                         SEQUENCE LISTING

<110>  BASF SE
 
<120>  BIOSYNTHESIS OF VANILLIN FROM ISOEUGENOL

<130>  074008-2041-WO-000323

<150>  US 63/127,487
<151>  2020-12-18

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  1656
<212>  DNA
<213>  Violaceomyces paulstris


<220>
<221>  misc_feature
<222>  (1)..(1656)
<223>  Nucleic Acid Sequence of VpIEM

<220>
<221>  misc_feature
<222>  (1654)..(1656)
<223>  stop codon

<400>  1
atggctcccg cacctacagt ccaagatgcg gcaccggtcg ccgtcgccgt gccgagcaag       60

gccactaaca agggatcgtt cgtccacccc accgacatcc ttccaagcgg atggcctact      120

gcgaccgacc tctctggagg agctcagcca cgtcgtttcg aaggaaccat ttacgacgtc      180

atggttcgag ggaccatccc caaggagctc cacgggacct tttaccgcat catgccagac      240

tacgccgaag caccaaccta ctacaaagga ggagagctca acgctcccat tgacggtgat      300

ggtaccgtcg ccgctttccg cttcaaggac ggcaacgtcg actaccgcca gcgcttcgta      360

gagaccgacc gcttcaaggt ggagaggagg gcgaggaaga gcatgtatgg cctctaccgc      420

aatccttaca cccaccaccc ttgcgtccga cagacggtcg actcgaccgc caacaccaac      480

gtcgtcatgc acgccggccg cttcctcgcc atgaaggaga atggcaacgc ctacgagctg      540

gaccctcaca cgctcaagac gctcggctac aacccattca agctgccctc caagaccatg      600

accgcccatc caaagcagga tgccgtcact ggtaacctcg tcggcttcgg ttacgaggcc      660

aaaggactgg cgaccaagga cgtctactac ttcgaggtcg acacccaggg caacatcgtt      720

cacgacctct ggctcgaggc cccctggtgc gccttcatcc acgactgtgc cctcaccccc      780

aactatctcg tcttgatgct ctggccgttc gaggccgaca tcgagcgcat gaaggccggt      840

ggacaccatt gggcctacga ctacgacaag cccatcacct ggatcaccat cccgcgtgga      900

gccaagagca aggacgaggt aaagtactgg tactggaaga acggaatgcc gatccacacg      960

gcggcggggt acgaggacga gaagggacgc atcatcatcg acagctcgct cgtccacggc     1020

aacgccttcc cattcttccc acccgactcg gaggagcagc gaaagcgtca ggaggcggat     1080

ggaactccga tcgcccagtt cgtccgatgg acgatcgacc cgagcaagga ccccaacgag     1140

aggctccccg atccagaggt ggtcctcgac actccctccg agttccccca gatcgacaac     1200

cgattcatgg gcaaggaata ctcgagcgcc ttcatcaatg tcttcatgcc cgatcgatcc     1260

gacacgggca agaacgtctt ccaaggcctc aacggcctgt gtcactaccg tcggaaggag     1320

ggcactgccg atttctacta tgcaggtgac aactgcctga tccaggagcc cgtcttcagt     1380

ccaaggtcca aggacgcccc cgagggcgat ggcttcgtct tggccatcgt cgatcggctc     1440

gacgccaacc gaagcgaagt agtcatcatt gacacgcgag acttcaccaa agcggtcgct     1500

gccgttcaac ttccgttcgc catccgatcc ggcattcacg gccagtggat ccagggcaac     1560

acgatccccg atttcgacac ccgcggcctc gtcgaccttc ccaaggagga gcactgggcg     1620

cctccagaag caagtgccta cgatccaaac atgtga                               1656


<210>  2
<211>  551
<212>  PRT
<213>  Violaceomyces paulstris


<220>
<221>  MISC_FEATURE
<222>  (1)..(551)
<223>  Amino Acid Sequence of VpIEM

<400>  2

Met Ala Pro Ala Pro Thr Val Gln Asp Ala Ala Pro Val Ala Val Ala 
1               5                   10                  15      


Val Pro Ser Lys Ala Thr Asn Lys Gly Ser Phe Val His Pro Thr Asp 
            20                  25                  30          


Ile Leu Pro Ser Gly Trp Pro Thr Ala Thr Asp Leu Ser Gly Gly Ala 
        35                  40                  45              


Gln Pro Arg Arg Phe Glu Gly Thr Ile Tyr Asp Val Met Val Arg Gly 
    50                  55                  60                  


Thr Ile Pro Lys Glu Leu His Gly Thr Phe Tyr Arg Ile Met Pro Asp 
65                  70                  75                  80  


Tyr Ala Glu Ala Pro Thr Tyr Tyr Lys Gly Gly Glu Leu Asn Ala Pro 
                85                  90                  95      


Ile Asp Gly Asp Gly Thr Val Ala Ala Phe Arg Phe Lys Asp Gly Asn 
            100                 105                 110         


Val Asp Tyr Arg Gln Arg Phe Val Glu Thr Asp Arg Phe Lys Val Glu 
        115                 120                 125             


Arg Arg Ala Arg Lys Ser Met Tyr Gly Leu Tyr Arg Asn Pro Tyr Thr 
    130                 135                 140                 


His His Pro Cys Val Arg Gln Thr Val Asp Ser Thr Ala Asn Thr Asn 
145                 150                 155                 160 


Val Val Met His Ala Gly Arg Phe Leu Ala Met Lys Glu Asn Gly Asn 
                165                 170                 175     


Ala Tyr Glu Leu Asp Pro His Thr Leu Lys Thr Leu Gly Tyr Asn Pro 
            180                 185                 190         


Phe Lys Leu Pro Ser Lys Thr Met Thr Ala His Pro Lys Gln Asp Ala 
        195                 200                 205             


Val Thr Gly Asn Leu Val Gly Phe Gly Tyr Glu Ala Lys Gly Leu Ala 
    210                 215                 220                 


Thr Lys Asp Val Tyr Tyr Phe Glu Val Asp Thr Gln Gly Asn Ile Val 
225                 230                 235                 240 


His Asp Leu Trp Leu Glu Ala Pro Trp Cys Ala Phe Ile His Asp Cys 
                245                 250                 255     


Ala Leu Thr Pro Asn Tyr Leu Val Leu Met Leu Trp Pro Phe Glu Ala 
            260                 265                 270         


Asp Ile Glu Arg Met Lys Ala Gly Gly His His Trp Ala Tyr Asp Tyr 
        275                 280                 285             


Asp Lys Pro Ile Thr Trp Ile Thr Ile Pro Arg Gly Ala Lys Ser Lys 
    290                 295                 300                 


Asp Glu Val Lys Tyr Trp Tyr Trp Lys Asn Gly Met Pro Ile His Thr 
305                 310                 315                 320 


Ala Ala Gly Tyr Glu Asp Glu Lys Gly Arg Ile Ile Ile Asp Ser Ser 
                325                 330                 335     


Leu Val His Gly Asn Ala Phe Pro Phe Phe Pro Pro Asp Ser Glu Glu 
            340                 345                 350         


Gln Arg Lys Arg Gln Glu Ala Asp Gly Thr Pro Ile Ala Gln Phe Val 
        355                 360                 365             


Arg Trp Thr Ile Asp Pro Ser Lys Asp Pro Asn Glu Arg Leu Pro Asp 
    370                 375                 380                 


Pro Glu Val Val Leu Asp Thr Pro Ser Glu Phe Pro Gln Ile Asp Asn 
385                 390                 395                 400 


Arg Phe Met Gly Lys Glu Tyr Ser Ser Ala Phe Ile Asn Val Phe Met 
                405                 410                 415     


Pro Asp Arg Ser Asp Thr Gly Lys Asn Val Phe Gln Gly Leu Asn Gly 
            420                 425                 430         


Leu Cys His Tyr Arg Arg Lys Glu Gly Thr Ala Asp Phe Tyr Tyr Ala 
        435                 440                 445             


Gly Asp Asn Cys Leu Ile Gln Glu Pro Val Phe Ser Pro Arg Ser Lys 
    450                 455                 460                 


Asp Ala Pro Glu Gly Asp Gly Phe Val Leu Ala Ile Val Asp Arg Leu 
465                 470                 475                 480 


Asp Ala Asn Arg Ser Glu Val Val Ile Ile Asp Thr Arg Asp Phe Thr 
                485                 490                 495     


Lys Ala Val Ala Ala Val Gln Leu Pro Phe Ala Ile Arg Ser Gly Ile 
            500                 505                 510         


His Gly Gln Trp Ile Gln Gly Asn Thr Ile Pro Asp Phe Asp Thr Arg 
        515                 520                 525             


Gly Leu Val Asp Leu Pro Lys Glu Glu His Trp Ala Pro Pro Glu Ala 
    530                 535                 540                 


Ser Ala Tyr Asp Pro Asn Met 
545                 550     


<210>  3
<211>  1656
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<222>  (1)..(1656)
<223>  Nucleic Acid Sequence of VpIEM codon optimized for expression in 
       Escherichia coli

<220>
<221>  misc_feature
<222>  (1654)..(1656)
<223>  stop codon

<400>  3
atggcaccgg cccctaccgt tcaggatgca gcaccggttg cagtggccgt tccgagtaaa       60

gccaccaata aaggcagctt tgtgcatccg accgatattc tgccgagtgg ctggccgacc      120

gccaccgatc tgagtggtgg cgcccagccg cgtcgctttg aaggcaccat ttatgatgtg      180

atggtgcgtg gtacaattcc gaaagaactg catggcacct tttatcgtat tatgccggat      240

tatgccgaag caccgaccta ttataaaggt ggcgaactga atgccccgat tgatggcgat      300

ggcaccgttg ccgcatttcg ttttaaagat ggtaatgtgg attaccgcca gcgctttgtt      360

gaaaccgatc gttttaaagt tgaacgtcgc gcccgcaaaa gtatgtatgg tctgtatcgt      420

aatccgtata cccatcatcc gtgcgtgcgt cagaccgttg atagtaccgc aaataccaat      480

gttgtgatgc atgcaggccg ctttctggcc atgaaagaaa atggtaatgc atacgaactg      540

gaccctcata ccctgaaaac cctgggctat aatccgttta aattaccgag taaaaccatg      600

accgcccatc cgaaacagga tgccgtgacc ggtaatctgg ttggctttgg ctatgaagcc      660

aaaggcttag ccaccaaaga tgtttattat tttgaggtgg atacccaggg caatattgtt      720

catgatctgt ggctggaagc accgtggtgt gcatttattc atgattgcgc actgaccccg      780

aattatctgg ttctgatgct gtggccgttt gaagcagata ttgaacgcat gaaagcaggc      840

ggtcatcatt gggcatacga ttatgataaa ccgattacct ggattaccat tccgcgtggt      900

gcaaaaagca aagatgaagt gaaatattgg tactggaaaa atggcatgcc gattcatacc      960

gcagccggct atgaagatga aaaaggccgc attattattg atagcagcct ggttcatggc     1020

aatgcctttc cgttttttcc gccggatagc gaagaacagc gtaaacgtca ggaagcagat     1080

ggcaccccga ttgcacagtt tgttcgctgg accattgatc cgagcaaaga tccgaatgaa     1140

cgcctgccgg accctgaagt tgttctggat accccgagcg aatttccgca gattgataat     1200

cgctttatgg gcaaagaata tagtagcgcc tttattaatg tgtttatgcc ggatcgcagt     1260

gataccggca aaaatgtttt tcagggcctg aatggcctgt gtcattatcg tcgcaaagaa     1320

ggcaccgcag atttttatta tgccggtgat aattgcctga ttcaggaacc ggtttttagt     1380

ccgcgtagta aagatgcacc ggaaggcgat ggctttgtgc tggcaattgt tgatcgtctg     1440

gatgccaatc gtagtgaagt ggtgattatt gatacccgcg attttaccaa agccgtggcc     1500

gcagtgcagc tgccgtttgc cattcgtagt ggcattcatg gccagtggat tcagggcaat     1560

accattcctg attttgatac ccgtggcctg gtggatctgc cgaaagaaga acattgggca     1620

ccgccggaag ccagtgccta tgatccgaat atgtaa                               1656


<210>  4
<211>  6949
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<222>  (1)..(6949)
<223>  Nucleic Acid Sequence of VpIEM-pET28a

<400>  4
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg       60

cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc      120

ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg      180

gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc      240

acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt      300

ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc      360

ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta      420

acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt      480

tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta      540

tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat      600

tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa      660

actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc      720

gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga      780

aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc      840

agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac      900

cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac      960

aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat     1020

tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag     1080

tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca     1140

taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac     1200

ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg     1260

tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca     1320

tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac     1380

cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa     1440

cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga     1500

gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg     1560

gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc     1620

agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag     1680

aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc     1740

agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg     1800

cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac     1860

accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga     1920

aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt     1980

ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag     2040

cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg     2100

gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta     2160

tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc     2220

agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg     2280

tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta     2340

caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg     2400

ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct     2460

gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag     2520

gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc     2580

gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag     2640

aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt     2700

ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa     2760

acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg     2820

ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg     2880

tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc     2940

tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta     3000

cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca     3060

gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc     3120

ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc     3180

catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa     3240

ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc     3300

gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac     3360

gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca     3420

ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta     3480

atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa     3540

cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat     3600

tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca     3660

ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa     3720

aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt     3780

atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg     3840

cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca     3900

gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta     3960

tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg     4020

agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat     4080

gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct     4140

ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg     4200

catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat     4260

tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc     4320

tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca     4380

gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg     4440

ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt     4500

tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg     4560

catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct     4620

cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga     4680

tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg     4740

ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc     4800

ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg     4860

cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg     4920

gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga     4980

aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa     5040

ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac     5100

agcagcggcc tggtgccgcg cggcagccat atggcaccgg cccctaccgt tcaggatgca     5160

gcaccggttg cagtggccgt tccgagtaaa gccaccaata aaggcagctt tgtgcatccg     5220

accgatattc tgccgagtgg ctggccgacc gccaccgatc tgagtggtgg cgcccagccg     5280

cgtcgctttg aaggcaccat ttatgatgtg atggtgcgtg gtacaattcc gaaagaactg     5340

catggcacct tttatcgtat tatgccggat tatgccgaag caccgaccta ttataaaggt     5400

ggcgaactga atgccccgat tgatggcgat ggcaccgttg ccgcatttcg ttttaaagat     5460

ggtaatgtgg attaccgcca gcgctttgtt gaaaccgatc gttttaaagt tgaacgtcgc     5520

gcccgcaaaa gtatgtatgg tctgtatcgt aatccgtata cccatcatcc gtgcgtgcgt     5580

cagaccgttg atagtaccgc aaataccaat gttgtgatgc atgcaggccg ctttctggcc     5640

atgaaagaaa atggtaatgc atacgaactg gaccctcata ccctgaaaac cctgggctat     5700

aatccgttta aattaccgag taaaaccatg accgcccatc cgaaacagga tgccgtgacc     5760

ggtaatctgg ttggctttgg ctatgaagcc aaaggcttag ccaccaaaga tgtttattat     5820

tttgaggtgg atacccaggg caatattgtt catgatctgt ggctggaagc accgtggtgt     5880

gcatttattc atgattgcgc actgaccccg aattatctgg ttctgatgct gtggccgttt     5940

gaagcagata ttgaacgcat gaaagcaggc ggtcatcatt gggcatacga ttatgataaa     6000

ccgattacct ggattaccat tccgcgtggt gcaaaaagca aagatgaagt gaaatattgg     6060

tactggaaaa atggcatgcc gattcatacc gcagccggct atgaagatga aaaaggccgc     6120

attattattg atagcagcct ggttcatggc aatgcctttc cgttttttcc gccggatagc     6180

gaagaacagc gtaaacgtca ggaagcagat ggcaccccga ttgcacagtt tgttcgctgg     6240

accattgatc cgagcaaaga tccgaatgaa cgcctgccgg accctgaagt tgttctggat     6300

accccgagcg aatttccgca gattgataat cgctttatgg gcaaagaata tagtagcgcc     6360

tttattaatg tgtttatgcc ggatcgcagt gataccggca aaaatgtttt tcagggcctg     6420

aatggcctgt gtcattatcg tcgcaaagaa ggcaccgcag atttttatta tgccggtgat     6480

aattgcctga ttcaggaacc ggtttttagt ccgcgtagta aagatgcacc ggaaggcgat     6540

ggctttgtgc tggcaattgt tgatcgtctg gatgccaatc gtagtgaagt ggtgattatt     6600

gatacccgcg attttaccaa agccgtggcc gcagtgcagc tgccgtttgc cattcgtagt     6660

ggcattcatg gccagtggat tcagggcaat accattcctg attttgatac ccgtggcctg     6720

gtggatctgc cgaaagaaga acattgggca ccgccggaag ccagtgccta tgatccgaat     6780

atgtaactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag     6840

gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct     6900

aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggat                 6949


<210>  5
<211>  3952
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<222>  (1)..(3952)
<223>  Nucleic Acid Sequence of VpIEM-pUVAP

<400>  5
ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc atcgtttagg       60

caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggaatt gtgagcggat      120

aacaatttca actataagaa ggagatatac atatgtccaa gacacaggag ttcaggcctt      180

tgacactgcc acccaagctg tcgttaagtg acttcaatga gttcatccag gatattattc      240

gaatcgttgg ctctgaaaat gttgaagtca ttagctcgaa ggaccagatt gttgacggtt      300

cttatatgaa acctacgcac acgcacgatc cccatcatgt catggaccag gactacttcc      360

ttgcctcagc aattgttgct cctcgcaatg tcgccgatgt gcagtcgatt gtcggacttg      420

ccaataagtt ctcatttccc ctctggccca tctctattgg aagaaattcc ggatatggcg      480

gtgctgcgcc acgggttagt ggcagtgtcg tgctggacat gggaaagaat atgaacagag      540

ttctggaagt gaacgtggaa ggcgcatatt gcgtggtgga gcccggtgta acttaccacg      600

acttgcataa ttaccttgag gcgaacaatc ttcgagacaa attatggctt gatgtaccgg      660

atcttggtgg cggttctgtt ctcggcaatg ccgttgagag aggtgtgggc tatacgcctt      720

acggagatca ttggatgatg cacagtggga tggaagtcgt ccttgcgaat ggcgagcttc      780

ttaggactgg catgggggct ctacctgatc ctaaacgtcc cgaaacgatg gggctaaagc      840

cagaagacca gccatggagc aaaatcgctc atctgtttcc ttatggcttc ggtccctata      900

tagatgggct attcagccaa tcgaatatgg gaattgttac caagatcggg atctggttaa      960

tgcccaatcc agggggttat caatcctact tgatcacact acccaaagat ggtgatttaa     1020

aacaagccgt cgatattatt cgtccccttc gtctaggcat ggcccttcaa aatgttccca     1080

ctattcgcca cattcttttg gatgcagcgg tgctcggtga caagcgatct tattcatcca     1140

agaccgaacc cctctccgac gaggaattag acaagatcgc gaaacagctc aacttgggac     1200

gatggaactt ttacggggcg ctctatggac ctgagccgat tcgaagggtt ctctgggaaa     1260

cgattaaaga cgcattctcg gcgatcccag gcgtcaagtt ttattttccg gaggacactc     1320

ctgaaaactc cgttctccgc gtgcgtgata agactatgca aggcattcca acttacgacg     1380

agctaaagtg gatcgactgg ctccctaatg gtgcgcatct gttcttctct cctattgcga     1440

aggtatctgg tgaagatgca atgatgcaat acgcagtcac caagaaaagg tgtcaggagg     1500

ctgggttaga ttttatcggc actttcacag tcggtatgag agagatgcat catatcgttt     1560

gtattgtgtt caacaagaag gacctaatac aaaagagaaa agtacagtgg ctgatgagaa     1620

cccttattga tgactgtgct gcaaatggat ggggcgaata tcgaacccat ctggccttca     1680

tggaccaaat tatggaaacc tacaactgga acaacagcag cttcctaagg ttcaatgagg     1740

tcctcaagaa tgcggtggac cctaatggca tcattgcccc gggaaagtct ggtgtttggc     1800

cgagtcaata cagtcatgtt acttggaaac tgtaagcggc cgccaccgct gagcaataac     1860

tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa     1920

ctatatccgg gtaacgaatt caagcttgat atcattcagg acgagcctca gactccagcg     1980

taactggact gcaatcaact cactggctca ccttcacggg tgggcctttc ttcggtagaa     2040

aatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa     2100

aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc     2160

gaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag     2220

ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg     2280

ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga     2340

tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc     2400

ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc     2460

acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga     2520

gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt     2580

cgccacctct gacttgagca tcgatttttg tgatgctcgt caggggggcg gagcctatgg     2640

aaaaacgcca gcaacgcaga aaggcccacc cgaaggtgag ccaggtgatt acatttgggc     2700

cctcatcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca     2760

tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg     2820

agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt     2880

ttttcctgtt tggtcattta ccaatgctta atcagtgagg cacctatctc agcgatctgt     2940

ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag     3000

ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca     3060

gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact     3120

ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca     3180

gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg     3240

tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc     3300

atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg     3360

gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca     3420

tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt     3480

atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc     3540

agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc     3600

ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca     3660

tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa     3720

aagggaataa gggcgacacg gaaatgttga atactcatag ctcctgaaaa tctcgataac     3780

tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg     3840

tgccgatcaa gtcaaaagcc tccggtcgga ggcttttgac tttctgctat ggaggtcagg     3900

tatgatttaa atggtcagta ttgagcgata tctagagaat tcgtcaaacc cc             3952


<210>  6
<211>  28
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<222>  (1)..(28)
<223>  Nucleic acid sequence of the Primer VpIEM01

<400>  6
catatggcac cggcccctac cgttcagg                                          28


<210>  7
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<222>  (1)..(32)
<223>  Nucleic acid sequence of the Primer VpIEM02

<400>  7
ctcgagttac atattcggat cataggcact gg                                     32


<210>  8
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<222>  (1)..(50)
<223>  Nucleic acid sequence of the Primer VpIEM03

<400>  8
actataagaa ggagatatac atatgggcag cagccatcat catcatcatc                  50


<210>  9
<211>  44
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<222>  (1)..(44)
<223>  Nucleic acid sequence of the Primer VpIEM04

<400>  9
cagcggtggc ggccgcttac atattcggat cataggcact ggct                        44


<210>  10
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<222>  (1)..(32)
<223>  Nucleic acid sequence of the Primer VpIEM05

<400>  10
taagcggccg ccaccgctga gcaataacta gc                                     32


<210>  11
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<222>  (1)..(40)
<223>  Nucleic acid sequence of the Primer VpIEM06

<400>  11
catatgtata tctccttctt atagttgaaa ttgttatccg                             40


