<110>    Ewha University - Industry Collaboration Foundation

<120>    Nucleic Acid Molecules with Increased Gene Silencing Activity and
         Uses Thereof

<130>    OPP20211484KR

<150>    KR 10-2020-0179036
<151>    2020-12-18

<160>    78

<170>    koPatentIn 3.0

<210>    1
<211>    25
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    sense_HRRT_non_modi


<400>    1
ccagacuuug uuggauuuga agugc                                               25


<210>    2
<211>    27
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    antisense_HRRT_non_modi


<400>    2
gcacuucaaa uccaacaaag ucuggca                                             27


<210>    3
<211>    27
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    antisense_HRRT_18_Ome


<220>
<221>    modified_base
<222>    (18)
<223>    2'-Ome


<400>    3
gcacuucaaa uccaacaaag ucuggca                                             27


<210>    4
<211>    27
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    antisense_HRRT_19_Ome


<220>
<221>    modified_base
<222>    (19)
<223>    2'-Ome


<400>    4
gcacuucaaa uccaacaaag ucuggca                                             27


<210>    5
<211>    27
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    antisense_HRRT_18_19_Ome


<220>
<221>    modified_base
<222>    (18)..(19)
<223>    2'-Ome


<400>    5
gcacuucaaa uccaacaaag ucuggca                                             27


<210>    6
<211>    25
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    sense_GFP_non_modi


<400>    6
gcaagcugac ccugaaguua ucacc                                               25


<210>    7
<211>    27
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    antisense_GFP_non_modi


<400>    7
ggugauaacu ucagggucag cuugcca                                             27


<210>    8
<211>    27
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    antisense_GFP_18_Ome


<220>
<221>    modified_base
<222>    (18)
<223>    2'-Ome


<400>    8
ggugauaacu ucagggucag cuugcca                                             27


<210>    9
<211>    27
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    antisense_GFP_19_Ome


<220>
<221>    modified_base
<222>    (19)
<223>    2'-Ome


<400>    9
ggugauaacu ucagggucag cuugcca                                             27


<210>    10
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    HRRT_Forward_primer


<400>    10
gactttgctt tccttggtca g                                                   21


<210>    11
<211>    24
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    HRRT_Reverse_primer


<400>    11
ggcttatatc caacacttcg tggg                                                24


<210>    12
<211>    18
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    GAPDH_Forward_primer


<400>    12
ggatttggtc gtattggg                                                       18


<210>    13
<211>    19
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    GAPDH_Reverse_primer


<400>    13
ggaagatggt gatgggatt                                                      19


<210>    14
<211>    25
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    sense_HRRT_19-21bp_modi


<220>
<221>    modified_base
<222>    (21)..(23)
<223>    2'-Ome


<400>    14
ccagacuuug uuggauuuga agugc                                               25


<210>    15
<211>    27
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    antisense_HRRT_19-21bp_modi


<220>
<221>    modified_base
<222>    (5)..(7)
<223>    2'-Ome


<400>    15
gcacuucaaa uccaacaaag ucuggca                                             27


<210>    16
<211>    25
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    sense_HRRT_~15bp_modi


<220>
<221>    modified_base
<222>    (1)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (2)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (6)..(9)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (11)..(12)
<223>    2'-Ome


<400>    16
ccagacuuug uuggauuuga agugc                                               25


<210>    17
<211>    27
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    antisense_HRRT_~15bp_modi


<220>
<221>    modified_base
<222>    (13)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (16)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (26)
<223>    2'-Ome


<400>    17
gcacuucaaa uccaacaaag ucuggca                                             27


<210>    18
<211>    25
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    sense_HRRT_~15bp+19-21bp_modi


<220>
<221>    modified_base
<222>    (1)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (2)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (6)..(9)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (11)..(12)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (16)..(18)
<223>    2'-Ome


<400>    18
ccagacuuug uuggauuuga agugc                                               25


<210>    19
<211>    27
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    antisense_HRRT_~15bp+19-21bp_modi


<220>
<221>    modified_base
<222>    (7)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (13)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (16)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (26)
<223>    2'-Ome


<400>    19
gcacuucaaa uccaacaaag ucuggca                                             27


<210>    20
<211>    25
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    sense_HRRT_C/U_modi


<220>
<221>    modified_base
<222>    (1)..(2)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (6)..(9)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (11)..(12)
<223>    2'-Ome


<400>    20
ccagacuuug uuggauuuga agugc                                               25


<210>    21
<211>    27
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    antisense_HRRT_C/U_modi


<220>
<221>    modified_base
<222>    (11)..(13)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (16)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (21)..(23)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (26)
<223>    2'-Ome


<400>    21
gcacuucaaa uccaacaaag ucuggca                                             27


<210>    22
<211>    25
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    sense_HRRT_Alt_modi


<220>
<221>    modified_base
<222>    (2)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (4)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (6)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (8)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (10)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (12)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (14)
<223>    2'-Ome


<400>    22
ccagacuuug uuggauuuga agugc                                               25


<210>    23
<211>    27
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    antisense_HRRT_Alt_modi


<220>
<221>    modified_base
<222>    (11)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (13)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (15)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (17)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (19)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (21)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (23)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (25)
<223>    2'-Ome


<400>    23
gcacuucaaa uccaacaaag ucuggca                                             27


<210>    24
<211>    25
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    sense_GFP_P(+1)P_9_Ome


<220>
<221>    modified_base
<222>    (1)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (4)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (5)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (7)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (9)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (14)
<223>    2'-Ome


<400>    24
gcaagcugac ccugaaguua ucacc                                               25


<210>    25
<211>    27
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    antisense_GFP_P(+1)P


<220>
<221>    modified_base
<222>    (13)..(16)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (18)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (20)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (23)..(24)
<223>    2'-Ome


<400>    25
ggugauaacu ucagggucag cuugcca                                             27


<210>    26
<211>    25
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    sense_GFP_P(+1)P


<220>
<221>    modified_base
<222>    (1)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (4)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (5)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (7)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (14)
<223>    2'-Ome


<400>    26
gcaagcugac ccugaaguua ucacc                                               25


<210>    27
<211>    25
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    sense_HRRT_P(+1)P


<220>
<221>    modified_base
<222>    (1)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (4)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (5)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (7)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (14)
<223>    2'-Ome


<400>    27
ccagacuuug uuggauuuga agugc                                               25


<210>    28
<211>    27
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    antisense_HRRT_P(+1)P


<220>
<221>    modified_base
<222>    (13)..(16)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (18)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (20)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (23)..(24)
<223>    2'-Ome


<400>    28
gcacuucaaa uccaacaaag ucuggca                                             27


<210>    29
<211>    21
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    sense_HRRT_siRNA


<400>    29
ccagacuuug uuggauuugt t                                                   21


<210>    30
<211>    21
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    antisense_HRRT_siRNA


<400>    30
caaauccaac aaagucuggt t                                                   21


<210>    31
<211>    52
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    strand1_Y-RNA_HRRT/FVII_P(+1)P


<220>
<221>    modified_base
<222>    (1)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (4)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (5)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (7)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (14)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (38)..(41)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (43)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (45)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (48)..(49)
<223>    2'-Ome


<400>    31
ccagacuuug uuggauuuga agugcgguga uguaagacuu gagaugaucc ca                 52


<210>    32
<211>    52
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    strand2_Y-RNA_FVII/mTTR_P(+1)P


<220>
<221>    modified_base
<222>    (1)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (4)..(5)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (7)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (14)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (38)..(41)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (43)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (45)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (48)..(49)
<223>    2'-Ome


<400>    32
ggaucaucuc aagucuuaca ucacccgucu auuauagagc aagaacacug ca                 52


<210>    33
<211>    52
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    strand3_Y-RNA_mTTR/HRRT_P(+1)P


<220>
<221>    modified_base
<222>    (1)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (4)..(5)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (7)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (14)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (38)..(41)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (43)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (45)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (48)..(49)
<223>    2'-Ome


<400>    33
caguguucuu gcucuauaau agacggcacu ucaaauccaa caaagucugg ca                 52


<210>    34
<211>    25
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    sense_mTTR_P(+1)P


<220>
<221>    modified_base
<222>    (1)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (4)..(5)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (7)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (14)
<223>    2'-Ome


<400>    34
caguguucuu gcucuauaau agacg                                               25


<210>    35
<211>    21
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    sense_mTTR_siRNA


<400>    35
caguguucuu gcucuauaat t                                                   21


<210>    36
<211>    21
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    antisense_mTTR_siRNA


<400>    36
uuauagagca agaacacugt t                                                   21


<210>    37
<211>    21
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    sense_FVII_siRNA


<400>    37
ggaucaucuc aagucuuact t                                                   21


<210>    38
<211>    21
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    antisense_FVII_siRNA


<400>    38
guaagacuug agaugaucct t                                                   21


<210>    39
<211>    25
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    sense_mTTR_non_modi


<400>    39
caguguucuu gcucuauaau agacg                                               25


<210>    40
<211>    27
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    antisense_mTTR_non_modi


<400>    40
cgucuauuau agagcaagaa cacugca                                             27


<210>    41
<211>    25
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    sense_FVII_non_modi


<400>    41
ggaucaucuc aagucuuaca ucacc                                               25


<210>    42
<211>    27
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    antisense_FVII_non_modi


<400>    42
ggugauguaa gacuugagau gauccca                                             27


<210>    43
<211>    27
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    antisense_mTTR_P(+1)P


<220>
<221>    modified_base
<222>    (13)..(16)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (18)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (20)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (23)..(24)
<223>    2'-Ome


<400>    43
cgucuauuau agagcaagaa cacugca                                             27


<210>    44
<211>    25
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    sense_FVII_P(+1)P


<220>
<221>    modified_base
<222>    (1)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (4)..(5)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (7)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (14)
<223>    2'-Ome


<400>    44
ggaucaucuc aagucuuaca ucacc                                               25


<210>    45
<211>    27
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    antisense_FVII_P(+1)P


<220>
<221>    modified_base
<222>    (13)..(16)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (18)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (20)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (23)..(24)
<223>    2'-Ome


<400>    45
ggugauguaa gacuugagau gauccca                                             27


<210>    46
<211>    52
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    strand1_Y-RNA_HRRT/FVII_non_modi


<400>    46
ccagacuuug uuggauuuga agugcgguga uguaagacuu gagaugaucc ca                 52


<210>    47
<211>    52
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    strand2_Y-RNA_FVII/mTTR_non_modi


<400>    47
ggaucaucuc aagucuuaca ucacccgucu auuauagagc aagaacacug ca                 52


<210>    48
<211>    52
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    strand3_Y-RNA_mTTR/HRRT_non_modi


<400>    48
caguguucuu gcucuauaau agacggcacu ucaaauccaa caaagucugg ca                 52


<210>    49
<211>    25
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    sense_TP53_non_modi


<400>    49
accagggcag cuacgguuua agugc                                               25


<210>    50
<211>    27
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    antisense_TP53_non_modi


<400>    50
gcacuuaaac cguagcugcc cugguca                                             27


<210>    51
<211>    25
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    sense_TP53_P(+1)P


<220>
<221>    modified_base
<222>    (1)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (4)..(5)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (7)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (14)
<223>    2'-Ome


<400>    51
accagggcag cuacgguuua agugc                                               25


<210>    52
<211>    27
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    antisense_TP53_P(+1)P


<220>
<221>    modified_base
<222>    (13)..(16)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (18)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (20)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (23)..(24)
<223>    2'-Ome


<400>    52
gcacuuaaac cguagcugcc cugguca                                             27


<210>    53
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    TP53_Forward_primer


<400>    53
ttccgagagc tgaatgaggc                                                     20


<210>    54
<211>    25
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    TP53_Reverse_primer


<400>    54
gaagtggaga atgtcagtct gagtc                                               25


<210>    55
<211>    25
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    sense_FVII_C/U_modi


<220>
<221>    modified_base
<222>    (4)..(5)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (7)..(10)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (14)..(15)
<223>    2'-Ome


<400>    55
ggaucaucuc aagucuuaca ucacc                                               25


<210>    56
<211>    27
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    antisense_FVII_C/U_modi


<220>
<221>    modified_base
<222>    (13)..(15)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (20)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (23)..(26)
<223>    2'-Ome


<400>    56
ggugauguaa gacuugagau gauccca                                             27


<210>    57
<211>    25
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    sense_mTTR_P(+1)P_(-4)


<220>
<221>    modified_base
<222>    (1)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (5)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (7)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (14)
<223>    2'-Ome


<400>    57
caguguucuu gcucuauaau agacg                                               25


<210>    58
<211>    25
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    sense_mTTR_P(+1)P_(-5)


<220>
<221>    modified_base
<222>    (1)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (4)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (7)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (14)
<223>    2'-Ome


<400>    58
caguguucuu gcucuauaau agacg                                               25


<210>    59
<211>    25
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    sense_mTTR_P(+1)P_(-7)


<220>
<221>    modified_base
<222>    (1)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (4)..(5)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (14)
<223>    2'-Ome


<400>    59
caguguucuu gcucuauaau agacg                                               25


<210>    60
<211>    25
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    sense_mTTR_P(+1)P_(-14)


<220>
<221>    modified_base
<222>    (1)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (4)..(5)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (7)
<223>    2'-Ome


<400>    60
caguguucuu gcucuauaau agacg                                               25


<210>    61
<211>    25
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    sense_HRRT_P(-1)P


<220>
<221>    modified_base
<222>    (4)..(5)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (7)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (14)
<223>    2'-Ome


<400>    61
ccagacuuug uuggauuuga agugc                                               25


<210>    62
<211>    27
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    antisense_HRRT_P(-1)P


<220>
<221>    modified_base
<222>    (13)..(16)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (18)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (20)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (23)..(24)
<223>    2'-Ome


<400>    62
gcacuucaaa uccaacaaag ucuggca                                             27


<210>    63
<211>    25
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    sense_GFP_P(-1)P


<220>
<221>    modified_base
<222>    (4)..(5)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (7)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (14)
<223>    2'-Ome


<400>    63
gcaagcugac ccugaaguua ucacc                                               25


<210>    64
<211>    27
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    antisense_GFP_P(-1)P


<220>
<221>    modified_base
<222>    (13)..(16)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (18)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (20)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (23)..(24)
<223>    2'-Ome


<400>    64
ggugauaacu ucagggucag cuugcca                                             27


<210>    65
<211>    25
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    sense_FVII_P(-1)P


<220>
<221>    modified_base
<222>    (4)..(5)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (7)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (14)
<223>    2'-Ome


<400>    65
ggaucaucuc aagucuuaca ucacc                                               25


<210>    66
<211>    27
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    antisense_FVII_P(-1)P


<220>
<221>    modified_base
<222>    (13)..(16)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (18)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (20)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (23)..(24)
<223>    2'-Ome


<400>    66
ggugauguaa gacuugagau gauccca                                             27


<210>    67
<211>    25
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    sense_mTTR_P(-1)P


<220>
<221>    modified_base
<222>    (4)..(5)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (7)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (14)
<223>    2'-Ome


<400>    67
caguguucuu gcucuauaau agacg                                               25


<210>    68
<211>    27
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    antisense_mTTR_P(-1)P


<220>
<221>    modified_base
<222>    (13)..(16)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (18)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (20)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (23)..(24)
<223>    2'-Ome


<400>    68
cgucuauuau agagcaagaa cacugca                                             27


<210>    69
<211>    52
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    strand1_Y-RNA_HRRT/FVII_P(-1)P


<220>
<221>    modified_base
<222>    (4)..(5)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (7)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (14)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (38)..(41)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (43)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (45)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (48)..(49)
<223>    2'-Ome


<400>    69
ccagacuuug uuggauuuga agugcgguga uguaagacuu gagaugaucc ca                 52


<210>    70
<211>    52
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    strand2_Y-RNA_FVII/mTTR_P(-1)P


<220>
<221>    modified_base
<222>    (4)..(5)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (7)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (14)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (38)..(41)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (43)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (45)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (48)..(49)
<223>    2'-Ome


<400>    70
ggaucaucuc aagucuuaca ucacccgucu auuauagagc aagaacacug ca                 52


<210>    71
<211>    23
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_sense_GFP_non_modi_23nt


<400>    71
gcaagcugac ccugaaguua uca                                                 23


<210>    72
<211>    25
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_antisense_GFP_non_modi_25nt


<400>    72
ugauaacuuc agggucagcu ugcca                                               25


<210>    73
<211>    23
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_sense_GFP_P(+1)P_23nt


<220>
<221>    modified_base
<222>    (1)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (4)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (5)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (7)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (14)
<223>    2'-Ome


<400>    73
gcaagcugac ccugaaguua uca                                                 23


<210>    74
<211>    25
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_antisense_GFP_P(+1)P_25nt


<220>
<221>    modified_base
<222>    (11)..(14)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (16)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (18)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (21)..(22)
<223>    2'-Ome


<400>    74
ugauaacuuc agggucagcu ugcca                                               25


<210>    75
<211>    30
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_sense_GFP_non_modi_30nt


<400>    75
gcaagcugac ccugaaguua ucaccucacc                                          30


<210>    76
<211>    32
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_antisense_GFP_non_modi_32nt


<400>    76
ggugagguga uaacuucagg gucagcuugc ca                                       32


<210>    77
<211>    30
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_sense_GFP_P(+1)P_30nt


<220>
<221>    modified_base
<222>    (1)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (4)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (5)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (7)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (14)
<223>    2'-Ome


<400>    77
gcaagcugac ccugaaguua ucaccucacc                                          30


<210>    78
<211>    32
<212>    RNA
<213>    Artificial Sequence

<220>
<223>    synthetic_antisense_GFP_P(+1)P_32nt


<220>
<221>    modified_base
<222>    (18)..(21)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (23)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (25)
<223>    2'-Ome


<220>
<221>    modified_base
<222>    (28)..(29)
<223>    2'-Ome


<400>    78
ggugagguga uaacuucagg gucagcuugc ca                                       32

