<110>    AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION

<120>    Methods for Producing Induced Pluripotent Stem Cell Using
         CRISPR/Cas Systems

<130>    PP-B2698

<160>    57

<170>    KoPatentIn 3.0

<210>    1
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    gRNA 1


<400>    1
gtcctgatat ttaatgaggc                                                     20


<210>    2
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    gRNA 2


<400>    2
gtcctaagac caccctgagc                                                     20


<210>    3
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    gRNA 3


<400>    3
gagttgatgg tttttagaac                                                     20


<210>    4
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    gRNA 4


<400>    4
gtaagagata catctaacat                                                     20


<210>    5
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    gRNA 5


<400>    5
gtttgtactt ataactaggc                                                     20


<210>    6
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    gRNA 6


<400>    6
gtgtatgaat atagatacat                                                     20


<210>    7
<211>    24
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    g1 Forward oligonucleotide


<400>    7
caccgtcctg atatttaatg aggc                                                24


<210>    8
<211>    24
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    g1 Reverse oligonucleotide


<400>    8
aaacgcctca ttaaatatca ggac                                                24


<210>    9
<211>    24
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    g2 Forward oligonucleotide


<400>    9
caccgtccta agaccaccct gagc                                                24


<210>    10
<211>    24
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    g2 Reverse oligonucleotide


<400>    10
aaacgctcag ggtggtctta ggac                                                24


<210>    11
<211>    24
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    g3 Forward oligonucleotide


<400>    11
caccgagttg atggttttta gaac                                                24


<210>    12
<211>    24
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    g3 Reverse oligonucleotide


<400>    12
aaacgttcta aaaaccatca actc                                                24


<210>    13
<211>    24
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    g4 Forward oligonucleotide


<400>    13
caccgtaaga gatacatcta acat                                                24


<210>    14
<211>    24
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    g4 Reverse oligonucleotide


<400>    14
aaacatgtta gatgtatctc ttac                                                24


<210>    15
<211>    24
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    g5 Forward oligonucleotide


<400>    15
caccgtttgt acttataact aggc                                                24


<210>    16
<211>    24
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    g5 Reverse oligonucleotide


<400>    16
aaacgcctag ttataagtac aaac                                                24


<210>    17
<211>    24
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    g6 Forward oligonucleotide


<400>    17
caccgtgtat gaatatagat acat                                                24


<210>    18
<211>    24
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    g6 Reverse oligonucleotide


<400>    18
aaacatgtat ctatattcat acac                                                24


<210>    19
<211>    18
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    U6 promoter Forward oligonucleotide


<400>    19
gagggcctat ttcccatg                                                       18


<210>    20
<211>    24
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    T7E1 assay First Forward primer


<400>    20
cttctgtcct catcattggt aacg                                                24


<210>    21
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    T7E1 assay First Reverse primer


<400>    21
acatctgaga aggcacctca c                                                   21


<210>    22
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    T7E1 assay Second Forward primer


<400>    22
ggcattggct tctttagggc                                                     20


<210>    23
<211>    24
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    T7E1 assay Second Reverse primer


<400>    23
gtgtcactta aaatagtagg tccc                                                24


<210>    24
<211>    22
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    5' Junction PCR Outer-FP primer


<400>    24
tccaataaaa taagcgggtg ac                                                  22


<210>    25
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    5' Junction PCR Donor-RP2 primer


<400>    25
ggcgccctgg tttacataag                                                     20


<210>    26
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    3' Junction PCR Donor-FP6 primer


<400>    26
gttcccatct caaggcacac                                                     20


<210>    27
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    3' Junction PCR Outer-RP primer


<400>    27
tccaccattc tcccactgac                                                     20


<210>    28
<211>    23
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    3' A (FC 1:3) Donor-FP primer


<400>    28
tcaaggtcaa aatcgtcaag agc                                                 23


<210>    29
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    3' A (FC 1:3) Outer-RP primer


<400>    29
tccaccattc tcccactgac                                                     20


<210>    30
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    3' B (FC 1:3) Donor-FP3 primer


<400>    30
tcctctcgtt gtgcatagtc g                                                   21


<210>    31
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    3' B (FC 1:3) Outer-RP primer


<400>    31
tccaccattc tcccactgac                                                     20


<210>    32
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    3' C (FC 1:3) Donor-FP5 primer


<400>    32
ccagctcgca gacctacatg                                                     20


<210>    33
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    3' C (FC 1:3) Outer-RP primer


<400>    33
tccaccattc tcccactgac                                                     20


<210>    34
<211>    23
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Donor D (FC 1:3) Donor-FP primer


<400>    34
tcaaggtcaa aatcgtcaag agc                                                 23


<210>    35
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Donor D (FC 1:3) Donor-RP4 primer


<400>    35
catgtgtgag aggggcagtg                                                     20


<210>    36
<211>    22
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    5' E (FUC 1:1) Outer-FP primer


<400>    36
tccaataaaa taagcgggtg ac                                                  22


<210>    37
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    5' E (FUC 1:1) Donor-RP3 primer


<400>    37
gtttgaatgc atgggagagc                                                     20


<210>    38
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    OCT4 Forward primer


<400>    38
ttgggctcga gaaggatgtg                                                     20


<210>    39
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    OCT4 Reverse primer


<400>    39
tcctctcgtt gtgcatagtc g                                                   21


<210>    40
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    SOX2 Forward primer


<400>    40
gccctgcagt acaactccat                                                     20


<210>    41
<211>    19
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    SOX2 Reverse primer


<400>    41
tgccctgctg cgagtagga                                                      19


<210>    42
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    KLF4 Forward primer


<400>    42
cgccgctcca ttaccaagag                                                     20


<210>    43
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    KLF4 Reverse primer


<400>    43
cacgatcgtc ttcccctctt                                                     20


<210>    44
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    GAPDH Forward primer


<400>    44
gtggacctga cctgccgtct                                                     20


<210>    45
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    GAPDH Reverse primer


<400>    45
ggaggagtgg gtgtcgctgt                                                     20


<210>    46
<211>    23
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Endo-OCT4 Forward primer


<400>    46
gacaggggga ggggaggagc tag                                                 23


<210>    47
<211>    25
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Endo-OCT4 Reverse primer


<400>    47
cttccctcca accagttgcc ccaaa                                               25


<210>    48
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Endo-SOX2 Forward primer


<400>    48
tggcgaacca tctctgtggt                                                     20


<210>    49
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Endo-SOX2 Reverse primer


<400>    49
ccaacggtgt caacctgcat                                                     20


<210>    50
<211>    26
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Endo-KLF4 Forward primer


<400>    50
acagtctgtt atgcactgtg gtttca                                              26


<210>    51
<211>    27
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Endo-KLF4 Reverse primer


<400>    51
catttgttct gcttaaggca tacttgg                                             27


<210>    52
<211>    19
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    c-MYC


<400>    52
ccagcagcga ctctgagga                                                      19


<210>    53
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    c-MYC Reverse primer


<400>    53
gagcctgcct cttttccaca g                                                   21


<210>    54
<211>    25
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    NANOG


<400>    54
caagaaacag aagaccagaa ctgtg                                               25


<210>    55
<211>    24
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    NANOG Reverse primer


<400>    55
ccattgctat tcttcggcca gttg                                                24


<210>    56
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    GAPDH


<400>    56
gtggacctga cctgccgtct                                                     20


<210>    57
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    GAPDH Reverse primer


<400>    57
ggaggagtgg gtgtcgctgt                                                     20
