                         SEQUENCE LISTING

<110>  2seventy bio, Inc.
       Jarjour, Jordan
       Krostag, Anne-Rachel
 
<120>  COMPOSITIONS AND METHODS FOR SITE-DIRECTED MUTAGENESIS

<130>  BLUE-132.PC

<150>  US 63/128,391
<151>  2020-12-21

<160>  164   

<170>  PatentIn version 3.5

<210>  1
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  1
tgtctgccta ttcaccgatt tt                                                22


<210>  2
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  2
ggcatgcaga tcccacaggc gc                                                22


<210>  3
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  3
ctgtaagata ttcccatccc ca                                                22


<210>  4
<211>  14
<212>  DNA
<213>  Homo sapiens

<400>  4
tggtggggct gctc                                                         14


<210>  5
<211>  2637
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL DNA construct

<400>  5
atgggatcct gcaggccacc taagaagaaa cgcaaagtcg tggatctacg cacgctcggc       60

tacagtcagc agcagcaaga gaagatcaaa ccgaaggtgc gttcgacagt ggcgcagcac      120

cacgaggcac tggtgggcca tgggtttaca cacgcgcaca tcgttgcgct cagccaacac      180

ccggcagcgt tagggaccgt cgctgtcacg tatcagcaca taatcacggc gttgccagag      240

gcgacacacg aagacatcgt tggcgtcggc aaacagtggt ccggcgcacg cgccctggag      300

gccttgctca cggatgcggg ggagttgaga ggtccgccgt tacagttgga cacaggccaa      360

cttgtgaaga ttgcaaaacg tggcggcgtg accgcaatgg aggcagtgca tgcatcgcgc      420

aatgcactga cgggtgcccc cctgaacctg accccggacc aagtggtggc tatcgccagc      480

aacattggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag      540

gaccatggcc tgaccccgga ccaagtggtg gctatcgcca gcaacattgg cggcaagcaa      600

gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg      660

gaccaagtgg tggctatcgc cagcaacatt ggcggcaagc aagcgctcga aacggtgcag      720

cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc      780

gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg      840

tgccaggacc atggcctgac tccggaccaa gtggtggcta tcgccagcca cgatggcggc      900

aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg      960

actccggacc aagtggtggc tatcgccagc cacgatggcg gcaagcaagc gctcgaaacg     1020

gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg     1080

gctatcgcca gcaacattgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg     1140

gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc cagcaacaat     1200

ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat     1260

ggcctgaccc cggaccaagt ggtggctatc gccagcaacg gtggcggcaa gcaagcgctc     1320

gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa     1380

gtggtggcta tcgccagcaa caatggcggc aagcaagcgc tcgaaacggt gcagcggctg     1440

ttgccggtgc tgtgccagga ccatggcctg accccggacc aagtggtggc tatcgccagc     1500

aacattggcg gcaagcaagc gctcgaaagc attgtggccc agctgagccg gcctgatccg     1560

gcgttggccg cgttgaccaa cgaccacctc gtcgccttgg cctgcctcgg cggacgtcct     1620

gccatggatg cagtgaaaaa gggattgccg cacgcgccgg aattgatcag aagagtcaat     1680

cgccgtattg gcgaacgcac gtcccatcgc gttgcgatat ctagagtggg aggaagctct     1740

cgcagagagt ccatcaaccc atggattctg actggtttcg ctgatgccga aggatcattc     1800

atactagaca tccgcaaccg aaacaacgaa agcaacagat accgaacttc gctgagattc     1860

cagatcaccc tgcacaacaa ggacaaatcg attctggaga atatccagtc cacttggaag     1920

gtcggcaaga tcacaaacag cagtgacaga gccgtcatgc tgagggtcac ccgtttcgaa     1980

gatttgaaag tgattatcga ccacttcgag aaatatccgc tgattaccca gaaattgggc     2040

gattacaagt tgtttaaaca ggcattcagc gtcatggaga ataaagaaca tcttaaggag     2100

aatgggatta aggagctcgt acgaatcaaa gctaagatga attggggtct caatgacgaa     2160

ttgaaaaaag catttccaga gaacattagc aaagagcgcc cccttatcaa taagaacatt     2220

ccgaatttca aatggctggc tggattcaca gctggtgaag gctacttcgg cgtgaatcta     2280

aaaaaggtaa agggcaccgc aaaggtatac gtgggactga gattctcaat ctcacagcac     2340

atcagagaca agaacctgat gaattcattg ataacatacc taggctgtgg ttccatctgg     2400

gagaagaaca agtctgagtt cagatggctc gagttcgtcg taaccaaatt cagcgatatc     2460

aacgacaaga tcattccggt attccaggaa aatactctga ttggcgtcaa actcgaggac     2520

tttgaagatt ggtgcaaggt tgccaaattg atcaaagaga agaaacacct gaccgaatcc     2580

ggtttggatg agattaagaa aatcaagctg aacatgaaca aaggtcgtgt cttctga        2637


<210>  6
<211>  2637
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL RNA construct

<400>  6
augggauccu gcaggccacc uaagaagaaa cgcaaagucg uggaucuacg cacgcucggc       60

uacagucagc agcagcaaga gaagaucaaa ccgaaggugc guucgacagu ggcgcagcac      120

cacgaggcac uggugggcca uggguuuaca cacgcgcaca ucguugcgcu cagccaacac      180

ccggcagcgu uagggaccgu cgcugucacg uaucagcaca uaaucacggc guugccagag      240

gcgacacacg aagacaucgu uggcgucggc aaacaguggu ccggcgcacg cgcccuggag      300

gccuugcuca cggaugcggg ggaguugaga gguccgccgu uacaguugga cacaggccaa      360

cuugugaaga uugcaaaacg uggcggcgug accgcaaugg aggcagugca ugcaucgcgc      420

aaugcacuga cgggugcccc ccugaaccug accccggacc aagugguggc uaucgccagc      480

aacauuggcg gcaagcaagc gcucgaaacg gugcagcggc uguugccggu gcugugccag      540

gaccauggcc ugaccccgga ccaaguggug gcuaucgcca gcaacauugg cggcaagcaa      600

gcgcucgaaa cggugcagcg gcuguugccg gugcugugcc aggaccaugg ccugaccccg      660

gaccaagugg uggcuaucgc cagcaacauu ggcggcaagc aagcgcucga aacggugcag      720

cggcuguugc cggugcugug ccaggaccau ggccugaccc cggaccaagu gguggcuauc      780

gccagcaacg guggcggcaa gcaagcgcuc gaaacggugc agcggcuguu gccggugcug      840

ugccaggacc auggccugac uccggaccaa gugguggcua ucgccagcca cgauggcggc      900

aagcaagcgc ucgaaacggu gcagcggcug uugccggugc ugugccagga ccauggccug      960

acuccggacc aagugguggc uaucgccagc cacgauggcg gcaagcaagc gcucgaaacg     1020

gugcagcggc uguugccggu gcugugccag gaccauggcc ugaccccgga ccaaguggug     1080

gcuaucgcca gcaacauugg cggcaagcaa gcgcucgaaa cggugcagcg gcuguugccg     1140

gugcugugcc aggaccaugg ccugaccccg gaccaagugg uggcuaucgc cagcaacaau     1200

ggcggcaagc aagcgcucga aacggugcag cggcuguugc cggugcugug ccaggaccau     1260

ggccugaccc cggaccaagu gguggcuauc gccagcaacg guggcggcaa gcaagcgcuc     1320

gaaacggugc agcggcuguu gccggugcug ugccaggacc auggccugac cccggaccaa     1380

gugguggcua ucgccagcaa caauggcggc aagcaagcgc ucgaaacggu gcagcggcug     1440

uugccggugc ugugccagga ccauggccug accccggacc aagugguggc uaucgccagc     1500

aacauuggcg gcaagcaagc gcucgaaagc auuguggccc agcugagccg gccugauccg     1560

gcguuggccg cguugaccaa cgaccaccuc gucgccuugg ccugccucgg cggacguccu     1620

gccauggaug cagugaaaaa gggauugccg cacgcgccgg aauugaucag aagagucaau     1680

cgccguauug gcgaacgcac gucccaucgc guugcgauau cuagaguggg aggaagcucu     1740

cgcagagagu ccaucaaccc auggauucug acugguuucg cugaugccga aggaucauuc     1800

auacuagaca uccgcaaccg aaacaacgaa agcaacagau accgaacuuc gcugagauuc     1860

cagaucaccc ugcacaacaa ggacaaaucg auucuggaga auauccaguc cacuuggaag     1920

gucggcaaga ucacaaacag cagugacaga gccgucaugc ugagggucac ccguuucgaa     1980

gauuugaaag ugauuaucga ccacuucgag aaauauccgc ugauuaccca gaaauugggc     2040

gauuacaagu uguuuaaaca ggcauucagc gucauggaga auaaagaaca ucuuaaggag     2100

aaugggauua aggagcucgu acgaaucaaa gcuaagauga auuggggucu caaugacgaa     2160

uugaaaaaag cauuuccaga gaacauuagc aaagagcgcc cccuuaucaa uaagaacauu     2220

ccgaauuuca aauggcuggc uggauucaca gcuggugaag gcuacuucgg cgugaaucua     2280

aaaaagguaa agggcaccgc aaagguauac gugggacuga gauucucaau cucacagcac     2340

aucagagaca agaaccugau gaauucauug auaacauacc uaggcugugg uuccaucugg     2400

gagaagaaca agucugaguu cagauggcuc gaguucgucg uaaccaaauu cagcgauauc     2460

aacgacaaga ucauuccggu auuccaggaa aauacucuga uuggcgucaa acucgaggac     2520

uuugaagauu ggugcaaggu ugccaaauug aucaaagaga agaaacaccu gaccgaaucc     2580

gguuuggaug agauuaagaa aaucaagcug aacaugaaca aaggucgugu cuucuga        2637


<210>  7
<211>  878
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL protein construct

<400>  7

Met Gly Ser Cys Arg Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser Ile Val 
            500                 505                 510         


Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp 
        515                 520                 525             


His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala 
    530                 535                 540                 


Val Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn 
545                 550                 555                 560 


Arg Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val 
                565                 570                 575     


Gly Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly 
            580                 585                 590         


Phe Ala Asp Ala Glu Gly Ser Phe Ile Leu Asp Ile Arg Asn Arg Asn 
        595                 600                 605             


Asn Glu Ser Asn Arg Tyr Arg Thr Ser Leu Arg Phe Gln Ile Thr Leu 
    610                 615                 620                 


His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys 
625                 630                 635                 640 


Val Gly Lys Ile Thr Asn Ser Ser Asp Arg Ala Val Met Leu Arg Val 
                645                 650                 655     


Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr 
            660                 665                 670         


Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala 
        675                 680                 685             


Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys 
    690                 695                 700                 


Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp Glu 
705                 710                 715                 720 


Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile 
                725                 730                 735     


Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ala Gly 
            740                 745                 750         


Glu Gly Tyr Phe Gly Val Asn Leu Lys Lys Val Lys Gly Thr Ala Lys 
        755                 760                 765             


Val Tyr Val Gly Leu Arg Phe Ser Ile Ser Gln His Ile Arg Asp Lys 
    770                 775                 780                 


Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Ser Ile Trp 
785                 790                 795                 800 


Glu Lys Asn Lys Ser Glu Phe Arg Trp Leu Glu Phe Val Val Thr Lys 
                805                 810                 815     


Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr 
            820                 825                 830         


Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala 
        835                 840                 845             


Lys Leu Ile Lys Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu 
    850                 855                 860                 


Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe 
865                 870                 875             


<210>  8
<211>  2730
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL DNA construct

<400>  8
atgggatccg cgccacctaa gaagaaacgc aaagtcgtgg atctacgcac gctcggctac       60

agtcagcagc agcaagagaa gatcaaaccg aaggtgcgtt cgacagtggc gcagcaccac      120

gaggcactgg tgggccatgg gtttacacac gcgcacatcg ttgcgctcag ccaacacccg      180

gcagcgttag ggaccgtcgc tgtcacgtat cagcacataa tcacggcgtt gccagaggcg      240

acacacgaag acatcgttgg cgtcggcaaa cagtggtccg gcgcacgcgc cctggaggcc      300

ttgctcacgg atgcggggga gttgagaggt ccgccgttac agttggacac aggccaactt      360

gtgaagattg caaaacgtgg cggcgtgacc gcaatggagg cagtgcatgc atcgcgcaat      420

gcactgacgg gtgcccccct gaacctgacc ccggaccaag tggtggctat cgccagcaac      480

attggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac      540

catggcctga ccccggacca agtggtggct atcgccagca acattggcgg caagcaagcg      600

ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac      660

caagtggtgg ctatcgccag caacattggc ggcaagcaag cgctcgaaac ggtgcagcgg      720

ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc      780

agcaacggtg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc      840

caggaccatg gcctgactcc ggaccaagtg gtggctatcg ccagccacga tggcggcaag      900

caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgact      960

ccggaccaag tggtggctat cgccagccac gatggcggca agcaagcgct cgaaacggtg     1020

cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct     1080

atcgccagca acattggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg     1140

ctgtgccagg accatggcct gaccccggac caagtggtgg ctatcgccag caacaatggc     1200

ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc     1260

ctgaccccgg accaagtggt ggctatcgcc agcaacggtg gcggcaagca agcgctcgaa     1320

acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg     1380

gtggctatcg ccagcaacaa tggcggcaag caagcgctcg aaacggtgca gcggctgttg     1440

ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagcaac     1500

attggcggca agcaagcgct cgaaaccgta caacgtctcc tcccagtact ttgtcaagac     1560

cacgggttga ctccggatca agtcgtcgcg atcgcgagcc atgatggggg gaagcaggcg     1620

ctggaaagca ttgtggccca gctgagccgg cctgatccgg cgttggccgc gttgaccaac     1680

gaccacctcg tcgccttggc ctgcctcggc ggacgtcctg ccatggatgc agtgaaaaag     1740

ggattgccgc acgcgccgga attgatcaga agagtcaatc gccgtattgg cgaacgcacg     1800

tcccatcgcg ttgcgatatc tagagtggga ggaagctcgc gcagagagtc catcaaccca     1860

tggattctga ctggtttcgc tgatgccgaa ggatcattca tactagacat ccgcaaccga     1920

aacaacgaaa gcaacagata ccgaacttcg ctgagattcc agatcaccct gcacaacaag     1980

gacaaatcga ttctggagaa tatccagtcc acttggaagg tcggcaagat cacaaacagc     2040

agtgacagag ccgtcatgct gagggtcacc cgtttcgaag atttgaaagt gattatcgac     2100

cacttcgaga aatatccgct gattacccag aaattgggcg attacaagtt gtttaaacag     2160

gcattcagcg tcatggagaa taaagaacat cttaaggaga atgggattaa ggagctcgta     2220

cgaatcaaag ctaagatgaa ttggggtctc aatgacgaat tgaaaaaagc atttccagag     2280

aacattagca aagagcgccc ccttatcaat aagaacattc cgaatttcaa atggctggct     2340

ggattcacag ctggtgatgg ccatttcggc gtgaatctaa aaaaggtaaa gggcaccgca     2400

aaggtatacg tgggactgag attcgctatc tcacagcaca tcagagacaa gaacctgatg     2460

aattcattga taacatacct aggctgtggt tccatcagag agaagaacaa gtctgagttc     2520

agatggctcg agttcgaagt aaccaaattc agcgatatca acgacaagat cattccggta     2580

ttccaggaaa atactctgat tggcgtcaaa ctcgaggact ttgaagattg gtgcaaggtt     2640

gccaaattga tcgaagagaa gaaacacctg accgaatccg gtttggatga gattaagaaa     2700

atcaagctga acatgaacaa aggtcgttga                                      2730


<210>  9
<211>  2730
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL RNA construct

<400>  9
augggauccg cgccaccuaa gaagaaacgc aaagucgugg aucuacgcac gcucggcuac       60

agucagcagc agcaagagaa gaucaaaccg aaggugcguu cgacaguggc gcagcaccac      120

gaggcacugg ugggccaugg guuuacacac gcgcacaucg uugcgcucag ccaacacccg      180

gcagcguuag ggaccgucgc ugucacguau cagcacauaa ucacggcguu gccagaggcg      240

acacacgaag acaucguugg cgucggcaaa cagugguccg gcgcacgcgc ccuggaggcc      300

uugcucacgg augcggggga guugagaggu ccgccguuac aguuggacac aggccaacuu      360

gugaagauug caaaacgugg cggcgugacc gcaauggagg cagugcaugc aucgcgcaau      420

gcacugacgg gugccccccu gaaccugacc ccggaccaag ugguggcuau cgccagcaac      480

auuggcggca agcaagcgcu cgaaacggug cagcggcugu ugccggugcu gugccaggac      540

cauggccuga ccccggacca agugguggcu aucgccagca acauuggcgg caagcaagcg      600

cucgaaacgg ugcagcggcu guugccggug cugugccagg accauggccu gaccccggac      660

caaguggugg cuaucgccag caacauuggc ggcaagcaag cgcucgaaac ggugcagcgg      720

cuguugccgg ugcugugcca ggaccauggc cugaccccgg accaaguggu ggcuaucgcc      780

agcaacggug gcggcaagca agcgcucgaa acggugcagc ggcuguugcc ggugcugugc      840

caggaccaug gccugacucc ggaccaagug guggcuaucg ccagccacga uggcggcaag      900

caagcgcucg aaacggugca gcggcuguug ccggugcugu gccaggacca uggccugacu      960

ccggaccaag ugguggcuau cgccagccac gauggcggca agcaagcgcu cgaaacggug     1020

cagcggcugu ugccggugcu gugccaggac cauggccuga ccccggacca agugguggcu     1080

aucgccagca acauuggcgg caagcaagcg cucgaaacgg ugcagcggcu guugccggug     1140

cugugccagg accauggccu gaccccggac caaguggugg cuaucgccag caacaauggc     1200

ggcaagcaag cgcucgaaac ggugcagcgg cuguugccgg ugcugugcca ggaccauggc     1260

cugaccccgg accaaguggu ggcuaucgcc agcaacggug gcggcaagca agcgcucgaa     1320

acggugcagc ggcuguugcc ggugcugugc caggaccaug gccugacccc ggaccaagug     1380

guggcuaucg ccagcaacaa uggcggcaag caagcgcucg aaacggugca gcggcuguug     1440

ccggugcugu gccaggacca uggccugacc ccggaccaag ugguggcuau cgccagcaac     1500

auuggcggca agcaagcgcu cgaaaccgua caacgucucc ucccaguacu uugucaagac     1560

cacggguuga cuccggauca agucgucgcg aucgcgagcc augauggggg gaagcaggcg     1620

cuggaaagca uuguggccca gcugagccgg ccugauccgg cguuggccgc guugaccaac     1680

gaccaccucg ucgccuuggc cugccucggc ggacguccug ccauggaugc agugaaaaag     1740

ggauugccgc acgcgccgga auugaucaga agagucaauc gccguauugg cgaacgcacg     1800

ucccaucgcg uugcgauauc uagaguggga ggaagcucgc gcagagaguc caucaaccca     1860

uggauucuga cugguuucgc ugaugccgaa ggaucauuca uacuagacau ccgcaaccga     1920

aacaacgaaa gcaacagaua ccgaacuucg cugagauucc agaucacccu gcacaacaag     1980

gacaaaucga uucuggagaa uauccagucc acuuggaagg ucggcaagau cacaaacagc     2040

agugacagag ccgucaugcu gagggucacc cguuucgaag auuugaaagu gauuaucgac     2100

cacuucgaga aauauccgcu gauuacccag aaauugggcg auuacaaguu guuuaaacag     2160

gcauucagcg ucauggagaa uaaagaacau cuuaaggaga augggauuaa ggagcucgua     2220

cgaaucaaag cuaagaugaa uuggggucuc aaugacgaau ugaaaaaagc auuuccagag     2280

aacauuagca aagagcgccc ccuuaucaau aagaacauuc cgaauuucaa auggcuggcu     2340

ggauucacag cuggugaugg ccauuucggc gugaaucuaa aaaagguaaa gggcaccgca     2400

aagguauacg ugggacugag auucgcuauc ucacagcaca ucagagacaa gaaccugaug     2460

aauucauuga uaacauaccu aggcuguggu uccaucagag agaagaacaa gucugaguuc     2520

agauggcucg aguucgaagu aaccaaauuc agcgauauca acgacaagau cauuccggua     2580

uuccaggaaa auacucugau uggcgucaaa cucgaggacu uugaagauug gugcaagguu     2640

gccaaauuga ucgaagagaa gaaacaccug accgaauccg guuuggauga gauuaagaaa     2700

aucaagcuga acaugaacaa aggucguuga                                      2730


<210>  10
<211>  909
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL protein construct

<400>  10

Met Gly Ser Ala Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu Arg 
1               5                   10                  15      


Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys Val 
            20                  25                  30          


Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly Phe 
        35                  40                  45              


Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu Gly 
    50                  55                  60                  


Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu Ala 
65                  70                  75                  80  


Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala Arg 
                85                  90                  95      


Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro Pro 
            100                 105                 110         


Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly Gly 
        115                 120                 125             


Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr Gly 
    130                 135                 140                 


Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
145                 150                 155                 160 


Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
                165                 170                 175     


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
            180                 185                 190         


Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
        195                 200                 205             


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
    210                 215                 220                 


Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
225                 230                 235                 240 


Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val 
                245                 250                 255     


Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val 
            260                 265                 270         


Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp 
        275                 280                 285             


Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu 
    290                 295                 300                 


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr 
305                 310                 315                 320 


Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala 
                325                 330                 335     


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly 
            340                 345                 350         


Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys 
        355                 360                 365             


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp 
    370                 375                 380                 


His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly 
385                 390                 395                 400 


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
                405                 410                 415     


Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
            420                 425                 430         


Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
        435                 440                 445             


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
    450                 455                 460                 


Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
465                 470                 475                 480 


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
                485                 490                 495     


Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
            500                 505                 510         


Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val 
        515                 520                 525             


Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Ser Ile 
    530                 535                 540                 


Val Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn 
545                 550                 555                 560 


Asp His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp 
                565                 570                 575     


Ala Val Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val 
            580                 585                 590         


Asn Arg Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg 
        595                 600                 605             


Val Gly Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
    610                 615                 620                 


Gly Phe Ala Asp Ala Glu Gly Ser Phe Ile Leu Asp Ile Arg Asn Arg 
625                 630                 635                 640 


Asn Asn Glu Ser Asn Arg Tyr Arg Thr Ser Leu Arg Phe Gln Ile Thr 
                645                 650                 655     


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
            660                 665                 670         


Lys Val Gly Lys Ile Thr Asn Ser Ser Asp Arg Ala Val Met Leu Arg 
        675                 680                 685             


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
    690                 695                 700                 


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
705                 710                 715                 720 


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
                725                 730                 735     


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
            740                 745                 750         


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
        755                 760                 765             


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ala 
    770                 775                 780                 


Gly Asp Gly His Phe Gly Val Asn Leu Lys Lys Val Lys Gly Thr Ala 
785                 790                 795                 800 


Lys Val Tyr Val Gly Leu Arg Phe Ala Ile Ser Gln His Ile Arg Asp 
                805                 810                 815     


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Ser Ile 
            820                 825                 830         


Arg Glu Lys Asn Lys Ser Glu Phe Arg Trp Leu Glu Phe Glu Val Thr 
        835                 840                 845             


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
    850                 855                 860                 


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
865                 870                 875                 880 


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
                885                 890                 895     


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg 
            900                 905                 


<210>  11
<211>  3372
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-Trex2 DNA construct

<400>  11
atgggatcct gcaggccacc taagaagaaa cgcaaagtcg tggatctacg cacgctcggc       60

tacagtcagc agcagcaaga gaagatcaaa ccgaaggtgc gttcgacagt ggcgcagcac      120

cacgaggcac tggtgggcca tgggtttaca cacgcgcaca tcgttgcgct cagccaacac      180

ccggcagcgt tagggaccgt cgctgtcacg tatcagcaca taatcacggc gttgccagag      240

gcgacacacg aagacatcgt tggcgtcggc aaacagtggt ccggcgcacg cgccctggag      300

gccttgctca cggatgcggg ggagttgaga ggtccgccgt tacagttgga cacaggccaa      360

cttgtgaaga ttgcaaaacg tggcggcgtg accgcaatgg aggcagtgca tgcatcgcgc      420

aatgcactga cgggtgcccc cctgaacctg accccggacc aagtggtggc tatcgccagc      480

aacattggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag      540

gaccatggcc tgaccccgga ccaagtggtg gctatcgcca gcaacattgg cggcaagcaa      600

gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg      660

gaccaagtgg tggctatcgc cagcaacatt ggcggcaagc aagcgctcga aacggtgcag      720

cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc      780

gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg      840

tgccaggacc atggcctgac tccggaccaa gtggtggcta tcgccagcca cgatggcggc      900

aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg      960

actccggacc aagtggtggc tatcgccagc cacgatggcg gcaagcaagc gctcgaaacg     1020

gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg     1080

gctatcgcca gcaacattgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg     1140

gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc cagcaacaat     1200

ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat     1260

ggcctgaccc cggaccaagt ggtggctatc gccagcaacg gtggcggcaa gcaagcgctc     1320

gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa     1380

gtggtggcta tcgccagcaa caatggcggc aagcaagcgc tcgaaacggt gcagcggctg     1440

ttgccggtgc tgtgccagga ccatggcctg accccggacc aagtggtggc tatcgccagc     1500

aacattggcg gcaagcaagc gctcgaaagc attgtggccc agctgagccg gcctgatccg     1560

gcgttggccg cgttgaccaa cgaccacctc gtcgccttgg cctgcctcgg cggacgtcct     1620

gccatggatg cagtgaaaaa gggattgccg cacgcgccgg aattgatcag aagagtcaat     1680

cgccgtattg gcgaacgcac gtcccatcgc gttgcgatat ctagagtggg aggaagctct     1740

cgcagagagt ccatcaaccc atggattctg actggtttcg ctgatgccga aggatcattc     1800

atactagaca tccgcaaccg aaacaacgaa agcaacagat accgaacttc gctgagattc     1860

cagatcaccc tgcacaacaa ggacaaatcg attctggaga atatccagtc cacttggaag     1920

gtcggcaaga tcacaaacag cagtgacaga gccgtcatgc tgagggtcac ccgtttcgaa     1980

gatttgaaag tgattatcga ccacttcgag aaatatccgc tgattaccca gaaattgggc     2040

gattacaagt tgtttaaaca ggcattcagc gtcatggaga ataaagaaca tcttaaggag     2100

aatgggatta aggagctcgt acgaatcaaa gctaagatga attggggtct caatgacgaa     2160

ttgaaaaaag catttccaga gaacattagc aaagagcgcc cccttatcaa taagaacatt     2220

ccgaatttca aatggctggc tggattcaca gctggtgaag gctacttcgg cgtgaatcta     2280

aaaaaggtaa agggcaccgc aaaggtatac gtgggactga gattctcaat ctcacagcac     2340

atcagagaca agaacctgat gaattcattg ataacatacc taggctgtgg ttccatctgg     2400

gagaagaaca agtctgagtt cagatggctc gagttcgtcg taaccaaatt cagcgatatc     2460

aacgacaaga tcattccggt attccaggaa aatactctga ttggcgtcaa actcgaggac     2520

tttgaagatt ggtgcaaggt tgccaaattg atcaaagaga agaaacacct gaccgaatcc     2580

ggtttggatg agattaagaa aatcaagctg aacatgaaca aaggtcgtgg tggaggcggt     2640

agcggaggcg gagggtcggc tagctccgaa gcaccgagag ccgaaacctt cgtgttcctc     2700

gacctggaag ccactggcct gcccagtgtg gagcccgaga ttgccgagct gtccctgttc     2760

gccgttcatc ggtcctccct ggaaaaccct gagcacgacg agagcggagc actggtgctg     2820

ccaagggtgc tggacaagct gaccctgtgc atgtgtcccg agcgtccctt cactgccaag     2880

gcatccgaaa tcactggtct gagttcggaa gggctggctc gttgtagaaa ggctggattc     2940

gatggtgccg tggttcggac tttgcaggca ttcctgtcca ggcaggcagg tccaatctgc     3000

ttggtcgccc acaacggttt tgactacgac ttcccactgc tctgtgccga actgcgtcgc     3060

ctgggagcac gccttcctcg ggataccgtg tgcctggaca ccctacccgc tctgcgtgga     3120

ctggacagag cccactcaca cggcactagg gcgagaggac gccagggtta cagcctcggt     3180

tcactgttcc atcggtattt cagggcagag ccttccgcag cccattcagc cgaaggcgat     3240

gtccacacct tgcttctgat cttcctgcat cgtgcagcag aactgctcgc ctgggcagat     3300

gagcaggctc gtggatgggc tcacattgag ccgatgtact tgccacctga tgaccctagc     3360

ctggaagcct ga                                                         3372


<210>  12
<211>  3372
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-Trex2 RNA construct

<400>  12
augggauccu gcaggccacc uaagaagaaa cgcaaagucg uggaucuacg cacgcucggc       60

uacagucagc agcagcaaga gaagaucaaa ccgaaggugc guucgacagu ggcgcagcac      120

cacgaggcac uggugggcca uggguuuaca cacgcgcaca ucguugcgcu cagccaacac      180

ccggcagcgu uagggaccgu cgcugucacg uaucagcaca uaaucacggc guugccagag      240

gcgacacacg aagacaucgu uggcgucggc aaacaguggu ccggcgcacg cgcccuggag      300

gccuugcuca cggaugcggg ggaguugaga gguccgccgu uacaguugga cacaggccaa      360

cuugugaaga uugcaaaacg uggcggcgug accgcaaugg aggcagugca ugcaucgcgc      420

aaugcacuga cgggugcccc ccugaaccug accccggacc aagugguggc uaucgccagc      480

aacauuggcg gcaagcaagc gcucgaaacg gugcagcggc uguugccggu gcugugccag      540

gaccauggcc ugaccccgga ccaaguggug gcuaucgcca gcaacauugg cggcaagcaa      600

gcgcucgaaa cggugcagcg gcuguugccg gugcugugcc aggaccaugg ccugaccccg      660

gaccaagugg uggcuaucgc cagcaacauu ggcggcaagc aagcgcucga aacggugcag      720

cggcuguugc cggugcugug ccaggaccau ggccugaccc cggaccaagu gguggcuauc      780

gccagcaacg guggcggcaa gcaagcgcuc gaaacggugc agcggcuguu gccggugcug      840

ugccaggacc auggccugac uccggaccaa gugguggcua ucgccagcca cgauggcggc      900

aagcaagcgc ucgaaacggu gcagcggcug uugccggugc ugugccagga ccauggccug      960

acuccggacc aagugguggc uaucgccagc cacgauggcg gcaagcaagc gcucgaaacg     1020

gugcagcggc uguugccggu gcugugccag gaccauggcc ugaccccgga ccaaguggug     1080

gcuaucgcca gcaacauugg cggcaagcaa gcgcucgaaa cggugcagcg gcuguugccg     1140

gugcugugcc aggaccaugg ccugaccccg gaccaagugg uggcuaucgc cagcaacaau     1200

ggcggcaagc aagcgcucga aacggugcag cggcuguugc cggugcugug ccaggaccau     1260

ggccugaccc cggaccaagu gguggcuauc gccagcaacg guggcggcaa gcaagcgcuc     1320

gaaacggugc agcggcuguu gccggugcug ugccaggacc auggccugac cccggaccaa     1380

gugguggcua ucgccagcaa caauggcggc aagcaagcgc ucgaaacggu gcagcggcug     1440

uugccggugc ugugccagga ccauggccug accccggacc aagugguggc uaucgccagc     1500

aacauuggcg gcaagcaagc gcucgaaagc auuguggccc agcugagccg gccugauccg     1560

gcguuggccg cguugaccaa cgaccaccuc gucgccuugg ccugccucgg cggacguccu     1620

gccauggaug cagugaaaaa gggauugccg cacgcgccgg aauugaucag aagagucaau     1680

cgccguauug gcgaacgcac gucccaucgc guugcgauau cuagaguggg aggaagcucu     1740

cgcagagagu ccaucaaccc auggauucug acugguuucg cugaugccga aggaucauuc     1800

auacuagaca uccgcaaccg aaacaacgaa agcaacagau accgaacuuc gcugagauuc     1860

cagaucaccc ugcacaacaa ggacaaaucg auucuggaga auauccaguc cacuuggaag     1920

gucggcaaga ucacaaacag cagugacaga gccgucaugc ugagggucac ccguuucgaa     1980

gauuugaaag ugauuaucga ccacuucgag aaauauccgc ugauuaccca gaaauugggc     2040

gauuacaagu uguuuaaaca ggcauucagc gucauggaga auaaagaaca ucuuaaggag     2100

aaugggauua aggagcucgu acgaaucaaa gcuaagauga auuggggucu caaugacgaa     2160

uugaaaaaag cauuuccaga gaacauuagc aaagagcgcc cccuuaucaa uaagaacauu     2220

ccgaauuuca aauggcuggc uggauucaca gcuggugaag gcuacuucgg cgugaaucua     2280

aaaaagguaa agggcaccgc aaagguauac gugggacuga gauucucaau cucacagcac     2340

aucagagaca agaaccugau gaauucauug auaacauacc uaggcugugg uuccaucugg     2400

gagaagaaca agucugaguu cagauggcuc gaguucgucg uaaccaaauu cagcgauauc     2460

aacgacaaga ucauuccggu auuccaggaa aauacucuga uuggcgucaa acucgaggac     2520

uuugaagauu ggugcaaggu ugccaaauug aucaaagaga agaaacaccu gaccgaaucc     2580

gguuuggaug agauuaagaa aaucaagcug aacaugaaca aaggucgugg uggaggcggu     2640

agcggaggcg gagggucggc uagcuccgaa gcaccgagag ccgaaaccuu cguguuccuc     2700

gaccuggaag ccacuggccu gcccagugug gagcccgaga uugccgagcu gucccuguuc     2760

gccguucauc gguccucccu ggaaaacccu gagcacgacg agagcggagc acuggugcug     2820

ccaagggugc uggacaagcu gacccugugc augugucccg agcgucccuu cacugccaag     2880

gcauccgaaa ucacuggucu gaguucggaa gggcuggcuc guuguagaaa ggcuggauuc     2940

gauggugccg ugguucggac uuugcaggca uuccugucca ggcaggcagg uccaaucugc     3000

uuggucgccc acaacgguuu ugacuacgac uucccacugc ucugugccga acugcgucgc     3060

cugggagcac gccuuccucg ggauaccgug ugccuggaca cccuacccgc ucugcgugga     3120

cuggacagag cccacucaca cggcacuagg gcgagaggac gccaggguua cagccucggu     3180

ucacuguucc aucgguauuu cagggcagag ccuuccgcag cccauucagc cgaaggcgau     3240

guccacaccu ugcuucugau cuuccugcau cgugcagcag aacugcucgc cugggcagau     3300

gagcaggcuc guggaugggc ucacauugag ccgauguacu ugccaccuga ugacccuagc     3360

cuggaagccu ga                                                         3372


<210>  13
<211>  1123
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-Trex2 protein 
       construct

<400>  13

Met Gly Ser Cys Arg Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser Ile Val 
            500                 505                 510         


Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp 
        515                 520                 525             


His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala 
    530                 535                 540                 


Val Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn 
545                 550                 555                 560 


Arg Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val 
                565                 570                 575     


Gly Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly 
            580                 585                 590         


Phe Ala Asp Ala Glu Gly Ser Phe Ile Leu Asp Ile Arg Asn Arg Asn 
        595                 600                 605             


Asn Glu Ser Asn Arg Tyr Arg Thr Ser Leu Arg Phe Gln Ile Thr Leu 
    610                 615                 620                 


His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys 
625                 630                 635                 640 


Val Gly Lys Ile Thr Asn Ser Ser Asp Arg Ala Val Met Leu Arg Val 
                645                 650                 655     


Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr 
            660                 665                 670         


Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala 
        675                 680                 685             


Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys 
    690                 695                 700                 


Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp Glu 
705                 710                 715                 720 


Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile 
                725                 730                 735     


Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ala Gly 
            740                 745                 750         


Glu Gly Tyr Phe Gly Val Asn Leu Lys Lys Val Lys Gly Thr Ala Lys 
        755                 760                 765             


Val Tyr Val Gly Leu Arg Phe Ser Ile Ser Gln His Ile Arg Asp Lys 
    770                 775                 780                 


Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Ser Ile Trp 
785                 790                 795                 800 


Glu Lys Asn Lys Ser Glu Phe Arg Trp Leu Glu Phe Val Val Thr Lys 
                805                 810                 815     


Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr 
            820                 825                 830         


Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala 
        835                 840                 845             


Lys Leu Ile Lys Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu 
    850                 855                 860                 


Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Gly Gly Gly Gly 
865                 870                 875                 880 


Ser Gly Gly Gly Gly Ser Ala Ser Ser Glu Ala Pro Arg Ala Glu Thr 
                885                 890                 895     


Phe Val Phe Leu Asp Leu Glu Ala Thr Gly Leu Pro Ser Val Glu Pro 
            900                 905                 910         


Glu Ile Ala Glu Leu Ser Leu Phe Ala Val His Arg Ser Ser Leu Glu 
        915                 920                 925             


Asn Pro Glu His Asp Glu Ser Gly Ala Leu Val Leu Pro Arg Val Leu 
    930                 935                 940                 


Asp Lys Leu Thr Leu Cys Met Cys Pro Glu Arg Pro Phe Thr Ala Lys 
945                 950                 955                 960 


Ala Ser Glu Ile Thr Gly Leu Ser Ser Glu Gly Leu Ala Arg Cys Arg 
                965                 970                 975     


Lys Ala Gly Phe Asp Gly Ala Val Val Arg Thr Leu Gln Ala Phe Leu 
            980                 985                 990         


Ser Arg Gln Ala Gly Pro Ile Cys  Leu Val Ala His Asn  Gly Phe Asp 
        995                 1000                 1005             


Tyr Asp  Phe Pro Leu Leu Cys  Ala Glu Leu Arg Arg  Leu Gly Ala 
    1010                 1015                 1020             


Arg Leu  Pro Arg Asp Thr Val  Cys Leu Asp Thr Leu  Pro Ala Leu 
    1025                 1030                 1035             


Arg Gly  Leu Asp Arg Ala His  Ser His Gly Thr Arg  Ala Arg Gly 
    1040                 1045                 1050             


Arg Gln  Gly Tyr Ser Leu Gly  Ser Leu Phe His Arg  Tyr Phe Arg 
    1055                 1060                 1065             


Ala Glu  Pro Ser Ala Ala His  Ser Ala Glu Gly Asp  Val His Thr 
    1070                 1075                 1080             


Leu Leu  Leu Ile Phe Leu His  Arg Ala Ala Glu Leu  Leu Ala Trp 
    1085                 1090                 1095             


Ala Asp  Glu Gln Ala Arg Gly  Trp Ala His Ile Glu  Pro Met Tyr 
    1100                 1105                 1110             


Leu Pro  Pro Asp Asp Pro Ser  Leu Glu Ala 
    1115                 1120             


<210>  14
<211>  747
<212>  DNA
<213>  Homo sapiens

<400>  14
atgggatcct gcaggccacc taagaagaaa cgcaaagtct ccgaagcacc gagagccgaa       60

accttcgtgt tcctcgacct ggaagccact ggcctgccca gtgtggagcc cgagattgcc      120

gagctgtccc tgttcgccgt tcatcggtcc tccctggaaa accctgagca cgacgagagc      180

ggagcactgg tgctgccaag ggtgctggac aagctgaccc tgtgcatgtg tcccgagcgt      240

cccttcactg ccaaggcatc cgaaatcact ggtctgagtt cggaagggct ggctcgttgt      300

agaaaggctg gattcgatgg tgccgtggtt cggactttgc aggcattcct gtccaggcag      360

gcaggtccaa tctgcttggt cgcccacaac ggttttgact acgacttccc actgctctgt      420

gccgaactgc gtcgcctggg agcacgcctt cctcgggata ccgtgtgcct ggacacccta      480

cccgctctgc gtggactgga cagagcccac tcacacggca ctagggcgag aggacgccag      540

ggttacagcc tcggttcact gttccatcgg tatttcaggg cagagccttc cgcagcccat      600

tcagccgaag gcgatgtcca caccttgctt ctgatcttcc tgcatcgtgc agcagaactg      660

ctcgcctggg cagatgagca ggctcgtgga tgggctcaca ttgagccgat gtacttgcca      720

cctgatgacc ctagcctgga agcctga                                          747


<210>  15
<211>  747
<212>  RNA
<213>  Homo sapiens

<400>  15
augggauccu gcaggccacc uaagaagaaa cgcaaagucu ccgaagcacc gagagccgaa       60

accuucgugu uccucgaccu ggaagccacu ggccugccca guguggagcc cgagauugcc      120

gagcuguccc uguucgccgu ucaucggucc ucccuggaaa acccugagca cgacgagagc      180

ggagcacugg ugcugccaag ggugcuggac aagcugaccc ugugcaugug ucccgagcgu      240

cccuucacug ccaaggcauc cgaaaucacu ggucugaguu cggaagggcu ggcucguugu      300

agaaaggcug gauucgaugg ugccgugguu cggacuuugc aggcauuccu guccaggcag      360

gcagguccaa ucugcuuggu cgcccacaac gguuuugacu acgacuuccc acugcucugu      420

gccgaacugc gucgccuggg agcacgccuu ccucgggaua ccgugugccu ggacacccua      480

cccgcucugc guggacugga cagagcccac ucacacggca cuagggcgag aggacgccag      540

gguuacagcc ucgguucacu guuccaucgg uauuucaggg cagagccuuc cgcagcccau      600

ucagccgaag gcgaugucca caccuugcuu cugaucuucc ugcaucgugc agcagaacug      660

cucgccuggg cagaugagca ggcucgugga ugggcucaca uugagccgau guacuugcca      720

ccugaugacc cuagccugga agccuga                                          747


<210>  16
<211>  248
<212>  PRT
<213>  Homo sapiens

<400>  16

Met Gly Ser Cys Arg Pro Pro Lys Lys Lys Arg Lys Val Ser Glu Ala 
1               5                   10                  15      


Pro Arg Ala Glu Thr Phe Val Phe Leu Asp Leu Glu Ala Thr Gly Leu 
            20                  25                  30          


Pro Ser Val Glu Pro Glu Ile Ala Glu Leu Ser Leu Phe Ala Val His 
        35                  40                  45              


Arg Ser Ser Leu Glu Asn Pro Glu His Asp Glu Ser Gly Ala Leu Val 
    50                  55                  60                  


Leu Pro Arg Val Leu Asp Lys Leu Thr Leu Cys Met Cys Pro Glu Arg 
65                  70                  75                  80  


Pro Phe Thr Ala Lys Ala Ser Glu Ile Thr Gly Leu Ser Ser Glu Gly 
                85                  90                  95      


Leu Ala Arg Cys Arg Lys Ala Gly Phe Asp Gly Ala Val Val Arg Thr 
            100                 105                 110         


Leu Gln Ala Phe Leu Ser Arg Gln Ala Gly Pro Ile Cys Leu Val Ala 
        115                 120                 125             


His Asn Gly Phe Asp Tyr Asp Phe Pro Leu Leu Cys Ala Glu Leu Arg 
    130                 135                 140                 


Arg Leu Gly Ala Arg Leu Pro Arg Asp Thr Val Cys Leu Asp Thr Leu 
145                 150                 155                 160 


Pro Ala Leu Arg Gly Leu Asp Arg Ala His Ser His Gly Thr Arg Ala 
                165                 170                 175     


Arg Gly Arg Gln Gly Tyr Ser Leu Gly Ser Leu Phe His Arg Tyr Phe 
            180                 185                 190         


Arg Ala Glu Pro Ser Ala Ala His Ser Ala Glu Gly Asp Val His Thr 
        195                 200                 205             


Leu Leu Leu Ile Phe Leu His Arg Ala Ala Glu Leu Leu Ala Trp Ala 
    210                 215                 220                 


Asp Glu Gln Ala Arg Gly Trp Ala His Ile Glu Pro Met Tyr Leu Pro 
225                 230                 235                 240 


Pro Asp Asp Pro Ser Leu Glu Ala 
                245             


<210>  17
<211>  3384
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-Trex2platypus DNA 
       construct

<400>  17
atgggatcct gcaggccacc taagaagaaa cgcaaagtcg tggatctacg cacgctcggc       60

tacagtcagc agcagcaaga gaagatcaaa ccgaaggtgc gttcgacagt ggcgcagcac      120

cacgaggcac tggtgggcca tgggtttaca cacgcgcaca tcgttgcgct cagccaacac      180

ccggcagcgt tagggaccgt cgctgtcacg tatcagcaca taatcacggc gttgccagag      240

gcgacacacg aagacatcgt tggcgtcggc aaacagtggt ccggcgcacg cgccctggag      300

gccttgctca cggatgcggg ggagttgaga ggtccgccgt tacagttgga cacaggccaa      360

cttgtgaaga ttgcaaaacg tggcggcgtg accgcaatgg aggcagtgca tgcatcgcgc      420

aatgcactga cgggtgcccc cctgaacctg accccggacc aagtggtggc tatcgccagc      480

aacattggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag      540

gaccatggcc tgaccccgga ccaagtggtg gctatcgcca gcaacattgg cggcaagcaa      600

gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg      660

gaccaagtgg tggctatcgc cagcaacatt ggcggcaagc aagcgctcga aacggtgcag      720

cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc      780

gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg      840

tgccaggacc atggcctgac tccggaccaa gtggtggcta tcgccagcca cgatggcggc      900

aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg      960

actccggacc aagtggtggc tatcgccagc cacgatggcg gcaagcaagc gctcgaaacg     1020

gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg     1080

gctatcgcca gcaacattgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg     1140

gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc cagcaacaat     1200

ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat     1260

ggcctgaccc cggaccaagt ggtggctatc gccagcaacg gtggcggcaa gcaagcgctc     1320

gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa     1380

gtggtggcta tcgccagcaa caatggcggc aagcaagcgc tcgaaacggt gcagcggctg     1440

ttgccggtgc tgtgccagga ccatggcctg accccggacc aagtggtggc tatcgccagc     1500

aacattggcg gcaagcaagc gctcgaaagc attgtggccc agctgagccg gcctgatccg     1560

gcgttggccg cgttgaccaa cgaccacctc gtcgccttgg cctgcctcgg cggacgtcct     1620

gccatggatg cagtgaaaaa gggattgccg cacgcgccgg aattgatcag aagagtcaat     1680

cgccgtattg gcgaacgcac gtcccatcgc gttgcgatat ctagagtggg aggaagctct     1740

cgcagagagt ccatcaaccc atggattctg actggtttcg ctgatgccga aggatcattc     1800

atactagaca tccgcaaccg aaacaacgaa agcaacagat accgaacttc gctgagattc     1860

cagatcaccc tgcacaacaa ggacaaatcg attctggaga atatccagtc cacttggaag     1920

gtcggcaaga tcacaaacag cagtgacaga gccgtcatgc tgagggtcac ccgtttcgaa     1980

gatttgaaag tgattatcga ccacttcgag aaatatccgc tgattaccca gaaattgggc     2040

gattacaagt tgtttaaaca ggcattcagc gtcatggaga ataaagaaca tcttaaggag     2100

aatgggatta aggagctcgt acgaatcaaa gctaagatga attggggtct caatgacgaa     2160

ttgaaaaaag catttccaga gaacattagc aaagagcgcc cccttatcaa taagaacatt     2220

ccgaatttca aatggctggc tggattcaca gctggtgaag gctacttcgg cgtgaatcta     2280

aaaaaggtaa agggcaccgc aaaggtatac gtgggactga gattctcaat ctcacagcac     2340

atcagagaca agaacctgat gaattcattg ataacatacc taggctgtgg ttccatctgg     2400

gagaagaaca agtctgagtt cagatggctc gagttcgtcg taaccaaatt cagcgatatc     2460

aacgacaaga tcattccggt attccaggaa aatactctga ttggcgtcaa actcgaggac     2520

tttgaagatt ggtgcaaggt tgccaaattg atcaaagaga agaaacacct gaccgaatcc     2580

ggtttggatg agattaagaa aatcaagctg aacatgaaca aaggtcgtgg tggaggcggt     2640

agcggaggcg gagggtcggc tagctccgaa tcacagaaat gcgaaacctt cgtgttcctc     2700

gacctggaag ccactggcct gcccgatgcg tctccccaga ttgccgaaat ctccctgttc     2760

gccgttcatc ggttctccct ggaacaccct gcgtgggacg atagcggagt accgctgctg     2820

ccaagggtgc tggacaagct gaccctgtgc atgaatcccg agcaaccctt cactcccaag     2880

gcagccaaga tcactggtct gaataacaga tccctgactg ataatagaaa ggcttgtttc     2940

gatgggaccg tggttaaggc tttgcgggag ttcctgtcca ggcaggcaag tccaatctgc     3000

ttggtcgccc acaacggttt tgcctacgac ttcccactgc tcaggaccga actgggtcgc     3060

ctggatgcaa accttcctga gaatacctat tgcctggaca ccctacccgc tctgaaagga     3120

ctggacaaag cccacgatca cggcactagg tcgaatcatg gcaagtctta ccgcctcggt     3180

gatctctaca ggcagtattt cggtgacgat cctaaagcag cccattcagc cgaaggcgat     3240

gtctacacct tggttatggt cttcctgcat cgggcaccag aactgctccg ttgggcaggt     3300

gcggaggctc gttcatggga tgagattagg ccgatgtact ccccaggtcc tcgtagatca     3360

ggtggcagaa ggcatgatgc ctga                                            3384


<210>  18
<211>  3384
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-Trex2platypus RNA 
       construct

<400>  18
augggauccu gcaggccacc uaagaagaaa cgcaaagucg uggaucuacg cacgcucggc       60

uacagucagc agcagcaaga gaagaucaaa ccgaaggugc guucgacagu ggcgcagcac      120

cacgaggcac uggugggcca uggguuuaca cacgcgcaca ucguugcgcu cagccaacac      180

ccggcagcgu uagggaccgu cgcugucacg uaucagcaca uaaucacggc guugccagag      240

gcgacacacg aagacaucgu uggcgucggc aaacaguggu ccggcgcacg cgcccuggag      300

gccuugcuca cggaugcggg ggaguugaga gguccgccgu uacaguugga cacaggccaa      360

cuugugaaga uugcaaaacg uggcggcgug accgcaaugg aggcagugca ugcaucgcgc      420

aaugcacuga cgggugcccc ccugaaccug accccggacc aagugguggc uaucgccagc      480

aacauuggcg gcaagcaagc gcucgaaacg gugcagcggc uguugccggu gcugugccag      540

gaccauggcc ugaccccgga ccaaguggug gcuaucgcca gcaacauugg cggcaagcaa      600

gcgcucgaaa cggugcagcg gcuguugccg gugcugugcc aggaccaugg ccugaccccg      660

gaccaagugg uggcuaucgc cagcaacauu ggcggcaagc aagcgcucga aacggugcag      720

cggcuguugc cggugcugug ccaggaccau ggccugaccc cggaccaagu gguggcuauc      780

gccagcaacg guggcggcaa gcaagcgcuc gaaacggugc agcggcuguu gccggugcug      840

ugccaggacc auggccugac uccggaccaa gugguggcua ucgccagcca cgauggcggc      900

aagcaagcgc ucgaaacggu gcagcggcug uugccggugc ugugccagga ccauggccug      960

acuccggacc aagugguggc uaucgccagc cacgauggcg gcaagcaagc gcucgaaacg     1020

gugcagcggc uguugccggu gcugugccag gaccauggcc ugaccccgga ccaaguggug     1080

gcuaucgcca gcaacauugg cggcaagcaa gcgcucgaaa cggugcagcg gcuguugccg     1140

gugcugugcc aggaccaugg ccugaccccg gaccaagugg uggcuaucgc cagcaacaau     1200

ggcggcaagc aagcgcucga aacggugcag cggcuguugc cggugcugug ccaggaccau     1260

ggccugaccc cggaccaagu gguggcuauc gccagcaacg guggcggcaa gcaagcgcuc     1320

gaaacggugc agcggcuguu gccggugcug ugccaggacc auggccugac cccggaccaa     1380

gugguggcua ucgccagcaa caauggcggc aagcaagcgc ucgaaacggu gcagcggcug     1440

uugccggugc ugugccagga ccauggccug accccggacc aagugguggc uaucgccagc     1500

aacauuggcg gcaagcaagc gcucgaaagc auuguggccc agcugagccg gccugauccg     1560

gcguuggccg cguugaccaa cgaccaccuc gucgccuugg ccugccucgg cggacguccu     1620

gccauggaug cagugaaaaa gggauugccg cacgcgccgg aauugaucag aagagucaau     1680

cgccguauug gcgaacgcac gucccaucgc guugcgauau cuagaguggg aggaagcucu     1740

cgcagagagu ccaucaaccc auggauucug acugguuucg cugaugccga aggaucauuc     1800

auacuagaca uccgcaaccg aaacaacgaa agcaacagau accgaacuuc gcugagauuc     1860

cagaucaccc ugcacaacaa ggacaaaucg auucuggaga auauccaguc cacuuggaag     1920

gucggcaaga ucacaaacag cagugacaga gccgucaugc ugagggucac ccguuucgaa     1980

gauuugaaag ugauuaucga ccacuucgag aaauauccgc ugauuaccca gaaauugggc     2040

gauuacaagu uguuuaaaca ggcauucagc gucauggaga auaaagaaca ucuuaaggag     2100

aaugggauua aggagcucgu acgaaucaaa gcuaagauga auuggggucu caaugacgaa     2160

uugaaaaaag cauuuccaga gaacauuagc aaagagcgcc cccuuaucaa uaagaacauu     2220

ccgaauuuca aauggcuggc uggauucaca gcuggugaag gcuacuucgg cgugaaucua     2280

aaaaagguaa agggcaccgc aaagguauac gugggacuga gauucucaau cucacagcac     2340

aucagagaca agaaccugau gaauucauug auaacauacc uaggcugugg uuccaucugg     2400

gagaagaaca agucugaguu cagauggcuc gaguucgucg uaaccaaauu cagcgauauc     2460

aacgacaaga ucauuccggu auuccaggaa aauacucuga uuggcgucaa acucgaggac     2520

uuugaagauu ggugcaaggu ugccaaauug aucaaagaga agaaacaccu gaccgaaucc     2580

gguuuggaug agauuaagaa aaucaagcug aacaugaaca aaggucgugg uggaggcggu     2640

agcggaggcg gagggucggc uagcuccgaa ucacagaaau gcgaaaccuu cguguuccuc     2700

gaccuggaag ccacuggccu gcccgaugcg ucuccccaga uugccgaaau cucccuguuc     2760

gccguucauc gguucucccu ggaacacccu gcgugggacg auagcggagu accgcugcug     2820

ccaagggugc uggacaagcu gacccugugc augaaucccg agcaacccuu cacucccaag     2880

gcagccaaga ucacuggucu gaauaacaga ucccugacug auaauagaaa ggcuuguuuc     2940

gaugggaccg ugguuaaggc uuugcgggag uuccugucca ggcaggcaag uccaaucugc     3000

uuggucgccc acaacgguuu ugccuacgac uucccacugc ucaggaccga acugggucgc     3060

cuggaugcaa accuuccuga gaauaccuau ugccuggaca cccuacccgc ucugaaagga     3120

cuggacaaag cccacgauca cggcacuagg ucgaaucaug gcaagucuua ccgccucggu     3180

gaucucuaca ggcaguauuu cggugacgau ccuaaagcag cccauucagc cgaaggcgau     3240

gucuacaccu ugguuauggu cuuccugcau cgggcaccag aacugcuccg uugggcaggu     3300

gcggaggcuc guucauggga ugagauuagg ccgauguacu ccccaggucc ucguagauca     3360

gguggcagaa ggcaugaugc cuga                                            3384


<210>  19
<211>  1127
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-Trex2platypus protein
       construct

<400>  19

Met Gly Ser Cys Arg Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser Ile Val 
            500                 505                 510         


Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp 
        515                 520                 525             


His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala 
    530                 535                 540                 


Val Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn 
545                 550                 555                 560 


Arg Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val 
                565                 570                 575     


Gly Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly 
            580                 585                 590         


Phe Ala Asp Ala Glu Gly Ser Phe Ile Leu Asp Ile Arg Asn Arg Asn 
        595                 600                 605             


Asn Glu Ser Asn Arg Tyr Arg Thr Ser Leu Arg Phe Gln Ile Thr Leu 
    610                 615                 620                 


His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys 
625                 630                 635                 640 


Val Gly Lys Ile Thr Asn Ser Ser Asp Arg Ala Val Met Leu Arg Val 
                645                 650                 655     


Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr 
            660                 665                 670         


Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala 
        675                 680                 685             


Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys 
    690                 695                 700                 


Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp Glu 
705                 710                 715                 720 


Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile 
                725                 730                 735     


Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ala Gly 
            740                 745                 750         


Glu Gly Tyr Phe Gly Val Asn Leu Lys Lys Val Lys Gly Thr Ala Lys 
        755                 760                 765             


Val Tyr Val Gly Leu Arg Phe Ser Ile Ser Gln His Ile Arg Asp Lys 
    770                 775                 780                 


Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Ser Ile Trp 
785                 790                 795                 800 


Glu Lys Asn Lys Ser Glu Phe Arg Trp Leu Glu Phe Val Val Thr Lys 
                805                 810                 815     


Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr 
            820                 825                 830         


Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala 
        835                 840                 845             


Lys Leu Ile Lys Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu 
    850                 855                 860                 


Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Gly Gly Gly Gly 
865                 870                 875                 880 


Ser Gly Gly Gly Gly Ser Ala Ser Ser Glu Ser Gln Lys Cys Glu Thr 
                885                 890                 895     


Phe Val Phe Leu Asp Leu Glu Ala Thr Gly Leu Pro Asp Ala Ser Pro 
            900                 905                 910         


Gln Ile Ala Glu Ile Ser Leu Phe Ala Val His Arg Phe Ser Leu Glu 
        915                 920                 925             


His Pro Ala Trp Asp Asp Ser Gly Val Pro Leu Leu Pro Arg Val Leu 
    930                 935                 940                 


Asp Lys Leu Thr Leu Cys Met Asn Pro Glu Gln Pro Phe Thr Pro Lys 
945                 950                 955                 960 


Ala Ala Lys Ile Thr Gly Leu Asn Asn Arg Ser Leu Thr Asp Asn Arg 
                965                 970                 975     


Lys Ala Cys Phe Asp Gly Thr Val Val Lys Ala Leu Arg Glu Phe Leu 
            980                 985                 990         


Ser Arg Gln Ala Ser Pro Ile Cys  Leu Val Ala His Asn  Gly Phe Ala 
        995                 1000                 1005             


Tyr Asp  Phe Pro Leu Leu Arg  Thr Glu Leu Gly Arg  Leu Asp Ala 
    1010                 1015                 1020             


Asn Leu  Pro Glu Asn Thr Tyr  Cys Leu Asp Thr Leu  Pro Ala Leu 
    1025                 1030                 1035             


Lys Gly  Leu Asp Lys Ala His  Asp His Gly Thr Arg  Ser Asn His 
    1040                 1045                 1050             


Gly Lys  Ser Tyr Arg Leu Gly  Asp Leu Tyr Arg Gln  Tyr Phe Gly 
    1055                 1060                 1065             


Asp Asp  Pro Lys Ala Ala His  Ser Ala Glu Gly Asp  Val Tyr Thr 
    1070                 1075                 1080             


Leu Val  Met Val Phe Leu His  Arg Ala Pro Glu Leu  Leu Arg Trp 
    1085                 1090                 1095             


Ala Gly  Ala Glu Ala Arg Ser  Trp Asp Glu Ile Arg  Pro Met Tyr 
    1100                 1105                 1110             


Ser Pro  Gly Pro Arg Arg Ser  Gly Gly Arg Arg His  Asp Ala 
    1115                 1120                 1125         


<210>  20
<211>  3387
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-Trex2opossum DNA 
       construct

<400>  20
atgggatcct gcaggccacc taagaagaaa cgcaaagtcg tggatctacg cacgctcggc       60

tacagtcagc agcagcaaga gaagatcaaa ccgaaggtgc gttcgacagt ggcgcagcac      120

cacgaggcac tggtgggcca tgggtttaca cacgcgcaca tcgttgcgct cagccaacac      180

ccggcagcgt tagggaccgt cgctgtcacg tatcagcaca taatcacggc gttgccagag      240

gcgacacacg aagacatcgt tggcgtcggc aaacagtggt ccggcgcacg cgccctggag      300

gccttgctca cggatgcggg ggagttgaga ggtccgccgt tacagttgga cacaggccaa      360

cttgtgaaga ttgcaaaacg tggcggcgtg accgcaatgg aggcagtgca tgcatcgcgc      420

aatgcactga cgggtgcccc cctgaacctg accccggacc aagtggtggc tatcgccagc      480

aacattggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag      540

gaccatggcc tgaccccgga ccaagtggtg gctatcgcca gcaacattgg cggcaagcaa      600

gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg      660

gaccaagtgg tggctatcgc cagcaacatt ggcggcaagc aagcgctcga aacggtgcag      720

cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc      780

gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg      840

tgccaggacc atggcctgac tccggaccaa gtggtggcta tcgccagcca cgatggcggc      900

aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg      960

actccggacc aagtggtggc tatcgccagc cacgatggcg gcaagcaagc gctcgaaacg     1020

gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg     1080

gctatcgcca gcaacattgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg     1140

gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc cagcaacaat     1200

ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat     1260

ggcctgaccc cggaccaagt ggtggctatc gccagcaacg gtggcggcaa gcaagcgctc     1320

gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa     1380

gtggtggcta tcgccagcaa caatggcggc aagcaagcgc tcgaaacggt gcagcggctg     1440

ttgccggtgc tgtgccagga ccatggcctg accccggacc aagtggtggc tatcgccagc     1500

aacattggcg gcaagcaagc gctcgaaagc attgtggccc agctgagccg gcctgatccg     1560

gcgttggccg cgttgaccaa cgaccacctc gtcgccttgg cctgcctcgg cggacgtcct     1620

gccatggatg cagtgaaaaa gggattgccg cacgcgccgg aattgatcag aagagtcaat     1680

cgccgtattg gcgaacgcac gtcccatcgc gttgcgatat ctagagtggg aggaagctct     1740

cgcagagagt ccatcaaccc atggattctg actggtttcg ctgatgccga aggatcattc     1800

atactagaca tccgcaaccg aaacaacgaa agcaacagat accgaacttc gctgagattc     1860

cagatcaccc tgcacaacaa ggacaaatcg attctggaga atatccagtc cacttggaag     1920

gtcggcaaga tcacaaacag cagtgacaga gccgtcatgc tgagggtcac ccgtttcgaa     1980

gatttgaaag tgattatcga ccacttcgag aaatatccgc tgattaccca gaaattgggc     2040

gattacaagt tgtttaaaca ggcattcagc gtcatggaga ataaagaaca tcttaaggag     2100

aatgggatta aggagctcgt acgaatcaaa gctaagatga attggggtct caatgacgaa     2160

ttgaaaaaag catttccaga gaacattagc aaagagcgcc cccttatcaa taagaacatt     2220

ccgaatttca aatggctggc tggattcaca gctggtgaag gctacttcgg cgtgaatcta     2280

aaaaaggtaa agggcaccgc aaaggtatac gtgggactga gattctcaat ctcacagcac     2340

atcagagaca agaacctgat gaattcattg ataacatacc taggctgtgg ttccatctgg     2400

gagaagaaca agtctgagtt cagatggctc gagttcgtcg taaccaaatt cagcgatatc     2460

aacgacaaga tcattccggt attccaggaa aatactctga ttggcgtcaa actcgaggac     2520

tttgaagatt ggtgcaaggt tgccaaattg atcaaagaga agaaacacct gaccgaatcc     2580

ggtttggatg agattaagaa aatcaagctg aacatgaaca aaggtcgtgg tggaggcggt     2640

agcggaggcg gagggtcggc tagcgccgaa ttaccgacat gcgaaacctt cgtgttcctc     2700

gacctggaag ccactggcct gcccaatgct catcccgaga ttgccgaaat ctccctgttc     2760

gccattcatc ggttctccct ggaacaccct gagagagacg agagcggaac acttcaactg     2820

ccaagggtgc tggacaagct gaccctgtgc atgtgtcccg agcagaactt cactcccaag     2880

gcatccgaaa tcactggtct gagtaatcag aacctggctg ataatcacaa ggctggattc     2940

aatggtgccg tgattcgggc tttgcgggag ttcctgaaga ggcagaaaag tccaatctgc     3000

ttggtcgccc acaacggttt tgactacgac ttcccactgc tcaggaccga acttcaacgc     3060

ctgggagcag accttcctgg aggaaccgtg tgcctggaca ccctacccgc tctgcgtgga     3120

ctggacaagg tccaccatca cagcactagg gcgtctcatg gaaagaagtc ttacagcctc     3180

ggtaatctct acaggcggta tttcgatgac gagcctcaag cagcccattc agccgaaggc     3240

gatgtctacg ccttggttat ggtcttcctg catcgggcac aagaactgct ccaatgcgta     3300

cagacggagg ctcagaattg ggatgagatt cagccgatgt ataccccacg taagcgtaga     3360

ctgtccggcg gtagcctgga tgcctga                                         3387


<210>  21
<211>  3387
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-Trex2opossum RNA 
       construct

<400>  21
augggauccu gcaggccacc uaagaagaaa cgcaaagucg uggaucuacg cacgcucggc       60

uacagucagc agcagcaaga gaagaucaaa ccgaaggugc guucgacagu ggcgcagcac      120

cacgaggcac uggugggcca uggguuuaca cacgcgcaca ucguugcgcu cagccaacac      180

ccggcagcgu uagggaccgu cgcugucacg uaucagcaca uaaucacggc guugccagag      240

gcgacacacg aagacaucgu uggcgucggc aaacaguggu ccggcgcacg cgcccuggag      300

gccuugcuca cggaugcggg ggaguugaga gguccgccgu uacaguugga cacaggccaa      360

cuugugaaga uugcaaaacg uggcggcgug accgcaaugg aggcagugca ugcaucgcgc      420

aaugcacuga cgggugcccc ccugaaccug accccggacc aagugguggc uaucgccagc      480

aacauuggcg gcaagcaagc gcucgaaacg gugcagcggc uguugccggu gcugugccag      540

gaccauggcc ugaccccgga ccaaguggug gcuaucgcca gcaacauugg cggcaagcaa      600

gcgcucgaaa cggugcagcg gcuguugccg gugcugugcc aggaccaugg ccugaccccg      660

gaccaagugg uggcuaucgc cagcaacauu ggcggcaagc aagcgcucga aacggugcag      720

cggcuguugc cggugcugug ccaggaccau ggccugaccc cggaccaagu gguggcuauc      780

gccagcaacg guggcggcaa gcaagcgcuc gaaacggugc agcggcuguu gccggugcug      840

ugccaggacc auggccugac uccggaccaa gugguggcua ucgccagcca cgauggcggc      900

aagcaagcgc ucgaaacggu gcagcggcug uugccggugc ugugccagga ccauggccug      960

acuccggacc aagugguggc uaucgccagc cacgauggcg gcaagcaagc gcucgaaacg     1020

gugcagcggc uguugccggu gcugugccag gaccauggcc ugaccccgga ccaaguggug     1080

gcuaucgcca gcaacauugg cggcaagcaa gcgcucgaaa cggugcagcg gcuguugccg     1140

gugcugugcc aggaccaugg ccugaccccg gaccaagugg uggcuaucgc cagcaacaau     1200

ggcggcaagc aagcgcucga aacggugcag cggcuguugc cggugcugug ccaggaccau     1260

ggccugaccc cggaccaagu gguggcuauc gccagcaacg guggcggcaa gcaagcgcuc     1320

gaaacggugc agcggcuguu gccggugcug ugccaggacc auggccugac cccggaccaa     1380

gugguggcua ucgccagcaa caauggcggc aagcaagcgc ucgaaacggu gcagcggcug     1440

uugccggugc ugugccagga ccauggccug accccggacc aagugguggc uaucgccagc     1500

aacauuggcg gcaagcaagc gcucgaaagc auuguggccc agcugagccg gccugauccg     1560

gcguuggccg cguugaccaa cgaccaccuc gucgccuugg ccugccucgg cggacguccu     1620

gccauggaug cagugaaaaa gggauugccg cacgcgccgg aauugaucag aagagucaau     1680

cgccguauug gcgaacgcac gucccaucgc guugcgauau cuagaguggg aggaagcucu     1740

cgcagagagu ccaucaaccc auggauucug acugguuucg cugaugccga aggaucauuc     1800

auacuagaca uccgcaaccg aaacaacgaa agcaacagau accgaacuuc gcugagauuc     1860

cagaucaccc ugcacaacaa ggacaaaucg auucuggaga auauccaguc cacuuggaag     1920

gucggcaaga ucacaaacag cagugacaga gccgucaugc ugagggucac ccguuucgaa     1980

gauuugaaag ugauuaucga ccacuucgag aaauauccgc ugauuaccca gaaauugggc     2040

gauuacaagu uguuuaaaca ggcauucagc gucauggaga auaaagaaca ucuuaaggag     2100

aaugggauua aggagcucgu acgaaucaaa gcuaagauga auuggggucu caaugacgaa     2160

uugaaaaaag cauuuccaga gaacauuagc aaagagcgcc cccuuaucaa uaagaacauu     2220

ccgaauuuca aauggcuggc uggauucaca gcuggugaag gcuacuucgg cgugaaucua     2280

aaaaagguaa agggcaccgc aaagguauac gugggacuga gauucucaau cucacagcac     2340

aucagagaca agaaccugau gaauucauug auaacauacc uaggcugugg uuccaucugg     2400

gagaagaaca agucugaguu cagauggcuc gaguucgucg uaaccaaauu cagcgauauc     2460

aacgacaaga ucauuccggu auuccaggaa aauacucuga uuggcgucaa acucgaggac     2520

uuugaagauu ggugcaaggu ugccaaauug aucaaagaga agaaacaccu gaccgaaucc     2580

gguuuggaug agauuaagaa aaucaagcug aacaugaaca aaggucgugg uggaggcggu     2640

agcggaggcg gagggucggc uagcgccgaa uuaccgacau gcgaaaccuu cguguuccuc     2700

gaccuggaag ccacuggccu gcccaaugcu caucccgaga uugccgaaau cucccuguuc     2760

gccauucauc gguucucccu ggaacacccu gagagagacg agagcggaac acuucaacug     2820

ccaagggugc uggacaagcu gacccugugc augugucccg agcagaacuu cacucccaag     2880

gcauccgaaa ucacuggucu gaguaaucag aaccuggcug auaaucacaa ggcuggauuc     2940

aauggugccg ugauucgggc uuugcgggag uuccugaaga ggcagaaaag uccaaucugc     3000

uuggucgccc acaacgguuu ugacuacgac uucccacugc ucaggaccga acuucaacgc     3060

cugggagcag accuuccugg aggaaccgug ugccuggaca cccuacccgc ucugcgugga     3120

cuggacaagg uccaccauca cagcacuagg gcgucucaug gaaagaaguc uuacagccuc     3180

gguaaucucu acaggcggua uuucgaugac gagccucaag cagcccauuc agccgaaggc     3240

gaugucuacg ccuugguuau ggucuuccug caucgggcac aagaacugcu ccaaugcgua     3300

cagacggagg cucagaauug ggaugagauu cagccgaugu auaccccacg uaagcguaga     3360

cuguccggcg guagccugga ugccuga                                         3387


<210>  22
<211>  1128
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-Trex2opossum protein 
       construct

<400>  22

Met Gly Ser Cys Arg Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser Ile Val 
            500                 505                 510         


Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp 
        515                 520                 525             


His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala 
    530                 535                 540                 


Val Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn 
545                 550                 555                 560 


Arg Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val 
                565                 570                 575     


Gly Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly 
            580                 585                 590         


Phe Ala Asp Ala Glu Gly Ser Phe Ile Leu Asp Ile Arg Asn Arg Asn 
        595                 600                 605             


Asn Glu Ser Asn Arg Tyr Arg Thr Ser Leu Arg Phe Gln Ile Thr Leu 
    610                 615                 620                 


His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys 
625                 630                 635                 640 


Val Gly Lys Ile Thr Asn Ser Ser Asp Arg Ala Val Met Leu Arg Val 
                645                 650                 655     


Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr 
            660                 665                 670         


Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala 
        675                 680                 685             


Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys 
    690                 695                 700                 


Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp Glu 
705                 710                 715                 720 


Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile 
                725                 730                 735     


Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ala Gly 
            740                 745                 750         


Glu Gly Tyr Phe Gly Val Asn Leu Lys Lys Val Lys Gly Thr Ala Lys 
        755                 760                 765             


Val Tyr Val Gly Leu Arg Phe Ser Ile Ser Gln His Ile Arg Asp Lys 
    770                 775                 780                 


Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Ser Ile Trp 
785                 790                 795                 800 


Glu Lys Asn Lys Ser Glu Phe Arg Trp Leu Glu Phe Val Val Thr Lys 
                805                 810                 815     


Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr 
            820                 825                 830         


Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala 
        835                 840                 845             


Lys Leu Ile Lys Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu 
    850                 855                 860                 


Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Gly Gly Gly Gly 
865                 870                 875                 880 


Ser Gly Gly Gly Gly Ser Ala Ser Ala Glu Leu Pro Thr Cys Glu Thr 
                885                 890                 895     


Phe Val Phe Leu Asp Leu Glu Ala Thr Gly Leu Pro Asn Ala His Pro 
            900                 905                 910         


Glu Ile Ala Glu Ile Ser Leu Phe Ala Ile His Arg Phe Ser Leu Glu 
        915                 920                 925             


His Pro Glu Arg Asp Glu Ser Gly Thr Leu Gln Leu Pro Arg Val Leu 
    930                 935                 940                 


Asp Lys Leu Thr Leu Cys Met Cys Pro Glu Gln Asn Phe Thr Pro Lys 
945                 950                 955                 960 


Ala Ser Glu Ile Thr Gly Leu Ser Asn Gln Asn Leu Ala Asp Asn His 
                965                 970                 975     


Lys Ala Gly Phe Asn Gly Ala Val Ile Arg Ala Leu Arg Glu Phe Leu 
            980                 985                 990         


Lys Arg Gln Lys Ser Pro Ile Cys  Leu Val Ala His Asn  Gly Phe Asp 
        995                 1000                 1005             


Tyr Asp  Phe Pro Leu Leu Arg  Thr Glu Leu Gln Arg  Leu Gly Ala 
    1010                 1015                 1020             


Asp Leu  Pro Gly Gly Thr Val  Cys Leu Asp Thr Leu  Pro Ala Leu 
    1025                 1030                 1035             


Arg Gly  Leu Asp Lys Val His  His His Ser Thr Arg  Ala Ser His 
    1040                 1045                 1050             


Gly Lys  Lys Ser Tyr Ser Leu  Gly Asn Leu Tyr Arg  Arg Tyr Phe 
    1055                 1060                 1065             


Asp Asp  Glu Pro Gln Ala Ala  His Ser Ala Glu Gly  Asp Val Tyr 
    1070                 1075                 1080             


Ala Leu  Val Met Val Phe Leu  His Arg Ala Gln Glu  Leu Leu Gln 
    1085                 1090                 1095             


Cys Val  Gln Thr Glu Ala Gln  Asn Trp Asp Glu Ile  Gln Pro Met 
    1100                 1105                 1110             


Tyr Thr  Pro Arg Lys Arg Arg  Leu Ser Gly Gly Ser  Leu Asp Ala 
    1115                 1120                 1125             


<210>  23
<211>  3372
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-Trex2sheep DNA 
       construct

<400>  23
atgggatcct gcaggccacc taagaagaaa cgcaaagtcg tggatctacg cacgctcggc       60

tacagtcagc agcagcaaga gaagatcaaa ccgaaggtgc gttcgacagt ggcgcagcac      120

cacgaggcac tggtgggcca tgggtttaca cacgcgcaca tcgttgcgct cagccaacac      180

ccggcagcgt tagggaccgt cgctgtcacg tatcagcaca taatcacggc gttgccagag      240

gcgacacacg aagacatcgt tggcgtcggc aaacagtggt ccggcgcacg cgccctggag      300

gccttgctca cggatgcggg ggagttgaga ggtccgccgt tacagttgga cacaggccaa      360

cttgtgaaga ttgcaaaacg tggcggcgtg accgcaatgg aggcagtgca tgcatcgcgc      420

aatgcactga cgggtgcccc cctgaacctg accccggacc aagtggtggc tatcgccagc      480

aacattggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag      540

gaccatggcc tgaccccgga ccaagtggtg gctatcgcca gcaacattgg cggcaagcaa      600

gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg      660

gaccaagtgg tggctatcgc cagcaacatt ggcggcaagc aagcgctcga aacggtgcag      720

cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc      780

gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg      840

tgccaggacc atggcctgac tccggaccaa gtggtggcta tcgccagcca cgatggcggc      900

aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg      960

actccggacc aagtggtggc tatcgccagc cacgatggcg gcaagcaagc gctcgaaacg     1020

gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg     1080

gctatcgcca gcaacattgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg     1140

gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc cagcaacaat     1200

ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat     1260

ggcctgaccc cggaccaagt ggtggctatc gccagcaacg gtggcggcaa gcaagcgctc     1320

gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa     1380

gtggtggcta tcgccagcaa caatggcggc aagcaagcgc tcgaaacggt gcagcggctg     1440

ttgccggtgc tgtgccagga ccatggcctg accccggacc aagtggtggc tatcgccagc     1500

aacattggcg gcaagcaagc gctcgaaagc attgtggccc agctgagccg gcctgatccg     1560

gcgttggccg cgttgaccaa cgaccacctc gtcgccttgg cctgcctcgg cggacgtcct     1620

gccatggatg cagtgaaaaa gggattgccg cacgcgccgg aattgatcag aagagtcaat     1680

cgccgtattg gcgaacgcac gtcccatcgc gttgcgatat ctagagtggg aggaagctct     1740

cgcagagagt ccatcaaccc atggattctg actggtttcg ctgatgccga aggatcattc     1800

atactagaca tccgcaaccg aaacaacgaa agcaacagat accgaacttc gctgagattc     1860

cagatcaccc tgcacaacaa ggacaaatcg attctggaga atatccagtc cacttggaag     1920

gtcggcaaga tcacaaacag cagtgacaga gccgtcatgc tgagggtcac ccgtttcgaa     1980

gatttgaaag tgattatcga ccacttcgag aaatatccgc tgattaccca gaaattgggc     2040

gattacaagt tgtttaaaca ggcattcagc gtcatggaga ataaagaaca tcttaaggag     2100

aatgggatta aggagctcgt acgaatcaaa gctaagatga attggggtct caatgacgaa     2160

ttgaaaaaag catttccaga gaacattagc aaagagcgcc cccttatcaa taagaacatt     2220

ccgaatttca aatggctggc tggattcaca gctggtgaag gctacttcgg cgtgaatcta     2280

aaaaaggtaa agggcaccgc aaaggtatac gtgggactga gattctcaat ctcacagcac     2340

atcagagaca agaacctgat gaattcattg ataacatacc taggctgtgg ttccatctgg     2400

gagaagaaca agtctgagtt cagatggctc gagttcgtcg taaccaaatt cagcgatatc     2460

aacgacaaga tcattccggt attccaggaa aatactctga ttggcgtcaa actcgaggac     2520

tttgaagatt ggtgcaaggt tgccaaattg atcaaagaga agaaacacct gaccgaatcc     2580

ggtttggatg agattaagaa aatcaagctg aacatgaaca aaggtcgtgg tggaggcggt     2640

agcggaggcg gagggtcggc tagctccaaa ggaccgagag ccaagacctt cgtgttcctc     2700

gacctggaag ccactggcct gcccagtgtt gatcccgaga ttgccgagct gtccctgttc     2760

gccgttcatc ggtcctccct ggaagaccct gagtgcgacg agagcggatc accgctgccg     2820

ccaagggtga tggacaagct gaccctgtgc atgtctccca aatacccctt cactgccaag     2880

gcatccgaaa tcactggtct gagtagggaa gggctgggtc gttgtggaaa ggctggattc     2940

gatgggaccg tggttcggac tttgcaggca ttcctgtcca ggcaggaagg tccagtctgc     3000

ttggtcgccc acaacggttt taactacgac ttcccactgc tctgtaccga actgcgtcgc     3060

ctgggagcac accttcctca agatacaatt tgcctggata ccctagtcgc tcttcaagat     3120

ctggacagag cccacggaca cggcactagg gcgcaaggag gcaagtctta cagcctcggt     3180

tcactgttct gttgctattt ccaggcagag ccttccgcag cccattcagc cgaaggcgat     3240

gtccacacct tgcttatggt cttcctgcat cgtgcagcag aactgctcgc ctgggcagat     3300

gagcaggctc tttcatgggc tcacgttgag ccgatgtacg tgccacctga tgacccaagg     3360

ctggaagact ga                                                         3372


<210>  24
<211>  3372
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-Trex2sheep RNA 
       construct

<400>  24
augggauccu gcaggccacc uaagaagaaa cgcaaagucg uggaucuacg cacgcucggc       60

uacagucagc agcagcaaga gaagaucaaa ccgaaggugc guucgacagu ggcgcagcac      120

cacgaggcac uggugggcca uggguuuaca cacgcgcaca ucguugcgcu cagccaacac      180

ccggcagcgu uagggaccgu cgcugucacg uaucagcaca uaaucacggc guugccagag      240

gcgacacacg aagacaucgu uggcgucggc aaacaguggu ccggcgcacg cgcccuggag      300

gccuugcuca cggaugcggg ggaguugaga gguccgccgu uacaguugga cacaggccaa      360

cuugugaaga uugcaaaacg uggcggcgug accgcaaugg aggcagugca ugcaucgcgc      420

aaugcacuga cgggugcccc ccugaaccug accccggacc aagugguggc uaucgccagc      480

aacauuggcg gcaagcaagc gcucgaaacg gugcagcggc uguugccggu gcugugccag      540

gaccauggcc ugaccccgga ccaaguggug gcuaucgcca gcaacauugg cggcaagcaa      600

gcgcucgaaa cggugcagcg gcuguugccg gugcugugcc aggaccaugg ccugaccccg      660

gaccaagugg uggcuaucgc cagcaacauu ggcggcaagc aagcgcucga aacggugcag      720

cggcuguugc cggugcugug ccaggaccau ggccugaccc cggaccaagu gguggcuauc      780

gccagcaacg guggcggcaa gcaagcgcuc gaaacggugc agcggcuguu gccggugcug      840

ugccaggacc auggccugac uccggaccaa gugguggcua ucgccagcca cgauggcggc      900

aagcaagcgc ucgaaacggu gcagcggcug uugccggugc ugugccagga ccauggccug      960

acuccggacc aagugguggc uaucgccagc cacgauggcg gcaagcaagc gcucgaaacg     1020

gugcagcggc uguugccggu gcugugccag gaccauggcc ugaccccgga ccaaguggug     1080

gcuaucgcca gcaacauugg cggcaagcaa gcgcucgaaa cggugcagcg gcuguugccg     1140

gugcugugcc aggaccaugg ccugaccccg gaccaagugg uggcuaucgc cagcaacaau     1200

ggcggcaagc aagcgcucga aacggugcag cggcuguugc cggugcugug ccaggaccau     1260

ggccugaccc cggaccaagu gguggcuauc gccagcaacg guggcggcaa gcaagcgcuc     1320

gaaacggugc agcggcuguu gccggugcug ugccaggacc auggccugac cccggaccaa     1380

gugguggcua ucgccagcaa caauggcggc aagcaagcgc ucgaaacggu gcagcggcug     1440

uugccggugc ugugccagga ccauggccug accccggacc aagugguggc uaucgccagc     1500

aacauuggcg gcaagcaagc gcucgaaagc auuguggccc agcugagccg gccugauccg     1560

gcguuggccg cguugaccaa cgaccaccuc gucgccuugg ccugccucgg cggacguccu     1620

gccauggaug cagugaaaaa gggauugccg cacgcgccgg aauugaucag aagagucaau     1680

cgccguauug gcgaacgcac gucccaucgc guugcgauau cuagaguggg aggaagcucu     1740

cgcagagagu ccaucaaccc auggauucug acugguuucg cugaugccga aggaucauuc     1800

auacuagaca uccgcaaccg aaacaacgaa agcaacagau accgaacuuc gcugagauuc     1860

cagaucaccc ugcacaacaa ggacaaaucg auucuggaga auauccaguc cacuuggaag     1920

gucggcaaga ucacaaacag cagugacaga gccgucaugc ugagggucac ccguuucgaa     1980

gauuugaaag ugauuaucga ccacuucgag aaauauccgc ugauuaccca gaaauugggc     2040

gauuacaagu uguuuaaaca ggcauucagc gucauggaga auaaagaaca ucuuaaggag     2100

aaugggauua aggagcucgu acgaaucaaa gcuaagauga auuggggucu caaugacgaa     2160

uugaaaaaag cauuuccaga gaacauuagc aaagagcgcc cccuuaucaa uaagaacauu     2220

ccgaauuuca aauggcuggc uggauucaca gcuggugaag gcuacuucgg cgugaaucua     2280

aaaaagguaa agggcaccgc aaagguauac gugggacuga gauucucaau cucacagcac     2340

aucagagaca agaaccugau gaauucauug auaacauacc uaggcugugg uuccaucugg     2400

gagaagaaca agucugaguu cagauggcuc gaguucgucg uaaccaaauu cagcgauauc     2460

aacgacaaga ucauuccggu auuccaggaa aauacucuga uuggcgucaa acucgaggac     2520

uuugaagauu ggugcaaggu ugccaaauug aucaaagaga agaaacaccu gaccgaaucc     2580

gguuuggaug agauuaagaa aaucaagcug aacaugaaca aaggucgugg uggaggcggu     2640

agcggaggcg gagggucggc uagcuccaaa ggaccgagag ccaagaccuu cguguuccuc     2700

gaccuggaag ccacuggccu gcccaguguu gaucccgaga uugccgagcu gucccuguuc     2760

gccguucauc gguccucccu ggaagacccu gagugcgacg agagcggauc accgcugccg     2820

ccaaggguga uggacaagcu gacccugugc augucuccca aauaccccuu cacugccaag     2880

gcauccgaaa ucacuggucu gaguagggaa gggcuggguc guuguggaaa ggcuggauuc     2940

gaugggaccg ugguucggac uuugcaggca uuccugucca ggcaggaagg uccagucugc     3000

uuggucgccc acaacgguuu uaacuacgac uucccacugc ucuguaccga acugcgucgc     3060

cugggagcac accuuccuca agauacaauu ugccuggaua cccuagucgc ucuucaagau     3120

cuggacagag cccacggaca cggcacuagg gcgcaaggag gcaagucuua cagccucggu     3180

ucacuguucu guugcuauuu ccaggcagag ccuuccgcag cccauucagc cgaaggcgau     3240

guccacaccu ugcuuauggu cuuccugcau cgugcagcag aacugcucgc cugggcagau     3300

gagcaggcuc uuucaugggc ucacguugag ccgauguacg ugccaccuga ugacccaagg     3360

cuggaagacu ga                                                         3372


<210>  25
<211>  1123
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-Trex2sheep protein 
       construct

<400>  25

Met Gly Ser Cys Arg Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser Ile Val 
            500                 505                 510         


Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp 
        515                 520                 525             


His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala 
    530                 535                 540                 


Val Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn 
545                 550                 555                 560 


Arg Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val 
                565                 570                 575     


Gly Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly 
            580                 585                 590         


Phe Ala Asp Ala Glu Gly Ser Phe Ile Leu Asp Ile Arg Asn Arg Asn 
        595                 600                 605             


Asn Glu Ser Asn Arg Tyr Arg Thr Ser Leu Arg Phe Gln Ile Thr Leu 
    610                 615                 620                 


His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys 
625                 630                 635                 640 


Val Gly Lys Ile Thr Asn Ser Ser Asp Arg Ala Val Met Leu Arg Val 
                645                 650                 655     


Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr 
            660                 665                 670         


Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala 
        675                 680                 685             


Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys 
    690                 695                 700                 


Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp Glu 
705                 710                 715                 720 


Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile 
                725                 730                 735     


Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ala Gly 
            740                 745                 750         


Glu Gly Tyr Phe Gly Val Asn Leu Lys Lys Val Lys Gly Thr Ala Lys 
        755                 760                 765             


Val Tyr Val Gly Leu Arg Phe Ser Ile Ser Gln His Ile Arg Asp Lys 
    770                 775                 780                 


Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Ser Ile Trp 
785                 790                 795                 800 


Glu Lys Asn Lys Ser Glu Phe Arg Trp Leu Glu Phe Val Val Thr Lys 
                805                 810                 815     


Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr 
            820                 825                 830         


Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala 
        835                 840                 845             


Lys Leu Ile Lys Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu 
    850                 855                 860                 


Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Gly Gly Gly Gly 
865                 870                 875                 880 


Ser Gly Gly Gly Gly Ser Ala Ser Ser Lys Gly Pro Arg Ala Lys Thr 
                885                 890                 895     


Phe Val Phe Leu Asp Leu Glu Ala Thr Gly Leu Pro Ser Val Asp Pro 
            900                 905                 910         


Glu Ile Ala Glu Leu Ser Leu Phe Ala Val His Arg Ser Ser Leu Glu 
        915                 920                 925             


Asp Pro Glu Cys Asp Glu Ser Gly Ser Pro Leu Pro Pro Arg Val Met 
    930                 935                 940                 


Asp Lys Leu Thr Leu Cys Met Ser Pro Lys Tyr Pro Phe Thr Ala Lys 
945                 950                 955                 960 


Ala Ser Glu Ile Thr Gly Leu Ser Arg Glu Gly Leu Gly Arg Cys Gly 
                965                 970                 975     


Lys Ala Gly Phe Asp Gly Thr Val Val Arg Thr Leu Gln Ala Phe Leu 
            980                 985                 990         


Ser Arg Gln Glu Gly Pro Val Cys  Leu Val Ala His Asn  Gly Phe Asn 
        995                 1000                 1005             


Tyr Asp  Phe Pro Leu Leu Cys  Thr Glu Leu Arg Arg  Leu Gly Ala 
    1010                 1015                 1020             


His Leu  Pro Gln Asp Thr Ile  Cys Leu Asp Thr Leu  Val Ala Leu 
    1025                 1030                 1035             


Gln Asp  Leu Asp Arg Ala His  Gly His Gly Thr Arg  Ala Gln Gly 
    1040                 1045                 1050             


Gly Lys  Ser Tyr Ser Leu Gly  Ser Leu Phe Cys Cys  Tyr Phe Gln 
    1055                 1060                 1065             


Ala Glu  Pro Ser Ala Ala His  Ser Ala Glu Gly Asp  Val His Thr 
    1070                 1075                 1080             


Leu Leu  Met Val Phe Leu His  Arg Ala Ala Glu Leu  Leu Ala Trp 
    1085                 1090                 1095             


Ala Asp  Glu Gln Ala Leu Ser  Trp Ala His Val Glu  Pro Met Tyr 
    1100                 1105                 1110             


Val Pro  Pro Asp Asp Pro Arg  Leu Glu Asp 
    1115                 1120             


<210>  26
<211>  3372
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-Trex2armadillo DNA 
       construct

<400>  26
atgggatcct gcaggccacc taagaagaaa cgcaaagtcg tggatctacg cacgctcggc       60

tacagtcagc agcagcaaga gaagatcaaa ccgaaggtgc gttcgacagt ggcgcagcac      120

cacgaggcac tggtgggcca tgggtttaca cacgcgcaca tcgttgcgct cagccaacac      180

ccggcagcgt tagggaccgt cgctgtcacg tatcagcaca taatcacggc gttgccagag      240

gcgacacacg aagacatcgt tggcgtcggc aaacagtggt ccggcgcacg cgccctggag      300

gccttgctca cggatgcggg ggagttgaga ggtccgccgt tacagttgga cacaggccaa      360

cttgtgaaga ttgcaaaacg tggcggcgtg accgcaatgg aggcagtgca tgcatcgcgc      420

aatgcactga cgggtgcccc cctgaacctg accccggacc aagtggtggc tatcgccagc      480

aacattggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag      540

gaccatggcc tgaccccgga ccaagtggtg gctatcgcca gcaacattgg cggcaagcaa      600

gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg      660

gaccaagtgg tggctatcgc cagcaacatt ggcggcaagc aagcgctcga aacggtgcag      720

cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc      780

gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg      840

tgccaggacc atggcctgac tccggaccaa gtggtggcta tcgccagcca cgatggcggc      900

aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg      960

actccggacc aagtggtggc tatcgccagc cacgatggcg gcaagcaagc gctcgaaacg     1020

gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg     1080

gctatcgcca gcaacattgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg     1140

gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc cagcaacaat     1200

ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat     1260

ggcctgaccc cggaccaagt ggtggctatc gccagcaacg gtggcggcaa gcaagcgctc     1320

gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa     1380

gtggtggcta tcgccagcaa caatggcggc aagcaagcgc tcgaaacggt gcagcggctg     1440

ttgccggtgc tgtgccagga ccatggcctg accccggacc aagtggtggc tatcgccagc     1500

aacattggcg gcaagcaagc gctcgaaagc attgtggccc agctgagccg gcctgatccg     1560

gcgttggccg cgttgaccaa cgaccacctc gtcgccttgg cctgcctcgg cggacgtcct     1620

gccatggatg cagtgaaaaa gggattgccg cacgcgccgg aattgatcag aagagtcaat     1680

cgccgtattg gcgaacgcac gtcccatcgc gttgcgatat ctagagtggg aggaagctct     1740

cgcagagagt ccatcaaccc atggattctg actggtttcg ctgatgccga aggatcattc     1800

atactagaca tccgcaaccg aaacaacgaa agcaacagat accgaacttc gctgagattc     1860

cagatcaccc tgcacaacaa ggacaaatcg attctggaga atatccagtc cacttggaag     1920

gtcggcaaga tcacaaacag cagtgacaga gccgtcatgc tgagggtcac ccgtttcgaa     1980

gatttgaaag tgattatcga ccacttcgag aaatatccgc tgattaccca gaaattgggc     2040

gattacaagt tgtttaaaca ggcattcagc gtcatggaga ataaagaaca tcttaaggag     2100

aatgggatta aggagctcgt acgaatcaaa gctaagatga attggggtct caatgacgaa     2160

ttgaaaaaag catttccaga gaacattagc aaagagcgcc cccttatcaa taagaacatt     2220

ccgaatttca aatggctggc tggattcaca gctggtgaag gctacttcgg cgtgaatcta     2280

aaaaaggtaa agggcaccgc aaaggtatac gtgggactga gattctcaat ctcacagcac     2340

atcagagaca agaacctgat gaattcattg ataacatacc taggctgtgg ttccatctgg     2400

gagaagaaca agtctgagtt cagatggctc gagttcgtcg taaccaaatt cagcgatatc     2460

aacgacaaga tcattccggt attccaggaa aatactctga ttggcgtcaa actcgaggac     2520

tttgaagatt ggtgcaaggt tgccaaattg atcaaagaga agaaacacct gaccgaatcc     2580

ggtttggatg agattaagaa aatcaagctg aacatgaaca aaggtcgtgg tggaggcggt     2640

agcggaggcg gagggtcggc tagctcccaa gcaccgcaag ccgaaacctt cgtgttcctc     2700

gacctggaag ccactggcct gcccaatgtt gatcccgaga ttgccgaaat ctccctgttc     2760

gccgttcatc ggtcctccct ggaacaccct gagagagacg aggccggagt acctgtgctg     2820

ccaagggtgc tggacaagct gaccctgtgc atgtctccca ctcgtccctt cactgccaag     2880

gcatccgaaa tcactggtct gagttcggaa gggctggctc gttgtggaaa ggctggattc     2940

gatgatactg cggctcagac tttgcgggca ttcctgggca ggcaggcagg tccactctgc     3000

ttggtcgccc acaacggttt tgactacgac ttcccactgc tcaggaccga acttcaacgc     3060

ctgggagcac gccttccagc gcataccgtg tgcctggaca ccctacccgc tctgcgtgga     3120

ctggacagag cccactcaca cgctactagg gcgcaaggac gcaagggtta cagcctcggt     3180

tcactgttca ggcggtattt ccaggcagag ccttccgcag cccattcagc cgaaggcgat     3240

gtccgcacct tgcttctggt cttcctgcat cgtgcagcag aactgctcgc ctgggcagat     3300

gggcgggctc gttcatgggc tcacattgcg ccgatgtacg cgccaccgga ggcccctagc     3360

ccggaagcct ga                                                         3372


<210>  27
<211>  3372
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-Trex2armadillo RNA 
       construct

<400>  27
augggauccu gcaggccacc uaagaagaaa cgcaaagucg uggaucuacg cacgcucggc       60

uacagucagc agcagcaaga gaagaucaaa ccgaaggugc guucgacagu ggcgcagcac      120

cacgaggcac uggugggcca uggguuuaca cacgcgcaca ucguugcgcu cagccaacac      180

ccggcagcgu uagggaccgu cgcugucacg uaucagcaca uaaucacggc guugccagag      240

gcgacacacg aagacaucgu uggcgucggc aaacaguggu ccggcgcacg cgcccuggag      300

gccuugcuca cggaugcggg ggaguugaga gguccgccgu uacaguugga cacaggccaa      360

cuugugaaga uugcaaaacg uggcggcgug accgcaaugg aggcagugca ugcaucgcgc      420

aaugcacuga cgggugcccc ccugaaccug accccggacc aagugguggc uaucgccagc      480

aacauuggcg gcaagcaagc gcucgaaacg gugcagcggc uguugccggu gcugugccag      540

gaccauggcc ugaccccgga ccaaguggug gcuaucgcca gcaacauugg cggcaagcaa      600

gcgcucgaaa cggugcagcg gcuguugccg gugcugugcc aggaccaugg ccugaccccg      660

gaccaagugg uggcuaucgc cagcaacauu ggcggcaagc aagcgcucga aacggugcag      720

cggcuguugc cggugcugug ccaggaccau ggccugaccc cggaccaagu gguggcuauc      780

gccagcaacg guggcggcaa gcaagcgcuc gaaacggugc agcggcuguu gccggugcug      840

ugccaggacc auggccugac uccggaccaa gugguggcua ucgccagcca cgauggcggc      900

aagcaagcgc ucgaaacggu gcagcggcug uugccggugc ugugccagga ccauggccug      960

acuccggacc aagugguggc uaucgccagc cacgauggcg gcaagcaagc gcucgaaacg     1020

gugcagcggc uguugccggu gcugugccag gaccauggcc ugaccccgga ccaaguggug     1080

gcuaucgcca gcaacauugg cggcaagcaa gcgcucgaaa cggugcagcg gcuguugccg     1140

gugcugugcc aggaccaugg ccugaccccg gaccaagugg uggcuaucgc cagcaacaau     1200

ggcggcaagc aagcgcucga aacggugcag cggcuguugc cggugcugug ccaggaccau     1260

ggccugaccc cggaccaagu gguggcuauc gccagcaacg guggcggcaa gcaagcgcuc     1320

gaaacggugc agcggcuguu gccggugcug ugccaggacc auggccugac cccggaccaa     1380

gugguggcua ucgccagcaa caauggcggc aagcaagcgc ucgaaacggu gcagcggcug     1440

uugccggugc ugugccagga ccauggccug accccggacc aagugguggc uaucgccagc     1500

aacauuggcg gcaagcaagc gcucgaaagc auuguggccc agcugagccg gccugauccg     1560

gcguuggccg cguugaccaa cgaccaccuc gucgccuugg ccugccucgg cggacguccu     1620

gccauggaug cagugaaaaa gggauugccg cacgcgccgg aauugaucag aagagucaau     1680

cgccguauug gcgaacgcac gucccaucgc guugcgauau cuagaguggg aggaagcucu     1740

cgcagagagu ccaucaaccc auggauucug acugguuucg cugaugccga aggaucauuc     1800

auacuagaca uccgcaaccg aaacaacgaa agcaacagau accgaacuuc gcugagauuc     1860

cagaucaccc ugcacaacaa ggacaaaucg auucuggaga auauccaguc cacuuggaag     1920

gucggcaaga ucacaaacag cagugacaga gccgucaugc ugagggucac ccguuucgaa     1980

gauuugaaag ugauuaucga ccacuucgag aaauauccgc ugauuaccca gaaauugggc     2040

gauuacaagu uguuuaaaca ggcauucagc gucauggaga auaaagaaca ucuuaaggag     2100

aaugggauua aggagcucgu acgaaucaaa gcuaagauga auuggggucu caaugacgaa     2160

uugaaaaaag cauuuccaga gaacauuagc aaagagcgcc cccuuaucaa uaagaacauu     2220

ccgaauuuca aauggcuggc uggauucaca gcuggugaag gcuacuucgg cgugaaucua     2280

aaaaagguaa agggcaccgc aaagguauac gugggacuga gauucucaau cucacagcac     2340

aucagagaca agaaccugau gaauucauug auaacauacc uaggcugugg uuccaucugg     2400

gagaagaaca agucugaguu cagauggcuc gaguucgucg uaaccaaauu cagcgauauc     2460

aacgacaaga ucauuccggu auuccaggaa aauacucuga uuggcgucaa acucgaggac     2520

uuugaagauu ggugcaaggu ugccaaauug aucaaagaga agaaacaccu gaccgaaucc     2580

gguuuggaug agauuaagaa aaucaagcug aacaugaaca aaggucgugg uggaggcggu     2640

agcggaggcg gagggucggc uagcucccaa gcaccgcaag ccgaaaccuu cguguuccuc     2700

gaccuggaag ccacuggccu gcccaauguu gaucccgaga uugccgaaau cucccuguuc     2760

gccguucauc gguccucccu ggaacacccu gagagagacg aggccggagu accugugcug     2820

ccaagggugc uggacaagcu gacccugugc augucuccca cucgucccuu cacugccaag     2880

gcauccgaaa ucacuggucu gaguucggaa gggcuggcuc guuguggaaa ggcuggauuc     2940

gaugauacug cggcucagac uuugcgggca uuccugggca ggcaggcagg uccacucugc     3000

uuggucgccc acaacgguuu ugacuacgac uucccacugc ucaggaccga acuucaacgc     3060

cugggagcac gccuuccagc gcauaccgug ugccuggaca cccuacccgc ucugcgugga     3120

cuggacagag cccacucaca cgcuacuagg gcgcaaggac gcaaggguua cagccucggu     3180

ucacuguuca ggcgguauuu ccaggcagag ccuuccgcag cccauucagc cgaaggcgau     3240

guccgcaccu ugcuucuggu cuuccugcau cgugcagcag aacugcucgc cugggcagau     3300

gggcgggcuc guucaugggc ucacauugcg ccgauguacg cgccaccgga ggccccuagc     3360

ccggaagccu ga                                                         3372


<210>  28
<211>  1123
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-Trex2armadillo 
       protein construct

<400>  28

Met Gly Ser Cys Arg Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser Ile Val 
            500                 505                 510         


Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp 
        515                 520                 525             


His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala 
    530                 535                 540                 


Val Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn 
545                 550                 555                 560 


Arg Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val 
                565                 570                 575     


Gly Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly 
            580                 585                 590         


Phe Ala Asp Ala Glu Gly Ser Phe Ile Leu Asp Ile Arg Asn Arg Asn 
        595                 600                 605             


Asn Glu Ser Asn Arg Tyr Arg Thr Ser Leu Arg Phe Gln Ile Thr Leu 
    610                 615                 620                 


His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys 
625                 630                 635                 640 


Val Gly Lys Ile Thr Asn Ser Ser Asp Arg Ala Val Met Leu Arg Val 
                645                 650                 655     


Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr 
            660                 665                 670         


Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala 
        675                 680                 685             


Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys 
    690                 695                 700                 


Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp Glu 
705                 710                 715                 720 


Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile 
                725                 730                 735     


Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ala Gly 
            740                 745                 750         


Glu Gly Tyr Phe Gly Val Asn Leu Lys Lys Val Lys Gly Thr Ala Lys 
        755                 760                 765             


Val Tyr Val Gly Leu Arg Phe Ser Ile Ser Gln His Ile Arg Asp Lys 
    770                 775                 780                 


Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Ser Ile Trp 
785                 790                 795                 800 


Glu Lys Asn Lys Ser Glu Phe Arg Trp Leu Glu Phe Val Val Thr Lys 
                805                 810                 815     


Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr 
            820                 825                 830         


Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala 
        835                 840                 845             


Lys Leu Ile Lys Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu 
    850                 855                 860                 


Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Gly Gly Gly Gly 
865                 870                 875                 880 


Ser Gly Gly Gly Gly Ser Ala Ser Ser Gln Ala Pro Gln Ala Glu Thr 
                885                 890                 895     


Phe Val Phe Leu Asp Leu Glu Ala Thr Gly Leu Pro Asn Val Asp Pro 
            900                 905                 910         


Glu Ile Ala Glu Ile Ser Leu Phe Ala Val His Arg Ser Ser Leu Glu 
        915                 920                 925             


His Pro Glu Arg Asp Glu Ala Gly Val Pro Val Leu Pro Arg Val Leu 
    930                 935                 940                 


Asp Lys Leu Thr Leu Cys Met Ser Pro Thr Arg Pro Phe Thr Ala Lys 
945                 950                 955                 960 


Ala Ser Glu Ile Thr Gly Leu Ser Ser Glu Gly Leu Ala Arg Cys Gly 
                965                 970                 975     


Lys Ala Gly Phe Asp Asp Thr Ala Ala Gln Thr Leu Arg Ala Phe Leu 
            980                 985                 990         


Gly Arg Gln Ala Gly Pro Leu Cys  Leu Val Ala His Asn  Gly Phe Asp 
        995                 1000                 1005             


Tyr Asp  Phe Pro Leu Leu Arg  Thr Glu Leu Gln Arg  Leu Gly Ala 
    1010                 1015                 1020             


Arg Leu  Pro Ala His Thr Val  Cys Leu Asp Thr Leu  Pro Ala Leu 
    1025                 1030                 1035             


Arg Gly  Leu Asp Arg Ala His  Ser His Ala Thr Arg  Ala Gln Gly 
    1040                 1045                 1050             


Arg Lys  Gly Tyr Ser Leu Gly  Ser Leu Phe Arg Arg  Tyr Phe Gln 
    1055                 1060                 1065             


Ala Glu  Pro Ser Ala Ala His  Ser Ala Glu Gly Asp  Val Arg Thr 
    1070                 1075                 1080             


Leu Leu  Leu Val Phe Leu His  Arg Ala Ala Glu Leu  Leu Ala Trp 
    1085                 1090                 1095             


Ala Asp  Gly Arg Ala Arg Ser  Trp Ala His Ile Ala  Pro Met Tyr 
    1100                 1105                 1110             


Ala Pro  Pro Glu Ala Pro Ser  Pro Glu Ala 
    1115                 1120             


<210>  29
<211>  3372
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-Trex2leopard DNA 
       construct

<400>  29
atgggatcct gcaggccacc taagaagaaa cgcaaagtcg tggatctacg cacgctcggc       60

tacagtcagc agcagcaaga gaagatcaaa ccgaaggtgc gttcgacagt ggcgcagcac      120

cacgaggcac tggtgggcca tgggtttaca cacgcgcaca tcgttgcgct cagccaacac      180

ccggcagcgt tagggaccgt cgctgtcacg tatcagcaca taatcacggc gttgccagag      240

gcgacacacg aagacatcgt tggcgtcggc aaacagtggt ccggcgcacg cgccctggag      300

gccttgctca cggatgcggg ggagttgaga ggtccgccgt tacagttgga cacaggccaa      360

cttgtgaaga ttgcaaaacg tggcggcgtg accgcaatgg aggcagtgca tgcatcgcgc      420

aatgcactga cgggtgcccc cctgaacctg accccggacc aagtggtggc tatcgccagc      480

aacattggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag      540

gaccatggcc tgaccccgga ccaagtggtg gctatcgcca gcaacattgg cggcaagcaa      600

gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg      660

gaccaagtgg tggctatcgc cagcaacatt ggcggcaagc aagcgctcga aacggtgcag      720

cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc      780

gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg      840

tgccaggacc atggcctgac tccggaccaa gtggtggcta tcgccagcca cgatggcggc      900

aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg      960

actccggacc aagtggtggc tatcgccagc cacgatggcg gcaagcaagc gctcgaaacg     1020

gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg     1080

gctatcgcca gcaacattgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg     1140

gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc cagcaacaat     1200

ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat     1260

ggcctgaccc cggaccaagt ggtggctatc gccagcaacg gtggcggcaa gcaagcgctc     1320

gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa     1380

gtggtggcta tcgccagcaa caatggcggc aagcaagcgc tcgaaacggt gcagcggctg     1440

ttgccggtgc tgtgccagga ccatggcctg accccggacc aagtggtggc tatcgccagc     1500

aacattggcg gcaagcaagc gctcgaaagc attgtggccc agctgagccg gcctgatccg     1560

gcgttggccg cgttgaccaa cgaccacctc gtcgccttgg cctgcctcgg cggacgtcct     1620

gccatggatg cagtgaaaaa gggattgccg cacgcgccgg aattgatcag aagagtcaat     1680

cgccgtattg gcgaacgcac gtcccatcgc gttgcgatat ctagagtggg aggaagctct     1740

cgcagagagt ccatcaaccc atggattctg actggtttcg ctgatgccga aggatcattc     1800

atactagaca tccgcaaccg aaacaacgaa agcaacagat accgaacttc gctgagattc     1860

cagatcaccc tgcacaacaa ggacaaatcg attctggaga atatccagtc cacttggaag     1920

gtcggcaaga tcacaaacag cagtgacaga gccgtcatgc tgagggtcac ccgtttcgaa     1980

gatttgaaag tgattatcga ccacttcgag aaatatccgc tgattaccca gaaattgggc     2040

gattacaagt tgtttaaaca ggcattcagc gtcatggaga ataaagaaca tcttaaggag     2100

aatgggatta aggagctcgt acgaatcaaa gctaagatga attggggtct caatgacgaa     2160

ttgaaaaaag catttccaga gaacattagc aaagagcgcc cccttatcaa taagaacatt     2220

ccgaatttca aatggctggc tggattcaca gctggtgaag gctacttcgg cgtgaatcta     2280

aaaaaggtaa agggcaccgc aaaggtatac gtgggactga gattctcaat ctcacagcac     2340

atcagagaca agaacctgat gaattcattg ataacatacc taggctgtgg ttccatctgg     2400

gagaagaaca agtctgagtt cagatggctc gagttcgtcg taaccaaatt cagcgatatc     2460

aacgacaaga tcattccggt attccaggaa aatactctga ttggcgtcaa actcgaggac     2520

tttgaagatt ggtgcaaggt tgccaaattg atcaaagaga agaaacacct gaccgaatcc     2580

ggtttggatg agattaagaa aatcaagctg aacatgaaca aaggtcgtgg tggaggcggt     2640

agcggaggcg gagggtcggc tagctccgaa gtaccgagag ccgaaacctt cgtgttcctc     2700

gacctggaag ccactggcct gcccagtatt gatcccgagg ttgccgaaat ctccctgttc     2760

gccgttcatc ggtcctccct ggaaaaccct gacagagacg agagcggagt acctgtgctg     2820

ccaagggtgc tggacaagct gaccctgtgc atgtctcccg agcgtccctt cactgccaag     2880

gcatccgaaa tcactggtct gagttcggat tccctggctc gttgtagaaa ggctggattc     2940

gattcttccg tggttcggac tttgcaggca ttcctgtcca ggcaggaagg tccagtctgc     3000

ttggtcgccc acaacggttt tgactacgac ttcccactgc tctgtaccga actgcgtcgc     3060

ctgggagcac accttccacc ggatacaatt tgcctggaca ccctacccgc tctgcgtgga     3120

ctggacagag cccactcaca cggcactagg gcgcaaggat tcaagggtta cagcctcggt     3180

tcactgttca ggcggtattt ccatgcagag ccttccgcag cccattcagc cgaaggcgat     3240

gtccacacct tgcttatggt cttcctgcat cgtgcagcag aactgctctc ctgggcagat     3300

gagcaggctc gttcatgggc tcgcattaag ccgatgtacg agccaccgga tggccctagc     3360

gtggaagcct ga                                                         3372


<210>  30
<211>  3372
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-Trex2leopard RNA 
       construct

<400>  30
augggauccu gcaggccacc uaagaagaaa cgcaaagucg uggaucuacg cacgcucggc       60

uacagucagc agcagcaaga gaagaucaaa ccgaaggugc guucgacagu ggcgcagcac      120

cacgaggcac uggugggcca uggguuuaca cacgcgcaca ucguugcgcu cagccaacac      180

ccggcagcgu uagggaccgu cgcugucacg uaucagcaca uaaucacggc guugccagag      240

gcgacacacg aagacaucgu uggcgucggc aaacaguggu ccggcgcacg cgcccuggag      300

gccuugcuca cggaugcggg ggaguugaga gguccgccgu uacaguugga cacaggccaa      360

cuugugaaga uugcaaaacg uggcggcgug accgcaaugg aggcagugca ugcaucgcgc      420

aaugcacuga cgggugcccc ccugaaccug accccggacc aagugguggc uaucgccagc      480

aacauuggcg gcaagcaagc gcucgaaacg gugcagcggc uguugccggu gcugugccag      540

gaccauggcc ugaccccgga ccaaguggug gcuaucgcca gcaacauugg cggcaagcaa      600

gcgcucgaaa cggugcagcg gcuguugccg gugcugugcc aggaccaugg ccugaccccg      660

gaccaagugg uggcuaucgc cagcaacauu ggcggcaagc aagcgcucga aacggugcag      720

cggcuguugc cggugcugug ccaggaccau ggccugaccc cggaccaagu gguggcuauc      780

gccagcaacg guggcggcaa gcaagcgcuc gaaacggugc agcggcuguu gccggugcug      840

ugccaggacc auggccugac uccggaccaa gugguggcua ucgccagcca cgauggcggc      900

aagcaagcgc ucgaaacggu gcagcggcug uugccggugc ugugccagga ccauggccug      960

acuccggacc aagugguggc uaucgccagc cacgauggcg gcaagcaagc gcucgaaacg     1020

gugcagcggc uguugccggu gcugugccag gaccauggcc ugaccccgga ccaaguggug     1080

gcuaucgcca gcaacauugg cggcaagcaa gcgcucgaaa cggugcagcg gcuguugccg     1140

gugcugugcc aggaccaugg ccugaccccg gaccaagugg uggcuaucgc cagcaacaau     1200

ggcggcaagc aagcgcucga aacggugcag cggcuguugc cggugcugug ccaggaccau     1260

ggccugaccc cggaccaagu gguggcuauc gccagcaacg guggcggcaa gcaagcgcuc     1320

gaaacggugc agcggcuguu gccggugcug ugccaggacc auggccugac cccggaccaa     1380

gugguggcua ucgccagcaa caauggcggc aagcaagcgc ucgaaacggu gcagcggcug     1440

uugccggugc ugugccagga ccauggccug accccggacc aagugguggc uaucgccagc     1500

aacauuggcg gcaagcaagc gcucgaaagc auuguggccc agcugagccg gccugauccg     1560

gcguuggccg cguugaccaa cgaccaccuc gucgccuugg ccugccucgg cggacguccu     1620

gccauggaug cagugaaaaa gggauugccg cacgcgccgg aauugaucag aagagucaau     1680

cgccguauug gcgaacgcac gucccaucgc guugcgauau cuagaguggg aggaagcucu     1740

cgcagagagu ccaucaaccc auggauucug acugguuucg cugaugccga aggaucauuc     1800

auacuagaca uccgcaaccg aaacaacgaa agcaacagau accgaacuuc gcugagauuc     1860

cagaucaccc ugcacaacaa ggacaaaucg auucuggaga auauccaguc cacuuggaag     1920

gucggcaaga ucacaaacag cagugacaga gccgucaugc ugagggucac ccguuucgaa     1980

gauuugaaag ugauuaucga ccacuucgag aaauauccgc ugauuaccca gaaauugggc     2040

gauuacaagu uguuuaaaca ggcauucagc gucauggaga auaaagaaca ucuuaaggag     2100

aaugggauua aggagcucgu acgaaucaaa gcuaagauga auuggggucu caaugacgaa     2160

uugaaaaaag cauuuccaga gaacauuagc aaagagcgcc cccuuaucaa uaagaacauu     2220

ccgaauuuca aauggcuggc uggauucaca gcuggugaag gcuacuucgg cgugaaucua     2280

aaaaagguaa agggcaccgc aaagguauac gugggacuga gauucucaau cucacagcac     2340

aucagagaca agaaccugau gaauucauug auaacauacc uaggcugugg uuccaucugg     2400

gagaagaaca agucugaguu cagauggcuc gaguucgucg uaaccaaauu cagcgauauc     2460

aacgacaaga ucauuccggu auuccaggaa aauacucuga uuggcgucaa acucgaggac     2520

uuugaagauu ggugcaaggu ugccaaauug aucaaagaga agaaacaccu gaccgaaucc     2580

gguuuggaug agauuaagaa aaucaagcug aacaugaaca aaggucgugg uggaggcggu     2640

agcggaggcg gagggucggc uagcuccgaa guaccgagag ccgaaaccuu cguguuccuc     2700

gaccuggaag ccacuggccu gcccaguauu gaucccgagg uugccgaaau cucccuguuc     2760

gccguucauc gguccucccu ggaaaacccu gacagagacg agagcggagu accugugcug     2820

ccaagggugc uggacaagcu gacccugugc augucucccg agcgucccuu cacugccaag     2880

gcauccgaaa ucacuggucu gaguucggau ucccuggcuc guuguagaaa ggcuggauuc     2940

gauucuuccg ugguucggac uuugcaggca uuccugucca ggcaggaagg uccagucugc     3000

uuggucgccc acaacgguuu ugacuacgac uucccacugc ucuguaccga acugcgucgc     3060

cugggagcac accuuccacc ggauacaauu ugccuggaca cccuacccgc ucugcgugga     3120

cuggacagag cccacucaca cggcacuagg gcgcaaggau ucaaggguua cagccucggu     3180

ucacuguuca ggcgguauuu ccaugcagag ccuuccgcag cccauucagc cgaaggcgau     3240

guccacaccu ugcuuauggu cuuccugcau cgugcagcag aacugcucuc cugggcagau     3300

gagcaggcuc guucaugggc ucgcauuaag ccgauguacg agccaccgga uggcccuagc     3360

guggaagccu ga                                                         3372


<210>  31
<211>  1123
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-Trex2leopard protein 
       construct

<400>  31

Met Gly Ser Cys Arg Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser Ile Val 
            500                 505                 510         


Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp 
        515                 520                 525             


His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala 
    530                 535                 540                 


Val Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn 
545                 550                 555                 560 


Arg Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val 
                565                 570                 575     


Gly Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly 
            580                 585                 590         


Phe Ala Asp Ala Glu Gly Ser Phe Ile Leu Asp Ile Arg Asn Arg Asn 
        595                 600                 605             


Asn Glu Ser Asn Arg Tyr Arg Thr Ser Leu Arg Phe Gln Ile Thr Leu 
    610                 615                 620                 


His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys 
625                 630                 635                 640 


Val Gly Lys Ile Thr Asn Ser Ser Asp Arg Ala Val Met Leu Arg Val 
                645                 650                 655     


Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr 
            660                 665                 670         


Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala 
        675                 680                 685             


Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys 
    690                 695                 700                 


Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp Glu 
705                 710                 715                 720 


Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile 
                725                 730                 735     


Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ala Gly 
            740                 745                 750         


Glu Gly Tyr Phe Gly Val Asn Leu Lys Lys Val Lys Gly Thr Ala Lys 
        755                 760                 765             


Val Tyr Val Gly Leu Arg Phe Ser Ile Ser Gln His Ile Arg Asp Lys 
    770                 775                 780                 


Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Ser Ile Trp 
785                 790                 795                 800 


Glu Lys Asn Lys Ser Glu Phe Arg Trp Leu Glu Phe Val Val Thr Lys 
                805                 810                 815     


Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr 
            820                 825                 830         


Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala 
        835                 840                 845             


Lys Leu Ile Lys Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu 
    850                 855                 860                 


Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Gly Gly Gly Gly 
865                 870                 875                 880 


Ser Gly Gly Gly Gly Ser Ala Ser Ser Glu Val Pro Arg Ala Glu Thr 
                885                 890                 895     


Phe Val Phe Leu Asp Leu Glu Ala Thr Gly Leu Pro Ser Ile Asp Pro 
            900                 905                 910         


Glu Val Ala Glu Ile Ser Leu Phe Ala Val His Arg Ser Ser Leu Glu 
        915                 920                 925             


Asn Pro Asp Arg Asp Glu Ser Gly Val Pro Val Leu Pro Arg Val Leu 
    930                 935                 940                 


Asp Lys Leu Thr Leu Cys Met Ser Pro Glu Arg Pro Phe Thr Ala Lys 
945                 950                 955                 960 


Ala Ser Glu Ile Thr Gly Leu Ser Ser Asp Ser Leu Ala Arg Cys Arg 
                965                 970                 975     


Lys Ala Gly Phe Asp Ser Ser Val Val Arg Thr Leu Gln Ala Phe Leu 
            980                 985                 990         


Ser Arg Gln Glu Gly Pro Val Cys  Leu Val Ala His Asn  Gly Phe Asp 
        995                 1000                 1005             


Tyr Asp  Phe Pro Leu Leu Cys  Thr Glu Leu Arg Arg  Leu Gly Ala 
    1010                 1015                 1020             


His Leu  Pro Pro Asp Thr Ile  Cys Leu Asp Thr Leu  Pro Ala Leu 
    1025                 1030                 1035             


Arg Gly  Leu Asp Arg Ala His  Ser His Gly Thr Arg  Ala Gln Gly 
    1040                 1045                 1050             


Phe Lys  Gly Tyr Ser Leu Gly  Ser Leu Phe Arg Arg  Tyr Phe His 
    1055                 1060                 1065             


Ala Glu  Pro Ser Ala Ala His  Ser Ala Glu Gly Asp  Val His Thr 
    1070                 1075                 1080             


Leu Leu  Met Val Phe Leu His  Arg Ala Ala Glu Leu  Leu Ser Trp 
    1085                 1090                 1095             


Ala Asp  Glu Gln Ala Arg Ser  Trp Ala Arg Ile Lys  Pro Met Tyr 
    1100                 1105                 1110             


Glu Pro  Pro Asp Gly Pro Ser  Val Glu Ala 
    1115                 1120             


<210>  32
<211>  3372
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-Trex2mouse DNA 
       construct

<400>  32
atgggatcct gcaggccacc taagaagaaa cgcaaagtcg tggatctacg cacgctcggc       60

tacagtcagc agcagcaaga gaagatcaaa ccgaaggtgc gttcgacagt ggcgcagcac      120

cacgaggcac tggtgggcca tgggtttaca cacgcgcaca tcgttgcgct cagccaacac      180

ccggcagcgt tagggaccgt cgctgtcacg tatcagcaca taatcacggc gttgccagag      240

gcgacacacg aagacatcgt tggcgtcggc aaacagtggt ccggcgcacg cgccctggag      300

gccttgctca cggatgcggg ggagttgaga ggtccgccgt tacagttgga cacaggccaa      360

cttgtgaaga ttgcaaaacg tggcggcgtg accgcaatgg aggcagtgca tgcatcgcgc      420

aatgcactga cgggtgcccc cctgaacctg accccggacc aagtggtggc tatcgccagc      480

aacattggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag      540

gaccatggcc tgaccccgga ccaagtggtg gctatcgcca gcaacattgg cggcaagcaa      600

gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg      660

gaccaagtgg tggctatcgc cagcaacatt ggcggcaagc aagcgctcga aacggtgcag      720

cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc      780

gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg      840

tgccaggacc atggcctgac tccggaccaa gtggtggcta tcgccagcca cgatggcggc      900

aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg      960

actccggacc aagtggtggc tatcgccagc cacgatggcg gcaagcaagc gctcgaaacg     1020

gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg     1080

gctatcgcca gcaacattgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg     1140

gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc cagcaacaat     1200

ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat     1260

ggcctgaccc cggaccaagt ggtggctatc gccagcaacg gtggcggcaa gcaagcgctc     1320

gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa     1380

gtggtggcta tcgccagcaa caatggcggc aagcaagcgc tcgaaacggt gcagcggctg     1440

ttgccggtgc tgtgccagga ccatggcctg accccggacc aagtggtggc tatcgccagc     1500

aacattggcg gcaagcaagc gctcgaaagc attgtggccc agctgagccg gcctgatccg     1560

gcgttggccg cgttgaccaa cgaccacctc gtcgccttgg cctgcctcgg cggacgtcct     1620

gccatggatg cagtgaaaaa gggattgccg cacgcgccgg aattgatcag aagagtcaat     1680

cgccgtattg gcgaacgcac gtcccatcgc gttgcgatat ctagagtggg aggaagctct     1740

cgcagagagt ccatcaaccc atggattctg actggtttcg ctgatgccga aggatcattc     1800

atactagaca tccgcaaccg aaacaacgaa agcaacagat accgaacttc gctgagattc     1860

cagatcaccc tgcacaacaa ggacaaatcg attctggaga atatccagtc cacttggaag     1920

gtcggcaaga tcacaaacag cagtgacaga gccgtcatgc tgagggtcac ccgtttcgaa     1980

gatttgaaag tgattatcga ccacttcgag aaatatccgc tgattaccca gaaattgggc     2040

gattacaagt tgtttaaaca ggcattcagc gtcatggaga ataaagaaca tcttaaggag     2100

aatgggatta aggagctcgt acgaatcaaa gctaagatga attggggtct caatgacgaa     2160

ttgaaaaaag catttccaga gaacattagc aaagagcgcc cccttatcaa taagaacatt     2220

ccgaatttca aatggctggc tggattcaca gctggtgaag gctacttcgg cgtgaatcta     2280

aaaaaggtaa agggcaccgc aaaggtatac gtgggactga gattctcaat ctcacagcac     2340

atcagagaca agaacctgat gaattcattg ataacatacc taggctgtgg ttccatctgg     2400

gagaagaaca agtctgagtt cagatggctc gagttcgtcg taaccaaatt cagcgatatc     2460

aacgacaaga tcattccggt attccaggaa aatactctga ttggcgtcaa actcgaggac     2520

tttgaagatt ggtgcaaggt tgccaaattg atcaaagaga agaaacacct gaccgaatcc     2580

ggtttggatg agattaagaa aatcaagctg aacatgaaca aaggtcgtgg tggaggcggt     2640

agcggaggcg gagggtcggc tagctccgaa ccgccgagag ccgaaacctt cgtgttcctc     2700

gacctggagg ccactggcct gccgaacatg gacccggaaa ttgccgaaat ctccctgttc     2760

gccgtgcacc ggtcctccct ggaaaaccct gagcgggacg atagcggttc cctggtgctg     2820

cccagggtgc tggacaagct gaccctgtgc atgtgccccg agcgcccctt cactgccaag     2880

gcatccgaaa tcactggtct gagctcggaa tccctgatgc actgcggcaa ggccggattc     2940

aacggcgccg tggtccggac tttgcaggga ttcctgtcca gacaggaggg ccccatctgc     3000

ttggtcgccc acaacggttt tgactacgac ttccccctgc tctgcaccga actgcagcgc     3060

ctgggagccc acctccctca ggacaccgtg tgcctggaca ccctgcccgc actgcgcgga     3120

ctggacagag cccactcaca cggcaccagg gcgcagggac gcaagtccta cagcctcgca     3180

tcactgttcc atcggtattt ccaggccgag ccttccgccg cccattccgc cgaaggggat     3240

gtgcacacct tgcttctgat cttcctgcat cgcgctcccg aactgctggc ctgggcggac     3300

gagcaggccc ggtcctgggc ccacattgag ccgatgtacg tgccgcccga tggacctagc     3360

ctggaagcct ga                                                         3372


<210>  33
<211>  3372
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-Trex2mouse RNA 
       construct

<400>  33
augggauccu gcaggccacc uaagaagaaa cgcaaagucg uggaucuacg cacgcucggc       60

uacagucagc agcagcaaga gaagaucaaa ccgaaggugc guucgacagu ggcgcagcac      120

cacgaggcac uggugggcca uggguuuaca cacgcgcaca ucguugcgcu cagccaacac      180

ccggcagcgu uagggaccgu cgcugucacg uaucagcaca uaaucacggc guugccagag      240

gcgacacacg aagacaucgu uggcgucggc aaacaguggu ccggcgcacg cgcccuggag      300

gccuugcuca cggaugcggg ggaguugaga gguccgccgu uacaguugga cacaggccaa      360

cuugugaaga uugcaaaacg uggcggcgug accgcaaugg aggcagugca ugcaucgcgc      420

aaugcacuga cgggugcccc ccugaaccug accccggacc aagugguggc uaucgccagc      480

aacauuggcg gcaagcaagc gcucgaaacg gugcagcggc uguugccggu gcugugccag      540

gaccauggcc ugaccccgga ccaaguggug gcuaucgcca gcaacauugg cggcaagcaa      600

gcgcucgaaa cggugcagcg gcuguugccg gugcugugcc aggaccaugg ccugaccccg      660

gaccaagugg uggcuaucgc cagcaacauu ggcggcaagc aagcgcucga aacggugcag      720

cggcuguugc cggugcugug ccaggaccau ggccugaccc cggaccaagu gguggcuauc      780

gccagcaacg guggcggcaa gcaagcgcuc gaaacggugc agcggcuguu gccggugcug      840

ugccaggacc auggccugac uccggaccaa gugguggcua ucgccagcca cgauggcggc      900

aagcaagcgc ucgaaacggu gcagcggcug uugccggugc ugugccagga ccauggccug      960

acuccggacc aagugguggc uaucgccagc cacgauggcg gcaagcaagc gcucgaaacg     1020

gugcagcggc uguugccggu gcugugccag gaccauggcc ugaccccgga ccaaguggug     1080

gcuaucgcca gcaacauugg cggcaagcaa gcgcucgaaa cggugcagcg gcuguugccg     1140

gugcugugcc aggaccaugg ccugaccccg gaccaagugg uggcuaucgc cagcaacaau     1200

ggcggcaagc aagcgcucga aacggugcag cggcuguugc cggugcugug ccaggaccau     1260

ggccugaccc cggaccaagu gguggcuauc gccagcaacg guggcggcaa gcaagcgcuc     1320

gaaacggugc agcggcuguu gccggugcug ugccaggacc auggccugac cccggaccaa     1380

gugguggcua ucgccagcaa caauggcggc aagcaagcgc ucgaaacggu gcagcggcug     1440

uugccggugc ugugccagga ccauggccug accccggacc aagugguggc uaucgccagc     1500

aacauuggcg gcaagcaagc gcucgaaagc auuguggccc agcugagccg gccugauccg     1560

gcguuggccg cguugaccaa cgaccaccuc gucgccuugg ccugccucgg cggacguccu     1620

gccauggaug cagugaaaaa gggauugccg cacgcgccgg aauugaucag aagagucaau     1680

cgccguauug gcgaacgcac gucccaucgc guugcgauau cuagaguggg aggaagcucu     1740

cgcagagagu ccaucaaccc auggauucug acugguuucg cugaugccga aggaucauuc     1800

auacuagaca uccgcaaccg aaacaacgaa agcaacagau accgaacuuc gcugagauuc     1860

cagaucaccc ugcacaacaa ggacaaaucg auucuggaga auauccaguc cacuuggaag     1920

gucggcaaga ucacaaacag cagugacaga gccgucaugc ugagggucac ccguuucgaa     1980

gauuugaaag ugauuaucga ccacuucgag aaauauccgc ugauuaccca gaaauugggc     2040

gauuacaagu uguuuaaaca ggcauucagc gucauggaga auaaagaaca ucuuaaggag     2100

aaugggauua aggagcucgu acgaaucaaa gcuaagauga auuggggucu caaugacgaa     2160

uugaaaaaag cauuuccaga gaacauuagc aaagagcgcc cccuuaucaa uaagaacauu     2220

ccgaauuuca aauggcuggc uggauucaca gcuggugaag gcuacuucgg cgugaaucua     2280

aaaaagguaa agggcaccgc aaagguauac gugggacuga gauucucaau cucacagcac     2340

aucagagaca agaaccugau gaauucauug auaacauacc uaggcugugg uuccaucugg     2400

gagaagaaca agucugaguu cagauggcuc gaguucgucg uaaccaaauu cagcgauauc     2460

aacgacaaga ucauuccggu auuccaggaa aauacucuga uuggcgucaa acucgaggac     2520

uuugaagauu ggugcaaggu ugccaaauug aucaaagaga agaaacaccu gaccgaaucc     2580

gguuuggaug agauuaagaa aaucaagcug aacaugaaca aaggucgugg uggaggcggu     2640

agcggaggcg gagggucggc uagcuccgaa ccgccgagag ccgaaaccuu cguguuccuc     2700

gaccuggagg ccacuggccu gccgaacaug gacccggaaa uugccgaaau cucccuguuc     2760

gccgugcacc gguccucccu ggaaaacccu gagcgggacg auagcgguuc ccuggugcug     2820

cccagggugc uggacaagcu gacccugugc augugccccg agcgccccuu cacugccaag     2880

gcauccgaaa ucacuggucu gagcucggaa ucccugaugc acugcggcaa ggccggauuc     2940

aacggcgccg ugguccggac uuugcaggga uuccugucca gacaggaggg ccccaucugc     3000

uuggucgccc acaacgguuu ugacuacgac uucccccugc ucugcaccga acugcagcgc     3060

cugggagccc accucccuca ggacaccgug ugccuggaca cccugcccgc acugcgcgga     3120

cuggacagag cccacucaca cggcaccagg gcgcagggac gcaaguccua cagccucgca     3180

ucacuguucc aucgguauuu ccaggccgag ccuuccgccg cccauuccgc cgaaggggau     3240

gugcacaccu ugcuucugau cuuccugcau cgcgcucccg aacugcuggc cugggcggac     3300

gagcaggccc gguccugggc ccacauugag ccgauguacg ugccgcccga uggaccuagc     3360

cuggaagccu ga                                                         3372


<210>  34
<211>  1123
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-Trex2mouse protein 
       construct

<400>  34

Met Gly Ser Cys Arg Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser Ile Val 
            500                 505                 510         


Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp 
        515                 520                 525             


His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala 
    530                 535                 540                 


Val Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn 
545                 550                 555                 560 


Arg Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val 
                565                 570                 575     


Gly Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly 
            580                 585                 590         


Phe Ala Asp Ala Glu Gly Ser Phe Ile Leu Asp Ile Arg Asn Arg Asn 
        595                 600                 605             


Asn Glu Ser Asn Arg Tyr Arg Thr Ser Leu Arg Phe Gln Ile Thr Leu 
    610                 615                 620                 


His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys 
625                 630                 635                 640 


Val Gly Lys Ile Thr Asn Ser Ser Asp Arg Ala Val Met Leu Arg Val 
                645                 650                 655     


Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr 
            660                 665                 670         


Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala 
        675                 680                 685             


Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys 
    690                 695                 700                 


Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp Glu 
705                 710                 715                 720 


Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile 
                725                 730                 735     


Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ala Gly 
            740                 745                 750         


Glu Gly Tyr Phe Gly Val Asn Leu Lys Lys Val Lys Gly Thr Ala Lys 
        755                 760                 765             


Val Tyr Val Gly Leu Arg Phe Ser Ile Ser Gln His Ile Arg Asp Lys 
    770                 775                 780                 


Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Ser Ile Trp 
785                 790                 795                 800 


Glu Lys Asn Lys Ser Glu Phe Arg Trp Leu Glu Phe Val Val Thr Lys 
                805                 810                 815     


Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr 
            820                 825                 830         


Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala 
        835                 840                 845             


Lys Leu Ile Lys Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu 
    850                 855                 860                 


Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Gly Gly Gly Gly 
865                 870                 875                 880 


Ser Gly Gly Gly Gly Ser Ala Ser Ser Glu Pro Pro Arg Ala Glu Thr 
                885                 890                 895     


Phe Val Phe Leu Asp Leu Glu Ala Thr Gly Leu Pro Asn Met Asp Pro 
            900                 905                 910         


Glu Ile Ala Glu Ile Ser Leu Phe Ala Val His Arg Ser Ser Leu Glu 
        915                 920                 925             


Asn Pro Glu Arg Asp Asp Ser Gly Ser Leu Val Leu Pro Arg Val Leu 
    930                 935                 940                 


Asp Lys Leu Thr Leu Cys Met Cys Pro Glu Arg Pro Phe Thr Ala Lys 
945                 950                 955                 960 


Ala Ser Glu Ile Thr Gly Leu Ser Ser Glu Ser Leu Met His Cys Gly 
                965                 970                 975     


Lys Ala Gly Phe Asn Gly Ala Val Val Arg Thr Leu Gln Gly Phe Leu 
            980                 985                 990         


Ser Arg Gln Glu Gly Pro Ile Cys  Leu Val Ala His Asn  Gly Phe Asp 
        995                 1000                 1005             


Tyr Asp  Phe Pro Leu Leu Cys  Thr Glu Leu Gln Arg  Leu Gly Ala 
    1010                 1015                 1020             


His Leu  Pro Gln Asp Thr Val  Cys Leu Asp Thr Leu  Pro Ala Leu 
    1025                 1030                 1035             


Arg Gly  Leu Asp Arg Ala His  Ser His Gly Thr Arg  Ala Gln Gly 
    1040                 1045                 1050             


Arg Lys  Ser Tyr Ser Leu Ala  Ser Leu Phe His Arg  Tyr Phe Gln 
    1055                 1060                 1065             


Ala Glu  Pro Ser Ala Ala His  Ser Ala Glu Gly Asp  Val His Thr 
    1070                 1075                 1080             


Leu Leu  Leu Ile Phe Leu His  Arg Ala Pro Glu Leu  Leu Ala Trp 
    1085                 1090                 1095             


Ala Asp  Glu Gln Ala Arg Ser  Trp Ala His Ile Glu  Pro Met Tyr 
    1100                 1105                 1110             


Val Pro  Pro Asp Gly Pro Ser  Leu Glu Ala 
    1115                 1120             


<210>  35
<211>  3504
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-hsRad1 DNA construct

<400>  35
atgggatcct gcaggccacc taagaagaaa cgcaaagtcg tggatctacg cacgctcggc       60

tacagtcagc agcagcaaga gaagatcaaa ccgaaggtgc gttcgacagt ggcgcagcac      120

cacgaggcac tggtgggcca tgggtttaca cacgcgcaca tcgttgcgct cagccaacac      180

ccggcagcgt tagggaccgt cgctgtcacg tatcagcaca taatcacggc gttgccagag      240

gcgacacacg aagacatcgt tggcgtcggc aaacagtggt ccggcgcacg cgccctggag      300

gccttgctca cggatgcggg ggagttgaga ggtccgccgt tacagttgga cacaggccaa      360

cttgtgaaga ttgcaaaacg tggcggcgtg accgcaatgg aggcagtgca tgcatcgcgc      420

aatgcactga cgggtgcccc cctgaacctg accccggacc aagtggtggc tatcgccagc      480

aacattggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag      540

gaccatggcc tgaccccgga ccaagtggtg gctatcgcca gcaacattgg cggcaagcaa      600

gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg      660

gaccaagtgg tggctatcgc cagcaacatt ggcggcaagc aagcgctcga aacggtgcag      720

cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc      780

gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg      840

tgccaggacc atggcctgac tccggaccaa gtggtggcta tcgccagcca cgatggcggc      900

aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg      960

actccggacc aagtggtggc tatcgccagc cacgatggcg gcaagcaagc gctcgaaacg     1020

gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg     1080

gctatcgcca gcaacattgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg     1140

gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc cagcaacaat     1200

ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat     1260

ggcctgaccc cggaccaagt ggtggctatc gccagcaacg gtggcggcaa gcaagcgctc     1320

gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa     1380

gtggtggcta tcgccagcaa caatggcggc aagcaagcgc tcgaaacggt gcagcggctg     1440

ttgccggtgc tgtgccagga ccatggcctg accccggacc aagtggtggc tatcgccagc     1500

aacattggcg gcaagcaagc gctcgaaagc attgtggccc agctgagccg gcctgatccg     1560

gcgttggccg cgttgaccaa cgaccacctc gtcgccttgg cctgcctcgg cggacgtcct     1620

gccatggatg cagtgaaaaa gggattgccg cacgcgccgg aattgatcag aagagtcaat     1680

cgccgtattg gcgaacgcac gtcccatcgc gttgcgatat ctagagtggg aggaagctct     1740

cgcagagagt ccatcaaccc atggattctg actggtttcg ctgatgccga aggatcattc     1800

atactagaca tccgcaaccg aaacaacgaa agcaacagat accgaacttc gctgagattc     1860

cagatcaccc tgcacaacaa ggacaaatcg attctggaga atatccagtc cacttggaag     1920

gtcggcaaga tcacaaacag cagtgacaga gccgtcatgc tgagggtcac ccgtttcgaa     1980

gatttgaaag tgattatcga ccacttcgag aaatatccgc tgattaccca gaaattgggc     2040

gattacaagt tgtttaaaca ggcattcagc gtcatggaga ataaagaaca tcttaaggag     2100

aatgggatta aggagctcgt acgaatcaaa gctaagatga attggggtct caatgacgaa     2160

ttgaaaaaag catttccaga gaacattagc aaagagcgcc cccttatcaa taagaacatt     2220

ccgaatttca aatggctggc tggattcaca gctggtgaag gctacttcgg cgtgaatcta     2280

aaaaaggtaa agggcaccgc aaaggtatac gtgggactga gattctcaat ctcacagcac     2340

atcagagaca agaacctgat gaattcattg ataacatacc taggctgtgg ttccatctgg     2400

gagaagaaca agtctgagtt cagatggctc gagttcgtcg taaccaaatt cagcgatatc     2460

aacgacaaga tcattccggt attccaggaa aatactctga ttggcgtcaa actcgaggac     2520

tttgaagatt ggtgcaaggt tgccaaattg atcaaagaga agaaacacct gaccgaatcc     2580

ggtttggatg agattaagaa aatcaagctg aacatgaaca aaggtcgtgg tggaggcggt     2640

agcggaggcg gagggtcgcc gcttctgacc caacagatcc aagacgagga cgatcagtac     2700

agccttgttg ccagccttga caacgtgagg aatctctcca ctatcttgaa agctattcat     2760

ttccgagaac atgccacctg tttcgcaact aagaacggta tcaaagtaac agtggagaat     2820

gcaaagtgtg tgcaagcaaa tgccttcatt caggctggca tcttccaaga gttcaaagtt     2880

caggaagagt ctgttacttt ccgaatcaac ctaactgtcc tcttagactg tttatctatc     2940

ttcggatcaa gtccaatgcc agggacttta actgcactcc gaatgtgtta ccaaggttac     3000

ggataccctt tgatgctgtt cctcgaagaa ggaggagtgg tgacagtctg caagatcaat     3060

acacaggaac cagaggagac cctggacttc gatttctgct ccaccaatgt tatcaacaag     3120

atcattctcc agtcagaagg gctccgtgaa gcattctctg aattggatat gacgagtgaa     3180

gtcctacaga tcaccatgtc tcctgacaag ccttacttca ggttatctac tttcggaaat     3240

gcaggaagtt cccaccttga ctatcccaaa gattctgatt tgatggaggc atttcattgt     3300

aatcagactc aagtgaacag atacaagata tccctactga aaccctctac aaaggcatta     3360

gtcctatctt gtaaggtatc tattcggaca gataacagag gcttcctttc attacagtat     3420

atgattagaa atgaagatgg acaaatctgc tttgtggagt attactgctg ccctgatgag     3480

gaagttcctg aatctgagtc ttga                                            3504


<210>  36
<211>  3504
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-hsRad1 RNA construct

<400>  36
augggauccu gcaggccacc uaagaagaaa cgcaaagucg uggaucuacg cacgcucggc       60

uacagucagc agcagcaaga gaagaucaaa ccgaaggugc guucgacagu ggcgcagcac      120

cacgaggcac uggugggcca uggguuuaca cacgcgcaca ucguugcgcu cagccaacac      180

ccggcagcgu uagggaccgu cgcugucacg uaucagcaca uaaucacggc guugccagag      240

gcgacacacg aagacaucgu uggcgucggc aaacaguggu ccggcgcacg cgcccuggag      300

gccuugcuca cggaugcggg ggaguugaga gguccgccgu uacaguugga cacaggccaa      360

cuugugaaga uugcaaaacg uggcggcgug accgcaaugg aggcagugca ugcaucgcgc      420

aaugcacuga cgggugcccc ccugaaccug accccggacc aagugguggc uaucgccagc      480

aacauuggcg gcaagcaagc gcucgaaacg gugcagcggc uguugccggu gcugugccag      540

gaccauggcc ugaccccgga ccaaguggug gcuaucgcca gcaacauugg cggcaagcaa      600

gcgcucgaaa cggugcagcg gcuguugccg gugcugugcc aggaccaugg ccugaccccg      660

gaccaagugg uggcuaucgc cagcaacauu ggcggcaagc aagcgcucga aacggugcag      720

cggcuguugc cggugcugug ccaggaccau ggccugaccc cggaccaagu gguggcuauc      780

gccagcaacg guggcggcaa gcaagcgcuc gaaacggugc agcggcuguu gccggugcug      840

ugccaggacc auggccugac uccggaccaa gugguggcua ucgccagcca cgauggcggc      900

aagcaagcgc ucgaaacggu gcagcggcug uugccggugc ugugccagga ccauggccug      960

acuccggacc aagugguggc uaucgccagc cacgauggcg gcaagcaagc gcucgaaacg     1020

gugcagcggc uguugccggu gcugugccag gaccauggcc ugaccccgga ccaaguggug     1080

gcuaucgcca gcaacauugg cggcaagcaa gcgcucgaaa cggugcagcg gcuguugccg     1140

gugcugugcc aggaccaugg ccugaccccg gaccaagugg uggcuaucgc cagcaacaau     1200

ggcggcaagc aagcgcucga aacggugcag cggcuguugc cggugcugug ccaggaccau     1260

ggccugaccc cggaccaagu gguggcuauc gccagcaacg guggcggcaa gcaagcgcuc     1320

gaaacggugc agcggcuguu gccggugcug ugccaggacc auggccugac cccggaccaa     1380

gugguggcua ucgccagcaa caauggcggc aagcaagcgc ucgaaacggu gcagcggcug     1440

uugccggugc ugugccagga ccauggccug accccggacc aagugguggc uaucgccagc     1500

aacauuggcg gcaagcaagc gcucgaaagc auuguggccc agcugagccg gccugauccg     1560

gcguuggccg cguugaccaa cgaccaccuc gucgccuugg ccugccucgg cggacguccu     1620

gccauggaug cagugaaaaa gggauugccg cacgcgccgg aauugaucag aagagucaau     1680

cgccguauug gcgaacgcac gucccaucgc guugcgauau cuagaguggg aggaagcucu     1740

cgcagagagu ccaucaaccc auggauucug acugguuucg cugaugccga aggaucauuc     1800

auacuagaca uccgcaaccg aaacaacgaa agcaacagau accgaacuuc gcugagauuc     1860

cagaucaccc ugcacaacaa ggacaaaucg auucuggaga auauccaguc cacuuggaag     1920

gucggcaaga ucacaaacag cagugacaga gccgucaugc ugagggucac ccguuucgaa     1980

gauuugaaag ugauuaucga ccacuucgag aaauauccgc ugauuaccca gaaauugggc     2040

gauuacaagu uguuuaaaca ggcauucagc gucauggaga auaaagaaca ucuuaaggag     2100

aaugggauua aggagcucgu acgaaucaaa gcuaagauga auuggggucu caaugacgaa     2160

uugaaaaaag cauuuccaga gaacauuagc aaagagcgcc cccuuaucaa uaagaacauu     2220

ccgaauuuca aauggcuggc uggauucaca gcuggugaag gcuacuucgg cgugaaucua     2280

aaaaagguaa agggcaccgc aaagguauac gugggacuga gauucucaau cucacagcac     2340

aucagagaca agaaccugau gaauucauug auaacauacc uaggcugugg uuccaucugg     2400

gagaagaaca agucugaguu cagauggcuc gaguucgucg uaaccaaauu cagcgauauc     2460

aacgacaaga ucauuccggu auuccaggaa aauacucuga uuggcgucaa acucgaggac     2520

uuugaagauu ggugcaaggu ugccaaauug aucaaagaga agaaacaccu gaccgaaucc     2580

gguuuggaug agauuaagaa aaucaagcug aacaugaaca aaggucgugg uggaggcggu     2640

agcggaggcg gagggucgcc gcuucugacc caacagaucc aagacgagga cgaucaguac     2700

agccuuguug ccagccuuga caacgugagg aaucucucca cuaucuugaa agcuauucau     2760

uuccgagaac augccaccug uuucgcaacu aagaacggua ucaaaguaac aguggagaau     2820

gcaaagugug ugcaagcaaa ugccuucauu caggcuggca ucuuccaaga guucaaaguu     2880

caggaagagu cuguuacuuu ccgaaucaac cuaacugucc ucuuagacug uuuaucuauc     2940

uucggaucaa guccaaugcc agggacuuua acugcacucc gaauguguua ccaagguuac     3000

ggauacccuu ugaugcuguu ccucgaagaa ggaggagugg ugacagucug caagaucaau     3060

acacaggaac cagaggagac ccuggacuuc gauuucugcu ccaccaaugu uaucaacaag     3120

aucauucucc agucagaagg gcuccgugaa gcauucucug aauuggauau gacgagugaa     3180

guccuacaga ucaccauguc uccugacaag ccuuacuuca gguuaucuac uuucggaaau     3240

gcaggaaguu cccaccuuga cuaucccaaa gauucugauu ugauggaggc auuucauugu     3300

aaucagacuc aagugaacag auacaagaua ucccuacuga aacccucuac aaaggcauua     3360

guccuaucuu guaagguauc uauucggaca gauaacagag gcuuccuuuc auuacaguau     3420

augauuagaa augaagaugg acaaaucugc uuuguggagu auuacugcug cccugaugag     3480

gaaguuccug aaucugaguc uuga                                            3504


<210>  37
<211>  1167
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-hsRad1 protein 
       construct

<400>  37

Met Gly Ser Cys Arg Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser Ile Val 
            500                 505                 510         


Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp 
        515                 520                 525             


His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala 
    530                 535                 540                 


Val Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn 
545                 550                 555                 560 


Arg Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val 
                565                 570                 575     


Gly Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly 
            580                 585                 590         


Phe Ala Asp Ala Glu Gly Ser Phe Ile Leu Asp Ile Arg Asn Arg Asn 
        595                 600                 605             


Asn Glu Ser Asn Arg Tyr Arg Thr Ser Leu Arg Phe Gln Ile Thr Leu 
    610                 615                 620                 


His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys 
625                 630                 635                 640 


Val Gly Lys Ile Thr Asn Ser Ser Asp Arg Ala Val Met Leu Arg Val 
                645                 650                 655     


Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr 
            660                 665                 670         


Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala 
        675                 680                 685             


Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys 
    690                 695                 700                 


Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp Glu 
705                 710                 715                 720 


Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile 
                725                 730                 735     


Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ala Gly 
            740                 745                 750         


Glu Gly Tyr Phe Gly Val Asn Leu Lys Lys Val Lys Gly Thr Ala Lys 
        755                 760                 765             


Val Tyr Val Gly Leu Arg Phe Ser Ile Ser Gln His Ile Arg Asp Lys 
    770                 775                 780                 


Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Ser Ile Trp 
785                 790                 795                 800 


Glu Lys Asn Lys Ser Glu Phe Arg Trp Leu Glu Phe Val Val Thr Lys 
                805                 810                 815     


Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr 
            820                 825                 830         


Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala 
        835                 840                 845             


Lys Leu Ile Lys Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu 
    850                 855                 860                 


Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Gly Gly Gly Gly 
865                 870                 875                 880 


Ser Gly Gly Gly Gly Ser Pro Leu Leu Thr Gln Gln Ile Gln Asp Glu 
                885                 890                 895     


Asp Asp Gln Tyr Ser Leu Val Ala Ser Leu Asp Asn Val Arg Asn Leu 
            900                 905                 910         


Ser Thr Ile Leu Lys Ala Ile His Phe Arg Glu His Ala Thr Cys Phe 
        915                 920                 925             


Ala Thr Lys Asn Gly Ile Lys Val Thr Val Glu Asn Ala Lys Cys Val 
    930                 935                 940                 


Gln Ala Asn Ala Phe Ile Gln Ala Gly Ile Phe Gln Glu Phe Lys Val 
945                 950                 955                 960 


Gln Glu Glu Ser Val Thr Phe Arg Ile Asn Leu Thr Val Leu Leu Asp 
                965                 970                 975     


Cys Leu Ser Ile Phe Gly Ser Ser Pro Met Pro Gly Thr Leu Thr Ala 
            980                 985                 990         


Leu Arg Met Cys Tyr Gln Gly Tyr  Gly Tyr Pro Leu Met  Leu Phe Leu 
        995                 1000                 1005             


Glu Glu  Gly Gly Val Val Thr  Val Cys Lys Ile Asn  Thr Gln Glu 
    1010                 1015                 1020             


Pro Glu  Glu Thr Leu Asp Phe  Asp Phe Cys Ser Thr  Asn Val Ile 
    1025                 1030                 1035             


Asn Lys  Ile Ile Leu Gln Ser  Glu Gly Leu Arg Glu  Ala Phe Ser 
    1040                 1045                 1050             


Glu Leu  Asp Met Thr Ser Glu  Val Leu Gln Ile Thr  Met Ser Pro 
    1055                 1060                 1065             


Asp Lys  Pro Tyr Phe Arg Leu  Ser Thr Phe Gly Asn  Ala Gly Ser 
    1070                 1075                 1080             


Ser His  Leu Asp Tyr Pro Lys  Asp Ser Asp Leu Met  Glu Ala Phe 
    1085                 1090                 1095             


His Cys  Asn Gln Thr Gln Val  Asn Arg Tyr Lys Ile  Ser Leu Leu 
    1100                 1105                 1110             


Lys Pro  Ser Thr Lys Ala Leu  Val Leu Ser Cys Lys  Val Ser Ile 
    1115                 1120                 1125             


Arg Thr  Asp Asn Arg Gly Phe  Leu Ser Leu Gln Tyr  Met Ile Arg 
    1130                 1135                 1140             


Asn Glu  Asp Gly Gln Ile Cys  Phe Val Glu Tyr Tyr  Cys Cys Pro 
    1145                 1150                 1155             


Asp Glu  Glu Val Pro Glu Ser  Glu Ser 
    1160                 1165         


<210>  38
<211>  3870
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-hsRad9a DNA construct

<400>  38
atgggatcct gcaggccacc taagaagaaa cgcaaagtcg tggatctacg cacgctcggc       60

tacagtcagc agcagcaaga gaagatcaaa ccgaaggtgc gttcgacagt ggcgcagcac      120

cacgaggcac tggtgggcca tgggtttaca cacgcgcaca tcgttgcgct cagccaacac      180

ccggcagcgt tagggaccgt cgctgtcacg tatcagcaca taatcacggc gttgccagag      240

gcgacacacg aagacatcgt tggcgtcggc aaacagtggt ccggcgcacg cgccctggag      300

gccttgctca cggatgcggg ggagttgaga ggtccgccgt tacagttgga cacaggccaa      360

cttgtgaaga ttgcaaaacg tggcggcgtg accgcaatgg aggcagtgca tgcatcgcgc      420

aatgcactga cgggtgcccc cctgaacctg accccggacc aagtggtggc tatcgccagc      480

aacattggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag      540

gaccatggcc tgaccccgga ccaagtggtg gctatcgcca gcaacattgg cggcaagcaa      600

gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg      660

gaccaagtgg tggctatcgc cagcaacatt ggcggcaagc aagcgctcga aacggtgcag      720

cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc      780

gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg      840

tgccaggacc atggcctgac tccggaccaa gtggtggcta tcgccagcca cgatggcggc      900

aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg      960

actccggacc aagtggtggc tatcgccagc cacgatggcg gcaagcaagc gctcgaaacg     1020

gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg     1080

gctatcgcca gcaacattgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg     1140

gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc cagcaacaat     1200

ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat     1260

ggcctgaccc cggaccaagt ggtggctatc gccagcaacg gtggcggcaa gcaagcgctc     1320

gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa     1380

gtggtggcta tcgccagcaa caatggcggc aagcaagcgc tcgaaacggt gcagcggctg     1440

ttgccggtgc tgtgccagga ccatggcctg accccggacc aagtggtggc tatcgccagc     1500

aacattggcg gcaagcaagc gctcgaaagc attgtggccc agctgagccg gcctgatccg     1560

gcgttggccg cgttgaccaa cgaccacctc gtcgccttgg cctgcctcgg cggacgtcct     1620

gccatggatg cagtgaaaaa gggattgccg cacgcgccgg aattgatcag aagagtcaat     1680

cgccgtattg gcgaacgcac gtcccatcgc gttgcgatat ctagagtggg aggaagctct     1740

cgcagagagt ccatcaaccc atggattctg actggtttcg ctgatgccga aggatcattc     1800

atactagaca tccgcaaccg aaacaacgaa agcaacagat accgaacttc gctgagattc     1860

cagatcaccc tgcacaacaa ggacaaatcg attctggaga atatccagtc cacttggaag     1920

gtcggcaaga tcacaaacag cagtgacaga gccgtcatgc tgagggtcac ccgtttcgaa     1980

gatttgaaag tgattatcga ccacttcgag aaatatccgc tgattaccca gaaattgggc     2040

gattacaagt tgtttaaaca ggcattcagc gtcatggaga ataaagaaca tcttaaggag     2100

aatgggatta aggagctcgt acgaatcaaa gctaagatga attggggtct caatgacgaa     2160

ttgaaaaaag catttccaga gaacattagc aaagagcgcc cccttatcaa taagaacatt     2220

ccgaatttca aatggctggc tggattcaca gctggtgaag gctacttcgg cgtgaatcta     2280

aaaaaggtaa agggcaccgc aaaggtatac gtgggactga gattctcaat ctcacagcac     2340

atcagagaca agaacctgat gaattcattg ataacatacc taggctgtgg ttccatctgg     2400

gagaagaaca agtctgagtt cagatggctc gagttcgtcg taaccaaatt cagcgatatc     2460

aacgacaaga tcattccggt attccaggaa aatactctga ttggcgtcaa actcgaggac     2520

tttgaagatt ggtgcaaggt tgccaaattg atcaaagaga agaaacacct gaccgaatcc     2580

ggtttggatg agattaagaa aatcaagctg aacatgaaca aaggtcgtgg tggaggcggt     2640

agcggaggcg gagggtcggc tagcggcaaa ggagggttct tcagcaggtg gtggcggagc     2700

gggaggacga tagggcaagt gtgtgagcag aagcagccag agggctgggt ctgtggctgc     2760

cctctgacca cgtccctctc cctgctcttc gtggcccaac tctccctccg aacggtgaac     2820

tcctcccgct ctgcctatgc ctgctttctc tttgctccgc tcttcttcca gcaataccag     2880

gcagccactc ctggtcaaga cctgctgcga tgtaagattc tgatgaagtc tttcctgtct     2940

gtcttccgct cactggcgat gctggagaag acggtggaga agtgctgcat ctccctgaat     3000

ggtcggagca gtcgcctggt agtccaactg cattgcaagt tcggcgtgcg gaagactcac     3060

aacctgtcct tccaggactg tgagtccctt caggccgtct ttgacccagc ctcgtgccct     3120

cacatgctcc gagcaccagc acgggttctg ggagaggctg ttctgccctt ctctcctgca     3180

ctggctgaag tgacgctggg cattggccgt ggacgcaggg tcatcctgcg tagttaccac     3240

gaggaggagg cagacagcac tgccaaagcg atggtgactg agatgtgcct tggagaggag     3300

gatttccagc aactacaggc ccaggaagga gttgccatca ctttctgcct caaggagttc     3360

cgagggctcc tgagtttcgc agagtcagca aacttgaatc ttagcattca tttcgatgct     3420

ccaggcaggc cagccatctt caccatcaag gactctttgc tggacggcca ctttgtctta     3480

gccacactct cagacaccga ctcgcactcc caagacctgg gctcaccaga gcgtcaccag     3540

ccagtgcctc aactccaggc tcacagcaca cctcaccctg acgactttgc caatgacgac     3600

attgactctt acatgatagc gatggaaacc actataggca atgagggctc gcgtgtgctg     3660

ccctccattt ccctttcacc tggccctcag cctcccaaga gcccaggacc tcactccgag     3720

gaggaagatg aggctgagcc cagcacagtg cctgggactc ctccacccaa gaagttccgc     3780

tcactgttct tcggctccat cctggctcct gttcgctcac cacagggtcc tagccctgtg     3840

ctggcggaag acagtgaggg tgaaggctga                                      3870


<210>  39
<211>  3870
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-hsRad9a RNA construct

<400>  39
augggauccu gcaggccacc uaagaagaaa cgcaaagucg uggaucuacg cacgcucggc       60

uacagucagc agcagcaaga gaagaucaaa ccgaaggugc guucgacagu ggcgcagcac      120

cacgaggcac uggugggcca uggguuuaca cacgcgcaca ucguugcgcu cagccaacac      180

ccggcagcgu uagggaccgu cgcugucacg uaucagcaca uaaucacggc guugccagag      240

gcgacacacg aagacaucgu uggcgucggc aaacaguggu ccggcgcacg cgcccuggag      300

gccuugcuca cggaugcggg ggaguugaga gguccgccgu uacaguugga cacaggccaa      360

cuugugaaga uugcaaaacg uggcggcgug accgcaaugg aggcagugca ugcaucgcgc      420

aaugcacuga cgggugcccc ccugaaccug accccggacc aagugguggc uaucgccagc      480

aacauuggcg gcaagcaagc gcucgaaacg gugcagcggc uguugccggu gcugugccag      540

gaccauggcc ugaccccgga ccaaguggug gcuaucgcca gcaacauugg cggcaagcaa      600

gcgcucgaaa cggugcagcg gcuguugccg gugcugugcc aggaccaugg ccugaccccg      660

gaccaagugg uggcuaucgc cagcaacauu ggcggcaagc aagcgcucga aacggugcag      720

cggcuguugc cggugcugug ccaggaccau ggccugaccc cggaccaagu gguggcuauc      780

gccagcaacg guggcggcaa gcaagcgcuc gaaacggugc agcggcuguu gccggugcug      840

ugccaggacc auggccugac uccggaccaa gugguggcua ucgccagcca cgauggcggc      900

aagcaagcgc ucgaaacggu gcagcggcug uugccggugc ugugccagga ccauggccug      960

acuccggacc aagugguggc uaucgccagc cacgauggcg gcaagcaagc gcucgaaacg     1020

gugcagcggc uguugccggu gcugugccag gaccauggcc ugaccccgga ccaaguggug     1080

gcuaucgcca gcaacauugg cggcaagcaa gcgcucgaaa cggugcagcg gcuguugccg     1140

gugcugugcc aggaccaugg ccugaccccg gaccaagugg uggcuaucgc cagcaacaau     1200

ggcggcaagc aagcgcucga aacggugcag cggcuguugc cggugcugug ccaggaccau     1260

ggccugaccc cggaccaagu gguggcuauc gccagcaacg guggcggcaa gcaagcgcuc     1320

gaaacggugc agcggcuguu gccggugcug ugccaggacc auggccugac cccggaccaa     1380

gugguggcua ucgccagcaa caauggcggc aagcaagcgc ucgaaacggu gcagcggcug     1440

uugccggugc ugugccagga ccauggccug accccggacc aagugguggc uaucgccagc     1500

aacauuggcg gcaagcaagc gcucgaaagc auuguggccc agcugagccg gccugauccg     1560

gcguuggccg cguugaccaa cgaccaccuc gucgccuugg ccugccucgg cggacguccu     1620

gccauggaug cagugaaaaa gggauugccg cacgcgccgg aauugaucag aagagucaau     1680

cgccguauug gcgaacgcac gucccaucgc guugcgauau cuagaguggg aggaagcucu     1740

cgcagagagu ccaucaaccc auggauucug acugguuucg cugaugccga aggaucauuc     1800

auacuagaca uccgcaaccg aaacaacgaa agcaacagau accgaacuuc gcugagauuc     1860

cagaucaccc ugcacaacaa ggacaaaucg auucuggaga auauccaguc cacuuggaag     1920

gucggcaaga ucacaaacag cagugacaga gccgucaugc ugagggucac ccguuucgaa     1980

gauuugaaag ugauuaucga ccacuucgag aaauauccgc ugauuaccca gaaauugggc     2040

gauuacaagu uguuuaaaca ggcauucagc gucauggaga auaaagaaca ucuuaaggag     2100

aaugggauua aggagcucgu acgaaucaaa gcuaagauga auuggggucu caaugacgaa     2160

uugaaaaaag cauuuccaga gaacauuagc aaagagcgcc cccuuaucaa uaagaacauu     2220

ccgaauuuca aauggcuggc uggauucaca gcuggugaag gcuacuucgg cgugaaucua     2280

aaaaagguaa agggcaccgc aaagguauac gugggacuga gauucucaau cucacagcac     2340

aucagagaca agaaccugau gaauucauug auaacauacc uaggcugugg uuccaucugg     2400

gagaagaaca agucugaguu cagauggcuc gaguucgucg uaaccaaauu cagcgauauc     2460

aacgacaaga ucauuccggu auuccaggaa aauacucuga uuggcgucaa acucgaggac     2520

uuugaagauu ggugcaaggu ugccaaauug aucaaagaga agaaacaccu gaccgaaucc     2580

gguuuggaug agauuaagaa aaucaagcug aacaugaaca aaggucgugg uggaggcggu     2640

agcggaggcg gagggucggc uagcggcaaa ggaggguucu ucagcaggug guggcggagc     2700

gggaggacga uagggcaagu gugugagcag aagcagccag agggcugggu cuguggcugc     2760

ccucugacca cgucccucuc ccugcucuuc guggcccaac ucucccuccg aacggugaac     2820

uccucccgcu cugccuaugc cugcuuucuc uuugcuccgc ucuucuucca gcaauaccag     2880

gcagccacuc cuggucaaga ccugcugcga uguaagauuc ugaugaaguc uuuccugucu     2940

gucuuccgcu cacuggcgau gcuggagaag acgguggaga agugcugcau cucccugaau     3000

ggucggagca gucgccuggu aguccaacug cauugcaagu ucggcgugcg gaagacucac     3060

aaccuguccu uccaggacug ugagucccuu caggccgucu uugacccagc cucgugcccu     3120

cacaugcucc gagcaccagc acggguucug ggagaggcug uucugcccuu cucuccugca     3180

cuggcugaag ugacgcuggg cauuggccgu ggacgcaggg ucauccugcg uaguuaccac     3240

gaggaggagg cagacagcac ugccaaagcg auggugacug agaugugccu uggagaggag     3300

gauuuccagc aacuacaggc ccaggaagga guugccauca cuuucugccu caaggaguuc     3360

cgagggcucc ugaguuucgc agagucagca aacuugaauc uuagcauuca uuucgaugcu     3420

ccaggcaggc cagccaucuu caccaucaag gacucuuugc uggacggcca cuuugucuua     3480

gccacacucu cagacaccga cucgcacucc caagaccugg gcucaccaga gcgucaccag     3540

ccagugccuc aacuccaggc ucacagcaca ccucacccug acgacuuugc caaugacgac     3600

auugacucuu acaugauagc gauggaaacc acuauaggca augagggcuc gcgugugcug     3660

cccuccauuu cccuuucacc uggcccucag ccucccaaga gcccaggacc ucacuccgag     3720

gaggaagaug aggcugagcc cagcacagug ccugggacuc cuccacccaa gaaguuccgc     3780

ucacuguucu ucggcuccau ccuggcuccu guucgcucac cacagggucc uagcccugug     3840

cuggcggaag acagugaggg ugaaggcuga                                      3870


<210>  40
<211>  1289
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-hsRad9a protein 
       construct

<400>  40

Met Gly Ser Cys Arg Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser Ile Val 
            500                 505                 510         


Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp 
        515                 520                 525             


His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala 
    530                 535                 540                 


Val Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn 
545                 550                 555                 560 


Arg Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val 
                565                 570                 575     


Gly Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly 
            580                 585                 590         


Phe Ala Asp Ala Glu Gly Ser Phe Ile Leu Asp Ile Arg Asn Arg Asn 
        595                 600                 605             


Asn Glu Ser Asn Arg Tyr Arg Thr Ser Leu Arg Phe Gln Ile Thr Leu 
    610                 615                 620                 


His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys 
625                 630                 635                 640 


Val Gly Lys Ile Thr Asn Ser Ser Asp Arg Ala Val Met Leu Arg Val 
                645                 650                 655     


Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr 
            660                 665                 670         


Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala 
        675                 680                 685             


Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys 
    690                 695                 700                 


Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp Glu 
705                 710                 715                 720 


Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile 
                725                 730                 735     


Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ala Gly 
            740                 745                 750         


Glu Gly Tyr Phe Gly Val Asn Leu Lys Lys Val Lys Gly Thr Ala Lys 
        755                 760                 765             


Val Tyr Val Gly Leu Arg Phe Ser Ile Ser Gln His Ile Arg Asp Lys 
    770                 775                 780                 


Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Ser Ile Trp 
785                 790                 795                 800 


Glu Lys Asn Lys Ser Glu Phe Arg Trp Leu Glu Phe Val Val Thr Lys 
                805                 810                 815     


Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr 
            820                 825                 830         


Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala 
        835                 840                 845             


Lys Leu Ile Lys Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu 
    850                 855                 860                 


Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Gly Gly Gly Gly 
865                 870                 875                 880 


Ser Gly Gly Gly Gly Ser Ala Ser Gly Lys Gly Gly Phe Phe Ser Arg 
                885                 890                 895     


Trp Trp Arg Ser Gly Arg Thr Ile Gly Gln Val Cys Glu Gln Lys Gln 
            900                 905                 910         


Pro Glu Gly Trp Val Cys Gly Cys Pro Leu Thr Thr Ser Leu Ser Leu 
        915                 920                 925             


Leu Phe Val Ala Gln Leu Ser Leu Arg Thr Val Asn Ser Ser Arg Ser 
    930                 935                 940                 


Ala Tyr Ala Cys Phe Leu Phe Ala Pro Leu Phe Phe Gln Gln Tyr Gln 
945                 950                 955                 960 


Ala Ala Thr Pro Gly Gln Asp Leu Leu Arg Cys Lys Ile Leu Met Lys 
                965                 970                 975     


Ser Phe Leu Ser Val Phe Arg Ser Leu Ala Met Leu Glu Lys Thr Val 
            980                 985                 990         


Glu Lys Cys Cys Ile Ser Leu Asn  Gly Arg Ser Ser Arg  Leu Val Val 
        995                 1000                 1005             


Gln Leu  His Cys Lys Phe Gly  Val Arg Lys Thr His  Asn Leu Ser 
    1010                 1015                 1020             


Phe Gln  Asp Cys Glu Ser Leu  Gln Ala Val Phe Asp  Pro Ala Ser 
    1025                 1030                 1035             


Cys Pro  His Met Leu Arg Ala  Pro Ala Arg Val Leu  Gly Glu Ala 
    1040                 1045                 1050             


Val Leu  Pro Phe Ser Pro Ala  Leu Ala Glu Val Thr  Leu Gly Ile 
    1055                 1060                 1065             


Gly Arg  Gly Arg Arg Val Ile  Leu Arg Ser Tyr His  Glu Glu Glu 
    1070                 1075                 1080             


Ala Asp  Ser Thr Ala Lys Ala  Met Val Thr Glu Met  Cys Leu Gly 
    1085                 1090                 1095             


Glu Glu  Asp Phe Gln Gln Leu  Gln Ala Gln Glu Gly  Val Ala Ile 
    1100                 1105                 1110             


Thr Phe  Cys Leu Lys Glu Phe  Arg Gly Leu Leu Ser  Phe Ala Glu 
    1115                 1120                 1125             


Ser Ala  Asn Leu Asn Leu Ser  Ile His Phe Asp Ala  Pro Gly Arg 
    1130                 1135                 1140             


Pro Ala  Ile Phe Thr Ile Lys  Asp Ser Leu Leu Asp  Gly His Phe 
    1145                 1150                 1155             


Val Leu  Ala Thr Leu Ser Asp  Thr Asp Ser His Ser  Gln Asp Leu 
    1160                 1165                 1170             


Gly Ser  Pro Glu Arg His Gln  Pro Val Pro Gln Leu  Gln Ala His 
    1175                 1180                 1185             


Ser Thr  Pro His Pro Asp Asp  Phe Ala Asn Asp Asp  Ile Asp Ser 
    1190                 1195                 1200             


Tyr Met  Ile Ala Met Glu Thr  Thr Ile Gly Asn Glu  Gly Ser Arg 
    1205                 1210                 1215             


Val Leu  Pro Ser Ile Ser Leu  Ser Pro Gly Pro Gln  Pro Pro Lys 
    1220                 1225                 1230             


Ser Pro  Gly Pro His Ser Glu  Glu Glu Asp Glu Ala  Glu Pro Ser 
    1235                 1240                 1245             


Thr Val  Pro Gly Thr Pro Pro  Pro Lys Lys Phe Arg  Ser Leu Phe 
    1250                 1255                 1260             


Phe Gly  Ser Ile Leu Ala Pro  Val Arg Ser Pro Gln  Gly Pro Ser 
    1265                 1270                 1275             


Pro Val  Leu Ala Glu Asp Ser  Glu Gly Glu Gly 
    1280                 1285                 


<210>  41
<211>  4086
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-ExoI DNA construct

<400>  41
atgggatcct gcaggccacc taagaagaaa cgcaaagtcg tggatctacg cacgctcggc       60

tacagtcagc agcagcaaga gaagatcaaa ccgaaggtgc gttcgacagt ggcgcagcac      120

cacgaggcac tggtgggcca tgggtttaca cacgcgcaca tcgttgcgct cagccaacac      180

ccggcagcgt tagggaccgt cgctgtcacg tatcagcaca taatcacggc gttgccagag      240

gcgacacacg aagacatcgt tggcgtcggc aaacagtggt ccggcgcacg cgccctggag      300

gccttgctca cggatgcggg ggagttgaga ggtccgccgt tacagttgga cacaggccaa      360

cttgtgaaga ttgcaaaacg tggcggcgtg accgcaatgg aggcagtgca tgcatcgcgc      420

aatgcactga cgggtgcccc cctgaacctg accccggacc aagtggtggc tatcgccagc      480

aacattggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag      540

gaccatggcc tgaccccgga ccaagtggtg gctatcgcca gcaacattgg cggcaagcaa      600

gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg      660

gaccaagtgg tggctatcgc cagcaacatt ggcggcaagc aagcgctcga aacggtgcag      720

cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc      780

gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg      840

tgccaggacc atggcctgac tccggaccaa gtggtggcta tcgccagcca cgatggcggc      900

aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg      960

actccggacc aagtggtggc tatcgccagc cacgatggcg gcaagcaagc gctcgaaacg     1020

gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg     1080

gctatcgcca gcaacattgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg     1140

gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc cagcaacaat     1200

ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat     1260

ggcctgaccc cggaccaagt ggtggctatc gccagcaacg gtggcggcaa gcaagcgctc     1320

gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa     1380

gtggtggcta tcgccagcaa caatggcggc aagcaagcgc tcgaaacggt gcagcggctg     1440

ttgccggtgc tgtgccagga ccatggcctg accccggacc aagtggtggc tatcgccagc     1500

aacattggcg gcaagcaagc gctcgaaagc attgtggccc agctgagccg gcctgatccg     1560

gcgttggccg cgttgaccaa cgaccacctc gtcgccttgg cctgcctcgg cggacgtcct     1620

gccatggatg cagtgaaaaa gggattgccg cacgcgccgg aattgatcag aagagtcaat     1680

cgccgtattg gcgaacgcac gtcccatcgc gttgcgatat ctagagtggg aggaagctct     1740

cgcagagagt ccatcaaccc atggattctg actggtttcg ctgatgccga aggatcattc     1800

atactagaca tccgcaaccg aaacaacgaa agcaacagat accgaacttc gctgagattc     1860

cagatcaccc tgcacaacaa ggacaaatcg attctggaga atatccagtc cacttggaag     1920

gtcggcaaga tcacaaacag cagtgacaga gccgtcatgc tgagggtcac ccgtttcgaa     1980

gatttgaaag tgattatcga ccacttcgag aaatatccgc tgattaccca gaaattgggc     2040

gattacaagt tgtttaaaca ggcattcagc gtcatggaga ataaagaaca tcttaaggag     2100

aatgggatta aggagctcgt acgaatcaaa gctaagatga attggggtct caatgacgaa     2160

ttgaaaaaag catttccaga gaacattagc aaagagcgcc cccttatcaa taagaacatt     2220

ccgaatttca aatggctggc tggattcaca gctggtgaag gctacttcgg cgtgaatcta     2280

aaaaaggtaa agggcaccgc aaaggtatac gtgggactga gattctcaat ctcacagcac     2340

atcagagaca agaacctgat gaattcattg ataacatacc taggctgtgg ttccatctgg     2400

gagaagaaca agtctgagtt cagatggctc gagttcgtcg taaccaaatt cagcgatatc     2460

aacgacaaga tcattccggt attccaggaa aatactctga ttggcgtcaa actcgaggac     2520

tttgaagatt ggtgcaaggt tgccaaattg atcaaagaga agaaacacct gaccgaatcc     2580

ggtttggatg agattaagaa aatcaagctg aacatgaaca aaggtcgtgg tggaggcggt     2640

agcggaggcg gagggtcggc tagcaacgat ggcaagcagc aaagtacatt ccttttccac     2700

gactacgaaa cctttggaac ccaccccgct ctggatcggc cagcacagtt cgctgccata     2760

agaaccgaca acgagttcaa tgtgatcggt gaacccgagg tgttttactg caaacctgcg     2820

gacgattatc tgcctcaacc gggagccgtt ctgattacgg gaattacccc ccaagaagcg     2880

cgtgccaagg gcgaaaatga agctgcattt gccgctagaa tccatagcct gtttactgtt     2940

ccgaagacgt gtatcttggg atacaacaat gtacgctttg atgacgaagt aactcgtaac     3000

gtgttctatc ggaacttcta tgacccttac gcctggagtt ggcagcacga caattctcga     3060

tgggatcttc tggatgtgat gagagcctgc tacgctctca ggccagaggg cattaactgg     3120

cctgagaacg acgatgggct tccgtcattt cgccttgagc atcttaccaa agcgaatggc     3180

atcgaacact ccaatgcaca cgacgctatg gctgatgtct acgcgactat tgcaatggcg     3240

aaattggtca agacaagaca gccgaggttg tttgactacc tgtttacgca tcgaaacaaa     3300

cataagctca tggcactgat agatgtccct caaatgaagc ccctcgttca cgtcagcggt     3360

atgttcggag cgtggagagg aaacacgtcc tgggttgctc ctctggcatg gcatccagag     3420

aacagaaacg cggtaatcat ggtcgatctc gcaggagaca ttagcccact tctcgaattg     3480

gattccgaca ccttgcgaga acgcctttac acagcgaaaa ctgatttggg tgataacgct     3540

gccgtacctg tcaaattggt acatatcaac aaatgtccgg tgctcgcgca agccaacacc     3600

ctgagacctg aagacgcgga tagactgggg ataaaccggc agcactgttt ggataatctc     3660

aagatactca gggagaatcc gcaggtgcgc gaaaaagtcg tagccatctt tgcggaggca     3720

gaaccattta cgccgtctga caatgtagat gctcagttat acaatggctt ctttagtgac     3780

gctgaccgag ctgcgatgaa gattgtgcta gagactgaac cgagaaacct ctctgcattg     3840

gacatcactt ttgtcgataa gcgaatagag aagttgctgt tcaactatcg tgcccgaaac     3900

tttccaggga cactcgatta cgcagagcaa caaaggtggc ttgagcatcg caggcaggtg     3960

tttaccccag agttcttgca gggttacgct gaggagattc aaatgttggc acagcagtat     4020

gcagatgata aggagaaagt cgcgttgctg aaggctctgt ggcaatacgc cgaagagata     4080

gtgtga                                                                4086


<210>  42
<211>  4086
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-ExoI RNA construct

<400>  42
augggauccu gcaggccacc uaagaagaaa cgcaaagucg uggaucuacg cacgcucggc       60

uacagucagc agcagcaaga gaagaucaaa ccgaaggugc guucgacagu ggcgcagcac      120

cacgaggcac uggugggcca uggguuuaca cacgcgcaca ucguugcgcu cagccaacac      180

ccggcagcgu uagggaccgu cgcugucacg uaucagcaca uaaucacggc guugccagag      240

gcgacacacg aagacaucgu uggcgucggc aaacaguggu ccggcgcacg cgcccuggag      300

gccuugcuca cggaugcggg ggaguugaga gguccgccgu uacaguugga cacaggccaa      360

cuugugaaga uugcaaaacg uggcggcgug accgcaaugg aggcagugca ugcaucgcgc      420

aaugcacuga cgggugcccc ccugaaccug accccggacc aagugguggc uaucgccagc      480

aacauuggcg gcaagcaagc gcucgaaacg gugcagcggc uguugccggu gcugugccag      540

gaccauggcc ugaccccgga ccaaguggug gcuaucgcca gcaacauugg cggcaagcaa      600

gcgcucgaaa cggugcagcg gcuguugccg gugcugugcc aggaccaugg ccugaccccg      660

gaccaagugg uggcuaucgc cagcaacauu ggcggcaagc aagcgcucga aacggugcag      720

cggcuguugc cggugcugug ccaggaccau ggccugaccc cggaccaagu gguggcuauc      780

gccagcaacg guggcggcaa gcaagcgcuc gaaacggugc agcggcuguu gccggugcug      840

ugccaggacc auggccugac uccggaccaa gugguggcua ucgccagcca cgauggcggc      900

aagcaagcgc ucgaaacggu gcagcggcug uugccggugc ugugccagga ccauggccug      960

acuccggacc aagugguggc uaucgccagc cacgauggcg gcaagcaagc gcucgaaacg     1020

gugcagcggc uguugccggu gcugugccag gaccauggcc ugaccccgga ccaaguggug     1080

gcuaucgcca gcaacauugg cggcaagcaa gcgcucgaaa cggugcagcg gcuguugccg     1140

gugcugugcc aggaccaugg ccugaccccg gaccaagugg uggcuaucgc cagcaacaau     1200

ggcggcaagc aagcgcucga aacggugcag cggcuguugc cggugcugug ccaggaccau     1260

ggccugaccc cggaccaagu gguggcuauc gccagcaacg guggcggcaa gcaagcgcuc     1320

gaaacggugc agcggcuguu gccggugcug ugccaggacc auggccugac cccggaccaa     1380

gugguggcua ucgccagcaa caauggcggc aagcaagcgc ucgaaacggu gcagcggcug     1440

uugccggugc ugugccagga ccauggccug accccggacc aagugguggc uaucgccagc     1500

aacauuggcg gcaagcaagc gcucgaaagc auuguggccc agcugagccg gccugauccg     1560

gcguuggccg cguugaccaa cgaccaccuc gucgccuugg ccugccucgg cggacguccu     1620

gccauggaug cagugaaaaa gggauugccg cacgcgccgg aauugaucag aagagucaau     1680

cgccguauug gcgaacgcac gucccaucgc guugcgauau cuagaguggg aggaagcucu     1740

cgcagagagu ccaucaaccc auggauucug acugguuucg cugaugccga aggaucauuc     1800

auacuagaca uccgcaaccg aaacaacgaa agcaacagau accgaacuuc gcugagauuc     1860

cagaucaccc ugcacaacaa ggacaaaucg auucuggaga auauccaguc cacuuggaag     1920

gucggcaaga ucacaaacag cagugacaga gccgucaugc ugagggucac ccguuucgaa     1980

gauuugaaag ugauuaucga ccacuucgag aaauauccgc ugauuaccca gaaauugggc     2040

gauuacaagu uguuuaaaca ggcauucagc gucauggaga auaaagaaca ucuuaaggag     2100

aaugggauua aggagcucgu acgaaucaaa gcuaagauga auuggggucu caaugacgaa     2160

uugaaaaaag cauuuccaga gaacauuagc aaagagcgcc cccuuaucaa uaagaacauu     2220

ccgaauuuca aauggcuggc uggauucaca gcuggugaag gcuacuucgg cgugaaucua     2280

aaaaagguaa agggcaccgc aaagguauac gugggacuga gauucucaau cucacagcac     2340

aucagagaca agaaccugau gaauucauug auaacauacc uaggcugugg uuccaucugg     2400

gagaagaaca agucugaguu cagauggcuc gaguucgucg uaaccaaauu cagcgauauc     2460

aacgacaaga ucauuccggu auuccaggaa aauacucuga uuggcgucaa acucgaggac     2520

uuugaagauu ggugcaaggu ugccaaauug aucaaagaga agaaacaccu gaccgaaucc     2580

gguuuggaug agauuaagaa aaucaagcug aacaugaaca aaggucgugg uggaggcggu     2640

agcggaggcg gagggucggc uagcaacgau ggcaagcagc aaaguacauu ccuuuuccac     2700

gacuacgaaa ccuuuggaac ccaccccgcu cuggaucggc cagcacaguu cgcugccaua     2760

agaaccgaca acgaguucaa ugugaucggu gaacccgagg uguuuuacug caaaccugcg     2820

gacgauuauc ugccucaacc gggagccguu cugauuacgg gaauuacccc ccaagaagcg     2880

cgugccaagg gcgaaaauga agcugcauuu gccgcuagaa uccauagccu guuuacuguu     2940

ccgaagacgu guaucuuggg auacaacaau guacgcuuug augacgaagu aacucguaac     3000

guguucuauc ggaacuucua ugacccuuac gccuggaguu ggcagcacga caauucucga     3060

ugggaucuuc uggaugugau gagagccugc uacgcucuca ggccagaggg cauuaacugg     3120

ccugagaacg acgaugggcu uccgucauuu cgccuugagc aucuuaccaa agcgaauggc     3180

aucgaacacu ccaaugcaca cgacgcuaug gcugaugucu acgcgacuau ugcaauggcg     3240

aaauugguca agacaagaca gccgagguug uuugacuacc uguuuacgca ucgaaacaaa     3300

cauaagcuca uggcacugau agaugucccu caaaugaagc cccucguuca cgucagcggu     3360

auguucggag cguggagagg aaacacgucc uggguugcuc cucuggcaug gcauccagag     3420

aacagaaacg cgguaaucau ggucgaucuc gcaggagaca uuagcccacu ucucgaauug     3480

gauuccgaca ccuugcgaga acgccuuuac acagcgaaaa cugauuuggg ugauaacgcu     3540

gccguaccug ucaaauuggu acauaucaac aaauguccgg ugcucgcgca agccaacacc     3600

cugagaccug aagacgcgga uagacugggg auaaaccggc agcacuguuu ggauaaucuc     3660

aagauacuca gggagaaucc gcaggugcgc gaaaaagucg uagccaucuu ugcggaggca     3720

gaaccauuua cgccgucuga caauguagau gcucaguuau acaauggcuu cuuuagugac     3780

gcugaccgag cugcgaugaa gauugugcua gagacugaac cgagaaaccu cucugcauug     3840

gacaucacuu uugucgauaa gcgaauagag aaguugcugu ucaacuaucg ugcccgaaac     3900

uuuccaggga cacucgauua cgcagagcaa caaagguggc uugagcaucg caggcaggug     3960

uuuaccccag aguucuugca ggguuacgcu gaggagauuc aaauguuggc acagcaguau     4020

gcagaugaua aggagaaagu cgcguugcug aaggcucugu ggcaauacgc cgaagagaua     4080

guguga                                                                4086


<210>  43
<211>  1361
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-ExoI protein 
       construct

<400>  43

Met Gly Ser Cys Arg Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser Ile Val 
            500                 505                 510         


Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp 
        515                 520                 525             


His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala 
    530                 535                 540                 


Val Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn 
545                 550                 555                 560 


Arg Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val 
                565                 570                 575     


Gly Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly 
            580                 585                 590         


Phe Ala Asp Ala Glu Gly Ser Phe Ile Leu Asp Ile Arg Asn Arg Asn 
        595                 600                 605             


Asn Glu Ser Asn Arg Tyr Arg Thr Ser Leu Arg Phe Gln Ile Thr Leu 
    610                 615                 620                 


His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys 
625                 630                 635                 640 


Val Gly Lys Ile Thr Asn Ser Ser Asp Arg Ala Val Met Leu Arg Val 
                645                 650                 655     


Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr 
            660                 665                 670         


Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala 
        675                 680                 685             


Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys 
    690                 695                 700                 


Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp Glu 
705                 710                 715                 720 


Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile 
                725                 730                 735     


Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ala Gly 
            740                 745                 750         


Glu Gly Tyr Phe Gly Val Asn Leu Lys Lys Val Lys Gly Thr Ala Lys 
        755                 760                 765             


Val Tyr Val Gly Leu Arg Phe Ser Ile Ser Gln His Ile Arg Asp Lys 
    770                 775                 780                 


Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Ser Ile Trp 
785                 790                 795                 800 


Glu Lys Asn Lys Ser Glu Phe Arg Trp Leu Glu Phe Val Val Thr Lys 
                805                 810                 815     


Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr 
            820                 825                 830         


Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala 
        835                 840                 845             


Lys Leu Ile Lys Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu 
    850                 855                 860                 


Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Gly Gly Gly Gly 
865                 870                 875                 880 


Ser Gly Gly Gly Gly Ser Ala Ser Asn Asp Gly Lys Gln Gln Ser Thr 
                885                 890                 895     


Phe Leu Phe His Asp Tyr Glu Thr Phe Gly Thr His Pro Ala Leu Asp 
            900                 905                 910         


Arg Pro Ala Gln Phe Ala Ala Ile Arg Thr Asp Asn Glu Phe Asn Val 
        915                 920                 925             


Ile Gly Glu Pro Glu Val Phe Tyr Cys Lys Pro Ala Asp Asp Tyr Leu 
    930                 935                 940                 


Pro Gln Pro Gly Ala Val Leu Ile Thr Gly Ile Thr Pro Gln Glu Ala 
945                 950                 955                 960 


Arg Ala Lys Gly Glu Asn Glu Ala Ala Phe Ala Ala Arg Ile His Ser 
                965                 970                 975     


Leu Phe Thr Val Pro Lys Thr Cys Ile Leu Gly Tyr Asn Asn Val Arg 
            980                 985                 990         


Phe Asp Asp Glu Val Thr Arg Asn  Val Phe Tyr Arg Asn  Phe Tyr Asp 
        995                 1000                 1005             


Pro Tyr  Ala Trp Ser Trp Gln  His Asp Asn Ser Arg  Trp Asp Leu 
    1010                 1015                 1020             


Leu Asp  Val Met Arg Ala Cys  Tyr Ala Leu Arg Pro  Glu Gly Ile 
    1025                 1030                 1035             


Asn Trp  Pro Glu Asn Asp Asp  Gly Leu Pro Ser Phe  Arg Leu Glu 
    1040                 1045                 1050             


His Leu  Thr Lys Ala Asn Gly  Ile Glu His Ser Asn  Ala His Asp 
    1055                 1060                 1065             


Ala Met  Ala Asp Val Tyr Ala  Thr Ile Ala Met Ala  Lys Leu Val 
    1070                 1075                 1080             


Lys Thr  Arg Gln Pro Arg Leu  Phe Asp Tyr Leu Phe  Thr His Arg 
    1085                 1090                 1095             


Asn Lys  His Lys Leu Met Ala  Leu Ile Asp Val Pro  Gln Met Lys 
    1100                 1105                 1110             


Pro Leu  Val His Val Ser Gly  Met Phe Gly Ala Trp  Arg Gly Asn 
    1115                 1120                 1125             


Thr Ser  Trp Val Ala Pro Leu  Ala Trp His Pro Glu  Asn Arg Asn 
    1130                 1135                 1140             


Ala Val  Ile Met Val Asp Leu  Ala Gly Asp Ile Ser  Pro Leu Leu 
    1145                 1150                 1155             


Glu Leu  Asp Ser Asp Thr Leu  Arg Glu Arg Leu Tyr  Thr Ala Lys 
    1160                 1165                 1170             


Thr Asp  Leu Gly Asp Asn Ala  Ala Val Pro Val Lys  Leu Val His 
    1175                 1180                 1185             


Ile Asn  Lys Cys Pro Val Leu  Ala Gln Ala Asn Thr  Leu Arg Pro 
    1190                 1195                 1200             


Glu Asp  Ala Asp Arg Leu Gly  Ile Asn Arg Gln His  Cys Leu Asp 
    1205                 1210                 1215             


Asn Leu  Lys Ile Leu Arg Glu  Asn Pro Gln Val Arg  Glu Lys Val 
    1220                 1225                 1230             


Val Ala  Ile Phe Ala Glu Ala  Glu Pro Phe Thr Pro  Ser Asp Asn 
    1235                 1240                 1245             


Val Asp  Ala Gln Leu Tyr Asn  Gly Phe Phe Ser Asp  Ala Asp Arg 
    1250                 1255                 1260             


Ala Ala  Met Lys Ile Val Leu  Glu Thr Glu Pro Arg  Asn Leu Ser 
    1265                 1270                 1275             


Ala Leu  Asp Ile Thr Phe Val  Asp Lys Arg Ile Glu  Lys Leu Leu 
    1280                 1285                 1290             


Phe Asn  Tyr Arg Ala Arg Asn  Phe Pro Gly Thr Leu  Asp Tyr Ala 
    1295                 1300                 1305             


Glu Gln  Gln Arg Trp Leu Glu  His Arg Arg Gln Val  Phe Thr Pro 
    1310                 1315                 1320             


Glu Phe  Leu Gln Gly Tyr Ala  Glu Glu Ile Gln Met  Leu Ala Gln 
    1325                 1330                 1335             


Gln Tyr  Ala Asp Asp Lys Glu  Lys Val Ala Leu Leu  Lys Ala Leu 
    1340                 1345                 1350             


Trp Gln  Tyr Ala Glu Glu Ile  Val 
    1355                 1360     


<210>  44
<211>  3324
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-ExoX DNA construct

<400>  44
atgggatcct gcaggccacc taagaagaaa cgcaaagtcg tggatctacg cacgctcggc       60

tacagtcagc agcagcaaga gaagatcaaa ccgaaggtgc gttcgacagt ggcgcagcac      120

cacgaggcac tggtgggcca tgggtttaca cacgcgcaca tcgttgcgct cagccaacac      180

ccggcagcgt tagggaccgt cgctgtcacg tatcagcaca taatcacggc gttgccagag      240

gcgacacacg aagacatcgt tggcgtcggc aaacagtggt ccggcgcacg cgccctggag      300

gccttgctca cggatgcggg ggagttgaga ggtccgccgt tacagttgga cacaggccaa      360

cttgtgaaga ttgcaaaacg tggcggcgtg accgcaatgg aggcagtgca tgcatcgcgc      420

aatgcactga cgggtgcccc cctgaacctg accccggacc aagtggtggc tatcgccagc      480

aacattggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag      540

gaccatggcc tgaccccgga ccaagtggtg gctatcgcca gcaacattgg cggcaagcaa      600

gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg      660

gaccaagtgg tggctatcgc cagcaacatt ggcggcaagc aagcgctcga aacggtgcag      720

cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc      780

gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg      840

tgccaggacc atggcctgac tccggaccaa gtggtggcta tcgccagcca cgatggcggc      900

aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg      960

actccggacc aagtggtggc tatcgccagc cacgatggcg gcaagcaagc gctcgaaacg     1020

gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg     1080

gctatcgcca gcaacattgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg     1140

gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc cagcaacaat     1200

ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat     1260

ggcctgaccc cggaccaagt ggtggctatc gccagcaacg gtggcggcaa gcaagcgctc     1320

gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa     1380

gtggtggcta tcgccagcaa caatggcggc aagcaagcgc tcgaaacggt gcagcggctg     1440

ttgccggtgc tgtgccagga ccatggcctg accccggacc aagtggtggc tatcgccagc     1500

aacattggcg gcaagcaagc gctcgaaagc attgtggccc agctgagccg gcctgatccg     1560

gcgttggccg cgttgaccaa cgaccacctc gtcgccttgg cctgcctcgg cggacgtcct     1620

gccatggatg cagtgaaaaa gggattgccg cacgcgccgg aattgatcag aagagtcaat     1680

cgccgtattg gcgaacgcac gtcccatcgc gttgcgatat ctagagtggg aggaagctct     1740

cgcagagagt ccatcaaccc atggattctg actggtttcg ctgatgccga aggatcattc     1800

atactagaca tccgcaaccg aaacaacgaa agcaacagat accgaacttc gctgagattc     1860

cagatcaccc tgcacaacaa ggacaaatcg attctggaga atatccagtc cacttggaag     1920

gtcggcaaga tcacaaacag cagtgacaga gccgtcatgc tgagggtcac ccgtttcgaa     1980

gatttgaaag tgattatcga ccacttcgag aaatatccgc tgattaccca gaaattgggc     2040

gattacaagt tgtttaaaca ggcattcagc gtcatggaga ataaagaaca tcttaaggag     2100

aatgggatta aggagctcgt acgaatcaaa gctaagatga attggggtct caatgacgaa     2160

ttgaaaaaag catttccaga gaacattagc aaagagcgcc cccttatcaa taagaacatt     2220

ccgaatttca aatggctggc tggattcaca gctggtgaag gctacttcgg cgtgaatcta     2280

aaaaaggtaa agggcaccgc aaaggtatac gtgggactga gattctcaat ctcacagcac     2340

atcagagaca agaacctgat gaattcattg ataacatacc taggctgtgg ttccatctgg     2400

gagaagaaca agtctgagtt cagatggctc gagttcgtcg taaccaaatt cagcgatatc     2460

aacgacaaga tcattccggt attccaggaa aatactctga ttggcgtcaa actcgaggac     2520

tttgaagatt ggtgcaaggt tgccaaattg atcaaagaga agaaacacct gaccgaatcc     2580

ggtttggatg agattaagaa aatcaagctg aacatgaaca aaggtcgtgg tggaggcggt     2640

agcggaggcg gagggtcggc tagcctgcgg atcatagaca cggaaacctg tggactccag     2700

ggtggtatcg tggagatagc cagtgtcgat gtaattgatg ggaaaatcgt aaacccgatg     2760

agccacctcg tccgaccgga taggccgata agtccgcagg ctatggctat acatagaatc     2820

acggaggcta tggtagctga caagccttgg atcgaggacg ttatcccaca ctactatggg     2880

agcgaatggt atgtagcgca caacgcatca tttgataggc gcgtgcttcc cgaaatgcct     2940

ggggagtgga tttgcacaat gaaactcgct cgccgactgt ggcctggtat caagtatagt     3000

aacatggcac tttacaagac ccgcaaactc aacgtacaga cacctccggg tctccaccac     3060

catagggcac tctatgattg ttacataacc gctgctctcc ttatagacat aatgaatacc     3120

tccgggtgga ccgccgaaca aatggcagac ataacgggaa gacctagttt gatgacaacc     3180

tttacatttg gaaagtatcg gggcaaggcg gtgagcgatg tggccgaaag agatcccggt     3240

tatcttagat ggctcttcaa taacttggat tctatgtcac ctgaacttcg ccttaccctg     3300

aagcattacc ttgagaatac ctga                                            3324


<210>  45
<211>  3324
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-ExoX RNA construct

<400>  45
augggauccu gcaggccacc uaagaagaaa cgcaaagucg uggaucuacg cacgcucggc       60

uacagucagc agcagcaaga gaagaucaaa ccgaaggugc guucgacagu ggcgcagcac      120

cacgaggcac uggugggcca uggguuuaca cacgcgcaca ucguugcgcu cagccaacac      180

ccggcagcgu uagggaccgu cgcugucacg uaucagcaca uaaucacggc guugccagag      240

gcgacacacg aagacaucgu uggcgucggc aaacaguggu ccggcgcacg cgcccuggag      300

gccuugcuca cggaugcggg ggaguugaga gguccgccgu uacaguugga cacaggccaa      360

cuugugaaga uugcaaaacg uggcggcgug accgcaaugg aggcagugca ugcaucgcgc      420

aaugcacuga cgggugcccc ccugaaccug accccggacc aagugguggc uaucgccagc      480

aacauuggcg gcaagcaagc gcucgaaacg gugcagcggc uguugccggu gcugugccag      540

gaccauggcc ugaccccgga ccaaguggug gcuaucgcca gcaacauugg cggcaagcaa      600

gcgcucgaaa cggugcagcg gcuguugccg gugcugugcc aggaccaugg ccugaccccg      660

gaccaagugg uggcuaucgc cagcaacauu ggcggcaagc aagcgcucga aacggugcag      720

cggcuguugc cggugcugug ccaggaccau ggccugaccc cggaccaagu gguggcuauc      780

gccagcaacg guggcggcaa gcaagcgcuc gaaacggugc agcggcuguu gccggugcug      840

ugccaggacc auggccugac uccggaccaa gugguggcua ucgccagcca cgauggcggc      900

aagcaagcgc ucgaaacggu gcagcggcug uugccggugc ugugccagga ccauggccug      960

acuccggacc aagugguggc uaucgccagc cacgauggcg gcaagcaagc gcucgaaacg     1020

gugcagcggc uguugccggu gcugugccag gaccauggcc ugaccccgga ccaaguggug     1080

gcuaucgcca gcaacauugg cggcaagcaa gcgcucgaaa cggugcagcg gcuguugccg     1140

gugcugugcc aggaccaugg ccugaccccg gaccaagugg uggcuaucgc cagcaacaau     1200

ggcggcaagc aagcgcucga aacggugcag cggcuguugc cggugcugug ccaggaccau     1260

ggccugaccc cggaccaagu gguggcuauc gccagcaacg guggcggcaa gcaagcgcuc     1320

gaaacggugc agcggcuguu gccggugcug ugccaggacc auggccugac cccggaccaa     1380

gugguggcua ucgccagcaa caauggcggc aagcaagcgc ucgaaacggu gcagcggcug     1440

uugccggugc ugugccagga ccauggccug accccggacc aagugguggc uaucgccagc     1500

aacauuggcg gcaagcaagc gcucgaaagc auuguggccc agcugagccg gccugauccg     1560

gcguuggccg cguugaccaa cgaccaccuc gucgccuugg ccugccucgg cggacguccu     1620

gccauggaug cagugaaaaa gggauugccg cacgcgccgg aauugaucag aagagucaau     1680

cgccguauug gcgaacgcac gucccaucgc guugcgauau cuagaguggg aggaagcucu     1740

cgcagagagu ccaucaaccc auggauucug acugguuucg cugaugccga aggaucauuc     1800

auacuagaca uccgcaaccg aaacaacgaa agcaacagau accgaacuuc gcugagauuc     1860

cagaucaccc ugcacaacaa ggacaaaucg auucuggaga auauccaguc cacuuggaag     1920

gucggcaaga ucacaaacag cagugacaga gccgucaugc ugagggucac ccguuucgaa     1980

gauuugaaag ugauuaucga ccacuucgag aaauauccgc ugauuaccca gaaauugggc     2040

gauuacaagu uguuuaaaca ggcauucagc gucauggaga auaaagaaca ucuuaaggag     2100

aaugggauua aggagcucgu acgaaucaaa gcuaagauga auuggggucu caaugacgaa     2160

uugaaaaaag cauuuccaga gaacauuagc aaagagcgcc cccuuaucaa uaagaacauu     2220

ccgaauuuca aauggcuggc uggauucaca gcuggugaag gcuacuucgg cgugaaucua     2280

aaaaagguaa agggcaccgc aaagguauac gugggacuga gauucucaau cucacagcac     2340

aucagagaca agaaccugau gaauucauug auaacauacc uaggcugugg uuccaucugg     2400

gagaagaaca agucugaguu cagauggcuc gaguucgucg uaaccaaauu cagcgauauc     2460

aacgacaaga ucauuccggu auuccaggaa aauacucuga uuggcgucaa acucgaggac     2520

uuugaagauu ggugcaaggu ugccaaauug aucaaagaga agaaacaccu gaccgaaucc     2580

gguuuggaug agauuaagaa aaucaagcug aacaugaaca aaggucgugg uggaggcggu     2640

agcggaggcg gagggucggc uagccugcgg aucauagaca cggaaaccug uggacuccag     2700

ggugguaucg uggagauagc cagugucgau guaauugaug ggaaaaucgu aaacccgaug     2760

agccaccucg uccgaccgga uaggccgaua aguccgcagg cuauggcuau acauagaauc     2820

acggaggcua ugguagcuga caagccuugg aucgaggacg uuaucccaca cuacuauggg     2880

agcgaauggu auguagcgca caacgcauca uuugauaggc gcgugcuucc cgaaaugccu     2940

ggggagugga uuugcacaau gaaacucgcu cgccgacugu ggccugguau caaguauagu     3000

aacauggcac uuuacaagac ccgcaaacuc aacguacaga caccuccggg ucuccaccac     3060

cauagggcac ucuaugauug uuacauaacc gcugcucucc uuauagacau aaugaauacc     3120

uccgggugga ccgccgaaca aauggcagac auaacgggaa gaccuaguuu gaugacaacc     3180

uuuacauuug gaaaguaucg gggcaaggcg gugagcgaug uggccgaaag agaucccggu     3240

uaucuuagau ggcucuucaa uaacuuggau ucuaugucac cugaacuucg ccuuacccug     3300

aagcauuacc uugagaauac cuga                                            3324


<210>  46
<211>  1107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-ExoX protein 
       construct

<400>  46

Met Gly Ser Cys Arg Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser Ile Val 
            500                 505                 510         


Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp 
        515                 520                 525             


His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala 
    530                 535                 540                 


Val Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn 
545                 550                 555                 560 


Arg Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val 
                565                 570                 575     


Gly Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly 
            580                 585                 590         


Phe Ala Asp Ala Glu Gly Ser Phe Ile Leu Asp Ile Arg Asn Arg Asn 
        595                 600                 605             


Asn Glu Ser Asn Arg Tyr Arg Thr Ser Leu Arg Phe Gln Ile Thr Leu 
    610                 615                 620                 


His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys 
625                 630                 635                 640 


Val Gly Lys Ile Thr Asn Ser Ser Asp Arg Ala Val Met Leu Arg Val 
                645                 650                 655     


Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr 
            660                 665                 670         


Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala 
        675                 680                 685             


Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys 
    690                 695                 700                 


Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp Glu 
705                 710                 715                 720 


Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile 
                725                 730                 735     


Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ala Gly 
            740                 745                 750         


Glu Gly Tyr Phe Gly Val Asn Leu Lys Lys Val Lys Gly Thr Ala Lys 
        755                 760                 765             


Val Tyr Val Gly Leu Arg Phe Ser Ile Ser Gln His Ile Arg Asp Lys 
    770                 775                 780                 


Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Ser Ile Trp 
785                 790                 795                 800 


Glu Lys Asn Lys Ser Glu Phe Arg Trp Leu Glu Phe Val Val Thr Lys 
                805                 810                 815     


Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr 
            820                 825                 830         


Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala 
        835                 840                 845             


Lys Leu Ile Lys Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu 
    850                 855                 860                 


Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Gly Gly Gly Gly 
865                 870                 875                 880 


Ser Gly Gly Gly Gly Ser Ala Ser Leu Arg Ile Ile Asp Thr Glu Thr 
                885                 890                 895     


Cys Gly Leu Gln Gly Gly Ile Val Glu Ile Ala Ser Val Asp Val Ile 
            900                 905                 910         


Asp Gly Lys Ile Val Asn Pro Met Ser His Leu Val Arg Pro Asp Arg 
        915                 920                 925             


Pro Ile Ser Pro Gln Ala Met Ala Ile His Arg Ile Thr Glu Ala Met 
    930                 935                 940                 


Val Ala Asp Lys Pro Trp Ile Glu Asp Val Ile Pro His Tyr Tyr Gly 
945                 950                 955                 960 


Ser Glu Trp Tyr Val Ala His Asn Ala Ser Phe Asp Arg Arg Val Leu 
                965                 970                 975     


Pro Glu Met Pro Gly Glu Trp Ile Cys Thr Met Lys Leu Ala Arg Arg 
            980                 985                 990         


Leu Trp Pro Gly Ile Lys Tyr Ser  Asn Met Ala Leu Tyr  Lys Thr Arg 
        995                 1000                 1005             


Lys Leu  Asn Val Gln Thr Pro  Pro Gly Leu His His  His Arg Ala 
    1010                 1015                 1020             


Leu Tyr  Asp Cys Tyr Ile Thr  Ala Ala Leu Leu Ile  Asp Ile Met 
    1025                 1030                 1035             


Asn Thr  Ser Gly Trp Thr Ala  Glu Gln Met Ala Asp  Ile Thr Gly 
    1040                 1045                 1050             


Arg Pro  Ser Leu Met Thr Thr  Phe Thr Phe Gly Lys  Tyr Arg Gly 
    1055                 1060                 1065             


Lys Ala  Val Ser Asp Val Ala  Glu Arg Asp Pro Gly  Tyr Leu Arg 
    1070                 1075                 1080             


Trp Leu  Phe Asn Asn Leu Asp  Ser Met Ser Pro Glu  Leu Arg Leu 
    1085                 1090                 1095             


Thr Leu  Lys His Tyr Leu Glu  Asn Thr 
    1100                 1105         


<210>  47
<211>  3537
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-T5FEN DNA construct

<400>  47
atgggatcct gcaggccacc taagaagaaa cgcaaagtcg tggatctacg cacgctcggc       60

tacagtcagc agcagcaaga gaagatcaaa ccgaaggtgc gttcgacagt ggcgcagcac      120

cacgaggcac tggtgggcca tgggtttaca cacgcgcaca tcgttgcgct cagccaacac      180

ccggcagcgt tagggaccgt cgctgtcacg tatcagcaca taatcacggc gttgccagag      240

gcgacacacg aagacatcgt tggcgtcggc aaacagtggt ccggcgcacg cgccctggag      300

gccttgctca cggatgcggg ggagttgaga ggtccgccgt tacagttgga cacaggccaa      360

cttgtgaaga ttgcaaaacg tggcggcgtg accgcaatgg aggcagtgca tgcatcgcgc      420

aatgcactga cgggtgcccc cctgaacctg accccggacc aagtggtggc tatcgccagc      480

aacattggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag      540

gaccatggcc tgaccccgga ccaagtggtg gctatcgcca gcaacattgg cggcaagcaa      600

gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg      660

gaccaagtgg tggctatcgc cagcaacatt ggcggcaagc aagcgctcga aacggtgcag      720

cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc      780

gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg      840

tgccaggacc atggcctgac tccggaccaa gtggtggcta tcgccagcca cgatggcggc      900

aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg      960

actccggacc aagtggtggc tatcgccagc cacgatggcg gcaagcaagc gctcgaaacg     1020

gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg     1080

gctatcgcca gcaacattgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg     1140

gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc cagcaacaat     1200

ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat     1260

ggcctgaccc cggaccaagt ggtggctatc gccagcaacg gtggcggcaa gcaagcgctc     1320

gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa     1380

gtggtggcta tcgccagcaa caatggcggc aagcaagcgc tcgaaacggt gcagcggctg     1440

ttgccggtgc tgtgccagga ccatggcctg accccggacc aagtggtggc tatcgccagc     1500

aacattggcg gcaagcaagc gctcgaaagc attgtggccc agctgagccg gcctgatccg     1560

gcgttggccg cgttgaccaa cgaccacctc gtcgccttgg cctgcctcgg cggacgtcct     1620

gccatggatg cagtgaaaaa gggattgccg cacgcgccgg aattgatcag aagagtcaat     1680

cgccgtattg gcgaacgcac gtcccatcgc gttgcgatat ctagagtggg aggaagctct     1740

cgcagagagt ccatcaaccc atggattctg actggtttcg ctgatgccga aggatcattc     1800

atactagaca tccgcaaccg aaacaacgaa agcaacagat accgaacttc gctgagattc     1860

cagatcaccc tgcacaacaa ggacaaatcg attctggaga atatccagtc cacttggaag     1920

gtcggcaaga tcacaaacag cagtgacaga gccgtcatgc tgagggtcac ccgtttcgaa     1980

gatttgaaag tgattatcga ccacttcgag aaatatccgc tgattaccca gaaattgggc     2040

gattacaagt tgtttaaaca ggcattcagc gtcatggaga ataaagaaca tcttaaggag     2100

aatgggatta aggagctcgt acgaatcaaa gctaagatga attggggtct caatgacgaa     2160

ttgaaaaaag catttccaga gaacattagc aaagagcgcc cccttatcaa taagaacatt     2220

ccgaatttca aatggctggc tggattcaca gctggtgaag gctacttcgg cgtgaatcta     2280

aaaaaggtaa agggcaccgc aaaggtatac gtgggactga gattctcaat ctcacagcac     2340

atcagagaca agaacctgat gaattcattg ataacatacc taggctgtgg ttccatctgg     2400

gagaagaaca agtctgagtt cagatggctc gagttcgtcg taaccaaatt cagcgatatc     2460

aacgacaaga tcattccggt attccaggaa aatactctga ttggcgtcaa actcgaggac     2520

tttgaagatt ggtgcaaggt tgccaaattg atcaaagaga agaaacacct gaccgaatcc     2580

ggtttggatg agattaagaa aatcaagctg aacatgaaca aaggtcgtgg tggaggcggt     2640

agcggaggcg gagggtcggc tagcagtaag agctggggga agttcataga ggaggaggaa     2700

gctgaaatgg caagcagacg caatttgatg attgtggacg gcactaatct tggtttcagg     2760

ttcaaacata acaatagtaa gaagccattt gccagcagct atgtttctac gatacaatca     2820

ctggctaaaa gttacagtgc gaggactaca atagtccttg gcgacaaagg caaatcagtg     2880

ttcaggctcg aacatctccc tgaatacaag ggcaaccgcg atgagaagta tgcacagcga     2940

acggaagaag agaaagccct ggacgagcaa ttctttgaat atctcaaaga tgcctttgaa     3000

ctgtgcaaaa ctacatttcc aacctttacc atccggggag ttgaggcaga tgacatggca     3060

gcctacattg taaaactgat agggcacctc tacgaccatg tttggctcat atccactgac     3120

ggagactggg acactttgtt gaccgataaa gtgtcaaggt tctcattcac tactcgtcgg     3180

gaatatcatt tgcgcgatat gtatgaacac cataacgtcg atgacgtgga acaattcatc     3240

tctcttaaag caatcatggg cgatctgggg gacaacatta ggggggttga ggggatcgga     3300

gcgaaaaggg gatacaatat catacgggaa tttggcaacg tccttgacat catcgaccag     3360

ctccccttgc ccggaaagca gaagtacatt caaaacctca acgcttcaga agaactgctc     3420

tttaggaact tgatactggt agacctccca acttactgcg tggatgcaat cgcggcggta     3480

ggacaggacg tgcttgacaa attcacgaag gacatactgg agattgccga acagtga        3537


<210>  48
<211>  3537
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-T5FEN RNA construct

<400>  48
augggauccu gcaggccacc uaagaagaaa cgcaaagucg uggaucuacg cacgcucggc       60

uacagucagc agcagcaaga gaagaucaaa ccgaaggugc guucgacagu ggcgcagcac      120

cacgaggcac uggugggcca uggguuuaca cacgcgcaca ucguugcgcu cagccaacac      180

ccggcagcgu uagggaccgu cgcugucacg uaucagcaca uaaucacggc guugccagag      240

gcgacacacg aagacaucgu uggcgucggc aaacaguggu ccggcgcacg cgcccuggag      300

gccuugcuca cggaugcggg ggaguugaga gguccgccgu uacaguugga cacaggccaa      360

cuugugaaga uugcaaaacg uggcggcgug accgcaaugg aggcagugca ugcaucgcgc      420

aaugcacuga cgggugcccc ccugaaccug accccggacc aagugguggc uaucgccagc      480

aacauuggcg gcaagcaagc gcucgaaacg gugcagcggc uguugccggu gcugugccag      540

gaccauggcc ugaccccgga ccaaguggug gcuaucgcca gcaacauugg cggcaagcaa      600

gcgcucgaaa cggugcagcg gcuguugccg gugcugugcc aggaccaugg ccugaccccg      660

gaccaagugg uggcuaucgc cagcaacauu ggcggcaagc aagcgcucga aacggugcag      720

cggcuguugc cggugcugug ccaggaccau ggccugaccc cggaccaagu gguggcuauc      780

gccagcaacg guggcggcaa gcaagcgcuc gaaacggugc agcggcuguu gccggugcug      840

ugccaggacc auggccugac uccggaccaa gugguggcua ucgccagcca cgauggcggc      900

aagcaagcgc ucgaaacggu gcagcggcug uugccggugc ugugccagga ccauggccug      960

acuccggacc aagugguggc uaucgccagc cacgauggcg gcaagcaagc gcucgaaacg     1020

gugcagcggc uguugccggu gcugugccag gaccauggcc ugaccccgga ccaaguggug     1080

gcuaucgcca gcaacauugg cggcaagcaa gcgcucgaaa cggugcagcg gcuguugccg     1140

gugcugugcc aggaccaugg ccugaccccg gaccaagugg uggcuaucgc cagcaacaau     1200

ggcggcaagc aagcgcucga aacggugcag cggcuguugc cggugcugug ccaggaccau     1260

ggccugaccc cggaccaagu gguggcuauc gccagcaacg guggcggcaa gcaagcgcuc     1320

gaaacggugc agcggcuguu gccggugcug ugccaggacc auggccugac cccggaccaa     1380

gugguggcua ucgccagcaa caauggcggc aagcaagcgc ucgaaacggu gcagcggcug     1440

uugccggugc ugugccagga ccauggccug accccggacc aagugguggc uaucgccagc     1500

aacauuggcg gcaagcaagc gcucgaaagc auuguggccc agcugagccg gccugauccg     1560

gcguuggccg cguugaccaa cgaccaccuc gucgccuugg ccugccucgg cggacguccu     1620

gccauggaug cagugaaaaa gggauugccg cacgcgccgg aauugaucag aagagucaau     1680

cgccguauug gcgaacgcac gucccaucgc guugcgauau cuagaguggg aggaagcucu     1740

cgcagagagu ccaucaaccc auggauucug acugguuucg cugaugccga aggaucauuc     1800

auacuagaca uccgcaaccg aaacaacgaa agcaacagau accgaacuuc gcugagauuc     1860

cagaucaccc ugcacaacaa ggacaaaucg auucuggaga auauccaguc cacuuggaag     1920

gucggcaaga ucacaaacag cagugacaga gccgucaugc ugagggucac ccguuucgaa     1980

gauuugaaag ugauuaucga ccacuucgag aaauauccgc ugauuaccca gaaauugggc     2040

gauuacaagu uguuuaaaca ggcauucagc gucauggaga auaaagaaca ucuuaaggag     2100

aaugggauua aggagcucgu acgaaucaaa gcuaagauga auuggggucu caaugacgaa     2160

uugaaaaaag cauuuccaga gaacauuagc aaagagcgcc cccuuaucaa uaagaacauu     2220

ccgaauuuca aauggcuggc uggauucaca gcuggugaag gcuacuucgg cgugaaucua     2280

aaaaagguaa agggcaccgc aaagguauac gugggacuga gauucucaau cucacagcac     2340

aucagagaca agaaccugau gaauucauug auaacauacc uaggcugugg uuccaucugg     2400

gagaagaaca agucugaguu cagauggcuc gaguucgucg uaaccaaauu cagcgauauc     2460

aacgacaaga ucauuccggu auuccaggaa aauacucuga uuggcgucaa acucgaggac     2520

uuugaagauu ggugcaaggu ugccaaauug aucaaagaga agaaacaccu gaccgaaucc     2580

gguuuggaug agauuaagaa aaucaagcug aacaugaaca aaggucgugg uggaggcggu     2640

agcggaggcg gagggucggc uagcaguaag agcuggggga aguucauaga ggaggaggaa     2700

gcugaaaugg caagcagacg caauuugaug auuguggacg gcacuaaucu ugguuucagg     2760

uucaaacaua acaauaguaa gaagccauuu gccagcagcu auguuucuac gauacaauca     2820

cuggcuaaaa guuacagugc gaggacuaca auaguccuug gcgacaaagg caaaucagug     2880

uucaggcucg aacaucuccc ugaauacaag ggcaaccgcg augagaagua ugcacagcga     2940

acggaagaag agaaagcccu ggacgagcaa uucuuugaau aucucaaaga ugccuuugaa     3000

cugugcaaaa cuacauuucc aaccuuuacc auccggggag uugaggcaga ugacauggca     3060

gccuacauug uaaaacugau agggcaccuc uacgaccaug uuuggcucau auccacugac     3120

ggagacuggg acacuuuguu gaccgauaaa gugucaaggu ucucauucac uacucgucgg     3180

gaauaucauu ugcgcgauau guaugaacac cauaacgucg augacgugga acaauucauc     3240

ucucuuaaag caaucauggg cgaucugggg gacaacauua gggggguuga ggggaucgga     3300

gcgaaaaggg gauacaauau cauacgggaa uuuggcaacg uccuugacau caucgaccag     3360

cuccccuugc ccggaaagca gaaguacauu caaaaccuca acgcuucaga agaacugcuc     3420

uuuaggaacu ugauacuggu agaccuccca acuuacugcg uggaugcaau cgcggcggua     3480

ggacaggacg ugcuugacaa auucacgaag gacauacugg agauugccga acaguga        3537


<210>  49
<211>  1178
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-T5FEN protein 
       construct

<400>  49

Met Gly Ser Cys Arg Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser Ile Val 
            500                 505                 510         


Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp 
        515                 520                 525             


His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala 
    530                 535                 540                 


Val Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn 
545                 550                 555                 560 


Arg Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val 
                565                 570                 575     


Gly Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly 
            580                 585                 590         


Phe Ala Asp Ala Glu Gly Ser Phe Ile Leu Asp Ile Arg Asn Arg Asn 
        595                 600                 605             


Asn Glu Ser Asn Arg Tyr Arg Thr Ser Leu Arg Phe Gln Ile Thr Leu 
    610                 615                 620                 


His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys 
625                 630                 635                 640 


Val Gly Lys Ile Thr Asn Ser Ser Asp Arg Ala Val Met Leu Arg Val 
                645                 650                 655     


Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr 
            660                 665                 670         


Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala 
        675                 680                 685             


Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys 
    690                 695                 700                 


Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp Glu 
705                 710                 715                 720 


Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile 
                725                 730                 735     


Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ala Gly 
            740                 745                 750         


Glu Gly Tyr Phe Gly Val Asn Leu Lys Lys Val Lys Gly Thr Ala Lys 
        755                 760                 765             


Val Tyr Val Gly Leu Arg Phe Ser Ile Ser Gln His Ile Arg Asp Lys 
    770                 775                 780                 


Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Ser Ile Trp 
785                 790                 795                 800 


Glu Lys Asn Lys Ser Glu Phe Arg Trp Leu Glu Phe Val Val Thr Lys 
                805                 810                 815     


Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr 
            820                 825                 830         


Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala 
        835                 840                 845             


Lys Leu Ile Lys Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu 
    850                 855                 860                 


Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Gly Gly Gly Gly 
865                 870                 875                 880 


Ser Gly Gly Gly Gly Ser Ala Ser Ser Lys Ser Trp Gly Lys Phe Ile 
                885                 890                 895     


Glu Glu Glu Glu Ala Glu Met Ala Ser Arg Arg Asn Leu Met Ile Val 
            900                 905                 910         


Asp Gly Thr Asn Leu Gly Phe Arg Phe Lys His Asn Asn Ser Lys Lys 
        915                 920                 925             


Pro Phe Ala Ser Ser Tyr Val Ser Thr Ile Gln Ser Leu Ala Lys Ser 
    930                 935                 940                 


Tyr Ser Ala Arg Thr Thr Ile Val Leu Gly Asp Lys Gly Lys Ser Val 
945                 950                 955                 960 


Phe Arg Leu Glu His Leu Pro Glu Tyr Lys Gly Asn Arg Asp Glu Lys 
                965                 970                 975     


Tyr Ala Gln Arg Thr Glu Glu Glu Lys Ala Leu Asp Glu Gln Phe Phe 
            980                 985                 990         


Glu Tyr Leu Lys Asp Ala Phe Glu  Leu Cys Lys Thr Thr  Phe Pro Thr 
        995                 1000                 1005             


Phe Thr  Ile Arg Gly Val Glu  Ala Asp Asp Met Ala  Ala Tyr Ile 
    1010                 1015                 1020             


Val Lys  Leu Ile Gly His Leu  Tyr Asp His Val Trp  Leu Ile Ser 
    1025                 1030                 1035             


Thr Asp  Gly Asp Trp Asp Thr  Leu Leu Thr Asp Lys  Val Ser Arg 
    1040                 1045                 1050             


Phe Ser  Phe Thr Thr Arg Arg  Glu Tyr His Leu Arg  Asp Met Tyr 
    1055                 1060                 1065             


Glu His  His Asn Val Asp Asp  Val Glu Gln Phe Ile  Ser Leu Lys 
    1070                 1075                 1080             


Ala Ile  Met Gly Asp Leu Gly  Asp Asn Ile Arg Gly  Val Glu Gly 
    1085                 1090                 1095             


Ile Gly  Ala Lys Arg Gly Tyr  Asn Ile Ile Arg Glu  Phe Gly Asn 
    1100                 1105                 1110             


Val Leu  Asp Ile Ile Asp Gln  Leu Pro Leu Pro Gly  Lys Gln Lys 
    1115                 1120                 1125             


Tyr Ile  Gln Asn Leu Asn Ala  Ser Glu Glu Leu Leu  Phe Arg Asn 
    1130                 1135                 1140             


Leu Ile  Leu Val Asp Leu Pro  Thr Tyr Cys Val Asp  Ala Ile Ala 
    1145                 1150                 1155             


Ala Val  Gly Gln Asp Val Leu  Asp Lys Phe Thr Lys  Asp Ile Leu 
    1160                 1165                 1170             


Glu Ile  Ala Glu Gln 
    1175             


<210>  50
<211>  3342
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-lambdaExo DNA 
       construct

<400>  50
atgggatcct gcaggccacc taagaagaaa cgcaaagtcg tggatctacg cacgctcggc       60

tacagtcagc agcagcaaga gaagatcaaa ccgaaggtgc gttcgacagt ggcgcagcac      120

cacgaggcac tggtgggcca tgggtttaca cacgcgcaca tcgttgcgct cagccaacac      180

ccggcagcgt tagggaccgt cgctgtcacg tatcagcaca taatcacggc gttgccagag      240

gcgacacacg aagacatcgt tggcgtcggc aaacagtggt ccggcgcacg cgccctggag      300

gccttgctca cggatgcggg ggagttgaga ggtccgccgt tacagttgga cacaggccaa      360

cttgtgaaga ttgcaaaacg tggcggcgtg accgcaatgg aggcagtgca tgcatcgcgc      420

aatgcactga cgggtgcccc cctgaacctg accccggacc aagtggtggc tatcgccagc      480

aacattggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag      540

gaccatggcc tgaccccgga ccaagtggtg gctatcgcca gcaacattgg cggcaagcaa      600

gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg      660

gaccaagtgg tggctatcgc cagcaacatt ggcggcaagc aagcgctcga aacggtgcag      720

cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc      780

gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg      840

tgccaggacc atggcctgac tccggaccaa gtggtggcta tcgccagcca cgatggcggc      900

aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg      960

actccggacc aagtggtggc tatcgccagc cacgatggcg gcaagcaagc gctcgaaacg     1020

gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg     1080

gctatcgcca gcaacattgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg     1140

gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc cagcaacaat     1200

ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat     1260

ggcctgaccc cggaccaagt ggtggctatc gccagcaacg gtggcggcaa gcaagcgctc     1320

gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa     1380

gtggtggcta tcgccagcaa caatggcggc aagcaagcgc tcgaaacggt gcagcggctg     1440

ttgccggtgc tgtgccagga ccatggcctg accccggacc aagtggtggc tatcgccagc     1500

aacattggcg gcaagcaagc gctcgaaagc attgtggccc agctgagccg gcctgatccg     1560

gcgttggccg cgttgaccaa cgaccacctc gtcgccttgg cctgcctcgg cggacgtcct     1620

gccatggatg cagtgaaaaa gggattgccg cacgcgccgg aattgatcag aagagtcaat     1680

cgccgtattg gcgaacgcac gtcccatcgc gttgcgatat ctagagtggg aggaagctct     1740

cgcagagagt ccatcaaccc atggattctg actggtttcg ctgatgccga aggatcattc     1800

atactagaca tccgcaaccg aaacaacgaa agcaacagat accgaacttc gctgagattc     1860

cagatcaccc tgcacaacaa ggacaaatcg attctggaga atatccagtc cacttggaag     1920

gtcggcaaga tcacaaacag cagtgacaga gccgtcatgc tgagggtcac ccgtttcgaa     1980

gatttgaaag tgattatcga ccacttcgag aaatatccgc tgattaccca gaaattgggc     2040

gattacaagt tgtttaaaca ggcattcagc gtcatggaga ataaagaaca tcttaaggag     2100

aatgggatta aggagctcgt acgaatcaaa gctaagatga attggggtct caatgacgaa     2160

ttgaaaaaag catttccaga gaacattagc aaagagcgcc cccttatcaa taagaacatt     2220

ccgaatttca aatggctggc tggattcaca gctggtgaag gctacttcgg cgtgaatcta     2280

aaaaaggtaa agggcaccgc aaaggtatac gtgggactga gattctcaat ctcacagcac     2340

atcagagaca agaacctgat gaattcattg ataacatacc taggctgtgg ttccatctgg     2400

gagaagaaca agtctgagtt cagatggctc gagttcgtcg taaccaaatt cagcgatatc     2460

aacgacaaga tcattccggt attccaggaa aatactctga ttggcgtcaa actcgaggac     2520

tttgaagatt ggtgcaaggt tgccaaattg atcaaagaga agaaacacct gaccgaatcc     2580

ggtttggatg agattaagaa aatcaagctg aacatgaaca aaggtcgtgg tggaggcggt     2640

agcggaggcg gagggtcggc tagcactccc gacatcatac tgcaacgaac cggaattgac     2700

gttcgagcgg tcgagcaagg ggatgatgcc tggcataagt tgagacttgg tgtgataact     2760

gcatcagagg tacataatgt cattgcgaag ccccggagcg ggaaaaagtg gcccgacatg     2820

aagatgtcat actttcatac actgctggcc gaggtctgca cgggtgttgc tcccgaggtt     2880

aatgccaaag cacttgcatg gggtaaacag tacgaaaacg acgctcggac cctctttgag     2940

ttcacatctg gtgtgaatgt gacagagagc ccaatcattt accgagacga gagcatgaga     3000

acggcttgca gtcctgatgg cctgtgcagc gacggtaacg gactggagct aaagtgcccg     3060

tttactagcc gagactttat gaagtttagg ctcggtgggt ttgaggccat caagagtgct     3120

tacatggcgc aagtacagta ttccatgtgg gtcactcgca aaaacgcttg gtactttgct     3180

aactatgatc cccgaatgaa gagggagggc ctgcattatg tggtcataga acgggacgaa     3240

aagtatatgg cgagcttcga cgaaattgta ccggagttca tagagaagat ggatgaagca     3300

ctcgccgaga ttgggttcgt ctttggggaa caatggaggt ga                        3342


<210>  51
<211>  3342
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-lambdaExo RNA 
       construct

<400>  51
augggauccu gcaggccacc uaagaagaaa cgcaaagucg uggaucuacg cacgcucggc       60

uacagucagc agcagcaaga gaagaucaaa ccgaaggugc guucgacagu ggcgcagcac      120

cacgaggcac uggugggcca uggguuuaca cacgcgcaca ucguugcgcu cagccaacac      180

ccggcagcgu uagggaccgu cgcugucacg uaucagcaca uaaucacggc guugccagag      240

gcgacacacg aagacaucgu uggcgucggc aaacaguggu ccggcgcacg cgcccuggag      300

gccuugcuca cggaugcggg ggaguugaga gguccgccgu uacaguugga cacaggccaa      360

cuugugaaga uugcaaaacg uggcggcgug accgcaaugg aggcagugca ugcaucgcgc      420

aaugcacuga cgggugcccc ccugaaccug accccggacc aagugguggc uaucgccagc      480

aacauuggcg gcaagcaagc gcucgaaacg gugcagcggc uguugccggu gcugugccag      540

gaccauggcc ugaccccgga ccaaguggug gcuaucgcca gcaacauugg cggcaagcaa      600

gcgcucgaaa cggugcagcg gcuguugccg gugcugugcc aggaccaugg ccugaccccg      660

gaccaagugg uggcuaucgc cagcaacauu ggcggcaagc aagcgcucga aacggugcag      720

cggcuguugc cggugcugug ccaggaccau ggccugaccc cggaccaagu gguggcuauc      780

gccagcaacg guggcggcaa gcaagcgcuc gaaacggugc agcggcuguu gccggugcug      840

ugccaggacc auggccugac uccggaccaa gugguggcua ucgccagcca cgauggcggc      900

aagcaagcgc ucgaaacggu gcagcggcug uugccggugc ugugccagga ccauggccug      960

acuccggacc aagugguggc uaucgccagc cacgauggcg gcaagcaagc gcucgaaacg     1020

gugcagcggc uguugccggu gcugugccag gaccauggcc ugaccccgga ccaaguggug     1080

gcuaucgcca gcaacauugg cggcaagcaa gcgcucgaaa cggugcagcg gcuguugccg     1140

gugcugugcc aggaccaugg ccugaccccg gaccaagugg uggcuaucgc cagcaacaau     1200

ggcggcaagc aagcgcucga aacggugcag cggcuguugc cggugcugug ccaggaccau     1260

ggccugaccc cggaccaagu gguggcuauc gccagcaacg guggcggcaa gcaagcgcuc     1320

gaaacggugc agcggcuguu gccggugcug ugccaggacc auggccugac cccggaccaa     1380

gugguggcua ucgccagcaa caauggcggc aagcaagcgc ucgaaacggu gcagcggcug     1440

uugccggugc ugugccagga ccauggccug accccggacc aagugguggc uaucgccagc     1500

aacauuggcg gcaagcaagc gcucgaaagc auuguggccc agcugagccg gccugauccg     1560

gcguuggccg cguugaccaa cgaccaccuc gucgccuugg ccugccucgg cggacguccu     1620

gccauggaug cagugaaaaa gggauugccg cacgcgccgg aauugaucag aagagucaau     1680

cgccguauug gcgaacgcac gucccaucgc guugcgauau cuagaguggg aggaagcucu     1740

cgcagagagu ccaucaaccc auggauucug acugguuucg cugaugccga aggaucauuc     1800

auacuagaca uccgcaaccg aaacaacgaa agcaacagau accgaacuuc gcugagauuc     1860

cagaucaccc ugcacaacaa ggacaaaucg auucuggaga auauccaguc cacuuggaag     1920

gucggcaaga ucacaaacag cagugacaga gccgucaugc ugagggucac ccguuucgaa     1980

gauuugaaag ugauuaucga ccacuucgag aaauauccgc ugauuaccca gaaauugggc     2040

gauuacaagu uguuuaaaca ggcauucagc gucauggaga auaaagaaca ucuuaaggag     2100

aaugggauua aggagcucgu acgaaucaaa gcuaagauga auuggggucu caaugacgaa     2160

uugaaaaaag cauuuccaga gaacauuagc aaagagcgcc cccuuaucaa uaagaacauu     2220

ccgaauuuca aauggcuggc uggauucaca gcuggugaag gcuacuucgg cgugaaucua     2280

aaaaagguaa agggcaccgc aaagguauac gugggacuga gauucucaau cucacagcac     2340

aucagagaca agaaccugau gaauucauug auaacauacc uaggcugugg uuccaucugg     2400

gagaagaaca agucugaguu cagauggcuc gaguucgucg uaaccaaauu cagcgauauc     2460

aacgacaaga ucauuccggu auuccaggaa aauacucuga uuggcgucaa acucgaggac     2520

uuugaagauu ggugcaaggu ugccaaauug aucaaagaga agaaacaccu gaccgaaucc     2580

gguuuggaug agauuaagaa aaucaagcug aacaugaaca aaggucgugg uggaggcggu     2640

agcggaggcg gagggucggc uagcacuccc gacaucauac ugcaacgaac cggaauugac     2700

guucgagcgg ucgagcaagg ggaugaugcc uggcauaagu ugagacuugg ugugauaacu     2760

gcaucagagg uacauaaugu cauugcgaag ccccggagcg ggaaaaagug gcccgacaug     2820

aagaugucau acuuucauac acugcuggcc gaggucugca cggguguugc ucccgagguu     2880

aaugccaaag cacuugcaug ggguaaacag uacgaaaacg acgcucggac ccucuuugag     2940

uucacaucug gugugaaugu gacagagagc ccaaucauuu accgagacga gagcaugaga     3000

acggcuugca guccugaugg ccugugcagc gacgguaacg gacuggagcu aaagugcccg     3060

uuuacuagcc gagacuuuau gaaguuuagg cucggugggu uugaggccau caagagugcu     3120

uacauggcgc aaguacagua uuccaugugg gucacucgca aaaacgcuug guacuuugcu     3180

aacuaugauc cccgaaugaa gagggagggc cugcauuaug uggucauaga acgggacgaa     3240

aaguauaugg cgagcuucga cgaaauugua ccggaguuca uagagaagau ggaugaagca     3300

cucgccgaga uuggguucgu cuuuggggaa caauggaggu ga                        3342


<210>  52
<211>  1113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-lambdaExo protein 
       construct

<400>  52

Met Gly Ser Cys Arg Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser Ile Val 
            500                 505                 510         


Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp 
        515                 520                 525             


His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala 
    530                 535                 540                 


Val Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn 
545                 550                 555                 560 


Arg Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val 
                565                 570                 575     


Gly Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly 
            580                 585                 590         


Phe Ala Asp Ala Glu Gly Ser Phe Ile Leu Asp Ile Arg Asn Arg Asn 
        595                 600                 605             


Asn Glu Ser Asn Arg Tyr Arg Thr Ser Leu Arg Phe Gln Ile Thr Leu 
    610                 615                 620                 


His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys 
625                 630                 635                 640 


Val Gly Lys Ile Thr Asn Ser Ser Asp Arg Ala Val Met Leu Arg Val 
                645                 650                 655     


Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr 
            660                 665                 670         


Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala 
        675                 680                 685             


Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys 
    690                 695                 700                 


Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp Glu 
705                 710                 715                 720 


Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile 
                725                 730                 735     


Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ala Gly 
            740                 745                 750         


Glu Gly Tyr Phe Gly Val Asn Leu Lys Lys Val Lys Gly Thr Ala Lys 
        755                 760                 765             


Val Tyr Val Gly Leu Arg Phe Ser Ile Ser Gln His Ile Arg Asp Lys 
    770                 775                 780                 


Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Ser Ile Trp 
785                 790                 795                 800 


Glu Lys Asn Lys Ser Glu Phe Arg Trp Leu Glu Phe Val Val Thr Lys 
                805                 810                 815     


Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr 
            820                 825                 830         


Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala 
        835                 840                 845             


Lys Leu Ile Lys Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu 
    850                 855                 860                 


Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Gly Gly Gly Gly 
865                 870                 875                 880 


Ser Gly Gly Gly Gly Ser Ala Ser Thr Pro Asp Ile Ile Leu Gln Arg 
                885                 890                 895     


Thr Gly Ile Asp Val Arg Ala Val Glu Gln Gly Asp Asp Ala Trp His 
            900                 905                 910         


Lys Leu Arg Leu Gly Val Ile Thr Ala Ser Glu Val His Asn Val Ile 
        915                 920                 925             


Ala Lys Pro Arg Ser Gly Lys Lys Trp Pro Asp Met Lys Met Ser Tyr 
    930                 935                 940                 


Phe His Thr Leu Leu Ala Glu Val Cys Thr Gly Val Ala Pro Glu Val 
945                 950                 955                 960 


Asn Ala Lys Ala Leu Ala Trp Gly Lys Gln Tyr Glu Asn Asp Ala Arg 
                965                 970                 975     


Thr Leu Phe Glu Phe Thr Ser Gly Val Asn Val Thr Glu Ser Pro Ile 
            980                 985                 990         


Ile Tyr Arg Asp Glu Ser Met Arg  Thr Ala Cys Ser Pro  Asp Gly Leu 
        995                 1000                 1005             


Cys Ser  Asp Gly Asn Gly Leu  Glu Leu Lys Cys Pro  Phe Thr Ser 
    1010                 1015                 1020             


Arg Asp  Phe Met Lys Phe Arg  Leu Gly Gly Phe Glu  Ala Ile Lys 
    1025                 1030                 1035             


Ser Ala  Tyr Met Ala Gln Val  Gln Tyr Ser Met Trp  Val Thr Arg 
    1040                 1045                 1050             


Lys Asn  Ala Trp Tyr Phe Ala  Asn Tyr Asp Pro Arg  Met Lys Arg 
    1055                 1060                 1065             


Glu Gly  Leu His Tyr Val Val  Ile Glu Arg Asp Glu  Lys Tyr Met 
    1070                 1075                 1080             


Ala Ser  Phe Asp Glu Ile Val  Pro Glu Phe Ile Glu  Lys Met Asp 
    1085                 1090                 1095             


Glu Ala  Leu Ala Glu Ile Gly  Phe Val Phe Gly Glu  Gln Trp Arg 
    1100                 1105                 1110             


<210>  53
<211>  4398
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-RecJ DNA construct

<400>  53
atgggatcct gcaggccacc taagaagaaa cgcaaagtcg tggatctacg cacgctcggc       60

tacagtcagc agcagcaaga gaagatcaaa ccgaaggtgc gttcgacagt ggcgcagcac      120

cacgaggcac tggtgggcca tgggtttaca cacgcgcaca tcgttgcgct cagccaacac      180

ccggcagcgt tagggaccgt cgctgtcacg tatcagcaca taatcacggc gttgccagag      240

gcgacacacg aagacatcgt tggcgtcggc aaacagtggt ccggcgcacg cgccctggag      300

gccttgctca cggatgcggg ggagttgaga ggtccgccgt tacagttgga cacaggccaa      360

cttgtgaaga ttgcaaaacg tggcggcgtg accgcaatgg aggcagtgca tgcatcgcgc      420

aatgcactga cgggtgcccc cctgaacctg accccggacc aagtggtggc tatcgccagc      480

aacattggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag      540

gaccatggcc tgaccccgga ccaagtggtg gctatcgcca gcaacattgg cggcaagcaa      600

gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg      660

gaccaagtgg tggctatcgc cagcaacatt ggcggcaagc aagcgctcga aacggtgcag      720

cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc      780

gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg      840

tgccaggacc atggcctgac tccggaccaa gtggtggcta tcgccagcca cgatggcggc      900

aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg      960

actccggacc aagtggtggc tatcgccagc cacgatggcg gcaagcaagc gctcgaaacg     1020

gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg     1080

gctatcgcca gcaacattgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg     1140

gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc cagcaacaat     1200

ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat     1260

ggcctgaccc cggaccaagt ggtggctatc gccagcaacg gtggcggcaa gcaagcgctc     1320

gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa     1380

gtggtggcta tcgccagcaa caatggcggc aagcaagcgc tcgaaacggt gcagcggctg     1440

ttgccggtgc tgtgccagga ccatggcctg accccggacc aagtggtggc tatcgccagc     1500

aacattggcg gcaagcaagc gctcgaaagc attgtggccc agctgagccg gcctgatccg     1560

gcgttggccg cgttgaccaa cgaccacctc gtcgccttgg cctgcctcgg cggacgtcct     1620

gccatggatg cagtgaaaaa gggattgccg cacgcgccgg aattgatcag aagagtcaat     1680

cgccgtattg gcgaacgcac gtcccatcgc gttgcgatat ctagagtggg aggaagctct     1740

cgcagagagt ccatcaaccc atggattctg actggtttcg ctgatgccga aggatcattc     1800

atactagaca tccgcaaccg aaacaacgaa agcaacagat accgaacttc gctgagattc     1860

cagatcaccc tgcacaacaa ggacaaatcg attctggaga atatccagtc cacttggaag     1920

gtcggcaaga tcacaaacag cagtgacaga gccgtcatgc tgagggtcac ccgtttcgaa     1980

gatttgaaag tgattatcga ccacttcgag aaatatccgc tgattaccca gaaattgggc     2040

gattacaagt tgtttaaaca ggcattcagc gtcatggaga ataaagaaca tcttaaggag     2100

aatgggatta aggagctcgt acgaatcaaa gctaagatga attggggtct caatgacgaa     2160

ttgaaaaaag catttccaga gaacattagc aaagagcgcc cccttatcaa taagaacatt     2220

ccgaatttca aatggctggc tggattcaca gctggtgaag gctacttcgg cgtgaatcta     2280

aaaaaggtaa agggcaccgc aaaggtatac gtgggactga gattctcaat ctcacagcac     2340

atcagagaca agaacctgat gaattcattg ataacatacc taggctgtgg ttccatctgg     2400

gagaagaaca agtctgagtt cagatggctc gagttcgtcg taaccaaatt cagcgatatc     2460

aacgacaaga tcattccggt attccaggaa aatactctga ttggcgtcaa actcgaggac     2520

tttgaagatt ggtgcaaggt tgccaaattg atcaaagaga agaaacacct gaccgaatcc     2580

ggtttggatg agattaagaa aatcaagctg aacatgaaca aaggtcgtgg tggaggcggt     2640

agcggaggcg gagggtcggc tagcaagcaa caaatccatt tgagacgaag ggaggttgac     2700

gagactgctg atctcccggc tgcggagttg cctccgctat tgcgtcgcct gtacgcctct     2760

cggggtgtac gctccgctca agaactggaa cgatcagtaa agggtatgct gccctggcaa     2820

cagctttccg gggtggagaa ggccgtggag atactataca atgcgttccg agaagggacg     2880

agaatcatag tagttgggga ctttgatgcg gatggcgcga cttccactgc cctgtctgtg     2940

ctggcgatgc ggagtcttgg gtgttccaac atagactacc tcgttccaaa tcggtttgaa     3000

gatggatacg gcctttcacc cgaagttgtg gaccaagccc acgcgagggg agcccaactt     3060

atagtgacag tggataacgg aattagctca catgcaggag tagagcacgc aaggtcactg     3120

ggtatccccg tgattgtcac cgaccatcac ctcccaggag acactttgcc tgctgctgag     3180

gccatcatta accctaatct tagggactgc aactttccta gtaagagtct ggctggggtt     3240

ggagtcgcat tctaccttat gctggccctc cgcacttttc tccgagatca aggctggttt     3300

gatgaacgaa acattgctat cccaaatctt gcagaactcc ttgaccttgt ggctttggga     3360

acagtcgccg atgtcgtgcc gctggacgct aacaatcgga tattgacttg gcaggggatg     3420

tcacgcatcc gtgcgggcaa gtgcaggcca ggtatcaagg cattgttgga agtcgccaac     3480

cgggacgcac aaaaacttgc cgcaagcgac ctcggttttg cacttggtcc tcgccttaac     3540

gcagcgggta gattggatga tatgtccgtg ggcgtggcgc tcttgttgtg cgacaacata     3600

ggtgaagcga gagttcttgc aaatgagttg gacgcgctca atcagactag gaaagagata     3660

gaacaaggaa tgcagatcga ggcactgacc ctttgtgaaa agctggagcg atcacgggat     3720

accttgcctg gaggtctcgc aatgtaccat cctgagtggc atcagggcgt cgtaggtatc     3780

ttggcgtcta ggatcaaaga gaggttccat cgcccagtga ttgcatttgc gccagcaggt     3840

gatggcactc tgaaaggatc tggccgctcc atccaaggtc tccacatgag agatgcactt     3900

gagaggttgg acacacttta cccaggcatg atgttgaaat tcggtgggca cgctatggct     3960

gctggtttga gcttggaaga agataagttc aaactgttcc aacagaggtt cggcgaactg     4020

gtaactgagt ggcttgatcc ttcattgctc caaggtgagg tggtatcaga tgggccactc     4080

agcccagccg aaatgactat ggaagtagcc cagctcctca gagatgcagg tccttggggt     4140

caaatgttcc ccgagccctt gttcgacggg catttcaggt tgctccagca gaggttggta     4200

ggcgagcgcc acctcaaagt gatggtcgaa cctgtaggtg gaggtccgct tctggacgga     4260

attgctttca acgtagacac ggcgctgtgg cccgacaatg gcgttaggga ggtacaactt     4320

gcctacaaac tcgacatcaa cgagtttaga ggtaacagaa gcctccagat catcattgac     4380

aatatctggc ccatttga                                                   4398


<210>  54
<211>  4398
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-RecJ RNA construct

<400>  54
augggauccu gcaggccacc uaagaagaaa cgcaaagucg uggaucuacg cacgcucggc       60

uacagucagc agcagcaaga gaagaucaaa ccgaaggugc guucgacagu ggcgcagcac      120

cacgaggcac uggugggcca uggguuuaca cacgcgcaca ucguugcgcu cagccaacac      180

ccggcagcgu uagggaccgu cgcugucacg uaucagcaca uaaucacggc guugccagag      240

gcgacacacg aagacaucgu uggcgucggc aaacaguggu ccggcgcacg cgcccuggag      300

gccuugcuca cggaugcggg ggaguugaga gguccgccgu uacaguugga cacaggccaa      360

cuugugaaga uugcaaaacg uggcggcgug accgcaaugg aggcagugca ugcaucgcgc      420

aaugcacuga cgggugcccc ccugaaccug accccggacc aagugguggc uaucgccagc      480

aacauuggcg gcaagcaagc gcucgaaacg gugcagcggc uguugccggu gcugugccag      540

gaccauggcc ugaccccgga ccaaguggug gcuaucgcca gcaacauugg cggcaagcaa      600

gcgcucgaaa cggugcagcg gcuguugccg gugcugugcc aggaccaugg ccugaccccg      660

gaccaagugg uggcuaucgc cagcaacauu ggcggcaagc aagcgcucga aacggugcag      720

cggcuguugc cggugcugug ccaggaccau ggccugaccc cggaccaagu gguggcuauc      780

gccagcaacg guggcggcaa gcaagcgcuc gaaacggugc agcggcuguu gccggugcug      840

ugccaggacc auggccugac uccggaccaa gugguggcua ucgccagcca cgauggcggc      900

aagcaagcgc ucgaaacggu gcagcggcug uugccggugc ugugccagga ccauggccug      960

acuccggacc aagugguggc uaucgccagc cacgauggcg gcaagcaagc gcucgaaacg     1020

gugcagcggc uguugccggu gcugugccag gaccauggcc ugaccccgga ccaaguggug     1080

gcuaucgcca gcaacauugg cggcaagcaa gcgcucgaaa cggugcagcg gcuguugccg     1140

gugcugugcc aggaccaugg ccugaccccg gaccaagugg uggcuaucgc cagcaacaau     1200

ggcggcaagc aagcgcucga aacggugcag cggcuguugc cggugcugug ccaggaccau     1260

ggccugaccc cggaccaagu gguggcuauc gccagcaacg guggcggcaa gcaagcgcuc     1320

gaaacggugc agcggcuguu gccggugcug ugccaggacc auggccugac cccggaccaa     1380

gugguggcua ucgccagcaa caauggcggc aagcaagcgc ucgaaacggu gcagcggcug     1440

uugccggugc ugugccagga ccauggccug accccggacc aagugguggc uaucgccagc     1500

aacauuggcg gcaagcaagc gcucgaaagc auuguggccc agcugagccg gccugauccg     1560

gcguuggccg cguugaccaa cgaccaccuc gucgccuugg ccugccucgg cggacguccu     1620

gccauggaug cagugaaaaa gggauugccg cacgcgccgg aauugaucag aagagucaau     1680

cgccguauug gcgaacgcac gucccaucgc guugcgauau cuagaguggg aggaagcucu     1740

cgcagagagu ccaucaaccc auggauucug acugguuucg cugaugccga aggaucauuc     1800

auacuagaca uccgcaaccg aaacaacgaa agcaacagau accgaacuuc gcugagauuc     1860

cagaucaccc ugcacaacaa ggacaaaucg auucuggaga auauccaguc cacuuggaag     1920

gucggcaaga ucacaaacag cagugacaga gccgucaugc ugagggucac ccguuucgaa     1980

gauuugaaag ugauuaucga ccacuucgag aaauauccgc ugauuaccca gaaauugggc     2040

gauuacaagu uguuuaaaca ggcauucagc gucauggaga auaaagaaca ucuuaaggag     2100

aaugggauua aggagcucgu acgaaucaaa gcuaagauga auuggggucu caaugacgaa     2160

uugaaaaaag cauuuccaga gaacauuagc aaagagcgcc cccuuaucaa uaagaacauu     2220

ccgaauuuca aauggcuggc uggauucaca gcuggugaag gcuacuucgg cgugaaucua     2280

aaaaagguaa agggcaccgc aaagguauac gugggacuga gauucucaau cucacagcac     2340

aucagagaca agaaccugau gaauucauug auaacauacc uaggcugugg uuccaucugg     2400

gagaagaaca agucugaguu cagauggcuc gaguucgucg uaaccaaauu cagcgauauc     2460

aacgacaaga ucauuccggu auuccaggaa aauacucuga uuggcgucaa acucgaggac     2520

uuugaagauu ggugcaaggu ugccaaauug aucaaagaga agaaacaccu gaccgaaucc     2580

gguuuggaug agauuaagaa aaucaagcug aacaugaaca aaggucgugg uggaggcggu     2640

agcggaggcg gagggucggc uagcaagcaa caaauccauu ugagacgaag ggagguugac     2700

gagacugcug aucucccggc ugcggaguug ccuccgcuau ugcgucgccu guacgccucu     2760

cgggguguac gcuccgcuca agaacuggaa cgaucaguaa aggguaugcu gcccuggcaa     2820

cagcuuuccg ggguggagaa ggccguggag auacuauaca augcguuccg agaagggacg     2880

agaaucauag uaguugggga cuuugaugcg gauggcgcga cuuccacugc ccugucugug     2940

cuggcgaugc ggagucuugg guguuccaac auagacuacc ucguuccaaa ucgguuugaa     3000

gauggauacg gccuuucacc cgaaguugug gaccaagccc acgcgagggg agcccaacuu     3060

auagugacag uggauaacgg aauuagcuca caugcaggag uagagcacgc aaggucacug     3120

gguauccccg ugauugucac cgaccaucac cucccaggag acacuuugcc ugcugcugag     3180

gccaucauua acccuaaucu uagggacugc aacuuuccua guaagagucu ggcugggguu     3240

ggagucgcau ucuaccuuau gcuggcccuc cgcacuuuuc uccgagauca aggcugguuu     3300

gaugaacgaa acauugcuau cccaaaucuu gcagaacucc uugaccuugu ggcuuuggga     3360

acagucgccg augucgugcc gcuggacgcu aacaaucgga uauugacuug gcaggggaug     3420

ucacgcaucc gugcgggcaa gugcaggcca gguaucaagg cauuguugga agucgccaac     3480

cgggacgcac aaaaacuugc cgcaagcgac cucgguuuug cacuuggucc ucgccuuaac     3540

gcagcgggua gauuggauga uauguccgug ggcguggcgc ucuuguugug cgacaacaua     3600

ggugaagcga gaguucuugc aaaugaguug gacgcgcuca aucagacuag gaaagagaua     3660

gaacaaggaa ugcagaucga ggcacugacc cuuugugaaa agcuggagcg aucacgggau     3720

accuugccug gaggucucgc aauguaccau ccugaguggc aucagggcgu cguagguauc     3780

uuggcgucua ggaucaaaga gagguuccau cgcccaguga uugcauuugc gccagcaggu     3840

gauggcacuc ugaaaggauc uggccgcucc auccaagguc uccacaugag agaugcacuu     3900

gagagguugg acacacuuua cccaggcaug auguugaaau ucggugggca cgcuauggcu     3960

gcugguuuga gcuuggaaga agauaaguuc aaacuguucc aacagagguu cggcgaacug     4020

guaacugagu ggcuugaucc uucauugcuc caaggugagg ugguaucaga ugggccacuc     4080

agcccagccg aaaugacuau ggaaguagcc cagcuccuca gagaugcagg uccuuggggu     4140

caaauguucc ccgagcccuu guucgacggg cauuucaggu ugcuccagca gagguuggua     4200

ggcgagcgcc accucaaagu gauggucgaa ccuguaggug gagguccgcu ucuggacgga     4260

auugcuuuca acguagacac ggcgcugugg cccgacaaug gcguuaggga gguacaacuu     4320

gccuacaaac ucgacaucaa cgaguuuaga gguaacagaa gccuccagau caucauugac     4380

aauaucuggc ccauuuga                                                   4398


<210>  55
<211>  1465
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity TCRalpha megaTAL-RecJ protein 
       construct

<400>  55

Met Gly Ser Cys Arg Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser Ile Val 
            500                 505                 510         


Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp 
        515                 520                 525             


His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala 
    530                 535                 540                 


Val Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn 
545                 550                 555                 560 


Arg Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val 
                565                 570                 575     


Gly Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr Gly 
            580                 585                 590         


Phe Ala Asp Ala Glu Gly Ser Phe Ile Leu Asp Ile Arg Asn Arg Asn 
        595                 600                 605             


Asn Glu Ser Asn Arg Tyr Arg Thr Ser Leu Arg Phe Gln Ile Thr Leu 
    610                 615                 620                 


His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys 
625                 630                 635                 640 


Val Gly Lys Ile Thr Asn Ser Ser Asp Arg Ala Val Met Leu Arg Val 
                645                 650                 655     


Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr 
            660                 665                 670         


Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala 
        675                 680                 685             


Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys 
    690                 695                 700                 


Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp Glu 
705                 710                 715                 720 


Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile 
                725                 730                 735     


Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ala Gly 
            740                 745                 750         


Glu Gly Tyr Phe Gly Val Asn Leu Lys Lys Val Lys Gly Thr Ala Lys 
        755                 760                 765             


Val Tyr Val Gly Leu Arg Phe Ser Ile Ser Gln His Ile Arg Asp Lys 
    770                 775                 780                 


Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Ser Ile Trp 
785                 790                 795                 800 


Glu Lys Asn Lys Ser Glu Phe Arg Trp Leu Glu Phe Val Val Thr Lys 
                805                 810                 815     


Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr 
            820                 825                 830         


Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala 
        835                 840                 845             


Lys Leu Ile Lys Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu 
    850                 855                 860                 


Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Gly Gly Gly Gly 
865                 870                 875                 880 


Ser Gly Gly Gly Gly Ser Ala Ser Lys Gln Gln Ile His Leu Arg Arg 
                885                 890                 895     


Arg Glu Val Asp Glu Thr Ala Asp Leu Pro Ala Ala Glu Leu Pro Pro 
            900                 905                 910         


Leu Leu Arg Arg Leu Tyr Ala Ser Arg Gly Val Arg Ser Ala Gln Glu 
        915                 920                 925             


Leu Glu Arg Ser Val Lys Gly Met Leu Pro Trp Gln Gln Leu Ser Gly 
    930                 935                 940                 


Val Glu Lys Ala Val Glu Ile Leu Tyr Asn Ala Phe Arg Glu Gly Thr 
945                 950                 955                 960 


Arg Ile Ile Val Val Gly Asp Phe Asp Ala Asp Gly Ala Thr Ser Thr 
                965                 970                 975     


Ala Leu Ser Val Leu Ala Met Arg Ser Leu Gly Cys Ser Asn Ile Asp 
            980                 985                 990         


Tyr Leu Val Pro Asn Arg Phe Glu  Asp Gly Tyr Gly Leu  Ser Pro Glu 
        995                 1000                 1005             


Val Val  Asp Gln Ala His Ala  Arg Gly Ala Gln Leu  Ile Val Thr 
    1010                 1015                 1020             


Val Asp  Asn Gly Ile Ser Ser  His Ala Gly Val Glu  His Ala Arg 
    1025                 1030                 1035             


Ser Leu  Gly Ile Pro Val Ile  Val Thr Asp His His  Leu Pro Gly 
    1040                 1045                 1050             


Asp Thr  Leu Pro Ala Ala Glu  Ala Ile Ile Asn Pro  Asn Leu Arg 
    1055                 1060                 1065             


Asp Cys  Asn Phe Pro Ser Lys  Ser Leu Ala Gly Val  Gly Val Ala 
    1070                 1075                 1080             


Phe Tyr  Leu Met Leu Ala Leu  Arg Thr Phe Leu Arg  Asp Gln Gly 
    1085                 1090                 1095             


Trp Phe  Asp Glu Arg Asn Ile  Ala Ile Pro Asn Leu  Ala Glu Leu 
    1100                 1105                 1110             


Leu Asp  Leu Val Ala Leu Gly  Thr Val Ala Asp Val  Val Pro Leu 
    1115                 1120                 1125             


Asp Ala  Asn Asn Arg Ile Leu  Thr Trp Gln Gly Met  Ser Arg Ile 
    1130                 1135                 1140             


Arg Ala  Gly Lys Cys Arg Pro  Gly Ile Lys Ala Leu  Leu Glu Val 
    1145                 1150                 1155             


Ala Asn  Arg Asp Ala Gln Lys  Leu Ala Ala Ser Asp  Leu Gly Phe 
    1160                 1165                 1170             


Ala Leu  Gly Pro Arg Leu Asn  Ala Ala Gly Arg Leu  Asp Asp Met 
    1175                 1180                 1185             


Ser Val  Gly Val Ala Leu Leu  Leu Cys Asp Asn Ile  Gly Glu Ala 
    1190                 1195                 1200             


Arg Val  Leu Ala Asn Glu Leu  Asp Ala Leu Asn Gln  Thr Arg Lys 
    1205                 1210                 1215             


Glu Ile  Glu Gln Gly Met Gln  Ile Glu Ala Leu Thr  Leu Cys Glu 
    1220                 1225                 1230             


Lys Leu  Glu Arg Ser Arg Asp  Thr Leu Pro Gly Gly  Leu Ala Met 
    1235                 1240                 1245             


Tyr His  Pro Glu Trp His Gln  Gly Val Val Gly Ile  Leu Ala Ser 
    1250                 1255                 1260             


Arg Ile  Lys Glu Arg Phe His  Arg Pro Val Ile Ala  Phe Ala Pro 
    1265                 1270                 1275             


Ala Gly  Asp Gly Thr Leu Lys  Gly Ser Gly Arg Ser  Ile Gln Gly 
    1280                 1285                 1290             


Leu His  Met Arg Asp Ala Leu  Glu Arg Leu Asp Thr  Leu Tyr Pro 
    1295                 1300                 1305             


Gly Met  Met Leu Lys Phe Gly  Gly His Ala Met Ala  Ala Gly Leu 
    1310                 1315                 1320             


Ser Leu  Glu Glu Asp Lys Phe  Lys Leu Phe Gln Gln  Arg Phe Gly 
    1325                 1330                 1335             


Glu Leu  Val Thr Glu Trp Leu  Asp Pro Ser Leu Leu  Gln Gly Glu 
    1340                 1345                 1350             


Val Val  Ser Asp Gly Pro Leu  Ser Pro Ala Glu Met  Thr Met Glu 
    1355                 1360                 1365             


Val Ala  Gln Leu Leu Arg Asp  Ala Gly Pro Trp Gly  Gln Met Phe 
    1370                 1375                 1380             


Pro Glu  Pro Leu Phe Asp Gly  His Phe Arg Leu Leu  Gln Gln Arg 
    1385                 1390                 1395             


Leu Val  Gly Glu Arg His Leu  Lys Val Met Val Glu  Pro Val Gly 
    1400                 1405                 1410             


Gly Gly  Pro Leu Leu Asp Gly  Ile Ala Phe Asn Val  Asp Thr Ala 
    1415                 1420                 1425             


Leu Trp  Pro Asp Asn Gly Val  Arg Glu Val Gln Leu  Ala Tyr Lys 
    1430                 1435                 1440             


Leu Asp  Ile Asn Glu Phe Arg  Gly Asn Arg Ser Leu  Gln Ile Ile 
    1445                 1450                 1455             


Ile Asp  Asn Ile Trp Pro Ile  
    1460                 1465 


<210>  56
<211>  2733
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - high activity Cb1-b megaTAL DNA construct

<400>  56
atgggatcct gcaggccacc taagaagaaa cgcaaagtcg tggatctacg cacgctcggc       60

tacagtcagc agcagcaaga gaagatcaaa ccgaaggtgc gttcgacagt ggcgcagcac      120

cacgaggcac tggtgggcca tgggtttaca cacgcgcaca tcgttgcgct cagccaacac      180

ccggcagcgt tagggaccgt cgctgtcacg tatcagcaca taatcacggc gttgccagag      240

gcgacacacg aagacatcgt tggcgtcggc aaacagtggt ccggcgcacg cgccctggag      300

gccttgctca cggatgcggg ggagttgaga ggtccgccgt tacagttgga cacaggccaa      360

cttgtgaaga ttgcaaaacg tggcggcgtg accgcaatgg aggcagtgca tgcatcgcgc      420

aatgcactga cgggtgcccc cctgaactta acacccgatc aagtggtagc gatagcgtca      480

aacggcgggg gtaaacaggc tttggagacg gtacagcggt tattgccggt actctgccag      540

gaccacggat tgacaccgga ccaagtggtg gcgattgcgt ccaacaacgg aggcaagcag      600

gcactagaga ctgtccaacg gcttcttccc gttctttgtc aggatcatgg gctaacccct      660

gatcaggtag tcgctatagc ttcaaatgga gggggcaagc aagcactgga gactgttcaa      720

cgactcctgc cagtgctctg ccaagaccac ggacttacac cagatcaagt ggttgctatt      780

gcctccaatg gtggcgggaa acaagcgttg gaaactgtgc agagactgtt acctgtcttg      840

tgtcaagacc acggcctcac gccagatcag gtggtagcca tagcgtcgaa cattggtggt      900

aagcaagccc ttgaaacggt ccagcgtctt ctgccggtgt tgtgccagga ccacggacta      960

acgccggatc aggtcgtagc cattgcttca aatgggggcg gcaaacaggc gctagagaca     1020

gtccagcgcc tcttgcctgt gttatgccag gatcacggct taaccccaga ccaagttgtg     1080

gctattgcat ctaacaatgg tggcaaacaa gccttggaga cagtgcaacg attactgcct     1140

gtcttatgtc aggatcatgg cctgacgccc gatcaggtag tggcaatcgc atctaacatt     1200

ggaggtaagc aagcactgga gactgtccag agattgttac ccgtactatg tcaagatcat     1260

ggtttgacgc ctgatcaggt tgttgcgata gccagcaata acggagggaa acaggctctt     1320

gaaaccgtac agcgacttct cccagtcttg tgccaagatc acgggcttac tcctgatcaa     1380

gtcgtagcta tcgccagcaa taacggtgga aaacaggccc tggaaaccgt acaacgtctc     1440

ctcccagtac tttgtcaaga ccacgggttg actccggatc aagtcgtcgc gatcgcgagc     1500

aatggtgggg ggaagcaggc gctggaaact gttcagagac tgctgcctgt actttgtcag     1560

gaccatggtc tgacacctga ccaagttgtg gcgatagcca gtaatatcgg gggaaaacag     1620

gcactagaga gcattgtggc ccagctgagc cggcctgatc cggcgttggc cgcgttgacc     1680

aacgaccacc tcgtcgcctt ggcctgcctc ggcggacgtc ctgccatgga tgcagtgaaa     1740

aagggattgc cgcacgcgcc ggaattgatc agaagagtca atcgccgtat tggcgaacgc     1800

acgtcccatc gcgttgcgat atctagagtg ggaggaagct cgcgcagaga gtccatcaac     1860

ccatggattc tgactggttt cgctgatgcc gaaggatgct tccgactaga catccgcaac     1920

gcaaacgatt taagagccgg atacagaact agactggcct tcgaaatcgt actgcacaac     1980

aaggacaaat cgattctgga gaatatccag tcgacttgga aggtcggcac aatctacaac     2040

gcgggcgaca acgcagtcag actgcaagtc acacgtttcg aagatttgaa agtgattatc     2100

gaccacttcg agaaatatcc gctgataaca cagaaattgg gcgattacaa gttgtttaaa     2160

caggcattca gcgtcatgga gaacaaagaa catcttaagg agaatgggat taaggagctc     2220

gtacgaatca aagctaagat gaattggggt ctcaatgacg aattgaaaaa agcatttcca     2280

gagaacatta gcaaagagcg ctcccttatc aataagaaca ttccgaatct caaatggctg     2340

gctggattca catctggtga cggctcgttc gtggtggaac taaagaagag aagaagcccc     2400

gtcaaggtag gagtgcggct gcgattcagc atcacccagc acatcagaga caagaacctg     2460

atgaattcat tgataacata cctaggctgt ggtcgtatcg ttgagaataa caaatctgag     2520

cacagttggc tcgaattcat tgtaacaaaa ttcagcgata tcaacgacaa gatcattccg     2580

gtattccagg aaaatactct gattggcgtc aaactcgagg actttgaaga ttggtgcaag     2640

gttgccaaat tgatcgaaga gaagaaacac ctgaccgaat ccggtttgga tgagattaag     2700

aaaatcaagc tgaacatgaa caaaggtcgt tga                                  2733


<210>  57
<211>  2733
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - high activity Cb1-b megaTAL RNA construct

<400>  57
augggauccu gcaggccacc uaagaagaaa cgcaaagucg uggaucuacg cacgcucggc       60

uacagucagc agcagcaaga gaagaucaaa ccgaaggugc guucgacagu ggcgcagcac      120

cacgaggcac uggugggcca uggguuuaca cacgcgcaca ucguugcgcu cagccaacac      180

ccggcagcgu uagggaccgu cgcugucacg uaucagcaca uaaucacggc guugccagag      240

gcgacacacg aagacaucgu uggcgucggc aaacaguggu ccggcgcacg cgcccuggag      300

gccuugcuca cggaugcggg ggaguugaga gguccgccgu uacaguugga cacaggccaa      360

cuugugaaga uugcaaaacg uggcggcgug accgcaaugg aggcagugca ugcaucgcgc      420

aaugcacuga cgggugcccc ccugaacuua acacccgauc aagugguagc gauagcguca      480

aacggcgggg guaaacaggc uuuggagacg guacagcggu uauugccggu acucugccag      540

gaccacggau ugacaccgga ccaaguggug gcgauugcgu ccaacaacgg aggcaagcag      600

gcacuagaga cuguccaacg gcuucuuccc guucuuuguc aggaucaugg gcuaaccccu      660

gaucagguag ucgcuauagc uucaaaugga gggggcaagc aagcacugga gacuguucaa      720

cgacuccugc cagugcucug ccaagaccac ggacuuacac cagaucaagu gguugcuauu      780

gccuccaaug guggcgggaa acaagcguug gaaacugugc agagacuguu accugucuug      840

ugucaagacc acggccucac gccagaucag gugguagcca uagcgucgaa cauugguggu      900

aagcaagccc uugaaacggu ccagcgucuu cugccggugu ugugccagga ccacggacua      960

acgccggauc aggucguagc cauugcuuca aaugggggcg gcaaacaggc gcuagagaca     1020

guccagcgcc ucuugccugu guuaugccag gaucacggcu uaaccccaga ccaaguugug     1080

gcuauugcau cuaacaaugg uggcaaacaa gccuuggaga cagugcaacg auuacugccu     1140

gucuuauguc aggaucaugg ccugacgccc gaucagguag uggcaaucgc aucuaacauu     1200

ggagguaagc aagcacugga gacuguccag agauuguuac ccguacuaug ucaagaucau     1260

gguuugacgc cugaucaggu uguugcgaua gccagcaaua acggagggaa acaggcucuu     1320

gaaaccguac agcgacuucu cccagucuug ugccaagauc acgggcuuac uccugaucaa     1380

gucguagcua ucgccagcaa uaacggugga aaacaggccc uggaaaccgu acaacgucuc     1440

cucccaguac uuugucaaga ccacggguug acuccggauc aagucgucgc gaucgcgagc     1500

aauggugggg ggaagcaggc gcuggaaacu guucagagac ugcugccugu acuuugucag     1560

gaccaugguc ugacaccuga ccaaguugug gcgauagcca guaauaucgg gggaaaacag     1620

gcacuagaga gcauuguggc ccagcugagc cggccugauc cggcguuggc cgcguugacc     1680

aacgaccacc ucgucgccuu ggccugccuc ggcggacguc cugccaugga ugcagugaaa     1740

aagggauugc cgcacgcgcc ggaauugauc agaagaguca aucgccguau uggcgaacgc     1800

acgucccauc gcguugcgau aucuagagug ggaggaagcu cgcgcagaga guccaucaac     1860

ccauggauuc ugacugguuu cgcugaugcc gaaggaugcu uccgacuaga cauccgcaac     1920

gcaaacgauu uaagagccgg auacagaacu agacuggccu ucgaaaucgu acugcacaac     1980

aaggacaaau cgauucugga gaauauccag ucgacuugga aggucggcac aaucuacaac     2040

gcgggcgaca acgcagucag acugcaaguc acacguuucg aagauuugaa agugauuauc     2100

gaccacuucg agaaauaucc gcugauaaca cagaaauugg gcgauuacaa guuguuuaaa     2160

caggcauuca gcgucaugga gaacaaagaa caucuuaagg agaaugggau uaaggagcuc     2220

guacgaauca aagcuaagau gaauuggggu cucaaugacg aauugaaaaa agcauuucca     2280

gagaacauua gcaaagagcg cucccuuauc aauaagaaca uuccgaaucu caaauggcug     2340

gcuggauuca caucugguga cggcucguuc gugguggaac uaaagaagag aagaagcccc     2400

gucaagguag gagugcggcu gcgauucagc aucacccagc acaucagaga caagaaccug     2460

augaauucau ugauaacaua ccuaggcugu ggucguaucg uugagaauaa caaaucugag     2520

cacaguuggc ucgaauucau uguaacaaaa uucagcgaua ucaacgacaa gaucauuccg     2580

guauuccagg aaaauacucu gauuggcguc aaacucgagg acuuugaaga uuggugcaag     2640

guugccaaau ugaucgaaga gaagaaacac cugaccgaau ccgguuugga ugagauuaag     2700

aaaaucaagc ugaacaugaa caaaggucgu uga                                  2733


<210>  58
<211>  910
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - high activity Cb1-b megaTAL protein construct

<400>  58

Met Gly Ser Cys Arg Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            500                 505                 510         


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
        515                 520                 525             


Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser 
    530                 535                 540                 


Ile Val Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr 
545                 550                 555                 560 


Asn Asp His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met 
                565                 570                 575     


Asp Ala Val Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg 
            580                 585                 590         


Val Asn Arg Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser 
        595                 600                 605             


Arg Val Gly Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu 
    610                 615                 620                 


Thr Gly Phe Ala Asp Ala Glu Gly Cys Phe Arg Leu Asp Ile Arg Asn 
625                 630                 635                 640 


Ala Asn Asp Leu Arg Ala Gly Tyr Arg Thr Arg Leu Ala Phe Glu Ile 
                645                 650                 655     


Val Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr 
            660                 665                 670         


Trp Lys Val Gly Thr Ile Tyr Asn Ala Gly Asp Asn Ala Val Arg Leu 
        675                 680                 685             


Gln Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu 
    690                 695                 700                 


Lys Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys 
705                 710                 715                 720 


Gln Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly 
                725                 730                 735     


Ile Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn 
            740                 745                 750         


Asp Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Ser 
        755                 760                 765             


Leu Ile Asn Lys Asn Ile Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr 
    770                 775                 780                 


Ser Gly Asp Gly Ser Phe Val Val Glu Leu Lys Lys Arg Arg Ser Pro 
785                 790                 795                 800 


Val Lys Val Gly Val Arg Leu Arg Phe Ser Ile Thr Gln His Ile Arg 
                805                 810                 815     


Asp Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Arg 
            820                 825                 830         


Ile Val Glu Asn Asn Lys Ser Glu His Ser Trp Leu Glu Phe Ile Val 
        835                 840                 845             


Thr Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu 
    850                 855                 860                 


Asn Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys 
865                 870                 875                 880 


Val Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu 
                885                 890                 895     


Asp Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg 
            900                 905                 910 


<210>  59
<211>  3474
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - high activity Cb1-b megaTAL-Trex2 DNA construct

<400>  59
atgggatcct gcaggccacc taagaagaaa cgcaaagtcg tggatctacg cacgctcggc       60

tacagtcagc agcagcaaga gaagatcaaa ccgaaggtgc gttcgacagt ggcgcagcac      120

cacgaggcac tggtgggcca tgggtttaca cacgcgcaca tcgttgcgct cagccaacac      180

ccggcagcgt tagggaccgt cgctgtcacg tatcagcaca taatcacggc gttgccagag      240

gcgacacacg aagacatcgt tggcgtcggc aaacagtggt ccggcgcacg cgccctggag      300

gccttgctca cggatgcggg ggagttgaga ggtccgccgt tacagttgga cacaggccaa      360

cttgtgaaga ttgcaaaacg tggcggcgtg accgcaatgg aggcagtgca tgcatcgcgc      420

aatgcactga cgggtgcccc cctgaactta acacccgatc aagtggtagc gatagcgtca      480

aacggcgggg gtaaacaggc tttggagacg gtacagcggt tattgccggt actctgccag      540

gaccacggat tgacaccgga ccaagtggtg gcgattgcgt ccaacaacgg aggcaagcag      600

gcactagaga ctgtccaacg gcttcttccc gttctttgtc aggatcatgg gctaacccct      660

gatcaggtag tcgctatagc ttcaaatgga gggggcaagc aagcactgga gactgttcaa      720

cgactcctgc cagtgctctg ccaagaccac ggacttacac cagatcaagt ggttgctatt      780

gcctccaatg gtggcgggaa acaagcgttg gaaactgtgc agagactgtt acctgtcttg      840

tgtcaagacc acggcctcac gccagatcag gtggtagcca tagcgtcgaa cattggtggt      900

aagcaagccc ttgaaacggt ccagcgtctt ctgccggtgt tgtgccagga ccacggacta      960

acgccggatc aggtcgtagc cattgcttca aatgggggcg gcaaacaggc gctagagaca     1020

gtccagcgcc tcttgcctgt gttatgccag gatcacggct taaccccaga ccaagttgtg     1080

gctattgcat ctaacaatgg tggcaaacaa gccttggaga cagtgcaacg attactgcct     1140

gtcttatgtc aggatcatgg cctgacgccc gatcaggtag tggcaatcgc atctaacatt     1200

ggaggtaagc aagcactgga gactgtccag agattgttac ccgtactatg tcaagatcat     1260

ggtttgacgc ctgatcaggt tgttgcgata gccagcaata acggagggaa acaggctctt     1320

gaaaccgtac agcgacttct cccagtcttg tgccaagatc acgggcttac tcctgatcaa     1380

gtcgtagcta tcgccagcaa taacggtgga aaacaggccc tggaaaccgt acaacgtctc     1440

ctcccagtac tttgtcaaga ccacgggttg actccggatc aagtcgtcgc gatcgcgagc     1500

aatggtgggg ggaagcaggc gctggaaact gttcagagac tgctgcctgt actttgtcag     1560

gaccatggtc tgacacctga ccaagttgtg gcgatagcca gtaatatcgg gggaaaacag     1620

gcactagaga gcattgtggc ccagctgagc cggcctgatc cggcgttggc cgcgttgacc     1680

aacgaccacc tcgtcgcctt ggcctgcctc ggcggacgtc ctgccatgga tgcagtgaaa     1740

aagggattgc cgcacgcgcc ggaattgatc agaagagtca atcgccgtat tggcgaacgc     1800

acgtcccatc gcgttgcgat atctagagtg ggaggaagct cgcgcagaga gtccatcaac     1860

ccatggattc tgactggttt cgctgatgcc gaaggatgct tccgactaga catccgcaac     1920

gcaaacgatt taagagccgg atacagaact agactggcct tcgaaatcgt actgcacaac     1980

aaggacaaat cgattctgga gaatatccag tcgacttgga aggtcggcac aatctacaac     2040

gcgggcgaca acgcagtcag actgcaagtc acacgtttcg aagatttgaa agtgattatc     2100

gaccacttcg agaaatatcc gctgataaca cagaaattgg gcgattacaa gttgtttaaa     2160

caggcattca gcgtcatgga gaacaaagaa catcttaagg agaatgggat taaggagctc     2220

gtacgaatca aagctaagat gaattggggt ctcaatgacg aattgaaaaa agcatttcca     2280

gagaacatta gcaaagagcg ctcccttatc aataagaaca ttccgaatct caaatggctg     2340

gctggattca catctggtga cggctcgttc gtggtggaac taaagaagag aagaagcccc     2400

gtcaaggtag gagtgcggct gcgattcagc atcacccagc acatcagaga caagaacctg     2460

atgaattcat tgataacata cctaggctgt ggtcgtatcg ttgagaataa caaatctgag     2520

cacagttggc tcgaattcat tgtaacaaaa ttcagcgata tcaacgacaa gatcattccg     2580

gtattccagg aaaatactct gattggcgtc aaactcgagg actttgaaga ttggtgcaag     2640

gttgccaaat tgatcgaaga gaagaaacac ctgaccgaat ccggtttgga tgagattaag     2700

aaaatcaagc tgaacatgaa caaaggtcgt ggtggaggcg gtagcggagg cggagggtcg     2760

gctagctccg aagcaccgag agccgaaacc ttcgtgttcc tcgacctgga agccactggc     2820

ctgcccagtg tggagcccga gattgccgag ctgtccctgt tcgccgttca tcggtcctcc     2880

ctggaaaacc ctgagcacga cgagagcgga gcactggtgc tgccaagggt gctggacaag     2940

ctgaccctgt gcatgtgtcc cgagcgtccc ttcactgcca aggcatccga aatcactggt     3000

ctgagttcgg aagggctggc tcgttgtaga aaggctggat tcgatggtgc cgtggttcgg     3060

actttgcagg cattcctgtc caggcaggca ggtccaatct gcttggtcgc ccacaacggt     3120

tttgactacg acttcccact gctctgtgcc gaactgcgtc gcctgggagc acgccttcct     3180

cgggataccg tgtgcctgga caccctaccc gctctgcgtg gactggacag agcccactca     3240

cacggcacta gggcgagagg acgccagggt tacagcctcg gttcactgtt ccatcggtat     3300

ttcagggcag agccttccgc agcccattca gccgaaggcg atgtccacac cttgcttctg     3360

atcttcctgc atcgtgcagc agaactgctc gcctgggcag atgagcaggc tcgtggatgg     3420

gctcacattg agccgatgta cttgccacct gatgacccta gcctggaagc ctga           3474


<210>  60
<211>  3474
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - high activity Cb1-b megaTAL-Trex2 RNA construct

<400>  60
augggauccu gcaggccacc uaagaagaaa cgcaaagucg uggaucuacg cacgcucggc       60

uacagucagc agcagcaaga gaagaucaaa ccgaaggugc guucgacagu ggcgcagcac      120

cacgaggcac uggugggcca uggguuuaca cacgcgcaca ucguugcgcu cagccaacac      180

ccggcagcgu uagggaccgu cgcugucacg uaucagcaca uaaucacggc guugccagag      240

gcgacacacg aagacaucgu uggcgucggc aaacaguggu ccggcgcacg cgcccuggag      300

gccuugcuca cggaugcggg ggaguugaga gguccgccgu uacaguugga cacaggccaa      360

cuugugaaga uugcaaaacg uggcggcgug accgcaaugg aggcagugca ugcaucgcgc      420

aaugcacuga cgggugcccc ccugaacuua acacccgauc aagugguagc gauagcguca      480

aacggcgggg guaaacaggc uuuggagacg guacagcggu uauugccggu acucugccag      540

gaccacggau ugacaccgga ccaaguggug gcgauugcgu ccaacaacgg aggcaagcag      600

gcacuagaga cuguccaacg gcuucuuccc guucuuuguc aggaucaugg gcuaaccccu      660

gaucagguag ucgcuauagc uucaaaugga gggggcaagc aagcacugga gacuguucaa      720

cgacuccugc cagugcucug ccaagaccac ggacuuacac cagaucaagu gguugcuauu      780

gccuccaaug guggcgggaa acaagcguug gaaacugugc agagacuguu accugucuug      840

ugucaagacc acggccucac gccagaucag gugguagcca uagcgucgaa cauugguggu      900

aagcaagccc uugaaacggu ccagcgucuu cugccggugu ugugccagga ccacggacua      960

acgccggauc aggucguagc cauugcuuca aaugggggcg gcaaacaggc gcuagagaca     1020

guccagcgcc ucuugccugu guuaugccag gaucacggcu uaaccccaga ccaaguugug     1080

gcuauugcau cuaacaaugg uggcaaacaa gccuuggaga cagugcaacg auuacugccu     1140

gucuuauguc aggaucaugg ccugacgccc gaucagguag uggcaaucgc aucuaacauu     1200

ggagguaagc aagcacugga gacuguccag agauuguuac ccguacuaug ucaagaucau     1260

gguuugacgc cugaucaggu uguugcgaua gccagcaaua acggagggaa acaggcucuu     1320

gaaaccguac agcgacuucu cccagucuug ugccaagauc acgggcuuac uccugaucaa     1380

gucguagcua ucgccagcaa uaacggugga aaacaggccc uggaaaccgu acaacgucuc     1440

cucccaguac uuugucaaga ccacggguug acuccggauc aagucgucgc gaucgcgagc     1500

aauggugggg ggaagcaggc gcuggaaacu guucagagac ugcugccugu acuuugucag     1560

gaccaugguc ugacaccuga ccaaguugug gcgauagcca guaauaucgg gggaaaacag     1620

gcacuagaga gcauuguggc ccagcugagc cggccugauc cggcguuggc cgcguugacc     1680

aacgaccacc ucgucgccuu ggccugccuc ggcggacguc cugccaugga ugcagugaaa     1740

aagggauugc cgcacgcgcc ggaauugauc agaagaguca aucgccguau uggcgaacgc     1800

acgucccauc gcguugcgau aucuagagug ggaggaagcu cgcgcagaga guccaucaac     1860

ccauggauuc ugacugguuu cgcugaugcc gaaggaugcu uccgacuaga cauccgcaac     1920

gcaaacgauu uaagagccgg auacagaacu agacuggccu ucgaaaucgu acugcacaac     1980

aaggacaaau cgauucugga gaauauccag ucgacuugga aggucggcac aaucuacaac     2040

gcgggcgaca acgcagucag acugcaaguc acacguuucg aagauuugaa agugauuauc     2100

gaccacuucg agaaauaucc gcugauaaca cagaaauugg gcgauuacaa guuguuuaaa     2160

caggcauuca gcgucaugga gaacaaagaa caucuuaagg agaaugggau uaaggagcuc     2220

guacgaauca aagcuaagau gaauuggggu cucaaugacg aauugaaaaa agcauuucca     2280

gagaacauua gcaaagagcg cucccuuauc aauaagaaca uuccgaaucu caaauggcug     2340

gcuggauuca caucugguga cggcucguuc gugguggaac uaaagaagag aagaagcccc     2400

gucaagguag gagugcggcu gcgauucagc aucacccagc acaucagaga caagaaccug     2460

augaauucau ugauaacaua ccuaggcugu ggucguaucg uugagaauaa caaaucugag     2520

cacaguuggc ucgaauucau uguaacaaaa uucagcgaua ucaacgacaa gaucauuccg     2580

guauuccagg aaaauacucu gauuggcguc aaacucgagg acuuugaaga uuggugcaag     2640

guugccaaau ugaucgaaga gaagaaacac cugaccgaau ccgguuugga ugagauuaag     2700

aaaaucaagc ugaacaugaa caaaggucgu gguggaggcg guagcggagg cggagggucg     2760

gcuagcuccg aagcaccgag agccgaaacc uucguguucc ucgaccugga agccacuggc     2820

cugcccagug uggagcccga gauugccgag cugucccugu ucgccguuca ucgguccucc     2880

cuggaaaacc cugagcacga cgagagcgga gcacuggugc ugccaagggu gcuggacaag     2940

cugacccugu gcaugugucc cgagcguccc uucacugcca aggcauccga aaucacuggu     3000

cugaguucgg aagggcuggc ucguuguaga aaggcuggau ucgauggugc cgugguucgg     3060

acuuugcagg cauuccuguc caggcaggca gguccaaucu gcuuggucgc ccacaacggu     3120

uuugacuacg acuucccacu gcucugugcc gaacugcguc gccugggagc acgccuuccu     3180

cgggauaccg ugugccugga cacccuaccc gcucugcgug gacuggacag agcccacuca     3240

cacggcacua gggcgagagg acgccagggu uacagccucg guucacuguu ccaucgguau     3300

uucagggcag agccuuccgc agcccauuca gccgaaggcg auguccacac cuugcuucug     3360

aucuuccugc aucgugcagc agaacugcuc gccugggcag augagcaggc ucguggaugg     3420

gcucacauug agccgaugua cuugccaccu gaugacccua gccuggaagc cuga           3474


<210>  61
<211>  1157
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - high activity Cb1-b megaTAL-Trex2 protein construct

<400>  61

Met Gly Ser Cys Arg Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            500                 505                 510         


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
        515                 520                 525             


Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser 
    530                 535                 540                 


Ile Val Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr 
545                 550                 555                 560 


Asn Asp His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met 
                565                 570                 575     


Asp Ala Val Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg 
            580                 585                 590         


Val Asn Arg Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser 
        595                 600                 605             


Arg Val Gly Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu 
    610                 615                 620                 


Thr Gly Phe Ala Asp Ala Glu Gly Cys Phe Arg Leu Asp Ile Arg Asn 
625                 630                 635                 640 


Ala Asn Asp Leu Arg Ala Gly Tyr Arg Thr Arg Leu Ala Phe Glu Ile 
                645                 650                 655     


Val Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr 
            660                 665                 670         


Trp Lys Val Gly Thr Ile Tyr Asn Ala Gly Asp Asn Ala Val Arg Leu 
        675                 680                 685             


Gln Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu 
    690                 695                 700                 


Lys Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys 
705                 710                 715                 720 


Gln Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly 
                725                 730                 735     


Ile Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn 
            740                 745                 750         


Asp Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Ser 
        755                 760                 765             


Leu Ile Asn Lys Asn Ile Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr 
    770                 775                 780                 


Ser Gly Asp Gly Ser Phe Val Val Glu Leu Lys Lys Arg Arg Ser Pro 
785                 790                 795                 800 


Val Lys Val Gly Val Arg Leu Arg Phe Ser Ile Thr Gln His Ile Arg 
                805                 810                 815     


Asp Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Arg 
            820                 825                 830         


Ile Val Glu Asn Asn Lys Ser Glu His Ser Trp Leu Glu Phe Ile Val 
        835                 840                 845             


Thr Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu 
    850                 855                 860                 


Asn Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys 
865                 870                 875                 880 


Val Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu 
                885                 890                 895     


Asp Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Gly Gly 
            900                 905                 910         


Gly Gly Ser Gly Gly Gly Gly Ser Ala Ser Ser Glu Ala Pro Arg Ala 
        915                 920                 925             


Glu Thr Phe Val Phe Leu Asp Leu Glu Ala Thr Gly Leu Pro Ser Val 
    930                 935                 940                 


Glu Pro Glu Ile Ala Glu Leu Ser Leu Phe Ala Val His Arg Ser Ser 
945                 950                 955                 960 


Leu Glu Asn Pro Glu His Asp Glu Ser Gly Ala Leu Val Leu Pro Arg 
                965                 970                 975     


Val Leu Asp Lys Leu Thr Leu Cys Met Cys Pro Glu Arg Pro Phe Thr 
            980                 985                 990         


Ala Lys Ala Ser Glu Ile Thr Gly  Leu Ser Ser Glu Gly  Leu Ala Arg 
        995                 1000                 1005             


Cys Arg  Lys Ala Gly Phe Asp  Gly Ala Val Val Arg  Thr Leu Gln 
    1010                 1015                 1020             


Ala Phe  Leu Ser Arg Gln Ala  Gly Pro Ile Cys Leu  Val Ala His 
    1025                 1030                 1035             


Asn Gly  Phe Asp Tyr Asp Phe  Pro Leu Leu Cys Ala  Glu Leu Arg 
    1040                 1045                 1050             


Arg Leu  Gly Ala Arg Leu Pro  Arg Asp Thr Val Cys  Leu Asp Thr 
    1055                 1060                 1065             


Leu Pro  Ala Leu Arg Gly Leu  Asp Arg Ala His Ser  His Gly Thr 
    1070                 1075                 1080             


Arg Ala  Arg Gly Arg Gln Gly  Tyr Ser Leu Gly Ser  Leu Phe His 
    1085                 1090                 1095             


Arg Tyr  Phe Arg Ala Glu Pro  Ser Ala Ala His Ser  Ala Glu Gly 
    1100                 1105                 1110             


Asp Val  His Thr Leu Leu Leu  Ile Phe Leu His Arg  Ala Ala Glu 
    1115                 1120                 1125             


Leu Leu  Ala Trp Ala Asp Glu  Gln Ala Arg Gly Trp  Ala His Ile 
    1130                 1135                 1140             


Glu Pro  Met Tyr Leu Pro Pro  Asp Asp Pro Ser Leu  Glu Ala 
    1145                 1150                 1155         


<210>  62
<211>  3426
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - high activity Cb1-b megaTAL-ExoX DNA construct

<400>  62
atgggatcct gcaggccacc taagaagaaa cgcaaagtcg tggatctacg cacgctcggc       60

tacagtcagc agcagcaaga gaagatcaaa ccgaaggtgc gttcgacagt ggcgcagcac      120

cacgaggcac tggtgggcca tgggtttaca cacgcgcaca tcgttgcgct cagccaacac      180

ccggcagcgt tagggaccgt cgctgtcacg tatcagcaca taatcacggc gttgccagag      240

gcgacacacg aagacatcgt tggcgtcggc aaacagtggt ccggcgcacg cgccctggag      300

gccttgctca cggatgcggg ggagttgaga ggtccgccgt tacagttgga cacaggccaa      360

cttgtgaaga ttgcaaaacg tggcggcgtg accgcaatgg aggcagtgca tgcatcgcgc      420

aatgcactga cgggtgcccc cctgaactta acacccgatc aagtggtagc gatagcgtca      480

aacggcgggg gtaaacaggc tttggagacg gtacagcggt tattgccggt actctgccag      540

gaccacggat tgacaccgga ccaagtggtg gcgattgcgt ccaacaacgg aggcaagcag      600

gcactagaga ctgtccaacg gcttcttccc gttctttgtc aggatcatgg gctaacccct      660

gatcaggtag tcgctatagc ttcaaatgga gggggcaagc aagcactgga gactgttcaa      720

cgactcctgc cagtgctctg ccaagaccac ggacttacac cagatcaagt ggttgctatt      780

gcctccaatg gtggcgggaa acaagcgttg gaaactgtgc agagactgtt acctgtcttg      840

tgtcaagacc acggcctcac gccagatcag gtggtagcca tagcgtcgaa cattggtggt      900

aagcaagccc ttgaaacggt ccagcgtctt ctgccggtgt tgtgccagga ccacggacta      960

acgccggatc aggtcgtagc cattgcttca aatgggggcg gcaaacaggc gctagagaca     1020

gtccagcgcc tcttgcctgt gttatgccag gatcacggct taaccccaga ccaagttgtg     1080

gctattgcat ctaacaatgg tggcaaacaa gccttggaga cagtgcaacg attactgcct     1140

gtcttatgtc aggatcatgg cctgacgccc gatcaggtag tggcaatcgc atctaacatt     1200

ggaggtaagc aagcactgga gactgtccag agattgttac ccgtactatg tcaagatcat     1260

ggtttgacgc ctgatcaggt tgttgcgata gccagcaata acggagggaa acaggctctt     1320

gaaaccgtac agcgacttct cccagtcttg tgccaagatc acgggcttac tcctgatcaa     1380

gtcgtagcta tcgccagcaa taacggtgga aaacaggccc tggaaaccgt acaacgtctc     1440

ctcccagtac tttgtcaaga ccacgggttg actccggatc aagtcgtcgc gatcgcgagc     1500

aatggtgggg ggaagcaggc gctggaaact gttcagagac tgctgcctgt actttgtcag     1560

gaccatggtc tgacacctga ccaagttgtg gcgatagcca gtaatatcgg gggaaaacag     1620

gcactagaga gcattgtggc ccagctgagc cggcctgatc cggcgttggc cgcgttgacc     1680

aacgaccacc tcgtcgcctt ggcctgcctc ggcggacgtc ctgccatgga tgcagtgaaa     1740

aagggattgc cgcacgcgcc ggaattgatc agaagagtca atcgccgtat tggcgaacgc     1800

acgtcccatc gcgttgcgat atctagagtg ggaggaagct cgcgcagaga gtccatcaac     1860

ccatggattc tgactggttt cgctgatgcc gaaggatgct tccgactaga catccgcaac     1920

gcaaacgatt taagagccgg atacagaact agactggcct tcgaaatcgt actgcacaac     1980

aaggacaaat cgattctgga gaatatccag tcgacttgga aggtcggcac aatctacaac     2040

gcgggcgaca acgcagtcag actgcaagtc acacgtttcg aagatttgaa agtgattatc     2100

gaccacttcg agaaatatcc gctgataaca cagaaattgg gcgattacaa gttgtttaaa     2160

caggcattca gcgtcatgga gaacaaagaa catcttaagg agaatgggat taaggagctc     2220

gtacgaatca aagctaagat gaattggggt ctcaatgacg aattgaaaaa agcatttcca     2280

gagaacatta gcaaagagcg ctcccttatc aataagaaca ttccgaatct caaatggctg     2340

gctggattca catctggtga cggctcgttc gtggtggaac taaagaagag aagaagcccc     2400

gtcaaggtag gagtgcggct gcgattcagc atcacccagc acatcagaga caagaacctg     2460

atgaattcat tgataacata cctaggctgt ggtcgtatcg ttgagaataa caaatctgag     2520

cacagttggc tcgaattcat tgtaacaaaa ttcagcgata tcaacgacaa gatcattccg     2580

gtattccagg aaaatactct gattggcgtc aaactcgagg actttgaaga ttggtgcaag     2640

gttgccaaat tgatcgaaga gaagaaacac ctgaccgaat ccggtttgga tgagattaag     2700

aaaatcaagc tgaacatgaa caaaggtcgt ggtggaggcg gtagcggagg cggagggtcg     2760

gctagcctgc ggatcataga cacggaaacc tgtggactcc agggtggtat cgtggagata     2820

gccagtgtcg atgtaattga tgggaaaatc gtaaacccga tgagccacct cgtccgaccg     2880

gataggccga taagtccgca ggctatggct atacatagaa tcacggaggc tatggtagct     2940

gacaagcctt ggatcgagga cgttatccca cactactatg ggagcgaatg gtatgtagcg     3000

cacaacgcat catttgatag gcgcgtgctt cccgaaatgc ctggggagtg gatttgcaca     3060

atgaaactcg ctcgccgact gtggcctggt atcaagtata gtaacatggc actttacaag     3120

acccgcaaac tcaacgtaca gacacctccg ggtctccacc accatagggc actctatgat     3180

tgttacataa ccgctgctct ccttatagac ataatgaata cctccgggtg gaccgccgaa     3240

caaatggcag acataacggg aagacctagt ttgatgacaa cctttacatt tggaaagtat     3300

cggggcaagg cggtgagcga tgtggccgaa agagatcccg gttatcttag atggctcttc     3360

aataacttgg attctatgtc acctgaactt cgccttaccc tgaagcatta ccttgagaat     3420

acctga                                                                3426


<210>  63
<211>  3426
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - high activity Cb1-b megaTAL-ExoX  RNA construct

<400>  63
augggauccu gcaggccacc uaagaagaaa cgcaaagucg uggaucuacg cacgcucggc       60

uacagucagc agcagcaaga gaagaucaaa ccgaaggugc guucgacagu ggcgcagcac      120

cacgaggcac uggugggcca uggguuuaca cacgcgcaca ucguugcgcu cagccaacac      180

ccggcagcgu uagggaccgu cgcugucacg uaucagcaca uaaucacggc guugccagag      240

gcgacacacg aagacaucgu uggcgucggc aaacaguggu ccggcgcacg cgcccuggag      300

gccuugcuca cggaugcggg ggaguugaga gguccgccgu uacaguugga cacaggccaa      360

cuugugaaga uugcaaaacg uggcggcgug accgcaaugg aggcagugca ugcaucgcgc      420

aaugcacuga cgggugcccc ccugaacuua acacccgauc aagugguagc gauagcguca      480

aacggcgggg guaaacaggc uuuggagacg guacagcggu uauugccggu acucugccag      540

gaccacggau ugacaccgga ccaaguggug gcgauugcgu ccaacaacgg aggcaagcag      600

gcacuagaga cuguccaacg gcuucuuccc guucuuuguc aggaucaugg gcuaaccccu      660

gaucagguag ucgcuauagc uucaaaugga gggggcaagc aagcacugga gacuguucaa      720

cgacuccugc cagugcucug ccaagaccac ggacuuacac cagaucaagu gguugcuauu      780

gccuccaaug guggcgggaa acaagcguug gaaacugugc agagacuguu accugucuug      840

ugucaagacc acggccucac gccagaucag gugguagcca uagcgucgaa cauugguggu      900

aagcaagccc uugaaacggu ccagcgucuu cugccggugu ugugccagga ccacggacua      960

acgccggauc aggucguagc cauugcuuca aaugggggcg gcaaacaggc gcuagagaca     1020

guccagcgcc ucuugccugu guuaugccag gaucacggcu uaaccccaga ccaaguugug     1080

gcuauugcau cuaacaaugg uggcaaacaa gccuuggaga cagugcaacg auuacugccu     1140

gucuuauguc aggaucaugg ccugacgccc gaucagguag uggcaaucgc aucuaacauu     1200

ggagguaagc aagcacugga gacuguccag agauuguuac ccguacuaug ucaagaucau     1260

gguuugacgc cugaucaggu uguugcgaua gccagcaaua acggagggaa acaggcucuu     1320

gaaaccguac agcgacuucu cccagucuug ugccaagauc acgggcuuac uccugaucaa     1380

gucguagcua ucgccagcaa uaacggugga aaacaggccc uggaaaccgu acaacgucuc     1440

cucccaguac uuugucaaga ccacggguug acuccggauc aagucgucgc gaucgcgagc     1500

aauggugggg ggaagcaggc gcuggaaacu guucagagac ugcugccugu acuuugucag     1560

gaccaugguc ugacaccuga ccaaguugug gcgauagcca guaauaucgg gggaaaacag     1620

gcacuagaga gcauuguggc ccagcugagc cggccugauc cggcguuggc cgcguugacc     1680

aacgaccacc ucgucgccuu ggccugccuc ggcggacguc cugccaugga ugcagugaaa     1740

aagggauugc cgcacgcgcc ggaauugauc agaagaguca aucgccguau uggcgaacgc     1800

acgucccauc gcguugcgau aucuagagug ggaggaagcu cgcgcagaga guccaucaac     1860

ccauggauuc ugacugguuu cgcugaugcc gaaggaugcu uccgacuaga cauccgcaac     1920

gcaaacgauu uaagagccgg auacagaacu agacuggccu ucgaaaucgu acugcacaac     1980

aaggacaaau cgauucugga gaauauccag ucgacuugga aggucggcac aaucuacaac     2040

gcgggcgaca acgcagucag acugcaaguc acacguuucg aagauuugaa agugauuauc     2100

gaccacuucg agaaauaucc gcugauaaca cagaaauugg gcgauuacaa guuguuuaaa     2160

caggcauuca gcgucaugga gaacaaagaa caucuuaagg agaaugggau uaaggagcuc     2220

guacgaauca aagcuaagau gaauuggggu cucaaugacg aauugaaaaa agcauuucca     2280

gagaacauua gcaaagagcg cucccuuauc aauaagaaca uuccgaaucu caaauggcug     2340

gcuggauuca caucugguga cggcucguuc gugguggaac uaaagaagag aagaagcccc     2400

gucaagguag gagugcggcu gcgauucagc aucacccagc acaucagaga caagaaccug     2460

augaauucau ugauaacaua ccuaggcugu ggucguaucg uugagaauaa caaaucugag     2520

cacaguuggc ucgaauucau uguaacaaaa uucagcgaua ucaacgacaa gaucauuccg     2580

guauuccagg aaaauacucu gauuggcguc aaacucgagg acuuugaaga uuggugcaag     2640

guugccaaau ugaucgaaga gaagaaacac cugaccgaau ccgguuugga ugagauuaag     2700

aaaaucaagc ugaacaugaa caaaggucgu gguggaggcg guagcggagg cggagggucg     2760

gcuagccugc ggaucauaga cacggaaacc uguggacucc agggugguau cguggagaua     2820

gccagugucg auguaauuga ugggaaaauc guaaacccga ugagccaccu cguccgaccg     2880

gauaggccga uaaguccgca ggcuauggcu auacauagaa ucacggaggc uaugguagcu     2940

gacaagccuu ggaucgagga cguuauccca cacuacuaug ggagcgaaug guauguagcg     3000

cacaacgcau cauuugauag gcgcgugcuu cccgaaaugc cuggggagug gauuugcaca     3060

augaaacucg cucgccgacu guggccuggu aucaaguaua guaacauggc acuuuacaag     3120

acccgcaaac ucaacguaca gacaccuccg ggucuccacc accauagggc acucuaugau     3180

uguuacauaa ccgcugcucu ccuuauagac auaaugaaua ccuccgggug gaccgccgaa     3240

caaauggcag acauaacggg aagaccuagu uugaugacaa ccuuuacauu uggaaaguau     3300

cggggcaagg cggugagcga uguggccgaa agagaucccg guuaucuuag auggcucuuc     3360

aauaacuugg auucuauguc accugaacuu cgccuuaccc ugaagcauua ccuugagaau     3420

accuga                                                                3426


<210>  64
<211>  1141
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - high activity Cb1-b megaTAL-ExoX protein construct

<400>  64

Met Gly Ser Cys Arg Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            500                 505                 510         


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
        515                 520                 525             


Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser 
    530                 535                 540                 


Ile Val Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr 
545                 550                 555                 560 


Asn Asp His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met 
                565                 570                 575     


Asp Ala Val Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg 
            580                 585                 590         


Val Asn Arg Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser 
        595                 600                 605             


Arg Val Gly Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu 
    610                 615                 620                 


Thr Gly Phe Ala Asp Ala Glu Gly Cys Phe Arg Leu Asp Ile Arg Asn 
625                 630                 635                 640 


Ala Asn Asp Leu Arg Ala Gly Tyr Arg Thr Arg Leu Ala Phe Glu Ile 
                645                 650                 655     


Val Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr 
            660                 665                 670         


Trp Lys Val Gly Thr Ile Tyr Asn Ala Gly Asp Asn Ala Val Arg Leu 
        675                 680                 685             


Gln Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu 
    690                 695                 700                 


Lys Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys 
705                 710                 715                 720 


Gln Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly 
                725                 730                 735     


Ile Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn 
            740                 745                 750         


Asp Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Ser 
        755                 760                 765             


Leu Ile Asn Lys Asn Ile Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr 
    770                 775                 780                 


Ser Gly Asp Gly Ser Phe Val Val Glu Leu Lys Lys Arg Arg Ser Pro 
785                 790                 795                 800 


Val Lys Val Gly Val Arg Leu Arg Phe Ser Ile Thr Gln His Ile Arg 
                805                 810                 815     


Asp Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Arg 
            820                 825                 830         


Ile Val Glu Asn Asn Lys Ser Glu His Ser Trp Leu Glu Phe Ile Val 
        835                 840                 845             


Thr Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu 
    850                 855                 860                 


Asn Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys 
865                 870                 875                 880 


Val Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu 
                885                 890                 895     


Asp Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Gly Gly 
            900                 905                 910         


Gly Gly Ser Gly Gly Gly Gly Ser Ala Ser Leu Arg Ile Ile Asp Thr 
        915                 920                 925             


Glu Thr Cys Gly Leu Gln Gly Gly Ile Val Glu Ile Ala Ser Val Asp 
    930                 935                 940                 


Val Ile Asp Gly Lys Ile Val Asn Pro Met Ser His Leu Val Arg Pro 
945                 950                 955                 960 


Asp Arg Pro Ile Ser Pro Gln Ala Met Ala Ile His Arg Ile Thr Glu 
                965                 970                 975     


Ala Met Val Ala Asp Lys Pro Trp Ile Glu Asp Val Ile Pro His Tyr 
            980                 985                 990         


Tyr Gly Ser Glu Trp Tyr Val Ala  His Asn Ala Ser Phe  Asp Arg Arg 
        995                 1000                 1005             


Val Leu  Pro Glu Met Pro Gly  Glu Trp Ile Cys Thr  Met Lys Leu 
    1010                 1015                 1020             


Ala Arg  Arg Leu Trp Pro Gly  Ile Lys Tyr Ser Asn  Met Ala Leu 
    1025                 1030                 1035             


Tyr Lys  Thr Arg Lys Leu Asn  Val Gln Thr Pro Pro  Gly Leu His 
    1040                 1045                 1050             


His His  Arg Ala Leu Tyr Asp  Cys Tyr Ile Thr Ala  Ala Leu Leu 
    1055                 1060                 1065             


Ile Asp  Ile Met Asn Thr Ser  Gly Trp Thr Ala Glu  Gln Met Ala 
    1070                 1075                 1080             


Asp Ile  Thr Gly Arg Pro Ser  Leu Met Thr Thr Phe  Thr Phe Gly 
    1085                 1090                 1095             


Lys Tyr  Arg Gly Lys Ala Val  Ser Asp Val Ala Glu  Arg Asp Pro 
    1100                 1105                 1110             


Gly Tyr  Leu Arg Trp Leu Phe  Asn Asn Leu Asp Ser  Met Ser Pro 
    1115                 1120                 1125             


Glu Leu  Arg Leu Thr Leu Lys  His Tyr Leu Glu Asn  Thr 
    1130                 1135                 1140     


<210>  65
<211>  2748
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity Cb1-b megaTAL DNA construct

<400>  65
atgggatcct gcaggccacc taagaagaaa cgcaaagtcg tggatctacg cacgctcggc       60

tacagtcagc agcagcaaga gaagatcaaa ccgaaggtgc gttcgacagt ggcgcagcac      120

cacgaggcac tggtgggcca tgggtttaca cacgcgcaca tcgttgcgct cagccaacac      180

ccggcagcgt tagggaccgt cgctgtcacg tatcagcaca taatcacggc gttgccagag      240

gcgacacacg aagacatcgt tggcgtcggc aaacagtggt ccggcgcacg cgccctggag      300

gccttgctca cggatgcggg ggagttgaga ggtccgccgt tacagttgga cacaggccaa      360

cttgtgaaga ttgcaaaacg tggcggcgtg accgcaatgg aggcagtgca tgcatcgcgc      420

aatgcactga cgggtgcccc cctgaactta acacccgatc aagtggtagc gatagcgtca      480

aacggcgggg gtaaacaggc tttggagacg gtacagcggt tattgccggt actctgccag      540

gaccacggat tgacaccgga ccaagtggtg gcgattgcgt ccaacaacgg aggcaagcag      600

gcactagaga ctgtccaacg gcttcttccc gttctttgtc aggatcatgg gctaacccct      660

gatcaggtag tcgctatagc ttcaaatgga gggggcaagc aagcactgga gactgttcaa      720

cgactcctgc cagtgctctg ccaagaccac ggacttacac cagatcaagt ggttgctatt      780

gcctccaatg gtggcgggaa acaagcgttg gaaactgtgc agagactgtt acctgtcttg      840

tgtcaagacc acggcctcac gccagatcag gtggtagcca tagcgtcgaa cattggtggt      900

aagcaagccc ttgaaacggt ccagcgtctt ctgccggtgt tgtgccagga ccacggacta      960

acgccggatc aggtcgtagc cattgcttca aatgggggcg gcaaacaggc gctagagaca     1020

gtccagcgcc tcttgcctgt gttatgccag gatcacggct taaccccaga ccaagttgtg     1080

gctattgcat ctaacaatgg tggcaaacaa gccttggaga cagtgcaacg attactgcct     1140

gtcttatgtc aggatcatgg cctgacgccc gatcaggtag tggcaatcgc atctaacatt     1200

ggaggtaagc aagcactgga gactgtccag agattgttac ccgtactatg tcaagatcat     1260

ggtttgacgc ctgatcaggt tgttgcgata gccagcaata acggagggaa acaggctctt     1320

gaaaccgtac agcgacttct cccagtcttg tgccaagatc acgggcttac tcctgatcaa     1380

gtcgtagcta tcgccagcaa taacggtgga aaacaggccc tggaaaccgt acaacgtctc     1440

ctcccagtac tttgtcaaga ccacgggttg actccggatc aagtcgtcgc gatcgcgagc     1500

aatggtgggg ggaagcaggc gctggaaact gttcagagac tgctgcctgt actttgtcag     1560

gaccatggtc tgacacctga ccaagttgtg gcgatagcca gtaatatcgg gggaaaacag     1620

gcactagaga gcattgtggc ccagctgagc cggcctgatc cggcgttggc cgcgttgacc     1680

aacgaccacc tcgtcgcctt ggcctgcctc ggcggacgtc ctgccatgga tgcagtgaaa     1740

aagggattgc cgcacgcgcc ggaattgatc agaagagtca atcgccgtat tggcgaacgc     1800

acgtcccatc gcgttgcgat atctagagtg ggaggaagct cgcgcagaga gtccatcaac     1860

ccatggattc tgactggttt cgctgatgcc gaaggatgct tccgactaga catccgcaac     1920

gcaaactcca tgcgtgtaaa atacagaact agactgtcct tcgaaatcgt actgcacaac     1980

aaggacaaat cgattctgga gaatatccag tcgacttgga aggtcggcaa aatcacaaac     2040

tacggcgaca gaggagtccg cctgagagtc ggccgtttcg aagatttgaa agtgattatc     2100

gaccacttcg agaaatatcc gctgataaca cagaaattgg gcgattacaa gttgtttaaa     2160

caggcattca gcgtcatgga gaacaaagaa catcttaagg agaatgggat taaggagctc     2220

gtacgaatca aagctaagat gaattggggt ctcaatgacg aattgaaaaa agcatttcca     2280

gagaacatta gcaaagagcg cccccttatc aataagaaca tccccaattt caaatggctg     2340

gctggattca catctggtga cggctcgttc atggtggaac taatgaagaa taagaataac     2400

gttattgtac gtgtgcgtct gagattctca atctcccagc acatcagaga caagaacctg     2460

atgaattcat tgataacata cctaggctgt ggtcgtatcg ttgagaataa caaatctgag     2520

cacagttggc tcgaattcat tgtaacaaaa ttcagcgata tcaacgacaa gatcattccg     2580

gtattccagg aaaatactct gattggcgtc aaactcgagg actttgaaga ttggtgcaag     2640

gttgccaaat tgatcgaaga gaagaaacac ctgaccgaat ccggtttgga tgagattaag     2700

aaaatcaagc tgaacatgaa caaaggtcgt gtcttcagcg gccgctga                  2748


<210>  66
<211>  2748
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity Cb1-b megaTAL RNA construct

<400>  66
augggauccu gcaggccacc uaagaagaaa cgcaaagucg uggaucuacg cacgcucggc       60

uacagucagc agcagcaaga gaagaucaaa ccgaaggugc guucgacagu ggcgcagcac      120

cacgaggcac uggugggcca uggguuuaca cacgcgcaca ucguugcgcu cagccaacac      180

ccggcagcgu uagggaccgu cgcugucacg uaucagcaca uaaucacggc guugccagag      240

gcgacacacg aagacaucgu uggcgucggc aaacaguggu ccggcgcacg cgcccuggag      300

gccuugcuca cggaugcggg ggaguugaga gguccgccgu uacaguugga cacaggccaa      360

cuugugaaga uugcaaaacg uggcggcgug accgcaaugg aggcagugca ugcaucgcgc      420

aaugcacuga cgggugcccc ccugaacuua acacccgauc aagugguagc gauagcguca      480

aacggcgggg guaaacaggc uuuggagacg guacagcggu uauugccggu acucugccag      540

gaccacggau ugacaccgga ccaaguggug gcgauugcgu ccaacaacgg aggcaagcag      600

gcacuagaga cuguccaacg gcuucuuccc guucuuuguc aggaucaugg gcuaaccccu      660

gaucagguag ucgcuauagc uucaaaugga gggggcaagc aagcacugga gacuguucaa      720

cgacuccugc cagugcucug ccaagaccac ggacuuacac cagaucaagu gguugcuauu      780

gccuccaaug guggcgggaa acaagcguug gaaacugugc agagacuguu accugucuug      840

ugucaagacc acggccucac gccagaucag gugguagcca uagcgucgaa cauugguggu      900

aagcaagccc uugaaacggu ccagcgucuu cugccggugu ugugccagga ccacggacua      960

acgccggauc aggucguagc cauugcuuca aaugggggcg gcaaacaggc gcuagagaca     1020

guccagcgcc ucuugccugu guuaugccag gaucacggcu uaaccccaga ccaaguugug     1080

gcuauugcau cuaacaaugg uggcaaacaa gccuuggaga cagugcaacg auuacugccu     1140

gucuuauguc aggaucaugg ccugacgccc gaucagguag uggcaaucgc aucuaacauu     1200

ggagguaagc aagcacugga gacuguccag agauuguuac ccguacuaug ucaagaucau     1260

gguuugacgc cugaucaggu uguugcgaua gccagcaaua acggagggaa acaggcucuu     1320

gaaaccguac agcgacuucu cccagucuug ugccaagauc acgggcuuac uccugaucaa     1380

gucguagcua ucgccagcaa uaacggugga aaacaggccc uggaaaccgu acaacgucuc     1440

cucccaguac uuugucaaga ccacggguug acuccggauc aagucgucgc gaucgcgagc     1500

aauggugggg ggaagcaggc gcuggaaacu guucagagac ugcugccugu acuuugucag     1560

gaccaugguc ugacaccuga ccaaguugug gcgauagcca guaauaucgg gggaaaacag     1620

gcacuagaga gcauuguggc ccagcugagc cggccugauc cggcguuggc cgcguugacc     1680

aacgaccacc ucgucgccuu ggccugccuc ggcggacguc cugccaugga ugcagugaaa     1740

aagggauugc cgcacgcgcc ggaauugauc agaagaguca aucgccguau uggcgaacgc     1800

acgucccauc gcguugcgau aucuagagug ggaggaagcu cgcgcagaga guccaucaac     1860

ccauggauuc ugacugguuu cgcugaugcc gaaggaugcu uccgacuaga cauccgcaac     1920

gcaaacucca ugcguguaaa auacagaacu agacuguccu ucgaaaucgu acugcacaac     1980

aaggacaaau cgauucugga gaauauccag ucgacuugga aggucggcaa aaucacaaac     2040

uacggcgaca gaggaguccg ccugagaguc ggccguuucg aagauuugaa agugauuauc     2100

gaccacuucg agaaauaucc gcugauaaca cagaaauugg gcgauuacaa guuguuuaaa     2160

caggcauuca gcgucaugga gaacaaagaa caucuuaagg agaaugggau uaaggagcuc     2220

guacgaauca aagcuaagau gaauuggggu cucaaugacg aauugaaaaa agcauuucca     2280

gagaacauua gcaaagagcg cccccuuauc aauaagaaca uccccaauuu caaauggcug     2340

gcuggauuca caucugguga cggcucguuc augguggaac uaaugaagaa uaagaauaac     2400

guuauuguac gugugcgucu gagauucuca aucucccagc acaucagaga caagaaccug     2460

augaauucau ugauaacaua ccuaggcugu ggucguaucg uugagaauaa caaaucugag     2520

cacaguuggc ucgaauucau uguaacaaaa uucagcgaua ucaacgacaa gaucauuccg     2580

guauuccagg aaaauacucu gauuggcguc aaacucgagg acuuugaaga uuggugcaag     2640

guugccaaau ugaucgaaga gaagaaacac cugaccgaau ccgguuugga ugagauuaag     2700

aaaaucaagc ugaacaugaa caaaggucgu gucuucagcg gccgcuga                  2748


<210>  67
<211>  915
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity Cb1-b megaTAL protein construct

<400>  67

Met Gly Ser Cys Arg Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            500                 505                 510         


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
        515                 520                 525             


Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser 
    530                 535                 540                 


Ile Val Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr 
545                 550                 555                 560 


Asn Asp His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met 
                565                 570                 575     


Asp Ala Val Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg 
            580                 585                 590         


Val Asn Arg Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser 
        595                 600                 605             


Arg Val Gly Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu 
    610                 615                 620                 


Thr Gly Phe Ala Asp Ala Glu Gly Cys Phe Arg Leu Asp Ile Arg Asn 
625                 630                 635                 640 


Ala Asn Ser Met Arg Val Lys Tyr Arg Thr Arg Leu Ser Phe Glu Ile 
                645                 650                 655     


Val Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr 
            660                 665                 670         


Trp Lys Val Gly Lys Ile Thr Asn Tyr Gly Asp Arg Gly Val Arg Leu 
        675                 680                 685             


Arg Val Gly Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu 
    690                 695                 700                 


Lys Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys 
705                 710                 715                 720 


Gln Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly 
                725                 730                 735     


Ile Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn 
            740                 745                 750         


Asp Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro 
        755                 760                 765             


Leu Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr 
    770                 775                 780                 


Ser Gly Asp Gly Ser Phe Met Val Glu Leu Met Lys Asn Lys Asn Asn 
785                 790                 795                 800 


Val Ile Val Arg Val Arg Leu Arg Phe Ser Ile Ser Gln His Ile Arg 
                805                 810                 815     


Asp Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Arg 
            820                 825                 830         


Ile Val Glu Asn Asn Lys Ser Glu His Ser Trp Leu Glu Phe Ile Val 
        835                 840                 845             


Thr Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu 
    850                 855                 860                 


Asn Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys 
865                 870                 875                 880 


Val Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu 
                885                 890                 895     


Asp Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe 
            900                 905                 910         


Ser Gly Arg 
        915 


<210>  68
<211>  3474
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity Cb1-b megaTAL-Trex2 DNA construct

<400>  68
atgggatcct gcaggccacc taagaagaaa cgcaaagtcg tggatctacg cacgctcggc       60

tacagtcagc agcagcaaga gaagatcaaa ccgaaggtgc gttcgacagt ggcgcagcac      120

cacgaggcac tggtgggcca tgggtttaca cacgcgcaca tcgttgcgct cagccaacac      180

ccggcagcgt tagggaccgt cgctgtcacg tatcagcaca taatcacggc gttgccagag      240

gcgacacacg aagacatcgt tggcgtcggc aaacagtggt ccggcgcacg cgccctggag      300

gccttgctca cggatgcggg ggagttgaga ggtccgccgt tacagttgga cacaggccaa      360

cttgtgaaga ttgcaaaacg tggcggcgtg accgcaatgg aggcagtgca tgcatcgcgc      420

aatgcactga cgggtgcccc cctgaactta acacccgatc aagtggtagc gatagcgtca      480

aacggcgggg gtaaacaggc tttggagacg gtacagcggt tattgccggt actctgccag      540

gaccacggat tgacaccgga ccaagtggtg gcgattgcgt ccaacaacgg aggcaagcag      600

gcactagaga ctgtccaacg gcttcttccc gttctttgtc aggatcatgg gctaacccct      660

gatcaggtag tcgctatagc ttcaaatgga gggggcaagc aagcactgga gactgttcaa      720

cgactcctgc cagtgctctg ccaagaccac ggacttacac cagatcaagt ggttgctatt      780

gcctccaatg gtggcgggaa acaagcgttg gaaactgtgc agagactgtt acctgtcttg      840

tgtcaagacc acggcctcac gccagatcag gtggtagcca tagcgtcgaa cattggtggt      900

aagcaagccc ttgaaacggt ccagcgtctt ctgccggtgt tgtgccagga ccacggacta      960

acgccggatc aggtcgtagc cattgcttca aatgggggcg gcaaacaggc gctagagaca     1020

gtccagcgcc tcttgcctgt gttatgccag gatcacggct taaccccaga ccaagttgtg     1080

gctattgcat ctaacaatgg tggcaaacaa gccttggaga cagtgcaacg attactgcct     1140

gtcttatgtc aggatcatgg cctgacgccc gatcaggtag tggcaatcgc atctaacatt     1200

ggaggtaagc aagcactgga gactgtccag agattgttac ccgtactatg tcaagatcat     1260

ggtttgacgc ctgatcaggt tgttgcgata gccagcaata acggagggaa acaggctctt     1320

gaaaccgtac agcgacttct cccagtcttg tgccaagatc acgggcttac tcctgatcaa     1380

gtcgtagcta tcgccagcaa taacggtgga aaacaggccc tggaaaccgt acaacgtctc     1440

ctcccagtac tttgtcaaga ccacgggttg actccggatc aagtcgtcgc gatcgcgagc     1500

aatggtgggg ggaagcaggc gctggaaact gttcagagac tgctgcctgt actttgtcag     1560

gaccatggtc tgacacctga ccaagttgtg gcgatagcca gtaatatcgg gggaaaacag     1620

gcactagaga gcattgtggc ccagctgagc cggcctgatc cggcgttggc cgcgttgacc     1680

aacgaccacc tcgtcgcctt ggcctgcctc ggcggacgtc ctgccatgga tgcagtgaaa     1740

aagggattgc cgcacgcgcc ggaattgatc agaagagtca atcgccgtat tggcgaacgc     1800

acgtcccatc gcgttgcgat atctagagtg ggaggaagct cgcgcagaga gtccatcaac     1860

ccatggattc tgactggttt cgctgatgcc gaaggatgct tccgactaga catccgcaac     1920

gcaaactcca tgcgtgtaaa atacagaact agactgtcct tcgaaatcgt actgcacaac     1980

aaggacaaat cgattctgga gaatatccag tcgacttgga aggtcggcaa aatcacaaac     2040

tacggcgaca gaggagtccg cctgagagtc ggccgtttcg aagatttgaa agtgattatc     2100

gaccacttcg agaaatatcc gctgataaca cagaaattgg gcgattacaa gttgtttaaa     2160

caggcattca gcgtcatgga gaacaaagaa catcttaagg agaatgggat taaggagctc     2220

gtacgaatca aagctaagat gaattggggt ctcaatgacg aattgaaaaa agcatttcca     2280

gagaacatta gcaaagagcg cccccttatc aataagaaca tccccaattt caaatggctg     2340

gctggattca catctggtga cggctcgttc atggtggaac taatgaagaa taagaataac     2400

gttattgtac gtgtgcgtct gagattctca atctcccagc acatcagaga caagaacctg     2460

atgaattcat tgataacata cctaggctgt ggtcgtatcg ttgagaataa caaatctgag     2520

cacagttggc tcgaattcat tgtaacaaaa ttcagcgata tcaacgacaa gatcattccg     2580

gtattccagg aaaatactct gattggcgtc aaactcgagg actttgaaga ttggtgcaag     2640

gttgccaaat tgatcgaaga gaagaaacac ctgaccgaat ccggtttgga tgagattaag     2700

aaaatcaagc tgaacatgaa caaaggtcgt ggtggaggcg gtagcggagg cggagggtcg     2760

gctagctccg aagcaccgag agccgaaacc ttcgtgttcc tcgacctgga agccactggc     2820

ctgcccagtg tggagcccga gattgccgag ctgtccctgt tcgccgttca tcggtcctcc     2880

ctggaaaacc ctgagcacga cgagagcgga gcactggtgc tgccaagggt gctggacaag     2940

ctgaccctgt gcatgtgtcc cgagcgtccc ttcactgcca aggcatccga aatcactggt     3000

ctgagttcgg aagggctggc tcgttgtaga aaggctggat tcgatggtgc cgtggttcgg     3060

actttgcagg cattcctgtc caggcaggca ggtccaatct gcttggtcgc ccacaacggt     3120

tttgactacg acttcccact gctctgtgcc gaactgcgtc gcctgggagc acgccttcct     3180

cgggataccg tgtgcctgga caccctaccc gctctgcgtg gactggacag agcccactca     3240

cacggcacta gggcgagagg acgccagggt tacagcctcg gttcactgtt ccatcggtat     3300

ttcagggcag agccttccgc agcccattca gccgaaggcg atgtccacac cttgcttctg     3360

atcttcctgc atcgtgcagc agaactgctc gcctgggcag atgagcaggc tcgtggatgg     3420

gctcacattg agccgatgta cttgccacct gatgacccta gcctggaagc ctga           3474


<210>  69
<211>  3474
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity Cb1-b megaTAL-Trex2 RNA construct

<400>  69
augggauccu gcaggccacc uaagaagaaa cgcaaagucg uggaucuacg cacgcucggc       60

uacagucagc agcagcaaga gaagaucaaa ccgaaggugc guucgacagu ggcgcagcac      120

cacgaggcac uggugggcca uggguuuaca cacgcgcaca ucguugcgcu cagccaacac      180

ccggcagcgu uagggaccgu cgcugucacg uaucagcaca uaaucacggc guugccagag      240

gcgacacacg aagacaucgu uggcgucggc aaacaguggu ccggcgcacg cgcccuggag      300

gccuugcuca cggaugcggg ggaguugaga gguccgccgu uacaguugga cacaggccaa      360

cuugugaaga uugcaaaacg uggcggcgug accgcaaugg aggcagugca ugcaucgcgc      420

aaugcacuga cgggugcccc ccugaacuua acacccgauc aagugguagc gauagcguca      480

aacggcgggg guaaacaggc uuuggagacg guacagcggu uauugccggu acucugccag      540

gaccacggau ugacaccgga ccaaguggug gcgauugcgu ccaacaacgg aggcaagcag      600

gcacuagaga cuguccaacg gcuucuuccc guucuuuguc aggaucaugg gcuaaccccu      660

gaucagguag ucgcuauagc uucaaaugga gggggcaagc aagcacugga gacuguucaa      720

cgacuccugc cagugcucug ccaagaccac ggacuuacac cagaucaagu gguugcuauu      780

gccuccaaug guggcgggaa acaagcguug gaaacugugc agagacuguu accugucuug      840

ugucaagacc acggccucac gccagaucag gugguagcca uagcgucgaa cauugguggu      900

aagcaagccc uugaaacggu ccagcgucuu cugccggugu ugugccagga ccacggacua      960

acgccggauc aggucguagc cauugcuuca aaugggggcg gcaaacaggc gcuagagaca     1020

guccagcgcc ucuugccugu guuaugccag gaucacggcu uaaccccaga ccaaguugug     1080

gcuauugcau cuaacaaugg uggcaaacaa gccuuggaga cagugcaacg auuacugccu     1140

gucuuauguc aggaucaugg ccugacgccc gaucagguag uggcaaucgc aucuaacauu     1200

ggagguaagc aagcacugga gacuguccag agauuguuac ccguacuaug ucaagaucau     1260

gguuugacgc cugaucaggu uguugcgaua gccagcaaua acggagggaa acaggcucuu     1320

gaaaccguac agcgacuucu cccagucuug ugccaagauc acgggcuuac uccugaucaa     1380

gucguagcua ucgccagcaa uaacggugga aaacaggccc uggaaaccgu acaacgucuc     1440

cucccaguac uuugucaaga ccacggguug acuccggauc aagucgucgc gaucgcgagc     1500

aauggugggg ggaagcaggc gcuggaaacu guucagagac ugcugccugu acuuugucag     1560

gaccaugguc ugacaccuga ccaaguugug gcgauagcca guaauaucgg gggaaaacag     1620

gcacuagaga gcauuguggc ccagcugagc cggccugauc cggcguuggc cgcguugacc     1680

aacgaccacc ucgucgccuu ggccugccuc ggcggacguc cugccaugga ugcagugaaa     1740

aagggauugc cgcacgcgcc ggaauugauc agaagaguca aucgccguau uggcgaacgc     1800

acgucccauc gcguugcgau aucuagagug ggaggaagcu cgcgcagaga guccaucaac     1860

ccauggauuc ugacugguuu cgcugaugcc gaaggaugcu uccgacuaga cauccgcaac     1920

gcaaacucca ugcguguaaa auacagaacu agacuguccu ucgaaaucgu acugcacaac     1980

aaggacaaau cgauucugga gaauauccag ucgacuugga aggucggcaa aaucacaaac     2040

uacggcgaca gaggaguccg ccugagaguc ggccguuucg aagauuugaa agugauuauc     2100

gaccacuucg agaaauaucc gcugauaaca cagaaauugg gcgauuacaa guuguuuaaa     2160

caggcauuca gcgucaugga gaacaaagaa caucuuaagg agaaugggau uaaggagcuc     2220

guacgaauca aagcuaagau gaauuggggu cucaaugacg aauugaaaaa agcauuucca     2280

gagaacauua gcaaagagcg cccccuuauc aauaagaaca uccccaauuu caaauggcug     2340

gcuggauuca caucugguga cggcucguuc augguggaac uaaugaagaa uaagaauaac     2400

guuauuguac gugugcgucu gagauucuca aucucccagc acaucagaga caagaaccug     2460

augaauucau ugauaacaua ccuaggcugu ggucguaucg uugagaauaa caaaucugag     2520

cacaguuggc ucgaauucau uguaacaaaa uucagcgaua ucaacgacaa gaucauuccg     2580

guauuccagg aaaauacucu gauuggcguc aaacucgagg acuuugaaga uuggugcaag     2640

guugccaaau ugaucgaaga gaagaaacac cugaccgaau ccgguuugga ugagauuaag     2700

aaaaucaagc ugaacaugaa caaaggucgu gguggaggcg guagcggagg cggagggucg     2760

gcuagcuccg aagcaccgag agccgaaacc uucguguucc ucgaccugga agccacuggc     2820

cugcccagug uggagcccga gauugccgag cugucccugu ucgccguuca ucgguccucc     2880

cuggaaaacc cugagcacga cgagagcgga gcacuggugc ugccaagggu gcuggacaag     2940

cugacccugu gcaugugucc cgagcguccc uucacugcca aggcauccga aaucacuggu     3000

cugaguucgg aagggcuggc ucguuguaga aaggcuggau ucgauggugc cgugguucgg     3060

acuuugcagg cauuccuguc caggcaggca gguccaaucu gcuuggucgc ccacaacggu     3120

uuugacuacg acuucccacu gcucugugcc gaacugcguc gccugggagc acgccuuccu     3180

cgggauaccg ugugccugga cacccuaccc gcucugcgug gacuggacag agcccacuca     3240

cacggcacua gggcgagagg acgccagggu uacagccucg guucacuguu ccaucgguau     3300

uucagggcag agccuuccgc agcccauuca gccgaaggcg auguccacac cuugcuucug     3360

aucuuccugc aucgugcagc agaacugcuc gccugggcag augagcaggc ucguggaugg     3420

gcucacauug agccgaugua cuugccaccu gaugacccua gccuggaagc cuga           3474


<210>  70
<211>  1157
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity Cb1-b megaTAL-Trex2 protein construct

<400>  70

Met Gly Ser Cys Arg Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            500                 505                 510         


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
        515                 520                 525             


Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser 
    530                 535                 540                 


Ile Val Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr 
545                 550                 555                 560 


Asn Asp His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met 
                565                 570                 575     


Asp Ala Val Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg 
            580                 585                 590         


Val Asn Arg Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser 
        595                 600                 605             


Arg Val Gly Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu 
    610                 615                 620                 


Thr Gly Phe Ala Asp Ala Glu Gly Cys Phe Arg Leu Asp Ile Arg Asn 
625                 630                 635                 640 


Ala Asn Ser Met Arg Val Lys Tyr Arg Thr Arg Leu Ser Phe Glu Ile 
                645                 650                 655     


Val Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr 
            660                 665                 670         


Trp Lys Val Gly Lys Ile Thr Asn Tyr Gly Asp Arg Gly Val Arg Leu 
        675                 680                 685             


Arg Val Gly Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu 
    690                 695                 700                 


Lys Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys 
705                 710                 715                 720 


Gln Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly 
                725                 730                 735     


Ile Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn 
            740                 745                 750         


Asp Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro 
        755                 760                 765             


Leu Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr 
    770                 775                 780                 


Ser Gly Asp Gly Ser Phe Met Val Glu Leu Met Lys Asn Lys Asn Asn 
785                 790                 795                 800 


Val Ile Val Arg Val Arg Leu Arg Phe Ser Ile Ser Gln His Ile Arg 
                805                 810                 815     


Asp Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Arg 
            820                 825                 830         


Ile Val Glu Asn Asn Lys Ser Glu His Ser Trp Leu Glu Phe Ile Val 
        835                 840                 845             


Thr Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu 
    850                 855                 860                 


Asn Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys 
865                 870                 875                 880 


Val Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu 
                885                 890                 895     


Asp Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Gly Gly 
            900                 905                 910         


Gly Gly Ser Gly Gly Gly Gly Ser Ala Ser Ser Glu Ala Pro Arg Ala 
        915                 920                 925             


Glu Thr Phe Val Phe Leu Asp Leu Glu Ala Thr Gly Leu Pro Ser Val 
    930                 935                 940                 


Glu Pro Glu Ile Ala Glu Leu Ser Leu Phe Ala Val His Arg Ser Ser 
945                 950                 955                 960 


Leu Glu Asn Pro Glu His Asp Glu Ser Gly Ala Leu Val Leu Pro Arg 
                965                 970                 975     


Val Leu Asp Lys Leu Thr Leu Cys Met Cys Pro Glu Arg Pro Phe Thr 
            980                 985                 990         


Ala Lys Ala Ser Glu Ile Thr Gly  Leu Ser Ser Glu Gly  Leu Ala Arg 
        995                 1000                 1005             


Cys Arg  Lys Ala Gly Phe Asp  Gly Ala Val Val Arg  Thr Leu Gln 
    1010                 1015                 1020             


Ala Phe  Leu Ser Arg Gln Ala  Gly Pro Ile Cys Leu  Val Ala His 
    1025                 1030                 1035             


Asn Gly  Phe Asp Tyr Asp Phe  Pro Leu Leu Cys Ala  Glu Leu Arg 
    1040                 1045                 1050             


Arg Leu  Gly Ala Arg Leu Pro  Arg Asp Thr Val Cys  Leu Asp Thr 
    1055                 1060                 1065             


Leu Pro  Ala Leu Arg Gly Leu  Asp Arg Ala His Ser  His Gly Thr 
    1070                 1075                 1080             


Arg Ala  Arg Gly Arg Gln Gly  Tyr Ser Leu Gly Ser  Leu Phe His 
    1085                 1090                 1095             


Arg Tyr  Phe Arg Ala Glu Pro  Ser Ala Ala His Ser  Ala Glu Gly 
    1100                 1105                 1110             


Asp Val  His Thr Leu Leu Leu  Ile Phe Leu His Arg  Ala Ala Glu 
    1115                 1120                 1125             


Leu Leu  Ala Trp Ala Asp Glu  Gln Ala Arg Gly Trp  Ala His Ile 
    1130                 1135                 1140             


Glu Pro  Met Tyr Leu Pro Pro  Asp Asp Pro Ser Leu  Glu Ala 
    1145                 1150                 1155         


<210>  71
<211>  3426
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity Cb1-b megaTAL-ExoX DNA construct

<400>  71
atgggatcct gcaggccacc taagaagaaa cgcaaagtcg tggatctacg cacgctcggc       60

tacagtcagc agcagcaaga gaagatcaaa ccgaaggtgc gttcgacagt ggcgcagcac      120

cacgaggcac tggtgggcca tgggtttaca cacgcgcaca tcgttgcgct cagccaacac      180

ccggcagcgt tagggaccgt cgctgtcacg tatcagcaca taatcacggc gttgccagag      240

gcgacacacg aagacatcgt tggcgtcggc aaacagtggt ccggcgcacg cgccctggag      300

gccttgctca cggatgcggg ggagttgaga ggtccgccgt tacagttgga cacaggccaa      360

cttgtgaaga ttgcaaaacg tggcggcgtg accgcaatgg aggcagtgca tgcatcgcgc      420

aatgcactga cgggtgcccc cctgaactta acacccgatc aagtggtagc gatagcgtca      480

aacggcgggg gtaaacaggc tttggagacg gtacagcggt tattgccggt actctgccag      540

gaccacggat tgacaccgga ccaagtggtg gcgattgcgt ccaacaacgg aggcaagcag      600

gcactagaga ctgtccaacg gcttcttccc gttctttgtc aggatcatgg gctaacccct      660

gatcaggtag tcgctatagc ttcaaatgga gggggcaagc aagcactgga gactgttcaa      720

cgactcctgc cagtgctctg ccaagaccac ggacttacac cagatcaagt ggttgctatt      780

gcctccaatg gtggcgggaa acaagcgttg gaaactgtgc agagactgtt acctgtcttg      840

tgtcaagacc acggcctcac gccagatcag gtggtagcca tagcgtcgaa cattggtggt      900

aagcaagccc ttgaaacggt ccagcgtctt ctgccggtgt tgtgccagga ccacggacta      960

acgccggatc aggtcgtagc cattgcttca aatgggggcg gcaaacaggc gctagagaca     1020

gtccagcgcc tcttgcctgt gttatgccag gatcacggct taaccccaga ccaagttgtg     1080

gctattgcat ctaacaatgg tggcaaacaa gccttggaga cagtgcaacg attactgcct     1140

gtcttatgtc aggatcatgg cctgacgccc gatcaggtag tggcaatcgc atctaacatt     1200

ggaggtaagc aagcactgga gactgtccag agattgttac ccgtactatg tcaagatcat     1260

ggtttgacgc ctgatcaggt tgttgcgata gccagcaata acggagggaa acaggctctt     1320

gaaaccgtac agcgacttct cccagtcttg tgccaagatc acgggcttac tcctgatcaa     1380

gtcgtagcta tcgccagcaa taacggtgga aaacaggccc tggaaaccgt acaacgtctc     1440

ctcccagtac tttgtcaaga ccacgggttg actccggatc aagtcgtcgc gatcgcgagc     1500

aatggtgggg ggaagcaggc gctggaaact gttcagagac tgctgcctgt actttgtcag     1560

gaccatggtc tgacacctga ccaagttgtg gcgatagcca gtaatatcgg gggaaaacag     1620

gcactagaga gcattgtggc ccagctgagc cggcctgatc cggcgttggc cgcgttgacc     1680

aacgaccacc tcgtcgcctt ggcctgcctc ggcggacgtc ctgccatgga tgcagtgaaa     1740

aagggattgc cgcacgcgcc ggaattgatc agaagagtca atcgccgtat tggcgaacgc     1800

acgtcccatc gcgttgcgat atctagagtg ggaggaagct cgcgcagaga gtccatcaac     1860

ccatggattc tgactggttt cgctgatgcc gaaggatgct tccgactaga catccgcaac     1920

gcaaactcca tgcgtgtaaa atacagaact agactgtcct tcgaaatcgt actgcacaac     1980

aaggacaaat cgattctgga gaatatccag tcgacttgga aggtcggcaa aatcacaaac     2040

tacggcgaca gaggagtccg cctgagagtc ggccgtttcg aagatttgaa agtgattatc     2100

gaccacttcg agaaatatcc gctgataaca cagaaattgg gcgattacaa gttgtttaaa     2160

caggcattca gcgtcatgga gaacaaagaa catcttaagg agaatgggat taaggagctc     2220

gtacgaatca aagctaagat gaattggggt ctcaatgacg aattgaaaaa agcatttcca     2280

gagaacatta gcaaagagcg cccccttatc aataagaaca tccccaattt caaatggctg     2340

gctggattca catctggtga cggctcgttc atggtggaac taatgaagaa taagaataac     2400

gttattgtac gtgtgcgtct gagattctca atctcccagc acatcagaga caagaacctg     2460

atgaattcat tgataacata cctaggctgt ggtcgtatcg ttgagaataa caaatctgag     2520

cacagttggc tcgaattcat tgtaacaaaa ttcagcgata tcaacgacaa gatcattccg     2580

gtattccagg aaaatactct gattggcgtc aaactcgagg actttgaaga ttggtgcaag     2640

gttgccaaat tgatcgaaga gaagaaacac ctgaccgaat ccggtttgga tgagattaag     2700

aaaatcaagc tgaacatgaa caaaggtcgt ggtggaggcg gtagcggagg cggagggtcg     2760

gctagcctgc ggatcataga cacggaaacc tgtggactcc agggtggtat cgtggagata     2820

gccagtgtcg atgtaattga tgggaaaatc gtaaacccga tgagccacct cgtccgaccg     2880

gataggccga taagtccgca ggctatggct atacatagaa tcacggaggc tatggtagct     2940

gacaagcctt ggatcgagga cgttatccca cactactatg ggagcgaatg gtatgtagcg     3000

cacaacgcat catttgatag gcgcgtgctt cccgaaatgc ctggggagtg gatttgcaca     3060

atgaaactcg ctcgccgact gtggcctggt atcaagtata gtaacatggc actttacaag     3120

acccgcaaac tcaacgtaca gacacctccg ggtctccacc accatagggc actctatgat     3180

tgttacataa ccgctgctct ccttatagac ataatgaata cctccgggtg gaccgccgaa     3240

caaatggcag acataacggg aagacctagt ttgatgacaa cctttacatt tggaaagtat     3300

cggggcaagg cggtgagcga tgtggccgaa agagatcccg gttatcttag atggctcttc     3360

aataacttgg attctatgtc acctgaactt cgccttaccc tgaagcatta ccttgagaat     3420

acctga                                                                3426


<210>  72
<211>  3426
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity Cb1-b megaTAL-ExoX  RNA construct

<400>  72
augggauccu gcaggccacc uaagaagaaa cgcaaagucg uggaucuacg cacgcucggc       60

uacagucagc agcagcaaga gaagaucaaa ccgaaggugc guucgacagu ggcgcagcac      120

cacgaggcac uggugggcca uggguuuaca cacgcgcaca ucguugcgcu cagccaacac      180

ccggcagcgu uagggaccgu cgcugucacg uaucagcaca uaaucacggc guugccagag      240

gcgacacacg aagacaucgu uggcgucggc aaacaguggu ccggcgcacg cgcccuggag      300

gccuugcuca cggaugcggg ggaguugaga gguccgccgu uacaguugga cacaggccaa      360

cuugugaaga uugcaaaacg uggcggcgug accgcaaugg aggcagugca ugcaucgcgc      420

aaugcacuga cgggugcccc ccugaacuua acacccgauc aagugguagc gauagcguca      480

aacggcgggg guaaacaggc uuuggagacg guacagcggu uauugccggu acucugccag      540

gaccacggau ugacaccgga ccaaguggug gcgauugcgu ccaacaacgg aggcaagcag      600

gcacuagaga cuguccaacg gcuucuuccc guucuuuguc aggaucaugg gcuaaccccu      660

gaucagguag ucgcuauagc uucaaaugga gggggcaagc aagcacugga gacuguucaa      720

cgacuccugc cagugcucug ccaagaccac ggacuuacac cagaucaagu gguugcuauu      780

gccuccaaug guggcgggaa acaagcguug gaaacugugc agagacuguu accugucuug      840

ugucaagacc acggccucac gccagaucag gugguagcca uagcgucgaa cauugguggu      900

aagcaagccc uugaaacggu ccagcgucuu cugccggugu ugugccagga ccacggacua      960

acgccggauc aggucguagc cauugcuuca aaugggggcg gcaaacaggc gcuagagaca     1020

guccagcgcc ucuugccugu guuaugccag gaucacggcu uaaccccaga ccaaguugug     1080

gcuauugcau cuaacaaugg uggcaaacaa gccuuggaga cagugcaacg auuacugccu     1140

gucuuauguc aggaucaugg ccugacgccc gaucagguag uggcaaucgc aucuaacauu     1200

ggagguaagc aagcacugga gacuguccag agauuguuac ccguacuaug ucaagaucau     1260

gguuugacgc cugaucaggu uguugcgaua gccagcaaua acggagggaa acaggcucuu     1320

gaaaccguac agcgacuucu cccagucuug ugccaagauc acgggcuuac uccugaucaa     1380

gucguagcua ucgccagcaa uaacggugga aaacaggccc uggaaaccgu acaacgucuc     1440

cucccaguac uuugucaaga ccacggguug acuccggauc aagucgucgc gaucgcgagc     1500

aauggugggg ggaagcaggc gcuggaaacu guucagagac ugcugccugu acuuugucag     1560

gaccaugguc ugacaccuga ccaaguugug gcgauagcca guaauaucgg gggaaaacag     1620

gcacuagaga gcauuguggc ccagcugagc cggccugauc cggcguuggc cgcguugacc     1680

aacgaccacc ucgucgccuu ggccugccuc ggcggacguc cugccaugga ugcagugaaa     1740

aagggauugc cgcacgcgcc ggaauugauc agaagaguca aucgccguau uggcgaacgc     1800

acgucccauc gcguugcgau aucuagagug ggaggaagcu cgcgcagaga guccaucaac     1860

ccauggauuc ugacugguuu cgcugaugcc gaaggaugcu uccgacuaga cauccgcaac     1920

gcaaacucca ugcguguaaa auacagaacu agacuguccu ucgaaaucgu acugcacaac     1980

aaggacaaau cgauucugga gaauauccag ucgacuugga aggucggcaa aaucacaaac     2040

uacggcgaca gaggaguccg ccugagaguc ggccguuucg aagauuugaa agugauuauc     2100

gaccacuucg agaaauaucc gcugauaaca cagaaauugg gcgauuacaa guuguuuaaa     2160

caggcauuca gcgucaugga gaacaaagaa caucuuaagg agaaugggau uaaggagcuc     2220

guacgaauca aagcuaagau gaauuggggu cucaaugacg aauugaaaaa agcauuucca     2280

gagaacauua gcaaagagcg cccccuuauc aauaagaaca uccccaauuu caaauggcug     2340

gcuggauuca caucugguga cggcucguuc augguggaac uaaugaagaa uaagaauaac     2400

guuauuguac gugugcgucu gagauucuca aucucccagc acaucagaga caagaaccug     2460

augaauucau ugauaacaua ccuaggcugu ggucguaucg uugagaauaa caaaucugag     2520

cacaguuggc ucgaauucau uguaacaaaa uucagcgaua ucaacgacaa gaucauuccg     2580

guauuccagg aaaauacucu gauuggcguc aaacucgagg acuuugaaga uuggugcaag     2640

guugccaaau ugaucgaaga gaagaaacac cugaccgaau ccgguuugga ugagauuaag     2700

aaaaucaagc ugaacaugaa caaaggucgu gguggaggcg guagcggagg cggagggucg     2760

gcuagccugc ggaucauaga cacggaaacc uguggacucc agggugguau cguggagaua     2820

gccagugucg auguaauuga ugggaaaauc guaaacccga ugagccaccu cguccgaccg     2880

gauaggccga uaaguccgca ggcuauggcu auacauagaa ucacggaggc uaugguagcu     2940

gacaagccuu ggaucgagga cguuauccca cacuacuaug ggagcgaaug guauguagcg     3000

cacaacgcau cauuugauag gcgcgugcuu cccgaaaugc cuggggagug gauuugcaca     3060

augaaacucg cucgccgacu guggccuggu aucaaguaua guaacauggc acuuuacaag     3120

acccgcaaac ucaacguaca gacaccuccg ggucuccacc accauagggc acucuaugau     3180

uguuacauaa ccgcugcucu ccuuauagac auaaugaaua ccuccgggug gaccgccgaa     3240

caaauggcag acauaacggg aagaccuagu uugaugacaa ccuuuacauu uggaaaguau     3300

cggggcaagg cggugagcga uguggccgaa agagaucccg guuaucuuag auggcucuuc     3360

aauaacuugg auucuauguc accugaacuu cgccuuaccc ugaagcauua ccuugagaau     3420

accuga                                                                3426


<210>  73
<211>  1141
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - low activity Cb1-b megaTAL-ExoX protein construct

<400>  73

Met Gly Ser Cys Arg Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Ile 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            500                 505                 510         


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
        515                 520                 525             


Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Ser 
    530                 535                 540                 


Ile Val Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr 
545                 550                 555                 560 


Asn Asp His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met 
                565                 570                 575     


Asp Ala Val Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg 
            580                 585                 590         


Val Asn Arg Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser 
        595                 600                 605             


Arg Val Gly Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu 
    610                 615                 620                 


Thr Gly Phe Ala Asp Ala Glu Gly Cys Phe Arg Leu Asp Ile Arg Asn 
625                 630                 635                 640 


Ala Asn Ser Met Arg Val Lys Tyr Arg Thr Arg Leu Ser Phe Glu Ile 
                645                 650                 655     


Val Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr 
            660                 665                 670         


Trp Lys Val Gly Lys Ile Thr Asn Tyr Gly Asp Arg Gly Val Arg Leu 
        675                 680                 685             


Arg Val Gly Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu 
    690                 695                 700                 


Lys Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys 
705                 710                 715                 720 


Gln Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly 
                725                 730                 735     


Ile Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn 
            740                 745                 750         


Asp Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro 
        755                 760                 765             


Leu Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr 
    770                 775                 780                 


Ser Gly Asp Gly Ser Phe Met Val Glu Leu Met Lys Asn Lys Asn Asn 
785                 790                 795                 800 


Val Ile Val Arg Val Arg Leu Arg Phe Ser Ile Ser Gln His Ile Arg 
                805                 810                 815     


Asp Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Arg 
            820                 825                 830         


Ile Val Glu Asn Asn Lys Ser Glu His Ser Trp Leu Glu Phe Ile Val 
        835                 840                 845             


Thr Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu 
    850                 855                 860                 


Asn Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys 
865                 870                 875                 880 


Val Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu 
                885                 890                 895     


Asp Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Gly Gly 
            900                 905                 910         


Gly Gly Ser Gly Gly Gly Gly Ser Ala Ser Leu Arg Ile Ile Asp Thr 
        915                 920                 925             


Glu Thr Cys Gly Leu Gln Gly Gly Ile Val Glu Ile Ala Ser Val Asp 
    930                 935                 940                 


Val Ile Asp Gly Lys Ile Val Asn Pro Met Ser His Leu Val Arg Pro 
945                 950                 955                 960 


Asp Arg Pro Ile Ser Pro Gln Ala Met Ala Ile His Arg Ile Thr Glu 
                965                 970                 975     


Ala Met Val Ala Asp Lys Pro Trp Ile Glu Asp Val Ile Pro His Tyr 
            980                 985                 990         


Tyr Gly Ser Glu Trp Tyr Val Ala  His Asn Ala Ser Phe  Asp Arg Arg 
        995                 1000                 1005             


Val Leu  Pro Glu Met Pro Gly  Glu Trp Ile Cys Thr  Met Lys Leu 
    1010                 1015                 1020             


Ala Arg  Arg Leu Trp Pro Gly  Ile Lys Tyr Ser Asn  Met Ala Leu 
    1025                 1030                 1035             


Tyr Lys  Thr Arg Lys Leu Asn  Val Gln Thr Pro Pro  Gly Leu His 
    1040                 1045                 1050             


His His  Arg Ala Leu Tyr Asp  Cys Tyr Ile Thr Ala  Ala Leu Leu 
    1055                 1060                 1065             


Ile Asp  Ile Met Asn Thr Ser  Gly Trp Thr Ala Glu  Gln Met Ala 
    1070                 1075                 1080             


Asp Ile  Thr Gly Arg Pro Ser  Leu Met Thr Thr Phe  Thr Phe Gly 
    1085                 1090                 1095             


Lys Tyr  Arg Gly Lys Ala Val  Ser Asp Val Ala Glu  Arg Asp Pro 
    1100                 1105                 1110             


Gly Tyr  Leu Arg Trp Leu Phe  Asn Asn Leu Asp Ser  Met Ser Pro 
    1115                 1120                 1125             


Glu Leu  Arg Leu Thr Leu Lys  His Tyr Leu Glu Asn  Thr 
    1130                 1135                 1140     


<210>  74
<211>  2835
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - PD-1 megaTAL DNA construct

<400>  74
atgggatcct gcaggccacc taagaagaaa cgcaaagtcg tggatctacg cacgctcggc       60

tacagtcagc agcagcaaga gaagatcaaa ccgaaggtgc gttcgacagt ggcgcagcac      120

cacgaggcac tggtgggcca tgggtttaca cacgcgcaca tcgttgcgct cagccaacac      180

ccggcagcgt tagggaccgt cgctgtcacg tatcagcaca taatcacggc gttgccagag      240

gcgacacacg aagacatcgt tggcgtcggc aaacagtggt ccggcgcacg cgccctggag      300

gccttgctca cggatgcggg ggagttgaga ggtccgccgt tacagttgga cacaggccaa      360

cttgtgaaga ttgcaaaacg tggcggcgtg accgcaatgg aggcagtgca tgcatcgcgc      420

aatgcactga cgggtgcccc cctgaaccta acccctgatc aggtagtcgc tatagcttca      480

aacaacgggg gcaagcaagc actggagacc gttcaacgac tcctgccagt gctctgccaa      540

gaccacggac ttacgccaga tcaggtggtt gctattgcct ccaacaatgg cgggaaacaa      600

gcgttggaaa ctgtgcagag actgttacct gtcttgtgtc aagaccacgg cctcacgcca      660

gatcaggtgg tagccatagc gtcgaatgga ggtggtaagc aagcccttga aacggtccag      720

cgtcttctgc cggtgttgtg ccaggaccac ggactaacgc cggatcaggt cgtagccatt      780

gcttcaaata acggcggcaa acaggcgcta gagacagtcc agcgcctctt gcctgtgtta      840

tgccaggatc acggcttaac cccagaccaa gttgtggcta ttgcatctaa caatggtggc      900

aaacaagcct tggagacagt gcaacgatta ctgcctgtct tatgtcagga tcatggcctg      960

acgcccgatc aggtagtggc aatcgcatct aataatggag gtaagcaagc actggagact     1020

gtccagagat tgttacccgt actatgtcaa gatcatggtt tgacgcctga tcaggttgtt     1080

gcgatagcca gcaacaacgg agggaaacag gctcttgaaa ccgtacagcg acttctccca     1140

gtcttgtgcc aagatcacgg gcttactcct gatcaagtcg tagctatcgc cagccacgac     1200

ggtgggaaac aggccctgga aaccgtacaa cgtctcctcc cagtactttg tcaagaccac     1260

gggttgactc cggatcaagt cgtcgcgatc gcgagcaatg gaggggggaa gcaggcgctg     1320

gaaactgttc agagactgct gcctgtactt tgtcaggacc atggtctgac acctgaccaa     1380

gttgtggcga tagccagtaa caatggggga aaacaggcac tagagacggt tcaaaggttg     1440

ttgcccgttc tgtgccagga ccacggcttg acaccggatc aggtggtagc tatcgcttca     1500

cacgatggcg gaaaacaggc tttagaaaca gtccaaagac ttctcccagt cctttgtcag     1560

gaccacggat tgactccaga tcaagtcgtt gctattgcaa gtaatggtgg tggtaagcaa     1620

gctttagaaa ccgtacagag gcttttgcca gtgctgtgcc aggaccatgg actgacccct     1680

gatcaagtgg tagcaattgc atctcatgat ggaggaaaac aagctctgga aagcattgtg     1740

gcccagctga gccggcctga tccggcgttg gccgcgttga ccaacgacca cctcgtcgcc     1800

ttggcctgcc tcggcggacg tcctgccatg gatgcagtga aaaagggatt gccgcacgcg     1860

ccggaattga tcagaagagt caatcgccgt attggcgaac gcacgtccca tcgcgttgcg     1920

atatctagag tgggaggaag ctctcgcaga gagtccatca acccatggac tctgactggt     1980

ttcgctgatg ccgaaggatc attcgggcta agcatcctca acagaaacag aggtactgct     2040

agataccaca ctcgactgtc attcacaatc atgctgcaca acaaggacaa atcgattctg     2100

gagaatatcc agtcgacttg gaaggtcggc agcatcctca acaatggcga ccactacgtc     2160

tcgctggtgg tctaccgttt cgaagatttg aaagtgatta tcgaccactt cgagaaatat     2220

ccgctgataa cacagaaatt gggcgattac aagttgttta aacaggcatt cagcgtcatg     2280

gagaacaaag aacatcttaa ggagaatggg attaaggagc tcgtacgaat caaagctaag     2340

atgaattggg gtctcaatga cgaattgaaa aaagcatttc cagaggtgat tagcagggag     2400

cgccccctta tcaataagaa cattccgaat gggaaatggc tggctggatt cacatctggt     2460

gatggctcct tcttcgtgcg cctaagaaag tctaatgtta atgctagagt acgtgtgcaa     2520

ctggtattcg agatctcaca gcacatcaga gacaagaacc tgatgaattc attgataaca     2580

tacctaggct gtggtcacat ctacgaggga aacaaatctg agcgcagttg gctccaattc     2640

agagtagaaa aattcagcga tatcaacgac aagatcattc cggtattcca ggaaaatact     2700

ctgattggca tgaaactcga ggactttgaa gattggtgca aggttgccaa attgatcgaa     2760

gagaagaaac acctgaccga atccggtttg gatgagatta agaaaatcaa gctgaacatg     2820

aacaaaagac gttga                                                      2835


<210>  75
<211>  2835
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - PD-1  megaTAL RNA construct

<400>  75
augggauccu gcaggccacc uaagaagaaa cgcaaagucg uggaucuacg cacgcucggc       60

uacagucagc agcagcaaga gaagaucaaa ccgaaggugc guucgacagu ggcgcagcac      120

cacgaggcac uggugggcca uggguuuaca cacgcgcaca ucguugcgcu cagccaacac      180

ccggcagcgu uagggaccgu cgcugucacg uaucagcaca uaaucacggc guugccagag      240

gcgacacacg aagacaucgu uggcgucggc aaacaguggu ccggcgcacg cgcccuggag      300

gccuugcuca cggaugcggg ggaguugaga gguccgccgu uacaguugga cacaggccaa      360

cuugugaaga uugcaaaacg uggcggcgug accgcaaugg aggcagugca ugcaucgcgc      420

aaugcacuga cgggugcccc ccugaaccua accccugauc agguagucgc uauagcuuca      480

aacaacgggg gcaagcaagc acuggagacc guucaacgac uccugccagu gcucugccaa      540

gaccacggac uuacgccaga ucaggugguu gcuauugccu ccaacaaugg cgggaaacaa      600

gcguuggaaa cugugcagag acuguuaccu gucuuguguc aagaccacgg ccucacgcca      660

gaucaggugg uagccauagc gucgaaugga ggugguaagc aagcccuuga aacgguccag      720

cgucuucugc cgguguugug ccaggaccac ggacuaacgc cggaucaggu cguagccauu      780

gcuucaaaua acggcggcaa acaggcgcua gagacagucc agcgccucuu gccuguguua      840

ugccaggauc acggcuuaac cccagaccaa guuguggcua uugcaucuaa caaugguggc      900

aaacaagccu uggagacagu gcaacgauua cugccugucu uaugucagga ucauggccug      960

acgcccgauc agguaguggc aaucgcaucu aauaauggag guaagcaagc acuggagacu     1020

guccagagau uguuacccgu acuaugucaa gaucaugguu ugacgccuga ucagguuguu     1080

gcgauagcca gcaacaacgg agggaaacag gcucuugaaa ccguacagcg acuucuccca     1140

gucuugugcc aagaucacgg gcuuacuccu gaucaagucg uagcuaucgc cagccacgac     1200

ggugggaaac aggcccugga aaccguacaa cgucuccucc caguacuuug ucaagaccac     1260

ggguugacuc cggaucaagu cgucgcgauc gcgagcaaug gaggggggaa gcaggcgcug     1320

gaaacuguuc agagacugcu gccuguacuu ugucaggacc auggucugac accugaccaa     1380

guuguggcga uagccaguaa caauggggga aaacaggcac uagagacggu ucaaagguug     1440

uugcccguuc ugugccagga ccacggcuug acaccggauc aggugguagc uaucgcuuca     1500

cacgauggcg gaaaacaggc uuuagaaaca guccaaagac uucucccagu ccuuugucag     1560

gaccacggau ugacuccaga ucaagucguu gcuauugcaa guaauggugg ugguaagcaa     1620

gcuuuagaaa ccguacagag gcuuuugcca gugcugugcc aggaccaugg acugaccccu     1680

gaucaagugg uagcaauugc aucucaugau ggaggaaaac aagcucugga aagcauugug     1740

gcccagcuga gccggccuga uccggcguug gccgcguuga ccaacgacca ccucgucgcc     1800

uuggccugcc ucggcggacg uccugccaug gaugcaguga aaaagggauu gccgcacgcg     1860

ccggaauuga ucagaagagu caaucgccgu auuggcgaac gcacguccca ucgcguugcg     1920

auaucuagag ugggaggaag cucucgcaga gaguccauca acccauggac ucugacuggu     1980

uucgcugaug ccgaaggauc auucgggcua agcauccuca acagaaacag agguacugcu     2040

agauaccaca cucgacuguc auucacaauc augcugcaca acaaggacaa aucgauucug     2100

gagaauaucc agucgacuug gaaggucggc agcauccuca acaauggcga ccacuacguc     2160

ucgcuggugg ucuaccguuu cgaagauuug aaagugauua ucgaccacuu cgagaaauau     2220

ccgcugauaa cacagaaauu gggcgauuac aaguuguuua aacaggcauu cagcgucaug     2280

gagaacaaag aacaucuuaa ggagaauggg auuaaggagc ucguacgaau caaagcuaag     2340

augaauuggg gucucaauga cgaauugaaa aaagcauuuc cagaggugau uagcagggag     2400

cgcccccuua ucaauaagaa cauuccgaau gggaaauggc uggcuggauu cacaucuggu     2460

gauggcuccu ucuucgugcg ccuaagaaag ucuaauguua augcuagagu acgugugcaa     2520

cugguauucg agaucucaca gcacaucaga gacaagaacc ugaugaauuc auugauaaca     2580

uaccuaggcu guggucacau cuacgaggga aacaaaucug agcgcaguug gcuccaauuc     2640

agaguagaaa aauucagcga uaucaacgac aagaucauuc cgguauucca ggaaaauacu     2700

cugauuggca ugaaacucga ggacuuugaa gauuggugca agguugccaa auugaucgaa     2760

gagaagaaac accugaccga auccgguuug gaugagauua agaaaaucaa gcugaacaug     2820

aacaaaagac guuga                                                      2835


<210>  76
<211>  944
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - PD-1  megaTAL protein construct

<400>  76

Met Gly Ser Cys Arg Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser His Asp 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            500                 505                 510         


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
        515                 520                 525             


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
    530                 535                 540                 


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
545                 550                 555                 560 


Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu 
                565                 570                 575     


Glu Ser Ile Val Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala 
            580                 585                 590         


Leu Thr Asn Asp His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro 
        595                 600                 605             


Ala Met Asp Ala Val Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile 
    610                 615                 620                 


Arg Arg Val Asn Arg Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala 
625                 630                 635                 640 


Ile Ser Arg Val Gly Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp 
                645                 650                 655     


Thr Leu Thr Gly Phe Ala Asp Ala Glu Gly Ser Phe Gly Leu Ser Ile 
            660                 665                 670         


Leu Asn Arg Asn Arg Gly Thr Ala Arg Tyr His Thr Arg Leu Ser Phe 
        675                 680                 685             


Thr Ile Met Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln 
    690                 695                 700                 


Ser Thr Trp Lys Val Gly Ser Ile Leu Asn Asn Gly Asp His Tyr Val 
705                 710                 715                 720 


Ser Leu Val Val Tyr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His 
                725                 730                 735     


Phe Glu Lys Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu 
            740                 745                 750         


Phe Lys Gln Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu 
        755                 760                 765             


Asn Gly Ile Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly 
    770                 775                 780                 


Leu Asn Asp Glu Leu Lys Lys Ala Phe Pro Glu Val Ile Ser Arg Glu 
785                 790                 795                 800 


Arg Pro Leu Ile Asn Lys Asn Ile Pro Asn Gly Lys Trp Leu Ala Gly 
                805                 810                 815     


Phe Thr Ser Gly Asp Gly Ser Phe Phe Val Arg Leu Arg Lys Ser Asn 
            820                 825                 830         


Val Asn Ala Arg Val Arg Val Gln Leu Val Phe Glu Ile Ser Gln His 
        835                 840                 845             


Ile Arg Asp Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys 
    850                 855                 860                 


Gly His Ile Tyr Glu Gly Asn Lys Ser Glu Arg Ser Trp Leu Gln Phe 
865                 870                 875                 880 


Arg Val Glu Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe 
                885                 890                 895     


Gln Glu Asn Thr Leu Ile Gly Met Lys Leu Glu Asp Phe Glu Asp Trp 
            900                 905                 910         


Cys Lys Val Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser 
        915                 920                 925             


Gly Leu Asp Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Arg Arg 
    930                 935                 940                 


<210>  77
<211>  3576
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - PD-1 megaTAL-Trex2 DNA construct

<400>  77
atgggatcct gcaggccacc taagaagaaa cgcaaagtcg tggatctacg cacgctcggc       60

tacagtcagc agcagcaaga gaagatcaaa ccgaaggtgc gttcgacagt ggcgcagcac      120

cacgaggcac tggtgggcca tgggtttaca cacgcgcaca tcgttgcgct cagccaacac      180

ccggcagcgt tagggaccgt cgctgtcacg tatcagcaca taatcacggc gttgccagag      240

gcgacacacg aagacatcgt tggcgtcggc aaacagtggt ccggcgcacg cgccctggag      300

gccttgctca cggatgcggg ggagttgaga ggtccgccgt tacagttgga cacaggccaa      360

cttgtgaaga ttgcaaaacg tggcggcgtg accgcaatgg aggcagtgca tgcatcgcgc      420

aatgcactga cgggtgcccc cctgaaccta acccctgatc aggtagtcgc tatagcttca      480

aacaacgggg gcaagcaagc actggagacc gttcaacgac tcctgccagt gctctgccaa      540

gaccacggac ttacgccaga tcaggtggtt gctattgcct ccaacaatgg cgggaaacaa      600

gcgttggaaa ctgtgcagag actgttacct gtcttgtgtc aagaccacgg cctcacgcca      660

gatcaggtgg tagccatagc gtcgaatgga ggtggtaagc aagcccttga aacggtccag      720

cgtcttctgc cggtgttgtg ccaggaccac ggactaacgc cggatcaggt cgtagccatt      780

gcttcaaata acggcggcaa acaggcgcta gagacagtcc agcgcctctt gcctgtgtta      840

tgccaggatc acggcttaac cccagaccaa gttgtggcta ttgcatctaa caatggtggc      900

aaacaagcct tggagacagt gcaacgatta ctgcctgtct tatgtcagga tcatggcctg      960

acgcccgatc aggtagtggc aatcgcatct aataatggag gtaagcaagc actggagact     1020

gtccagagat tgttacccgt actatgtcaa gatcatggtt tgacgcctga tcaggttgtt     1080

gcgatagcca gcaacaacgg agggaaacag gctcttgaaa ccgtacagcg acttctccca     1140

gtcttgtgcc aagatcacgg gcttactcct gatcaagtcg tagctatcgc cagccacgac     1200

ggtgggaaac aggccctgga aaccgtacaa cgtctcctcc cagtactttg tcaagaccac     1260

gggttgactc cggatcaagt cgtcgcgatc gcgagcaatg gaggggggaa gcaggcgctg     1320

gaaactgttc agagactgct gcctgtactt tgtcaggacc atggtctgac acctgaccaa     1380

gttgtggcga tagccagtaa caatggggga aaacaggcac tagagacggt tcaaaggttg     1440

ttgcccgttc tgtgccagga ccacggcttg acaccggatc aggtggtagc tatcgcttca     1500

cacgatggcg gaaaacaggc tttagaaaca gtccaaagac ttctcccagt cctttgtcag     1560

gaccacggat tgactccaga tcaagtcgtt gctattgcaa gtaatggtgg tggtaagcaa     1620

gctttagaaa ccgtacagag gcttttgcca gtgctgtgcc aggaccatgg actgacccct     1680

gatcaagtgg tagcaattgc atctcatgat ggaggaaaac aagctctgga aagcattgtg     1740

gcccagctga gccggcctga tccggcgttg gccgcgttga ccaacgacca cctcgtcgcc     1800

ttggcctgcc tcggcggacg tcctgccatg gatgcagtga aaaagggatt gccgcacgcg     1860

ccggaattga tcagaagagt caatcgccgt attggcgaac gcacgtccca tcgcgttgcg     1920

atatctagag tgggaggaag ctctcgcaga gagtccatca acccatggac tctgactggt     1980

ttcgctgatg ccgaaggatc attcgggcta agcatcctca acagaaacag aggtactgct     2040

agataccaca ctcgactgtc attcacaatc atgctgcaca acaaggacaa atcgattctg     2100

gagaatatcc agtcgacttg gaaggtcggc agcatcctca acaatggcga ccactacgtc     2160

tcgctggtgg tctaccgttt cgaagatttg aaagtgatta tcgaccactt cgagaaatat     2220

ccgctgataa cacagaaatt gggcgattac aagttgttta aacaggcatt cagcgtcatg     2280

gagaacaaag aacatcttaa ggagaatggg attaaggagc tcgtacgaat caaagctaag     2340

atgaattggg gtctcaatga cgaattgaaa aaagcatttc cagaggtgat tagcagggag     2400

cgccccctta tcaataagaa cattccgaat gggaaatggc tggctggatt cacatctggt     2460

gatggctcct tcttcgtgcg cctaagaaag tctaatgtta atgctagagt acgtgtgcaa     2520

ctggtattcg agatctcaca gcacatcaga gacaagaacc tgatgaattc attgataaca     2580

tacctaggct gtggtcacat ctacgaggga aacaaatctg agcgcagttg gctccaattc     2640

agagtagaaa aattcagcga tatcaacgac aagatcattc cggtattcca ggaaaatact     2700

ctgattggca tgaaactcga ggactttgaa gattggtgca aggttgccaa attgatcgaa     2760

gagaagaaac acctgaccga atccggtttg gatgagatta agaaaatcaa gctgaacatg     2820

aacaaaagac gtggtggagg cggtagcgga ggcggagggt cggctagctc cgaagcaccg     2880

agagccgaaa ccttcgtgtt cctcgacctg gaagccactg gcctgcccag tgtggagccc     2940

gagattgccg agctgtccct gttcgccgtt catcggtcct ccctggaaaa ccctgagcac     3000

gacgagagcg gagcactggt gctgccaagg gtgctggaca agctgaccct gtgcatgtgt     3060

cccgagcgtc ccttcactgc caaggcatcc gaaatcactg gtctgagttc ggaagggctg     3120

gctcgttgta gaaaggctgg attcgatggt gccgtggttc ggactttgca ggcattcctg     3180

tccaggcagg caggtccaat ctgcttggtc gcccacaacg gttttgacta cgacttccca     3240

ctgctctgtg ccgaactgcg tcgcctggga gcacgccttc ctcgggatac cgtgtgcctg     3300

gacaccctac ccgctctgcg tggactggac agagcccact cacacggcac tagggcgaga     3360

ggacgccagg gttacagcct cggttcactg ttccatcggt atttcagggc agagccttcc     3420

gcagcccatt cagccgaagg cgatgtccac accttgcttc tgatcttcct gcatcgtgca     3480

gcagaactgc tcgcctgggc agatgagcag gctcgtggat gggctcacat tgagccgatg     3540

tacttgccac ctgatgaccc tagcctggaa gcctga                               3576


<210>  78
<211>  3576
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - PD-1 megaTAL-Trex2 RNA construct

<400>  78
augggauccu gcaggccacc uaagaagaaa cgcaaagucg uggaucuacg cacgcucggc       60

uacagucagc agcagcaaga gaagaucaaa ccgaaggugc guucgacagu ggcgcagcac      120

cacgaggcac uggugggcca uggguuuaca cacgcgcaca ucguugcgcu cagccaacac      180

ccggcagcgu uagggaccgu cgcugucacg uaucagcaca uaaucacggc guugccagag      240

gcgacacacg aagacaucgu uggcgucggc aaacaguggu ccggcgcacg cgcccuggag      300

gccuugcuca cggaugcggg ggaguugaga gguccgccgu uacaguugga cacaggccaa      360

cuugugaaga uugcaaaacg uggcggcgug accgcaaugg aggcagugca ugcaucgcgc      420

aaugcacuga cgggugcccc ccugaaccua accccugauc agguagucgc uauagcuuca      480

aacaacgggg gcaagcaagc acuggagacc guucaacgac uccugccagu gcucugccaa      540

gaccacggac uuacgccaga ucaggugguu gcuauugccu ccaacaaugg cgggaaacaa      600

gcguuggaaa cugugcagag acuguuaccu gucuuguguc aagaccacgg ccucacgcca      660

gaucaggugg uagccauagc gucgaaugga ggugguaagc aagcccuuga aacgguccag      720

cgucuucugc cgguguugug ccaggaccac ggacuaacgc cggaucaggu cguagccauu      780

gcuucaaaua acggcggcaa acaggcgcua gagacagucc agcgccucuu gccuguguua      840

ugccaggauc acggcuuaac cccagaccaa guuguggcua uugcaucuaa caaugguggc      900

aaacaagccu uggagacagu gcaacgauua cugccugucu uaugucagga ucauggccug      960

acgcccgauc agguaguggc aaucgcaucu aauaauggag guaagcaagc acuggagacu     1020

guccagagau uguuacccgu acuaugucaa gaucaugguu ugacgccuga ucagguuguu     1080

gcgauagcca gcaacaacgg agggaaacag gcucuugaaa ccguacagcg acuucuccca     1140

gucuugugcc aagaucacgg gcuuacuccu gaucaagucg uagcuaucgc cagccacgac     1200

ggugggaaac aggcccugga aaccguacaa cgucuccucc caguacuuug ucaagaccac     1260

ggguugacuc cggaucaagu cgucgcgauc gcgagcaaug gaggggggaa gcaggcgcug     1320

gaaacuguuc agagacugcu gccuguacuu ugucaggacc auggucugac accugaccaa     1380

guuguggcga uagccaguaa caauggggga aaacaggcac uagagacggu ucaaagguug     1440

uugcccguuc ugugccagga ccacggcuug acaccggauc aggugguagc uaucgcuuca     1500

cacgauggcg gaaaacaggc uuuagaaaca guccaaagac uucucccagu ccuuugucag     1560

gaccacggau ugacuccaga ucaagucguu gcuauugcaa guaauggugg ugguaagcaa     1620

gcuuuagaaa ccguacagag gcuuuugcca gugcugugcc aggaccaugg acugaccccu     1680

gaucaagugg uagcaauugc aucucaugau ggaggaaaac aagcucugga aagcauugug     1740

gcccagcuga gccggccuga uccggcguug gccgcguuga ccaacgacca ccucgucgcc     1800

uuggccugcc ucggcggacg uccugccaug gaugcaguga aaaagggauu gccgcacgcg     1860

ccggaauuga ucagaagagu caaucgccgu auuggcgaac gcacguccca ucgcguugcg     1920

auaucuagag ugggaggaag cucucgcaga gaguccauca acccauggac ucugacuggu     1980

uucgcugaug ccgaaggauc auucgggcua agcauccuca acagaaacag agguacugcu     2040

agauaccaca cucgacuguc auucacaauc augcugcaca acaaggacaa aucgauucug     2100

gagaauaucc agucgacuug gaaggucggc agcauccuca acaauggcga ccacuacguc     2160

ucgcuggugg ucuaccguuu cgaagauuug aaagugauua ucgaccacuu cgagaaauau     2220

ccgcugauaa cacagaaauu gggcgauuac aaguuguuua aacaggcauu cagcgucaug     2280

gagaacaaag aacaucuuaa ggagaauggg auuaaggagc ucguacgaau caaagcuaag     2340

augaauuggg gucucaauga cgaauugaaa aaagcauuuc cagaggugau uagcagggag     2400

cgcccccuua ucaauaagaa cauuccgaau gggaaauggc uggcuggauu cacaucuggu     2460

gauggcuccu ucuucgugcg ccuaagaaag ucuaauguua augcuagagu acgugugcaa     2520

cugguauucg agaucucaca gcacaucaga gacaagaacc ugaugaauuc auugauaaca     2580

uaccuaggcu guggucacau cuacgaggga aacaaaucug agcgcaguug gcuccaauuc     2640

agaguagaaa aauucagcga uaucaacgac aagaucauuc cgguauucca ggaaaauacu     2700

cugauuggca ugaaacucga ggacuuugaa gauuggugca agguugccaa auugaucgaa     2760

gagaagaaac accugaccga auccgguuug gaugagauua agaaaaucaa gcugaacaug     2820

aacaaaagac gugguggagg cgguagcgga ggcggagggu cggcuagcuc cgaagcaccg     2880

agagccgaaa ccuucguguu ccucgaccug gaagccacug gccugcccag uguggagccc     2940

gagauugccg agcugucccu guucgccguu caucgguccu cccuggaaaa cccugagcac     3000

gacgagagcg gagcacuggu gcugccaagg gugcuggaca agcugacccu gugcaugugu     3060

cccgagcguc ccuucacugc caaggcaucc gaaaucacug gucugaguuc ggaagggcug     3120

gcucguugua gaaaggcugg auucgauggu gccgugguuc ggacuuugca ggcauuccug     3180

uccaggcagg cagguccaau cugcuugguc gcccacaacg guuuugacua cgacuuccca     3240

cugcucugug ccgaacugcg ucgccuggga gcacgccuuc cucgggauac cgugugccug     3300

gacacccuac ccgcucugcg uggacuggac agagcccacu cacacggcac uagggcgaga     3360

ggacgccagg guuacagccu cgguucacug uuccaucggu auuucagggc agagccuucc     3420

gcagcccauu cagccgaagg cgauguccac accuugcuuc ugaucuuccu gcaucgugca     3480

gcagaacugc ucgccugggc agaugagcag gcucguggau gggcucacau ugagccgaug     3540

uacuugccac cugaugaccc uagccuggaa gccuga                               3576


<210>  79
<211>  1191
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - PD-1 megaTAL-Trex2 protein construct

<400>  79

Met Gly Ser Cys Arg Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser His Asp 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            500                 505                 510         


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
        515                 520                 525             


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
    530                 535                 540                 


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
545                 550                 555                 560 


Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu 
                565                 570                 575     


Glu Ser Ile Val Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala 
            580                 585                 590         


Leu Thr Asn Asp His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro 
        595                 600                 605             


Ala Met Asp Ala Val Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile 
    610                 615                 620                 


Arg Arg Val Asn Arg Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala 
625                 630                 635                 640 


Ile Ser Arg Val Gly Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp 
                645                 650                 655     


Thr Leu Thr Gly Phe Ala Asp Ala Glu Gly Ser Phe Gly Leu Ser Ile 
            660                 665                 670         


Leu Asn Arg Asn Arg Gly Thr Ala Arg Tyr His Thr Arg Leu Ser Phe 
        675                 680                 685             


Thr Ile Met Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln 
    690                 695                 700                 


Ser Thr Trp Lys Val Gly Ser Ile Leu Asn Asn Gly Asp His Tyr Val 
705                 710                 715                 720 


Ser Leu Val Val Tyr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His 
                725                 730                 735     


Phe Glu Lys Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu 
            740                 745                 750         


Phe Lys Gln Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu 
        755                 760                 765             


Asn Gly Ile Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly 
    770                 775                 780                 


Leu Asn Asp Glu Leu Lys Lys Ala Phe Pro Glu Val Ile Ser Arg Glu 
785                 790                 795                 800 


Arg Pro Leu Ile Asn Lys Asn Ile Pro Asn Gly Lys Trp Leu Ala Gly 
                805                 810                 815     


Phe Thr Ser Gly Asp Gly Ser Phe Phe Val Arg Leu Arg Lys Ser Asn 
            820                 825                 830         


Val Asn Ala Arg Val Arg Val Gln Leu Val Phe Glu Ile Ser Gln His 
        835                 840                 845             


Ile Arg Asp Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys 
    850                 855                 860                 


Gly His Ile Tyr Glu Gly Asn Lys Ser Glu Arg Ser Trp Leu Gln Phe 
865                 870                 875                 880 


Arg Val Glu Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe 
                885                 890                 895     


Gln Glu Asn Thr Leu Ile Gly Met Lys Leu Glu Asp Phe Glu Asp Trp 
            900                 905                 910         


Cys Lys Val Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser 
        915                 920                 925             


Gly Leu Asp Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Arg Arg 
    930                 935                 940                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Ser Ser Glu Ala Pro 
945                 950                 955                 960 


Arg Ala Glu Thr Phe Val Phe Leu Asp Leu Glu Ala Thr Gly Leu Pro 
                965                 970                 975     


Ser Val Glu Pro Glu Ile Ala Glu Leu Ser Leu Phe Ala Val His Arg 
            980                 985                 990         


Ser Ser Leu Glu Asn Pro Glu His  Asp Glu Ser Gly Ala  Leu Val Leu 
        995                 1000                 1005             


Pro Arg  Val Leu Asp Lys Leu  Thr Leu Cys Met Cys  Pro Glu Arg 
    1010                 1015                 1020             


Pro Phe  Thr Ala Lys Ala Ser  Glu Ile Thr Gly Leu  Ser Ser Glu 
    1025                 1030                 1035             


Gly Leu  Ala Arg Cys Arg Lys  Ala Gly Phe Asp Gly  Ala Val Val 
    1040                 1045                 1050             


Arg Thr  Leu Gln Ala Phe Leu  Ser Arg Gln Ala Gly  Pro Ile Cys 
    1055                 1060                 1065             


Leu Val  Ala His Asn Gly Phe  Asp Tyr Asp Phe Pro  Leu Leu Cys 
    1070                 1075                 1080             


Ala Glu  Leu Arg Arg Leu Gly  Ala Arg Leu Pro Arg  Asp Thr Val 
    1085                 1090                 1095             


Cys Leu  Asp Thr Leu Pro Ala  Leu Arg Gly Leu Asp  Arg Ala His 
    1100                 1105                 1110             


Ser His  Gly Thr Arg Ala Arg  Gly Arg Gln Gly Tyr  Ser Leu Gly 
    1115                 1120                 1125             


Ser Leu  Phe His Arg Tyr Phe  Arg Ala Glu Pro Ser  Ala Ala His 
    1130                 1135                 1140             


Ser Ala  Glu Gly Asp Val His  Thr Leu Leu Leu Ile  Phe Leu His 
    1145                 1150                 1155             


Arg Ala  Ala Glu Leu Leu Ala  Trp Ala Asp Glu Gln  Ala Arg Gly 
    1160                 1165                 1170             


Trp Ala  His Ile Glu Pro Met  Tyr Leu Pro Pro Asp  Asp Pro Ser 
    1175                 1180                 1185             


Leu Glu  Ala 
    1190     


<210>  80
<211>  3528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - PD-1 megaTAL-ExoX DNA construct

<400>  80
atgggatcct gcaggccacc taagaagaaa cgcaaagtcg tggatctacg cacgctcggc       60

tacagtcagc agcagcaaga gaagatcaaa ccgaaggtgc gttcgacagt ggcgcagcac      120

cacgaggcac tggtgggcca tgggtttaca cacgcgcaca tcgttgcgct cagccaacac      180

ccggcagcgt tagggaccgt cgctgtcacg tatcagcaca taatcacggc gttgccagag      240

gcgacacacg aagacatcgt tggcgtcggc aaacagtggt ccggcgcacg cgccctggag      300

gccttgctca cggatgcggg ggagttgaga ggtccgccgt tacagttgga cacaggccaa      360

cttgtgaaga ttgcaaaacg tggcggcgtg accgcaatgg aggcagtgca tgcatcgcgc      420

aatgcactga cgggtgcccc cctgaaccta acccctgatc aggtagtcgc tatagcttca      480

aacaacgggg gcaagcaagc actggagacc gttcaacgac tcctgccagt gctctgccaa      540

gaccacggac ttacgccaga tcaggtggtt gctattgcct ccaacaatgg cgggaaacaa      600

gcgttggaaa ctgtgcagag actgttacct gtcttgtgtc aagaccacgg cctcacgcca      660

gatcaggtgg tagccatagc gtcgaatgga ggtggtaagc aagcccttga aacggtccag      720

cgtcttctgc cggtgttgtg ccaggaccac ggactaacgc cggatcaggt cgtagccatt      780

gcttcaaata acggcggcaa acaggcgcta gagacagtcc agcgcctctt gcctgtgtta      840

tgccaggatc acggcttaac cccagaccaa gttgtggcta ttgcatctaa caatggtggc      900

aaacaagcct tggagacagt gcaacgatta ctgcctgtct tatgtcagga tcatggcctg      960

acgcccgatc aggtagtggc aatcgcatct aataatggag gtaagcaagc actggagact     1020

gtccagagat tgttacccgt actatgtcaa gatcatggtt tgacgcctga tcaggttgtt     1080

gcgatagcca gcaacaacgg agggaaacag gctcttgaaa ccgtacagcg acttctccca     1140

gtcttgtgcc aagatcacgg gcttactcct gatcaagtcg tagctatcgc cagccacgac     1200

ggtgggaaac aggccctgga aaccgtacaa cgtctcctcc cagtactttg tcaagaccac     1260

gggttgactc cggatcaagt cgtcgcgatc gcgagcaatg gaggggggaa gcaggcgctg     1320

gaaactgttc agagactgct gcctgtactt tgtcaggacc atggtctgac acctgaccaa     1380

gttgtggcga tagccagtaa caatggggga aaacaggcac tagagacggt tcaaaggttg     1440

ttgcccgttc tgtgccagga ccacggcttg acaccggatc aggtggtagc tatcgcttca     1500

cacgatggcg gaaaacaggc tttagaaaca gtccaaagac ttctcccagt cctttgtcag     1560

gaccacggat tgactccaga tcaagtcgtt gctattgcaa gtaatggtgg tggtaagcaa     1620

gctttagaaa ccgtacagag gcttttgcca gtgctgtgcc aggaccatgg actgacccct     1680

gatcaagtgg tagcaattgc atctcatgat ggaggaaaac aagctctgga aagcattgtg     1740

gcccagctga gccggcctga tccggcgttg gccgcgttga ccaacgacca cctcgtcgcc     1800

ttggcctgcc tcggcggacg tcctgccatg gatgcagtga aaaagggatt gccgcacgcg     1860

ccggaattga tcagaagagt caatcgccgt attggcgaac gcacgtccca tcgcgttgcg     1920

atatctagag tgggaggaag ctctcgcaga gagtccatca acccatggac tctgactggt     1980

ttcgctgatg ccgaaggatc attcgggcta agcatcctca acagaaacag aggtactgct     2040

agataccaca ctcgactgtc attcacaatc atgctgcaca acaaggacaa atcgattctg     2100

gagaatatcc agtcgacttg gaaggtcggc agcatcctca acaatggcga ccactacgtc     2160

tcgctggtgg tctaccgttt cgaagatttg aaagtgatta tcgaccactt cgagaaatat     2220

ccgctgataa cacagaaatt gggcgattac aagttgttta aacaggcatt cagcgtcatg     2280

gagaacaaag aacatcttaa ggagaatggg attaaggagc tcgtacgaat caaagctaag     2340

atgaattggg gtctcaatga cgaattgaaa aaagcatttc cagaggtgat tagcagggag     2400

cgccccctta tcaataagaa cattccgaat gggaaatggc tggctggatt cacatctggt     2460

gatggctcct tcttcgtgcg cctaagaaag tctaatgtta atgctagagt acgtgtgcaa     2520

ctggtattcg agatctcaca gcacatcaga gacaagaacc tgatgaattc attgataaca     2580

tacctaggct gtggtcacat ctacgaggga aacaaatctg agcgcagttg gctccaattc     2640

agagtagaaa aattcagcga tatcaacgac aagatcattc cggtattcca ggaaaatact     2700

ctgattggca tgaaactcga ggactttgaa gattggtgca aggttgccaa attgatcgaa     2760

gagaagaaac acctgaccga atccggtttg gatgagatta agaaaatcaa gctgaacatg     2820

aacaaaagac gtggtggagg cggtagcgga ggcggagggt cggctagcct gcggatcata     2880

gacacggaaa cctgtggact ccagggtggt atcgtggaga tagccagtgt cgatgtaatt     2940

gatgggaaaa tcgtaaaccc gatgagccac ctcgtccgac cggataggcc gataagtccg     3000

caggctatgg ctatacatag aatcacggag gctatggtag ctgacaagcc ttggatcgag     3060

gacgttatcc cacactacta tgggagcgaa tggtatgtag cgcacaacgc atcatttgat     3120

aggcgcgtgc ttcccgaaat gcctggggag tggatttgca caatgaaact cgctcgccga     3180

ctgtggcctg gtatcaagta tagtaacatg gcactttaca agacccgcaa actcaacgta     3240

cagacacctc cgggtctcca ccaccatagg gcactctatg attgttacat aaccgctgct     3300

ctccttatag acataatgaa tacctccggg tggaccgccg aacaaatggc agacataacg     3360

ggaagaccta gtttgatgac aacctttaca tttggaaagt atcggggcaa ggcggtgagc     3420

gatgtggccg aaagagatcc cggttatctt agatggctct tcaataactt ggattctatg     3480

tcacctgaac ttcgccttac cctgaagcat taccttgaga atacctga                  3528


<210>  81
<211>  3528
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - PD-1 megaTAL-ExoX  RNA construct

<400>  81
augggauccu gcaggccacc uaagaagaaa cgcaaagucg uggaucuacg cacgcucggc       60

uacagucagc agcagcaaga gaagaucaaa ccgaaggugc guucgacagu ggcgcagcac      120

cacgaggcac uggugggcca uggguuuaca cacgcgcaca ucguugcgcu cagccaacac      180

ccggcagcgu uagggaccgu cgcugucacg uaucagcaca uaaucacggc guugccagag      240

gcgacacacg aagacaucgu uggcgucggc aaacaguggu ccggcgcacg cgcccuggag      300

gccuugcuca cggaugcggg ggaguugaga gguccgccgu uacaguugga cacaggccaa      360

cuugugaaga uugcaaaacg uggcggcgug accgcaaugg aggcagugca ugcaucgcgc      420

aaugcacuga cgggugcccc ccugaaccua accccugauc agguagucgc uauagcuuca      480

aacaacgggg gcaagcaagc acuggagacc guucaacgac uccugccagu gcucugccaa      540

gaccacggac uuacgccaga ucaggugguu gcuauugccu ccaacaaugg cgggaaacaa      600

gcguuggaaa cugugcagag acuguuaccu gucuuguguc aagaccacgg ccucacgcca      660

gaucaggugg uagccauagc gucgaaugga ggugguaagc aagcccuuga aacgguccag      720

cgucuucugc cgguguugug ccaggaccac ggacuaacgc cggaucaggu cguagccauu      780

gcuucaaaua acggcggcaa acaggcgcua gagacagucc agcgccucuu gccuguguua      840

ugccaggauc acggcuuaac cccagaccaa guuguggcua uugcaucuaa caaugguggc      900

aaacaagccu uggagacagu gcaacgauua cugccugucu uaugucagga ucauggccug      960

acgcccgauc agguaguggc aaucgcaucu aauaauggag guaagcaagc acuggagacu     1020

guccagagau uguuacccgu acuaugucaa gaucaugguu ugacgccuga ucagguuguu     1080

gcgauagcca gcaacaacgg agggaaacag gcucuugaaa ccguacagcg acuucuccca     1140

gucuugugcc aagaucacgg gcuuacuccu gaucaagucg uagcuaucgc cagccacgac     1200

ggugggaaac aggcccugga aaccguacaa cgucuccucc caguacuuug ucaagaccac     1260

ggguugacuc cggaucaagu cgucgcgauc gcgagcaaug gaggggggaa gcaggcgcug     1320

gaaacuguuc agagacugcu gccuguacuu ugucaggacc auggucugac accugaccaa     1380

guuguggcga uagccaguaa caauggggga aaacaggcac uagagacggu ucaaagguug     1440

uugcccguuc ugugccagga ccacggcuug acaccggauc aggugguagc uaucgcuuca     1500

cacgauggcg gaaaacaggc uuuagaaaca guccaaagac uucucccagu ccuuugucag     1560

gaccacggau ugacuccaga ucaagucguu gcuauugcaa guaauggugg ugguaagcaa     1620

gcuuuagaaa ccguacagag gcuuuugcca gugcugugcc aggaccaugg acugaccccu     1680

gaucaagugg uagcaauugc aucucaugau ggaggaaaac aagcucugga aagcauugug     1740

gcccagcuga gccggccuga uccggcguug gccgcguuga ccaacgacca ccucgucgcc     1800

uuggccugcc ucggcggacg uccugccaug gaugcaguga aaaagggauu gccgcacgcg     1860

ccggaauuga ucagaagagu caaucgccgu auuggcgaac gcacguccca ucgcguugcg     1920

auaucuagag ugggaggaag cucucgcaga gaguccauca acccauggac ucugacuggu     1980

uucgcugaug ccgaaggauc auucgggcua agcauccuca acagaaacag agguacugcu     2040

agauaccaca cucgacuguc auucacaauc augcugcaca acaaggacaa aucgauucug     2100

gagaauaucc agucgacuug gaaggucggc agcauccuca acaauggcga ccacuacguc     2160

ucgcuggugg ucuaccguuu cgaagauuug aaagugauua ucgaccacuu cgagaaauau     2220

ccgcugauaa cacagaaauu gggcgauuac aaguuguuua aacaggcauu cagcgucaug     2280

gagaacaaag aacaucuuaa ggagaauggg auuaaggagc ucguacgaau caaagcuaag     2340

augaauuggg gucucaauga cgaauugaaa aaagcauuuc cagaggugau uagcagggag     2400

cgcccccuua ucaauaagaa cauuccgaau gggaaauggc uggcuggauu cacaucuggu     2460

gauggcuccu ucuucgugcg ccuaagaaag ucuaauguua augcuagagu acgugugcaa     2520

cugguauucg agaucucaca gcacaucaga gacaagaacc ugaugaauuc auugauaaca     2580

uaccuaggcu guggucacau cuacgaggga aacaaaucug agcgcaguug gcuccaauuc     2640

agaguagaaa aauucagcga uaucaacgac aagaucauuc cgguauucca ggaaaauacu     2700

cugauuggca ugaaacucga ggacuuugaa gauuggugca agguugccaa auugaucgaa     2760

gagaagaaac accugaccga auccgguuug gaugagauua agaaaaucaa gcugaacaug     2820

aacaaaagac gugguggagg cgguagcgga ggcggagggu cggcuagccu gcggaucaua     2880

gacacggaaa ccuguggacu ccaggguggu aucguggaga uagccagugu cgauguaauu     2940

gaugggaaaa ucguaaaccc gaugagccac cucguccgac cggauaggcc gauaaguccg     3000

caggcuaugg cuauacauag aaucacggag gcuaugguag cugacaagcc uuggaucgag     3060

gacguuaucc cacacuacua ugggagcgaa ugguauguag cgcacaacgc aucauuugau     3120

aggcgcgugc uucccgaaau gccuggggag uggauuugca caaugaaacu cgcucgccga     3180

cuguggccug guaucaagua uaguaacaug gcacuuuaca agacccgcaa acucaacgua     3240

cagacaccuc cgggucucca ccaccauagg gcacucuaug auuguuacau aaccgcugcu     3300

cuccuuauag acauaaugaa uaccuccggg uggaccgccg aacaaauggc agacauaacg     3360

ggaagaccua guuugaugac aaccuuuaca uuuggaaagu aucggggcaa ggcggugagc     3420

gauguggccg aaagagaucc cgguuaucuu agauggcucu ucaauaacuu ggauucuaug     3480

ucaccugaac uucgccuuac ccugaagcau uaccuugaga auaccuga                  3528


<210>  82
<211>  1175
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - PD-1 megaTAL-ExoX protein construct

<400>  82

Met Gly Ser Cys Arg Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu 
1               5                   10                  15      


Arg Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys 
            20                  25                  30          


Val Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly 
        35                  40                  45              


Phe Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu 
    50                  55                  60                  


Gly Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu 
65                  70                  75                  80  


Ala Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala 
                85                  90                  95      


Arg Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro 
            100                 105                 110         


Pro Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly 
        115                 120                 125             


Gly Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr 
    130                 135                 140                 


Gly Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
145                 150                 155                 160 


Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
                165                 170                 175     


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
            180                 185                 190         


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
        195                 200                 205             


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
    210                 215                 220                 


Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
225                 230                 235                 240 


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
                245                 250                 255     


Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr 
            260                 265                 270         


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
        275                 280                 285             


Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu 
    290                 295                 300                 


Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu 
305                 310                 315                 320 


Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly Lys Gln 
                325                 330                 335     


Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His 
            340                 345                 350         


Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly Gly 
        355                 360                 365             


Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln 
    370                 375                 380                 


Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser His Asp 
385                 390                 395                 400 


Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu 
                405                 410                 415     


Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser 
            420                 425                 430         


Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro 
        435                 440                 445             


Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile 
    450                 455                 460                 


Ala Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu 
465                 470                 475                 480 


Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val 
                485                 490                 495     


Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu Glu Thr Val Gln 
            500                 505                 510         


Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln 
        515                 520                 525             


Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Thr 
    530                 535                 540                 


Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro 
545                 550                 555                 560 


Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala Leu 
                565                 570                 575     


Glu Ser Ile Val Ala Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala 
            580                 585                 590         


Leu Thr Asn Asp His Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro 
        595                 600                 605             


Ala Met Asp Ala Val Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile 
    610                 615                 620                 


Arg Arg Val Asn Arg Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala 
625                 630                 635                 640 


Ile Ser Arg Val Gly Gly Ser Ser Arg Arg Glu Ser Ile Asn Pro Trp 
                645                 650                 655     


Thr Leu Thr Gly Phe Ala Asp Ala Glu Gly Ser Phe Gly Leu Ser Ile 
            660                 665                 670         


Leu Asn Arg Asn Arg Gly Thr Ala Arg Tyr His Thr Arg Leu Ser Phe 
        675                 680                 685             


Thr Ile Met Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln 
    690                 695                 700                 


Ser Thr Trp Lys Val Gly Ser Ile Leu Asn Asn Gly Asp His Tyr Val 
705                 710                 715                 720 


Ser Leu Val Val Tyr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His 
                725                 730                 735     


Phe Glu Lys Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu 
            740                 745                 750         


Phe Lys Gln Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu 
        755                 760                 765             


Asn Gly Ile Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly 
    770                 775                 780                 


Leu Asn Asp Glu Leu Lys Lys Ala Phe Pro Glu Val Ile Ser Arg Glu 
785                 790                 795                 800 


Arg Pro Leu Ile Asn Lys Asn Ile Pro Asn Gly Lys Trp Leu Ala Gly 
                805                 810                 815     


Phe Thr Ser Gly Asp Gly Ser Phe Phe Val Arg Leu Arg Lys Ser Asn 
            820                 825                 830         


Val Asn Ala Arg Val Arg Val Gln Leu Val Phe Glu Ile Ser Gln His 
        835                 840                 845             


Ile Arg Asp Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys 
    850                 855                 860                 


Gly His Ile Tyr Glu Gly Asn Lys Ser Glu Arg Ser Trp Leu Gln Phe 
865                 870                 875                 880 


Arg Val Glu Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe 
                885                 890                 895     


Gln Glu Asn Thr Leu Ile Gly Met Lys Leu Glu Asp Phe Glu Asp Trp 
            900                 905                 910         


Cys Lys Val Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser 
        915                 920                 925             


Gly Leu Asp Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Arg Arg 
    930                 935                 940                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Ser Leu Arg Ile Ile 
945                 950                 955                 960 


Asp Thr Glu Thr Cys Gly Leu Gln Gly Gly Ile Val Glu Ile Ala Ser 
                965                 970                 975     


Val Asp Val Ile Asp Gly Lys Ile Val Asn Pro Met Ser His Leu Val 
            980                 985                 990         


Arg Pro Asp Arg Pro Ile Ser Pro  Gln Ala Met Ala Ile  His Arg Ile 
        995                 1000                 1005             


Thr Glu  Ala Met Val Ala Asp  Lys Pro Trp Ile Glu  Asp Val Ile 
    1010                 1015                 1020             


Pro His  Tyr Tyr Gly Ser Glu  Trp Tyr Val Ala His  Asn Ala Ser 
    1025                 1030                 1035             


Phe Asp  Arg Arg Val Leu Pro  Glu Met Pro Gly Glu  Trp Ile Cys 
    1040                 1045                 1050             


Thr Met  Lys Leu Ala Arg Arg  Leu Trp Pro Gly Ile  Lys Tyr Ser 
    1055                 1060                 1065             


Asn Met  Ala Leu Tyr Lys Thr  Arg Lys Leu Asn Val  Gln Thr Pro 
    1070                 1075                 1080             


Pro Gly  Leu His His His Arg  Ala Leu Tyr Asp Cys  Tyr Ile Thr 
    1085                 1090                 1095             


Ala Ala  Leu Leu Ile Asp Ile  Met Asn Thr Ser Gly  Trp Thr Ala 
    1100                 1105                 1110             


Glu Gln  Met Ala Asp Ile Thr  Gly Arg Pro Ser Leu  Met Thr Thr 
    1115                 1120                 1125             


Phe Thr  Phe Gly Lys Tyr Arg  Gly Lys Ala Val Ser  Asp Val Ala 
    1130                 1135                 1140             


Glu Arg  Asp Pro Gly Tyr Leu  Arg Trp Leu Phe Asn  Asn Leu Asp 
    1145                 1150                 1155             


Ser Met  Ser Pro Glu Leu Arg  Leu Thr Leu Lys His  Tyr Leu Glu 
    1160                 1165                 1170             


Asn Thr  
    1175 


<210>  83
<211>  870
<212>  RNA
<213>  Homo sapiens

<400>  83
augguccaga ucccacaggc gcccuggcca gucgucuggg cggugcuaca acugggcugg       60

cggccaggau gguucuuaga cuccccagac aggcccugga acccccccac cuucucccca      120

gcccugcucg uggugaccga aggggacaac gccaccuuca ccugcagcuu cuccaacaca      180

ucggagagcu ucgugcuaaa cugguaccgc augagcccca gcaaccagac ggacaagcug      240

gccgccuucc ccgaggaccg cagccagccc ggccaggacu gccgcuuccg ugucacacaa      300

cugcccaacg ggcgugacuu ccacaugagc guggucaggg cccggcgcaa ugacagcggc      360

accuaccucu guggggccau cucccuggcc cccaaggcgc agaucaaaga gagccugcgg      420

gcagagcuca gggugacaga gagaagggca gaagugccca cagcccaccc cagccccuca      480

cccaggccag ccggccaguu ccaaacccug gugguuggug ucgugggcgg ccugcugggc      540

agccuggugc ugcuagucug gguccuggcc gucaucugcu cccgggccgc acgagggaca      600

auaggagcca ggcgcaccgg ccagccccug aaggaggacc ccucagccgu gccuguguuc      660

ucuguggacu auggggagcu ggauuuccag uggcgagaga agaccccgga gccccccgug      720

cccugugucc cugagcagac ggaguaugcc accauugucu uuccuagcgg aaugggcacc      780

ucaucccccg cccgcagggg cucagcugac ggcccucgga gugcccagcc acugaggccu      840

gaggauggac acugcucuug gccccucuga                                       870


<210>  84
<211>  869
<212>  RNA
<213>  Artificial sequence

<220>
<223>  mock-edited PD-1 open reading frame with deletion

<400>  84
augguccaga cccacaggcg cccuggccag ucgucugggc ggugcuacaa cugggcuggc       60

ggccaggaug guucuuagac uccccagaca ggcccuggaa cccccccacc uucuccccag      120

cccugcucgu ggugaccgaa ggggacaacg ccaccuucac cugcagcuuc uccaacacau      180

cggagagcuu cgugcuaaac ugguaccgca ugagccccag caaccagacg gacaagcugg      240

ccgccuuccc cgaggaccgc agccagcccg gccaggacug ccgcuuccgu gucacacaac      300

ugcccaacgg gcgugacuuc cacaugagcg uggucagggc ccggcgcaau gacagcggca      360

ccuaccucug uggggccauc ucccuggccc ccaaggcgca gaucaaagag agccugcggg      420

cagagcucag ggugacagag agaagggcag aagugcccac agcccacccc agccccucac      480

ccaggccagc cggccaguuc caaacccugg ugguuggugu cgugggcggc cugcugggca      540

gccuggugcu gcuagucugg guccuggccg ucaucugcuc ccgggccgca cgagggacaa      600

uaggagccag gcgcaccggc cagccccuga aggaggaccc cucagccgug ccuguguucu      660

cuguggacua uggggagcug gauuuccagu ggcgagagaa gaccccggag ccccccgugc      720

ccuguguccc ugagcagacg gaguaugcca ccauugucuu uccuagcgga augggcaccu      780

caucccccgc ccgcaggggc ucagcugacg gcccucggag ugcccagcca cugaggccug      840

aggauggaca cugcucuugg ccccucuga                                        869


<210>  85
<211>  868
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  mock-edited PD-1 open reading frame with deletion

<400>  85
augguccaga ccacaggcgc ccuggccagu cgucugggcg gugcuacaac ugggcuggcg       60

gccaggaugg uucuuagacu ccccagacag gcccuggaac ccccccaccu ucuccccagc      120

ccugcucgug gugaccgaag gggacaacgc caccuucacc ugcagcuucu ccaacacauc      180

ggagagcuuc gugcuaaacu gguaccgcau gagccccagc aaccagacgg acaagcuggc      240

cgccuucccc gaggaccgca gccagcccgg ccaggacugc cgcuuccgug ucacacaacu      300

gcccaacggg cgugacuucc acaugagcgu ggucagggcc cggcgcaaug acagcggcac      360

cuaccucugu ggggccaucu cccuggcccc caaggcgcag aucaaagaga gccugcgggc      420

agagcucagg gugacagaga gaagggcaga agugcccaca gcccacccca gccccucacc      480

caggccagcc ggccaguucc aaacccuggu gguugguguc gugggcggcc ugcugggcag      540

ccuggugcug cuagucuggg uccuggccgu caucugcucc cgggccgcac gagggacaau      600

aggagccagg cgcaccggcc agccccugaa ggaggacccc ucagccgugc cuguguucuc      660

uguggacuau ggggagcugg auuuccagug gcgagagaag accccggagc cccccgugcc      720

cugugucccu gagcagacgg aguaugccac cauugucuuu ccuagcggaa ugggcaccuc      780

aucccccgcc cgcaggggcu cagcugacgg cccucggagu gcccagccac ugaggccuga      840

ggauggacac ugcucuuggc cccucuga                                         868


<210>  86
<211>  867
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  mock-edited PD-1 open reading frame with deletion

<400>  86
augguccagc cacaggcgcc cuggccaguc gucugggcgg ugcuacaacu gggcuggcgg       60

ccaggauggu ucuuagacuc cccagacagg cccuggaacc cccccaccuu cuccccagcc      120

cugcucgugg ugaccgaagg ggacaacgcc accuucaccu gcagcuucuc caacacaucg      180

gagagcuucg ugcuaaacug guaccgcaug agccccagca accagacgga caagcuggcc      240

gccuuccccg aggaccgcag ccagcccggc caggacugcc gcuuccgugu cacacaacug      300

cccaacgggc gugacuucca caugagcgug gucagggccc ggcgcaauga cagcggcacc      360

uaccucugug gggccaucuc ccuggccccc aaggcgcaga ucaaagagag ccugcgggca      420

gagcucaggg ugacagagag aagggcagaa gugcccacag cccaccccag ccccucaccc      480

aggccagccg gccaguucca aacccuggug guuggugucg ugggcggccu gcugggcagc      540

cuggugcugc uagucugggu ccuggccguc aucugcuccc gggccgcacg agggacaaua      600

ggagccaggc gcaccggcca gccccugaag gaggaccccu cagccgugcc uguguucucu      660

guggacuaug gggagcugga uuuccagugg cgagagaaga ccccggagcc ccccgugccc      720

ugugucccug agcagacgga guaugccacc auugucuuuc cuagcggaau gggcaccuca      780

ucccccgccc gcaggggcuc agcugacggc ccucggagug cccagccacu gaggccugag      840

gauggacacu gcucuuggcc ccucuga                                          867


<210>  87
<211>  891
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of I-OnuI LHE

<400>  87
tctcgcagag agtccatcaa cccatggatt ctgactggtt tcgctgatgc cgaaggatca       60

ttcatactag acatccgcaa ccgaaacaac gaaagcaaca gataccgaac ttcgctgaga      120

ttccagatca ccctgcacaa caaggacaaa tcgattctgg agaatatcca gtccacttgg      180

aaggtcggca agatcacaaa cagcagtgac agagccgtca tgctgagggt cacccgtttc      240

gaagatttga aagtgattat cgaccacttc gagaaatatc cgctgattac ccagaaattg      300

ggcgattaca agttgtttaa acaggcattc agcgtcatgg agaataaaga acatcttaag      360

gagaatggga ttaaggagct cgtacgaatc aaagctaaga tgaattgggg tctcaatgac      420

gaattgaaaa aagcatttcc agagaacatt agcaaagagc gcccccttat caataagaac      480

attccgaatt tcaaatggct ggctggattc acagctggtg aaggctactt cggcgtgaat      540

ctaaaaaagg taaagggcac cgcaaaggta tacgtgggac tgagattctc aatctcacag      600

cacatcagag acaagaacct gatgaattca ttgataacat acctaggctg tggttccatc      660

tgggagaaga acaagtctga gttcagatgg ctcgagttcg tcgtaaccaa attcagcgat      720

atcaacgaca agatcattcc ggtattccag gaaaatactc tgattggcgt caaactcgag      780

gactttgaag attggtgcaa ggttgccaaa ttgatcaaag agaagaaaca cctgaccgaa      840

tccggtttgg atgagattaa gaaaatcaag ctgaacatga acaaaggtcg t               891


<210>  88
<211>  891
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of I-OnuI LHE

<400>  88
ucucgcagag aguccaucaa cccauggauu cugacugguu ucgcugaugc cgaaggauca       60

uucauacuag acauccgcaa ccgaaacaac gaaagcaaca gauaccgaac uucgcugaga      120

uuccagauca cccugcacaa caaggacaaa ucgauucugg agaauaucca guccacuugg      180

aaggucggca agaucacaaa cagcagugac agagccguca ugcugagggu cacccguuuc      240

gaagauuuga aagugauuau cgaccacuuc gagaaauauc cgcugauuac ccagaaauug      300

ggcgauuaca aguuguuuaa acaggcauuc agcgucaugg agaauaaaga acaucuuaag      360

gagaauggga uuaaggagcu cguacgaauc aaagcuaaga ugaauugggg ucucaaugac      420

gaauugaaaa aagcauuucc agagaacauu agcaaagagc gcccccuuau caauaagaac      480

auuccgaauu ucaaauggcu ggcuggauuc acagcuggug aaggcuacuu cggcgugaau      540

cuaaaaaagg uaaagggcac cgcaaaggua uacgugggac ugagauucuc aaucucacag      600

cacaucagag acaagaaccu gaugaauuca uugauaacau accuaggcug ugguuccauc      660

ugggagaaga acaagucuga guucagaugg cucgaguucg ucguaaccaa auucagcgau      720

aucaacgaca agaucauucc gguauuccag gaaaauacuc ugauuggcgu caaacucgag      780

gacuuugaag auuggugcaa gguugccaaa uugaucaaag agaagaaaca ccugaccgaa      840

uccgguuugg augagauuaa gaaaaucaag cugaacauga acaaaggucg u               891


<210>  89
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of I-OnuI LHE


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Xaa is any amino acid or absent

<220>
<221>  MOD_RES
<222>  (2)..(2)
<223>  Xaa is any amino acid or absent

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Xaa is any amino acid or absent

<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  Xaa is any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(302)
<223>  Xaa is any amino acid or absent

<220>
<221>  MOD_RES
<222>  (303)..(303)
<223>  Xaa is any amino acid or absent

<400>  89

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Ile Leu Asp Ile Arg Asn Arg 
            20                  25                  30          


Asn Asn Glu Ser Asn Arg Tyr Arg Thr Ser Leu Arg Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Thr Asn Ser Ser Asp Arg Ala Val Met Leu Arg 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ala 
                165                 170                 175     


Gly Glu Gly Tyr Phe Gly Val Asn Leu Lys Lys Val Lys Gly Thr Ala 
            180                 185                 190         


Lys Val Tyr Val Gly Leu Arg Phe Ser Ile Ser Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Ser Ile 
    210                 215                 220                 


Trp Glu Lys Asn Lys Ser Glu Phe Arg Trp Leu Glu Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Lys Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  90
<211>  882
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of I-OnuI LHE

<400>  90
tccatcaacc catggattct gactggtttc gctgatgccg aaggatcatt catactagac       60

atccgcaacc gaaacaacga aagcaacaga taccgaactt cgctgagatt ccagatcacc      120

ctgcacaaca aggacaaatc gattctggag aatatccagt ccacttggaa ggtcggcaag      180

atcacaaaca gcagtgacag agccgtcatg ctgagggtca cccgtttcga agatttgaaa      240

gtgattatcg accacttcga gaaatatccg ctgattaccc agaaattggg cgattacaag      300

ttgtttaaac aggcattcag cgtcatggag aataaagaac atcttaagga gaatgggatt      360

aaggagctcg tacgaatcaa agctaagatg aattggggtc tcaatgacga attgaaaaaa      420

gcatttccag agaacattag caaagagcgc ccccttatca ataagaacat tccgaatttc      480

aaatggctgg ctggattcac agctggtgat ggccatttcg gcgtgaatct aaaaaaggta      540

aagggcaccg caaaggtata cgtgggactg agattcgcta tctcacagca catcagagac      600

aagaacctga tgaattcatt gataacatac ctaggctgtg gttccatcag agagaagaac      660

aagtctgagt tcagatggct cgagttcgaa gtaaccaaat tcagcgatat caacgacaag      720

atcattccgg tattccagga aaatactctg attggcgtca aactcgagga ctttgaagat      780

tggtgcaagg ttgccaaatt gatcgaagag aagaaacacc tgaccgaatc cggtttggat      840

gagattaaga aaatcaagct gaacatgaac aaaggtcgtt ga                         882


<210>  91
<211>  882
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of I-OnuI LHE

<400>  91
uccaucaacc cauggauucu gacugguuuc gcugaugccg aaggaucauu cauacuagac       60

auccgcaacc gaaacaacga aagcaacaga uaccgaacuu cgcugagauu ccagaucacc      120

cugcacaaca aggacaaauc gauucuggag aauauccagu ccacuuggaa ggucggcaag      180

aucacaaaca gcagugacag agccgucaug cugaggguca cccguuucga agauuugaaa      240

gugauuaucg accacuucga gaaauauccg cugauuaccc agaaauuggg cgauuacaag      300

uuguuuaaac aggcauucag cgucauggag aauaaagaac aucuuaagga gaaugggauu      360

aaggagcucg uacgaaucaa agcuaagaug aauugggguc ucaaugacga auugaaaaaa      420

gcauuuccag agaacauuag caaagagcgc ccccuuauca auaagaacau uccgaauuuc      480

aaauggcugg cuggauucac agcuggugau ggccauuucg gcgugaaucu aaaaaaggua      540

aagggcaccg caaagguaua cgugggacug agauucgcua ucucacagca caucagagac      600

aagaaccuga ugaauucauu gauaacauac cuaggcugug guuccaucag agagaagaac      660

aagucugagu ucagauggcu cgaguucgaa guaaccaaau ucagcgauau caacgacaag      720

aucauuccgg uauuccagga aaauacucug auuggcguca aacucgagga cuuugaagau      780

uggugcaagg uugccaaauu gaucgaagag aagaaacacc ugaccgaauc cgguuuggau      840

gagauuaaga aaaucaagcu gaacaugaac aaaggucguu ga                         882


<210>  92
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of I-OnuI LHE


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Xaa is any amino acid or absent

<220>
<221>  MOD_RES
<222>  (2)..(2)
<223>  Xaa is any amino acid or absent

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Xaa is any amino acid or absent

<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  Xaa is any amino acid or absent

<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  Xaa is any amino acid or absent

<220>
<221>  MOD_RES
<222>  (6)..(6)
<223>  Xaa is any amino acid or absent

<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  Xaa is any amino acid or absent

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  Xaa is any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(302)
<223>  Xaa is any amino acid or absent

<220>
<221>  MOD_RES
<222>  (303)..(303)
<223>  Xaa is any amino acid or absent

<400>  92

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Ile Leu Asp Ile Arg Asn Arg 
            20                  25                  30          


Asn Asn Glu Ser Asn Arg Tyr Arg Thr Ser Leu Arg Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Thr Asn Ser Ser Asp Arg Ala Val Met Leu Arg 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ala 
                165                 170                 175     


Gly Asp Gly His Phe Gly Val Asn Leu Lys Lys Val Lys Gly Thr Ala 
            180                 185                 190         


Lys Val Tyr Val Gly Leu Arg Phe Ala Ile Ser Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Ser Ile 
    210                 215                 220                 


Arg Glu Lys Asn Lys Ser Glu Phe Arg Trp Leu Glu Phe Glu Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  93
<211>  891
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of I-OnuI LHE

<400>  93
tcgcgcagag agtccatcaa cccatggatt ctgactggtt tcgctgatgc cgaaggatgc       60

ttccgactag acatccgcaa cgcaaactcc atgcgtgtaa aatacagaac tagactgtcc      120

ttcgaaatcg tactgcacaa caaggacaaa tcgattctgg agaatatcca gtcgacttgg      180

aaggtcggca aaatcacaaa ctacggcgac agaggagtcc gcctgagagt cggccgtttc      240

gaagatttga aagtgattat cgaccacttc gagaaatatc cgctgataac acagaaattg      300

ggcgattaca agttgtttaa acaggcattc agcgtcatgg agaacaaaga acatcttaag      360

gagaatggga ttaaggagct cgtacgaatc aaagctaaga tgaattgggg tctcaatgac      420

gaattgaaaa aagcatttcc agagaacatt agcaaagagc gcccccttat caataagaac      480

atccccaatt tcaaatggct ggctggattc acatctggtg acggctcgtt catggtggaa      540

ctaatgaaga ataagaataa cgttattgta cgtgtgcgtc tgagattctc aatctcccag      600

cacatcagag acaagaacct gatgaattca ttgataacat acctaggctg tggtcgtatc      660

gttgagaata acaaatctga gcacagttgg ctcgaattca ttgtaacaaa attcagcgat      720

atcaacgaca agatcattcc ggtattccag gaaaatactc tgattggcgt caaactcgag      780

gactttgaag attggtgcaa ggttgccaaa ttgatcgaag agaagaaaca cctgaccgaa      840

tccggtttgg atgagattaa gaaaatcaag ctgaacatga acaaaggtcg t               891


<210>  94
<211>  891
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of I-OnuI LHE

<400>  94
ucgcgcagag aguccaucaa cccauggauu cugacugguu ucgcugaugc cgaaggaugc       60

uuccgacuag acauccgcaa cgcaaacucc augcguguaa aauacagaac uagacugucc      120

uucgaaaucg uacugcacaa caaggacaaa ucgauucugg agaauaucca gucgacuugg      180

aaggucggca aaaucacaaa cuacggcgac agaggagucc gccugagagu cggccguuuc      240

gaagauuuga aagugauuau cgaccacuuc gagaaauauc cgcugauaac acagaaauug      300

ggcgauuaca aguuguuuaa acaggcauuc agcgucaugg agaacaaaga acaucuuaag      360

gagaauggga uuaaggagcu cguacgaauc aaagcuaaga ugaauugggg ucucaaugac      420

gaauugaaaa aagcauuucc agagaacauu agcaaagagc gcccccuuau caauaagaac      480

auccccaauu ucaaauggcu ggcuggauuc acaucuggug acggcucguu caugguggaa      540

cuaaugaaga auaagaauaa cguuauugua cgugugcguc ugagauucuc aaucucccag      600

cacaucagag acaagaaccu gaugaauuca uugauaacau accuaggcug uggucguauc      660

guugagaaua acaaaucuga gcacaguugg cucgaauuca uuguaacaaa auucagcgau      720

aucaacgaca agaucauucc gguauuccag gaaaauacuc ugauuggcgu caaacucgag      780

gacuuugaag auuggugcaa gguugccaaa uugaucgaag agaagaaaca ccugaccgaa      840

uccgguuugg augagauuaa gaaaaucaag cugaacauga acaaaggucg u               891


<210>  95
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of I-OnuI LHE


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Xaa is any amino acid or absent

<220>
<221>  MOD_RES
<222>  (2)..(2)
<223>  Xaa is any amino acid or absent

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Xaa is any amino acid or absent

<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  Xaa is any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(302)
<223>  Xaa is any amino acid or absent

<220>
<221>  MOD_RES
<222>  (303)..(303)
<223>  Xaa is any amino acid or absent

<400>  95

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Cys Phe Arg Leu Asp Ile Arg Asn Ala 
            20                  25                  30          


Asn Ser Met Arg Val Lys Tyr Arg Thr Arg Leu Ser Phe Glu Ile Val 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Thr Asn Tyr Gly Asp Arg Gly Val Arg Leu Arg 
65                  70                  75                  80  


Val Gly Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Ser Phe Met Val Glu Leu Met Lys Asn Lys Asn Asn Val 
            180                 185                 190         


Ile Val Arg Val Arg Leu Arg Phe Ser Ile Ser Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Arg Ile 
    210                 215                 220                 


Val Glu Asn Asn Lys Ser Glu His Ser Trp Leu Glu Phe Ile Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  96
<211>  891
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of I-OnuI LHE

<400>  96
tcgcgcagag agtccatcaa cccatggatt ctgactggtt tcgctgatgc cgaaggatgc       60

ttccgactag acatccgcaa cgcaaacgat ttaagagccg gatacagaac tagactggcc      120

ttcgaaatcg tactgcacaa caaggacaaa tcgattctgg agaatatcca gtcgacttgg      180

aaggtcggca caatctacaa cgcgggcgac aacgcagtca gactgcaagt cacacgtttc      240

gaagatttga aagtgattat cgaccacttc gagaaatatc cgctgataac acagaaattg      300

ggcgattaca agttgtttaa acaggcattc agcgtcatgg agaacaaaga acatcttaag      360

gagaatggga ttaaggagct cgtacgaatc aaagctaaga tgaattgggg tctcaatgac      420

gaattgaaaa aagcatttcc agagaacatt agcaaagagc gctcccttat caataagaac      480

attccgaatc tcaaatggct ggctggattc acatctggtg acggctcgtt cgtggtggaa      540

ctaaagaaga gaagaagccc cgtcaaggta ggagtgcggc tgcgattcag catcacccag      600

cacatcagag acaagaacct gatgaattca ttgataacat acctaggctg tggtcgtatc      660

gttgagaata acaaatctga gcacagttgg ctcgaattca ttgtaacaaa attcagcgat      720

atcaacgaca agatcattcc ggtattccag gaaaatactc tgattggcgt caaactcgag      780

gactttgaag attggtgcaa ggttgccaaa ttgatcgaag agaagaaaca cctgaccgaa      840

tccggtttgg atgagattaa gaaaatcaag ctgaacatga acaaaggtcg t               891


<210>  97
<211>  891
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of I-OnuI LHE

<400>  97
ucgcgcagag aguccaucaa cccauggauu cugacugguu ucgcugaugc cgaaggaugc       60

uuccgacuag acauccgcaa cgcaaacgau uuaagagccg gauacagaac uagacuggcc      120

uucgaaaucg uacugcacaa caaggacaaa ucgauucugg agaauaucca gucgacuugg      180

aaggucggca caaucuacaa cgcgggcgac aacgcaguca gacugcaagu cacacguuuc      240

gaagauuuga aagugauuau cgaccacuuc gagaaauauc cgcugauaac acagaaauug      300

ggcgauuaca aguuguuuaa acaggcauuc agcgucaugg agaacaaaga acaucuuaag      360

gagaauggga uuaaggagcu cguacgaauc aaagcuaaga ugaauugggg ucucaaugac      420

gaauugaaaa aagcauuucc agagaacauu agcaaagagc gcucccuuau caauaagaac      480

auuccgaauc ucaaauggcu ggcuggauuc acaucuggug acggcucguu cgugguggaa      540

cuaaagaaga gaagaagccc cgucaaggua ggagugcggc ugcgauucag caucacccag      600

cacaucagag acaagaaccu gaugaauuca uugauaacau accuaggcug uggucguauc      660

guugagaaua acaaaucuga gcacaguugg cucgaauuca uuguaacaaa auucagcgau      720

aucaacgaca agaucauucc gguauuccag gaaaauacuc ugauuggcgu caaacucgag      780

gacuuugaag auuggugcaa gguugccaaa uugaucgaag agaagaaaca ccugaccgaa      840

uccgguuugg augagauuaa gaaaaucaag cugaacauga acaaaggucg u               891


<210>  98
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of I-OnuI LHE


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Xaa is any amino acid or absent

<220>
<221>  MOD_RES
<222>  (2)..(2)
<223>  Xaa is any amino acid or absent

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Xaa is any amino acid or absent

<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  Xaa is any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(302)
<223>  Xaa is any amino acid or absent

<220>
<221>  MOD_RES
<222>  (303)..(303)
<223>  Xaa is any amino acid or absent

<400>  98

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Cys Phe Arg Leu Asp Ile Arg Asn Ala 
            20                  25                  30          


Asn Asp Leu Arg Ala Gly Tyr Arg Thr Arg Leu Ala Phe Glu Ile Val 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Thr Ile Tyr Asn Ala Gly Asp Asn Ala Val Arg Leu Gln 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Ser Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Ser Phe Val Val Glu Leu Lys Lys Arg Arg Ser Pro Val 
            180                 185                 190         


Lys Val Gly Val Arg Leu Arg Phe Ser Ile Thr Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Arg Ile 
    210                 215                 220                 


Val Glu Asn Asn Lys Ser Glu His Ser Trp Leu Glu Phe Ile Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  99
<211>  443
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of I-OnuI LHE

<400>  99
ttagcaggga gcgccccctt atcaataaga acattccgaa tgggaaatgg ctggctggat       60

tcacatctgg tgatggctcc ttcttcgtgc gcctaagaaa gtctaatgtt aatgctagag      120

tacgtgtgca actggtattc gagatctcac agcacatcag agacaagaac ctgatgaatt      180

cattgataac atacctaggc tgtggtcaca tctacgaggg aaacaaatct gagcgcagtt      240

ggctccaatt cagagtagaa aaattcagcg atatcaacga caagatcatt ccggtattcc      300

aggaaaatac tctgattggc atgaaactcg aggactttga agattggtgc aaggttgcca      360

aattgatcga agagaagaaa cacctgaccg aatccggttt ggatgagatt aagaaaatca      420

agctgaacat gaacaaaaga cgt                                              443


<210>  100
<211>  891
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Engineered variant of I-OnuI LHE

<400>  100
ucucgcagag aguccaucaa cccauggacu cugacugguu ucgcugaugc cgaaggauca       60

uucgggcuaa gcauccucaa cagaaacaga gguacugcua gauaccacac ucgacuguca      120

uucacaauca ugcugcacaa caaggacaaa ucgauucugg agaauaucca gucgacuugg      180

aaggucggca gcauccucaa caauggcgac cacuacgucu cgcugguggu cuaccguuuc      240

gaagauuuga aagugauuau cgaccacuuc gagaaauauc cgcugauaac acagaaauug      300

ggcgauuaca aguuguuuaa acaggcauuc agcgucaugg agaacaaaga acaucuuaag      360

gagaauggga uuaaggagcu cguacgaauc aaagcuaaga ugaauugggg ucucaaugac      420

gaauugaaaa aagcauuucc agaggugauu agcagggagc gcccccuuau caauaagaac      480

auuccgaaug ggaaauggcu ggcuggauuc acaucuggug auggcuccuu cuucgugcgc      540

cuaagaaagu cuaauguuaa ugcuagagua cgugugcaac ugguauucga gaucucacag      600

cacaucagag acaagaaccu gaugaauuca uugauaacau accuaggcug uggucacauc      660

uacgagggaa acaaaucuga gcgcaguugg cuccaauuca gaguagaaaa auucagcgau      720

aucaacgaca agaucauucc gguauuccag gaaaauacuc ugauuggcau gaaacucgag      780

gacuuugaag auuggugcaa gguugccaaa uugaucgaag agaagaaaca ccugaccgaa      840

uccgguuugg augagauuaa gaaaaucaag cugaacauga acaaaagacg u               891


<210>  101
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Engineered variant of I-OnuI LHE


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Xaa is any amino acid or absent

<220>
<221>  MOD_RES
<222>  (2)..(2)
<223>  Xaa is any amino acid or absent

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Xaa is any amino acid or absent

<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  Xaa is any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(302)
<223>  Xaa is any amino acid or absent

<220>
<221>  MOD_RES
<222>  (303)..(303)
<223>  Xaa is any amino acid or absent

<400>  101

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Thr Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Gly Leu Ser Ile Leu Asn Arg 
            20                  25                  30          


Asn Arg Gly Thr Ala Arg Tyr His Thr Arg Leu Ser Phe Thr Ile Met 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Ser Ile Leu Asn Asn Gly Asp His Tyr Val Ser Leu Val 
65                  70                  75                  80  


Val Tyr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Val Ile Ser Arg Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Gly Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Ser Phe Phe Val Arg Leu Arg Lys Ser Asn Val Asn Ala 
            180                 185                 190         


Arg Val Arg Val Gln Leu Val Phe Glu Ile Ser Gln His Ile Arg Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly His Ile 
    210                 215                 220                 


Tyr Glu Gly Asn Lys Ser Glu Arg Ser Trp Leu Gln Phe Arg Val Glu 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Met Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Arg Arg Xaa Xaa 
    290                 295                 300             


<210>  102
<211>  303
<212>  PRT
<213>  Ophiostoma novo-ulmi

<400>  102

Met Ala Tyr Met Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Leu Leu Arg Ile Arg Asn Asn 
            20                  25                  30          


Asn Lys Ser Ser Val Gly Tyr Ser Thr Glu Leu Gly Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Val Ile Ala Asn Ser Gly Asp Asn Ala Val Ser Leu Lys 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Met Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Cys Val Met Glu Asn Lys Glu His Leu Lys Ile Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Leu Asn Trp Gly Leu Thr Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Ile Ile Ser Lys Glu Arg Ser Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Cys Phe Phe Val Asn Leu Ile Lys Ser Lys Ser Lys Leu 
            180                 185                 190         


Gly Val Gln Val Gln Leu Val Phe Ser Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Lys Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Asp Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe 
    290                 295                 300             


<210>  103
<211>  303
<212>  PRT
<213>  Ophiostoma novo-ulmi

<400>  103

Met Ala Tyr Met Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Leu Leu Arg Ile Arg Asn Asn 
            20                  25                  30          


Asn Lys Ser Ser Val Gly Tyr Ser Thr Glu Leu Gly Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Val Ile Ala Asn Ser Gly Asp Asn Ala Val Ser Leu Lys 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Leu Asn Trp Gly Leu Thr Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Ser Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Cys Phe Phe Val Asn Leu Ile Lys Ser Lys Ser Lys Leu 
            180                 185                 190         


Gly Val Gln Val Gln Leu Val Phe Ser Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Lys Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Asp Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe 
    290                 295                 300             


<210>  104
<211>  303
<212>  PRT
<213>  Ophiostoma novo-ulmi


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (2)..(2)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Any amino acid or absent

<400>  104

Xaa Xaa Xaa Met Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Leu Leu Arg Ile Arg Asn Asn 
            20                  25                  30          


Asn Lys Ser Ser Val Gly Tyr Ser Thr Glu Leu Gly Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Val Ile Ala Asn Ser Gly Asp Asn Ala Val Ser Leu Lys 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Leu Asn Trp Gly Leu Thr Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Ser Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Cys Phe Phe Val Asn Leu Ile Lys Ser Lys Ser Lys Leu 
            180                 185                 190         


Gly Val Gln Val Gln Leu Val Phe Ser Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Lys Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Asp Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe 
    290                 295                 300             


<210>  105
<211>  303
<212>  PRT
<213>  Ophiostoma novo-ulmi


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (2)..(2)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(302)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (303)..(303)
<223>  Any amino acid or absent

<400>  105

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Leu Leu Arg Ile Arg Asn Asn 
            20                  25                  30          


Asn Lys Ser Ser Val Gly Tyr Ser Thr Glu Leu Gly Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Val Ile Ala Asn Ser Gly Asp Asn Ala Val Ser Leu Lys 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Leu Asn Trp Gly Leu Thr Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Ser Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Cys Phe Phe Val Asn Leu Ile Lys Ser Lys Ser Lys Leu 
            180                 185                 190         


Gly Val Gln Val Gln Leu Val Phe Ser Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Lys Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Asp Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  106
<211>  303
<212>  PRT
<213>  Ophiostoma novo-ulmi


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (2)..(2)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (6)..(6)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(302)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (303)..(303)
<223>  Any amino acid or absent

<400>  106

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Leu Leu Arg Ile Arg Asn Asn 
            20                  25                  30          


Asn Lys Ser Ser Val Gly Tyr Ser Thr Glu Leu Gly Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Val Ile Ala Asn Ser Gly Asp Asn Ala Val Ser Leu Lys 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Leu Asn Trp Gly Leu Thr Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Ser Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Cys Phe Phe Val Asn Leu Ile Lys Ser Lys Ser Lys Leu 
            180                 185                 190         


Gly Val Gln Val Gln Leu Val Phe Ser Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Lys Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Asp Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  107
<211>  660
<212>  DNA
<213>  Escherichia coli

<400>  107
atgctgcgga tcatagacac ggaaacctgt ggactccagg gtggtatcgt ggagatagcc       60

agtgtcgatg taattgatgg gaaaatcgta aacccgatga gccacctcgt ccgaccggat      120

aggccgataa gtccgcaggc tatggctata catagaatca cggaggctat ggtagctgac      180

aagccttgga tcgaggacgt tatcccacac tactatggga gcgaatggta tgtagcgcac      240

aacgcatcat ttgataggcg cgtgcttccc gaaatgcctg gggagtggat ttgcacaatg      300

aaactcgctc gccgactgtg gcctggtatc aagtatagta acatggcact ttacaagacc      360

cgcaaactca acgtacagac acctccgggt ctccaccacc atagggcact ctatgattgt      420

tacataaccg ctgctctcct tatagacata atgaatacct ccgggtggac cgccgaacaa      480

atggcagaca taacgggaag acctagtttg atgacaacct ttacatttgg aaagtatcgg      540

ggcaaggcgg tgagcgatgt ggccgaaaga gatcccggtt atcttagatg gctcttcaat      600

aacttggatt ctatgtcacc tgaacttcgc cttaccctga agcattacct tgagaatacc      660


<210>  108
<211>  660
<212>  RNA
<213>  Escherichia coli

<400>  108
augcugcgga ucauagacac ggaaaccugu ggacuccagg gugguaucgu ggagauagcc       60

agugucgaug uaauugaugg gaaaaucgua aacccgauga gccaccucgu ccgaccggau      120

aggccgauaa guccgcaggc uauggcuaua cauagaauca cggaggcuau gguagcugac      180

aagccuugga ucgaggacgu uaucccacac uacuauggga gcgaauggua uguagcgcac      240

aacgcaucau uugauaggcg cgugcuuccc gaaaugccug gggaguggau uugcacaaug      300

aaacucgcuc gccgacugug gccugguauc aaguauagua acauggcacu uuacaagacc      360

cgcaaacuca acguacagac accuccgggu cuccaccacc auagggcacu cuaugauugu      420

uacauaaccg cugcucuccu uauagacaua augaauaccu ccggguggac cgccgaacaa      480

auggcagaca uaacgggaag accuaguuug augacaaccu uuacauuugg aaaguaucgg      540

ggcaaggcgg ugagcgaugu ggccgaaaga gaucccgguu aucuuagaug gcucuucaau      600

aacuuggauu cuaugucacc ugaacuucgc cuuacccuga agcauuaccu ugagaauacc      660


<210>  109
<211>  220
<212>  PRT
<213>  Escherichia coli

<400>  109

Met Leu Arg Ile Ile Asp Thr Glu Thr Cys Gly Leu Gln Gly Gly Ile 
1               5                   10                  15      


Val Glu Ile Ala Ser Val Asp Val Ile Asp Gly Lys Ile Val Asn Pro 
            20                  25                  30          


Met Ser His Leu Val Arg Pro Asp Arg Pro Ile Ser Pro Gln Ala Met 
        35                  40                  45              


Ala Ile His Arg Ile Thr Glu Ala Met Val Ala Asp Lys Pro Trp Ile 
    50                  55                  60                  


Glu Asp Val Ile Pro His Tyr Tyr Gly Ser Glu Trp Tyr Val Ala His 
65                  70                  75                  80  


Asn Ala Ser Phe Asp Arg Arg Val Leu Pro Glu Met Pro Gly Glu Trp 
                85                  90                  95      


Ile Cys Thr Met Lys Leu Ala Arg Arg Leu Trp Pro Gly Ile Lys Tyr 
            100                 105                 110         


Ser Asn Met Ala Leu Tyr Lys Thr Arg Lys Leu Asn Val Gln Thr Pro 
        115                 120                 125             


Pro Gly Leu His His His Arg Ala Leu Tyr Asp Cys Tyr Ile Thr Ala 
    130                 135                 140                 


Ala Leu Leu Ile Asp Ile Met Asn Thr Ser Gly Trp Thr Ala Glu Gln 
145                 150                 155                 160 


Met Ala Asp Ile Thr Gly Arg Pro Ser Leu Met Thr Thr Phe Thr Phe 
                165                 170                 175     


Gly Lys Tyr Arg Gly Lys Ala Val Ser Asp Val Ala Glu Arg Asp Pro 
            180                 185                 190         


Gly Tyr Leu Arg Trp Leu Phe Asn Asn Leu Asp Ser Met Ser Pro Glu 
        195                 200                 205             


Leu Arg Leu Thr Leu Lys His Tyr Leu Glu Asn Thr 
    210                 215                 220 


<210>  110
<211>  1425
<212>  DNA
<213>  Escherichia coli

<400>  110
atgaacgatg gcaagcagca aagtacattc cttttccacg actacgaaac ctttggaacc       60

caccccgctc tggatcggcc agcacagttc gctgccataa gaaccgacaa cgagttcaat      120

gtgatcggtg aacccgaggt gttttactgc aaacctgcgg acgattatct gcctcaaccg      180

ggagccgttc tgattacggg aattaccccc caagaagcgc gtgccaaggg cgaaaatgaa      240

gctgcatttg ccgctagaat ccatagcctg tttactgttc cgaagacgtg tatcttggga      300

tacaacaatg tacgctttga tgacgaagta actcgtaacg tgttctatcg gaacttctat      360

gacccttacg cctggagttg gcagcacgac aattctcgat gggatcttct ggatgtgatg      420

agagcctgct acgctctcag gccagagggc attaactggc ctgagaacga cgatgggctt      480

ccgtcatttc gccttgagca tcttaccaaa gcgaatggca tcgaacactc caatgcacac      540

gacgctatgg ctgatgtcta cgcgactatt gcaatggcga aattggtcaa gacaagacag      600

ccgaggttgt ttgactacct gtttacgcat cgaaacaaac ataagctcat ggcactgata      660

gatgtccctc aaatgaagcc cctcgttcac gtcagcggta tgttcggagc gtggagagga      720

aacacgtcct gggttgctcc tctggcatgg catccagaga acagaaacgc ggtaatcatg      780

gtcgatctcg caggagacat tagcccactt ctcgaattgg attccgacac cttgcgagaa      840

cgcctttaca cagcgaaaac tgatttgggt gataacgctg ccgtacctgt caaattggta      900

catatcaaca aatgtccggt gctcgcgcaa gccaacaccc tgagacctga agacgcggat      960

agactgggga taaaccggca gcactgtttg gataatctca agatactcag ggagaatccg     1020

caggtgcgcg aaaaagtcgt agccatcttt gcggaggcag aaccatttac gccgtctgac     1080

aatgtagatg ctcagttata caatggcttc tttagtgacg ctgaccgagc tgcgatgaag     1140

attgtgctag agactgaacc gagaaacctc tctgcattgg acatcacttt tgtcgataag     1200

cgaatagaga agttgctgtt caactatcgt gcccgaaact ttccagggac actcgattac     1260

gcagagcaac aaaggtggct tgagcatcgc aggcaggtgt ttaccccaga gttcttgcag     1320

ggttacgctg aggagattca aatgttggca cagcagtatg cagatgataa ggagaaagtc     1380

gcgttgctga aggctctgtg gcaatacgcc gaagagatag tgtga                     1425


<210>  111
<211>  1425
<212>  RNA
<213>  Escherichia coli

<400>  111
augaacgaug gcaagcagca aaguacauuc cuuuuccacg acuacgaaac cuuuggaacc       60

caccccgcuc uggaucggcc agcacaguuc gcugccauaa gaaccgacaa cgaguucaau      120

gugaucggug aacccgaggu guuuuacugc aaaccugcgg acgauuaucu gccucaaccg      180

ggagccguuc ugauuacggg aauuaccccc caagaagcgc gugccaaggg cgaaaaugaa      240

gcugcauuug ccgcuagaau ccauagccug uuuacuguuc cgaagacgug uaucuuggga      300

uacaacaaug uacgcuuuga ugacgaagua acucguaacg uguucuaucg gaacuucuau      360

gacccuuacg ccuggaguug gcagcacgac aauucucgau gggaucuucu ggaugugaug      420

agagccugcu acgcucucag gccagagggc auuaacuggc cugagaacga cgaugggcuu      480

ccgucauuuc gccuugagca ucuuaccaaa gcgaauggca ucgaacacuc caaugcacac      540

gacgcuaugg cugaugucua cgcgacuauu gcaauggcga aauuggucaa gacaagacag      600

ccgagguugu uugacuaccu guuuacgcau cgaaacaaac auaagcucau ggcacugaua      660

gaugucccuc aaaugaagcc ccucguucac gucagcggua uguucggagc guggagagga      720

aacacguccu ggguugcucc ucuggcaugg cauccagaga acagaaacgc gguaaucaug      780

gucgaucucg caggagacau uagcccacuu cucgaauugg auuccgacac cuugcgagaa      840

cgccuuuaca cagcgaaaac ugauuugggu gauaacgcug ccguaccugu caaauuggua      900

cauaucaaca aauguccggu gcucgcgcaa gccaacaccc ugagaccuga agacgcggau      960

agacugggga uaaaccggca gcacuguuug gauaaucuca agauacucag ggagaauccg     1020

caggugcgcg aaaaagucgu agccaucuuu gcggaggcag aaccauuuac gccgucugac     1080

aauguagaug cucaguuaua caauggcuuc uuuagugacg cugaccgagc ugcgaugaag     1140

auugugcuag agacugaacc gagaaaccuc ucugcauugg acaucacuuu ugucgauaag     1200

cgaauagaga aguugcuguu caacuaucgu gcccgaaacu uuccagggac acucgauuac     1260

gcagagcaac aaagguggcu ugagcaucgc aggcaggugu uuaccccaga guucuugcag     1320

gguuacgcug aggagauuca aauguuggca cagcaguaug cagaugauaa ggagaaaguc     1380

gcguugcuga aggcucugug gcaauacgcc gaagagauag uguga                     1425


<210>  112
<211>  474
<212>  PRT
<213>  Escherichia coli

<400>  112

Met Asn Asp Gly Lys Gln Gln Ser Thr Phe Leu Phe His Asp Tyr Glu 
1               5                   10                  15      


Thr Phe Gly Thr His Pro Ala Leu Asp Arg Pro Ala Gln Phe Ala Ala 
            20                  25                  30          


Ile Arg Thr Asp Asn Glu Phe Asn Val Ile Gly Glu Pro Glu Val Phe 
        35                  40                  45              


Tyr Cys Lys Pro Ala Asp Asp Tyr Leu Pro Gln Pro Gly Ala Val Leu 
    50                  55                  60                  


Ile Thr Gly Ile Thr Pro Gln Glu Ala Arg Ala Lys Gly Glu Asn Glu 
65                  70                  75                  80  


Ala Ala Phe Ala Ala Arg Ile His Ser Leu Phe Thr Val Pro Lys Thr 
                85                  90                  95      


Cys Ile Leu Gly Tyr Asn Asn Val Arg Phe Asp Asp Glu Val Thr Arg 
            100                 105                 110         


Asn Val Phe Tyr Arg Asn Phe Tyr Asp Pro Tyr Ala Trp Ser Trp Gln 
        115                 120                 125             


His Asp Asn Ser Arg Trp Asp Leu Leu Asp Val Met Arg Ala Cys Tyr 
    130                 135                 140                 


Ala Leu Arg Pro Glu Gly Ile Asn Trp Pro Glu Asn Asp Asp Gly Leu 
145                 150                 155                 160 


Pro Ser Phe Arg Leu Glu His Leu Thr Lys Ala Asn Gly Ile Glu His 
                165                 170                 175     


Ser Asn Ala His Asp Ala Met Ala Asp Val Tyr Ala Thr Ile Ala Met 
            180                 185                 190         


Ala Lys Leu Val Lys Thr Arg Gln Pro Arg Leu Phe Asp Tyr Leu Phe 
        195                 200                 205             


Thr His Arg Asn Lys His Lys Leu Met Ala Leu Ile Asp Val Pro Gln 
    210                 215                 220                 


Met Lys Pro Leu Val His Val Ser Gly Met Phe Gly Ala Trp Arg Gly 
225                 230                 235                 240 


Asn Thr Ser Trp Val Ala Pro Leu Ala Trp His Pro Glu Asn Arg Asn 
                245                 250                 255     


Ala Val Ile Met Val Asp Leu Ala Gly Asp Ile Ser Pro Leu Leu Glu 
            260                 265                 270         


Leu Asp Ser Asp Thr Leu Arg Glu Arg Leu Tyr Thr Ala Lys Thr Asp 
        275                 280                 285             


Leu Gly Asp Asn Ala Ala Val Pro Val Lys Leu Val His Ile Asn Lys 
    290                 295                 300                 


Cys Pro Val Leu Ala Gln Ala Asn Thr Leu Arg Pro Glu Asp Ala Asp 
305                 310                 315                 320 


Arg Leu Gly Ile Asn Arg Gln His Cys Leu Asp Asn Leu Lys Ile Leu 
                325                 330                 335     


Arg Glu Asn Pro Gln Val Arg Glu Lys Val Val Ala Ile Phe Ala Glu 
            340                 345                 350         


Ala Glu Pro Phe Thr Pro Ser Asp Asn Val Asp Ala Gln Leu Tyr Asn 
        355                 360                 365             


Gly Phe Phe Ser Asp Ala Asp Arg Ala Ala Met Lys Ile Val Leu Glu 
    370                 375                 380                 


Thr Glu Pro Arg Asn Leu Ser Ala Leu Asp Ile Thr Phe Val Asp Lys 
385                 390                 395                 400 


Arg Ile Glu Lys Leu Leu Phe Asn Tyr Arg Ala Arg Asn Phe Pro Gly 
                405                 410                 415     


Thr Leu Asp Tyr Ala Glu Gln Gln Arg Trp Leu Glu His Arg Arg Gln 
            420                 425                 430         


Val Phe Thr Pro Glu Phe Leu Gln Gly Tyr Ala Glu Glu Ile Gln Met 
        435                 440                 445             


Leu Ala Gln Gln Tyr Ala Asp Asp Lys Glu Lys Val Ala Leu Leu Lys 
    450                 455                 460                 


Ala Leu Trp Gln Tyr Ala Glu Glu Ile Val 
465                 470                 


<210>  113
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  113

Gly Gly Gly 
1           


<210>  114
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  114

Asp Gly Gly Gly Ser 
1               5   


<210>  115
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  115

Thr Gly Glu Lys Pro 
1               5   


<210>  116
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  116

Gly Gly Arg Arg 
1               


<210>  117
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  117

Gly Gly Gly Gly Ser 
1               5   


<210>  118
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  118

Glu Gly Lys Ser Ser Gly Ser Gly Ser Glu Ser Lys Val Asp 
1               5                   10                  


<210>  119
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  119

Lys Glu Ser Gly Ser Val Ser Ser Glu Gln Leu Ala Gln Phe Arg Ser 
1               5                   10                  15      


Leu Asp 
        


<210>  120
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  120

Gly Gly Arg Arg Gly Gly Gly Ser 
1               5               


<210>  121
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  121

Leu Arg Gln Arg Asp Gly Glu Arg Pro 
1               5                   


<210>  122
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  122

Leu Arg Gln Lys Asp Gly Gly Gly Ser Glu Arg Pro 
1               5                   10          


<210>  123
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  123

Leu Arg Gln Lys Asp Gly Gly Gly Ser Gly Gly Gly Ser Glu Arg Pro 
1               5                   10                  15      


<210>  124
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage sequence by TEV protease


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa is any amino acid

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa = Gly or Ser

<400>  124

Glu Xaa Xaa Tyr Xaa Gln Xaa 
1               5           


<210>  125
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage sequence by TEV protease

<400>  125

Glu Asn Leu Tyr Phe Gln Gly 
1               5           


<210>  126
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage sequence by TEV protease

<400>  126

Glu Asn Leu Tyr Phe Gln Ser 
1               5           


<210>  127
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  127

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  128
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  128

Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  129
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  129

Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro 
1               5                   10                  


<210>  130
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  130

Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu 
1               5                   10                  15      


Glu Asn Pro Gly Pro 
            20      


<210>  131
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  131

Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  132
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  132

Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro 
1               5                   10              


<210>  133
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  133

Gly Ser Gly Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp 
1               5                   10                  15      


Val Glu Ser Asn Pro Gly Pro 
            20              


<210>  134
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  134

Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  135
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  135

Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 
1               5                   10                  


<210>  136
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  136

Gly Ser Gly Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala 
1               5                   10                  15      


Gly Asp Val Glu Ser Asn Pro Gly Pro 
            20                  25  


<210>  137
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  137

Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val 
1               5                   10                  15      


Glu Ser Asn Pro Gly Pro 
            20          


<210>  138
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  138

Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 
1               5                   10                  


<210>  139
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  139

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  140
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  140

Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  141
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  141

Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 
1               5                   10                  


<210>  142
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  142

Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly 
1               5                   10                  15      


Pro 
    


<210>  143
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  143

Gln Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  144
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  144

Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly 
1               5                   10                  15      


Asp Val Glu Ser Asn Pro Gly Pro 
            20                  


<210>  145
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  145

Val Thr Glu Leu Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro 
1               5                   10                  15      


Arg Pro Leu Leu Ala Ile His Pro Thr Glu Ala Arg His Lys Gln Lys 
            20                  25                  30          


Ile Val Ala Pro Val Lys Gln Thr 
        35                  40  


<210>  146
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  146

Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  147
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  147

Leu Leu Ala Ile His Pro Thr Glu Ala Arg His Lys Gln Lys Ile Val 
1               5                   10                  15      


Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly 
            20                  25                  30          


Asp Val Glu Ser Asn Pro Gly Pro 
        35                  40  


<210>  148
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  148

Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro Val Lys Gln Thr Leu 
1               5                   10                  15      


Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly 
            20                  25                  30          


Pro 
    


<210>  149
<211>  10
<212>  DNA
<213>  Unknown

<220>
<223>  Kozak sequence

<400>  149
gccrccatgg                                                              10


<210>  150
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  150

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


<210>  151
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  151

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  152
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  152

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser 
            20  


<210>  153
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  153

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser 
            20                  25  


<210>  154
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - PD-1 megaTAL polynucelotide section

<400>  154
ctggtggggc tgctccaggc atgcagatcc cacaggcgcc c                           41


<210>  155
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - PD-1 megaTAL polynucelotide section with deletion

<400>  155
ctggtggggc tgctccaggc atgcagccac aggcgccc                               38


<210>  156
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - PD-1 megaTAL polynucelotide section with deletion

<400>  156
ctggtggggc tgctccaggc atgcagcaca ggcgccc                                37


<210>  157
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - PD-1 megaTAL polynucelotide section with deletion

<400>  157
ctggtggggc tgctccaggc atgcacacag gcgccc                                 36


<210>  158
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - PD-1 megaTAL polynucelotide section with deletion

<400>  158
ctggtggggc tgctccacag gcgccc                                            26


<210>  159
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - PD-1 megaTAL polypeptide section

<400>  159

Met Gln Ile Pro Gln Ala Pro 
1               5           


<210>  160
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - PD-1 megaTAL polypeptide section with deletion

<400>  160

Met Gln Pro Gln Ala Pro 
1               5       


<210>  161
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - PD-1 megaTAL polypeptide section with deletion

<400>  161

Met Gln His Arg Arg Pro 
1               5       


<210>  162
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - PD-1 megaTAL polypeptide section with deletion

<400>  162

Met His Thr Gly Ala Leu 
1               5       


<210>  163
<211>  29
<212>  DNA
<213>  Homo sapiens

<400>  163
gtggggtatg tgtgtgtccg gggctgccg                                         29


<210>  164
<211>  21
<212>  DNA
<213>  Homo sapiens

<400>  164
aacaaaagcg gctctgagtc t                                                 21


