                         SEQUENCE LISTING

<110>  Charit - Universittsmedizin Berlin
 
<120>  REPROGRAMMING IMMUNE CELLS BY TARGETED INTEGRATION OF 
       ZETA-DEFICIENT CHIMERIC ANTIGEN RECEPTOR TRANSGENES

<130>  XII 2280/20WO

<150>  EP20216649
<151>  2020-12-22

<160>  65    

<170>  PatentIn version 3.5

<210>  1
<211>  508
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR fragment

<400>  1

Ala Gln Ser Phe Gly Leu Leu Asp Pro Lys Leu Cys Tyr Leu Leu Asp 
1               5                   10                  15      


Gly Ile Leu Phe Ile Tyr Arg Val Ile Leu Thr Ala Leu Phe Leu Arg 
            20                  25                  30          


Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
        35                  40                  45              


Pro Gly Pro Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala 
    50                  55                  60                  


Leu Leu Leu His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr 
65                  70                  75                  80  


Ser Ser Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg 
                85                  90                  95      


Ala Ser Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro 
            100                 105                 110         


Asp Gly Thr Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser 
        115                 120                 125             


Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser 
    130                 135                 140                 


Leu Thr Ile Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys 
145                 150                 155                 160 


Gln Gln Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu 
                165                 170                 175     


Glu Ile Thr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
            180                 185                 190         


Gly Ser Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro 
        195                 200                 205             


Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro 
    210                 215                 220                 


Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu 
225                 230                 235                 240 


Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala 
                245                 250                 255     


Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val 
            260                 265                 270         


Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr 
        275                 280                 285             


Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp 
    290                 295                 300                 


Gly Gln Gly Thr Ser Val Thr Val Ser Ser Glu Ser Lys Tyr Gly Pro 
305                 310                 315                 320 


Pro Cys Pro Pro Cys Pro Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
                325                 330                 335     


Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr 
            340                 345                 350         


Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
        355                 360                 365             


Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
    370                 375                 380                 


Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys 
385                 390                 395                 400 


Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
                405                 410                 415     


Ala Leu His Asn Ala Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
            420                 425                 430         


Lys Lys Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val Leu 
        435                 440                 445             


Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
    450                 455                 460                 


Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
465                 470                 475                 480 


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
                485                 490                 495     


Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys 
            500                 505             


<210>  2
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  2

Ala Gln Ser Phe Gly Leu Leu Asp Pro Lys Leu Cys Tyr Leu Leu Asp 
1               5                   10                  15      


Gly Ile Leu Phe Ile Tyr Arg Val Ile Leu Thr Ala Leu Phe Leu Arg 
            20                  25                  30          


<210>  3
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  3

Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  4
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  4

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  5
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  5

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
            100                 105         


<210>  6
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  6

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  7
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  7

Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr 
            20                  25                  30          


Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys 
    50                  55                  60                  


Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu 
65                  70                  75                  80  


Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala 
                85                  90                  95      


Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Ser Val Thr Val Ser Ser 
        115                 120 


<210>  8
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  8

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
1               5                   10          


<210>  9
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  9

Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp 
1               5                   10                  15      


Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe 
            20                  25                  30          


Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 
        35                  40                  45              


Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 
    50                  55                  60                  


Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly 
65                  70                  75                  80  


Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn Ala Tyr 
                85                  90                  95      


Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
            100                 105         


<210>  10
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  10

Lys Asp Pro Lys 
1               


<210>  11
<211>  27
<212>  PRT
<213>  Homo sapiens

<400>  11

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          


<210>  12
<211>  41
<212>  PRT
<213>  Homo sapiens

<400>  12

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  13
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  13

000      


<210>  14
<211>  617
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR fragment

<400>  14

Ala Gln Ser Phe Gly Leu Leu Asp Pro Lys Leu Cys Tyr Leu Leu Asp 
1               5                   10                  15      


Gly Ile Leu Phe Ile Tyr Arg Val Ile Leu Thr Ala Leu Phe Leu Arg 
            20                  25                  30          


Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
        35                  40                  45              


Pro Gly Pro Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala 
    50                  55                  60                  


Leu Leu Leu His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr 
65                  70                  75                  80  


Ser Ser Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg 
                85                  90                  95      


Ala Ser Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro 
            100                 105                 110         


Asp Gly Thr Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser 
        115                 120                 125             


Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser 
    130                 135                 140                 


Leu Thr Ile Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys 
145                 150                 155                 160 


Gln Gln Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu 
                165                 170                 175     


Glu Ile Thr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
            180                 185                 190         


Gly Ser Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro 
        195                 200                 205             


Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro 
    210                 215                 220                 


Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu 
225                 230                 235                 240 


Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala 
                245                 250                 255     


Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val 
            260                 265                 270         


Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr 
        275                 280                 285             


Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp 
    290                 295                 300                 


Gly Gln Gly Thr Ser Val Thr Val Ser Ser Glu Ser Lys Tyr Gly Pro 
305                 310                 315                 320 


Pro Cys Pro Pro Cys Pro Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
                325                 330                 335     


Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr 
            340                 345                 350         


Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
        355                 360                 365             


Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
    370                 375                 380                 


Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys 
385                 390                 395                 400 


Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
                405                 410                 415     


Ala Leu His Asn Ala Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
            420                 425                 430         


Lys Lys Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val Leu 
        435                 440                 445             


Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
    450                 455                 460                 


Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
465                 470                 475                 480 


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
                485                 490                 495     


Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser 
            500                 505                 510         


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
        515                 520                 525             


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
    530                 535                 540                 


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
545                 550                 555                 560 


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
                565                 570                 575     


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
            580                 585                 590         


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
        595                 600                 605             


Leu His Met Gln Ala Leu Pro Pro Arg 
    610                 615         


<210>  15
<211>  505
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR fragment

<400>  15

Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Ala Thr Asn Phe Ser Leu 
1               5                   10                  15      


Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Ala Leu 
            20                  25                  30          


Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu His Ala Ala 
        35                  40                  45              


Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser 
    50                  55                  60                  


Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser 
65                  70                  75                  80  


Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu 
                85                  90                  95      


Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe 
            100                 105                 110         


Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu 
        115                 120                 125             


Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu 
    130                 135                 140                 


Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Gly Gly 
145                 150                 155                 160 


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Lys Leu 
                165                 170                 175     


Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Val 
            180                 185                 190         


Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp 
        195                 200                 205             


Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile Trp 
    210                 215                 220                 


Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr 
225                 230                 235                 240 


Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser 
                245                 250                 255     


Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr 
            260                 265                 270         


Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val 
        275                 280                 285             


Thr Val Ser Ser Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
    290                 295                 300                 


Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp 
305                 310                 315                 320 


Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe 
                325                 330                 335     


Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 
            340                 345                 350         


Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 
        355                 360                 365             


Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly 
    370                 375                 380                 


Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn Ala Tyr 
385                 390                 395                 400 


Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Lys Asp Pro Lys Phe 
                405                 410                 415     


Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu 
            420                 425                 430         


Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg 
        435                 440                 445             


Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro 
    450                 455                 460                 


Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala 
465                 470                 475                 480 


Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
                485                 490                 495     


Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
            500                 505 


<210>  16
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  16

Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
1               5                   10  


<210>  17
<211>  473
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  17

Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala 
            20                  25                  30          


Leu Leu Leu His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr 
        35                  40                  45              


Ser Ser Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg 
    50                  55                  60                  


Ala Ser Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro 
65                  70                  75                  80  


Asp Gly Thr Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser 
                85                  90                  95      


Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser 
            100                 105                 110         


Leu Thr Ile Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys 
        115                 120                 125             


Gln Gln Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu 
    130                 135                 140                 


Glu Ile Thr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
145                 150                 155                 160 


Gly Ser Glu Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro 
                165                 170                 175     


Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro 
            180                 185                 190         


Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu 
        195                 200                 205             


Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala 
    210                 215                 220                 


Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val 
225                 230                 235                 240 


Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr 
                245                 250                 255     


Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp 
            260                 265                 270         


Gly Gln Gly Thr Ser Val Thr Val Ser Ser Glu Ser Lys Tyr Gly Pro 
        275                 280                 285             


Pro Cys Pro Pro Cys Pro Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
    290                 295                 300                 


Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr 
305                 310                 315                 320 


Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
                325                 330                 335     


Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
            340                 345                 350         


Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys 
        355                 360                 365             


Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
    370                 375                 380                 


Ala Leu His Asn Ala Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
385                 390                 395                 400 


Lys Lys Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val Leu 
                405                 410                 415     


Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            420                 425                 430         


Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
        435                 440                 445             


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
    450                 455                 460                 


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
465                 470             


<210>  18
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  18

Arg Val Lys Phe Ser Arg Ser Ala 
1               5               


<210>  19
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  19

Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
1               5                   10                  


<210>  20
<211>  595
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR fragment

<400>  20

Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Ala Thr Asn Phe Ser Leu 
1               5                   10                  15      


Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Ala Leu 
            20                  25                  30          


Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu His Ala Ala 
        35                  40                  45              


Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser 
    50                  55                  60                  


Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser 
65                  70                  75                  80  


Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu 
                85                  90                  95      


Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe 
            100                 105                 110         


Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu 
        115                 120                 125             


Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu 
    130                 135                 140                 


Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Gly Gly 
145                 150                 155                 160 


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Lys Leu 
                165                 170                 175     


Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Val 
            180                 185                 190         


Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp 
        195                 200                 205             


Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile Trp 
    210                 215                 220                 


Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr 
225                 230                 235                 240 


Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser 
                245                 250                 255     


Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr 
            260                 265                 270         


Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val 
        275                 280                 285             


Thr Val Ser Ser Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
    290                 295                 300                 


Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp 
305                 310                 315                 320 


Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe 
                325                 330                 335     


Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 
            340                 345                 350         


Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 
        355                 360                 365             


Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly 
    370                 375                 380                 


Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn Ala Tyr 
385                 390                 395                 400 


Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Lys Asp Pro Lys Phe 
                405                 410                 415     


Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu 
            420                 425                 430         


Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg 
        435                 440                 445             


Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro 
    450                 455                 460                 


Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala 
465                 470                 475                 480 


Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
                485                 490                 495     


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
            500                 505                 510         


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
        515                 520                 525             


Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
    530                 535                 540                 


Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
545                 550                 555                 560 


Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
                565                 570                 575     


Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
            580                 585                 590         


Pro Pro Arg 
        595 


<210>  21
<211>  112
<212>  PRT
<213>  Homo sapiens

<400>  21

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  22
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  enhanced GFP

<400>  22

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 


<210>  23
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  23

Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys Ser 
1               5                   10                  15      


Ser Asp Lys Ser Val Cys Leu Phe Thr 
            20                  25  


<210>  24
<211>  2221
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR fragment

<400>  24
ggcaggagct ctgtgcacag agaacagcct catctgctcg ccttgtttcc acctcccctc       60

ccattgcccc aggttctttg gccccacagc ggccacatct gccgttggtg ccaataggtt      120

ttccaggagc tggttgaggt gggagggagg gagagggttg tgatcaggct gaggcatggg      180

gattggatat agtctccgtg tcatgattta tttggtcagt cagtcctagt gccaccctgg      240

ggtaatgggg atgtgttctc gtcaccttgg gcctggctga ccagctttat ctcttggcac      300

agaggcacag agctttggcc tgctggatcc caaactctgc tacctgctgg atggaatcct      360

cttcatctat cgtgtcattc tcactgcctt gttcctgaga gccaccaact tctctctttt      420

gaagcaggcc ggagatgtgg aagaaaatcc tgggcccatg gctcttcctg tgactgccct      480

tctgctgccc ctggctctcc tgctccatgc tgcccggcca gacatccaga tgacacagac      540

aaccagcagc ctcagcgcca gcctggggga cagagtgacc attagctgcc gggcctctca      600

ggacatcagc aaatacctga actggtacca gcagaaacca gatggcactg tcaagctgct      660

gatttaccac acctccaggc tccacagcgg cgtgcccagt cgcttcagcg gcagtgggag      720

cgggacagat tattccctca caatctccaa cctggagcag gaagatattg ccacatactt      780

ctgccagcaa ggcaacaccc tgccatacac atttggaggc ggcaccaaat tggagatcac      840

cggcggtggt ggatctggag gagggggcag cggaggtggc ggctctgagg tgaaactgca      900

ggagagtggc cctggcctgg tggctcccag ccagagcctt tctgtcacct gcaccgtgtc      960

tggggtgtcc ctgcctgact atggagtctc ctggatccgg cagcctccaa gaaaaggact     1020

ggaatggctg ggcgtcatct ggggaagtga gaccacctac tataattcag ccctcaagtc     1080

ccggctcacc atcattaagg acaactccaa atcccaggtg ttcctgaaga tgaattctct     1140

ccagactgat gacacagcca tctactactg tgccaagcat tattattacg gcgggtccta     1200

tgccatggac tactggggcc aggggaccag tgtcactgtt tcttctgaaa gcaagtatgg     1260

ccccccatgc cctccctgcc ccggacagcc gcgggagccc caggtctaca ccctcccacc     1320

ctccagagat gagctgacca agaaccaggt ttcactgaca tgcctggtga agggcttcta     1380

cccctctgac attgctgtgg agtgggagag caatgggcag ccagagaaca actacaagac     1440

cacacctcct gtcctggaca gcgacggctc cttcttcctc tacagtaagc tcactgtgga     1500

caagagccgc tggcagcagg gcaatgtctt ctcctgcagc gtgatgcacg aggccctgca     1560

caatgcctat acccagaagt cactctctct gagccctgga aagaaggatc ccaagttttg     1620

ggtcctcgtg gtggtcgggg gtgtgctggc ctgctacagc ttgctggtca cagtggcctt     1680

catcatcttc tgggtgcgct ccaagaggag ccggctgctt cacagtgatt acatgaacat     1740

gacccccagg aggccaggac ccaccaggaa gcactaccag ccctacgctc ccccgcggga     1800

ctttgctgct taccgcagca gagtgaaggt gggtaccact gggctttggg aggagggcac     1860

ggggtccccc acttgatgga tgttcagagg ggccttggtc ttggaaggtc tcaagctcgg     1920

gtggtgcctg gggcttggta tccaggagca aagcaaggac cagccaagtg tgtgccttga     1980

gtgggctgag gaggaggtgg cagtgtctgg ctgagatgga cagggtagga gggagagcct     2040

ggtgctaggc acctccatga caagccgtac aaatgtgtgc acatcagagt gtcccaggga     2100

aggcgatgct actggtgaca aaggggctta cactcaggca gaggtccttc tttccaagtg     2160

tgaatgaagg ccatgttagc ctttctcttg aaaaggccct ttcctcatct gtaactgggg     2220

a                                                                     2221


<210>  25
<211>  400
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR construct

<400>  25
ggcaggagct ctgtgcacag agaacagcct catctgctcg ccttgtttcc acctcccctc       60

ccattgcccc aggttctttg gccccacagc ggccacatct gccgttggtg ccaataggtt      120

ttccaggagc tggttgaggt gggagggagg gagagggttg tgatcaggct gaggcatggg      180

gattggatat agtctccgtg tcatgattta tttggtcagt cagtcctagt gccaccctgg      240

ggtaatgggg atgtgttctc gtcaccttgg gcctggctga ccagctttat ctcttggcac      300

agaggcacag agctttggcc tgctggatcc caaactctgc tacctgctgg atggaatcct      360

cttcatctat cgtgtcattc tcactgcctt gttcctgaga                            400


<210>  26
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  26
gccaccaact tctctctttt gaagcaggcc ggagatgtgg aagaaaatcc tgggccc          57


<210>  27
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  27
atggctcttc ctgtgactgc ccttctgctg cccctggctc tcctgctcca tgctgcccgg       60

cca                                                                     63


<210>  28
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  28
gacatccaga tgacacagac aaccagcagc ctcagcgcca gcctggggga cagagtgacc       60

attagctgcc gggcctctca ggacatcagc aaatacctga actggtacca gcagaaacca      120

gatggcactg tcaagctgct gatttaccac acctccaggc tccacagcgg cgtgcccagt      180

cgcttcagcg gcagtgggag cgggacagat tattccctca caatctccaa cctggagcag      240

gaagatattg ccacatactt ctgccagcaa ggcaacaccc tgccatacac atttggaggc      300

ggcaccaaat tggagatcac c                                                321


<210>  29
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  29
ggcggtggtg gatctggagg agggggcagc ggaggtggcg gctct                       45


<210>  30
<211>  360
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  30
gaggtgaaac tgcaggagag tggccctggc ctggtggctc ccagccagag cctttctgtc       60

acctgcaccg tgtctggggt gtccctgcct gactatggag tctcctggat ccggcagcct      120

ccaagaaaag gactggaatg gctgggcgtc atctggggaa gtgagaccac ctactataat      180

tcagccctca agtcccggct caccatcatt aaggacaact ccaaatccca ggtgttcctg      240

aagatgaatt ctctccagac tgatgacaca gccatctact actgtgccaa gcattattat      300

tacggcgggt cctatgccat ggactactgg ggccagggga ccagtgtcac tgtttcttct      360


<210>  31
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  31
gaaagcaagt atggcccccc atgccctccc tgcccc                                 36


<210>  32
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  32
ggacagccgc gggagcccca ggtctacacc ctcccaccct ccagagatga gctgaccaag       60

aaccaggttt cactgacatg cctggtgaag ggcttctacc cctctgacat tgctgtggag      120

tgggagagca atgggcagcc agagaacaac tacaagacca cacctcctgt cctggacagc      180

gacggctcct tcttcctcta cagtaagctc actgtggaca agagccgctg gcagcagggc      240

aatgtcttct cctgcagcgt gatgcacgag gccctgcaca atgcctatac ccagaagtca      300

ctctctctga gccctggaaa g                                                321


<210>  33
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  33
aaggatccca ag                                                           12


<210>  34
<211>  81
<212>  DNA
<213>  Homo sapiens

<400>  34
ttttgggtcc tcgtggtggt cgggggtgtg ctggcctgct acagcttgct ggtcacagtg       60

gccttcatca tcttctgggt g                                                 81


<210>  35
<211>  123
<212>  DNA
<213>  Homo sapiens

<400>  35
cgctccaaga ggagccggct gcttcacagt gattacatga acatgacccc caggaggcca       60

ggacccacca ggaagcacta ccagccctac gctcccccgc gggactttgc tgcttaccgc      120

agc                                                                    123


<210>  36
<211>  402
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR construct

<400>  36
agagtgaagg tgggtaccac tgggctttgg gaggagggca cggggtcccc cacttgatgg       60

atgttcagag gggccttggt cttggaaggt ctcaagctcg ggtggtgcct ggggcttggt      120

atccaggagc aaagcaagga ccagccaagt gtgtgccttg agtgggctga ggaggaggtg      180

gcagtgtctg gctgagatgg acagggtagg agggagagcc tggtgctagg cacctccatg      240

acaagccgta caaatgtgtg cacatcagag tgtcccaggg aaggcgatgc tactggtgac      300

aaaggggctt acactcaggc agaggtcctt ctttccaagt gtgaatgaag gccatgttag      360

cctttctctt gaaaaggccc tttcctcatc tgtaactggg ga                         402


<210>  37
<211>  2812
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR fragment

<400>  37
ggcaggagct ctgtgcacag agaacagcct catctgctcg ccttgtttcc acctcccctc       60

ccattgcccc aggttctttg gccccacagc ggccacatct gccgttggtg ccaataggtt      120

ttccaggagc tggttgaggt gggagggagg gagagggttg tgatcaggct gaggcatggg      180

gattggatat agtctccgtg tcatgattta tttggtcagt cagtcctagt gccaccctgg      240

ggtaatgggg atgtgttctc gtcaccttgg gcctggctga ccagctttat ctcttggcac      300

agaggcacag agctttggcc tgctggatcc caaactctgc tacctgctgg atggaatcct      360

cttcatctat cgtgtcattc tcactgcctt gttcctgaga gccaccaact tctctctttt      420

gaagcaggcc ggagatgtgg aagaaaatcc tgggcccatg gctcttcctg tgactgccct      480

tctgctgccc ctggctctcc tgctccatgc tgcccggcca gacatccaga tgacacagac      540

aaccagcagc ctcagcgcca gcctggggga cagagtgacc attagctgcc gggcctctca      600

ggacatcagc aaatacctga actggtacca gcagaaacca gatggcactg tcaagctgct      660

gatttaccac acctccaggc tccacagcgg cgtgcccagt cgcttcagcg gcagtgggag      720

cgggacagat tattccctca caatctccaa cctggagcag gaagatattg ccacatactt      780

ctgccagcaa ggcaacaccc tgccatacac atttggaggc ggcaccaaat tggagatcac      840

cggcggtggt ggatctggag gagggggcag cggaggtggc ggctctgagg tgaaactgca      900

ggagagtggc cctggcctgg tggctcccag ccagagcctt tctgtcacct gcaccgtgtc      960

tggggtgtcc ctgcctgact atggagtctc ctggatccgg cagcctccaa gaaaaggact     1020

ggaatggctg ggcgtcatct ggggaagtga gaccacctac tataattcag ccctcaagtc     1080

ccggctcacc atcattaagg acaactccaa atcccaggtg ttcctgaaga tgaattctct     1140

ccagactgat gacacagcca tctactactg tgccaagcat tattattacg gcgggtccta     1200

tgccatggac tactggggcc aggggaccag tgtcactgtt tcttctgaaa gcaagtatgg     1260

ccccccatgc cctccctgcc ccggacagcc gcgggagccc caggtctaca ccctcccacc     1320

ctccagagat gagctgacca agaaccaggt ttcactgaca tgcctggtga agggcttcta     1380

cccctctgac attgctgtgg agtgggagag caatgggcag ccagagaaca actacaagac     1440

cacacctcct gtcctggaca gcgacggctc cttcttcctc tacagtaagc tcactgtgga     1500

caagagccgc tggcagcagg gcaatgtctt ctcctgcagc gtgatgcacg aggccctgca     1560

caatgcctat acccagaagt cactctctct gagccctgga aagaaggatc ccaagttttg     1620

ggtcctcgtg gtggtcgggg gtgtgctggc ctgctacagc ttgctggtca cagtggcctt     1680

catcatcttc tgggtgcgct ccaagaggag ccggctgctt cacagtgatt acatgaacat     1740

gacccccagg aggccaggac ccaccaggaa gcactaccag ccctacgctc ccccgcggga     1800

ctttgctgct taccgcagca gggtcaaatt ttctagatct gcagatgcgc cggcctatca     1860

acaaggccag aaccagctct ataacgagct caatctagga cgaagagagg agtacgatgt     1920

tttggacaag agacgtggcc gggaccctga gatgggggga aagccgagaa ggaagaaccc     1980

tcaggaaggc ctgtacaatg aactgcagaa agataagatg gcggaggcct acagtgagat     2040

tgggatgaaa ggcgagcgcc ggaggggcaa ggggcacgat ggcctttacc agggtctcag     2100

tacagccacc aaggacacct acgacgccct tcacatgcag gccctgcccc ctcgctaata     2160

agaattctaa ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct     2220

gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt     2280

tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg     2340

ggtggggtgg ggcaggacag caagggggag gattgggaag agaatagcag gcatgctggg     2400

gagcggccgc agagtgaagg tgggtaccac tgggctttgg gaggagggca cggggtcccc     2460

cacttgatgg atgttcagag gggccttggt cttggaaggt ctcaagctcg ggtggtgcct     2520

ggggcttggt atccaggagc aaagcaagga ccagccaagt gtgtgccttg agtgggctga     2580

ggaggaggtg gcagtgtctg gctgagatgg acagggtagg agggagagcc tggtgctagg     2640

cacctccatg acaagccgta caaatgtgtg cacatcagag tgtcccaggg aaggcgatgc     2700

tactggtgac aaaggggctt acactcaggc agaggtcctt ctttccaagt gtgaatgaag     2760

gccatgttag cctttctctt gaaaaggccc tttcctcatc tgtaactggg ga             2812


<210>  38
<211>  2258
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR fragment

<400>  38
attgtcctca gagtccccgg aggaagggat cctagagaat actcatgtcc ccgggggcgc       60

agaccaagaa ccttatacct ctgcgcacgc gcgagggcgc tagcccggga agaataaact      120

ccagcgggtc ctgggccagc gtgatgaatg cacgtgtcac aggcgggagg aaaaaggaca      180

actgggctca gaaaaagggg ctgccacgcc ccggcccctg caagggctca gccccaggag      240

gcagggcagc attgttagtt gccaaggagc ggagtagggc tgtccctcgg gtcgccgcgc      300

gccctggcac cccggctccc ctgcgcgccc tcgcggagca gtaacgcgct ttgctttctg      360

tgttgcagtt cagcaggagc gcagacgccc ccgcgtacgc caccaacttc tctcttttga      420

agcaggccgg agatgtggaa gaaaatcctg ggcccatggc tcttcctgtg actgcccttc      480

tgctgcccct ggctctcctg ctccatgctg cccggccaga catccagatg acacagacaa      540

ccagcagcct cagcgccagc ctgggggaca gagtgaccat tagctgccgg gcctctcagg      600

acatcagcaa atacctgaac tggtaccagc agaaaccaga tggcactgtc aagctgctga      660

tttaccacac ctccaggctc cacagcggcg tgcccagtcg cttcagcggc agtgggagcg      720

ggacagatta ttccctcaca atctccaacc tggagcagga agatattgcc acatacttct      780

gccagcaagg caacaccctg ccatacacat ttggaggcgg caccaaattg gagatcaccg      840

gcggtggtgg atctggagga gggggcagcg gaggtggcgg ctctgaggtg aaactgcagg      900

agagtggccc tggcctggtg gctcccagcc agagcctttc tgtcacctgc accgtgtctg      960

gggtgtccct gcctgactat ggagtctcct ggatccggca gcctccaaga aaaggactgg     1020

aatggctggg cgtcatctgg ggaagtgaga ccacctacta taattcagcc ctcaagtccc     1080

ggctcaccat cattaaggac aactccaaat cccaggtgtt cctgaagatg aattctctcc     1140

agactgatga cacagccatc tactactgtg ccaagcatta ttattacggc gggtcctatg     1200

ccatggacta ctggggccag gggaccagtg tcactgtttc ttctgaaagc aagtatggcc     1260

ccccatgccc tccctgcccc ggacagccgc gggagcccca ggtctacacc ctcccaccct     1320

ccagagatga gctgaccaag aaccaggttt cactgacatg cctggtgaag ggcttctacc     1380

cctctgacat tgctgtggag tgggagagca atgggcagcc agagaacaac tacaagacca     1440

cacctcctgt cctggacagc gacggctcct tcttcctcta cagtaagctc actgtggaca     1500

agagccgctg gcagcagggc aatgtcttct cctgcagcgt gatgcacgag gccctgcaca     1560

atgcctatac ccagaagtca ctctctctga gccctggaaa gaaggatccc aagttttggg     1620

tcctcgtggt ggtcgggggt gtgctggcct gctacagctt gctggtcaca gtggccttca     1680

tcatcttctg ggtgcgctcc aagaggagcc ggctgcttca cagtgattac atgaacatga     1740

cccccaggag gccaggaccc accaggaagc actaccagcc ctacgctccc ccgcgggact     1800

ttgctgctta ccgcagcagg gtcaaatttt ctagatctgc agatgcgccg gcctatcaac     1860

aaggccagaa ccagctctat aacgtaagtc agcctcgccg tagaccctcc ggaaaggaag     1920

ggcagcctcc tcctcggacc tagggaacgc gcccgccaga gtcttgggtt taggtttggt     1980

ccctagctat cacctctgcg acccggctgc aacttgttta acctcagttt tctaacccac     2040

aaaacaggtg tagtaattgt acctgtctca aagggcgcca taaagattac atgctttaat     2100

gtcttgatgc atggaacgag cttagtctag tgcctgccac tagtgagccc ccaagccatc     2160

agcaatttgc aaaggctaga ggggggtgga gaaggaatgt attagaatct tcccagagga     2220

ggggaaagcg atttcacact ccctgtgcaa tgggactg                             2258


<210>  39
<211>  398
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR construct

<400>  39
attgtcctca gagtccccgg aggaagggat cctagagaat actcatgtcc ccgggggcgc       60

agaccaagaa ccttatacct ctgcgcacgc gcgagggcgc tagcccggga agaataaact      120

ccagcgggtc ctgggccagc gtgatgaatg cacgtgtcac aggcgggagg aaaaaggaca      180

actgggctca gaaaaagggg ctgccacgcc ccggcccctg caagggctca gccccaggag      240

gcagggcagc attgttagtt gccaaggagc ggagtagggc tgtccctcgg gtcgccgcgc      300

gccctggcac cccggctccc ctgcgcgccc tcgcggagca gtaacgcgct ttgctttctg      360

tgttgcagtt cagcaggagc gcagacgccc ccgcgtac                              398


<210>  40
<211>  1419
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR body

<400>  40
gccaccaact tctctctttt gaagcaggcc ggagatgtgg aagaaaatcc tgggcccatg       60

gctcttcctg tgactgccct tctgctgccc ctggctctcc tgctccatgc tgcccggcca      120

gacatccaga tgacacagac aaccagcagc ctcagcgcca gcctggggga cagagtgacc      180

attagctgcc gggcctctca ggacatcagc aaatacctga actggtacca gcagaaacca      240

gatggcactg tcaagctgct gatttaccac acctccaggc tccacagcgg cgtgcccagt      300

cgcttcagcg gcagtgggag cgggacagat tattccctca caatctccaa cctggagcag      360

gaagatattg ccacatactt ctgccagcaa ggcaacaccc tgccatacac atttggaggc      420

ggcaccaaat tggagatcac cggcggtggt ggatctggag gagggggcag cggaggtggc      480

ggctctgagg tgaaactgca ggagagtggc cctggcctgg tggctcccag ccagagcctt      540

tctgtcacct gcaccgtgtc tggggtgtcc ctgcctgact atggagtctc ctggatccgg      600

cagcctccaa gaaaaggact ggaatggctg ggcgtcatct ggggaagtga gaccacctac      660

tataattcag ccctcaagtc ccggctcacc atcattaagg acaactccaa atcccaggtg      720

ttcctgaaga tgaattctct ccagactgat gacacagcca tctactactg tgccaagcat      780

tattattacg gcgggtccta tgccatggac tactggggcc aggggaccag tgtcactgtt      840

tcttctgaaa gcaagtatgg ccccccatgc cctccctgcc ccggacagcc gcgggagccc      900

caggtctaca ccctcccacc ctccagagat gagctgacca agaaccaggt ttcactgaca      960

tgcctggtga agggcttcta cccctctgac attgctgtgg agtgggagag caatgggcag     1020

ccagagaaca actacaagac cacacctcct gtcctggaca gcgacggctc cttcttcctc     1080

tacagtaagc tcactgtgga caagagccgc tggcagcagg gcaatgtctt ctcctgcagc     1140

gtgatgcacg aggccctgca caatgcctat acccagaagt cactctctct gagccctgga     1200

aagaaggatc ccaagttttg ggtcctcgtg gtggtcgggg gtgtgctggc ctgctacagc     1260

ttgctggtca cagtggcctt catcatcttc tgggtgcgct ccaagaggag ccggctgctt     1320

cacagtgatt acatgaacat gacccccagg aggccaggac ccaccaggaa gcactaccag     1380

ccctacgctc ccccgcggga ctttgctgct taccgcagc                            1419


<210>  41
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR component

<400>  41
agggtcaaat tttctagatc tgca                                              24


<210>  42
<211>  417
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR construct

<400>  42
gatgcgccgg cctatcaaca aggccagaac cagctctata acgtaagtca gcctcgccgt       60

agaccctccg gaaaggaagg gcagcctcct cctcggacct agggaacgcg cccgccagag      120

tcttgggttt aggtttggtc cctagctatc acctctgcga cccggctgca acttgtttaa      180

cctcagtttt ctaacccaca aaacaggtgt agtaattgta cctgtctcaa agggcgccat      240

aaagattaca tgctttaatg tcttgatgca tggaacgagc ttagtctagt gcctgccact      300

agtgagcccc caagccatca gcaatttgca aaggctagag gggggtggag aaggaatgta      360

ttagaatctt cccagaggag gggaaagcga tttcacactc cctgtgcaat gggactg         417


<210>  43
<211>  399
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR construct

<400>  43
caaggccaga accagctcta taacgtaagt cagcctcgcc gtagaccctc cggaaaggaa       60

gggcagcctc ctcctcggac ctagggaacg cgcccgccag agtcttgggt ttaggtttgg      120

tccctagcta tcacctctgc gacccggctg caacttgttt aacctcagtt ttctaaccca      180

caaaacaggt gtagtaattg tacctgtctc aaagggcgcc ataaagatta catgctttaa      240

tgtcttgatg catggaacga gcttagtcta gtgcctgcca ctagtgagcc cccaagccat      300

cagcaatttg caaaggctag aggggggtgg agaaggaatg tattagaatc ttcccagagg      360

aggggaaagc gatttcacac tccctgtgca atgggactg                             399


<210>  44
<211>  339
<212>  DNA
<213>  Homo sapiens

<400>  44
agggtcaaat tttctagatc tgcagatgcg ccggcctatc aacaaggcca gaaccagctc       60

tataacgagc tcaatctagg acgaagagag gagtacgatg ttttggacaa gagacgtggc      120

cgggaccctg agatgggggg aaagccgaga aggaagaacc ctcaggaagg cctgtacaat      180

gaactgcaga aagataagat ggcggaggcc tacagtgaga ttgggatgaa aggcgagcgc      240

cggaggggca aggggcacga tggcctttac cagggtctca gtacagccac caaggacacc      300

tacgacgccc ttcacatgca ggccctgccc cctcgctaa                             339


<210>  45
<211>  252
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR construct

<400>  45
taagaattct aactagagct cgctgatcag cctcgactgt gccttctagt tgccagccat       60

ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc      120

tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg      180

ggggtggggt ggggcaggac agcaaggggg aggattggga agagaatagc aggcatgctg      240

gggagcggcc gc                                                          252


<210>  46
<211>  720
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  enhanced GFP

<400>  46
atggtatcca agggtgaaga attgttcact ggcgtagttc ctatcttggt ggagctcgac       60

ggagatgtca atggacacaa gttttcagtc agtggcgaag gtgagggtga tgcaacttac      120

gggaagctta cactgaaatt catttgtact actggcaagt tgccggtgcc atggcccacg      180

ctggtaacga cccttaccta cggtgtccaa tgtttttctc gataccccga tcatatgaaa      240

cagcatgact tttttaaatc tgctatgccc gagggatacg tacaggaaag gactatattt      300

tttaaggacg atggtaacta taagacccgc gccgaagtca agtttgaggg agatactctt      360

gttaatcgaa tcgagctcaa ggggatagat tttaaggagg atggcaacat tctcggccac      420

aagctggagt acaattacaa tagtcacaat gtttacataa tggctgataa gcaaaaaaac      480

ggcatcaaag ttaattttaa aattaggcat aatatagaag acggttcagt tcaactcgcc      540

gaccactacc agcagaatac ccctattggc gatgggccgg ttctcctgcc tgacaaccat      600

tacctctcaa cacagtctgc tttgagtaaa gaccctaacg agaagcgaga tcatatggtc      660

ttgctcgagt tcgtaacggc cgccggtata accctgggca tggatgagct ctacaaataa      720


<210>  47
<211>  400
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR construct

<400>  47
cctattaaat aaaagaataa gcagtattat taagtagccc tgcatttcag gtttccttga       60

gtggcaggcc aggcctggcc gtgaacgttc actgaaatca tggcctcttg gccaagattg      120

atagcttgtg cctgtccctg agtcccagtc catcacgagc agctggtttc taagatgcta      180

tttcccgtat aaagcatgag accgtgactt gccagcccca cagagccccg cccttgtcca      240

tcactggcat ctggactcca gcctgggttg gggcaaagag ggaaatgaga tcatgtccta      300

accctgatcc tcttgtccca cagatatcca gaaccctgac cctgccgtgt accagctgag      360

agactctaaa tccagtgaca agtctgtctg cctattcacc                            400


<210>  48
<211>  399
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR construct

<400>  48
attttgattc tcaaacaaat gtgtcacaaa gtaaggattc tgatgtgtat atcacagaca       60

aaactgtgct agacatgagg tctatggact tcaagagcaa cagtgctgtg gcctggagca      120

acaaatctga ctttgcatgt gcaaacgcct tcaacaacag cattattcca gaagacacct      180

tcttccccag cccaggtaag ggcagctttg gtgccttcgc aggctgtttc cttgcttcag      240

gaatggccag gttctgccca gagctctggt caatgatgtc taaaactcct ctgattggtg      300

gtctcggcct tatccattgc caccaaaacc ctctttttac taagaaacag tgagccttgt      360

tctggcagtc cagagaatga cacgggaaaa aagcagatg                             399


<210>  49
<211>  399
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR construct

<400>  49
accgtttttc tggacaaccc caaagtaccc cgtctccctg gctttagcca cctctccatc       60

ctcttgcttt ctttgcctgg acaccccgtt ctcctgtgga ttcgggtcac ctctcactcc      120

tttcatttgg gcagctcccc tacccccctt acctctctag tctgtgctag ctcttccagc      180

cccctgtcat ggcatcttcc aggggtccga gagctcagct agtcttcttc ctccaacccg      240

ggcccctatg tccacttcag gacagcatgt ttgctgcctc cagggatcct gtgtccccga      300

gctgggacca ccttatattc ccagggccgg ttaatgtggc tctggttctg ggtactttta      360

tctgtcccct ccaccccaca gtggggccac tagggacag                             399


<210>  50
<211>  648
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter sequence

<400>  50
gtttattaca gggacagcag agatccagtt tggggatcaa ttgcatgaag aatctgctta       60

gggttaggcg ttttgcgctg cttcgcgagg atctgcgatc gctccggtgc ccgtcagtgg      120

gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc      180

ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc      240

ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt      300

tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct      360

tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg      420

cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg      480

tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc      540

acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc      600

cgttacagat ccaagctgtg accggcgcct acggctagcg ccgccacc                   648


<210>  51
<211>  400
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR construct

<400>  51
gattggtgac agaaaagccc catccttagg cctcctcctt cctagtctcc tgatattggg       60

tctaaccccc acctcctgtt aggcagattc cttatctggt gacacacccc catttcctgg      120

agccatctct ctccttgcca gaacctctaa ggtttgctta cgatggagcc agagaggatc      180

ctgggaggga gagcttggca gggggtggga gggaaggggg ggatgcgtga cctgcccggt      240

tctcagtggc caccctgcgc taccctctcc cagaacctga gctgctctga cgcggccgtc      300

tggtgcgttt cactgatcct ggtgctgcag cttccttaca cttcccaaga ggagaagcag      360

tttggaaaaa caaaatcaga ataagttggt cctgagttct                            400


<210>  52
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  guide RNA

<400>  52
gauggaaucc ucuucaucua                                                   20


<210>  53
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  guide RNA

<400>  53
gacgcccccg cguaccagca                                                   20


<210>  54
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  guide RNA

<400>  54
agagucucuc agcugguaca                                                   20


<210>  55
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  guide RNA

<400>  55
gggaaucaaa aucggugaau                                                   20


<210>  56
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  guide RNA

<400>  56
ggggccacua gggacaggau                                                   20


<210>  57
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  guide RNA

<400>  57
ugguacccac cuucacucuc                                                   20


<210>  58
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  guide RNA

<400>  58
ugccuuguuc cugagaguga                                                   20


<210>  59
<211>  434
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct

<400>  59

Ala Gln Ser Phe Gly Leu Leu Asp Pro Lys Leu Cys Tyr Leu Leu Asp 
1               5                   10                  15      


Gly Ile Leu Phe Ile Tyr Arg Val Ile Leu Thr Ala Leu Phe Leu Arg 
            20                  25                  30          


Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
        35                  40                  45              


Pro Gly Pro Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala 
    50                  55                  60                  


Leu Leu Leu His Ala Ala Arg Pro Glu Gln Lys Leu Ile Ser Glu Glu 
65                  70                  75                  80  


Asp Leu Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser 
                85                  90                  95      


Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser 
            100                 105                 110         


Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu 
        115                 120                 125             


Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe 
    130                 135                 140                 


Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu 
145                 150                 155                 160 


Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu 
                165                 170                 175     


Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Gly Gly 
            180                 185                 190         


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Lys Leu 
        195                 200                 205             


Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Val 
    210                 215                 220                 


Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp 
225                 230                 235                 240 


Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile Trp 
                245                 250                 255     


Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr 
            260                 265                 270         


Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser 
        275                 280                 285             


Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr 
    290                 295                 300                 


Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val 
305                 310                 315                 320 


Thr Val Ser Ser Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn 
                325                 330                 335     


Glu Lys Ser Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys 
            340                 345                 350         


Pro Ser Pro Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val 
        355                 360                 365             


Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala 
    370                 375                 380                 


Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser 
385                 390                 395                 400 


Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His 
                405                 410                 415     


Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg 
            420                 425                 430         


Val Lys 
        


<210>  60
<211>  1999
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR construct

<400>  60
ggcaggagct ctgtgcacag agaacagcct catctgctcg ccttgtttcc acctcccctc       60

ccattgcccc aggttctttg gccccacagc ggccacatct gccgttggtg ccaataggtt      120

ttccaggagc tggttgaggt gggagggagg gagagggttg tgatcaggct gaggcatggg      180

gattggatat agtctccgtg tcatgattta tttggtcagt cagtcctagt gccaccctgg      240

ggtaatgggg atgtgttctc gtcaccttgg gcctggctga ccagctttat ctcttggcac      300

agaggcacag agctttggcc tgctggatcc caaactctgc tacctgctgg atggaatcct      360

cttcatctat cgtgtcattc tcactgcctt gttcctgaga gccaccaact tctctctttt      420

gaagcaggcc ggagatgtgg aagaaaatcc tgggcccatg gctcttcctg tgactgccct      480

tctgctgccc ctggctctcc tgctccatgc tgcccggcca gagcaaaaac ttatctctga      540

agaggacctc gacatccaga tgacacagac aaccagcagc ctcagcgcca gcctggggga      600

cagagtgacc attagctgcc gggcctctca ggacatcagc aaatacctga actggtacca      660

gcagaaacca gatggcactg tcaagctgct gatttaccac acctccaggc tccacagcgg      720

cgtgcccagt cgcttcagcg gcagtgggag cgggacagat tattccctca caatctccaa      780

cctggagcag gaagatattg ccacatactt ctgccagcaa ggcaacaccc tgccatacac      840

atttggaggc ggcaccaaat tggagatcac cggcggtggt ggatctggag gagggggcag      900

cggaggtggc ggctctgagg tgaaactgca ggagagtggc cctggcctgg tggctcccag      960

ccagagcctt tctgtcacct gcaccgtgtc tggggtgtcc ctgcctgact atggagtctc     1020

ctggatccgg cagcctccaa gaaaaggact ggaatggctg ggcgtcatct ggggaagtga     1080

gaccacctac tataattcag ccctcaagtc ccggctcacc atcattaagg acaactccaa     1140

atcccaggtg ttcctgaaga tgaattctct ccagactgat gacacagcca tctactactg     1200

tgccaagcat tattattacg gcgggtccta tgccatggac tactggggcc aggggaccag     1260

tgtcactgtt tcttctatag aagtaatgta tccccctccc tacttggaca acgagaaatc     1320

taacggcaca atcatacacg ttaagggcaa gcatctgtgt ccctcccctc ttttccccgg     1380

accgtctaag ccattttggg tcctcgtggt ggtcgggggt gtgctggcct gctacagctt     1440

gctggtcaca gtggccttca tcatcttctg ggtgcgctcc aagaggagcc ggctgcttca     1500

cagtgattac atgaacatga cccccaggag gccaggaccc accaggaagc actaccagcc     1560

ctacgctccc ccgcgggact ttgctgctta ccgcagcaga gtgaaggtgg gtaccactgg     1620

gctttgggag gagggcacgg ggtcccccac ttgatggatg ttcagagggg ccttggtctt     1680

ggaaggtctc aagctcgggt ggtgcctggg gcttggtatc caggagcaaa gcaaggacca     1740

gccaagtgtg tgccttgagt gggctgagga ggaggtggca gtgtctggct gagatggaca     1800

gggtaggagg gagagcctgg tgctaggcac ctccatgaca agccgtacaa atgtgtgcac     1860

atcagagtgt cccagggaag gcgatgctac tggtgacaaa ggggcttaca ctcaggcaga     1920

ggtccttctt tccaagtgtg aatgaaggcc atgttagcct ttctcttgaa aaggcccttt     1980

cctcatctgt aactgggga                                                  1999


<210>  61
<211>  492
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct

<400>  61

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Asp 
            20                  25                  30          


Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly Asp 
        35                  40                  45              


Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr Leu 
    50                  55                  60                  


Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile Tyr 
65                  70                  75                  80  


His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 
                85                  90                  95      


Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln Glu 
            100                 105                 110         


Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr Thr 
        115                 120                 125             


Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu Ser 
145                 150                 155                 160 


Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys Thr 
                165                 170                 175     


Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln 
            180                 185                 190         


Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser Glu 
        195                 200                 205             


Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys 
    210                 215                 220                 


Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr 
225                 230                 235                 240 


Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly 
                245                 250                 255     


Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser 
            260                 265                 270         


Ser Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser 
        275                 280                 285             


Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro 
    290                 295                 300                 


Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly 
305                 310                 315                 320 


Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile 
                325                 330                 335     


Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met 
            340                 345                 350         


Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro 
        355                 360                 365             


Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe 
    370                 375                 380                 


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
385                 390                 395                 400 


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
                405                 410                 415     


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
            420                 425                 430         


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
        435                 440                 445             


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
    450                 455                 460                 


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
465                 470                 475                 480 


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490         


<210>  62
<211>  3175
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR construct

<400>  62
accgtttttc tggacaaccc caaagtaccc cgtctccctg gctttagcca cctctccatc       60

ctcttgcttt ctttgcctgg acaccccgtt ctcctgtgga ttcgggtcac ctctcactcc      120

tttcatttgg gcagctcccc tacccccctt acctctctag tctgtgctag ctcttccagc      180

cccctgtcat ggcatcttcc aggggtccga gagctcagct agtcttcttc ctccaacccg      240

ggcccctatg tccacttcag gacagcatgt ttgctgcctc cagggatcct gtgtccccga      300

gctgggacca ccttatattc ccagggccgg ttaatgtggc tctggttctg ggtactttta      360

tctgtcccct ccaccccaca gtggggccac tagggacagc attgattatt gagtagttat      420

taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca      480

taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca      540

ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg      600

gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg      660

ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc      720

ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg      780

atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca      840

agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt      900

ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg      960

gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg     1020

ccaccatggc tcttcctgtg actgcccttc tgctgcccct ggctctcctg ctccatgctg     1080

cccggccaga gcaaaaactt atctctgaag aggacctcga catccagatg acacagacaa     1140

ccagcagcct cagcgccagc ctgggggaca gagtgaccat tagctgccgg gcctctcagg     1200

acatcagcaa atacctgaac tggtaccagc agaaaccaga tggcactgtc aagctgctga     1260

tttaccacac ctccaggctc cacagcggcg tgcccagtcg cttcagcggc agtgggagcg     1320

ggacagatta ttccctcaca atctccaacc tggagcagga agatattgcc acatacttct     1380

gccagcaagg caacaccctg ccatacacat ttggaggcgg caccaaattg gagatcaccg     1440

gcggtggtgg atctggagga gggggcagcg gaggtggcgg ctctgaggtg aaactgcagg     1500

agagtggccc tggcctggtg gctcccagcc agagcctttc tgtcacctgc accgtgtctg     1560

gggtgtccct gcctgactat ggagtctcct ggatccggca gcctccaaga aaaggactgg     1620

aatggctggg cgtcatctgg ggaagtgaga ccacctacta taattcagcc ctcaagtccc     1680

ggctcaccat cattaaggac aactccaaat cccaggtgtt cctgaagatg aattctctcc     1740

agactgatga cacagccatc tactactgtg ccaagcatta ttattacggc gggtcctatg     1800

ccatggacta ctggggccag gggaccagtg tcactgtttc ttctatagaa gtaatgtatc     1860

cccctcccta cttggacaac gagaaatcta acggcacaat catacacgtt aagggcaagc     1920

atctgtgtcc ctcccctctt ttccccggac cgtctaagcc attttgggtc ctcgtggtgg     1980

tcgggggtgt gctggcctgc tacagcttgc tggtcacagt ggccttcatc atcttctggg     2040

tgcgctccaa gaggagccgg ctgcttcaca gtgattacat gaacatgacc cccaggaggc     2100

caggacccac caggaagcac taccagccct acgctccccc gcgggacttt gctgcttacc     2160

gcagcagggt caaattttct agatctgcag atgcgccggc ctatcaacaa ggccagaacc     2220

agctctataa cgagctcaat ctaggacgaa gagaggagta cgatgttttg gacaagagac     2280

gtggccggga ccctgagatg gggggaaagc cgagaaggaa gaaccctcag gaaggcctgt     2340

acaatgaact gcagaaagat aagatggcgg aggcctacag tgagattggg atgaaaggcg     2400

agcgccggag gggcaagggg cacgatggcc tttaccaggg tctcagtaca gccaccaagg     2460

acacctacga cgcccttcac atgcaggccc tgccccctcg ctaaagatct cgagtctagc     2520

tcgagggcgc gccccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg     2580

tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct     2640

aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg     2700

gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg     2760

cggtgggctc tatgggattg gtgacagaaa agccccatcc ttaggcctcc tccttcctag     2820

tctcctgata ttgggtctaa cccccacctc ctgttaggca gattccttat ctggtgacac     2880

acccccattt cctggagcca tctctctcct tgccagaacc tctaaggttt gcttacgatg     2940

gagccagaga ggatcctggg agggagagct tggcaggggg tgggagggaa gggggggatg     3000

cgtgacctgc ccggttctca gtggccaccc tgcgctaccc tctcccagaa cctgagctgc     3060

tctgacgcgg ccgtctggtg cgtttcactg atcctggtgc tgcagcttcc ttacacttcc     3120

caagaggaga agcagtttgg aaaaacaaaa tcagaataag ttggtcctga gttct          3175


<210>  63
<211>  500
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct

<400>  63

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Asp 
            20                  25                  30          


Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly Asp 
        35                  40                  45              


Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr Leu 
    50                  55                  60                  


Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile Tyr 
65                  70                  75                  80  


His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 
                85                  90                  95      


Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln Glu 
            100                 105                 110         


Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr Thr 
        115                 120                 125             


Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu Ser 
145                 150                 155                 160 


Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys Thr 
                165                 170                 175     


Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln 
            180                 185                 190         


Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser Glu 
        195                 200                 205             


Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys 
    210                 215                 220                 


Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr 
225                 230                 235                 240 


Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly 
                245                 250                 255     


Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser 
            260                 265                 270         


Ser Pro Gly Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro 
        275                 280                 285             


Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys 
    290                 295                 300                 


Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala 
305                 310                 315                 320 


Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu 
                325                 330                 335     


Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys 
            340                 345                 350         


Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr 
        355                 360                 365             


Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly 
    370                 375                 380                 


Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala 
385                 390                 395                 400 


Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg 
                405                 410                 415     


Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu 
            420                 425                 430         


Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
        435                 440                 445             


Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
    450                 455                 460                 


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
465                 470                 475                 480 


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
                485                 490                 495     


Leu Pro Pro Arg 
            500 


<210>  64
<211>  3199
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR construct

<400>  64
accgtttttc tggacaaccc caaagtaccc cgtctccctg gctttagcca cctctccatc       60

ctcttgcttt ctttgcctgg acaccccgtt ctcctgtgga ttcgggtcac ctctcactcc      120

tttcatttgg gcagctcccc tacccccctt acctctctag tctgtgctag ctcttccagc      180

cccctgtcat ggcatcttcc aggggtccga gagctcagct agtcttcttc ctccaacccg      240

ggcccctatg tccacttcag gacagcatgt ttgctgcctc cagggatcct gtgtccccga      300

gctgggacca ccttatattc ccagggccgg ttaatgtggc tctggttctg ggtactttta      360

tctgtcccct ccaccccaca gtggggccac tagggacagc attgattatt gagtagttat      420

taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca      480

taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca      540

ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg      600

gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg      660

ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc      720

ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg      780

atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca      840

agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt      900

ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg      960

gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg     1020

ccaccatggc tcttcctgtg actgcccttc tgctgcccct ggctctcctg ctccatgctg     1080

cccggccaga gcaaaaactt atctctgaag aggacctcga catccagatg acacagacaa     1140

ccagcagcct cagcgccagc ctgggggaca gagtgaccat tagctgccgg gcctctcagg     1200

acatcagcaa atacctgaac tggtaccagc agaaaccaga tggcactgtc aagctgctga     1260

tttaccacac ctccaggctc cacagcggcg tgcccagtcg cttcagcggc agtgggagcg     1320

ggacagatta ttccctcaca atctccaacc tggagcagga agatattgcc acatacttct     1380

gccagcaagg caacaccctg ccatacacat ttggaggcgg caccaaattg gagatcaccg     1440

gcggtggtgg atctggagga gggggcagcg gaggtggcgg ctctgaggtg aaactgcagg     1500

agagtggccc tggcctggtg gctcccagcc agagcctttc tgtcacctgc accgtgtctg     1560

gggtgtccct gcctgactat ggagtctcct ggatccggca gcctccaaga aaaggactgg     1620

aatggctggg cgtcatctgg ggaagtgaga ccacctacta taattcagcc ctcaagtccc     1680

ggctcaccat cattaaggac aactccaaat cccaggtgtt cctgaagatg aattctctcc     1740

agactgatga cacagccatc tactactgtg ccaagcatta ttattacggc gggtcctatg     1800

ccatggacta ctggggccag gggaccagtg tcactgtttc ttctcccggg aagccgacca     1860

caacaccagc accgcgccca ccaacaccag ctcccacaat agcctcgcag ccgctctcac     1920

tgcgcccgga ggcttgtagg cccgctgccg gtggagcagt tcatacgcga ggacttgatt     1980

tcgcctgtga catatatatt tgggcacctc tcgccggaac ctgtggggtc ttgctgctgt     2040

ctctcgttat aacgctttat tgcaaacggg gcagaaagaa actcctgtat atattcaaac     2100

aaccatttat gagaccagta caaactactc aagaggaaga tggctgtagc tgccgatttc     2160

cagaagaaga agaaggagga tgtgaactga gggtcaaatt ttctagatct gcagatgcgc     2220

cggcctatca acaaggccag aaccagctct ataacgagct caatctagga cgaagagagg     2280

agtacgatgt tttggacaag agacgtggcc gggaccctga gatgggggga aagccgagaa     2340

ggaagaaccc tcaggaaggc ctgtacaatg aactgcagaa agataagatg gcggaggcct     2400

acagtgagat tgggatgaaa ggcgagcgcc ggaggggcaa ggggcacgat ggcctttacc     2460

agggtctcag tacagccacc aaggacacct acgacgccct tcacatgcag gccctgcccc     2520

ctcgctaaag atctcgagtc tagctcgagg gcgcgccccg ctgatcagcc tcgactgtgc     2580

cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag     2640

gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta     2700

ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag     2760

acaatagcag gcatgctggg gatgcggtgg gctctatggg attggtgaca gaaaagcccc     2820

atccttaggc ctcctccttc ctagtctcct gatattgggt ctaaccccca cctcctgtta     2880

ggcagattcc ttatctggtg acacaccccc atttcctgga gccatctctc tccttgccag     2940

aacctctaag gtttgcttac gatggagcca gagaggatcc tgggagggag agcttggcag     3000

ggggtgggag ggaagggggg gatgcgtgac ctgcccggtt ctcagtggcc accctgcgct     3060

accctctccc agaacctgag ctgctctgac gcggccgtct ggtgcgtttc actgatcctg     3120

gtgctgcagc ttccttacac ttcccaagag gagaagcagt ttggaaaaac aaaatcagaa     3180

taagttggtc ctgagttct                                                  3199


<210>  65
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  guide RNA

<400>  65
ggccacggag cgagacaucu                                                   20


