                         SEQUENCE LISTING

<110>  Syngenta Crop Protection AG
 
<120>  PESTICIDAL HEXATOXIN POLYPEPTIDES AND METHODS OF USE THEREOF

<130>  P302654WO

<150>  EP20216063.6
<151>  2020-12-21

<160>  38    

<170>  PatentIn version 3.5

<210>  1
<211>  37
<212>  PRT
<213>  Hadronyche versuta

<400>  1

Ser Pro Thr Cys Ile Pro Ser Gly Gln Pro Cys Pro Tyr Asn Glu Asn 
1               5                   10                  15      


Cys Cys Ser Gln Ser Cys Thr Phe Lys Glu Asn Glu Asn Gly Asn Thr 
            20                  25                  30          


Val Lys Arg Cys Asp 
        35          


<210>  2
<211>  36
<212>  PRT
<213>  Atrax robustus

<400>  2

Gly Ala Cys Thr Pro Thr Gly Gln Pro Cys Pro Tyr Asn Glu Ser Cys 
1               5                   10                  15      


Cys Ser Gly Ser Cys Gln Glu Gln Leu Asn Glu Asn Gly His Thr Val 
            20                  25                  30          


Lys Arg Cys Val 
        35      


<210>  3
<211>  37
<212>  PRT
<213>  Hadronyche modesta

<400>  3

Ala Pro Val Cys Thr Arg Thr Asp Gln Pro Cys Pro Tyr Asn Glu Asp 
1               5                   10                  15      


Cys Cys Ser Gly Ser Cys Thr Leu Lys Lys Asn Glu Asn Gly Asn Leu 
            20                  25                  30          


Val Lys Arg Cys Asp 
        35          


<210>  4
<211>  37
<212>  PRT
<213>  Hadronyche modesta

<400>  4

Ser Pro Val Cys Thr Arg Thr Asp Gln Pro Cys Pro Tyr Asp Gln Asp 
1               5                   10                  15      


Cys Cys Ser Gly Ser Cys Thr Leu Lys Lys Asn Glu Asn Gly Asn Leu 
            20                  25                  30          


Val Lys Arg Cys Asp 
        35          


<210>  5
<211>  37
<212>  PRT
<213>  Hadronyche modesta

<400>  5

Ala Pro Val Cys Thr Arg Thr Asp Gln Pro Cys Pro Tyr Asp Gln Asp 
1               5                   10                  15      


Cys Cys Ser Gly Ser Cys Thr Leu Lys Lys Asn Glu Asn Gly Asn Leu 
            20                  25                  30          


Val Lys Arg Cys Asp 
        35          


<210>  6
<211>  36
<212>  PRT
<213>  Hadronyche infensa

<400>  6

Ser Thr Cys Thr Pro Thr Asp Gln Pro Cys Pro Tyr His Glu Ser Cys 
1               5                   10                  15      


Cys Ser Gly Ser Cys Thr Tyr Lys Ala Asn Glu Asn Gly Asn Gln Val 
            20                  25                  30          


Lys Arg Cys Asp 
        35      


<210>  7
<211>  36
<212>  PRT
<213>  Hadronyche infensa

<400>  7

Ser Thr Cys Thr Pro Thr Asp Gln Pro Cys Pro Tyr Asp Glu Ser Cys 
1               5                   10                  15      


Cys Ser Gly Ser Cys Thr Tyr Lys Ala Asn Glu Asn Gly Asn Gln Val 
            20                  25                  30          


Lys Arg Cys Asp 
        35      


<210>  8
<211>  37
<212>  PRT
<213>  Hadronyche infensa

<400>  8

Ser Ser Thr Cys Ile Arg Thr Asp Gln Pro Cys Pro Tyr Asn Glu Ser 
1               5                   10                  15      


Cys Cys Ser Gly Ser Cys Thr Tyr Lys Ala Asn Glu Asn Gly Asn Gln 
            20                  25                  30          


Val Lys Arg Cys Asp 
        35          


<210>  9
<211>  37
<212>  PRT
<213>  Hadronyche modesta

<400>  9

Ser Thr Val Cys Ile Pro Ser Gly Gln Pro Cys Pro Tyr Ser Lys Tyr 
1               5                   10                  15      


Cys Cys Ser Gly Ser Cys Thr Tyr Lys Glu Asn Glu Asn Gly Asn Thr 
            20                  25                  30          


Val Gln Arg Cys Asp 
        35          


<210>  10
<211>  37
<212>  PRT
<213>  Hadronyche versuta

<400>  10

Ser Ala Val Cys Ile Pro Ser Gly Gln Pro Cys Pro Tyr Ser Lys Tyr 
1               5                   10                  15      


Cys Cys Ser Gly Ser Cys Thr Tyr Lys Thr Asn Glu Asn Gly Asn Ser 
            20                  25                  30          


Val Gln Arg Cys Asp 
        35          


<210>  11
<211>  37
<212>  PRT
<213>  Hadronyche infensa

<400>  11

Ser Pro Thr Cys Ile Pro Thr Gly Gln Pro Cys Pro Tyr Asn Glu Asn 
1               5                   10                  15      


Cys Cys Asn Gln Ser Cys Thr Tyr Lys Ala Asn Glu Asn Gly Asn Gln 
            20                  25                  30          


Val Lys Arg Cys Asp 
        35          


<210>  12
<211>  37
<212>  PRT
<213>  Hadronyche infensa

<400>  12

Ser Pro Thr Cys Ile Pro Thr Gly Gln Pro Cys Pro Tyr Asn Glu Asn 
1               5                   10                  15      


Cys Cys Ser Gln Ser Cys Thr Tyr Lys Ala Asn Glu Asn Gly Asn Gln 
            20                  25                  30          


Val Lys Arg Cys Asp 
        35          


<210>  13
<211>  37
<212>  PRT
<213>  Hadronyche infensa

<400>  13

Ser Pro Thr Cys Ile Pro Thr Gly Gln Pro Cys Pro Tyr Asn Glu Asn 
1               5                   10                  15      


Cys Cys Ser Gln Ser Cys Thr Tyr Lys Thr Asn Glu Asn Gly Asn Gln 
            20                  25                  30          


Val Lys Gly Cys Asp 
        35          


<210>  14
<211>  37
<212>  PRT
<213>  Hadronyche infensa

<400>  14

Ser Pro Thr Cys Ile Pro Thr Gly Gln Pro Cys Pro Tyr Asn Glu Asn 
1               5                   10                  15      


Cys Cys Ser Gln Ser Cys Thr Tyr Lys Ala Asn Glu Asn Gly Asn Gln 
            20                  25                  30          


Val Lys Gly Cys Asp 
        35          


<210>  15
<211>  37
<212>  PRT
<213>  Hadronyche modesta

<400>  15

Ser Pro Val Cys Ile Pro Ser Gly Gln Pro Cys Pro Tyr Asn Glu His 
1               5                   10                  15      


Cys Cys Ser Gly Ser Cys Thr Tyr Lys Glu Asn Glu Asn Gly Asn Thr 
            20                  25                  30          


Val Gln Arg Cys Asp 
        35          


<210>  16
<211>  37
<212>  PRT
<213>  Atrax robustus

<400>  16

Ser Ser Val Cys Ile Pro Ser Gly Gln Pro Cys Pro Tyr Asn Glu His 
1               5                   10                  15      


Cys Cys Ser Gly Ser Cys Thr Tyr Lys Glu Asn Glu Asn Gly Asn Thr 
            20                  25                  30          


Val Gln Arg Cys Asp 
        35          


<210>  17
<211>  37
<212>  PRT
<213>  Atrax robustus

<400>  17

Ser Pro Thr Cys Ile Pro Ser Gly Gln Pro Cys Pro Tyr Asn Glu Asn 
1               5                   10                  15      


Cys Cys Ser Lys Ser Cys Thr Tyr Lys Glu Asn Glu Asn Gly Asn Thr 
            20                  25                  30          


Val Gln Arg Cys Asp 
        35          


<210>  18
<211>  37
<212>  PRT
<213>  Hadronyche venenata

<400>  18

Ser Pro Thr Cys Ile Pro Ser Gly Gln Pro Cys Pro Tyr Asn Glu Asn 
1               5                   10                  15      


Cys Cys Ser Lys Ser Cys Thr Tyr Lys Glu Asn Glu Asn Gly Asn Thr 
            20                  25                  30          


Val Gln Arg Cys Asp 
        35          


<210>  19
<211>  37
<212>  PRT
<213>  Hadronyche versuta

<400>  19

Ser Pro Thr Cys Ile Pro Ser Gly Gln Pro Cys Pro Tyr Asn Glu Asn 
1               5                   10                  15      


Cys Cys Ser Lys Ser Cys Thr Tyr Lys Glu Asn Glu Asn Gly Asn Thr 
            20                  25                  30          


Val Gln Arg Cys Asp 
        35          


<210>  20
<211>  37
<212>  PRT
<213>  Hadronyche formidabilis

<400>  20

Ser Pro Thr Cys Ile Arg Ser Gly Gln Pro Cys Pro Tyr Asn Glu Asn 
1               5                   10                  15      


Cys Cys Ser Gln Ser Cys Thr Phe Lys Thr Asn Glu Asn Gly Asn Thr 
            20                  25                  30          


Val Lys Arg Cys Asp 
        35          


<210>  21
<211>  37
<212>  PRT
<213>  Hadronyche versuta

<400>  21

Ser Ser Thr Cys Ile Pro Ser Gly Gln Pro Cys Pro Tyr Asn Glu Asn 
1               5                   10                  15      


Cys Cys Ser Gln Ser Cys Thr Tyr Lys Glu Asn Glu Asn Gly Asn Thr 
            20                  25                  30          


Val Lys Arg Cys Asp 
        35          


<210>  22
<211>  37
<212>  PRT
<213>  Hadronyche versuta

<400>  22

Ser Ser Thr Cys Ile Pro Ser Gly Gln Pro Cys Pro Tyr Asn Glu Asn 
1               5                   10                  15      


Cys Cys Ser Gln Ser Cys Thr Phe Lys Glu Asn Glu Asn Gly Asn Thr 
            20                  25                  30          


Val Lys Arg Cys Asp 
        35          


<210>  23
<211>  37
<212>  PRT
<213>  Atrax robustus

<400>  23

Ser Pro Thr Cys Ile Pro Ser Gly Gln Pro Cys Pro Tyr Asn Glu Asn 
1               5                   10                  15      


Cys Cys Ser Gln Ser Cys Thr Phe Lys Glu Asn Glu Asn Gly Asn Thr 
            20                  25                  30          


Val Lys Arg Cys Asp 
        35          


<210>  24
<211>  37
<212>  PRT
<213>  Hadronyche versuta

<400>  24

Ser Pro Thr Cys Ile Pro Ser Gly Gln Pro Cys Pro Tyr Asn Glu Asn 
1               5                   10                  15      


Cys Cys Ser Gln Ser Cys Thr Tyr Lys Glu Asn Glu Asn Gly Asn Thr 
            20                  25                  30          


Val Lys Arg Cys Asp 
        35          


<210>  25
<211>  39
<212>  PRT
<213>  Hadronyche versuta

<400>  25

Gln Tyr Cys Val Pro Val Asp Gln Pro Cys Ser Leu Asn Thr Gln Pro 
1               5                   10                  15      


Cys Cys Asp Asp Ala Thr Cys Thr Gln Glu Arg Asn Glu Asn Gly His 
            20                  25                  30          


Thr Val Tyr Tyr Cys Arg Ala 
        35                  


<210>  26
<211>  39
<212>  PRT
<213>  Missulena bradleyi

<400>  26

Ser Pro Val Cys Thr Pro Ser Gly Gln Pro Cys Gln Pro Asn Thr Gln 
1               5                   10                  15      


Pro Cys Cys Asn Asn Ala Glu Glu Glu Gln Thr Ile Asn Cys Asn Gly 
            20                  25                  30          


Asn Thr Val Tyr Arg Cys Ala 
        35                  


<210>  27
<211>  37
<212>  PRT
<213>  Hadronyche versuta

<400>  27

Ala Ile Cys Pro Gly Ala Asp Arg Pro Cys Ala Ala Cys Cys Pro Cys 
1               5                   10                  15      


Cys Pro Gly Thr Ser Cys Lys Ala Glu Ser Asn Gly Val Phe Tyr Cys 
            20                  25                  30          


Arg Lys Asp Glu Pro 
        35          


<210>  28
<211>  37
<212>  PRT
<213>  Hadronyche versuta

<400>  28

Val Ile Cys Thr Gly Ala Asp Ser Pro Cys Ala Ala Cys Cys Pro Cys 
1               5                   10                  15      


Cys Pro Gly Thr Ser Cys Lys Ala Glu Ser Asn Gly Val Ser Tyr Cys 
            20                  25                  30          


Arg Lys Asp Glu Pro 
        35          


<210>  29
<211>  37
<212>  PRT
<213>  Hadronyche versuta

<400>  29

Ala Ile Cys Thr Gly Ala Asp Arg Pro Cys Ala Ala Cys Cys Pro Cys 
1               5                   10                  15      


Cys Pro Gly Thr Ser Cys Lys Ala Glu Ser Asn Gly Val Ser Tyr Cys 
            20                  25                  30          


Arg Lys Asp Glu Pro 
        35          


<210>  30
<211>  36
<212>  PRT
<213>  Hadronyche versuta

<400>  30

Thr Ile Cys Thr Gly Ala Asp Arg Pro Cys Ala Ala Cys Cys Pro Cys 
1               5                   10                  15      


Cys Pro Gly Thr Ser Cys Gln Gly Pro Glu Ser Asn Gly Val Val Tyr 
            20                  25                  30          


Cys Arg Asn Phe 
        35      


<210>  31
<211>  37
<212>  PRT
<213>  Hadronyche formidabilis

<400>  31

Ser Pro Thr Cys Thr Gly Ala Asp Arg Pro Cys Ala Ala Cys Cys Pro 
1               5                   10                  15      


Cys Cys Pro Gly Thr Ser Cys Lys Gly Pro Glu Pro Asn Gly Val Ser 
            20                  25                  30          


Tyr Cys Arg Asn Asp 
        35          


<210>  32
<211>  36
<212>  PRT
<213>  Hadronyche versuta

<400>  32

Thr Ile Cys Thr Gly Ala Asp Arg Pro Cys Ala Ala Cys Cys Pro Cys 
1               5                   10                  15      


Cys Pro Gly Thr Ser Cys Gln Gly Pro Glu Pro Asn Gly Val Ser Tyr 
            20                  25                  30          


Cys Arg Asn Asp 
        35      


<210>  33
<211>  37
<212>  PRT
<213>  Atrax robustus

<400>  33

Ser Pro Thr Cys Ile Pro Ser Gly Gln Pro Cys Pro Tyr Asn Glu Asn 
1               5                   10                  15      


Cys Cys Ser Gln Ser Cys Thr Phe Lys Glu Asn Glu Thr Gly Asn Thr 
            20                  25                  30          


Val Lys Arg Cys Asp 
        35          


<210>  34
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  derived from Hadronyche versuta to which amino acids GS are added

<400>  34

Gly Ser Gln Tyr Cys Val Pro Val Asp Gln Pro Cys Ser Leu Asn Thr 
1               5                   10                  15      


Gln Pro Cys Cys Asp Asp Ala Thr Cys Thr Gln Glu Arg Asn Glu Asn 
            20                  25                  30          


Gly His Thr Val Tyr Tyr Cys Arg Ala 
        35                  40      


<210>  35
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant 1 derived from w-hexatoxin-Hv1a

<400>  35

Ser Pro Thr Cys Ile Pro Ser Gly Gln Pro Cys Pro Tyr Asn Glu Asn 
1               5                   10                  15      


Cys Cys Ser Gln Ser Cys Thr Phe Lys Glu Asn Glu Ala Gly Asn Thr 
            20                  25                  30          


Val Lys Arg Cys Asp 
        35          


<210>  36
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant 2 derived from w-hexatoxin-Hv1a

<400>  36

Ser Pro Thr Cys Ile Pro Ser Gly Gln Pro Cys Pro Tyr Asn Glu Asn 
1               5                   10                  15      


Cys Cys Ser Gln Ser Cys Thr Phe Lys Glu Asn Glu Leu Gly Asn Thr 
            20                  25                  30          


Val Lys Arg Cys Asp 
        35          


<210>  37
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant 3 derived from k-hexatoxin-Hv1c

<400>  37

Ala Ile Cys Thr Gly Ala Asp Arg Pro Cys Ala Ala Cys Cys Pro Cys 
1               5                   10                  15      


Cys Pro Gly Thr Ser Cys Lys Ala Glu Ser Leu Gly Val Ser Tyr Cys 
            20                  25                  30          


Arg Lys Asp Glu Pro 
        35          


<210>  38
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant 4 derived from GS-w/k-hexatoxin-Hv1h

<400>  38

Gly Ser Gln Tyr Cys Val Pro Val Asp Gln Pro Cys Ser Leu Asn Thr 
1               5                   10                  15      


Gln Pro Cys Cys Asp Asp Ala Thr Cys Thr Gln Glu Arg Asn Glu Leu 
            20                  25                  30          


Gly His Thr Val Tyr Tyr Cys Arg Ala 
        35                  40      


