               SEQUENCE LISTING

<110> Global Bioenergies

<120> Improved means and methods for producing isobutene from 3-methylcrotonic acid

<130> AD3993 PCT S3

<150> EP 20 215 872.1
<151> 2020-12-21

<160> 1

<170> BiSSAP 1.3.6

<210> 1
<211> 10770
<212> DNA
<213> Artificial Sequence


<220> 
<223> recombinant pGB 5550 plasmid

<400> 1
ctcactactt tagtcagttc cgcagtatta caaaaggatg tcgcaaacgc tgtttgctcc       60

tctacaaaac agaccttaaa accctaaagg cttaagtagc accctcgcaa gctcgggcaa      120

atcgctgaat attccttttg tctccgacca tcaggcacct gagtcgctgt ctttttcgtg      180

acattcagtt cgctgcgctc acggctctgg cagtgaatgg gggtaaatgg cactacaggc      240

gccttttatg gattcatgca aggaaactac ccataataca agaaaagccc gtcacgcttc      300

tcagggcgtt ttatggcggg tctgctatgt ggtgctatct gactttttgc tgttcagcag      360

ttcctgccct ctgattttcc agtctgaccc tagtcaaggc cttaagtgag tcgtattacg      420

gactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc      480

gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc      540

gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc      600

ttacgcatct gtgcggtatt tcacaccgcc cggggaacta tagtttaaac ttttcaatga      660

attcatttaa gcggccgcat caattctaga atttaaatag tcaaaagcct ccgaccggag      720

gcttttgact gacctattga caattaaagg ctaaaatgct ataattccac taatagaaat      780

aattttgttt aactttaggt ctctatcgta agaaggagat atacatatga aagaagtggt      840

gattgccagc gcagttcgta ccgcaattgg tagctatggt aaaagcctga aagatgttcc      900

ggcagttgat ctgggtgcaa ccgcaattaa agaagcagtt aaaaaagccg gtattaaacc      960

ggaagatgtg aacgaagtta ttctgggtaa tgttctgcaa gcaggtctgg gtcagaatcc     1020

ggcacgtcag gcctcgttta aagcaggtct gccggttgaa attccggcaa tgaccattaa     1080

caaagtttgt ggtagcggtc tgcgtaccgt tagcctggca gcacagatta tcaaagccgg     1140

tgatgcagat gttattattg ccggtggtat ggaaaatatg agccgtgcac cgtatctggc     1200

aaataatgca cgttggggtt atcgtatggg taatgccaaa tttgtggatg agatgattac     1260

cgatggtctg tgggatgcct ttaatgatta tcacatgggt attaccgcag agaatattgc     1320

agaacgttgg aatattagcc gtgaagaaca ggatgaattt gcactggcaa gccagaaaaa     1380

agcagaagaa gcaattaaaa gcggtcagtt caaagatgaa attgtgccgg ttgttatcaa     1440

aggtcgtaaa ggtgaaaccg ttgttgatac cgatgaacat ccgcgttttg gtagcaccat     1500

tgaaggtctg gcaaaactga aaccggcatt caaaaaagat ggcaccgtta ccgcaggtaa     1560

tgcaagcggt ctgaatgatt gtgcagcagt tctggttatt atgagcgcag aaaaagcaaa     1620

agaactgggt gttaaaccgc tggcaaaaat tgtgagctat ggtagtgccg gtgttgatcc     1680

ggcaattatg ggttatggtc cgttttatgc aaccaaagca gcaattgaaa aagcaggttg     1740

gaccgttgat gaactggatc tgattgaaag caatgaagca tttgcagcac agagcctggc     1800

agttgcaaaa gacctgaaat tcgatatgaa taaagtgaat gtgaatggcg gtgcaattgc     1860

cctgggtcat ccgattggtg caagcggtgc acgtattctg gttaccctgg ttcatgcaat     1920

gcagaaacgt gatgcaaaaa aaggtctggc caccctgtgt attggtggtg gtcagggcac     1980

cgcaattctg ctggaaaaat gctaataagc ttgaaggaga tataatgacc attggtattg     2040

ataaaatcag ctttttcgtg cctccgtact atattgatat gaccgcactg gccgaagcac     2100

gtaatgttga tccgggtaaa tttcatattg gtattggtca ggatcagatg gccgttaatc     2160

cgattagcca ggatattgtt acctttgcag caaatgcagc agaagcaatt ctgaccaaag     2220

aagataaaga ggccattgat atggttattg ttggcaccga aagcagcatt gatgaaagca     2280

aagcagcagc agttgttctg catcgtctga tgggtattca gccgtttgca cgtagctttg     2340

aaattaaaga agcatgttac ggagcaaccg caggtctgca actggcaaaa aatcatgttg     2400

cactgcatcc ggataaaaaa gttctggttg ttgcagcaga tattgccaaa tatggtctga     2460

atagcggtgg tgaaccgacc cagggtgccg gtgcagttgc aatgctggtt gcaagcgaac     2520

cgcgtattct ggcactgaaa gaagataatg ttatgctgac ccaggatatt tatgattttt     2580

ggcgtccgac cggtcatccg tatccgatgg ttgatggtcc gctgagcaat gaaacctata     2640

ttcagagctt tgcacaggtg tgggatgaac ataaaaaacg taccggtctg gatttcgcag     2700

attatgatgc actggcattt catatcccgt ataccaaaat gggtaaaaaa gcactgctgg     2760

ccaaaattag cgatcagacc gaagccgaac aagaacgcat tctggcacgt tatgaagaaa     2820

gcattgttta tagccgtcgt gtgggtaatc tgtataccgg tagcctgtat ctgggtctga     2880

ttagcctgct ggaaaatgca accaccctga ccgcaggtaa tcagattggt ctgtttagct     2940

atggtagcgg tgccgttgca gaatttttca caggtgaact ggttgcaggt tatcagaatc     3000

atctgcaaaa agaaacccat ctggcactgc tggataatcg taccgaactg agcattgcag     3060

aatatgaagc aatgtttgca gaaaccctgg ataccgatat tgatcagacc ctggaagatg     3120

aactgaaata tagcattagc gccattaata acaccgtgcg tagctatcgt aactaataag     3180

gtagaaggag atataatgcc ggaatttaaa gttgatgcac gtggtccgat tgaaatctgg     3240

accattgatg gtgaaagccg tcgtaatgca attagccgtg caatgctgaa agaactgggt     3300

gaactggtta cccgtgttag cagcagccgt gatgttcgtg cagttgttat taccggtgcc     3360

ggtgataaag cattttgtgc cggtgccgat ctgaaagaac gtgcaacaat ggccgaagat     3420

gaagttcgtg catttctgga tggtctgcgt cgtacctttc gtgcaattga aaaaagcgat     3480

tgcgttttta ttgcagccat taatggtgcc gcactgggtg gtggcaccga actggcactg     3540

gcatgtgatc tgcgtgttgc agcaccggca gcagaactgg gtctgaccga agttaaactg     3600

ggcattattc cgggtggtgg tggtacacag cgtctggcac gtctggttgg tccgggtcgt     3660

gcaaaagacc tgattctgac cgcacgtcgt attaatgcag cagaagcatt tagcgttggt     3720

ctggcaaatc gtctggcacc ggaaggtcat ctgctggcag ttgcctatgg tctggcagaa     3780

agcgttgttg aaaatgcacc gattgcagtt gcaaccgcaa aacatgcaat tgatgaaggc     3840

accggtctgg aactggatga tgcactggca ctggaactgc gtaaatatga agaaattctg     3900

aaaaccgaag atcgcctgga aggtctgcgt gcatttgcag aaaaacgtgc accggtttat     3960

aaaggtcgct aataagaacg aaggagatat aatgaaaacc gcacgttggt gtagcctgga     4020

agaagcagtt gcaagcattc cggatggtgc aagcctggca accggtggtt ttatgctggg     4080

tcgtgcaccg atggcactgg ttatggaact gattgcacag ggtaaacgtg atctgggtct     4140

gattagcctg ccgaatccgc tgccagcaga atttctggtt gccggtggtt gtctggctcg     4200

tctggaaatt gcatttggtg cactgagtct gcaaggtcgt gttcgtccga tgccgtgtct     4260

gaaacgtgca atggaacagg gcaccctggc atggcgtgaa catgatggtt atcgtgttgt     4320

tcagcgtctg cgtgcagcaa gcatgggtct gccgtttatt ccggcaccgg atgcagatgt     4380

tagcggtctg gcacgtaccg aaccgcctcc gaccgttgaa gatccgttta ccggtctgcg     4440

tgttgcagtt gaaccggcat tttatccgga tgttgcactg ctgcacgcac gtgcagccga     4500

tgaacgtggt aatctgtata tggaagatcc gaccaccgat ctgctggttg cgggtgcagc     4560

aaaacgtgtt attgcaaccg ttgaagaacg tgttgcaaaa ctgcctcgtg caaccctgcc     4620

tggttttcag gttgatcgta ttgttctggc accgggtggt gcactgccga ccggttgtgc     4680

aggtctgtat ccgcatgatg atgaaatgct ggcacgttat ctgagcctgg cagaaaccgg     4740

tcgtgaagcc gaatttctgg aaaccctgct gacccgtcgt gcagcataat gaggatccga     4800

aggagatata catatgagcg caaccctgga tattacaccg gcagaaaccg ttgttagcct     4860

gctggcacgt cagattgatg atggtggtgt tgttgcaacc ggtgttgcaa gtccgctggc     4920

aattctggcc attgcagttg cacgtgccac ccatgcaccg gatctgacct atctggcatg     4980

tgttggtagc ctggacccgg aaattccgac cctgctgccg agcagcgaag acctgggtta     5040

tctggatggt cgtagcgcag aaattaccat tccggacctg tttgatcatg cacgtcgtgg     5100

tcgtgttgat accgtttttt ttggtgcagc cgaagttgat gccgaaggtc gtaccaatat     5160

gaccgcaagc ggtagtctgg ataaaccgcg taccaaattt ccgggtgttg ccggtgcagc     5220

caccctgcgt cagtgggttc gtcgtccggt tctgctggtt ccgcgtcaga gccgtcgtaa     5280

tctggttccg gaagttcagg ttgcaaccac ccgtgatccg cgtcgtccgg tgaccctgat     5340

tagcgatctg ggtgtttttg aactgggtgc aagcggtgca cgtctgctgg cacgccatcc     5400

gtgggcaagc gaagaacata ttgcagaacg taccggtttt gcatttcagg ttagcgaagc     5460

actgagcgtt accagcctgc cggatgcacg taccgttgca gcaattcgtg caattgatcc     5520

gcatggctat cgtgatgcac tggttggtgc ataataataa gagagaccag cctgatacag     5580

attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg     5640

gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt     5700

gtggggtcac cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca     5760

gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaactact agaatttaaa     5820

tagtcaaaag cctccgaccg gaggcttttg actgacactt tgacacaccc tttacccccc     5880

ttataattaa cgtaatagaa ataattttgt ttaactttag gtctctatcg accataatta     5940

attaacttta agaaggagat atacatatga gtcaggcgct aaaaaattta ctgacattgt     6000

taaatctgga aaaaattgag gaaggactct ttcgcggcca gagtgaagat ttaggtttac     6060

gccaggtgtt tggcggccag gtcgtgggtc aggccttgta tgctgcaaaa gagacggtcc     6120

ctgaagaacg gctggtacat tcgtttcaca gctactttct tcgccctggc gatagtaaga     6180

agccgattat ttatgatgtc gaaacgctgc gtgacggtaa cagcttcagc gcccgccggg     6240

ttgctgctat tcaaaacggc aaaccgattt tttatatgac tgcctctttc caggcaccag     6300

aagcgggttt cgaacatcaa aaaacaatgc cgtccgcgcc agcgcctgat ggcctccctt     6360

cggaaacgca aatcgcccaa tcgctggcgc acctgctgcc gccagtgctg aaagataaat     6420

tcatctgcga tcgtccgctg gaagtccgtc cggtggagtt tcataaccca ctgaaaggtc     6480

acgtcgcaga accacatcgt caggtgtgga ttcgcgcaaa tggtagcgtg ccggatgacc     6540

tgcgcgttca tcagtatctg ctcggttacg cttctgatct taacttcctg ccggtagctc     6600

tacagccgca cggcatcggt tttctcgaac cggggattca gattgccacc attgaccatt     6660

ccatgtggtt ccatcgcccg tttaatttga atgaatggct gctgtatagc gtggagagca     6720

cctcggcgtc cagcgcacgt ggctttgtgc gcggtgagtt ttatacccaa gacggcgtac     6780

tggttgcctc gaccgttcag gaaggggtga tgcgtaatca caattaataa ccatggttat     6840

aagagagacc agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa     6900

tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa     6960

acgccgtagc gccgatggta gtgtggggtc accccatgcg agagtaggga actgccaggc     7020

atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt     7080

cggtgaacta ctagttggcg ggcggccgct tagctctgca gatgagaaat tcttgaagac     7140

gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttaag     7200

cttcttagaa tagctcttct atgaggtggc acttttcggg gaaagatatc cgcatatatg     7260

gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta     7320

tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc     7380

tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc     7440

tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc     7500

tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatcggt acctttcatg     7560

atatatctcc caatttgtgt agggcttatt atgcacgctt aaaaataata aaagcagact     7620

tgacctgata gtttggctgt gagcaattat gtgcttagtg catctaacgc ttgagttaag     7680

ccgcgccgcg aagcggcgtc ggcttgaacg aattgttaga cattatttgc cgactacctt     7740

ggtgatctcg cctttcacgt agtggacaaa ttcttccaac tgatctgcgc gcgaggccaa     7800

gcgatcttct tcttgtccaa gataagcctg tctagcttca agtatgacgg gctgatactg     7860

ggccggcagg cgctccattg cccagtcggc agcgacatcc ttcggcgcga ttttgccggt     7920

tactgcgctg taccaaatgc gggacaacgt aagcactaca tttcgctcat cgccagccca     7980

gtcgggcggc gagttccata gcgttaaggt ttcatttagc gcctcaaata gatcctgttc     8040

aggaaccgga tcaaagagtt cctccgccgc tggacctacc aaggcaacgc tatgttctct     8100

tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg gctggctcga agatacctgc     8160

aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg gataacgcca     8220

cggaatgatg tcgtcgtgca caacaatggt gacttctaca gcgcggagaa tctcgctctc     8280

tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc     8340

aagccttacg gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca ggccgccatc     8400

cactgcggag ccgtacaaat gtacggccag caacgtcggt tcgagatggc gctcgatgac     8460

gccaactacc tctgatagtt gagtcgatac ttcggcgatc accgcttccc tcatgatgtt     8520

taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa     8580

acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc     8640

aaaaaaacag tcataacaag ccatgaaaac cgccacgagc tcctgtcaga ccaagtttac     8700

gagctcgctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc atctggattt     8760

gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatccaa gcactaggga     8820

cagtaagacg ggtaagcctg ttgatgatac cgctgcctta ctgggtgcat tagccagtct     8880

gaatgacctg tcacgggata atccgaagtg gtcagactgg aaaatcagag ggcaggaact     8940

gctgaacagc aaaaagtcag atagcaccac atagcagacc cgccataaaa cgccctgaga     9000

agcccgtgac gggcttttct tgtattatgg gtagtttcct tgcatgaatc cataaaaggc     9060

gcctgtagtg ccatttaccc ccattcactg ccagagccgt gagcgcagcg aactgaatgt     9120

cacgaaaaag acagcgactc aggtgcctga tggtcggaga caaaaggaat attcagcgat     9180

ttgcccgagc ttgcgagggt gctacttaag cctttagggt tttaaggtct gttttgtaga     9240

ggagcaaaca gcgtttgcga catccttttg taatactgcg gaactgacta aagtagtgag     9300

ttatacacag ggctgggatc tattcttttt atcttttttt attctttctt tattctataa     9360

attataacca cttgaatata aacaaaaaaa acacacaaag gtctagcgga atttacagag     9420

ggtctagcag aatttacaag ttttccagca aaggtctagc agaatttaca gatacccaca     9480

actcaaagga aaaggacatg taattatcat tgactagccc atctcaattg gtatagtgat     9540

taaaatcacc tagaccaatt gagatgtatg tctgaattag ttgttttcaa agcaaatgaa     9600

ctagcgatta gtcgctatga cttaacggag catgaaacca agctaatttt atgctgtgtg     9660

gcactactca accccacgat tgaaaaccct acaaggaaag aacggacggt atcgttcact     9720

tataaccaat acgctcagat gatgaacatc agtagggaaa atgcttatgg tgtattagct     9780

aaagcaacca gagagctgat gacgagaact gtggaaatca ggaatccttt ggttaaaggc     9840

tttgagattt tccagtggac aaactatgcc aagttctcaa gcgaaaaatt agaattagtt     9900

tttagtgaag agatattgcc ttatcttttc cagttaaaaa aattcataaa atataatctg     9960

gaacatgtta agtcttttga aaacaaatac tctatgagga tttatgagtg gttattaaaa    10020

gaactaacac aaaagaaaac tcacaaggca aatatagaga ttagccttga tgaatttaag    10080

ttcatgttaa tgcttgaaaa taactaccat gagtttaaaa ggcttaacca atgggttttg    10140

aaaccaataa gtaaagattt aaacacttac agcaatatga aattggtggt tgataagcga    10200

ggccgcccga ctgatacgtt gattttccaa gttgaactag atagacaaat ggatctcgta    10260

accgaacttg agaacaacca gataaaaatg aatggtgaca aaataccaac aaccattaca    10320

tcagattcct acctacgtaa cggactaaga aaaacactac acgatgcttt aactgcaaaa    10380

attcagctca ccagttttga ggcaaaattt ttgagtgaca tgcaaagtaa gcatgatctc    10440

aatggttcgt tctcatggct cacgcaaaaa caacgaacca cactagagaa catactggct    10500

aaatacggaa ggatctgagg ttcttatggc tcttgtatct atcagtgaag catcaagact    10560

aacaaacaaa agtagaacaa ctgttcaccg ttagatatca aagggaaaac tgtccataag    10620

cacagatgaa aacggtgtaa aaaagataga tacatcagag cttttacgag tttttggtgc    10680

atttaaagct gttcaccatg aacagatcga caatgtaacg catgcaccga gcgcagcgag    10740

tcagtgagcg aggaagcgga acagcgcctg                                     10770


