                         SEQUENCE LISTING

<110>  BASF SE
 
<120>  BIOCONVERSION OF FERULIC ACID TO VANILLIN

<130>  074008-2034-WO-000324

<150>  US 63/127,449
<151>  2020-12-18

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  305
<212>  DNA
<213>  Amycolatopsis sp. ATCC 39116


<220>
<221>  misc_feature
<222>  (1)..(305)
<223>  Nucleic Acid Sequence of promoter region of ech-fcs operon

<400>  1
gcccccgatt gtccgcaccg gccgcggccg ccgggaaggc gccgcccggt cgcggcaacc       60

gcgctcaccg gcgcgtcgcg cccgagcccg tcaccctcag tccgctggag gcgcgggcgg      120

cggtgcagtc agcgccgaac ggcctgttca ccgggctcgg cggttcctgt ccgaggcgct      180

gctgctggcc gcgctcggcg ggctggtcgc cgccgtcctg atcggcgcgc cggccatctg      240

accttgacgc cgtcggcccg ctcttgctat ccctatatca gaactactga tatagggagc      300

gatgc                                                                  305


<210>  2
<211>  678
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<222>  (1)..(678)
<223>  Nucleic Acid Sequence of red fluorescent protein - codon 
       optimized for Amycolatopsis

<220>
<221>  misc_feature
<222>  (676)..(678)
<223>  stop codon

<400>  2
atggccagca gcgaggatgt gatcaaggag tttatgcgct tcaaggtgcg catggagggg       60

tccgtgaacg gccacgagtt cgagatcgag ggtgagggcg agggccgccc gtacgagggg      120

acccagaccg caaagctgaa ggtcaccaag ggcggccccc tgcccttcgc gtgggacatc      180

ctgagcccac agttccagta cggctccaag gcgtacgtca agcacccggc ggacatcccc      240

gactacctga agctgagctt cccggagggc ttcaagtggg agcgggtcat gaacttcgag      300

gacgggggcg tggtcacggt gacgcaggac agctcgttgc aggatggcga gttcatctac      360

aaggtgaagc tgcgcggcac gaacttcccc agcgacggcc ccgtgatgca gaaaaagacg      420

atgggctggg aggcgtccac cgagaggatg tacccggagg acggcgccct gaagggcgag      480

atcaagatgc ggctgaaact gaaggacgga ggccactacg acgcggaggt caagacgacg      540

tacatggcga agaagcccgt gcagcttccg ggcgcataca agacggacat caagctggac      600

atcacgtccc acaacgagga ctatacgatc gtcgagcagt acgagcgggc ggagggccgc      660

cactccacgg gcgcgtga                                                    678


<210>  3
<211>  137
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<222>  (1)..(137)
<223>  Nucleic Acid sequence of terminator for reporter gene

<400>  3
aggtccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc       60

tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct      120

ttctgcgttt atagctt                                                     137


<210>  4
<211>  483
<212>  DNA
<213>  Amycolatopsis sp. ATCC 39116


<220>
<221>  misc_feature
<222>  (1)..(483)
<223>  Nucleic acid sequence of echR (repressor of ech-fcs operon)

<220>
<221>  misc_feature
<222>  (481)..(483)
<223>  stop codon

<400>  4
gtggtgaccg aatcccgcgc cgaggacgcc ccgctgaccc tctacctggt caagcggctg       60

gagctggtga tccgctcgct gatggacgac gcgctgcgcc cgttcgggct gaccaccctg      120

cagtacaccg cgctgaccgc gctgcggcac cgcaacgggc tgtcgtccgc gcagctcgcg      180

cgccgctcgt tcgtccggcc ccagaccatg cacaccatgg tgctcacgct ggagaagtac      240

gggctcatcg agcgcgcgga ggacccggcc aaccgccggg tcctgctcgc caccctcacc      300

gagcgcggca agcaggtcct cgacgagtgc acgccgctgg tccgggagct cgaagaccgg      360

atgctctccg gcatggacga cgaccgccgc gccgggttcc gccgggacct ggaggacggc      420

tacggcatgc tcgcctcgca cgccaacgct cagcgcgcgt tgacgaacgg cggcggcgag      480

taa                                                                    483


<210>  5
<211>  160
<212>  PRT
<213>  Amycolatopsis sp. ATCC 39116


<220>
<221>  MISC_FEATURE
<222>  (1)..(160)
<223>  Amino Acid Sequence of EchR (repressor of ech-fcs operon)

<400>  5

Met Val Thr Glu Ser Arg Ala Glu Asp Ala Pro Leu Thr Leu Tyr Leu 
1               5                   10                  15      


Val Lys Arg Leu Glu Leu Val Ile Arg Ser Leu Met Asp Asp Ala Leu 
            20                  25                  30          


Arg Pro Phe Gly Leu Thr Thr Leu Gln Tyr Thr Ala Leu Thr Ala Leu 
        35                  40                  45              


Arg His Arg Asn Gly Leu Ser Ser Ala Gln Leu Ala Arg Arg Ser Phe 
    50                  55                  60                  


Val Arg Pro Gln Thr Met His Thr Met Val Leu Thr Leu Glu Lys Tyr 
65                  70                  75                  80  


Gly Leu Ile Glu Arg Ala Glu Asp Pro Ala Asn Arg Arg Val Leu Leu 
                85                  90                  95      


Ala Thr Leu Thr Glu Arg Gly Lys Gln Val Leu Asp Glu Cys Thr Pro 
            100                 105                 110         


Leu Val Arg Glu Leu Glu Asp Arg Met Leu Ser Gly Met Asp Asp Asp 
        115                 120                 125             


Arg Arg Ala Gly Phe Arg Arg Asp Leu Glu Asp Gly Tyr Gly Met Leu 
    130                 135                 140                 


Ala Ser His Ala Asn Ala Gln Arg Ala Leu Thr Asn Gly Gly Gly Glu 
145                 150                 155                 160 


<210>  6
<211>  1461
<212>  DNA
<213>  Amycolatopsis sp. ATCC 39116


<220>
<221>  misc_feature
<222>  (1)..(1461)
<223>  Nucleic acid sequence of vdh (Vanillin dehydrogenase)

<220>
<221>  misc_feature
<222>  (1459)..(1461)
<223>  stop codon

<400>  6
gtgagctttc tcgacgacga gaagtggacc ggacgcgtct tcaccggcag ctgggagcgc       60

gcggcgggcg gcgacgcggc cgtcatcgag cccgcgaccg gcgacgaact ggggcgcgtc      120

ggcatcgcct cgccccagga cctggcggcc tccgcggcca aggcggccga ggcgcagcgc      180

gcctgggcgg cgacctcctt ccaagaacgc gccgcggtcc tgcgccgcgc cggcgacctg      240

tggcagcagc acgccgccga gctgaaggac tggctgatcc gcgagtcggg cagcatcccc      300

ggcaaggccg acttcgaact gcacgtcgcc gcgcaggagt gctacgaggc cgccgcgctg      360

ccctcccacc cgacgggtga ggtcctgccg agcgaggcgc cgcggctgag catggcccgc      420

cgcgtgcccg ccggcgtggt cggcgtgatc gcgccgttca acgcgccgct gatcctgtcg      480

atccgctcgg tcgcgccggc gctggcgctg ggcaacagcg tcgtgctcaa gccggacccc      540

cgcaccgcgg tctgcggtgg cgtggcgctg gccagggtct tcgaggaggc cgggctgccc      600

gccggggtcc tgcacgtgct gccgggcggc ccggacgtcg gcgccgcgct ggtcgaggac      660

aagcacgtcc gcgtcatctc gttcaccgga tcgaccgccg cgggccgcgc ggtcggcgag      720

tccgcgggcc gccacctcaa gcgcgcccac ctggaactgg gcggcaactc ggcgctgatc      780

gtgctcgacg acgccgacct ggagcaggcg atgagcgccg ccgcgtgggg ctcgttcttc      840

caccagggcc agatctgcat gaccaccggg cggcacctgg tgcacgcctc actctacgac      900

gaatacgtgg accgcctggc ggacaaggcc agccacctgc cggtgggcaa cccgttcacc      960

gagcaggtcg cgctcggccc gatcatcgac gccaagcagc gcgacaagat ccacggcctg     1020

gtgacgtcca gtgtggacgc cggcgcgaag gtcgccgcgg gcggcaccta cgaggacctc     1080

ttctaccgcg ccaccgtgct cgccggcgcg ggcccctcgg tgcccgccta cgaccaggag     1140

gtgttcggcc cggtcgcccc ggtcgcgaag ttcaccagcc tggacgaggc cgcgaagctc     1200

gcgtcggaga gcgagtacgg gctgtcgctg ggcatcatca ccgcggacgt ggcgaaggga     1260

ctggcgctgg ccgaccgcat cccgaccggc atcgcgcaca tcaacgacca gacggtcaac     1320

gacgaggcgc tggccccgtt cggcggcgtg ttcgactccg gcaccggctc ccgcttcggc     1380

gggccggccg cgaacatcga ggcgttcacc gagacccgct gggtcacgat gcgcggcgac     1440

gtcgccggct acccgttctg a                                               1461


<210>  7
<211>  486
<212>  PRT
<213>  Amycolatopsis sp. ATCC 39116


<220>
<221>  MISC_FEATURE
<222>  (1)..(486)
<223>  Amino acid sequence of Vdh (Vanillin dehydrogenase) protein

<400>  7

Val Ser Phe Leu Asp Asp Glu Lys Trp Thr Gly Arg Val Phe Thr Gly 
1               5                   10                  15      


Ser Trp Glu Arg Ala Ala Gly Gly Asp Ala Ala Val Ile Glu Pro Ala 
            20                  25                  30          


Thr Gly Asp Glu Leu Gly Arg Val Gly Ile Ala Ser Pro Gln Asp Leu 
        35                  40                  45              


Ala Ala Ser Ala Ala Lys Ala Ala Glu Ala Gln Arg Ala Trp Ala Ala 
    50                  55                  60                  


Thr Ser Phe Gln Glu Arg Ala Ala Val Leu Arg Arg Ala Gly Asp Leu 
65                  70                  75                  80  


Trp Gln Gln His Ala Ala Glu Leu Lys Asp Trp Leu Ile Arg Glu Ser 
                85                  90                  95      


Gly Ser Ile Pro Gly Lys Ala Asp Phe Glu Leu His Val Ala Ala Gln 
            100                 105                 110         


Glu Cys Tyr Glu Ala Ala Ala Leu Pro Ser His Pro Thr Gly Glu Val 
        115                 120                 125             


Leu Pro Ser Glu Ala Pro Arg Leu Ser Met Ala Arg Arg Val Pro Ala 
    130                 135                 140                 


Gly Val Val Gly Val Ile Ala Pro Phe Asn Ala Pro Leu Ile Leu Ser 
145                 150                 155                 160 


Ile Arg Ser Val Ala Pro Ala Leu Ala Leu Gly Asn Ser Val Val Leu 
                165                 170                 175     


Lys Pro Asp Pro Arg Thr Ala Val Cys Gly Gly Val Ala Leu Ala Arg 
            180                 185                 190         


Val Phe Glu Glu Ala Gly Leu Pro Ala Gly Val Leu His Val Leu Pro 
        195                 200                 205             


Gly Gly Pro Asp Val Gly Ala Ala Leu Val Glu Asp Lys His Val Arg 
    210                 215                 220                 


Val Ile Ser Phe Thr Gly Ser Thr Ala Ala Gly Arg Ala Val Gly Glu 
225                 230                 235                 240 


Ser Ala Gly Arg His Leu Lys Arg Ala His Leu Glu Leu Gly Gly Asn 
                245                 250                 255     


Ser Ala Leu Ile Val Leu Asp Asp Ala Asp Leu Glu Gln Ala Met Ser 
            260                 265                 270         


Ala Ala Ala Trp Gly Ser Phe Phe His Gln Gly Gln Ile Cys Met Thr 
        275                 280                 285             


Thr Gly Arg His Leu Val His Ala Ser Leu Tyr Asp Glu Tyr Val Asp 
    290                 295                 300                 


Arg Leu Ala Asp Lys Ala Ser His Leu Pro Val Gly Asn Pro Phe Thr 
305                 310                 315                 320 


Glu Gln Val Ala Leu Gly Pro Ile Ile Asp Ala Lys Gln Arg Asp Lys 
                325                 330                 335     


Ile His Gly Leu Val Thr Ser Ser Val Asp Ala Gly Ala Lys Val Ala 
            340                 345                 350         


Ala Gly Gly Thr Tyr Glu Asp Leu Phe Tyr Arg Ala Thr Val Leu Ala 
        355                 360                 365             


Gly Ala Gly Pro Ser Val Pro Ala Tyr Asp Gln Glu Val Phe Gly Pro 
    370                 375                 380                 


Val Ala Pro Val Ala Lys Phe Thr Ser Leu Asp Glu Ala Ala Lys Leu 
385                 390                 395                 400 


Ala Ser Glu Ser Glu Tyr Gly Leu Ser Leu Gly Ile Ile Thr Ala Asp 
                405                 410                 415     


Val Ala Lys Gly Leu Ala Leu Ala Asp Arg Ile Pro Thr Gly Ile Ala 
            420                 425                 430         


His Ile Asn Asp Gln Thr Val Asn Asp Glu Ala Leu Ala Pro Phe Gly 
        435                 440                 445             


Gly Val Phe Asp Ser Gly Thr Gly Ser Arg Phe Gly Gly Pro Ala Ala 
    450                 455                 460                 


Asn Ile Glu Ala Phe Thr Glu Thr Arg Trp Val Thr Met Arg Gly Asp 
465                 470                 475                 480 


Val Ala Gly Tyr Pro Phe 
                485     


