               SEQUENCE LISTING

<110> BASF SE

<120> Means and methods for manufacturing racemic alanine for the MGDA synthesis

<130> B202123PC

<160> 4

<170> BiSSAP 1.3.6

<210> 1
<211> 388
<212> PRT
<213> Caldanaerobacter subterraneus subsp. tengcongensis


<400> 1
Met Phe Asp Glu Ile Arg Pro Thr Arg Val Glu Val Asn Leu Asp Ser 
1               5                   10                  15      
Ile Val His Asn Phe Arg Glu Ile Lys Arg Val Val Gly Asp Arg Val 
            20                  25                  30          
Lys Val Met Gly Val Val Lys Ala Asn Ala Tyr Gly His Gly Ala Tyr 
        35                  40                  45              
His Val Ala Lys Ala Leu Val Glu Asn Gly Val Asp Tyr Leu Ala Val 
    50                  55                  60                  
Ala Thr Val Glu Glu Ala Leu Glu Leu Arg Ser Tyr Gly Ile Thr Ala 
65                  70                  75                  80  
Pro Val Leu Ile Leu Gly Tyr Thr Pro Leu Ser Gln Ala Gly Glu Ala 
                85                  90                  95      
Val Glu Lys Asp Val Thr Phe Thr Ala Phe Asp Leu Lys Tyr Val Lys 
            100                 105                 110         
Glu Leu Gly Glu Ile Ala Ser Arg Lys Gly Lys Lys Ala Lys Ile His 
        115                 120                 125             
Val Lys Ile Asp Thr Gly Met Gly Arg Ile Gly Tyr Thr Asp Phe Asp 
    130                 135                 140                 
Leu Ala Glu Arg Glu Ile Glu Glu Met Ser Lys Leu Glu Gly Ile Glu 
145                 150                 155                 160 
Leu Glu Gly Ile Phe Ser His Phe Ala Thr Ser Asp Glu Lys Asp Lys 
                165                 170                 175     
Asp Tyr Ala Arg Glu Gln Phe Glu Arg Phe Ala Asp Met Leu Lys Arg 
            180                 185                 190         
Leu Glu Lys Arg Gly Val Asn Ile Thr Leu Lys His Ile Ala Asn Ser 
        195                 200                 205             
Gly Ala Ile Thr Asp Leu Asn Tyr Ala Tyr Leu Asp Met Val Arg Pro 
    210                 215                 220                 
Gly Ile Thr Leu Tyr Gly Ser Tyr Pro Ser Asn Asp Val Asn Lys Ile 
225                 230                 235                 240 
Leu Asp Leu Arg Pro Ala Met Asn Phe Lys Thr Lys Ile Val Tyr Ile 
                245                 250                 255     
Lys Glu Val Pro Glu Asn Thr Ser Ile Ser Tyr Gly Arg Thr Phe Ile 
            260                 265                 270         
Thr Lys Arg Pro Ser Lys Ile Ala Thr Leu Pro Ile Gly Tyr Ala Asp 
        275                 280                 285             
Gly Leu Asn Arg Leu Leu Ser Asn Asn His Glu Val Leu Val Arg Gly 
    290                 295                 300                 
Lys Tyr Val Pro Ile Val Gly Arg Val Cys Met Asp Gln Thr Met Ile 
305                 310                 315                 320 
Asp Val Thr Glu Val Glu Gly Val Glu Val Gly Asp Glu Val Val Ile 
                325                 330                 335     
Phe Gly Glu Gln Glu Gly Lys Arg Ile Thr Ala Asp Asp Ile Ala Lys 
            340                 345                 350         
Lys Leu Arg Thr Ile Pro His Glu Val Tyr Cys Gly Ile Ser Arg Arg 
        355                 360                 365             
Val Pro Arg Ile Tyr Ile Tyr Arg Gly Glu Val Phe Asp Val Lys Asn 
    370                 375                 380                 
Tyr Leu Lys Ile 
385             

<210> 2
<211> 388
<212> PRT
<213> Geobacillus stearothermophilus


<400> 2
Met Asn Asp Phe His Arg Asp Thr Trp Ala Glu Val Asp Leu Asp Ala 
1               5                   10                  15      
Ile Tyr Asp Asn Val Glu Asn Leu Arg Arg Leu Leu Pro Asp Asp Thr 
            20                  25                  30          
His Ile Met Ala Val Val Lys Ala Asn Ala Tyr Gly His Gly Asp Val 
        35                  40                  45              
Gln Val Ala Arg Thr Ala Leu Glu Ala Gly Ala Ser Arg Leu Ala Val 
    50                  55                  60                  
Ala Phe Leu Asp Glu Ala Leu Ala Leu Arg Glu Lys Gly Ile Glu Ala 
65                  70                  75                  80  
Pro Ile Leu Val Leu Gly Ala Ser Arg Pro Ala Asp Ala Ala Leu Ala 
                85                  90                  95      
Ala Gln Gln Arg Ile Ala Leu Thr Val Phe Arg Ser Asp Trp Leu Glu 
            100                 105                 110         
Glu Ala Ser Ala Leu Tyr Ser Gly Pro Phe Pro Ile His Phe His Leu 
        115                 120                 125             
Lys Met Asp Thr Gly Met Gly Arg Leu Gly Val Lys Asp Glu Glu Glu 
    130                 135                 140                 
Thr Lys Arg Ile Val Ala Leu Ile Glu Arg His Pro His Phe Val Leu 
145                 150                 155                 160 
Glu Gly Leu Tyr Thr His Phe Ala Thr Ala Asp Glu Val Asn Thr Asp 
                165                 170                 175     
Tyr Phe Ser Tyr Gln Tyr Thr Arg Phe Leu His Met Leu Glu Trp Leu 
            180                 185                 190         
Pro Ser Arg Pro Pro Leu Val His Cys Ala Asn Ser Ala Ala Ser Leu 
        195                 200                 205             
Arg Phe Pro Asp Arg Thr Phe Asn Met Val Arg Phe Gly Ile Ala Met 
    210                 215                 220                 
Tyr Gly Leu Ala Pro Ser Pro Gly Ile Lys Pro Leu Leu Pro Tyr Pro 
225                 230                 235                 240 
Leu Lys Glu Ala Phe Ser Leu His Ser Arg Leu Val His Val Lys Lys 
                245                 250                 255     
Leu Gln Pro Gly Glu Lys Val Ser Tyr Gly Ala Thr Tyr Thr Ala Gln 
            260                 265                 270         
Thr Glu Glu Trp Ile Gly Thr Ile Pro Ile Gly Tyr Ala Asp Gly Trp 
        275                 280                 285             
Leu Arg Arg Leu Gln His Phe His Val Leu Val Asp Gly Gln Lys Ala 
    290                 295                 300                 
Pro Ile Val Gly Arg Ile Cys Met Asp Gln Cys Met Ile Arg Leu Pro 
305                 310                 315                 320 
Gly Pro Leu Pro Val Gly Thr Lys Val Thr Leu Ile Gly Arg Gln Gly 
                325                 330                 335     
Asp Glu Val Ile Ser Ile Asp Asp Val Ala Arg His Leu Glu Thr Ile 
            340                 345                 350         
Asn Tyr Glu Val Pro Cys Thr Ile Ser Tyr Arg Val Pro Arg Ile Phe 
        355                 360                 365             
Phe Arg His Lys Arg Ile Met Glu Val Arg Asn Ala Ile Gly Arg Gly 
    370                 375                 380                 
Glu Ser Ser Ala 
385             

<210> 3
<211> 341
<212> PRT
<213> Aquifex pyrophilus


<400> 3
Met Arg Arg Ala Val Leu Glu Ile Leu Glu Glu Arg Ile Ile His Asn 
1               5                   10                  15      
Val Lys Glu Ile His Arg Phe Ser Gly Lys Arg Ile Ile Ala Val Val 
            20                  25                  30          
Lys Ala Asn Ala Tyr Gly Ile Gly Val Arg Glu Val Ser Arg Ile Leu 
        35                  40                  45              
Glu Gly Leu Glu Glu Val Asp Ala Phe Ala Val Ala Cys Thr Gln Glu 
    50                  55                  60                  
Gly Val Glu Leu Arg Glu Cys Gly Ile Lys Lys Lys Ile Leu Ile Leu 
65                  70                  75                  80  
Gly Gly Ile Leu Glu Glu Asp Val Lys Leu Leu Glu Glu Tyr Asp Leu 
                85                  90                  95      
Thr Pro Val Ile Ser Asp Pro Glu His Leu Lys Val Leu Lys Asp Arg 
            100                 105                 110         
Asn Ile Lys Phe His Val Lys Tyr Asp Thr Gly Met Gly Arg Leu Gly 
        115                 120                 125             
Phe Thr Asn Glu Ile Ile Lys Asp Pro Arg Val Glu Gly Val Met Ser 
    130                 135                 140                 
His Phe Ser Ser Pro Ala Asp Arg Asn Phe Ser Lys Leu Gln Ile Lys 
145                 150                 155                 160 
Arg Phe Glu Glu Ile Leu Lys Asn Tyr Glu Lys Val Lys Tyr Ile His 
                165                 170                 175     
Leu Glu Ser Ser Ala Gly Leu Ile Tyr Arg Val Pro Phe Thr Thr His 
            180                 185                 190         
Val Arg Val Gly Leu Ala Ile Tyr Gly Glu Lys Pro Leu Lys Asp Tyr 
        195                 200                 205             
Pro Leu Glu Val Lys Pro Ala Leu Arg Leu Arg Ala Arg Leu Ile Ser 
    210                 215                 220                 
Val Lys Glu Leu Pro Glu Asn Tyr Pro Val Ser Tyr Gly Arg Thr Tyr 
225                 230                 235                 240 
Ile Thr Lys Arg Lys Thr Lys Leu Gly Val Val Ala Phe Gly Tyr Ala 
                245                 250                 255     
Asp Gly Leu Met Lys Thr Leu Ser Asn Arg Ser Phe Leu Ile Phe Glu 
            260                 265                 270         
Gly Arg Lys Val Pro Ile Ile Gly Asn Ile Thr Met Asp Met Thr Met 
        275                 280                 285             
Val Asp Leu Ser Gly Thr Glu Ala Arg Thr Gly Asp Trp Val Tyr Ile 
    290                 295                 300                 
Val Asn Glu Glu Arg Ser Phe Thr Pro Leu Ala Arg Asp Ala Gly Thr 
305                 310                 315                 320 
Ile Pro Tyr Glu Ile Met Cys Asn Leu Ser Arg Arg Val Glu Arg Leu 
                325                 330                 335     
Val Ile Lys Lys Arg 
            340     

<210> 4
<211> 369
<212> PRT
<213> Bacillus pseudofirmus


<400> 4
Met Lys Thr Ser Ser Phe Arg Asn Thr Tyr Ala Gln Ile Ser Leu Gln 
1               5                   10                  15      
Ala Leu Lys Glu Asn Ala Ala Ser Phe Lys Ala Ser Leu Gln Ser Pro 
            20                  25                  30          
Ala Cys Arg Leu Met Ala Val Val Lys Gly Asp Gly Tyr Gly His Gly 
        35                  40                  45              
Ala Val Ala Ala Ala Ser Ser Ala Leu Asn Gly Gly Ala Asp Tyr Leu 
    50                  55                  60                  
Gly Val Ala Ile Leu Asp Glu Ala Ile Glu Leu Arg Asp Ala Gly Val 
65                  70                  75                  80  
Glu Ala Pro Ile Leu Val Leu Gly Tyr Thr Ser Pro His Ala Leu Arg 
                85                  90                  95      
Glu Ala Ile Ser Arg Asn Ile Thr Leu Thr Val Phe Ser Thr Asp Val 
            100                 105                 110         
Arg Asp Ala Leu Leu Glu Val Ala Ser Glu Ala Glu Ser Pro Ile Lys 
        115                 120                 125             
Val His Ile Lys Thr Glu Thr Gly Met Gly Arg Val Gly Val Gln Thr 
    130                 135                 140                 
Lys Glu Glu Leu Leu Asp Val Met Thr Pro Leu Tyr His His Asn Asn 
145                 150                 155                 160 
Ile Glu Val Glu Gly Ile Phe Thr His Phe Ala Glu Ala Asp Asn Leu 
                165                 170                 175     
Gln Ser Thr Tyr Thr Asp Glu Gln Phe Ala Arg Phe Leu Ser Phe Ile 
            180                 185                 190         
Glu Ala Ile Glu Lys Asp Asp Met His Val Pro Ile Lys His Cys Cys 
        195                 200                 205             
Asn Ser Ala Gly Thr Leu Phe His Lys Asp Lys His Leu Asp Met Val 
    210                 215                 220                 
Arg Val Gly Ile Ser Leu Tyr Gly Leu Arg Pro Asp Val Ser Leu Glu 
225                 230                 235                 240 
Phe Pro Ile Glu Leu Thr Gln Ala Met Arg Leu Phe Ser Ser Ile Val 
                245                 250                 255     
Ser Leu Arg Lys Leu Pro Glu Gly Ser Ser Ile Ser Tyr Gly Arg Thr 
            260                 265                 270         
His Lys Leu Ser Ser Glu Lys Val Val Ala Thr Met Pro Ile Gly Tyr 
        275                 280                 285             
Ala Asp Gly Leu Ser Arg Ala Leu Ser Asn Lys Gly Phe Val Thr Leu 
    290                 295                 300                 
His Gly Gln Lys Ala Pro Ile Leu Gly Arg Val Cys Met Asp Gln Thr 
305                 310                 315                 320 
Met Ile Asp Val Thr Asp Ile Pro Asp Ala Ala Leu Gly Asp His Val 
                325                 330                 335     
Glu Phe Pro Ile Asp Glu Met Ala Glu Leu Thr Gly Thr Ile Asn Tyr 
            340                 345                 350         
Glu Ile Val Cys Ala Val Ser Lys Arg Val Pro Arg Tyr Tyr Glu Glu 
        355                 360                 365             
Asn 
    

