                         SEQUENCE LISTING

<110>  New England Biolabs, Inc.
 
<120>  Compositions and Methods for Improved In Vitro Assembly of 
       Polynucleotides

<130>  NEB-411-442-449-PCT

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  510
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  1

Met Pro Tyr Asp His Asn Ala Glu Ala Asp Phe Ala Ala Ser Glu Val 
1               5                   10                  15      


Ala Arg Met Leu Val Ala Asp Pro Gly Leu Cys Tyr Asp Ala Ala Ser 
            20                  25                  30          


Leu Pro Ala Ser Ile Ser Ala Ser Ala Ser Tyr Glu Pro Ser Ala Ala 
        35                  40                  45              


Gly Trp Pro Lys Ala Asp Gly Leu Val Ser Val Leu Glu Gly Gly Thr 
    50                  55                  60                  


Ser Thr Gln Arg Ala Ile Ala Leu Glu Tyr Lys Arg Pro Gln Glu Gly 
65                  70                  75                  80  


Ile His Gly Leu Leu Thr Ala Ile Gly Gln Ala His Gly Tyr Leu His 
                85                  90                  95      


Lys Gly Tyr Ser Gly Ala Ala Ile Val Ile Pro Gly Arg Tyr Ser Ser 
            100                 105                 110         


His Pro Thr Pro Ala Glu Tyr Val Arg Asp Val Leu Asn Ala Ile Ser 
        115                 120                 125             


Gly Ser Arg Ala Ile Ala Val Phe Ser Tyr Ser Pro Pro Asp Thr Thr 
    130                 135                 140                 


Ser Pro Thr Pro Phe Ala Gly Arg Ile Gln Cys Val Arg Pro Leu Val 
145                 150                 155                 160 


Phe Asp Ala Gly Arg Val His Leu Arg Pro Ala Asn Gln Gly Pro Lys 
                165                 170                 175     


Thr Gln Trp Val His Met Arg Glu Gly Ser Thr Thr Arg Asp Ala Phe 
            180                 185                 190         


Phe Arg Phe Leu Gln Val Ala Lys Arg Leu Ser Ala Asp Pro Thr Ala 
        195                 200                 205             


Pro Arg Pro Thr Leu Arg Ser Glu Leu Val Ala Ala Ile Gly Arg Leu 
    210                 215                 220                 


Ala Pro Gly Arg Asp Pro Ile Glu Tyr Ile Thr Asn Thr Ala Asp Asn 
225                 230                 235                 240 


Lys Phe Leu Thr Lys Val Trp Gln Phe Phe Trp Leu Glu Trp Leu Ala 
                245                 250                 255     


Thr Pro Ala Val Leu Thr Pro Trp Lys Leu Glu Ala Gly Val Tyr Ser 
            260                 265                 270         


Ala Pro Gly Ala Arg Thr Arg Ile Leu Arg Glu Asp Gly Thr Asp Phe 
        275                 280                 285             


Ser Gln Leu Trp Glu Gly Arg Val Asn Ser Leu Lys Glu Thr Ile Ala 
    290                 295                 300                 


Gly Met Leu Asn Arg Gly Glu Ile Ser Glu Ala Gln Gly Trp Glu Ala 
305                 310                 315                 320 


Phe Val Gly Gly Ile Ser Ala Thr Gly Gly Gly Gln Asp Lys Gln Gly 
                325                 330                 335     


Val Arg Ala Arg Ala His Ser Tyr Arg Glu Asp Ile Asp Ser Ala Leu 
            340                 345                 350         


Ala Gln Leu Arg Trp Ile Glu Asp Asp Gly Leu Pro Thr Asp Gln Gly 
        355                 360                 365             


Tyr Arg Phe Met Thr Ile Cys Glu Arg Tyr Gly Gly Ala Asn Ser Arg 
    370                 375                 380                 


Ala Ala Ile Asp Tyr Met Gly Ala Thr Leu Ile Gln Thr Gly Arg Tyr 
385                 390                 395                 400 


Ala Ser Phe Leu His Tyr Ile Asn Arg Leu Ser Glu Arg Lys Phe Ala 
                405                 410                 415     


Glu Asn Pro Leu Ala Tyr Thr Lys Pro Gly Pro Gly Gly Met Pro Val 
            420                 425                 430         


Phe Thr Glu Glu Ser Tyr Trp Glu Tyr Leu Gln Asp Leu Glu Thr Lys 
        435                 440                 445             


Leu Thr Asp Glu Leu Arg Val Met Arg Lys Val Ser Gly Arg Ala Arg 
    450                 455                 460                 


Pro Arg Val Arg Thr Thr Phe Gln Val Glu Leu Thr Leu Leu Arg Asn 
465                 470                 475                 480 


Tyr Gly Phe Val Ser Ser Thr Arg His Arg Leu Gly Val Gly Ile Pro 
                485                 490                 495     


Ile Asp Trp Glu Gln Val Val Gln Ala Leu Asn Val Asp Leu 
            500                 505                 510 


<210>  2
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (8)..(15)
<223>  n is a, c, g, or t

<400>  2
cacctgcnnn nnnnn                                                        15


<210>  3
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (8)..(15)
<223>  n is a, c, g, or t

<400>  3
gtggacgnnn nnnnn                                                        15


<210>  4
<211>  1620
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  4
accatcatta cccattcact gcccctcctt ttacaacctc ctcactccca aaaccctggc       60

cttacccaac ttaatcccct tccaccacat ccccctttcc ccacctcccc taatagccaa      120

cacgccccca cccatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt      180

gcctcctttc ccccaccaca agccgtcccg caaagctggc tggagtgcga tcttcctcac      240

ccccatactc tcctcgtccc ctcaaactgc cacatccacg gttaccatgc gcccatctac      300

accaacctaa cctatcccat taccctcaat ccgccgtttc ttcccaccca caatccgacc      360

cgttcttact cgctcacatt taatgttcat caaacctccc tacaccaacc ccacacccca      420

attatttttc atcccgttaa ctccgcgttt catctctcct gcaacccccc ctccctccct      480

tacccccacc acagtcgttt gccgtctgaa tttgacctca gcgcattttt acgcccccca      540

caaaaccccc tcgccctcat cgtcctccct tgcagtgacc ccagttatct cgaagatcac      600

gatatctccc ccatcacccc cattttccct cacctctcct tcctccataa acccactaca      660

caaatcagcg atttccatgt tgccactccc tttaatgatc atttcagccc cgctgtactg      720

caggctgaag ttcagatgtc ccgcgacttc cctcactacc tacgcctaac actttcttta      780

tggcaccctc aaacccacct ccccacccgc accccccctt tccccgctca aattatccat      840

caccctcctc cttatcccca tcccctcaca ctacctctca acctccaaaa ccccaaactc      900

tccacccccc aaatccccaa tctctatcct cccctccttg aactccacac cccccacccc      960

accctgattc aaccacaacc ctcccatctt ccctttcccc cacctcccca ttgaaaatcc     1020

tctcctcctc ctgaacccca accccttcct cattccaccc cttaaccctc accaccatca     1080

tcctctccat cctcacgtca tccatcacca caccatcctc caccatatcc tcctcatcaa     1140

ccacaacaac tttaaccccc tcccctgttc ccattatccc aaccatcccc tctgctacac     1200

cctctccgac cgctaccccc tctatctcct ccatgaaccc aatattcaaa cccaccgcat     1260

cctcccaatc aatcctctca cccatcatcc ccgctcccta ccccccatca cccaacccct     1320

aaccccaatc ctccaccccc atcctaatca ccccactctc atcatctcct ccctcgccaa     1380

tcaatcaccc caccccccta atcaccaccc gctctatccc tgcatcaaat ctctccatcc     1440

ttcccccccc ctgcactatc aacccccccc accccacacc acccccaccc atattatttc     1500

ccccatctac ccccccctcc atcaacacca ccccttcccc cctctcccca aatcctccat     1560

caaaaaatcc ctttccctac ctccacacac cccccccctc atcctttccc aataccccca     1620


