                               SEQUENCE LISTING

<110> ALAUNUS BIOSCIENCES, INC.
 
<120> PEPTIDASE CLEAVABLE SUBSTRATES AND METHODS OF IDENTIFICATION AND
      USE THEREOF

<130> 059152-501001WO

<140> PCT/US2021/064623
<141> 2021-12-21

<150> 63/128,475
<151> 2020-12-21

<160> 52    

<170> PatentIn version 3.5

<210> 1
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (2)..(2)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (6)..(6)
<223> M or L

<220>
<221> MOD_RES
<222> (8)..(8)
<223> G or A

<400> 1
Met Xaa Pro Leu Gly Xaa Met Xaa Arg 
1               5                   


<210> 2
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 2
Pro Leu Gly Leu Tyr 
1               5   


<210> 3
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 3
Pro His Gly Leu Lys 
1               5   


<210> 4
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 4
Pro Leu Gly Leu Leu 
1               5   


<210> 5
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 5
Pro Gln Gly Leu Lys 
1               5   


<210> 6
<211> 8
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (3)..(3)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (5)..(5)
<223> Any small flexible amino acid

<220>
<223> See specification as filed for detailed description of
      substitutions and preferred embodiments

<400> 6
Pro Ala Xaa Leu Xaa Gly Arg Leu 
1               5               


<210> 7
<211> 8
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (5)..(5)
<223> I or F

<220>
<221> MOD_RES
<222> (7)..(7)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (8)..(8)
<223> H or N

<400> 7
Tyr Pro Thr Leu Xaa Tyr Xaa Xaa 
1               5               


<210> 8
<211> 8
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 8
Lys Arg Ala Leu Gly Leu Pro Gly 
1               5               


<210> 9
<211> 8
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 9
Arg Pro Leu Ala Leu Trp Arg Ser 
1               5               


<210> 10
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 10
Leu Glu Ala Thr Ala 
1               5   


<210> 11
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 11
Gly Gly Ala Ala Asn Leu Val Arg Gly Gly 
1               5                   10  


<210> 12
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 12
Ser Gly Arg Ile Gly Phe Leu Arg Thr Ala 
1               5                   10  


<210> 13
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 13
Pro Leu Gly Leu Ala Gly 
1               5       


<210> 14
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (6)..(6)
<223> Any amino acid

<400> 14
Pro Leu Gly Leu Ala Xaa 
1               5       


<210> 15
<211> 8
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 15
Glu Ser Pro Ala Tyr Tyr Thr Ala 
1               5               


<210> 16
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 16
Arg Leu Gln Leu Lys Leu 
1               5       


<210> 17
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 17
Arg Leu Gln Leu Lys Ala Cys 
1               5           


<210> 18
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (3)..(3)
<223> Citrulline

<220>
<221> MOD_RES
<222> (5)..(5)
<223> Homophenylalanine

<400> 18
Glu Pro Xaa Gly Phe Tyr Leu 
1               5           


<210> 19
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 19
Ser Gly Arg Ser Ala 
1               5   


<210> 20
<211> 4
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 20
Asp Ala Phe Lys 
1               


<210> 21
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 21
Gly Gly Gly Arg Arg 
1               5   


<210> 22
<211> 4
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 22
Gly Phe Leu Gly 
1               


<210> 23
<211> 4
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (1)..(1)
<223> Beta-alanine

<400> 23
Ala Leu Ala Leu 
1               


<210> 24
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 24
Pro Ile Cys Phe Phe 
1               5   


<210> 25
<211> 8
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 25
Gly Gly Pro Arg Gly Leu Pro Gly 
1               5               


<210> 26
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 26
His Ser Ser Lys Leu Gln 
1               5       


<210> 27
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 27
His Ser Ser Lys Leu Gln Leu 
1               5           


<210> 28
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 28
His Ser Ser Lys Leu Gln Glu Asp Ala 
1               5                   


<210> 29
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 29
Leu Val Leu Ala Ser Ser Ser Phe Gly Tyr 
1               5                   10  


<210> 30
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 30
Gly Val Ser Gln Asn Tyr Pro Ile Val Gly 
1               5                   10  


<210> 31
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 31
Gly Val Val Gln Ala Ser Cys Arg Leu Ala 
1               5                   10  


<210> 32
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 32
Asp Pro Arg Ser Phe Leu 
1               5       


<210> 33
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 33
Pro Pro Arg Ser Phe Leu 
1               5       


<210> 34
<211> 4
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 34
Asp Glu Val Asp 
1               


<210> 35
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 35
Asp Glu Val Asp Pro 
1               5   


<210> 36
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 36
Gly Asp Glu Val Asp Gly Ser Gly Lys 
1               5                   


<210> 37
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 37
Gly Trp Glu His Asp Gly 
1               5       


<210> 38
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 38
Glu Asp Asp Asp Asp Lys Ala 
1               5           


<210> 39
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 39
Thr Ser Gly Pro Asn Gln Glu Gln Lys 
1               5                   


<210> 40
<211> 8
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 40
Leu Ser Gly Arg Ser Asp Asn His 
1               5               


<210> 41
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 41
Val Pro Leu Ser Leu Tyr Ser 
1               5           


<210> 42
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 42
Val Leu Val Pro Met Ala Met Met Ala Ser 
1               5                   10  


<210> 43
<211> 4
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 43
Ala Gly Pro Arg 
1               


<210> 44
<211> 4
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 44
Ala Ala Asn Leu 
1               


<210> 45
<211> 4
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 45
Pro Thr Asn Leu 
1               


<210> 46
<211> 4
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 46
Trp Glu His Asp 
1               


<210> 47
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 47
Arg Ile Gly Phe Leu Arg 
1               5       


<210> 48
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 48
Pro Leu Gly Leu Gln Arg 
1               5       


<210> 49
<211> 8
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      probe


<220>
<221> MOD_RES
<222> (6)..(6)
<223> Lys(2,4-dinitrophenyl)

<400> 49
Lys Pro Leu Gly Leu Lys Ala Arg 
1               5               


<210> 50
<211> 8
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (4)..(4)
<223> Any small flexible amino acid

<220>
<221> MOD_RES
<222> (7)..(7)
<223> Any amino acid

<220>
<223> See specification as filed for detailed description of
      substitutions and preferred embodiments

<400> 50
Gly Arg Leu Xaa Pro Ala Xaa Leu 
1               5               


<210> 51
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (10)..(10)
<223> Lys(DNP)

<400> 51
Ile Glu Pro Asp Ser Ser Met Glu Ser Lys 
1               5                   10  


<210> 52
<211> 4
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 52
Ala Ala Pro Val 
1               


