                         SEQUENCE LISTING

<110>  VST LLC dba MEDGENE LABS
 
<120>  AFRICAN SWINE FEVER (ASF) VIRUS VACCINES

<130>  MEDG-A-ASF-PCT

<150>  US 63/128,318
<151>  2020-12-21

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  1213
<212>  DNA
<213>  African swine fever virus


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  EcoRI

<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  EcoRI Restriction Site

<220>
<221>  misc_feature
<222>  (1159)..(1175)
<223>  TEV Protease Site

<220>
<221>  misc_feature
<222>  (1176)..(1205)
<223>  HIS Tag + Stop Codon

<220>
<221>  misc_feature
<222>  (1206)..(1213)
<223>  NotI Restriction Site

<400>  1
gaattcatgg attctgaatt ttttcaaccg gtttatccgc ggcattatgg tgagtgtttg       60

tcaccagtca ctacaccaag cttcttctcc acacatatgt atactattct cattgctatc      120

gtggtcttag tcatcattat catcgttcta atctacttat tctcttcaag aaagaaaaaa      180

gctgctgcta ttgaggagga agatatacag tttataaatc cttatcaaga tcagcagtgg      240

gtagaagtca ctccacaacc aggtacctct aaaccagctg gagcgactac agcaagtgta      300

ggcaagccag tcacgggcag accggcaaca aacagaccag caacaaacaa accagttacg      360

gacaacccag ttacggacag actagtcatg gcaactggcg ggccggcagc cgctatggat      420

tttattttaa atatatccat gaaaatggag gtcatcttca aaacggattt aagatcatct      480

tcacaagttg tgtttcatgc gggtagcctg tataattggt tttctgttga gattatcaat      540

agcggtagaa ttgttacgac cgctataaaa acattgctta gtactgttaa gtatgatatt      600

gtgaaatctg ctcgtatata tgcagggcaa gggtatactg aacatcaggc tcaagaagaa      660

tggaatatga ttctgcatgt gctgtttgaa gaggagacgg aatcctcagc atcttcggag      720

aacattcatg aaaaaaatga taatgaaacc aatgaatgca catcctcctt tgaaacgttg      780

tttgagcaag agccctcatc ggaggtacct aaagactcca agctgtatat gcttgcacaa      840

aagactgtgc aacatattga acaatatgga aaggcacctg attttaacaa ggttattaga      900

gcacataatt ttattcaaac catttatgga acccctctaa aggaagaaga aaaagaggtg      960

gtaagactca tggttattaa acttttaaaa aaaataagct tttatctcac ctacattgca     1020

gccgctagtg ctcctgctca tccggctgag ccttacacga cagtcactac tcagaacact     1080

gcttcacaaa caatgtcggc tattgaaaat ttacgacaaa gaaacaccta tacgcataaa     1140

gacctagaaa actccttgaa aggcgagaac ctgtattttc aaggccacca tcatcaccat     1200

cactagcggc cgc                                                        1213


<210>  2
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  2
gtctgcgagc agttgttt                                                     18


<210>  3
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  3
cattcttctt gagcctgatg tt                                                22


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  4
catgcgggta gcctgtataa                                                   20


<210>  5
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  5
cgctctaaca taccacccta aa                                                22


<210>  6
<211>  399
<212>  PRT
<213>  African swine fever virus

<400>  6

Met Asp Ser Glu Phe Phe Gln Pro Val Tyr Pro Arg His Tyr Gly Glu 
1               5                   10                  15      


Cys Leu Ser Pro Val Thr Thr Pro Ser Phe Phe Ser Thr His Met Tyr 
            20                  25                  30          


Thr Ile Leu Ile Ala Ile Val Val Leu Val Ile Ile Ile Ile Val Leu 
        35                  40                  45              


Ile Tyr Leu Phe Ser Ser Arg Lys Lys Lys Ala Ala Ala Ile Glu Glu 
    50                  55                  60                  


Glu Asp Ile Gln Phe Ile Asn Pro Tyr Gln Asp Gln Gln Trp Val Glu 
65                  70                  75                  80  


Val Thr Pro Gln Pro Gly Thr Ser Lys Pro Ala Gly Ala Thr Thr Ala 
                85                  90                  95      


Ser Val Gly Lys Pro Val Thr Gly Arg Pro Ala Thr Asn Arg Pro Ala 
            100                 105                 110         


Thr Asn Lys Pro Val Thr Asp Asn Pro Val Thr Asp Arg Leu Val Met 
        115                 120                 125             


Ala Thr Gly Gly Pro Ala Ala Ala Met Asp Phe Ile Leu Asn Ile Ser 
    130                 135                 140                 


Met Lys Met Glu Val Ile Phe Lys Thr Asp Leu Arg Ser Ser Ser Gln 
145                 150                 155                 160 


Val Val Phe His Ala Gly Ser Leu Tyr Asn Trp Phe Ser Val Glu Ile 
                165                 170                 175     


Ile Asn Ser Gly Arg Ile Val Thr Thr Ala Ile Lys Thr Leu Leu Ser 
            180                 185                 190         


Thr Val Lys Tyr Asp Ile Val Lys Ser Ala Arg Ile Tyr Ala Gly Gln 
        195                 200                 205             


Gly Tyr Thr Glu His Gln Ala Gln Glu Glu Trp Asn Met Ile Leu His 
    210                 215                 220                 


Val Leu Phe Glu Glu Glu Thr Glu Ser Ser Ala Ser Ser Glu Asn Ile 
225                 230                 235                 240 


His Glu Lys Asn Asp Asn Glu Thr Asn Glu Cys Thr Ser Ser Phe Glu 
                245                 250                 255     


Thr Leu Phe Glu Gln Glu Pro Ser Ser Glu Val Pro Lys Asp Ser Lys 
            260                 265                 270         


Leu Tyr Met Leu Ala Gln Lys Thr Val Gln His Ile Glu Gln Tyr Gly 
        275                 280                 285             


Lys Ala Pro Asp Phe Asn Lys Val Ile Arg Ala His Asn Phe Ile Gln 
    290                 295                 300                 


Thr Ile Tyr Gly Thr Pro Leu Lys Glu Glu Glu Lys Glu Val Val Arg 
305                 310                 315                 320 


Leu Met Val Ile Lys Leu Leu Lys Lys Ile Ser Phe Tyr Leu Thr Tyr 
                325                 330                 335     


Ile Ala Ala Ala Ser Ala Pro Ala His Pro Ala Glu Pro Tyr Thr Thr 
            340                 345                 350         


Val Thr Thr Gln Asn Thr Ala Ser Gln Thr Met Ser Ala Ile Glu Asn 
        355                 360                 365             


Leu Arg Gln Arg Asn Thr Tyr Thr His Lys Asp Leu Glu Asn Ser Leu 
    370                 375                 380                 


Lys Gly Glu Asn Leu Tyr Phe Gln Gly His His His His His His 
385                 390                 395                 


<210>  7
<211>  1995
<212>  DNA
<213>  African swine fever virus

<400>  7
gaattcatgg catcaggagg agctttttgt cttattgcta acgatgggaa ggccgacaag       60

attatattgg cccaagactt gctgaatagc aggatctcta acattaaaaa tgtgaacaaa      120

agttatggga aacccgatcc cgaacccact ttgagtcaaa tcgaagaaac acatttggtg      180

cattttaatg cgcattttaa gccttatgtt ccagtagggt ttgaatacaa taaagtacgc      240

ccgcatacgg gtacccccac cttgggaaac aagcttacct ttggtattcc ccagtacgga      300

gactttttcc atgatatggt gggccatcat atattgggtg catgtcattc atcctggcag      360

gatgctccga ttcagggcac gtcccagatg ggggcccatg ggcagcttca aacgtttcct      420

cgcaacggat atgactggga caaccaaaca cccttagagg gcgccgttta cacgcttgta      480

gatccttttg gaagacccat tgtacccggc acaaagaatg cgtaccgaaa cttggtttac      540

tactgcgaat accccggaga acgactttat gaaaacgtaa gattcgatgt aaatggaaat      600

tccctagacg aatatagttc ggatgtcacc agcgttgtgc gcaaattttg catcccaggg      660

gataaaatga ctggatataa gcacttggtt ggccaggagg tatcggtgga gggaaccagt      720

ggccctctcc tatgcaacat tcatgatttg cacaagccgc accaaagcaa acctattctt      780

accgatgaaa atgatacgca gcgaacgtgt agccatacca acccgaaatt tctttcacag      840

cattttcccg agaactctca caatatccaa acagcaggta aacaagatat tactcctatc      900

acggacgcaa cgtatctgga cataagacgt aatgttcatt acagctgtaa tggacctcaa      960

acccctaaat actatcagcc ccctcttgcg ctctggatta agttgcgctt ttggtttaat     1020

gagaacgtga accttgctat tccctcagta tccattccct tcggcgagcg ctttatcacc     1080

ataaagcttg catcgcaaaa ggatttggtg aatgaatttc ctggactttt tgtacgccag     1140

tcacgtttta tagctggacg ccccagtaga cgcaatatac gctttaaacc atggtttatc     1200

ccaggagtca ttaatgaaat ctcgctcacg aataatgaac ttacatcaat aacctgtttg     1260

taacccctga aatacacaac ctttttgtaa aacgcgttcg cttttcgctg atacgtgtcc     1320

ataaaacgca ggtgacccac accaacaata accaccacga tgaaaaacta atgtctgctc     1380

ttaaatggcc cattgaatat atgtttatag gattaaaacc tacctggaac atctccgatc     1440

aaaatcctca tcaacaccga gattggcaca agttcggaca tgttgttaac gccattatgc     1500

agcccactca ccacgcagag ataagctttc aggatagaga tacagctctt ccagacgcat     1560

gttcatctat atctgatatt agccccgtta cgtatccgat cacattacct attaaaaaca     1620

tttccgtaac tgctcatggt atcaatctta tcgataaatt tccatcaaag ttctgcagct     1680

cttacatacc cttccactac ggaggcaatg cgattaaaac ccccgatgat ccgggtgcga     1740

tgatgattac ctttgctttg aagccacggg aggaatacca acccagtggt catattaacg     1800

tatccagagc aagagaattt tatattagtt gggacacgga ttacgtgggg tctatcacta     1860

cggctgatct tgtggtatcg gcatctgcta ttaactttct tcttcttcag aacggttcag     1920

ctgtgctgcg ttacagtacc aaaggcgaga acctgtattt tcaaggccac catcatcacc     1980

atcactagcg gccgc                                                      1995


<210>  8
<211>  661
<212>  PRT
<213>  African swine fever virus

<400>  8

Met Ala Ser Gly Gly Ala Phe Cys Leu Ile Ala Asn Asp Gly Lys Ala 
1               5                   10                  15      


Asp Lys Ile Ile Leu Ala Gln Asp Leu Leu Asn Ser Arg Ile Ser Asn 
            20                  25                  30          


Ile Lys Asn Val Asn Lys Ser Tyr Gly Lys Pro Asp Pro Glu Pro Thr 
        35                  40                  45              


Leu Ser Gln Ile Glu Glu Thr His Leu Val His Phe Asn Ala His Phe 
    50                  55                  60                  


Lys Pro Tyr Val Pro Val Gly Phe Glu Tyr Asn Lys Val Arg Pro His 
65                  70                  75                  80  


Thr Gly Thr Pro Thr Leu Gly Asn Lys Leu Thr Phe Gly Ile Pro Gln 
                85                  90                  95      


Tyr Gly Asp Phe Phe His Asp Met Val Gly His His Ile Leu Gly Ala 
            100                 105                 110         


Cys His Ser Ser Trp Gln Asp Ala Pro Ile Gln Gly Thr Ser Gln Met 
        115                 120                 125             


Gly Ala His Gly Gln Leu Gln Thr Phe Pro Arg Asn Gly Tyr Asp Trp 
    130                 135                 140                 


Asp Asn Gln Thr Pro Leu Glu Gly Ala Val Tyr Thr Leu Val Asp Pro 
145                 150                 155                 160 


Phe Gly Arg Pro Ile Val Pro Gly Thr Lys Asn Ala Tyr Arg Asn Leu 
                165                 170                 175     


Val Tyr Tyr Cys Glu Tyr Pro Gly Glu Arg Leu Tyr Glu Asn Val Arg 
            180                 185                 190         


Phe Asp Val Asn Gly Asn Ser Leu Asp Glu Tyr Ser Ser Asp Val Thr 
        195                 200                 205             


Thr Leu Val Arg Lys Phe Cys Ile Pro Gly Asp Lys Met Thr Gly Tyr 
    210                 215                 220                 


Lys His Leu Val Gly Gln Glu Val Ser Val Glu Gly Thr Ser Gly Pro 
225                 230                 235                 240 


Leu Leu Cys Asn Ile His Asp Leu His Lys Pro His Gln Ser Lys Pro 
                245                 250                 255     


Ile Leu Thr Asp Glu Asn Asp Thr Gln Arg Thr Cys Ser His Thr Asn 
            260                 265                 270         


Pro Lys Phe Leu Ser Gln His Phe Pro Glu Asn Ser His Asn Ile Gln 
        275                 280                 285             


Thr Ala Gly Lys Gln Asp Ile Thr Pro Ile Thr Asp Ala Tyr Thr Leu 
    290                 295                 300                 


Asp Ile Arg Arg Asn Val His Tyr Ser Cys Asn Gly Pro Gln Thr Pro 
305                 310                 315                 320 


Lys Tyr Tyr Gln Pro Pro Leu Ala Leu Trp Ile Lys Leu Arg Phe Trp 
                325                 330                 335     


Phe Asn Glu Asn Val Asn Leu Ala Ile Pro Ser Val Ser Ile Pro Phe 
            340                 345                 350         


Gly Glu Arg Phe Ile Thr Ile Leu Lys Ala Ser Gln Lys Asp Leu Val 
        355                 360                 365             


Asn Glu Phe Pro Gly Leu Phe Val Arg Gln Ser Arg Phe Ile Ala Gly 
    370                 375                 380                 


Arg Pro Ser Arg Arg Asn Ile Arg Phe Lys Pro Trp Phe Ile Pro Gly 
385                 390                 395                 400 


Val Ile Asn Glu Ile Ser Leu Thr Asn Asn Glu Leu Tyr Ile Asn Asn 
                405                 410                 415     


Leu Phe Val Thr Pro Glu Ile His Asn Leu Phe Val Lys Arg Val Arg 
            420                 425                 430         


Phe Ser Leu Ile Arg Val His Lys Thr Gln Val Thr His Thr Asn Asn 
        435                 440                 445             


Asn His His Asp Glu Lys Leu Met Ser Ala Leu Lys Trp Pro Ile Glu 
    450                 455                 460                 


Tyr Met Phe Ile Gly Leu Lys Pro Thr Trp Asn Ile Ser Asp Gln Pro 
465                 470                 475                 480 


Asn His Gln His Arg Asp Trp His Lys Phe Gly His Val Val Asn Ala 
                485                 490                 495     


Ile Met Gln Pro Thr His His Ala Glu Ile Ser Phe Gln Asp Arg Asp 
            500                 505                 510         


Thr Ala Leu Pro Asp Ala Cys Ser Ser Ile Ser Asp Ile Ser Pro Val 
        515                 520                 525             


Thr Tyr Pro Ile Thr Leu Pro Ile Ile Lys Asn Ile Ser Val Thr Ala 
    530                 535                 540                 


His Gly Ile Asn Leu Ile Asp Lys Phe Pro Ser Lys Phe Cys Ser Ser 
545                 550                 555                 560 


Tyr Ile Pro Phe His Tyr Gly Gly Asn Ala Ile Lys Thr Pro Asp Asp 
                565                 570                 575     


Pro Gly Ala Met Met Ile Thr Phe Ala Leu Lys Pro Arg Glu Glu Tyr 
            580                 585                 590         


Gln Pro Ser Gly His Ile Asn Val Ser Arg Ala Arg Glu Phe Tyr Ile 
        595                 600                 605             


Ser Trp Asp Thr Asp Tyr Val Gly Ser Ile Thr Thr Ala Asp Leu Val 
    610                 615                 620                 


Val Ser Ala Ser Ala Ile Asn Phe Leu Leu Leu Gln Asn Gly Ser Ala 
625                 630                 635                 640 


Val Leu Arg Tyr Ser Thr Lys Gly Glu Asn Leu Tyr Phe Gln Gly His 
                645                 650                 655     


His His His His His 
            660     


<210>  9
<211>  664
<212>  DNA
<213>  African swine fever virus

<400>  9
gaattcatgg attttatttt aaatatatcc atgaaaatgg aggtcatctt caaaacggat       60

ttaagatcat cttcacaagt tgtgtttcat gcgggtagcc tgtataattg gttttctgtt      120

gagattatca atagcggtag aattgttacg accgctataa aaacattgct tagtactgtt      180

aagtatgata ttgtgaaatc tgctcgtata tatgcagggc aagggtatac tgaacatcag      240

gctcaagaag aatggaatat gattctgcat gtgctgtttg aagaggagac ggaatcctca      300

gcatcttcgg agaacattca tgaaaaaaat gataatgaaa ccaatgaatg cacatcctcc      360

tttgaaacgt tgtttgagca agagccctca tcggaggtac ctaaagactc caagctgtat      420

atgcttgcac aaaagactgt gcaacatatt gaacaatatg gaaaggcacc tgattttaac      480

aaggttatta gagcacataa ttttattcaa accatttatg gaacccctct aaaggaagaa      540

gaaaaagagg tggtaagact catggttatt aaacttttaa aaaaaataag cttttatctc      600

acctacatta aaggcgagaa cctgtatttt caaggccacc atcatcacca tcactagcgg      660

ccgc                                                                   664


<210>  10
<211>  216
<212>  PRT
<213>  African swine fever virus

<400>  10

Met Asp Phe Ile Leu Asn Ile Ser Met Lys Met Glu Val Ile Phe Lys 
1               5                   10                  15      


Thr Asp Leu Arg Ser Ser Ser Gln Val Val Phe His Ala Gly Ser Leu 
            20                  25                  30          


Tyr Asn Trp Phe Ser Val Glu Ile Ile Asn Ser Gly Arg Ile Val Thr 
        35                  40                  45              


Thr Ala Ile Lys Thr Leu Leu Ser Thr Val Lys Tyr Asp Ile Val Lys 
    50                  55                  60                  


Ser Ala Arg Ile Tyr Ala Gly Gln Gly Tyr Thr Glu His Gln Ala Gln 
65                  70                  75                  80  


Glu Glu Trp Asn Met Ile Leu His Val Leu Phe Glu Glu Glu Thr Glu 
                85                  90                  95      


Ser Ser Ala Ser Ser Glu Asn Ile His Glu Lys Asn Asp Asn Glu Thr 
            100                 105                 110         


Asn Glu Cys Thr Ser Ser Phe Glu Thr Leu Phe Glu Gln Glu Pro Ser 
        115                 120                 125             


Ser Glu Val Pro Lys Asp Ser Lys Leu Tyr Met Leu Ala Gln Lys Thr 
    130                 135                 140                 


Val Gln His Ile Glu Gln Tyr Gly Lys Ala Pro Asp Phe Asn Lys Val 
145                 150                 155                 160 


Ile Arg Ala His Asn Phe Ile Gln Thr Ile Tyr Gly Thr Pro Leu Lys 
                165                 170                 175     


Glu Glu Glu Lys Glu Val Val Arg Leu Met Val Ile Lys Leu Leu Lys 
            180                 185                 190         


Lys Ile Ser Phe Tyr Leu Thr Tyr Ile Lys Gly Glu Asn Leu Tyr Phe 
        195                 200                 205             


Gln Gly His His His His His His 
    210                 215     


<210>  11
<211>  613
<212>  DNA
<213>  African swine fever virus

<400>  11
gaattcatgg attctgaatt ttttcaaccg gtttatccgc ggcattatgg tgagtgtttg       60

tcaccagtca ctacaccaag cttcttctcc acacatatgt atactattct cattgctatc      120

gtggtcttag tcatcattat catcgttcta atctatctat tctcttcaag aaagaaaaaa      180

gctgctgcta ttgaggagga agatatacag tttataaatc cttatcaaga tcagcagtgg      240

gtagaagtca ctccacaacc aggtacctct aaaccagctg gagcgactac agcaagtgta      300

ggcaagccag tcacgggcag accggcaaca aacagaccag caacaaacaa accagttacg      360

gacaacccag ttacggacag actagtcatg gcaactggcg ggccggctgc tgcacctgca      420

gctgcgagtg ctcctgctca tccggctgag ccttacacga cagtcactac tcagaacact      480

gcttcacaaa caatgtcggc tattgaaaat ttacgacaaa gaaacaccta tacgcataaa      540

gacctagaaa actccttgaa aggcgagaac ctgtattttc aaggccacca tcatcaccat      600

cactagcggc cgc                                                         613


<210>  12
<211>  199
<212>  PRT
<213>  African swine fever virus

<400>  12

Met Asp Ser Glu Phe Phe Gln Pro Val Tyr Pro Arg His Tyr Gly Glu 
1               5                   10                  15      


Cys Leu Ser Pro Val Thr Thr Pro Ser Phe Phe Ser Thr His Met Tyr 
            20                  25                  30          


Thr Ile Leu Ile Ala Ile Val Val Leu Val Ile Ile Ile Ile Val Leu 
        35                  40                  45              


Ile Tyr Leu Phe Ser Ser Arg Lys Lys Lys Ala Ala Ala Ile Glu Glu 
    50                  55                  60                  


Glu Asp Ile Gln Phe Ile Asn Pro Tyr Gln Asp Gln Gln Trp Val Glu 
65                  70                  75                  80  


Val Thr Pro Gln Pro Gly Thr Ser Lys Pro Ala Gly Ala Thr Thr Ala 
                85                  90                  95      


Ser Val Gly Lys Pro Val Thr Gly Arg Pro Ala Thr Asn Arg Pro Ala 
            100                 105                 110         


Thr Asn Lys Pro Val Thr Asp Asn Pro Val Thr Asp Arg Leu Val Met 
        115                 120                 125             


Ala Thr Gly Gly Pro Ala Ala Ala Pro Ala Ala Ala Ser Ala Pro Ala 
    130                 135                 140                 


His Pro Ala Glu Pro Tyr Thr Thr Val Thr Thr Gln Asn Thr Ala Ser 
145                 150                 155                 160 


Gln Thr Met Ser Ala Ile Glu Asn Leu Arg Gln Arg Asn Thr Tyr Thr 
                165                 170                 175     


His Lys Asp Leu Glu Asn Ser Leu Lys Gly Glu Asn Leu Tyr Phe Gln 
            180                 185                 190         


Gly His His His His His His 
        195                 


<210>  13
<211>  1083
<212>  DNA
<213>  African swine fever virus

<400>  13
atgataatac ttattttttt aatattttct aacatagttt taagtattga ttattgggtt       60

agttttaata aaacaataat tttagatagt aatattacta atgataataa tgatataaat      120

ggagtatcat ggaatttttt taataattct tttaatacac tagctacatg tggaaaagca      180

ggtaactttt gtgaatgttc taattatagt acatcaatat ataatataac aaataattgt      240

agcttaacta tttttcctca taatgatgta tttgatacaa catatcaagt agtatggaat      300

caaataatta attatacaat aaaattatta acacctgcta ctcccccaaa tatcacatat      360

aattgtacta attttttaat aacatgtaaa aaaaataatg gaacaaacac taatatatat      420

ttaaatataa atgatacttt tgttaaatat actaatgaaa gtatacttga atataactgg      480

aataatagta acattaacaa ttttacagct acatgtataa ttaataatac aattagtaca      540

tctaatgaaa caacacttat aaattgtact tatttaacat tgtcatctaa ctatttttat      600

acttttttta aattatatta tattccatta agcatcataa ttgggataac aataagtatt      660

cttcttatat ccatcataac ttttttatct ttacgaaaaa gaaaaaaaca tgttgaagaa      720

atagaaagtc caccacctga atctaatgaa gaagaacaat gtcagcatga tgacaccact      780

tccatacatg aaccatctcc cagagaacca ttacttccta agccttacag tcgttatcag      840

tataatacac ctatttacta catgcgtccc tcaacacaac cactcaaccc atttccctta      900

cctaaaccgt gtcctccacc caaaccatgt ccgccaccca aaccatgtcc tccacctaaa      960

ccatgtcctt cagctgaatc ctattctcca cccaaaccac tacctagtat cccgctacta     1020

cccaatatcc cgccattatc tacccaaaat atttcgctta ttcacgtaga tagaattatt     1080

taa                                                                   1083


<210>  14
<211>  1160
<212>  DNA
<213>  African swine fever virus


<220>
<221>  misc_feature
<222>  (10)..(15)
<223>  EcoRI Restriction Site

<220>
<221>  misc_feature
<222>  (1096)..(1122)
<223>  TEV Restriction Site

<220>
<221>  misc_feature
<222>  (1123)..(1143)
<223>  HIS Tag + Stop Codon

<220>
<221>  misc_feature
<222>  (1151)..(1156)
<223>  NotI Restriction Site

<400>  14
ggacctaagg aattcatgat aattctgatc tttttgatat tcagtaatat cgtgttatcg       60

atagactact gggtgtcttt caataagact atcattttag actcaaacat taccaatgac      120

aacaacgata tcaatggagt ctcttggaat ttttttaaca acagtttcaa cactcttgca      180

acttgcggga aggctggtaa tttttgtgag tgtagcaact actccacgtc tatctataat      240

attacaaata actgttcatt gactattttt cctcataacg acgtattcga cacaacatac      300

caggtagtgt ggaatcaaat aataaattac actatcaaac ttctgacacc ggcgacgccc      360

cccaacatca catataattg tacgaatttc cttataacat gcaaaaagaa caacggtacc      420

aatactaata tctacctgaa catcaacgat accttcgtta aatatactaa tgagtcgatc      480

ttagagtaca actggaacaa ttctaatatt aacaatttta ctgccacttg tataataaat      540

aacactataa gtacgtccaa tgagaccacg cttatcaact gcacatattt gacactatct      600

tctaactatt tttatacgtt ttttaagttg tattatatcc ctctgtcaat catcataggg      660

ataacaatca gcatacttct catttcaatt atcacttttt taagccttcg taaacgcaag      720

aagcatgtgg aagaaataga atctcctccg ccggagagca atgaagagga acaatgtcag      780

catgacgata caacatcaat ccatgagcca tcgcctagag agccactgct gcccaaacct      840

tattcacgtt atcaatacaa tacgccaata tattacatgc gccctagcac acagccacta      900

aatccgtttc cgctgccgaa gccgtgtcca ccccctaagc cgtgcccacc gcctaaaccc      960

tgccctcctc ccaagccatg tccctcggca gagtcatatt ccccacctaa gcccttaccg     1020

tccatccctc tgctaccaaa tatacctccc ctgagtaccc aaaatatttc cttaatccat     1080

gtagaccgaa tcatcaaggg cgaaaacttg tactttcaag gccatcacca tcaccatcac     1140

taggcggccg caattgccaa                                                 1160


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  15
cggcggagga gattctattt                                                   20


<210>  16
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  16
tccaatgaga ccacgcttat c                                                 21


<210>  17
<211>  375
<212>  PRT
<213>  African swine fever virus

<400>  17

Met Ile Ile Leu Ile Phe Leu Ile Phe Ser Asn Ile Val Leu Ser Ile 
1               5                   10                  15      


Asp Tyr Trp Val Ser Phe Asn Lys Thr Ile Ile Leu Asp Ser Asn Ile 
            20                  25                  30          


Thr Asn Asp Asn Asn Asp Ile Asn Gly Val Ser Trp Asn Phe Phe Asn 
        35                  40                  45              


Asn Ser Phe Asn Thr Leu Ala Thr Cys Gly Lys Ala Gly Asn Phe Cys 
    50                  55                  60                  


Glu Cys Ser Asn Tyr Ser Thr Ser Ile Tyr Asn Ile Thr Asn Asn Cys 
65                  70                  75                  80  


Ser Leu Thr Ile Phe Pro His Asn Asp Val Phe Asp Thr Thr Tyr Gln 
                85                  90                  95      


Val Val Trp Asn Gln Ile Ile Asn Tyr Thr Ile Lys Leu Leu Thr Pro 
            100                 105                 110         


Ala Thr Pro Pro Asn Ile Thr Tyr Asn Cys Thr Asn Phe Leu Ile Thr 
        115                 120                 125             


Cys Lys Lys Asn Asn Gly Thr Asn Thr Asn Ile Tyr Leu Asn Ile Asn 
    130                 135                 140                 


Asp Thr Phe Val Lys Tyr Thr Asn Glu Ser Ile Leu Glu Tyr Asn Trp 
145                 150                 155                 160 


Asn Asn Ser Asn Ile Asn Asn Phe Thr Ala Thr Cys Ile Ile Asn Asn 
                165                 170                 175     


Thr Ile Ser Thr Ser Asn Glu Thr Thr Leu Ile Asn Cys Thr Tyr Leu 
            180                 185                 190         


Thr Leu Ser Ser Asn Tyr Phe Tyr Thr Phe Phe Lys Leu Tyr Tyr Ile 
        195                 200                 205             


Pro Leu Ser Ile Ile Ile Gly Ile Thr Ile Ser Ile Leu Leu Ile Ser 
    210                 215                 220                 


Ile Ile Thr Phe Leu Ser Leu Arg Lys Arg Lys Lys His Val Glu Glu 
225                 230                 235                 240 


Ile Glu Ser Pro Pro Pro Glu Ser Asn Glu Glu Glu Gln Cys Gln His 
                245                 250                 255     


Asp Asp Thr Thr Ser Ile His Glu Pro Ser Pro Arg Glu Pro Leu Leu 
            260                 265                 270         


Pro Lys Pro Tyr Ser Arg Tyr Gln Tyr Asn Thr Pro Ile Tyr Tyr Met 
        275                 280                 285             


Arg Pro Ser Thr Gln Pro Leu Asn Pro Phe Pro Leu Pro Lys Pro Cys 
    290                 295                 300                 


Pro Pro Pro Lys Pro Cys Pro Pro Pro Lys Pro Cys Pro Pro Pro Lys 
305                 310                 315                 320 


Pro Cys Pro Ser Ala Glu Ser Tyr Ser Pro Pro Lys Pro Leu Pro Ser 
                325                 330                 335     


Ile Pro Leu Leu Pro Asn Ile Pro Pro Leu Ser Thr Gln Asn Ile Ser 
            340                 345                 350         


Leu Ile His Val Asp Arg Ile Ile Lys Gly Glu Asn Leu Tyr Phe Gln 
        355                 360                 365             


Gly His His His His His His 
    370                 375 


