                         SEQUENCE LISTING

<110>  Intervet International BV
 
<120>  Multivalent HVT vector vaccine

<130>  25166

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  5498
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression cassette with IBDV-VP2 and NDV-F genes

<400>  1
aactccgccc gttttatgac tagaaccaat agtttttaat gccaaatgca ctgaaatccc       60

ctaatttgca aagccaaacg ccccctatgt gagtaatacg gggacttttt acccaatttc      120

ccaagcggaa agccccctaa tacactcata tggcatatga atcagcacgg tcatgcactc      180

taatggcggc ccatagggac tttccacata gggggcgttc accatttccc agcatagggg      240

tggtgactca atggccttta cccaagtaca ttgggtcaat gggaggtaag ccaatgggtt      300

tttcccatta ctggcaagca cactgagtca aatgggactt tccactgggt tttgcccaag      360

tacattgggt caatgggagg tgagccaatg ggaaaaaccc attgctgcca agtacactga      420

ctcaataggg actttccaat gggtttttcc attgttggca agcatataag gtcaatgtgg      480

gtgagtcaat agggactttc cattgtattc tgcccagtac ataaggtcaa tagggggtga      540

atcaacagga aagtcccatt ggagccaagt acactgcgtc aatagggact ttccattggg      600

ttttgcccag tacataaggt caatagggga tgagtcaatg ggaaaaaccc attggagcca      660

agtacactga ctcaataggg actttccatt gggttttgcc cagtacataa ggtcaatagg      720

gggtgagtca acaggaaagt cccattggag ccaagtacat tgagtcaata gggactttcc      780

aatgggtttt gcccagtaca taaggtcaat gggaggtaag ccaatgggtt tttcccatta      840

ctggcacgta tactgagtca ttagggactt tccaatgggt tttgcccagt acataaggtc      900

aataggggtg aatcaacagg aaagtcccat tggagccaag tacactgagt caatagggac      960

tttccattgg gttttgccca gtacaaaagg tcaatagggg gtgagtcaat gggtttttcc     1020

cattattggc acgtacataa ggtcaatagg ggtgagtcat tgggtttttc cagccaattt     1080

aattaaaacg ccatgtactt tcccaccatt gacgtcaatg ggctattgaa actaatgcaa     1140

cgtgaccttt aaacggtact ttcccatagc tgattaatgg gaaagtaccg ttctcgagcc     1200

aatacacgtc aatgggaagt gaaagggcag ccaaaacgta acaccgcccc ggttttcccc     1260

tggaaattcc atattggcac gcattctatt ggctgagctg cgttctacgt gggtataaga     1320

ggcgcgacca gcgtcggtac cgtcgcagtc ttcggtctga ccaccgtaga acgcagagct     1380

cctcgctgca ggcggccgct ctagaactcg tcgatcgcag cgatgacaaa cctgcaagat     1440

caaacccaac agattgttcc gttcatacgg agccttctga tgccaacaac cggaccggcg     1500

tccattccgg acgacaccct ggagaagcac actctcaggt cagagacctc gacctacaat     1560

ttgactgtgg gggacacagg gtcagggcta attgtctttt tccctggatt ccctggctca     1620

attgtgggtg ctcactacac actgcagagc aatgggaact acaagttcga tcagatgctc     1680

ctgactgccc agaacctacc ggccagctac aactactgca gactagtgag tcggagtctc     1740

acagtgaggt caagcacact ccctggtggc gtttatgcac taaacggcac cataaacgcc     1800

gtgaccttcc aaggaagcct gagtgaactg acagatgtta gctacaatgg gttgatgtct     1860

gcaacagcca acatcaacga caaaattggg aatgtcctgg taggggaagg ggtcactgtc     1920

ctcagcctac ccacatcata tgatcttggg tatgtgaggc ttggtgaccc cattcccgct     1980

atagggcttg acccaaaaat ggtagctaca tgcgacagca gtgacaggcc cagagtctac     2040

accataactg cagccgatga ttaccaattc tcatcacagt accaaccagg tggggtaaca     2100

atcacactgt tctcagccaa cattgatgct atcacaagcc tcagcattgg gggagagctc     2160

gtgtttcaaa caagcgtcca aggccttgta ctgggcgcca ccatctacct tataggcttt     2220

gatgggactg cggtaatcac cagagctgta gccgcagata atgggctgac ggccggcacc     2280

gacaatctta tgccattcaa tcttgtcatt ccaaccaatg agataaccca gccaatcaca     2340

tccatcaaac tggagatagt gacctccaaa agtggtggtc aggcagggga tcagatgtca     2400

tggtcggcaa gtgggagcct agcagtgacg atccatggtg gcaactatcc aggggccctc     2460

cgtcccgtca cactagtagc ctacgaaaga gtggcaacag gatccgtcgt tacggtcgct     2520

ggggtgagta acttcgagct gattccaaat cctgaactag caaagaacct ggttacagaa     2580

tacggccgat ttgacccagg agccatgaac tacacaaaat tgatactgag tgagagggac     2640

cgtcttggca tcaagaccgt ctggccaaca agggagtaca ctgattttcg tgagtacttc     2700

atggaggtgg ccgacctcaa ctctcccctg aagattgcag gagcatttgg cttcaaagac     2760

ataatccggg ctataaggag gtaagcttga tctagagcgg ccgcggggat ccagacatga     2820

taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta     2880

tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag     2940

ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt     3000

tttcggatcc tctagagtcg acaattattt catttaataa catatagccc aaagacctct     3060

atgaacattt agtttcccgt atactcaacg gcgcgtgtac acacaagggc gaattccaca     3120

gtggatatca agcttaatta agtaccgagc tcgaattggc gcgccaggtc aattccctgg     3180

cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta     3240

gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg     3300

tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg     3360

caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg     3420

ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag     3480

atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggcgcgccgg     3540

atccatgggc cccagacctt ctaccaagaa cccagtacct atgatgctga ctgtccgagt     3600

cgcgctggta ctgagttgca tctgtccggc aaactccatt gatggcaggc ctcttgcggc     3660

tgcaggaatt gtggttacag gagacaaagc cgtcaacata tacacctcat cccagacagg     3720

atcaatcata gttaagctcc tcccgaatct gcccaaggat aaggaggcat gtgcgaaagc     3780

ccccttggat gcatacaaca ggacattgac cactttgctc accccccttg gtgactctat     3840

ccgtaggata caagagtctg tgactacatc tggagggggg agacaggggc gccttatagg     3900

cgccattatt ggcggtgtgg ctcttggggt tgcaactgcc gcacaaataa cagcggccgc     3960

agctctgata caagccaaac aaaatgctgc caacatcctc cgacttaaag agagcattgc     4020

cgcaaccaat gaggctgtgc atgaggtcac tgacggatta tcgcaactag cagtggcagt     4080

tgggaagatg cagcagtttg ttaatgacca atttaataaa acagctcagg aattagactg     4140

catcaaaatt gcacagcaag ttggtgtaga gctcaacctg tacctaaccg aattgactac     4200

agtattcgga ccacaaatca cttcacctgc tttaaacaag ctgactattc aggcacttta     4260

caatctagct ggtggaaata tggattactt attgactaag ttaggtgtag ggaacaatca     4320

actcagctca ttaatcggta gcggcttaat caccggtaac cctattctat acgactcaca     4380

gactcaactc ttgggtatac aggtaactct accttcagtc gggaacctaa ataatatgcg     4440

tgccacctac ttggaaacct tatccgtaag cacaaccagg ggatttgcct cggcacttgt     4500

cccaaaagtg gtgacacagg tcggttctgt gatagaagaa cttgacacct catactgtat     4560

agaaactgac ttagatttat attgtacaag aatagtaacg ttccctatgt cccctggtat     4620

ttattcctgc ttgagcggca atacgtcggc ctgtatgtac tcaaagaccg aaggcgcact     4680

tactacacca tacatgacta tcaaaggttc agtcatcgcc aactgcaaga tgacaacatg     4740

tagatgtgta aaccccccgg gtatcatatc gcaaaactat ggagaagccg tgtctctaat     4800

agataaacaa tcatgcaatg ttttatcctt aggcgggata actttaaggc tcagtgggga     4860

attcgatgta acttatcaga agaatatctc aatacaagat tctcaagtaa taataacagg     4920

caatcttgat atctcaactg agcttgggaa tgtcaacaac tcgatcagta atgctttgaa     4980

taagttagag gaaagcaaca gaaaactaga caaagtcaat gtcaaactga ctagcacatc     5040

tgctctcatt acctatatcg ttttgactat catatctctt gtttttggta tacttagccc     5100

gattctagca tgctacctaa tgtacaagca aaaggcgcaa caaaagacct tattatggct     5160

tgggaataat actctagatc agatgagagc cactacaaaa atgtgaggat ctctcgagga     5220

attctagatc ccacgtcact attgtatact ctatattata ctctatgtta tactctgtaa     5280

tcctactcaa taaacgtgtc acgcctgtga aaccgtacta agtctcccgt gtcttcttat     5340

caccatcagg tgacatcctc gcccaggctg tcaatcatgc cggtatcgat tccagtagca     5400

ccggccccac gctgacaacc cactcttgca gcgttagcag cgcccctctt aacaagccga     5460

cccccaccag cgtcgcggtt actaacactc ctctcccc                             5498


<210>  2
<211>  3157
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expression cassette with ILTV gD and gI genes

<400>  2
aagcttaatt aacgtcgacg gcagagtcgc agacgcccct attggacgtc aaaattgtag       60

aggtgaagtt ttcaaacgat ggcgaagtaa cggcgacttg cgtttccacc gtcaaatctc      120

cctatagggt agaaactaat tggaaagtag acctcgtaga tgtaatggat gaaatttctg      180

ggaacagtcc cgccggggtt tttaacagta atgagaaatg gcagaaacag ctgtactaca      240

gagtaaccga tggaagaaca tcggtccagc taatgtgcct gtcgtgcacg agccattctc      300

cggaacctta ctgtcttttc gacacgtctc ttatagcgag ggaaaaagat atcgcgccag      360

agttatactt tacctctgat ccgcaaacgg catactgcac aataactctg ccgtccggcg      420

ttgttccgag attcgaatgg agccttaata atgtttcact gccggaatat ttgacggcca      480

cgaccgttgt ttcgcatacc gctggccaaa gtacagtgtg gaagagcagc gcgagagcag      540

gcgaggcgtg gatttctggc cggggaggca atatatacga atgcaccgtc ctcatctcag      600

acggcactcg cgttactacg cgaaaggaga ggtgcttaac aaacacatgg attgcggtgg      660

aaaacggtgc tgctcaggcg cagctgtatt cactcttttc tggacttgtg tcaggattat      720

gcgggagcat atctgctttg tacgcaacgc tatggaccgc catttatttt tgaggaatgc      780

tttttggact atcgtactgc tttcttcctt cgctagccag agcaccgccg ccgtcacgta      840

cgactacatt ttaggccgtc gcgcgctcga cgcgctaacc ataccggcgg ttggcccgta      900

taacagatac ctcactaggg tatcaagagg ctgcgacgtt gtcgagctca acccgatttc      960

taacgtggac gacatgatat cggcggccaa agaaaaagag aaggggggcc ctttcgaggc     1020

ctccgtcgtc tggttctacg tgattaaggg cgacgacggc gaggacaagt actgtccaat     1080

ctatagaaaa gagtacaggg aatgtggcga cgtacaactg ctatctgaat gcgccgttca     1140

atctgcacag atgtgggcag tggactatgt tcctagcacc cttgtatcgc gaaatggcgc     1200

gggactgact atattctccc ccactgctgc gctctctggc caatacttgc tgaccctgaa     1260

aatcgggaga tttgcgcaaa cagctctcgt aactctagaa gttaacgatc gctgtttaaa     1320

gatcgggtcg cagcttaact ttttaccgtc gaaatgctgg acaacagaac agtatcagac     1380

tggatttcaa ggcgaacacc tttatccgat cgcagacacc aatacacgac acgcggacga     1440

cgtatatcgg ggatacgaag atattctgca gcgctggaat aatttgctga ggaaaaagaa     1500

tcctagcgcg ccagaccctc gtccagatag cgtcccgcaa gaaattcccg ctgtaaccaa     1560

gaaagcggaa gggcgcaccc cggacgcaga aagcagcgaa aagaaggccc ctccagaaga     1620

ctcggaggac gacatgcagg cagaggcttc tggagaaaat cctgccgccc tccccgaaga     1680

cgacgaagtc cccgaggaca ccgagcacga tgatccaaac tcggatcctg actattacaa     1740

tgacatgccc gccgtgatcc cggtggagga gactactaaa agttctaatg ccgtctccat     1800

gcccatattc gcggcgttcg tagcctgcgc ggtcgcgctc gtggggctac tggtttggag     1860

catcgtaaaa tgcgcgcgta gctaatcgag cctagaatag gtggtttctt cctacatgcc     1920

acgcctcacg ctcataatat aaatcacatg gaatagcata ccaatgccta ttcattggga     1980

cgttcgaaaa gcatggcatc gctacttgga actctggctc tccttgccgc gacgctcgca     2040

cccttcggcg cgatgggaat cgtgatcact ggaaatcacg tctccgccag gattgacgac     2100

gatcacatcg tgatcgtcgc gcctcgcccc gaagctacaa ttcaactgca gctatttttc     2160

atgcctggcc agagacccca caaaccctac tcaggaaccg tccgcgtcgc gtttcggtct     2220

gatataacaa accagtgcta ccaggaactt agcgaggagc gctttgaaaa ttgcactcat     2280

cgatcgtctt ctgtttttgt cggctgtaaa gtgaccgagt acacgttctc cgcctcgaac     2340

agactaaccg gacctccaca cccgtttaag ctcactatac gaaatcctcg tccgaacgac     2400

agcgggatgt tctacgtaat tgttcggcta gacgacacca aagaacccat tgacgtcttc     2460

gcgatccaac tatcggtgta tcaattcgcg aacaccgccg cgactcgcgg actctattcc     2520

aaggcttcgt gtcgcacctt cggattacct accgtccaac ttgaggccta tctcaggacc     2580

gaggaaagtt ggcgcaactg gcaagcgtac gttgccacgg aggccacgac gaccagcgcc     2640

gaggcgacaa ccccgacgcc cgtcactgca accagcgcct ccgaacttga agcggaacac     2700

tttacctttc cctggctaga aaatggcgtg gatcattacg aaccgacacc cgcaaacgaa     2760

aattcaaacg ttactgtccg tctcgggaca atgagcccta cgctaattgg ggtaaccgtg     2820

gctgccgtcg tgagcgcaac gatcggcctc gtcattgtaa tttccatcgt caccagaaac     2880

atgtgcaccc cgcaccgaaa attagacacg gtctcgcaag acgacgaaga acgttcccaa     2940

actagaaggg aatcgcgaaa atttggaccc atggttgcgt gcgaaataaa caagggggct     3000

gaccaggata gtgaacttgt ggaactggtt gcgattgtta acccgtctgc gctaagctcg     3060

cccgactcaa taaaaatgtg aagatctctt aattaacaat aaacatagca tacgttatga     3120

catggtctac cgcgtcttat atggggacga caagctt                              3157


<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  guide RNA for insertion between UL44 and UL45 genes of HVT

<400>  3
acatcgggac gtacatcatg                                                   20


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  guide RNA for insertion between UL45 and UL46 genes of HVT

<400>  4
ctaacggtta ctgtgtttta                                                   20


