                         SEQUENCE LISTING

<110>  Plant Bioscience Limited
 
<120>  Methods and Compositions

<130>  P137731WO

<150>  GB2020623.1
<151>  2020-12-24

<150>  GB2116554.3
<151>  2021-11-17

<160>  86    

<170>  PatentIn version 3.5

<210>  1
<211>  1374
<212>  DNA
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  A nucleic acid molecule comprising a nucleotide which encodes the
       enzyme according to SEQ ID NO 2.

<400>  1
atggagaatg ggagagttta caaatcccat gtcgtggtgc tcgcatttca cgggcaaggc       60

catattgttc cgttaatcca attatccaga cgattggcct ggaaaggcat caaaatcaca      120

tttgctacaa cacattcttg caccaaggcc attcaaacag gaagtgattc aatttcactt      180

ttatcaattt atgatgacat aactgatggt gggtttcaag gagaaggagg attcaagggc      240

ttccttcaga gatttgaagc cagtaccaca aggatcttac acgaattcgt caagaatcat      300

gaaaactcaa agaacccagt aaaatgctta atatatgatg ctaacttaat atgggctctg      360

gaaatggcaa agcaattggg tattgctact gctgcatttg tgtttccttc ttgggctgcc      420

attgccacct actatccctt ttatttagag gtgtatgcgg atcagcagat aaagaaggta      480

gatcctttca caatgcctga cttacctcca caacttggac ttccaaatat ggcatctctc      540

ggttcagatt cgggtcaaca ctcccccata ctcaaactca tgttgcaaca gttagaaaat      600

tttgggaaag ctgactggat cctgtctcac gcatttgaac agtttgaaca agaggtactt      660

gactggatga gaaatatcag cccagtaaca acaattggtc caactctgcc atctgtttat      720

cttgatggta ggctaaaaga tgacacagat tacggttaca atttgtacaa gccagatagt      780

gatacctgca tgaagtggct agacactaag gaaactgaat cagtggttta tatatcattt      840

ggcagtgttg cagatttgat cccagaacag atgacagaaa taacaaactc cctgaagaaa      900

atgagcagca actttctgtg ggtggtgaag gaaactgaaa aaaacaacct ccctagcagc      960

tttgttgagg agacaaaaga aaagggattg gtagtgactt ggtgccccca gttgaaggtg     1020

ttgtctcatc ctgcagtggg ttgtttcatt acacactgtg gaacaaattc catatttgag     1080

tcagtatgct ttgcagtgcc aatggtggga atgccacagt tttgtgatca aatgcctaat     1140

gcatatttca tggagaaggt ctggaaagta ggtgttaggc caagtttgga tgacaatggt     1200

gtcgtcactg gagaagaaat tgagcgatgt ataaaagtag ttaccgaagg agagagtggg     1260

caagagatta agaagaaact tgtgcagtgg aaagagcttg caaaagaggc agtggacgag     1320

ggtggaagtt cagataagca cattgatgaa ttcattgctg gaatcacaac ttga           1374


<210>  2
<211>  457
<212>  PRT
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  A fucosyltransferase enzyme capable of transferring 
       beta-d-fucopyranose to the C-28 position of Quillaic acid 
       (Qs-28-O-FucT).

<400>  2

Met Glu Asn Gly Arg Val Tyr Lys Ser His Val Val Val Leu Ala Phe 
1               5                   10                  15      


His Gly Gln Gly His Ile Val Pro Leu Ile Gln Leu Ser Arg Arg Leu 
            20                  25                  30          


Ala Trp Lys Gly Ile Lys Ile Thr Phe Ala Thr Thr His Ser Cys Thr 
        35                  40                  45              


Lys Ala Ile Gln Thr Gly Ser Asp Ser Ile Ser Leu Leu Ser Ile Tyr 
    50                  55                  60                  


Asp Asp Ile Thr Asp Gly Gly Phe Gln Gly Glu Gly Gly Phe Lys Gly 
65                  70                  75                  80  


Phe Leu Gln Arg Phe Glu Ala Ser Thr Thr Arg Ile Leu His Glu Phe 
                85                  90                  95      


Val Lys Asn His Glu Asn Ser Lys Asn Pro Val Lys Cys Leu Ile Tyr 
            100                 105                 110         


Asp Ala Asn Leu Ile Trp Ala Leu Glu Met Ala Lys Gln Leu Gly Ile 
        115                 120                 125             


Ala Thr Ala Ala Phe Val Phe Pro Ser Trp Ala Ala Ile Ala Thr Tyr 
    130                 135                 140                 


Tyr Pro Phe Tyr Leu Glu Val Tyr Ala Asp Gln Gln Ile Lys Lys Val 
145                 150                 155                 160 


Asp Pro Phe Thr Met Pro Asp Leu Pro Pro Gln Leu Gly Leu Pro Asn 
                165                 170                 175     


Met Ala Ser Leu Gly Ser Asp Ser Gly Gln His Ser Pro Ile Leu Lys 
            180                 185                 190         


Leu Met Leu Gln Gln Leu Glu Asn Phe Gly Lys Ala Asp Trp Ile Leu 
        195                 200                 205             


Ser His Ala Phe Glu Gln Phe Glu Gln Glu Val Leu Asp Trp Met Arg 
    210                 215                 220                 


Asn Ile Ser Pro Val Thr Thr Ile Gly Pro Thr Leu Pro Ser Val Tyr 
225                 230                 235                 240 


Leu Asp Gly Arg Leu Lys Asp Asp Thr Asp Tyr Gly Tyr Asn Leu Tyr 
                245                 250                 255     


Lys Pro Asp Ser Asp Thr Cys Met Lys Trp Leu Asp Thr Lys Glu Thr 
            260                 265                 270         


Glu Ser Val Val Tyr Ile Ser Phe Gly Ser Val Ala Asp Leu Ile Pro 
        275                 280                 285             


Glu Gln Met Thr Glu Ile Thr Asn Ser Leu Lys Lys Met Ser Ser Asn 
    290                 295                 300                 


Phe Leu Trp Val Val Lys Glu Thr Glu Lys Asn Asn Leu Pro Ser Ser 
305                 310                 315                 320 


Phe Val Glu Glu Thr Lys Glu Lys Gly Leu Val Val Thr Trp Cys Pro 
                325                 330                 335     


Gln Leu Lys Val Leu Ser His Pro Ala Val Gly Cys Phe Ile Thr His 
            340                 345                 350         


Cys Gly Thr Asn Ser Ile Phe Glu Ser Val Cys Phe Ala Val Pro Met 
        355                 360                 365             


Val Gly Met Pro Gln Phe Cys Asp Gln Met Pro Asn Ala Tyr Phe Met 
    370                 375                 380                 


Glu Lys Val Trp Lys Val Gly Val Arg Pro Ser Leu Asp Asp Asn Gly 
385                 390                 395                 400 


Val Val Thr Gly Glu Glu Ile Glu Arg Cys Ile Lys Val Val Thr Glu 
                405                 410                 415     


Gly Glu Ser Gly Gln Glu Ile Lys Lys Lys Leu Val Gln Trp Lys Glu 
            420                 425                 430         


Leu Ala Lys Glu Ala Val Asp Glu Gly Gly Ser Ser Asp Lys His Ile 
        435                 440                 445             


Asp Glu Phe Ile Ala Gly Ile Thr Thr 
    450                 455         


<210>  3
<211>  1422
<212>  DNA
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  A nucleic acid molecule comprising a nucleotide which encodes the
       enzyme according to SEQ ID NO 4.

<400>  3
atggcaaaaa ctgataagca gcttcacatc gccatgttcc catggctagc tatgggtcat       60

atattcccaa actttgagct cgctaagctc tttgctcaaa agggtcactc aattacttta      120

atctccaccc cacgaaacat cagtcgtctc cctcaaatcc ctacacattt agagcaattg      180

attaaattag tcagcttgcc tatattaccc aaacacaaag caaatctccc agagaatgca      240

gagtccacca tggacgttac ccccaataaa gtcccatacc ttaagatggc ctatgatggt      300

cttcaggagt cgttgactca attactgaaa tcttcagctc ccgattggat tctatatgac      360

tttgctgctg actggttacc accacttgtt cacagccttc aaatccgctg tgttttcttc      420

gtggtatccc ctgcgtggaa tctttgcttc tttgacactc ccaaaccaca gttgggcagt      480

gctgctgttt ttcgaacaaa gcctgaagac tatcttcgcc ctcccagttg ggttcctttc      540

cattcaaata ttgggctaaa gcttcacgag gtgaagaaaa tgtttgaagg ggtttcagat      600

aaagaaacag gggtcactgt aagttttaac ttcaacaaag cagtttcgag ctgtgacttg      660

ttttctttcc gcagctgcta tgaactcgaa tcagaatggc tgaacctggt ggaggatatt      720

tacaagaggc ctgtagttcc agtgggcgta attccaccct cttttcaagt cagaattgtg      780

aatgaagaag acaacaaacc agagtggtta aagatccaat cttggttaga taaacaagag      840

caaggatcgg tggtatacat agcatttggc agtgagctta agctgggcca acaagatctc      900

accgaattag ctcttggact tgagctttct gggttgccat tcttttgggc acttagaaag      960

cagcaagaca gctcatcagt agatttacca gatgggtttg aggaccgagt cagtgatcgt     1020

ggagttgttt gcagagactg ggtgccccaa cttaagatcc tagctcacgg gtcaattggg     1080

ggttatttga ctcactgtgg ttcaggttca gtgatagagg gacttcattt tgggcgtgtt     1140

cttgttatgc tgccctattt actagaccaa gcattatatg ctagagtatt ggaggagaaa     1200

aagctggggg ttgagatacc aaggaacgaa caagatgggt cttttactag gagctcagtg     1260

gccaagtctg tgaagttggc catagtggat gaggggggaa gtatttacag ggacaaagcc     1320

aaagagatgg gcttggtatt cagtgacaaa gatcgtcatg aacaatacat tgagaatttc     1380

cttcaacacc ttcaacacaa aagggaacct ttccaaattt aa                        1422


<210>  4
<211>  473
<212>  PRT
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  A rhamnosyltransferase enzyme, capable of transferring 
       alpha-1,2-l-rhamnopyranose to QA-F (Qs-28-O-RhaT).

<400>  4

Met Ala Lys Thr Asp Lys Gln Leu His Ile Ala Met Phe Pro Trp Leu 
1               5                   10                  15      


Ala Met Gly His Ile Phe Pro Asn Phe Glu Leu Ala Lys Leu Phe Ala 
            20                  25                  30          


Gln Lys Gly His Ser Ile Thr Leu Ile Ser Thr Pro Arg Asn Ile Ser 
        35                  40                  45              


Arg Leu Pro Gln Ile Pro Thr His Leu Glu Gln Leu Ile Lys Leu Val 
    50                  55                  60                  


Ser Leu Pro Ile Leu Pro Lys His Lys Ala Asn Leu Pro Glu Asn Ala 
65                  70                  75                  80  


Glu Ser Thr Met Asp Val Thr Pro Asn Lys Val Pro Tyr Leu Lys Met 
                85                  90                  95      


Ala Tyr Asp Gly Leu Gln Glu Ser Leu Thr Gln Leu Leu Lys Ser Ser 
            100                 105                 110         


Ala Pro Asp Trp Ile Leu Tyr Asp Phe Ala Ala Asp Trp Leu Pro Pro 
        115                 120                 125             


Leu Val His Ser Leu Gln Ile Arg Cys Val Phe Phe Val Val Ser Pro 
    130                 135                 140                 


Ala Trp Asn Leu Cys Phe Phe Asp Thr Pro Lys Pro Gln Leu Gly Ser 
145                 150                 155                 160 


Ala Ala Val Phe Arg Thr Lys Pro Glu Asp Tyr Leu Arg Pro Pro Ser 
                165                 170                 175     


Trp Val Pro Phe His Ser Asn Ile Gly Leu Lys Leu His Glu Val Lys 
            180                 185                 190         


Lys Met Phe Glu Gly Val Ser Asp Lys Glu Thr Gly Val Thr Val Ser 
        195                 200                 205             


Phe Asn Phe Asn Lys Ala Val Ser Ser Cys Asp Leu Phe Ser Phe Arg 
    210                 215                 220                 


Ser Cys Tyr Glu Leu Glu Ser Glu Trp Leu Asn Leu Val Glu Asp Ile 
225                 230                 235                 240 


Tyr Lys Arg Pro Val Val Pro Val Gly Val Ile Pro Pro Ser Phe Gln 
                245                 250                 255     


Val Arg Ile Val Asn Glu Glu Asp Asn Lys Pro Glu Trp Leu Lys Ile 
            260                 265                 270         


Gln Ser Trp Leu Asp Lys Gln Glu Gln Gly Ser Val Val Tyr Ile Ala 
        275                 280                 285             


Phe Gly Ser Glu Leu Lys Leu Gly Gln Gln Asp Leu Thr Glu Leu Ala 
    290                 295                 300                 


Leu Gly Leu Glu Leu Ser Gly Leu Pro Phe Phe Trp Ala Leu Arg Lys 
305                 310                 315                 320 


Gln Gln Asp Ser Ser Ser Val Asp Leu Pro Asp Gly Phe Glu Asp Arg 
                325                 330                 335     


Val Ser Asp Arg Gly Val Val Cys Arg Asp Trp Val Pro Gln Leu Lys 
            340                 345                 350         


Ile Leu Ala His Gly Ser Ile Gly Gly Tyr Leu Thr His Cys Gly Ser 
        355                 360                 365             


Gly Ser Val Ile Glu Gly Leu His Phe Gly Arg Val Leu Val Met Leu 
    370                 375                 380                 


Pro Tyr Leu Leu Asp Gln Ala Leu Tyr Ala Arg Val Leu Glu Glu Lys 
385                 390                 395                 400 


Lys Leu Gly Val Glu Ile Pro Arg Asn Glu Gln Asp Gly Ser Phe Thr 
                405                 410                 415     


Arg Ser Ser Val Ala Lys Ser Val Lys Leu Ala Ile Val Asp Glu Gly 
            420                 425                 430         


Gly Ser Ile Tyr Arg Asp Lys Ala Lys Glu Met Gly Leu Val Phe Ser 
        435                 440                 445             


Asp Lys Asp Arg His Glu Gln Tyr Ile Glu Asn Phe Leu Gln His Leu 
    450                 455                 460                 


Gln His Lys Arg Glu Pro Phe Gln Ile 
465                 470             


<210>  5
<211>  1395
<212>  DNA
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  A nucleic acid molecule comprising a nucleotide which encodes the
       enzyme according to SEQ ID NO 6.

<400>  5
atggctgctg cagctcccaa tcacaggctc cacatagcat tcttcccatg gttagcattt       60

ggtcacataa accctttctt tgagcttgcg aagctcattg ctcaaaaggg tcatcatatt      120

tctttcattt ccaccccaag aaacatccaa cgcctttcac aagttcctcc acaattagca      180

gattccatag atctagtgag cttaccagta atccataatt caaacctccc agaaaacgca      240

gagtccacca tggacattcc acctgataaa accccttacc ttgggatgct tcacgacagt      300

ctcaaagaac cccttactca attccttcaa actcattccc ctgattggat tctgtatgac      360

ttttcagctg gttggcttgc tgccatagta gaagaccttg gtatctccca cggctacttt      420

tctatcattc cctgttggaa cataggcttc aatggacgcc aaatgaatgg tttccaaaag      480

ccagatattt cattgccctc cgccgtgtcg cttaagaaat atgaggtgaa gaaaatcatg      540

gatttggtca aatcttttcc caaaattttg gatgagtcgg ccactaaatc catagcttcg      600

cattcgacct gtgaagtaat ttttatacga aattgccccg agattgaagc agattggttc      660

gactatactt cgaaaatttt cgataaacca gtggttccgg tgggcgtagt gcccccatct      720

gtgcacataa ctaacaaaga gaaggacgag catttcaaca aatggttgga gatcaaagaa      780

tggttggatc aacaagacag aggttctgta atttatatag cttttggaac tgaatcactg      840

ccaaatcagg atgaaatcac catgcttgct caagggcttg agctatgtgg gcttcctttc      900

ttttgggcat taaggaagtc taatgtggct tctgatcagc caaattcaga ctcagttgag      960

ctaccggagg gatttgaaga acaaaccaaa ggtcgtggaa ttgtgtggac gagttgggca     1020

cctcaacaga gaattctggg tcacaattca attgggggtt ttgtgactca ctgtggttgg     1080

agttcagtaa tagaaggaat tcactatgga cggccactga ttatgttccc tctaacagtc     1140

gaacagtctc tgaatgctag gattttgggg gagaagaagt tgggtatgga agtacccaga     1200

gaagatgatg ggtcttttac aggtgaagtt gtggcagaga cattgaagct ggtattgctg     1260

gaccaagatg ggaaagttta cagggacaag gtaacagaga tgagtaaggt atttggggac     1320

aaagacaaac atgagaaata catgggtgat ctacttgaat tcttcaaaaa ttacaggtct     1380

cttaagagga attag                                                      1395


<210>  6
<211>  464
<212>  PRT
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  A xylosyltransferase enzyme capable of transferring 
       beta-1,4-d-xylopyranose to QA-FR (Qs-28-O-XylT3)

<400>  6

Met Ala Ala Ala Ala Pro Asn His Arg Leu His Ile Ala Phe Phe Pro 
1               5                   10                  15      


Trp Leu Ala Phe Gly His Ile Asn Pro Phe Phe Glu Leu Ala Lys Leu 
            20                  25                  30          


Ile Ala Gln Lys Gly His His Ile Ser Phe Ile Ser Thr Pro Arg Asn 
        35                  40                  45              


Ile Gln Arg Leu Ser Gln Val Pro Pro Gln Leu Ala Asp Ser Ile Asp 
    50                  55                  60                  


Leu Val Ser Leu Pro Val Ile His Asn Ser Asn Leu Pro Glu Asn Ala 
65                  70                  75                  80  


Glu Ser Thr Met Asp Ile Pro Pro Asp Lys Thr Pro Tyr Leu Gly Met 
                85                  90                  95      


Leu His Asp Ser Leu Lys Glu Pro Leu Thr Gln Phe Leu Gln Thr His 
            100                 105                 110         


Ser Pro Asp Trp Ile Leu Tyr Asp Phe Ser Ala Gly Trp Leu Ala Ala 
        115                 120                 125             


Ile Val Glu Asp Leu Gly Ile Ser His Gly Tyr Phe Ser Ile Ile Pro 
    130                 135                 140                 


Cys Trp Asn Ile Gly Phe Asn Gly Arg Gln Met Asn Gly Phe Gln Lys 
145                 150                 155                 160 


Pro Asp Ile Ser Leu Pro Ser Ala Val Ser Leu Lys Lys Tyr Glu Val 
                165                 170                 175     


Lys Lys Ile Met Asp Leu Val Lys Ser Phe Pro Lys Ile Leu Asp Glu 
            180                 185                 190         


Ser Ala Thr Lys Ser Ile Ala Ser His Ser Thr Cys Glu Val Ile Phe 
        195                 200                 205             


Ile Arg Asn Cys Pro Glu Ile Glu Ala Asp Trp Phe Asp Tyr Thr Ser 
    210                 215                 220                 


Lys Ile Phe Asp Lys Pro Val Val Pro Val Gly Val Val Pro Pro Ser 
225                 230                 235                 240 


Val His Ile Thr Asn Lys Glu Lys Asp Glu His Phe Asn Lys Trp Leu 
                245                 250                 255     


Glu Ile Lys Glu Trp Leu Asp Gln Gln Asp Arg Gly Ser Val Ile Tyr 
            260                 265                 270         


Ile Ala Phe Gly Thr Glu Ser Leu Pro Asn Gln Asp Glu Ile Thr Met 
        275                 280                 285             


Leu Ala Gln Gly Leu Glu Leu Cys Gly Leu Pro Phe Phe Trp Ala Leu 
    290                 295                 300                 


Arg Lys Ser Asn Val Ala Ser Asp Gln Pro Asn Ser Asp Ser Val Glu 
305                 310                 315                 320 


Leu Pro Glu Gly Phe Glu Glu Gln Thr Lys Gly Arg Gly Ile Val Trp 
                325                 330                 335     


Thr Ser Trp Ala Pro Gln Gln Arg Ile Leu Gly His Asn Ser Ile Gly 
            340                 345                 350         


Gly Phe Val Thr His Cys Gly Trp Ser Ser Val Ile Glu Gly Ile His 
        355                 360                 365             


Tyr Gly Arg Pro Leu Ile Met Phe Pro Leu Thr Val Glu Gln Ser Leu 
    370                 375                 380                 


Asn Ala Arg Ile Leu Gly Glu Lys Lys Leu Gly Met Glu Val Pro Arg 
385                 390                 395                 400 


Glu Asp Asp Gly Ser Phe Thr Gly Glu Val Val Ala Glu Thr Leu Lys 
                405                 410                 415     


Leu Val Leu Leu Asp Gln Asp Gly Lys Val Tyr Arg Asp Lys Val Thr 
            420                 425                 430         


Glu Met Ser Lys Val Phe Gly Asp Lys Asp Lys His Glu Lys Tyr Met 
        435                 440                 445             


Gly Asp Leu Leu Glu Phe Phe Lys Asn Tyr Arg Ser Leu Lys Arg Asn 
    450                 455                 460                 


<210>  7
<211>  1530
<212>  DNA
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  A nucleic acid molecule comprising a nucleotide which encodes the
       enzyme according to SEQ ID NO 8.

<400>  7
atggactcca cccacttgca gccggccact actcctctga aaattcactt catacctttc       60

atatctccgg gtcacatcat cccactctct gaattggctc gtatctttgc ttcacgcggt      120

gagcatgtga ctatcattac cactcctttc aacgctgacc tacttcagaa atccattgac      180

gaagacagag attccggcga gcacattggc atccacaccg ttgaatggtc agccacagag      240

ctaggccttc ctcatgggat tgaaaatctc agcaacgtca ctgacttgga gaccgccatt      300

aagctacaca gggccctcat gctaatgcag aaacagatgg aagatttcat gacccgaaat      360

ccacccgatt gtataattgc cgacacgttt tacccgtggg cctccgaatt tgctaatcgg      420

atgggtatcc cgagactcat tttctacccg tggagtactt tcgcgctctg tttgatggaa      480

tctattcgat ctcccgactc cccgcaccga agattgagtt cggattcgga tccatttgtg      540

gttccgggtc ttcctcaccc gatcatcttg acccgttctc agcttccgga acacgatcga      600

aaggacatag cggacccggc tgcccaactc atggatcagc ataaagaaac tgagatgaag      660

agctacggaa ttattctcaa caatttcgcg gagatcgaaa cagaatacac agagcattac      720

aagaaaataa cgggtcacaa ggtttggcac attggacctg ccgcagcaat tgttcaccga      780

aatgccaaag agaaggcaga gagggtattc aagagtgatg agcatgacaa taaccttgtc      840

atcaattggc tcaactcgaa ggaaccaaac tcagttgttt atgtttgttt cggcagcgga      900

tgtcaattcc ctgataaaca actctatgag attgcatgcg ggttagagtt atctgggcat      960

caatttgttt gggtggttcg cggaaaagat aaacaaatcg atgttaatga cgatggggag     1020

aagacatggt tgcctaaagg gtttgaggaa agaatgaaaa cagaaaataa aggtttgatt     1080

gtaaggggat gggccccaca ggtgctggtt ttggatcatc catcattggg atgtttcttg     1140

acgcattgcg gctggaattc tacgattgag ggaatcacag caggcgttcc tttgatcacg     1200

tggccagtat tcgccgagca attctataat gagaagctaa tcacgcaggt gcatgggaat     1260

ggggtggtgg ttggttcaga ggagtggatc atgttgttca ccgtcgctaa aagcttggta     1320

agtagagaca aaattgagaa tgctgtgagg aagataatgg acggtggtga tgaggctgta     1380

caaatcagaa ggcgggcccg ggaacttgga gaaaaagctt ggaaagctgc ttcaactggg     1440

gggtcctcct acaataatct aaccgcagca attgaagacc ttaagcggtt gagagaggac     1500

cgttcgaagc tgaaaacaaa aacaatttga                                      1530


<210>  8
<211>  509
<212>  PRT
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  A xylosyltransferase enzyme capable of transferring 
       beta-1,3-d-xylopyranose to QA-FRX (Qs-28-O-XylT4)

<400>  8

Met Asp Ser Thr His Leu Gln Pro Ala Thr Thr Pro Leu Lys Ile His 
1               5                   10                  15      


Phe Ile Pro Phe Ile Ser Pro Gly His Ile Ile Pro Leu Ser Glu Leu 
            20                  25                  30          


Ala Arg Ile Phe Ala Ser Arg Gly Glu His Val Thr Ile Ile Thr Thr 
        35                  40                  45              


Pro Phe Asn Ala Asp Leu Leu Gln Lys Ser Ile Asp Glu Asp Arg Asp 
    50                  55                  60                  


Ser Gly Glu His Ile Gly Ile His Thr Val Glu Trp Ser Ala Thr Glu 
65                  70                  75                  80  


Leu Gly Leu Pro His Gly Ile Glu Asn Leu Ser Asn Val Thr Asp Leu 
                85                  90                  95      


Glu Thr Ala Ile Lys Leu His Arg Ala Leu Met Leu Met Gln Lys Gln 
            100                 105                 110         


Met Glu Asp Phe Met Thr Arg Asn Pro Pro Asp Cys Ile Ile Ala Asp 
        115                 120                 125             


Thr Phe Tyr Pro Trp Ala Ser Glu Phe Ala Asn Arg Met Gly Ile Pro 
    130                 135                 140                 


Arg Leu Ile Phe Tyr Pro Trp Ser Thr Phe Ala Leu Cys Leu Met Glu 
145                 150                 155                 160 


Ser Ile Arg Ser Pro Asp Ser Pro His Arg Arg Leu Ser Ser Asp Ser 
                165                 170                 175     


Asp Pro Phe Val Val Pro Gly Leu Pro His Pro Ile Ile Leu Thr Arg 
            180                 185                 190         


Ser Gln Leu Pro Glu His Asp Arg Lys Asp Ile Ala Asp Pro Ala Ala 
        195                 200                 205             


Gln Leu Met Asp Gln His Lys Glu Thr Glu Met Lys Ser Tyr Gly Ile 
    210                 215                 220                 


Ile Leu Asn Asn Phe Ala Glu Ile Glu Thr Glu Tyr Thr Glu His Tyr 
225                 230                 235                 240 


Lys Lys Ile Thr Gly His Lys Val Trp His Ile Gly Pro Ala Ala Ala 
                245                 250                 255     


Ile Val His Arg Asn Ala Lys Glu Lys Ala Glu Arg Val Phe Lys Ser 
            260                 265                 270         


Asp Glu His Asp Asn Asn Leu Val Ile Asn Trp Leu Asn Ser Lys Glu 
        275                 280                 285             


Pro Asn Ser Val Val Tyr Val Cys Phe Gly Ser Gly Cys Gln Phe Pro 
    290                 295                 300                 


Asp Lys Gln Leu Tyr Glu Ile Ala Cys Gly Leu Glu Leu Ser Gly His 
305                 310                 315                 320 


Gln Phe Val Trp Val Val Arg Gly Lys Asp Lys Gln Ile Asp Val Asn 
                325                 330                 335     


Asp Asp Gly Glu Lys Thr Trp Leu Pro Lys Gly Phe Glu Glu Arg Met 
            340                 345                 350         


Lys Thr Glu Asn Lys Gly Leu Ile Val Arg Gly Trp Ala Pro Gln Val 
        355                 360                 365             


Leu Val Leu Asp His Pro Ser Leu Gly Cys Phe Leu Thr His Cys Gly 
    370                 375                 380                 


Trp Asn Ser Thr Ile Glu Gly Ile Thr Ala Gly Val Pro Leu Ile Thr 
385                 390                 395                 400 


Trp Pro Val Phe Ala Glu Gln Phe Tyr Asn Glu Lys Leu Ile Thr Gln 
                405                 410                 415     


Val His Gly Asn Gly Val Val Val Gly Ser Glu Glu Trp Ile Met Leu 
            420                 425                 430         


Phe Thr Val Ala Lys Ser Leu Val Ser Arg Asp Lys Ile Glu Asn Ala 
        435                 440                 445             


Val Arg Lys Ile Met Asp Gly Gly Asp Glu Ala Val Gln Ile Arg Arg 
    450                 455                 460                 


Arg Ala Arg Glu Leu Gly Glu Lys Ala Trp Lys Ala Ala Ser Thr Gly 
465                 470                 475                 480 


Gly Ser Ser Tyr Asn Asn Leu Thr Ala Ala Ile Glu Asp Leu Lys Arg 
                485                 490                 495     


Leu Arg Glu Asp Arg Ser Lys Leu Lys Thr Lys Thr Ile 
            500                 505                 


<210>  9
<211>  1530
<212>  DNA
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  A nucleic acid molecule comprising a nucleotide which encodes the
       enzyme according to SEQ ID NO 10.

<400>  9
atggactcca cccacttgca gccggccact actcctctga aaattcacgt catacctttc       60

atagctccgg gtcacattat cccactctct gaattggctc gtatctttgc ttcacgcggt      120

gagcatgtga ctatcataac cactcctttc aacgctgacc tacttcagaa atccattgac      180

gaagacagag attccggcga gcacattggc atccacaccg ttgaatggtc agccacagag      240

ctaggccttc ctcatggggt tgaaaatctc agcaacgtca ctgacttgga gaccggcatt      300

aagctacaca gggccctcgt gctaatgcag aaacagatgg aagatttcat gacccgaaat      360

ccacccgatt gtataattgc cgacacgttt tacccgtggg cctccgaatt tgctaatcgg      420

atgggtatcc cgagactcat tttcttcccg gggtgtactt tcgcgctctg tttgatggaa      480

tctattcgat ctcccgactc cccgcaccga agattgagtt cggattcgga cccatttgtg      540

gttccgggtc ttcctcaccc gatcatcttg acccgttctc aacttccgga acacgatcga      600

gaggacatag cggacccggc tgcccaattc atggatcagt gtaaagaagc tgcgatgaag      660

agctacggaa ttattctcaa caatttcgcg gagatcgaaa cagaatacac agagcattac      720

aagaaaataa cgggtcacaa ggtttggcac attggacctg ccgcagcaat tgttcaccga      780

aatgccaaag agaaggcaga ggggcttttc aaaagtgacg agcatgacaa taaccttgtc      840

atcaattggc tcaactcgaa ggaaccaaac tcagttgttt atgtttgttt cggcagcgga      900

tgtcaattcc ctgataaaca actctatgag attgcatgcg ggttagagtt atctgggcat      960

caatttattt gggtggttcg cggcaaagat aaacaaatcg atgttaatga cgatgaggag     1020

aagacatggt tgcctaaagg gtttgaggaa agaatgaaaa cagaaaataa aggtttgatt     1080

gtaaggggat gggccccaca ggtgctggtt ttggatcatc catcgttggg atgtttcttg     1140

acgcattgcg gctggaattc tacgattgag ggaatcacag caggtgttcc tttgatcacg     1200

tggccagtat actccgagca attctataat gagaagctaa tcacgcaggt gcatggtaat     1260

ggggtggggg ttggttcaga ggagtggatc atgctgttca gcgtcgctaa aagtttggta     1320

agtagagaca aaattgagaa tgctgtgagg aagataatgg acggtggtga tgaggcttta     1380

gaaatcagaa ggcgggcccg ggaacttgga gaaaaagcta ggaaagctgc ttcaattggg     1440

gggtcctccg acaataatct aaccgcagca attgaagacc ttaagcggtt gagagaggac     1500

cgttcgaagc tgaaaacaaa aacaatttga                                      1530


<210>  10
<211>  509
<212>  PRT
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  An apiosyltransferase enzyme capable of transferring 
       beta-1,3-d-apiofuranose to QA-FRX (Qs-28-O-ApiT4).

<400>  10

Met Asp Ser Thr His Leu Gln Pro Ala Thr Thr Pro Leu Lys Ile His 
1               5                   10                  15      


Val Ile Pro Phe Ile Ala Pro Gly His Ile Ile Pro Leu Ser Glu Leu 
            20                  25                  30          


Ala Arg Ile Phe Ala Ser Arg Gly Glu His Val Thr Ile Ile Thr Thr 
        35                  40                  45              


Pro Phe Asn Ala Asp Leu Leu Gln Lys Ser Ile Asp Glu Asp Arg Asp 
    50                  55                  60                  


Ser Gly Glu His Ile Gly Ile His Thr Val Glu Trp Ser Ala Thr Glu 
65                  70                  75                  80  


Leu Gly Leu Pro His Gly Val Glu Asn Leu Ser Asn Val Thr Asp Leu 
                85                  90                  95      


Glu Thr Gly Ile Lys Leu His Arg Ala Leu Val Leu Met Gln Lys Gln 
            100                 105                 110         


Met Glu Asp Phe Met Thr Arg Asn Pro Pro Asp Cys Ile Ile Ala Asp 
        115                 120                 125             


Thr Phe Tyr Pro Trp Ala Ser Glu Phe Ala Asn Arg Met Gly Ile Pro 
    130                 135                 140                 


Arg Leu Ile Phe Phe Pro Gly Cys Thr Phe Ala Leu Cys Leu Met Glu 
145                 150                 155                 160 


Ser Ile Arg Ser Pro Asp Ser Pro His Arg Arg Leu Ser Ser Asp Ser 
                165                 170                 175     


Asp Pro Phe Val Val Pro Gly Leu Pro His Pro Ile Ile Leu Thr Arg 
            180                 185                 190         


Ser Gln Leu Pro Glu His Asp Arg Glu Asp Ile Ala Asp Pro Ala Ala 
        195                 200                 205             


Gln Phe Met Asp Gln Cys Lys Glu Ala Ala Met Lys Ser Tyr Gly Ile 
    210                 215                 220                 


Ile Leu Asn Asn Phe Ala Glu Ile Glu Thr Glu Tyr Thr Glu His Tyr 
225                 230                 235                 240 


Lys Lys Ile Thr Gly His Lys Val Trp His Ile Gly Pro Ala Ala Ala 
                245                 250                 255     


Ile Val His Arg Asn Ala Lys Glu Lys Ala Glu Gly Leu Phe Lys Ser 
            260                 265                 270         


Asp Glu His Asp Asn Asn Leu Val Ile Asn Trp Leu Asn Ser Lys Glu 
        275                 280                 285             


Pro Asn Ser Val Val Tyr Val Cys Phe Gly Ser Gly Cys Gln Phe Pro 
    290                 295                 300                 


Asp Lys Gln Leu Tyr Glu Ile Ala Cys Gly Leu Glu Leu Ser Gly His 
305                 310                 315                 320 


Gln Phe Ile Trp Val Val Arg Gly Lys Asp Lys Gln Ile Asp Val Asn 
                325                 330                 335     


Asp Asp Glu Glu Lys Thr Trp Leu Pro Lys Gly Phe Glu Glu Arg Met 
            340                 345                 350         


Lys Thr Glu Asn Lys Gly Leu Ile Val Arg Gly Trp Ala Pro Gln Val 
        355                 360                 365             


Leu Val Leu Asp His Pro Ser Leu Gly Cys Phe Leu Thr His Cys Gly 
    370                 375                 380                 


Trp Asn Ser Thr Ile Glu Gly Ile Thr Ala Gly Val Pro Leu Ile Thr 
385                 390                 395                 400 


Trp Pro Val Tyr Ser Glu Gln Phe Tyr Asn Glu Lys Leu Ile Thr Gln 
                405                 410                 415     


Val His Gly Asn Gly Val Gly Val Gly Ser Glu Glu Trp Ile Met Leu 
            420                 425                 430         


Phe Ser Val Ala Lys Ser Leu Val Ser Arg Asp Lys Ile Glu Asn Ala 
        435                 440                 445             


Val Arg Lys Ile Met Asp Gly Gly Asp Glu Ala Leu Glu Ile Arg Arg 
    450                 455                 460                 


Arg Ala Arg Glu Leu Gly Glu Lys Ala Arg Lys Ala Ala Ser Ile Gly 
465                 470                 475                 480 


Gly Ser Ser Asp Asn Asn Leu Thr Ala Ala Ile Glu Asp Leu Lys Arg 
                485                 490                 495     


Leu Arg Glu Asp Arg Ser Lys Leu Lys Thr Lys Thr Ile 
            500                 505                 


<210>  11
<211>  897
<212>  DNA
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  A nucleic acid molecule comprising a nucleotide which encodes the
       enzyme according to SEQ ID NO 12.

<400>  11
atggcagaag caacgcagag gtatgctgtt gtgacaggat ctaataaggg aattggattt       60

gggatatgca agcagttggc ttctaaggga atcaaagtag tgttaacagc tagagatgag      120

aagagaggtc ttgaagcagt tgagaaattg aaagaaatta gtctggctgg tcatgtggtt      180

tttcatcaac tcgatgtgtc tgatcctgct agtgttacta gccttgaaga tttcatcaaa      240

acccagtttg ggaagctaga tattctggta aacaatgctg ggataacagg aacaactgta      300

gatgctgatg ctttagcagc ttcaggcttc ggtacagggg gtgaacgtaa gcctattgat      360

tggagtaagt tagtgataca gacttatgaa tcagttgaaa aagctttcaa cacaaactat      420

tacggtggca aaagaatgac agaagcactt atacccctcc tccagctatc agactcaccc      480

aggattgtta atgtttcctc tgctatggga cagttagaga atatacctag tggatgggca      540

aaggaagtgc tcacagatgt tgataaccta acagaaggaa aattggatga ggtttcaacc      600

cagtttttga aagatttcaa agagggttca ttggaaacca aaggctggcc tagtcttatg      660

tcttcttata tagtctcaaa agctgtttta aatgcctaca caaggattct tgctaagaaa      720

tacccagctt tctgcatcaa ttgtgtagat cctggctatg tgaagacaga cataaaccat      780

catactggcc aattaagtgt tgatgaaggt gctgaaagtc ctgtaagact ggccttgctg      840

cctaatggtg gtccttctgg cgtgttcttc tccaggacag aagaagcacc attttga         897


<210>  12
<211>  298
<212>  PRT
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  An oxidoreductase enzyme capable of enhancing the activity of a 
       fucosyltransferase (QsFucSyn)

<400>  12

Met Ala Glu Ala Thr Gln Arg Tyr Ala Val Val Thr Gly Ser Asn Lys 
1               5                   10                  15      


Gly Ile Gly Phe Gly Ile Cys Lys Gln Leu Ala Ser Lys Gly Ile Lys 
            20                  25                  30          


Val Val Leu Thr Ala Arg Asp Glu Lys Arg Gly Leu Glu Ala Val Glu 
        35                  40                  45              


Lys Leu Lys Glu Ile Ser Leu Ala Gly His Val Val Phe His Gln Leu 
    50                  55                  60                  


Asp Val Ser Asp Pro Ala Ser Val Thr Ser Leu Glu Asp Phe Ile Lys 
65                  70                  75                  80  


Thr Gln Phe Gly Lys Leu Asp Ile Leu Val Asn Asn Ala Gly Ile Thr 
                85                  90                  95      


Gly Thr Thr Val Asp Ala Asp Ala Leu Ala Ala Ser Gly Phe Gly Thr 
            100                 105                 110         


Gly Gly Glu Arg Lys Pro Ile Asp Trp Ser Lys Leu Val Ile Gln Thr 
        115                 120                 125             


Tyr Glu Ser Val Glu Lys Ala Phe Asn Thr Asn Tyr Tyr Gly Gly Lys 
    130                 135                 140                 


Arg Met Thr Glu Ala Leu Ile Pro Leu Leu Gln Leu Ser Asp Ser Pro 
145                 150                 155                 160 


Arg Ile Val Asn Val Ser Ser Ala Met Gly Gln Leu Glu Asn Ile Pro 
                165                 170                 175     


Ser Gly Trp Ala Lys Glu Val Leu Thr Asp Val Asp Asn Leu Thr Glu 
            180                 185                 190         


Gly Lys Leu Asp Glu Val Ser Thr Gln Phe Leu Lys Asp Phe Lys Glu 
        195                 200                 205             


Gly Ser Leu Glu Thr Lys Gly Trp Pro Ser Leu Met Ser Ser Tyr Ile 
    210                 215                 220                 


Val Ser Lys Ala Val Leu Asn Ala Tyr Thr Arg Ile Leu Ala Lys Lys 
225                 230                 235                 240 


Tyr Pro Ala Phe Cys Ile Asn Cys Val Asp Pro Gly Tyr Val Lys Thr 
                245                 250                 255     


Asp Ile Asn His His Thr Gly Gln Leu Ser Val Asp Glu Gly Ala Glu 
            260                 265                 270         


Ser Pro Val Arg Leu Ala Leu Leu Pro Asn Gly Gly Pro Ser Gly Val 
        275                 280                 285             


Phe Phe Ser Arg Thr Glu Glu Ala Pro Phe 
    290                 295             


<210>  13
<211>  1197
<212>  DNA
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  A nucleic acid molecule comprising a nucleotide which encodes the
       enzyme according to SEQ ID NO 14.

<400>  13
atgccaactt tgtacaaaaa agcaggctta atggcgtcgg cgtcaagggt agatctggat       60

ggtaatcaga taaagccgat gacaatttgc atgatcggtg ccggtgggtt cattgggtcc      120

cacctctgcg agaagattat ggcggagaca ccgcacaagg ttctggcatt agatgtctac      180

aatgacaaga tcaagcactt actggagccg gattctcttc aatggaaaga tcgcatccaa      240

ttccaccgca tcaacattaa gcacgattcg aggctcgaag gtctcatcaa gatggcagat      300

ctgactataa atctggctgc tatttgtact cccgcggatt acaacacccg tcctctggac      360

acaatttata gcaatttcat tgacgctctt cctgtggtaa agtactgttc ggagaataac      420

aagcgtctca ttcatttctc tacgtgtgaa gtgtatggga aaacgattgg gagctttctc      480

ccaaaagaca gccctcttct aaaggatcct gaatattttg ttcttaaaga agatgcctcc      540

ccatgcatat ttggtcctat tgaaaagcag agatggtcct acgcatgtgc aaagcaattg      600

attgagaggc tggtttatgc tgagggtgct gagaatggcc ttgagttcac tattgtgcga      660

cctttcaatt ggattggccc cagaatggat ttcatacctg gcattgatgg tccaagtgaa      720

ggtgttccac gggttctggc atgctttagt aacaatctcc ttcgcggtga gccactcaaa      780

ctcgttgatg gtggccaatc ccagagaact tttgtttata tcaaggatgc aattgaagct      840

gttttgttga tgattgaaaa ccctgccagg gctaatggtc atatttttaa tgtgggtaac      900

cctcacaatg aagttacagt tcggcaactt gctgaaatga tgaccgaggt ctattctaag      960

gtaagtggag aaccgtctct tgaggtgcct accattgatg taagctccaa agaattttat     1020

ggtgaaggat acgatgatag tgacaagaga attcctgaca tgaccataat caacaggcaa     1080

cttggctgga accctaagac atcgctctgg gatcttcttg agtcgaccct cacctatcaa     1140

cataggacat atgcagaagc tattaagaaa tcaattgcga aaccagttgc cagctag        1197


<210>  14
<211>  398
<212>  PRT
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  An enzyme capable of enhancing the activity of an 
       apiosyltransferase (QsAXS1).

<400>  14

Met Pro Thr Leu Tyr Lys Lys Ala Gly Leu Met Ala Ser Ala Ser Arg 
1               5                   10                  15      


Val Asp Leu Asp Gly Asn Gln Ile Lys Pro Met Thr Ile Cys Met Ile 
            20                  25                  30          


Gly Ala Gly Gly Phe Ile Gly Ser His Leu Cys Glu Lys Ile Met Ala 
        35                  40                  45              


Glu Thr Pro His Lys Val Leu Ala Leu Asp Val Tyr Asn Asp Lys Ile 
    50                  55                  60                  


Lys His Leu Leu Glu Pro Asp Ser Leu Gln Trp Lys Asp Arg Ile Gln 
65                  70                  75                  80  


Phe His Arg Ile Asn Ile Lys His Asp Ser Arg Leu Glu Gly Leu Ile 
                85                  90                  95      


Lys Met Ala Asp Leu Thr Ile Asn Leu Ala Ala Ile Cys Thr Pro Ala 
            100                 105                 110         


Asp Tyr Asn Thr Arg Pro Leu Asp Thr Ile Tyr Ser Asn Phe Ile Asp 
        115                 120                 125             


Ala Leu Pro Val Val Lys Tyr Cys Ser Glu Asn Asn Lys Arg Leu Ile 
    130                 135                 140                 


His Phe Ser Thr Cys Glu Val Tyr Gly Lys Thr Ile Gly Ser Phe Leu 
145                 150                 155                 160 


Pro Lys Asp Ser Pro Leu Leu Lys Asp Pro Glu Tyr Phe Val Leu Lys 
                165                 170                 175     


Glu Asp Ala Ser Pro Cys Ile Phe Gly Pro Ile Glu Lys Gln Arg Trp 
            180                 185                 190         


Ser Tyr Ala Cys Ala Lys Gln Leu Ile Glu Arg Leu Val Tyr Ala Glu 
        195                 200                 205             


Gly Ala Glu Asn Gly Leu Glu Phe Thr Ile Val Arg Pro Phe Asn Trp 
    210                 215                 220                 


Ile Gly Pro Arg Met Asp Phe Ile Pro Gly Ile Asp Gly Pro Ser Glu 
225                 230                 235                 240 


Gly Val Pro Arg Val Leu Ala Cys Phe Ser Asn Asn Leu Leu Arg Gly 
                245                 250                 255     


Glu Pro Leu Lys Leu Val Asp Gly Gly Gln Ser Gln Arg Thr Phe Val 
            260                 265                 270         


Tyr Ile Lys Asp Ala Ile Glu Ala Val Leu Leu Met Ile Glu Asn Pro 
        275                 280                 285             


Ala Arg Ala Asn Gly His Ile Phe Asn Val Gly Asn Pro His Asn Glu 
    290                 295                 300                 


Val Thr Val Arg Gln Leu Ala Glu Met Met Thr Glu Val Tyr Ser Lys 
305                 310                 315                 320 


Val Ser Gly Glu Pro Ser Leu Glu Val Pro Thr Ile Asp Val Ser Ser 
                325                 330                 335     


Lys Glu Phe Tyr Gly Glu Gly Tyr Asp Asp Ser Asp Lys Arg Ile Pro 
            340                 345                 350         


Asp Met Thr Ile Ile Asn Arg Gln Leu Gly Trp Asn Pro Lys Thr Ser 
        355                 360                 365             


Leu Trp Asp Leu Leu Glu Ser Thr Leu Thr Tyr Gln His Arg Thr Tyr 
    370                 375                 380                 


Ala Glu Ala Ile Lys Lys Ser Ile Ala Lys Pro Val Ala Ser 
385                 390                 395             


<210>  15
<211>  1275
<212>  DNA
<213>  Avena strigosa


<220>
<221>  misc_feature
<223>  A nucleic acid molecule comprising a nucleotide which encodes the
       enzyme according to SEQ ID NO 16.

<400>  15
atggcgcccg agaaaatgcc cgaggaggac gaggaaatcg tcgccggggt cgtcgcaggg       60

aagatcccct cctacgtgct cgagaccagg ctaggcgact gccgcagggc agccgggatc      120

cgccgcgagg cgctgcgccg gatcaccggc agggagatcg acggccttcc cctcgacggc      180

ttcgactacg actcgattct cggacagtgc tgcgagatgc ccgtcgggta cgtgcagctg      240

ccggtcggcg tcgcggggcc gctcgtcctc gacggccgcc gcatatacgt cccgatggcc      300

accacggagg gctgcctaat cgccagcacc aaccgcggat gcaaggccat tgccgagtcc      360

ggaggcgcat ccagcgtcgt gtaccgcgac gggatgaccc gcgcccccgt agcccgcttc      420

ccctccgcac gacgcgccgc agagctcaag ggcttcctgg agaatccggc caactacgac      480

accctgtccg tggtctttaa cagatcaagc agatttgcaa ggctgcaggg ggtcaagtgc      540

gccatggctg ggaggaactt gtacatgagg ttcacctgca gcaccgggga tgccatgggg      600

atgaacatgg tctccaaggg cgtccaaaat gtgctcgact atctgcagga ggacttccct      660

gacatggacg ttgtcagcat ctcaggcaac ttttgttccg acaagaaatc agctgctgta      720

aactggattg aaggccgtgg aaagtccgtg gtttgtgagg cagtaatcag agaggaagtt      780

gtccacaagg ttctcaagac caacgttcag tcactcgtgg agttgaatgt gatcaagaac      840

cttgctggct cagcagttgc tggtgctctt gggggtttca acgcccacgc aagcaacatc      900

gtaacggcta tcttcattgc cactggtcag gatcctgcac agaatgtgga gagctcacag      960

tgtatcacta tgttggaagc tgtaaatgat ggcagagacc ttcacatctc cgttacaatg     1020

ccatctatcg aggtgggcac agttggtgga ggcacgcagc tggcctcaca gtcggcctgc     1080

ttggacctac tgggcgtcaa aggcgccaac agggaatctc cggggtcgaa cgctaggctg     1140

ctggccacgg tggtggctgg tgccgtccta gctggggagc tgtccctcat ctccgcccaa     1200

gctgccggcc atctggtcca gagccacatg aaatacaaca gatccagcaa ggacatgtcc     1260

aagatcgcct gctga                                                      1275


<210>  16
<211>  424
<212>  PRT
<213>  Avena strigosa


<220>
<221>  misc_feature
<223>  AstHMGR (Avena strigosa truncated HMG-CoA reductase) translated 
       nucleotide sequence

<400>  16

Met Ala Pro Glu Lys Met Pro Glu Glu Asp Glu Glu Ile Val Ala Gly 
1               5                   10                  15      


Val Val Ala Gly Lys Ile Pro Ser Tyr Val Leu Glu Thr Arg Leu Gly 
            20                  25                  30          


Asp Cys Arg Arg Ala Ala Gly Ile Arg Arg Glu Ala Leu Arg Arg Ile 
        35                  40                  45              


Thr Gly Arg Glu Ile Asp Gly Leu Pro Leu Asp Gly Phe Asp Tyr Asp 
    50                  55                  60                  


Ser Ile Leu Gly Gln Cys Cys Glu Met Pro Val Gly Tyr Val Gln Leu 
65                  70                  75                  80  


Pro Val Gly Val Ala Gly Pro Leu Val Leu Asp Gly Arg Arg Ile Tyr 
                85                  90                  95      


Val Pro Met Ala Thr Thr Glu Gly Cys Leu Ile Ala Ser Thr Asn Arg 
            100                 105                 110         


Gly Cys Lys Ala Ile Ala Glu Ser Gly Gly Ala Ser Ser Val Val Tyr 
        115                 120                 125             


Arg Asp Gly Met Thr Arg Ala Pro Val Ala Arg Phe Pro Ser Ala Arg 
    130                 135                 140                 


Arg Ala Ala Glu Leu Lys Gly Phe Leu Glu Asn Pro Ala Asn Tyr Asp 
145                 150                 155                 160 


Thr Leu Ser Val Val Phe Asn Arg Ser Ser Arg Phe Ala Arg Leu Gln 
                165                 170                 175     


Gly Val Lys Cys Ala Met Ala Gly Arg Asn Leu Tyr Met Arg Phe Thr 
            180                 185                 190         


Cys Ser Thr Gly Asp Ala Met Gly Met Asn Met Val Ser Lys Gly Val 
        195                 200                 205             


Gln Asn Val Leu Asp Tyr Leu Gln Glu Asp Phe Pro Asp Met Asp Val 
    210                 215                 220                 


Val Ser Ile Ser Gly Asn Phe Cys Ser Asp Lys Lys Ser Ala Ala Val 
225                 230                 235                 240 


Asn Trp Ile Glu Gly Arg Gly Lys Ser Val Val Cys Glu Ala Val Ile 
                245                 250                 255     


Arg Glu Glu Val Val His Lys Val Leu Lys Thr Asn Val Gln Ser Leu 
            260                 265                 270         


Val Glu Leu Asn Val Ile Lys Asn Leu Ala Gly Ser Ala Val Ala Gly 
        275                 280                 285             


Ala Leu Gly Gly Phe Asn Ala His Ala Ser Asn Ile Val Thr Ala Ile 
    290                 295                 300                 


Phe Ile Ala Thr Gly Gln Asp Pro Ala Gln Asn Val Glu Ser Ser Gln 
305                 310                 315                 320 


Cys Ile Thr Met Leu Glu Ala Val Asn Asp Gly Arg Asp Leu His Ile 
                325                 330                 335     


Ser Val Thr Met Pro Ser Ile Glu Val Gly Thr Val Gly Gly Gly Thr 
            340                 345                 350         


Gln Leu Ala Ser Gln Ser Ala Cys Leu Asp Leu Leu Gly Val Lys Gly 
        355                 360                 365             


Ala Asn Arg Glu Ser Pro Gly Ser Asn Ala Arg Leu Leu Ala Thr Val 
    370                 375                 380                 


Val Ala Gly Ala Val Leu Ala Gly Glu Leu Ser Leu Ile Ser Ala Gln 
385                 390                 395                 400 


Ala Ala Gly His Leu Val Gln Ser His Met Lys Tyr Asn Arg Ser Ser 
                405                 410                 415     


Lys Asp Met Ser Lys Ile Ala Cys 
            420                 


<210>  17
<211>  2277
<212>  DNA
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  A nucleic acid molecule comprising a nucleotide which encodes the
       enzyme according to SEQ ID NO 18.

<400>  17
atgtggaggc tgaagatagc agaaggtggt tccgatccat atctgttcag cacaaacaac       60

ttcgtgggtc gccagacatg ggagttcgaa ccggaggccg gcacacctga ggagcgagca      120

gaggtcgaag ctgcccgcca aaacttttac aacaaccgtt accaggtcaa gccctgtgac      180

gacctccttt ggagatatca gttcctgaga gagaagaatt tcaaacaaac aataccgcct      240

gtcaaggttg aagatggcca agaaattact tatgagatgg ccacaacctc aatgcagagg      300

gcggcccgtc acctatcagc cttgcaggcc agcgatggcc attggccagc tcaaattgct      360

ggccccttgt tcttcatgcc acccttggtc ttttgtgtgt acattactgg gcatcttaat      420

acagtattcc catctgaaca tcgcaaagaa atccttcgtt acatgtacta tcaccagaac      480

gaagatggtg ggtggggact gcacatagag ggtcacagca ccatgttttg cacagcactc      540

aactacattt gtatgcgtat ccttggggaa ggaccagagg ggggtcaaga caatgcttgt      600

gccagagcac gaatgtggat tcttgatcat ggtggtgtaa cacatattcc atcttgggga      660

aagacctggc tttcgatact tggtctattt gagtggtctg gaagcaatcc aatgcctcca      720

gagttttgga tccttccttc atttcttcct atgcatccag caaaaatgtg gtgctattgc      780

cggatggttt acatgcccat gtcttattta tatgggaaaa ggtttgttgg cccaatcacg      840

cctctcattg ttcagttaag agaggaaata cacactcaaa attaccatga aatcaactgg      900

aagtcagtcc gccatctatg tgcaaaggag gatatctact atccccatcc actcatccaa      960

gatttgattt gggacagttt gtacatacta acggagcctc ttctcactcg ctggcccttg     1020

aacaagttgg tgcgggagag ggctctccaa gtaacaatga agcatatcca ctatgaagat     1080

gaaaatagtc gatacataac cattggatgt gtggaaaagg tgttatgtat gcttgcttgt     1140

tgggttgatg atccaaatgg agatgctttc aagaagcacc ttgctcgagt cccagattac     1200

gtatgggtct ctgaagatgg aattactatg cagagttttg gtagtcaaga atgggatgct     1260

ggctttgccg tccaggctct gcttgcttct aatcttaccg aggaacttgg ccctgctctt     1320

gccaaaggac atgacttcat aaagcaatct caggttaagg acaatccttc aggtgacttc     1380

aaaagcatgt atcgtcacat ttctagagga tcatggacct tctctgacca agatcatgga     1440

tggcaagttt ctgattgcac tgcagaaggt ctgaagtgtt gcctgctttt gtcgatgttg     1500

ccaccagaaa ttgttggtga aaaaatggaa ccacaaaggc tatttgattc tgtcaatgtg     1560

ctgctctctc tacagagcaa aaaaggtggt ttagctgcct gggagccagc aggggcgcaa     1620

gattggttgg aattactcaa tcccacagaa ttttttgcgg acattgtcgt tgagcatgaa     1680

tatgttgaat gtactggatc agcaattcag gcattagttt tgttcaagaa gctgtatccg     1740

gggcacagga aaaaagagat tgacagtttc attacaaatg ctgtccggtt ccttgagaat     1800

acacaaacgg cagatggctc ttggtatgga aactggggag tttgcttcac ctatggttgt     1860

tggttcgcac tgggagggct agcagcagct ggcaagactt acaacaactg tcctgcaata     1920

cgcaaagctg ttaatttcct acttacaaca caaagagaag acggtggttg gggagaaagc     1980

tatctttcaa gcccaaaaaa gatatatgta cccctggaag gaagccgatc aaatgtggta     2040

catactgcat gggctatgat gggtctaatt catgctgggc aggctgaaag agactcaact     2100

cctcttcatc gtgcagcaaa gttgatcatc aattatcaac tagaaaatgg cgattggccg     2160

caacaggaaa tcactggagt attcatgaaa aactgcatgt tacattaccc tatgtacaga     2220

aacatctacc caatgtgggc tcttgcagaa taccggaggc gggttccatt gccttaa        2277


<210>  18
<211>  758
<212>  PRT
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  An enzyme involved in making beta-amyrin from 2,3-oxidosqualene 
       (QsbAS)

<400>  18

Met Trp Arg Leu Lys Ile Ala Glu Gly Gly Ser Asp Pro Tyr Leu Phe 
1               5                   10                  15      


Ser Thr Asn Asn Phe Val Gly Arg Gln Thr Trp Glu Phe Glu Pro Glu 
            20                  25                  30          


Ala Gly Thr Pro Glu Glu Arg Ala Glu Val Glu Ala Ala Arg Gln Asn 
        35                  40                  45              


Phe Tyr Asn Asn Arg Tyr Gln Val Lys Pro Cys Asp Asp Leu Leu Trp 
    50                  55                  60                  


Arg Tyr Gln Phe Leu Arg Glu Lys Asn Phe Lys Gln Thr Ile Pro Pro 
65                  70                  75                  80  


Val Lys Val Glu Asp Gly Gln Glu Ile Thr Tyr Glu Met Ala Thr Thr 
                85                  90                  95      


Ser Met Gln Arg Ala Ala Arg His Leu Ser Ala Leu Gln Ala Ser Asp 
            100                 105                 110         


Gly His Trp Pro Ala Gln Ile Ala Gly Pro Leu Phe Phe Met Pro Pro 
        115                 120                 125             


Leu Val Phe Cys Val Tyr Ile Thr Gly His Leu Asn Thr Val Phe Pro 
    130                 135                 140                 


Ser Glu His Arg Lys Glu Ile Leu Arg Tyr Met Tyr Tyr His Gln Asn 
145                 150                 155                 160 


Glu Asp Gly Gly Trp Gly Leu His Ile Glu Gly His Ser Thr Met Phe 
                165                 170                 175     


Cys Thr Ala Leu Asn Tyr Ile Cys Met Arg Ile Leu Gly Glu Gly Pro 
            180                 185                 190         


Glu Gly Gly Gln Asp Asn Ala Cys Ala Arg Ala Arg Met Trp Ile Leu 
        195                 200                 205             


Asp His Gly Gly Val Thr His Ile Pro Ser Trp Gly Lys Thr Trp Leu 
    210                 215                 220                 


Ser Ile Leu Gly Leu Phe Glu Trp Ser Gly Ser Asn Pro Met Pro Pro 
225                 230                 235                 240 


Glu Phe Trp Ile Leu Pro Ser Phe Leu Pro Met His Pro Ala Lys Met 
                245                 250                 255     


Trp Cys Tyr Cys Arg Met Val Tyr Met Pro Met Ser Tyr Leu Tyr Gly 
            260                 265                 270         


Lys Arg Phe Val Gly Pro Ile Thr Pro Leu Ile Val Gln Leu Arg Glu 
        275                 280                 285             


Glu Ile His Thr Gln Asn Tyr His Glu Ile Asn Trp Lys Ser Val Arg 
    290                 295                 300                 


His Leu Cys Ala Lys Glu Asp Ile Tyr Tyr Pro His Pro Leu Ile Gln 
305                 310                 315                 320 


Asp Leu Ile Trp Asp Ser Leu Tyr Ile Leu Thr Glu Pro Leu Leu Thr 
                325                 330                 335     


Arg Trp Pro Leu Asn Lys Leu Val Arg Glu Arg Ala Leu Gln Val Thr 
            340                 345                 350         


Met Lys His Ile His Tyr Glu Asp Glu Asn Ser Arg Tyr Ile Thr Ile 
        355                 360                 365             


Gly Cys Val Glu Lys Val Leu Cys Met Leu Ala Cys Trp Val Asp Asp 
    370                 375                 380                 


Pro Asn Gly Asp Ala Phe Lys Lys His Leu Ala Arg Val Pro Asp Tyr 
385                 390                 395                 400 


Val Trp Val Ser Glu Asp Gly Ile Thr Met Gln Ser Phe Gly Ser Gln 
                405                 410                 415     


Glu Trp Asp Ala Gly Phe Ala Val Gln Ala Leu Leu Ala Ser Asn Leu 
            420                 425                 430         


Thr Glu Glu Leu Gly Pro Ala Leu Ala Lys Gly His Asp Phe Ile Lys 
        435                 440                 445             


Gln Ser Gln Val Lys Asp Asn Pro Ser Gly Asp Phe Lys Ser Met Tyr 
    450                 455                 460                 


Arg His Ile Ser Arg Gly Ser Trp Thr Phe Ser Asp Gln Asp His Gly 
465                 470                 475                 480 


Trp Gln Val Ser Asp Cys Thr Ala Glu Gly Leu Lys Cys Cys Leu Leu 
                485                 490                 495     


Leu Ser Met Leu Pro Pro Glu Ile Val Gly Glu Lys Met Glu Pro Gln 
            500                 505                 510         


Arg Leu Phe Asp Ser Val Asn Val Leu Leu Ser Leu Gln Ser Lys Lys 
        515                 520                 525             


Gly Gly Leu Ala Ala Trp Glu Pro Ala Gly Ala Gln Asp Trp Leu Glu 
    530                 535                 540                 


Leu Leu Asn Pro Thr Glu Phe Phe Ala Asp Ile Val Val Glu His Glu 
545                 550                 555                 560 


Tyr Val Glu Cys Thr Gly Ser Ala Ile Gln Ala Leu Val Leu Phe Lys 
                565                 570                 575     


Lys Leu Tyr Pro Gly His Arg Lys Lys Glu Ile Asp Ser Phe Ile Thr 
            580                 585                 590         


Asn Ala Val Arg Phe Leu Glu Asn Thr Gln Thr Ala Asp Gly Ser Trp 
        595                 600                 605             


Tyr Gly Asn Trp Gly Val Cys Phe Thr Tyr Gly Cys Trp Phe Ala Leu 
    610                 615                 620                 


Gly Gly Leu Ala Ala Ala Gly Lys Thr Tyr Asn Asn Cys Pro Ala Ile 
625                 630                 635                 640 


Arg Lys Ala Val Asn Phe Leu Leu Thr Thr Gln Arg Glu Asp Gly Gly 
                645                 650                 655     


Trp Gly Glu Ser Tyr Leu Ser Ser Pro Lys Lys Ile Tyr Val Pro Leu 
            660                 665                 670         


Glu Gly Ser Arg Ser Asn Val Val His Thr Ala Trp Ala Met Met Gly 
        675                 680                 685             


Leu Ile His Ala Gly Gln Ala Glu Arg Asp Ser Thr Pro Leu His Arg 
    690                 695                 700                 


Ala Ala Lys Leu Ile Ile Asn Tyr Gln Leu Glu Asn Gly Asp Trp Pro 
705                 710                 715                 720 


Gln Gln Glu Ile Thr Gly Val Phe Met Lys Asn Cys Met Leu His Tyr 
                725                 730                 735     


Pro Met Tyr Arg Asn Ile Tyr Pro Met Trp Ala Leu Ala Glu Tyr Arg 
            740                 745                 750         


Arg Arg Val Pro Leu Pro 
        755             


<210>  19
<211>  1443
<212>  DNA
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  A nucleic acid molecule comprising a nucleotide which encodes the
       enzyme according to SEQ ID NO 20

<400>  19
atggagcact tgtatctctc ccttgtgctc ctgtttgttt cctcaatctc cctctccctc       60

ttcttcctgt tctacaaaca caaatctatg ttcaccgggg ccaacctacc acctggtaaa      120

atcggttacc cattgatcgg agagagcttg gagttcttgt ccacgggatg gaagggccac      180

ccggagaaat tcatcttcga tcgcatgagc aagtactcat cccaaatctt caagacctcg      240

attttagggg aaccaacggc ggtgttcccg ggagccgtat gcaacaagtt cctcttctcc      300

aacgagaaca agctggtgaa tgcatggtgg cctgcctccg tggacaagat ctttccttcc      360

tcactccaga catcctccaa agaagaggcc aagaagatga ggaagttgct tcctcagttt      420

ctcaagcccg aagctctgca ccgctacatt ggtattatgg attctattgc ccagagacac      480

tttgccgata gctgggaaaa caaaaaccaa gtcattgtct ttcctctagc aaagaggtat      540

actttctggc tggcttgccg tttgttcatt agcgtcgagg atccgaccca cgtatccaga      600

tttgctgacc cgttccaact tttggccgcc ggaatcatat caatcccaat cgacttgcca      660

gggacaccgt tccgcaaggc aatcaatgcg tcccagttca tcaggaagga attgttggcc      720

atcatcaggc agagaaagat cgatttgggt gaagggaagg catctccgac gcaggacata      780

ctgtctcaca tgttgctcac atgcgacgag aacggacaat acatgaatga attggacatt      840

gccgacaaga ttcttggctt gttggtcggc ggacatgaca ctgccagtgc cgcttgcact      900

ttcattgtca agttcctcgc tgagcttccc cacatttatg aacaagtcta caaggagcaa      960

atggagattg caaaatcaaa agtgccagga gagttgttga attgggagga catccaaaag     1020

atgaaatatt cgtggaacgt agcttgtgaa gtgatgagac ttgcccctcc actccaagga     1080

gctttcaggg aagccattac tgacttcgtc ttcaacggtt tctccattcc aaaaggctgg     1140

aagttgtact ggagcgcaaa ttccacccac aaaagtccgg attatttccc tgagcccgac     1200

aagttcgacc caactagatt cgaaggaaat ggacctgcgc cttacacctt tgttccattt     1260

gggggaggac ccaggatgtg cccgggcaaa gagtatgccc gattggaaat acttgtgttc     1320

atgcataact tggtgaagag gttcaagtgg gagaaattgg ttcctgatga aaagattgtg     1380

gttgatccaa tgcccattcc agcaaagggt cttcctgttc gcctttatcc tcacaaagct     1440

tga                                                                   1443


<210>  20
<211>  480
<212>  PRT
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  An enzyme involved in making Oleanolic acid from beta-amyrin 
       (QsCYP716-C-28)

<400>  20

Met Glu His Leu Tyr Leu Ser Leu Val Leu Leu Phe Val Ser Ser Ile 
1               5                   10                  15      


Ser Leu Ser Leu Phe Phe Leu Phe Tyr Lys His Lys Ser Met Phe Thr 
            20                  25                  30          


Gly Ala Asn Leu Pro Pro Gly Lys Ile Gly Tyr Pro Leu Ile Gly Glu 
        35                  40                  45              


Ser Leu Glu Phe Leu Ser Thr Gly Trp Lys Gly His Pro Glu Lys Phe 
    50                  55                  60                  


Ile Phe Asp Arg Met Ser Lys Tyr Ser Ser Gln Ile Phe Lys Thr Ser 
65                  70                  75                  80  


Ile Leu Gly Glu Pro Thr Ala Val Phe Pro Gly Ala Val Cys Asn Lys 
                85                  90                  95      


Phe Leu Phe Ser Asn Glu Asn Lys Leu Val Asn Ala Trp Trp Pro Ala 
            100                 105                 110         


Ser Val Asp Lys Ile Phe Pro Ser Ser Leu Gln Thr Ser Ser Lys Glu 
        115                 120                 125             


Glu Ala Lys Lys Met Arg Lys Leu Leu Pro Gln Phe Leu Lys Pro Glu 
    130                 135                 140                 


Ala Leu His Arg Tyr Ile Gly Ile Met Asp Ser Ile Ala Gln Arg His 
145                 150                 155                 160 


Phe Ala Asp Ser Trp Glu Asn Lys Asn Gln Val Ile Val Phe Pro Leu 
                165                 170                 175     


Ala Lys Arg Tyr Thr Phe Trp Leu Ala Cys Arg Leu Phe Ile Ser Val 
            180                 185                 190         


Glu Asp Pro Thr His Val Ser Arg Phe Ala Asp Pro Phe Gln Leu Leu 
        195                 200                 205             


Ala Ala Gly Ile Ile Ser Ile Pro Ile Asp Leu Pro Gly Thr Pro Phe 
    210                 215                 220                 


Arg Lys Ala Ile Asn Ala Ser Gln Phe Ile Arg Lys Glu Leu Leu Ala 
225                 230                 235                 240 


Ile Ile Arg Gln Arg Lys Ile Asp Leu Gly Glu Gly Lys Ala Ser Pro 
                245                 250                 255     


Thr Gln Asp Ile Leu Ser His Met Leu Leu Thr Cys Asp Glu Asn Gly 
            260                 265                 270         


Gln Tyr Met Asn Glu Leu Asp Ile Ala Asp Lys Ile Leu Gly Leu Leu 
        275                 280                 285             


Val Gly Gly His Asp Thr Ala Ser Ala Ala Cys Thr Phe Ile Val Lys 
    290                 295                 300                 


Phe Leu Ala Glu Leu Pro His Ile Tyr Glu Gln Val Tyr Lys Glu Gln 
305                 310                 315                 320 


Met Glu Ile Ala Lys Ser Lys Val Pro Gly Glu Leu Leu Asn Trp Glu 
                325                 330                 335     


Asp Ile Gln Lys Met Lys Tyr Ser Trp Asn Val Ala Cys Glu Val Met 
            340                 345                 350         


Arg Leu Ala Pro Pro Leu Gln Gly Ala Phe Arg Glu Ala Ile Thr Asp 
        355                 360                 365             


Phe Val Phe Asn Gly Phe Ser Ile Pro Lys Gly Trp Lys Leu Tyr Trp 
    370                 375                 380                 


Ser Ala Asn Ser Thr His Lys Ser Pro Asp Tyr Phe Pro Glu Pro Asp 
385                 390                 395                 400 


Lys Phe Asp Pro Thr Arg Phe Glu Gly Asn Gly Pro Ala Pro Tyr Thr 
                405                 410                 415     


Phe Val Pro Phe Gly Gly Gly Pro Arg Met Cys Pro Gly Lys Glu Tyr 
            420                 425                 430         


Ala Arg Leu Glu Ile Leu Val Phe Met His Asn Leu Val Lys Arg Phe 
        435                 440                 445             


Lys Trp Glu Lys Leu Val Pro Asp Glu Lys Ile Val Val Asp Pro Met 
    450                 455                 460                 


Pro Ile Pro Ala Lys Gly Leu Pro Val Arg Leu Tyr Pro His Lys Ala 
465                 470                 475                 480 


<210>  21
<211>  1506
<212>  DNA
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  A nucleic acid molecule comprising a nucleotide which encodes the
       enzyme according to SEQ ID NO 22.

<400>  21
atgatatata ataatgatag taatgataat gaattagtaa tcagctcagt tcagcaacca       60

tccatggatc ctttcttcat ttttggctta cttctcttgg ctctctttct ctctgtttct      120

tttcttctct acctttcccg tagagcctat gcttctctcc ccaaccctcc gccggggaag      180

ctcggcttcc ccgtcgtcgg cgagagtctc gaatttctct ccacccgacg caaaggtgtt      240

cctgagaaat tcgtcttcga cagaatggcc aaatactgtc gggatgtctt taagacatca      300

atattgggag caaccaccgc cgtcatgtgc ggcaccgccg gtaacaaatt cttgttctcc      360

aacgagaaaa aacacgtcac tggttggtgg ccgaaatctg tagagctgat tttcccaacc      420

tcacttgaga aatcatccaa cgaagaatcc atcatgatga aacaattcct tcccaacttc      480

ttgaaaccag aacctttgca gaagtacata cccgttatgg acataattac ccaaagacac      540

ttcaatacaa gctgggaagg acgcaacgtg gtcaaagtgt ttcctacggc tgccgaattc      600

accacgttgc tggcttgtcg ggtattcctc agtgttgagg atcccattga agtagccaag      660

atttcagagc catttgaaat cttagctgct gggtttcttt caatacccat aaatcttccg      720

ggtaccaaat taaataaagc ggttaaggca gcggatcaga ttagagacgc aattgtacag      780

attttgaaac ggagaagggt tgaaattgcg gagaataaag caaatggaat gcaagatata      840

gcgtccatgt tgttgacgac accaactaat gctgggtttt atatgaccga ggctcacatt      900

tctgagaaaa ttttgggtat gattgttggt ggccgtgata ctgctagtac tgttatcacc      960

ttcatcatca agtatttggc agagaatcct gaaatttata ataaggtcta tgaggagcaa     1020

atggaagtgg taaagtcaaa gaaaccaggt gagttgctga actgggaaga tgtgcagaaa     1080

atgaagtact cttggtgcgt agcatgtgaa gctatgcgac ttgctcctcc tgttcaaggt     1140

ggtttcaagg tggccattaa tgactttgtg tattctgggt tcaacattcg caagggttgg     1200

aagttatatt ggagtgccat tgcaacacac atgaatccag aatatttccc agaacctgag     1260

aaattcaacc cctcaaggtt tgaagggaag ggaccagtac cttacagctt cgtacccttc     1320

ggaggcggac ctcggatgtg tcccgggaaa gagtattccc ggctggaaac acttgttttc     1380

atgcatcatt tggtgacgag gtacaattgg gagaaagtgt atcccacaga gaagataaca     1440

gtggatccaa tgccattccc tgtcaacggc ctccccattc gccttattcc tcacaagcac     1500

caatga                                                                1506


<210>  22
<211>  501
<212>  PRT
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  An enzyme involved in making Echinocystic acid from Oleanolic 
       acid (QsCYP716-C-16alpha)

<400>  22

Met Ile Tyr Asn Asn Asp Ser Asn Asp Asn Glu Leu Val Ile Ser Ser 
1               5                   10                  15      


Val Gln Gln Pro Ser Met Asp Pro Phe Phe Ile Phe Gly Leu Leu Leu 
            20                  25                  30          


Leu Ala Leu Phe Leu Ser Val Ser Phe Leu Leu Tyr Leu Ser Arg Arg 
        35                  40                  45              


Ala Tyr Ala Ser Leu Pro Asn Pro Pro Pro Gly Lys Leu Gly Phe Pro 
    50                  55                  60                  


Val Val Gly Glu Ser Leu Glu Phe Leu Ser Thr Arg Arg Lys Gly Val 
65                  70                  75                  80  


Pro Glu Lys Phe Val Phe Asp Arg Met Ala Lys Tyr Cys Arg Asp Val 
                85                  90                  95      


Phe Lys Thr Ser Ile Leu Gly Ala Thr Thr Ala Val Met Cys Gly Thr 
            100                 105                 110         


Ala Gly Asn Lys Phe Leu Phe Ser Asn Glu Lys Lys His Val Thr Gly 
        115                 120                 125             


Trp Trp Pro Lys Ser Val Glu Leu Ile Phe Pro Thr Ser Leu Glu Lys 
    130                 135                 140                 


Ser Ser Asn Glu Glu Ser Ile Met Met Lys Gln Phe Leu Pro Asn Phe 
145                 150                 155                 160 


Leu Lys Pro Glu Pro Leu Gln Lys Tyr Ile Pro Val Met Asp Ile Ile 
                165                 170                 175     


Thr Gln Arg His Phe Asn Thr Ser Trp Glu Gly Arg Asn Val Val Lys 
            180                 185                 190         


Val Phe Pro Thr Ala Ala Glu Phe Thr Thr Leu Leu Ala Cys Arg Val 
        195                 200                 205             


Phe Leu Ser Val Glu Asp Pro Ile Glu Val Ala Lys Ile Ser Glu Pro 
    210                 215                 220                 


Phe Glu Ile Leu Ala Ala Gly Phe Leu Ser Ile Pro Ile Asn Leu Pro 
225                 230                 235                 240 


Gly Thr Lys Leu Asn Lys Ala Val Lys Ala Ala Asp Gln Ile Arg Asp 
                245                 250                 255     


Ala Ile Val Gln Ile Leu Lys Arg Arg Arg Val Glu Ile Ala Glu Asn 
            260                 265                 270         


Lys Ala Asn Gly Met Gln Asp Ile Ala Ser Met Leu Leu Thr Thr Pro 
        275                 280                 285             


Thr Asn Ala Gly Phe Tyr Met Thr Glu Ala His Ile Ser Glu Lys Ile 
    290                 295                 300                 


Leu Gly Met Ile Val Gly Gly Arg Asp Thr Ala Ser Thr Val Ile Thr 
305                 310                 315                 320 


Phe Ile Ile Lys Tyr Leu Ala Glu Asn Pro Glu Ile Tyr Asn Lys Val 
                325                 330                 335     


Tyr Glu Glu Gln Met Glu Val Val Lys Ser Lys Lys Pro Gly Glu Leu 
            340                 345                 350         


Leu Asn Trp Glu Asp Val Gln Lys Met Lys Tyr Ser Trp Cys Val Ala 
        355                 360                 365             


Cys Glu Ala Met Arg Leu Ala Pro Pro Val Gln Gly Gly Phe Lys Val 
    370                 375                 380                 


Ala Ile Asn Asp Phe Val Tyr Ser Gly Phe Asn Ile Arg Lys Gly Trp 
385                 390                 395                 400 


Lys Leu Tyr Trp Ser Ala Ile Ala Thr His Met Asn Pro Glu Tyr Phe 
                405                 410                 415     


Pro Glu Pro Glu Lys Phe Asn Pro Ser Arg Phe Glu Gly Lys Gly Pro 
            420                 425                 430         


Val Pro Tyr Ser Phe Val Pro Phe Gly Gly Gly Pro Arg Met Cys Pro 
        435                 440                 445             


Gly Lys Glu Tyr Ser Arg Leu Glu Thr Leu Val Phe Met His His Leu 
    450                 455                 460                 


Val Thr Arg Tyr Asn Trp Glu Lys Val Tyr Pro Thr Glu Lys Ile Thr 
465                 470                 475                 480 


Val Asp Pro Met Pro Phe Pro Val Asn Gly Leu Pro Ile Arg Leu Ile 
                485                 490                 495     


Pro His Lys His Gln 
            500     


<210>  23
<211>  1524
<212>  DNA
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  A nucleic acid molecule comprising a nucleotide which encodes the
       enzyme according to SEQ ID NO 24.

<400>  23
atgtggttca cagtaggatt ggtcttggtt ttcgccctat tcatacgtct ctacagcagt       60

ctgtggttga agcctcgtgc aactcggatt aagcttagca atcaaggaat taaaggtcca      120

aaaccagcat ttcttctggg taatgttgca gagatgagaa gatttcaatc taagcttcca      180

aaatctgaac tcaaacaagg ccaagtttct catgattggg cttctaaatc tctgtttcca      240

tttttcagtc tttggtccca gaaatacgga aatacgttcg tgttctcatt ggggaacata      300

caggtgctct atgtttctga tcatgagttg gtgaaagaaa ttaatcagaa tacctcttta      360

gatttgggca aacccaagta cctgcagaag gagcgtggcc ctttgctggg acaaggtatt      420

ttgacctcca atggacagct ttgggcgtac cagagaaaaa tcatgactcc tgaactctac      480

aaggagaaaa tcaagggcat gtgcgagttg atggtggaat ctgtagcttg gttggttgag      540

gaatggggaa cgaagatcca agctgagggt ggggcagcag acattagaat agacgaggat      600

cttagaagct tctctggtga tgtaatttca aaagcttgtt ttgggagctg ctatgccgga      660

gggagggaaa tctttcttag gctcagagct cttcaacacc aaattgcttc caaagcctta      720

ctcatgggct tccctggatt aaagtacctg cccattaaga gcaacagaga gatatggaga      780

ttggagaagg agatcttcca gctgattatg aagctggctg aagatagaaa aaaagaacaa      840

catgagagag acctattaca gattataatt gagggagcta aaagtagtga tctgagttcg      900

gaagcaatgg caaaattcat tgtggacaac tgcaagaatg tctacttggc tggccatgaa      960

actactgcaa tgtctgctgg ttggactttg cttctcttgg ctaatcatcc tgagtggcaa     1020

gcccgtgtcc gtgatgagat tttacaagtc accgagggcc gcaatcctga ttttgacatg     1080

ctgcacaaga tgaaactgtt aacaatggta attcaggagg cactgcgact ctacccaaca     1140

gtcatattca tgtcaagaga agcattggaa gatattaatg ttggaaacat ccaagttcca     1200

aaaggtgtta acatatggat acctgtggta aatcttcaaa gggacacaac ggtatggggt     1260

gcagacgcaa acgagtttaa tcctgaaagg tttgccaatg gagttaacaa ttcatgcaag     1320

gttccacaac tttacctacc atttggagct ggacctcgca tttgtcctgg aattaatctg     1380

gccatgactg agatcaagat acttctgtgt atcctgctca ccaagttttc gttttcagtt     1440

tcacccaact atcgccactc accggtgttt aaattggtgc ttgagcctga aaatggaatc     1500

aatgtcatca tgaagaagct ctaa                                            1524


<210>  24
<211>  507
<212>  PRT
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  An enzyme involved in making Quillaic acid from Echinocystic acid
       (QsCYP714-C-23).

<400>  24

Met Trp Phe Thr Val Gly Leu Val Leu Val Phe Ala Leu Phe Ile Arg 
1               5                   10                  15      


Leu Tyr Ser Ser Leu Trp Leu Lys Pro Arg Ala Thr Arg Ile Lys Leu 
            20                  25                  30          


Ser Asn Gln Gly Ile Lys Gly Pro Lys Pro Ala Phe Leu Leu Gly Asn 
        35                  40                  45              


Val Ala Glu Met Arg Arg Phe Gln Ser Lys Leu Pro Lys Ser Glu Leu 
    50                  55                  60                  


Lys Gln Gly Gln Val Ser His Asp Trp Ala Ser Lys Ser Leu Phe Pro 
65                  70                  75                  80  


Phe Phe Ser Leu Trp Ser Gln Lys Tyr Gly Asn Thr Phe Val Phe Ser 
                85                  90                  95      


Leu Gly Asn Ile Gln Val Leu Tyr Val Ser Asp His Glu Leu Val Lys 
            100                 105                 110         


Glu Ile Asn Gln Asn Thr Ser Leu Asp Leu Gly Lys Pro Lys Tyr Leu 
        115                 120                 125             


Gln Lys Glu Arg Gly Pro Leu Leu Gly Gln Gly Ile Leu Thr Ser Asn 
    130                 135                 140                 


Gly Gln Leu Trp Ala Tyr Gln Arg Lys Ile Met Thr Pro Glu Leu Tyr 
145                 150                 155                 160 


Lys Glu Lys Ile Lys Gly Met Cys Glu Leu Met Val Glu Ser Val Ala 
                165                 170                 175     


Trp Leu Val Glu Glu Trp Gly Thr Lys Ile Gln Ala Glu Gly Gly Ala 
            180                 185                 190         


Ala Asp Ile Arg Ile Asp Glu Asp Leu Arg Ser Phe Ser Gly Asp Val 
        195                 200                 205             


Ile Ser Lys Ala Cys Phe Gly Ser Cys Tyr Ala Gly Gly Arg Glu Ile 
    210                 215                 220                 


Phe Leu Arg Leu Arg Ala Leu Gln His Gln Ile Ala Ser Lys Ala Leu 
225                 230                 235                 240 


Leu Met Gly Phe Pro Gly Leu Lys Tyr Leu Pro Ile Lys Ser Asn Arg 
                245                 250                 255     


Glu Ile Trp Arg Leu Glu Lys Glu Ile Phe Gln Leu Ile Met Lys Leu 
            260                 265                 270         


Ala Glu Asp Arg Lys Lys Glu Gln His Glu Arg Asp Leu Leu Gln Ile 
        275                 280                 285             


Ile Ile Glu Gly Ala Lys Ser Ser Asp Leu Ser Ser Glu Ala Met Ala 
    290                 295                 300                 


Lys Phe Ile Val Asp Asn Cys Lys Asn Val Tyr Leu Ala Gly His Glu 
305                 310                 315                 320 


Thr Thr Ala Met Ser Ala Gly Trp Thr Leu Leu Leu Leu Ala Asn His 
                325                 330                 335     


Pro Glu Trp Gln Ala Arg Val Arg Asp Glu Ile Leu Gln Val Thr Glu 
            340                 345                 350         


Gly Arg Asn Pro Asp Phe Asp Met Leu His Lys Met Lys Leu Leu Thr 
        355                 360                 365             


Met Val Ile Gln Glu Ala Leu Arg Leu Tyr Pro Thr Val Ile Phe Met 
    370                 375                 380                 


Ser Arg Glu Ala Leu Glu Asp Ile Asn Val Gly Asn Ile Gln Val Pro 
385                 390                 395                 400 


Lys Gly Val Asn Ile Trp Ile Pro Val Val Asn Leu Gln Arg Asp Thr 
                405                 410                 415     


Thr Val Trp Gly Ala Asp Ala Asn Glu Phe Asn Pro Glu Arg Phe Ala 
            420                 425                 430         


Asn Gly Val Asn Asn Ser Cys Lys Val Pro Gln Leu Tyr Leu Pro Phe 
        435                 440                 445             


Gly Ala Gly Pro Arg Ile Cys Pro Gly Ile Asn Leu Ala Met Thr Glu 
    450                 455                 460                 


Ile Lys Ile Leu Leu Cys Ile Leu Leu Thr Lys Phe Ser Phe Ser Val 
465                 470                 475                 480 


Ser Pro Asn Tyr Arg His Ser Pro Val Phe Lys Leu Val Leu Glu Pro 
                485                 490                 495     


Glu Asn Gly Ile Asn Val Ile Met Lys Lys Leu 
            500                 505         


<210>  25
<211>  2142
<212>  DNA
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  A nucleic acid molecule comprising a nucleotide which encodes the
       enzyme according to SEQ ID NO 26.

<400>  25
atgaaatccc cctctaaccc aaatcagaaa cccatcctcc acacttgtac aattcagcag       60

cctcgtgcta cccttaacaa aattcatagt cttattcatt tctcagccat acttgtccta      120

ttttattacc ggataacccg tctattcttc accgacgatt tcaaggtacc caagttacta      180

tggactctaa tgacaatctc cgagttcatt cttgccttca tttgggttct catccaacct      240

ttccggtggc gaccggtgtc ccgttccgtc ataccagaga atatgccgaa ggacatcagt      300

ttgccggcgg tggacgtgtt tgtatgcaca gctgaccctc aaaaagaacc cacagtggag      360

gtgatgaaca caattttatc agccatggct ttagactacc cggcggagaa gctcgccgtg      420

tatctttccg atgatggggg ttctgctgtc accttatatg ctataaaaga agcttgttgt      480

tttgctaaga tgtggcttcc gttttgtaac aagtatggga tcaaatcaag gtgtcccgag      540

gcttattttt caaagcttgc cgctgacgag tggcttcacc ggagtgtgga attcgtggca      600

gaagaaaagg aggtcaaggc taattatgaa gagttcaaga gaaatgtgca gaaatttggt      660

gagcaacaag aaaacagtcg tgttgtgcat gatcgtcacc ctcatgttga gattatacac      720

aataattgga ataacgaaga ccaagctcat gagatgccac tccttgttta tgtctctcgt      780

gaaagaagac catctcacca tcctcgattc aaagctggag ctcttaacac ccttcttcga      840

gtttctggca tcatcagcaa cagcccctac atactggttc tagactgtga catgtactgc      900

aatgacccaa cctcagctag acaagcaatg tgcttccatc ttgatcccca actgtctaaa      960

aatcttgctt ttgtacaatt ccctcaaata ttctataacg ctagtaagaa tgacgtctat     1020

gatgcccaag tcagggcggc ataccagaca aagtggcagg gtatggatgg acttcaagga     1080

ccaatttttt ctggcactgg cttttactta aagaggaagg caatgtatgg aaaccctgat     1140

caagatgata attgtctact caagccatat aagaaatttg gcatgtctgg agaatttgta     1200

gaatcactta aggtccttaa cgaacaagat ggtacccaga agaaattatt ggatggattt     1260

ttacaagagg ccaaactatt ggcctcgtgt gcctatgaaa caaagacaag ttggggtaaa     1320

gagattggat tctcatatga ctgtttaata gagagcactt tcactggtta tcttttgcac     1380

tgcagagggt ggatatctgt ttatctttat cccaagagac catgtttttt aggatgctgt     1440

cctactgata tgaaggatgc catggttcaa tataccaagt ggatgtctga gctattttca     1500

attgctatct caagattcaa tcctctgctc tatggggtgg caagaatgtc cattcttcaa     1560

agcctgtgtt atggatcctt tacactggcg cctattttgt catttccttt gttcttatat     1620

ggaacggttc ctcaattatg cctcttgaaa ggcatatctt tgtttccaaa ggtttcggac     1680

ccatggtttg ctgtgtttgc agctatcttt gtatcctccc tgtgtcaaca ctggttcgag     1740

gtcctctctt gtgatggtac atttacgact tggtgtaatg aacagcggag ttggcttata     1800

aagtcggttt ccggtagttt gtttggagtt gtgggcgcaa tcttgcagcg gctaggcttg     1860

aagacaaagt ttagtttatc aaacaaagcc atggacaaag aaaagctgga gaaatatgaa     1920

aagggtaaat ttaatttcca aggggctgcc atgttcatgg ttcctgtgtc tattttagtc     1980

atactgaaca cattttgctt cctcggtggg ttttggaaag tgatcataat gaagaatatc     2040

ctggacatgt ttggacaact ttctctctct gcctacgttc tggttctcag ttgtccagtt     2100

cttgaaggga tgttaactag aatcagcaag aaaatggtct ga                        2142


<210>  26
<211>  713
<212>  PRT
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  An enzyme involved in making QA-mono from Quillaic acid (QsCSL1).

<400>  26

Met Lys Ser Pro Ser Asn Pro Asn Gln Lys Pro Ile Leu His Thr Cys 
1               5                   10                  15      


Thr Ile Gln Gln Pro Arg Ala Thr Leu Asn Lys Ile His Ser Leu Ile 
            20                  25                  30          


His Phe Ser Ala Ile Leu Val Leu Phe Tyr Tyr Arg Ile Thr Arg Leu 
        35                  40                  45              


Phe Phe Thr Asp Asp Phe Lys Val Pro Lys Leu Leu Trp Thr Leu Met 
    50                  55                  60                  


Thr Ile Ser Glu Phe Ile Leu Ala Phe Ile Trp Val Leu Ile Gln Pro 
65                  70                  75                  80  


Phe Arg Trp Arg Pro Val Ser Arg Ser Val Ile Pro Glu Asn Met Pro 
                85                  90                  95      


Lys Asp Ile Ser Leu Pro Ala Val Asp Val Phe Val Cys Thr Ala Asp 
            100                 105                 110         


Pro Gln Lys Glu Pro Thr Val Glu Val Met Asn Thr Ile Leu Ser Ala 
        115                 120                 125             


Met Ala Leu Asp Tyr Pro Ala Glu Lys Leu Ala Val Tyr Leu Ser Asp 
    130                 135                 140                 


Asp Gly Gly Ser Ala Val Thr Leu Tyr Ala Ile Lys Glu Ala Cys Cys 
145                 150                 155                 160 


Phe Ala Lys Met Trp Leu Pro Phe Cys Asn Lys Tyr Gly Ile Lys Ser 
                165                 170                 175     


Arg Cys Pro Glu Ala Tyr Phe Ser Lys Leu Ala Ala Asp Glu Trp Leu 
            180                 185                 190         


His Arg Ser Val Glu Phe Val Ala Glu Glu Lys Glu Val Lys Ala Asn 
        195                 200                 205             


Tyr Glu Glu Phe Lys Arg Asn Val Gln Lys Phe Gly Glu Gln Gln Glu 
    210                 215                 220                 


Asn Ser Arg Val Val His Asp Arg His Pro His Val Glu Ile Ile His 
225                 230                 235                 240 


Asn Asn Trp Asn Asn Glu Asp Gln Ala His Glu Met Pro Leu Leu Val 
                245                 250                 255     


Tyr Val Ser Arg Glu Arg Arg Pro Ser His His Pro Arg Phe Lys Ala 
            260                 265                 270         


Gly Ala Leu Asn Thr Leu Leu Arg Val Ser Gly Ile Ile Ser Asn Ser 
        275                 280                 285             


Pro Tyr Ile Leu Val Leu Asp Cys Asp Met Tyr Cys Asn Asp Pro Thr 
    290                 295                 300                 


Ser Ala Arg Gln Ala Met Cys Phe His Leu Asp Pro Gln Leu Ser Lys 
305                 310                 315                 320 


Asn Leu Ala Phe Val Gln Phe Pro Gln Ile Phe Tyr Asn Ala Ser Lys 
                325                 330                 335     


Asn Asp Val Tyr Asp Ala Gln Val Arg Ala Ala Tyr Gln Thr Lys Trp 
            340                 345                 350         


Gln Gly Met Asp Gly Leu Gln Gly Pro Ile Phe Ser Gly Thr Gly Phe 
        355                 360                 365             


Tyr Leu Lys Arg Lys Ala Met Tyr Gly Asn Pro Asp Gln Asp Asp Asn 
    370                 375                 380                 


Cys Leu Leu Lys Pro Tyr Lys Lys Phe Gly Met Ser Gly Glu Phe Val 
385                 390                 395                 400 


Glu Ser Leu Lys Val Leu Asn Glu Gln Asp Gly Thr Gln Lys Lys Leu 
                405                 410                 415     


Leu Asp Gly Phe Leu Gln Glu Ala Lys Leu Leu Ala Ser Cys Ala Tyr 
            420                 425                 430         


Glu Thr Lys Thr Ser Trp Gly Lys Glu Ile Gly Phe Ser Tyr Asp Cys 
        435                 440                 445             


Leu Ile Glu Ser Thr Phe Thr Gly Tyr Leu Leu His Cys Arg Gly Trp 
    450                 455                 460                 


Ile Ser Val Tyr Leu Tyr Pro Lys Arg Pro Cys Phe Leu Gly Cys Cys 
465                 470                 475                 480 


Pro Thr Asp Met Lys Asp Ala Met Val Gln Tyr Thr Lys Trp Met Ser 
                485                 490                 495     


Glu Leu Phe Ser Ile Ala Ile Ser Arg Phe Asn Pro Leu Leu Tyr Gly 
            500                 505                 510         


Val Ala Arg Met Ser Ile Leu Gln Ser Leu Cys Tyr Gly Ser Phe Thr 
        515                 520                 525             


Leu Ala Pro Ile Leu Ser Phe Pro Leu Phe Leu Tyr Gly Thr Val Pro 
    530                 535                 540                 


Gln Leu Cys Leu Leu Lys Gly Ile Ser Leu Phe Pro Lys Val Ser Asp 
545                 550                 555                 560 


Pro Trp Phe Ala Val Phe Ala Ala Ile Phe Val Ser Ser Leu Cys Gln 
                565                 570                 575     


His Trp Phe Glu Val Leu Ser Cys Asp Gly Thr Phe Thr Thr Trp Cys 
            580                 585                 590         


Asn Glu Gln Arg Ser Trp Leu Ile Lys Ser Val Ser Gly Ser Leu Phe 
        595                 600                 605             


Gly Val Val Gly Ala Ile Leu Gln Arg Leu Gly Leu Lys Thr Lys Phe 
    610                 615                 620                 


Ser Leu Ser Asn Lys Ala Met Asp Lys Glu Lys Leu Glu Lys Tyr Glu 
625                 630                 635                 640 


Lys Gly Lys Phe Asn Phe Gln Gly Ala Ala Met Phe Met Val Pro Val 
                645                 650                 655     


Ser Ile Leu Val Ile Leu Asn Thr Phe Cys Phe Leu Gly Gly Phe Trp 
            660                 665                 670         


Lys Val Ile Ile Met Lys Asn Ile Leu Asp Met Phe Gly Gln Leu Ser 
        675                 680                 685             


Leu Ser Ala Tyr Val Leu Val Leu Ser Cys Pro Val Leu Glu Gly Met 
    690                 695                 700                 


Leu Thr Arg Ile Ser Lys Lys Met Val 
705                 710             


<210>  27
<211>  2124
<212>  DNA
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  A nucleic acid molecule comprising a nucleotide which encodes the
       enzyme according to SEQ ID NO 28.

<400>  27
atggcgaccg tctcctccct ccacacttgc actgtacagc aaccccgtgc agccattaat       60

cgaattcaca ttttcttaca ctttattgcc atacttttcc tcttttacta ccgggtcacc      120

ggtcttttct atgacaatgc agtacccact ttagcttggt ctctaatgac cttagctgag      180

ttgattttcg ccttcgtttg ggtgctcagc caagccttcc ggtggcgccc ggtgttgcgt      240

tcagttattc ctgagaggat tcccaaagat gtacgattgc ccgcggtgga tatcttaatt      300

tgtacggctg acccattaaa ggaaccgacg gtggaggtga tgaacactgt cttgtccgcc      360

atggcattgg actatcctgc ggagaatctg gctgtatatc tttctgatga cgggggttct      420

ccggtcacct tatttgctat gaagcaagtg ggtccgtttg ctaagctgtg gcttccgttt      480

tgcaacaagt acggaatcaa aacaaggcat cctgagtctt ttttctcggc atttgcggat      540

gacgaaaggc ttcaccggag tgatgaattc agggcagagg aggaggcgat caaggacaaa      600

tatgaagaat ttaagagaac tatagagaaa tatggtggag aaggaaaaaa tagtcatgtt      660

gtacaagacc ggcctcctca tgtggagatt atacatgaca ctaggaagat tagagagaac      720

agtgaagacc aagctgtgcc tcttcttgtc tacgtctctc gtgagaaaag accatcctac      780

aattctcggt tcaaagcagg agctctgaac acccttcttc gagtttctgg ggtaatcagc      840

aatagcccat atgtattggt gttagactgt gacatgtact gcaatgatcc aacatcagct      900

agacaagcaa tgtgcttcca tcttgatcca caaatgtctc gcactctctc ttttgtacaa      960

ttcccccagg ttttctacaa tgttagtaaa aatgatatct atgatggcca agctagggca     1020

gcctttaaga caaagtggca aggtatggat ggactacgtg ggccactgct ttctggtact     1080

ggcttttatt tgaagaggaa gtccttgtat ggaagtccaa accaagaaga tgattgttta     1140

cttgagcccc ataagaattt tggaaagtgt gacaagctca tagaatcagt aaaggtcatt     1200

tatgaacgtg atgtttcaat aaaggcagat tcatcagatg ccattttgca agatgccaaa     1260

caattagcat cttgtcccta tgaaacaaac acaagctggg gcaaagaggt tgggttctcg     1320

tatgactgct tattagagag tacattcaca ggttatctgt tgcactgcag agggtggaca     1380

tcagtttatc tttatccaaa gaagccatgt ttcttagggt gtactccagt tgatatgaag     1440

gaagccatgg ttcagtatac gaagtggatt tctgaattat ttttacttgc tatctcaaga     1500

ttcaaccctc tgacatttgg gatatccaga atgtccattc tccagagcat gtgttacgga     1560

taccttacaa tcatgcccat tttatctgtt gctatgatct tctatgccac agttcctcaa     1620

ttgtgcctct tgagaggcgt acctctgttt cccaaggttt cagacccatg gtttgcagtg     1680

ttcctagcaa tatttgtgtc ctccctctgt cagcacttaa ttgaagtcct cacgagtgat     1740

ggcacgctca agacttggtg gaatgaacaa agaaattggg tgataaagtc tggttccggt     1800

agcgtatttg gagctctgag tggaatattg aagtggtttg gcatgaagat taaatttggt     1860

ttatcaaaca aagccgtgga caaagaaaag cttgagaaat atgaaaaggg taagtttgat     1920

ttccaagggg ctgccatgtt tatggttccc ttaactatat cagtcatctt gaacacatta     1980

tgccttatcg gtggtttatg gagagtaatc acacttaaaa acttcgaaga gatgtcaggg     2040

cagttcatca tctccttgta ctttctagct ctcagctatc caattcttga agggttacta     2100

agaaaaggca agggaaaggc ctaa                                            2124


<210>  28
<211>  707
<212>  PRT
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  An enzyme involved in making QA-mono from Quillaic acid 
       (QsCslG2).

<400>  28

Met Ala Thr Val Ser Ser Leu His Thr Cys Thr Val Gln Gln Pro Arg 
1               5                   10                  15      


Ala Ala Ile Asn Arg Ile His Ile Phe Leu His Phe Ile Ala Ile Leu 
            20                  25                  30          


Phe Leu Phe Tyr Tyr Arg Val Thr Gly Leu Phe Tyr Asp Asn Ala Val 
        35                  40                  45              


Pro Thr Leu Ala Trp Ser Leu Met Thr Leu Ala Glu Leu Ile Phe Ala 
    50                  55                  60                  


Phe Val Trp Val Leu Ser Gln Ala Phe Arg Trp Arg Pro Val Leu Arg 
65                  70                  75                  80  


Ser Val Ile Pro Glu Arg Ile Pro Lys Asp Val Arg Leu Pro Ala Val 
                85                  90                  95      


Asp Ile Leu Ile Cys Thr Ala Asp Pro Leu Lys Glu Pro Thr Val Glu 
            100                 105                 110         


Val Met Asn Thr Val Leu Ser Ala Met Ala Leu Asp Tyr Pro Ala Glu 
        115                 120                 125             


Asn Leu Ala Val Tyr Leu Ser Asp Asp Gly Gly Ser Pro Val Thr Leu 
    130                 135                 140                 


Phe Ala Met Lys Gln Val Gly Pro Phe Ala Lys Leu Trp Leu Pro Phe 
145                 150                 155                 160 


Cys Asn Lys Tyr Gly Ile Lys Thr Arg His Pro Glu Ser Phe Phe Ser 
                165                 170                 175     


Ala Phe Ala Asp Asp Glu Arg Leu His Arg Ser Asp Glu Phe Arg Ala 
            180                 185                 190         


Glu Glu Glu Ala Ile Lys Asp Lys Tyr Glu Glu Phe Lys Arg Thr Ile 
        195                 200                 205             


Glu Lys Tyr Gly Gly Glu Gly Lys Asn Ser His Val Val Gln Asp Arg 
    210                 215                 220                 


Pro Pro His Val Glu Ile Ile His Asp Thr Arg Lys Ile Arg Glu Asn 
225                 230                 235                 240 


Ser Glu Asp Gln Ala Val Pro Leu Leu Val Tyr Val Ser Arg Glu Lys 
                245                 250                 255     


Arg Pro Ser Tyr Asn Ser Arg Phe Lys Ala Gly Ala Leu Asn Thr Leu 
            260                 265                 270         


Leu Arg Val Ser Gly Val Ile Ser Asn Ser Pro Tyr Val Leu Val Leu 
        275                 280                 285             


Asp Cys Asp Met Tyr Cys Asn Asp Pro Thr Ser Ala Arg Gln Ala Met 
    290                 295                 300                 


Cys Phe His Leu Asp Pro Gln Met Ser Arg Thr Leu Ser Phe Val Gln 
305                 310                 315                 320 


Phe Pro Gln Val Phe Tyr Asn Val Ser Lys Asn Asp Ile Tyr Asp Gly 
                325                 330                 335     


Gln Ala Arg Ala Ala Phe Lys Thr Lys Trp Gln Gly Met Asp Gly Leu 
            340                 345                 350         


Arg Gly Pro Leu Leu Ser Gly Thr Gly Phe Tyr Leu Lys Arg Lys Ser 
        355                 360                 365             


Leu Tyr Gly Ser Pro Asn Gln Glu Asp Asp Cys Leu Leu Glu Pro His 
    370                 375                 380                 


Lys Asn Phe Gly Lys Cys Asp Lys Leu Ile Glu Ser Val Lys Val Ile 
385                 390                 395                 400 


Tyr Glu Arg Asp Val Ser Ile Lys Ala Asp Ser Ser Asp Ala Ile Leu 
                405                 410                 415     


Gln Asp Ala Lys Gln Leu Ala Ser Cys Pro Tyr Glu Thr Asn Thr Ser 
            420                 425                 430         


Trp Gly Lys Glu Val Gly Phe Ser Tyr Asp Cys Leu Leu Glu Ser Thr 
        435                 440                 445             


Phe Thr Gly Tyr Leu Leu His Cys Arg Gly Trp Thr Ser Val Tyr Leu 
    450                 455                 460                 


Tyr Pro Lys Lys Pro Cys Phe Leu Gly Cys Thr Pro Val Asp Met Lys 
465                 470                 475                 480 


Glu Ala Met Val Gln Tyr Thr Lys Trp Ile Ser Glu Leu Phe Leu Leu 
                485                 490                 495     


Ala Ile Ser Arg Phe Asn Pro Leu Thr Phe Gly Ile Ser Arg Met Ser 
            500                 505                 510         


Ile Leu Gln Ser Met Cys Tyr Gly Tyr Leu Thr Ile Met Pro Ile Leu 
        515                 520                 525             


Ser Val Ala Met Ile Phe Tyr Ala Thr Val Pro Gln Leu Cys Leu Leu 
    530                 535                 540                 


Arg Gly Val Pro Leu Phe Pro Lys Val Ser Asp Pro Trp Phe Ala Val 
545                 550                 555                 560 


Phe Leu Ala Ile Phe Val Ser Ser Leu Cys Gln His Leu Ile Glu Val 
                565                 570                 575     


Leu Thr Ser Asp Gly Thr Leu Lys Thr Trp Trp Asn Glu Gln Arg Asn 
            580                 585                 590         


Trp Val Ile Lys Ser Gly Ser Gly Ser Val Phe Gly Ala Leu Ser Gly 
        595                 600                 605             


Ile Leu Lys Trp Phe Gly Met Lys Ile Lys Phe Gly Leu Ser Asn Lys 
    610                 615                 620                 


Ala Val Asp Lys Glu Lys Leu Glu Lys Tyr Glu Lys Gly Lys Phe Asp 
625                 630                 635                 640 


Phe Gln Gly Ala Ala Met Phe Met Val Pro Leu Thr Ile Ser Val Ile 
                645                 650                 655     


Leu Asn Thr Leu Cys Leu Ile Gly Gly Leu Trp Arg Val Ile Thr Leu 
            660                 665                 670         


Lys Asn Phe Glu Glu Met Ser Gly Gln Phe Ile Ile Ser Leu Tyr Phe 
        675                 680                 685             


Leu Ala Leu Ser Tyr Pro Ile Leu Glu Gly Leu Leu Arg Lys Gly Lys 
    690                 695                 700                 


Gly Lys Ala 
705         


<210>  29
<211>  1479
<212>  DNA
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  A nucleic acid molecule comprising a nucleotide which encodes the
       enzyme according to SEQ ID NO 30.

<400>  29
atggtggagt ctccagcaga tcatgatgtg ctcaaaatca ttgtccttcc atgggtaacc       60

tcaggtcaca tgattcccat ggtagatgca gccagactat ttgctatgca tggtgcagat      120

gttaccatca tcaccacccc agctaatgcc cttacattcc agaaatccgt cgaccgtgat      180

ttcaattccg gtcgtttaat cagaactcac acccttaaat tccctgcagc agaagttggt      240

gtacctgaag gagttgaaaa cttcaacaat acttcccctg aaatgacctc caaagtctac      300

cttggagtct caatgctccg agaaccaacc caacaattga ttgaggatct gcgtccagat      360

tgtcttatca ctgatatgtt ctatccttgg gctgtggatg ttgctgacaa attaggcatt      420

ccaaggctaa tttttcaagg tcctggaagt tttggtttgt cagctatgca ttctatcaaa      480

cagtatgagc cctttaagtc agtaacttca gatactgaga cattcccact acctggattg      540

ccgcataagg tagagatgac aaggttgcag ataccaaaat gggttcgtga gccaaatggg      600

tacactcaat tgatgggcag ggtaaaagat tcggagagaa gaagctatgg gtcattggtg      660

aatagctttt atgacttcga aggcccttat gaagagcact ataggaaggc aacaggacag      720

agggtttgga gcattggacc agtttcagtt tgggtgaacc aagatgctgc agataaggtt      780

ggaagaggac aggatcttgt tgctgaagac caaaacagct ggttgaattg gctcaattcc      840

aaagagaaaa actctgttct gtatgtaagt tttgggagca tggccaagtt cccatctgct      900

cagcttcttg aaatagctca tgggcttgaa gcttcaggtc atagtttcat ctgggttgtc      960

agaaaagttg acggggatga tgatgtagac gtgtggcttc cagattttga gaagaaaatg     1020

aaagagaaca acaagggttt catcataagg aattgggcac cacaattgct catattggac     1080

catccagcaa ttggaggttt gctgaatcac agtggatgga attcagtact ggaaggtgct     1140

acagcaggct tgccaatgat cacttggcct ctgtatgccg agcattttta caatgaaagg     1200

ttggttctag atgtgttgaa aattggagta ccagttgggg tgaaggagtg gaagaacttg     1260

catgaggtgg gtgagttggt gagaagggat gcaattgcca aggcaattaa attgttaatg     1320

ggtagtggag aagaagctga ggtaatgagg aaaaaagcca aagagcttgg tgttggagca     1380

aagaaaggta ttcaggttgg aggttcttct cataccaatt tgatagcagt gattgatgag     1440

ttaaagtcac taaagaaatc aagaattcag ggtgtctga                            1479


<210>  30
<211>  492
<212>  PRT
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  An enzyme involved in making QA-Di from QA-Mono (Qs-3-O-GalT).

<400>  30

Met Val Glu Ser Pro Ala Asp His Asp Val Leu Lys Ile Ile Val Leu 
1               5                   10                  15      


Pro Trp Val Thr Ser Gly His Met Ile Pro Met Val Asp Ala Ala Arg 
            20                  25                  30          


Leu Phe Ala Met His Gly Ala Asp Val Thr Ile Ile Thr Thr Pro Ala 
        35                  40                  45              


Asn Ala Leu Thr Phe Gln Lys Ser Val Asp Arg Asp Phe Asn Ser Gly 
    50                  55                  60                  


Arg Leu Ile Arg Thr His Thr Leu Lys Phe Pro Ala Ala Glu Val Gly 
65                  70                  75                  80  


Val Pro Glu Gly Val Glu Asn Phe Asn Asn Thr Ser Pro Glu Met Thr 
                85                  90                  95      


Ser Lys Val Tyr Leu Gly Val Ser Met Leu Arg Glu Pro Thr Gln Gln 
            100                 105                 110         


Leu Ile Glu Asp Leu Arg Pro Asp Cys Leu Ile Thr Asp Met Phe Tyr 
        115                 120                 125             


Pro Trp Ala Val Asp Val Ala Asp Lys Leu Gly Ile Pro Arg Leu Ile 
    130                 135                 140                 


Phe Gln Gly Pro Gly Ser Phe Gly Leu Ser Ala Met His Ser Ile Lys 
145                 150                 155                 160 


Gln Tyr Glu Pro Phe Lys Ser Val Thr Ser Asp Thr Glu Thr Phe Pro 
                165                 170                 175     


Leu Pro Gly Leu Pro His Lys Val Glu Met Thr Arg Leu Gln Ile Pro 
            180                 185                 190         


Lys Trp Val Arg Glu Pro Asn Gly Tyr Thr Gln Leu Met Gly Arg Val 
        195                 200                 205             


Lys Asp Ser Glu Arg Arg Ser Tyr Gly Ser Leu Val Asn Ser Phe Tyr 
    210                 215                 220                 


Asp Phe Glu Gly Pro Tyr Glu Glu His Tyr Arg Lys Ala Thr Gly Gln 
225                 230                 235                 240 


Arg Val Trp Ser Ile Gly Pro Val Ser Val Trp Val Asn Gln Asp Ala 
                245                 250                 255     


Ala Asp Lys Val Gly Arg Gly Gln Asp Leu Val Ala Glu Asp Gln Asn 
            260                 265                 270         


Ser Trp Leu Asn Trp Leu Asn Ser Lys Glu Lys Asn Ser Val Leu Tyr 
        275                 280                 285             


Val Ser Phe Gly Ser Met Ala Lys Phe Pro Ser Ala Gln Leu Leu Glu 
    290                 295                 300                 


Ile Ala His Gly Leu Glu Ala Ser Gly His Ser Phe Ile Trp Val Val 
305                 310                 315                 320 


Arg Lys Val Asp Gly Asp Asp Asp Val Asp Val Trp Leu Pro Asp Phe 
                325                 330                 335     


Glu Lys Lys Met Lys Glu Asn Asn Lys Gly Phe Ile Ile Arg Asn Trp 
            340                 345                 350         


Ala Pro Gln Leu Leu Ile Leu Asp His Pro Ala Ile Gly Gly Leu Leu 
        355                 360                 365             


Asn His Ser Gly Trp Asn Ser Val Leu Glu Gly Ala Thr Ala Gly Leu 
    370                 375                 380                 


Pro Met Ile Thr Trp Pro Leu Tyr Ala Glu His Phe Tyr Asn Glu Arg 
385                 390                 395                 400 


Leu Val Leu Asp Val Leu Lys Ile Gly Val Pro Val Gly Val Lys Glu 
                405                 410                 415     


Trp Lys Asn Leu His Glu Val Gly Glu Leu Val Arg Arg Asp Ala Ile 
            420                 425                 430         


Ala Lys Ala Ile Lys Leu Leu Met Gly Ser Gly Glu Glu Ala Glu Val 
        435                 440                 445             


Met Arg Lys Lys Ala Lys Glu Leu Gly Val Gly Ala Lys Lys Gly Ile 
    450                 455                 460                 


Gln Val Gly Gly Ser Ser His Thr Asn Leu Ile Ala Val Ile Asp Glu 
465                 470                 475                 480 


Leu Lys Ser Leu Lys Lys Ser Arg Ile Gln Gly Val 
                485                 490         


<210>  31
<211>  1515
<212>  DNA
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  A nucleic acid molecule comprising a nucleotide which encodes the
       enzyme according to SEQ ID NO 32.

<400>  31
atggtctccg gcgacgacga tgtttctcgt cggccactga aagtttactt cattgcacac       60

ccctcacctg gccatattgc ccctctgacc aaaatagccc atctcttcgc tgccctcggt      120

gagcacgtga ctattctcac tactcccgcc aatgtccact tccatgagaa atccatcgac      180

aaaggaaagg cttccggcta tcatgttaac atccacaccg ttaaatttcc ttctaaagag      240

gtcggtctcc ctgacggcat cgaaaacttc tcttacgcct ccgatgttga aacagcagct      300

aaaatttggg ctggattcgc catgctacaa actgaaatgg agcaatatat ggagcttaac      360

ccacccgatt gcatcgttgc cgacatgttc acctcctgga cctccgactt tgctatcaaa      420

ttgggaatca caagaatcgt tttcaacgtc tattgtattt tcacacgctg tttggaagaa      480

gccatccgat caccggactc gccacacttg aacaaagaaa tctctgataa tgaaccgttt      540

gttatcccgg gtctaccaga ccccataaca attacccgag ctcaactgcc cgacggtacc      600

ttttctccca tgaaagaact agctagaaca gctgagttga agagctttgg aatggtgatc      660

aacgggtttt ccgaactcga aaccgattac atcgagcatt acaagaaaat catgggtcac      720

aaacggattt ggcatgtcgg accccttcag ctaatccacc gtaacgatga agacaaaatt      780

cagaggagcc acaagacagc ggtgctgagt gataacgata acgagttagt gagttggctt      840

aactcgaaga aacccgactc agttatttac atttgcttcg gtagtgcaac tcgtttctct      900

aatcaccagc tctatgaaat cgcctgtgga ttagaagctt ccgggcaccc atttttgtgg      960

ggcctacttt gggtgccaga agatgaagat aacgatgacg tgggcaacaa atggttgcca     1020

gctttcgaag aaagaattaa aaaggaaaat aagggaatga ttttaagggg gtgggctcca     1080

cagatgttaa tcttaaacca cccggcgatc ggtggtttca tgacgcattg tggttggaat     1140

gcggtggtgg aagcactttc attcggtgtt ccgactatta cgcttccagt tttctcggag     1200

cagttttata ctgagagact gatatcacaa gtgctcaaga ctggtgtgga ggttggtgca     1260

gagaagtgga cctatgcatt tgatgcgggg aaatatccgg tgagtaggga aaagatagcg     1320

acggcggtga agaagatatt agacgatgga gaagaggcag aaggaatgag aaagcgggcc     1380

agggagatga aagaaaaagc ccaaaaaagt gttgaagaag gtggatcctc ttataataat     1440

ttaacggcta tgattgaaga tcttaaagaa tttagggcta acaatggcaa ggctgcacaa     1500

gatcatgaat cgtga                                                      1515


<210>  32
<211>  504
<212>  PRT
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  An enzyme involved in making QA-TriR or QA-TriX from QA-Di 
       (Qs-3-O-RhaT/XylT).

<400>  32

Met Val Ser Gly Asp Asp Asp Val Ser Arg Arg Pro Leu Lys Val Tyr 
1               5                   10                  15      


Phe Ile Ala His Pro Ser Pro Gly His Ile Ala Pro Leu Thr Lys Ile 
            20                  25                  30          


Ala His Leu Phe Ala Ala Leu Gly Glu His Val Thr Ile Leu Thr Thr 
        35                  40                  45              


Pro Ala Asn Val His Phe His Glu Lys Ser Ile Asp Lys Gly Lys Ala 
    50                  55                  60                  


Ser Gly Tyr His Val Asn Ile His Thr Val Lys Phe Pro Ser Lys Glu 
65                  70                  75                  80  


Val Gly Leu Pro Asp Gly Ile Glu Asn Phe Ser Tyr Ala Ser Asp Val 
                85                  90                  95      


Glu Thr Ala Ala Lys Ile Trp Ala Gly Phe Ala Met Leu Gln Thr Glu 
            100                 105                 110         


Met Glu Gln Tyr Met Glu Leu Asn Pro Pro Asp Cys Ile Val Ala Asp 
        115                 120                 125             


Met Phe Thr Ser Trp Thr Ser Asp Phe Ala Ile Lys Leu Gly Ile Thr 
    130                 135                 140                 


Arg Ile Val Phe Asn Val Tyr Cys Ile Phe Thr Arg Cys Leu Glu Glu 
145                 150                 155                 160 


Ala Ile Arg Ser Pro Asp Ser Pro His Leu Asn Lys Glu Ile Ser Asp 
                165                 170                 175     


Asn Glu Pro Phe Val Ile Pro Gly Leu Pro Asp Pro Ile Thr Ile Thr 
            180                 185                 190         


Arg Ala Gln Leu Pro Asp Gly Thr Phe Ser Pro Met Lys Glu Leu Ala 
        195                 200                 205             


Arg Thr Ala Glu Leu Lys Ser Phe Gly Met Val Ile Asn Gly Phe Ser 
    210                 215                 220                 


Glu Leu Glu Thr Asp Tyr Ile Glu His Tyr Lys Lys Ile Met Gly His 
225                 230                 235                 240 


Lys Arg Ile Trp His Val Gly Pro Leu Gln Leu Ile His Arg Asn Asp 
                245                 250                 255     


Glu Asp Lys Ile Gln Arg Ser His Lys Thr Ala Val Leu Ser Asp Asn 
            260                 265                 270         


Asp Asn Glu Leu Val Ser Trp Leu Asn Ser Lys Lys Pro Asp Ser Val 
        275                 280                 285             


Ile Tyr Ile Cys Phe Gly Ser Ala Thr Arg Phe Ser Asn His Gln Leu 
    290                 295                 300                 


Tyr Glu Ile Ala Cys Gly Leu Glu Ala Ser Gly His Pro Phe Leu Trp 
305                 310                 315                 320 


Gly Leu Leu Trp Val Pro Glu Asp Glu Asp Asn Asp Asp Val Gly Asn 
                325                 330                 335     


Lys Trp Leu Pro Ala Phe Glu Glu Arg Ile Lys Lys Glu Asn Lys Gly 
            340                 345                 350         


Met Ile Leu Arg Gly Trp Ala Pro Gln Met Leu Ile Leu Asn His Pro 
        355                 360                 365             


Ala Ile Gly Gly Phe Met Thr His Cys Gly Trp Asn Ala Val Val Glu 
    370                 375                 380                 


Ala Leu Ser Phe Gly Val Pro Thr Ile Thr Leu Pro Val Phe Ser Glu 
385                 390                 395                 400 


Gln Phe Tyr Thr Glu Arg Leu Ile Ser Gln Val Leu Lys Thr Gly Val 
                405                 410                 415     


Glu Val Gly Ala Glu Lys Trp Thr Tyr Ala Phe Asp Ala Gly Lys Tyr 
            420                 425                 430         


Pro Val Ser Arg Glu Lys Ile Ala Thr Ala Val Lys Lys Ile Leu Asp 
        435                 440                 445             


Asp Gly Glu Glu Ala Glu Gly Met Arg Lys Arg Ala Arg Glu Met Lys 
    450                 455                 460                 


Glu Lys Ala Gln Lys Ser Val Glu Glu Gly Gly Ser Ser Tyr Asn Asn 
465                 470                 475                 480 


Leu Thr Ala Met Ile Glu Asp Leu Lys Glu Phe Arg Ala Asn Asn Gly 
                485                 490                 495     


Lys Ala Ala Gln Asp His Glu Ser 
            500                 


<210>  33
<211>  1485
<212>  DNA
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  A nucleic acid molecule comprising a nucleotide which encodes the
       enzyme according to SEQ ID NO 34.

<400>  33
atggtctccg gcgacgacga cgtttctcgt cggccactga aagtttactt tattgcacac       60

ccctcacctg gccatattgc ccctctaacc aaaatagccc aactctttgc tgcacgtggt      120

gagcacgtga ctattcttac tactcccgcc aatgtccact tccatgagaa atccatcgac      180

aaaggaaaga cttccggcta tcatgttaac atccacgccg ttaaatttcc ttctaaagag      240

gtcggtctcc ccgacggcat cgaaaacttc tctcacgcct ccgataatga aacagcagcc      300

aaaatttggg ccggattctc catgcttcaa actgaaatgg agcaatatat ggaacaaaac      360

ccacccgatt gcattgttgc cgacatgttc aaccgctgga cttccgactt cgctatcaaa      420

ttgggaatcc cgagaatagt tttcaacgtc tactgtattt tcacacgctg tttggaagaa      480

gcaatcagat cacctgactc gccacacttg aaactaaact ccgataatga acagtttatt      540

attccgggtc tacccgaccc cataacaatt acccgagctc aactgcccga cggtgccttt      600

tctgtcgtca aagaacaagt tagtgaagct gagttgaaaa gcttcggaat ggtgatcaac      660

gggttttccg aactcgaaac cgaatacatc gagtattaca agaatatcat gggtcgaaaa      720

cggatttggc atgtcggacc ccttcagctc atttaccaaa acgatgaccc caaagttcag      780

aggagccaga agacagcggt cgtgagtgac aacgagttag tgagttggct tgactcgaag      840

aaacccgact cagtgattta catttccttc ggtagtgcaa ttcgtttctc taataagcag      900

ctctatgaaa tagcatgtgg attagaagct tccggctacc catttttgtg ggccttactt      960

tgggtgccag aagatgacga cgacgtgggc aacaaatggt tgcctgattt cgaagaaaga     1020

ataaaaagag aaaataaggg aataattttc agggggtggg ccccacagat gttaatctta     1080

aaccacccgg cgatcggtgg tttcatgacg cattgtggtt ggaatgcggt ggtggaagcg     1140

ctttctttcg gtgttccgac tattacgctt ccggttttct cggagcagtt ttatactgag     1200

agactgatat cacaagtgct caagactggt gtcgaggtcg gtgcagagaa gtggacctat     1260

gcatttgatg cggggaaata tccggtgagt cgggaaaaga tagcgacggc ggtgaagaag     1320

atattagact gtggagaaga ggcagaagga atgagaaagc gggccaggga gatgaaagaa     1380

aaagcccaaa aaagtgttga agaaggtggg tcctcttata ataatttaac ggctatgatt     1440

gaagatctta aagaatttag ggctaacaat ggcaaggttg catga                     1485


<210>  34
<211>  494
<212>  PRT
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  An enzyme involved in making QA-TriR from QA-Di (Qs_0283850).

<400>  34

Met Val Ser Gly Asp Asp Asp Val Ser Arg Arg Pro Leu Lys Val Tyr 
1               5                   10                  15      


Phe Ile Ala His Pro Ser Pro Gly His Ile Ala Pro Leu Thr Lys Ile 
            20                  25                  30          


Ala Gln Leu Phe Ala Ala Arg Gly Glu His Val Thr Ile Leu Thr Thr 
        35                  40                  45              


Pro Ala Asn Val His Phe His Glu Lys Ser Ile Asp Lys Gly Lys Thr 
    50                  55                  60                  


Ser Gly Tyr His Val Asn Ile His Ala Val Lys Phe Pro Ser Lys Glu 
65                  70                  75                  80  


Val Gly Leu Pro Asp Gly Ile Glu Asn Phe Ser His Ala Ser Asp Asn 
                85                  90                  95      


Glu Thr Ala Ala Lys Ile Trp Ala Gly Phe Ser Met Leu Gln Thr Glu 
            100                 105                 110         


Met Glu Gln Tyr Met Glu Gln Asn Pro Pro Asp Cys Ile Val Ala Asp 
        115                 120                 125             


Met Phe Asn Arg Trp Thr Ser Asp Phe Ala Ile Lys Leu Gly Ile Pro 
    130                 135                 140                 


Arg Ile Val Phe Asn Val Tyr Cys Ile Phe Thr Arg Cys Leu Glu Glu 
145                 150                 155                 160 


Ala Ile Arg Ser Pro Asp Ser Pro His Leu Lys Leu Asn Ser Asp Asn 
                165                 170                 175     


Glu Gln Phe Ile Ile Pro Gly Leu Pro Asp Pro Ile Thr Ile Thr Arg 
            180                 185                 190         


Ala Gln Leu Pro Asp Gly Ala Phe Ser Val Val Lys Glu Gln Val Ser 
        195                 200                 205             


Glu Ala Glu Leu Lys Ser Phe Gly Met Val Ile Asn Gly Phe Ser Glu 
    210                 215                 220                 


Leu Glu Thr Glu Tyr Ile Glu Tyr Tyr Lys Asn Ile Met Gly Arg Lys 
225                 230                 235                 240 


Arg Ile Trp His Val Gly Pro Leu Gln Leu Ile Tyr Gln Asn Asp Asp 
                245                 250                 255     


Pro Lys Val Gln Arg Ser Gln Lys Thr Ala Val Val Ser Asp Asn Glu 
            260                 265                 270         


Leu Val Ser Trp Leu Asp Ser Lys Lys Pro Asp Ser Val Ile Tyr Ile 
        275                 280                 285             


Ser Phe Gly Ser Ala Ile Arg Phe Ser Asn Lys Gln Leu Tyr Glu Ile 
    290                 295                 300                 


Ala Cys Gly Leu Glu Ala Ser Gly Tyr Pro Phe Leu Trp Ala Leu Leu 
305                 310                 315                 320 


Trp Val Pro Glu Asp Asp Asp Asp Val Gly Asn Lys Trp Leu Pro Asp 
                325                 330                 335     


Phe Glu Glu Arg Ile Lys Arg Glu Asn Lys Gly Ile Ile Phe Arg Gly 
            340                 345                 350         


Trp Ala Pro Gln Met Leu Ile Leu Asn His Pro Ala Ile Gly Gly Phe 
        355                 360                 365             


Met Thr His Cys Gly Trp Asn Ala Val Val Glu Ala Leu Ser Phe Gly 
    370                 375                 380                 


Val Pro Thr Ile Thr Leu Pro Val Phe Ser Glu Gln Phe Tyr Thr Glu 
385                 390                 395                 400 


Arg Leu Ile Ser Gln Val Leu Lys Thr Gly Val Glu Val Gly Ala Glu 
                405                 410                 415     


Lys Trp Thr Tyr Ala Phe Asp Ala Gly Lys Tyr Pro Val Ser Arg Glu 
            420                 425                 430         


Lys Ile Ala Thr Ala Val Lys Lys Ile Leu Asp Cys Gly Glu Glu Ala 
        435                 440                 445             


Glu Gly Met Arg Lys Arg Ala Arg Glu Met Lys Glu Lys Ala Gln Lys 
    450                 455                 460                 


Ser Val Glu Glu Gly Gly Ser Ser Tyr Asn Asn Leu Thr Ala Met Ile 
465                 470                 475                 480 


Glu Asp Leu Lys Glu Phe Arg Ala Asn Asn Gly Lys Val Ala 
                485                 490                 


<210>  35
<211>  1491
<212>  DNA
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  A nucleic acid molecule comprising a nucleotide which encodes the
       enzyme according to SEQ ID NO 36.

<400>  35
atggtctccg gcgacgatac cgtttcacgg ccactgatag tttactttat tgcacacccc       60

tcacctggcc atattgcccc tctaaccaaa atagcccaac tcttcgctgc acgtggtgag      120

cacgtcacta ttcttactac tcccgccaat gtccacttcc atgagaaatc catcgacaaa      180

agaaagaatt ccggctatca tgttaacatc cacaccgtta aatttccttc taaagaggtc      240

ggtctccctg acggcatcga aaacttctct cacgcctccg ataatgaaac agcagccaaa      300

atttgggccg gattctccat gcttcaaact gaaatggagc aatatatgga acaaaaccca      360

cccgattgca tcgttgccga catgttcaac cgctggactt ccgacttcgc tatcaaattg      420

ggaatcccga gaatagtttt caacgtctac tgtattttca cacgctgttt ggaagaagca      480

atcagatcac ctgactcgcc acacttgaaa ctaaactccg ataatgaaca gtttattatt      540

cccggtctac ccgaccccat aacaattacc cgagctcaac tccccgacgg tgccttttct      600

gtcgtcaaag aacaagttag tgaagctgag ttgaaaagct tcggaatggt gatcaacggg      660

ttttccgaac tcgaaactga atacatcgag tattacaaga atatcatggg tcgcaaacgg      720

atttggcatg tcggacccct tcagctaatt taccaaaacg acgaccccaa agttcagagg      780

agccagaaga cagcggtctt gagtgacaac gagttagtga gttggcttga ctcgaagaaa      840

cccgactcag tgatttacat ttccttcggt agtgcaattc gtttctctaa taagcagctc      900

tatgaaatcg catgtggatt agaagcttcc ggctacccat ttttgtgggc cttactttgg      960

gtgccagaag atgatgacga cgtgggcaac aaatggttgc cgggtttcga agaaagaata     1020

aaaagagaaa ataagggaat aattttcagg gggtgggccc cacagatgtt aatcttaaac     1080

cacccggcga tcggtggttt catgacgcat tgtggttgga atgcggtggt ggaagcactt     1140

tcattcggtg ttccgactat tacgcttcca gttttctcgg agcagtttta tactgagaga     1200

ctgatatcac aagtgctcaa gactggtgtg gaggttggtg cagagaagtg gacctatgca     1260

tttgatgcgg ggaaatatcc ggtgagtagg gaaaagatag cgacggcggt gaagaagata     1320

ttagacgatg gagaagaggc agaaggaatg agaaagcggg ccagggagat gaaagaaaaa     1380

gcccaaaaaa gtgttgaaga aggtggatcc tcttataata atttaacggc tatgattgaa     1440

gatcttaaag aatttagggc taacaatggc aaggctgcaa tgaaatcatg a              1491


<210>  36
<211>  496
<212>  PRT
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  An enzyme involved in making QA-TriR from QA-Di 
       (DN20529_c0_g2_i8).

<400>  36

Met Val Ser Gly Asp Asp Thr Val Ser Arg Pro Leu Ile Val Tyr Phe 
1               5                   10                  15      


Ile Ala His Pro Ser Pro Gly His Ile Ala Pro Leu Thr Lys Ile Ala 
            20                  25                  30          


Gln Leu Phe Ala Ala Arg Gly Glu His Val Thr Ile Leu Thr Thr Pro 
        35                  40                  45              


Ala Asn Val His Phe His Glu Lys Ser Ile Asp Lys Arg Lys Asn Ser 
    50                  55                  60                  


Gly Tyr His Val Asn Ile His Thr Val Lys Phe Pro Ser Lys Glu Val 
65                  70                  75                  80  


Gly Leu Pro Asp Gly Ile Glu Asn Phe Ser His Ala Ser Asp Asn Glu 
                85                  90                  95      


Thr Ala Ala Lys Ile Trp Ala Gly Phe Ser Met Leu Gln Thr Glu Met 
            100                 105                 110         


Glu Gln Tyr Met Glu Gln Asn Pro Pro Asp Cys Ile Val Ala Asp Met 
        115                 120                 125             


Phe Asn Arg Trp Thr Ser Asp Phe Ala Ile Lys Leu Gly Ile Pro Arg 
    130                 135                 140                 


Ile Val Phe Asn Val Tyr Cys Ile Phe Thr Arg Cys Leu Glu Glu Ala 
145                 150                 155                 160 


Ile Arg Ser Pro Asp Ser Pro His Leu Lys Leu Asn Ser Asp Asn Glu 
                165                 170                 175     


Gln Phe Ile Ile Pro Gly Leu Pro Asp Pro Ile Thr Ile Thr Arg Ala 
            180                 185                 190         


Gln Leu Pro Asp Gly Ala Phe Ser Val Val Lys Glu Gln Val Ser Glu 
        195                 200                 205             


Ala Glu Leu Lys Ser Phe Gly Met Val Ile Asn Gly Phe Ser Glu Leu 
    210                 215                 220                 


Glu Thr Glu Tyr Ile Glu Tyr Tyr Lys Asn Ile Met Gly Arg Lys Arg 
225                 230                 235                 240 


Ile Trp His Val Gly Pro Leu Gln Leu Ile Tyr Gln Asn Asp Asp Pro 
                245                 250                 255     


Lys Val Gln Arg Ser Gln Lys Thr Ala Val Leu Ser Asp Asn Glu Leu 
            260                 265                 270         


Val Ser Trp Leu Asp Ser Lys Lys Pro Asp Ser Val Ile Tyr Ile Ser 
        275                 280                 285             


Phe Gly Ser Ala Ile Arg Phe Ser Asn Lys Gln Leu Tyr Glu Ile Ala 
    290                 295                 300                 


Cys Gly Leu Glu Ala Ser Gly Tyr Pro Phe Leu Trp Ala Leu Leu Trp 
305                 310                 315                 320 


Val Pro Glu Asp Asp Asp Asp Val Gly Asn Lys Trp Leu Pro Gly Phe 
                325                 330                 335     


Glu Glu Arg Ile Lys Arg Glu Asn Lys Gly Ile Ile Phe Arg Gly Trp 
            340                 345                 350         


Ala Pro Gln Met Leu Ile Leu Asn His Pro Ala Ile Gly Gly Phe Met 
        355                 360                 365             


Thr His Cys Gly Trp Asn Ala Val Val Glu Ala Leu Ser Phe Gly Val 
    370                 375                 380                 


Pro Thr Ile Thr Leu Pro Val Phe Ser Glu Gln Phe Tyr Thr Glu Arg 
385                 390                 395                 400 


Leu Ile Ser Gln Val Leu Lys Thr Gly Val Glu Val Gly Ala Glu Lys 
                405                 410                 415     


Trp Thr Tyr Ala Phe Asp Ala Gly Lys Tyr Pro Val Ser Arg Glu Lys 
            420                 425                 430         


Ile Ala Thr Ala Val Lys Lys Ile Leu Asp Asp Gly Glu Glu Ala Glu 
        435                 440                 445             


Gly Met Arg Lys Arg Ala Arg Glu Met Lys Glu Lys Ala Gln Lys Ser 
    450                 455                 460                 


Val Glu Glu Gly Gly Ser Ser Tyr Asn Asn Leu Thr Ala Met Ile Glu 
465                 470                 475                 480 


Asp Leu Lys Glu Phe Arg Ala Asn Asn Gly Lys Ala Ala Met Lys Ser 
                485                 490                 495     


<210>  37
<211>  1515
<212>  DNA
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  A nucleic acid molecule comprising a nucleotide which encodes the
       enzyme according to SEQ ID NO 38.

<400>  37
atggtctccg gcgacgacga tgtttctcgt cggccactga aagtttactt cattgcacac       60

ccctcacctg gccatattgc ccctctgacc aaaatagccc atctcttcgc tgccctcggt      120

gagcacgtga ctattctcac tactcccgcc aatgtccact tccatgagaa atccatcgac      180

aaaggaaagg cttccggcta tcatgttaac atccacaccg ttaaatttcc ttctaaagag      240

gtcggtctcc ctgacggcat cgaaaacttc tcttacgcct ccgatgttga aacagcagct      300

aaaatttggg ctggattcgc catgctacaa actgaaatgg agcaatatat ggagcttaac      360

ccacccgatt gcatcgttgc cgacatgttc acctcctgga cctccgactt tgctatcaaa      420

ttgggaatca caagaatcgt tttcaacgtc tattgtattt tcacacgctg tttggaagaa      480

gccatccgat caccggactc gccacacttg aacaaagaaa tctctgataa tgaaccgttt      540

gttatcccgg gtctaccaga ccccataaca attacccgag ctcaactgcc cgacggtacc      600

ttttctccca tgaaagaact agctagaaca gctgagttga agagctttgg aatggtgatc      660

aacgggtttt ccgaactcga aaccgattac atcgagcatt acaagaaaat catgggtcac      720

aaacggattt ggcatgtcgg accccttcag ctaatccacc gtaacgatga agacaaaatt      780

cagaggagcc acaagacagc ggtgctgagt gataacgata acgagttagt gagttggctt      840

aactcgaaga aacccgactc agttatttac atttgcttcg gtagtgcaac tcgtttctct      900

aatcaccagc tctatgaaat cgcctgtgga ttagaagctt ccgggcaccc atttttgtgg      960

ggcctacttt gggtgccaga agatgaagat aacgatgacg tgggcaacaa atggttgcca     1020

gctttcgaag aaagaattaa aaaggaaaat aagggaatga ttttaagggg gtgggctcca     1080

cagatgttaa tcttgaatca cccggcgatc ggtggtttca tgacgcattg tggttggaat     1140

gcggcggtgg aggcgctttc ttccggtgtt ccgattatta catttccggt tttctcggat     1200

cagttttata atgaaaggct gatatcacaa gtgcataagt gtggggtggg ggttggtacg     1260

gaggcgtgga gctatgcatt cgatgccggg aagaatccgg tgggtcggga aaagataatg     1320

acggcggtga agaagatatt agacggtgga gaagaggcgg aaggaatgag aaagagggcc     1380

cgggagctga aagaaatagc taaaagaagt gtggaagaag gtgggtcctc ttataataat     1440

ttaacggcta tgattcaaga tctgaaagaa tttagagcta acaatggcaa ggctgcacaa     1500

gatcatgaat cgtga                                                      1515


<210>  38
<211>  504
<212>  PRT
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  An enzyme involved in making QA-TriX from QA-Di (Qs_0283870).

<400>  38

Met Val Ser Gly Asp Asp Asp Val Ser Arg Arg Pro Leu Lys Val Tyr 
1               5                   10                  15      


Phe Ile Ala His Pro Ser Pro Gly His Ile Ala Pro Leu Thr Lys Ile 
            20                  25                  30          


Ala His Leu Phe Ala Ala Leu Gly Glu His Val Thr Ile Leu Thr Thr 
        35                  40                  45              


Pro Ala Asn Val His Phe His Glu Lys Ser Ile Asp Lys Gly Lys Ala 
    50                  55                  60                  


Ser Gly Tyr His Val Asn Ile His Thr Val Lys Phe Pro Ser Lys Glu 
65                  70                  75                  80  


Val Gly Leu Pro Asp Gly Ile Glu Asn Phe Ser Tyr Ala Ser Asp Val 
                85                  90                  95      


Glu Thr Ala Ala Lys Ile Trp Ala Gly Phe Ala Met Leu Gln Thr Glu 
            100                 105                 110         


Met Glu Gln Tyr Met Glu Leu Asn Pro Pro Asp Cys Ile Val Ala Asp 
        115                 120                 125             


Met Phe Thr Ser Trp Thr Ser Asp Phe Ala Ile Lys Leu Gly Ile Thr 
    130                 135                 140                 


Arg Ile Val Phe Asn Val Tyr Cys Ile Phe Thr Arg Cys Leu Glu Glu 
145                 150                 155                 160 


Ala Ile Arg Ser Pro Asp Ser Pro His Leu Asn Lys Glu Ile Ser Asp 
                165                 170                 175     


Asn Glu Pro Phe Val Ile Pro Gly Leu Pro Asp Pro Ile Thr Ile Thr 
            180                 185                 190         


Arg Ala Gln Leu Pro Asp Gly Thr Phe Ser Pro Met Lys Glu Leu Ala 
        195                 200                 205             


Arg Thr Ala Glu Leu Lys Ser Phe Gly Met Val Ile Asn Gly Phe Ser 
    210                 215                 220                 


Glu Leu Glu Thr Asp Tyr Ile Glu His Tyr Lys Lys Ile Met Gly His 
225                 230                 235                 240 


Lys Arg Ile Trp His Val Gly Pro Leu Gln Leu Ile His Arg Asn Asp 
                245                 250                 255     


Glu Asp Lys Ile Gln Arg Ser His Lys Thr Ala Val Leu Ser Asp Asn 
            260                 265                 270         


Asp Asn Glu Leu Val Ser Trp Leu Asn Ser Lys Lys Pro Asp Ser Val 
        275                 280                 285             


Ile Tyr Ile Cys Phe Gly Ser Ala Thr Arg Phe Ser Asn His Gln Leu 
    290                 295                 300                 


Tyr Glu Ile Ala Cys Gly Leu Glu Ala Ser Gly His Pro Phe Leu Trp 
305                 310                 315                 320 


Gly Leu Leu Trp Val Pro Glu Asp Glu Asp Asn Asp Asp Val Gly Asn 
                325                 330                 335     


Lys Trp Leu Pro Ala Phe Glu Glu Arg Ile Lys Lys Glu Asn Lys Gly 
            340                 345                 350         


Met Ile Leu Arg Gly Trp Ala Pro Gln Met Leu Ile Leu Asn His Pro 
        355                 360                 365             


Ala Ile Gly Gly Phe Met Thr His Cys Gly Trp Asn Ala Ala Val Glu 
    370                 375                 380                 


Ala Leu Ser Ser Gly Val Pro Ile Ile Thr Phe Pro Val Phe Ser Asp 
385                 390                 395                 400 


Gln Phe Tyr Asn Glu Arg Leu Ile Ser Gln Val His Lys Cys Gly Val 
                405                 410                 415     


Gly Val Gly Thr Glu Ala Trp Ser Tyr Ala Phe Asp Ala Gly Lys Asn 
            420                 425                 430         


Pro Val Gly Arg Glu Lys Ile Met Thr Ala Val Lys Lys Ile Leu Asp 
        435                 440                 445             


Gly Gly Glu Glu Ala Glu Gly Met Arg Lys Arg Ala Arg Glu Leu Lys 
    450                 455                 460                 


Glu Ile Ala Lys Arg Ser Val Glu Glu Gly Gly Ser Ser Tyr Asn Asn 
465                 470                 475                 480 


Leu Thr Ala Met Ile Gln Asp Leu Lys Glu Phe Arg Ala Asn Asn Gly 
                485                 490                 495     


Lys Ala Ala Gln Asp His Glu Ser 
            500                 


<210>  39
<211>  1053
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Acanthocystis turfacea chlorella virus 1 UDP-d-glucose 
       4,6-dehydratase (ATCV-1) coding sequence (1053 bp): NB: This 
       sequence was codon-optimised for expression in N. benthamiana. 
       The original sequence can be found as Genbank ID: NC_008724.1

<400>  39
atgaatagtc aggagtatac tcctaaatca gtccttgtaa ctggtggggc aggtttcatt       60

ggcagccatg tcgtgatgaa attggtccag aggtatccgg aatgcaaggt cgttgttctt      120

gacaaaatgg attactgcgc taccttaaat aatcttgcta cggtacgaga tgcccccaat      180

tttaaatttg taaaaggtga tatacaaagc actgatcttt tagctcacgt gctcaaacag      240

gaaaagatag ataccattat gcactttgcc gcccagaccc atgtagataa tagctttggc      300

aatagtttag catttacgat gaacaacgta tacggcactc acgtccttct cgaatgtgcc      360

cgattgtatg gtggtgttca gagattcatt aacgtgtcaa ctgatgaggt ctacggtgag      420

agttccttgg gaaagaagga ggggttggac gaacactcct ccctcgaacc gacaaatcct      480

tatgccgccg caaaggctgg agctgaaatg atggctagag catatcatac gtcatacaaa      540

ctcccggtaa tagtcacgcg tggcaataat gtatatggtc cgcaccagtt ccccgaaaaa      600

atgatcccca aatttattct cagggcaacc agaggcctcg atttgccaat acatggtgat      660

ggcggcgccc tgcgatcata cctttacgtc gatgatgttg ccgaagctta tattacaatt      720

cttttaaagg gcaatgttgg agagacctat aacatcggca cgcaaaagga gcgatccgtt      780

gtggacgtgg cccatgacat ctgcaagatt tttaaccgag actcagatac agctatatgg      840

cacgttaaag accgagcctt caatgatcga cgttatttta tttctgataa aaaattactt      900

gacctcggct ggcaagaaaa aaccacctgg gaggacggtc ttaagcaaac tgtagggtgg      960

tatttgcaac atgcaactag gtcctactgg gatcacggca acatggaatt agctttagac     1020

gcccatccga cacttcaagt tcctaaattc taa                                  1053


<210>  40
<211>  350
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Acanthocystis turfacea chlorella virus 1 UDP-d-glucose 
       4,6-dehydratase (ATCV-1) translated nucleotide sequence (350 aa)

<400>  40

Met Asn Ser Gln Glu Tyr Thr Pro Lys Ser Val Leu Val Thr Gly Gly 
1               5                   10                  15      


Ala Gly Phe Ile Gly Ser His Val Val Met Lys Leu Val Gln Arg Tyr 
            20                  25                  30          


Pro Glu Cys Lys Val Val Val Leu Asp Lys Met Asp Tyr Cys Ala Thr 
        35                  40                  45              


Leu Asn Asn Leu Ala Thr Val Arg Asp Ala Pro Asn Phe Lys Phe Val 
    50                  55                  60                  


Lys Gly Asp Ile Gln Ser Thr Asp Leu Leu Ala His Val Leu Lys Gln 
65                  70                  75                  80  


Glu Lys Ile Asp Thr Ile Met His Phe Ala Ala Gln Thr His Val Asp 
                85                  90                  95      


Asn Ser Phe Gly Asn Ser Leu Ala Phe Thr Met Asn Asn Val Tyr Gly 
            100                 105                 110         


Thr His Val Leu Leu Glu Cys Ala Arg Leu Tyr Gly Gly Val Gln Arg 
        115                 120                 125             


Phe Ile Asn Val Ser Thr Asp Glu Val Tyr Gly Glu Ser Ser Leu Gly 
    130                 135                 140                 


Lys Lys Glu Gly Leu Asp Glu His Ser Ser Leu Glu Pro Thr Asn Pro 
145                 150                 155                 160 


Tyr Ala Ala Ala Lys Ala Gly Ala Glu Met Met Ala Arg Ala Tyr His 
                165                 170                 175     


Thr Ser Tyr Lys Leu Pro Val Ile Val Thr Arg Gly Asn Asn Val Tyr 
            180                 185                 190         


Gly Pro His Gln Phe Pro Glu Lys Met Ile Pro Lys Phe Ile Leu Arg 
        195                 200                 205             


Ala Thr Arg Gly Leu Asp Leu Pro Ile His Gly Asp Gly Gly Ala Leu 
    210                 215                 220                 


Arg Ser Tyr Leu Tyr Val Asp Asp Val Ala Glu Ala Tyr Ile Thr Ile 
225                 230                 235                 240 


Leu Leu Lys Gly Asn Val Gly Glu Thr Tyr Asn Ile Gly Thr Gln Lys 
                245                 250                 255     


Glu Arg Ser Val Val Asp Val Ala His Asp Ile Cys Lys Ile Phe Asn 
            260                 265                 270         


Arg Asp Ser Asp Thr Ala Ile Trp His Val Lys Asp Arg Ala Phe Asn 
        275                 280                 285             


Asp Arg Arg Tyr Phe Ile Ser Asp Lys Lys Leu Leu Asp Leu Gly Trp 
    290                 295                 300                 


Gln Glu Lys Thr Thr Trp Glu Asp Gly Leu Lys Gln Thr Val Gly Trp 
305                 310                 315                 320 


Tyr Leu Gln His Ala Thr Arg Ser Tyr Trp Asp His Gly Asn Met Glu 
                325                 330                 335     


Leu Ala Leu Asp Ala His Pro Thr Leu Gln Val Pro Lys Phe 
            340                 345                 350 


<210>  41
<211>  693
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aggregatibacter actinomycetemcomitans NDP-4-keto-6-deoxy-glucose 
       4-ketoreductase (AaFCD) coding sequence (693 bp): NB: This 
       sequence was codon-optimised for expression in N. benthamiana. 
       The original sequence can be found as Genbank ID: AB002668.1

<400>  41
atgataattg gcaacggtat gctggcaaaa gcctttgaat cattccataa gcgaacttac       60

aattacataa tatttgcatc cggagtgagc aactcaaacg aaacttcctt cgagaatttc      120

aacagagaga aggaattgct tcttgaagtc ctggagcaat ataaagacaa aactatcgtt      180

tactttagtt cctgctccat atacgattct agtttgacga attctttgta tgtctaccac      240

aaaatgtgta tggagagact ggtgcgtgaa aactccaaga attatctcat agcccgtctc      300

ccccaagtta ttggtaaaac gtattcacca accattgtca acttcctttt taacaaaatc      360

aaaaataggg agtgtttcag catattcgga aaagctcacc gaaattttat cgacgtggat      420

gatgtcgtta aggtcaccaa ttacttattg aaggagggtc tgttcattaa cagtattgtg      480

aacttggcaa gcacgcacca tacctccatg tacgaattaa tcttatattt ggaaaaaata      540

agtaatcaac gtgccttcta taatgttgag aacaaagggt ctaggtactt tattgatgtt      600

tcaatactgc aggatgttta tcagaagctg gggatcaaat ttgacaaaga ttacgtagaa      660

aaggttatca acaagtacta cgctattaag taa                                   693


<210>  42
<211>  230
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aggregatibacter actinomycetemcomitans NDP-4-keto-6-deoxy-glucose 
       4-ketoreductase (AaFCD) translated nucleotide sequence (230 aa)

<400>  42

Met Ile Ile Gly Asn Gly Met Leu Ala Lys Ala Phe Glu Ser Phe His 
1               5                   10                  15      


Lys Arg Thr Tyr Asn Tyr Ile Ile Phe Ala Ser Gly Val Ser Asn Ser 
            20                  25                  30          


Asn Glu Thr Ser Phe Glu Asn Phe Asn Arg Glu Lys Glu Leu Leu Leu 
        35                  40                  45              


Glu Val Leu Glu Gln Tyr Lys Asp Lys Thr Ile Val Tyr Phe Ser Ser 
    50                  55                  60                  


Cys Ser Ile Tyr Asp Ser Ser Leu Thr Asn Ser Leu Tyr Val Tyr His 
65                  70                  75                  80  


Lys Met Cys Met Glu Arg Leu Val Arg Glu Asn Ser Lys Asn Tyr Leu 
                85                  90                  95      


Ile Ala Arg Leu Pro Gln Val Ile Gly Lys Thr Tyr Ser Pro Thr Ile 
            100                 105                 110         


Val Asn Phe Leu Phe Asn Lys Ile Lys Asn Arg Glu Cys Phe Ser Ile 
        115                 120                 125             


Phe Gly Lys Ala His Arg Asn Phe Ile Asp Val Asp Asp Val Val Lys 
    130                 135                 140                 


Val Thr Asn Tyr Leu Leu Lys Glu Gly Leu Phe Ile Asn Ser Ile Val 
145                 150                 155                 160 


Asn Leu Ala Ser Thr His His Thr Ser Met Tyr Glu Leu Ile Leu Tyr 
                165                 170                 175     


Leu Glu Lys Ile Ser Asn Gln Arg Ala Phe Tyr Asn Val Glu Asn Lys 
            180                 185                 190         


Gly Ser Arg Tyr Phe Ile Asp Val Ser Ile Leu Gln Asp Val Tyr Gln 
        195                 200                 205             


Lys Leu Gly Ile Lys Phe Asp Lys Asp Tyr Val Glu Lys Val Ile Asn 
    210                 215                 220                 


Lys Tyr Tyr Ala Ile Lys 
225                 230 


<210>  43
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anoxybacillus tepidamans NDP-4-keto-6-deoxy-glucose 
       4-ketoreductase (AtFCD) coding sequence (927 bp): NB: This 
       sequence was codon-optimised for expression in N. benthamiana. 
       The original sequence can be found as Genbank ID: AY883421.5 
       

<400>  43
atgaagagga tactgatact cggatgcggt tacctgggtt taaatctcgc aaactatttt       60

tgtaaaaaaa attatgatgt ctcagtgata gggagaaagt ctgtctatag caattttttg      120

gaagaggaga tagagttcat agaagatgat atcaaaaata taaatagtta taagcacatg      180

tttaatgagg agacaaccgt catttacgcc ataggaagta ttaacgcaaa taactatttt      240

atggacctga ggaatgatat agaaaactca tacatcccct tcattaacct ccttaacttt      300

ctttccgaaa agtatattca aaagttcgtc tttctctctt cagccggaac agtctatggg      360

aacgtgaata agaattatat aagcgagaat gagattctta acccaatttc aatctatggt      420

ttgcagaaag ccttctttga acaactgata aggattaaaa acaatgaggc tagccatttc      480

aggtatttga tcttcagaat atctaacccc tatgggggaa tcaacattcc gaacaagaat      540

cagggaatta ttccgacgtt agtgtacaaa gccgtgaaca atgagccttt cgaactttgg      600

gcatcaatca ataccatccg tgattatatt tacatcgatg accttagcga attgatctac      660

aaaacaatct atctggacat ttataacgag accctcaatc tcgggtccgg taaaggaaca      720

tcaatcaagc aactcattag cctcgtggag gagattttgg gaaagaagat cactattctt      780

gaaaagcccc ccataaagac taacgttttg aaaaatatac ttgatatttc taagctcgtc      840

aacaccgtag gctacgaacc aaagatcagc attgaagagg gtattagccg ttacatcaac      900

actattttaa cgaagaacat tttttaa                                          927


<210>  44
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anoxybacillus tepidamans NDP-4-keto-6-deoxy-glucose 
       4-ketoreductase (AtFCD) translated nucleotide sequence (308 aa)

<400>  44

Met Lys Arg Ile Leu Ile Leu Gly Cys Gly Tyr Leu Gly Leu Asn Leu 
1               5                   10                  15      


Ala Asn Tyr Phe Cys Lys Lys Asn Tyr Asp Val Ser Val Ile Gly Arg 
            20                  25                  30          


Lys Ser Val Tyr Ser Asn Phe Leu Glu Glu Glu Ile Glu Phe Ile Glu 
        35                  40                  45              


Asp Asp Ile Lys Asn Ile Asn Ser Tyr Lys His Met Phe Asn Glu Glu 
    50                  55                  60                  


Thr Thr Val Ile Tyr Ala Ile Gly Ser Ile Asn Ala Asn Asn Tyr Phe 
65                  70                  75                  80  


Met Asp Leu Arg Asn Asp Ile Glu Asn Ser Tyr Ile Pro Phe Ile Asn 
                85                  90                  95      


Leu Leu Asn Phe Leu Ser Glu Lys Tyr Ile Gln Lys Phe Val Phe Leu 
            100                 105                 110         


Ser Ser Ala Gly Thr Val Tyr Gly Asn Val Asn Lys Asn Tyr Ile Ser 
        115                 120                 125             


Glu Asn Glu Ile Leu Asn Pro Ile Ser Ile Tyr Gly Leu Gln Lys Ala 
    130                 135                 140                 


Phe Phe Glu Gln Leu Ile Arg Ile Lys Asn Asn Glu Ala Ser His Phe 
145                 150                 155                 160 


Arg Tyr Leu Ile Phe Arg Ile Ser Asn Pro Tyr Gly Gly Ile Asn Ile 
                165                 170                 175     


Pro Asn Lys Asn Gln Gly Ile Ile Pro Thr Leu Val Tyr Lys Ala Val 
            180                 185                 190         


Asn Asn Glu Pro Phe Glu Leu Trp Ala Ser Ile Asn Thr Ile Arg Asp 
        195                 200                 205             


Tyr Ile Tyr Ile Asp Asp Leu Ser Glu Leu Ile Tyr Lys Thr Ile Tyr 
    210                 215                 220                 


Leu Asp Ile Tyr Asn Glu Thr Leu Asn Leu Gly Ser Gly Lys Gly Thr 
225                 230                 235                 240 


Ser Ile Lys Gln Leu Ile Ser Leu Val Glu Glu Ile Leu Gly Lys Lys 
                245                 250                 255     


Ile Thr Ile Leu Glu Lys Pro Pro Ile Lys Thr Asn Val Leu Lys Asn 
            260                 265                 270         


Ile Leu Asp Ile Ser Lys Leu Val Asn Thr Val Gly Tyr Glu Pro Lys 
        275                 280                 285             


Ile Ser Ile Glu Glu Gly Ile Ser Arg Tyr Ile Asn Thr Ile Leu Thr 
    290                 295                 300                 


Lys Asn Ile Phe 
305             


<210>  45
<211>  951
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Escherichia coli NDP-4-keto-6-deoxy-glucose 4-ketoreductase 
       (EcFCD)  coding sequence (951 bp): NB: This sequence was 
       codon-optimised for expression in N. benthamiana. The original 
       sequence can be found as Genbank ID: AY528413.1 
       

<400>  45
atggatgctc gtaaaaatgg ggtattaata accggtggag ctgggttcat aggtaaagcc       60

ttaataactg aaatggtcga acgtcaaatt cccctggtgt catttgacat cagcgataag      120

cccgacagtt tgccagagct ttccgaatat ttcaactggt ataaattctc ataccttgag      180

agttcacaga ggattaaaga gcttcacgaa atagtttcca ggcataacat caaaacggtc      240

atccatttag ctacaactat gtttccccac gaatccaaaa agaacatcga taaggattgc      300

ttagaaaacg tttatgccaa cgtgtgtttc tttaagaatt tatatgaaaa cggctgtgaa      360

aaaattatct tcgcctcatc aggtggcacc gtatatggga agtctgatac acccttctcc      420

gaagacgatg ccctgcttcc cgaaatttcc tacggactgt ccaaggttat gactgaaact      480

tatctccgat tcatagccaa ggaattgaat gggaagtcca tctctctcag aatatctaac      540

ccctatggtg aggggcaaag gattgacggg aaacaaggag tcattccaat tttcctcaat      600

aaaatcagca acgacatccc catcgacatc attggctcta tcgaatcaaa gcgagactac      660

atttatattt cagatctcgt acaagctttc atgtgctctc tggaatatga aggtcacgaa      720

gacatattta atataggttc tggggaaagc ataactctga agaaattgat cgagacgatt      780

gagttcaagc tgaacaagaa ggctgtgatt ggatttcaag atccgatcca caccaatgcc      840

aatggtataa ttctcgacat caaacgagcc atggcagaac tcggctggag gcccaccgtg      900

gtcctggatg atggcatcga taaattaatc aagagcattc gatgcaagta a               951


<210>  46
<211>  316
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Echerichia coli NDP-4-keto-6-deoxy-glucose 4-ketoreductase 
       (EcFCD) translated nucleotide sequence (316 aa)

<400>  46

Met Asp Ala Arg Lys Asn Gly Val Leu Ile Thr Gly Gly Ala Gly Phe 
1               5                   10                  15      


Ile Gly Lys Ala Leu Ile Thr Glu Met Val Glu Arg Gln Ile Pro Leu 
            20                  25                  30          


Val Ser Phe Asp Ile Ser Asp Lys Pro Asp Ser Leu Pro Glu Leu Ser 
        35                  40                  45              


Glu Tyr Phe Asn Trp Tyr Lys Phe Ser Tyr Leu Glu Ser Ser Gln Arg 
    50                  55                  60                  


Ile Lys Glu Leu His Glu Ile Val Ser Arg His Asn Ile Lys Thr Val 
65                  70                  75                  80  


Ile His Leu Ala Thr Thr Met Phe Pro His Glu Ser Lys Lys Asn Ile 
                85                  90                  95      


Asp Lys Asp Cys Leu Glu Asn Val Tyr Ala Asn Val Cys Phe Phe Lys 
            100                 105                 110         


Asn Leu Tyr Glu Asn Gly Cys Glu Lys Ile Ile Phe Ala Ser Ser Gly 
        115                 120                 125             


Gly Thr Val Tyr Gly Lys Ser Asp Thr Pro Phe Ser Glu Asp Asp Ala 
    130                 135                 140                 


Leu Leu Pro Glu Ile Ser Tyr Gly Leu Ser Lys Val Met Thr Glu Thr 
145                 150                 155                 160 


Tyr Leu Arg Phe Ile Ala Lys Glu Leu Asn Gly Lys Ser Ile Ser Leu 
                165                 170                 175     


Arg Ile Ser Asn Pro Tyr Gly Glu Gly Gln Arg Ile Asp Gly Lys Gln 
            180                 185                 190         


Gly Val Ile Pro Ile Phe Leu Asn Lys Ile Ser Asn Asp Ile Pro Ile 
        195                 200                 205             


Asp Ile Ile Gly Ser Ile Glu Ser Lys Arg Asp Tyr Ile Tyr Ile Ser 
    210                 215                 220                 


Asp Leu Val Gln Ala Phe Met Cys Ser Leu Glu Tyr Glu Gly His Glu 
225                 230                 235                 240 


Asp Ile Phe Asn Ile Gly Ser Gly Glu Ser Ile Thr Leu Lys Lys Leu 
                245                 250                 255     


Ile Glu Thr Ile Glu Phe Lys Leu Asn Lys Lys Ala Val Ile Gly Phe 
            260                 265                 270         


Gln Asp Pro Ile His Thr Asn Ala Asn Gly Ile Ile Leu Asp Ile Lys 
        275                 280                 285             


Arg Ala Met Ala Glu Leu Gly Trp Arg Pro Thr Val Val Leu Asp Asp 
    290                 295                 300                 


Gly Ile Asp Lys Leu Ile Lys Ser Ile Arg Cys Lys 
305                 310                 315     


<210>  47
<211>  897
<212>  DNA
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  A nucleic acid molecule comprising a nucleotide which encodes the
       QsFSL-1 enzyme according to SEQ ID NO 48. QsFSL-1

<400>  47
atggcagaag caacagagag gtatgctgtt gtgacaggat ctaataaagg aattggattt       60

gggatatgca agcagctggc ttctaagggg attacagtag tgctaacagc tagagatgat      120

aagagaggtc ttgaagcagt tgagaaattg aaagaatttg atctgcatgg tcatgtgctt      180

tttcatcaac ttgatgtgtc tgatacagct agtgttacta gccttgcaga ttttatcaaa      240

acccagtttg ggaaactaga tatcttggta aacaatgcag gtataactgg aaccactgta      300

gatgctgatg ctttagcatc ttcaggctat ggtactgggg gtgaacgtaa acctattgat      360

tggaataaaa tagtgataga gacttatgaa tcagttgaaa aagctatcaa caccaactat      420

tatggagcca aaagaatggc tgaagcactt ataccccttc ttcaagtatc agactcacca      480

aggattgtta atgcttcctc tcctatggca aagctagaga atattccaag tggatggggt      540

aaggaagtgc taagtgatgt tgatagccta acagaagaga aacttgatga gatgttgacc      600

caattattga aagatttcaa agagggttca ttagaaacca aaggctggcc tactcttatg      660

tcttcgtata taatctcaaa agctgcttta aatgcctaca caaggattct tgctaagaag      720

tacccatctt tctgcatcaa ttgtgtagac cctggtcatg tgaagactga cataaatcgt      780

cacaccggcc acttaagtat tgatgaaggt gctgaaagcc atgtgagatt ggccctgctg      840

cctgatggtg gcccttctgg acatttcttc tccaggactg aagagacacc attttga         897


<210>  48
<211>  298
<212>  PRT
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  An oxidoreductase enzyme capable of enhancing the activity of a 
       fucosyltransferase (QsFSL-1)

<400>  48

Met Ala Glu Ala Thr Glu Arg Tyr Ala Val Val Thr Gly Ser Asn Lys 
1               5                   10                  15      


Gly Ile Gly Phe Gly Ile Cys Lys Gln Leu Ala Ser Lys Gly Ile Thr 
            20                  25                  30          


Val Val Leu Thr Ala Arg Asp Asp Lys Arg Gly Leu Glu Ala Val Glu 
        35                  40                  45              


Lys Leu Lys Glu Phe Asp Leu His Gly His Val Leu Phe His Gln Leu 
    50                  55                  60                  


Asp Val Ser Asp Thr Ala Ser Val Thr Ser Leu Ala Asp Phe Ile Lys 
65                  70                  75                  80  


Thr Gln Phe Gly Lys Leu Asp Ile Leu Val Asn Asn Ala Gly Ile Thr 
                85                  90                  95      


Gly Thr Thr Val Asp Ala Asp Ala Leu Ala Ser Ser Gly Tyr Gly Thr 
            100                 105                 110         


Gly Gly Glu Arg Lys Pro Ile Asp Trp Asn Lys Ile Val Ile Glu Thr 
        115                 120                 125             


Tyr Glu Ser Val Glu Lys Ala Ile Asn Thr Asn Tyr Tyr Gly Ala Lys 
    130                 135                 140                 


Arg Met Ala Glu Ala Leu Ile Pro Leu Leu Gln Val Ser Asp Ser Pro 
145                 150                 155                 160 


Arg Ile Val Asn Ala Ser Ser Pro Met Ala Lys Leu Glu Asn Ile Pro 
                165                 170                 175     


Ser Gly Trp Gly Lys Glu Val Leu Ser Asp Val Asp Ser Leu Thr Glu 
            180                 185                 190         


Glu Lys Leu Asp Glu Met Leu Thr Gln Leu Leu Lys Asp Phe Lys Glu 
        195                 200                 205             


Gly Ser Leu Glu Thr Lys Gly Trp Pro Thr Leu Met Ser Ser Tyr Ile 
    210                 215                 220                 


Ile Ser Lys Ala Ala Leu Asn Ala Tyr Thr Arg Ile Leu Ala Lys Lys 
225                 230                 235                 240 


Tyr Pro Ser Phe Cys Ile Asn Cys Val Asp Pro Gly His Val Lys Thr 
                245                 250                 255     


Asp Ile Asn Arg His Thr Gly His Leu Ser Ile Asp Glu Gly Ala Glu 
            260                 265                 270         


Ser His Val Arg Leu Ala Leu Leu Pro Asp Gly Gly Pro Ser Gly His 
        275                 280                 285             


Phe Phe Ser Arg Thr Glu Glu Thr Pro Phe 
    290                 295             


<210>  49
<211>  915
<212>  DNA
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  A nucleic acid molecule comprising a nucleotide which encodes the
       enzyme according to SEQ ID NO 50. QsFSL-2

<400>  49
atgggttcag atggaaggga tgtagcagag aggtatgcag tggttacagg tgcaaacaaa       60

ggcataggcc tagaaaccgt gcggcaacta gcgtctcacg gcattacagt tgtgttgaca      120

gctcgagatg agaagagagg gactgaagcc acaagaaagc tccaccagct gggtttgtca      180

aatttgattt tccatcagct ggatgtttta gaccctgtta gcattcagtc actggccaag      240

ttcatccaag acaaatttgg caggcttgat atcctggtta ataatgctgg agcatctgga      300

cttgcagctg atgagaaagc tctgaaggca ttaaacatag ataatgcagc ttggctctca      360

ggcaaggccg ccaatttagt tcaaggaatt gtcacacata cctatgagca aggcgaagaa      420

tgcataaata caaactatta tggtgtcaaa agggtgacgg aagctctcct accgctgtta      480

caactttccc ctataggagc aaggataata aatgtttcct cttgcagggg tgagctaaag      540

aggattccaa tgaacgtaag aaatgaactg ggcgacatca aagttctgac tgaaggcaga      600

atagatgcaa ttttgatgaa atttctacac gattttaagg ataatgcact tgagtccaac      660

ggatggacat tgatggggcc tgcttatagc atttcgaagg ccagtctcaa tgcctacact      720

agacttcttg ccaaaaagta ccccgagatg ctcattaact gtgttcatcc tggttatgtc      780

aacacagata tgacttggca tagagggata ctgacggtag aagagggtgc taaaggccca      840

gccatgctag ctcttttgca agatggagga cctacaggtt gctattttga tagtactcaa      900

caggcagaat tttaa                                                       915


<210>  50
<211>  304
<212>  PRT
<213>  Quillaja saponaria


<220>
<221>  misc_feature
<223>  An oxidoreductase enzyme capable of enhancing the activity of a 
       fucosyltransferase (QsFSL-2)

<400>  50

Met Gly Ser Asp Gly Arg Asp Val Ala Glu Arg Tyr Ala Val Val Thr 
1               5                   10                  15      


Gly Ala Asn Lys Gly Ile Gly Leu Glu Thr Val Arg Gln Leu Ala Ser 
            20                  25                  30          


His Gly Ile Thr Val Val Leu Thr Ala Arg Asp Glu Lys Arg Gly Thr 
        35                  40                  45              


Glu Ala Thr Arg Lys Leu His Gln Leu Gly Leu Ser Asn Leu Ile Phe 
    50                  55                  60                  


His Gln Leu Asp Val Leu Asp Pro Val Ser Ile Gln Ser Leu Ala Lys 
65                  70                  75                  80  


Phe Ile Gln Asp Lys Phe Gly Arg Leu Asp Ile Leu Val Asn Asn Ala 
                85                  90                  95      


Gly Ala Ser Gly Leu Ala Ala Asp Glu Lys Ala Leu Lys Ala Leu Asn 
            100                 105                 110         


Ile Asp Asn Ala Ala Trp Leu Ser Gly Lys Ala Ala Asn Leu Val Gln 
        115                 120                 125             


Gly Ile Val Thr His Thr Tyr Glu Gln Gly Glu Glu Cys Ile Asn Thr 
    130                 135                 140                 


Asn Tyr Tyr Gly Val Lys Arg Val Thr Glu Ala Leu Leu Pro Leu Leu 
145                 150                 155                 160 


Gln Leu Ser Pro Ile Gly Ala Arg Ile Ile Asn Val Ser Ser Cys Arg 
                165                 170                 175     


Gly Glu Leu Lys Arg Ile Pro Met Asn Val Arg Asn Glu Leu Gly Asp 
            180                 185                 190         


Ile Lys Val Leu Thr Glu Gly Arg Ile Asp Ala Ile Leu Met Lys Phe 
        195                 200                 205             


Leu His Asp Phe Lys Asp Asn Ala Leu Glu Ser Asn Gly Trp Thr Leu 
    210                 215                 220                 


Met Gly Pro Ala Tyr Ser Ile Ser Lys Ala Ser Leu Asn Ala Tyr Thr 
225                 230                 235                 240 


Arg Leu Leu Ala Lys Lys Tyr Pro Glu Met Leu Ile Asn Cys Val His 
                245                 250                 255     


Pro Gly Tyr Val Asn Thr Asp Met Thr Trp His Arg Gly Ile Leu Thr 
            260                 265                 270         


Val Glu Glu Gly Ala Lys Gly Pro Ala Met Leu Ala Leu Leu Gln Asp 
        275                 280                 285             


Gly Gly Pro Thr Gly Cys Tyr Phe Asp Ser Thr Gln Gln Ala Glu Phe 
    290                 295                 300                 


<210>  51
<211>  909
<212>  DNA
<213>  Salvia officinalis


<220>
<221>  misc_feature
<223>  A nucleic acid molecule comprising a nucleotide which encodes the
       enzyme according to SEQ ID NO 52. SoFSL-1

<400>  51
atggctgaag catcctcatt tcttgcacag aaaaggtatg cggtcgtgac aggagcaaac       60

aaaggactag gactagaaat atgcggacag cttgcttcac agggggtgac ggtactgctg      120

acatccagag atgaaaaacg aggcttagaa gccattgagg agcttaagaa atcggggatt      180

aattcggaaa atcttgaata tcatcagctg gatgttacta agccagctag tttcgcttct      240

ctggccgatt tcatcaaggc caaatttggc aagcttgata tcctggtgaa caatgcaggg      300

atcagcggtg ttattgtaga ttatgcagct ttaatggaag ccattcgccg tcgaggggca      360

gagatcaatt acgatggagt gatgaaacag acctacgagc tagcagagga atgcttgcaa      420

acaaattact atggtgtgaa aagaaccatt aatgctctcc ttccgctact tcagttttcc      480

gattcaccaa ggatcgtcaa tgtttcctcc gatgttggcc tccttaagaa aatacccggc      540

gagagaatca gagaagcctt aggcgacgtg gaaaaactta cggaagaaag cgtggacggg      600

attttagacg agtttctaag agatttcaag gaaggcaaga tcgcagagaa aggttggcct      660

acgtttaaga gcgcctattc aatctcaaag gcggcgctca attcgtacac gagggtttta      720

gcacggaaat acccgtcgat catcatcaac tgtgtctgcc cgggtgtcgt caaaaccgat      780

atcaatctta aaatgggcca cttgacggtt gaagaaggcg cggccagtcc cgtgaggtta      840

gcactcatgc cccttggttc gccttccggc ctgttctata ctcgaaacga agtaactcca      900

tttgaatga                                                              909


<210>  52
<211>  302
<212>  PRT
<213>  Salvia officinalis


<220>
<221>  misc_feature
<223>  An oxidoreductase enzyme capable of enhancing the activity of a 
       fucosyltransferase (SoFSL-1)

<400>  52

Met Ala Glu Ala Ser Ser Phe Leu Ala Gln Lys Arg Tyr Ala Val Val 
1               5                   10                  15      


Thr Gly Ala Asn Lys Gly Leu Gly Leu Glu Ile Cys Gly Gln Leu Ala 
            20                  25                  30          


Ser Gln Gly Val Thr Val Leu Leu Thr Ser Arg Asp Glu Lys Arg Gly 
        35                  40                  45              


Leu Glu Ala Ile Glu Glu Leu Lys Lys Ser Gly Ile Asn Ser Glu Asn 
    50                  55                  60                  


Leu Glu Tyr His Gln Leu Asp Val Thr Lys Pro Ala Ser Phe Ala Ser 
65                  70                  75                  80  


Leu Ala Asp Phe Ile Lys Ala Lys Phe Gly Lys Leu Asp Ile Leu Val 
                85                  90                  95      


Asn Asn Ala Gly Ile Ser Gly Val Ile Val Asp Tyr Ala Ala Leu Met 
            100                 105                 110         


Glu Ala Ile Arg Arg Arg Gly Ala Glu Ile Asn Tyr Asp Gly Val Met 
        115                 120                 125             


Lys Gln Thr Tyr Glu Leu Ala Glu Glu Cys Leu Gln Thr Asn Tyr Tyr 
    130                 135                 140                 


Gly Val Lys Arg Thr Ile Asn Ala Leu Leu Pro Leu Leu Gln Phe Ser 
145                 150                 155                 160 


Asp Ser Pro Arg Ile Val Asn Val Ser Ser Asp Val Gly Leu Leu Lys 
                165                 170                 175     


Lys Ile Pro Gly Glu Arg Ile Arg Glu Ala Leu Gly Asp Val Glu Lys 
            180                 185                 190         


Leu Thr Glu Glu Ser Val Asp Gly Ile Leu Asp Glu Phe Leu Arg Asp 
        195                 200                 205             


Phe Lys Glu Gly Lys Ile Ala Glu Lys Gly Trp Pro Thr Phe Lys Ser 
    210                 215                 220                 


Ala Tyr Ser Ile Ser Lys Ala Ala Leu Asn Ser Tyr Thr Arg Val Leu 
225                 230                 235                 240 


Ala Arg Lys Tyr Pro Ser Ile Ile Ile Asn Cys Val Cys Pro Gly Val 
                245                 250                 255     


Val Lys Thr Asp Ile Asn Leu Lys Met Gly His Leu Thr Val Glu Glu 
            260                 265                 270         


Gly Ala Ala Ser Pro Val Arg Leu Ala Leu Met Pro Leu Gly Ser Pro 
        275                 280                 285             


Ser Gly Leu Phe Tyr Thr Arg Asn Glu Val Thr Pro Phe Glu 
    290                 295                 300         


<210>  53
<211>  900
<212>  DNA
<213>  Spinacia oleracea


<220>
<221>  misc_feature
<223>  A nucleic acid molecule comprising a nucleotide which encodes the
       enzyme according to SEQ ID NO 54. SpolFSL

<400>  53
atggctgaac aatccaactt tctggctgaa aaaaggtatg cagtagtgac aggtgcaaac       60

aaaggaatag ggcttgaaat atgcagacag cttgcttctc aaggtgtgat tgtacttatc      120

acttctagag atggaaagaa aggattagaa gcccttaatg atctcattaa atctggaatt      180

agctctgata atcttcatta tcatcagctt gatgttactg accctatgag tattactgct      240

cttgctggtt tcatcaattc caaatttggc aagcttgata ttctggtgaa caatgctggg      300

ataggtggat ttataattga ctacgatgct atcaaagcaa taggttttcg caatatcaat      360

tatgacgaga tgatgacaca aacatatgag cttgcaaaag aatgcttgga aacaaactac      420

tatggagtta agagaacaac tgaagctttg cttcctcagc tggagttatc ggattcacca      480

aggatcatca atgtctcctc ttctacgggg atgttgaaga atataccaaa tgagaggatc      540

agaggagtct tgggtgatgc agagaatctt acagaagaaa aagttgaagc gattttgaat      600

gagttactga cagatttcaa agatggttca ttcaaagaga aagaatggcc ttctagaatg      660

gcagcttata cactgtcaaa ggcggctttg aatgcatatg caagaatatt ggctaagaaa      720

tacccgtcaa ttatcatcag ttgtgtttgt cctggtgtta ctaagacaga tatgaacgga      780

aacttgggac aattaacagt tgaagaaggg gccgcaagtc cggtgagagt agcattgatg      840

cctcatggtt caccttccgg tcttttctat gcaagaagcg aagtttcttc atatgaataa      900


<210>  54
<211>  299
<212>  PRT
<213>  Spinacia oleracea


<220>
<221>  misc_feature
<223>  An oxidoreductase enzyme capable of enhancing the activity of a 
       fucosyltransferase  (SpolFSL).

<400>  54

Met Ala Glu Gln Ser Asn Phe Leu Ala Glu Lys Arg Tyr Ala Val Val 
1               5                   10                  15      


Thr Gly Ala Asn Lys Gly Ile Gly Leu Glu Ile Cys Arg Gln Leu Ala 
            20                  25                  30          


Ser Gln Gly Val Ile Val Leu Ile Thr Ser Arg Asp Gly Lys Lys Gly 
        35                  40                  45              


Leu Glu Ala Leu Asn Asp Leu Ile Lys Ser Gly Ile Ser Ser Asp Asn 
    50                  55                  60                  


Leu His Tyr His Gln Leu Asp Val Thr Asp Pro Met Ser Ile Thr Ala 
65                  70                  75                  80  


Leu Ala Gly Phe Ile Asn Ser Lys Phe Gly Lys Leu Asp Ile Leu Val 
                85                  90                  95      


Asn Asn Ala Gly Ile Gly Gly Phe Ile Ile Asp Tyr Asp Ala Ile Lys 
            100                 105                 110         


Ala Ile Gly Phe Arg Asn Ile Asn Tyr Asp Glu Met Met Thr Gln Thr 
        115                 120                 125             


Tyr Glu Leu Ala Lys Glu Cys Leu Glu Thr Asn Tyr Tyr Gly Val Lys 
    130                 135                 140                 


Arg Thr Thr Glu Ala Leu Leu Pro Gln Leu Glu Leu Ser Asp Ser Pro 
145                 150                 155                 160 


Arg Ile Ile Asn Val Ser Ser Ser Thr Gly Met Leu Lys Asn Ile Pro 
                165                 170                 175     


Asn Glu Arg Ile Arg Gly Val Leu Gly Asp Ala Glu Asn Leu Thr Glu 
            180                 185                 190         


Glu Lys Val Glu Ala Ile Leu Asn Glu Leu Leu Thr Asp Phe Lys Asp 
        195                 200                 205             


Gly Ser Phe Lys Glu Lys Glu Trp Pro Ser Arg Met Ala Ala Tyr Thr 
    210                 215                 220                 


Leu Ser Lys Ala Ala Leu Asn Ala Tyr Ala Arg Ile Leu Ala Lys Lys 
225                 230                 235                 240 


Tyr Pro Ser Ile Ile Ile Ser Cys Val Cys Pro Gly Val Thr Lys Thr 
                245                 250                 255     


Asp Met Asn Gly Asn Leu Gly Gln Leu Thr Val Glu Glu Gly Ala Ala 
            260                 265                 270         


Ser Pro Val Arg Val Ala Leu Met Pro His Gly Ser Pro Ser Gly Leu 
        275                 280                 285             


Phe Tyr Ala Arg Ser Glu Val Ser Ser Tyr Glu 
    290                 295                 


<210>  55
<211>  31
<212>  DNA
<213>  Artificial sequence

<220>
<223>  attB1F site required for Gateway® cloning

<400>  55
ggggacaagt ttgtacaaaa aagcaggctt a                                      31


<210>  56
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for Qs-28-O-FucT

<400>  56
atggagaatg ggagagttta caaatcc                                           27


<210>  57
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  attB2R site required for Gateway® cloning

<400>  57
ggggaccact ttgtacaaga aagctgggta                                        30


<210>  58
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for Qs-28-O-FucT

<400>  58
tcaagttgtg attccagcaa tgaattc                                           27


<210>  59
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for Qs-28-O-RhaT

<400>  59
atggcaaaaa ctgataagca gcttc                                             25


<210>  60
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for Qs-28-O-RhaT

<400>  60
ttaaatttgg aaaggttccc ttttg                                             25


<210>  61
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for Qs-28-O-XylT3

<400>  61
atggctgctg cagctcc                                                      17


<210>  62
<211>  34
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for Qs-28-O-XylT3

<400>  62
ttaattcctc ttaagagacc tgtaattttt gaag                                   34


<210>  63
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for Qs-28-O-XylT4

<400>  63
atggactcca cccacttgc                                                    19


<210>  64
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for Qs-28-O-XylT4

<400>  64
tcaaattgtt tttgttttca gctt                                              24


<210>  65
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for Qs-28-O-ApiT4

<400>  65
atggactcca cccacttgca gcc                                               23


<210>  66
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for Qs-28-O-ApiT4

<400>  66
tcaaattgtt tttgttttca gcttcg                                            26


<210>  67
<211>  15
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for QsAXS1

<400>  67
atggcgtcgg cgtca                                                        15


<210>  68
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for QsAXS1

<400>  68
ctagctggca actggtttcg                                                   20


<210>  69
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for QsFucSyn

<400>  69
atggcagaag caacgcagag gtatg                                             25


<210>  70
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for QsFucSyn

<400>  70
tcaaaatggt gcttcttctg tcctg                                             25


<210>  71
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for QsFSL-1

<400>  71
atggcagaag caacagagag g                                                 21


<210>  72
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for QsFSL-1

<400>  72
tcaaaatggt gtctcttcag tcctg                                             25


<210>  73
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for QsFSL-2

<400>  73
atgggttcag atggaaggga tg                                                22


<210>  74
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for QsFSL-2

<400>  74
ttaaaattct gcctgttgag tactatc                                           27


<210>  75
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for SoFSL-1

<400>  75
atggctgaag catcctcatt tc                                                22


<210>  76
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for SoFSL-1

<400>  76
tcattcaaat ggagttactt cgtttcg                                           27


<210>  77
<211>  29
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for SOAP3

<400>  77
atgatagaaa tcgggtatat tgtaaaatg                                         29


<210>  78
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for SOAP3

<400>  78
ttagtccctg agcttatgta taatg                                             25


<210>  79
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for SOAP4

<400>  79
atgatttcaa agagcgcgag                                                   20


<210>  80
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for SOAP4

<400>  80
ttaaaatcga tgtaaaataa tgtgggc                                           27


<210>  81
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for SOAP5

<400>  81
atggcaactt ctcacattcg c                                                 21


<210>  82
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for SOAP5

<400>  82
ttataaccat cccttaacaa cagg                                              24


<210>  83
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for SOAP6

<400>  83
atgacgggaa aaggaagaac g                                                 21


<210>  84
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for SOAP6

<400>  84
ttaggaggac gcaagccagt taatg                                             25


<210>  85
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for SpolFSL

<400>  85
atggctgaac aatccaactt tc                                                22


<210>  86
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PCR primer sequence for SpolFSL

<400>  86
ttattcatat gaagaaactt cgcttc                                            26




