                         SEQUENCE LISTING

<110>  The University of Melbourne
 
<120>  Coronavirus vaccine

<130>  531558782AZD

<150>  AU 2020904820
<151>  2020-12-23

<150>  AU 2021903911
<151>  2021-12-02

<160>  33    

<170>  PatentIn version 3.5

<210>  1
<211>  1383
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD-mouse IgG1 Fc-fusion nucleotide sequence

<400>  1
gaattcaagc ttgccaccat ggggattctt cccagccctg ggatgcctgc gctgctctcc       60

ctcgtgagcc ttctctccgt gctgctgatg ggttgcgtag ctgaaaccgg taacttgtgc      120

ccttttggtg aagtttttaa cgccaccaga tttgcatctg tttatgcttg gaacaggaag      180

agaatcagca actgtgttgc tgattattct gtcctatata attccgcatc attttccact      240

tttaagtgtt atggagtgtc tcctactaaa ttaaatgatc tctgctttac taatgtctat      300

gcagattcat ttgtaattag aggtgatgaa gtcagacaaa tcgctccagg gcaaactgga      360

aagattgctg attataatta taaattacca gatgatttta caggctgcgt tatagcttgg      420

aactctaaca atcttgattc taaggttggt ggtaattata attacctgta tagattgttt      480

aggaagtcta atctcaaacc ttttgagaga gatatttcaa ctgaaatcta tcaggccggt      540

agcacacctt gtaatggtgt tgaaggtttt aattgttact ttcctttaca atcatatggt      600

ttccaaccca ctaatggtgt tggttaccaa ccatacagag tagtagtact ttcttttgaa      660

cttctacatg caccagcaac tgtttgtgga cctggatccg ggagcggctg caaaccctgt      720

atctgcaccg tgcctgaagt ctcctccgtc tttatcttcc cccccaagcc taaggacgtc      780

ctgaccatca ccctcacccc taaggtcacc tgcgtcgtgg tcgatatatc caaagatgac      840

cccgaggtgc agttcagctg gttcgtggac gatgtggaag tccacaccgc ccagacccaa      900

cccagggagg agcagttcaa ctccaccttc aggtccgtga gcgagctgcc cattatgcac      960

caggactggc tcaacggaaa ggaatttaag tgtagagtca acagcgctgc cttccccgcc     1020

cccatcgaaa agacaatctc caagaccaag ggcaggccta aagctcctca ggtctacacc     1080

atcccccccc ccaaggagca gatggccaag gacaaggtga gcctcacctg catgatcaca     1140

gacttcttcc ccgaggatat aaccgtggag tggcagtgga atggccagcc cgccgagaac     1200

tataagaaca cccagcccat tatggacacc gacggcagct atttcgtcta tagcaagctg     1260

aacgtccaga agtccaattg ggaagccggc aacaccttta cctgctccgt gctgcatgag     1320

ggcctgcaca atcaccacac cgagaagagc ctgagccact cccccggcaa gtgatgactc     1380

gag                                                                   1383


<210>  2
<211>  457
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD-mouse IgG1 Fc-fusion amino acid sequence

<400>  2

Glu Phe Lys Leu Ala Thr Met Gly Ile Leu Pro Ser Pro Gly Met Pro 
1               5                   10                  15      


Ala Leu Leu Ser Leu Val Ser Leu Leu Ser Val Leu Leu Met Gly Cys 
            20                  25                  30          


Val Ala Glu Thr Gly Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala 
        35                  40                  45              


Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn 
    50                  55                  60                  


Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr 
65                  70                  75                  80  


Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe 
                85                  90                  95      


Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg 
            100                 105                 110         


Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys 
        115                 120                 125             


Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn 
    130                 135                 140                 


Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe 
145                 150                 155                 160 


Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile 
                165                 170                 175     


Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys 
            180                 185                 190         


Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly 
        195                 200                 205             


Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala 
    210                 215                 220                 


Pro Ala Thr Val Cys Gly Pro Gly Ser Gly Ser Gly Cys Lys Pro Cys 
225                 230                 235                 240 


Ile Cys Thr Val Pro Glu Val Ser Ser Val Phe Ile Phe Pro Pro Lys 
                245                 250                 255     


Pro Lys Asp Val Leu Thr Ile Thr Leu Thr Pro Lys Val Thr Cys Val 
            260                 265                 270         


Val Val Asp Ile Ser Lys Asp Asp Pro Glu Val Gln Phe Ser Trp Phe 
        275                 280                 285             


Val Asp Asp Val Glu Val His Thr Ala Gln Thr Gln Pro Arg Glu Glu 
    290                 295                 300                 


Gln Phe Asn Ser Thr Phe Arg Ser Val Ser Glu Leu Pro Ile Met His 
305                 310                 315                 320 


Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys Cys Arg Val Asn Ser Ala 
                325                 330                 335     


Ala Phe Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Arg 
            340                 345                 350         


Pro Lys Ala Pro Gln Val Tyr Thr Ile Pro Pro Pro Lys Glu Gln Met 
        355                 360                 365             


Ala Lys Asp Lys Val Ser Leu Thr Cys Met Ile Thr Asp Phe Phe Pro 
    370                 375                 380                 


Glu Asp Ile Thr Val Glu Trp Gln Trp Asn Gly Gln Pro Ala Glu Asn 
385                 390                 395                 400 


Tyr Lys Asn Thr Gln Pro Ile Met Asp Thr Asp Gly Ser Tyr Phe Val 
                405                 410                 415     


Tyr Ser Lys Leu Asn Val Gln Lys Ser Asn Trp Glu Ala Gly Asn Thr 
            420                 425                 430         


Phe Thr Cys Ser Val Leu His Glu Gly Leu His Asn His His Thr Glu 
        435                 440                 445             


Lys Ser Leu Ser His Ser Pro Gly Lys Leu Glu 
    450                 455                 


<210>  3
<211>  423
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Purified RBD-mouse IgG1 Fc-fusion monomer amino acid sequence

<400>  3

Glu Thr Gly Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
1               5                   10                  15      


Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
            20                  25                  30          


Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
        35                  40                  45              


Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
    50                  55                  60                  


Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
65                  70                  75                  80  


Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
                85                  90                  95      


Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
            100                 105                 110         


Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
        115                 120                 125             


Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
    130                 135                 140                 


Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
145                 150                 155                 160 


Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
                165                 170                 175     


Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
            180                 185                 190         


Thr Val Cys Gly Pro Gly Ser Gly Ser Gly Cys Lys Pro Cys Ile Cys 
        195                 200                 205             


Thr Val Pro Glu Val Ser Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 
    210                 215                 220                 


Asp Val Leu Thr Ile Thr Leu Thr Pro Lys Val Thr Cys Val Val Val 
225                 230                 235                 240 


Asp Ile Ser Lys Asp Asp Pro Glu Val Gln Phe Ser Trp Phe Val Asp 
                245                 250                 255     


Asp Val Glu Val His Thr Ala Gln Thr Gln Pro Arg Glu Glu Gln Phe 
            260                 265                 270         


Asn Ser Thr Phe Arg Ser Val Ser Glu Leu Pro Ile Met His Gln Asp 
        275                 280                 285             


Trp Leu Asn Gly Lys Glu Phe Lys Cys Arg Val Asn Ser Ala Ala Phe 
    290                 295                 300                 


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Arg Pro Lys 
305                 310                 315                 320 


Ala Pro Gln Val Tyr Thr Ile Pro Pro Pro Lys Glu Gln Met Ala Lys 
                325                 330                 335     


Asp Lys Val Ser Leu Thr Cys Met Ile Thr Asp Phe Phe Pro Glu Asp 
            340                 345                 350         


Ile Thr Val Glu Trp Gln Trp Asn Gly Gln Pro Ala Glu Asn Tyr Lys 
        355                 360                 365             


Asn Thr Gln Pro Ile Met Asp Thr Asp Gly Ser Tyr Phe Val Tyr Ser 
    370                 375                 380                 


Lys Leu Asn Val Gln Lys Ser Asn Trp Glu Ala Gly Asn Thr Phe Thr 
385                 390                 395                 400 


Cys Ser Val Leu His Glu Gly Leu His Asn His His Thr Glu Lys Ser 
                405                 410                 415     


Leu Ser His Ser Pro Gly Lys 
            420             


<210>  4
<211>  1395
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD-human IgG1 Fc-fusion nucleotide sequence

<400>  4
gaattcaagc ttgccaccat ggggatactt cccagccctg ggatgcctgc gctgctctcc       60

ctcgtgagcc ttctctccgt gctgctgatg ggttgcgtag ctgaagctag caatctgtgc      120

cccttcggcg aggtgttcaa cgccacaaga ttcgcctctg tgtacgcctg gaaccggaag      180

cggatcagca attgcgtggc cgactacagc gtgctgtaca acagcgccag cttcagcacc      240

ttcaagtgct acggcgtgtc ccctaccaag ctgaacgacc tgtgcttcac caacgtgtac      300

gccgacagct tcgtgatcag aggcgacgaa gtgcggcaga ttgcccctgg acagacaggc      360

aagatcgccg attacaacta caagctgccc gacgacttca ccggctgtgt gattgcctgg      420

aacagcaaca acctggacag caaagtcggc ggcaactaca actacctgta ccggctgttc      480

cgcaagagca acctgaagcc tttcgagcgg gacatcagca ccgaaatcta ccaggccggc      540

agcacccctt gcaatggcgt ggaaggcttc aactgctact tcccactgca aagctacggc      600

ttccagccta caaacggcgt gggctaccag ccttacagag tggtggtgct gagcttcgag      660

ctgctgcatg ctcctgccac agtgtgtgga cctggatccg gcagcggtac ccacacctgt      720

cctccatgtc ctgctccaga actgctcggc ggaccttccg tgttcctgtt tcctccaaag      780

cctaaggaca ccctgatgat cagcagaacc cctgaagtga cctgcgtggt ggtggatgtg      840

tctcacgagg accccgaagt gaagttcaat tggtacgtgg acggcgtgga agtgcacaac      900

gccaagacca agcctagaga ggaacagtac aacagcacct acagagtggt gtccgtgctg      960

accgtgctgc accaggattg gctgaacggc aaagagtaca agtgcaaggt gtccaacaag     1020

gccctgcctg ctcctatcga gaaaaccatc agcaaggcca agggccagcc tagggaaccc     1080

caggtttaca cactgcctcc aagccgggaa gagatgacca agaaccaggt gtccctgacc     1140

tgcctggtca agggctttta ccccagcgac attgccgtgg aatgggagag caatggccag     1200

cctgagaaca actacaagac cacacctcct gtgctggaca gcgacggctc attcttcctg     1260

tacagcaagc tgacagtgga caagagcaga tggcagcagg gcaacgtgtt cagctgctcc     1320

gtgatgcacg aggccctgca caaccactac acccagaagt ccctgagcct gtctcctggc     1380

aagtgatgac tcgag                                                      1395


<210>  5
<211>  461
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD-human IgG1 Fc-fusion amino acid  sequence

<400>  5

Glu Phe Lys Leu Ala Thr Met Gly Ile Leu Pro Ser Pro Gly Met Pro 
1               5                   10                  15      


Ala Leu Leu Ser Leu Val Ser Leu Leu Ser Val Leu Leu Met Gly Cys 
            20                  25                  30          


Val Ala Glu Ala Ser Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala 
        35                  40                  45              


Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn 
    50                  55                  60                  


Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr 
65                  70                  75                  80  


Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe 
                85                  90                  95      


Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg 
            100                 105                 110         


Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys 
        115                 120                 125             


Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn 
    130                 135                 140                 


Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe 
145                 150                 155                 160 


Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile 
                165                 170                 175     


Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys 
            180                 185                 190         


Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly 
        195                 200                 205             


Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala 
    210                 215                 220                 


Pro Ala Thr Val Cys Gly Pro Gly Ser Gly Ser Gly Thr His Thr Cys 
225                 230                 235                 240 


Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu 
                245                 250                 255     


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
            260                 265                 270         


Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys 
        275                 280                 285             


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
    290                 295                 300                 


Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
305                 310                 315                 320 


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
                325                 330                 335     


Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys 
            340                 345                 350         


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
        355                 360                 365             


Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
    370                 375                 380                 


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
385                 390                 395                 400 


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
                405                 410                 415     


Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln 
            420                 425                 430         


Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
        435                 440                 445             


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Leu Glu 
    450                 455                 460             


<210>  6
<211>  427
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD-human IgG1 Fc-fusion monomer amino acid sequence showing cleavage site

<400>  6

Glu Ala Ser Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
1               5                   10                  15      


Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
            20                  25                  30          


Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
        35                  40                  45              


Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
    50                  55                  60                  


Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
65                  70                  75                  80  


Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
                85                  90                  95      


Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
            100                 105                 110         


Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
        115                 120                 125             


Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
    130                 135                 140                 


Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
145                 150                 155                 160 


Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
                165                 170                 175     


Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
            180                 185                 190         


Thr Val Cys Gly Pro Gly Ser Gly Ser Gly Thr His Thr Cys Pro Pro 
        195                 200                 205             


Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro 
    210                 215                 220                 


Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr 
225                 230                 235                 240 


Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn 
                245                 250                 255     


Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg 
            260                 265                 270         


Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val 
        275                 280                 285             


Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser 
    290                 295                 300                 


Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys 
305                 310                 315                 320 


Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu 
                325                 330                 335     


Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe 
            340                 345                 350         


Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 
        355                 360                 365             


Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 
    370                 375                 380                 


Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly 
385                 390                 395                 400 


Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr 
                405                 410                 415     


Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
            420                 425         


<210>  7
<211>  729
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD monomer nucleotide sequence

<400>  7
gaattcaagc ttgccaccat ggggatactt cccagccctg ggatgcctgc gctgctctcc       60

ctcgtgagcc ttctctccgt gctgctgatg ggttgcgtag ctgaagctag caatctgtgc      120

cccttcggcg aggtgttcaa cgccacaaga ttcgcctctg tgtacgcctg gaaccggaag      180

cggatcagca attgcgtggc cgactacagc gtgctgtaca acagcgccag cttcagcacc      240

ttcaagtgct acggcgtgtc ccctaccaag ctgaacgacc tgtgcttcac caacgtgtac      300

gccgacagct tcgtgatcag aggcgacgaa gtgcggcaga ttgcccctgg acagacaggc      360

aagatcgccg attacaacta caagctgccc gacgacttca ccggctgtgt gattgcctgg      420

aacagcaaca acctggacag caaagtcggc ggcaactaca actacctgta ccggctgttc      480

cgcaagagca acctgaagcc tttcgagcgg gacatcagca ccgaaatcta ccaggccggc      540

agcacccctt gcaatggcgt ggaaggcttc aactgctact tcccactgca aagctacggc      600

ttccagccta caaacggcgt gggctaccag ccttacagag tggtggtgct gagcttcgag      660

ctgctgcatg ctcctgccac agtgtgtgga cctggatccc accaccacca tcaccactga      720

tgactcgag                                                              729


<210>  8
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD monomer amino acid sequence

<400>  8

Glu Phe Lys Leu Ala Thr Met Gly Ile Leu Pro Ser Pro Gly Met Pro 
1               5                   10                  15      


Ala Leu Leu Ser Leu Val Ser Leu Leu Ser Val Leu Leu Met Gly Cys 
            20                  25                  30          


Val Ala Glu Ala Ser Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala 
        35                  40                  45              


Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn 
    50                  55                  60                  


Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr 
65                  70                  75                  80  


Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe 
                85                  90                  95      


Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg 
            100                 105                 110         


Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys 
        115                 120                 125             


Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn 
    130                 135                 140                 


Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe 
145                 150                 155                 160 


Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile 
                165                 170                 175     


Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys 
            180                 185                 190         


Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly 
        195                 200                 205             


Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala 
    210                 215                 220                 


Pro Ala Thr Val Cys Gly Pro Gly Ser His His His His His His Leu 
225                 230                 235                 240 


Glu 
    


<210>  9
<211>  205
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD monomer amino acid sequence showing cleavage site

<400>  9

Glu Ala Ser Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
1               5                   10                  15      


Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
            20                  25                  30          


Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
        35                  40                  45              


Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
    50                  55                  60                  


Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
65                  70                  75                  80  


Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
                85                  90                  95      


Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
            100                 105                 110         


Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
        115                 120                 125             


Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
    130                 135                 140                 


Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
145                 150                 155                 160 


Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
                165                 170                 175     


Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
            180                 185                 190         


Thr Val Cys Gly Pro Gly Ser His His His His His His 
        195                 200                 205 


<210>  10
<211>  197
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD monomer amino acid sequence without His-tag showing cleavage site

<400>  10

Glu Ala Ser Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
1               5                   10                  15      


Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
            20                  25                  30          


Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
        35                  40                  45              


Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
    50                  55                  60                  


Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
65                  70                  75                  80  


Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
                85                  90                  95      


Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
            100                 105                 110         


Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
        115                 120                 125             


Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
    130                 135                 140                 


Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
145                 150                 155                 160 


Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
                165                 170                 175     


Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
            180                 185                 190         


Thr Val Cys Gly Pro 
        195         


<210>  11
<211>  1461
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD tandem dimer nucleotide sequence

<400>  11
gaattcaagc ttgccaccat ggggatactt cccagccctg ggatgcctgc gctgctctcc       60

ctcgtgagcc ttctctccgt gctgctgatg ggttgcgtag ctgaagctag cagagtgcag      120

cccaccgaga gcatcgtgcg gttccccaac atcaccaatc tgtgcccttt cggcgaggtg      180

ttcaacgcca ccagattcgc ctctgtgtac gcctggaacc ggaagcggat cagcaattgc      240

gtggccgact acagcgtgct gtacaacagc gccagcttca gcaccttcaa gtgctacggc      300

gtgtccccta ccaagctgaa cgacctgtgc ttcaccaacg tgtacgccga cagcttcgtg      360

atcagaggcg acgaagtgcg gcagattgcc cctggacaga caggcaagat cgccgattac      420

aactacaagc tgcccgacga cttcaccggc tgtgtgattg cctggaacag caacaacctg      480

gacagcaaag tcggcggcaa ctacaactac ctgtaccggc tgttccggaa gtccaacctg      540

aagcctttcg agcgggacat cagcaccgag atctatcagg ccggcagcac cccttgcaat      600

ggcgtggaag gcttcaactg ctacttccca ctgcagtcct acggcttcca gcctacaaac      660

ggcgtgggct accagcctta cagagtggtg gtgctgagct tcgagctgct gcatgctcct      720

gccacagtgt gcggccctaa gaaaagcacc aacctggtca agaacaagcg ggtgcagcca      780

acagagtcca ttgtgcgctt tccgaatatc accaacctct gtccattcgg ggaagtcttt      840

aatgccacac gcttcgccag cgtctacgct tggaatagga agagaatctc caactgcgtg      900

gcagattact ccgtcctgta caattccgcc agcttttcca cgtttaagtg ttatggcgtc      960

agccccacaa agctcaatga tctctgcttt accaatgtct atgccgactc ctttgtgatt     1020

cgcggagatg aagtccgcca gatcgctcca ggacagaccg gaaaaatcgc tgactataat     1080

tacaagctcc cggatgattt cacgggatgc gtgatcgctt ggaactccaa caatctggat     1140

tccaaagttg gcgggaatta caattatctc tatcgcctct tccgcaagag caatctgaag     1200

ccctttgaga gagacatctc tacagaaatc taccaggctg gctctacccc atgcaacggc     1260

gtcgaggggt ttaattgcta tttcccgctg caaagctacg ggtttcagcc caccaatggc     1320

gtcggatatc agccctatcg cgtggtggtc ctgtcttttg aactgctgca cgccccagct     1380

accgtgtgtg gcccaaaaaa gtccaccaat ctcgtgaaga acaaaggatc ccaccaccac     1440

catcaccact gatgactcga g                                               1461


<210>  12
<211>  483
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD tandem dimer amino acid sequence

<400>  12

Glu Phe Lys Leu Ala Thr Met Gly Ile Leu Pro Ser Pro Gly Met Pro 
1               5                   10                  15      


Ala Leu Leu Ser Leu Val Ser Leu Leu Ser Val Leu Leu Met Gly Cys 
            20                  25                  30          


Val Ala Glu Ala Ser Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe 
        35                  40                  45              


Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr 
    50                  55                  60                  


Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys 
65                  70                  75                  80  


Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe 
                85                  90                  95      


Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr 
            100                 105                 110         


Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln 
        115                 120                 125             


Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu 
    130                 135                 140                 


Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu 
145                 150                 155                 160 


Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg 
                165                 170                 175     


Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr 
            180                 185                 190         


Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr 
        195                 200                 205             


Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr 
    210                 215                 220                 


Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro 
225                 230                 235                 240 


Ala Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys 
                245                 250                 255     


Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn 
            260                 265                 270         


Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
        275                 280                 285             


Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
    290                 295                 300                 


Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
305                 310                 315                 320 


Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
                325                 330                 335     


Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
            340                 345                 350         


Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
        355                 360                 365             


Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
    370                 375                 380                 


Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
385                 390                 395                 400 


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
                405                 410                 415     


Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
            420                 425                 430         


Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
        435                 440                 445             


Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
    450                 455                 460                 


Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Gly Ser His His His 
465                 470                 475                 480 


His His His Leu Glu 
                485 


<210>  13
<211>  449
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD tandem dimer amino acid sequence showing cleavage site

<400>  13

Glu Ala Ser Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn 
1               5                   10                  15      


Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe 
            20                  25                  30          


Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala 
        35                  40                  45              


Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys 
    50                  55                  60                  


Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val 
65                  70                  75                  80  


Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala 
                85                  90                  95      


Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp 
            100                 105                 110         


Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser 
        115                 120                 125             


Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser 
    130                 135                 140                 


Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala 
145                 150                 155                 160 


Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro 
                165                 170                 175     


Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro 
            180                 185                 190         


Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr 
        195                 200                 205             


Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Arg Val 
    210                 215                 220                 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
225                 230                 235                 240 


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
                245                 250                 255     


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
            260                 265                 270         


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
        275                 280                 285             


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
    290                 295                 300                 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
305                 310                 315                 320 


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
                325                 330                 335     


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
            340                 345                 350         


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
        355                 360                 365             


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
    370                 375                 380                 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
385                 390                 395                 400 


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
                405                 410                 415     


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
            420                 425                 430         


Lys Ser Thr Asn Leu Val Lys Asn Lys Gly Ser His His His His His 
        435                 440                 445             


His 
    


<210>  14
<211>  441
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD tandem dimer amino acid sequence without His-tag showing cleavage site

<400>  14

Glu Ala Ser Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn 
1               5                   10                  15      


Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe 
            20                  25                  30          


Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala 
        35                  40                  45              


Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys 
    50                  55                  60                  


Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val 
65                  70                  75                  80  


Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala 
                85                  90                  95      


Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp 
            100                 105                 110         


Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser 
        115                 120                 125             


Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser 
    130                 135                 140                 


Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala 
145                 150                 155                 160 


Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro 
                165                 170                 175     


Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro 
            180                 185                 190         


Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr 
        195                 200                 205             


Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Arg Val 
    210                 215                 220                 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
225                 230                 235                 240 


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
                245                 250                 255     


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
            260                 265                 270         


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
        275                 280                 285             


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
    290                 295                 300                 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
305                 310                 315                 320 


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
                325                 330                 335     


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
            340                 345                 350         


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
        355                 360                 365             


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
    370                 375                 380                 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
385                 390                 395                 400 


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
                405                 410                 415     


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
            420                 425                 430         


Lys Ser Thr Asn Leu Val Lys Asn Lys 
        435                 440     


<210>  15
<211>  194
<212>  PRT
<213>  Severe acute respiratory syndrome coronavirus 2

<400>  15

Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
1               5                   10                  15      


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
            20                  25                  30          


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
        35                  40                  45              


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
    50                  55                  60                  


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
65                  70                  75                  80  


Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                85                  90                  95      


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
            100                 105                 110         


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
        115                 120                 125             


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
    130                 135                 140                 


Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
145                 150                 155                 160 


Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg 
                165                 170                 175     


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
            180                 185                 190         


Gly Pro 
        


<210>  16
<211>  221
<212>  PRT
<213>  Mus musculus

<400>  16

Cys Lys Pro Cys Ile Cys Thr Val Pro Glu Val Ser Ser Val Phe Ile 
1               5                   10                  15      


Phe Pro Pro Lys Pro Lys Asp Val Leu Thr Ile Thr Leu Thr Pro Lys 
            20                  25                  30          


Val Thr Cys Val Val Val Asp Ile Ser Lys Asp Asp Pro Glu Val Gln 
        35                  40                  45              


Phe Ser Trp Phe Val Asp Asp Val Glu Val His Thr Ala Gln Thr Gln 
    50                  55                  60                  


Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Ser Val Ser Glu Leu 
65                  70                  75                  80  


Pro Ile Met His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys Cys Arg 
                85                  90                  95      


Val Asn Ser Ala Ala Phe Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys 
            100                 105                 110         


Thr Lys Gly Arg Pro Lys Ala Pro Gln Val Tyr Thr Ile Pro Pro Pro 
        115                 120                 125             


Lys Glu Gln Met Ala Lys Asp Lys Val Ser Leu Thr Cys Met Ile Thr 
    130                 135                 140                 


Asp Phe Phe Pro Glu Asp Ile Thr Val Glu Trp Gln Trp Asn Gly Gln 
145                 150                 155                 160 


Pro Ala Glu Asn Tyr Lys Asn Thr Gln Pro Ile Met Asp Thr Asp Gly 
                165                 170                 175     


Ser Tyr Phe Val Tyr Ser Lys Leu Asn Val Gln Lys Ser Asn Trp Glu 
            180                 185                 190         


Ala Gly Asn Thr Phe Thr Cys Ser Val Leu His Glu Gly Leu His Asn 
        195                 200                 205             


His His Thr Glu Lys Ser Leu Ser His Ser Pro Gly Lys 
    210                 215                 220     


<210>  17
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker amino acid sequence

<400>  17

Gly Ser Gly Ser Gly 
1               5   


<210>  18
<211>  225
<212>  PRT
<213>  Homo sapiens

<400>  18

Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro 
1               5                   10                  15      


Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 
            20                  25                  30          


Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 
        35                  40                  45              


Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn 
    50                  55                  60                  


Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val 
65                  70                  75                  80  


Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu 
                85                  90                  95      


Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys 
            100                 105                 110         


Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
        115                 120                 125             


Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr 
    130                 135                 140                 


Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
145                 150                 155                 160 


Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
                165                 170                 175     


Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys 
            180                 185                 190         


Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
        195                 200                 205             


Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
    210                 215                 220                 


Lys 
225 


<210>  19
<211>  219
<212>  PRT
<213>  Severe acute respiratory syndrome coronavirus 2

<400>  19

Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn 
1               5                   10                  15      


Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
            20                  25                  30          


Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
        35                  40                  45              


Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
    50                  55                  60                  


Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
65                  70                  75                  80  


Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
                85                  90                  95      


Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
            100                 105                 110         


Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
        115                 120                 125             


Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
    130                 135                 140                 


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
145                 150                 155                 160 


Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
                165                 170                 175     


Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190         


Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
        195                 200                 205             


Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys 
    210                 215                 


<210>  20
<211>  219
<212>  PRT
<213>  Severe acute respiratory syndrome coronavirus 2

<400>  20

Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn 
1               5                   10                  15      


Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
            20                  25                  30          


Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
        35                  40                  45              


Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
    50                  55                  60                  


Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
65                  70                  75                  80  


Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
                85                  90                  95      


Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
            100                 105                 110         


Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
        115                 120                 125             


Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
    130                 135                 140                 


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
145                 150                 155                 160 


Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
                165                 170                 175     


Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190         


Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
        195                 200                 205             


Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys 
    210                 215                 


<210>  21
<211>  1273
<212>  PRT
<213>  Severe acute respiratory syndrome coronavirus 2

<400>  21

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  22
<211>  425
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Purified Beta RBD human IgG1 Fc-fusion monomer

<400>  22

Ser Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala 
1               5                   10                  15      


Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp 
            20                  25                  30          


Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr 
        35                  40                  45              


Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr 
    50                  55                  60                  


Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro 
65                  70                  75                  80  


Gly Gln Thr Gly Asn Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp 
                85                  90                  95      


Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys 
            100                 105                 110         


Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn 
        115                 120                 125             


Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly 
    130                 135                 140                 


Ser Thr Pro Cys Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu 
145                 150                 155                 160 


Gln Ser Tyr Gly Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr 
                165                 170                 175     


Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val 
            180                 185                 190         


Cys Gly Pro Gly Ser Gly Ser Gly Thr His Thr Cys Pro Pro Cys Pro 
        195                 200                 205             


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
    210                 215                 220                 


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
225                 230                 235                 240 


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
                245                 250                 255     


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
            260                 265                 270         


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
        275                 280                 285             


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
    290                 295                 300                 


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
305                 310                 315                 320 


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met 
                325                 330                 335     


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
            340                 345                 350         


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
        355                 360                 365             


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
    370                 375                 380                 


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
385                 390                 395                 400 


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
                405                 410                 415     


Lys Ser Leu Ser Leu Ser Pro Gly Lys 
            420                 425 


<210>  23
<211>  425
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD-human IgG1 Fc-fusion monomer amino acid sequence

<400>  23

Ser Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala 
1               5                   10                  15      


Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp 
            20                  25                  30          


Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr 
        35                  40                  45              


Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr 
    50                  55                  60                  


Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro 
65                  70                  75                  80  


Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp 
                85                  90                  95      


Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys 
            100                 105                 110         


Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn 
        115                 120                 125             


Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly 
    130                 135                 140                 


Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu 
145                 150                 155                 160 


Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr 
                165                 170                 175     


Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val 
            180                 185                 190         


Cys Gly Pro Gly Ser Gly Ser Gly Thr His Thr Cys Pro Pro Cys Pro 
        195                 200                 205             


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
    210                 215                 220                 


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
225                 230                 235                 240 


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
                245                 250                 255     


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
            260                 265                 270         


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
        275                 280                 285             


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
    290                 295                 300                 


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
305                 310                 315                 320 


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met 
                325                 330                 335     


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
            340                 345                 350         


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
        355                 360                 365             


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
    370                 375                 380                 


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
385                 390                 395                 400 


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
                405                 410                 415     


Lys Ser Leu Ser Leu Ser Pro Gly Lys 
            420                 425 


<210>  24
<211>  203
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD monomer amino acid sequence

<400>  24

Ser Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala 
1               5                   10                  15      


Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp 
            20                  25                  30          


Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr 
        35                  40                  45              


Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr 
    50                  55                  60                  


Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro 
65                  70                  75                  80  


Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp 
                85                  90                  95      


Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys 
            100                 105                 110         


Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn 
        115                 120                 125             


Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly 
    130                 135                 140                 


Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu 
145                 150                 155                 160 


Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr 
                165                 170                 175     


Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val 
            180                 185                 190         


Cys Gly Pro Gly Ser His His His His His His 
        195                 200             


<210>  25
<211>  195
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Purified RBD monomer amino acid sequence without His-tag

<400>  25

Ser Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala 
1               5                   10                  15      


Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp 
            20                  25                  30          


Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr 
        35                  40                  45              


Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr 
    50                  55                  60                  


Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro 
65                  70                  75                  80  


Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp 
                85                  90                  95      


Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys 
            100                 105                 110         


Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn 
        115                 120                 125             


Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly 
    130                 135                 140                 


Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu 
145                 150                 155                 160 


Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr 
                165                 170                 175     


Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val 
            180                 185                 190         


Cys Gly Pro 
        195 


<210>  26
<211>  447
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD tandem dimer amino acid sequence

<400>  26

Ser Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
1               5                   10                  15      


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
            20                  25                  30          


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
        35                  40                  45              


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
    50                  55                  60                  


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
65                  70                  75                  80  


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
                85                  90                  95      


Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
            100                 105                 110         


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
        115                 120                 125             


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
    130                 135                 140                 


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
145                 150                 155                 160 


Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
                165                 170                 175     


Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg 
            180                 185                 190         


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
        195                 200                 205             


Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Arg Val Gln Pro 
    210                 215                 220                 


Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe 
225                 230                 235                 240 


Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn 
                245                 250                 255     


Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn 
            260                 265                 270         


Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys 
        275                 280                 285             


Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile 
    290                 295                 300                 


Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile 
305                 310                 315                 320 


Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile 
                325                 330                 335     


Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn 
            340                 345                 350         


Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg 
        355                 360                 365             


Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly 
    370                 375                 380                 


Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln 
385                 390                 395                 400 


Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser 
                405                 410                 415     


Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser 
            420                 425                 430         


Thr Asn Leu Val Lys Asn Lys Gly Ser His His His His His His 
        435                 440                 445         


<210>  27
<211>  439
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD tandem dimer amino acid sequence without His-tag

<400>  27

Ser Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
1               5                   10                  15      


Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
            20                  25                  30          


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
        35                  40                  45              


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
    50                  55                  60                  


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
65                  70                  75                  80  


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
                85                  90                  95      


Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
            100                 105                 110         


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
        115                 120                 125             


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
    130                 135                 140                 


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
145                 150                 155                 160 


Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
                165                 170                 175     


Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg 
            180                 185                 190         


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
        195                 200                 205             


Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Arg Val Gln Pro 
    210                 215                 220                 


Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe 
225                 230                 235                 240 


Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn 
                245                 250                 255     


Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn 
            260                 265                 270         


Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys 
        275                 280                 285             


Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile 
    290                 295                 300                 


Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile 
305                 310                 315                 320 


Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile 
                325                 330                 335     


Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn 
            340                 345                 350         


Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg 
        355                 360                 365             


Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly 
    370                 375                 380                 


Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln 
385                 390                 395                 400 


Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser 
                405                 410                 415     


Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser 
            420                 425                 430         


Thr Asn Leu Val Lys Asn Lys 
        435                 


<210>  28
<211>  194
<212>  PRT
<213>  Severe acute respiratory syndrome coronavirus 2

<400>  28

Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
1               5                   10                  15      


Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
            20                  25                  30          


Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
        35                  40                  45              


Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
    50                  55                  60                  


Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
65                  70                  75                  80  


Gln Thr Gly Asn Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
                85                  90                  95      


Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
            100                 105                 110         


Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
        115                 120                 125             


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
    130                 135                 140                 


Thr Pro Cys Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
145                 150                 155                 160 


Ser Tyr Gly Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg 
                165                 170                 175     


Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
            180                 185                 190         


Gly Pro 
        


<210>  29
<211>  1344
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Beta RBD-human IgG1 Fc-fusion nucleotide sequence with leader 
       sequence

<400>  29
atgcctgcgc tgctctccct cgtgagcctt ctctccgtgc tgctgatggg ttgcgtagct       60

gaagctagca atctgtgccc cttcggcgag gtgttcaacg ccacaagatt cgcctctgtg      120

tacgcctgga accggaagcg gatcagcaat tgcgtggccg actacagcgt gctgtacaac      180

agcgccagct tcagcacctt caagtgctac ggcgtgtccc ctaccaagct gaacgacctg      240

tgcttcacca acgtgtacgc cgacagcttc gtgatcagag gcgacgaagt gcggcagatt      300

gcccctggac agacaggcaa catcgccgat tacaactaca agctgcccga cgacttcacc      360

ggctgtgtga ttgcctggaa cagcaacaac ctggacagca aagtcggcgg caactacaac      420

tacctgtacc ggctgttccg caagagcaac ctgaagcctt tcgagcggga catcagcacc      480

gaaatctacc aggccggcag caccccttgc aatggcgtga aaggcttcaa ctgctacttc      540

ccactgcaaa gctacggctt ccagcctaca tacggcgtgg gctaccagcc ttacagagtg      600

gtggtgctga gcttcgagct gctgcatgct cctgccacag tgtgtggacc tggatccggc      660

agcggtaccc acacctgtcc tccatgtcct gctccagaac tgctcggcgg accttccgtg      720

ttcctgtttc ctccaaagcc taaggacacc ctgatgatca gcagaacccc tgaagtgacc      780

tgcgtggtgg tggatgtgtc tcacgaggac cccgaagtga agttcaattg gtacgtggac      840

ggcgtggaag tgcacaacgc caagaccaag cctagagagg aacagtacaa cagcacctac      900

agagtggtgt ccgtgctgac cgtgctgcac caggattggc tgaacggcaa agagtacaag      960

tgcaaggtgt ccaacaaggc cctgcctgct cctatcgaga aaaccatcag caaggccaag     1020

ggccagccta gggaacccca ggtttacaca ctgcctccaa gccgggaaga gatgaccaag     1080

aaccaggtgt ccctgacctg cctggtcaag ggcttttacc ccagcgacat tgccgtggaa     1140

tgggagagca atggccagcc tgagaacaac tacaagacca cacctcctgt gctggacagc     1200

gacggctcat tcttcctgta cagcaagctg acagtggaca agagcagatg gcagcagggc     1260

aacgtgttca gctgctccgt gatgcacgag gccctgcaca accactacac ccagaagtcc     1320

ctgagcctgt ctcctggcaa gtga                                            1344


<210>  30
<211>  1278
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Beta RBD-human IgG1 Fc-fusion nucleotide sequence without leader 
       sequence

<400>  30
agcaatctgt gccccttcgg cgaggtgttc aacgccacaa gattcgcctc tgtgtacgcc       60

tggaaccgga agcggatcag caattgcgtg gccgactaca gcgtgctgta caacagcgcc      120

agcttcagca ccttcaagtg ctacggcgtg tcccctacca agctgaacga cctgtgcttc      180

accaacgtgt acgccgacag cttcgtgatc agaggcgacg aagtgcggca gattgcccct      240

ggacagacag gcaacatcgc cgattacaac tacaagctgc ccgacgactt caccggctgt      300

gtgattgcct ggaacagcaa caacctggac agcaaagtcg gcggcaacta caactacctg      360

taccggctgt tccgcaagag caacctgaag cctttcgagc gggacatcag caccgaaatc      420

taccaggccg gcagcacccc ttgcaatggc gtgaaaggct tcaactgcta cttcccactg      480

caaagctacg gcttccagcc tacatacggc gtgggctacc agccttacag agtggtggtg      540

ctgagcttcg agctgctgca tgctcctgcc acagtgtgtg gacctggatc cggcagcggt      600

acccacacct gtcctccatg tcctgctcca gaactgctcg gcggaccttc cgtgttcctg      660

tttcctccaa agcctaagga caccctgatg atcagcagaa cccctgaagt gacctgcgtg      720

gtggtggatg tgtctcacga ggaccccgaa gtgaagttca attggtacgt ggacggcgtg      780

gaagtgcaca acgccaagac caagcctaga gaggaacagt acaacagcac ctacagagtg      840

gtgtccgtgc tgaccgtgct gcaccaggat tggctgaacg gcaaagagta caagtgcaag      900

gtgtccaaca aggccctgcc tgctcctatc gagaaaacca tcagcaaggc caagggccag      960

cctagggaac cccaggttta cacactgcct ccaagccggg aagagatgac caagaaccag     1020

gtgtccctga cctgcctggt caagggcttt taccccagcg acattgccgt ggaatgggag     1080

agcaatggcc agcctgagaa caactacaag accacacctc ctgtgctgga cagcgacggc     1140

tcattcttcc tgtacagcaa gctgacagtg gacaagagca gatggcagca gggcaacgtg     1200

ttcagctgct ccgtgatgca cgaggccctg cacaaccact acacccagaa gtccctgagc     1260

ctgtctcctg gcaagtga                                                   1278


<210>  31
<211>  1344
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD-human IgG1 Fc-fusion nucleotide sequence with leader sequence

<400>  31
atgcctgcgc tgctctccct cgtgagcctt ctctccgtgc tgctgatggg ttgcgtagct       60

gaagctagca atctgtgccc cttcggcgag gtgttcaacg ccacaagatt cgcctctgtg      120

tacgcctgga accggaagcg gatcagcaat tgcgtggccg actacagcgt gctgtacaac      180

agcgccagct tcagcacctt caagtgctac ggcgtgtccc ctaccaagct gaacgacctg      240

tgcttcacca acgtgtacgc cgacagcttc gtgatcagag gcgacgaagt gcggcagatt      300

gcccctggac agacaggcaa gatcgccgat tacaactaca agctgcccga cgacttcacc      360

ggctgtgtga ttgcctggaa cagcaacaac ctggacagca aagtcggcgg caactacaac      420

tacctgtacc ggctgttccg caagagcaac ctgaagcctt tcgagcggga catcagcacc      480

gaaatctacc aggccggcag caccccttgc aatggcgtgg aaggcttcaa ctgctacttc      540

ccactgcaaa gctacggctt ccagcctaca aacggcgtgg gctaccagcc ttacagagtg      600

gtggtgctga gcttcgagct gctgcatgct cctgccacag tgtgtggacc tggatccggc      660

agcggtaccc acacctgtcc tccatgtcct gctccagaac tgctcggcgg accttccgtg      720

ttcctgtttc ctccaaagcc taaggacacc ctgatgatca gcagaacccc tgaagtgacc      780

tgcgtggtgg tggatgtgtc tcacgaggac cccgaagtga agttcaattg gtacgtggac      840

ggcgtggaag tgcacaacgc caagaccaag cctagagagg aacagtacaa cagcacctac      900

agagtggtgt ccgtgctgac cgtgctgcac caggattggc tgaacggcaa agagtacaag      960

tgcaaggtgt ccaacaaggc cctgcctgct cctatcgaga aaaccatcag caaggccaag     1020

ggccagccta gggaacccca ggtttacaca ctgcctccaa gccgggaaga gatgaccaag     1080

aaccaggtgt ccctgacctg cctggtcaag ggcttttacc ccagcgacat tgccgtggaa     1140

tgggagagca atggccagcc tgagaacaac tacaagacca cacctcctgt gctggacagc     1200

gacggctcat tcttcctgta cagcaagctg acagtggaca agagcagatg gcagcagggc     1260

aacgtgttca gctgctccgt gatgcacgag gccctgcaca accactacac ccagaagtcc     1320

ctgagcctgt ctcctggcaa gtga                                            1344


<210>  32
<211>  1278
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD-human IgG1 Fc-fusion nucleotide sequence without leader 
       sequence

<400>  32
agcaatctgt gccccttcgg cgaggtgttc aacgccacaa gattcgcctc tgtgtacgcc       60

tggaaccgga agcggatcag caattgcgtg gccgactaca gcgtgctgta caacagcgcc      120

agcttcagca ccttcaagtg ctacggcgtg tcccctacca agctgaacga cctgtgcttc      180

accaacgtgt acgccgacag cttcgtgatc agaggcgacg aagtgcggca gattgcccct      240

ggacagacag gcaagatcgc cgattacaac tacaagctgc ccgacgactt caccggctgt      300

gtgattgcct ggaacagcaa caacctggac agcaaagtcg gcggcaacta caactacctg      360

taccggctgt tccgcaagag caacctgaag cctttcgagc gggacatcag caccgaaatc      420

taccaggccg gcagcacccc ttgcaatggc gtggaaggct tcaactgcta cttcccactg      480

caaagctacg gcttccagcc tacaaacggc gtgggctacc agccttacag agtggtggtg      540

ctgagcttcg agctgctgca tgctcctgcc acagtgtgtg gacctggatc cggcagcggt      600

acccacacct gtcctccatg tcctgctcca gaactgctcg gcggaccttc cgtgttcctg      660

tttcctccaa agcctaagga caccctgatg atcagcagaa cccctgaagt gacctgcgtg      720

gtggtggatg tgtctcacga ggaccccgaa gtgaagttca attggtacgt ggacggcgtg      780

gaagtgcaca acgccaagac caagcctaga gaggaacagt acaacagcac ctacagagtg      840

gtgtccgtgc tgaccgtgct gcaccaggat tggctgaacg gcaaagagta caagtgcaag      900

gtgtccaaca aggccctgcc tgctcctatc gagaaaacca tcagcaaggc caagggccag      960

cctagggaac cccaggttta cacactgcct ccaagccggg aagagatgac caagaaccag     1020

gtgtccctga cctgcctggt caagggcttt taccccagcg acattgccgt ggaatgggag     1080

agcaatggcc agcctgagaa caactacaag accacacctc ctgtgctgga cagcgacggc     1140

tcattcttcc tgtacagcaa gctgacagtg gacaagagca gatggcagca gggcaacgtg     1200

ttcagctgct ccgtgatgca cgaggccctg cacaaccact acacccagaa gtccctgagc     1260

ctgtctcctg gcaagtga                                                   1278


<210>  33
<211>  455
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Beta RBD human IgG1 Fc-fusion monomer

<400>  33

Met Gly Ile Leu Pro Ser Pro Gly Met Pro Ala Leu Leu Ser Leu Val 
1               5                   10                  15      


Ser Leu Leu Ser Val Leu Leu Met Gly Cys Val Ala Glu Ala Ser Asn 
            20                  25                  30          


Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
        35                  40                  45              


Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
    50                  55                  60                  


Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
65                  70                  75                  80  


Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
                85                  90                  95      


Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
            100                 105                 110         


Thr Gly Asn Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
        115                 120                 125             


Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
    130                 135                 140                 


Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
145                 150                 155                 160 


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
                165                 170                 175     


Pro Cys Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
            180                 185                 190         


Tyr Gly Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val 
        195                 200                 205             


Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
    210                 215                 220                 


Pro Gly Ser Gly Ser Gly Thr His Thr Cys Pro Pro Cys Pro Ala Pro 
225                 230                 235                 240 


Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
                245                 250                 255     


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
            260                 265                 270         


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
        275                 280                 285             


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
    290                 295                 300                 


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
305                 310                 315                 320 


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
                325                 330                 335     


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
            340                 345                 350         


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys 
        355                 360                 365             


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
    370                 375                 380                 


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
385                 390                 395                 400 


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
                405                 410                 415     


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
            420                 425                 430         


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
        435                 440                 445             


Leu Ser Leu Ser Pro Gly Lys 
    450                 455 


