<110>    SAMYANG CORPORATION

<120>    Phosphatase with high substrate specificity and use of the same

<130>    OPP20215039KR

<150>    KR 10-2020-0180253
<151>    2020-12-21

<160>    21

<170>    KoPatentIn 3.0

<210>    1
<211>    217
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CloA6PP


<400>    1
Met Asn Asn Tyr Lys Ala Val Phe Phe Asp Phe Asp Tyr Thr Leu Ala
  1               5                  10                  15 

Asp Ser Ser Lys Gly Val Ile Glu Cys Ile Asn Tyr Ala Leu Gln Lys
             20                  25                  30 

Met Gly Tyr Pro Lys Ser Ser Pro Glu Ser Ile Cys Arg Thr Ile Gly
         35                  40                  45 

Leu Thr Leu Thr Asp Ala Tyr Arg Gln Leu Ser Lys Asp Thr Ser Asp
     50                  55                  60 

Ser Asn Ala Ile Leu Phe Arg Gln His Phe Lys Glu Arg Ala Asp Val
 65                  70                  75                  80 

Val Met Cys Asp Ser Thr Ile Ile Tyr Asn Thr Val Glu Ser Val Leu
                 85                  90                  95 

Lys Arg Leu Lys Ser Met Asn Val Lys Val Gly Ile Val Ser Thr Lys
            100                 105                 110 

Tyr Arg Tyr Arg Ile Glu Asp Ile Leu Lys Arg Asp Gly Leu Leu Gln
        115                 120                 125 

Tyr Phe Asp Ile Ile Ile Gly Gly Glu Asp Val Val Ala His Lys Pro
    130                 135                 140 

Asp Pro Glu Gly Leu Leu Glu Ala Ile Ser Arg Leu Glu Cys Gln Lys
145                 150                 155                 160 

Lys Asp Thr Leu Tyr Val Gly Asp Ser Thr Val Asp Ala Lys Thr Ala
                165                 170                 175 

Glu Asn Ala Gly Val Asp Phe Ala Ala Val Leu Thr Gly Thr Thr Glu
            180                 185                 190 

Trp Asn Glu Phe Leu Lys Tyr Ser Ser Arg Ala Val Ile Lys Asp Leu
        195                 200                 205 

Ser Gly Leu Leu Asp Ile Phe Glu Glu
    210                 215        


<210>    2
<211>    654
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    CloA6PP


<400>    2
atgaacaact acaaagctgt tttcttcgat ttcgactata ccctggctga ctcttcgaaa         60

ggcgttatcg aatgcatcaa ctatgcattg cagaaaatgg gttacccaaa aagcagccca        120

gaaagcattt gtcgtaccat tggcctgaca ctgaccgatg cgtatcgcca gctgagcaaa        180

gacacctctg actctaacgc aatcctcttc cgtcagcact tcaaagaacg tgcagatgtg        240

gtcatgtgcg attcaaccat tatttataac actgtggaat ccgttctcaa acgtctgaaa        300

tcaatgaacg ttaaagttgg cattgtgtct actaaatacc gttaccgtat tgaggacatc        360

ctcaaacgtg atggtctgct gcagtacttt gacattatca tcggtggtga agatgtagtc        420

gctcacaaac cggatccgga aggcctgttg gaagcaatct cccgcttgga atgtcagaaa        480

aaagatactc tgtacgttgg ggattccacc gtggatgcca aaaccgcgga gaacgccggt        540

gtggatttcg cggcggttct gaccggtacc accgaatgga acgaattcct gaaatactcc        600

tcgcgtgcgg ttatcaaaga cctgagcggt ctgctggaca tcttcgaaga gtaa              654


<210>    3
<211>    214
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CbaA6PP


<400>    3
Met Arg Phe Pro Val Val Ile Phe Asp Phe Asp Tyr Thr Leu Ala Asp
  1               5                  10                  15 

Ser Ser Arg Gly Val Leu Glu Cys Ile Asn His Ala Phe Lys Gly Met
             20                  25                  30 

Gly Leu Pro Lys Val Val Ala Glu Asp Ala Gln Arg Thr Ile Gly Leu
         35                  40                  45 

Ser Leu Pro Asn Ile Leu Val Thr Leu Ala Gly Arg Glu His Glu Gly
     50                  55                  60 

Arg Gly Gly Glu Phe Gly Arg Leu Phe Val Glu Lys Ala Asn Glu Val
 65                  70                  75                  80 

Met Thr Asp Leu Thr Phe Val Phe Glu Glu Val Pro Glu Val Ile Arg
                 85                  90                  95 

Arg Leu Lys Asp Glu Gly Leu Thr Leu Gly Ile Val Ser Thr Lys Phe
            100                 105                 110 

Arg Arg Arg Ile Glu Glu Ile Leu Gly Arg Glu Asp Leu Leu Glu Pro
        115                 120                 125 

Phe Asp Val Ile Val Gly Gly Glu Asp Val Ser Arg His Lys Pro Asp
    130                 135                 140 

Pro Glu Gly Leu Leu Ala Ala Ile Glu Arg Leu Gly Gly Ser Pro Ser
145                 150                 155                 160 

Gly Ser Leu Tyr Val Gly Asp Ser Val Thr Asp Ala Glu Thr Ala Arg
                165                 170                 175 

Arg Ala Gly Val Pro Phe Ala Ala Val Leu Asn Gly Val Thr Pro Arg
            180                 185                 190 

Glu Ala Phe Lys Asp Tyr Pro Ala Tyr Lys Ile Leu Asp Asn Leu Gly
        195                 200                 205 

Glu Leu Ile Asp Gln Ser
    210                


<210>    4
<211>    225
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    TalA6PP


<400>    4
Met Arg Ala Leu Val Phe Asp Leu Asp Gly Thr Leu Val Asp Thr Val
  1               5                  10                  15 

Tyr Ala His Val Leu Ala Trp Gln Leu Ala Leu Glu Glu Ala Gly Leu
             20                  25                  30 

Pro Ile Asp Gly Trp Arg Ile His Arg Arg Ile Gly Met Ser Gly Gly
         35                  40                  45 

Leu Phe Thr Arg Ala Val Ala Arg Glu Ile Gly Arg Pro Leu Arg Pro
     50                  55                  60 

Asp Glu Ala Glu Arg Leu Arg Asp Arg His Ser Gln Leu Phe Arg Ala
 65                  70                  75                  80 

Leu Leu Pro Arg Arg Arg Pro Leu Pro Gly Ala Arg Glu Leu Leu Ala
                 85                  90                  95 

Thr Leu Arg Arg Ala Gly Val Pro Phe Ala Ile Ala Thr Ser Gly Arg
            100                 105                 110 

Arg Pro Glu Ile Asp Ala Ser Leu Glu Val Leu Gly Ile Asp His Glu
        115                 120                 125 

Val Ala Val Val Glu Arg Gly Asp Val Pro Arg Ala Lys Pro Glu Pro
    130                 135                 140 

Asp Leu Phe Leu Ala Ala Ala Glu Arg Leu Gly Ile Glu Ala Gly Ala
145                 150                 155                 160 

Cys Tyr Ala Val Gly Asp Ala Val Trp Asp Leu Leu Ala Ala Arg Arg
                165                 170                 175 

Ala Gly Met Leu Gly Ile Gly Leu Leu Ser Gly Gly Tyr Gly Glu Asp
            180                 185                 190 

Glu Leu Val Arg Thr Gly Ala Phe Arg Val Tyr Arg Asp Pro Ala Glu
        195                 200                 205 

Leu Leu Arg Ser Ile Asp Glu Leu Gly Ile Asp Leu Glu Thr Ala Ala
    210                 215                 220 

Glu
225


<210>    5
<211>    220
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    AbaA6PP


<400>    5
Met Pro Ala Leu Ile Phe Asp Leu Asp Gly Thr Leu Val Asp Thr Val
  1               5                  10                  15 

Tyr Ala His Val Phe Ala Trp Gln Arg Ala Leu Ala Glu Ala Gly Leu
             20                  25                  30 

Ala Val Asp Gly Trp Arg Ile His Arg Arg Ile Gly Met Ser Gly Gly
         35                  40                  45 

Leu Phe Thr Arg Ala Val Ala Arg Glu Leu Gly Arg Glu Ile Thr Ser
     50                  55                  60 

Asp Glu Ala Glu Ala Leu Gln Arg Arg His Gly Glu Leu Phe Gln Gln
 65                  70                  75                  80 

Phe Leu Pro Asn Arg Arg Ala Leu Pro Gly Ala Val Glu Leu Leu Arg
                 85                  90                  95 

Ala Leu Arg Ser Ala Gly Val Pro His Gly Ile Ala Thr Ser Gly Arg
            100                 105                 110 

Arg Pro Glu Ile Asp Arg Ser Leu Glu Val Leu Gly Val Gly Ala Glu
        115                 120                 125 

Thr Ile Val Val Asp Arg Gly Asp Val Gln Arg Ala Lys Pro Ala Pro
    130                 135                 140 

Asp Leu Phe Leu Asn Cys Gln Gln Arg Leu Gly Val Pro Val Glu Asp
145                 150                 155                 160 

Cys Tyr Val Val Gly Asp Ala Val Trp Asp Leu Leu Ala Ala Arg Arg
                165                 170                 175 

Ala Arg Met Leu Cys Ile Gly Leu Leu Ser Gly Gly Tyr Gly Asp Asp
            180                 185                 190 

Glu Leu Thr Arg Val Gly Ala Phe Arg Val Tyr Arg Asp Ala Ala Glu
        195                 200                 205 

Leu His Ala Ser Leu Asp Glu Leu Gly Val Leu Pro
    210                 215                 220


<210>    6
<211>    224
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    ChbA6PP


<400>    6
Met Thr Pro Val Leu Leu Phe Asp Leu Asp Gly Thr Leu Val Asp Ser
  1               5                  10                  15 

Val Tyr Gln His Val Leu Ala Trp Arg Glu Val Leu Glu Thr Met Ser
             20                  25                  30 

Ile Gln Leu Ser Val Trp Lys Ile His Arg Arg Ile Gly Met Ser Gly
         35                  40                  45 

Gly Leu Phe Val Asn Ala Leu Leu Arg Glu Thr Gly Ser Arg Leu Asp
     50                  55                  60 

Pro Gln Ala Ile Glu Arg Leu Arg Gln Leu His Val Glu Ala Tyr Leu
 65                  70                  75                  80 

Arg Arg Phe Asp Gln Val Arg Pro Leu Pro Gly Ala Ser Glu Leu Leu
                 85                  90                  95 

Ala Arg Leu Thr Ala Ala Ala Val Pro Trp Ala Ile Ala Thr Ser Gly
            100                 105                 110 

Ser Leu Arg Thr Ala Arg Pro Ala Val Asp Leu Leu Gly Val Pro Pro
        115                 120                 125 

Ser Ala Val Leu Val Thr Arg Glu Asp Val Ala Arg Ala Lys Pro Asp
    130                 135                 140 

Pro Asp Leu Phe Leu Ala Ala Ala Thr Arg Leu Arg Ile Ser Ile Thr
145                 150                 155                 160 

Asp Ala Phe Val Val Gly Asp Ser Val Trp Asp Leu Leu Ala Ala Gln
                165                 170                 175 

Arg Ala Arg Ala Leu Gly Val Gly Val Leu Ser Gly Gly Tyr Gly Arg
            180                 185                 190 

Glu Glu Leu Gln Gln Ala Gly Ala Tyr Arg Val Tyr Asp Asp Pro Ala
        195                 200                 205 

Asp Leu Leu Arg His Leu Asp Glu Val Gly Val Arg Glu Pro Ser Ser
    210                 215                 220 




<210>    7
<211>    38
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Forward primer in CloA6PP


<400>    7
aaggagatat acatatgaac aactacaaag ctgttttc                                 38


<210>    8
<211>    37
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Reverse primer in CloA6PP


<400>    8
ggtggtggtg ctcgagctct tcgaagatgt ccagcag                                  37


<210>    9
<211>    35
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Forward primer in CbaA6PP


<400>    9
aaggagatat acatatgcgt ttcccggtcg ttatc                                    35


<210>    10
<211>    37
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Reverse primer in CbaA6PP


<400>    10
ggtggtggtg ctcgagagac tgatcaatca gttcacc                                  37


<210>    11
<211>    36
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Forward primer in TalA6PP


<400>    11
aaggagatat acatatgcgt gcactggtat tcgacc                                   36


<210>    12
<211>    35
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Reverse primer in TalA6PP


<400>    12
ggtggtggtg ctcgagttcg gctgcagttt ccagg                                    35


<210>    13
<211>    38
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Forward primer in AbaA6PP


<400>    13
aaggagatat acatatgcca gcgttaatct ttgatctg                                 38


<210>    14
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Reverse primer in AbaA6PP


<400>    14
ggtggtggtg ctcgagtggc agcacgccca gctc                                     34


<210>    15
<211>    35
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Forward primer in ChbA6PP


<400>    15
aaggagatat acatatgaca ccggtacttt tattc                                    35


<210>    16
<211>    36
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Reverse primer in ChbA6PP


<400>    16
ggtggtggtg ctcgagagat gaaggttcac gaacac                                   36


<210>    17
<211>    227
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    fructose 6-phosphate 3-epimerase


<400>    17
Met Lys Tyr Gly Phe Ala Pro Ser Leu Met Cys Met Asn Ile Leu Lys
  1               5                  10                  15 

Leu Glu Glu Gln Ile Lys Ile Leu Asn Lys Lys Ala Asp Phe Tyr His
             20                  25                  30 

Val Asp Ile Met Asp Gly His Tyr Val Lys Asn Ile Thr Leu Ser Pro
         35                  40                  45 

Phe Phe Ile Gln Gln Ile Arg Pro Leu Ala Thr Ile Pro Ile Asp Ala
     50                  55                  60 

His Leu Met Val Glu Asn Pro Asn Asp Phe Leu Asp Glu Leu Lys Lys
 65                  70                  75                  80 

Ala Gly Thr Asp Tyr Val Ser Leu His Val Glu Thr Ile Asn Lys Asp
                 85                  90                  95 

Val Phe Arg Ile Thr Asn Lys Ile Lys Ser Leu Gly Met Lys Val Gly
            100                 105                 110 

Val Val Leu Asn Pro Ala Thr Pro Ile Asp Glu Ile Lys His Tyr Ile
        115                 120                 125 

Asn Arg Leu Asp Lys Ile Thr Val Met Thr Val Asp Pro Gly Phe Ala
    130                 135                 140 

Gly Gln Ala Phe Ile Pro Glu Met Leu Asp Lys Ile Arg Glu Leu Lys
145                 150                 155                 160 

Glu Ile Lys Glu Lys Glu Gly Tyr Lys Tyr Ile Ile Glu Ile Asp Gly
                165                 170                 175 

Ser Cys Asn Glu Lys Thr Phe Ala Gln Leu Lys Glu Ala Gly Ala Glu
            180                 185                 190 

Val Phe Val Val Gly Ser Ser Gly Leu Phe Asn Lys Asp Glu Asp Leu
        195                 200                 205 

Glu Lys Ala Trp Glu Met Met Met Ser Asp Phe Asn Tyr Ser Leu Ser
    210                 215                 220 

Tyr Lys Glu
225        


<210>    18
<211>    684
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    fructose 6-phosphate 3-epimerase


<400>    18
atgaaatacg gcttcgcacc gtccctgatg tgcatgaaca tcctgaaact ggaagaacaa         60

atcaaaattc tgaacaaaaa agcggatttc taccacgtag acattatgga tgggcactat        120

gttaaaaaca tcacgctgtc tccgttcttt atccaacaga tccgcccgct ggcgaccatt        180

ccaatcgacg cgcatctgat ggtcgagaac ccgaacgact ttctggacga actgaaaaaa        240

gctggtactg actacgtgtc tctgcacgta gaaaccatta acaaagatgt tttccgcatc        300

acgaacaaaa tcaaatcgtt aggtatgaaa gttggcgtgg ttctgaaccc tgcgactccg        360

atcgacgaaa tcaaacatta catcaaccgt ctggacaaaa tcactgttat gactgttgac        420

cctggtttcg ctggccaagc gttcatcccg gaaatgctgg acaaaatccg tgaactcaag        480

gaaatcaaag aaaaagaggg ttacaaatac attattgaaa tcgatggcag ctgcaacgaa        540

aagactttcg cccaactgaa agaagcgggt gcggaagttt tcgtagtcgg ctctagcggc        600

ctgtttaaca aagatgaaga cctggaaaaa gcctgggaaa tgatgatgtc cgacttcaat        660

tacagcctga gctacaaaga atag                                               684


<210>    19
<211>    225
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Ribulose-phosphate 3-epimerase (RuFP3E)


<400>    19
Met Lys Val Glu Phe Ser Pro Ser Leu Met Cys Met Asp Phe Leu Lys
  1               5                  10                  15 

Met Glu Glu Gln Phe Asn Ile Leu Asn Asp Lys Val Asp Met Tyr His
             20                  25                  30 

Ile Asp Ile Met Asp Gly His Phe Cys Lys Asn Ile Thr Leu Ser Pro
         35                  40                  45 

Asp Leu Val Lys Thr Phe Ser Lys Val Ala Lys Lys Pro Met Asp Val
     50                  55                  60 

His Leu Met Thr Thr Asn Pro Thr Asp Trp Ile Glu Lys Val Ala Ala
 65                  70                  75                  80 

Ala Gly Ala Glu Tyr Ile Ser Pro His Ala Glu Thr Ile Asn Gly Asp
                 85                  90                  95 

Ala Phe Arg Val Met Asn Met Ile Lys Ser Leu Gly Cys Lys Thr Gly
            100                 105                 110 

Val Val Leu Asn Pro Ala Thr Pro Leu Ser Tyr Val Lys His Tyr Leu
        115                 120                 125 

Asn Arg Ile Asp Leu Leu Thr Ile Met Thr Val Asp Val Gly Phe Ala
    130                 135                 140 

Gly Gln Pro Phe Ile Glu Glu Met Leu Asp Lys Ile Lys Glu Ala Lys
145                 150                 155                 160 

Glu Leu Lys Glu Lys Tyr Gly Tyr Thr Tyr Lys Ile Gln Ile Asp Gly
                165                 170                 175 

Ser Cys Asn Pro Lys Thr Tyr Lys Arg Met Tyr Asp Ala Gly Ala Glu
            180                 185                 190 

Val Leu Ile Val Gly Ser Ser Gly Leu Phe Gly Leu Asn Glu Asp Leu
        195                 200                 205 

Asn Lys Ala Cys Asp Gln Met Phe Glu Asp Phe Asn Arg Ala Ile Ala
    210                 215                 220 

Glu
225


<210>    20
<211>    225
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Ribulose-phosphate 3-epimerase (CDFP3E)


<400>    20
Met Lys Pro Met Phe Ala Pro Ser Leu Met Cys Ala Asn Phe Leu Asp
  1               5                  10                  15 

Leu Lys Asn Gln Ile Glu Ile Leu Asn Glu Arg Ala Asp Ile Phe His
             20                  25                  30 

Val Asp Ile Met Asp Gly His Tyr Val Lys Asn Phe Ser Leu Ser Pro
         35                  40                  45 

Ala Met Met Glu Gln Leu Lys Thr Ile Thr Lys Ile Pro Met Asp Ala
     50                  55                  60 

His Leu Met Val Glu Asn Pro Ala Asp Phe Leu Glu Gly Ile Ala Lys
 65                  70                  75                  80 

Ala Gly Ala Thr Tyr Ile Ser Pro His Ala Glu Thr Ile Asn Lys Asp
                 85                  90                  95 

Ala Phe Arg Ile Met Arg Thr Ile Lys Ala Leu Gly Cys Lys Thr Gly
            100                 105                 110 

Val Val Leu Asn Pro Ala Thr Pro Val Glu Tyr Ile Lys His Tyr Leu
        115                 120                 125 

Gly Met Leu Asp Lys Ile Thr Ile Leu Thr Val Asp Ala Gly Phe Ala
    130                 135                 140 

Gly Gln Thr Phe Ile Glu Glu Met Leu Asp Lys Ile Glu Glu Val Lys
145                 150                 155                 160 

Arg Leu Arg Glu Glu Asn Gly Tyr Ser Tyr Leu Ile Glu Val Asp Gly
                165                 170                 175 

Ser Cys Asn Glu Lys Thr Phe Lys Lys Leu Ala Glu Ala Gly Thr Glu
            180                 185                 190 

Val Phe Ile Val Gly Ser Ser Gly Leu Phe Asn Leu Asp Ala Asp Leu
        195                 200                 205 

Lys Val Ser Trp Asp Lys Met Met Asn Met Phe Asn Lys Cys Ile Asn
    210                 215                 220 

Asn
225


<210>    21
<211>    228
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Ribulose-phosphate 3-epimerase (PkFP3E)


<400>    21
Met Lys Lys His Leu Phe Ser Pro Ser Leu Met Cys Met Asn Leu Met
  1               5                  10                  15 

Asp Ile Glu Asn Gln Leu Lys Val Leu Asn Gln Arg Ala Asp Met Val
             20                  25                  30 

His Ile Asp Ile Met Asp Gly His Tyr Val Lys Asn Ile Thr Leu Ser
         35                  40                  45 

Pro Tyr Phe Met Glu Gln Ile Arg Glu Ser Leu Thr Ile Pro Met Asp
     50                  55                  60 

Val His Leu Met Val Glu Lys Pro Ser Glu Phe Ile Lys Met Ile Ile
 65                  70                  75                  80 

Asp Ala Gly Ala Thr Tyr Ile Ser Pro His Ala Glu Val Ile Asn Ser
                 85                  90                  95 

Glu Ala Phe Arg Met Ile Asp Thr Ile Lys Ser Ala Gly Cys Lys Ala
            100                 105                 110 

Gly Ile Ala Leu Asn Pro Ala Thr Pro Leu Ser His Ile Gln His Tyr
        115                 120                 125 

Ile His His Leu Asp Lys Ile Thr Phe Met Ser Val Asp Pro Gly Phe
    130                 135                 140 

Ala Gly Gln Lys Phe Ile Pro Glu Val Leu Asp Lys Ile Arg Glu Ala
145                 150                 155                 160 

Arg Ala Leu Lys Glu Lys His Gly Tyr Asn Tyr Leu Ile Glu Ile Asp
                165                 170                 175 

Gly Ser Cys Asn Glu Lys Thr Phe Lys Ile Leu Glu Glu Ala Gly Val
            180                 185                 190 

Glu Val Phe Ile Val Gly Thr Ser Gly Leu Phe Asn Leu Asp Ser Asp
        195                 200                 205 

Leu Asn Lys Ala Trp Asp Gln Met Met Glu His Phe Asn Ser Gln Val
    210                 215                 220 

Asn Ala Glu Val
225            
