                         SEQUENCE LISTING

<110>  Eli Lilly and Company
 
<120>  DUAL AMYLIN AND CALCITONIN RECEPTOR AGONISTS AND USES THEREOF

<130>  X22758

<150>  US 63/127,186
<151>  2020-12-18

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  N-terminus is acetylated

<220>
<221>  MISC_FEATURE
<222>  (14)..(14)
<223>  Xaa at position 14 is Aib

<220>
<221>  MOD_RES
<222>  (32)..(32)
<223>  C-terminus is amidated

<400>  1

Ala Ser His Leu Ser Thr Ala Val Leu Gly Lys Leu Ser Xaa Glu Leu 
1               5                   10                  15      


His Lys Leu Glu Asp Tyr Pro Arg Thr Asp Val Gly Ala Glu Ser Pro 
            20                  25                  30          


<210>  2
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  N-terminus is acetylated

<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  The Lys at position 11 is chemically modified by conjugation of 
       the epsilon amino group of the Lys sidechain with 
       2-[2-(2-Amino-ethoxy)-ethoxy]-acetyl)2-(gamma-Glu)-CO-(CH2)18-CO2
       H

<220>
<221>  MISC_FEATURE
<222>  (14)..(14)
<223>  Xaa at position 14 is Aib

<220>
<221>  MOD_RES
<222>  (32)..(32)
<223>  C-terminus is amidated

<400>  2

Ala Ser His Leu Ser Thr Ala Val Leu Gly Lys Leu Ser Xaa Glu Leu 
1               5                   10                  15      


His Lys Leu Glu Asp Tyr Pro Arg Thr Asp Val Gly Ala Glu Ser Pro 
            20                  25                  30          


<210>  3
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  DISULFID
<222>  (2)..(7)

<220>
<221>  MOD_RES
<222>  (37)..(37)
<223>  C-terminus is amidated

<400>  3

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Asn Asn Phe Gly Pro Ile Leu Pro Pro Thr Asn Val 
            20                  25                  30          


Gly Ser Asn Thr Tyr 
        35          


<210>  4
<211>  32
<212>  PRT
<213>  Homo sapiens


<220>
<221>  DISULFID
<222>  (1)..(7)

<220>
<221>  MOD_RES
<222>  (32)..(32)
<223>  C-terminus is amidated

<400>  4

Cys Gly Asn Leu Ser Thr Cys Met Leu Gly Thr Tyr Thr Gln Asp Phe 
1               5                   10                  15      


Asn Lys Phe His Thr Phe Pro Gln Thr Ala Ile Gly Val Gly Ala Pro 
            20                  25                  30          


<210>  5
<211>  37
<212>  PRT
<213>  Homo sapiens


<220>
<221>  DISULFID
<222>  (2)..(7)

<220>
<221>  MOD_RES
<222>  (37)..(37)
<223>  C-terminus is amidated

<400>  5

Lys Cys Asn Thr Ala Thr Cys Ala Thr Gln Arg Leu Ala Asn Phe Leu 
1               5                   10                  15      


Val His Ser Ser Asn Asn Phe Gly Ala Ile Leu Ser Ser Thr Asn Val 
            20                  25                  30          


Gly Ser Asn Thr Tyr 
        35          


<210>  6
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Aib

<220>
<221>  MOD_RES
<222>  (20)..(20)
<223>  The Lys at position 20 is chemically modified by conjugation of 
       the epsilon amino group of the Lys sidechain with 
       2-[2-(2-Amino-ethoxy)-ethoxy]-acetyl)2-(gamma-Glu)-CO-(CH2)16-CO2
       H

<400>  6

His Xaa Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
            20                  25                  30      


<210>  7
<211>  34
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Aib

<220>
<221>  MOD_RES
<222>  (20)..(20)
<223>  The Lys at position 20 is chemically modified by conjugation of 
       the epsilon amino group of the Lys sidechain with 
       2-[2-(2-Amino-ethoxy)-ethoxy]-acetyl)2-(gamma-Glu)-CO-(CH2)18-CO2
       H

<220>
<221>  MOD_RES
<222>  (34)..(34)
<223>  C-terminus is amidated

<400>  7

His Xaa Gln Gly Thr Phe Thr Ser Asp Tyr Ser Lys Tyr Leu Asp Glu 
1               5                   10                  15      


Lys Lys Ala Lys Glu Phe Val Glu Trp Leu Leu Glu Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly 
        


<210>  8
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Aib

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  Xaa at position 13 is alpha-methyl-Leu

<220>
<221>  MOD_RES
<222>  (17)..(17)
<223>  The Lys at position 17 is chemically modified by conjugation of 
       the epsilon amino group of the Lys sidechain with 
       2-[2-(2-Amino-ethoxy)-ethoxy]-acetyl)-(gamma-Glu)-CO-(CH2)18-CO2H

<220>
<221>  MISC_FEATURE
<222>  (20)..(20)
<223>  Xaa at position 20 is Aib

<220>
<221>  MOD_RES
<222>  (39)..(39)
<223>  C-terminus is amidated

<400>  8

Tyr Xaa Gln Gly Thr Phe Thr Ser Asp Tyr Ser Ile Xaa Leu Asp Lys 
1               5                   10                  15      


Lys Ala Gln Xaa Ala Phe Ile Glu Tyr Leu Leu Glu Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly Ala Pro Pro Pro Ser 
        35                  


<210>  9
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Aib

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  Xaa at position 13 is Aib

<220>
<221>  MOD_RES
<222>  (20)..(20)
<223>  The Lys at position 20 is chemically modified by conjugation of 
       the epsilon amino group of the Lys sidechain with 
       2-[2-(2-Amino-ethoxy)-ethoxy]-acetyl)2-(gamma-Glu)2-CO-(CH2)18-CO
       2H

<220>
<221>  MISC_FEATURE
<222>  (22)..(22)
<223>  Xaa at position 22 is 1Nal

<220>
<221>  MOD_RES
<222>  (39)..(39)
<223>  C-terminus is amidated

<400>  9

Tyr Xaa Glu Gly Thr Phe Thr Ser Asp Tyr Ser Ile Xaa Leu Asp Lys 
1               5                   10                  15      


Ile Ala Gln Lys Ala Xaa Val Gln Trp Leu Ile Ala Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly Ala Pro Pro Pro Ser 
        35                  


<210>  10
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Aib

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  Xaa at position 13 is Aib

<220>
<221>  MOD_RES
<222>  (20)..(20)
<223>  The Lys at position 20 is chemically modified by conjugation of 
       the epsilon amino group of the Lys sidechain with 
       2-[2-(2-Amino-ethoxy)-ethoxy]-acetyl)2-(gamma-Glu)-CO-(CH2)18-CO2
       H

<220>
<221>  MOD_RES
<222>  (39)..(39)
<223>  C-terminus is amidated

<400>  10

Tyr Xaa Glu Gly Thr Phe Thr Ser Asp Tyr Ser Ile Xaa Leu Asp Lys 
1               5                   10                  15      


Ile Ala Gln Lys Ala Phe Val Gln Trp Leu Ile Ala Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly Ala Pro Pro Pro Ser 
        35                  


<210>  11
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MOD_RES
<222>  (44)..(44)
<223>  C-terminus is amidated

<400>  11

His Gly Glu Gly Thr Phe Thr Ser Asp Leu Ser Lys Gln Met Glu Glu 
1               5                   10                  15      


Glu Ala Val Arg Leu Phe Ile Glu Trp Leu Lys Asn Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly Ala Pro Pro Ser Lys Lys Lys Lys Lys Lys 
        35                  40                  


<210>  12
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MOD_RES
<222>  (20)..(20)
<223>  The Lys at position 20 is chemically modified by conjugation of 
       the epsilon amino group of the Lys sidechain with 
       -(gamma-Glu)-CO-(CH2)14-CH3

<400>  12

His Ala Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
            20                  25                  30      


<210>  13
<211>  275
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  13

His Gly Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Glu 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Gly Gly Gly Gly 
            20                  25                  30          


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Glu 
        35                  40                  45              


Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala 
    50                  55                  60                  


Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
65                  70                  75                  80  


Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
                85                  90                  95      


Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu 
            100                 105                 110         


Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr 
        115                 120                 125             


Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 
    130                 135                 140                 


Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser 
145                 150                 155                 160 


Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln 
                165                 170                 175     


Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val 
            180                 185                 190         


Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
        195                 200                 205             


Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
    210                 215                 220                 


Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr 
225                 230                 235                 240 


Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val 
                245                 250                 255     


Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
            260                 265                 270         


Ser Leu Gly 
        275 


