<110>    Korea Institute of Ceramic Engineering and Technology

<120>    IMMUNOPRECIPITATION USING ANTIBODY BINDING PROTEIN-CALSEQUESTRIN
         FUSION PROTEIN

<130>    FP210211PCT

<150>    KR 10-2020-0177994
<151>    2020-12-18

<160>    9

<170>    KoPatentIn 3.0

<210>    1
<211>    362
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CSQ1


<400>    1
Gln Glu Gly Leu Asp Phe Pro Glu Tyr Asp Gly Val Asp Arg Val Ile
  1               5                  10                  15 

Asn Val Asn Ala Lys Asn Tyr Lys Asn Val Phe Lys Lys Tyr Glu Val
             20                  25                  30 

Leu Ala Leu Leu Tyr His Glu Pro Pro Glu Asp Asp Lys Ala Ser Gln
         35                  40                  45 

Arg Gln Phe Glu Met Glu Glu Leu Ile Leu Glu Leu Ala Ala Gln Val
     50                  55                  60 

Leu Glu Asp Lys Gly Val Gly Phe Gly Leu Val Asp Ser Glu Lys Asp
 65                  70                  75                  80 

Ala Ala Val Ala Lys Lys Leu Gly Leu Thr Glu Val Asp Ser Met Tyr
                 85                  90                  95 

Val Phe Lys Gly Asp Glu Val Ile Glu Tyr Asp Gly Glu Phe Ser Ala
            100                 105                 110 

Asp Thr Ile Val Glu Phe Leu Leu Asp Val Leu Glu Asp Pro Val Glu
        115                 120                 125 

Leu Ile Glu Gly Glu Arg Glu Leu Gln Ala Phe Glu Asn Ile Glu Asp
    130                 135                 140 

Glu Ile Lys Leu Ile Gly Tyr Phe Lys Ser Lys Asp Ser Glu His Tyr
145                 150                 155                 160 

Lys Ala Phe Glu Asp Ala Ala Glu Glu Phe His Pro Tyr Ile Pro Phe
                165                 170                 175 

Phe Ala Thr Phe Asp Ser Lys Val Ala Lys Lys Leu Thr Leu Lys Leu
            180                 185                 190 

Asn Glu Ile Asp Phe Tyr Glu Ala Phe Met Glu Glu Pro Val Thr Ile
        195                 200                 205 

Pro Asp Lys Pro Asn Ser Glu Glu Glu Ile Val Asn Phe Val Glu Glu
    210                 215                 220 

His Arg Arg Ser Thr Leu Arg Lys Leu Lys Pro Glu Ser Met Tyr Glu
225                 230                 235                 240 

Thr Trp Glu Asp Asp Met Asp Gly Ile His Ile Val Ala Phe Ala Glu
                245                 250                 255 

Glu Ala Asp Pro Asp Gly Phe Glu Phe Leu Glu Thr Leu Lys Ala Val
            260                 265                 270 

Ala Gln Asp Asn Thr Glu Asn Pro Asp Leu Ser Ile Ile Trp Ile Asp
        275                 280                 285 

Pro Asp Asp Phe Pro Leu Leu Val Pro Tyr Trp Glu Lys Thr Phe Asp
    290                 295                 300 

Ile Asp Leu Ser Ala Pro Gln Ile Gly Val Val Asn Val Thr Asp Ala
305                 310                 315                 320 

Asp Ser Val Trp Met Glu Met Asp Asp Glu Glu Asp Leu Pro Ser Ala
                325                 330                 335 

Glu Glu Leu Glu Asp Trp Leu Glu Asp Val Leu Glu Gly Glu Ile Asn
            340                 345                 350 

Thr Glu Asp Asp Asp Asp Asp Asp Asp Asp
        355                 360        


<210>    2
<211>    364
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CSQ2(Delta)


<400>    2
Glu Glu Gly Leu Asn Phe Pro Thr Tyr Asp Gly Lys Asp Arg Val Val
  1               5                  10                  15 

Ser Leu Ser Glu Lys Asn Phe Lys Gln Val Leu Lys Lys Tyr Asp Leu
             20                  25                  30 

Leu Cys Leu Tyr Tyr His Glu Pro Val Ser Ser Asp Lys Val Thr Pro
         35                  40                  45 

Lys Gln Phe Gln Leu Lys Glu Ile Val Leu Glu Leu Val Ala Gln Val
     50                  55                  60 

Leu Glu His Lys Ala Ile Gly Phe Val Met Val Asp Ala Lys Lys Glu
 65                  70                  75                  80 

Ala Lys Leu Ala Lys Lys Leu Gly Phe Asp Glu Glu Gly Ser Leu Tyr
                 85                  90                  95 

Ile Leu Lys Gly Asp Arg Thr Ile Glu Phe Asp Gly Glu Phe Ala Ala
            100                 105                 110 

Asp Val Leu Val Glu Phe Leu Leu Asp Leu Ile Glu Asp Pro Val Glu
        115                 120                 125 

Ile Ile Ser Ser Lys Leu Glu Val Gln Ala Phe Glu Arg Ile Glu Asp
    130                 135                 140 

Tyr Ile Lys Leu Ile Gly Phe Phe Lys Ser Glu Asp Ser Glu Tyr Tyr
145                 150                 155                 160 

Lys Ala Phe Glu Glu Ala Ala Glu His Phe Gln Pro Tyr Ile Lys Phe
                165                 170                 175 

Phe Ala Thr Phe Asp Lys Gly Val Ala Lys Lys Leu Ser Leu Lys Met
            180                 185                 190 

Asn Glu Val Asp Phe Tyr Glu Pro Phe Met Asp Glu Pro Ile Ala Ile
        195                 200                 205 

Pro Asn Lys Pro Tyr Thr Glu Glu Glu Leu Val Glu Phe Val Lys Glu
    210                 215                 220 

His Gln Arg Pro Thr Leu Arg Arg Leu Arg Pro Glu Glu Met Phe Glu
225                 230                 235                 240 

Thr Trp Glu Asp Asp Leu Asn Gly Ile His Ile Val Ala Phe Ala Glu
                245                 250                 255 

Lys Ser Asp Pro Asp Gly Tyr Glu Phe Leu Glu Ile Leu Lys Gln Val
            260                 265                 270 

Ala Arg Asp Asn Thr Asp Asn Pro Asp Leu Ser Ile Leu Trp Ile Asp
        275                 280                 285 

Pro Asp Asp Phe Pro Leu Leu Val Ala Tyr Trp Glu Lys Thr Phe Lys
    290                 295                 300 

Ile Asp Leu Phe Arg Pro Gln Ile Gly Val Val Asn Val Thr Asp Ala
305                 310                 315                 320 

Asp Ser Val Trp Met Glu Ile Pro Asp Asp Asp Asp Leu Pro Thr Ala
                325                 330                 335 

Glu Glu Leu Glu Asp Trp Ile Glu Asp Val Leu Ser Gly Lys Ile Asn
            340                 345                 350 

Thr Glu Asp Asp Asp Glu Asp Asp Asp Asp Asp Asp
        355                 360                


<210>    3
<211>    380
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CSQ2(full)


<400>    3
Glu Glu Gly Leu Asn Phe Pro Thr Tyr Asp Gly Lys Asp Arg Val Val
  1               5                  10                  15 

Ser Leu Ser Glu Lys Asn Phe Lys Gln Val Leu Lys Lys Tyr Asp Leu
             20                  25                  30 

Leu Cys Leu Tyr Tyr His Glu Pro Val Ser Ser Asp Lys Val Thr Pro
         35                  40                  45 

Lys Gln Phe Gln Leu Lys Glu Ile Val Leu Glu Leu Val Ala Gln Val
     50                  55                  60 

Leu Glu His Lys Ala Ile Gly Phe Val Met Val Asp Ala Lys Lys Glu
 65                  70                  75                  80 

Ala Lys Leu Ala Lys Lys Leu Gly Phe Asp Glu Glu Gly Ser Leu Tyr
                 85                  90                  95 

Ile Leu Lys Gly Asp Arg Thr Ile Glu Phe Asp Gly Glu Phe Ala Ala
            100                 105                 110 

Asp Val Leu Val Glu Phe Leu Leu Asp Leu Ile Glu Asp Pro Val Glu
        115                 120                 125 

Ile Ile Ser Ser Lys Leu Glu Val Gln Ala Phe Glu Arg Ile Glu Asp
    130                 135                 140 

Tyr Ile Lys Leu Ile Gly Phe Phe Lys Ser Glu Asp Ser Glu Tyr Tyr
145                 150                 155                 160 

Lys Ala Phe Glu Glu Ala Ala Glu His Phe Gln Pro Tyr Ile Lys Phe
                165                 170                 175 

Phe Ala Thr Phe Asp Lys Gly Val Ala Lys Lys Leu Ser Leu Lys Met
            180                 185                 190 

Asn Glu Val Asp Phe Tyr Glu Pro Phe Met Asp Glu Pro Ile Ala Ile
        195                 200                 205 

Pro Asn Lys Pro Tyr Thr Glu Glu Glu Leu Val Glu Phe Val Lys Glu
    210                 215                 220 

His Gln Arg Pro Thr Leu Arg Arg Leu Arg Pro Glu Glu Met Phe Glu
225                 230                 235                 240 

Thr Trp Glu Asp Asp Leu Asn Gly Ile His Ile Val Ala Phe Ala Glu
                245                 250                 255 

Lys Ser Asp Pro Asp Gly Tyr Glu Phe Leu Glu Ile Leu Lys Gln Val
            260                 265                 270 

Ala Arg Asp Asn Thr Asp Asn Pro Asp Leu Ser Ile Leu Trp Ile Asp
        275                 280                 285 

Pro Asp Asp Phe Pro Leu Leu Val Ala Tyr Trp Glu Lys Thr Phe Lys
    290                 295                 300 

Ile Asp Leu Phe Arg Pro Gln Ile Gly Val Val Asn Val Thr Asp Ala
305                 310                 315                 320 

Asp Ser Val Trp Met Glu Ile Pro Asp Asp Asp Asp Leu Pro Thr Ala
                325                 330                 335 

Glu Glu Leu Glu Asp Trp Ile Glu Asp Val Leu Ser Gly Lys Ile Asn
            340                 345                 350 

Thr Glu Asp Asp Asp Glu Asp Asp Asp Asp Asp Asp Asn Ser Asp Glu
        355                 360                 365 

Glu Asp Asn Asp Asp Ser Asp Asp Asp Asp Asp Glu
    370                 375                 380


<210>    4
<211>    58
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Z-DOMAIN


<400>    4
Val Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile
  1               5                  10                  15 

Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln
             20                  25                  30 

Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala
         35                  40                  45 

Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys
     50                  55            


<210>    5
<211>    116
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    ZZ


<400>    5
Val Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile
  1               5                  10                  15 

Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln
             20                  25                  30 

Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala
         35                  40                  45 

Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Val Asp Asn Lys Phe Asn
     50                  55                  60 

Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu
 65                  70                  75                  80 

Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys Asp Asp Pro
                 85                  90                  95 

Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala
            100                 105                 110 

Gln Ala Pro Lys
        115    


<210>    6
<211>    290
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Z(5)


<400>    6
Val Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile
  1               5                  10                  15 

Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln
             20                  25                  30 

Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala
         35                  40                  45 

Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Val Asp Asn Lys Phe Asn
     50                  55                  60 

Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu
 65                  70                  75                  80 

Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys Asp Asp Pro
                 85                  90                  95 

Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala
            100                 105                 110 

Gln Ala Pro Lys Val Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala
        115                 120                 125 

Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn
    130                 135                 140 

Ala Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu
145                 150                 155                 160 

Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Val Asp
                165                 170                 175 

Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His
            180                 185                 190 

Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu
        195                 200                 205 

Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys
    210                 215                 220 

Leu Asn Asp Ala Gln Ala Pro Lys Val Asp Asn Lys Phe Asn Lys Glu
225                 230                 235                 240 

Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Asn Glu
                245                 250                 255 

Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln
            260                 265                 270 

Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala
        275                 280                 285 

Pro Lys
    290


<210>    7
<211>    294
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Protein A


<400>    7
Ala Gln His Asp Glu Ala Gln Gln Asn Ala Phe Tyr Gln Val Leu Asn
  1               5                  10                  15 

Met Pro Asn Leu Asn Ala Asp Gln Arg Asn Gly Phe Ile Gln Ser Leu
             20                  25                  30 

Lys Asp Asp Pro Ser Gln Ser Ala Asn Val Leu Gly Glu Ala Gln Lys
         35                  40                  45 

Leu Asn Asp Ser Gln Ala Pro Lys Ala Asp Ala Gln Gln Asn Asn Phe
     50                  55                  60 

Asn Lys Asp Gln Gln Ser Ala Phe Tyr Glu Ile Leu Asn Met Pro Asn
 65                  70                  75                  80 

Leu Asn Glu Ala Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Asp Asp
                 85                  90                  95 

Pro Ser Gln Ser Thr Asn Val Leu Gly Glu Ala Lys Lys Leu Asn Glu
            100                 105                 110 

Ser Gln Ala Pro Lys Ala Asp Asn Asn Phe Asn Lys Glu Gln Gln Asn
        115                 120                 125 

Ala Phe Tyr Glu Ile Leu Asn Met Pro Asn Leu Asn Glu Glu Gln Arg
    130                 135                 140 

Asn Gly Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn
145                 150                 155                 160 

Leu Leu Ser Glu Ala Lys Lys Leu Asn Glu Ser Gln Ala Pro Lys Ala
                165                 170                 175 

Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu
            180                 185                 190 

His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser
        195                 200                 205 

Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys
    210                 215                 220 

Lys Leu Asn Asp Ala Gln Ala Pro Lys Ala Asp Asn Lys Phe Asn Lys
225                 230                 235                 240 

Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Thr
                245                 250                 255 

Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser
            260                 265                 270 

Val Ser Lys Glu Ile Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln
        275                 280                 285 

Ala Pro Lys Glu Glu Asp
    290                


<210>    8
<211>    200
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Protein G


<400>    8
Leu Pro Lys Thr Asp Thr Tyr Lys Leu Ile Leu Asn Gly Lys Thr Leu
  1               5                  10                  15 

Lys Gly Glu Thr Thr Thr Glu Ala Val Asp Ala Ala Thr Ala Glu Lys
             20                  25                  30 

Val Phe Lys Gln Tyr Ala Asn Asp Asn Gly Val Asp Gly Glu Trp Thr
         35                  40                  45 

Tyr Asp Asp Ala Thr Lys Thr Phe Thr Val Thr Glu Lys Pro Glu Val
     50                  55                  60 

Ile Asp Ala Ser Glu Leu Thr Pro Ala Val Thr Thr Tyr Lys Leu Val
 65                  70                  75                  80 

Ile Asn Gly Lys Thr Leu Lys Gly Glu Thr Thr Thr Glu Ala Val Asp
                 85                  90                  95 

Ala Ala Thr Ala Glu Lys Val Phe Lys Gln Tyr Ala Asn Asp Asn Gly
            100                 105                 110 

Val Asp Gly Glu Trp Thr Tyr Asp Asp Ala Thr Lys Thr Phe Thr Val
        115                 120                 125 

Thr Glu Lys Pro Glu Val Ile Asp Ala Ser Glu Leu Thr Pro Ala Val
    130                 135                 140 

Thr Thr Tyr Lys Leu Val Ile Asn Gly Lys Thr Leu Lys Gly Glu Thr
145                 150                 155                 160 

Thr Thr Lys Ala Val Asp Ala Glu Thr Ala Glu Lys Ala Phe Lys Gln
                165                 170                 175 

Tyr Ala Asn Asp Asn Gly Val Asp Gly Val Trp Thr Tyr Asp Asp Ala
            180                 185                 190 

Thr Lys Thr Phe Thr Val Thr Glu
        195                 200


<210>    9
<211>    427
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Protein A/G


<400>    9
Ala Gln His Asp Glu Ala Gln Gln Asn Ala Phe Tyr Gln Val Leu Asn
  1               5                  10                  15 

Met Pro Asn Leu Asn Ala Asp Gln Arg Asn Gly Phe Ile Gln Ser Leu
             20                  25                  30 

Lys Asp Asp Pro Ser Gln Ser Ala Asn Val Leu Gly Glu Ala Gln Lys
         35                  40                  45 

Leu Asn Asp Ser Gln Ala Pro Lys Ala Asp Ala Gln Gln Asn Asn Phe
     50                  55                  60 

Asn Lys Asp Gln Gln Ser Ala Phe Tyr Glu Ile Leu Asn Met Pro Asn
 65                  70                  75                  80 

Leu Asn Glu Ala Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Asp Asp
                 85                  90                  95 

Pro Ser Gln Ser Thr Asn Val Leu Gly Glu Ala Lys Lys Leu Asn Glu
            100                 105                 110 

Ser Gln Ala Pro Lys Ala Asp Asn Asn Phe Asn Lys Glu Gln Gln Asn
        115                 120                 125 

Ala Phe Tyr Glu Ile Leu Asn Met Pro Asn Leu Asn Glu Glu Gln Arg
    130                 135                 140 

Asn Gly Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn
145                 150                 155                 160 

Leu Leu Ser Glu Ala Lys Lys Leu Asn Glu Ser Gln Ala Pro Lys Ala
                165                 170                 175 

Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu
            180                 185                 190 

His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser
        195                 200                 205 

Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys
    210                 215                 220 

Lys Leu Asn Asp Ala Gln Ala Pro Lys Ala Asp Asn Lys Phe Asn Lys
225                 230                 235                 240 

Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Thr
                245                 250                 255 

Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser
            260                 265                 270 

Val Ser Lys Glu Ile Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln
        275                 280                 285 

Ala Pro Lys Glu Glu Asp Ser Leu Glu Gly Ser Gly Ser Gly Thr Tyr
    290                 295                 300 

Lys Leu Ile Leu Asn Gly Lys Thr Leu Lys Gly Glu Thr Thr Thr Glu
305                 310                 315                 320 

Ala Val Asp Ala Ala Thr Ala Glu Lys Val Phe Lys Gln Tyr Ala Asn
                325                 330                 335 

Asp Asn Gly Val Asp Gly Glu Trp Thr Tyr Asp Asp Ala Thr Lys Thr
            340                 345                 350 

Phe Thr Val Thr Glu Lys Pro Glu Val Ile Asp Ala Ser Glu Leu Thr
        355                 360                 365 

Pro Ala Val Thr Thr Tyr Lys Leu Val Ile Asn Gly Lys Thr Leu Lys
    370                 375                 380 

Gly Glu Thr Thr Thr Lys Ala Val Asp Ala Glu Thr Ala Glu Lys Ala
385                 390                 395                 400 

Phe Lys Gln Tyr Ala Asn Asp Asn Gly Val Asp Gly Val Trp Thr Tyr
                405                 410                 415 

Asp Asp Ala Thr Lys Thr Phe Thr Val Thr Glu
            420                 425        
