggcacgtacg tgactgataa tacgtatcta     3360

acaaaagaag tggtattcta ttcacataca gaacacatgt gggtagaggt aagtgaaaca     3420

gaaggtgttt tccatataga cagtgttgag ttcatggaaa cccaacagta g              3471


<210>  2
<211>  1156
<212>  PRT
<213>  Bacillus thuringiensis


<220>
<221>  MISC_FEATURE
<222>  (1)..(1156)
<223>  The amino acid sequence of the TIC4064 pesticidal protein.

<400>  2

Met Asn Gln Asn Lys His Gly Ile Ile Gly Ala Ser Asn Cys Gly Cys 
1               5                   10                  15      


Thr Ser Asp Asn Val Ala Lys Tyr Pro Leu Ala Asn Asn Pro Tyr Ser 
            20                  25                  30          


Ser Ala Leu Asn Leu Asn Ser Cys Gln Asn Ser Ser Ile Leu Asn Trp 
        35                  40                  45              


Ile Asn Ile Ile Gly Asp Ala Ala Lys Glu Ala Val Ser Ile Gly Thr 
    50                  55                  60                  


Thr Ile Val Ser Leu Ile Thr Ala Pro Ser Leu Thr Gly Leu Ile Ser 
65                  70                  75                  80  


Ile Val Tyr Asp Leu Ile Gly Lys Val Leu Gly Gly Ser Ser Gly Gln 
                85                  90                  95      


Ser Ile Ser Asp Leu Ser Ile Cys Asp Leu Leu Ser Ile Ile Asp Leu 
            100                 105                 110         


Arg Val Ser Gln Ser Val Leu Asn Asp Gly Ile Ala Asp Phe Asn Gly 
        115                 120                 125             


Ser Val Leu Leu Tyr Arg Asn Tyr Leu Glu Ala Leu Asp Ser Trp Asn 
    130                 135                 140                 


Lys Asn Pro Asn Ser Ala Ser Ala Glu Glu Leu Arg Thr Arg Phe Arg 
145                 150                 155                 160 


Ile Ala Asp Ser Glu Phe Asp Arg Ile Leu Thr Arg Gly Ser Leu Thr 
                165                 170                 175     


Asn Gly Gly Ser Leu Ala Arg Gln Asn Ala Gln Ile Leu Leu Leu Pro 
            180                 185                 190         


Ser Phe Ala Ser Ala Ala Phe Phe His Leu Leu Leu Leu Arg Asp Ala 
        195                 200                 205             


Thr Arg Tyr Gly Thr Asn Trp Gly Leu Tyr Asn Ala Thr Pro Phe Ile 
    210                 215                 220                 


Asn Tyr Gln Ser Lys Leu Val Glu Leu Ile Glu Leu Tyr Thr Asp Tyr 
225                 230                 235                 240 


Cys Val His Trp Tyr Asn Arg Gly Phe Asn Glu Leu Arg Gln Arg Gly 
                245                 250                 255     


Thr Ser Ala Thr Ala Trp Leu Glu Phe His Arg Tyr Arg Arg Glu Met 
            260                 265                 270         


Thr Leu Met Val Leu Asp Ile Val Ala Ser Phe Ser Ser Leu Asp Ile 
        275                 280                 285             


Thr Asn Tyr Pro Ile Glu Thr Asp Phe Gln Leu Ser Arg Val Ile Tyr 
    290                 295                 300                 


Thr Asp Pro Ile Gly Phe Val His Arg Ser Ser Leu Arg Gly Glu Ser 
305                 310                 315                 320 


Trp Phe Ser Phe Val Asn Arg Ala Asn Phe Ser Asp Leu Glu Asn Ala 
                325                 330                 335     


Ile Pro Asn Pro Arg Pro Ser Trp Phe Leu Asn Asn Met Ile Ile Ser 
            340                 345                 350         


Thr Gly Ser Leu Thr Leu Pro Val Ser Pro Ser Thr Asp Arg Ala Arg 
        355                 360                 365             


Val Trp Tyr Gly Ser Arg Asp Arg Ile Ser Pro Ala Asn Ser Gln Phe 
    370                 375                 380                 


Ile Thr Glu Leu Ile Ser Gly Gln His Thr Thr Ala Thr Gln Thr Ile 
385                 390                 395                 400 


Leu Gly Arg Asn Ile Phe Arg Val Asp Ser Gln Ala Cys Asn Leu Asn 
                405                 410                 415     


Asp Thr Thr Tyr Gly Val Asn Arg Ala Val Phe Tyr His Asp Ala Ser 
            420                 425                 430         


Glu Gly Ser Gln Arg Ser Val Tyr Glu Gly Tyr Ile Arg Thr Thr Gly 
        435                 440                 445             


Ile Asp Asn Pro Arg Val Gln Asn Ile Asn Thr Tyr Leu Pro Gly Glu 
    450                 455                 460                 


Asn Ser Asp Ile Pro Thr Pro Glu Asp Tyr Thr His Ile Leu Ser Thr 
465                 470                 475                 480 


Thr Ile Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Ser Asn Arg Arg 
                485                 490                 495     


Ser Ser Leu Val Met Tyr Gly Trp Thr His Lys Ser Leu Ala Arg Asn 
            500                 505                 510         


Asn Thr Ile Asn Pro Asp Arg Ile Thr Gln Ile Pro Leu Thr Lys Val 
        515                 520                 525             


Asp Thr Arg Gly Thr Gly Val Ser Tyr Val Asn Asp Pro Gly Phe Ile 
    530                 535                 540                 


Gly Gly Ala Leu Leu Gln Arg Thr Asp His Gly Ser Leu Gly Val Leu 
545                 550                 555                 560 


Arg Val Gln Phe Pro Leu His Leu Arg Gln Gln Tyr Arg Ile Arg Val 
                565                 570                 575     


Arg Tyr Ala Ser Thr Thr Asn Ile Arg Leu Ser Val Asn Gly Ser Phe 
            580                 585                 590         


Gly Thr Ile Ser Gln Asn Leu Pro Ser Thr Met Arg Leu Gly Glu Asp 
        595                 600                 605             


Leu Arg Tyr Gly Ser Phe Ala Ile Arg Glu Phe Ser Thr Ser Ile Arg 
    610                 615                 620                 


Pro Thr Ala Ser Pro Asp Gln Ile Arg Leu Thr Ile Glu Pro Ser Phe 
625                 630                 635                 640 


Ile Arg Gln Glu Val Tyr Val Asp Arg Ile Glu Phe Ile Pro Val Asn 
                645                 650                 655     


Pro Thr Arg Glu Ala Lys Glu Asp Leu Glu Ala Thr Lys Lys Ala Val 
            660                 665                 670         


Ala Ser Leu Phe Thr Arg Thr Arg Asp Gly Leu Gln Val Asn Val Thr 
        675                 680                 685             


Asp Tyr Gln Val Asp Gln Ala Ala Asn Leu Val Ser Cys Leu Ser Asp 
    690                 695                 700                 


Glu Gln Tyr Ala His Asp Lys Lys Met Leu Leu Glu Ala Val Arg Ala 
705                 710                 715                 720 


Ala Lys Arg Leu Ser Arg Glu Arg Asn Leu Leu Gln Asp Pro Asp Phe 
                725                 730                 735     


Asn Thr Ile Asn Ser Thr Glu Glu Asn Gly Trp Lys Ala Ser Asn Gly 
            740                 745                 750         


Val Thr Ile Ser Glu Gly Gly Pro Phe Tyr Lys Gly Arg Ala Leu Gln 
        755                 760                 765             


Leu Ala Ser Ala Arg Glu Asn Tyr Pro Thr Tyr Ile Tyr Gln Lys Val 
    770                 775                 780                 


Asp Ala Ser Glu Leu Lys Pro Tyr Thr Arg Tyr Arg Leu Asp Gly Phe 
785                 790                 795                 800 


Val Lys Ser Ser Gln Asp Leu Glu Ile Asp Leu Ile His His His Lys 
                805                 810                 815     


Val His Leu Val Lys Asn Ala Pro Asp Asn Leu Val Ser Asp Thr Tyr 
            820                 825                 830         


Pro Asp Asp Ser Cys Ser Gly Ile Asn Arg Cys Gln Glu Gln Gln Met 
        835                 840                 845             


Val Asn Ala Gln Leu Glu Thr Glu His His His Pro Met Asp Cys Cys 
    850                 855                 860                 


Gly Ala Ala Gln Thr His Glu Phe Ser Ser Tyr Ile Asp Thr Gly Asp 
865                 870                 875                 880 


Leu Asn Ser Thr Val Asp Gln Gly Ile Trp Ala Ile Phe Lys Val Arg 
                885                 890                 895     


Thr Thr Asp Gly Tyr Ala Thr Leu Gly Asn Leu Glu Leu Val Glu Ile 
            900                 905                 910         


Gly Pro Leu Ser Gly Glu Ser Leu Glu Arg Glu Gln Arg Asp Asn Ala 
        915                 920                 925             


Lys Trp Ser Ala Glu Leu Gly Arg Lys Arg Ala Glu Thr Asp Arg Val 
    930                 935                 940                 


Tyr Gln Asp Ala Lys Gln Ser Ile Asn His Leu Phe Val Asp Tyr Gln 
945                 950                 955                 960 


Asp Gln Gln Leu Asn Pro Glu Ile Gly Met Ala Asp Ile Met Asp Ala 
                965                 970                 975     


Gln Asn Leu Val Ala Ser Ile Ser Asp Val Tyr Ser Asp Ala Val Leu 
            980                 985                 990         


Gln Ile Pro Gly Ile Asn Tyr Glu  Ile Tyr Thr Glu Leu  Ser Asn Arg 
        995                 1000                 1005             


Leu Gln  Gln Ala Ser Tyr Leu  Tyr Thr Ser Arg Asn  Ala Val Gln 
    1010                 1015                 1020             


Asn Gly  Asp Phe Asn Ser Gly  Leu Asp Ser Trp Asn  Ala Thr Ala 
    1025                 1030                 1035             


Gly Ala  Thr Val Gln Gln Asp  Gly Asn Thr His Phe  Leu Val Leu 
    1040                 1045                 1050             


Ser His  Trp Asp Ala Gln Val  Ser Gln Gln Phe Arg  Val Gln Pro 
    1055                 1060                 1065             


Asn Cys  Lys Tyr Val Leu Arg  Val Thr Ala Glu Lys  Val Gly Gly 
    1070                 1075                 1080             


Gly Asp  Gly Tyr Val Thr Ile  Arg Asp Gly Ala His  His Thr Glu 
    1085                 1090                 1095             


Thr Leu  Thr Phe Asn Ala Cys  Asp Tyr Asp Ile Asn  Gly Thr Tyr 
    1100                 1105                 1110             


Val Thr  Asp Asn Thr Tyr Leu  Thr Lys Glu Val Val  Phe Tyr Ser 
    1115                 1120                 1125             


His Thr  Glu His Met Trp Val  Glu Val Ser Glu Thr  Glu Gly Val 
    1130                 1135                 1140             


Phe His  Ile Asp Ser Val Glu  Phe Met Glu Thr Gln  Gln 
    1145                 1150                 1155     


<210>  3
<211>  3474
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic coding sequence encoding a TIC4064_1 pesticidal protein
       designed for expression in a plant cell wherein an additional 
       alanine codon is inserted immediately following the initiating 
       methionine codon.

<400>  3
atggctaacc agaacaagca cggcatcatt ggcgcatcca actgcgggtg taccagtgac       60

aacgtggcga aatacccgct cgccaataac ccatactcat cagccctgaa cctcaacagt      120

tgtcagaaca gctccatcct gaattggatc aacatcatcg gcgacgcggc gaaggaggcg      180

gtgagcatcg ggacaaccat cgtttcgctt atcacagctc cctcgctgac gggcctcatc      240

tcaatcgttt atgacctcat tgggaaggtg ctcggcggct cgtccggcca atccattagc      300

gacctttcca tctgcgatct gctctccata attgacctgc gggtgtcgca gtcggtcctg      360

aacgacggga tagcggactt caacggcagc gtcctcctgt accgcaacta cctggaggca      420

ctggactcct ggaacaagaa tcccaacagc gccagcgcgg aggagctgcg gacgcgcttt      480

cgcatcgctg attctgagtt tgaccgaatc ctgacacgcg gctcccttac caacggcggt      540

agcctggctc gccagaacgc ccagattctg ttgctgccca gtttcgcgtc agccgcattc      600

tttcacctac ttctccttcg ggacgcaacg cgctacggga ccaattgggg attgtacaac      660

gcgacgccct tcatcaacta tcagtctaag ctcgtcgaac tgatcgaact ctacacggac      720

tactgcgtcc actggtacaa tcgtggcttt aacgagttgc gtcaacgcgg aacttctgct      780

acggcgtggc ttgagtttca cagataccgt cgtgagatga cgctaatggt cctcgacatc      840

gtggcgtcgt tcagctcgct tgacatcacc aactacccta tcgagaccga ctttcaactt      900

agccgtgtga tctacactga cccaatcggc ttcgtccatc gaagttcact ccgtggcgag      960

tcctggttct cgttcgtgaa ccgcgccaac ttcagtgacc ttgagaatgc gatccctaac     1020

ccacgcccta gctggttcct gaacaacatg attatctcta cagggagcct gaccttaccc     1080

gtcagtccca gcacagatcg cgcgagagtc tggtacggga gtcgggaccg gatctcgccc     1140

gccaactctc agttcattac tgagcttatc tccgggcagc acaccaccgc cacccagaca     1200

atactcggtc gcaacatctt ccgcgtggat tcacaggcgt gcaacctaaa cgacaccacg     1260

tacggcgtca accgcgccgt gttctaccat gacgcctcgg agggctcgca gcgtagcgtt     1320

tatgagggct acatcaggac gacgggtatc gacaacccga gagtgcagaa catcaacacc     1380

tatttgccag gcgagaacag cgacatcccg acgcccgaag actacaccca catcctcagc     1440

acaaccatca acctcacggg cgggctccgc caagttgcct cgaaccggcg gagcagcctc     1500

gttatgtacg gctggaccca caagtctctg gcgcgcaaca acaccatcaa tccagatcga     1560

atcacccaga taccactcac gaaggtggat acccgaggga ctggcgttag ttacgtcaac     1620

gatcctggat tcatcggcgg cgcgcttctc caacgcacgg accacgggtc gctgggcgtc     1680

cttcgggtac agtttccgct acacttgcgc cagcaatacc ggataagggt tcggtacgcc     1740

tcaacaacga acatcagact gtctgtcaac ggctccttcg gcaccatctc ccagaacctt     1800

ccctccacca tgcgcctcgg tgaggatctg cgctacggct ctttcgctat ccgcgagttt     1860

agcacttcaa tacgcccaac cgcgtcacct gaccagatac gcctgaccat tgaaccgtcc     1920

ttcatccggc aagaggtgta cgtggacaga atcgagttca taccagtcaa cccgacgcgg     1980

gaggcgaagg aagaccttga ggctaccaag aaggcggtgg cgagcctgtt tacccgcact     2040

cgtgacggcc tccaagtaaa tgtcacggac taccaagtgg accaggcggc caacctcgta     2100

agctgcctat cagacgagca gtacgcccac gacaagaaga tgcttctgga agccgtcaga     2160

gccgccaaac ggctatcgcg cgagcgcaac ctgctccaag atcccgactt caacactatc     2220

aacagcaccg aggagaatgg ctggaaagcc agcaacggcg tgacgattag cgagggcggc     2280

ccgttctaca agggccgcgc actacagctt gcctccgcaa gggagaacta ccctacctac     2340

atctaccaga aggtcgatgc gtcggaactt aaaccataca cacggtatcg tctcgacggg     2400

ttcgtcaaat ccagccaaga cctagagatc gacctcatcc accatcacaa agtacatctc     2460

gtcaagaatg cgcccgacaa tcttgtctct gacacttacc cggacgattc ctgttcgggt     2520

attaacagat gccaggagca gcagatggtg aacgcccaac tcgaaaccga gcaccatcac     2580

ccaatggact gctgcggtgc ggctcagacc cacgagttta gcagttacat cgacaccggc     2640

gacctgaact cgacggtgga ccagggcatc tgggcgatct tcaaggttcg taccaccgac     2700

ggctacgcga ctctcgggaa tctggaactg gtcgagatcg gcccgttgag cggcgagtcg     2760

ctggagcggg agcagaggga caacgcaaag tggtctgccg agctgggccg aaagcgagcg     2820

gagactgacc gcgtgtacca ggatgctaag caaagtatca accacctctt cgtggactat     2880

caagatcaac agctcaaccc tgagatcggg atggctgaca tcatggacgc gcagaacctg     2940

gtggcgtcga tctccgatgt gtactccgac gccgtgctcc aaatccctgg cattaactac     3000

gagatctaca cggagctgtc gaaccgcctc caacaagcga gctaccttta cacgagccgg     3060

aacgccgtac agaacggcga cttcaactcc gggctcgact catggaacgc cactgcgggc     3120

gctaccgtcc aacaagacgg caacacccac tttctcgtcc ttagccactg ggacgcccaa     3180

gtctcgcagc agtttcgcgt tcagccgaac tgcaagtatg tcctgcgcgt gaccgccgag     3240

aaggtgggcg gtggtgacgg ctacgtcacc ataagggatg gtgcccacca cacggagacc     3300

ttaacgttta acgcctgtga ttatgacatc aacggcacct atgtgacgga caatacctac     3360

ctcactaagg aagtcgtgtt ctattcccat accgagcaca tgtgggtcga ggtttcggag     3420

acggagggcg tattccacat cgacagtgtg gagttcatgg agacccagca gtga           3474


<210>  4
<211>  1157
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of TIC4064_1 wherein an additional alanine 
       amino acid is inserted immediately following the initiating 
       methionine.

<400>  4

Met Ala Asn Gln Asn Lys His Gly Ile Ile Gly Ala Ser Asn Cys Gly 
1               5                   10                  15      


Cys Thr Ser Asp Asn Val Ala Lys Tyr Pro Leu Ala Asn Asn Pro Tyr 
            20                  25                  30          


Ser Ser Ala Leu Asn Leu Asn Ser Cys Gln Asn Ser Ser Ile Leu Asn 
        35                  40                  45              


Trp Ile Asn Ile Ile Gly Asp Ala Ala Lys Glu Ala Val Ser Ile Gly 
    50                  55                  60                  


Thr Thr Ile Val Ser Leu Ile Thr Ala Pro Ser Leu Thr Gly Leu Ile 
65                  70                  75                  80  


Ser Ile Val Tyr Asp Leu Ile Gly Lys Val Leu Gly Gly Ser Ser Gly 
                85                  90                  95      


Gln Ser Ile Ser Asp Leu Ser Ile Cys Asp Leu Leu Ser Ile Ile Asp 
            100                 105                 110         


Leu Arg Val Ser Gln Ser Val Leu Asn Asp Gly Ile Ala Asp Phe Asn 
        115                 120                 125             


Gly Ser Val Leu Leu Tyr Arg Asn Tyr Leu Glu Ala Leu Asp Ser Trp 
    130                 135                 140                 


Asn Lys Asn Pro Asn Ser Ala Ser Ala Glu Glu Leu Arg Thr Arg Phe 
145                 150                 155                 160 


Arg Ile Ala Asp Ser Glu Phe Asp Arg Ile Leu Thr Arg Gly Ser Leu 
                165                 170                 175     


Thr Asn Gly Gly Ser Leu Ala Arg Gln Asn Ala Gln Ile Leu Leu Leu 
            180                 185                 190         


Pro Ser Phe Ala Ser Ala Ala Phe Phe His Leu Leu Leu Leu Arg Asp 
        195                 200                 205             


Ala Thr Arg Tyr Gly Thr Asn Trp Gly Leu Tyr Asn Ala Thr Pro Phe 
    210                 215                 220                 


Ile Asn Tyr Gln Ser Lys Leu Val Glu Leu Ile Glu Leu Tyr Thr Asp 
225                 230                 235                 240 


Tyr Cys Val His Trp Tyr Asn Arg Gly Phe Asn Glu Leu Arg Gln Arg 
                245                 250                 255     


Gly Thr Ser Ala Thr Ala Trp Leu Glu Phe His Arg Tyr Arg Arg Glu 
            260                 265                 270         


Met Thr Leu Met Val Leu Asp Ile Val Ala Ser Phe Ser Ser Leu Asp 
        275                 280                 285             


Ile Thr Asn Tyr Pro Ile Glu Thr Asp Phe Gln Leu Ser Arg Val Ile 
    290                 295                 300                 


Tyr Thr Asp Pro Ile Gly Phe Val His Arg Ser Ser Leu Arg Gly Glu 
305                 310                 315                 320 


Ser Trp Phe Ser Phe Val Asn Arg Ala Asn Phe Ser Asp Leu Glu Asn 
                325                 330                 335     


Ala Ile Pro Asn Pro Arg Pro Ser Trp Phe Leu Asn Asn Met Ile Ile 
            340                 345                 350         


Ser Thr Gly Ser Leu Thr Leu Pro Val Ser Pro Ser Thr Asp Arg Ala 
        355                 360                 365             


Arg Val Trp Tyr Gly Ser Arg Asp Arg Ile Ser Pro Ala Asn Ser Gln 
    370                 375                 380                 


Phe Ile Thr Glu Leu Ile Ser Gly Gln His Thr Thr Ala Thr Gln Thr 
385                 390                 395                 400 


Ile Leu Gly Arg Asn Ile Phe Arg Val Asp Ser Gln Ala Cys Asn Leu 
                405                 410                 415     


Asn Asp Thr Thr Tyr Gly Val Asn Arg Ala Val Phe Tyr His Asp Ala 
            420                 425                 430         


Ser Glu Gly Ser Gln Arg Ser Val Tyr Glu Gly Tyr Ile Arg Thr Thr 
        435                 440                 445             


Gly Ile Asp Asn Pro Arg Val Gln Asn Ile Asn Thr Tyr Leu Pro Gly 
    450                 455                 460                 


Glu Asn Ser Asp Ile Pro Thr Pro Glu Asp Tyr Thr His Ile Leu Ser 
465                 470                 475                 480 


Thr Thr Ile Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Ser Asn Arg 
                485                 490                 495     


Arg Ser Ser Leu Val Met Tyr Gly Trp Thr His Lys Ser Leu Ala Arg 
            500                 505                 510         


Asn Asn Thr Ile Asn Pro Asp Arg Ile Thr Gln Ile Pro Leu Thr Lys 
        515                 520                 525             


Val Asp Thr Arg Gly Thr Gly Val Ser Tyr Val Asn Asp Pro Gly Phe 
    530                 535                 540                 


Ile Gly Gly Ala Leu Leu Gln Arg Thr Asp His Gly Ser Leu Gly Val 
545                 550                 555                 560 


Leu Arg Val Gln Phe Pro Leu His Leu Arg Gln Gln Tyr Arg Ile Arg 
                565                 570                 575     


Val Arg Tyr Ala Ser Thr Thr Asn Ile Arg Leu Ser Val Asn Gly Ser 
            580                 585                 590         


Phe Gly Thr Ile Ser Gln Asn Leu Pro Ser Thr Met Arg Leu Gly Glu 
        595                 600                 605             


Asp Leu Arg Tyr Gly Ser Phe Ala Ile Arg Glu Phe Ser Thr Ser Ile 
    610                 615                 620                 


Arg Pro Thr Ala Ser Pro Asp Gln Ile Arg Leu Thr Ile Glu Pro Ser 
625                 630                 635                 640 


Phe Ile Arg Gln Glu Val Tyr Val Asp Arg Ile Glu Phe Ile Pro Val 
                645                 650                 655     


Asn Pro Thr Arg Glu Ala Lys Glu Asp Leu Glu Ala Thr Lys Lys Ala 
            660                 665                 670         


Val Ala Ser Leu Phe Thr Arg Thr Arg Asp Gly Leu Gln Val Asn Val 
        675                 680                 685             


Thr Asp Tyr Gln Val Asp Gln Ala Ala Asn Leu Val Ser Cys Leu Ser 
    690                 695                 700                 


Asp Glu Gln Tyr Ala His Asp Lys Lys Met Leu Leu Glu Ala Val Arg 
705                 710                 715                 720 


Ala Ala Lys Arg Leu Ser Arg Glu Arg Asn Leu Leu Gln Asp Pro Asp 
                725                 730                 735     


Phe Asn Thr Ile Asn Ser Thr Glu Glu Asn Gly Trp Lys Ala Ser Asn 
            740                 745                 750         


Gly Val Thr Ile Ser Glu Gly Gly Pro Phe Tyr Lys Gly Arg Ala Leu 
        755                 760                 765             


Gln Leu Ala Ser Ala Arg Glu Asn Tyr Pro Thr Tyr Ile Tyr Gln Lys 
    770                 775                 780                 


Val Asp Ala Ser Glu Leu Lys Pro Tyr Thr Arg Tyr Arg Leu Asp Gly 
785                 790                 795                 800 


Phe Val Lys Ser Ser Gln Asp Leu Glu Ile Asp Leu Ile His His His 
                805                 810                 815     


Lys Val His Leu Val Lys Asn Ala Pro Asp Asn Leu Val Ser Asp Thr 
            820                 825                 830         


Tyr Pro Asp Asp Ser Cys Ser Gly Ile Asn Arg Cys Gln Glu Gln Gln 
        835                 840                 845             


Met Val Asn Ala Gln Leu Glu Thr Glu His His His Pro Met Asp Cys 
    850                 855                 860                 


Cys Gly Ala Ala Gln Thr His Glu Phe Ser Ser Tyr Ile Asp Thr Gly 
865                 870                 875                 880 


Asp Leu Asn Ser Thr Val Asp Gln Gly Ile Trp Ala Ile Phe Lys Val 
                885                 890                 895     


Arg Thr Thr Asp Gly Tyr Ala Thr Leu Gly Asn Leu Glu Leu Val Glu 
            900                 905                 910         


Ile Gly Pro Leu Ser Gly Glu Ser Leu Glu Arg Glu Gln Arg Asp Asn 
        915                 920                 925             


Ala Lys Trp Ser Ala Glu Leu Gly Arg Lys Arg Ala Glu Thr Asp Arg 
    930                 935                 940                 


Val Tyr Gln Asp Ala Lys Gln Ser Ile Asn His Leu Phe Val Asp Tyr 
945                 950                 955                 960 


Gln Asp Gln Gln Leu Asn Pro Glu Ile Gly Met Ala Asp Ile Met Asp 
                965                 970                 975     


Ala Gln Asn Leu Val Ala Ser Ile Ser Asp Val Tyr Ser Asp Ala Val 
            980                 985                 990         


Leu Gln Ile Pro Gly Ile Asn Tyr  Glu Ile Tyr Thr Glu  Leu Ser Asn 
        995                 1000                 1005             


Arg Leu  Gln Gln Ala Ser Tyr  Leu Tyr Thr Ser Arg  Asn Ala Val 
    1010                 1015                 1020             


Gln Asn  Gly Asp Phe Asn Ser  Gly Leu Asp Ser Trp  Asn Ala Thr 
    1025                 1030                 1035             


Ala Gly  Ala Thr Val Gln Gln  Asp Gly Asn Thr His  Phe Leu Val 
    1040                 1045                 1050             


Leu Ser  His Trp Asp Ala Gln  Val Ser Gln Gln Phe  Arg Val Gln 
    1055                 1060                 1065             


Pro Asn  Cys Lys Tyr Val Leu  Arg Val Thr Ala Glu  Lys Val Gly 
    1070                 1075                 1080             


Gly Gly  Asp Gly Tyr Val Thr  Ile Arg Asp Gly Ala  His His Thr 
    1085                 1090                 1095             


Glu Thr  Leu Thr Phe Asn Ala  Cys Asp Tyr Asp Ile  Asn Gly Thr 
    1100                 1105                 1110             


Tyr Val  Thr Asp Asn Thr Tyr  Leu Thr Lys Glu Val  Val Phe Tyr 
    1115                 1120                 1125             


Ser His  Thr Glu His Met Trp  Val Glu Val Ser Glu  Thr Glu Gly 
    1130                 1135                 1140             


Val Phe  His Ile Asp Ser Val  Glu Phe Met Glu Thr  Gln Gln 
    1145                 1150                 1155         


<210>  5
<211>  1974
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic coding sequence encoding a TIC4064_2 pesticidal protein
       designed for expression in a plant cell wherein an additional 
       alanine codon is inserted immediately following the initiating 
       methionine codon and protoxin truncation.

<400>  5
atggctaacc agaacaagca cggcatcatt ggcgcatcca actgcgggtg taccagtgac       60

aacgtggcga aatacccgct cgccaataac ccatactcat cagccctgaa cctcaacagt      120

tgtcagaaca gctccatcct gaattggatc aacatcatcg gcgacgcggc gaaggaggcg      180

gtgagcatcg ggacaaccat cgtttcgctt atcacagctc cctcgctgac gggcctcatc      240

tcaatcgttt atgacctcat tgggaaggtg ctcggcggct cgtccggcca atccattagc      300

gacctttcca tctgcgatct gctctccata attgacctgc gggtgtcgca gtcggtcctg      360

aacgacggga tagcggactt caacggcagc gtcctcctgt accgcaacta cctggaggca      420

ctggactcct ggaacaagaa tcccaacagc gccagcgcgg aggagctgcg gacgcgcttt      480

cgcatcgctg attctgagtt tgaccgaatc ctgacacgcg gctcccttac caacggcggt      540

agcctggctc gccagaacgc ccagattctg ttgctgccca gtttcgcgtc agccgcattc      600

tttcacctac ttctccttcg ggacgcaacg cgctacggga ccaattgggg attgtacaac      660

gcgacgccct tcatcaacta tcagtctaag ctcgtcgaac tgatcgaact ctacacggac      720

tactgcgtcc actggtacaa tcgtggcttt aacgagttgc gtcaacgcgg aacttctgct      780

acggcgtggc ttgagtttca cagataccgt cgtgagatga cgctaatggt cctcgacatc      840

gtggcgtcgt tcagctcgct tgacatcacc aactacccta tcgagaccga ctttcaactt      900

agccgtgtga tctacactga cccaatcggc ttcgtccatc gaagttcact ccgtggcgag      960

tcctggttct cgttcgtgaa ccgcgccaac ttcagtgacc ttgagaatgc gatccctaac     1020

ccacgcccta gctggttcct gaacaacatg attatctcta cagggagcct gaccttaccc     1080

gtcagtccca gcacagatcg cgcgagagtc tggtacggga gtcgggaccg gatctcgccc     1140

gccaactctc agttcattac tgagcttatc tccgggcagc acaccaccgc cacccagaca     1200

atactcggtc gcaacatctt ccgcgtggat tcacaggcgt gcaacctaaa cgacaccacg     1260

tacggcgtca accgcgccgt gttctaccat gacgcctcgg agggctcgca gcgtagcgtt     1320

tatgagggct acatcaggac gacgggtatc gacaacccga gagtgcagaa catcaacacc     1380

tatttgccag gcgagaacag cgacatcccg acgcccgaag actacaccca catcctcagc     1440

acaaccatca acctcacggg cgggctccgc caagttgcct cgaaccggcg gagcagcctc     1500

gttatgtacg gctggaccca caagtctctg gcgcgcaaca acaccatcaa tccagatcga     1560

atcacccaga taccactcac gaaggtggat acccgaggga ctggcgttag ttacgtcaac     1620

gatcctggat tcatcggcgg cgcgcttctc caacgcacgg accacgggtc gctgggcgtc     1680

cttcgggtac agtttccgct acacttgcgc cagcaatacc ggataagggt tcggtacgcc     1740

tcaacaacga acatcagact gtctgtcaac ggctccttcg gcaccatctc ccagaacctt     1800

ccctccacca tgcgcctcgg tgaggatctg cgctacggct ctttcgctat ccgcgagttt     1860

agcacttcaa tacgcccaac cgcgtcacct gaccagatac gcctgaccat tgaaccgtcc     1920

ttcatccggc aagaggtgta cgtggacaga atcgagttca taccagtcaa ctga           1974


<210>  6
<211>  657
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of TIC4064_2  wherein an additional alanine 
       amino acid is inserted immediately following the initiating 
       methionine, and a truncation of the protoxin domain.

<400>  6

Met Ala Asn Gln Asn Lys His Gly Ile Ile Gly Ala Ser Asn Cys Gly 
1               5                   10                  15      


Cys Thr Ser Asp Asn Val Ala Lys Tyr Pro Leu Ala Asn Asn Pro Tyr 
            20                  25                  30          


Ser Ser Ala Leu Asn Leu Asn Ser Cys Gln Asn Ser Ser Ile Leu Asn 
        35                  40                  45              


Trp Ile Asn Ile Ile Gly Asp Ala Ala Lys Glu Ala Val Ser Ile Gly 
    50                  55                  60                  


Thr Thr Ile Val Ser Leu Ile Thr Ala Pro Ser Leu Thr Gly Leu Ile 
65                  70                  75                  80  


Ser Ile Val Tyr Asp Leu Ile Gly Lys Val Leu Gly Gly Ser Ser Gly 
                85                  90                  95      


Gln Ser Ile Ser Asp Leu Ser Ile Cys Asp Leu Leu Ser Ile Ile Asp 
            100                 105                 110         


Leu Arg Val Ser Gln Ser Val Leu Asn Asp Gly Ile Ala Asp Phe Asn 
        115                 120                 125             


Gly Ser Val Leu Leu Tyr Arg Asn Tyr Leu Glu Ala Leu Asp Ser Trp 
    130                 135                 140                 


Asn Lys Asn Pro Asn Ser Ala Ser Ala Glu Glu Leu Arg Thr Arg Phe 
145                 150                 155                 160 


Arg Ile Ala Asp Ser Glu Phe Asp Arg Ile Leu Thr Arg Gly Ser Leu 
                165                 170                 175     


Thr Asn Gly Gly Ser Leu Ala Arg Gln Asn Ala Gln Ile Leu Leu Leu 
            180                 185                 190         


Pro Ser Phe Ala Ser Ala Ala Phe Phe His Leu Leu Leu Leu Arg Asp 
        195                 200                 205             


Ala Thr Arg Tyr Gly Thr Asn Trp Gly Leu Tyr Asn Ala Thr Pro Phe 
    210                 215                 220                 


Ile Asn Tyr Gln Ser Lys Leu Val Glu Leu Ile Glu Leu Tyr Thr Asp 
225                 230                 235                 240 


Tyr Cys Val His Trp Tyr Asn Arg Gly Phe Asn Glu Leu Arg Gln Arg 
                245                 250                 255     


Gly Thr Ser Ala Thr Ala Trp Leu Glu Phe His Arg Tyr Arg Arg Glu 
            260                 265                 270         


Met Thr Leu Met Val Leu Asp Ile Val Ala Ser Phe Ser Ser Leu Asp 
        275                 280                 285             


Ile Thr Asn Tyr Pro Ile Glu Thr Asp Phe Gln Leu Ser Arg Val Ile 
    290                 295                 300                 


Tyr Thr Asp Pro Ile Gly Phe Val His Arg Ser Ser Leu Arg Gly Glu 
305                 310                 315                 320 


Ser Trp Phe Ser Phe Val Asn Arg Ala Asn Phe Ser Asp Leu Glu Asn 
                325                 330                 335     


Ala Ile Pro Asn Pro Arg Pro Ser Trp Phe Leu Asn Asn Met Ile Ile 
            340                 345                 350         


Ser Thr Gly Ser Leu Thr Leu Pro Val Ser Pro Ser Thr Asp Arg Ala 
        355                 360                 365             


Arg Val Trp Tyr Gly Ser Arg Asp Arg Ile Ser Pro Ala Asn Ser Gln 
    370                 375                 380                 


Phe Ile Thr Glu Leu Ile Ser Gly Gln His Thr Thr Ala Thr Gln Thr 
385                 390                 395                 400 


Ile Leu Gly Arg Asn Ile Phe Arg Val Asp Ser Gln Ala Cys Asn Leu 
                405                 410                 415     


Asn Asp Thr Thr Tyr Gly Val Asn Arg Ala Val Phe Tyr His Asp Ala 
            420                 425                 430         


Ser Glu Gly Ser Gln Arg Ser Val Tyr Glu Gly Tyr Ile Arg Thr Thr 
        435                 440                 445             


Gly Ile Asp Asn Pro Arg Val Gln Asn Ile Asn Thr Tyr Leu Pro Gly 
    450                 455                 460                 


Glu Asn Ser Asp Ile Pro Thr Pro Glu Asp Tyr Thr His Ile Leu Ser 
465                 470                 475                 480 


Thr Thr Ile Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Ser Asn Arg 
                485                 490                 495     


Arg Ser Ser Leu Val Met Tyr Gly Trp Thr His Lys Ser Leu Ala Arg 
            500                 505                 510         


Asn Asn Thr Ile Asn Pro Asp Arg Ile Thr Gln Ile Pro Leu Thr Lys 
        515                 520                 525             


Val Asp Thr Arg Gly Thr Gly Val Ser Tyr Val Asn Asp Pro Gly Phe 
    530                 535                 540                 


Ile Gly Gly Ala Leu Leu Gln Arg Thr Asp His Gly Ser Leu Gly Val 
545                 550                 555                 560 


Leu Arg Val Gln Phe Pro Leu His Leu Arg Gln Gln Tyr Arg Ile Arg 
                565                 570                 575     


Val Arg Tyr Ala Ser Thr Thr Asn Ile Arg Leu Ser Val Asn Gly Ser 
            580                 585                 590         


Phe Gly Thr Ile Ser Gln Asn Leu Pro Ser Thr Met Arg Leu Gly Glu 
        595                 600                 605             


Asp Leu Arg Tyr Gly Ser Phe Ala Ile Arg Glu Phe Ser Thr Ser Ile 
    610                 615                 620                 


Arg Pro Thr Ala Ser Pro Asp Gln Ile Arg Leu Thr Ile Glu Pro Ser 
625                 630                 635                 640 


Phe Ile Arg Gln Glu Val Tyr Val Asp Arg Ile Glu Phe Ile Pro Val 
                645                 650                 655     


Asn 
    


<210>  7
<211>  3474
<212>  DNA
<213>  Artificial

<220>
<223>  Plant synthetic coding sequence encoding a TIC4064_3 pesticidal 
       protein designed for expression in a plant cell wherein an 
       additional alanine codon is inserted immediately following the 
       initiating methionine codon and comprising a mutations at S95T.

<400>  7
atggctaacc agaacaagca cggcatcatt ggcgcatcca actgcgggtg taccagtgac       60

aacgtggcga aatacccgct cgccaataac ccatactcat cagccctgaa cctcaacagt      120

tgtcagaaca gctccatcct gaattggatc aacatcatcg gcgacgcggc gaaggaggcg      180

gtgagcatcg ggacaaccat cgtttcgctt atcacagctc cctcgctgac gggcctcatc      240

tcaatcgttt atgacctcat tgggaaggtg ctcggcggct cgaccggcca atccattagc      300

gacctttcca tctgcgatct gctctccata attgacctgc gggtgtcgca gtcggtcctg      360

aacgacggga tagcggactt caacggcagc gtcctcctgt accgcaacta cctggaggca      420

ctggactcct ggaacaagaa tcccaacagc gccagcgcgg aggagctgcg gacgcgcttt      480

cgcatcgctg attctgagtt tgaccgaatc ctgacacgcg gctcccttac caacggcggt      540

agcctggctc gccagaacgc ccagattctg ttgctgccca gtttcgcgtc agccgcattc      600

tttcacctac ttctccttcg ggacgcaacg cgctacggga ccaattgggg attgtacaac      660

gcgacgccct tcatcaacta tcagtctaag ctcgtcgaac tgatcgaact ctacacggac      720

tactgcgtcc actggtacaa tcgtggcttt aacgagttgc gtcaacgcgg aacttctgct      780

acggcgtggc ttgagtttca cagataccgt cgtgagatga cgctaatggt cctcgacatc      840

gtggcgtcgt tcagctcgct tgacatcacc aactacccta tcgagaccga ctttcaactt      900

agccgtgtga tctacactga cccaatcggc ttcgtccatc gaagttcact ccgtggcgag      960

tcctggttct cgttcgtgaa ccgcgccaac ttcagtgacc ttgagaatgc gatccctaac     1020

ccacgcccta gctggttcct gaacaacatg attatctcta cagggagcct gaccttaccc     1080

gtcagtccca gcacagatcg cgcgagagtc tggtacggga gtcgggaccg gatctcgccc     1140

gccaactctc agttcattac tgagcttatc tccgggcagc acaccaccgc cacccagaca     1200

atactcggtc gcaacatctt ccgcgtggat tcacaggcgt gcaacctaaa cgacaccacg     1260

tacggcgtca accgcgccgt gttctaccat gacgcctcgg agggctcgca gcgtagcgtt     1320

tatgagggct acatcaggac gacgggtatc gacaacccga gagtgcagaa catcaacacc     1380

tatttgccag gcgagaacag cgacatcccg acgcccgaag actacaccca catcctcagc     1440

acaaccatca acctcacggg cgggctccgc caagttgcct cgaaccggcg gagcagcctc     1500

gttatgtacg gctggaccca caagtctctg gcgcgcaaca acaccatcaa tccagatcga     1560

atcacccaga taccactcac gaaggtggat acccgaggga ctggcgttag ttacgtcaac     1620

gatcctggat tcatcggcgg cgcgcttctc caacgcacgg accacgggtc gctgggcgtc     1680

cttcgggtac agtttccgct acacttgcgc cagcaatacc ggataagggt tcggtacgcc     1740

tcaacaacga acatcagact gtctgtcaac ggctccttcg gcaccatctc ccagaacctt     1800

ccctccacca tgcgcctcgg tgaggatctg cgctacggct ctttcgctat ccgcgagttt     1860

agcacttcaa tacgcccaac cgcgtcacct gaccagatac gcctgaccat tgaaccgtcc     1920

ttcatccggc aagaggtgta cgtggacaga atcgagttca taccagtcaa cccgacgcgg     1980

gaggcgaagg aagaccttga ggctaccaag aaggcggtgg cgagcctgtt tacccgcact     2040

cgtgacggcc tccaagtaaa tgtcacggac taccaagtgg accaggcggc caacctcgta     2100

agctgcctat cagacgagca gtacgcccac gacaagaaga tgcttctgga agccgtcaga     2160

gccgccaaac ggctatcgcg cgagcgcaac ctgctccaag atcccgactt caacactatc     2220

aacagcaccg aggagaatgg ctggaaagcc agcaacggcg tgacgattag cgagggcggc     2280

ccgttctaca agggccgcgc actacagctt gcctccgcaa gggagaacta ccctacctac     2340

atctaccaga aggtcgatgc gtcggaactt aaaccataca cacggtatcg tctcgacggg     2400

ttcgtcaaat ccagccaaga cctagagatc gacctcatcc accatcacaa agtacatctc     2460

gtcaagaatg cgcccgacaa tcttgtctct gacacttacc cggacgattc ctgttcgggt     2520

attaacagat gccaggagca gcagatggtg aacgcccaac tcgaaaccga gcaccatcac     2580

ccaatggact gctgcggtgc ggctcagacc cacgagttta gcagttacat cgacaccggc     2640

gacctgaact cgacggtgga ccagggcatc tgggcgatct tcaaggttcg taccaccgac     2700

ggctacgcga ctctcgggaa tctggaactg gtcgagatcg gcccgttgag cggcgagtcg     2760

ctggagcggg agcagaggga caacgcaaag tggtctgccg agctgggccg aaagcgagcg     2820

gagactgacc gcgtgtacca ggatgctaag caaagtatca accacctctt cgtggactat     2880

caagatcaac agctcaaccc tgagatcggg atggctgaca tcatggacgc gcagaacctg     2940

gtggcgtcga tctccgatgt gtactccgac gccgtgctcc aaatccctgg cattaactac     3000

gagatctaca cggagctgtc gaaccgcctc caacaagcga gctaccttta cacgagccgg     3060

aacgccgtac agaacggcga cttcaactcc gggctcgact catggaacgc cactgcgggc     3120

gctaccgtcc aacaagacgg caacacccac tttctcgtcc ttagccactg ggacgcccaa     3180

gtctcgcagc agtttcgcgt tcagccgaac tgcaagtatg tcctgcgcgt gaccgccgag     3240

aaggtgggcg gtggtgacgg ctacgtcacc ataagggatg gtgcccacca cacggagacc     3300

ttaacgttta acgcctgtga ttatgacatc aacggcacct atgtgacgga caatacctac     3360

ctcactaagg aagtcgtgtt ctattcccat accgagcaca tgtgggtcga ggtttcggag     3420

acggagggcg tattccacat cgacagtgtg gagttcatgg agacccagca gtga           3474


<210>  8
<211>  1157
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of TIC4064_3  wherein an additional alanine 
       amino acid is inserted immediately following the initiating 
       methionine and comprising a mutation at S95T.

<400>  8

Met Ala Asn Gln Asn Lys His Gly Ile Ile Gly Ala Ser Asn Cys Gly 
1               5                   10                  15      


Cys Thr Ser Asp Asn Val Ala Lys Tyr Pro Leu Ala Asn Asn Pro Tyr 
            20                  25                  30          


Ser Ser Ala Leu Asn Leu Asn Ser Cys Gln Asn Ser Ser Ile Leu Asn 
        35                  40                  45              


Trp Ile Asn Ile Ile Gly Asp Ala Ala Lys Glu Ala Val Ser Ile Gly 
    50                  55                  60                  


Thr Thr Ile Val Ser Leu Ile Thr Ala Pro Ser Leu Thr Gly Leu Ile 
65                  70                  75                  80  


Ser Ile Val Tyr Asp Leu Ile Gly Lys Val Leu Gly Gly Ser Thr Gly 
                85                  90                  95      


Gln Ser Ile Ser Asp Leu Ser Ile Cys Asp Leu Leu Ser Ile Ile Asp 
            100                 105                 110         


Leu Arg Val Ser Gln Ser Val Leu Asn Asp Gly Ile Ala Asp Phe Asn 
        115                 120                 125             


Gly Ser Val Leu Leu Tyr Arg Asn Tyr Leu Glu Ala Leu Asp Ser Trp 
    130                 135                 140                 


Asn Lys Asn Pro Asn Ser Ala Ser Ala Glu Glu Leu Arg Thr Arg Phe 
145                 150                 155                 160 


Arg Ile Ala Asp Ser Glu Phe Asp Arg Ile Leu Thr Arg Gly Ser Leu 
                165                 170                 175     


Thr Asn Gly Gly Ser Leu Ala Arg Gln Asn Ala Gln Ile Leu Leu Leu 
            180                 185                 190         


Pro Ser Phe Ala Ser Ala Ala Phe Phe His Leu Leu Leu Leu Arg Asp 
        195                 200                 205             


Ala Thr Arg Tyr Gly Thr Asn Trp Gly Leu Tyr Asn Ala Thr Pro Phe 
    210                 215                 220                 


Ile Asn Tyr Gln Ser Lys Leu Val Glu Leu Ile Glu Leu Tyr Thr Asp 
225                 230                 235                 240 


Tyr Cys Val His Trp Tyr Asn Arg Gly Phe Asn Glu Leu Arg Gln Arg 
                245                 250                 255     


Gly Thr Ser Ala Thr Ala Trp Leu Glu Phe His Arg Tyr Arg Arg Glu 
            260                 265                 270         


Met Thr Leu Met Val Leu Asp Ile Val Ala Ser Phe Ser Ser Leu Asp 
        275                 280                 285             


Ile Thr Asn Tyr Pro Ile Glu Thr Asp Phe Gln Leu Ser Arg Val Ile 
    290                 295                 300                 


Tyr Thr Asp Pro Ile Gly Phe Val His Arg Ser Ser Leu Arg Gly Glu 
305                 310                 315                 320 


Ser Trp Phe Ser Phe Val Asn Arg Ala Asn Phe Ser Asp Leu Glu Asn 
                325                 330                 335     


Ala Ile Pro Asn Pro Arg Pro Ser Trp Phe Leu Asn Asn Met Ile Ile 
            340                 345                 350         


Ser Thr Gly Ser Leu Thr Leu Pro Val Ser Pro Ser Thr Asp Arg Ala 
        355                 360                 365             


Arg Val Trp Tyr Gly Ser Arg Asp Arg Ile Ser Pro Ala Asn Ser Gln 
    370                 375                 380                 


Phe Ile Thr Glu Leu Ile Ser Gly Gln His Thr Thr Ala Thr Gln Thr 
385                 390                 395                 400 


Ile Leu Gly Arg Asn Ile Phe Arg Val Asp Ser Gln Ala Cys Asn Leu 
                405                 410                 415     


Asn Asp Thr Thr Tyr Gly Val Asn Arg Ala Val Phe Tyr His Asp Ala 
            420                 425                 430         


Ser Glu Gly Ser Gln Arg Ser Val Tyr Glu Gly Tyr Ile Arg Thr Thr 
        435                 440                 445             


Gly Ile Asp Asn Pro Arg Val Gln Asn Ile Asn Thr Tyr Leu Pro Gly 
    450                 455                 460                 


Glu Asn Ser Asp Ile Pro Thr Pro Glu Asp Tyr Thr His Ile Leu Ser 
465                 470                 475                 480 


Thr Thr Ile Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Ser Asn Arg 
                485                 490                 495     


Arg Ser Ser Leu Val Met Tyr Gly Trp Thr His Lys Ser Leu Ala Arg 
            500                 505                 510         


Asn Asn Thr Ile Asn Pro Asp Arg Ile Thr Gln Ile Pro Leu Thr Lys 
        515                 520                 525             


Val Asp Thr Arg Gly Thr Gly Val Ser Tyr Val Asn Asp Pro Gly Phe 
    530                 535                 540                 


Ile Gly Gly Ala Leu Leu Gln Arg Thr Asp His Gly Ser Leu Gly Val 
545                 550                 555                 560 


Leu Arg Val Gln Phe Pro Leu His Leu Arg Gln Gln Tyr Arg Ile Arg 
                565                 570                 575     


Val Arg Tyr Ala Ser Thr Thr Asn Ile Arg Leu Ser Val Asn Gly Ser 
            580                 585                 590         


Phe Gly Thr Ile Ser Gln Asn Leu Pro Ser Thr Met Arg Leu Gly Glu 
        595                 600                 605             


Asp Leu Arg Tyr Gly Ser Phe Ala Ile Arg Glu Phe Ser Thr Ser Ile 
    610                 615                 620                 


Arg Pro Thr Ala Ser Pro Asp Gln Ile Arg Leu Thr Ile Glu Pro Ser 
625                 630                 635                 640 


Phe Ile Arg Gln Glu Val Tyr Val Asp Arg Ile Glu Phe Ile Pro Val 
                645                 650                 655     


Asn Pro Thr Arg Glu Ala Lys Glu Asp Leu Glu Ala Thr Lys Lys Ala 
            660                 665                 670         


Val Ala Ser Leu Phe Thr Arg Thr Arg Asp Gly Leu Gln Val Asn Val 
        675                 680                 685             


Thr Asp Tyr Gln Val Asp Gln Ala Ala Asn Leu Val Ser Cys Leu Ser 
    690                 695                 700                 


Asp Glu Gln Tyr Ala His Asp Lys Lys Met Leu Leu Glu Ala Val Arg 
705                 710                 715                 720 


Ala Ala Lys Arg Leu Ser Arg Glu Arg Asn Leu Leu Gln Asp Pro Asp 
                725                 730                 735     


Phe Asn Thr Ile Asn Ser Thr Glu Glu Asn Gly Trp Lys Ala Ser Asn 
            740                 745                 750         


Gly Val Thr Ile Ser Glu Gly Gly Pro Phe Tyr Lys Gly Arg Ala Leu 
        755                 760                 765             


Gln Leu Ala Ser Ala Arg Glu Asn Tyr Pro Thr Tyr Ile Tyr Gln Lys 
    770                 775                 780                 


Val Asp Ala Ser Glu Leu Lys Pro Tyr Thr Arg Tyr Arg Leu Asp Gly 
785                 790                 795                 800 


Phe Val Lys Ser Ser Gln Asp Leu Glu Ile Asp Leu Ile His His His 
                805                 810                 815     


Lys Val His Leu Val Lys Asn Ala Pro Asp Asn Leu Val Ser Asp Thr 
            820                 825                 830         


Tyr Pro Asp Asp Ser Cys Ser Gly Ile Asn Arg Cys Gln Glu Gln Gln 
        835                 840                 845             


Met Val Asn Ala Gln Leu Glu Thr Glu His His His Pro Met Asp Cys 
    850                 855                 860                 


Cys Gly Ala Ala Gln Thr His Glu Phe Ser Ser Tyr Ile Asp Thr Gly 
865                 870                 875                 880 


Asp Leu Asn Ser Thr Val Asp Gln Gly Ile Trp Ala Ile Phe Lys Val 
                885                 890                 895     


Arg Thr Thr Asp Gly Tyr Ala Thr Leu Gly Asn Leu Glu Leu Val Glu 
            900                 905                 910         


Ile Gly Pro Leu Ser Gly Glu Ser Leu Glu Arg Glu Gln Arg Asp Asn 
        915                 920                 925             


Ala Lys Trp Ser Ala Glu Leu Gly Arg Lys Arg Ala Glu Thr Asp Arg 
    930                 935                 940                 


Val Tyr Gln Asp Ala Lys Gln Ser Ile Asn His Leu Phe Val Asp Tyr 
945                 950                 955                 960 


Gln Asp Gln Gln Leu Asn Pro Glu Ile Gly Met Ala Asp Ile Met Asp 
                965                 970                 975     


Ala Gln Asn Leu Val Ala Ser Ile Ser Asp Val Tyr Ser Asp Ala Val 
            980                 985                 990         


Leu Gln Ile Pro Gly Ile Asn Tyr  Glu Ile Tyr Thr Glu  Leu Ser Asn 
        995                 1000                 1005             


Arg Leu  Gln Gln Ala Ser Tyr  Leu Tyr Thr Ser Arg  Asn Ala Val 
    1010                 1015                 1020             


Gln Asn  Gly Asp Phe Asn Ser  Gly Leu Asp Ser Trp  Asn Ala Thr 
    1025                 1030                 1035             


Ala Gly  Ala Thr Val Gln Gln  Asp Gly Asn Thr His  Phe Leu Val 
    1040                 1045                 1050             


Leu Ser  His Trp Asp Ala Gln  Val Ser Gln Gln Phe  Arg Val Gln 
    1055                 1060                 1065             


Pro Asn  Cys Lys Tyr Val Leu  Arg Val Thr Ala Glu  Lys Val Gly 
    1070                 1075                 1080             


Gly Gly  Asp Gly Tyr Val Thr  Ile Arg Asp Gly Ala  His His Thr 
    1085                 1090                 1095             


Glu Thr  Leu Thr Phe Asn Ala  Cys Asp Tyr Asp Ile  Asn Gly Thr 
    1100                 1105                 1110             


Tyr Val  Thr Asp Asn Thr Tyr  Leu Thr Lys Glu Val  Val Phe Tyr 
    1115                 1120                 1125             


Ser His  Thr Glu His Met Trp  Val Glu Val Ser Glu  Thr Glu Gly 
    1130                 1135                 1140             


Val Phe  His Ile Asp Ser Val  Glu Phe Met Glu Thr  Gln Gln 
    1145                 1150                 1155         


<210>  9
<211>  1974
<212>  DNA
<213>  Artificial

<220>
<223>  Plant synthetic coding sequence encoding a TIC4064_4 pesticidal 
       protein designed for expression in a plant cell with a truncation
       of the protoxin domain and a mutation at S95T.

<400>  9
atggctaacc agaacaagca cggcatcatt ggcgcatcca actgcgggtg taccagtgac       60

aacgtggcga aatacccgct cgccaataac ccatactcat cagccctgaa cctcaacagt      120

tgtcagaaca gctccatcct gaattggatc aacatcatcg gcgacgcggc gaaggaggcg      180

gtgagcatcg ggacaaccat cgtttcgctt atcacagctc cctcgctgac gggcctcatc      240

tcaatcgttt atgacctcat tgggaaggtg ctcggcggct cgaccggcca atccattagc      300

gacctttcca tctgcgatct gctctccata attgacctgc gggtgtcgca gtcggtcctg      360

aacgacggga tagcggactt caacggcagc gtcctcctgt accgcaacta cctggaggca      420

ctggactcct ggaacaagaa tcccaacagc gccagcgcgg aggagctgcg gacgcgcttt      480

cgcatcgctg attctgagtt tgaccgaatc ctgacacgcg gctcccttac caacggcggt      540

agcctggctc gccagaacgc ccagattctg ttgctgccca gtttcgcgtc agccgcattc      600

tttcacctac ttctccttcg ggacgcaacg cgctacggga ccaattgggg attgtacaac      660

gcgacgccct tcatcaacta tcagtctaag ctcgtcgaac tgatcgaact ctacacggac      720

tactgcgtcc actggtacaa tcgtggcttt aacgagttgc gtcaacgcgg aacttctgct      780

acggcgtggc ttgagtttca cagataccgt cgtgagatga cgctaatggt cctcgacatc      840

gtggcgtcgt tcagctcgct tgacatcacc aactacccta tcgagaccga ctttcaactt      900

agccgtgtga tctacactga cccaatcggc ttcgtccatc gaagttcact ccgtggcgag      960

tcctggttct cgttcgtgaa ccgcgccaac ttcagtgacc ttgagaatgc gatccctaac     1020

ccacgcccta gctggttcct gaacaacatg attatctcta cagggagcct gaccttaccc     1080

gtcagtccca gcacagatcg cgcgagagtc tggtacggga gtcgggaccg gatctcgccc     1140

gccaactctc agttcattac tgagcttatc tccgggcagc acaccaccgc cacccagaca     1200

atactcggtc gcaacatctt ccgcgtggat tcacaggcgt gcaacctaaa cgacaccacg     1260

tacggcgtca accgcgccgt gttctaccat gacgcctcgg agggctcgca gcgtagcgtt     1320

tatgagggct acatcaggac gacgggtatc gacaacccga gagtgcagaa catcaacacc     1380

tatttgccag gcgagaacag cgacatcccg acgcccgaag actacaccca catcctcagc     1440

acaaccatca acctcacggg cgggctccgc caagttgcct cgaaccggcg gagcagcctc     1500

gttatgtacg gctggaccca caagtctctg gcgcgcaaca acaccatcaa tccagatcga     1560

atcacccaga taccactcac gaaggtggat acccgaggga ctggcgttag ttacgtcaac     1620

gatcctggat tcatcggcgg cgcgcttctc caacgcacgg accacgggtc gctgggcgtc     1680

cttcgggtac agtttccgct acacttgcgc cagcaatacc ggataagggt tcggtacgcc     1740

tcaacaacga acatcagact gtctgtcaac ggctccttcg gcaccatctc ccagaacctt     1800

ccctccacca tgcgcctcgg tgaggatctg cgctacggct ctttcgctat ccgcgagttt     1860

agcacttcaa tacgcccaac cgcgtcacct gaccagatac gcctgaccat tgaaccgtcc     1920

ttcatccggc aagaggtgta cgtggacaga atcgagttca taccagtcaa ctga           1974


<210>  10
<211>  657
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of TIC4064_4 wherein an additional alanine 
       amino acid is inserted immediately following the initiating 
       methionine and comprising a truncation of the protoxin domain and
       mutation at S95T.

<400>  10

Met Ala Asn Gln Asn Lys His Gly Ile Ile Gly Ala Ser Asn Cys Gly 
1               5                   10                  15      


Cys Thr Ser Asp Asn Val Ala Lys Tyr Pro Leu Ala Asn Asn Pro Tyr 
            20                  25                  30          


Ser Ser Ala Leu Asn Leu Asn Ser Cys Gln Asn Ser Ser Ile Leu Asn 
        35                  40                  45              


Trp Ile Asn Ile Ile Gly Asp Ala Ala Lys Glu Ala Val Ser Ile Gly 
    50                  55                  60                  


Thr Thr Ile Val Ser Leu Ile Thr Ala Pro Ser Leu Thr Gly Leu Ile 
65                  70                  75                  80  


Ser Ile Val Tyr Asp Leu Ile Gly Lys Val Leu Gly Gly Ser Thr Gly 
                85                  90                  95      


Gln Ser Ile Ser Asp Leu Ser Ile Cys Asp Leu Leu Ser Ile Ile Asp 
            100                 105                 110         


Leu Arg Val Ser Gln Ser Val Leu Asn Asp Gly Ile Ala Asp Phe Asn 
        115                 120                 125             


Gly Ser Val Leu Leu Tyr Arg Asn Tyr Leu Glu Ala Leu Asp Ser Trp 
    130                 135                 140                 


Asn Lys Asn Pro Asn Ser Ala Ser Ala Glu Glu Leu Arg Thr Arg Phe 
145                 150                 155                 160 


Arg Ile Ala Asp Ser Glu Phe Asp Arg Ile Leu Thr Arg Gly Ser Leu 
                165                 170                 175     


Thr Asn Gly Gly Ser Leu Ala Arg Gln Asn Ala Gln Ile Leu Leu Leu 
            180                 185                 190         


Pro Ser Phe Ala Ser Ala Ala Phe Phe His Leu Leu Leu Leu Arg Asp 
        195                 200                 205             


Ala Thr Arg Tyr Gly Thr Asn Trp Gly Leu Tyr Asn Ala Thr Pro Phe 
    210                 215                 220                 


Ile Asn Tyr Gln Ser Lys Leu Val Glu Leu Ile Glu Leu Tyr Thr Asp 
225                 230                 235                 240 


Tyr Cys Val His Trp Tyr Asn Arg Gly Phe Asn Glu Leu Arg Gln Arg 
                245                 250                 255     


Gly Thr Ser Ala Thr Ala Trp Leu Glu Phe His Arg Tyr Arg Arg Glu 
            260                 265                 270         


Met Thr Leu Met Val Leu Asp Ile Val Ala Ser Phe Ser Ser Leu Asp 
        275                 280                 285             


Ile Thr Asn Tyr Pro Ile Glu Thr Asp Phe Gln Leu Ser Arg Val Ile 
    290                 295                 300                 


Tyr Thr Asp Pro Ile Gly Phe Val His Arg Ser Ser Leu Arg Gly Glu 
305                 310                 315                 320 


Ser Trp Phe Ser Phe Val Asn Arg Ala Asn Phe Ser Asp Leu Glu Asn 
                325                 330                 335     


Ala Ile Pro Asn Pro Arg Pro Ser Trp Phe Leu Asn Asn Met Ile Ile 
            340                 345                 350         


Ser Thr Gly Ser Leu Thr Leu Pro Val Ser Pro Ser Thr Asp Arg Ala 
        355                 360                 365             


Arg Val Trp Tyr Gly Ser Arg Asp Arg Ile Ser Pro Ala Asn Ser Gln 
    370                 375                 380                 


Phe Ile Thr Glu Leu Ile Ser Gly Gln His Thr Thr Ala Thr Gln Thr 
385                 390                 395                 400 


Ile Leu Gly Arg Asn Ile Phe Arg Val Asp Ser Gln Ala Cys Asn Leu 
                405                 410                 415     


Asn Asp Thr Thr Tyr Gly Val Asn Arg Ala Val Phe Tyr His Asp Ala 
            420                 425                 430         


Ser Glu Gly Ser Gln Arg Ser Val Tyr Glu Gly Tyr Ile Arg Thr Thr 
        435                 440                 445             


Gly Ile Asp Asn Pro Arg Val Gln Asn Ile Asn Thr Tyr Leu Pro Gly 
    450                 455                 460                 


Glu Asn Ser Asp Ile Pro Thr Pro Glu Asp Tyr Thr His Ile Leu Ser 
465                 470                 475                 480 


Thr Thr Ile Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Ser Asn Arg 
                485                 490                 495     


Arg Ser Ser Leu Val Met Tyr Gly Trp Thr His Lys Ser Leu Ala Arg 
            500                 505                 510         


Asn Asn Thr Ile Asn Pro Asp Arg Ile Thr Gln Ile Pro Leu Thr Lys 
        515                 520                 525             


Val Asp Thr Arg Gly Thr Gly Val Ser Tyr Val Asn Asp Pro Gly Phe 
    530                 535                 540                 


Ile Gly Gly Ala Leu Leu Gln Arg Thr Asp His Gly Ser Leu Gly Val 
545                 550                 555                 560 


Leu Arg Val Gln Phe Pro Leu His Leu Arg Gln Gln Tyr Arg Ile Arg 
                565                 570                 575     


Val Arg Tyr Ala Ser Thr Thr Asn Ile Arg Leu Ser Val Asn Gly Ser 
            580                 585                 590         


Phe Gly Thr Ile Ser Gln Asn Leu Pro Ser Thr Met Arg Leu Gly Glu 
        595                 600                 605             


Asp Leu Arg Tyr Gly Ser Phe Ala Ile Arg Glu Phe Ser Thr Ser Ile 
    610                 615                 620                 


Arg Pro Thr Ala Ser Pro Asp Gln Ile Arg Leu Thr Ile Glu Pro Ser 
625                 630                 635                 640 


Phe Ile Arg Gln Glu Val Tyr Val Asp Arg Ile Glu Phe Ile Pro Val 
                645                 650                 655     


Asn 
    


<210>  11
<211>  3474
<212>  DNA
<213>  Artificial

<220>
<223>  Plant synthetic coding sequence encoding a TIC4064_5 pesticidal 
       protein designed for expression in a plant cell wherein an 
       additional alanine codon is inserted immediately following the 
       initiating methionine codon and a mutation at G88K.

<400>  11
atggctaacc agaacaagca cggcatcatt ggcgcatcca actgcgggtg taccagtgac       60

aacgtggcga aatacccgct cgccaataac ccatactcat cagccctgaa cctcaacagt      120

tgtcagaaca gctccatcct gaattggatc aacatcatcg gcgacgcggc gaaggaggcg      180

gtgagcatcg ggacaaccat cgtttcgctt atcacagctc cctcgctgac gggcctcatc      240

tcaatcgttt atgacctcat taagaaggtg ctcggcggct cgtccggcca atccattagc      300

gacctttcca tctgcgatct gctctccata attgacctgc gggtgtcgca gtcggtcctg      360

aacgacggga tagcggactt caacggcagc gtcctcctgt accgcaacta cctggaggca      420

ctggactcct ggaacaagaa tcccaacagc gccagcgcgg aggagctgcg gacgcgcttt      480

cgcatcgctg attctgagtt tgaccgaatc ctgacacgcg gctcccttac caacggcggt      540

agcctggctc gccagaacgc ccagattctg ttgctgccca gtttcgcgtc agccgcattc      600

tttcacctac ttctccttcg ggacgcaacg cgctacggga ccaattgggg attgtacaac      660

gcgacgccct tcatcaacta tcagtctaag ctcgtcgaac tgatcgaact ctacacggac      720

tactgcgtcc actggtacaa tcgtggcttt aacgagttgc gtcaacgcgg aacttctgct      780

acggcgtggc ttgagtttca cagataccgt cgtgagatga cgctaatggt cctcgacatc      840

gtggcgtcgt tcagctcgct tgacatcacc aactacccta tcgagaccga ctttcaactt      900

agccgtgtga tctacactga cccaatcggc ttcgtccatc gaagttcact ccgtggcgag      960

tcctggttct cgttcgtgaa ccgcgccaac ttcagtgacc ttgagaatgc gatccctaac     1020

ccacgcccta gctggttcct gaacaacatg attatctcta cagggagcct gaccttaccc     1080

gtcagtccca gcacagatcg cgcgagagtc tggtacggga gtcgggaccg gatctcgccc     1140

gccaactctc agttcattac tgagcttatc tccgggcagc acaccaccgc cacccagaca     1200

atactcggtc gcaacatctt ccgcgtggat tcacaggcgt gcaacctaaa cgacaccacg     1260

tacggcgtca accgcgccgt gttctaccat gacgcctcgg agggctcgca gcgtagcgtt     1320

tatgagggct acatcaggac gacgggtatc gacaacccga gagtgcagaa catcaacacc     1380

tatttgccag gcgagaacag cgacatcccg acgcccgaag actacaccca catcctcagc     1440

acaaccatca acctcacggg cgggctccgc caagttgcct cgaaccggcg gagcagcctc     1500

gttatgtacg gctggaccca caagtctctg gcgcgcaaca acaccatcaa tccagatcga     1560

atcacccaga taccactcac gaaggtggat acccgaggga ctggcgttag ttacgtcaac     1620

gatcctggat tcatcggcgg cgcgcttctc caacgcacgg accacgggtc gctgggcgtc     1680

cttcgggtac agtttccgct acacttgcgc cagcaatacc ggataagggt tcggtacgcc     1740

tcaacaacga acatcagact gtctgtcaac ggctccttcg gcaccatctc ccagaacctt     1800

ccctccacca tgcgcctcgg tgaggatctg cgctacggct ctttcgctat ccgcgagttt     1860

agcacttcaa tacgcccaac cgcgtcacct gaccagatac gcctgaccat tgaaccgtcc     1920

ttcatccggc aagaggtgta cgtggacaga atcgagttca taccagtcaa cccgacgcgg     1980

gaggcgaagg aagaccttga ggctaccaag aaggcggtgg cgagcctgtt tacccgcact     2040

cgtgacggcc tccaagtaaa tgtcacggac taccaagtgg accaggcggc caacctcgta     2100

agctgcctat cagacgagca gtacgcccac gacaagaaga tgcttctgga agccgtcaga     2160

gccgccaaac ggctatcgcg cgagcgcaac ctgctccaag atcccgactt caacactatc     2220

aacagcaccg aggagaatgg ctggaaagcc agcaacggcg tgacgattag cgagggcggc     2280

ccgttctaca agggccgcgc actacagctt gcctccgcaa gggagaacta ccctacctac     2340

atctaccaga aggtcgatgc gtcggaactt aaaccataca cacggtatcg tctcgacggg     2400

ttcgtcaaat ccagccaaga cctagagatc gacctcatcc accatcacaa agtacatctc     2460

gtcaagaatg cgcccgacaa tcttgtctct gacacttacc cggacgattc ctgttcgggt     2520

attaacagat gccaggagca gcagatggtg aacgcccaac tcgaaaccga gcaccatcac     2580

ccaatggact gctgcggtgc ggctcagacc cacgagttta gcagttacat cgacaccggc     2640

gacctgaact cgacggtgga ccagggcatc tgggcgatct tcaaggttcg taccaccgac     2700

ggctacgcga ctctcgggaa tctggaactg gtcgagatcg gcccgttgag cggcgagtcg     2760

ctggagcggg agcagaggga caacgcaaag tggtctgccg agctgggccg aaagcgagcg     2820

gagactgacc gcgtgtacca ggatgctaag caaagtatca accacctctt cgtggactat     2880

caagatcaac agctcaaccc tgagatcggg atggctgaca tcatggacgc gcagaacctg     2940

gtggcgtcga tctccgatgt gtactccgac gccgtgctcc aaatccctgg cattaactac     3000

gagatctaca cggagctgtc gaaccgcctc caacaagcga gctaccttta cacgagccgg     3060

aacgccgtac agaacggcga cttcaactcc gggctcgact catggaacgc cactgcgggc     3120

gctaccgtcc aacaagacgg caacacccac tttctcgtcc ttagccactg ggacgcccaa     3180

gtctcgcagc agtttcgcgt tcagccgaac tgcaagtatg tcctgcgcgt gaccgccgag     3240

aaggtgggcg gtggtgacgg ctacgtcacc ataagggatg gtgcccacca cacggagacc     3300

ttaacgttta acgcctgtga ttatgacatc aacggcacct atgtgacgga caatacctac     3360

ctcactaagg aagtcgtgtt ctattcccat accgagcaca tgtgggtcga ggtttcggag     3420

acggagggcg tattccacat cgacagtgtg gagttcatgg agacccagca gtga           3474


<210>  12
<211>  1157
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of TIC4064_5  wherein an additional alanine 
       amino acid is inserted immediately following the initiating 
       methionine and comprising a mutation at G88K.

<400>  12

Met Ala Asn Gln Asn Lys His Gly Ile Ile Gly Ala Ser Asn Cys Gly 
1               5                   10                  15      


Cys Thr Ser Asp Asn Val Ala Lys Tyr Pro Leu Ala Asn Asn Pro Tyr 
            20                  25                  30          


Ser Ser Ala Leu Asn Leu Asn Ser Cys Gln Asn Ser Ser Ile Leu Asn 
        35                  40                  45              


Trp Ile Asn Ile Ile Gly Asp Ala Ala Lys Glu Ala Val Ser Ile Gly 
    50                  55                  60                  


Thr Thr Ile Val Ser Leu Ile Thr Ala Pro Ser Leu Thr Gly Leu Ile 
65                  70                  75                  80  


Ser Ile Val Tyr Asp Leu Ile Lys Lys Val Leu Gly Gly Ser Ser Gly 
                85                  90                  95      


Gln Ser Ile Ser Asp Leu Ser Ile Cys Asp Leu Leu Ser Ile Ile Asp 
            100                 105                 110         


Leu Arg Val Ser Gln Ser Val Leu Asn Asp Gly Ile Ala Asp Phe Asn 
        115                 120                 125             


Gly Ser Val Leu Leu Tyr Arg Asn Tyr Leu Glu Ala Leu Asp Ser Trp 
    130                 135                 140                 


Asn Lys Asn Pro Asn Ser Ala Ser Ala Glu Glu Leu Arg Thr Arg Phe 
145                 150                 155                 160 


Arg Ile Ala Asp Ser Glu Phe Asp Arg Ile Leu Thr Arg Gly Ser Leu 
                165                 170                 175     


Thr Asn Gly Gly Ser Leu Ala Arg Gln Asn Ala Gln Ile Leu Leu Leu 
            180                 185                 190         


Pro Ser Phe Ala Ser Ala Ala Phe Phe His Leu Leu Leu Leu Arg Asp 
        195                 200                 205             


Ala Thr Arg Tyr Gly Thr Asn Trp Gly Leu Tyr Asn Ala Thr Pro Phe 
    210                 215                 220                 


Ile Asn Tyr Gln Ser Lys Leu Val Glu Leu Ile Glu Leu Tyr Thr Asp 
225                 230                 235                 240 


Tyr Cys Val His Trp Tyr Asn Arg Gly Phe Asn Glu Leu Arg Gln Arg 
                245                 250                 255     


Gly Thr Ser Ala Thr Ala Trp Leu Glu Phe His Arg Tyr Arg Arg Glu 
            260                 265                 270         


Met Thr Leu Met Val Leu Asp Ile Val Ala Ser Phe Ser Ser Leu Asp 
        275                 280                 285             


Ile Thr Asn Tyr Pro Ile Glu Thr Asp Phe Gln Leu Ser Arg Val Ile 
    290                 295                 300                 


Tyr Thr Asp Pro Ile Gly Phe Val His Arg Ser Ser Leu Arg Gly Glu 
305                 310                 315                 320 


Ser Trp Phe Ser Phe Val Asn Arg Ala Asn Phe Ser Asp Leu Glu Asn 
                325                 330                 335     


Ala Ile Pro Asn Pro Arg Pro Ser Trp Phe Leu Asn Asn Met Ile Ile 
            340                 345                 350         


Ser Thr Gly Ser Leu Thr Leu Pro Val Ser Pro Ser Thr Asp Arg Ala 
        355                 360                 365             


Arg Val Trp Tyr Gly Ser Arg Asp Arg Ile Ser Pro Ala Asn Ser Gln 
    370                 375                 380                 


Phe Ile Thr Glu Leu Ile Ser Gly Gln His Thr Thr Ala Thr Gln Thr 
385                 390                 395                 400 


Ile Leu Gly Arg Asn Ile Phe Arg Val Asp Ser Gln Ala Cys Asn Leu 
                405                 410                 415     


Asn Asp Thr Thr Tyr Gly Val Asn Arg Ala Val Phe Tyr His Asp Ala 
            420                 425                 430         


Ser Glu Gly Ser Gln Arg Ser Val Tyr Glu Gly Tyr Ile Arg Thr Thr 
        435                 440                 445             


Gly Ile Asp Asn Pro Arg Val Gln Asn Ile Asn Thr Tyr Leu Pro Gly 
    450                 455                 460                 


Glu Asn Ser Asp Ile Pro Thr Pro Glu Asp Tyr Thr His Ile Leu Ser 
465                 470                 475                 480 


Thr Thr Ile Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Ser Asn Arg 
                485                 490                 495     


Arg Ser Ser Leu Val Met Tyr Gly Trp Thr His Lys Ser Leu Ala Arg 
            500                 505                 510         


Asn Asn Thr Ile Asn Pro Asp Arg Ile Thr Gln Ile Pro Leu Thr Lys 
        515                 520                 525             


Val Asp Thr Arg Gly Thr Gly Val Ser Tyr Val Asn Asp Pro Gly Phe 
    530                 535                 540                 


Ile Gly Gly Ala Leu Leu Gln Arg Thr Asp His Gly Ser Leu Gly Val 
545                 550                 555                 560 


Leu Arg Val Gln Phe Pro Leu His Leu Arg Gln Gln Tyr Arg Ile Arg 
                565                 570                 575     


Val Arg Tyr Ala Ser Thr Thr Asn Ile Arg Leu Ser Val Asn Gly Ser 
            580                 585                 590         


Phe Gly Thr Ile Ser Gln Asn Leu Pro Ser Thr Met Arg Leu Gly Glu 
        595                 600                 605             


Asp Leu Arg Tyr Gly Ser Phe Ala Ile Arg Glu Phe Ser Thr Ser Ile 
    610                 615                 620                 


Arg Pro Thr Ala Ser Pro Asp Gln Ile Arg Leu Thr Ile Glu Pro Ser 
625                 630                 635                 640 


Phe Ile Arg Gln Glu Val Tyr Val Asp Arg Ile Glu Phe Ile Pro Val 
                645                 650                 655     


Asn Pro Thr Arg Glu Ala Lys Glu Asp Leu Glu Ala Thr Lys Lys Ala 
            660                 665                 670         


Val Ala Ser Leu Phe Thr Arg Thr Arg Asp Gly Leu Gln Val Asn Val 
        675                 680                 685             


Thr Asp Tyr Gln Val Asp Gln Ala Ala Asn Leu Val Ser Cys Leu Ser 
    690                 695                 700                 


Asp Glu Gln Tyr Ala His Asp Lys Lys Met Leu Leu Glu Ala Val Arg 
705                 710                 715                 720 


Ala Ala Lys Arg Leu Ser Arg Glu Arg Asn Leu Leu Gln Asp Pro Asp 
                725                 730                 735     


Phe Asn Thr Ile Asn Ser Thr Glu Glu Asn Gly Trp Lys Ala Ser Asn 
            740                 745                 750         


Gly Val Thr Ile Ser Glu Gly Gly Pro Phe Tyr Lys Gly Arg Ala Leu 
        755                 760                 765             


Gln Leu Ala Ser Ala Arg Glu Asn Tyr Pro Thr Tyr Ile Tyr Gln Lys 
    770                 775                 780                 


Val Asp Ala Ser Glu Leu Lys Pro Tyr Thr Arg Tyr Arg Leu Asp Gly 
785                 790                 795                 800 


Phe Val Lys Ser Ser Gln Asp Leu Glu Ile Asp Leu Ile His His His 
                805                 810                 815     


Lys Val His Leu Val Lys Asn Ala Pro Asp Asn Leu Val Ser Asp Thr 
            820                 825                 830         


Tyr Pro Asp Asp Ser Cys Ser Gly Ile Asn Arg Cys Gln Glu Gln Gln 
        835                 840                 845             


Met Val Asn Ala Gln Leu Glu Thr Glu His His His Pro Met Asp Cys 
    850                 855                 860                 


Cys Gly Ala Ala Gln Thr His Glu Phe Ser Ser Tyr Ile Asp Thr Gly 
865                 870                 875                 880 


Asp Leu Asn Ser Thr Val Asp Gln Gly Ile Trp Ala Ile Phe Lys Val 
                885                 890                 895     


Arg Thr Thr Asp Gly Tyr Ala Thr Leu Gly Asn Leu Glu Leu Val Glu 
            900                 905                 910         


Ile Gly Pro Leu Ser Gly Glu Ser Leu Glu Arg Glu Gln Arg Asp Asn 
        915                 920                 925             


Ala Lys Trp Ser Ala Glu Leu Gly Arg Lys Arg Ala Glu Thr Asp Arg 
    930                 935                 940                 


Val Tyr Gln Asp Ala Lys Gln Ser Ile Asn His Leu Phe Val Asp Tyr 
945                 950                 955                 960 


Gln Asp Gln Gln Leu Asn Pro Glu Ile Gly Met Ala Asp Ile Met Asp 
                965                 970                 975     


Ala Gln Asn Leu Val Ala Ser Ile Ser Asp Val Tyr Ser Asp Ala Val 
            980                 985                 990         


Leu Gln Ile Pro Gly Ile Asn Tyr  Glu Ile Tyr Thr Glu  Leu Ser Asn 
        995                 1000                 1005             


Arg Leu  Gln Gln Ala Ser Tyr  Leu Tyr Thr Ser Arg  Asn Ala Val 
    1010                 1015                 1020             


Gln Asn  Gly Asp Phe Asn Ser  Gly Leu Asp Ser Trp  Asn Ala Thr 
    1025                 1030                 1035             


Ala Gly  Ala Thr Val Gln Gln  Asp Gly Asn Thr His  Phe Leu Val 
    1040                 1045                 1050             


Leu Ser  His Trp Asp Ala Gln  Val Ser Gln Gln Phe  Arg Val Gln 
    1055                 1060                 1065             


Pro Asn  Cys Lys Tyr Val Leu  Arg Val Thr Ala Glu  Lys Val Gly 
    1070                 1075                 1080             


Gly Gly  Asp Gly Tyr Val Thr  Ile Arg Asp Gly Ala  His His Thr 
    1085                 1090                 1095             


Glu Thr  Leu Thr Phe Asn Ala  Cys Asp Tyr Asp Ile  Asn Gly Thr 
    1100                 1105                 1110             


Tyr Val  Thr Asp Asn Thr Tyr  Leu Thr Lys Glu Val  Val Phe Tyr 
    1115                 1120                 1125             


Ser His  Thr Glu His Met Trp  Val Glu Val Ser Glu  Thr Glu Gly 
    1130                 1135                 1140             


Val Phe  His Ile Asp Ser Val  Glu Phe Met Glu Thr  Gln Gln 
    1145                 1150                 1155         


<210>  13
<211>  1974
<212>  DNA
<213>  Artificial

<220>
<223>  Plant synthetic coding sequence encoding a TIC4064_6 pesticidal 
       protein with an additional alanine codon inserted immediately 
       following the initiating methionine and comprising a protoxin 
       truncation and G88K mutation.

<400>  13
atggctaacc agaacaagca cggcatcatt ggcgcatcca actgcgggtg taccagtgac       60

aacgtggcga aatacccgct cgccaataac ccatactcat cagccctgaa cctcaacagt      120

tgtcagaaca gctccatcct gaattggatc aacatcatcg gcgacgcggc gaaggaggcg      180

gtgagcatcg ggacaaccat cgtttcgctt atcacagctc cctcgctgac gggcctcatc      240

tcaatcgttt atgacctcat taagaaggtg ctcggcggct cgtccggcca atccattagc      300

gacctttcca tctgcgatct gctctccata attgacctgc gggtgtcgca gtcggtcctg      360

aacgacggga tagcggactt caacggcagc gtcctcctgt accgcaacta cctggaggca      420

ctggactcct ggaacaagaa tcccaacagc gccagcgcgg aggagctgcg gacgcgcttt      480

cgcatcgctg attctgagtt tgaccgaatc ctgacacgcg gctcccttac caacggcggt      540

agcctggctc gccagaacgc ccagattctg ttgctgccca gtttcgcgtc agccgcattc      600

tttcacctac ttctccttcg ggacgcaacg cgctacggga ccaattgggg attgtacaac      660

gcgacgccct tcatcaacta tcagtctaag ctcgtcgaac tgatcgaact ctacacggac      720

tactgcgtcc actggtacaa tcgtggcttt aacgagttgc gtcaacgcgg aacttctgct      780

acggcgtggc ttgagtttca cagataccgt cgtgagatga cgctaatggt cctcgacatc      840

gtggcgtcgt tcagctcgct tgacatcacc aactacccta tcgagaccga ctttcaactt      900

agccgtgtga tctacactga cccaatcggc ttcgtccatc gaagttcact ccgtggcgag      960

tcctggttct cgttcgtgaa ccgcgccaac ttcagtgacc ttgagaatgc gatccctaac     1020

ccacgcccta gctggttcct gaacaacatg attatctcta cagggagcct gaccttaccc     1080

gtcagtccca gcacagatcg cgcgagagtc tggtacggga gtcgggaccg gatctcgccc     1140

gccaactctc agttcattac tgagcttatc tccgggcagc acaccaccgc cacccagaca     1200

atactcggtc gcaacatctt ccgcgtggat tcacaggcgt gcaacctaaa cgacaccacg     1260

tacggcgtca accgcgccgt gttctaccat gacgcctcgg agggctcgca gcgtagcgtt     1320

tatgagggct acatcaggac gacgggtatc gacaacccga gagtgcagaa catcaacacc     1380

tatttgccag gcgagaacag cgacatcccg acgcccgaag actacaccca catcctcagc     1440

acaaccatca acctcacggg cgggctccgc caagttgcct cgaaccggcg gagcagcctc     1500

gttatgtacg gctggaccca caagtctctg gcgcgcaaca acaccatcaa tccagatcga     1560

atcacccaga taccactcac gaaggtggat acccgaggga ctggcgttag ttacgtcaac     1620

gatcctggat tcatcggcgg cgcgcttctc caacgcacgg accacgggtc gctgggcgtc     1680

cttcgggtac agtttccgct acacttgcgc cagcaatacc ggataagggt tcggtacgcc     1740

tcaacaacga acatcagact gtctgtcaac ggctccttcg gcaccatctc ccagaacctt     1800

ccctccacca tgcgcctcgg tgaggatctg cgctacggct ctttcgctat ccgcgagttt     1860

agcacttcaa tacgcccaac cgcgtcacct gaccagatac gcctgaccat tgaaccgtcc     1920

ttcatccggc aagaggtgta cgtggacaga atcgagttca taccagtcaa ctga           1974


<210>  14
<211>  657
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of TIC4064_4 wherein an additional alanine 
       amino acid is inserted immediately following the initiating 
       methionine, and comprising a protoxin domain truncation and a 
       G88K mutation.

<400>  14

Met Ala Asn Gln Asn Lys His Gly Ile Ile Gly Ala Ser Asn Cys Gly 
1               5                   10                  15      


Cys Thr Ser Asp Asn Val Ala Lys Tyr Pro Leu Ala Asn Asn Pro Tyr 
            20                  25                  30          


Ser Ser Ala Leu Asn Leu Asn Ser Cys Gln Asn Ser Ser Ile Leu Asn 
        35                  40                  45              


Trp Ile Asn Ile Ile Gly Asp Ala Ala Lys Glu Ala Val Ser Ile Gly 
    50                  55                  60                  


Thr Thr Ile Val Ser Leu Ile Thr Ala Pro Ser Leu Thr Gly Leu Ile 
65                  70                  75                  80  


Ser Ile Val Tyr Asp Leu Ile Lys Lys Val Leu Gly Gly Ser Ser Gly 
                85                  90                  95      


Gln Ser Ile Ser Asp Leu Ser Ile Cys Asp Leu Leu Ser Ile Ile Asp 
            100                 105                 110         


Leu Arg Val Ser Gln Ser Val Leu Asn Asp Gly Ile Ala Asp Phe Asn 
        115                 120                 125             


Gly Ser Val Leu Leu Tyr Arg Asn Tyr Leu Glu Ala Leu Asp Ser Trp 
    130                 135                 140                 


Asn Lys Asn Pro Asn Ser Ala Ser Ala Glu Glu Leu Arg Thr Arg Phe 
145                 150                 155                 160 


Arg Ile Ala Asp Ser Glu Phe Asp Arg Ile Leu Thr Arg Gly Ser Leu 
                165                 170                 175     


Thr Asn Gly Gly Ser Leu Ala Arg Gln Asn Ala Gln Ile Leu Leu Leu 
            180                 185                 190         


Pro Ser Phe Ala Ser Ala Ala Phe Phe His Leu Leu Leu Leu Arg Asp 
        195                 200                 205             


Ala Thr Arg Tyr Gly Thr Asn Trp Gly Leu Tyr Asn Ala Thr Pro Phe 
    210                 215                 220                 


Ile Asn Tyr Gln Ser Lys Leu Val Glu Leu Ile Glu Leu Tyr Thr Asp 
225                 230                 235                 240 


Tyr Cys Val His Trp Tyr Asn Arg Gly Phe Asn Glu Leu Arg Gln Arg 
                245                 250                 255     


Gly Thr Ser Ala Thr Ala Trp Leu Glu Phe His Arg Tyr Arg Arg Glu 
            260                 265                 270         


Met Thr Leu Met Val Leu Asp Ile Val Ala Ser Phe Ser Ser Leu Asp 
        275                 280                 285             


Ile Thr Asn Tyr Pro Ile Glu Thr Asp Phe Gln Leu Ser Arg Val Ile 
    290                 295                 300                 


Tyr Thr Asp Pro Ile Gly Phe Val His Arg Ser Ser Leu Arg Gly Glu 
305                 310                 315                 320 


Ser Trp Phe Ser Phe Val Asn Arg Ala Asn Phe Ser Asp Leu Glu Asn 
                325                 330                 335     


Ala Ile Pro Asn Pro Arg Pro Ser Trp Phe Leu Asn Asn Met Ile Ile 
            340                 345                 350         


Ser Thr Gly Ser Leu Thr Leu Pro Val Ser Pro Ser Thr Asp Arg Ala 
        355                 360                 365             


Arg Val Trp Tyr Gly Ser Arg Asp Arg Ile Ser Pro Ala Asn Ser Gln 
    370                 375                 380                 


Phe Ile Thr Glu Leu Ile Ser Gly Gln His Thr Thr Ala Thr Gln Thr 
385                 390                 395                 400 


Ile Leu Gly Arg Asn Ile Phe Arg Val Asp Ser Gln Ala Cys Asn Leu 
                405                 410                 415     


Asn Asp Thr Thr Tyr Gly Val Asn Arg Ala Val Phe Tyr His Asp Ala 
            420                 425                 430         


Ser Glu Gly Ser Gln Arg Ser Val Tyr Glu Gly Tyr Ile Arg Thr Thr 
        435                 440                 445             


Gly Ile Asp Asn Pro Arg Val Gln Asn Ile Asn Thr Tyr Leu Pro Gly 
    450                 455                 460                 


Glu Asn Ser Asp Ile Pro Thr Pro Glu Asp Tyr Thr His Ile Leu Ser 
465                 470                 475                 480 


Thr Thr Ile Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Ser Asn Arg 
                485                 490                 495     


Arg Ser Ser Leu Val Met Tyr Gly Trp Thr His Lys Ser Leu Ala Arg 
            500                 505                 510         


Asn Asn Thr Ile Asn Pro Asp Arg Ile Thr Gln Ile Pro Leu Thr Lys 
        515                 520                 525             


Val Asp Thr Arg Gly Thr Gly Val Ser Tyr Val Asn Asp Pro Gly Phe 
    530                 535                 540                 


Ile Gly Gly Ala Leu Leu Gln Arg Thr Asp His Gly Ser Leu Gly Val 
545                 550                 555                 560 


Leu Arg Val Gln Phe Pro Leu His Leu Arg Gln Gln Tyr Arg Ile Arg 
                565                 570                 575     


Val Arg Tyr Ala Ser Thr Thr Asn Ile Arg Leu Ser Val Asn Gly Ser 
            580                 585                 590         


Phe Gly Thr Ile Ser Gln Asn Leu Pro Ser Thr Met Arg Leu Gly Glu 
        595                 600                 605             


Asp Leu Arg Tyr Gly Ser Phe Ala Ile Arg Glu Phe Ser Thr Ser Ile 
    610                 615                 620                 


Arg Pro Thr Ala Ser Pro Asp Gln Ile Arg Leu Thr Ile Glu Pro Ser 
625                 630                 635                 640 


Phe Ile Arg Gln Glu Val Tyr Val Asp Arg Ile Glu Phe Ile Pro Val 
                645                 650                 655     


Asn 
    


<210>  15
<211>  3474
<212>  DNA
<213>  Artificial

<220>
<223>  Plant synthetic coding sequence encoding a TIC4064_12_1 
       pesticidal protein designed for expression in a plant cell 
       wherein an additional alanine codon is inserted and comprising 
       mutations at D85A, S95T, A511H, N513D, and R605N.

<400>  15
atggctaacc agaacaagca cggcatcatt ggcgcatcca actgcgggtg taccagtgac       60

aacgtggcga aatacccgct cgccaataac ccatactcat cagccctgaa cctcaacagt      120

tgtcagaaca gctccatcct gaattggatc aacatcatcg gcgacgcggc gaaggaggcg      180

gtgagcatcg ggacaaccat cgtttcgctt atcacagctc cctcgctgac gggcctcatc      240

tcaatcgttt atgcactcat tgggaaggtg ctcggcggct cgaccggcca atccattagc      300

gacctttcca tctgcgatct gctctccata attgacctgc gggtgtcgca gtcggtcctg      360

aacgacggga tagcggactt caacggcagc gtcctcctgt accgcaacta cctggaggca      420

ctggactcct ggaacaagaa tcccaacagc gccagcgcgg aggagctgcg gacgcgcttt      480

cgcatcgctg attctgagtt tgaccgaatc ctgacacgcg gctcccttac caacggcggt      540

agcctggctc gccagaacgc ccagattctg ttgctgccca gtttcgcgtc agccgcattc      600

tttcacctac ttctccttcg ggacgcaacg cgctacggga ccaattgggg attgtacaac      660

gcgacgccct tcatcaacta tcagtctaag ctcgtcgaac tgatcgaact ctacacggac      720

tactgcgtcc actggtacaa tcgtggcttt aacgagttgc gtcaacgcgg aacttctgct      780

acggcgtggc ttgagtttca cagataccgt cgtgagatga cgctaatggt cctcgacatc      840

gtggcgtcgt tcagctcgct tgacatcacc aactacccta tcgagaccga ctttcaactt      900

agccgtgtga tctacactga cccaatcggc ttcgtccatc gaagttcact ccgtggcgag      960

tcctggttct cgttcgtgaa ccgcgccaac ttcagtgacc ttgagaatgc gatccctaac     1020

ccacgcccta gctggttcct gaacaacatg attatctcta cagggagcct gaccttaccc     1080

gtcagtccca gcacagatcg cgcgagagtc tggtacggga gtcgggaccg gatctcgccc     1140

gccaactctc agttcattac tgagcttatc tccgggcagc acaccaccgc cacccagaca     1200

atactcggtc gcaacatctt ccgcgtggat tcacaggcgt gcaacctaaa cgacaccacg     1260

tacggcgtca accgcgccgt gttctaccat gacgcctcgg agggctcgca gcgtagcgtt     1320

tatgagggct acatcaggac gacgggtatc gacaacccga gagtgcagaa catcaacacc     1380

tatttgccag gcgagaacag cgacatcccg acgcccgaag actacaccca catcctcagc     1440

acaaccatca acctcacggg cgggctccgc caagttgcct cgaaccggcg gagcagcctc     1500

gttatgtacg gctggaccca caagtctctg catcgcgata acaccatcaa tccagatcga     1560

atcacccaga taccactcac gaaggtggat acccgaggga ctggcgttag ttacgtcaac     1620

gatcctggat tcatcggcgg cgcgcttctc caacgcacgg accacgggtc gctgggcgtc     1680

cttcgggtac agtttccgct acacttgcgc cagcaatacc ggataagggt tcggtacgcc     1740

tcaacaacga acatcagact gtctgtcaac ggctccttcg gcaccatctc ccagaacctt     1800

ccctccacca tgcgcctcgg tgaggctctg cgctacggct ctttcgctat ccgcgagttt     1860

agcacttcaa tacgcccaac cgcgtcacct gaccagatac gcctgaccat tgaaccgtcc     1920

ttcatccggc aagaggtgta cgtggacaga atcgagttca taccagtcaa cccgacgcgg     1980

gaggcgaagg aagaccttga ggctaccaag aaggcggtgg cgagcctgtt tacccgcact     2040

cgtgacggcc tccaagtaaa tgtcacggac taccaagtgg accaggcggc caacctcgta     2100

agctgcctat cagacgagca gtacgcccac gacaagaaga tgcttctgga agccgtcaga     2160

gccgccaaac ggctatcgcg cgagcgcaac ctgctccaag atcccgactt caacactatc     2220

aacagcaccg aggagaatgg ctggaaagcc agcaacggcg tgacgattag cgagggcggc     2280

ccgttctaca agggccgcgc actacagctt gcctccgcaa gggagaacta ccctacctac     2340

atctaccaga aggtcgatgc gtcggaactt aaaccataca cacggtatcg tctcgacggg     2400

ttcgtcaaat ccagccaaga cctagagatc gacctcatcc accatcacaa agtacatctc     2460

gtcaagaatg cgcccgacaa tcttgtctct gacacttacc cggacgattc ctgttcgggt     2520

attaacagat gccaggagca gcagatggtg aacgcccaac tcgaaaccga gcaccatcac     2580

ccaatggact gctgcggtgc ggctcagacc cacgagttta gcagttacat cgacaccggc     2640

gacctgaact cgacggtgga ccagggcatc tgggcgatct tcaaggttcg taccaccgac     2700

ggctacgcga ctctcgggaa tctggaactg gtcgagatcg gcccgttgag cggcgagtcg     2760

ctggagcggg agcagaggga caacgcaaag tggtctgccg agctgggccg aaagcgagcg     2820

gagactgacc gcgtgtacca ggatgctaag caaagtatca accacctctt cgtggactat     2880

caagatcaac agctcaaccc tgagatcggg atggctgaca tcatggacgc gcagaacctg     2940

gtggcgtcga tctccgatgt gtactccgac gccgtgctcc aaatccctgg cattaactac     3000

gagatctaca cggagctgtc gaaccgcctc caacaagcga gctaccttta cacgagccgg     3060

aacgccgtac agaacggcga cttcaactcc gggctcgact catggaacgc cactgcgggc     3120

gctaccgtcc aacaagacgg caacacccac tttctcgtcc ttagccactg ggacgcccaa     3180

gtctcgcagc agtttcgcgt tcagccgaac tgcaagtatg tcctgcgcgt gaccgccgag     3240

aaggtgggcg gtggtgacgg ctacgtcacc ataagggatg gtgcccacca cacggagacc     3300

ttaacgttta acgcctgtga ttatgacatc aacggcacct atgtgacgga caatacctac     3360

ctcactaagg aagtcgtgtt ctattcccat accgagcaca tgtgggtcga ggtttcggag     3420

acggagggcg tattccacat cgacagtgtg gagttcatgg agacccagca gtga           3474


<210>  16
<211>  1157
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of TIC4064_12_1 with an additional alanine 
       amino acid inserted immediately following the initiating 
       methionine and comprising mutations at D85A, S95T, A511H, N513D, 
       and  R605N.

<400>  16

Met Ala Asn Gln Asn Lys His Gly Ile Ile Gly Ala Ser Asn Cys Gly 
1               5                   10                  15      


Cys Thr Ser Asp Asn Val Ala Lys Tyr Pro Leu Ala Asn Asn Pro Tyr 
            20                  25                  30          


Ser Ser Ala Leu Asn Leu Asn Ser Cys Gln Asn Ser Ser Ile Leu Asn 
        35                  40                  45              


Trp Ile Asn Ile Ile Gly Asp Ala Ala Lys Glu Ala Val Ser Ile Gly 
    50                  55                  60                  


Thr Thr Ile Val Ser Leu Ile Thr Ala Pro Ser Leu Thr Gly Leu Ile 
65                  70                  75                  80  


Ser Ile Val Tyr Ala Leu Ile Gly Lys Val Leu Gly Gly Ser Thr Gly 
                85                  90                  95      


Gln Ser Ile Ser Asp Leu Ser Ile Cys Asp Leu Leu Ser Ile Ile Asp 
            100                 105                 110         


Leu Arg Val Ser Gln Ser Val Leu Asn Asp Gly Ile Ala Asp Phe Asn 
        115                 120                 125             


Gly Ser Val Leu Leu Tyr Arg Asn Tyr Leu Glu Ala Leu Asp Ser Trp 
    130                 135                 140                 


Asn Lys Asn Pro Asn Ser Ala Ser Ala Glu Glu Leu Arg Thr Arg Phe 
145                 150                 155                 160 


Arg Ile Ala Asp Ser Glu Phe Asp Arg Ile Leu Thr Arg Gly Ser Leu 
                165                 170                 175     


Thr Asn Gly Gly Ser Leu Ala Arg Gln Asn Ala Gln Ile Leu Leu Leu 
            180                 185                 190         


Pro Ser Phe Ala Ser Ala Ala Phe Phe His Leu Leu Leu Leu Arg Asp 
        195                 200                 205             


Ala Thr Arg Tyr Gly Thr Asn Trp Gly Leu Tyr Asn Ala Thr Pro Phe 
    210                 215                 220                 


Ile Asn Tyr Gln Ser Lys Leu Val Glu Leu Ile Glu Leu Tyr Thr Asp 
225                 230                 235                 240 


Tyr Cys Val His Trp Tyr Asn Arg Gly Phe Asn Glu Leu Arg Gln Arg 
                245                 250                 255     


Gly Thr Ser Ala Thr Ala Trp Leu Glu Phe His Arg Tyr Arg Arg Glu 
            260                 265                 270         


Met Thr Leu Met Val Leu Asp Ile Val Ala Ser Phe Ser Ser Leu Asp 
        275                 280                 285             


Ile Thr Asn Tyr Pro Ile Glu Thr Asp Phe Gln Leu Ser Arg Val Ile 
    290                 295                 300                 


Tyr Thr Asp Pro Ile Gly Phe Val His Arg Ser Ser Leu Arg Gly Glu 
305                 310                 315                 320 


Ser Trp Phe Ser Phe Val Asn Arg Ala Asn Phe Ser Asp Leu Glu Asn 
                325                 330                 335     


Ala Ile Pro Asn Pro Arg Pro Ser Trp Phe Leu Asn Asn Met Ile Ile 
            340                 345                 350         


Ser Thr Gly Ser Leu Thr Leu Pro Val Ser Pro Ser Thr Asp Arg Ala 
        355                 360                 365             


Arg Val Trp Tyr Gly Ser Arg Asp Arg Ile Ser Pro Ala Asn Ser Gln 
    370                 375                 380                 


Phe Ile Thr Glu Leu Ile Ser Gly Gln His Thr Thr Ala Thr Gln Thr 
385                 390                 395                 400 


Ile Leu Gly Arg Asn Ile Phe Arg Val Asp Ser Gln Ala Cys Asn Leu 
                405                 410                 415     


Asn Asp Thr Thr Tyr Gly Val Asn Arg Ala Val Phe Tyr His Asp Ala 
            420                 425                 430         


Ser Glu Gly Ser Gln Arg Ser Val Tyr Glu Gly Tyr Ile Arg Thr Thr 
        435                 440                 445             


Gly Ile Asp Asn Pro Arg Val Gln Asn Ile Asn Thr Tyr Leu Pro Gly 
    450                 455                 460                 


Glu Asn Ser Asp Ile Pro Thr Pro Glu Asp Tyr Thr His Ile Leu Ser 
465                 470                 475                 480 


Thr Thr Ile Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Ser Asn Arg 
                485                 490                 495     


Arg Ser Ser Leu Val Met Tyr Gly Trp Thr His Lys Ser Leu His Arg 
            500                 505                 510         


Asp Asn Thr Ile Asn Pro Asp Arg Ile Thr Gln Ile Pro Leu Thr Lys 
        515                 520                 525             


Val Asp Thr Arg Gly Thr Gly Val Ser Tyr Val Asn Asp Pro Gly Phe 
    530                 535                 540                 


Ile Gly Gly Ala Leu Leu Gln Arg Thr Asp His Gly Ser Leu Gly Val 
545                 550                 555                 560 


Leu Arg Val Gln Phe Pro Leu His Leu Arg Gln Gln Tyr Arg Ile Arg 
                565                 570                 575     


Val Arg Tyr Ala Ser Thr Thr Asn Ile Arg Leu Ser Val Asn Gly Ser 
            580                 585                 590         


Phe Gly Thr Ile Ser Gln Asn Leu Pro Ser Thr Met Arg Leu Gly Glu 
        595                 600                 605             


Ala Leu Arg Tyr Gly Ser Phe Ala Ile Arg Glu Phe Ser Thr Ser Ile 
    610                 615                 620                 


Arg Pro Thr Ala Ser Pro Asp Gln Ile Arg Leu Thr Ile Glu Pro Ser 
625                 630                 635                 640 


Phe Ile Arg Gln Glu Val Tyr Val Asp Arg Ile Glu Phe Ile Pro Val 
                645                 650                 655     


Asn Pro Thr Arg Glu Ala Lys Glu Asp Leu Glu Ala Thr Lys Lys Ala 
            660                 665                 670         


Val Ala Ser Leu Phe Thr Arg Thr Arg Asp Gly Leu Gln Val Asn Val 
        675                 680                 685             


Thr Asp Tyr Gln Val Asp Gln Ala Ala Asn Leu Val Ser Cys Leu Ser 
    690                 695                 700                 


Asp Glu Gln Tyr Ala His Asp Lys Lys Met Leu Leu Glu Ala Val Arg 
705                 710                 715                 720 


Ala Ala Lys Arg Leu Ser Arg Glu Arg Asn Leu Leu Gln Asp Pro Asp 
                725                 730                 735     


Phe Asn Thr Ile Asn Ser Thr Glu Glu Asn Gly Trp Lys Ala Ser Asn 
            740                 745                 750         


Gly Val Thr Ile Ser Glu Gly Gly Pro Phe Tyr Lys Gly Arg Ala Leu 
        755                 760                 765             


Gln Leu Ala Ser Ala Arg Glu Asn Tyr Pro Thr Tyr Ile Tyr Gln Lys 
    770                 775                 780                 


Val Asp Ala Ser Glu Leu Lys Pro Tyr Thr Arg Tyr Arg Leu Asp Gly 
785                 790                 795                 800 


Phe Val Lys Ser Ser Gln Asp Leu Glu Ile Asp Leu Ile His His His 
                805                 810                 815     


Lys Val His Leu Val Lys Asn Ala Pro Asp Asn Leu Val Ser Asp Thr 
            820                 825                 830         


Tyr Pro Asp Asp Ser Cys Ser Gly Ile Asn Arg Cys Gln Glu Gln Gln 
        835                 840                 845             


Met Val Asn Ala Gln Leu Glu Thr Glu His His His Pro Met Asp Cys 
    850                 855                 860                 


Cys Gly Ala Ala Gln Thr His Glu Phe Ser Ser Tyr Ile Asp Thr Gly 
865                 870                 875                 880 


Asp Leu Asn Ser Thr Val Asp Gln Gly Ile Trp Ala Ile Phe Lys Val 
                885                 890                 895     


Arg Thr Thr Asp Gly Tyr Ala Thr Leu Gly Asn Leu Glu Leu Val Glu 
            900                 905                 910         


Ile Gly Pro Leu Ser Gly Glu Ser Leu Glu Arg Glu Gln Arg Asp Asn 
        915                 920                 925             


Ala Lys Trp Ser Ala Glu Leu Gly Arg Lys Arg Ala Glu Thr Asp Arg 
    930                 935                 940                 


Val Tyr Gln Asp Ala Lys Gln Ser Ile Asn His Leu Phe Val Asp Tyr 
945                 950                 955                 960 


Gln Asp Gln Gln Leu Asn Pro Glu Ile Gly Met Ala Asp Ile Met Asp 
                965                 970                 975     


Ala Gln Asn Leu Val Ala Ser Ile Ser Asp Val Tyr Ser Asp Ala Val 
            980                 985                 990         


Leu Gln Ile Pro Gly Ile Asn Tyr  Glu Ile Tyr Thr Glu  Leu Ser Asn 
        995                 1000                 1005             


Arg Leu  Gln Gln Ala Ser Tyr  Leu Tyr Thr Ser Arg  Asn Ala Val 
    1010                 1015                 1020             


Gln Asn  Gly Asp Phe Asn Ser  Gly Leu Asp Ser Trp  Asn Ala Thr 
    1025                 1030                 1035             


Ala Gly  Ala Thr Val Gln Gln  Asp Gly Asn Thr His  Phe Leu Val 
    1040                 1045                 1050             


Leu Ser  His Trp Asp Ala Gln  Val Ser Gln Gln Phe  Arg Val Gln 
    1055                 1060                 1065             


Pro Asn  Cys Lys Tyr Val Leu  Arg Val Thr Ala Glu  Lys Val Gly 
    1070                 1075                 1080             


Gly Gly  Asp Gly Tyr Val Thr  Ile Arg Asp Gly Ala  His His Thr 
    1085                 1090                 1095             


Glu Thr  Leu Thr Phe Asn Ala  Cys Asp Tyr Asp Ile  Asn Gly Thr 
    1100                 1105                 1110             


Tyr Val  Thr Asp Asn Thr Tyr  Leu Thr Lys Glu Val  Val Phe Tyr 
    1115                 1120                 1125             


Ser His  Thr Glu His Met Trp  Val Glu Val Ser Glu  Thr Glu Gly 
    1130                 1135                 1140             


Val Phe  His Ile Asp Ser Val  Glu Phe Met Glu Thr  Gln Gln 
    1145                 1150                 1155         


<210>  17
<211>  3474
<212>  DNA
<213>  Artificial

<220>
<223>  Plant synthetic coding sequence encoding a TIC4064_12_2 
       pesticidal protein designed for expression in a plant cell with 
       an additional alanine codon inserted immediately following the 
       initiating methionine and comprising mutations at D85A and S95T.

<400>  17
atggctaacc agaacaagca cggcatcatt ggcgcatcca actgcgggtg taccagtgac       60

aacgtggcga aatacccgct cgccaataac ccatactcat cagccctgaa cctcaacagt      120

tgtcagaaca gctccatcct gaattggatc aacatcatcg gcgacgcggc gaaggaggcg      180

gtgagcatcg ggacaaccat cgtttcgctt atcacagctc cctcgctgac gggcctcatc      240

tcaatcgttt atgcactcat tgggaaggtg ctcggcggct cgaccggcca atccattagc      300

gacctttcca tctgcgatct gctctccata attgacctgc gggtgtcgca gtcggtcctg      360

aacgacggga tagcggactt caacggcagc gtcctcctgt accgcaacta cctggaggca      420

ctggactcct ggaacaagaa tcccaacagc gccagcgcgg aggagctgcg gacgcgcttt      480

cgcatcgctg attctgagtt tgaccgaatc ctgacacgcg gctcccttac caacggcggt      540

agcctggctc gccagaacgc ccagattctg ttgctgccca gtttcgcgtc agccgcattc      600

tttcacctac ttctccttcg ggacgcaacg cgctacggga ccaattgggg attgtacaac      660

gcgacgccct tcatcaacta tcagtctaag ctcgtcgaac tgatcgaact ctacacggac      720

tactgcgtcc actggtacaa tcgtggcttt aacgagttgc gtcaacgcgg aacttctgct      780

acggcgtggc ttgagtttca cagataccgt cgtgagatga cgctaatggt cctcgacatc      840

gtggcgtcgt tcagctcgct tgacatcacc aactacccta tcgagaccga ctttcaactt      900

agccgtgtga tctacactga cccaatcggc ttcgtccatc gaagttcact ccgtggcgag      960

tcctggttct cgttcgtgaa ccgcgccaac ttcagtgacc ttgagaatgc gatccctaac     1020

ccacgcccta gctggttcct gaacaacatg attatctcta cagggagcct gaccttaccc     1080

gtcagtccca gcacagatcg cgcgagagtc tggtacggga gtcgggaccg gatctcgccc     1140

gccaactctc agttcattac tgagcttatc tccgggcagc acaccaccgc cacccagaca     1200

atactcggtc gcaacatctt ccgcgtggat tcacaggcgt gcaacctaaa cgacaccacg     1260

tacggcgtca accgcgccgt gttctaccat gacgcctcgg agggctcgca gcgtagcgtt     1320

tatgagggct acatcaggac gacgggtatc gacaacccga gagtgcagaa catcaacacc     1380

tatttgccag gcgagaacag cgacatcccg acgcccgaag actacaccca catcctcagc     1440

acaaccatca acctcacggg cgggctccgc caagttgcct cgaaccggcg gagcagcctc     1500

gttatgtacg gctggaccca caagtctctg gcgcgcaaca acaccatcaa tccagatcga     1560

atcacccaga taccactcac gaaggtggat acccgaggga ctggcgttag ttacgtcaac     1620

gatcctggat tcatcggcgg cgcgcttctc caacgcacgg accacgggtc gctgggcgtc     1680

cttcgggtac agtttccgct acacttgcgc cagcaatacc ggataagggt tcggtacgcc     1740

tcaacaacga acatcagact gtctgtcaac ggctccttcg gcaccatctc ccagaacctt     1800

ccctccacca tgcgcctcgg tgaggatctg cgctacggct ctttcgctat ccgcgagttt     1860

agcacttcaa tacgcccaac cgcgtcacct gaccagatac gcctgaccat tgaaccgtcc     1920

ttcatccggc aagaggtgta cgtggacaga atcgagttca taccagtcaa cccgacgcgg     1980

gaggcgaagg aagaccttga ggctaccaag aaggcggtgg cgagcctgtt tacccgcact     2040

cgtgacggcc tccaagtaaa tgtcacggac taccaagtgg accaggcggc caacctcgta     2100

agctgcctat cagacgagca gtacgcccac gacaagaaga tgcttctgga agccgtcaga     2160

gccgccaaac ggctatcgcg cgagcgcaac ctgctccaag atcccgactt caacactatc     2220

aacagcaccg aggagaatgg ctggaaagcc agcaacggcg tgacgattag cgagggcggc     2280

ccgttctaca agggccgcgc actacagctt gcctccgcaa gggagaacta ccctacctac     2340

atctaccaga aggtcgatgc gtcggaactt aaaccataca cacggtatcg tctcgacggg     2400

ttcgtcaaat ccagccaaga cctagagatc gacctcatcc accatcacaa agtacatctc     2460

gtcaagaatg cgcccgacaa tcttgtctct gacacttacc cggacgattc ctgttcgggt     2520

attaacagat gccaggagca gcagatggtg aacgcccaac tcgaaaccga gcaccatcac     2580

ccaatggact gctgcggtgc ggctcagacc cacgagttta gcagttacat cgacaccggc     2640

gacctgaact cgacggtgga ccagggcatc tgggcgatct tcaaggttcg taccaccgac     2700

ggctacgcga ctctcgggaa tctggaactg gtcgagatcg gcccgttgag cggcgagtcg     2760

ctggagcggg agcagaggga caacgcaaag tggtctgccg agctgggccg aaagcgagcg     2820

gagactgacc gcgtgtacca ggatgctaag caaagtatca accacctctt cgtggactat     2880

caagatcaac agctcaaccc tgagatcggg atggctgaca tcatggacgc gcagaacctg     2940

gtggcgtcga tctccgatgt gtactccgac gccgtgctcc aaatccctgg cattaactac     3000

gagatctaca cggagctgtc gaaccgcctc caacaagcga gctaccttta cacgagccgg     3060

aacgccgtac agaacggcga cttcaactcc gggctcgact catggaacgc cactgcgggc     3120

gctaccgtcc aacaagacgg caacacccac tttctcgtcc ttagccactg ggacgcccaa     3180

gtctcgcagc agtttcgcgt tcagccgaac tgcaagtatg tcctgcgcgt gaccgccgag     3240

aaggtgggcg gtggtgacgg ctacgtcacc ataagggatg gtgcccacca cacggagacc     3300

ttaacgttta acgcctgtga ttatgacatc aacggcacct atgtgacgga caatacctac     3360

ctcactaagg aagtcgtgtt ctattcccat accgagcaca tgtgggtcga ggtttcggag     3420

acggagggcg tattccacat cgacagtgtg gagttcatgg agacccagca gtga           3474


<210>  18
<211>  1157
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of TIC4064_12_2  wherein an additional 
       alanine amino acid is inserted immediately following the 
       initiating methionine and comprising mutations at D85A and S95T.

<400>  18

Met Ala Asn Gln Asn Lys His Gly Ile Ile Gly Ala Ser Asn Cys Gly 
1               5                   10                  15      


Cys Thr Ser Asp Asn Val Ala Lys Tyr Pro Leu Ala Asn Asn Pro Tyr 
            20                  25                  30          


Ser Ser Ala Leu Asn Leu Asn Ser Cys Gln Asn Ser Ser Ile Leu Asn 
        35                  40                  45              


Trp Ile Asn Ile Ile Gly Asp Ala Ala Lys Glu Ala Val Ser Ile Gly 
    50                  55                  60                  


Thr Thr Ile Val Ser Leu Ile Thr Ala Pro Ser Leu Thr Gly Leu Ile 
65                  70                  75                  80  


Ser Ile Val Tyr Ala Leu Ile Gly Lys Val Leu Gly Gly Ser Thr Gly 
                85                  90                  95      


Gln Ser Ile Ser Asp Leu Ser Ile Cys Asp Leu Leu Ser Ile Ile Asp 
            100                 105                 110         


Leu Arg Val Ser Gln Ser Val Leu Asn Asp Gly Ile Ala Asp Phe Asn 
        115                 120                 125             


Gly Ser Val Leu Leu Tyr Arg Asn Tyr Leu Glu Ala Leu Asp Ser Trp 
    130                 135                 140                 


Asn Lys Asn Pro Asn Ser Ala Ser Ala Glu Glu Leu Arg Thr Arg Phe 
145                 150                 155                 160 


Arg Ile Ala Asp Ser Glu Phe Asp Arg Ile Leu Thr Arg Gly Ser Leu 
                165                 170                 175     


Thr Asn Gly Gly Ser Leu Ala Arg Gln Asn Ala Gln Ile Leu Leu Leu 
            180                 185                 190         


Pro Ser Phe Ala Ser Ala Ala Phe Phe His Leu Leu Leu Leu Arg Asp 
        195                 200                 205             


Ala Thr Arg Tyr Gly Thr Asn Trp Gly Leu Tyr Asn Ala Thr Pro Phe 
    210                 215                 220                 


Ile Asn Tyr Gln Ser Lys Leu Val Glu Leu Ile Glu Leu Tyr Thr Asp 
225                 230                 235                 240 


Tyr Cys Val His Trp Tyr Asn Arg Gly Phe Asn Glu Leu Arg Gln Arg 
                245                 250                 255     


Gly Thr Ser Ala Thr Ala Trp Leu Glu Phe His Arg Tyr Arg Arg Glu 
            260                 265                 270         


Met Thr Leu Met Val Leu Asp Ile Val Ala Ser Phe Ser Ser Leu Asp 
        275                 280                 285             


Ile Thr Asn Tyr Pro Ile Glu Thr Asp Phe Gln Leu Ser Arg Val Ile 
    290                 295                 300                 


Tyr Thr Asp Pro Ile Gly Phe Val His Arg Ser Ser Leu Arg Gly Glu 
305                 310                 315                 320 


Ser Trp Phe Ser Phe Val Asn Arg Ala Asn Phe Ser Asp Leu Glu Asn 
                325                 330                 335     


Ala Ile Pro Asn Pro Arg Pro Ser Trp Phe Leu Asn Asn Met Ile Ile 
            340                 345                 350         


Ser Thr Gly Ser Leu Thr Leu Pro Val Ser Pro Ser Thr Asp Arg Ala 
        355                 360                 365             


Arg Val Trp Tyr Gly Ser Arg Asp Arg Ile Ser Pro Ala Asn Ser Gln 
    370                 375                 380                 


Phe Ile Thr Glu Leu Ile Ser Gly Gln His Thr Thr Ala Thr Gln Thr 
385                 390                 395                 400 


Ile Leu Gly Arg Asn Ile Phe Arg Val Asp Ser Gln Ala Cys Asn Leu 
                405                 410                 415     


Asn Asp Thr Thr Tyr Gly Val Asn Arg Ala Val Phe Tyr His Asp Ala 
            420                 425                 430         


Ser Glu Gly Ser Gln Arg Ser Val Tyr Glu Gly Tyr Ile Arg Thr Thr 
        435                 440                 445             


Gly Ile Asp Asn Pro Arg Val Gln Asn Ile Asn Thr Tyr Leu Pro Gly 
    450                 455                 460                 


Glu Asn Ser Asp Ile Pro Thr Pro Glu Asp Tyr Thr His Ile Leu Ser 
465                 470                 475                 480 


Thr Thr Ile Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Ser Asn Arg 
                485                 490                 495     


Arg Ser Ser Leu Val Met Tyr Gly Trp Thr His Lys Ser Leu Ala Arg 
            500                 505                 510         


Asn Asn Thr Ile Asn Pro Asp Arg Ile Thr Gln Ile Pro Leu Thr Lys 
        515                 520                 525             


Val Asp Thr Arg Gly Thr Gly Val Ser Tyr Val Asn Asp Pro Gly Phe 
    530                 535                 540                 


Ile Gly Gly Ala Leu Leu Gln Arg Thr Asp His Gly Ser Leu Gly Val 
545                 550                 555                 560 


Leu Arg Val Gln Phe Pro Leu His Leu Arg Gln Gln Tyr Arg Ile Arg 
                565                 570                 575     


Val Arg Tyr Ala Ser Thr Thr Asn Ile Arg Leu Ser Val Asn Gly Ser 
            580                 585                 590         


Phe Gly Thr Ile Ser Gln Asn Leu Pro Ser Thr Met Arg Leu Gly Glu 
        595                 600                 605             


Asp Leu Arg Tyr Gly Ser Phe Ala Ile Arg Glu Phe Ser Thr Ser Ile 
    610                 615                 620                 


Arg Pro Thr Ala Ser Pro Asp Gln Ile Arg Leu Thr Ile Glu Pro Ser 
625                 630                 635                 640 


Phe Ile Arg Gln Glu Val Tyr Val Asp Arg Ile Glu Phe Ile Pro Val 
                645                 650                 655     


Asn Pro Thr Arg Glu Ala Lys Glu Asp Leu Glu Ala Thr Lys Lys Ala 
            660                 665                 670         


Val Ala Ser Leu Phe Thr Arg Thr Arg Asp Gly Leu Gln Val Asn Val 
        675                 680                 685             


Thr Asp Tyr Gln Val Asp Gln Ala Ala Asn Leu Val Ser Cys Leu Ser 
    690                 695                 700                 


Asp Glu Gln Tyr Ala His Asp Lys Lys Met Leu Leu Glu Ala Val Arg 
705                 710                 715                 720 


Ala Ala Lys Arg Leu Ser Arg Glu Arg Asn Leu Leu Gln Asp Pro Asp 
                725                 730                 735     


Phe Asn Thr Ile Asn Ser Thr Glu Glu Asn Gly Trp Lys Ala Ser Asn 
            740                 745                 750         


Gly Val Thr Ile Ser Glu Gly Gly Pro Phe Tyr Lys Gly Arg Ala Leu 
        755                 760                 765             


Gln Leu Ala Ser Ala Arg Glu Asn Tyr Pro Thr Tyr Ile Tyr Gln Lys 
    770                 775                 780                 


Val Asp Ala Ser Glu Leu Lys Pro Tyr Thr Arg Tyr Arg Leu Asp Gly 
785                 790                 795                 800 


Phe Val Lys Ser Ser Gln Asp Leu Glu Ile Asp Leu Ile His His His 
                805                 810                 815     


Lys Val His Leu Val Lys Asn Ala Pro Asp Asn Leu Val Ser Asp Thr 
            820                 825                 830         


Tyr Pro Asp Asp Ser Cys Ser Gly Ile Asn Arg Cys Gln Glu Gln Gln 
        835                 840                 845             


Met Val Asn Ala Gln Leu Glu Thr Glu His His His Pro Met Asp Cys 
    850                 855                 860                 


Cys Gly Ala Ala Gln Thr His Glu Phe Ser Ser Tyr Ile Asp Thr Gly 
865                 870                 875                 880 


Asp Leu Asn Ser Thr Val Asp Gln Gly Ile Trp Ala Ile Phe Lys Val 
                885                 890                 895     


Arg Thr Thr Asp Gly Tyr Ala Thr Leu Gly Asn Leu Glu Leu Val Glu 
            900                 905                 910         


Ile Gly Pro Leu Ser Gly Glu Ser Leu Glu Arg Glu Gln Arg Asp Asn 
        915                 920                 925             


Ala Lys Trp Ser Ala Glu Leu Gly Arg Lys Arg Ala Glu Thr Asp Arg 
    930                 935                 940                 


Val Tyr Gln Asp Ala Lys Gln Ser Ile Asn His Leu Phe Val Asp Tyr 
945                 950                 955                 960 


Gln Asp Gln Gln Leu Asn Pro Glu Ile Gly Met Ala Asp Ile Met Asp 
                965                 970                 975     


Ala Gln Asn Leu Val Ala Ser Ile Ser Asp Val Tyr Ser Asp Ala Val 
            980                 985                 990         


Leu Gln Ile Pro Gly Ile Asn Tyr  Glu Ile Tyr Thr Glu  Leu Ser Asn 
        995                 1000                 1005             


Arg Leu  Gln Gln Ala Ser Tyr  Leu Tyr Thr Ser Arg  Asn Ala Val 
    1010                 1015                 1020             


Gln Asn  Gly Asp Phe Asn Ser  Gly Leu Asp Ser Trp  Asn Ala Thr 
    1025                 1030                 1035             


Ala Gly  Ala Thr Val Gln Gln  Asp Gly Asn Thr His  Phe Leu Val 
    1040                 1045                 1050             


Leu Ser  His Trp Asp Ala Gln  Val Ser Gln Gln Phe  Arg Val Gln 
    1055                 1060                 1065             


Pro Asn  Cys Lys Tyr Val Leu  Arg Val Thr Ala Glu  Lys Val Gly 
    1070                 1075                 1080             


Gly Gly  Asp Gly Tyr Val Thr  Ile Arg Asp Gly Ala  His His Thr 
    1085                 1090                 1095             


Glu Thr  Leu Thr Phe Asn Ala  Cys Asp Tyr Asp Ile  Asn Gly Thr 
    1100                 1105                 1110             


Tyr Val  Thr Asp Asn Thr Tyr  Leu Thr Lys Glu Val  Val Phe Tyr 
    1115                 1120                 1125             


Ser His  Thr Glu His Met Trp  Val Glu Val Ser Glu  Thr Glu Gly 
    1130                 1135                 1140             


Val Phe  His Ile Asp Ser Val  Glu Phe Met Glu Thr  Gln Gln 
    1145                 1150                 1155         


<210>  19
<211>  1992
<212>  DNA
<213>  Artificial

<220>
<223>  Plant synthetic coding sequence encoding a TIC4064_13 with an 
       additional alanine codon inserted immediately following the 
       initiating methionine, comprising  mutations at D85A, S95T, 
       A511H, N513D, and R605N and protoxin truncation.

<400>  19
atggctaacc agaacaagca cggcatcatt ggcgcatcca actgcgggtg taccagtgac       60

aacgtggcga aatacccgct cgccaataac ccatactcat cagccctgaa cctcaacagt      120

tgtcagaaca gctccatcct gaattggatc aacatcatcg gcgacgcggc gaaggaggcg      180

gtgagcatcg ggacaaccat cgtttcgctt atcacagctc cctcgctgac gggcctcatc      240

tcaatcgttt atgcactcat tgggaaggtg ctcggcggct cgaccggcca atccattagc      300

gacctttcca tctgcgatct gctctccata attgacctgc gggtgtcgca gtcggtcctg      360

aacgacggga tagcggactt caacggcagc gtcctcctgt accgcaacta cctggaggca      420

ctggactcct ggaacaagaa tcccaacagc gccagcgcgg aggagctgcg gacgcgcttt      480

cgcatcgctg attctgagtt tgaccgaatc ctgacacgcg gctcccttac caacggcggt      540

agcctggctc gccagaacgc ccagattctg ttgctgccca gtttcgcgtc agccgcattc      600

tttcacctac ttctccttcg ggacgcaacg cgctacggga ccaattgggg attgtacaac      660

gcgacgccct tcatcaacta tcagtctaag ctcgtcgaac tgatcgaact ctacacggac      720

tactgcgtcc actggtacaa tcgtggcttt aacgagttgc gtcaacgcgg aacttctgct      780

acggcgtggc ttgagtttca cagataccgt cgtgagatga cgctaatggt cctcgacatc      840

gtggcgtcgt tcagctcgct tgacatcacc aactacccta tcgagaccga ctttcaactt      900

agccgtgtga tctacactga cccaatcggc ttcgtccatc gaagttcact ccgtggcgag      960

tcctggttct cgttcgtgaa ccgcgccaac ttcagtgacc ttgagaatgc gatccctaac     1020

ccacgcccta gctggttcct gaacaacatg attatctcta cagggagcct gaccttaccc     1080

gtcagtccca gcacagatcg cgcgagagtc tggtacggga gtcgggaccg gatctcgccc     1140

gccaactctc agttcattac tgagcttatc tccgggcagc acaccaccgc cacccagaca     1200

atactcggtc gcaacatctt ccgcgtggat tcacaggcgt gcaacctaaa cgacaccacg     1260

tacggcgtca accgcgccgt gttctaccat gacgcctcgg agggctcgca gcgtagcgtt     1320

tatgagggct acatcaggac gacgggtatc gacaacccga gagtgcagaa catcaacacc     1380

tatttgccag gcgagaacag cgacatcccg acgcccgaag actacaccca catcctcagc     1440

acaaccatca acctcacggg cgggctccgc caagttgcct cgaaccggcg gagcagcctc     1500

gttatgtacg gctggaccca caagtctctg catcgcgata acaccatcaa tccagatcga     1560

atcacccaga taccactcac gaaggtggat acccgaggga ctggcgttag ttacgtcaac     1620

gatcctggat tcatcggcgg cgcgcttctc caacgcacgg accacgggtc gctgggcgtc     1680

cttcgggtac agtttccgct acacttgcgc cagcaatacc ggataagggt tcggtacgcc     1740

tcaacaacga acatcagact gtctgtcaac ggctccttcg gcaccatctc ccagaacctt     1800

ccctccacca tgcgcctcgg tgaggctctg cgctacggct ctttcgctat ccgcgagttt     1860

agcacttcaa tacgcccaac cgcgtcacct gaccagatac gcctgaccat tgaaccgtcc     1920

ttcatccggc aagaggtgta cgtggacaga atcgagttca taccagtcaa cccgacgcgg     1980

gaggcgaagt ga                                                         1992


<210>  20
<211>  663
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of TIC4064_13 with an additional alanine 
       amino acid inserted immediately following the initiating 
       methionine, and comprising mutations at D85A, S95T, A511H, N513D,
       and R605N and truncation of the protoxin domain.

<400>  20

Met Ala Asn Gln Asn Lys His Gly Ile Ile Gly Ala Ser Asn Cys Gly 
1               5                   10                  15      


Cys Thr Ser Asp Asn Val Ala Lys Tyr Pro Leu Ala Asn Asn Pro Tyr 
            20                  25                  30          


Ser Ser Ala Leu Asn Leu Asn Ser Cys Gln Asn Ser Ser Ile Leu Asn 
        35                  40                  45              


Trp Ile Asn Ile Ile Gly Asp Ala Ala Lys Glu Ala Val Ser Ile Gly 
    50                  55                  60                  


Thr Thr Ile Val Ser Leu Ile Thr Ala Pro Ser Leu Thr Gly Leu Ile 
65                  70                  75                  80  


Ser Ile Val Tyr Ala Leu Ile Gly Lys Val Leu Gly Gly Ser Thr Gly 
                85                  90                  95      


Gln Ser Ile Ser Asp Leu Ser Ile Cys Asp Leu Leu Ser Ile Ile Asp 
            100                 105                 110         


Leu Arg Val Ser Gln Ser Val Leu Asn Asp Gly Ile Ala Asp Phe Asn 
        115                 120                 125             


Gly Ser Val Leu Leu Tyr Arg Asn Tyr Leu Glu Ala Leu Asp Ser Trp 
    130                 135                 140                 


Asn Lys Asn Pro Asn Ser Ala Ser Ala Glu Glu Leu Arg Thr Arg Phe 
145                 150                 155                 160 


Arg Ile Ala Asp Ser Glu Phe Asp Arg Ile Leu Thr Arg Gly Ser Leu 
                165                 170                 175     


Thr Asn Gly Gly Ser Leu Ala Arg Gln Asn Ala Gln Ile Leu Leu Leu 
            180                 185                 190         


Pro Ser Phe Ala Ser Ala Ala Phe Phe His Leu Leu Leu Leu Arg Asp 
        195                 200                 205             


Ala Thr Arg Tyr Gly Thr Asn Trp Gly Leu Tyr Asn Ala Thr Pro Phe 
    210                 215                 220                 


Ile Asn Tyr Gln Ser Lys Leu Val Glu Leu Ile Glu Leu Tyr Thr Asp 
225                 230                 235                 240 


Tyr Cys Val His Trp Tyr Asn Arg Gly Phe Asn Glu Leu Arg Gln Arg 
                245                 250                 255     


Gly Thr Ser Ala Thr Ala Trp Leu Glu Phe His Arg Tyr Arg Arg Glu 
            260                 265                 270         


Met Thr Leu Met Val Leu Asp Ile Val Ala Ser Phe Ser Ser Leu Asp 
        275                 280                 285             


Ile Thr Asn Tyr Pro Ile Glu Thr Asp Phe Gln Leu Ser Arg Val Ile 
    290                 295                 300                 


Tyr Thr Asp Pro Ile Gly Phe Val His Arg Ser Ser Leu Arg Gly Glu 
305                 310                 315                 320 


Ser Trp Phe Ser Phe Val Asn Arg Ala Asn Phe Ser Asp Leu Glu Asn 
                325                 330                 335     


Ala Ile Pro Asn Pro Arg Pro Ser Trp Phe Leu Asn Asn Met Ile Ile 
            340                 345                 350         


Ser Thr Gly Ser Leu Thr Leu Pro Val Ser Pro Ser Thr Asp Arg Ala 
        355                 360                 365             


Arg Val Trp Tyr Gly Ser Arg Asp Arg Ile Ser Pro Ala Asn Ser Gln 
    370                 375                 380                 


Phe Ile Thr Glu Leu Ile Ser Gly Gln His Thr Thr Ala Thr Gln Thr 
385                 390                 395                 400 


Ile Leu Gly Arg Asn Ile Phe Arg Val Asp Ser Gln Ala Cys Asn Leu 
                405                 410                 415     


Asn Asp Thr Thr Tyr Gly Val Asn Arg Ala Val Phe Tyr His Asp Ala 
            420                 425                 430         


Ser Glu Gly Ser Gln Arg Ser Val Tyr Glu Gly Tyr Ile Arg Thr Thr 
        435                 440                 445             


Gly Ile Asp Asn Pro Arg Val Gln Asn Ile Asn Thr Tyr Leu Pro Gly 
    450                 455                 460                 


Glu Asn Ser Asp Ile Pro Thr Pro Glu Asp Tyr Thr His Ile Leu Ser 
465                 470                 475                 480 


Thr Thr Ile Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Ser Asn Arg 
                485                 490                 495     


Arg Ser Ser Leu Val Met Tyr Gly Trp Thr His Lys Ser Leu His Arg 
            500                 505                 510         


Asp Asn Thr Ile Asn Pro Asp Arg Ile Thr Gln Ile Pro Leu Thr Lys 
        515                 520                 525             


Val Asp Thr Arg Gly Thr Gly Val Ser Tyr Val Asn Asp Pro Gly Phe 
    530                 535                 540                 


Ile Gly Gly Ala Leu Leu Gln Arg Thr Asp His Gly Ser Leu Gly Val 
545                 550                 555                 560 


Leu Arg Val Gln Phe Pro Leu His Leu Arg Gln Gln Tyr Arg Ile Arg 
                565                 570                 575     


Val Arg Tyr Ala Ser Thr Thr Asn Ile Arg Leu Ser Val Asn Gly Ser 
            580                 585                 590         


Phe Gly Thr Ile Ser Gln Asn Leu Pro Ser Thr Met Arg Leu Gly Glu 
        595                 600                 605             


Ala Leu Arg Tyr Gly Ser Phe Ala Ile Arg Glu Phe Ser Thr Ser Ile 
    610                 615                 620                 


Arg Pro Thr Ala Ser Pro Asp Gln Ile Arg Leu Thr Ile Glu Pro Ser 
625                 630                 635                 640 


Phe Ile Arg Gln Glu Val Tyr Val Asp Arg Ile Glu Phe Ile Pro Val 
                645                 650                 655     


Asn Pro Thr Arg Glu Ala Lys 
            660             


<210>  21
<211>  3474
<212>  DNA
<213>  Artificial

<220>
<223>  Plant synthetic coding sequence encoding a TIC4064_14 pesticidal 
       protein with an additional alanine codon inserted immediately 
       following the initiating methionine and comprising mutations at 
       S95T, R169K, and S332A.

<400>  21
atggctaacc agaacaagca cggcatcatt ggcgcatcca actgcgggtg taccagtgac       60

aacgtggcga aatacccgct cgccaataac ccatactcat cagccctgaa cctcaacagt      120

tgtcagaaca gctccatcct gaattggatc aacatcatcg gcgacgcggc gaaggaggcg      180

gtgagcatcg ggacaaccat cgtttcgctt atcacagctc cctcgctgac gggcctcatc      240

tcaatcgttt atgacctcat tgggaaggtg ctcggcggct cgaccggcca atccattagc      300

gacctttcca tctgcgatct gctctccata attgacctgc gggtgtcgca gtcggtcctg      360

aacgacggga tagcggactt caacggcagc gtcctcctgt accgcaacta cctggaggca      420

ctggactcct ggaacaagaa tcccaacagc gccagcgcgg aggagctgcg gacgcgcttt      480

cgcatcgctg attctgagtt tgacaagatc ctgacacgcg gctcccttac caacggcggt      540

agcctggctc gccagaacgc ccagattctg ttgctgccca gtttcgcgtc agccgcattc      600

tttcacctac ttctccttcg ggacgcaacg cgctacggga ccaattgggg attgtacaac      660

gcgacgccct tcatcaacta tcagtctaag ctcgtcgaac tgatcgaact ctacacggac      720

tactgcgtcc actggtacaa tcgtggcttt aacgagttgc gtcaacgcgg aacttctgct      780

acggcgtggc ttgagtttca cagataccgt cgtgagatga cgctaatggt cctcgacatc      840

gtggcgtcgt tcagctcgct tgacatcacc aactacccta tcgagaccga ctttcaactt      900

agccgtgtga tctacactga cccaatcggc ttcgtccatc gaagttcact ccgtggcgag      960

tcctggttct cgttcgtgaa ccgcgccaac ttcgctgacc ttgagaatgc gatccctaac     1020

ccacgcccta gctggttcct gaacaacatg attatctcta cagggagcct gaccttaccc     1080

gtcagtccca gcacagatcg cgcgagagtc tggtacggga gtcgggaccg gatctcgccc     1140

gccaactctc agttcattac tgagcttatc tccgggcagc acaccaccgc cacccagaca     1200

atactcggtc gcaacatctt ccgcgtggat tcacaggcgt gcaacctaaa cgacaccacg     1260

tacggcgtca accgcgccgt gttctaccat gacgcctcgg agggctcgca gcgtagcgtt     1320

tatgagggct acatcaggac gacgggtatc gacaacccga gagtgcagaa catcaacacc     1380

tatttgccag gcgagaacag cgacatcccg acgcccgaag actacaccca catcctcagc     1440

acaaccatca acctcacggg cgggctccgc caagttgcct cgaaccggcg gagcagcctc     1500

gttatgtacg gctggaccca caagtctctg gcgcgcaaca acaccatcaa tccagatcga     1560

atcacccaga taccactcac gaaggtggat acccgaggga ctggcgttag ttacgtcaac     1620

gatcctggat tcatcggcgg cgcgcttctc caacgcacgg accacgggtc gctgggcgtc     1680

cttcgggtac agtttccgct acacttgcgc cagcaatacc ggataagggt tcggtacgcc     1740

tcaacaacga acatcagact gtctgtcaac ggctccttcg gcaccatctc ccagaacctt     1800

ccctccacca tgcgcctcgg tgaggatctg cgctacggct ctttcgctat ccgcgagttt     1860

agcacttcaa tacgcccaac cgcgtcacct gaccagatac gcctgaccat tgaaccgtcc     1920

ttcatccggc aagaggtgta cgtggacaga atcgagttca taccagtcaa cccgacgcgg     1980

gaggcgaagg aagaccttga ggctaccaag aaggcggtgg cgagcctgtt tacccgcact     2040

cgtgacggcc tccaagtaaa tgtcacggac taccaagtgg accaggcggc caacctcgta     2100

agctgcctat cagacgagca gtacgcccac gacaagaaga tgcttctgga agccgtcaga     2160

gccgccaaac ggctatcgcg cgagcgcaac ctgctccaag atcccgactt caacactatc     2220

aacagcaccg aggagaatgg ctggaaagcc agcaacggcg tgacgattag cgagggcggc     2280

ccgttctaca agggccgcgc actacagctt gcctccgcaa gggagaacta ccctacctac     2340

atctaccaga aggtcgatgc gtcggaactt aaaccataca cacggtatcg tctcgacggg     2400

ttcgtcaaat ccagccaaga cctagagatc gacctcatcc accatcacaa agtacatctc     2460

gtcaagaatg cgcccgacaa tcttgtctct gacacttacc cggacgattc ctgttcgggt     2520

attaacagat gccaggagca gcagatggtg aacgcccaac tcgaaaccga gcaccatcac     2580

ccaatggact gctgcggtgc ggctcagacc cacgagttta gcagttacat cgacaccggc     2640

gacctgaact cgacggtgga ccagggcatc tgggcgatct tcaaggttcg taccaccgac     2700

ggctacgcga ctctcgggaa tctggaactg gtcgagatcg gcccgttgag cggcgagtcg     2760

ctggagcggg agcagaggga caacgcaaag tggtctgccg agctgggccg aaagcgagcg     2820

gagactgacc gcgtgtacca ggatgctaag caaagtatca accacctctt cgtggactat     2880

caagatcaac agctcaaccc tgagatcggg atggctgaca tcatggacgc gcagaacctg     2940

gtggcgtcga tctccgatgt gtactccgac gccgtgctcc aaatccctgg cattaactac     3000

gagatctaca cggagctgtc gaaccgcctc caacaagcga gctaccttta cacgagccgg     3060

aacgccgtac agaacggcga cttcaactcc gggctcgact catggaacgc cactgcgggc     3120

gctaccgtcc aacaagacgg caacacccac tttctcgtcc ttagccactg ggacgcccaa     3180

gtctcgcagc agtttcgcgt tcagccgaac tgcaagtatg tcctgcgcgt gaccgccgag     3240

aaggtgggcg gtggtgacgg ctacgtcacc ataagggatg gtgcccacca cacggagacc     3300

ttaacgttta acgcctgtga ttatgacatc aacggcacct atgtgacgga caatacctac     3360

ctcactaagg aagtcgtgtt ctattcccat accgagcaca tgtgggtcga ggtttcggag     3420

acggagggcg tattccacat cgacagtgtg gagttcatgg agacccagca gtga           3474


<210>  22
<211>  1157
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of TIC4064_14 with an additional alanine 
       amino acid inserted immediately following the initiating 
       methionine and comprising mutations at S95T, R169K, and S332A.

<400>  22

Met Ala Asn Gln Asn Lys His Gly Ile Ile Gly Ala Ser Asn Cys Gly 
1               5                   10                  15      


Cys Thr Ser Asp Asn Val Ala Lys Tyr Pro Leu Ala Asn Asn Pro Tyr 
            20                  25                  30          


Ser Ser Ala Leu Asn Leu Asn Ser Cys Gln Asn Ser Ser Ile Leu Asn 
        35                  40                  45              


Trp Ile Asn Ile Ile Gly Asp Ala Ala Lys Glu Ala Val Ser Ile Gly 
    50                  55                  60                  


Thr Thr Ile Val Ser Leu Ile Thr Ala Pro Ser Leu Thr Gly Leu Ile 
65                  70                  75                  80  


Ser Ile Val Tyr Asp Leu Ile Gly Lys Val Leu Gly Gly Ser Thr Gly 
                85                  90                  95      


Gln Ser Ile Ser Asp Leu Ser Ile Cys Asp Leu Leu Ser Ile Ile Asp 
            100                 105                 110         


Leu Arg Val Ser Gln Ser Val Leu Asn Asp Gly Ile Ala Asp Phe Asn 
        115                 120                 125             


Gly Ser Val Leu Leu Tyr Arg Asn Tyr Leu Glu Ala Leu Asp Ser Trp 
    130                 135                 140                 


Asn Lys Asn Pro Asn Ser Ala Ser Ala Glu Glu Leu Arg Thr Arg Phe 
145                 150                 155                 160 


Arg Ile Ala Asp Ser Glu Phe Asp Lys Ile Leu Thr Arg Gly Ser Leu 
                165                 170                 175     


Thr Asn Gly Gly Ser Leu Ala Arg Gln Asn Ala Gln Ile Leu Leu Leu 
            180                 185                 190         


Pro Ser Phe Ala Ser Ala Ala Phe Phe His Leu Leu Leu Leu Arg Asp 
        195                 200                 205             


Ala Thr Arg Tyr Gly Thr Asn Trp Gly Leu Tyr Asn Ala Thr Pro Phe 
    210                 215                 220                 


Ile Asn Tyr Gln Ser Lys Leu Val Glu Leu Ile Glu Leu Tyr Thr Asp 
225                 230                 235                 240 


Tyr Cys Val His Trp Tyr Asn Arg Gly Phe Asn Glu Leu Arg Gln Arg 
                245                 250                 255     


Gly Thr Ser Ala Thr Ala Trp Leu Glu Phe His Arg Tyr Arg Arg Glu 
            260                 265                 270         


Met Thr Leu Met Val Leu Asp Ile Val Ala Ser Phe Ser Ser Leu Asp 
        275                 280                 285             


Ile Thr Asn Tyr Pro Ile Glu Thr Asp Phe Gln Leu Ser Arg Val Ile 
    290                 295                 300                 


Tyr Thr Asp Pro Ile Gly Phe Val His Arg Ser Ser Leu Arg Gly Glu 
305                 310                 315                 320 


Ser Trp Phe Ser Phe Val Asn Arg Ala Asn Phe Ala Asp Leu Glu Asn 
                325                 330                 335     


Ala Ile Pro Asn Pro Arg Pro Ser Trp Phe Leu Asn Asn Met Ile Ile 
            340                 345                 350         


Ser Thr Gly Ser Leu Thr Leu Pro Val Ser Pro Ser Thr Asp Arg Ala 
        355                 360                 365             


Arg Val Trp Tyr Gly Ser Arg Asp Arg Ile Ser Pro Ala Asn Ser Gln 
    370                 375                 380                 


Phe Ile Thr Glu Leu Ile Ser Gly Gln His Thr Thr Ala Thr Gln Thr 
385                 390                 395                 400 


Ile Leu Gly Arg Asn Ile Phe Arg Val Asp Ser Gln Ala Cys Asn Leu 
                405                 410                 415     


Asn Asp Thr Thr Tyr Gly Val Asn Arg Ala Val Phe Tyr His Asp Ala 
            420                 425                 430         


Ser Glu Gly Ser Gln Arg Ser Val Tyr Glu Gly Tyr Ile Arg Thr Thr 
        435                 440                 445             


Gly Ile Asp Asn Pro Arg Val Gln Asn Ile Asn Thr Tyr Leu Pro Gly 
    450                 455                 460                 


Glu Asn Ser Asp Ile Pro Thr Pro Glu Asp Tyr Thr His Ile Leu Ser 
465                 470                 475                 480 


Thr Thr Ile Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Ser Asn Arg 
                485                 490                 495     


Arg Ser Ser Leu Val Met Tyr Gly Trp Thr His Lys Ser Leu Ala Arg 
            500                 505                 510         


Asn Asn Thr Ile Asn Pro Asp Arg Ile Thr Gln Ile Pro Leu Thr Lys 
        515                 520                 525             


Val Asp Thr Arg Gly Thr Gly Val Ser Tyr Val Asn Asp Pro Gly Phe 
    530                 535                 540                 


Ile Gly Gly Ala Leu Leu Gln Arg Thr Asp His Gly Ser Leu Gly Val 
545                 550                 555                 560 


Leu Arg Val Gln Phe Pro Leu His Leu Arg Gln Gln Tyr Arg Ile Arg 
                565                 570                 575     


Val Arg Tyr Ala Ser Thr Thr Asn Ile Arg Leu Ser Val Asn Gly Ser 
            580                 585                 590         


Phe Gly Thr Ile Ser Gln Asn Leu Pro Ser Thr Met Arg Leu Gly Glu 
        595                 600                 605             


Asp Leu Arg Tyr Gly Ser Phe Ala Ile Arg Glu Phe Ser Thr Ser Ile 
    610                 615                 620                 


Arg Pro Thr Ala Ser Pro Asp Gln Ile Arg Leu Thr Ile Glu Pro Ser 
625                 630                 635                 640 


Phe Ile Arg Gln Glu Val Tyr Val Asp Arg Ile Glu Phe Ile Pro Val 
                645                 650                 655     


Asn Pro Thr Arg Glu Ala Lys Glu Asp Leu Glu Ala Thr Lys Lys Ala 
            660                 665                 670         


Val Ala Ser Leu Phe Thr Arg Thr Arg Asp Gly Leu Gln Val Asn Val 
        675                 680                 685             


Thr Asp Tyr Gln Val Asp Gln Ala Ala Asn Leu Val Ser Cys Leu Ser 
    690                 695                 700                 


Asp Glu Gln Tyr Ala His Asp Lys Lys Met Leu Leu Glu Ala Val Arg 
705                 710                 715                 720 


Ala Ala Lys Arg Leu Ser Arg Glu Arg Asn Leu Leu Gln Asp Pro Asp 
                725                 730                 735     


Phe Asn Thr Ile Asn Ser Thr Glu Glu Asn Gly Trp Lys Ala Ser Asn 
            740                 745                 750         


Gly Val Thr Ile Ser Glu Gly Gly Pro Phe Tyr Lys Gly Arg Ala Leu 
        755                 760                 765             


Gln Leu Ala Ser Ala Arg Glu Asn Tyr Pro Thr Tyr Ile Tyr Gln Lys 
    770                 775                 780                 


Val Asp Ala Ser Glu Leu Lys Pro Tyr Thr Arg Tyr Arg Leu Asp Gly 
785                 790                 795                 800 


Phe Val Lys Ser Ser Gln Asp Leu Glu Ile Asp Leu Ile His His His 
                805                 810                 815     


Lys Val His Leu Val Lys Asn Ala Pro Asp Asn Leu Val Ser Asp Thr 
            820                 825                 830         


Tyr Pro Asp Asp Ser Cys Ser Gly Ile Asn Arg Cys Gln Glu Gln Gln 
        835                 840                 845             


Met Val Asn Ala Gln Leu Glu Thr Glu His His His Pro Met Asp Cys 
    850                 855                 860                 


Cys Gly Ala Ala Gln Thr His Glu Phe Ser Ser Tyr Ile Asp Thr Gly 
865                 870                 875                 880 


Asp Leu Asn Ser Thr Val Asp Gln Gly Ile Trp Ala Ile Phe Lys Val 
                885                 890                 895     


Arg Thr Thr Asp Gly Tyr Ala Thr Leu Gly Asn Leu Glu Leu Val Glu 
            900                 905                 910         


Ile Gly Pro Leu Ser Gly Glu Ser Leu Glu Arg Glu Gln Arg Asp Asn 
        915                 920                 925             


Ala Lys Trp Ser Ala Glu Leu Gly Arg Lys Arg Ala Glu Thr Asp Arg 
    930                 935                 940                 


Val Tyr Gln Asp Ala Lys Gln Ser Ile Asn His Leu Phe Val Asp Tyr 
945                 950                 955                 960 


Gln Asp Gln Gln Leu Asn Pro Glu Ile Gly Met Ala Asp Ile Met Asp 
                965                 970                 975     


Ala Gln Asn Leu Val Ala Ser Ile Ser Asp Val Tyr Ser Asp Ala Val 
            980                 985                 990         


Leu Gln Ile Pro Gly Ile Asn Tyr  Glu Ile Tyr Thr Glu  Leu Ser Asn 
        995                 1000                 1005             


Arg Leu  Gln Gln Ala Ser Tyr  Leu Tyr Thr Ser Arg  Asn Ala Val 
    1010                 1015                 1020             


Gln Asn  Gly Asp Phe Asn Ser  Gly Leu Asp Ser Trp  Asn Ala Thr 
    1025                 1030                 1035             


Ala Gly  Ala Thr Val Gln Gln  Asp Gly Asn Thr His  Phe Leu Val 
    1040                 1045                 1050             


Leu Ser  His Trp Asp Ala Gln  Val Ser Gln Gln Phe  Arg Val Gln 
    1055                 1060                 1065             


Pro Asn  Cys Lys Tyr Val Leu  Arg Val Thr Ala Glu  Lys Val Gly 
    1070                 1075                 1080             


Gly Gly  Asp Gly Tyr Val Thr  Ile Arg Asp Gly Ala  His His Thr 
    1085                 1090                 1095             


Glu Thr  Leu Thr Phe Asn Ala  Cys Asp Tyr Asp Ile  Asn Gly Thr 
    1100                 1105                 1110             


Tyr Val  Thr Asp Asn Thr Tyr  Leu Thr Lys Glu Val  Val Phe Tyr 
    1115                 1120                 1125             


Ser His  Thr Glu His Met Trp  Val Glu Val Ser Glu  Thr Glu Gly 
    1130                 1135                 1140             


Val Phe  His Ile Asp Ser Val  Glu Phe Met Glu Thr  Gln Gln 
    1145                 1150                 1155         


<210>  23
<211>  1992
<212>  DNA
<213>  Artificial

<220>
<223>  Plant synthetic coding sequence encoding a TIC4064_15 pesticidal 
       protein with an additional alanine codon inserted immediately 
       following the initiating methionine and comprising a truncation 
       of the protoxin domain and mutations at S95T, R169K, and S332A.

<400>  23
atggctaacc agaacaagca cggcatcatt ggcgcatcca actgcgggtg taccagtgac       60

aacgtggcga aatacccgct cgccaataac ccatactcat cagccctgaa cctcaacagt      120

tgtcagaaca gctccatcct gaattggatc aacatcatcg gcgacgcggc gaaggaggcg      180

gtgagcatcg ggacaaccat cgtttcgctt atcacagctc cctcgctgac gggcctcatc      240

tcaatcgttt atgacctcat tgggaaggtg ctcggcggct cgaccggcca atccattagc      300

gacctttcca tctgcgatct gctctccata attgacctgc gggtgtcgca gtcggtcctg      360

aacgacggga tagcggactt caacggcagc gtcctcctgt accgcaacta cctggaggca      420

ctggactcct ggaacaagaa tcccaacagc gccagcgcgg aggagctgcg gacgcgcttt      480

cgcatcgctg attctgagtt tgacaagatc ctgacacgcg gctcccttac caacggcggt      540

agcctggctc gccagaacgc ccagattctg ttgctgccca gtttcgcgtc agccgcattc      600

tttcacctac ttctccttcg ggacgcaacg cgctacggga ccaattgggg attgtacaac      660

gcgacgccct tcatcaacta tcagtctaag ctcgtcgaac tgatcgaact ctacacggac      720

tactgcgtcc actggtacaa tcgtggcttt aacgagttgc gtcaacgcgg aacttctgct      780

acggcgtggc ttgagtttca cagataccgt cgtgagatga cgctaatggt cctcgacatc      840

gtggcgtcgt tcagctcgct tgacatcacc aactacccta tcgagaccga ctttcaactt      900

agccgtgtga tctacactga cccaatcggc ttcgtccatc gaagttcact ccgtggcgag      960

tcctggttct cgttcgtgaa ccgcgccaac ttcgctgacc ttgagaatgc gatccctaac     1020

ccacgcccta gctggttcct gaacaacatg attatctcta cagggagcct gaccttaccc     1080

gtcagtccca gcacagatcg cgcgagagtc tggtacggga gtcgggaccg gatctcgccc     1140

gccaactctc agttcattac tgagcttatc tccgggcagc acaccaccgc cacccagaca     1200

atactcggtc gcaacatctt ccgcgtggat tcacaggcgt gcaacctaaa cgacaccacg     1260

tacggcgtca accgcgccgt gttctaccat gacgcctcgg agggctcgca gcgtagcgtt     1320

tatgagggct acatcaggac gacgggtatc gacaacccga gagtgcagaa catcaacacc     1380

tatttgccag gcgagaacag cgacatcccg acgcccgaag actacaccca catcctcagc     1440

acaaccatca acctcacggg cgggctccgc caagttgcct cgaaccggcg gagcagcctc     1500

gttatgtacg gctggaccca caagtctctg gcgcgcaaca acaccatcaa tccagatcga     1560

atcacccaga taccactcac gaaggtggat acccgaggga ctggcgttag ttacgtcaac     1620

gatcctggat tcatcggcgg cgcgcttctc caacgcacgg accacgggtc gctgggcgtc     1680

cttcgggtac agtttccgct acacttgcgc cagcaatacc ggataagggt tcggtacgcc     1740

tcaacaacga acatcagact gtctgtcaac ggctccttcg gcaccatctc ccagaacctt     1800

ccctccacca tgcgcctcgg tgaggatctg cgctacggct ctttcgctat ccgcgagttt     1860

agcacttcaa tacgcccaac cgcgtcacct gaccagatac gcctgaccat tgaaccgtcc     1920

ttcatccggc aagaggtgta cgtggacaga atcgagttca taccagtcaa cccgacgcgg     1980

gaggcgaagt ga                                                         1992


<210>  24
<211>  663
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of TIC4064_15 with an additional alanine 
       amino acid inserted immediately following the initiating 
       methionine, and comprising a truncation at the protoxin domain 
       and mutations at S95T, R169K, and S332A.

<400>  24

Met Ala Asn Gln Asn Lys His Gly Ile Ile Gly Ala Ser Asn Cys Gly 
1               5                   10                  15      


Cys Thr Ser Asp Asn Val Ala Lys Tyr Pro Leu Ala Asn Asn Pro Tyr 
            20                  25                  30          


Ser Ser Ala Leu Asn Leu Asn Ser Cys Gln Asn Ser Ser Ile Leu Asn 
        35                  40                  45              


Trp Ile Asn Ile Ile Gly Asp Ala Ala Lys Glu Ala Val Ser Ile Gly 
    50                  55                  60                  


Thr Thr Ile Val Ser Leu Ile Thr Ala Pro Ser Leu Thr Gly Leu Ile 
65                  70                  75                  80  


Ser Ile Val Tyr Asp Leu Ile Gly Lys Val Leu Gly Gly Ser Thr Gly 
                85                  90                  95      


Gln Ser Ile Ser Asp Leu Ser Ile Cys Asp Leu Leu Ser Ile Ile Asp 
            100                 105                 110         


Leu Arg Val Ser Gln Ser Val Leu Asn Asp Gly Ile Ala Asp Phe Asn 
        115                 120                 125             


Gly Ser Val Leu Leu Tyr Arg Asn Tyr Leu Glu Ala Leu Asp Ser Trp 
    130                 135                 140                 


Asn Lys Asn Pro Asn Ser Ala Ser Ala Glu Glu Leu Arg Thr Arg Phe 
145                 150                 155                 160 


Arg Ile Ala Asp Ser Glu Phe Asp Lys Ile Leu Thr Arg Gly Ser Leu 
                165                 170                 175     


Thr Asn Gly Gly Ser Leu Ala Arg Gln Asn Ala Gln Ile Leu Leu Leu 
            180                 185                 190         


Pro Ser Phe Ala Ser Ala Ala Phe Phe His Leu Leu Leu Leu Arg Asp 
        195                 200                 205             


Ala Thr Arg Tyr Gly Thr Asn Trp Gly Leu Tyr Asn Ala Thr Pro Phe 
    210                 215                 220                 


Ile Asn Tyr Gln Ser Lys Leu Val Glu Leu Ile Glu Leu Tyr Thr Asp 
225                 230                 235                 240 


Tyr Cys Val His Trp Tyr Asn Arg Gly Phe Asn Glu Leu Arg Gln Arg 
                245                 250                 255     


Gly Thr Ser Ala Thr Ala Trp Leu Glu Phe His Arg Tyr Arg Arg Glu 
            260                 265                 270         


Met Thr Leu Met Val Leu Asp Ile Val Ala Ser Phe Ser Ser Leu Asp 
        275                 280                 285             


Ile Thr Asn Tyr Pro Ile Glu Thr Asp Phe Gln Leu Ser Arg Val Ile 
    290                 295                 300                 


Tyr Thr Asp Pro Ile Gly Phe Val His Arg Ser Ser Leu Arg Gly Glu 
305                 310                 315                 320 


Ser Trp Phe Ser Phe Val Asn Arg Ala Asn Phe Ala Asp Leu Glu Asn 
                325                 330                 335     


Ala Ile Pro Asn Pro Arg Pro Ser Trp Phe Leu Asn Asn Met Ile Ile 
            340                 345                 350         


Ser Thr Gly Ser Leu Thr Leu Pro Val Ser Pro Ser Thr Asp Arg Ala 
        355                 360                 365             


Arg Val Trp Tyr Gly Ser Arg Asp Arg Ile Ser Pro Ala Asn Ser Gln 
    370                 375                 380                 


Phe Ile Thr Glu Leu Ile Ser Gly Gln His Thr Thr Ala Thr Gln Thr 
385                 390                 395                 400 


Ile Leu Gly Arg Asn Ile Phe Arg Val Asp Ser Gln Ala Cys Asn Leu 
                405                 410                 415     


Asn Asp Thr Thr Tyr Gly Val Asn Arg Ala Val Phe Tyr His Asp Ala 
            420                 425                 430         


Ser Glu Gly Ser Gln Arg Ser Val Tyr Glu Gly Tyr Ile Arg Thr Thr 
        435                 440                 445             


Gly Ile Asp Asn Pro Arg Val Gln Asn Ile Asn Thr Tyr Leu Pro Gly 
    450                 455                 460                 


Glu Asn Ser Asp Ile Pro Thr Pro Glu Asp Tyr Thr His Ile Leu Ser 
465                 470                 475                 480 


Thr Thr Ile Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Ser Asn Arg 
                485                 490                 495     


Arg Ser Ser Leu Val Met Tyr Gly Trp Thr His Lys Ser Leu Ala Arg 
            500                 505                 510         


Asn Asn Thr Ile Asn Pro Asp Arg Ile Thr Gln Ile Pro Leu Thr Lys 
        515                 520                 525             


Val Asp Thr Arg Gly Thr Gly Val Ser Tyr Val Asn Asp Pro Gly Phe 
    530                 535                 540                 


Ile Gly Gly Ala Leu Leu Gln Arg Thr Asp His Gly Ser Leu Gly Val 
545                 550                 555                 560 


Leu Arg Val Gln Phe Pro Leu His Leu Arg Gln Gln Tyr Arg Ile Arg 
                565                 570                 575     


Val Arg Tyr Ala Ser Thr Thr Asn Ile Arg Leu Ser Val Asn Gly Ser 
            580                 585                 590         


Phe Gly Thr Ile Ser Gln Asn Leu Pro Ser Thr Met Arg Leu Gly Glu 
        595                 600                 605             


Asp Leu Arg Tyr Gly Ser Phe Ala Ile Arg Glu Phe Ser Thr Ser Ile 
    610                 615                 620                 


Arg Pro Thr Ala Ser Pro Asp Gln Ile Arg Leu Thr Ile Glu Pro Ser 
625                 630                 635                 640 


Phe Ile Arg Gln Glu Val Tyr Val Asp Arg Ile Glu Phe Ile Pro Val 
                645                 650                 655     


Asn Pro Thr Arg Glu Ala Lys 
            660             


<210>  25
<211>  3474
<212>  DNA
<213>  Artificial

<220>
<223>  Plant synthetic coding sequence encoding a TIC4064_16 pesticidal 
       protein with an additional alanine codon inserted immediately 
       following the initiating methionine and comprising mutations at 
       S34G, G88K, I386S, G403Q, and R605N.

<400>  25
atggctaacc agaacaagca cggcatcatt ggcgcatcca actgcgggtg taccagtgac       60

aacgtggcga aatacccgct cgccaataac ccatactcag gagccctgaa cctcaacagt      120

tgtcagaaca gctccatcct gaattggatc aacatcatcg gcgacgcggc gaaggaggcg      180

gtgagcatcg ggacaaccat cgtttcgctt atcacagctc cctcgctgac gggcctcatc      240

tcaatcgttt atgacctcat taagaaggtg ctcggcggct cgtccggcca atccattagc      300

gacctttcca tctgcgatct gctctccata attgacctgc gggtgtcgca gtcggtcctg      360

aacgacggga tagcggactt caacggcagc gtcctcctgt accgcaacta cctggaggca      420

ctggactcct ggaacaagaa tcccaacagc gccagcgcgg aggagctgcg gacgcgcttt      480

cgcatcgctg attctgagtt tgaccgaatc ctgacacgcg gctcccttac caacggcggt      540

agcctggctc gccagaacgc ccagattctg ttgctgccca gtttcgcgtc agccgcattc      600

tttcacctac ttctccttcg ggacgcaacg cgctacggga ccaattgggg attgtacaac      660

gcgacgccct tcatcaacta tcagtctaag ctcgtcgaac tgatcgaact ctacacggac      720

tactgcgtcc actggtacaa tcgtggcttt aacgagttgc gtcaacgcgg aacttctgct      780

acggcgtggc ttgagtttca cagataccgt cgtgagatga cgctaatggt cctcgacatc      840

gtggcgtcgt tcagctcgct tgacatcacc aactacccta tcgagaccga ctttcaactt      900

agccgtgtga tctacactga cccaatcggc ttcgtccatc gaagttcact ccgtggcgag      960

tcctggttct cgttcgtgaa ccgcgccaac ttcagtgacc ttgagaatgc gatccctaac     1020

ccacgcccta gctggttcct gaacaacatg attatctcta cagggagcct gaccttaccc     1080

gtcagtccca gcacagatcg cgcgagagtc tggtacggga gtcgggaccg gatctcgccc     1140

gccaactctc agttcagtac tgagcttatc tccgggcagc acaccaccgc cacccagaca     1200

atactccagc gcaacatctt ccgcgtggat tcacaggcgt gcaacctaaa cgacaccacg     1260

tacggcgtca accgcgccgt gttctaccat gacgcctcgg agggctcgca gcgtagcgtt     1320

tatgagggct acatcaggac gacgggtatc gacaacccga gagtgcagaa catcaacacc     1380

tatttgccag gcgagaacag cgacatcccg acgcccgaag actacaccca catcctcagc     1440

acaaccatca acctcacggg cgggctccgc caagttgcct cgaaccggcg gagcagcctc     1500

gttatgtacg gctggaccca caagtctctg gcgcgcaaca acaccatcaa tccagatcga     1560

atcacccaga taccactcac gaaggtggat acccgaggga ctggcgttag ttacgtcaac     1620

gatcctggat tcatcggcgg cgcgcttctc caacgcacgg accacgggtc gctgggcgtc     1680

cttcgggtac agtttccgct acacttgcgc cagcaatacc ggataagggt tcggtacgcc     1740

tcaacaacga acatcagact gtctgtcaac ggctccttcg gcaccatctc ccagaacctt     1800

ccctccacca tgaacctcgg tgaggatctg cgctacggct ctttcgctat ccgcgagttt     1860

agcacttcaa tacgcccaac cgcgtcacct gaccagatac gcctgaccat tgaaccgtcc     1920

ttcatccggc aagaggtgta cgtggacaga atcgagttca taccagtcaa cccgacgcgg     1980

gaggcgaagg aagaccttga ggctaccaag aaggcggtgg cgagcctgtt tacccgcact     2040

cgtgacggcc tccaagtaaa tgtcacggac taccaagtgg accaggcggc caacctcgta     2100

agctgcctat cagacgagca gtacgcccac gacaagaaga tgcttctgga agccgtcaga     2160

gccgccaaac ggctatcgcg cgagcgcaac ctgctccaag atcccgactt caacactatc     2220

aacagcaccg aggagaatgg ctggaaagcc agcaacggcg tgacgattag cgagggcggc     2280

ccgttctaca agggccgcgc actacagctt gcctccgcaa gggagaacta ccctacctac     2340

atctaccaga aggtcgatgc gtcggaactt aaaccataca cacggtatcg tctcgacggg     2400

ttcgtcaaat ccagccaaga cctagagatc gacctcatcc accatcacaa agtacatctc     2460

gtcaagaatg cgcccgacaa tcttgtctct gacacttacc cggacgattc ctgttcgggt     2520

attaacagat gccaggagca gcagatggtg aacgcccaac tcgaaaccga gcaccatcac     2580

ccaatggact gctgcggtgc ggctcagacc cacgagttta gcagttacat cgacaccggc     2640

gacctgaact cgacggtgga ccagggcatc tgggcgatct tcaaggttcg taccaccgac     2700

ggctacgcga ctctcgggaa tctggaactg gtcgagatcg gcccgttgag cggcgagtcg     2760

ctggagcggg agcagaggga caacgcaaag tggtctgccg agctgggccg aaagcgagcg     2820

gagactgacc gcgtgtacca ggatgctaag caaagtatca accacctctt cgtggactat     2880

caagatcaac agctcaaccc tgagatcggg atggctgaca tcatggacgc gcagaacctg     2940

gtggcgtcga tctccgatgt gtactccgac gccgtgctcc aaatccctgg cattaactac     3000

gagatctaca cggagctgtc gaaccgcctc caacaagcga gctaccttta cacgagccgg     3060

aacgccgtac agaacggcga cttcaactcc gggctcgact catggaacgc cactgcgggc     3120

gctaccgtcc aacaagacgg caacacccac tttctcgtcc ttagccactg ggacgcccaa     3180

gtctcgcagc agtttcgcgt tcagccgaac tgcaagtatg tcctgcgcgt gaccgccgag     3240

aaggtgggcg gtggtgacgg ctacgtcacc ataagggatg gtgcccacca cacggagacc     3300

ttaacgttta acgcctgtga ttatgacatc aacggcacct atgtgacgga caatacctac     3360

ctcactaagg aagtcgtgtt ctattcccat accgagcaca tgtgggtcga ggtttcggag     3420

acggagggcg tattccacat cgacagtgtg gagttcatgg agacccagca gtga           3474


<210>  26
<211>  1157
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of TIC4064_16 with an additional alanine 
       amino acid inserted immediately following the initiating 
       methionine and comprising mutations at S34G, G88K, I386S, G403Q, 
       and R605N.

<400>  26

Met Ala Asn Gln Asn Lys His Gly Ile Ile Gly Ala Ser Asn Cys Gly 
1               5                   10                  15      


Cys Thr Ser Asp Asn Val Ala Lys Tyr Pro Leu Ala Asn Asn Pro Tyr 
            20                  25                  30          


Ser Gly Ala Leu Asn Leu Asn Ser Cys Gln Asn Ser Ser Ile Leu Asn 
        35                  40                  45              


Trp Ile Asn Ile Ile Gly Asp Ala Ala Lys Glu Ala Val Ser Ile Gly 
    50                  55                  60                  


Thr Thr Ile Val Ser Leu Ile Thr Ala Pro Ser Leu Thr Gly Leu Ile 
65                  70                  75                  80  


Ser Ile Val Tyr Asp Leu Ile Lys Lys Val Leu Gly Gly Ser Ser Gly 
                85                  90                  95      


Gln Ser Ile Ser Asp Leu Ser Ile Cys Asp Leu Leu Ser Ile Ile Asp 
            100                 105                 110         


Leu Arg Val Ser Gln Ser Val Leu Asn Asp Gly Ile Ala Asp Phe Asn 
        115                 120                 125             


Gly Ser Val Leu Leu Tyr Arg Asn Tyr Leu Glu Ala Leu Asp Ser Trp 
    130                 135                 140                 


Asn Lys Asn Pro Asn Ser Ala Ser Ala Glu Glu Leu Arg Thr Arg Phe 
145                 150                 155                 160 


Arg Ile Ala Asp Ser Glu Phe Asp Arg Ile Leu Thr Arg Gly Ser Leu 
                165                 170                 175     


Thr Asn Gly Gly Ser Leu Ala Arg Gln Asn Ala Gln Ile Leu Leu Leu 
            180                 185                 190         


Pro Ser Phe Ala Ser Ala Ala Phe Phe His Leu Leu Leu Leu Arg Asp 
        195                 200                 205             


Ala Thr Arg Tyr Gly Thr Asn Trp Gly Leu Tyr Asn Ala Thr Pro Phe 
    210                 215                 220                 


Ile Asn Tyr Gln Ser Lys Leu Val Glu Leu Ile Glu Leu Tyr Thr Asp 
225                 230                 235                 240 


Tyr Cys Val His Trp Tyr Asn Arg Gly Phe Asn Glu Leu Arg Gln Arg 
                245                 250                 255     


Gly Thr Ser Ala Thr Ala Trp Leu Glu Phe His Arg Tyr Arg Arg Glu 
            260                 265                 270         


Met Thr Leu Met Val Leu Asp Ile Val Ala Ser Phe Ser Ser Leu Asp 
        275                 280                 285             


Ile Thr Asn Tyr Pro Ile Glu Thr Asp Phe Gln Leu Ser Arg Val Ile 
    290                 295                 300                 


Tyr Thr Asp Pro Ile Gly Phe Val His Arg Ser Ser Leu Arg Gly Glu 
305                 310                 315                 320 


Ser Trp Phe Ser Phe Val Asn Arg Ala Asn Phe Ser Asp Leu Glu Asn 
                325                 330                 335     


Ala Ile Pro Asn Pro Arg Pro Ser Trp Phe Leu Asn Asn Met Ile Ile 
            340                 345                 350         


Ser Thr Gly Ser Leu Thr Leu Pro Val Ser Pro Ser Thr Asp Arg Ala 
        355                 360                 365             


Arg Val Trp Tyr Gly Ser Arg Asp Arg Ile Ser Pro Ala Asn Ser Gln 
    370                 375                 380                 


Phe Ser Thr Glu Leu Ile Ser Gly Gln His Thr Thr Ala Thr Gln Thr 
385                 390                 395                 400 


Ile Leu Gln Arg Asn Ile Phe Arg Val Asp Ser Gln Ala Cys Asn Leu 
                405                 410                 415     


Asn Asp Thr Thr Tyr Gly Val Asn Arg Ala Val Phe Tyr His Asp Ala 
            420                 425                 430         


Ser Glu Gly Ser Gln Arg Ser Val Tyr Glu Gly Tyr Ile Arg Thr Thr 
        435                 440                 445             


Gly Ile Asp Asn Pro Arg Val Gln Asn Ile Asn Thr Tyr Leu Pro Gly 
    450                 455                 460                 


Glu Asn Ser Asp Ile Pro Thr Pro Glu Asp Tyr Thr His Ile Leu Ser 
465                 470                 475                 480 


Thr Thr Ile Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Ser Asn Arg 
                485                 490                 495     


Arg Ser Ser Leu Val Met Tyr Gly Trp Thr His Lys Ser Leu Ala Arg 
            500                 505                 510         


Asn Asn Thr Ile Asn Pro Asp Arg Ile Thr Gln Ile Pro Leu Thr Lys 
        515                 520                 525             


Val Asp Thr Arg Gly Thr Gly Val Ser Tyr Val Asn Asp Pro Gly Phe 
    530                 535                 540                 


Ile Gly Gly Ala Leu Leu Gln Arg Thr Asp His Gly Ser Leu Gly Val 
545                 550                 555                 560 


Leu Arg Val Gln Phe Pro Leu His Leu Arg Gln Gln Tyr Arg Ile Arg 
                565                 570                 575     


Val Arg Tyr Ala Ser Thr Thr Asn Ile Arg Leu Ser Val Asn Gly Ser 
            580                 585                 590         


Phe Gly Thr Ile Ser Gln Asn Leu Pro Ser Thr Met Asn Leu Gly Glu 
        595                 600                 605             


Asp Leu Arg Tyr Gly Ser Phe Ala Ile Arg Glu Phe Ser Thr Ser Ile 
    610                 615                 620                 


Arg Pro Thr Ala Ser Pro Asp Gln Ile Arg Leu Thr Ile Glu Pro Ser 
625                 630                 635                 640 


Phe Ile Arg Gln Glu Val Tyr Val Asp Arg Ile Glu Phe Ile Pro Val 
                645                 650                 655     


Asn Pro Thr Arg Glu Ala Lys Glu Asp Leu Glu Ala Thr Lys Lys Ala 
            660                 665                 670         


Val Ala Ser Leu Phe Thr Arg Thr Arg Asp Gly Leu Gln Val Asn Val 
        675                 680                 685             


Thr Asp Tyr Gln Val Asp Gln Ala Ala Asn Leu Val Ser Cys Leu Ser 
    690                 695                 700                 


Asp Glu Gln Tyr Ala His Asp Lys Lys Met Leu Leu Glu Ala Val Arg 
705                 710                 715                 720 


Ala Ala Lys Arg Leu Ser Arg Glu Arg Asn Leu Leu Gln Asp Pro Asp 
                725                 730                 735     


Phe Asn Thr Ile Asn Ser Thr Glu Glu Asn Gly Trp Lys Ala Ser Asn 
            740                 745                 750         


Gly Val Thr Ile Ser Glu Gly Gly Pro Phe Tyr Lys Gly Arg Ala Leu 
        755                 760                 765             


Gln Leu Ala Ser Ala Arg Glu Asn Tyr Pro Thr Tyr Ile Tyr Gln Lys 
    770                 775                 780                 


Val Asp Ala Ser Glu Leu Lys Pro Tyr Thr Arg Tyr Arg Leu Asp Gly 
785                 790                 795                 800 


Phe Val Lys Ser Ser Gln Asp Leu Glu Ile Asp Leu Ile His His His 
                805                 810                 815     


Lys Val His Leu Val Lys Asn Ala Pro Asp Asn Leu Val Ser Asp Thr 
            820                 825                 830         


Tyr Pro Asp Asp Ser Cys Ser Gly Ile Asn Arg Cys Gln Glu Gln Gln 
        835                 840                 845             


Met Val Asn Ala Gln Leu Glu Thr Glu His His His Pro Met Asp Cys 
    850                 855                 860                 


Cys Gly Ala Ala Gln Thr His Glu Phe Ser Ser Tyr Ile Asp Thr Gly 
865                 870                 875                 880 


Asp Leu Asn Ser Thr Val Asp Gln Gly Ile Trp Ala Ile Phe Lys Val 
                885                 890                 895     


Arg Thr Thr Asp Gly Tyr Ala Thr Leu Gly Asn Leu Glu Leu Val Glu 
            900                 905                 910         


Ile Gly Pro Leu Ser Gly Glu Ser Leu Glu Arg Glu Gln Arg Asp Asn 
        915                 920                 925             


Ala Lys Trp Ser Ala Glu Leu Gly Arg Lys Arg Ala Glu Thr Asp Arg 
    930                 935                 940                 


Val Tyr Gln Asp Ala Lys Gln Ser Ile Asn His Leu Phe Val Asp Tyr 
945                 950                 955                 960 


Gln Asp Gln Gln Leu Asn Pro Glu Ile Gly Met Ala Asp Ile Met Asp 
                965                 970                 975     


Ala Gln Asn Leu Val Ala Ser Ile Ser Asp Val Tyr Ser Asp Ala Val 
            980                 985                 990         


Leu Gln Ile Pro Gly Ile Asn Tyr  Glu Ile Tyr Thr Glu  Leu Ser Asn 
        995                 1000                 1005             


Arg Leu  Gln Gln Ala Ser Tyr  Leu Tyr Thr Ser Arg  Asn Ala Val 
    1010                 1015                 1020             


Gln Asn  Gly Asp Phe Asn Ser  Gly Leu Asp Ser Trp  Asn Ala Thr 
    1025                 1030                 1035             


Ala Gly  Ala Thr Val Gln Gln  Asp Gly Asn Thr His  Phe Leu Val 
    1040                 1045                 1050             


Leu Ser  His Trp Asp Ala Gln  Val Ser Gln Gln Phe  Arg Val Gln 
    1055                 1060                 1065             


Pro Asn  Cys Lys Tyr Val Leu  Arg Val Thr Ala Glu  Lys Val Gly 
    1070                 1075                 1080             


Gly Gly  Asp Gly Tyr Val Thr  Ile Arg Asp Gly Ala  His His Thr 
    1085                 1090                 1095             


Glu Thr  Leu Thr Phe Asn Ala  Cys Asp Tyr Asp Ile  Asn Gly Thr 
    1100                 1105                 1110             


Tyr Val  Thr Asp Asn Thr Tyr  Leu Thr Lys Glu Val  Val Phe Tyr 
    1115                 1120                 1125             


Ser His  Thr Glu His Met Trp  Val Glu Val Ser Glu  Thr Glu Gly 
    1130                 1135                 1140             


Val Phe  His Ile Asp Ser Val  Glu Phe Met Glu Thr  Gln Gln 
    1145                 1150                 1155         


<210>  27
<211>  1992
<212>  DNA
<213>  Artificial

<220>
<223>  Plant synthetic coding sequence encoding a TIC4064_17 pesticidal 
       protein with an additional alanine codon inserted immediately 
       following the initiating methionine and comprising a protoxin 
       domain truncation and mutations at S34G, G88K, I386S, G403Q, and 
       

<400>  27
atggctaacc agaacaagca cggcatcatt ggcgcatcca actgcgggtg taccagtgac       60

aacgtggcga aatacccgct cgccaataac ccatactcag gagccctgaa cctcaacagt      120

tgtcagaaca gctccatcct gaattggatc aacatcatcg gcgacgcggc gaaggaggcg      180

gtgagcatcg ggacaaccat cgtttcgctt atcacagctc cctcgctgac gggcctcatc      240

tcaatcgttt atgacctcat taagaaggtg ctcggcggct cgtccggcca atccattagc      300

gacctttcca tctgcgatct gctctccata attgacctgc gggtgtcgca gtcggtcctg      360

aacgacggga tagcggactt caacggcagc gtcctcctgt accgcaacta cctggaggca      420

ctggactcct ggaacaagaa tcccaacagc gccagcgcgg aggagctgcg gacgcgcttt      480

cgcatcgctg attctgagtt tgaccgaatc ctgacacgcg gctcccttac caacggcggt      540

agcctggctc gccagaacgc ccagattctg ttgctgccca gtttcgcgtc agccgcattc      600

tttcacctac ttctccttcg ggacgcaacg cgctacggga ccaattgggg attgtacaac      660

gcgacgccct tcatcaacta tcagtctaag ctcgtcgaac tgatcgaact ctacacggac      720

tactgcgtcc actggtacaa tcgtggcttt aacgagttgc gtcaacgcgg aacttctgct      780

acggcgtggc ttgagtttca cagataccgt cgtgagatga cgctaatggt cctcgacatc      840

gtggcgtcgt tcagctcgct tgacatcacc aactacccta tcgagaccga ctttcaactt      900

agccgtgtga tctacactga cccaatcggc ttcgtccatc gaagttcact ccgtggcgag      960

tcctggttct cgttcgtgaa ccgcgccaac ttcagtgacc ttgagaatgc gatccctaac     1020

ccacgcccta gctggttcct gaacaacatg attatctcta cagggagcct gaccttaccc     1080

gtcagtccca gcacagatcg cgcgagagtc tggtacggga gtcgggaccg gatctcgccc     1140

gccaactctc agttcagtac tgagcttatc tccgggcagc acaccaccgc cacccagaca     1200

atactccagc gcaacatctt ccgcgtggat tcacaggcgt gcaacctaaa cgacaccacg     1260

tacggcgtca accgcgccgt gttctaccat gacgcctcgg agggctcgca gcgtagcgtt     1320

tatgagggct acatcaggac gacgggtatc gacaacccga gagtgcagaa catcaacacc     1380

tatttgccag gcgagaacag cgacatcccg acgcccgaag actacaccca catcctcagc     1440

acaaccatca acctcacggg cgggctccgc caagttgcct cgaaccggcg gagcagcctc     1500

gttatgtacg gctggaccca caagtctctg gcgcgcaaca acaccatcaa tccagatcga     1560

atcacccaga taccactcac gaaggtggat acccgaggga ctggcgttag ttacgtcaac     1620

gatcctggat tcatcggcgg cgcgcttctc caacgcacgg accacgggtc gctgggcgtc     1680

cttcgggtac agtttccgct acacttgcgc cagcaatacc ggataagggt tcggtacgcc     1740

tcaacaacga acatcagact gtctgtcaac ggctccttcg gcaccatctc ccagaacctt     1800

ccctccacca tgaacctcgg tgaggatctg cgctacggct ctttcgctat ccgcgagttt     1860

agcacttcaa tacgcccaac cgcgtcacct gaccagatac gcctgaccat tgaaccgtcc     1920

ttcatccggc aagaggtgta cgtggacaga atcgagttca taccagtcaa cccgacgcgg     1980

gaggcgaagt ga                                                         1992


<210>  28
<211>  663
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of TIC4064_15 with an additional alanine 
       amino acid inserted immediately following the initiating 
       methionine and comprising a protoxin domain truncation and 
       mutations at S34G, G88K, I386S, G403Q, and R605N.

<400>  28

Met Ala Asn Gln Asn Lys His Gly Ile Ile Gly Ala Ser Asn Cys Gly 
1               5                   10                  15      


Cys Thr Ser Asp Asn Val Ala Lys Tyr Pro Leu Ala Asn Asn Pro Tyr 
            20                  25                  30          


Ser Gly Ala Leu Asn Leu Asn Ser Cys Gln Asn Ser Ser Ile Leu Asn 
        35                  40                  45              


Trp Ile Asn Ile Ile Gly Asp Ala Ala Lys Glu Ala Val Ser Ile Gly 
    50                  55                  60                  


Thr Thr Ile Val Ser Leu Ile Thr Ala Pro Ser Leu Thr Gly Leu Ile 
65                  70                  75                  80  


Ser Ile Val Tyr Asp Leu Ile Lys Lys Val Leu Gly Gly Ser Ser Gly 
                85                  90                  95      


Gln Ser Ile Ser Asp Leu Ser Ile Cys Asp Leu Leu Ser Ile Ile Asp 
            100                 105                 110         


Leu Arg Val Ser Gln Ser Val Leu Asn Asp Gly Ile Ala Asp Phe Asn 
        115                 120                 125             


Gly Ser Val Leu Leu Tyr Arg Asn Tyr Leu Glu Ala Leu Asp Ser Trp 
    130                 135                 140                 


Asn Lys Asn Pro Asn Ser Ala Ser Ala Glu Glu Leu Arg Thr Arg Phe 
145                 150                 155                 160 


Arg Ile Ala Asp Ser Glu Phe Asp Arg Ile Leu Thr Arg Gly Ser Leu 
                165                 170                 175     


Thr Asn Gly Gly Ser Leu Ala Arg Gln Asn Ala Gln Ile Leu Leu Leu 
            180                 185                 190         


Pro Ser Phe Ala Ser Ala Ala Phe Phe His Leu Leu Leu Leu Arg Asp 
        195                 200                 205             


Ala Thr Arg Tyr Gly Thr Asn Trp Gly Leu Tyr Asn Ala Thr Pro Phe 
    210                 215                 220                 


Ile Asn Tyr Gln Ser Lys Leu Val Glu Leu Ile Glu Leu Tyr Thr Asp 
225                 230                 235                 240 


Tyr Cys Val His Trp Tyr Asn Arg Gly Phe Asn Glu Leu Arg Gln Arg 
                245                 250                 255     


Gly Thr Ser Ala Thr Ala Trp Leu Glu Phe His Arg Tyr Arg Arg Glu 
            260                 265                 270         


Met Thr Leu Met Val Leu Asp Ile Val Ala Ser Phe Ser Ser Leu Asp 
        275                 280                 285             


Ile Thr Asn Tyr Pro Ile Glu Thr Asp Phe Gln Leu Ser Arg Val Ile 
    290                 295                 300                 


Tyr Thr Asp Pro Ile Gly Phe Val His Arg Ser Ser Leu Arg Gly Glu 
305                 310                 315                 320 


Ser Trp Phe Ser Phe Val Asn Arg Ala Asn Phe Ser Asp Leu Glu Asn 
                325                 330                 335     


Ala Ile Pro Asn Pro Arg Pro Ser Trp Phe Leu Asn Asn Met Ile Ile 
            340                 345                 350         


Ser Thr Gly Ser Leu Thr Leu Pro Val Ser Pro Ser Thr Asp Arg Ala 
        355                 360                 365             


Arg Val Trp Tyr Gly Ser Arg Asp Arg Ile Ser Pro Ala Asn Ser Gln 
    370                 375                 380                 


Phe Ser Thr Glu Leu Ile Ser Gly Gln His Thr Thr Ala Thr Gln Thr 
385                 390                 395                 400 


Ile Leu Gln Arg Asn Ile Phe Arg Val Asp Ser Gln Ala Cys Asn Leu 
                405                 410                 415     


Asn Asp Thr Thr Tyr Gly Val Asn Arg Ala Val Phe Tyr His Asp Ala 
            420                 425                 430         


Ser Glu Gly Ser Gln Arg Ser Val Tyr Glu Gly Tyr Ile Arg Thr Thr 
        435                 440                 445             


Gly Ile Asp Asn Pro Arg Val Gln Asn Ile Asn Thr Tyr Leu Pro Gly 
    450                 455                 460                 


Glu Asn Ser Asp Ile Pro Thr Pro Glu Asp Tyr Thr His Ile Leu Ser 
465                 470                 475                 480 


Thr Thr Ile Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Ser Asn Arg 
                485                 490                 495     


Arg Ser Ser Leu Val Met Tyr Gly Trp Thr His Lys Ser Leu Ala Arg 
            500                 505                 510         


Asn Asn Thr Ile Asn Pro Asp Arg Ile Thr Gln Ile Pro Leu Thr Lys 
        515                 520                 525             


Val Asp Thr Arg Gly Thr Gly Val Ser Tyr Val Asn Asp Pro Gly Phe 
    530                 535                 540                 


Ile Gly Gly Ala Leu Leu Gln Arg Thr Asp His Gly Ser Leu Gly Val 
545                 550                 555                 560 


Leu Arg Val Gln Phe Pro Leu His Leu Arg Gln Gln Tyr Arg Ile Arg 
                565                 570                 575     


Val Arg Tyr Ala Ser Thr Thr Asn Ile Arg Leu Ser Val Asn Gly Ser 
            580                 585                 590         


Phe Gly Thr Ile Ser Gln Asn Leu Pro Ser Thr Met Asn Leu Gly Glu 
        595                 600                 605             


Asp Leu Arg Tyr Gly Ser Phe Ala Ile Arg Glu Phe Ser Thr Ser Ile 
    610                 615                 620                 


Arg Pro Thr Ala Ser Pro Asp Gln Ile Arg Leu Thr Ile Glu Pro Ser 
625                 630                 635                 640 


Phe Ile Arg Gln Glu Val Tyr Val Asp Arg Ile Glu Phe Ile Pro Val 
                645                 650                 655     


Asn Pro Thr Arg Glu Ala Lys 
            660             


<210>  29
<211>  3474
<212>  DNA
<213>  Artificial

<220>
<223>  Plant synthetic coding sequence encoding a TIC4064_18 pesticidal 
       protein with an additional alanine codon inserted immediately 
       following the initiating methionine and comprising mutations at 
       G88K, W371L, H555N, and R586Q.

<400>  29
atggctaacc agaacaagca cggcatcatt ggcgcatcca actgcgggtg taccagtgac       60

aacgtggcga aatacccgct cgccaataac ccatactcat cagccctgaa cctcaacagt      120

tgtcagaaca gctccatcct gaattggatc aacatcatcg gcgacgcggc gaaggaggcg      180

gtgagcatcg ggacaaccat cgtttcgctt atcacagctc cctcgctgac gggcctcatc      240

tcaatcgttt atgacctcat taagaaggtg ctcggcggct cgtccggcca atccattagc      300

gacctttcca tctgcgatct gctctccata attgacctgc gggtgtcgca gtcggtcctg      360

aacgacggga tagcggactt caacggcagc gtcctcctgt accgcaacta cctggaggca      420

ctggactcct ggaacaagaa tcccaacagc gccagcgcgg aggagctgcg gacgcgcttt      480

cgcatcgctg attctgagtt tgaccgaatc ctgacacgcg gctcccttac caacggcggt      540

agcctggctc gccagaacgc ccagattctg ttgctgccca gtttcgcgtc agccgcattc      600

tttcacctac ttctccttcg ggacgcaacg cgctacggga ccaattgggg attgtacaac      660

gcgacgccct tcatcaacta tcagtctaag ctcgtcgaac tgatcgaact ctacacggac      720

tactgcgtcc actggtacaa tcgtggcttt aacgagttgc gtcaacgcgg aacttctgct      780

acggcgtggc ttgagtttca cagataccgt cgtgagatga cgctaatggt cctcgacatc      840

gtggcgtcgt tcagctcgct tgacatcacc aactacccta tcgagaccga ctttcaactt      900

agccgtgtga tctacactga cccaatcggc ttcgtccatc gaagttcact ccgtggcgag      960

tcctggttct cgttcgtgaa ccgcgccaac ttcagtgacc ttgagaatgc gatccctaac     1020

ccacgcccta gctggttcct gaacaacatg attatctcta cagggagcct gaccttaccc     1080

gtcagtccca gcacagatcg cgcgagagtc ttgtacggga gtcgggaccg gatctcgccc     1140

gccaactctc agttcattac tgagcttatc tccgggcagc acaccaccgc cacccagaca     1200

atactcggtc gcaacatctt ccgcgtggat tcacaggcgt gcaacctaaa cgacaccacg     1260

tacggcgtca accgcgccgt gttctaccat gacgcctcgg agggctcgca gcgtagcgtt     1320

tatgagggct acatcaggac gacgggtatc gacaacccga gagtgcagaa catcaacacc     1380

tatttgccag gcgagaacag cgacatcccg acgcccgaag actacaccca catcctcagc     1440

acaaccatca acctcacggg cgggctccgc caagttgcct cgaaccggcg gagcagcctc     1500

gttatgtacg gctggaccca caagtctctg gcgcgcaaca acaccatcaa tccagatcga     1560

atcacccaga taccactcac gaaggtggat acccgaggga ctggcgttag ttacgtcaac     1620

gatcctggat tcatcggcgg cgcgcttctc caacgcacgg acaacgggtc gctgggcgtc     1680

cttcgggtac agtttccgct acacttgcgc cagcaatacc ggataagggt tcggtacgcc     1740

tcaacaacga acatccaact gtctgtcaac ggctccttcg gcaccatctc ccagaacctt     1800

ccctccacca tgcgcctcgg tgaggatctg cgctacggct ctttcgctat ccgcgagttt     1860

agcacttcaa tacgcccaac cgcgtcacct gaccagatac gcctgaccat tgaaccgtcc     1920

ttcatccggc aagaggtgta cgtggacaga atcgagttca taccagtcaa cccgacgcgg     1980

gaggcgaagg aagaccttga ggctaccaag aaggcggtgg cgagcctgtt tacccgcact     2040

cgtgacggcc tccaagtaaa tgtcacggac taccaagtgg accaggcggc caacctcgta     2100

agctgcctat cagacgagca gtacgcccac gacaagaaga tgcttctgga agccgtcaga     2160

gccgccaaac ggctatcgcg cgagcgcaac ctgctccaag atcccgactt caacactatc     2220

aacagcaccg aggagaatgg ctggaaagcc agcaacggcg tgacgattag cgagggcggc     2280

ccgttctaca agggccgcgc actacagctt gcctccgcaa gggagaacta ccctacctac     2340

atctaccaga aggtcgatgc gtcggaactt aaaccataca cacggtatcg tctcgacggg     2400

ttcgtcaaat ccagccaaga cctagagatc gacctcatcc accatcacaa agtacatctc     2460

gtcaagaatg cgcccgacaa tcttgtctct gacacttacc cggacgattc ctgttcgggt     2520

attaacagat gccaggagca gcagatggtg aacgcccaac tcgaaaccga gcaccatcac     2580

ccaatggact gctgcggtgc ggctcagacc cacgagttta gcagttacat cgacaccggc     2640

gacctgaact cgacggtgga ccagggcatc tgggcgatct tcaaggttcg taccaccgac     2700

ggctacgcga ctctcgggaa tctggaactg gtcgagatcg gcccgttgag cggcgagtcg     2760

ctggagcggg agcagaggga caacgcaaag tggtctgccg agctgggccg aaagcgagcg     2820

gagactgacc gcgtgtacca ggatgctaag caaagtatca accacctctt cgtggactat     2880

caagatcaac agctcaaccc tgagatcggg atggctgaca tcatggacgc gcagaacctg     2940

gtggcgtcga tctccgatgt gtactccgac gccgtgctcc aaatccctgg cattaactac     3000

gagatctaca cggagctgtc gaaccgcctc caacaagcga gctaccttta cacgagccgg     3060

aacgccgtac agaacggcga cttcaactcc gggctcgact catggaacgc cactgcgggc     3120

gctaccgtcc aacaagacgg caacacccac tttctcgtcc ttagccactg ggacgcccaa     3180

gtctcgcagc agtttcgcgt tcagccgaac tgcaagtatg tcctgcgcgt gaccgccgag     3240

aaggtgggcg gtggtgacgg ctacgtcacc ataagggatg gtgcccacca cacggagacc     3300

ttaacgttta acgcctgtga ttatgacatc aacggcacct atgtgacgga caatacctac     3360

ctcactaagg aagtcgtgtt ctattcccat accgagcaca tgtgggtcga ggtttcggag     3420

acggagggcg tattccacat cgacagtgtg gagttcatgg agacccagca gtga           3474


<210>  30
<211>  1157
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of TIC4064_18 with an additional alanine 
       amino acid inserted immediately following the initiating 
       methionine and comprising mutations at G88K, W371L, H555N, and 
       R586Q.

<400>  30

Met Ala Asn Gln Asn Lys His Gly Ile Ile Gly Ala Ser Asn Cys Gly 
1               5                   10                  15      


Cys Thr Ser Asp Asn Val Ala Lys Tyr Pro Leu Ala Asn Asn Pro Tyr 
            20                  25                  30          


Ser Ser Ala Leu Asn Leu Asn Ser Cys Gln Asn Ser Ser Ile Leu Asn 
        35                  40                  45              


Trp Ile Asn Ile Ile Gly Asp Ala Ala Lys Glu Ala Val Ser Ile Gly 
    50                  55                  60                  


Thr Thr Ile Val Ser Leu Ile Thr Ala Pro Ser Leu Thr Gly Leu Ile 
65                  70                  75                  80  


Ser Ile Val Tyr Asp Leu Ile Lys Lys Val Leu Gly Gly Ser Ser Gly 
                85                  90                  95      


Gln Ser Ile Ser Asp Leu Ser Ile Cys Asp Leu Leu Ser Ile Ile Asp 
            100                 105                 110         


Leu Arg Val Ser Gln Ser Val Leu Asn Asp Gly Ile Ala Asp Phe Asn 
        115                 120                 125             


Gly Ser Val Leu Leu Tyr Arg Asn Tyr Leu Glu Ala Leu Asp Ser Trp 
    130                 135                 140                 


Asn Lys Asn Pro Asn Ser Ala Ser Ala Glu Glu Leu Arg Thr Arg Phe 
145                 150                 155                 160 


Arg Ile Ala Asp Ser Glu Phe Asp Arg Ile Leu Thr Arg Gly Ser Leu 
                165                 170                 175     


Thr Asn Gly Gly Ser Leu Ala Arg Gln Asn Ala Gln Ile Leu Leu Leu 
            180                 185                 190         


Pro Ser Phe Ala Ser Ala Ala Phe Phe His Leu Leu Leu Leu Arg Asp 
        195                 200                 205             


Ala Thr Arg Tyr Gly Thr Asn Trp Gly Leu Tyr Asn Ala Thr Pro Phe 
    210                 215                 220                 


Ile Asn Tyr Gln Ser Lys Leu Val Glu Leu Ile Glu Leu Tyr Thr Asp 
225                 230                 235                 240 


Tyr Cys Val His Trp Tyr Asn Arg Gly Phe Asn Glu Leu Arg Gln Arg 
                245                 250                 255     


Gly Thr Ser Ala Thr Ala Trp Leu Glu Phe His Arg Tyr Arg Arg Glu 
            260                 265                 270         


Met Thr Leu Met Val Leu Asp Ile Val Ala Ser Phe Ser Ser Leu Asp 
        275                 280                 285             


Ile Thr Asn Tyr Pro Ile Glu Thr Asp Phe Gln Leu Ser Arg Val Ile 
    290                 295                 300                 


Tyr Thr Asp Pro Ile Gly Phe Val His Arg Ser Ser Leu Arg Gly Glu 
305                 310                 315                 320 


Ser Trp Phe Ser Phe Val Asn Arg Ala Asn Phe Ser Asp Leu Glu Asn 
                325                 330                 335     


Ala Ile Pro Asn Pro Arg Pro Ser Trp Phe Leu Asn Asn Met Ile Ile 
            340                 345                 350         


Ser Thr Gly Ser Leu Thr Leu Pro Val Ser Pro Ser Thr Asp Arg Ala 
        355                 360                 365             


Arg Val Leu Tyr Gly Ser Arg Asp Arg Ile Ser Pro Ala Asn Ser Gln 
    370                 375                 380                 


Phe Ile Thr Glu Leu Ile Ser Gly Gln His Thr Thr Ala Thr Gln Thr 
385                 390                 395                 400 


Ile Leu Gly Arg Asn Ile Phe Arg Val Asp Ser Gln Ala Cys Asn Leu 
                405                 410                 415     


Asn Asp Thr Thr Tyr Gly Val Asn Arg Ala Val Phe Tyr His Asp Ala 
            420                 425                 430         


Ser Glu Gly Ser Gln Arg Ser Val Tyr Glu Gly Tyr Ile Arg Thr Thr 
        435                 440                 445             


Gly Ile Asp Asn Pro Arg Val Gln Asn Ile Asn Thr Tyr Leu Pro Gly 
    450                 455                 460                 


Glu Asn Ser Asp Ile Pro Thr Pro Glu Asp Tyr Thr His Ile Leu Ser 
465                 470                 475                 480 


Thr Thr Ile Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Ser Asn Arg 
                485                 490                 495     


Arg Ser Ser Leu Val Met Tyr Gly Trp Thr His Lys Ser Leu Ala Arg 
            500                 505                 510         


Asn Asn Thr Ile Asn Pro Asp Arg Ile Thr Gln Ile Pro Leu Thr Lys 
        515                 520                 525             


Val Asp Thr Arg Gly Thr Gly Val Ser Tyr Val Asn Asp Pro Gly Phe 
    530                 535                 540                 


Ile Gly Gly Ala Leu Leu Gln Arg Thr Asp Asn Gly Ser Leu Gly Val 
545                 550                 555                 560 


Leu Arg Val Gln Phe Pro Leu His Leu Arg Gln Gln Tyr Arg Ile Arg 
                565                 570                 575     


Val Arg Tyr Ala Ser Thr Thr Asn Ile Gln Leu Ser Val Asn Gly Ser 
            580                 585                 590         


Phe Gly Thr Ile Ser Gln Asn Leu Pro Ser Thr Met Arg Leu Gly Glu 
        595                 600                 605             


Asp Leu Arg Tyr Gly Ser Phe Ala Ile Arg Glu Phe Ser Thr Ser Ile 
    610                 615                 620                 


Arg Pro Thr Ala Ser Pro Asp Gln Ile Arg Leu Thr Ile Glu Pro Ser 
625                 630                 635                 640 


Phe Ile Arg Gln Glu Val Tyr Val Asp Arg Ile Glu Phe Ile Pro Val 
                645                 650                 655     


Asn Pro Thr Arg Glu Ala Lys Glu Asp Leu Glu Ala Thr Lys Lys Ala 
            660                 665                 670         


Val Ala Ser Leu Phe Thr Arg Thr Arg Asp Gly Leu Gln Val Asn Val 
        675                 680                 685             


Thr Asp Tyr Gln Val Asp Gln Ala Ala Asn Leu Val Ser Cys Leu Ser 
    690                 695                 700                 


Asp Glu Gln Tyr Ala His Asp Lys Lys Met Leu Leu Glu Ala Val Arg 
705                 710                 715                 720 


Ala Ala Lys Arg Leu Ser Arg Glu Arg Asn Leu Leu Gln Asp Pro Asp 
                725                 730                 735     


Phe Asn Thr Ile Asn Ser Thr Glu Glu Asn Gly Trp Lys Ala Ser Asn 
            740                 745                 750         


Gly Val Thr Ile Ser Glu Gly Gly Pro Phe Tyr Lys Gly Arg Ala Leu 
        755                 760                 765             


Gln Leu Ala Ser Ala Arg Glu Asn Tyr Pro Thr Tyr Ile Tyr Gln Lys 
    770                 775                 780                 


Val Asp Ala Ser Glu Leu Lys Pro Tyr Thr Arg Tyr Arg Leu Asp Gly 
785                 790                 795                 800 


Phe Val Lys Ser Ser Gln Asp Leu Glu Ile Asp Leu Ile His His His 
                805                 810                 815     


Lys Val His Leu Val Lys Asn Ala Pro Asp Asn Leu Val Ser Asp Thr 
            820                 825                 830         


Tyr Pro Asp Asp Ser Cys Ser Gly Ile Asn Arg Cys Gln Glu Gln Gln 
        835                 840                 845             


Met Val Asn Ala Gln Leu Glu Thr Glu His His His Pro Met Asp Cys 
    850                 855                 860                 


Cys Gly Ala Ala Gln Thr His Glu Phe Ser Ser Tyr Ile Asp Thr Gly 
865                 870                 875                 880 


Asp Leu Asn Ser Thr Val Asp Gln Gly Ile Trp Ala Ile Phe Lys Val 
                885                 890                 895     


Arg Thr Thr Asp Gly Tyr Ala Thr Leu Gly Asn Leu Glu Leu Val Glu 
            900                 905                 910         


Ile Gly Pro Leu Ser Gly Glu Ser Leu Glu Arg Glu Gln Arg Asp Asn 
        915                 920                 925             


Ala Lys Trp Ser Ala Glu Leu Gly Arg Lys Arg Ala Glu Thr Asp Arg 
    930                 935                 940                 


Val Tyr Gln Asp Ala Lys Gln Ser Ile Asn His Leu Phe Val Asp Tyr 
945                 950                 955                 960 


Gln Asp Gln Gln Leu Asn Pro Glu Ile Gly Met Ala Asp Ile Met Asp 
                965                 970                 975     


Ala Gln Asn Leu Val Ala Ser Ile Ser Asp Val Tyr Ser Asp Ala Val 
            980                 985                 990         


Leu Gln Ile Pro Gly Ile Asn Tyr  Glu Ile Tyr Thr Glu  Leu Ser Asn 
        995                 1000                 1005             


Arg Leu  Gln Gln Ala Ser Tyr  Leu Tyr Thr Ser Arg  Asn Ala Val 
    1010                 1015                 1020             


Gln Asn  Gly Asp Phe Asn Ser  Gly Leu Asp Ser Trp  Asn Ala Thr 
    1025                 1030                 1035             


Ala Gly  Ala Thr Val Gln Gln  Asp Gly Asn Thr His  Phe Leu Val 
    1040                 1045                 1050             


Leu Ser  His Trp Asp Ala Gln  Val Ser Gln Gln Phe  Arg Val Gln 
    1055                 1060                 1065             


Pro Asn  Cys Lys Tyr Val Leu  Arg Val Thr Ala Glu  Lys Val Gly 
    1070                 1075                 1080             


Gly Gly  Asp Gly Tyr Val Thr  Ile Arg Asp Gly Ala  His His Thr 
    1085                 1090                 1095             


Glu Thr  Leu Thr Phe Asn Ala  Cys Asp Tyr Asp Ile  Asn Gly Thr 
    1100                 1105                 1110             


Tyr Val  Thr Asp Asn Thr Tyr  Leu Thr Lys Glu Val  Val Phe Tyr 
    1115                 1120                 1125             


Ser His  Thr Glu His Met Trp  Val Glu Val Ser Glu  Thr Glu Gly 
    1130                 1135                 1140             


Val Phe  His Ile Asp Ser Val  Glu Phe Met Glu Thr  Gln Gln 
    1145                 1150                 1155         


<210>  31
<211>  1992
<212>  DNA
<213>  Artificial

<220>
<223>  Plant synthetic coding sequence encoding a TIC4064_19 pesticidal 
       protein with an additional alanine codon inserted immediately 
       following the initiating methionine and comprising a protoxin 
       doamin truncation and mutations at G88K, W371L, H555N, and R586Q.

<400>  31
atggctaacc agaacaagca cggcatcatt ggcgcatcca actgcgggtg taccagtgac       60

aacgtggcga aatacccgct cgccaataac ccatactcat cagccctgaa cctcaacagt      120

tgtcagaaca gctccatcct gaattggatc aacatcatcg gcgacgcggc gaaggaggcg      180

gtgagcatcg ggacaaccat cgtttcgctt atcacagctc cctcgctgac gggcctcatc      240

tcaatcgttt atgacctcat taagaaggtg ctcggcggct cgtccggcca atccattagc      300

gacctttcca tctgcgatct gctctccata attgacctgc gggtgtcgca gtcggtcctg      360

aacgacggga tagcggactt caacggcagc gtcctcctgt accgcaacta cctggaggca      420

ctggactcct ggaacaagaa tcccaacagc gccagcgcgg aggagctgcg gacgcgcttt      480

cgcatcgctg attctgagtt tgaccgaatc ctgacacgcg gctcccttac caacggcggt      540

agcctggctc gccagaacgc ccagattctg ttgctgccca gtttcgcgtc agccgcattc      600

tttcacctac ttctccttcg ggacgcaacg cgctacggga ccaattgggg attgtacaac      660

gcgacgccct tcatcaacta tcagtctaag ctcgtcgaac tgatcgaact ctacacggac      720

tactgcgtcc actggtacaa tcgtggcttt aacgagttgc gtcaacgcgg aacttctgct      780

acggcgtggc ttgagtttca cagataccgt cgtgagatga cgctaatggt cctcgacatc      840

gtggcgtcgt tcagctcgct tgacatcacc aactacccta tcgagaccga ctttcaactt      900

agccgtgtga tctacactga cccaatcggc ttcgtccatc gaagttcact ccgtggcgag      960

tcctggttct cgttcgtgaa ccgcgccaac ttcagtgacc ttgagaatgc gatccctaac     1020

ccacgcccta gctggttcct gaacaacatg attatctcta cagggagcct gaccttaccc     1080

gtcagtccca gcacagatcg cgcgagagtc ttgtacggga gtcgggaccg gatctcgccc     1140

gccaactctc agttcattac tgagcttatc tccgggcagc acaccaccgc cacccagaca     1200

atactcggtc gcaacatctt ccgcgtggat tcacaggcgt gcaacctaaa cgacaccacg     1260

tacggcgtca accgcgccgt gttctaccat gacgcctcgg agggctcgca gcgtagcgtt     1320

tatgagggct acatcaggac gacgggtatc gacaacccga gagtgcagaa catcaacacc     1380

tatttgccag gcgagaacag cgacatcccg acgcccgaag actacaccca catcctcagc     1440

acaaccatca acctcacggg cgggctccgc caagttgcct cgaaccggcg gagcagcctc     1500

gttatgtacg gctggaccca caagtctctg gcgcgcaaca acaccatcaa tccagatcga     1560

atcacccaga taccactcac gaaggtggat acccgaggga ctggcgttag ttacgtcaac     1620

gatcctggat tcatcggcgg cgcgcttctc caacgcacgg acaacgggtc gctgggcgtc     1680

cttcgggtac agtttccgct acacttgcgc cagcaatacc ggataagggt tcggtacgcc     1740

tcaacaacga acatccaact gtctgtcaac ggctccttcg gcaccatctc ccagaacctt     1800

ccctccacca tgcgcctcgg tgaggatctg cgctacggct ctttcgctat ccgcgagttt     1860

agcacttcaa tacgcccaac cgcgtcacct gaccagatac gcctgaccat tgaaccgtcc     1920

ttcatccggc aagaggtgta cgtggacaga atcgagttca taccagtcaa cccgacgcgg     1980

gaggcgaagt ga                                                         1992


<210>  32
<211>  663
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of TIC4064_19 with an additional alanine 
       amino acid inserted immediately following the initiating 
       methionine and comprising a protoxin domain truncation and 
       mutations at G88K, W371L, H555N, and R586Q.

<400>  32

Met Ala Asn Gln Asn Lys His Gly Ile Ile Gly Ala Ser Asn Cys Gly 
1               5                   10                  15      


Cys Thr Ser Asp Asn Val Ala Lys Tyr Pro Leu Ala Asn Asn Pro Tyr 
            20                  25                  30          


Ser Ser Ala Leu Asn Leu Asn Ser Cys Gln Asn Ser Ser Ile Leu Asn 
        35                  40                  45              


Trp Ile Asn Ile Ile Gly Asp Ala Ala Lys Glu Ala Val Ser Ile Gly 
    50                  55                  60                  


Thr Thr Ile Val Ser Leu Ile Thr Ala Pro Ser Leu Thr Gly Leu Ile 
65                  70                  75                  80  


Ser Ile Val Tyr Asp Leu Ile Lys Lys Val Leu Gly Gly Ser Ser Gly 
                85                  90                  95      


Gln Ser Ile Ser Asp Leu Ser Ile Cys Asp Leu Leu Ser Ile Ile Asp 
            100                 105                 110         


Leu Arg Val Ser Gln Ser Val Leu Asn Asp Gly Ile Ala Asp Phe Asn 
        115                 120                 125             


Gly Ser Val Leu Leu Tyr Arg Asn Tyr Leu Glu Ala Leu Asp Ser Trp 
    130                 135                 140                 


Asn Lys Asn Pro Asn Ser Ala Ser Ala Glu Glu Leu Arg Thr Arg Phe 
145                 150                 155                 160 


Arg Ile Ala Asp Ser Glu Phe Asp Arg Ile Leu Thr Arg Gly Ser Leu 
                165                 170                 175     


Thr Asn Gly Gly Ser Leu Ala Arg Gln Asn Ala Gln Ile Leu Leu Leu 
            180                 185                 190         


Pro Ser Phe Ala Ser Ala Ala Phe Phe His Leu Leu Leu Leu Arg Asp 
        195                 200                 205             


Ala Thr Arg Tyr Gly Thr Asn Trp Gly Leu Tyr Asn Ala Thr Pro Phe 
    210                 215                 220                 


Ile Asn Tyr Gln Ser Lys Leu Val Glu Leu Ile Glu Leu Tyr Thr Asp 
225                 230                 235                 240 


Tyr Cys Val His Trp Tyr Asn Arg Gly Phe Asn Glu Leu Arg Gln Arg 
                245                 250                 255     


Gly Thr Ser Ala Thr Ala Trp Leu Glu Phe His Arg Tyr Arg Arg Glu 
            260                 265                 270         


Met Thr Leu Met Val Leu Asp Ile Val Ala Ser Phe Ser Ser Leu Asp 
        275                 280                 285             


Ile Thr Asn Tyr Pro Ile Glu Thr Asp Phe Gln Leu Ser Arg Val Ile 
    290                 295                 300                 


Tyr Thr Asp Pro Ile Gly Phe Val His Arg Ser Ser Leu Arg Gly Glu 
305                 310                 315                 320 


Ser Trp Phe Ser Phe Val Asn Arg Ala Asn Phe Ser Asp Leu Glu Asn 
                325                 330                 335     


Ala Ile Pro Asn Pro Arg Pro Ser Trp Phe Leu Asn Asn Met Ile Ile 
            340                 345                 350         


Ser Thr Gly Ser Leu Thr Leu Pro Val Ser Pro Ser Thr Asp Arg Ala 
        355                 360                 365             


Arg Val Leu Tyr Gly Ser Arg Asp Arg Ile Ser Pro Ala Asn Ser Gln 
    370                 375                 380                 


Phe Ile Thr Glu Leu Ile Ser Gly Gln His Thr Thr Ala Thr Gln Thr 
385                 390                 395                 400 


Ile Leu Gly Arg Asn Ile Phe Arg Val Asp Ser Gln Ala Cys Asn Leu 
                405                 410                 415     


Asn Asp Thr Thr Tyr Gly Val Asn Arg Ala Val Phe Tyr His Asp Ala 
            420                 425                 430         


Ser Glu Gly Ser Gln Arg Ser Val Tyr Glu Gly Tyr Ile Arg Thr Thr 
        435                 440                 445             


Gly Ile Asp Asn Pro Arg Val Gln Asn Ile Asn Thr Tyr Leu Pro Gly 
    450                 455                 460                 


Glu Asn Ser Asp Ile Pro Thr Pro Glu Asp Tyr Thr His Ile Leu Ser 
465                 470                 475                 480 


Thr Thr Ile Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Ser Asn Arg 
                485                 490                 495     


Arg Ser Ser Leu Val Met Tyr Gly Trp Thr His Lys Ser Leu Ala Arg 
            500                 505                 510         


Asn Asn Thr Ile Asn Pro Asp Arg Ile Thr Gln Ile Pro Leu Thr Lys 
        515                 520                 525             


Val Asp Thr Arg Gly Thr Gly Val Ser Tyr Val Asn Asp Pro Gly Phe 
    530                 535                 540                 


Ile Gly Gly Ala Leu Leu Gln Arg Thr Asp Asn Gly Ser Leu Gly Val 
545                 550                 555                 560 


Leu Arg Val Gln Phe Pro Leu His Leu Arg Gln Gln Tyr Arg Ile Arg 
                565                 570                 575     


Val Arg Tyr Ala Ser Thr Thr Asn Ile Gln Leu Ser Val Asn Gly Ser 
            580                 585                 590         


Phe Gly Thr Ile Ser Gln Asn Leu Pro Ser Thr Met Arg Leu Gly Glu 
        595                 600                 605             


Asp Leu Arg Tyr Gly Ser Phe Ala Ile Arg Glu Phe Ser Thr Ser Ile 
    610                 615                 620                 


Arg Pro Thr Ala Ser Pro Asp Gln Ile Arg Leu Thr Ile Glu Pro Ser 
625                 630                 635                 640 


Phe Ile Arg Gln Glu Val Tyr Val Asp Arg Ile Glu Phe Ile Pro Val 
                645                 650                 655     


Asn Pro Thr Arg Glu Ala Lys 
            660             


<210>  33
<211>  3471
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic bacterial coding sequence encoding TIC4064_20 
       comprising mutations at S94T, D84A, A510H, N512D, and D608A.

<400>  33
atgaatcaaa ataaacacgg aattattggc gcttccaatt gtggttgtac gtcagataat       60

gttgcgaaat atcctttagc caacaatcca tattcatctg ctttaaattt aaattcttgt      120

caaaatagta gtattctcaa ctggattaac ataataggcg atgcagcaaa agaagcagta      180

tctattggga caacaatagt ctctcttatc acagcacctt ctcttactgg attaatttca      240

atagtatatg cacttatagg taaagtacta ggaggtagta caggacaatc catatcagat      300

ttgtctatat gtgacttatt atctattatt gatttacggg taagtcagag tgttttaaat      360

gatgggattg cagattttaa tggttctgta ctcttataca ggaactattt agaggctctg      420

gatagctgga ataagaatcc taattctgct tctgctgaag aactccgtac tcgttttaga      480

atcgctgact cagaatttga tagaatttta acacgagggt ctttaacgaa tggtggctcg      540

ttagctagac aaaatgccca aatattatta ttaccttctt ttgcgagtgc tgcatttttc      600

catttattac tactaaggga tgctactaga tatggcacta attgggggct atacaatgct      660

acacctttta taaattatca atcaaaacta gtagagctta ttgaactata tactgattat      720

tgcgtacatt ggtataatcg aggtttcaac gaactaaggc aacgaggcac tagtgctaca      780

gcttggttag aatttcatag atatcgtaga gagatgacat tgatggtatt agatatagta      840

gcatcatttt caagtcttga tattactaat tacccaatag aaacagattt tcagttgagt      900

agggtcattt atacagatcc aattggtttt gtacatcgta gtagtcttag gggagaaagt      960

tggtttagct ttgttaatag agctaatttc tcagatttag aaaatgcaat acctaatcct     1020

agaccgtctt ggtttttaaa taatatgatt atatctactg gttcacttac attgccggtt     1080

agcccaagta ctgatagagc gagggtatgg tatggaagtc gagatcgaat ttcccctgct     1140

aattcacaat ttattactga actaatctct ggacaacata cgactgctac acaaactatt     1200

ttagggcgaa atatatttag agtagattct caagcttgta atttaaatga taccacatat     1260

ggagtgaata gggcggtatt ttatcatgat gcgagtgaag gttctcaaag atccgtgtac     1320

gaggggtata ttcgaacaac tgggatagat aaccctagag ttcaaaatat taacacttat     1380

ttacctggag aaaattcaga tatcccaact ccagaagact atactcatat attaagcaca     1440

acaataaatt taacaggagg acttagacaa gtagcatcta atcgccgttc atctttagta     1500

atgtatggtt ggacacataa aagtctgcat cgtgataata ccattaatcc agatagaatt     1560

acacagatac cattgacgaa ggttgatacc cgaggcacag gtgtttctta tgtgaatgat     1620

ccaggattta taggaggagc tctacttcaa aggactgacc atggttcgct tggagtattg     1680

agggtccaat ttccacttca cttaagacaa caatatcgca ttagagtccg ttatgcttct     1740

acaacaaata ttcgattgag tgtgaatggc agtttcggta ctatttctca aaatctccct     1800

agtacaatga gattaggaga ggcattaaga tacggatctt ttgctataag agagtttagt     1860

acttctatta gacccactgc aagtccggac caaattcgat tgacaataga accatctttt     1920

attagacaag aggtctatgt agatagaatt gagttcattc cagttaatcc gacgcgagag     1980

gcgaaagagg atctagaagc aacaaagaaa gcggtggcga gcttgtttac acgcacaagg     2040

gacggattac aagtaaatgt gacagattat caagtcgatc aagcggcaaa tttagtgtca     2100

tgcttatcag atgaacaata tgcgcatgat aaaaagatgt tattggaagc ggtacgcgcg     2160

gcaaaacgcc tcagccgaga acgcaactta cttcaggatc cagattttaa tacaatcaat     2220

agtacagaag aaaatggatg gaaagcaagt aacggcgtta ctattagcga aggcggtcca     2280

ttctataaag gccgtgcact tcagctagca agtgcacgag aaaattaccc aacatacatc     2340

tatcaaaaag tagatgcatc ggagttaaag ccgtatacac gttatagact ggatgggttc     2400

gtgaagagta gtcaagattt agaaattgat ctcattcacc atcataaagt ccatcttgtg     2460

aaaaatgcac cagataattt agtatctgat acttacccag atgattcttg tagtggaatc     2520

aatcgatgtc aggaacaaca gatggtaaat gcgcaactgg aaacagaaca tcatcatccg     2580

atggattgct gtggagcagc tcaaacacat gagttttctt cctatattga tacaggggat     2640

ttaaattcga ctgtagacca gggaatctgg gcgatcttta aagttcgaac aacagatggt     2700

tatgcgacgt taggaaatct tgaattggta gagatcggac cgttatcggg tgaatctcta     2760

gaacgtgaac aaagggataa tgcaaaatgg agtgcagagc taggaagaaa gcgtgcagaa     2820

acagatcgcg tgtatcaaga tgccaaacaa tccatcaatc atttatttgt ggattatcaa     2880

gatcaacaat taaatccaga aatagggatg gcagatatta tggacgctca aaatcttgtc     2940

gcatcaattt cagatgtata tagcgatgca gtactgcaaa tccctggaat taactatgag     3000

atttacacag agctatccaa tcgcttacaa caagcatcgt atctgtatac gtctcgaaat     3060

gcggtgcaaa atggggactt taacagcggt ctagatagtt ggaatgcaac agcgggtgct     3120

acggtacaac aggatggtaa tacgcatttc ttagttcttt ctcattggga tgcacaagtt     3180

tctcaacaat ttagagtgca gccaaattgt aaatatgtat tacgtgtaac agcagagaaa     3240

gtaggcggcg gagacggata cgtgacaatc cgggatggtg ctcatcatac agaaacgctt     3300

acatttaatg catgtgatta tgatataaat ggcacgtacg tgactgataa tacgtatcta     3360

acaaaagaag tggtattcta ttcacataca gaacacatgt gggtagaggt aagtgaaaca     3420

gaaggtgttt tccatataga cagtgttgag ttcatggaaa cccaacagta g              3471


<210>  34
<211>  1156
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of TIC4064_20 comprising mutations at S94T, 
       D84A, A510H, N512D, and D608A.

<400>  34

Met Asn Gln Asn Lys His Gly Ile Ile Gly Ala Ser Asn Cys Gly Cys 
1               5                   10                  15      


Thr Ser Asp Asn Val Ala Lys Tyr Pro Leu Ala Asn Asn Pro Tyr Ser 
            20                  25                  30          


Ser Ala Leu Asn Leu Asn Ser Cys Gln Asn Ser Ser Ile Leu Asn Trp 
        35                  40                  45              


Ile Asn Ile Ile Gly Asp Ala Ala Lys Glu Ala Val Ser Ile Gly Thr 
    50                  55                  60                  


Thr Ile Val Ser Leu Ile Thr Ala Pro Ser Leu Thr Gly Leu Ile Ser 
65                  70                  75                  80  


Ile Val Tyr Ala Leu Ile Gly Lys Val Leu Gly Gly Ser Thr Gly Gln 
                85                  90                  95      


Ser Ile Ser Asp Leu Ser Ile Cys Asp Leu Leu Ser Ile Ile Asp Leu 
            100                 105                 110         


Arg Val Ser Gln Ser Val Leu Asn Asp Gly Ile Ala Asp Phe Asn Gly 
        115                 120                 125             


Ser Val Leu Leu Tyr Arg Asn Tyr Leu Glu Ala Leu Asp Ser Trp Asn 
    130                 135                 140                 


Lys Asn Pro Asn Ser Ala Ser Ala Glu Glu Leu Arg Thr Arg Phe Arg 
145                 150                 155                 160 


Ile Ala Asp Ser Glu Phe Asp Arg Ile Leu Thr Arg Gly Ser Leu Thr 
                165                 170                 175     


Asn Gly Gly Ser Leu Ala Arg Gln Asn Ala Gln Ile Leu Leu Leu Pro 
            180                 185                 190         


Ser Phe Ala Ser Ala Ala Phe Phe His Leu Leu Leu Leu Arg Asp Ala 
        195                 200                 205             


Thr Arg Tyr Gly Thr Asn Trp Gly Leu Tyr Asn Ala Thr Pro Phe Ile 
    210                 215                 220                 


Asn Tyr Gln Ser Lys Leu Val Glu Leu Ile Glu Leu Tyr Thr Asp Tyr 
225                 230                 235                 240 


Cys Val His Trp Tyr Asn Arg Gly Phe Asn Glu Leu Arg Gln Arg Gly 
                245                 250                 255     


Thr Ser Ala Thr Ala Trp Leu Glu Phe His Arg Tyr Arg Arg Glu Met 
            260                 265                 270         


Thr Leu Met Val Leu Asp Ile Val Ala Ser Phe Ser Ser Leu Asp Ile 
        275                 280                 285             


Thr Asn Tyr Pro Ile Glu Thr Asp Phe Gln Leu Ser Arg Val Ile Tyr 
    290                 295                 300                 


Thr Asp Pro Ile Gly Phe Val His Arg Ser Ser Leu Arg Gly Glu Ser 
305                 310                 315                 320 


Trp Phe Ser Phe Val Asn Arg Ala Asn Phe Ser Asp Leu Glu Asn Ala 
                325                 330                 335     


Ile Pro Asn Pro Arg Pro Ser Trp Phe Leu Asn Asn Met Ile Ile Ser 
            340                 345                 350         


Thr Gly Ser Leu Thr Leu Pro Val Ser Pro Ser Thr Asp Arg Ala Arg 
        355                 360                 365             


Val Trp Tyr Gly Ser Arg Asp Arg Ile Ser Pro Ala Asn Ser Gln Phe 
    370                 375                 380                 


Ile Thr Glu Leu Ile Ser Gly Gln His Thr Thr Ala Thr Gln Thr Ile 
385                 390                 395                 400 


Leu Gly Arg Asn Ile Phe Arg Val Asp Ser Gln Ala Cys Asn Leu Asn 
                405                 410                 415     


Asp Thr Thr Tyr Gly Val Asn Arg Ala Val Phe Tyr His Asp Ala Ser 
            420                 425                 430         


Glu Gly Ser Gln Arg Ser Val Tyr Glu Gly Tyr Ile Arg Thr Thr Gly 
        435                 440                 445             


Ile Asp Asn Pro Arg Val Gln Asn Ile Asn Thr Tyr Leu Pro Gly Glu 
    450                 455                 460                 


Asn Ser Asp Ile Pro Thr Pro Glu Asp Tyr Thr His Ile Leu Ser Thr 
465                 470                 475                 480 


Thr Ile Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Ser Asn Arg Arg 
                485                 490                 495     


Ser Ser Leu Val Met Tyr Gly Trp Thr His Lys Ser Leu His Arg Asp 
            500                 505                 510         


Asn Thr Ile Asn Pro Asp Arg Ile Thr Gln Ile Pro Leu Thr Lys Val 
        515                 520                 525             


Asp Thr Arg Gly Thr Gly Val Ser Tyr Val Asn Asp Pro Gly Phe Ile 
    530                 535                 540                 


Gly Gly Ala Leu Leu Gln Arg Thr Asp His Gly Ser Leu Gly Val Leu 
545                 550                 555                 560 


Arg Val Gln Phe Pro Leu His Leu Arg Gln Gln Tyr Arg Ile Arg Val 
                565                 570                 575     


Arg Tyr Ala Ser Thr Thr Asn Ile Arg Leu Ser Val Asn Gly Ser Phe 
            580                 585                 590         


Gly Thr Ile Ser Gln Asn Leu Pro Ser Thr Met Arg Leu Gly Glu Ala 
        595                 600                 605             


Leu Arg Tyr Gly Ser Phe Ala Ile Arg Glu Phe Ser Thr Ser Ile Arg 
    610                 615                 620                 


Pro Thr Ala Ser Pro Asp Gln Ile Arg Leu Thr Ile Glu Pro Ser Phe 
625                 630                 635                 640 


Ile Arg Gln Glu Val Tyr Val Asp Arg Ile Glu Phe Ile Pro Val Asn 
                645                 650                 655     


Pro Thr Arg Glu Ala Lys Glu Asp Leu Glu Ala Thr Lys Lys Ala Val 
            660                 665                 670         


Ala Ser Leu Phe Thr Arg Thr Arg Asp Gly Leu Gln Val Asn Val Thr 
        675                 680                 685             


Asp Tyr Gln Val Asp Gln Ala Ala Asn Leu Val Ser Cys Leu Ser Asp 
    690                 695                 700                 


Glu Gln Tyr Ala His Asp Lys Lys Met Leu Leu Glu Ala Val Arg Ala 
705                 710                 715                 720 


Ala Lys Arg Leu Ser Arg Glu Arg Asn Leu Leu Gln Asp Pro Asp Phe 
                725                 730                 735     


Asn Thr Ile Asn Ser Thr Glu Glu Asn Gly Trp Lys Ala Ser Asn Gly 
            740                 745                 750         


Val Thr Ile Ser Glu Gly Gly Pro Phe Tyr Lys Gly Arg Ala Leu Gln 
        755                 760                 765             


Leu Ala Ser Ala Arg Glu Asn Tyr Pro Thr Tyr Ile Tyr Gln Lys Val 
    770                 775                 780                 


Asp Ala Ser Glu Leu Lys Pro Tyr Thr Arg Tyr Arg Leu Asp Gly Phe 
785                 790                 795                 800 


Val Lys Ser Ser Gln Asp Leu Glu Ile Asp Leu Ile His His His Lys 
                805                 810                 815     


Val His Leu Val Lys Asn Ala Pro Asp Asn Leu Val Ser Asp Thr Tyr 
            820                 825                 830         


Pro Asp Asp Ser Cys Ser Gly Ile Asn Arg Cys Gln Glu Gln Gln Met 
        835                 840                 845             


Val Asn Ala Gln Leu Glu Thr Glu His His His Pro Met Asp Cys Cys 
    850                 855                 860                 


Gly Ala Ala Gln Thr His Glu Phe Ser Ser Tyr Ile Asp Thr Gly Asp 
865                 870                 875                 880 


Leu Asn Ser Thr Val Asp Gln Gly Ile Trp Ala Ile Phe Lys Val Arg 
                885                 890                 895     


Thr Thr Asp Gly Tyr Ala Thr Leu Gly Asn Leu Glu Leu Val Glu Ile 
            900                 905                 910         


Gly Pro Leu Ser Gly Glu Ser Leu Glu Arg Glu Gln Arg Asp Asn Ala 
        915                 920                 925             


Lys Trp Ser Ala Glu Leu Gly Arg Lys Arg Ala Glu Thr Asp Arg Val 
    930                 935                 940                 


Tyr Gln Asp Ala Lys Gln Ser Ile Asn His Leu Phe Val Asp Tyr Gln 
945                 950                 955                 960 


Asp Gln Gln Leu Asn Pro Glu Ile Gly Met Ala Asp Ile Met Asp Ala 
                965                 970                 975     


Gln Asn Leu Val Ala Ser Ile Ser Asp Val Tyr Ser Asp Ala Val Leu 
            980                 985                 990         


Gln Ile Pro Gly Ile Asn Tyr Glu  Ile Tyr Thr Glu Leu  Ser Asn Arg 
        995                 1000                 1005             


Leu Gln  Gln Ala Ser Tyr Leu  Tyr Thr Ser Arg Asn  Ala Val Gln 
    1010                 1015                 1020             


Asn Gly  Asp Phe Asn Ser Gly  Leu Asp Ser Trp Asn  Ala Thr Ala 
    1025                 1030                 1035             


Gly Ala  Thr Val Gln Gln Asp  Gly Asn Thr His Phe  Leu Val Leu 
    1040                 1045                 1050             


Ser His  Trp Asp Ala Gln Val  Ser Gln Gln Phe Arg  Val Gln Pro 
    1055                 1060                 1065             


Asn Cys  Lys Tyr Val Leu Arg  Val Thr Ala Glu Lys  Val Gly Gly 
    1070                 1075                 1080             


Gly Asp  Gly Tyr Val Thr Ile  Arg Asp Gly Ala His  His Thr Glu 
    1085                 1090                 1095             


Thr Leu  Thr Phe Asn Ala Cys  Asp Tyr Asp Ile Asn  Gly Thr Tyr 
    1100                 1105                 1110             


Val Thr  Asp Asn Thr Tyr Leu  Thr Lys Glu Val Val  Phe Tyr Ser 
    1115                 1120                 1125             


His Thr  Glu His Met Trp Val  Glu Val Ser Glu Thr  Glu Gly Val 
    1130                 1135                 1140             


Phe His  Ile Asp Ser Val Glu  Phe Met Glu Thr Gln  Gln 
    1145                 1150                 1155     


<210>  35
<211>  3471
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic bacterial coding sequence encoding TIC4064_21 
       comprising mutations at S94T, R168K, and S331A.

<400>  35
atgaatcaaa ataaacacgg aattattggc gcttccaatt gtggttgtac gtcagataat       60

gttgcgaaat atcctttagc caacaatcca tattcatctg ctttaaattt aaattcttgt      120

caaaatagta gtattctcaa ctggattaac ataataggcg atgcagcaaa agaagcagta      180

tctattggga caacaatagt ctctcttatc acagcacctt ctcttactgg attaatttca      240

atagtatatg cacttatagg taaagtacta ggaggtagta caggacaatc catatcagat      300

ttgtctatat gtgacttatt atctattatt gatttacggg taagtcagag tgttttaaat      360

gatgggattg cagattttaa tggttctgta ctcttataca ggaactattt agaggctctg      420

gatagctgga ataagaatcc taattctgct tctgctgaag aactccgtac tcgttttaga      480

atcgctgact cagaatttga tagaatttta acacgagggt ctttaacgaa tggtggctcg      540

ttagctagac aaaatgccca aatattatta ttaccttctt ttgcgagtgc tgcatttttc      600

catttattac tactaaggga tgctactaga tatggcacta attgggggct atacaatgct      660

acacctttta taaattatca atcaaaacta gtagagctta ttgaactata tactgattat      720

tgcgtacatt ggtataatcg aggtttcaac gaactaaggc aacgaggcac tagtgctaca      780

gcttggttag aatttcatag atatcgtaga gagatgacat tgatggtatt agatatagta      840

gcatcatttt caagtcttga tattactaat tacccaatag aaacagattt tcagttgagt      900

agggtcattt atacagatcc aattggtttt gtacatcgta gtagtcttag gggagaaagt      960

tggtttagct ttgttaatag agctaatttc tcagatttag aaaatgcaat acctaatcct     1020

agaccgtctt ggtttttaaa taatatgatt atatctactg gttcacttac attgccggtt     1080

agcccaagta ctgatagagc gagggtatgg tatggaagtc gagatcgaat ttcccctgct     1140

aattcacaat ttattactga actaatctct ggacaacata cgactgctac acaaactatt     1200

ttagggcgaa atatatttag agtagattct caagcttgta atttaaatga taccacatat     1260

ggagtgaata gggcggtatt ttatcatgat gcgagtgaag gttctcaaag atccgtgtac     1320

gaggggtata ttcgaacaac tgggatagat aaccctagag ttcaaaatat taacacttat     1380

ttacctggag aaaattcaga tatcccaact ccagaagact atactcatat attaagcaca     1440

acaataaatt taacaggagg acttagacaa gtagcatcta atcgccgttc atctttagta     1500

atgtatggtt ggacacataa aagtctgcat cgtgataata ccattaatcc agatagaatt     1560

acacagatac cattgacgaa ggttgatacc cgaggcacag gtgtttctta tgtgaatgat     1620

ccaggattta taggaggagc tctacttcaa aggactgacc atggttcgct tggagtattg     1680

agggtccaat ttccacttca cttaagacaa caatatcgca ttagagtccg ttatgcttct     1740

acaacaaata ttcgattgag tgtgaatggc agtttcggta ctatttctca aaatctccct     1800

agtacaatga gattaggaga ggcattaaga tacggatctt ttgctataag agagtttagt     1860

acttctatta gacccactgc aagtccggac caaattcgat tgacaataga accatctttt     1920

attagacaag aggtctatgt agatagaatt gagttcattc cagttaatcc gacgcgagag     1980

gcgaaagagg atctagaagc aacaaagaaa gcggtggcga gcttgtttac acgcacaagg     2040

gacggattac aagtaaatgt gacagattat caagtcgatc aagcggcaaa tttagtgtca     2100

tgcttatcag atgaacaata tgcgcatgat aaaaagatgt tattggaagc ggtacgcgcg     2160

gcaaaacgcc tcagccgaga acgcaactta cttcaggatc cagattttaa tacaatcaat     2220

agtacagaag aaaatggatg gaaagcaagt aacggcgtta ctattagcga aggcggtcca     2280

ttctataaag gccgtgcact tcagctagca agtgcacgag aaaattaccc aacatacatc     2340

tatcaaaaag tagatgcatc ggagttaaag ccgtatacac gttatagact ggatgggttc     2400

gtgaagagta gtcaagattt agaaattgat ctcattcacc atcataaagt ccatcttgtg     2460

aaaaatgcac cagataattt agtatctgat acttacccag atgattcttg tagtggaatc     2520

aatcgatgtc aggaacaaca gatggtaaat gcgcaactgg aaacagaaca tcatcatccg     2580

atggattgct gtggagcagc tcaaacacat gagttttctt cctatattga tacaggggat     2640

ttaaattcga ctgtagacca gggaatctgg gcgatcttta aagttcgaac aacagatggt     2700

tatgcgacgt taggaaatct tgaattggta gagatcggac cgttatcggg tgaatctcta     2760

gaacgtgaac aaagggataa tgcaaaatgg agtgcagagc taggaagaaa gcgtgcagaa     2820

acagatcgcg tgtatcaaga tgccaaacaa tccatcaatc atttatttgt ggattatcaa     2880

gatcaacaat taaatccaga aatagggatg gcagatatta tggacgctca aaatcttgtc     2940

gcatcaattt cagatgtata tagcgatgca gtactgcaaa tccctggaat taactatgag     3000

atttacacag agctatccaa tcgcttacaa caagcatcgt atctgtatac gtctcgaaat     3060

gcggtgcaaa atggggactt taacagcggt ctagatagtt ggaatgcaac agcgggtgct     3120

acggtacaac aggatggtaa tacgcatttc ttagttcttt ctcattggga tgcacaagtt     3180

tctcaacaat ttagagtgca gccaaattgt aaatatgtat tacgtgtaac agcagagaaa     3240

gtaggcggcg gagacggata cgtgacaatc cgggatggtg ctcatcatac agaaacgctt     3300

acatttaatg catgtgatta tgatataaat ggcacgtacg tgactgataa tacgtatcta     3360

acaaaagaag tggtattcta ttcacataca gaacacatgt gggtagaggt aagtgaaaca     3420

gaaggtgttt tccatataga cagtgttgag ttcatggaaa cccaacagta g              3471


<210>  36
<211>  1156
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of TIC4064_21 comprising mutations at S94T, 
       R168K, and S331A.

<400>  36

Met Asn Gln Asn Lys His Gly Ile Ile Gly Ala Ser Asn Cys Gly Cys 
1               5                   10                  15      


Thr Ser Asp Asn Val Ala Lys Tyr Pro Leu Ala Asn Asn Pro Tyr Ser 
            20                  25                  30          


Ser Ala Leu Asn Leu Asn Ser Cys Gln Asn Ser Ser Ile Leu Asn Trp 
        35                  40                  45              


Ile Asn Ile Ile Gly Asp Ala Ala Lys Glu Ala Val Ser Ile Gly Thr 
    50                  55                  60                  


Thr Ile Val Ser Leu Ile Thr Ala Pro Ser Leu Thr Gly Leu Ile Ser 
65                  70                  75                  80  


Ile Val Tyr Ala Leu Ile Gly Lys Val Leu Gly Gly Ser Thr Gly Gln 
                85                  90                  95      


Ser Ile Ser Asp Leu Ser Ile Cys Asp Leu Leu Ser Ile Ile Asp Leu 
            100                 105                 110         


Arg Val Ser Gln Ser Val Leu Asn Asp Gly Ile Ala Asp Phe Asn Gly 
        115                 120                 125             


Ser Val Leu Leu Tyr Arg Asn Tyr Leu Glu Ala Leu Asp Ser Trp Asn 
    130                 135                 140                 


Lys Asn Pro Asn Ser Ala Ser Ala Glu Glu Leu Arg Thr Arg Phe Arg 
145                 150                 155                 160 


Ile Ala Asp Ser Glu Phe Asp Arg Ile Leu Thr Arg Gly Ser Leu Thr 
                165                 170                 175     


Asn Gly Gly Ser Leu Ala Arg Gln Asn Ala Gln Ile Leu Leu Leu Pro 
            180                 185                 190         


Ser Phe Ala Ser Ala Ala Phe Phe His Leu Leu Leu Leu Arg Asp Ala 
        195                 200                 205             


Thr Arg Tyr Gly Thr Asn Trp Gly Leu Tyr Asn Ala Thr Pro Phe Ile 
    210                 215                 220                 


Asn Tyr Gln Ser Lys Leu Val Glu Leu Ile Glu Leu Tyr Thr Asp Tyr 
225                 230                 235                 240 


Cys Val His Trp Tyr Asn Arg Gly Phe Asn Glu Leu Arg Gln Arg Gly 
                245                 250                 255     


Thr Ser Ala Thr Ala Trp Leu Glu Phe His Arg Tyr Arg Arg Glu Met 
            260                 265                 270         


Thr Leu Met Val Leu Asp Ile Val Ala Ser Phe Ser Ser Leu Asp Ile 
        275                 280                 285             


Thr Asn Tyr Pro Ile Glu Thr Asp Phe Gln Leu Ser Arg Val Ile Tyr 
    290                 295                 300                 


Thr Asp Pro Ile Gly Phe Val His Arg Ser Ser Leu Arg Gly Glu Ser 
305                 310                 315                 320 


Trp Phe Ser Phe Val Asn Arg Ala Asn Phe Ser Asp Leu Glu Asn Ala 
                325                 330                 335     


Ile Pro Asn Pro Arg Pro Ser Trp Phe Leu Asn Asn Met Ile Ile Ser 
            340                 345                 350         


Thr Gly Ser Leu Thr Leu Pro Val Ser Pro Ser Thr Asp Arg Ala Arg 
        355                 360                 365             


Val Trp Tyr Gly Ser Arg Asp Arg Ile Ser Pro Ala Asn Ser Gln Phe 
    370                 375                 380                 


Ile Thr Glu Leu Ile Ser Gly Gln His Thr Thr Ala Thr Gln Thr Ile 
385                 390                 395                 400 


Leu Gly Arg Asn Ile Phe Arg Val Asp Ser Gln Ala Cys Asn Leu Asn 
                405                 410                 415     


Asp Thr Thr Tyr Gly Val Asn Arg Ala Val Phe Tyr His Asp Ala Ser 
            420                 425                 430         


Glu Gly Ser Gln Arg Ser Val Tyr Glu Gly Tyr Ile Arg Thr Thr Gly 
        435                 440                 445             


Ile Asp Asn Pro Arg Val Gln Asn Ile Asn Thr Tyr Leu Pro Gly Glu 
    450                 455                 460                 


Asn Ser Asp Ile Pro Thr Pro Glu Asp Tyr Thr His Ile Leu Ser Thr 
465                 470                 475                 480 


Thr Ile Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Ser Asn Arg Arg 
                485                 490                 495     


Ser Ser Leu Val Met Tyr Gly Trp Thr His Lys Ser Leu His Arg Asp 
            500                 505                 510         


Asn Thr Ile Asn Pro Asp Arg Ile Thr Gln Ile Pro Leu Thr Lys Val 
        515                 520                 525             


Asp Thr Arg Gly Thr Gly Val Ser Tyr Val Asn Asp Pro Gly Phe Ile 
    530                 535                 540                 


Gly Gly Ala Leu Leu Gln Arg Thr Asp His Gly Ser Leu Gly Val Leu 
545                 550                 555                 560 


Arg Val Gln Phe Pro Leu His Leu Arg Gln Gln Tyr Arg Ile Arg Val 
                565                 570                 575     


Arg Tyr Ala Ser Thr Thr Asn Ile Arg Leu Ser Val Asn Gly Ser Phe 
            580                 585                 590         


Gly Thr Ile Ser Gln Asn Leu Pro Ser Thr Met Arg Leu Gly Glu Ala 
        595                 600                 605             


Leu Arg Tyr Gly Ser Phe Ala Ile Arg Glu Phe Ser Thr Ser Ile Arg 
    610                 615                 620                 


Pro Thr Ala Ser Pro Asp Gln Ile Arg Leu Thr Ile Glu Pro Ser Phe 
625                 630                 635                 640 


Ile Arg Gln Glu Val Tyr Val Asp Arg Ile Glu Phe Ile Pro Val Asn 
                645                 650                 655     


Pro Thr Arg Glu Ala Lys Glu Asp Leu Glu Ala Thr Lys Lys Ala Val 
            660                 665                 670         


Ala Ser Leu Phe Thr Arg Thr Arg Asp Gly Leu Gln Val Asn Val Thr 
        675                 680                 685             


Asp Tyr Gln Val Asp Gln Ala Ala Asn Leu Val Ser Cys Leu Ser Asp 
    690                 695                 700                 


Glu Gln Tyr Ala His Asp Lys Lys Met Leu Leu Glu Ala Val Arg Ala 
705                 710                 715                 720 


Ala Lys Arg Leu Ser Arg Glu Arg Asn Leu Leu Gln Asp Pro Asp Phe 
                725                 730                 735     


Asn Thr Ile Asn Ser Thr Glu Glu Asn Gly Trp Lys Ala Ser Asn Gly 
            740                 745                 750         


Val Thr Ile Ser Glu Gly Gly Pro Phe Tyr Lys Gly Arg Ala Leu Gln 
        755                 760                 765             


Leu Ala Ser Ala Arg Glu Asn Tyr Pro Thr Tyr Ile Tyr Gln Lys Val 
    770                 775                 780                 


Asp Ala Ser Glu Leu Lys Pro Tyr Thr Arg Tyr Arg Leu Asp Gly Phe 
785                 790                 795                 800 


Val Lys Ser Ser Gln Asp Leu Glu Ile Asp Leu Ile His His His Lys 
                805                 810                 815     


Val His Leu Val Lys Asn Ala Pro Asp Asn Leu Val Ser Asp Thr Tyr 
            820                 825                 830         


Pro Asp Asp Ser Cys Ser Gly Ile Asn Arg Cys Gln Glu Gln Gln Met 
        835                 840                 845             


Val Asn Ala Gln Leu Glu Thr Glu His His His Pro Met Asp Cys Cys 
    850                 855                 860                 


Gly Ala Ala Gln Thr His Glu Phe Ser Ser Tyr Ile Asp Thr Gly Asp 
865                 870                 875                 880 


Leu Asn Ser Thr Val Asp Gln Gly Ile Trp Ala Ile Phe Lys Val Arg 
                885                 890                 895     


Thr Thr Asp Gly Tyr Ala Thr Leu Gly Asn Leu Glu Leu Val Glu Ile 
            900                 905                 910         


Gly Pro Leu Ser Gly Glu Ser Leu Glu Arg Glu Gln Arg Asp Asn Ala 
        915                 920                 925             


Lys Trp Ser Ala Glu Leu Gly Arg Lys Arg Ala Glu Thr Asp Arg Val 
    930                 935                 940                 


Tyr Gln Asp Ala Lys Gln Ser Ile Asn His Leu Phe Val Asp Tyr Gln 
945                 950                 955                 960 


Asp Gln Gln Leu Asn Pro Glu Ile Gly Met Ala Asp Ile Met Asp Ala 
                965                 970                 975     


Gln Asn Leu Val Ala Ser Ile Ser Asp Val Tyr Ser Asp Ala Val Leu 
            980                 985                 990         


Gln Ile Pro Gly Ile Asn Tyr Glu  Ile Tyr Thr Glu Leu  Ser Asn Arg 
        995                 1000                 1005             


Leu Gln  Gln Ala Ser Tyr Leu  Tyr Thr Ser Arg Asn  Ala Val Gln 
    1010                 1015                 1020             


Asn Gly  Asp Phe Asn Ser Gly  Leu Asp Ser Trp Asn  Ala Thr Ala 
    1025                 1030                 1035             


Gly Ala  Thr Val Gln Gln Asp  Gly Asn Thr His Phe  Leu Val Leu 
    1040                 1045                 1050             


Ser His  Trp Asp Ala Gln Val  Ser Gln Gln Phe Arg  Val Gln Pro 
    1055                 1060                 1065             


Asn Cys  Lys Tyr Val Leu Arg  Val Thr Ala Glu Lys  Val Gly Gly 
    1070                 1075                 1080             


Gly Asp  Gly Tyr Val Thr Ile  Arg Asp Gly Ala His  His Thr Glu 
    1085                 1090                 1095             


Thr Leu  Thr Phe Asn Ala Cys  Asp Tyr Asp Ile Asn  Gly Thr Tyr 
    1100                 1105                 1110             


Val Thr  Asp Asn Thr Tyr Leu  Thr Lys Glu Val Val  Phe Tyr Ser 
    1115                 1120                 1125             


His Thr  Glu His Met Trp Val  Glu Val Ser Glu Thr  Glu Gly Val 
    1130                 1135                 1140             


Phe His  Ile Asp Ser Val Glu  Phe Met Glu Thr Gln  Gln 
    1145                 1150                 1155     


<210>  37
<211>  3471
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic bacterial coding sequence encoding TIC4064_22 
       comprising mutations at S33G and S94T.

<400>  37
atgaatcaaa ataaacacgg aattattggc gcttccaatt gtggttgtac gtcagataat       60

gttgcgaaat atcctttagc caacaatcca tattcaggag ctttaaattt aaattcttgt      120

caaaatagta gtattctcaa ctggattaac ataataggcg atgcagcaaa agaagcagta      180

tctattggga caacaatagt ctctcttatc acagcacctt ctcttactgg attaatttca      240

atagtatatg accttatagg taaagtacta ggaggtagta caggacaatc catatcagat      300

ttgtctatat gtgacttatt atctattatt gatttacggg taagtcagag tgttttaaat      360

gatgggattg cagattttaa tggttctgta ctcttataca ggaactattt agaggctctg      420

gatagctgga ataagaatcc taattctgct tctgctgaag aactccgtac tcgttttaga      480

atcgctgact cagaatttga tagaatttta acacgagggt ctttaacgaa tggtggctcg      540

ttagctagac aaaatgccca aatattatta ttaccttctt ttgcgagtgc tgcatttttc      600

catttattac tactaaggga tgctactaga tatggcacta attgggggct atacaatgct      660

acacctttta taaattatca atcaaaacta gtagagctta ttgaactata tactgattat      720

tgcgtacatt ggtataatcg aggtttcaac gaactaaggc aacgaggcac tagtgctaca      780

gcttggttag aatttcatag atatcgtaga gagatgacat tgatggtatt agatatagta      840

gcatcatttt caagtcttga tattactaat tacccaatag aaacagattt tcagttgagt      900

agggtcattt atacagatcc aattggtttt gtacatcgta gtagtcttag gggagaaagt      960

tggtttagct ttgttaatag agctaatttc tcagatttag aaaatgcaat acctaatcct     1020

agaccgtctt ggtttttaaa taatatgatt atatctactg gttcacttac attgccggtt     1080

agcccaagta ctgatagagc gagggtatgg tatggaagtc gagatcgaat ttcccctgct     1140

aattcacaat ttattactga actaatctct ggacaacata cgactgctac acaaactatt     1200

ttagggcgaa atatatttag agtagattct caagcttgta atttaaatga taccacatat     1260

ggagtgaata gggcggtatt ttatcatgat gcgagtgaag gttctcaaag atccgtgtac     1320

gaggggtata ttcgaacaac tgggatagat aaccctagag ttcaaaatat taacacttat     1380

ttacctggag aaaattcaga tatcccaact ccagaagact atactcatat attaagcaca     1440

acaataaatt taacaggagg acttagacaa gtagcatcta atcgccgttc atctttagta     1500

atgtatggtt ggacacataa aagtctggct cgtaacaata ccattaatcc agatagaatt     1560

acacagatac cattgacgaa ggttgatacc cgaggcacag gtgtttctta tgtgaatgat     1620

ccaggattta taggaggagc tctacttcaa aggactgacc atggttcgct tggagtattg     1680

agggtccaat ttccacttca cttaagacaa caatatcgca ttagagtccg ttatgcttct     1740

acaacaaata ttcgattgag tgtgaatggc agtttcggta ctatttctca aaatctccct     1800

agtacaatga gattaggaga ggatttaaga tacggatctt ttgctataag agagtttagt     1860

acttctatta gacccactgc aagtccggac caaattcgat tgacaataga accatctttt     1920

attagacaag aggtctatgt agatagaatt gagttcattc cagttaatcc gacgcgagag     1980

gcgaaagagg atctagaagc aacaaagaaa gcggtggcga gcttgtttac acgcacaagg     2040

gacggattac aagtaaatgt gacagattat caagtcgatc aagcggcaaa tttagtgtca     2100

tgcttatcag atgaacaata tgcgcatgat aaaaagatgt tattggaagc ggtacgcgcg     2160

gcaaaacgcc tcagccgaga acgcaactta cttcaggatc cagattttaa tacaatcaat     2220

agtacagaag aaaatggatg gaaagcaagt aacggcgtta ctattagcga aggcggtcca     2280

ttctataaag gccgtgcact tcagctagca agtgcacgag aaaattaccc aacatacatc     2340

tatcaaaaag tagatgcatc ggagttaaag ccgtatacac gttatagact ggatgggttc     2400

gtgaagagta gtcaagattt agaaattgat ctcattcacc atcataaagt ccatcttgtg     2460

aaaaatgcac cagataattt agtatctgat acttacccag atgattcttg tagtggaatc     2520

aatcgatgtc aggaacaaca gatggtaaat gcgcaactgg aaacagaaca tcatcatccg     2580

atggattgct gtggagcagc tcaaacacat gagttttctt cctatattga tacaggggat     2640

ttaaattcga ctgtagacca gggaatctgg gcgatcttta aagttcgaac aacagatggt     2700

tatgcgacgt taggaaatct tgaattggta gagatcggac cgttatcggg tgaatctcta     2760

gaacgtgaac aaagggataa tgcaaaatgg agtgcagagc taggaagaaa gcgtgcagaa     2820

acagatcgcg tgtatcaaga tgccaaacaa tccatcaatc atttatttgt ggattatcaa     2880

gatcaacaat taaatccaga aatagggatg gcagatatta tggacgctca aaatcttgtc     2940

gcatcaattt cagatgtata tagcgatgca gtactgcaaa tccctggaat taactatgag     3000

atttacacag agctatccaa tcgcttacaa caagcatcgt atctgtatac gtctcgaaat     3060

gcggtgcaaa atggggactt taacagcggt ctagatagtt ggaatgcaac agcgggtgct     3120

acggtacaac aggatggtaa tacgcatttc ttagttcttt ctcattggga tgcacaagtt     3180

tctcaacaat ttagagtgca gccaaattgt aaatatgtat tacgtgtaac agcagagaaa     3240

gtaggcggcg gagacggata cgtgacaatc cgggatggtg ctcatcatac agaaacgctt     3300

acatttaatg catgtgatta tgatataaat ggcacgtacg tgactgataa tacgtatcta     3360

acaaaagaag tggtattcta ttcacataca gaacacatgt gggtagaggt aagtgaaaca     3420

gaaggtgttt tccatataga cagtgttgag ttcatggaaa cccaacagta g              3471


<210>  38
<211>  1156
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of TIC4064_22 comprising mutations at S33G 
       and S94T.

<400>  38

Met Asn Gln Asn Lys His Gly Ile Ile Gly Ala Ser Asn Cys Gly Cys 
1               5                   10                  15      


Thr Ser Asp Asn Val Ala Lys Tyr Pro Leu Ala Asn Asn Pro Tyr Ser 
            20                  25                  30          


Gly Ala Leu Asn Leu Asn Ser Cys Gln Asn Ser Ser Ile Leu Asn Trp 
        35                  40                  45              


Ile Asn Ile Ile Gly Asp Ala Ala Lys Glu Ala Val Ser Ile Gly Thr 
    50                  55                  60                  


Thr Ile Val Ser Leu Ile Thr Ala Pro Ser Leu Thr Gly Leu Ile Ser 
65                  70                  75                  80  


Ile Val Tyr Asp Leu Ile Gly Lys Val Leu Gly Gly Ser Thr Gly Gln 
                85                  90                  95      


Ser Ile Ser Asp Leu Ser Ile Cys Asp Leu Leu Ser Ile Ile Asp Leu 
            100                 105                 110         


Arg Val Ser Gln Ser Val Leu Asn Asp Gly Ile Ala Asp Phe Asn Gly 
        115                 120                 125             


Ser Val Leu Leu Tyr Arg Asn Tyr Leu Glu Ala Leu Asp Ser Trp Asn 
    130                 135                 140                 


Lys Asn Pro Asn Ser Ala Ser Ala Glu Glu Leu Arg Thr Arg Phe Arg 
145                 150                 155                 160 


Ile Ala Asp Ser Glu Phe Asp Arg Ile Leu Thr Arg Gly Ser Leu Thr 
                165                 170                 175     


Asn Gly Gly Ser Leu Ala Arg Gln Asn Ala Gln Ile Leu Leu Leu Pro 
            180                 185                 190         


Ser Phe Ala Ser Ala Ala Phe Phe His Leu Leu Leu Leu Arg Asp Ala 
        195                 200                 205             


Thr Arg Tyr Gly Thr Asn Trp Gly Leu Tyr Asn Ala Thr Pro Phe Ile 
    210                 215                 220                 


Asn Tyr Gln Ser Lys Leu Val Glu Leu Ile Glu Leu Tyr Thr Asp Tyr 
225                 230                 235                 240 


Cys Val His Trp Tyr Asn Arg Gly Phe Asn Glu Leu Arg Gln Arg Gly 
                245                 250                 255     


Thr Ser Ala Thr Ala Trp Leu Glu Phe His Arg Tyr Arg Arg Glu Met 
            260                 265                 270         


Thr Leu Met Val Leu Asp Ile Val Ala Ser Phe Ser Ser Leu Asp Ile 
        275                 280                 285             


Thr Asn Tyr Pro Ile Glu Thr Asp Phe Gln Leu Ser Arg Val Ile Tyr 
    290                 295                 300                 


Thr Asp Pro Ile Gly Phe Val His Arg Ser Ser Leu Arg Gly Glu Ser 
305                 310                 315                 320 


Trp Phe Ser Phe Val Asn Arg Ala Asn Phe Ser Asp Leu Glu Asn Ala 
                325                 330                 335     


Ile Pro Asn Pro Arg Pro Ser Trp Phe Leu Asn Asn Met Ile Ile Ser 
            340                 345                 350         


Thr Gly Ser Leu Thr Leu Pro Val Ser Pro Ser Thr Asp Arg Ala Arg 
        355                 360                 365             


Val Trp Tyr Gly Ser Arg Asp Arg Ile Ser Pro Ala Asn Ser Gln Phe 
    370                 375                 380                 


Ile Thr Glu Leu Ile Ser Gly Gln His Thr Thr Ala Thr Gln Thr Ile 
385                 390                 395                 400 


Leu Gly Arg Asn Ile Phe Arg Val Asp Ser Gln Ala Cys Asn Leu Asn 
                405                 410                 415     


Asp Thr Thr Tyr Gly Val Asn Arg Ala Val Phe Tyr His Asp Ala Ser 
            420                 425                 430         


Glu Gly Ser Gln Arg Ser Val Tyr Glu Gly Tyr Ile Arg Thr Thr Gly 
        435                 440                 445             


Ile Asp Asn Pro Arg Val Gln Asn Ile Asn Thr Tyr Leu Pro Gly Glu 
    450                 455                 460                 


Asn Ser Asp Ile Pro Thr Pro Glu Asp Tyr Thr His Ile Leu Ser Thr 
465                 470                 475                 480 


Thr Ile Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Ser Asn Arg Arg 
                485                 490                 495     


Ser Ser Leu Val Met Tyr Gly Trp Thr His Lys Ser Leu Ala Arg Asn 
            500                 505                 510         


Asn Thr Ile Asn Pro Asp Arg Ile Thr Gln Ile Pro Leu Thr Lys Val 
        515                 520                 525             


Asp Thr Arg Gly Thr Gly Val Ser Tyr Val Asn Asp Pro Gly Phe Ile 
    530                 535                 540                 


Gly Gly Ala Leu Leu Gln Arg Thr Asp His Gly Ser Leu Gly Val Leu 
545                 550                 555                 560 


Arg Val Gln Phe Pro Leu His Leu Arg Gln Gln Tyr Arg Ile Arg Val 
                565                 570                 575     


Arg Tyr Ala Ser Thr Thr Asn Ile Arg Leu Ser Val Asn Gly Ser Phe 
            580                 585                 590         


Gly Thr Ile Ser Gln Asn Leu Pro Ser Thr Met Arg Leu Gly Glu Asp 
        595                 600                 605             


Leu Arg Tyr Gly Ser Phe Ala Ile Arg Glu Phe Ser Thr Ser Ile Arg 
    610                 615                 620                 


Pro Thr Ala Ser Pro Asp Gln Ile Arg Leu Thr Ile Glu Pro Ser Phe 
625                 630                 635                 640 


Ile Arg Gln Glu Val Tyr Val Asp Arg Ile Glu Phe Ile Pro Val Asn 
                645                 650                 655     


Pro Thr Arg Glu Ala Lys Glu Asp Leu Glu Ala Thr Lys Lys Ala Val 
            660                 665                 670         


Ala Ser Leu Phe Thr Arg Thr Arg Asp Gly Leu Gln Val Asn Val Thr 
        675                 680                 685             


Asp Tyr Gln Val Asp Gln Ala Ala Asn Leu Val Ser Cys Leu Ser Asp 
    690                 695                 700                 


Glu Gln Tyr Ala His Asp Lys Lys Met Leu Leu Glu Ala Val Arg Ala 
705                 710                 715                 720 


Ala Lys Arg Leu Ser Arg Glu Arg Asn Leu Leu Gln Asp Pro Asp Phe 
                725                 730                 735     


Asn Thr Ile Asn Ser Thr Glu Glu Asn Gly Trp Lys Ala Ser Asn Gly 
            740                 745                 750         


Val Thr Ile Ser Glu Gly Gly Pro Phe Tyr Lys Gly Arg Ala Leu Gln 
        755                 760                 765             


Leu Ala Ser Ala Arg Glu Asn Tyr Pro Thr Tyr Ile Tyr Gln Lys Val 
    770                 775                 780                 


Asp Ala Ser Glu Leu Lys Pro Tyr Thr Arg Tyr Arg Leu Asp Gly Phe 
785                 790                 795                 800 


Val Lys Ser Ser Gln Asp Leu Glu Ile Asp Leu Ile His His His Lys 
                805                 810                 815     


Val His Leu Val Lys Asn Ala Pro Asp Asn Leu Val Ser Asp Thr Tyr 
            820                 825                 830         


Pro Asp Asp Ser Cys Ser Gly Ile Asn Arg Cys Gln Glu Gln Gln Met 
        835                 840                 845             


Val Asn Ala Gln Leu Glu Thr Glu His His His Pro Met Asp Cys Cys 
    850                 855                 860                 


Gly Ala Ala Gln Thr His Glu Phe Ser Ser Tyr Ile Asp Thr Gly Asp 
865                 870                 875                 880 


Leu Asn Ser Thr Val Asp Gln Gly Ile Trp Ala Ile Phe Lys Val Arg 
                885                 890                 895     


Thr Thr Asp Gly Tyr Ala Thr Leu Gly Asn Leu Glu Leu Val Glu Ile 
            900                 905                 910         


Gly Pro Leu Ser Gly Glu Ser Leu Glu Arg Glu Gln Arg Asp Asn Ala 
        915                 920                 925             


Lys Trp Ser Ala Glu Leu Gly Arg Lys Arg Ala Glu Thr Asp Arg Val 
    930                 935                 940                 


Tyr Gln Asp Ala Lys Gln Ser Ile Asn His Leu Phe Val Asp Tyr Gln 
945                 950                 955                 960 


Asp Gln Gln Leu Asn Pro Glu Ile Gly Met Ala Asp Ile Met Asp Ala 
                965                 970                 975     


Gln Asn Leu Val Ala Ser Ile Ser Asp Val Tyr Ser Asp Ala Val Leu 
            980                 985                 990         


Gln Ile Pro Gly Ile Asn Tyr Glu  Ile Tyr Thr Glu Leu  Ser Asn Arg 
        995                 1000                 1005             


Leu Gln  Gln Ala Ser Tyr Leu  Tyr Thr Ser Arg Asn  Ala Val Gln 
    1010                 1015                 1020             


Asn Gly  Asp Phe Asn Ser Gly  Leu Asp Ser Trp Asn  Ala Thr Ala 
    1025                 1030                 1035             


Gly Ala  Thr Val Gln Gln Asp  Gly Asn Thr His Phe  Leu Val Leu 
    1040                 1045                 1050             


Ser His  Trp Asp Ala Gln Val  Ser Gln Gln Phe Arg  Val Gln Pro 
    1055                 1060                 1065             


Asn Cys  Lys Tyr Val Leu Arg  Val Thr Ala Glu Lys  Val Gly Gly 
    1070                 1075                 1080             


Gly Asp  Gly Tyr Val Thr Ile  Arg Asp Gly Ala His  His Thr Glu 
    1085                 1090                 1095             


Thr Leu  Thr Phe Asn Ala Cys  Asp Tyr Asp Ile Asn  Gly Thr Tyr 
    1100                 1105                 1110             


Val Thr  Asp Asn Thr Tyr Leu  Thr Lys Glu Val Val  Phe Tyr Ser 
    1115                 1120                 1125             


His Thr  Glu His Met Trp Val  Glu Val Ser Glu Thr  Glu Gly Val 
    1130                 1135                 1140             


Phe His  Ile Asp Ser Val Glu  Phe Met Glu Thr Gln  Gln 
    1145                 1150                 1155     


<210>  39
<211>  3471
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic bacterial coding sequence encoding TIC4064_23 
       comprising mutations at S94T, E153D, Q436I, and S596Q.

<400>  39
atgaatcaaa ataaacacgg aattattggc gcttccaatt gtggttgtac gtcagataat       60

gttgcgaaat atcctttagc caacaatcca tattcatctg ctttaaattt aaattcttgt      120

caaaatagta gtattctcaa ctggattaac ataataggcg atgcagcaaa agaagcagta      180

tctattggga caacaatagt ctctcttatc acagcacctt ctcttactgg attaatttca      240

atagtatatg accttatagg taaagtacta ggaggtagta caggacaatc catatcagat      300

ttgtctatat gtgacttatt atctattatt gatttacggg taagtcagag tgttttaaat      360

gatgggattg cagattttaa tggttctgta ctcttataca ggaactattt agaggctctg      420

gatagctgga ataagaatcc taattctgct tctgctgatg aactccgtac tcgttttaga      480

atcgctgact cagaatttga tagaatttta acacgagggt ctttaacgaa tggtggctcg      540

ttagctagac aaaatgccca aatattatta ttaccttctt ttgcgagtgc tgcatttttc      600

catttattac tactaaggga tgctactaga tatggcacta attgggggct atacaatgct      660

acacctttta taaattatca atcaaaacta gtagagctta ttgaactata tactgattat      720

tgcgtacatt ggtataatcg aggtttcaac gaactaaggc aacgaggcac tagtgctaca      780

gcttggttag aatttcatag atatcgtaga gagatgacat tgatggtatt agatatagta      840

gcatcatttt caagtcttga tattactaat tacccaatag aaacagattt tcagttgagt      900

agggtcattt atacagatcc aattggtttt gtacatcgta gtagtcttag gggagaaagt      960

tggtttagct ttgttaatag agctaatttc tcagatttag aaaatgcaat acctaatcct     1020

agaccgtctt ggtttttaaa taatatgatt atatctactg gttcacttac attgccggtt     1080

agcccaagta ctgatagagc gagggtatgg tatggaagtc gagatcgaat ttcccctgct     1140

aattcacaat ttattactga actaatctct ggacaacata cgactgctac acaaactatt     1200

ttagggcgaa atatatttag agtagattct caagcttgta atttaaatga taccacatat     1260

ggagtgaata gggcggtatt ttatcatgat gcgagtgaag gttctattag atccgtgtac     1320

gaggggtata ttcgaacaac tgggatagat aaccctagag ttcaaaatat taacacttat     1380

ttacctggag aaaattcaga tatcccaact ccagaagact atactcatat attaagcaca     1440

acaataaatt taacaggagg acttagacaa gtagcatcta atcgccgttc atctttagta     1500

atgtatggtt ggacacataa aagtctggct cgtaacaata ccattaatcc agatagaatt     1560

acacagatac cattgacgaa ggttgatacc cgaggcacag gtgtttctta tgtgaatgat     1620

ccaggattta taggaggagc tctacttcaa aggactgacc atggttcgct tggagtattg     1680

agggtccaat ttccacttca cttaagacaa caatatcgca ttagagtccg ttatgcttct     1740

acaacaaata ttcgattgag tgtgaatggc agtttcggta ctattcaaca aaatctccct     1800

agtacaatga gattaggaga ggatttaaga tacggatctt ttgctataag agagtttagt     1860

acttctatta gacccactgc aagtccggac caaattcgat tgacaataga accatctttt     1920

attagacaag aggtctatgt agatagaatt gagttcattc cagttaatcc gacgcgagag     1980

gcgaaagagg atctagaagc aacaaagaaa gcggtggcga gcttgtttac acgcacaagg     2040

gacggattac aagtaaatgt gacagattat caagtcgatc aagcggcaaa tttagtgtca     2100

tgcttatcag atgaacaata tgcgcatgat aaaaagatgt tattggaagc ggtacgcgcg     2160

gcaaaacgcc tcagccgaga acgcaactta cttcaggatc cagattttaa tacaatcaat     2220

agtacagaag aaaatggatg gaaagcaagt aacggcgtta ctattagcga aggcggtcca     2280

ttctataaag gccgtgcact tcagctagca agtgcacgag aaaattaccc aacatacatc     2340

tatcaaaaag tagatgcatc ggagttaaag ccgtatacac gttatagact ggatgggttc     2400

gtgaagagta gtcaagattt agaaattgat ctcattcacc atcataaagt ccatcttgtg     2460

aaaaatgcac cagataattt agtatctgat acttacccag atgattcttg tagtggaatc     2520

aatcgatgtc aggaacaaca gatggtaaat gcgcaactgg aaacagaaca tcatcatccg     2580

atggattgct gtggagcagc tcaaacacat gagttttctt cctatattga tacaggggat     2640

ttaaattcga ctgtagacca gggaatctgg gcgatcttta aagttcgaac aacagatggt     2700

tatgcgacgt taggaaatct tgaattggta gagatcggac cgttatcggg tgaatctcta     2760

gaacgtgaac aaagggataa tgcaaaatgg agtgcagagc taggaagaaa gcgtgcagaa     2820

acagatcgcg tgtatcaaga tgccaaacaa tccatcaatc atttatttgt ggattatcaa     2880

gatcaacaat taaatccaga aatagggatg gcagatatta tggacgctca aaatcttgtc     2940

gcatcaattt cagatgtata tagcgatgca gtactgcaaa tccctggaat taactatgag     3000

atttacacag agctatccaa tcgcttacaa caagcatcgt atctgtatac gtctcgaaat     3060

gcggtgcaaa atggggactt taacagcggt ctagatagtt ggaatgcaac agcgggtgct     3120

acggtacaac aggatggtaa tacgcatttc ttagttcttt ctcattggga tgcacaagtt     3180

tctcaacaat ttagagtgca gccaaattgt aaatatgtat tacgtgtaac agcagagaaa     3240

gtaggcggcg gagacggata cgtgacaatc cgggatggtg ctcatcatac agaaacgctt     3300

acatttaatg catgtgatta tgatataaat ggcacgtacg tgactgataa tacgtatcta     3360

acaaaagaag tggtattcta ttcacataca gaacacatgt gggtagaggt aagtgaaaca     3420

gaaggtgttt tccatataga cagtgttgag ttcatggaaa cccaacagta g              3471


<210>  40
<211>  1156
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of TIC4064_23 comprising mutations at S94T, 
       E153D, Q436I, and S596Q.

<400>  40

Met Asn Gln Asn Lys His Gly Ile Ile Gly Ala Ser Asn Cys Gly Cys 
1               5                   10                  15      


Thr Ser Asp Asn Val Ala Lys Tyr Pro Leu Ala Asn Asn Pro Tyr Ser 
            20                  25                  30          


Ser Ala Leu Asn Leu Asn Ser Cys Gln Asn Ser Ser Ile Leu Asn Trp 
        35                  40                  45              


Ile Asn Ile Ile Gly Asp Ala Ala Lys Glu Ala Val Ser Ile Gly Thr 
    50                  55                  60                  


Thr Ile Val Ser Leu Ile Thr Ala Pro Ser Leu Thr Gly Leu Ile Ser 
65                  70                  75                  80  


Ile Val Tyr Asp Leu Ile Gly Lys Val Leu Gly Gly Ser Thr Gly Gln 
                85                  90                  95      


Ser Ile Ser Asp Leu Ser Ile Cys Asp Leu Leu Ser Ile Ile Asp Leu 
            100                 105                 110         


Arg Val Ser Gln Ser Val Leu Asn Asp Gly Ile Ala Asp Phe Asn Gly 
        115                 120                 125             


Ser Val Leu Leu Tyr Arg Asn Tyr Leu Glu Ala Leu Asp Ser Trp Asn 
    130                 135                 140                 


Lys Asn Pro Asn Ser Ala Ser Ala Asp Glu Leu Arg Thr Arg Phe Arg 
145                 150                 155                 160 


Ile Ala Asp Ser Glu Phe Asp Arg Ile Leu Thr Arg Gly Ser Leu Thr 
                165                 170                 175     


Asn Gly Gly Ser Leu Ala Arg Gln Asn Ala Gln Ile Leu Leu Leu Pro 
            180                 185                 190         


Ser Phe Ala Ser Ala Ala Phe Phe His Leu Leu Leu Leu Arg Asp Ala 
        195                 200                 205             


Thr Arg Tyr Gly Thr Asn Trp Gly Leu Tyr Asn Ala Thr Pro Phe Ile 
    210                 215                 220                 


Asn Tyr Gln Ser Lys Leu Val Glu Leu Ile Glu Leu Tyr Thr Asp Tyr 
225                 230                 235                 240 


Cys Val His Trp Tyr Asn Arg Gly Phe Asn Glu Leu Arg Gln Arg Gly 
                245                 250                 255     


Thr Ser Ala Thr Ala Trp Leu Glu Phe His Arg Tyr Arg Arg Glu Met 
            260                 265                 270         


Thr Leu Met Val Leu Asp Ile Val Ala Ser Phe Ser Ser Leu Asp Ile 
        275                 280                 285             


Thr Asn Tyr Pro Ile Glu Thr Asp Phe Gln Leu Ser Arg Val Ile Tyr 
    290                 295                 300                 


Thr Asp Pro Ile Gly Phe Val His Arg Ser Ser Leu Arg Gly Glu Ser 
305                 310                 315                 320 


Trp Phe Ser Phe Val Asn Arg Ala Asn Phe Ser Asp Leu Glu Asn Ala 
                325                 330                 335     


Ile Pro Asn Pro Arg Pro Ser Trp Phe Leu Asn Asn Met Ile Ile Ser 
            340                 345                 350         


Thr Gly Ser Leu Thr Leu Pro Val Ser Pro Ser Thr Asp Arg Ala Arg 
        355                 360                 365             


Val Trp Tyr Gly Ser Arg Asp Arg Ile Ser Pro Ala Asn Ser Gln Phe 
    370                 375                 380                 


Ile Thr Glu Leu Ile Ser Gly Gln His Thr Thr Ala Thr Gln Thr Ile 
385                 390                 395                 400 


Leu Gly Arg Asn Ile Phe Arg Val Asp Ser Gln Ala Cys Asn Leu Asn 
                405                 410                 415     


Asp Thr Thr Tyr Gly Val Asn Arg Ala Val Phe Tyr His Asp Ala Ser 
            420                 425                 430         


Glu Gly Ser Ile Arg Ser Val Tyr Glu Gly Tyr Ile Arg Thr Thr Gly 
        435                 440                 445             


Ile Asp Asn Pro Arg Val Gln Asn Ile Asn Thr Tyr Leu Pro Gly Glu 
    450                 455                 460                 


Asn Ser Asp Ile Pro Thr Pro Glu Asp Tyr Thr His Ile Leu Ser Thr 
465                 470                 475                 480 


Thr Ile Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Ser Asn Arg Arg 
                485                 490                 495     


Ser Ser Leu Val Met Tyr Gly Trp Thr His Lys Ser Leu Ala Arg Asn 
            500                 505                 510         


Asn Thr Ile Asn Pro Asp Arg Ile Thr Gln Ile Pro Leu Thr Lys Val 
        515                 520                 525             


Asp Thr Arg Gly Thr Gly Val Ser Tyr Val Asn Asp Pro Gly Phe Ile 
    530                 535                 540                 


Gly Gly Ala Leu Leu Gln Arg Thr Asp His Gly Ser Leu Gly Val Leu 
545                 550                 555                 560 


Arg Val Gln Phe Pro Leu His Leu Arg Gln Gln Tyr Arg Ile Arg Val 
                565                 570                 575     


Arg Tyr Ala Ser Thr Thr Asn Ile Arg Leu Ser Val Asn Gly Ser Phe 
            580                 585                 590         


Gly Thr Ile Gln Gln Asn Leu Pro Ser Thr Met Arg Leu Gly Glu Asp 
        595                 600                 605             


Leu Arg Tyr Gly Ser Phe Ala Ile Arg Glu Phe Ser Thr Ser Ile Arg 
    610                 615                 620                 


Pro Thr Ala Ser Pro Asp Gln Ile Arg Leu Thr Ile Glu Pro Ser Phe 
625                 630                 635                 640 


Ile Arg Gln Glu Val Tyr Val Asp Arg Ile Glu Phe Ile Pro Val Asn 
                645                 650                 655     


Pro Thr Arg Glu Ala Lys Glu Asp Leu Glu Ala Thr Lys Lys Ala Val 
            660                 665                 670         


Ala Ser Leu Phe Thr Arg Thr Arg Asp Gly Leu Gln Val Asn Val Thr 
        675                 680                 685             


Asp Tyr Gln Val Asp Gln Ala Ala Asn Leu Val Ser Cys Leu Ser Asp 
    690                 695                 700                 


Glu Gln Tyr Ala His Asp Lys Lys Met Leu Leu Glu Ala Val Arg Ala 
705                 710                 715                 720 


Ala Lys Arg Leu Ser Arg Glu Arg Asn Leu Leu Gln Asp Pro Asp Phe 
                725                 730                 735     


Asn Thr Ile Asn Ser Thr Glu Glu Asn Gly Trp Lys Ala Ser Asn Gly 
            740                 745                 750         


Val Thr Ile Ser Glu Gly Gly Pro Phe Tyr Lys Gly Arg Ala Leu Gln 
        755                 760                 765             


Leu Ala Ser Ala Arg Glu Asn Tyr Pro Thr Tyr Ile Tyr Gln Lys Val 
    770                 775                 780                 


Asp Ala Ser Glu Leu Lys Pro Tyr Thr Arg Tyr Arg Leu Asp Gly Phe 
785                 790                 795                 800 


Val Lys Ser Ser Gln Asp Leu Glu Ile Asp Leu Ile His His His Lys 
                805                 810                 815     


Val His Leu Val Lys Asn Ala Pro Asp Asn Leu Val Ser Asp Thr Tyr 
            820                 825                 830         


Pro Asp Asp Ser Cys Ser Gly Ile Asn Arg Cys Gln Glu Gln Gln Met 
        835                 840                 845             


Val Asn Ala Gln Leu Glu Thr Glu His His His Pro Met Asp Cys Cys 
    850                 855                 860                 


Gly Ala Ala Gln Thr His Glu Phe Ser Ser Tyr Ile Asp Thr Gly Asp 
865                 870                 875                 880 


Leu Asn Ser Thr Val Asp Gln Gly Ile Trp Ala Ile Phe Lys Val Arg 
                885                 890                 895     


Thr Thr Asp Gly Tyr Ala Thr Leu Gly Asn Leu Glu Leu Val Glu Ile 
            900                 905                 910         


Gly Pro Leu Ser Gly Glu Ser Leu Glu Arg Glu Gln Arg Asp Asn Ala 
        915                 920                 925             


Lys Trp Ser Ala Glu Leu Gly Arg Lys Arg Ala Glu Thr Asp Arg Val 
    930                 935                 940                 


Tyr Gln Asp Ala Lys Gln Ser Ile Asn His Leu Phe Val Asp Tyr Gln 
945                 950                 955                 960 


Asp Gln Gln Leu Asn Pro Glu Ile Gly Met Ala Asp Ile Met Asp Ala 
                965                 970                 975     


Gln Asn Leu Val Ala Ser Ile Ser Asp Val Tyr Ser Asp Ala Val Leu 
            980                 985                 990         


Gln Ile Pro Gly Ile Asn Tyr Glu  Ile Tyr Thr Glu Leu  Ser Asn Arg 
        995                 1000                 1005             


Leu Gln  Gln Ala Ser Tyr Leu  Tyr Thr Ser Arg Asn  Ala Val Gln 
    1010                 1015                 1020             


Asn Gly  Asp Phe Asn Ser Gly  Leu Asp Ser Trp Asn  Ala Thr Ala 
    1025                 1030                 1035             


Gly Ala  Thr Val Gln Gln Asp  Gly Asn Thr His Phe  Leu Val Leu 
    1040                 1045                 1050             


Ser His  Trp Asp Ala Gln Val  Ser Gln Gln Phe Arg  Val Gln Pro 
    1055                 1060                 1065             


Asn Cys  Lys Tyr Val Leu Arg  Val Thr Ala Glu Lys  Val Gly Gly 
    1070                 1075                 1080             


Gly Asp  Gly Tyr Val Thr Ile  Arg Asp Gly Ala His  His Thr Glu 
    1085                 1090                 1095             


Thr Leu  Thr Phe Asn Ala Cys  Asp Tyr Asp Ile Asn  Gly Thr Tyr 
    1100                 1105                 1110             


Val Thr  Asp Asn Thr Tyr Leu  Thr Lys Glu Val Val  Phe Tyr Ser 
    1115                 1120                 1125             


His Thr  Glu His Met Trp Val  Glu Val Ser Glu Thr  Glu Gly Val 
    1130                 1135                 1140             


Phe His  Ile Asp Ser Val Glu  Phe Met Glu Thr Gln  Gln 
    1145                 1150                 1155     


<210>  41
<211>  3471
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic bacterial coding sequence encoding TIC4064_24 
       comprising mutations at G87K, W370L, H554N, and R585Q.

<400>  41
atgaatcaaa ataaacacgg aattattggc gcttccaatt gtggttgtac gtcagataat       60

gttgcgaaat atcctttagc caacaatcca tattcatctg ctttaaattt aaattcttgt      120

caaaatagta gtattctcaa ctggattaac ataataggcg atgcagcaaa agaagcagta      180

tctattggga caacaatagt ctctcttatc acagcacctt ctcttactgg attaatttca      240

atagtatatg accttataaa aaaagtacta ggaggtagta gtggacaatc catatcagat      300

ttgtctatat gtgacttatt atctattatt gatttacggg taagtcagag tgttttaaat      360

gatgggattg cagattttaa tggttctgta ctcttataca ggaactattt agaggctctg      420

gatagctgga ataagaatcc taattctgct tctgctgaag aactccgtac tcgttttaga      480

atcgctgact cagaatttga tagaatttta acacgagggt ctttaacgaa tggtggctcg      540

ttagctagac aaaatgccca aatattatta ttaccttctt ttgcgagtgc tgcatttttc      600

catttattac tactaaggga tgctactaga tatggcacta attgggggct atacaatgct      660

acacctttta taaattatca atcaaaacta gtagagctta ttgaactata tactgattat      720

tgcgtacatt ggtataatcg aggtttcaac gaactaaggc aacgaggcac tagtgctaca      780

gcttggttag aatttcatag atatcgtaga gagatgacat tgatggtatt agatatagta      840

gcatcatttt caagtcttga tattactaat tacccaatag aaacagattt tcagttgagt      900

agggtcattt atacagatcc aattggtttt gtacatcgta gtagtcttag gggagaaagt      960

tggtttagct ttgttaatag agctaatttc tcagatttag aaaatgcaat acctaatcct     1020

agaccgtctt ggtttttaaa taatatgatt atatctactg gttcacttac attgccggtt     1080

agcccaagta ctgatagagc gagggtatta tatggaagtc gagatcgaat ttcccctgct     1140

aattcacaat ttattactga actaatctct ggacaacata cgactgctac acaaactatt     1200

ttagggcgaa atatatttag agtagattct caagcttgta atttaaatga taccacatat     1260

ggagtgaata gggcggtatt ttatcatgat gcgagtgaag gttctcaaag atccgtgtac     1320

gaggggtata ttcgaacaac tgggatagat aaccctagag ttcaaaatat taacacttat     1380

ttacctggag aaaattcaga tatcccaact ccagaagact atactcatat attaagcaca     1440

acaataaatt taacaggagg acttagacaa gtagcatcta atcgccgttc atctttagta     1500

atgtatggtt ggacacataa aagtctggct cgtaacaata ccattaatcc agatagaatt     1560

acacagatac cattgacgaa ggttgatacc cgaggcacag gtgtttctta tgtgaatgat     1620

ccaggattta taggaggagc tctacttcaa aggactgaca atggttcgct tggagtattg     1680

agggtccaat ttccacttca cttaagacaa caatatcgca ttagagtccg ttatgcttct     1740

acaacaaata ttcaattgag tgtgaatggc agtttcggta ctatttctca aaatctccct     1800

agtacaatga gattaggaga ggatttaaga tacggatctt ttgctataag agagtttagt     1860

acttctatta gacccactgc aagtccggac caaattcgat tgacaataga accatctttt     1920

attagacaag aggtctatgt agatagaatt gagttcattc cagttaatcc gacgcgagag     1980

gcgaaagagg atctagaagc aacaaagaaa gcggtggcga gcttgtttac acgcacaagg     2040

gacggattac aagtaaatgt gacagattat caagtcgatc aagcggcaaa tttagtgtca     2100

tgcttatcag atgaacaata tgcgcatgat aaaaagatgt tattggaagc ggtacgcgcg     2160

gcaaaacgcc tcagccgaga acgcaactta cttcaggatc cagattttaa tacaatcaat     2220

agtacagaag aaaatggatg gaaagcaagt aacggcgtta ctattagcga aggcggtcca     2280

ttctataaag gccgtgcact tcagctagca agtgcacgag aaaattaccc aacatacatc     2340

tatcaaaaag tagatgcatc ggagttaaag ccgtatacac gttatagact ggatgggttc     2400

gtgaagagta gtcaagattt agaaattgat ctcattcacc atcataaagt ccatcttgtg     2460

aaaaatgcac cagataattt agtatctgat acttacccag atgattcttg tagtggaatc     2520

aatcgatgtc aggaacaaca gatggtaaat gcgcaactgg aaacagaaca tcatcatccg     2580

atggattgct gtggagcagc tcaaacacat gagttttctt cctatattga tacaggggat     2640

ttaaattcga ctgtagacca gggaatctgg gcgatcttta aagttcgaac aacagatggt     2700

tatgcgacgt taggaaatct tgaattggta gagatcggac cgttatcggg tgaatctcta     2760

gaacgtgaac aaagggataa tgcaaaatgg agtgcagagc taggaagaaa gcgtgcagaa     2820

acagatcgcg tgtatcaaga tgccaaacaa tccatcaatc atttatttgt ggattatcaa     2880

gatcaacaat taaatccaga aatagggatg gcagatatta tggacgctca aaatcttgtc     2940

gcatcaattt cagatgtata tagcgatgca gtactgcaaa tccctggaat taactatgag     3000

atttacacag agctatccaa tcgcttacaa caagcatcgt atctgtatac gtctcgaaat     3060

gcggtgcaaa atggggactt taacagcggt ctagatagtt ggaatgcaac agcgggtgct     3120

acggtacaac aggatggtaa tacgcatttc ttagttcttt ctcattggga tgcacaagtt     3180

tctcaacaat ttagagtgca gccaaattgt aaatatgtat tacgtgtaac agcagagaaa     3240

gtaggcggcg gagacggata cgtgacaatc cgggatggtg ctcatcatac agaaacgctt     3300

acatttaatg catgtgatta tgatataaat ggcacgtacg tgactgataa tacgtatcta     3360

acaaaagaag tggtattcta ttcacataca gaacacatgt gggtagaggt aagtgaaaca     3420

gaaggtgttt tccatataga cagtgttgag ttcatggaaa cccaacagta g              3471


<210>  42
<211>  1156
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of TIC4064_24 comprising mutations at G87K, 
       W370L, H554N, and R585Q.

<400>  42

Met Asn Gln Asn Lys His Gly Ile Ile Gly Ala Ser Asn Cys Gly Cys 
1               5                   10                  15      


Thr Ser Asp Asn Val Ala Lys Tyr Pro Leu Ala Asn Asn Pro Tyr Ser 
            20                  25                  30          


Ser Ala Leu Asn Leu Asn Ser Cys Gln Asn Ser Ser Ile Leu Asn Trp 
        35                  40                  45              


Ile Asn Ile Ile Gly Asp Ala Ala Lys Glu Ala Val Ser Ile Gly Thr 
    50                  55                  60                  


Thr Ile Val Ser Leu Ile Thr Ala Pro Ser Leu Thr Gly Leu Ile Ser 
65                  70                  75                  80  


Ile Val Tyr Asp Leu Ile Lys Lys Val Leu Gly Gly Ser Ser Gly Gln 
                85                  90                  95      


Ser Ile Ser Asp Leu Ser Ile Cys Asp Leu Leu Ser Ile Ile Asp Leu 
            100                 105                 110         


Arg Val Ser Gln Ser Val Leu Asn Asp Gly Ile Ala Asp Phe Asn Gly 
        115                 120                 125             


Ser Val Leu Leu Tyr Arg Asn Tyr Leu Glu Ala Leu Asp Ser Trp Asn 
    130                 135                 140                 


Lys Asn Pro Asn Ser Ala Ser Ala Glu Glu Leu Arg Thr Arg Phe Arg 
145                 150                 155                 160 


Ile Ala Asp Ser Glu Phe Asp Arg Ile Leu Thr Arg Gly Ser Leu Thr 
                165                 170                 175     


Asn Gly Gly Ser Leu Ala Arg Gln Asn Ala Gln Ile Leu Leu Leu Pro 
            180                 185                 190         


Ser Phe Ala Ser Ala Ala Phe Phe His Leu Leu Leu Leu Arg Asp Ala 
        195                 200                 205             


Thr Arg Tyr Gly Thr Asn Trp Gly Leu Tyr Asn Ala Thr Pro Phe Ile 
    210                 215                 220                 


Asn Tyr Gln Ser Lys Leu Val Glu Leu Ile Glu Leu Tyr Thr Asp Tyr 
225                 230                 235                 240 


Cys Val His Trp Tyr Asn Arg Gly Phe Asn Glu Leu Arg Gln Arg Gly 
                245                 250                 255     


Thr Ser Ala Thr Ala Trp Leu Glu Phe His Arg Tyr Arg Arg Glu Met 
            260                 265                 270         


Thr Leu Met Val Leu Asp Ile Val Ala Ser Phe Ser Ser Leu Asp Ile 
        275                 280                 285             


Thr Asn Tyr Pro Ile Glu Thr Asp Phe Gln Leu Ser Arg Val Ile Tyr 
    290                 295                 300                 


Thr Asp Pro Ile Gly Phe Val His Arg Ser Ser Leu Arg Gly Glu Ser 
305                 310                 315                 320 


Trp Phe Ser Phe Val Asn Arg Ala Asn Phe Ser Asp Leu Glu Asn Ala 
                325                 330                 335     


Ile Pro Asn Pro Arg Pro Ser Trp Phe Leu Asn Asn Met Ile Ile Ser 
            340                 345                 350         


Thr Gly Ser Leu Thr Leu Pro Val Ser Pro Ser Thr Asp Arg Ala Arg 
        355                 360                 365             


Val Leu Tyr Gly Ser Arg Asp Arg Ile Ser Pro Ala Asn Ser Gln Phe 
    370                 375                 380                 


Ile Thr Glu Leu Ile Ser Gly Gln His Thr Thr Ala Thr Gln Thr Ile 
385                 390                 395                 400 


Leu Gly Arg Asn Ile Phe Arg Val Asp Ser Gln Ala Cys Asn Leu Asn 
                405                 410                 415     


Asp Thr Thr Tyr Gly Val Asn Arg Ala Val Phe Tyr His Asp Ala Ser 
            420                 425                 430         


Glu Gly Ser Gln Arg Ser Val Tyr Glu Gly Tyr Ile Arg Thr Thr Gly 
        435                 440                 445             


Ile Asp Asn Pro Arg Val Gln Asn Ile Asn Thr Tyr Leu Pro Gly Glu 
    450                 455                 460                 


Asn Ser Asp Ile Pro Thr Pro Glu Asp Tyr Thr His Ile Leu Ser Thr 
465                 470                 475                 480 


Thr Ile Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Ser Asn Arg Arg 
                485                 490                 495     


Ser Ser Leu Val Met Tyr Gly Trp Thr His Lys Ser Leu Ala Arg Asn 
            500                 505                 510         


Asn Thr Ile Asn Pro Asp Arg Ile Thr Gln Ile Pro Leu Thr Lys Val 
        515                 520                 525             


Asp Thr Arg Gly Thr Gly Val Ser Tyr Val Asn Asp Pro Gly Phe Ile 
    530                 535                 540                 


Gly Gly Ala Leu Leu Gln Arg Thr Asp Asn Gly Ser Leu Gly Val Leu 
545                 550                 555                 560 


Arg Val Gln Phe Pro Leu His Leu Arg Gln Gln Tyr Arg Ile Arg Val 
                565                 570                 575     


Arg Tyr Ala Ser Thr Thr Asn Ile Gln Leu Ser Val Asn Gly Ser Phe 
            580                 585                 590         


Gly Thr Ile Ser Gln Asn Leu Pro Ser Thr Met Arg Leu Gly Glu Asp 
        595                 600                 605             


Leu Arg Tyr Gly Ser Phe Ala Ile Arg Glu Phe Ser Thr Ser Ile Arg 
    610                 615                 620                 


Pro Thr Ala Ser Pro Asp Gln Ile Arg Leu Thr Ile Glu Pro Ser Phe 
625                 630                 635                 640 


Ile Arg Gln Glu Val Tyr Val Asp Arg Ile Glu Phe Ile Pro Val Asn 
                645                 650                 655     


Pro Thr Arg Glu Ala Lys Glu Asp Leu Glu Ala Thr Lys Lys Ala Val 
            660                 665                 670         


Ala Ser Leu Phe Thr Arg Thr Arg Asp Gly Leu Gln Val Asn Val Thr 
        675                 680                 685             


Asp Tyr Gln Val Asp Gln Ala Ala Asn Leu Val Ser Cys Leu Ser Asp 
    690                 695                 700                 


Glu Gln Tyr Ala His Asp Lys Lys Met Leu Leu Glu Ala Val Arg Ala 
705                 710                 715                 720 


Ala Lys Arg Leu Ser Arg Glu Arg Asn Leu Leu Gln Asp Pro Asp Phe 
                725                 730                 735     


Asn Thr Ile Asn Ser Thr Glu Glu Asn Gly Trp Lys Ala Ser Asn Gly 
            740                 745                 750         


Val Thr Ile Ser Glu Gly Gly Pro Phe Tyr Lys Gly Arg Ala Leu Gln 
        755                 760                 765             


Leu Ala Ser Ala Arg Glu Asn Tyr Pro Thr Tyr Ile Tyr Gln Lys Val 
    770                 775                 780                 


Asp Ala Ser Glu Leu Lys Pro Tyr Thr Arg Tyr Arg Leu Asp Gly Phe 
785                 790                 795                 800 


Val Lys Ser Ser Gln Asp Leu Glu Ile Asp Leu Ile His His His Lys 
                805                 810                 815     


Val His Leu Val Lys Asn Ala Pro Asp Asn Leu Val Ser Asp Thr Tyr 
            820                 825                 830         


Pro Asp Asp Ser Cys Ser Gly Ile Asn Arg Cys Gln Glu Gln Gln Met 
        835                 840                 845             


Val Asn Ala Gln Leu Glu Thr Glu His His His Pro Met Asp Cys Cys 
    850                 855                 860                 


Gly Ala Ala Gln Thr His Glu Phe Ser Ser Tyr Ile Asp Thr Gly Asp 
865                 870                 875                 880 


Leu Asn Ser Thr Val Asp Gln Gly Ile Trp Ala Ile Phe Lys Val Arg 
                885                 890                 895     


Thr Thr Asp Gly Tyr Ala Thr Leu Gly Asn Leu Glu Leu Val Glu Ile 
            900                 905                 910         


Gly Pro Leu Ser Gly Glu Ser Leu Glu Arg Glu Gln Arg Asp Asn Ala 
        915                 920                 925             


Lys Trp Ser Ala Glu Leu Gly Arg Lys Arg Ala Glu Thr Asp Arg Val 
    930                 935                 940                 


Tyr Gln Asp Ala Lys Gln Ser Ile Asn His Leu Phe Val Asp Tyr Gln 
945                 950                 955                 960 


Asp Gln Gln Leu Asn Pro Glu Ile Gly Met Ala Asp Ile Met Asp Ala 
                965                 970                 975     


Gln Asn Leu Val Ala Ser Ile Ser Asp Val Tyr Ser Asp Ala Val Leu 
            980                 985                 990         


Gln Ile Pro Gly Ile Asn Tyr Glu  Ile Tyr Thr Glu Leu  Ser Asn Arg 
        995                 1000                 1005             


Leu Gln  Gln Ala Ser Tyr Leu  Tyr Thr Ser Arg Asn  Ala Val Gln 
    1010                 1015                 1020             


Asn Gly  Asp Phe Asn Ser Gly  Leu Asp Ser Trp Asn  Ala Thr Ala 
    1025                 1030                 1035             


Gly Ala  Thr Val Gln Gln Asp  Gly Asn Thr His Phe  Leu Val Leu 
    1040                 1045                 1050             


Ser His  Trp Asp Ala Gln Val  Ser Gln Gln Phe Arg  Val Gln Pro 
    1055                 1060                 1065             


Asn Cys  Lys Tyr Val Leu Arg  Val Thr Ala Glu Lys  Val Gly Gly 
    1070                 1075                 1080             


Gly Asp  Gly Tyr Val Thr Ile  Arg Asp Gly Ala His  His Thr Glu 
    1085                 1090                 1095             


Thr Leu  Thr Phe Asn Ala Cys  Asp Tyr Asp Ile Asn  Gly Thr Tyr 
    1100                 1105                 1110             


Val Thr  Asp Asn Thr Tyr Leu  Thr Lys Glu Val Val  Phe Tyr Ser 
    1115                 1120                 1125             


His Thr  Glu His Met Trp Val  Glu Val Ser Glu Thr  Glu Gly Val 
    1130                 1135                 1140             


Phe His  Ile Asp Ser Val Glu  Phe Met Glu Thr Gln  Gln 
    1145                 1150                 1155     


<210>  43
<211>  3471
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic bacterial coding sequence encoding TIC4064_25 
       comprising mutations at S33G, G87K, I385S, G402Q, and R604N.

<400>  43
atgaatcaaa ataaacacgg aattattggc gcttccaatt gtggttgtac gtcagataat       60

gttgcgaaat atcctttagc caacaatcca tattcaggag ctttaaattt aaattcttgt      120

caaaatagta gtattctcaa ctggattaac ataataggcg atgcagcaaa agaagcagta      180

tctattggga caacaatagt ctctcttatc acagcacctt ctcttactgg attaatttca      240

atagtatatg accttataaa aaaagtacta ggaggtagta gtggacaatc catatcagat      300

ttgtctatat gtgacttatt atctattatt gatttacggg taagtcagag tgttttaaat      360

gatgggattg cagattttaa tggttctgta ctcttataca ggaactattt agaggctctg      420

gatagctgga ataagaatcc taattctgct tctgctgaag aactccgtac tcgttttaga      480

atcgctgact cagaatttga tagaatttta acacgagggt ctttaacgaa tggtggctcg      540

ttagctagac aaaatgccca aatattatta ttaccttctt ttgcgagtgc tgcatttttc      600

catttattac tactaaggga tgctactaga tatggcacta attgggggct atacaatgct      660

acacctttta taaattatca atcaaaacta gtagagctta ttgaactata tactgattat      720

tgcgtacatt ggtataatcg aggtttcaac gaactaaggc aacgaggcac tagtgctaca      780

gcttggttag aatttcatag atatcgtaga gagatgacat tgatggtatt agatatagta      840

gcatcatttt caagtcttga tattactaat tacccaatag aaacagattt tcagttgagt      900

agggtcattt atacagatcc aattggtttt gtacatcgta gtagtcttag gggagaaagt      960

tggtttagct ttgttaatag agctaatttc tcagatttag aaaatgcaat acctaatcct     1020

agaccgtctt ggtttttaaa taatatgatt atatctactg gttcacttac attgccggtt     1080

agcccaagta ctgatagagc gagggtatgg tatggaagtc gagatcgaat ttcccctgct     1140

aattcacaat tttctactga actaatctct ggacaacata cgactgctac acaaactatt     1200

ttacaacgaa atatatttag agtagattct caagcttgta atttaaatga taccacatat     1260

ggagtgaata gggcggtatt ttatcatgat gcgagtgaag gttctcaaag atccgtgtac     1320

gaggggtata ttcgaacaac tgggatagat aaccctagag ttcaaaatat taacacttat     1380

ttacctggag aaaattcaga tatcccaact ccagaagact atactcatat attaagcaca     1440

acaataaatt taacaggagg acttagacaa gtagcatcta atcgccgttc atctttagta     1500

atgtatggtt ggacacataa aagtctggct cgtaacaata ccattaatcc agatagaatt     1560

acacagatac cattgacgaa ggttgatacc cgaggcacag gtgtttctta tgtgaatgat     1620

ccaggattta taggaggagc tctacttcaa aggactgacc atggttcgct tggagtattg     1680

agggtccaat ttccacttca cttaagacaa caatatcgca ttagagtccg ttatgcttct     1740

acaacaaata ttcgattgag tgtgaatggc agtttcggta ctatttctca aaatctccct     1800

agtacaatga atttaggaga ggatttaaga tacggatctt ttgctataag agagtttagt     1860

acttctatta gacccactgc aagtccggac caaattcgat tgacaataga accatctttt     1920

attagacaag aggtctatgt agatagaatt gagttcattc cagttaatcc gacgcgagag     1980

gcgaaagagg atctagaagc aacaaagaaa gcggtggcga gcttgtttac acgcacaagg     2040

gacggattac aagtaaatgt gacagattat caagtcgatc aagcggcaaa tttagtgtca     2100

tgcttatcag atgaacaata tgcgcatgat aaaaagatgt tattggaagc ggtacgcgcg     2160

gcaaaacgcc tcagccgaga acgcaactta cttcaggatc cagattttaa tacaatcaat     2220

agtacagaag aaaatggatg gaaagcaagt aacggcgtta ctattagcga aggcggtcca     2280

ttctataaag gccgtgcact tcagctagca agtgcacgag aaaattaccc aacatacatc     2340

tatcaaaaag tagatgcatc ggagttaaag ccgtatacac gttatagact ggatgggttc     2400

gtgaagagta gtcaagattt agaaattgat ctcattcacc atcataaagt ccatcttgtg     2460

aaaaatgcac cagataattt agtatctgat acttacccag atgattcttg tagtggaatc     2520

aatcgatgtc aggaacaaca gatggtaaat gcgcaactgg aaacagaaca tcatcatccg     2580

atggattgct gtggagcagc tcaaacacat gagttttctt cctatattga tacaggggat     2640

ttaaattcga ctgtagacca gggaatctgg gcgatcttta aagttcgaac aacagatggt     2700

tatgcgacgt taggaaatct tgaattggta gagatcggac cgttatcggg tgaatctcta     2760

gaacgtgaac aaagggataa tgcaaaatgg agtgcagagc taggaagaaa gcgtgcagaa     2820

acagatcgcg tgtatcaaga tgccaaacaa tccatcaatc atttatttgt ggattatcaa     2880

gatcaacaat taaatccaga aatagggatg gcagatatta tggacgctca aaatcttgtc     2940

gcatcaattt cagatgtata tagcgatgca gtactgcaaa tccctggaat taactatgag     3000

atttacacag agctatccaa tcgcttacaa caagcatcgt atctgtatac gtctcgaaat     3060

gcggtgcaaa atggggactt taacagcggt ctagatagtt ggaatgcaac agcgggtgct     3120

acggtacaac aggatggtaa tacgcatttc ttagttcttt ctcattggga tgcacaagtt     3180

tctcaacaat ttagagtgca gccaaattgt aaatatgtat tacgtgtaac agcagagaaa     3240

gtaggcggcg gagacggata cgtgacaatc cgggatggtg ctcatcatac agaaacgctt     3300

acatttaatg catgtgatta tgatataaat ggcacgtacg tgactgataa tacgtatcta     3360

acaaaagaag tggtattcta ttcacataca gaacacatgt gggtagaggt aagtgaaaca     3420

gaaggtgttt tccatataga cagtgttgag ttcatggaaa cccaacagta g              3471


<210>  44
<211>  1156
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of TIC4064_25 comprising mutations at S33G, 
       G87K, I385S, G402Q, and R604N.

<400>  44

Met Asn Gln Asn Lys His Gly Ile Ile Gly Ala Ser Asn Cys Gly Cys 
1               5                   10                  15      


Thr Ser Asp Asn Val Ala Lys Tyr Pro Leu Ala Asn Asn Pro Tyr Ser 
            20                  25                  30          


Gly Ala Leu Asn Leu Asn Ser Cys Gln Asn Ser Ser Ile Leu Asn Trp 
        35                  40                  45              


Ile Asn Ile Ile Gly Asp Ala Ala Lys Glu Ala Val Ser Ile Gly Thr 
    50                  55                  60                  


Thr Ile Val Ser Leu Ile Thr Ala Pro Ser Leu Thr Gly Leu Ile Ser 
65                  70                  75                  80  


Ile Val Tyr Asp Leu Ile Lys Lys Val Leu Gly Gly Ser Ser Gly Gln 
                85                  90                  95      


Ser Ile Ser Asp Leu Ser Ile Cys Asp Leu Leu Ser Ile Ile Asp Leu 
            100                 105                 110         


Arg Val Ser Gln Ser Val Leu Asn Asp Gly Ile Ala Asp Phe Asn Gly 
        115                 120                 125             


Ser Val Leu Leu Tyr Arg Asn Tyr Leu Glu Ala Leu Asp Ser Trp Asn 
    130                 135                 140                 


Lys Asn Pro Asn Ser Ala Ser Ala Glu Glu Leu Arg Thr Arg Phe Arg 
145                 150                 155                 160 


Ile Ala Asp Ser Glu Phe Asp Arg Ile Leu Thr Arg Gly Ser Leu Thr 
                165                 170                 175     


Asn Gly Gly Ser Leu Ala Arg Gln Asn Ala Gln Ile Leu Leu Leu Pro 
            180                 185                 190         


Ser Phe Ala Ser Ala Ala Phe Phe His Leu Leu Leu Leu Arg Asp Ala 
        195                 200                 205             


Thr Arg Tyr Gly Thr Asn Trp Gly Leu Tyr Asn Ala Thr Pro Phe Ile 
    210                 215                 220                 


Asn Tyr Gln Ser Lys Leu Val Glu Leu Ile Glu Leu Tyr Thr Asp Tyr 
225                 230                 235                 240 


Cys Val His Trp Tyr Asn Arg Gly Phe Asn Glu Leu Arg Gln Arg Gly 
                245                 250                 255     


Thr Ser Ala Thr Ala Trp Leu Glu Phe His Arg Tyr Arg Arg Glu Met 
            260                 265                 270         


Thr Leu Met Val Leu Asp Ile Val Ala Ser Phe Ser Ser Leu Asp Ile 
        275                 280                 285             


Thr Asn Tyr Pro Ile Glu Thr Asp Phe Gln Leu Ser Arg Val Ile Tyr 
    290                 295                 300                 


Thr Asp Pro Ile Gly Phe Val His Arg Ser Ser Leu Arg Gly Glu Ser 
305                 310                 315                 320 


Trp Phe Ser Phe Val Asn Arg Ala Asn Phe Ser Asp Leu Glu Asn Ala 
                325                 330                 335     


Ile Pro Asn Pro Arg Pro Ser Trp Phe Leu Asn Asn Met Ile Ile Ser 
            340                 345                 350         


Thr Gly Ser Leu Thr Leu Pro Val Ser Pro Ser Thr Asp Arg Ala Arg 
        355                 360                 365             


Val Trp Tyr Gly Ser Arg Asp Arg Ile Ser Pro Ala Asn Ser Gln Phe 
    370                 375                 380                 


Ser Thr Glu Leu Ile Ser Gly Gln His Thr Thr Ala Thr Gln Thr Ile 
385                 390                 395                 400 


Leu Gln Arg Asn Ile Phe Arg Val Asp Ser Gln Ala Cys Asn Leu Asn 
                405                 410                 415     


Asp Thr Thr Tyr Gly Val Asn Arg Ala Val Phe Tyr His Asp Ala Ser 
            420                 425                 430         


Glu Gly Ser Gln Arg Ser Val Tyr Glu Gly Tyr Ile Arg Thr Thr Gly 
        435                 440                 445             


Ile Asp Asn Pro Arg Val Gln Asn Ile Asn Thr Tyr Leu Pro Gly Glu 
    450                 455                 460                 


Asn Ser Asp Ile Pro Thr Pro Glu Asp Tyr Thr His Ile Leu Ser Thr 
465                 470                 475                 480 


Thr Ile Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Ser Asn Arg Arg 
                485                 490                 495     


Ser Ser Leu Val Met Tyr Gly Trp Thr His Lys Ser Leu Ala Arg Asn 
            500                 505                 510         


Asn Thr Ile Asn Pro Asp Arg Ile Thr Gln Ile Pro Leu Thr Lys Val 
        515                 520                 525             


Asp Thr Arg Gly Thr Gly Val Ser Tyr Val Asn Asp Pro Gly Phe Ile 
    530                 535                 540                 


Gly Gly Ala Leu Leu Gln Arg Thr Asp His Gly Ser Leu Gly Val Leu 
545                 550                 555                 560 


Arg Val Gln Phe Pro Leu His Leu Arg Gln Gln Tyr Arg Ile Arg Val 
                565                 570                 575     


Arg Tyr Ala Ser Thr Thr Asn Ile Arg Leu Ser Val Asn Gly Ser Phe 
            580                 585                 590         


Gly Thr Ile Ser Gln Asn Leu Pro Ser Thr Met Asn Leu Gly Glu Asp 
        595                 600                 605             


Leu Arg Tyr Gly Ser Phe Ala Ile Arg Glu Phe Ser Thr Ser Ile Arg 
    610                 615                 620                 


Pro Thr Ala Ser Pro Asp Gln Ile Arg Leu Thr Ile Glu Pro Ser Phe 
625                 630                 635                 640 


Ile Arg Gln Glu Val Tyr Val Asp Arg Ile Glu Phe Ile Pro Val Asn 
                645                 650                 655     


Pro Thr Arg Glu Ala Lys Glu Asp Leu Glu Ala Thr Lys Lys Ala Val 
            660                 665                 670         


Ala Ser Leu Phe Thr Arg Thr Arg Asp Gly Leu Gln Val Asn Val Thr 
        675                 680                 685             


Asp Tyr Gln Val Asp Gln Ala Ala Asn Leu Val Ser Cys Leu Ser Asp 
    690                 695                 700                 


Glu Gln Tyr Ala His Asp Lys Lys Met Leu Leu Glu Ala Val Arg Ala 
705                 710                 715                 720 


Ala Lys Arg Leu Ser Arg Glu Arg Asn Leu Leu Gln Asp Pro Asp Phe 
                725                 730                 735     


Asn Thr Ile Asn Ser Thr Glu Glu Asn Gly Trp Lys Ala Ser Asn Gly 
            740                 745                 750         


Val Thr Ile Ser Glu Gly Gly Pro Phe Tyr Lys Gly Arg Ala Leu Gln 
        755                 760                 765             


Leu Ala Ser Ala Arg Glu Asn Tyr Pro Thr Tyr Ile Tyr Gln Lys Val 
    770                 775                 780                 


Asp Ala Ser Glu Leu Lys Pro Tyr Thr Arg Tyr Arg Leu Asp Gly Phe 
785                 790                 795                 800 


Val Lys Ser Ser Gln Asp Leu Glu Ile Asp Leu Ile His His His Lys 
                805                 810                 815     


Val His Leu Val Lys Asn Ala Pro Asp Asn Leu Val Ser Asp Thr Tyr 
            820                 825                 830         


Pro Asp Asp Ser Cys Ser Gly Ile Asn Arg Cys Gln Glu Gln Gln Met 
        835                 840                 845             


Val Asn Ala Gln Leu Glu Thr Glu His His His Pro Met Asp Cys Cys 
    850                 855                 860                 


Gly Ala Ala Gln Thr His Glu Phe Ser Ser Tyr Ile Asp Thr Gly Asp 
865                 870                 875                 880 


Leu Asn Ser Thr Val Asp Gln Gly Ile Trp Ala Ile Phe Lys Val Arg 
                885                 890                 895     


Thr Thr Asp Gly Tyr Ala Thr Leu Gly Asn Leu Glu Leu Val Glu Ile 
            900                 905                 910         


Gly Pro Leu Ser Gly Glu Ser Leu Glu Arg Glu Gln Arg Asp Asn Ala 
        915                 920                 925             


Lys Trp Ser Ala Glu Leu Gly Arg Lys Arg Ala Glu Thr Asp Arg Val 
    930                 935                 940                 


Tyr Gln Asp Ala Lys Gln Ser Ile Asn His Leu Phe Val Asp Tyr Gln 
945                 950                 955                 960 


Asp Gln Gln Leu Asn Pro Glu Ile Gly Met Ala Asp Ile Met Asp Ala 
                965                 970                 975     


Gln Asn Leu Val Ala Ser Ile Ser Asp Val Tyr Ser Asp Ala Val Leu 
            980                 985                 990         


Gln Ile Pro Gly Ile Asn Tyr Glu  Ile Tyr Thr Glu Leu  Ser Asn Arg 
        995                 1000                 1005             


Leu Gln  Gln Ala Ser Tyr Leu  Tyr Thr Ser Arg Asn  Ala Val Gln 
    1010                 1015                 1020             


Asn Gly  Asp Phe Asn Ser Gly  Leu Asp Ser Trp Asn  Ala Thr Ala 
    1025                 1030                 1035             


Gly Ala  Thr Val Gln Gln Asp  Gly Asn Thr His Phe  Leu Val Leu 
    1040                 1045                 1050             


Ser His  Trp Asp Ala Gln Val  Ser Gln Gln Phe Arg  Val Gln Pro 
    1055                 1060                 1065             


Asn Cys  Lys Tyr Val Leu Arg  Val Thr Ala Glu Lys  Val Gly Gly 
    1070                 1075                 1080             


Gly Asp  Gly Tyr Val Thr Ile  Arg Asp Gly Ala His  His Thr Glu 
    1085                 1090                 1095             


Thr Leu  Thr Phe Asn Ala Cys  Asp Tyr Asp Ile Asn  Gly Thr Tyr 
    1100                 1105                 1110             


Val Thr  Asp Asn Thr Tyr Leu  Thr Lys Glu Val Val  Phe Tyr Ser 
    1115                 1120                 1125             


His Thr  Glu His Met Trp Val  Glu Val Ser Glu Thr  Glu Gly Val 
    1130                 1135                 1140             


Phe His  Ile Asp Ser Val Glu  Phe Met Glu Thr Gln  Gln 
    1145                 1150                 1155     


<210>  45
<211>  3471
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic bacterial coding sequence encoding TIC4064_26 
       comprising mutations at G87K, F199Y, V325A, S331A, and Q631T.

<400>  45
atgaatcaaa ataaacacgg aattattggc gcttccaatt gtggttgtac gtcagataat       60

gttgcgaaat atcctttagc caacaatcca tattcatctg ctttaaattt aaattcttgt      120

caaaatagta gtattctcaa ctggattaac ataataggcg atgcagcaaa agaagcagta      180

tctattggga caacaatagt ctctcttatc acagcacctt ctcttactgg attaatttca      240

atagtatatg accttataaa aaaagtacta ggaggtagta gtggacaatc catatcagat      300

ttgtctatat gtgacttatt atctattatt gatttacggg taagtcagag tgttttaaat      360

gatgggattg cagattttaa tggttctgta ctcttataca ggaactattt agaggctctg      420

gatagctgga ataagaatcc taattctgct tctgctgaag aactccgtac tcgttttaga      480

atcgctgact cagaatttga tagaatttta acacgagggt ctttaacgaa tggtggctcg      540

ttagctagac aaaatgccca aatattatta ttaccttctt ttgcgagtgc tgcatatttc      600

catttattac tactaaggga tgctactaga tatggcacta attgggggct atacaatgct      660

acacctttta taaattatca atcaaaacta gtagagctta ttgaactata tactgattat      720

tgcgtacatt ggtataatcg aggtttcaac gaactaaggc aacgaggcac tagtgctaca      780

gcttggttag aatttcatag atatcgtaga gagatgacat tgatggtatt agatatagta      840

gcatcatttt caagtcttga tattactaat tacccaatag aaacagattt tcagttgagt      900

agggtcattt atacagatcc aattggtttt gtacatcgta gtagtcttag gggagaaagt      960

tggtttagct ttgcaaatag agctaatttc gcagatttag aaaatgcaat acctaatcct     1020

agaccgtctt ggtttttaaa taatatgatt atatctactg gttcacttac attgccggtt     1080

agcccaagta ctgatagagc gagggtatgg tatggaagtc gagatcgaat ttcccctgct     1140

aattcacaat ttattactga actaatctct ggacaacata cgactgctac acaaactatt     1200

ttagggcgaa atatatttag agtagattct caagcttgta atttaaatga taccacatat     1260

ggagtgaata gggcggtatt ttatcatgat gcgagtgaag gttctcaaag atccgtgtac     1320

gaggggtata ttcgaacaac tgggatagat aaccctagag ttcaaaatat taacacttat     1380

ttacctggag aaaattcaga tatcccaact ccagaagact atactcatat attaagcaca     1440

acaataaatt taacaggagg acttagacaa gtagcatcta atcgccgttc atctttagta     1500

atgtatggtt ggacacataa aagtctggct cgtaacaata ccattaatcc agatagaatt     1560

acacagatac cattgacgaa ggttgatacc cgaggcacag gtgtttctta tgtgaatgat     1620

ccaggattta taggaggagc tctacttcaa aggactgacc atggttcgct tggagtattg     1680

agggtccaat ttccacttca cttaagacaa caatatcgca ttagagtccg ttatgcttct     1740

acaacaaata ttcgattgag tgtgaatggc agtttcggta ctatttctca aaatctccct     1800

agtacaatga gattaggaga ggatttaaga tacggatctt ttgctataag agagtttagt     1860

acttctatta gacccactgc aagtccggac acaattcgat tgacaataga accatctttt     1920

attagacaag aggtctatgt agatagaatt gagttcattc cagttaatcc gacgcgagag     1980

gcgaaagagg atctagaagc aacaaagaaa gcggtggcga gcttgtttac acgcacaagg     2040

gacggattac aagtaaatgt gacagattat caagtcgatc aagcggcaaa tttagtgtca     2100

tgcttatcag atgaacaata tgcgcatgat aaaaagatgt tattggaagc ggtacgcgcg     2160

gcaaaacgcc tcagccgaga acgcaactta cttcaggatc cagattttaa tacaatcaat     2220

agtacagaag aaaatggatg gaaagcaagt aacggcgtta ctattagcga aggcggtcca     2280

ttctataaag gccgtgcact tcagctagca agtgcacgag aaaattaccc aacatacatc     2340

tatcaaaaag tagatgcatc ggagttaaag ccgtatacac gttatagact ggatgggttc     2400

gtgaagagta gtcaagattt agaaattgat ctcattcacc atcataaagt ccatcttgtg     2460

aaaaatgcac cagataattt agtatctgat acttacccag atgattcttg tagtggaatc     2520

aatcgatgtc aggaacaaca gatggtaaat gcgcaactgg aaacagaaca tcatcatccg     2580

atggattgct gtggagcagc tcaaacacat gagttttctt cctatattga tacaggggat     2640

ttaaattcga ctgtagacca gggaatctgg gcgatcttta aagttcgaac aacagatggt     2700

tatgcgacgt taggaaatct tgaattggta gagatcggac cgttatcggg tgaatctcta     2760

gaacgtgaac aaagggataa tgcaaaatgg agtgcagagc taggaagaaa gcgtgcagaa     2820

acagatcgcg tgtatcaaga tgccaaacaa tccatcaatc atttatttgt ggattatcaa     2880

gatcaacaat taaatccaga aatagggatg gcagatatta tggacgctca aaatcttgtc     2940

gcatcaattt cagatgtata tagcgatgca gtactgcaaa tccctggaat taactatgag     3000

atttacacag agctatccaa tcgcttacaa caagcatcgt atctgtatac gtctcgaaat     3060

gcggtgcaaa atggggactt taacagcggt ctagatagtt ggaatgcaac agcgggtgct     3120

acggtacaac aggatggtaa tacgcatttc ttagttcttt ctcattggga tgcacaagtt     3180

tctcaacaat ttagagtgca gccaaattgt aaatatgtat tacgtgtaac agcagagaaa     3240

gtaggcggcg gagacggata cgtgacaatc cgggatggtg ctcatcatac agaaacgctt     3300

acatttaatg catgtgatta tgatataaat ggcacgtacg tgactgataa tacgtatcta     3360

acaaaagaag tggtattcta ttcacataca gaacacatgt gggtagaggt aagtgaaaca     3420

gaaggtgttt tccatataga cagtgttgag ttcatggaaa cccaacagta g              3471


<210>  46
<211>  1156
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of TIC4064_26 comprising mutations at G87K, 
       F199Y, V325A, S331A, and Q631T.

<400>  46

Met Asn Gln Asn Lys His Gly Ile Ile Gly Ala Ser Asn Cys Gly Cys 
1               5                   10                  15      


Thr Ser Asp Asn Val Ala Lys Tyr Pro Leu Ala Asn Asn Pro Tyr Ser 
            20                  25                  30          


Ser Ala Leu Asn Leu Asn Ser Cys Gln Asn Ser Ser Ile Leu Asn Trp 
        35                  40                  45              


Ile Asn Ile Ile Gly Asp Ala Ala Lys Glu Ala Val Ser Ile Gly Thr 
    50                  55                  60                  


Thr Ile Val Ser Leu Ile Thr Ala Pro Ser Leu Thr Gly Leu Ile Ser 
65                  70                  75                  80  


Ile Val Tyr Asp Leu Ile Lys Lys Val Leu Gly Gly Ser Ser Gly Gln 
                85                  90                  95      


Ser Ile Ser Asp Leu Ser Ile Cys Asp Leu Leu Ser Ile Ile Asp Leu 
            100                 105                 110         


Arg Val Ser Gln Ser Val Leu Asn Asp Gly Ile Ala Asp Phe Asn Gly 
        115                 120                 125             


Ser Val Leu Leu Tyr Arg Asn Tyr Leu Glu Ala Leu Asp Ser Trp Asn 
    130                 135                 140                 


Lys Asn Pro Asn Ser Ala Ser Ala Glu Glu Leu Arg Thr Arg Phe Arg 
145                 150                 155                 160 


Ile Ala Asp Ser Glu Phe Asp Arg Ile Leu Thr Arg Gly Ser Leu Thr 
                165                 170                 175     


Asn Gly Gly Ser Leu Ala Arg Gln Asn Ala Gln Ile Leu Leu Leu Pro 
            180                 185                 190         


Ser Phe Ala Ser Ala Ala Tyr Phe His Leu Leu Leu Leu Arg Asp Ala 
        195                 200                 205             


Thr Arg Tyr Gly Thr Asn Trp Gly Leu Tyr Asn Ala Thr Pro Phe Ile 
    210                 215                 220                 


Asn Tyr Gln Ser Lys Leu Val Glu Leu Ile Glu Leu Tyr Thr Asp Tyr 
225                 230                 235                 240 


Cys Val His Trp Tyr Asn Arg Gly Phe Asn Glu Leu Arg Gln Arg Gly 
                245                 250                 255     


Thr Ser Ala Thr Ala Trp Leu Glu Phe His Arg Tyr Arg Arg Glu Met 
            260                 265                 270         


Thr Leu Met Val Leu Asp Ile Val Ala Ser Phe Ser Ser Leu Asp Ile 
        275                 280                 285             


Thr Asn Tyr Pro Ile Glu Thr Asp Phe Gln Leu Ser Arg Val Ile Tyr 
    290                 295                 300                 


Thr Asp Pro Ile Gly Phe Val His Arg Ser Ser Leu Arg Gly Glu Ser 
305                 310                 315                 320 


Trp Phe Ser Phe Ala Asn Arg Ala Asn Phe Ala Asp Leu Glu Asn Ala 
                325                 330                 335     


Ile Pro Asn Pro Arg Pro Ser Trp Phe Leu Asn Asn Met Ile Ile Ser 
            340                 345                 350         


Thr Gly Ser Leu Thr Leu Pro Val Ser Pro Ser Thr Asp Arg Ala Arg 
        355                 360                 365             


Val Trp Tyr Gly Ser Arg Asp Arg Ile Ser Pro Ala Asn Ser Gln Phe 
    370                 375                 380                 


Ile Thr Glu Leu Ile Ser Gly Gln His Thr Thr Ala Thr Gln Thr Ile 
385                 390                 395                 400 


Leu Gly Arg Asn Ile Phe Arg Val Asp Ser Gln Ala Cys Asn Leu Asn 
                405                 410                 415     


Asp Thr Thr Tyr Gly Val Asn Arg Ala Val Phe Tyr His Asp Ala Ser 
            420                 425                 430         


Glu Gly Ser Gln Arg Ser Val Tyr Glu Gly Tyr Ile Arg Thr Thr Gly 
        435                 440                 445             


Ile Asp Asn Pro Arg Val Gln Asn Ile Asn Thr Tyr Leu Pro Gly Glu 
    450                 455                 460                 


Asn Ser Asp Ile Pro Thr Pro Glu Asp Tyr Thr His Ile Leu Ser Thr 
465                 470                 475                 480 


Thr Ile Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Ser Asn Arg Arg 
                485                 490                 495     


Ser Ser Leu Val Met Tyr Gly Trp Thr His Lys Ser Leu Ala Arg Asn 
            500                 505                 510         


Asn Thr Ile Asn Pro Asp Arg Ile Thr Gln Ile Pro Leu Thr Lys Val 
        515                 520                 525             


Asp Thr Arg Gly Thr Gly Val Ser Tyr Val Asn Asp Pro Gly Phe Ile 
    530                 535                 540                 


Gly Gly Ala Leu Leu Gln Arg Thr Asp His Gly Ser Leu Gly Val Leu 
545                 550                 555                 560 


Arg Val Gln Phe Pro Leu His Leu Arg Gln Gln Tyr Arg Ile Arg Val 
                565                 570                 575     


Arg Tyr Ala Ser Thr Thr Asn Ile Arg Leu Ser Val Asn Gly Ser Phe 
            580                 585                 590         


Gly Thr Ile Ser Gln Asn Leu Pro Ser Thr Met Arg Leu Gly Glu Asp 
        595                 600                 605             


Leu Arg Tyr Gly Ser Phe Ala Ile Arg Glu Phe Ser Thr Ser Ile Arg 
    610                 615                 620                 


Pro Thr Ala Ser Pro Asp Thr Ile Arg Leu Thr Ile Glu Pro Ser Phe 
625                 630                 635                 640 


Ile Arg Gln Glu Val Tyr Val Asp Arg Ile Glu Phe Ile Pro Val Asn 
                645                 650                 655     


Pro Thr Arg Glu Ala Lys Glu Asp Leu Glu Ala Thr Lys Lys Ala Val 
            660                 665                 670         


Ala Ser Leu Phe Thr Arg Thr Arg Asp Gly Leu Gln Val Asn Val Thr 
        675                 680                 685             


Asp Tyr Gln Val Asp Gln Ala Ala Asn Leu Val Ser Cys Leu Ser Asp 
    690                 695                 700                 


Glu Gln Tyr Ala His Asp Lys Lys Met Leu Leu Glu Ala Val Arg Ala 
705                 710                 715                 720 


Ala Lys Arg Leu Ser Arg Glu Arg Asn Leu Leu Gln Asp Pro Asp Phe 
                725                 730                 735     


Asn Thr Ile Asn Ser Thr Glu Glu Asn Gly Trp Lys Ala Ser Asn Gly 
            740                 745                 750         


Val Thr Ile Ser Glu Gly Gly Pro Phe Tyr Lys Gly Arg Ala Leu Gln 
        755                 760                 765             


Leu Ala Ser Ala Arg Glu Asn Tyr Pro Thr Tyr Ile Tyr Gln Lys Val 
    770                 775                 780                 


Asp Ala Ser Glu Leu Lys Pro Tyr Thr Arg Tyr Arg Leu Asp Gly Phe 
785                 790                 795                 800 


Val Lys Ser Ser Gln Asp Leu Glu Ile Asp Leu Ile His His His Lys 
                805                 810                 815     


Val His Leu Val Lys Asn Ala Pro Asp Asn Leu Val Ser Asp Thr Tyr 
            820                 825                 830         


Pro Asp Asp Ser Cys Ser Gly Ile Asn Arg Cys Gln Glu Gln Gln Met 
        835                 840                 845             


Val Asn Ala Gln Leu Glu Thr Glu His His His Pro Met Asp Cys Cys 
    850                 855                 860                 


Gly Ala Ala Gln Thr His Glu Phe Ser Ser Tyr Ile Asp Thr Gly Asp 
865                 870                 875                 880 


Leu Asn Ser Thr Val Asp Gln Gly Ile Trp Ala Ile Phe Lys Val Arg 
                885                 890                 895     


Thr Thr Asp Gly Tyr Ala Thr Leu Gly Asn Leu Glu Leu Val Glu Ile 
            900                 905                 910         


Gly Pro Leu Ser Gly Glu Ser Leu Glu Arg Glu Gln Arg Asp Asn Ala 
        915                 920                 925             


Lys Trp Ser Ala Glu Leu Gly Arg Lys Arg Ala Glu Thr Asp Arg Val 
    930                 935                 940                 


Tyr Gln Asp Ala Lys Gln Ser Ile Asn His Leu Phe Val Asp Tyr Gln 
945                 950                 955                 960 


Asp Gln Gln Leu Asn Pro Glu Ile Gly Met Ala Asp Ile Met Asp Ala 
                965                 970                 975     


Gln Asn Leu Val Ala Ser Ile Ser Asp Val Tyr Ser Asp Ala Val Leu 
            980                 985                 990         


Gln Ile Pro Gly Ile Asn Tyr Glu  Ile Tyr Thr Glu Leu  Ser Asn Arg 
        995                 1000                 1005             


Leu Gln  Gln Ala Ser Tyr Leu  Tyr Thr Ser Arg Asn  Ala Val Gln 
    1010                 1015                 1020             


Asn Gly  Asp Phe Asn Ser Gly  Leu Asp Ser Trp Asn  Ala Thr Ala 
    1025                 1030                 1035             


Gly Ala  Thr Val Gln Gln Asp  Gly Asn Thr His Phe  Leu Val Leu 
    1040                 1045                 1050             


Ser His  Trp Asp Ala Gln Val  Ser Gln Gln Phe Arg  Val Gln Pro 
    1055                 1060                 1065             


Asn Cys  Lys Tyr Val Leu Arg  Val Thr Ala Glu Lys  Val Gly Gly 
    1070                 1075                 1080             


Gly Asp  Gly Tyr Val Thr Ile  Arg Asp Gly Ala His  His Thr Glu 
    1085                 1090                 1095             


Thr Leu  Thr Phe Asn Ala Cys  Asp Tyr Asp Ile Asn  Gly Thr Tyr 
    1100                 1105                 1110             


Val Thr  Asp Asn Thr Tyr Leu  Thr Lys Glu Val Val  Phe Tyr Ser 
    1115                 1120                 1125             


His Thr  Glu His Met Trp Val  Glu Val Ser Glu Thr  Glu Gly Val 
    1130                 1135                 1140             


Phe His  Ile Asp Ser Val Glu  Phe Met Glu Thr Gln  Gln 
    1145                 1150                 1155     


<210>  47
<211>  3471
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic bacterial coding sequence encoding TIC4064_27 
       comprising mutations at G87S, I308C, V325A, S331A, and Q631T.

<400>  47
atgaatcaaa ataaacacgg aattattggc gcttccaatt gtggttgtac gtcagataat       60

gttgcgaaat atcctttagc caacaatcca tattcatctg ctttaaattt aaattcttgt      120

caaaatagta gtattctcaa ctggattaac ataataggcg atgcagcaaa agaagcagta      180

tctattggga caacaatagt ctctcttatc acagcacctt ctcttactgg attaatttca      240

atagtatatg accttatatc taaagtacta ggaggtagta gtggacaatc catatcagat      300

ttgtctatat gtgacttatt atctattatt gatttacggg taagtcagag tgttttaaat      360

gatgggattg cagattttaa tggttctgta ctcttataca ggaactattt agaggctctg      420

gatagctgga ataagaatcc taattctgct tctgctgaag aactccgtac tcgttttaga      480

atcgctgact cagaatttga tagaatttta acacgagggt ctttaacgaa tggtggctcg      540

ttagctagac aaaatgccca aatattatta ttaccttctt ttgcgagtgc tgcatttttc      600

catttattac tactaaggga tgctactaga tatggcacta attgggggct atacaatgct      660

acacctttta taaattatca atcaaaacta gtagagctta ttgaactata tactgattat      720

tgcgtacatt ggtataatcg aggtttcaac gaactaaggc aacgaggcac tagtgctaca      780

gcttggttag aatttcatag atatcgtaga gagatgacat tgatggtatt agatatagta      840

gcatcatttt caagtcttga tattactaat tacccaatag aaacagattt tcagttgagt      900

agggtcattt atacagatcc atgtggtttt gtacatcgta gtagtcttag gggagaaagt      960

tggtttagct ttgcaaatag agctaatttc gcagatttag aaaatgcaat acctaatcct     1020

agaccgtctt ggtttttaaa taatatgatt atatctactg gttcacttac attgccggtt     1080

agcccaagta ctgatagagc gagggtatgg tatggaagtc gagatcgaat ttcccctgct     1140

aattcacaat ttattactga actaatctct ggacaacata cgactgctac acaaactatt     1200

ttagggcgaa atatatttag agtagattct caagcttgta atttaaatga taccacatat     1260

ggagtgaata gggcggtatt ttatcatgat gcgagtgaag gttctcaaag atccgtgtac     1320

gaggggtata ttcgaacaac tgggatagat aaccctagag ttcaaaatat taacacttat     1380

ttacctggag aaaattcaga tatcccaact ccagaagact atactcatat attaagcaca     1440

acaataaatt taacaggagg acttagacaa gtagcatcta atcgccgttc atctttagta     1500

atgtatggtt ggacacataa aagtctggct cgtaacaata ccattaatcc agatagaatt     1560

acacagatac cattgacgaa ggttgatacc cgaggcacag gtgtttctta tgtgaatgat     1620

ccaggattta taggaggagc tctacttcaa aggactgacc atggttcgct tggagtattg     1680

agggtccaat ttccacttca cttaagacaa caatatcgca ttagagtccg ttatgcttct     1740

acaacaaata ttcgattgag tgtgaatggc agtttcggta ctatttctca aaatctccct     1800

agtacaatga gattaggaga ggatttaaga tacggatctt ttgctataag agagtttagt     1860

acttctatta gacccactgc aagtccggac acaattcgat tgacaataga accatctttt     1920

attagacaag aggtctatgt agatagaatt gagttcattc cagttaatcc gacgcgagag     1980

gcgaaagagg atctagaagc aacaaagaaa gcggtggcga gcttgtttac acgcacaagg     2040

gacggattac aagtaaatgt gacagattat caagtcgatc aagcggcaaa tttagtgtca     2100

tgcttatcag atgaacaata tgcgcatgat aaaaagatgt tattggaagc ggtacgcgcg     2160

gcaaaacgcc tcagccgaga acgcaactta cttcaggatc cagattttaa tacaatcaat     2220

agtacagaag aaaatggatg gaaagcaagt aacggcgtta ctattagcga aggcggtcca     2280

ttctataaag gccgtgcact tcagctagca agtgcacgag aaaattaccc aacatacatc     2340

tatcaaaaag tagatgcatc ggagttaaag ccgtatacac gttatagact ggatgggttc     2400

gtgaagagta gtcaagattt agaaattgat ctcattcacc atcataaagt ccatcttgtg     2460

aaaaatgcac cagataattt agtatctgat acttacccag atgattcttg tagtggaatc     2520

aatcgatgtc aggaacaaca gatggtaaat gcgcaactgg aaacagaaca tcatcatccg     2580

atggattgct gtggagcagc tcaaacacat gagttttctt cctatattga tacaggggat     2640

ttaaattcga ctgtagacca gggaatctgg gcgatcttta aagttcgaac aacagatggt     2700

tatgcgacgt taggaaatct tgaattggta gagatcggac cgttatcggg tgaatctcta     2760

gaacgtgaac aaagggataa tgcaaaatgg agtgcagagc taggaagaaa gcgtgcagaa     2820

acagatcgcg tgtatcaaga tgccaaacaa tccatcaatc atttatttgt ggattatcaa     2880

gatcaacaat taaatccaga aatagggatg gcagatatta tggacgctca aaatcttgtc     2940

gcatcaattt cagatgtata tagcgatgca gtactgcaaa tccctggaat taactatgag     3000

atttacacag agctatccaa tcgcttacaa caagcatcgt atctgtatac gtctcgaaat     3060

gcggtgcaaa atggggactt taacagcggt ctagatagtt ggaatgcaac agcgggtgct     3120

acggtacaac aggatggtaa tacgcatttc ttagttcttt ctcattggga tgcacaagtt     3180

tctcaacaat ttagagtgca gccaaattgt aaatatgtat tacgtgtaac agcagagaaa     3240

gtaggcggcg gagacggata cgtgacaatc cgggatggtg ctcatcatac agaaacgctt     3300

acatttaatg catgtgatta tgatataaat ggcacgtacg tgactgataa tacgtatcta     3360

acaaaagaag tggtattcta ttcacataca gaacacatgt gggtagaggt aagtgaaaca     3420

gaaggtgttt tccatataga cagtgttgag ttcatggaaa cccaacagta g              3471


<210>  48
<211>  1156
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of TIC4064_27 comprising mutations at G87S, 
       I308C, V325A, S331A, and Q631T.

<400>  48

Met Asn Gln Asn Lys His Gly Ile Ile Gly Ala Ser Asn Cys Gly Cys 
1               5                   10                  15      


Thr Ser Asp Asn Val Ala Lys Tyr Pro Leu Ala Asn Asn Pro Tyr Ser 
            20                  25                  30          


Ser Ala Leu Asn Leu Asn Ser Cys Gln Asn Ser Ser Ile Leu Asn Trp 
        35                  40                  45              


Ile Asn Ile Ile Gly Asp Ala Ala Lys Glu Ala Val Ser Ile Gly Thr 
    50                  55                  60                  


Thr Ile Val Ser Leu Ile Thr Ala Pro Ser Leu Thr Gly Leu Ile Ser 
65                  70                  75                  80  


Ile Val Tyr Asp Leu Ile Ser Lys Val Leu Gly Gly Ser Ser Gly Gln 
                85                  90                  95      


Ser Ile Ser Asp Leu Ser Ile Cys Asp Leu Leu Ser Ile Ile Asp Leu 
            100                 105                 110         


Arg Val Ser Gln Ser Val Leu Asn Asp Gly Ile Ala Asp Phe Asn Gly 
        115                 120                 125             


Ser Val Leu Leu Tyr Arg Asn Tyr Leu Glu Ala Leu Asp Ser Trp Asn 
    130                 135                 140                 


Lys Asn Pro Asn Ser Ala Ser Ala Glu Glu Leu Arg Thr Arg Phe Arg 
145                 150                 155                 160 


Ile Ala Asp Ser Glu Phe Asp Arg Ile Leu Thr Arg Gly Ser Leu Thr 
                165                 170                 175     


Asn Gly Gly Ser Leu Ala Arg Gln Asn Ala Gln Ile Leu Leu Leu Pro 
            180                 185                 190         


Ser Phe Ala Ser Ala Ala Phe Phe His Leu Leu Leu Leu Arg Asp Ala 
        195                 200                 205             


Thr Arg Tyr Gly Thr Asn Trp Gly Leu Tyr Asn Ala Thr Pro Phe Ile 
    210                 215                 220                 


Asn Tyr Gln Ser Lys Leu Val Glu Leu Ile Glu Leu Tyr Thr Asp Tyr 
225                 230                 235                 240 


Cys Val His Trp Tyr Asn Arg Gly Phe Asn Glu Leu Arg Gln Arg Gly 
                245                 250                 255     


Thr Ser Ala Thr Ala Trp Leu Glu Phe His Arg Tyr Arg Arg Glu Met 
            260                 265                 270         


Thr Leu Met Val Leu Asp Ile Val Ala Ser Phe Ser Ser Leu Asp Ile 
        275                 280                 285             


Thr Asn Tyr Pro Ile Glu Thr Asp Phe Gln Leu Ser Arg Val Ile Tyr 
    290                 295                 300                 


Thr Asp Pro Cys Gly Phe Val His Arg Ser Ser Leu Arg Gly Glu Ser 
305                 310                 315                 320 


Trp Phe Ser Phe Ala Asn Arg Ala Asn Phe Ala Asp Leu Glu Asn Ala 
                325                 330                 335     


Ile Pro Asn Pro Arg Pro Ser Trp Phe Leu Asn Asn Met Ile Ile Ser 
            340                 345                 350         


Thr Gly Ser Leu Thr Leu Pro Val Ser Pro Ser Thr Asp Arg Ala Arg 
        355                 360                 365             


Val Trp Tyr Gly Ser Arg Asp Arg Ile Ser Pro Ala Asn Ser Gln Phe 
    370                 375                 380                 


Ile Thr Glu Leu Ile Ser Gly Gln His Thr Thr Ala Thr Gln Thr Ile 
385                 390                 395                 400 


Leu Gly Arg Asn Ile Phe Arg Val Asp Ser Gln Ala Cys Asn Leu Asn 
                405                 410                 415     


Asp Thr Thr Tyr Gly Val Asn Arg Ala Val Phe Tyr His Asp Ala Ser 
            420                 425                 430         


Glu Gly Ser Gln Arg Ser Val Tyr Glu Gly Tyr Ile Arg Thr Thr Gly 
        435                 440                 445             


Ile Asp Asn Pro Arg Val Gln Asn Ile Asn Thr Tyr Leu Pro Gly Glu 
    450                 455                 460                 


Asn Ser Asp Ile Pro Thr Pro Glu Asp Tyr Thr His Ile Leu Ser Thr 
465                 470                 475                 480 


Thr Ile Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Ser Asn Arg Arg 
                485                 490                 495     


Ser Ser Leu Val Met Tyr Gly Trp Thr His Lys Ser Leu Ala Arg Asn 
            500                 505                 510         


Asn Thr Ile Asn Pro Asp Arg Ile Thr Gln Ile Pro Leu Thr Lys Val 
        515                 520                 525             


Asp Thr Arg Gly Thr Gly Val Ser Tyr Val Asn Asp Pro Gly Phe Ile 
    530                 535                 540                 


Gly Gly Ala Leu Leu Gln Arg Thr Asp His Gly Ser Leu Gly Val Leu 
545                 550                 555                 560 


Arg Val Gln Phe Pro Leu His Leu Arg Gln Gln Tyr Arg Ile Arg Val 
                565                 570                 575     


Arg Tyr Ala Ser Thr Thr Asn Ile Arg Leu Ser Val Asn Gly Ser Phe 
            580                 585                 590         


Gly Thr Ile Ser Gln Asn Leu Pro Ser Thr Met Arg Leu Gly Glu Asp 
        595                 600                 605             


Leu Arg Tyr Gly Ser Phe Ala Ile Arg Glu Phe Ser Thr Ser Ile Arg 
    610                 615                 620                 


Pro Thr Ala Ser Pro Asp Thr Ile Arg Leu Thr Ile Glu Pro Ser Phe 
625                 630                 635                 640 


Ile Arg Gln Glu Val Tyr Val Asp Arg Ile Glu Phe Ile Pro Val Asn 
                645                 650                 655     


Pro Thr Arg Glu Ala Lys Glu Asp Leu Glu Ala Thr Lys Lys Ala Val 
            660                 665                 670         


Ala Ser Leu Phe Thr Arg Thr Arg Asp Gly Leu Gln Val Asn Val Thr 
        675                 680                 685             


Asp Tyr Gln Val Asp Gln Ala Ala Asn Leu Val Ser Cys Leu Ser Asp 
    690                 695                 700                 


Glu Gln Tyr Ala His Asp Lys Lys Met Leu Leu Glu Ala Val Arg Ala 
705                 710                 715                 720 


Ala Lys Arg Leu Ser Arg Glu Arg Asn Leu Leu Gln Asp Pro Asp Phe 
                725                 730                 735     


Asn Thr Ile Asn Ser Thr Glu Glu Asn Gly Trp Lys Ala Ser Asn Gly 
            740                 745                 750         


Val Thr Ile Ser Glu Gly Gly Pro Phe Tyr Lys Gly Arg Ala Leu Gln 
        755                 760                 765             


Leu Ala Ser Ala Arg Glu Asn Tyr Pro Thr Tyr Ile Tyr Gln Lys Val 
    770                 775                 780                 


Asp Ala Ser Glu Leu Lys Pro Tyr Thr Arg Tyr Arg Leu Asp Gly Phe 
785                 790                 795                 800 


Val Lys Ser Ser Gln Asp Leu Glu Ile Asp Leu Ile His His His Lys 
                805                 810                 815     


Val His Leu Val Lys Asn Ala Pro Asp Asn Leu Val Ser Asp Thr Tyr 
            820                 825                 830         


Pro Asp Asp Ser Cys Ser Gly Ile Asn Arg Cys Gln Glu Gln Gln Met 
        835                 840                 845             


Val Asn Ala Gln Leu Glu Thr Glu His His His Pro Met Asp Cys Cys 
    850                 855                 860                 


Gly Ala Ala Gln Thr His Glu Phe Ser Ser Tyr Ile Asp Thr Gly Asp 
865                 870                 875                 880 


Leu Asn Ser Thr Val Asp Gln Gly Ile Trp Ala Ile Phe Lys Val Arg 
                885                 890                 895     


Thr Thr Asp Gly Tyr Ala Thr Leu Gly Asn Leu Glu Leu Val Glu Ile 
            900                 905                 910         


Gly Pro Leu Ser Gly Glu Ser Leu Glu Arg Glu Gln Arg Asp Asn Ala 
        915                 920                 925             


Lys Trp Ser Ala Glu Leu Gly Arg Lys Arg Ala Glu Thr Asp Arg Val 
    930                 935                 940                 


Tyr Gln Asp Ala Lys Gln Ser Ile Asn His Leu Phe Val Asp Tyr Gln 
945                 950                 955                 960 


Asp Gln Gln Leu Asn Pro Glu Ile Gly Met Ala Asp Ile Met Asp Ala 
                965                 970                 975     


Gln Asn Leu Val Ala Ser Ile Ser Asp Val Tyr Ser Asp Ala Val Leu 
            980                 985                 990         


Gln Ile Pro Gly Ile Asn Tyr Glu  Ile Tyr Thr Glu Leu  Ser Asn Arg 
        995                 1000                 1005             


Leu Gln  Gln Ala Ser Tyr Leu  Tyr Thr Ser Arg Asn  Ala Val Gln 
    1010                 1015                 1020             


Asn Gly  Asp Phe Asn Ser Gly  Leu Asp Ser Trp Asn  Ala Thr Ala 
    1025                 1030                 1035             


Gly Ala  Thr Val Gln Gln Asp  Gly Asn Thr His Phe  Leu Val Leu 
    1040                 1045                 1050             


Ser His  Trp Asp Ala Gln Val  Ser Gln Gln Phe Arg  Val Gln Pro 
    1055                 1060                 1065             


Asn Cys  Lys Tyr Val Leu Arg  Val Thr Ala Glu Lys  Val Gly Gly 
    1070                 1075                 1080             


Gly Asp  Gly Tyr Val Thr Ile  Arg Asp Gly Ala His  His Thr Glu 
    1085                 1090                 1095             


Thr Leu  Thr Phe Asn Ala Cys  Asp Tyr Asp Ile Asn  Gly Thr Tyr 
    1100                 1105                 1110             


Val Thr  Asp Asn Thr Tyr Leu  Thr Lys Glu Val Val  Phe Tyr Ser 
    1115                 1120                 1125             


His Thr  Glu His Met Trp Val  Glu Val Ser Glu Thr  Glu Gly Val 
    1130                 1135                 1140             


Phe His  Ile Asp Ser Val Glu  Phe Met Glu Thr Gln  Gln 
    1145                 1150                 1155     


<210>  49
<211>  3471
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic bacterial coding sequence encoding TIC4064_10 
       comprising a mutation at S94T.

<400>  49
atgaatcaaa ataaacacgg aattattggc gcttccaatt gtggttgtac gtcagataat       60

gttgcgaaat atcctttagc caacaatcca tattcatctg ctttaaattt aaattcttgt      120

caaaatagta gtattctcaa ctggattaac ataataggcg atgcagcaaa agaagcagta      180

tctattggga caacaatagt ctctcttatc acagcacctt ctcttactgg attaatttca      240

atagtatatg accttatagg taaagtacta ggaggtagta caggacaatc catatcagat      300

ttgtctatat gtgacttatt atctattatt gatttacggg taagtcagag tgttttaaat      360

gatgggattg cagattttaa tggttctgta ctcttataca ggaactattt agaggctctg      420

gatagctgga ataagaatcc taattctgct tctgctgaag aactccgtac tcgttttaga      480

atcgctgact cagaatttga tagaatttta acacgagggt ctttaacgaa tggtggctcg      540

ttagctagac aaaatgccca aatattatta ttaccttctt ttgcgagtgc tgcatttttc      600

catttattac tactaaggga tgctactaga tatggcacta attgggggct atacaatgct      660

acacctttta taaattatca atcaaaacta gtagagctta ttgaactata tactgattat      720

tgcgtacatt ggtataatcg aggtttcaac gaactaaggc aacgaggcac tagtgctaca      780

gcttggttag aatttcatag atatcgtaga gagatgacat tgatggtatt agatatagta      840

gcatcatttt caagtcttga tattactaat tacccaatag aaacagattt tcagttgagt      900

agggtcattt atacagatcc aattggtttt gtacatcgta gtagtcttag gggagaaagt      960

tggtttagct ttgttaatag agctaatttc tcagatttag aaaatgcaat acctaatcct     1020

agaccgtctt ggtttttaaa taatatgatt atatctactg gttcacttac attgccggtt     1080

agcccaagta ctgatagagc gagggtatgg tatggaagtc gagatcgaat ttcccctgct     1140

aattcacaat ttattactga actaatctct ggacaacata cgactgctac acaaactatt     1200

ttagggcgaa atatatttag agtagattct caagcttgta atttaaatga taccacatat     1260

ggagtgaata gggcggtatt ttatcatgat gcgagtgaag gttctcaaag atccgtgtac     1320

gaggggtata ttcgaacaac tgggatagat aaccctagag ttcaaaatat taacacttat     1380

ttacctggag aaaattcaga tatcccaact ccagaagact atactcatat attaagcaca     1440

acaataaatt taacaggagg acttagacaa gtagcatcta atcgccgttc atctttagta     1500

atgtatggtt ggacacataa aagtctggct cgtaacaata ccattaatcc agatagaatt     1560

acacagatac cattgacgaa ggttgatacc cgaggcacag gtgtttctta tgtgaatgat     1620

ccaggattta taggaggagc tctacttcaa aggactgacc atggttcgct tggagtattg     1680

agggtccaat ttccacttca cttaagacaa caatatcgca ttagagtccg ttatgcttct     1740

acaacaaata ttcgattgag tgtgaatggc agtttcggta ctatttctca aaatctccct     1800

agtacaatga gattaggaga ggatttaaga tacggatctt ttgctataag agagtttagt     1860

acttctatta gacccactgc aagtccggac caaattcgat tgacaataga accatctttt     1920

attagacaag aggtctatgt agatagaatt gagttcattc cagttaatcc gacgcgagag     1980

gcgaaagagg atctagaagc aacaaagaaa gcggtggcga gcttgtttac acgcacaagg     2040

gacggattac aagtaaatgt gacagattat caagtcgatc aagcggcaaa tttagtgtca     2100

tgcttatcag atgaacaata tgcgcatgat aaaaagatgt tattggaagc ggtacgcgcg     2160

gcaaaacgcc tcagccgaga acgcaactta cttcaggatc cagattttaa tacaatcaat     2220

agtacagaag aaaatggatg gaaagcaagt aacggcgtta ctattagcga aggcggtcca     2280

ttctataaag gccgtgcact tcagctagca agtgcacgag aaaattaccc aacatacatc     2340

tatcaaaaag tagatgcatc ggagttaaag ccgtatacac gttatagact ggatgggttc     2400

gtgaagagta gtcaagattt agaaattgat ctcattcacc atcataaagt ccatcttgtg     2460

aaaaatgcac cagataattt agtatctgat acttacccag atgattcttg tagtggaatc     2520

aatcgatgtc aggaacaaca gatggtaaat gcgcaactgg aaacagaaca tcatcatccg     2580

atggattgct gtggagcagc tcaaacacat gagttttctt cctatattga tacaggggat     2640

ttaaattcga ctgtagacca gggaatctgg gcgatcttta aagttcgaac aacagatggt     2700

tatgcgacgt taggaaatct tgaattggta gagatcggac cgttatcggg tgaatctcta     2760

gaacgtgaac aaagggataa tgcaaaatgg agtgcagagc taggaagaaa gcgtgcagaa     2820

acagatcgcg tgtatcaaga tgccaaacaa tccatcaatc atttatttgt ggattatcaa     2880

gatcaacaat taaatccaga aatagggatg gcagatatta tggacgctca aaatcttgtc     2940

gcatcaattt cagatgtata tagcgatgca gtactgcaaa tccctggaat taactatgag     3000

atttacacag agctatccaa tcgcttacaa caagcatcgt atctgtatac gtctcgaaat     3060

gcggtgcaaa atggggactt taacagcggt ctagatagtt ggaatgcaac agcgggtgct     3120

acggtacaac aggatggtaa tacgcatttc ttagttcttt ctcattggga tgcacaagtt     3180

tctcaacaat ttagagtgca gccaaattgt aaatatgtat tacgtgtaac agcagagaaa     3240

gtaggcggcg gagacggata cgtgacaatc cgggatggtg ctcatcatac agaaacgctt     3300

acatttaatg catgtgatta tgatataaat ggcacgtacg tgactgataa tacgtatcta     3360

acaaaagaag tggtattcta ttcacataca gaacacatgt gggtagaggt aagtgaaaca     3420

gaaggtgttt tccatataga cagtgttgag ttcatggaaa cccaacagta g              3471


<210>  50
<211>  1156
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of TIC4064_10 comprising a mutation at S94T.

<400>  50

Met Asn Gln Asn Lys His Gly Ile Ile Gly Ala Ser Asn Cys Gly Cys 
1               5                   10                  15      


Thr Ser Asp Asn Val Ala Lys Tyr Pro Leu Ala Asn Asn Pro Tyr Ser 
            20                  25                  30          


Ser Ala Leu Asn Leu Asn Ser Cys Gln Asn Ser Ser Ile Leu Asn Trp 
        35                  40                  45              


Ile Asn Ile Ile Gly Asp Ala Ala Lys Glu Ala Val Ser Ile Gly Thr 
    50                  55                  60                  


Thr Ile Val Ser Leu Ile Thr Ala Pro Ser Leu Thr Gly Leu Ile Ser 
65                  70                  75                  80  


Ile Val Tyr Asp Leu Ile Gly Lys Val Leu Gly Gly Ser Thr Gly Gln 
                85                  90                  95      


Ser Ile Ser Asp Leu Ser Ile Cys Asp Leu Leu Ser Ile Ile Asp Leu 
            100                 105                 110         


Arg Val Ser Gln Ser Val Leu Asn Asp Gly Ile Ala Asp Phe Asn Gly 
        115                 120                 125             


Ser Val Leu Leu Tyr Arg Asn Tyr Leu Glu Ala Leu Asp Ser Trp Asn 
    130                 135                 140                 


Lys Asn Pro Asn Ser Ala Ser Ala Glu Glu Leu Arg Thr Arg Phe Arg 
145                 150                 155                 160 


Ile Ala Asp Ser Glu Phe Asp Arg Ile Leu Thr Arg Gly Ser Leu Thr 
                165                 170                 175     


Asn Gly Gly Ser Leu Ala Arg Gln Asn Ala Gln Ile Leu Leu Leu Pro 
            180                 185                 190         


Ser Phe Ala Ser Ala Ala Phe Phe His Leu Leu Leu Leu Arg Asp Ala 
        195                 200                 205             


Thr Arg Tyr Gly Thr Asn Trp Gly Leu Tyr Asn Ala Thr Pro Phe Ile 
    210                 215                 220                 


Asn Tyr Gln Ser Lys Leu Val Glu Leu Ile Glu Leu Tyr Thr Asp Tyr 
225                 230                 235                 240 


Cys Val His Trp Tyr Asn Arg Gly Phe Asn Glu Leu Arg Gln Arg Gly 
                245                 250                 255     


Thr Ser Ala Thr Ala Trp Leu Glu Phe His Arg Tyr Arg Arg Glu Met 
            260                 265                 270         


Thr Leu Met Val Leu Asp Ile Val Ala Ser Phe Ser Ser Leu Asp Ile 
        275                 280                 285             


Thr Asn Tyr Pro Ile Glu Thr Asp Phe Gln Leu Ser Arg Val Ile Tyr 
    290                 295                 300                 


Thr Asp Pro Ile Gly Phe Val His Arg Ser Ser Leu Arg Gly Glu Ser 
305                 310                 315                 320 


Trp Phe Ser Phe Val Asn Arg Ala Asn Phe Ser Asp Leu Glu Asn Ala 
                325                 330                 335     


Ile Pro Asn Pro Arg Pro Ser Trp Phe Leu Asn Asn Met Ile Ile Ser 
            340                 345                 350         


Thr Gly Ser Leu Thr Leu Pro Val Ser Pro Ser Thr Asp Arg Ala Arg 
        355                 360                 365             


Val Trp Tyr Gly Ser Arg Asp Arg Ile Ser Pro Ala Asn Ser Gln Phe 
    370                 375                 380                 


Ile Thr Glu Leu Ile Ser Gly Gln His Thr Thr Ala Thr Gln Thr Ile 
385                 390                 395                 400 


Leu Gly Arg Asn Ile Phe Arg Val Asp Ser Gln Ala Cys Asn Leu Asn 
                405                 410                 415     


Asp Thr Thr Tyr Gly Val Asn Arg Ala Val Phe Tyr His Asp Ala Ser 
            420                 425                 430         


Glu Gly Ser Gln Arg Ser Val Tyr Glu Gly Tyr Ile Arg Thr Thr Gly 
        435                 440                 445             


Ile Asp Asn Pro Arg Val Gln Asn Ile Asn Thr Tyr Leu Pro Gly Glu 
    450                 455                 460                 


Asn Ser Asp Ile Pro Thr Pro Glu Asp Tyr Thr His Ile Leu Ser Thr 
465                 470                 475                 480 


Thr Ile Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Ser Asn Arg Arg 
                485                 490                 495     


Ser Ser Leu Val Met Tyr Gly Trp Thr His Lys Ser Leu Ala Arg Asn 
            500                 505                 510         


Asn Thr Ile Asn Pro Asp Arg Ile Thr Gln Ile Pro Leu Thr Lys Val 
        515                 520                 525             


Asp Thr Arg Gly Thr Gly Val Ser Tyr Val Asn Asp Pro Gly Phe Ile 
    530                 535                 540                 


Gly Gly Ala Leu Leu Gln Arg Thr Asp His Gly Ser Leu Gly Val Leu 
545                 550                 555                 560 


Arg Val Gln Phe Pro Leu His Leu Arg Gln Gln Tyr Arg Ile Arg Val 
                565                 570                 575     


Arg Tyr Ala Ser Thr Thr Asn Ile Arg Leu Ser Val Asn Gly Ser Phe 
            580                 585                 590         


Gly Thr Ile Ser Gln Asn Leu Pro Ser Thr Met Arg Leu Gly Glu Asp 
        595                 600                 605             


Leu Arg Tyr Gly Ser Phe Ala Ile Arg Glu Phe Ser Thr Ser Ile Arg 
    610                 615                 620                 


Pro Thr Ala Ser Pro Asp Gln Ile Arg Leu Thr Ile Glu Pro Ser Phe 
625                 630                 635                 640 


Ile Arg Gln Glu Val Tyr Val Asp Arg Ile Glu Phe Ile Pro Val Asn 
                645                 650                 655     


Pro Thr Arg Glu Ala Lys Glu Asp Leu Glu Ala Thr Lys Lys Ala Val 
            660                 665                 670         


Ala Ser Leu Phe Thr Arg Thr Arg Asp Gly Leu Gln Val Asn Val Thr 
        675                 680                 685             


Asp Tyr Gln Val Asp Gln Ala Ala Asn Leu Val Ser Cys Leu Ser Asp 
    690                 695                 700                 


Glu Gln Tyr Ala His Asp Lys Lys Met Leu Leu Glu Ala Val Arg Ala 
705                 710                 715                 720 


Ala Lys Arg Leu Ser Arg Glu Arg Asn Leu Leu Gln Asp Pro Asp Phe 
                725                 730                 735     


Asn Thr Ile Asn Ser Thr Glu Glu Asn Gly Trp Lys Ala Ser Asn Gly 
            740                 745                 750         


Val Thr Ile Ser Glu Gly Gly Pro Phe Tyr Lys Gly Arg Ala Leu Gln 
        755                 760                 765             


Leu Ala Ser Ala Arg Glu Asn Tyr Pro Thr Tyr Ile Tyr Gln Lys Val 
    770                 775                 780                 


Asp Ala Ser Glu Leu Lys Pro Tyr Thr Arg Tyr Arg Leu Asp Gly Phe 
785                 790                 795                 800 


Val Lys Ser Ser Gln Asp Leu Glu Ile Asp Leu Ile His His His Lys 
                805                 810                 815     


Val His Leu Val Lys Asn Ala Pro Asp Asn Leu Val Ser Asp Thr Tyr 
            820                 825                 830         


Pro Asp Asp Ser Cys Ser Gly Ile Asn Arg Cys Gln Glu Gln Gln Met 
        835                 840                 845             


Val Asn Ala Gln Leu Glu Thr Glu His His His Pro Met Asp Cys Cys 
    850                 855                 860                 


Gly Ala Ala Gln Thr His Glu Phe Ser Ser Tyr Ile Asp Thr Gly Asp 
865                 870                 875                 880 


Leu Asn Ser Thr Val Asp Gln Gly Ile Trp Ala Ile Phe Lys Val Arg 
                885                 890                 895     


Thr Thr Asp Gly Tyr Ala Thr Leu Gly Asn Leu Glu Leu Val Glu Ile 
            900                 905                 910         


Gly Pro Leu Ser Gly Glu Ser Leu Glu Arg Glu Gln Arg Asp Asn Ala 
        915                 920                 925             


Lys Trp Ser Ala Glu Leu Gly Arg Lys Arg Ala Glu Thr Asp Arg Val 
    930                 935                 940                 


Tyr Gln Asp Ala Lys Gln Ser Ile Asn His Leu Phe Val Asp Tyr Gln 
945                 950                 955                 960 


Asp Gln Gln Leu Asn Pro Glu Ile Gly Met Ala Asp Ile Met Asp Ala 
                965                 970                 975     


Gln Asn Leu Val Ala Ser Ile Ser Asp Val Tyr Ser Asp Ala Val Leu 
            980                 985                 990         


Gln Ile Pro Gly Ile Asn Tyr Glu  Ile Tyr Thr Glu Leu  Ser Asn Arg 
        995                 1000                 1005             


Leu Gln  Gln Ala Ser Tyr Leu  Tyr Thr Ser Arg Asn  Ala Val Gln 
    1010                 1015                 1020             


Asn Gly  Asp Phe Asn Ser Gly  Leu Asp Ser Trp Asn  Ala Thr Ala 
    1025                 1030                 1035             


Gly Ala  Thr Val Gln Gln Asp  Gly Asn Thr His Phe  Leu Val Leu 
    1040                 1045                 1050             


Ser His  Trp Asp Ala Gln Val  Ser Gln Gln Phe Arg  Val Gln Pro 
    1055                 1060                 1065             


Asn Cys  Lys Tyr Val Leu Arg  Val Thr Ala Glu Lys  Val Gly Gly 
    1070                 1075                 1080             


Gly Asp  Gly Tyr Val Thr Ile  Arg Asp Gly Ala His  His Thr Glu 
    1085                 1090                 1095             


Thr Leu  Thr Phe Asn Ala Cys  Asp Tyr Asp Ile Asn  Gly Thr Tyr 
    1100                 1105                 1110             


Val Thr  Asp Asn Thr Tyr Leu  Thr Lys Glu Val Val  Phe Tyr Ser 
    1115                 1120                 1125             


His Thr  Glu His Met Trp Val  Glu Val Ser Glu Thr  Glu Gly Val 
    1130                 1135                 1140             


Phe His  Ile Asp Ser Val Glu  Phe Met Glu Thr Gln  Gln 
    1145                 1150                 1155     


<210>  51
<211>  3471
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic bacterial coding sequence encoding TIC4064_11 
       comprising a mutation at G87K.

<400>  51
atgaatcaaa ataaacacgg aattattggc gcttccaatt gtggttgtac gtcagataat       60

gttgcgaaat atcctttagc caacaatcca tattcatctg ctttaaattt aaattcttgt      120

caaaatagta gtattctcaa ctggattaac ataataggcg atgcagcaaa agaagcagta      180

tctattggga caacaatagt ctctcttatc acagcacctt ctcttactgg attaatttca      240

atagtatatg accttataaa aaaagtacta ggaggtagta gtggacaatc catatcagat      300

ttgtctatat gtgacttatt atctattatt gatttacggg taagtcagag tgttttaaat      360

gatgggattg cagattttaa tggttctgta ctcttataca ggaactattt agaggctctg      420

gatagctgga ataagaatcc taattctgct tctgctgaag aactccgtac tcgttttaga      480

atcgctgact cagaatttga tagaatttta acacgagggt ctttaacgaa tggtggctcg      540

ttagctagac aaaatgccca aatattatta ttaccttctt ttgcgagtgc tgcatttttc      600

catttattac tactaaggga tgctactaga tatggcacta attgggggct atacaatgct      660

acacctttta taaattatca atcaaaacta gtagagctta ttgaactata tactgattat      720

tgcgtacatt ggtataatcg aggtttcaac gaactaaggc aacgaggcac tagtgctaca      780

gcttggttag aatttcatag atatcgtaga gagatgacat tgatggtatt agatatagta      840

gcatcatttt caagtcttga tattactaat tacccaatag aaacagattt tcagttgagt      900

agggtcattt atacagatcc aattggtttt gtacatcgta gtagtcttag gggagaaagt      960

tggtttagct ttgttaatag agctaatttc tcagatttag aaaatgcaat acctaatcct     1020

agaccgtctt ggtttttaaa taatatgatt atatctactg gttcacttac attgccggtt     1080

agcccaagta ctgatagagc gagggtatgg tatggaagtc gagatcgaat ttcccctgct     1140

aattcacaat ttattactga actaatctct ggacaacata cgactgctac acaaactatt     1200

ttagggcgaa atatatttag agtagattct caagcttgta atttaaatga taccacatat     1260

ggagtgaata gggcggtatt ttatcatgat gcgagtgaag gttctcaaag atccgtgtac     1320

gaggggtata ttcgaacaac tgggatagat aaccctagag ttcaaaatat taacacttat     1380

ttacctggag aaaattcaga tatcccaact ccagaagact atactcatat attaagcaca     1440

acaataaatt taacaggagg acttagacaa gtagcatcta atcgccgttc atctttagta     1500

atgtatggtt ggacacataa aagtctggct cgtaacaata ccattaatcc agatagaatt     1560

acacagatac cattgacgaa ggttgatacc cgaggcacag gtgtttctta tgtgaatgat     1620

ccaggattta taggaggagc tctacttcaa aggactgacc atggttcgct tggagtattg     1680

agggtccaat ttccacttca cttaagacaa caatatcgca ttagagtccg ttatgcttct     1740

acaacaaata ttcgattgag tgtgaatggc agtttcggta ctatttctca aaatctccct     1800

agtacaatga gattaggaga ggatttaaga tacggatctt ttgctataag agagtttagt     1860

acttctatta gacccactgc aagtccggac caaattcgat tgacaataga accatctttt     1920

attagacaag aggtctatgt agatagaatt gagttcattc cagttaatcc gacgcgagag     1980

gcgaaagagg atctagaagc aacaaagaaa gcggtggcga gcttgtttac acgcacaagg     2040

gacggattac aagtaaatgt gacagattat caagtcgatc aagcggcaaa tttagtgtca     2100

tgcttatcag atgaacaata tgcgcatgat aaaaagatgt tattggaagc ggtacgcgcg     2160

gcaaaacgcc tcagccgaga acgcaactta cttcaggatc cagattttaa tacaatcaat     2220

agtacagaag aaaatggatg gaaagcaagt aacggcgtta ctattagcga aggcggtcca     2280

ttctataaag gccgtgcact tcagctagca agtgcacgag aaaattaccc aacatacatc     2340

tatcaaaaag tagatgcatc ggagttaaag ccgtatacac gttatagact ggatgggttc     2400

gtgaagagta gtcaagattt agaaattgat ctcattcacc atcataaagt ccatcttgtg     2460

aaaaatgcac cagataattt agtatctgat acttacccag atgattcttg tagtggaatc     2520

aatcgatgtc aggaacaaca gatggtaaat gcgcaactgg aaacagaaca tcatcatccg     2580

atggattgct gtggagcagc tcaaacacat gagttttctt cctatattga tacaggggat     2640

ttaaattcga ctgtagacca gggaatctgg gcgatcttta aagttcgaac aacagatggt     2700

tatgcgacgt taggaaatct tgaattggta gagatcggac cgttatcggg tgaatctcta     2760

gaacgtgaac aaagggataa tgcaaaatgg agtgcagagc taggaagaaa gcgtgcagaa     2820

acagatcgcg tgtatcaaga tgccaaacaa tccatcaatc atttatttgt ggattatcaa     2880

gatcaacaat taaatccaga aatagggatg gcagatatta tggacgctca aaatcttgtc     2940

gcatcaattt cagatgtata tagcgatgca gtactgcaaa tccctggaat taactatgag     3000

atttacacag agctatccaa tcgcttacaa caagcatcgt atctgtatac gtctcgaaat     3060

gcggtgcaaa atggggactt taacagcggt ctagatagtt ggaatgcaac agcgggtgct     3120

acggtacaac aggatggtaa tacgcatttc ttagttcttt ctcattggga tgcacaagtt     3180

tctcaacaat ttagagtgca gccaaattgt aaatatgtat tacgtgtaac agcagagaaa     3240

gtaggcggcg gagacggata cgtgacaatc cgggatggtg ctcatcatac agaaacgctt     3300

acatttaatg catgtgatta tgatataaat ggcacgtacg tgactgataa tacgtatcta     3360

acaaaagaag tggtattcta ttcacataca gaacacatgt gggtagaggt aagtgaaaca     3420

gaaggtgttt tccatataga cagtgttgag ttcatggaaa cccaacagta g              3471


<210>  52
<211>  1156
<212>  PRT
<213>  Artificial

<220>
<223>  Amino acid sequence of TIC4064_11 comprising a mutation at G87K.

<400>  52

Met Asn Gln Asn Lys His Gly Ile Ile Gly Ala Ser Asn Cys Gly Cys 
1               5                   10                  15      


Thr Ser Asp Asn Val Ala Lys Tyr Pro Leu Ala Asn Asn Pro Tyr Ser 
            20                  25                  30          


Ser Ala Leu Asn Leu Asn Ser Cys Gln Asn Ser Ser Ile Leu Asn Trp 
        35                  40                  45              


Ile Asn Ile Ile Gly Asp Ala Ala Lys Glu Ala Val Ser Ile Gly Thr 
    50                  55                  60                  


Thr Ile Val Ser Leu Ile Thr Ala Pro Ser Leu Thr Gly Leu Ile Ser 
65                  70                  75                  80  


Ile Val Tyr Asp Leu Ile Lys Lys Val Leu Gly Gly Ser Ser Gly Gln 
                85                  90                  95      


Ser Ile Ser Asp Leu Ser Ile Cys Asp Leu Leu Ser Ile Ile Asp Leu 
            100                 105                 110         


Arg Val Ser Gln Ser Val Leu Asn Asp Gly Ile Ala Asp Phe Asn Gly 
        115                 120                 125             


Ser Val Leu Leu Tyr Arg Asn Tyr Leu Glu Ala Leu Asp Ser Trp Asn 
    130                 135                 140                 


Lys Asn Pro Asn Ser Ala Ser Ala Glu Glu Leu Arg Thr Arg Phe Arg 
145                 150                 155                 160 


Ile Ala Asp Ser Glu Phe Asp Arg Ile Leu Thr Arg Gly Ser Leu Thr 
                165                 170                 175     


Asn Gly Gly Ser Leu Ala Arg Gln Asn Ala Gln Ile Leu Leu Leu Pro 
            180                 185                 190         


Ser Phe Ala Ser Ala Ala Phe Phe His Leu Leu Leu Leu Arg Asp Ala 
        195                 200                 205             


Thr Arg Tyr Gly Thr Asn Trp Gly Leu Tyr Asn Ala Thr Pro Phe Ile 
    210                 215                 220                 


Asn Tyr Gln Ser Lys Leu Val Glu Leu Ile Glu Leu Tyr Thr Asp Tyr 
225                 230                 235                 240 


Cys Val His Trp Tyr Asn Arg Gly Phe Asn Glu Leu Arg Gln Arg Gly 
                245                 250                 255     


Thr Ser Ala Thr Ala Trp Leu Glu Phe His Arg Tyr Arg Arg Glu Met 
            260                 265                 270         


Thr Leu Met Val Leu Asp Ile Val Ala Ser Phe Ser Ser Leu Asp Ile 
        275                 280                 285             


Thr Asn Tyr Pro Ile Glu Thr Asp Phe Gln Leu Ser Arg Val Ile Tyr 
    290                 295                 300                 


Thr Asp Pro Ile Gly Phe Val His Arg Ser Ser Leu Arg Gly Glu Ser 
305                 310                 315                 320 


Trp Phe Ser Phe Val Asn Arg Ala Asn Phe Ser Asp Leu Glu Asn Ala 
                325                 330                 335     


Ile Pro Asn Pro Arg Pro Ser Trp Phe Leu Asn Asn Met Ile Ile Ser 
            340                 345                 350         


Thr Gly Ser Leu Thr Leu Pro Val Ser Pro Ser Thr Asp Arg Ala Arg 
        355                 360                 365             


Val Trp Tyr Gly Ser Arg Asp Arg Ile Ser Pro Ala Asn Ser Gln Phe 
    370                 375                 380                 


Ile Thr Glu Leu Ile Ser Gly Gln His Thr Thr Ala Thr Gln Thr Ile 
385                 390                 395                 400 


Leu Gly Arg Asn Ile Phe Arg Val Asp Ser Gln Ala Cys Asn Leu Asn 
                405                 410                 415     


Asp Thr Thr Tyr Gly Val Asn Arg Ala Val Phe Tyr His Asp Ala Ser 
            420                 425                 430         


Glu Gly Ser Gln Arg Ser Val Tyr Glu Gly Tyr Ile Arg Thr Thr Gly 
        435                 440                 445             


Ile Asp Asn Pro Arg Val Gln Asn Ile Asn Thr Tyr Leu Pro Gly Glu 
    450                 455                 460                 


Asn Ser Asp Ile Pro Thr Pro Glu Asp Tyr Thr His Ile Leu Ser Thr 
465                 470                 475                 480 


Thr Ile Asn Leu Thr Gly Gly Leu Arg Gln Val Ala Ser Asn Arg Arg 
                485                 490                 495     


Ser Ser Leu Val Met Tyr Gly Trp Thr His Lys Ser Leu Ala Arg Asn 
            500                 505                 510         


Asn Thr Ile Asn Pro Asp Arg Ile Thr Gln Ile Pro Leu Thr Lys Val 
        515                 520                 525             


Asp Thr Arg Gly Thr Gly Val Ser Tyr Val Asn Asp Pro Gly Phe Ile 
    530                 535                 540                 


Gly Gly Ala Leu Leu Gln Arg Thr Asp His Gly Ser Leu Gly Val Leu 
545                 550                 555                 560 


Arg Val Gln Phe Pro Leu His Leu Arg Gln Gln Tyr Arg Ile Arg Val 
                565                 570                 575     


Arg Tyr Ala Ser Thr Thr Asn Ile Arg Leu Ser Val Asn Gly Ser Phe 
            580                 585                 590         


Gly Thr Ile Ser Gln Asn Leu Pro Ser Thr Met Arg Leu Gly Glu Asp 
        595                 600                 605             


Leu Arg Tyr Gly Ser Phe Ala Ile Arg Glu Phe Ser Thr Ser Ile Arg 
    610                 615                 620                 


Pro Thr Ala Ser Pro Asp Gln Ile Arg Leu Thr Ile Glu Pro Ser Phe 
625                 630                 635                 640 


Ile Arg Gln Glu Val Tyr Val Asp Arg Ile Glu Phe Ile Pro Val Asn 
                645                 650                 655     


Pro Thr Arg Glu Ala Lys Glu Asp Leu Glu Ala Thr Lys Lys Ala Val 
            660                 665                 670         


Ala Ser Leu Phe Thr Arg Thr Arg Asp Gly Leu Gln Val Asn Val Thr 
        675                 680                 685             


Asp Tyr Gln Val Asp Gln Ala Ala Asn Leu Val Ser Cys Leu Ser Asp 
    690                 695                 700                 


Glu Gln Tyr Ala His Asp Lys Lys Met Leu Leu Glu Ala Val Arg Ala 
705                 710                 715                 720 


Ala Lys Arg Leu Ser Arg Glu Arg Asn Leu Leu Gln Asp Pro Asp Phe 
                725                 730                 735     


Asn Thr Ile Asn Ser Thr Glu Glu Asn Gly Trp Lys Ala Ser Asn Gly 
            740                 745                 750         


Val Thr Ile Ser Glu Gly Gly Pro Phe Tyr Lys Gly Arg Ala Leu Gln 
        755                 760                 765             


Leu Ala Ser Ala Arg Glu Asn Tyr Pro Thr Tyr Ile Tyr Gln Lys Val 
    770                 775                 780                 


Asp Ala Ser Glu Leu Lys Pro Tyr Thr Arg Tyr Arg Leu Asp Gly Phe 
785                 790                 795                 800 


Val Lys Ser Ser Gln Asp Leu Glu Ile Asp Leu Ile His His His Lys 
                805                 810                 815     


Val His Leu Val Lys Asn Ala Pro Asp Asn Leu Val Ser Asp Thr Tyr 
            820                 825                 830         


Pro Asp Asp Ser Cys Ser Gly Ile Asn Arg Cys Gln Glu Gln Gln Met 
        835                 840                 845             


Val Asn Ala Gln Leu Glu Thr Glu His His His Pro Met Asp Cys Cys 
    850                 855                 860                 


Gly Ala Ala Gln Thr His Glu Phe Ser Ser Tyr Ile Asp Thr Gly Asp 
865                 870                 875                 880 


Leu Asn Ser Thr Val Asp Gln Gly Ile Trp Ala Ile Phe Lys Val Arg 
                885                 890                 895     


Thr Thr Asp Gly Tyr Ala Thr Leu Gly Asn Leu Glu Leu Val Glu Ile 
            900                 905                 910         


Gly Pro Leu Ser Gly Glu Ser Leu Glu Arg Glu Gln Arg Asp Asn Ala 
        915                 920                 925             


Lys Trp Ser Ala Glu Leu Gly Arg Lys Arg Ala Glu Thr Asp Arg Val 
    930                 935                 940                 


Tyr Gln Asp Ala Lys Gln Ser Ile Asn His Leu Phe Val Asp Tyr Gln 
945                 950                 955                 960 


Asp Gln Gln Leu Asn Pro Glu Ile Gly Met Ala Asp Ile Met Asp Ala 
                965                 970                 975     


Gln Asn Leu Val Ala Ser Ile Ser Asp Val Tyr Ser Asp Ala Val Leu 
            980                 985                 990         


Gln Ile Pro Gly Ile Asn Tyr Glu  Ile Tyr Thr Glu Leu  Ser Asn Arg 
        995                 1000                 1005             


Leu Gln  Gln Ala Ser Tyr Leu  Tyr Thr Ser Arg Asn  Ala Val Gln 
    1010                 1015                 1020             


Asn Gly  Asp Phe Asn Ser Gly  Leu Asp Ser Trp Asn  Ala Thr Ala 
    1025                 1030                 1035             


Gly Ala  Thr Val Gln Gln Asp  Gly Asn Thr His Phe  Leu Val Leu 
    1040                 1045                 1050             


Ser His  Trp Asp Ala Gln Val  Ser Gln Gln Phe Arg  Val Gln Pro 
    1055                 1060                 1065             


Asn Cys  Lys Tyr Val Leu Arg  Val Thr Ala Glu Lys  Val Gly Gly 
    1070                 1075                 1080             


Gly Asp  Gly Tyr Val Thr Ile  Arg Asp Gly Ala His  His Thr Glu 
    1085                 1090                 1095             


Thr Leu  Thr Phe Asn Ala Cys  Asp Tyr Asp Ile Asn  Gly Thr Tyr 
    1100                 1105                 1110             


Val Thr  Asp Asn Thr Tyr Leu  Thr Lys Glu Val Val  Phe Tyr Ser 
    1115                 1120                 1125             


His Thr  Glu His Met Trp Val  Glu Val Ser Glu Thr  Glu Gly Val 
    1130                 1135                 1140             


Phe His  Ile Asp Ser Val Glu  Phe Met Glu Thr Gln  Gln 
    1145                 1150                 1155     


