                         SEQUENCE LISTING

<110>  BASF Corporation
 
<120>  Method of starch processing

<130>  191315WO01

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  436
<212>  PRT
<213>  artificial

<220>
<223>  alpha-amylase

<400>  1

Met Ala Lys Tyr Ser Glu Leu Glu Lys Gly Gly Val Ile Met Gln Ala 
1               5                   10                  15      


Phe Tyr Trp Asp Val Pro Ser Gly Gly Ile Trp Trp Asp Thr Ile Arg 
            20                  25                  30          


Gln Lys Ile Pro Glu Trp Tyr Asp Ala Gly Ile Ser Ala Ile Trp Ile 
        35                  40                  45              


Pro Pro Ala Ser Lys Gly Met Gly Gly Ala Tyr Ser Met Gly Tyr Asp 
    50                  55                  60                  


Pro Tyr Asp Phe Phe Asp Leu Gly Glu Tyr Asp Gln Lys Gly Thr Val 
65                  70                  75                  80  


Glu Thr Arg Phe Gly Ser Lys Gln Glu Leu Val Asn Met Ile Asn Thr 
                85                  90                  95      


Ala His Ala Tyr Gly Met Lys Val Ile Ala Asp Ile Val Ile Asn His 
            100                 105                 110         


Arg Ala Gly Gly Asp Leu Glu Trp Asn Pro Phe Val Asn Asp Tyr Thr 
        115                 120                 125             


Trp Thr Asp Phe Ser Lys Val Ala Ser Gly Lys Tyr Thr Ala Asn Tyr 
    130                 135                 140                 


Leu Asp Phe His Pro Asn Glu Leu His Ala Gly Asp Ser Gly Thr Phe 
145                 150                 155                 160 


Gly Gly Tyr Pro Asp Ile Cys His Asp Lys Ser Trp Asp Gln Tyr Trp 
                165                 170                 175     


Leu Trp Ala Ser Gln Glu Ser Tyr Ala Ala Tyr Leu Arg Ser Ile Gly 
            180                 185                 190         


Ile Asp Ala Trp Arg Phe Asp Tyr Val Lys Gly Tyr Ala Pro Trp Val 
        195                 200                 205             


Val Lys Asp Trp Leu Asn Trp Trp Gly Gly Trp Ala Val Gly Glu Tyr 
    210                 215                 220                 


Trp Asp Thr Asn Val Asp Ala Val Leu Asn Trp Ala Tyr Ser Ser Gly 
225                 230                 235                 240 


Ala Lys Val Phe Asp Phe Ala Leu Tyr Tyr Lys Met Asp Glu Ala Phe 
                245                 250                 255     


Asp Asn Lys Asn Ile Pro Ala Leu Val Ser Ala Leu Gln Asn Gly Gln 
            260                 265                 270         


Thr Val Val Ser Arg Asp Pro Phe Lys Ala Val Thr Phe Val Ala Asn 
        275                 280                 285             


His Asp Thr Asp Ile Ile Trp Asn Lys Tyr Pro Ala Tyr Ala Phe Ile 
    290                 295                 300                 


Leu Thr Tyr Glu Gly Gln Pro Thr Ile Phe Tyr Arg Asp Tyr Glu Glu 
305                 310                 315                 320 


Trp Leu Asn Lys Asp Lys Leu Lys Asn Leu Ile Trp Ile His Glu Asn 
                325                 330                 335     


Leu Ala Gly Gly Ser Thr Asp Ile Val Tyr Tyr Asp Asn Asp Glu Leu 
            340                 345                 350         


Ile Phe Val Arg Asn Gly Tyr Gly Asp Lys Pro Gly Leu Ile Thr Tyr 
        355                 360                 365             


Ile Asn Leu Gly Ser Ser Lys Ala Gly Arg Trp Val Tyr Val Pro Lys 
    370                 375                 380                 


Phe Ala Gly Ala Cys Ile His Glu Tyr Thr Gly Asn Leu Gly Gly Trp 
385                 390                 395                 400 


Val Asp Lys Tyr Val Tyr Ser Ser Gly Trp Val Tyr Leu Glu Ala Pro 
                405                 410                 415     


Ala Tyr Asp Pro Ala Asn Gly Gln Tyr Gly Tyr Ser Val Trp Ser Tyr 
            420                 425                 430         


Cys Gly Val Gly 
        435     


<210>  2
<211>  549
<212>  PRT
<213>  Geobacillus stearothermophilus

<400>  2

Met Leu Thr Phe His Arg Ile Ile Arg Lys Gly Trp Met Phe Leu Leu 
1               5                   10                  15      


Ala Phe Leu Leu Thr Ala Leu Leu Phe Cys Pro Thr Gly Gln Pro Ala 
            20                  25                  30          


Lys Ala Ala Ala Pro Phe Asn Gly Thr Met Met Gln Tyr Phe Glu Trp 
        35                  40                  45              


Tyr Leu Pro Asp Asp Gly Thr Leu Trp Thr Lys Val Ala Asn Glu Ala 
    50                  55                  60                  


Asn Asn Leu Ser Ser Leu Gly Ile Thr Ala Leu Trp Leu Pro Pro Ala 
65                  70                  75                  80  


Tyr Lys Gly Thr Ser Arg Ser Asp Val Gly Tyr Gly Val Tyr Asp Leu 
                85                  90                  95      


Tyr Asp Leu Gly Glu Phe Asn Gln Lys Gly Ala Val Arg Thr Lys Tyr 
            100                 105                 110         


Gly Thr Lys Ala Gln Tyr Leu Gln Ala Ile Gln Ala Ala His Ala Ala 
        115                 120                 125             


Gly Met Gln Val Tyr Ala Asp Val Val Phe Asp His Lys Gly Gly Ala 
    130                 135                 140                 


Asp Gly Thr Glu Trp Val Asp Ala Val Glu Val Asn Pro Ser Asp Arg 
145                 150                 155                 160 


Asn Gln Glu Ile Ser Gly Thr Tyr Gln Ile Gln Ala Trp Thr Lys Phe 
                165                 170                 175     


Asp Phe Pro Gly Arg Gly Asn Thr Tyr Ser Ser Phe Lys Trp Arg Trp 
            180                 185                 190         


Tyr His Phe Asp Gly Val Asp Trp Asp Glu Ser Arg Lys Leu Ser Arg 
        195                 200                 205             


Ile Tyr Lys Phe Arg Gly Ile Gly Lys Ala Trp Asp Trp Glu Val Asp 
    210                 215                 220                 


Thr Glu Asn Gly Asn Tyr Asp Tyr Leu Met Tyr Ala Asp Leu Asp Met 
225                 230                 235                 240 


Asp His Pro Glu Val Val Thr Glu Leu Lys Ser Trp Gly Lys Trp Tyr 
                245                 250                 255     


Val Asn Thr Thr Asn Ile Asp Gly Phe Arg Leu Asp Ala Val Lys His 
            260                 265                 270         


Ile Lys Phe Ser Phe Phe Pro Asp Trp Leu Ser Asp Val Arg Ser Gln 
        275                 280                 285             


Thr Gly Lys Pro Leu Phe Thr Val Gly Glu Tyr Trp Ser Tyr Asp Ile 
    290                 295                 300                 


Asn Lys Leu His Asn Tyr Ile Met Lys Thr Asn Gly Thr Met Ser Leu 
305                 310                 315                 320 


Phe Asp Ala Pro Leu His Asn Lys Phe Tyr Thr Ala Ser Lys Ser Gly 
                325                 330                 335     


Gly Thr Phe Asp Met Arg Thr Leu Met Thr Asn Thr Leu Met Lys Asp 
            340                 345                 350         


Gln Pro Thr Leu Ala Val Thr Phe Val Asp Asn His Asp Thr Glu Pro 
        355                 360                 365             


Gly Gln Ala Leu Gln Ser Trp Val Asp Pro Trp Phe Lys Pro Leu Ala 
    370                 375                 380                 


Tyr Ala Phe Ile Leu Thr Arg Gln Glu Gly Tyr Pro Cys Val Phe Tyr 
385                 390                 395                 400 


Gly Asp Tyr Tyr Gly Ile Pro Gln Tyr Asn Ile Pro Ser Leu Lys Ser 
                405                 410                 415     


Lys Ile Asp Pro Leu Leu Ile Ala Arg Arg Asp Tyr Ala Tyr Gly Thr 
            420                 425                 430         


Gln His Asp Tyr Leu Asp His Ser Asp Ile Ile Gly Trp Thr Arg Glu 
        435                 440                 445             


Gly Val Thr Glu Lys Pro Gly Ser Gly Leu Ala Ala Leu Ile Thr Asp 
    450                 455                 460                 


Gly Pro Gly Gly Ser Lys Trp Met Tyr Val Gly Lys Gln His Ala Gly 
465                 470                 475                 480 


Lys Val Phe Tyr Asp Leu Thr Gly Asn Arg Ser Asp Thr Val Thr Ile 
                485                 490                 495     


Asn Ser Asp Gly Trp Gly Glu Phe Lys Val Asn Gly Gly Ser Val Ser 
            500                 505                 510         


Val Trp Val Pro Arg Lys Thr Thr Val Ser Thr Ile Ala Trp Ser Ile 
        515                 520                 525             


Thr Thr Arg Pro Trp Thr Asp Glu Phe Val Arg Trp Thr Glu Pro Arg 
    530                 535                 540                 


Leu Val Ala Trp Pro 
545                 


<210>  3
<211>  436
<212>  PRT
<213>  artificial

<220>
<223>  amylase backbone

<400>  3

Met Ala Lys Tyr Ser Glu Leu Glu Glu Gly Gly Val Ile Met Gln Ala 
1               5                   10                  15      


Phe Tyr Trp Asp Val Pro Gly Gly Gly Ile Trp Trp Asp Thr Ile Arg 
            20                  25                  30          


Ser Lys Ile Pro Glu Trp Tyr Glu Ala Gly Ile Ser Ala Ile Trp Ile 
        35                  40                  45              


Pro Pro Ala Ser Lys Gly Met Ser Gly Gly Tyr Ser Met Gly Tyr Asp 
    50                  55                  60                  


Pro Tyr Asp Phe Phe Asp Leu Gly Glu Tyr Asn Gln Lys Gly Thr Ile 
65                  70                  75                  80  


Glu Thr Arg Phe Gly Ser Lys Gln Glu Leu Ile Asn Met Ile Asn Thr 
                85                  90                  95      


Ala His Ala Tyr Gly Ile Lys Val Ile Ala Asp Ile Val Ile Asn His 
            100                 105                 110         


Arg Ala Gly Gly Asp Leu Glu Trp Asn Pro Phe Val Gly Asp Tyr Thr 
        115                 120                 125             


Trp Thr Asp Phe Ser Lys Val Ala Ser Gly Lys Tyr Thr Ala Asn Tyr 
    130                 135                 140                 


Leu Asp Phe His Pro Asn Glu Val Lys Cys Cys Asp Glu Gly Thr Phe 
145                 150                 155                 160 


Gly Gly Phe Pro Asp Ile Ala His Glu Lys Ser Trp Asp Gln His Trp 
                165                 170                 175     


Leu Trp Ala Ser Asp Glu Ser Tyr Ala Ala Tyr Leu Arg Ser Ile Gly 
            180                 185                 190         


Val Asp Ala Trp Arg Phe Asp Tyr Val Lys Gly Tyr Gly Ala Trp Val 
        195                 200                 205             


Val Lys Asp Trp Leu Asn Trp Trp Gly Gly Trp Ala Val Gly Glu Tyr 
    210                 215                 220                 


Trp Asp Thr Asn Val Asp Ala Leu Leu Asn Trp Ala Tyr Ser Ser Gly 
225                 230                 235                 240 


Ala Lys Val Phe Asp Phe Pro Leu Tyr Tyr Lys Met Asp Glu Ala Phe 
                245                 250                 255     


Asp Asn Lys Asn Ile Pro Ala Leu Val Ser Ala Leu Gln Asn Gly Gln 
            260                 265                 270         


Thr Val Val Ser Arg Asp Pro Phe Lys Ala Val Thr Phe Val Ala Asn 
        275                 280                 285             


His Asp Thr Asp Ile Ile Trp Asn Lys Tyr Leu Ala Tyr Ala Phe Ile 
    290                 295                 300                 


Leu Thr Tyr Glu Gly Gln Pro Val Ile Phe Tyr Arg Asp Tyr Glu Glu 
305                 310                 315                 320 


Trp Leu Asn Lys Asp Arg Leu Asn Asn Leu Ile Trp Ile His Asp His 
                325                 330                 335     


Leu Ala Gly Gly Ser Thr Ser Ile Val Tyr Tyr Asp Ser Asp Glu Met 
            340                 345                 350         


Ile Phe Val Arg Asn Gly Tyr Gly Ser Lys Pro Gly Leu Ile Thr Tyr 
        355                 360                 365             


Ile Asn Leu Gly Ser Ser Lys Val Gly Arg Trp Val Tyr Val Pro Lys 
    370                 375                 380                 


Phe Ala Gly Ala Cys Ile His Glu Tyr Thr Gly Asn Leu Gly Gly Trp 
385                 390                 395                 400 


Val Asp Lys Tyr Val Tyr Ser Ser Gly Trp Val Tyr Leu Glu Ala Pro 
                405                 410                 415     


Ala Tyr Asp Pro Ala Asn Gly Gln Tyr Gly Tyr Ser Val Trp Ser Tyr 
            420                 425                 430         


Cys Gly Val Gly 
        435     


<210>  4
<211>  436
<212>  PRT
<213>  artificial

<220>
<223>  amylase backbone

<400>  4

Met Ala Lys Tyr Leu Glu Leu Glu Glu Gly Gly Leu Ile Met Gln Ala 
1               5                   10                  15      


Phe Tyr Trp Asp Val Pro Met Gly Gly Ile Trp Trp Asp Thr Ile Ala 
            20                  25                  30          


Gln Lys Ile Pro Asp Trp Ala Ser Ala Gly Ile Ser Ala Ile Trp Ile 
        35                  40                  45              


Pro Pro Ala Ser Lys Gly Met Ser Gly Gly Tyr Ser Met Gly Tyr Asp 
    50                  55                  60                  


Pro Tyr Asp Tyr Phe Asp Leu Gly Glu Tyr Tyr Gln Lys Gly Thr Val 
65                  70                  75                  80  


Glu Thr Arg Phe Gly Ser Lys Gln Glu Leu Ile Asn Met Ile Asn Thr 
                85                  90                  95      


Ala His Ala Tyr Gly Met Lys Val Ile Ala Asp Ile Val Ile Asn His 
            100                 105                 110         


Arg Ala Gly Gly Asp Leu Glu Trp Asn Pro Phe Val Asn Asp Tyr Thr 
        115                 120                 125             


Trp Thr Asp Phe Ser Lys Val Ala Ser Gly Lys Tyr Thr Ala Asn Tyr 
    130                 135                 140                 


Leu Asp Phe His Pro Asn Glu Leu His Ala Gly Asp Ser Gly Thr Phe 
145                 150                 155                 160 


Gly Gly Tyr Pro Asp Ile Cys His Asp Lys Ser Trp Asp Gln Tyr Trp 
                165                 170                 175     


Leu Trp Ala Ser Gln Glu Ser Tyr Ala Ala Tyr Leu Arg Ser Ile Gly 
            180                 185                 190         


Ile Asp Ala Trp Arg Phe Asp Tyr Val Lys Gly Tyr Ala Pro Trp Val 
        195                 200                 205             


Val Lys Asp Trp Leu Asn Trp Trp Gly Gly Trp Ala Val Gly Glu Tyr 
    210                 215                 220                 


Trp Asp Thr Asn Val Asp Ala Val Leu Asn Trp Ala Tyr Ser Ser Gly 
225                 230                 235                 240 


Ala Lys Val Phe Asp Phe Ala Leu Tyr Tyr Lys Met Asp Glu Ala Phe 
                245                 250                 255     


Asp Asn Asn Asn Ile Pro Ala Leu Val Asp Ala Leu Arg Tyr Gly Gln 
            260                 265                 270         


Thr Val Val Ser Arg Asp Pro Phe Lys Ala Val Thr Phe Val Ala Asn 
        275                 280                 285             


His Asp Thr Asp Ile Ile Trp Asn Lys Tyr Pro Ala Tyr Ala Phe Ile 
    290                 295                 300                 


Leu Thr Tyr Glu Gly Gln Pro Thr Ile Phe Tyr Arg Asp Tyr Glu Glu 
305                 310                 315                 320 


Trp Leu Asn Lys Asp Lys Leu Lys Asn Leu Ile Trp Ile His Asp Asn 
                325                 330                 335     


Leu Ala Gly Gly Ser Thr Asp Ile Val Tyr Tyr Asp Asn Asp Glu Leu 
            340                 345                 350         


Ile Phe Val Arg Asn Gly Tyr Gly Ser Lys Pro Gly Leu Ile Thr Tyr 
        355                 360                 365             


Ile Asn Leu Ala Ser Ser Lys Ala Gly Arg Trp Val Tyr Val Pro Lys 
    370                 375                 380                 


Phe Ala Gly Ser Cys Ile His Glu Tyr Thr Gly Asn Leu Gly Gly Trp 
385                 390                 395                 400 


Val Asp Lys Trp Val Asp Ser Ser Gly Trp Val Tyr Leu Glu Ala Pro 
                405                 410                 415     


Ala His Asp Pro Ala Asn Gly Gln Tyr Gly Tyr Ser Val Trp Ser Tyr 
            420                 425                 430         


Cys Gly Val Gly 
        435     


<210>  5
<211>  436
<212>  PRT
<213>  artificial

<220>
<223>  amylase backbone

<400>  5

Met Ala Lys Tyr Leu Glu Leu Glu Glu Gly Gly Val Ile Met Gln Ala 
1               5                   10                  15      


Phe Tyr Trp Asp Val Pro Ser Gly Gly Ile Trp Trp Asp Thr Ile Arg 
            20                  25                  30          


Gln Lys Ile Pro Glu Trp Tyr Asp Ala Gly Ile Ser Ala Ile Trp Ile 
        35                  40                  45              


Pro Pro Ala Ser Lys Gly Met Gly Gly Ala Tyr Ser Met Gly Tyr Asp 
    50                  55                  60                  


Pro Tyr Asp Phe Phe Asp Leu Gly Glu Tyr Asp Gln Lys Gly Thr Val 
65                  70                  75                  80  


Glu Thr Arg Phe Gly Ser Lys Gln Glu Leu Val Asn Met Ile Asn Thr 
                85                  90                  95      


Ala His Ala Tyr Gly Ile Lys Val Ile Ala Asp Ile Val Ile Asn His 
            100                 105                 110         


Arg Ala Gly Gly Asp Leu Glu Trp Asn Pro Phe Val Asn Asp Tyr Thr 
        115                 120                 125             


Trp Thr Asp Phe Ser Lys Val Ala Ser Gly Lys Tyr Thr Ala Asn Tyr 
    130                 135                 140                 


Leu Asp Phe His Pro Asn Glu Val Lys Cys Cys Asp Glu Gly Thr Phe 
145                 150                 155                 160 


Gly Gly Phe Pro Asp Ile Ala His Glu Lys Ser Trp Asp Gln Tyr Trp 
                165                 170                 175     


Leu Trp Ala Ser Asn Glu Ser Tyr Ala Ala Tyr Leu Arg Ser Ile Gly 
            180                 185                 190         


Val Asp Ala Trp Arg Phe Asp Tyr Val Lys Gly Tyr Gly Ala Trp Val 
        195                 200                 205             


Val Lys Asp Trp Leu Asp Trp Trp Gly Gly Trp Ala Val Gly Glu Tyr 
    210                 215                 220                 


Trp Asp Thr Asn Val Asp Ala Leu Leu Asn Trp Ala Tyr Ser Ser Asp 
225                 230                 235                 240 


Ala Lys Val Phe Asp Phe Pro Leu Tyr Tyr Lys Met Asp Ala Ala Phe 
                245                 250                 255     


Asp Asn Lys Asn Ile Pro Ala Leu Val Glu Ala Leu Lys Asn Gly Gly 
            260                 265                 270         


Thr Val Val Ser Arg Asp Pro Phe Lys Ala Val Thr Phe Val Ala Asn 
        275                 280                 285             


His Asp Thr Asp Ile Ile Trp Asn Lys Tyr Pro Ala Tyr Ala Phe Ile 
    290                 295                 300                 


Leu Thr Tyr Glu Gly Gln Pro Thr Ile Phe Tyr Arg Asp Tyr Glu Glu 
305                 310                 315                 320 


Trp Leu Asn Lys Asp Arg Leu Lys Asn Leu Ile Trp Ile His Asp His 
                325                 330                 335     


Leu Ala Gly Gly Ser Thr Asp Ile Val Tyr Tyr Asp Asn Asp Glu Leu 
            340                 345                 350         


Ile Phe Val Arg Asn Gly Tyr Gly Asp Lys Pro Gly Leu Ile Thr Tyr 
        355                 360                 365             


Ile Asn Leu Gly Ser Ser Lys Ala Gly Arg Trp Val Tyr Val Pro Lys 
    370                 375                 380                 


Phe Ala Gly Ala Cys Ile His Glu Tyr Thr Gly Asn Leu Gly Gly Trp 
385                 390                 395                 400 


Val Asp Lys Trp Val Asp Ser Ser Gly Trp Val Tyr Leu Glu Ala Pro 
                405                 410                 415     


Ala His Asp Pro Ala Asn Gly Tyr Tyr Gly Tyr Ser Val Trp Ser Tyr 
            420                 425                 430         


Cys Gly Val Gly 
        435     


<210>  6
<211>  436
<212>  PRT
<213>  amylase backbone

<400>  6

Met Ala Lys Tyr Ser Glu Leu Glu Lys Gly Gly Val Ile Met Gln Ala 
1               5                   10                  15      


Phe Tyr Trp Asp Val Pro Glu Gly Gly Ile Trp Trp Asp Thr Ile Arg 
            20                  25                  30          


Gln Lys Ile Pro Glu Trp Tyr Asp Ala Gly Ile Ser Ala Ile Trp Ile 
        35                  40                  45              


Pro Pro Ala Ser Lys Gly Met Gly Gly Ala Tyr Ser Met Gly Tyr Asp 
    50                  55                  60                  


Pro Tyr Asp Phe Phe Asp Leu Gly Glu Tyr Asp Gln Lys Gly Thr Val 
65                  70                  75                  80  


Glu Thr Arg Phe Gly Ser Lys Gln Glu Leu Val Asn Met Ile Asn Thr 
                85                  90                  95      


Ala His Ala Tyr Gly Met Lys Val Ile Ala Asp Ile Val Ile Asn His 
            100                 105                 110         


Arg Ala Gly Gly Asp Leu Glu Trp Asn Pro Phe Val Asn Asp Tyr Thr 
        115                 120                 125             


Trp Thr Asp Phe Ser Lys Val Ala Ser Gly Lys Tyr Thr Ala Asn Tyr 
    130                 135                 140                 


Leu Asp Phe His Pro Asn Glu Leu His Ala Gly Asp Ser Gly Thr Phe 
145                 150                 155                 160 


Gly Gly Tyr Pro Asp Ile Cys His Asp Lys Ser Trp Asp Gln Tyr Trp 
                165                 170                 175     


Leu Trp Ala Ser Gln Glu Ser Tyr Ala Ala Tyr Leu Arg Ser Ile Gly 
            180                 185                 190         


Ile Asp Ala Trp Arg Phe Asp Tyr Val Lys Gly Tyr Ala Pro Trp Val 
        195                 200                 205             


Val Lys Asp Trp Leu Asn Trp Trp Gly Gly Trp Ala Val Gly Glu Tyr 
    210                 215                 220                 


Trp Asp Thr Asn Val Asp Ala Val Leu Asn Trp Ala Tyr Ser Ser Gly 
225                 230                 235                 240 


Ala Lys Val Phe Asp Phe Ala Leu Tyr Tyr Lys Met Asp Glu Ala Phe 
                245                 250                 255     


Asp Asn Lys Asn Ile Pro Ala Leu Val Ser Ala Leu Gln Asn Gly Gln 
            260                 265                 270         


Thr Val Val Ser Arg Asp Pro Phe Lys Ala Val Thr Phe Val Ala Asn 
        275                 280                 285             


His Asp Thr Asp Ile Ile Trp Asn Lys Tyr Pro Ala Tyr Ala Phe Ile 
    290                 295                 300                 


Leu Thr Tyr Glu Gly Gln Pro Thr Ile Phe Tyr Arg Asp Tyr Glu Glu 
305                 310                 315                 320 


Trp Leu Asn Lys Asp Lys Leu Lys Asn Leu Ile Trp Ile His Glu Asn 
                325                 330                 335     


Leu Ala Gly Gly Ser Thr Asp Ile Val Tyr Tyr Asp Asn Asp Glu Leu 
            340                 345                 350         


Ile Phe Val Arg Asn Gly Tyr Gly Asp Lys Pro Gly Leu Ile Thr Tyr 
        355                 360                 365             


Ile Asn Leu Gly Ser Ser Lys Ala Gly Arg Trp Val Tyr Val Pro Lys 
    370                 375                 380                 


Phe Ala Gly Ala Cys Ile His Glu Tyr Thr Gly Asn Leu Gly Gly Trp 
385                 390                 395                 400 


Val Asp Lys Tyr Val Tyr Ser Ser Gly Trp Val Tyr Leu Glu Ala Pro 
                405                 410                 415     


Ala Tyr Asp Pro Ala Asn Gly Gln Tyr Gly Tyr Ser Val Trp Ser Tyr 
            420                 425                 430         


Cys Gly Val Gly 
        435     


<210>  7
<211>  436
<212>  PRT
<213>  amylase backbone

<400>  7

Met Ala Lys Tyr Leu Glu Leu Glu Glu Gly Gly Val Ile Met Gln Ala 
1               5                   10                  15      


Phe Tyr Trp Asp Val Pro Gly Gly Gly Ile Trp Trp Asp His Ile Arg 
            20                  25                  30          


Ser Lys Ile Pro Glu Trp Tyr Glu Ala Gly Ile Ser Ala Ile Trp Leu 
        35                  40                  45              


Pro Pro Pro Ser Lys Gly Met Ser Gly Gly Tyr Ser Met Gly Tyr Asp 
    50                  55                  60                  


Pro Tyr Asp Tyr Phe Asp Leu Gly Glu Tyr Tyr Gln Lys Gly Thr Val 
65                  70                  75                  80  


Glu Thr Arg Phe Gly Ser Lys Glu Glu Leu Val Arg Leu Ile Gln Thr 
                85                  90                  95      


Ala His Ala Tyr Gly Ile Lys Val Ile Ala Asp Val Val Ile Asn His 
            100                 105                 110         


Arg Ala Gly Gly Asp Leu Glu Trp Asn Pro Phe Val Gly Asp Tyr Thr 
        115                 120                 125             


Trp Thr Asp Phe Ser Lys Val Ala Ser Gly Lys Tyr Thr Ala Asn Tyr 
    130                 135                 140                 


Leu Asp Phe His Pro Asn Glu Leu His Cys Cys Asp Glu Gly Thr Phe 
145                 150                 155                 160 


Gly Gly Phe Pro Asp Ile Cys His His Lys Glu Trp Asp Gln Tyr Trp 
                165                 170                 175     


Leu Trp Lys Ser Asn Glu Ser Tyr Ala Ala Tyr Leu Arg Ser Ile Gly 
            180                 185                 190         


Phe Asp Gly Trp Arg Phe Asp Tyr Val Lys Gly Tyr Gly Ala Trp Val 
        195                 200                 205             


Val Arg Asp Trp Leu Asn Trp Trp Gly Gly Trp Ala Val Gly Glu Tyr 
    210                 215                 220                 


Trp Asp Thr Asn Val Asp Ala Leu Leu Ser Trp Ala Tyr Glu Ser Gly 
225                 230                 235                 240 


Ala Lys Val Phe Asp Phe Pro Leu Tyr Tyr Lys Met Asp Glu Ala Phe 
                245                 250                 255     


Asp Asn Asn Asn Ile Pro Ala Leu Val Tyr Ala Leu Gln Asn Gly Gln 
            260                 265                 270         


Thr Val Val Ser Arg Asp Pro Phe Lys Ala Val Thr Phe Val Ala Asn 
        275                 280                 285             


His Asp Thr Asp Ile Ile Trp Asn Lys Tyr Pro Ala Tyr Ala Phe Ile 
    290                 295                 300                 


Leu Thr Tyr Glu Gly Gln Pro Val Ile Phe Tyr Arg Asp Phe Glu Glu 
305                 310                 315                 320 


Trp Leu Asn Lys Asp Lys Leu Ile Asn Leu Ile Trp Ile His Asp His 
                325                 330                 335     


Leu Ala Gly Gly Ser Thr Thr Ile Val Tyr Tyr Asp Asn Asp Glu Leu 
            340                 345                 350         


Ile Phe Val Arg Asn Gly Asp Ser Arg Arg Pro Gly Leu Ile Thr Tyr 
        355                 360                 365             


Ile Asn Leu Ser Pro Asn Trp Val Gly Arg Trp Val Tyr Val Pro Lys 
    370                 375                 380                 


Phe Ala Gly Ala Cys Ile His Glu Tyr Thr Gly Asn Leu Gly Gly Trp 
385                 390                 395                 400 


Val Asp Lys Arg Val Asp Ser Ser Gly Trp Val Tyr Leu Glu Ala Pro 
                405                 410                 415     


Pro His Asp Pro Ala Asn Gly Tyr Tyr Gly Tyr Ser Val Trp Ser Tyr 
            420                 425                 430         


Cys Gly Val Gly 
        435     


