﻿               SEQUENCE LISTING

<110> Consejo Superior de Investigaciones Científicas(CSIC)

<120> Recombinant bacterium and uses thereof

<130> PCT1641.1637

<150> EP20383142
<151> 2020-12-22

<160> 71

<170> BiSSAP 1.3.6

<210> 1
<211> 5472
<212> DNA
<213> Artificial Sequence


<220> 
<223> Sequence of eLEE operon 1

<400> 1
cctgtagtac gggaagctgc ttagaacact atatataggg ggtatgtttg ggattcactg       60

caagatagtg tgaaaatgac cctcttgcaa gtgcataact ttgtggataa ctcaggaagg      120

aaaaagtggc tttcgcgcac cttaggtcag acaaggtgtc cgggaaagtc aatgggaaga      180

gaaaaatttg ttaaaaataa ctgttcggcg atttcttgaa cggcataata ggttagataa      240

attgatatag atggcttatt aatctttttg ctaatgattg caaaaattga tctaagcacg      300

agtcatcaca taatctgatg tatcagttgt ggtcaatata taagcgaatg taagagattg      360

ttgatggcga aatggttgtt ttctattcgg ttattgttaa atgctaagcc attttgttaa      420

tttaccttat attataagaa aattattcat gactttaatt tagtttaaat ataactgata      480

tccctgaagg ttatgagctc cagggctaca ctagtcgttg aggttctaga gtcgacaaga      540

aggagatata catatgcgga gattatttat tatgaatatg gaaactaatt cacatacaac      600

aagtccatac attcagctta tagagcaaat tgcagttcta cagcaggaag caaagcgact      660

gcgagagcag gaagttcaaa gtgtaattga gtcgattcag aagcagatta cttattacaa      720

tataacctta caagagctgg gatatactaa tgtgcctgat gatggactcg ctcgccggaa      780

ctcatcgaaa ggtgtttact accgcaatga agaagggcag acctggtcgg gcgtaggccg      840

acagccacgc tggcttaaag aagcactgtt gaatggaatg aagaaagaag attttcttgt      900

gaaggacact gaagaagaaa taataccgct gaaaaatatt taacataaaa taattaaatg      960

ataacgataa ctgagctgga agacgaaata ataaaaaata aagaagccgc aaatgttttt     1020

attgaaaaaa taaacgacaa aaagaacgaa atccatgaaa aaatgaaaca ccctttggat     1080

aaagttacct acaatgaagc aaaggaactg ctcattgcgt gtgatgcggc aattaggact     1140

atagagataa tgcgaatcag gattaataat aaatagagga tgagtattgt gagccaaaca     1200

agaaataaag aactattaga taaaaaaata agatcagaaa ttgaggcgat aaaaaaaata     1260

atcgccgaat ttgatgtcgt aaaagaaagc gttaatgagt taagtgaaaa agcaaaaaca     1320

gatcctcagg cggcagaaaa attaaacaaa ttaattgagg ggtacacata tggtgaagag     1380

cgaaaattat atgattctgc cctaagtaag attgaaaaac taatagagac actgagtcca     1440

gcaagatcta aaagccaatc aacaatgaat caacggaata gaaataatag aaaaatagta     1500

tgacaatttt taataaaata gactataact actataaatt aattaattat ccaatcatga     1560

tggttcatga tgaatggcta ggtgatttaa ccggtgtcaa tcaggtcagt tttcggcgtt     1620

taagagaaac tagttctact cgtaatcaac tcaataagat attgcgtcag gagatacaag     1680

ataaaatttc tggtgtggaa ttatctgaca taaataagga aggtttttta tatcagtcaa     1740

ttgggaaaat aagattgctt gctctaagta gtgcattatt tgacattcaa tgccctgatt     1800

atattttctc acgattatat cgagaaactc tgattagaga gataggatat caaaatgtga     1860

agcagttatc attctattgg cagggagggc aatgtaaacc agaatatggt gaagaacggt     1920

tttgtgctga gttaataaaa tacggtgcag gaaatttgga gtggctattt gctgataatc     1980

ctttatggac catagtcaaa tatcttttac cgaaaagtgg agaaataaaa ccaactcata     2040

ttaatgattt atttcttaac agacttaata aaattttatt gccttatgaa actttatgaa     2100

tttaaaaata tagaaattga tttagtgctt acagaagata tcataccaga ggataagtta     2160

caggaaatta ttcaatctga tgatattatc aaactggctc gaaaaaaaac atatgaacat     2220

ttactgaggg cgaggaggaa atcgaaagaa ttaaagatag aatccagaaa aaaaattgcc     2280

agaaaaatga ttgcaatgag agaaaggatt cgaaaaaata ataaaataaa attagataaa     2340

gaggttaacc aatcgataaa atgggttaaa gatatccagg ctatcgagtt ggtcttaatg     2400

caggacataa tgaataaggt tcatttgtca ctgactaatg cattacattc tttagataca     2460

tcatcaagaa ttaattggga tgatttactt aatgaagttg taagggaaac tctatctaat     2520

aacaatattg tcggagctat taagatcact aaaaatccag acattaaact tgatcctggc     2580

gaggccaaca atatacagct tattaatgat gcaaacacac cgcataataa aattataata     2640

gaaaatgaat atattagaat aactttagac ccattagagc aaataagtat tttattgaat     2700

tcatttaaag ataattatct tagcattatt caggaatgat gatatgtctc aattaatgac     2760

cattggctca cagccaatat ttttaattat tgtatttttt ctgttatcat tactgccaat     2820

atttgttgtt attggtactt cattcctgaa aatttctatt gtactgggga tattgaagaa     2880

cgcattaggc attcaacagg taccaccaaa catggcgcta acatcagtgt ctttgatact     2940

gacaatgttt attatgtctc cgataatatt acagataaat gataatattt ctcaggaacc     3000

aatcaattat accgactctg atttttttca aaaagttgat gagaaaatat tatcaccata     3060

tcgcggtttt ttagaaaaaa atacagagaa agacaatgta gagttttttg aacgtgcagc     3120

tcaaaaaaaa ttgggtaatg aaactatctt aaaaaaagac tctctattta tactgttacc     3180

ggctttcacg atggggcagc ttgaagctgc attcaagata ggttttttgc tctatttacc     3240

ctttattgcg atagatttga tcatttccaa tatcttattg gccttgggta tgatgatggt     3300

atcgccagta acaatttcga ttccttttaa gatcttgtta ttcattcttg ttggaggctg     3360

gcaaaaatta ttcgaatttc tgttggtggt gaattgatgg atactggata ttttgttcaa     3420

ttatgtgtgc aaacgttctg gataatattt atcctctcat tgcctacagt catagcggcc     3480

tctgttatcg gtattattat tagtctggtc caggctataa cgcagttaca ggatcaaaca     3540

ttgccttttt tgctaaaaat aatagcagtg tttgctacgc ttgccctgac ttatcactgg     3600

atgggaacaa caatcatcaa cttctcgtcg ataatatttg aaatgattcc gaaggtgaac     3660

ggctaatgaa tgagataatg acggtcatag tatcatcatt ttattgtata ctcaggccgc     3720

tggggatgtt tattatccta ccaatatttt caacaggtgt tttgctcagt aactttattc     3780

gcaactcaat tatgattgcg tttacgcttc cgatcatagt agagaactat acattttcgg     3840

aaaaattgcc atctggaatc tttcagttga caggaatagt gctaaaagaa ataagtattg     3900

gttttttcat tgggttatca tttactattc ttttttgggc aatagatgcg gctgggcaga     3960

ttattgatac tctaagaggt tcaacaatat cttcaatttt taacccgtcc ataagtgatt     4020

catcttctat cactggcgtt attttgtacc aatttatctc tgtgatcttt gttattcatg     4080

gtgggataca aagcattctg gataagctat atttatccta cgagatatta ccattacaag     4140

ccgatattgc attcaatcgt gctttaatag attttttgtt ttctctatgg gattcattta     4200

ttaaactgat gttatcattt tcagttccca tgattatcgg tatattctta tgtgatatgg     4260

ggtttgggtt tcttaacaaa acagcgcctc aactaaacgt attcacatta tcactgccag     4320

taaaaagttt gatagcaatc tttatattgc tattagttat tcatgttttt cctgatttta     4380

ttacggcaaa tattcattct gacataattg atagatcgtt accgagcatg attaatgagt     4440

gaaaaaacag aaaagcccac acccaaaaaa ctgagggatc taaaaaagaa gggcgatgta     4500

acaaaaagtg aagaggtaat ggctgcagtg cagtcattaa tcttattttc atttttttct     4560

ttatatggca tgagtttttt tgttgatata gttgggttag ttaatacgac aatagactcg     4620

ctaaatagac cgtttttgta tgccattcga gaaatattag gtgcggtgtt aaatatattt     4680

ttattatata ttttgccaat ttctttgatt gtctttgttg gaactgttac gactggtgta     4740

tcacaaatag gattcatctt tgcggttgaa aaaataaaac catcggctca gaagattagt     4800

gtaaaaaata acctgaaaaa tattttttct gtaaagagca tttttgagct acttaaatca     4860

gtatttaagt tagtgataat tgttctcatt ttttatttta tggggcattc atatgcaaat     4920

gagtttgcta atttcacagg actgaacgca tatcaagctc ttgtcgttgt tgcctttttt     4980

gtttttcttt tatggaaagg cgtgctattc ggatatctac tcttttcagt atttgatttc     5040

tggttccaga agcatgaggg actgaagaaa atgaaaatga gtaaagatga ggtgaaacga     5100

gaagccaagg atactgatgg taaccctgaa attaaagggg agcgccgtcg ccttcattcc     5160

gagatacaaa gtggaagttt ggcgaataac atcaaaaaat caaccgttat tgttaaaaac     5220

ccgactcaca ttgcgatttg cctatactat aaacttgggg agactccatt acctttagtt     5280

attgaaacag gaaaagatgc caaagctcta cagatcatta aactggctga actctatgat     5340

attccagtga ttgaagatat tcctttagca agaagtctct ataagaatat acataaagga     5400

caatatataa cagaagactt ttttgaacct gtggcacaat tgattcgtat tgcgatagac     5460

cttgattatt aa                                                         5472


<210> 2
<211> 4623
<212> DNA
<213> Artificial Sequence


<220> 
<223> Sequence of eLEE operon 2

<400> 2
ctcgagggcg atcgcgccag cggagttccc tttattccgt tgcgtgaggt tgctggctgg       60

gaacacgatc ttcatgctgc aatgaacaac attcaggaag aaatcgaatt ggttggcgag      120

aatgcggcga gcattgatgc ttatgcagcc agcgatcctg ctgaatgttt tgccgtactt      180

tctgaatatt tctttagcgc cccagaactt tttgctcctc gtttcccttc attgtggcaa      240

cgtttctgcc aattttatca acaagatcct ttgcagagac tgcatcacgc taatgataca      300

gactcgtttt cggcgacgaa tgttcattaa ttaacaactt tgcagattaa ttaaccaatt      360

gaaatgactt atgaaattta gtgttgacag acaaggtacc gctaagtaat attcgccccg      420

ttcacacgat tcctctgtag ttcagtcggt agaacggcgg actgttaatc cgtatgtcac      480

tggttcgagt ccagtcagag gagccaaatt ctaaaaattc gctgagctct tctgaaatga      540

gctgttgaca attaatcatg ggctcgtata atgtgtggaa ttgtgagcgg ataacaattc      600

tagagtcgac ctgcaggttt gcaagcttaa gaaggagata tacatatgga tgcattatgc      660

tattgcctct ctcatgaaaa gagactaaca gtaaatatga ataatattaa tcaatcggaa      720

aacattaata ttcagttaaa taaagcatcc caaacaaatg tcgttgatga gcatatcccg      780

ttagcatcaa ccccctctgc agccggagcg gctcagtttc ttgatcaatt actccctaaa      840

acggcgggag tgtcttcccc agaacaagtg ttgattgagg aaattaaaaa gcgacatctt      900

gcaacaatga acaccgatct cagtttcgat gcactatctg caggtgggct ctcgcccgaa      960

gatgtactca ctttacaaaa gaatgtgctc aacgcaaacg tcaatgttga tgtcgtatct     1020

aagttagcaa gcctcctttc aacatcggtt acaaaattag tttcgatgca ataaaaatga     1080

aaaaacacat taaaaacctt tttttattgg ctgctatttg tttgacagtg gcatgtaaag     1140

agcaattgta tacaggatta acagaaaaag aagctaatca gatgcaagca ctgttgctat     1200

ccaatgatgt caatgtttcc aaagaaatgg acaaaagtgg aaatatgaca ctcagtgtgg     1260

aaaaagagga ttttgttcgc gcaataacga ttttaaataa taacggcttc ccgaaaaaga     1320

aatttgcaga tatcgaggtt attttcccac ccagccagtt agtggcatcg ccatcacaag     1380

aaaatgcaaa aatcaactat ctcaaagaac aagacatcga aaggttgcta agtaaaatac     1440

ccggtgttat cgattgcagc gtttcattaa atgttaacaa taacgaaagt caaccatcct     1500

cagcagccgt tttagtcatc agctcgccag aggttaactt agcaccatcg gtcattcaga     1560

taaaaaacct ggttaagaat agtgtcgacg atctcaaatt agaaaatatt agtgttgtta     1620

tcaagtcatc ctcaggacag gacgggtaat atgtatgaac aataataatg gcatagcaaa     1680

gaatgattgt gactggctaa cggcccttga ttttgtaaag gatgtcaacg gttcaccaac     1740

gcatctcact ttttatatct accaaaaaaa tgctttcttg cacgattttg gaaactactg     1800

ggttctatat atcgagctta gtggtgattt tcgtcagatt ccaacagata catttatcag     1860

actctgcaat atcctggccg ttagtaaaga atataaacaa atgggtattt tcctgtctaa     1920

taagaagtgg tatctttgcc aaatttttca taaagataat aatcatcgcg caaacatgtc     1980

aaaagcaata atgcaacata ctctcgcctc gttgctcgac aagcaatttg ataaactaga     2040

gcaatcatct tcatcagata caatgatgcc gccaacacac ttgttttctg atataggacg     2100

aattgtgtaa tgaaaaaaat aagttttttt atttttacag cactattttg ctgcagtgca     2160

caagctgccc cgtcctctct ggaaaaaaga ttaggcaaga acgaatattt tattattact     2220

aaaagttctc cagtacgtgc aatccttaat gactttgctg ctaactacag cattcctgtt     2280

ttcatcagta gttccgtgaa tgatgatttc tctggcgaaa taaaaaatga aaaacctgta     2340

aaagtactgg aaaaactgtc caaactctat catctcacat ggtattatga tgagaacatc     2400

ttgtatattt ataaaacaaa tgaaatatca agaagtataa ttacaccaac atatctggat     2460

attgactcac tgctaaaata tctgagtgat acaatctcag taaacaaaaa ctcttgcaat     2520

gtgagaaaaa ttacgacatt taactcgatt gaagttcggg gtgttcctga atgtataaaa     2580

tatattacat ctttatcaga aagtcttgat aaagaggcac agtctaaagc caaaaataaa     2640

gatgttgtta aggtctttaa actgaactat gcgtcagcga cagatataac atacaaatac     2700

cgtgaccaaa atgttgtcgt cccaggtgtt gtctccattc tgaagacaat ggcaagtaat     2760

ggttcgcttc ccagtaccgg taaaggtgcc gtcgagcgaa gcggtaacct gtttgataac     2820

agtgtaacga tttccgctga tccacgtctt aatgcagttg ttgtaaaaga ccgcgaaata     2880

acgatggata tctatcagca attaatttct gagcttgata ttgaacaaag gcaaattgaa     2940

atttctgtct caattattga tgttgatgca aacgatcttc agcagctcgg cgtaaactgg     3000

tccggtacgt taaatgcagg acaaggtacg attgccttta atagctctac cgcacaggct     3060

aatatcagct cttctgtgat aagtaatgcc agtaatttta tgattagggt gaatgcgtta     3120

caacagaact cgaaagctaa aatactctct cagccgtcaa ttataacctt aaataatatg     3180

caggcgattc ttgataaaaa cgtaacgttt tatacaaaag tctccggaga aaaggtggcg     3240

tctctggaaa gtattacaag cggaacattg ctgagagtga caccacgtat acttgatgac     3300

agctcgaata gtcttaccgg aaaacgtcgg gaacgagttc gtttgttact tgatattcag     3360

gatggcaatc aatcgactaa ccaaagcaat gctcaggatg ccagcagcac cttacctgaa     3420

gtacaaaatt cagaaatgac aacagaagcg acattaagtg caggagaaag tttattactt     3480

ggcggattta tccaagataa agaaagttca agtaaagatg gtataccgct gttaagcgac     3540

attcctgtta ttggtagttt attctccagt acggtaaaac aaaaacatag tgttgttcga     3600

ctctttttaa ttaaagcaac cccgataaaa tctgcatcta gcgaataata aatgaggctt     3660

tatgagcagg aaatttagct ctctagagga tatttatgat ttctaccagg atggtggcac     3720

attagcgtca ttaacaaatc tgacacaaca agatctcaat gaccttcatt cttatgccta     3780

tacagcatat caatctggtg atgtaataac cgcaagaaat ctattccatt tgctcacata     3840

tctggaacac tggaattatg actacacctt atctctgggc ttatgccatc agcgtttatc     3900

aaatcatgaa gatgcacaac tgtgtttcgc acgctgtgca actttagtta tgcaagatcc     3960

cagggcatct tattattctg gaattagcta cttactcgtc ggaaataaga aaatggccaa     4020

gaaagccttt aaggcttgtt taatgtggtg taatgaaaaa gaaaaataca ctacatataa     4080

agaaaatatt aaaaaattgt taggtaatac agagtaaact agtttttccg ttataatttc     4140

ttaaagagtg ttccattcgg ggcactcttt tttacattcc ctctacagaa ctagcccttc     4200

tctcacagaa aatgaataaa taaaaatgcg gcaccgccag aatcgcgttc gatgtgacta     4260

tgatcatttt cggtgagtac tgctttgatc taaaggctgg ttttctgtga tgattgacca     4320

gcctcagagt attgatgaca ccaccgttca taacagatcg gcattactat gcataagtac     4380

tgtctcatat tctcgccaaa acacttttta atgcctttgc gaattcatcc agacttatat     4440

tgtcaatttt ttgcccaacg acgactgact ccgagacatc ggccattgcg ggccatcgcg     4500

ttttgtaatc agcataaatt gcaacaacag gggtattata ggcacttgcc atatgtaata     4560

ttgaggtgtc aggagttatt gcgatataag catcttttat gattgctgct gaacgtttaa     4620

tcg                                                                   4623


<210> 3
<211> 6485
<212> DNA
<213> Artificial Sequence


<220> 
<223> Sequence of eLEE operon 3

<400> 3
cttgggctga acgaaccggg agaaagcctg caaccggcct gccatatcag tcaacttgat       60

gccagaacac agcaacatga gatgttaaaa accgcgggtc gccccgtgac tcgtgggatc      120

accttaggcc tgaaggatat tctcaatgcc cgcgaagttt tgttactggt gactggcgaa      180

ggaaagcagg atgcgacaga tcgttttctc acggctaaag tctctaccgc tatcccggct      240

tcatttttat ggctgcacag taactttatt tgtttaatta acacctaacc agatgaaaaa      300

tttgctcaaa atgtttaaat taacttatgt aacagtcacg cattatatta aataacattt      360

gactgggttg aacataacgc cgatagcaaa aggagttatg ttcaacaatt taattttaga      420

tatacatata acttgttcta ttaataaatc acctcgtatt ctgactcgcc ccctggcgca      480

gcaaagtcag gcatttatac tctgagctct tctgaaatga gctgttgaca attaatcatg      540

ggctcgtata atgtgtggaa ttgtgagcgg ataacaattc tagagtcgac aagaaggaga      600

tatacatatg aatcttttag ttaaaagaaa tgttgaagag tttttaagat tgttgggaaa      660

tgatttttat ttatttgata accgtgttga aattgatttt aatgggtttt ctttttttat      720

tgaaataatt gataataatg tttttgttac gtttgcttta gagtataacg aaaacgcatt      780

tttctctttc tttagtgccc ttgctcctga gcgtacgcag ggagtgattg aacatatttt      840

tgtctacgat aataaattgt gcttaagttg tttgttaacc aatatcgatg ttttttttct      900

aatgaatact tttcaacagc atgtgcagat tattgagcgc gttcgcagga tgacatcatg      960

aataaactct taaatatatt taaaaaagca gagtcatatc acgatcttat tctggctctc     1020

ttcttcttta tggctgtaat gatgatgatt attccactac caacagttgt ggtggatatc     1080

attatcgcga ttaatatttc gacagcttta cttttattaa tgctctcaat ctatataaaa     1140

aatcctcttg aactaacttc tttccccaca atcctgttga ttacgacgtt gatgcgcctg     1200

tcgctcagtg ttagtacaac cagacttatt ctgctgcatc acgatgcggg tgacattatt     1260

tactccttcg gtaactttgt tgttggcggt aatattgttg ttggcctggt tatttttact     1320

attatcacca ttgttcaatt tatggttatt acaaaagggg ccgagcgtgt tgctgaagtg     1380

agtgctcgtt tttcccttga tggtatgcca ggaaagcaaa tgagtattga tggtgatatg     1440

cgcgcaggcg ttattgaccc gcttgaggca aaagttcttc gctcccgagt gcaaaaggaa     1500

agccagtttt atggttcaat ggacggggcg atgaagtttg taaaagggga cgctattgct     1560

ggtatcatta ttgttttagt taaccttttt ggtggcgtgc tcattggtat gtggcaattt     1620

gacatgccat ttagtgaggc acttagcctg ttttctgtat tgtctgtcgg tgatgctttg     1680

gttgcccaga tccctgcgct tattatttct gtcaccgcgg gcgtggttgt tactcgtgtg     1740

cccggtgaaa gcgaaaaaga agaaaacctt gcaggtgata ttgttcaaca ggtttctgtt     1800

aatagtcgcc cttttttaat cagtgccgca ctaatgctcg taatggcgat tattccgggc     1860

tttcctacat tggtcttctt atttctggcg gtttgcctgt tggggatagc ctggaaatta     1920

cagaagaaaa gaacatttgg agctggcaat aataaggatg ctatgggagc tgatttgtct     1980

aatagccaaa acatctcacc gggcgctgag ccattgattt taaacttaag tagtaatatt     2040

tatagttcag acattacaca gcaaattgag gtcatgcgtt ggaatttctt tgaggaaagt     2100

ggaattccat tgcctaagat tattgttaat ccggttaaaa ataatgatag cgcaatagaa     2160

tttttgctct atcaagagtc aatatacaaa gatactctta tagatgatac tgtctatttt     2220

gaggctgggc atgcagagat atcattcgaa tttgtccagg aaaagctttc gactaactct     2280

atcgtatata agacgaataa gactaatcaa caactcgctc accttacagg tatggatgtt     2340

tatgcaacaa caaatgataa gataacgttt ttgctaaaga aacttgtatt gtctaatgcc     2400

aaagagttca tcggcgtaca agaaacgcgt tatttgatgg acatcatgga gagaaaatat     2460

aacgagcttg tgaaagagct gcagcgccag cttggtttga gcaaaattgt tgacattcta     2520

caacgtctcg tagaggaaaa tgtctcaatt agagatctga gaactatctt tgagacgctt     2580

attttttggt caacaaaaga aaaggatgtg gttatcttgt gcgaatacgt tcgtatcgcc     2640

ctgcgtcggc atattttagg tcgctatagc gttagcggta cacttctgaa cgtttggctt     2700

attggctctg atattgaaaa tgagctacga gagtcgatca gacaaacgtc atcaggttcg     2760

tatctgaata tctcaccgga gcgaactgag cagataattg gcttcttaaa aaatatcatg     2820

aatccaacgg ggaacggcgt cattctgacc gctttagata tcaggcgcta tgtgaagaaa     2880

atgattgaag gttcgttccc gtcagtcccc gtgctctctt ttcaggaggt tgggaataat     2940

atcgaactta aagtattagg aacggtaaat gatttcagag catgattctg tattggaaaa     3000

atacccacgt attcagaaag tgctcaatag cactgtgccg gcattatcat taaattcgtc     3060

taccagatat gaaggcaaga ttatcaatat cggcgggacg attattaagg cgcgtttacc     3120

aaaagcgcgt attggcgcct tttacaagat agaacccagt caacgtttag ccgaggtgat     3180

cgctattgat gaagatgagg ttttcttgtt gccttttgag catgtttccg gcatgtactg     3240

tggtcagtgg ttaagttatc agggggatga gttcaagatt cgtgttggag acgcactact     3300

tgggcggctt attgatggca taggcagacc aatggagagt aatattgttg ccccctatct     3360

tccgtttgaa cgcagcttgt atgctgaacc accagatcct ttattaaggc aggttattga     3420

tcagccattt atacttggcg tcagggcaat tgatgggcta ctaacttgcg gtattgggca     3480

gcgtatcggt atttttgctg gttcaggcgt tggtaaaagt acgcttttgg ggatgatttg     3540

taatggcgca tccgcagata ttattgtcct tgctcttatc ggcgaacgtg gtcgcgaagt     3600

aaatgaattc ctcgcgcttt tacctcaatc cacgctttct aaatgtgtgc tggtcgtcac     3660

aacgtcagac cgccccgcgc tggaaaggat gaaagccgca tttacggcga cgactattgc     3720

agagtacttc cgcgatcaag gtaaaaatgt attattaatg atggattctg taactcgtta     3780

tgcccgagcc gcgcgtgatg ttgggcttgc atcgggagag cctgatgtaa gggggggatt     3840

ccctccgagt gttttttctt cgttacccaa attattagag cgagcggggc ctgcgccaaa     3900

aggttcaata accgcgattt atacggtgtt gttagaaagc gataatgtta atgatcctat     3960

tggcgatgaa gtccgctcta ttcttgatgg gcacatcgta cttacccgag aacttgcaga     4020

ggaaaaccat ttccctgcaa ttgatattgg tttaagtgcc agtcgtgtta tgcataacgt     4080

tgttacatcg gagcatttgc gtgcggcggc agaatgcaaa aagcttattg caacttataa     4140

aaatgttgag ctgcttattc gtattggtga gtacacgatg gggcaagatc ctgaagcaga     4200

taaggcaata aaaaatagga aattaattca gaactttata caacaaagca ccaaagatat     4260

cagtagttac gaaaaaacga ttgaaagcct attcaaagtg gttgcctgag tatgttggac     4320

agaattttat ctattcgtaa aagcagagcg aaccgattga gagaatcaat ggctaagatt     4380

aactctcaga taaaagaggt ggatggtaaa ctcgatgact gcgagcaatc cattaaagag     4440

tcgattgcct ctaagcaggc gtattgtgca tcgctggtga atttggataa agtgtcgttg     4500

tataaatatc agattaaaaa caatgcattt gatgagcaaa agcagcgatt gtatgagaaa     4560

aaatcttcac tttcgaagga aaagcgttca ttgctagatt cacaaaaacg aacaaaagag     4620

aatctgcaac atgttaataa atcagtagaa aaattatctt ttgccataaa ggaacattac     4680

tttgactaga gtttctctaa aaagaaattt gatagagcct gtttcttttc ggccatcaga     4740

tgattggccg caaggagaag aaagttttaa tgatattttt tatcatataa aaaaaagatc     4800

gcctgatacg ttgttcatac gaatggcttc tttatggaag agattgtttt actcctcggg     4860

ccgaagacga cgctactttg aagaagggga gcactcgttt agtattcttt gtggaagatt     4920

acgaggagtt gttttaacaa ttaaatgtag taacggaatc atttatctgt ctattaaaat     4980

aagccaaaat aatagaaatc atgttttttt atatcataaa aaagactatg ttttcgataa     5040

gttaaaagaa atctttcctg atgaagccat tgagttcaca attgaatatg aaaattaacg     5100

atttttattt gccgttgttg ccagttatcg gaaccggtcg cctctatatt acgtccgaag     5160

gtcatgcttg ccatgcatat tttcgtgagg tcagcggtca tggtattcgt ttcacgttga     5220

catactcggg ctatgagggg cgtttctgga tatcagaaga acagttcatt cagtggtgcc     5280

aagagttatt cccgtatagt gaaagccggc tgataccgga agatatgata aaactcatga     5340

tcttatgggt aatgcagact gccttaccgg aaggtgatgt gtcagttgat gatgttcaat     5400

ttacgatgct aaataaagat gtttatccgg taatagaaaa taataatgga gaaaacagac     5460

ttaatgtaat tatactcgaa acaacggtac aaagtttaca acatctgatt aatgataatt     5520

ggcaatttgt tccacattct aatgctcttt tttttgatgg ctatatcgta cctgggtgga     5580

ctgattatcc agttacagag ttgagagttg gtgatagtct gagactctat catgttgatg     5640

atagtcaaga gagaaggtgc tggattgtta ttaataaccc tttggcaacc gtaaagctga     5700

gcgataataa tttatctgtc acggatgttc tggctgcaga tccgttatgt actatcagca     5760

gcgagactgt gatgaatcgc atttattgtg tgattggcac agtgcatatt gatatacata     5820

tgttgcgtaa tgtccaaaaa gatgacatta tcgattcaac gggatatcat ctttttggtg     5880

ggtgccagtt agtacggaat aataccacaa tcgcatacgg gagtattata aaaatcaacg     5940

aagattttta ttttactatt agcgtagtat gtgattaaac tagtcatggc accgtcaata     6000

tgcgcgcctg gccagttaat ctggtcggtg gtgagctgga aacaggaaaa tttcagggca     6060

cagccaccat taccgtcatc gtgcggtaaa agaagacgtt gcgttttgat taacgcaacg     6120

cttcggcact gttacccctg ctgctccagc gcatacttat acagcgcatt tttcttcacg     6180

ccgtgaattt ctgcggccag cgccgccgct tttttcagcg gcagttctgc ctgtagcagc     6240

gccagcgtgc gcagggcatc ggcgggtaag tcttcttcct gtgctttatg accttcgaca     6300

atcagcacca tttcgccttt gcgacggttt tcatcttcct ttacccacgc cagcagctcg     6360

ccaacgggcg cgccgtgaat ggtttcccag gttttggtca gctcacgcgc cagaaccacg     6420

tagcgggatt cgcctaatac cgcaacgata tcttccaggc tatctaacag acggtgggta     6480

gattc                                                                 6485


<210> 4
<211> 5898
<212> DNA
<213> Artificial Sequence


<220> 
<223> Sequence of eLEE operon 4

<400> 4
cgctgttagc gtcaatcgtt ttattgttac ctgccaacct gttgcctatt tctatcattt       60

atctgaacgg aggacggcag gaagatacaa ttctttccgg aattatgtcg ctggcaagta      120

gcaacattgc ggttgcagga atcgtgttta tcgccagtat tctggtaccg tttactaaag      180

tgatcgtcat gttcacttta ctgttgagca ttcattttaa atgccagcaa ggtttacgca      240

cacgcattct gttactgcgg atggtgacct ggattggtcg ctggtcgatg ctcgacctgt      300

ttgtcatatc tttaaccatg tcgctgatta atcgcgatca gatcctcgct tttactatgg      360

gaccggctgc gttttatttc ggcgcagcgg taattttgac tattcttgct gtggaatggc      420

tggacagccg cttactttgg gatgcacatg agtcaggaaa cgcccgcttc gacgactgaa      480

gcgcagatta aaaataaacg gagctcttct gaaatgagct gttgacaatt aatcatgggc      540

tcgtataatg tgtggaattg tgagcggata acaattctag agtcgacaag aaggagatat      600

acatatggct aatggtattg aatttaatca aaaccccgca tctgttttta attctaattc      660

attagatttt gaattagaat ctcagcaatt aacgcaaaaa aattcttcta atacttcttc      720

gccattaata aatttgcaaa atgaattagc aatgattact agctcatcgc tttctgaaac      780

gattgaaggg ctaagcctgg gatatcgcaa aggtagcgca aggaaagaag aggaaggcac      840

gacaatcgag aagctgctca atgaaatgca agagctgctt actcttaccg atagtgataa      900

aataaaagaa ctatcattaa aaaacagcgg gttgctggaa caacacgatc ctactttggc      960

gatgtttggc aacatgccaa agggggaaat tgttgcgcta atatcttctt tattacaatc     1020

taaattcgtt aagattgaat taaaaaagaa atacgccaaa ttattattgg atttattagg     1080

cgaagacgat tgggagctgg ctctgctttc ctggttaggg gtgggtgagt taaaccagga     1140

aggtatccag aagatcaaga agctttatga aaaggctaag gatgaggatt ctgaaaatgg     1200

cgcctcttta cttgactggt ttatggagat taaggatctt cctgagcgtg aaaagcactt     1260

aaaggttatt attagggcgc tatcgttcga tctgtcttat atgtcttctt ttgaagacaa     1320

ggtaagaaca tcttcaatta ttagtgattt atgtcggata atcatttttt tatcacttaa     1380

taactatacg gatattattg caatctccat taagaaagat aaagatgtca ttttaaatga     1440

aatgctatct attattgaac atgtatggtt aacagaagac tggctactgg agagcccttc     1500

tcgggtatcg attgtcgaag ataaacatgt ttattatttc catttattga aagaattttt     1560

tgcatcatta cctgatgctt gttttattga taacgagcag aggagtaata cattattaat     1620

gattggtaaa gtgatagatt ataaggagga tgttatgtga tattgttttt tttatagttt     1680

ttgtcatgct aagaaagatt atgaagaggt atatacatgg atacatcaac tacagcatca     1740

gttgctagtg cgaatgcgag tacttcgaca tcaatggcct atgatttagg gagcatgtcg     1800

aaagatgacg ttattgatct atttaataaa ctcggtgttt ttcaggctgc aattctcatg     1860

tttgcctata tgtatcaggc acaaagcgat ctgtcgattg caaagtttgc tgatatgaat     1920

gaggcatcta aggagtcaac cactgcccaa aaaatggcta atcttgtaga tgctaaaatt     1980

gctgacgttc agagtagctc tgacaagaat gcgaaagctc aacttcctga tgaagtgatt     2040

tcatatataa atgatcctcg caatgacatt acaataagtg gtattgacaa tataaatgct     2100

caattaggcg ctggtgattt gcaaacggtg aaagcagcta tttcagctaa agcgaataat     2160

ttgacaacga cggtcaataa tagccagctt gaaatacagc aaatgtcaaa tacgttaaac     2220

ctattaacga gtgcacgttc tgatatgcag tcactgcaat atagaactat ttcaggaata     2280

tcccttggta aataaccgga gataactatg cttaatgtaa ataacgatat ccagtctgtg     2340

aggtctggag ccagtgctgc tacggctact tcaggtatta atcagtctga agtgacttcc     2400

gcgctggatt tacaactggt taaatccacg gccccatcag caagttggac agaaagtacc     2460

gctttaccaa cgccaccggc aggtcactca ttagtgacgc cctctgctgc tgaggatgtc     2520

ctgagtaagc tgtttggtgg tattagtggt gaagttacga gtcgaacaga gggaacagaa     2580

ccacagcgca gcactcagaa tgcaagctct ggctatcctt atctttctca ggtgaataac     2640

gttgaccctc aggccatgat gatgatggcc actctgttat ccctggatgc ctcagcgcag     2700

agagttgcga gtatgaaaaa ctccaacgag atctacgcgg atggacaaaa taaagcgctg     2760

gataataaaa cgctggagtt taaaaagcag ctcgaagaac aacaaaaagc cgaagagaaa     2820

gcacaaaaaa gtaaaattgt tggtcaggtc tttggttggt taggggttgc agcaactgct     2880

attgcagcta tttttaaccc ggcactctgg gctgttgttg ctattagtgc aacagcaatg     2940

gcactgcaaa ctgcagttga tgttatgggc gatgatgctc cacaggcgtt aaaaacagca     3000

gcgcaagctt ttggcggtct atctcttgcc gcaggtattc tgacagccgg cattggcggg     3060

gtgtcttcac ttatatctaa agttggcgat gtcgctaaca aagttggttc aaacatcgta     3120

aaagtagtca ctacgctggc cgacactttt gttgataatg ttgcatcgaa aatttcggca     3180

gtggcaaatg gtttaactac ttcttcgcgc tctattggta caactgtgct gaataatgac     3240

gcagcgtatt ataatgtgtt atcacaggtt tctgcttttg ctgtggaaaa tttaactcga     3300

caaagtgagt acttaagtca gagtgcgaag gcagagctgg aaaaagctac tctcgagctg     3360

caaaaccagg cgaattatat acagagtgct tctcagctga tgtctgattc tgcgcgagtg     3420

aatatccgta ttgtcagcgg tcgagtttaa ttattaaaga ggcgtttatt atgaatacta     3480

tcgataataa caatgcggca atcgcagtta attctgtttt gagcagcacg actgacagca     3540

ccagttcaac aactacatca acttcgtcaa tcagttcatc tttacttact gatggcagag     3600

tggatatttc taaacttctg ctggaggttc aaaaactgtt gcgtgagatg gtcaccacat     3660

tgcaagatta tcttcagaag caattggcgc aaagctatga catccaaaaa gccgtttttg     3720

agagccaaaa taaagctatt gatgagaaaa aagccggtgc gacagccgct ctgattggtg     3780

gtgctatctc atctgtcctg gggattttag gctcttttgc tgccattaat agcgcaacta     3840

aaggcgcgag tgatgtcgct cagcaagccg cttctacttc tgcgaagtct atcggtacag     3900

tctctgaagc ttcaactaaa gcactggcga aggcttccga aggtattgca gatgcagcag     3960

atgatgcagc tggcgcaatg cagcaaacta tcgcgacagc tgcaaaagcg gccagtcgta     4020

catccggtat cactgatgat gttgctactt cggctcagaa agcttctcag gtagctgaag     4080

aggctgctga tgctgctcaa gaattagcac agaaggcagg attattaagt cgctttactg     4140

ctgctgccgg aaggatttcc ggttcaacgc catttattgt tgttaccagc cttgctgaag     4200

gtacgaaaac attgccaaca acgatatctg aatctgtcaa gtccaatcat gatatcaatg     4260

agcagcgtgc taaatctgta gaaaatcttc aggcgtcgaa tttagatacc tacaaacagg     4320

atgttcgcag agcgcaggat gatatctcta gccgtctacg tgatatgaca acgacagccc     4380

gtgatcttac tgaccttatt aatcgcatgg ggcaagcggc tcgcttagct gggtaattga     4440

tcatggtcga tacgtttaat gatgaagtgt ttaattacta tcttgaacaa aaagggtaca     4500

caatacagaa ggagtttctt tgtggcagtg ccttttttat cggatggcgg attgagacac     4560

cttttttttc attagcgtac agactggatg aacaagaact gattttgtgc tcttttgaag     4620

cacgtaacca aacagggctt aacggccctg ttttatcact gactcgtttg ctcgaagagt     4680

tgtatcacca cttttcgggt attaagaaaa tcagtgcgat gaaatctaag attggttcag     4740

attcagaacg tcaaaagcgc gaagagttgt ttaattactt catcagaaag ggtgccgtgc     4800

agcaagaaac agaagacgga atttggttcg taatgaacgt aaatagttaa aaaaaaatta     4860

ttttattaac ttctgaggga aatttaatga atttatctga aattactcaa caaatgggtg     4920

aagtaggtaa aacgctgagc gattctgtgc cagagttact taatagcacc gatttggtta     4980

atgaccctga aaaaatgtta gagttgcagt ttgcggttca gcaatattct gcttatgtta     5040

acgtagaaag tggaatgttg aaaacgataa aagatctggt ctcaaccatt tctaaccgta     5100

gtttttaatt tttatggtta atgatatttc tgctaataag atactggtgt gggcggctgt     5160

agctgcggca aaccataagc ttcccaaata tgcggaagct atcctgaatg tattcccaca     5220

aattatacct gataaaaaag atatcgcaca tttagaattt attatcttat ttggattaaa     5280

tagaaaaaat gatgcgataa aggctttgga gggctgtatg gatgatgagt cgagtcagtt     5340

attgtacagt ttgttccatg agaatgttag tggttgggta cgaggatttt aaactagtgg     5400

tacgccactg gcaccgaaag cccaggaagg caaacacttc ctgttgcagg aaagtgaacc     5460

gaaagagtgg cgtgaatggg gaactgcgct tcccaaataa tgcccactgc tccggcgtgc     5520

ctgcgccgga gcgtttatgc taaactgcgc gcctgttttt ttgccagtgg tacatgctcg     5580

tggcccaaca caccgtttat ttcccggacg cctttctgac acaaatgcgc gaagcgatgc     5640

cttcgacgct ctcatttgat gattttcttg ccgcctgtca gcgcccgttg cgccgcagca     5700

ttcgcgttaa tacgctgaaa atctccgttg ctgatttcct gcaattaacc gctccttatg     5760

gctggacgct tacgccaatt ccgtggtgtg aagaaggttt ctggattgaa cgcgacaatg     5820

aagatgcatt gccattgggt agtaccgccg agcatttaag tggcctgttt tatattcagg     5880

aagccagttc aatgttgc                                                   5898


<210> 5
<211> 3434
<212> DNA
<213> Artificial Sequence


<220> 
<223> >Sequence of eLEE operon 5 (EscD)

<400> 5
ggcagcggat atctgtcaac ctcactacta tcgaagactc tccccatcta taaataaaac       60

acctttaact ttcattcgga aagtattctt ctgcgcgtat cttgattttg cccaaagtta      120

catgttgaaa tgaataatgt ctgtttgaca ctcattatca tttattaata cagataatgg      180

agaaaataga gttgaataat caggcaggac gtaattgaaa cttgattaaa ttctggctat      240

agtgtttata tattatatcg acaaaagcgg gttaatgcca atcgcattat atgttcattg      300

tttgtttttt aagctattgg cattcattgt tgatatgctt cctgtttttt ctaaatatat      360

atttcgtgtt tttttagaag atccttaaga taatctttcc cctatcaata tattaattaa      420

atctacattt actcattagt aaaattaaat tgaatcaggg gagtgaaaac atcactattc      480

gaccccacac caacgaatac gagctcttct gaaatgagct gttgacaatt aatcatgggc      540

tcgtataatg tgtggaattg tgagcggata acaattctag agtcgacaag aaggagatat      600

acatatgtta tcctcatata aaataaaact tctcaatggt gcgatgcgta atcgggaatt      660

acaacttccg atgggaaacc taacgctagg tacggaagat aacgatattg tttactttcc      720

tcttgaacaa ggcctcaatc agtttttact tgatatacgt gaggaaggtg ttttcctttt      780

atctccggtt gaattttgga ttgatgggca acccacgccc tatgaagcag ataaaccatt      840

acctgtcgga aagattattg atatagcggg atgttgcttc atcattggtg atattgacca      900

ttccttgcca ttgtctgatg tgccagaaag gttttctgcc aaaaatcaac gcaaaaaacg      960

ccttatcctg gcgagcgtga ttggtgctac ttttgcgttg tctggtgcca tcggcagtta     1020

cgttttgctt tccccaaaag ctgaaccccc aacgtttacc agagctgatg tatatcagca     1080

gctaaaagaa aacaaacttc atgcaatcac tctggtatgg catggaaaaa atatcgcgct     1140

ttatgggcgt tgcgaatcaa ccacggattt aactccattt tttaattacc tgaaagaaaa     1200

aaatattttt tattacaata aaatcatttg caataaccaa atcatatccg caattaatga     1260

tgtcttaacg gaatacggtt acaaagatat cattattaca aaaggtaata agcctgggtt     1320

ctttctgtta tcgggataca ttcccccttc acccaaatgg agcgaggttg aaaatctgtt     1380

actgaatacg ccaggggttg caggatggga aatacacaac aacagtaaca ataaaatcaa     1440

tgaactggca tcagagttta aaaagaataa actaataaac tatgtcaata ttttcaaaaa     1500

gaatgatgtc atcattgtcg cgggggaagt ttctcaacag aacgagagta agatccttgc     1560

cattataaat gcaatgaaca aaaatagtaa cgtcaagata ctctttcaga acattcagcc     1620

gtacatttca gcagatattt ttcccggaaa aatactcaga atcagcggta cgatgaagaa     1680

tcctacaatt gctcttgata atggtaccag cctcgggatt ggtagcattc tgaaaggagg     1740

ctatgtcatc gacgcaattg atcctaaaga tggtatcaac atctcccgcc cagacgaata     1800

tatacatatt ccactgtcgt attaaactag tgaagttcct atactttcta gagaatagga     1860

acttcggaat aggaacttca tgagtaaact tggtctgaca gttaccaatg cttaatcagt     1920

gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc     1980

gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg     2040

cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc     2100

gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg     2160

gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca     2220

ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga     2280

tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct     2340

ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg     2400

cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca     2460

accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata     2520

cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct     2580

tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact     2640

cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa     2700

acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc     2760

atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga     2820

tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga     2880

gaagttccta tactttctag agaataggaa cttcggaata ggaacttcac tagtggacaa     2940

acagttacac gatatggtcg tgcgtgttga taccagcagt atgcagccaa tcgattctac     3000

ttgccggttc cagtacatac tgaatgagtt gtagccctta gatacaccag gcaggggcat     3060

tcgcccctgc cattacccgt acctggcgca acgcgctcaa ctcctcaggg caactccggc     3120

ggcagccact cttcgacttc aatcaacacc aacaacgccg catcaccgcc atactctttt     3180

ggtgcctgat gaaaggccat cacatgcgga tgttgtgcca gccacagcgg tgtttgttgc     3240

ttcaaaatat gcttcccgtg cccatgcatc acgcaggcgc aaaacacatg ttcacggcgg     3300

caggcggcaa tcaacgcccc cagttcctgc ttggcctgca gctgcgtcag accgtgtaaa     3360

tccaaaaaca actccggcga ataatcgcca cggcgcagtt tcttcgcctc aaaatggctg     3420

acatccgggc gaac                                                       3434


<210> 6
<211> 69
<212> DNA
<213> Artificial Sequence


<220> 
<223> >tac promoter

<400> 6
ttctgaaatg agctgttgac aattaatcat gggctcgtat aatgtgtgga attgtgagcg     60

gataacaat                                                             69


<210> 7
<211> 30
<212> PRT
<213> Artificial Sequence


<220> 
<223> >Tir30

<400> 7
Met Pro Ile Gly Asn Leu Gly Asn Asn Val Asn Gly Asn His Leu Ile 
1               5                   10                  15      
Pro Pro Ala Pro Pro Leu Pro Ser Gln Thr Asp Gly Ala Ala 
            20                  25                  30  

<210> 8
<211> 100
<212> PRT
<213> Artificial Sequence


<220> 
<223> Tir100

<400> 8
Met Pro Ile Gly Asn Leu Gly Asn Asn Val Asn Gly Asn His Leu Ile 
1               5                   10                  15      
Pro Pro Ala Pro Pro Leu Pro Ser Gln Thr Asp Gly Ala Ala Arg Gly 
            20                  25                  30          
Gly Thr Gly His Leu Ile Ser Ser Thr Gly Ala Leu Gly Ser Arg Ser 
        35                  40                  45              
Leu Phe Ser Pro Leu Arg Asn Ser Met Ala Asp Ser Val Asp Ser Arg 
    50                  55                  60                  
Asp Ile Pro Gly Leu Pro Thr Asn Pro Ser Arg Leu Ala Ala Ala Thr 
65                  70                  75                  80  
Ser Glu Thr Cys Leu Leu Gly Gly Phe Glu Val Leu His Asp Lys Gly 
                85                  90                  95      
Pro Leu Asp Ile 
            100 

<210> 9
<211> 20
<212> PRT
<213> Artificial Sequence


<220> 
<223> >EspF20

<400> 9
Met Leu Asn Gly Ile Ser Asn Ala Ala Ser Thr Leu Gly Arg Gln Leu 
1               5                   10                  15      
Val Gly Ile Ala 
            20  

<210> 10
<211> 156
<212> PRT
<213> Escherichia coli


<220> 
<223> >CesT sequence protein

<400> 10
Met Ser Ser Arg Ser Glu Leu Leu Leu Asp Arg Phe Ala Glu Lys Ile 
1               5                   10                  15      
Gly Val Gly Ser Ile Ser Phe Asn Glu Asn Arg Leu Cys Ser Phe Ala 
            20                  25                  30          
Ile Asp Glu Ile Tyr Tyr Ile Ser Leu Ser Asp Ala Asn Asp Glu Tyr 
        35                  40                  45              
Met Met Ile Tyr Gly Val Cys Gly Lys Phe Pro Thr Asp Asn Pro Asn 
    50                  55                  60                  
Phe Ala Leu Glu Ile Leu Asn Ala Asn Leu Trp Phe Ala Glu Asn Gly 
65                  70                  75                  80  
Gly Pro Tyr Leu Cys Tyr Glu Ser Gly Ala Gln Ser Leu Leu Leu Ala 
                85                  90                  95      
Leu Arg Phe Pro Leu Asp Asp Ala Thr Pro Glu Lys Leu Glu Asn Glu 
            100                 105                 110         
Ile Glu Val Val Val Lys Ser Met Glu Asn Leu Tyr Leu Val Leu His 
        115                 120                 125             
Asn Gln Gly Ile Thr Leu Glu Asn Glu His Met Lys Ile Glu Glu Ile 
    130                 135                 140                 
Ser Ser Ser Asp Asn Lys His Tyr Tyr Ala Gly Arg 
145                 150                 155     

<210> 11
<211> 360
<212> PRT
<213> Escherichia coli


<220> 
<223> > lacI protein

<400> 11
Met Lys Pro Val Thr Leu Tyr Asp Val Ala Glu Tyr Ala Gly Val Ser 
1               5                   10                  15      
Tyr Gln Thr Val Ser Arg Val Val Asn Gln Ala Ser His Val Ser Ala 
            20                  25                  30          
Lys Thr Arg Glu Lys Val Glu Ala Ala Met Ala Glu Leu Asn Tyr Ile 
        35                  40                  45              
Pro Asn Arg Val Ala Gln Gln Leu Ala Gly Lys Gln Ser Leu Leu Ile 
    50                  55                  60                  
Gly Val Ala Thr Ser Ser Leu Ala Leu His Ala Pro Ser Gln Ile Val 
65                  70                  75                  80  
Ala Ala Ile Lys Ser Arg Ala Asp Gln Leu Gly Ala Ser Val Val Val 
                85                  90                  95      
Ser Met Val Glu Arg Ser Gly Val Glu Ala Cys Lys Ala Ala Val His 
            100                 105                 110         
Asn Leu Leu Ala Gln Arg Val Ser Gly Leu Ile Ile Asn Tyr Pro Leu 
        115                 120                 125             
Asp Asp Gln Asp Ala Ile Ala Val Glu Ala Ala Cys Thr Asn Val Pro 
    130                 135                 140                 
Ala Leu Phe Leu Asp Val Ser Asp Gln Thr Pro Ile Asn Ser Ile Ile 
145                 150                 155                 160 
Phe Ser His Glu Asp Gly Thr Arg Leu Gly Val Glu His Leu Val Ala 
                165                 170                 175     
Leu Gly His Gln Gln Ile Ala Leu Leu Ala Gly Pro Leu Ser Ser Val 
            180                 185                 190         
Ser Ala Arg Leu Arg Leu Ala Gly Trp His Lys Tyr Leu Thr Arg Asn 
        195                 200                 205             
Gln Ile Gln Pro Ile Ala Glu Arg Glu Gly Asp Trp Ser Ala Met Ser 
    210                 215                 220                 
Gly Phe Gln Gln Thr Met Gln Met Leu Asn Glu Gly Ile Val Pro Thr 
225                 230                 235                 240 
Ala Met Leu Val Ala Asn Asp Gln Met Ala Leu Gly Ala Met Arg Ala 
                245                 250                 255     
Ile Thr Glu Ser Gly Leu Arg Val Gly Ala Asp Ile Ser Val Val Gly 
            260                 265                 270         
Tyr Asp Asp Thr Glu Asp Ser Ser Cys Tyr Ile Pro Pro Leu Thr Thr 
        275                 280                 285             
Ile Lys Gln Asp Phe Arg Leu Leu Gly Gln Thr Ser Val Asp Arg Leu 
    290                 295                 300                 
Leu Gln Leu Ser Gln Gly Gln Ala Val Lys Gly Asn Gln Leu Leu Pro 
305                 310                 315                 320 
Val Ser Leu Val Lys Arg Lys Thr Thr Leu Ala Pro Asn Thr Gln Thr 
                325                 330                 335     
Ala Ser Pro Arg Ala Leu Ala Asp Ser Leu Met Gln Leu Ala Arg Gln 
            340                 345                 350         
Val Ser Arg Leu Glu Ser Gly Gln 
        355                 360 

<210> 12
<211> 360
<212> PRT
<213> Artificial Sequence


<220> 
<223> > lacI W220F protein

<400> 12
Met Lys Pro Val Thr Leu Tyr Asp Val Ala Glu Tyr Ala Gly Val Ser 
1               5                   10                  15      
Tyr Gln Thr Val Ser Arg Val Val Asn Gln Ala Ser His Val Ser Ala 
            20                  25                  30          
Lys Thr Arg Glu Lys Val Glu Ala Ala Met Ala Glu Leu Asn Tyr Ile 
        35                  40                  45              
Pro Asn Arg Val Ala Gln Gln Leu Ala Gly Lys Gln Ser Leu Leu Ile 
    50                  55                  60                  
Gly Val Ala Thr Ser Ser Leu Ala Leu His Ala Pro Ser Gln Ile Val 
65                  70                  75                  80  
Ala Ala Ile Lys Ser Arg Ala Asp Gln Leu Gly Ala Ser Val Val Val 
                85                  90                  95      
Ser Met Val Glu Arg Ser Gly Val Glu Ala Cys Lys Ala Ala Val His 
            100                 105                 110         
Asn Leu Leu Ala Gln Arg Val Ser Gly Leu Ile Ile Asn Tyr Pro Leu 
        115                 120                 125             
Asp Asp Gln Asp Ala Ile Ala Val Glu Ala Ala Cys Thr Asn Val Pro 
    130                 135                 140                 
Ala Leu Phe Leu Asp Val Ser Asp Gln Thr Pro Ile Asn Ser Ile Ile 
145                 150                 155                 160 
Phe Ser His Glu Asp Gly Thr Arg Leu Gly Val Glu His Leu Val Ala 
                165                 170                 175     
Leu Gly His Gln Gln Ile Ala Leu Leu Ala Gly Pro Leu Ser Ser Val 
            180                 185                 190         
Ser Ala Arg Leu Arg Leu Ala Gly Trp His Lys Tyr Leu Thr Arg Asn 
        195                 200                 205             
Gln Ile Gln Pro Ile Ala Glu Arg Glu Gly Asp Phe Ser Ala Met Ser 
    210                 215                 220                 
Gly Phe Gln Gln Thr Met Gln Met Leu Asn Glu Gly Ile Val Pro Thr 
225                 230                 235                 240 
Ala Met Leu Val Ala Asn Asp Gln Met Ala Leu Gly Ala Met Arg Ala 
                245                 250                 255     
Ile Thr Glu Ser Gly Leu Arg Val Gly Ala Asp Ile Ser Val Val Gly 
            260                 265                 270         
Tyr Asp Asp Thr Glu Asp Ser Ser Cys Tyr Ile Pro Pro Leu Thr Thr 
        275                 280                 285             
Ile Lys Gln Asp Phe Arg Leu Leu Gly Gln Thr Ser Val Asp Arg Leu 
    290                 295                 300                 
Leu Gln Leu Ser Gln Gly Gln Ala Val Lys Gly Asn Gln Leu Leu Pro 
305                 310                 315                 320 
Val Ser Leu Val Lys Arg Lys Thr Thr Leu Ala Pro Asn Thr Gln Thr 
                325                 330                 335     
Ala Ser Pro Arg Ala Leu Ala Asp Ser Leu Met Gln Leu Ala Arg Gln 
            340                 345                 350         
Val Ser Arg Leu Glu Ser Gly Gln 
        355                 360 

<210> 13
<211> 49
<212> DNA
<213> Artificial Sequence


<220> 
<223> > PR promoter

<400> 13
taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgc                 49


<210> 14
<211> 56
<212> DNA
<213> Artificial Sequence


<220> 
<223> >Ptet promoter (complete, bidirectional)

<400> 14
ttctctatca ctgataggga gtggtaaaat aactctatca atgatagagt gtcaac         56


<210> 15
<211> 207
<212> PRT
<213> Escherichia coli


<220> 
<223> > TetR protein

<400> 15
Met Ser Arg Leu Asp Lys Ser Lys Val Ile Asn Ser Ala Leu Glu Leu 
1               5                   10                  15      
Leu Asn Glu Val Gly Ile Glu Gly Leu Thr Thr Arg Lys Leu Ala Gln 
            20                  25                  30          
Lys Leu Gly Val Glu Gln Pro Thr Leu Tyr Trp His Val Lys Asn Lys 
        35                  40                  45              
Arg Ala Leu Leu Asp Ala Leu Ala Ile Glu Met Leu Asp Arg His His 
    50                  55                  60                  
Thr His Phe Cys Pro Leu Glu Gly Glu Ser Trp Gln Asp Phe Leu Arg 
65                  70                  75                  80  
Asn Asn Ala Lys Ser Phe Arg Cys Ala Leu Leu Ser His Arg Asp Gly 
                85                  90                  95      
Ala Lys Val His Leu Gly Thr Arg Pro Thr Glu Lys Gln Tyr Glu Thr 
            100                 105                 110         
Leu Glu Asn Gln Leu Ala Phe Leu Cys Gln Gln Gly Phe Ser Leu Glu 
        115                 120                 125             
Asn Ala Leu Tyr Ala Leu Ser Ala Val Gly His Phe Thr Leu Gly Cys 
    130                 135                 140                 
Val Leu Glu Asp Gln Glu His Gln Val Ala Lys Glu Glu Arg Glu Thr 
145                 150                 155                 160 
Pro Thr Thr Asp Ser Met Pro Pro Leu Leu Arg Gln Ala Ile Glu Leu 
                165                 170                 175     
Phe Asp His Gln Gly Ala Glu Pro Ala Phe Leu Phe Gly Leu Glu Leu 
            180                 185                 190         
Ile Ile Cys Gly Leu Glu Lys Gln Leu Lys Cys Glu Ser Gly Ser 
        195                 200                 205         

<210> 16
<211> 237
<212> PRT
<213> Enterobacteria phage lambda


<220> 
<223> >cl protein

<400> 16
Met Ser Thr Lys Lys Lys Pro Leu Thr Gln Glu Gln Leu Glu Asp Ala 
1               5                   10                  15      
Arg Arg Leu Lys Ala Ile Tyr Glu Lys Lys Lys Asn Glu Leu Gly Leu 
            20                  25                  30          
Ser Gln Glu Ser Val Ala Asp Lys Met Gly Met Gly Gln Ser Gly Val 
        35                  40                  45              
Gly Ala Leu Phe Asn Gly Ile Asn Ala Leu Asn Ala Tyr Asn Ala Ala 
    50                  55                  60                  
Leu Leu Ala Lys Ile Leu Lys Val Ser Val Glu Glu Phe Ser Pro Ser 
65                  70                  75                  80  
Ile Ala Arg Glu Ile Tyr Glu Met Tyr Glu Ala Val Ser Met Gln Pro 
                85                  90                  95      
Ser Leu Arg Ser Glu Tyr Glu Tyr Pro Val Phe Ser His Val Gln Ala 
            100                 105                 110         
Gly Met Phe Ser Pro Glu Leu Arg Thr Phe Thr Lys Gly Asp Ala Glu 
        115                 120                 125             
Arg Trp Val Ser Thr Thr Lys Lys Ala Ser Asp Ser Ala Phe Trp Leu 
    130                 135                 140                 
Glu Val Glu Gly Asn Ser Met Thr Ala Pro Thr Gly Ser Lys Pro Ser 
145                 150                 155                 160 
Phe Pro Asp Gly Met Leu Ile Leu Val Asp Pro Glu Gln Ala Val Glu 
                165                 170                 175     
Pro Gly Asp Phe Cys Ile Ala Arg Leu Gly Gly Asp Glu Phe Thr Phe 
            180                 185                 190         
Lys Lys Leu Ile Arg Asp Ser Gly Gln Val Phe Leu Gln Pro Leu Asn 
        195                 200                 205             
Pro Gln Tyr Pro Met Ile Pro Cys Asn Glu Ser Cys Ser Val Val Gly 
    210                 215                 220                 
Lys Val Ile Ala Ser Gln Trp Pro Glu Glu Thr Phe Gly 
225                 230                 235         

<210> 17
<211> 237
<212> PRT
<213> Enterobacteria phage lambda


<220> 
<223> >cl+ ind- protein

<400> 17
Met Ser Thr Lys Lys Lys Pro Leu Thr Gln Glu Gln Leu Glu Asp Ala 
1               5                   10                  15      
Arg Arg Leu Lys Ala Ile Tyr Glu Lys Lys Lys Asn Glu Leu Gly Leu 
            20                  25                  30          
Ser Gln Glu Ser Val Ala Asp Lys Met Gly Met Gly Gln Ser Gly Val 
        35                  40                  45              
Gly Ala Leu Phe Asn Gly Ile Asn Ala Leu Asn Ala Tyr Asn Ala Ala 
    50                  55                  60                  
Leu Leu Ala Lys Ile Leu Lys Val Ser Val Glu Glu Phe Ser Pro Ser 
65                  70                  75                  80  
Ile Ala Arg Glu Ile Tyr Glu Met Tyr Glu Ala Val Ser Met Gln Pro 
                85                  90                  95      
Ser Leu Arg Ser Glu Tyr Glu Tyr Pro Val Phe Ser His Val Gln Ala 
            100                 105                 110         
Gly Met Phe Ser Pro Lys Leu Arg Thr Phe Thr Lys Gly Asp Ala Glu 
        115                 120                 125             
Arg Trp Val Ser Thr Thr Lys Lys Ala Ser Asp Ser Ala Phe Trp Leu 
    130                 135                 140                 
Glu Val Glu Gly Asn Ser Met Thr Ala Pro Thr Gly Ser Lys Pro Ser 
145                 150                 155                 160 
Phe Pro Asp Gly Met Leu Ile Leu Val Asp Pro Glu Gln Ala Val Glu 
                165                 170                 175     
Pro Gly Asp Phe Cys Ile Ala Arg Leu Gly Gly Asp Glu Phe Thr Phe 
            180                 185                 190         
Lys Lys Leu Ile Arg Asp Ser Gly Gln Val Phe Leu Gln Pro Leu Asn 
        195                 200                 205             
Pro Gln Tyr Pro Met Ile Pro Cys Asn Glu Ser Cys Ser Val Val Gly 
    210                 215                 220                 
Lys Val Ile Ala Ser Gln Trp Pro Glu Glu Thr Phe Gly 
225                 230                 235         

<210> 18
<211> 103
<212> DNA
<213> Artificial Sequence


<220> 
<223> > T0 terminator

<400> 18
cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga     60

acgctcggtt gccgccgggc gttttttatt ggtgagaatc cag                      103


<210> 19
<211> 87
<212> DNA
<213> Artificial Sequence


<220> 
<223> >T1 terminator

<400> 19
atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc     60

tttcgactga gcctttcgtt ttatttg                                         87


<210> 20
<211> 87
<212> DNA
<213> Artificial Sequence


<220> 
<223> >RBS 0030 sequence

<400> 20
atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc     60

tttcgactga gcctttcgtt ttatttg                                         87


<210> 21
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> AAV-ssrA tag

<400> 21
Ala Ala Asn Asp Glu Asn Tyr Ala Ala Ala Val 
1               5                   10      

<210> 22
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> ASV-ssrA tag

<400> 22
Ala Ala Asn Asp Glu Asn Tyr Ala Ala Ala Val 
1               5                   10      

<210> 23
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> LVA-ssrA tag

<400> 23
Ala Ala Asn Asp Glu Asn Tyr Ala Leu Val Ala 
1               5                   10      

<210> 24
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> LAA-ssrA tag

<400> 24
Ala Ala Asn Asp Glu Asn Tyr Ala Leu Ala Ala 
1               5                   10      

<210> 25
<211> 825
<212> PRT
<213> Artificial Sequence


<220> 
<223> Synthetic adhesin full

<400> 25
Met Ile Thr His Gly Cys Tyr Thr Arg Thr Arg His Lys His Lys Leu 
1               5                   10                  15      
Lys Lys Thr Leu Ile Met Leu Ser Ala Gly Leu Gly Leu Phe Phe Tyr 
            20                  25                  30          
Val Asn Gln Asn Ser Phe Ala Asn Gly Glu Asn Tyr Phe Lys Leu Gly 
        35                  40                  45              
Ser Asp Ser Lys Leu Leu Thr His Asp Ser Tyr Gln Asn Arg Leu Phe 
    50                  55                  60                  
Tyr Thr Leu Lys Thr Gly Glu Thr Val Ala Asp Leu Ser Lys Ser Gln 
65                  70                  75                  80  
Asp Ile Asn Leu Ser Thr Ile Trp Ser Leu Asn Lys His Leu Tyr Ser 
                85                  90                  95      
Ser Glu Ser Glu Met Met Lys Ala Ala Pro Gly Gln Gln Ile Ile Leu 
            100                 105                 110         
Pro Leu Lys Lys Leu Pro Phe Glu Tyr Ser Ala Leu Pro Leu Leu Gly 
        115                 120                 125             
Ser Ala Pro Leu Val Ala Ala Gly Gly Val Ala Gly His Thr Asn Lys 
    130                 135                 140                 
Leu Thr Lys Met Ser Pro Asp Val Thr Lys Ser Asn Met Thr Asp Asp 
145                 150                 155                 160 
Lys Ala Leu Asn Tyr Ala Ala Gln Gln Ala Ala Ser Leu Gly Ser Gln 
                165                 170                 175     
Leu Gln Ser Arg Ser Leu Asn Gly Asp Tyr Ala Lys Asp Thr Ala Leu 
            180                 185                 190         
Gly Ile Ala Gly Asn Gln Ala Ser Ser Gln Leu Gln Ala Trp Leu Gln 
        195                 200                 205             
His Tyr Gly Thr Ala Glu Val Asn Leu Gln Ser Gly Asn Asn Phe Asp 
    210                 215                 220                 
Gly Ser Ser Leu Asp Phe Leu Leu Pro Phe Tyr Asp Ser Glu Lys Met 
225                 230                 235                 240 
Leu Ala Phe Gly Gln Val Gly Ala Arg Tyr Ile Asp Ser Arg Phe Thr 
                245                 250                 255     
Ala Asn Leu Gly Ala Gly Gln Arg Phe Phe Leu Pro Ala Asn Met Leu 
            260                 265                 270         
Gly Tyr Asn Val Phe Ile Asp Gln Asp Phe Ser Gly Asp Asn Thr Arg 
        275                 280                 285             
Leu Gly Ile Gly Gly Glu Tyr Trp Arg Asp Tyr Phe Lys Ser Ser Val 
    290                 295                 300                 
Asn Gly Tyr Phe Arg Met Ser Gly Trp His Glu Ser Tyr Asn Lys Lys 
305                 310                 315                 320 
Asp Tyr Asp Glu Arg Pro Ala Asn Gly Phe Asp Ile Arg Phe Asn Gly 
                325                 330                 335     
Tyr Leu Pro Ser Tyr Pro Ala Leu Gly Ala Lys Leu Ile Tyr Glu Gln 
            340                 345                 350         
Tyr Tyr Gly Asp Asn Val Ala Leu Phe Asn Ser Asp Lys Leu Gln Ser 
        355                 360                 365             
Asn Pro Gly Ala Ala Thr Val Gly Val Asn Tyr Thr Pro Ile Pro Leu 
    370                 375                 380                 
Val Thr Met Gly Ile Asp Tyr Arg His Gly Thr Gly Asn Glu Asn Asp 
385                 390                 395                 400 
Leu Leu Tyr Ser Met Gln Phe Arg Tyr Gln Phe Asp Lys Ser Trp Ser 
                405                 410                 415     
Gln Gln Ile Glu Pro Gln Tyr Val Asn Glu Leu Arg Thr Leu Ser Gly 
            420                 425                 430         
Ser Arg Tyr Asp Leu Val Gln Arg Asn Asn Asn Ile Ile Leu Glu Tyr 
        435                 440                 445             
Lys Lys Gln Asp Ile Leu Ser Leu Asn Ile Pro His Asp Ile Asn Gly 
    450                 455                 460                 
Thr Glu His Ser Thr Gln Lys Ile Gln Leu Ile Val Lys Ser Lys Tyr 
465                 470                 475                 480 
Gly Leu Asp Arg Ile Val Trp Asp Asp Ser Ala Leu Arg Ser Gln Gly 
                485                 490                 495     
Gly Gln Ile Gln His Ser Gly Ser Gln Ser Ala Gln Asp Tyr Gln Ala 
            500                 505                 510         
Ile Leu Pro Ala Tyr Val Gln Gly Gly Ser Asn Ile Tyr Lys Val Thr 
        515                 520                 525             
Ala Arg Ala Tyr Asp Arg Asn Gly Asn Ser Ser Asn Asn Val Gln Leu 
    530                 535                 540                 
Thr Ile Thr Val Leu Ser Asn Gly Gln Val Val Asp Gln Val Gly Val 
545                 550                 555                 560 
Thr Asp Phe Thr Ala Asp Lys Thr Ser Ala Lys Ala Asp Asn Ala Asp 
                565                 570                 575     
Thr Ile Thr Tyr Thr Ala Thr Val Lys Lys Asn Gly Val Ala Gln Ala 
            580                 585                 590         
Asn Val Pro Val Ser Phe Asn Ile Val Ser Gly Thr Ala Thr Leu Gly 
        595                 600                 605             
Ala Asn Ser Ala Lys Thr Asp Ala Asn Gly Lys Ala Thr Val Thr Leu 
    610                 615                 620                 
Lys Ser Ser Thr Pro Gly Gln Val Val Val Ser Ala Lys Thr Ala Glu 
625                 630                 635                 640 
Met Thr Ser Ala Leu Asn Ala Ser Ala Val Ile Phe Phe Asp Gly Ala 
                645                 650                 655     
Pro Val Pro Tyr Pro Asp Pro Leu Glu Pro Ala Gln Pro Ala Met Ala 
            660                 665                 670         
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
        675                 680                 685             
Ser Leu Leu Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser Ser Tyr 
    690                 695                 700                 
Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
705                 710                 715                 720 
Ala Ala Ile Asn Trp Ser Gly Gly Ser Thr Ser Tyr Ala Asp Ser Val 
                725                 730                 735     
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Thr Lys Asn Thr Val Tyr 
            740                 745                 750         
Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Ala Phe Tyr Cys 
        755                 760                 765             
Ala Ala Thr Tyr Asn Pro Tyr Ser Arg Asp His Tyr Phe Pro Arg Met 
    770                 775                 780                 
Thr Thr Glu Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser 
785                 790                 795                 800 
Ser Glu Pro Lys Thr Pro Lys Pro Gln Pro Ala Ala Ala Glu Gln Lys 
                805                 810                 815     
Leu Ile Ser Glu Glu Asp Ala Ala Ala 
            820                 825 

<210> 26
<211> 5384
<212> DNA
<213> Artificial Sequence


<220> 
<223> >Regulatory circuit complete

<400> 26
ccggtctcga ggtgactgga aactggtgtt accttcgctg gcgcttgggc tgatatttgg       60

ttacgcaatg aaaaacagtg gcctgtggct ggcggcgcgt agtgcaaaga cggcgcaccg      120

tgagcaggaa atcaaaaata aagcgtgagg ggcactcacg ctttcgctta aacagtaaaa      180

tgccggatga taattccggc ttttttatct gtcaggattc cggtggaacc gacatatggc      240

ggtatttcac cagaatgtca ttctgccgtt ctgctttatt ttgcaaatcc cacagaccac      300

ggtcgatacc atcattaatc aggaaaatga cccctgtttc gatagccgac atcaggcaca      360

gcataacagg ttcgttcgag gtgtaaccca cttccccttc aagcaagcgc tggtagtcaa      420

taaagcggaa aaccccggcc tgaacttcat aggaaagtat cgtcttactg gtgttcaccg      480

aagaaaggat ctcgccggta ctcacattga cgacgcgcag gttcacggca atctgagagc      540

tccaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt      600

cggtgaacgc tctcctgagt aggacaaatg gatcctcagc caaacgtctc ttcaggccac      660

tgactagcga taactttccc cacaacggaa caactctcat tgcatgggat cattgggtac      720

tgtgggttta gtggttgtaa aaacacctga ccgctatccc tgatcagttt cttgaaggta      780

aactcatcac ccccaagtct ggctatgcag aaatcacctg gctcaacagc ctgctcaggg      840

tcaacgagaa ttaacattcc gtcaggaaag cttggcttgg agcctgttgg tgcggtcatg      900

gaattacctt caacctcaag ccagaatgca gaatcactgg cttttttggt tgtgcttacc      960

catctctccg catcaccttt ggtaaaggtt ctaagcttag gtgagaacat ccctgcctga     1020

acatgagaaa aaacagggta ctcatactca cttctaagtg acggctgcat actaaccgct     1080

tcatacatct cgtagatttc tctggcgatt gaagggctaa attcttcaac gctaactttg     1140

agaatttttg caagcaatgc ggcgttataa gcatttaatg cattgatgcc attaaataaa     1200

gcaccaacgc ctgactgccc catccccatc ttgtctgcga cagattcctg ggataagcca     1260

agttcatttt tctttttttc ataaattgct ttaaggcgac gtgcgtcctc aagctgctct     1320

tgtgttaatg gtttcttttt tgtgctcatc tagtatttct cctctttaat ctgcagttct     1380

ctatcactga tagggagtgg taaaataact ctatcaatga tagagtgtca acaaaaatta     1440

ggaattaatg atgtccagat tagataaaag taaagtgatt aacagcgcat tagagctgct     1500

taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga     1560

gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat     1620

tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga     1680

ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc     1740

aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt     1800

agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt     1860

ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga     1920

aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt     1980

tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatta tatgcggatt     2040

agaaaaacaa cttaaatgtg aaagtgggtc ttaagtcgac cttggactcc tgttgataga     2100

tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc     2160

gttttttatt ggtgagaatc cagccgcggt aacaccgtgc gtgttgacta ttttacctct     2220

ggcggtgata atggttgcga attcattaaa gaggagaaat actagatgaa accagtaacg     2280

ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac     2340

caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg     2400

aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacaaagctt gctgattggc     2460

gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct     2520

cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa     2580

gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac     2640

tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg     2700

ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac     2760

ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta     2820

gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc     2880

actcgcaatc aaattcagcc gatagcggaa cgggaaggcg acttcagtgc catgtccggt     2940

tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc     3000

aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt     3060

gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg     3120

ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg     3180

caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa     3240

agaaaaacca cgctagcacc caatacgcaa accgcctctc cccgcgcgtt ggccgattca     3300

ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcaggcagc aaacgacgaa     3360

aactacgctg catcagtttg aggtaccgcg caacgcaatt aatgtgagtt agctcactca     3420

ttaggcaccc caggcactag tctgcgcgaa ggcagttcag ggcaaagtca gacgaagcaa     3480

gaaaagaccc tttcattgcc tgctaatcaa cccattgctt tgacgaagtt gagtttaaat     3540

atttccccgg acgatcgggt gaaaatagtt gttactgttt ctgatggaca gtcacttcat     3600

ttatcacaac aatggccgcc ctcttcagaa aagtcttaat ttgttgaaat atcgagcata     3660

agatgaatct ggagagaatg gtctgctgcg aatcagccaa cctgaaagta tggataacac     3720

aaccctcaag gatgactaat cattgaggaa atagaataaa tgttcagacc ttttttaaac     3780

tctcttatgc tcggcagttt gttttttcct tttattgcca ttgctggaag caccgtgcaa     3840

gggggcgtga tccattttta tggccaaatt gtggaaccgg catgtgacgt cagcacccag     3900

tcatcacccg tagaaatgaa ctgcccacag aatggttcta ttccgggcaa aacctactcc     3960

agcaaagcgt taatgagcgg caatgtcaaa aacgcgcaaa tagcctcagg catgcaccgg     4020

gattaccctg ttatccctat tattagaaaa attcatccag catcagatga aattgcagtt     4080

tgttcatatc cggattatca atgccatatt tctgaaacag acgtttttgc aggctcgggc     4140

taaattcgcc caggcagttc cacagaatgg ccagatcctg ataacgatcc gcaatgccca     4200

cacggcccac atcaatgcag ccaatcagtt tgccttcatc gaaaatcagg ttatccaggc     4260

taaaatcgcc gtgggtcacc acgctatccg ggctaaacgg cagcagttta tgcatttctt     4320

tccacacctg ttccaccggc cagccgttac gttcatcatc aaaatcgctc gcatccacca     4380

ggccgttgtt catacggctc tgcgcctggg ccagacgaaa cacacgatcg ctgttaaacg     4440

ggcagttgca caccggaatg ctatgcagac gacgcagaaa cacggccagc gcatccacaa     4500

tgttttcgcc gctatccgga tattcttcca gcacctgaaa cgcggttttg cccggaatcg     4560

cggtggtcag cagccacgca tcatccgggg tgcgaataaa atgtttaatg gtcggcagcg     4620

gcataaattc ggtcagccag ttcagacgca ccatttcatc ggtcacatcg ttcgccacgc     4680

tgcctttgcc atgtttcaga aacagttccg gcgcatccgg tttgccatac agacgataaa     4740

tggtcgcgcc gctctgaccc acgttatcac gcgcccattt atagccatac agatccgcat     4800

ccatgttgct gttcagacgc ggacggctac agctcgtttc acgctgaata tggctcataa     4860

caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt     4920

tatcttgtgc aatgtaacat cagagatttt gagacacaag acgtcggcgc gccgatctga     4980

agatcagcag ttcaacctgt tgatagtacg tactaagctc tcatgtttca cgtactaagc     5040

tctcatgttt aacgtactaa gctctcatgt ttaacgaact aaaccctcat ggctaacgta     5100

ctaagctctc atggctaacg tactaagctc tcatgtttca cgtactaagc tctcatgttt     5160

gaacaataaa attaatataa atcagcaact taaatagcct ctaaggtttt aagttttata     5220

agaaaaaaaa gaatatataa ggcttttaaa gcctttaagg tttaacggtt gtggacaaca     5280

agccagggat gtaacgcact gagaagccct tagagcctct caaagcaatt ttgagtgaca     5340

caggaacact taacggctga catggattac cctgttatcc ctac                      5384


<210> 27
<211> 57
<212> DNA
<213> Artificial Sequence


<220> 
<223> >F_XbaI_RBS_MAP

<400> 27
tcgagtctag aaagaaggag atatacatat gtttagtcca acggcaatgg taggtag        57


<210> 28
<211> 37
<212> DNA
<213> Artificial Sequence


<220> 
<223> R_NotI_MAP

<400> 28
ccgatgcggc cgccagccga gtatcctgca cattgtc                              37


<210> 29
<211> 27
<212> DNA
<213> Artificial Sequence


<220> 
<223> >pjA_5'

<400> 29
agctgtgcga acgtggtatt aactttc                                        27


<210> 30
<211> 27
<212> DNA
<213> Artificial Sequence


<220> 
<223> ypjA_3'

<400> 30
aacaacacta tggcctgaca ctgaacg                                        27


<210> 31
<211> 25
<212> DNA
<213> Artificial Sequence


<220> 
<223> >R_AraC_test

<400> 31
cgatcaactc tatttctcgc gggta                                          25


<210> 32
<211> 27
<212> DNA
<213> Artificial Sequence


<220> 
<223> F_CesT_test

<400> 32
gtgggccata cctgtgttat gagtcag                                        27


<210> 33
<211> 47
<212> DNA
<213> Artificial Sequence


<220> 
<223> F_SacI_RBS_CesT

<400> 33
aattcgagct caagaaggag atatacatat gtcatcaaga tccgaac                   47


<210> 34
<211> 36
<212> DNA
<213> Artificial Sequence


<220> 
<223> F_SfiI_Gly_ZF6x

<400> 34
attgcggccc agccggccgg aaccggggcc gagcgc                               36


<210> 35
<211> 34
<212> DNA
<213> Artificial Sequence


<220> 
<223> >R_NotI_ZF6x

<400> 35
ccgatgcggc cgcgactagt tgtgagccgc cgcc                                 34


<210> 36
<211> 41
<212> DNA
<213> Artificial Sequence


<220> 
<223> F_SfiI_GranzymeB

<400> 36
attgcggccc agccggccat gattattggc ggccatgaag c                         41


<210> 37
<211> 43
<212> DNA
<213> Artificial Sequence


<220> 
<223> R_NotI_GranzymeB

<400> 37
ccgatgcggc cgcatagcgt ttcatggttt ttttaatcca atg                       43


<210> 38
<211> 36
<212> DNA
<213> Artificial Sequence


<220> 
<223> >F_SfiI_OVA

<400> 38
attgcggccc agccggccat gggcagcatt ggcgcg                               36


<210> 39
<211> 29
<212> DNA
<213> Artificial Sequence


<220> 
<223> R_NotI_OVA

<400> 39
ccgatgcggc cgccgggctc acgcagcgg                                      29


<210> 40
<211> 40
<212> DNA
<213> Artificial Sequence


<220> 
<223> F_SfiI_TK

<400> 40
attgcggccc agccggccat ggcttcgtac ccctgccatc                           40


<210> 41
<211> 34
<212> DNA
<213> Artificial Sequence


<220> 
<223> R_NotI_TK

<400> 41
ccgatgcggc cgcgttagcc tcccccatct cccg                                 34


<210> 42
<211> 63
<212> DNA
<213> Artificial Sequence


<220> 
<223> >F_SacI_RBS_EspF20

<400> 42
aattcgagct caagcttaag aaggagatat acatatgctt aatggaatta gtaacgctgc     60

ttc                                                                   63


<210> 43
<211> 38
<212> DNA
<213> Artificial Sequence


<220> 
<223> >R_SpeI_Bla

<400> 43
catgcactag tttaccaatg cttaatcagt gaggcacc                             38


<210> 44
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> R_NotI_BIM

<400> 44
ccgatgcggc cgcatgcatg cgccacacca gg                                   32


<210> 45
<211> 37
<212> DNA
<213> Artificial Sequence


<220> 
<223> F_SfiI_BIM

<400> 45
ggtgcggccc agccggccgc gaaacagccg agcgatg                              37


<210> 46
<211> 53
<212> DNA
<213> Artificial Sequence


<220> 
<223> >F_SfiI_Cas9

<400> 46
ggtgcggccc agccggccat ggataagaaa tactcaatag gcttagatat cgg            53


<210> 47
<211> 41
<212> DNA
<213> Artificial Sequence


<220> 
<223> R_NotI_Cas9

<400> 47
ccgatgcggc cgcgtcacct cctagctgac tcaaatcaat g                         41


<210> 48
<211> 41
<212> DNA
<213> Artificial Sequence


<220> 
<223> >F_SfiI_Sox2

<400> 48
ggtgcggccc agccggccat gatggagacg gagctgaagc c                         41


<210> 49
<211> 33
<212> DNA
<213> Artificial Sequence


<220> 
<223> >R_NotI_Sox2

<400> 49
ccgatgcggc cgccatgtgc gacaggggca gtg                                  33


<210> 50
<211> 40
<212> DNA
<213> Artificial Sequence


<220> 
<223> >F_SfiI_Oct4

<400> 50
ggtgcggccc agccggccat ggctggacac ctggcttcag                           40


<210> 51
<211> 35
<212> DNA
<213> Artificial Sequence


<220> 
<223> R_NotI_Oct4

<400> 51
ccgatgcggc cgcgtttgaa tgcatgggag agccc                                35


<210> 52
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> >R_cargo_seq

<400> 52
gcgtcgaagc atggtgatgg t                                              21


<210> 53
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> 3' del LacI confirm

<400> 53
attgatggtg aacatgatgc cgac                                           24


<210> 54
<211> 39
<212> DNA
<213> Artificial Sequence


<220> 
<223> R_NotI_tBID

<400> 54
ccgatgcggc cgcatccata ccattacgtg ccaggctac                            39


<210> 55
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> >5prima_SReginteg

<400> 55
cgtttggctg aggatccatt tgtc                                           24


<210> 56
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> F_LacI_seq

<400> 56
gcgtctgcgt ctggctggc                                                 19


<210> 57
<211> 31
<212> DNA
<213> Artificial Sequence


<220> 
<223> R_3prima de LacI

<400> 57
ctaatgagtg agctaactca cattaattgc g                                   31


<210> 58
<211> 58
<212> DNA
<213> Artificial Sequence


<220> 
<223> >F_EcoRI_RBS0034_LacI

<400> 58
gttgcgaatt caaagaggag aaatactaga tgaaaccagt aacgttatac gatgtcgc       58


<210> 59
<211> 66
<212> DNA
<213> Artificial Sequence


<220> 
<223> >R_KpnI_ssrAtag(LAA)_LacI

<400> 59
tgcgcggtac ctcaagcagc cagagcgtag ttttcgtcgt tagcagcctg cccgctttcc     60

agtcgg                                                                66


<210> 60
<211> 44
<212> DNA
<213> Artificial Sequence


<220> 
<223> F_EcoRI_RBS0034_LacI (NEW)

<400> 60
gttgcgaatt caaagaggag aaatactaga tgaaaccagt aacg                      44


<210> 61
<211> 44
<212> DNA
<213> Artificial Sequence


<220> 
<223> >F_EcoRI_RBS0034_(GTG)LacI

<400> 61
gttgcgaatt caaagaggag aaatactagg tgaaaccagt aacg                      44


<210> 62
<211> 61
<212> DNA
<213> Artificial Sequence


<220> 
<223> >F_EcoRI_RBSWT_(GTG)LacI

<400> 62
gttgcgaatt cagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg     60

c                                                                     61


<210> 63
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> R_KpnI_LacI

<400> 63
tgcgcggtac ctcactgccc gctttccagt cg                                   32


<210> 64
<211> 66
<212> DNA
<213> Artificial Sequence


<220> 
<223> R_KpnI_ssrAtag(ASV)_LacI

<400> 64
tgcgcggtac ctcaaactga tgcagcgtag ttttcgtcgt ttgctgcctg cccgctttcc     60

agtcgg                                                                66


<210> 65
<211> 66
<212> DNA
<213> Artificial Sequence


<220> 
<223> R_KpnI_ssrAtag(AAV)_LacI

<400> 65
tgcgcggtac ctcaaactgc tgcagcgtag ttttcgtcgt ttgctgcctg cccgctttcc     60

agtcgg                                                                66


<210> 66
<211> 33
<212> DNA
<213> Artificial Sequence


<220> 
<223> F_SfiI_PE25

<400> 66
ggtgcggccc agccggccgg cccggcggac agc                                  33


<210> 67
<211> 31
<212> DNA
<213> Artificial Sequence


<220> 
<223> R_NotI_PE

<400> 67
ccgatgcggc cgccttcagg tcctcgcgcg g                                   31


<210> 68
<211> 36
<212> DNA
<213> Artificial Sequence


<220> 
<223> R_BglII_STOP_Tir

<400> 68
ttcttagatc tttaaacgaa acgtactggt cccggc                               36


<210> 69
<211> 34
<212> DNA
<213> Artificial Sequence


<220> 
<223> R_BglII_STOP_PE25

<400> 69
ttcttagatc ttcacttcag gtcctcgcgc ggcg                                 34


<210> 70
<211> 42
<212> DNA
<213> Artificial Sequence


<220> 
<223> F_SfiI_TccC3

<400> 70
ggtgcggccc agccggccat gccgaccatt gcagaacgta tt                        42


<210> 71
<211> 40
<212> DNA
<213> Artificial Sequence


<220> 
<223> R_BglII_STOP_TccC3

<400> 71
ttcttagatc ttcaacgttt atgcggtttc acatttttca                           40


