                         SEQUENCE LISTING

<110>  LAMKAP BIO BETA AG
       BUATOIS, VANESSA
       HOSE, DIRK
       SECKINGER, ANJA
 
<120>  BISPECIFIC ANTIBODIES AGAINST CEACAM5 AND CD47

<130>  4130.005PC03

<150>  US 63/135,996
<151>  2021-01-11

<150>  EP 20215766
<151>  2020-12-18

<160>  50    

<170>  PatentIn version 3.5

<210>  1
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Common heavy chain CDRH1

<400>  1

Ser Tyr Ala Met Ser 
1               5   


<210>  2
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Common heavy chain CDRH2

<400>  2

Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  3
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Common heavy chain CDRH3

<400>  3

Ser Tyr Gly Ala Phe Asp Tyr 
1               5           


<210>  4
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Common heavy chain VH

<400>  4

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Ser Tyr Gly Ala Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  5
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Common heavy chain (VH-CH1)

<400>  5

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Ser Tyr Gly Ala Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala 
        115                 120                 125             


Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu 
    130                 135                 140                 


Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly 
145                 150                 155                 160 


Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser 
                165                 170                 175     


Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu 
            180                 185                 190         


Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr 
        195                 200                 205             


Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr 
    210                 215                 220                 


Cys Pro Pro Cys Pro 
225                 


<210>  6
<211>  445
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  common heavy chain (VH-CH1-CH2-CH3)

<400>  6

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Ser Tyr Gly Ala Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala 
        115                 120                 125             


Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu 
    130                 135                 140                 


Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly 
145                 150                 155                 160 


Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser 
                165                 170                 175     


Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu 
            180                 185                 190         


Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr 
        195                 200                 205             


Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr 
    210                 215                 220                 


Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 
225                 230                 235                 240 


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
                245                 250                 255     


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
            260                 265                 270         


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
        275                 280                 285             


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
    290                 295                 300                 


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
305                 310                 315                 320 


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
                325                 330                 335     


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
            340                 345                 350         


Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
        355                 360                 365             


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
    370                 375                 380                 


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
385                 390                 395                 400 


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
                405                 410                 415     


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
            420                 425                 430         


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
        435                 440                 445 


<210>  7
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD47 binding part CDRL1

<400>  7

Arg Ala Ser Gln Ser Ile Ser Ser Tyr Leu Asn 
1               5                   10      


<210>  8
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD47 binding part CDRL2

<400>  8

Ala Ala Ser Ser Leu Gln Ser 
1               5           


<210>  9
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD47 binding part CDRL3

<400>  9

Gln Gln Met His Pro Arg Ala Pro Lys Thr 
1               5                   10  


<210>  10
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD47 binding part VK

<400>  10

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Ser Ser Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Met His Pro Arg Ala Pro 
                85                  90                  95      


Lys Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105             


<210>  11
<211>  215
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD47 binding part light chain (VKCK; K2)

<400>  11

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Ser Ser Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Met His Pro Arg Ala Pro 
                85                  90                  95      


Lys Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala 
            100                 105                 110         


Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser 
        115                 120                 125             


Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu 
    130                 135                 140                 


Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser 
145                 150                 155                 160 


Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu 
                165                 170                 175     


Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val 
            180                 185                 190         


Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys 
        195                 200                 205             


Ser Phe Asn Arg Gly Glu Cys 
    210                 215 


<210>  12
<211>  648
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD47 binding part light chain (VKCK; nucleic acid); (K2)

<400>  12
gacatccaga tgacccagtc tccatcctcc ctgtctgcat ctgtaggaga cagagtcacc       60

atcacttgcc gggcaagtca gagcattagc agctatttaa attggtatca gcagaaacca      120

gggaaagccc ctaagctcct gatctatgct gcatccagtt tgcaaagtgg ggtcccatca      180

aggttcagtg gcagtggatc tgggacagat ttcactctca ccatcagcag tctgcaacct      240

gaagattttg caacttacta ctgtcagcag atgcacccgc gcgccccgaa gaccttcggc      300

caagggacca aggtggaaat caaacgtacg gtggctgcac catctgtctt catcttcccg      360

ccatctgatg agcagttgaa atctggaact gcctctgttg tgtgcctgct gaataacttc      420

tatcccagag aggccaaagt acagtggaag gtggataacg ccctccaatc gggtaactcc      480

caggagagtg tcacagagca ggacagcaag gacagcacct acagcctcag cagcaccctg      540

acgctgagca aagcagacta cgagaaacac aaagtctacg cctgcgaagt cacccatcag      600

ggcctgagct cgcccgtcac aaagagcttc aacaggggag agtgttaa                   648


<210>  13
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD47 binding part constant light chain kappa (CK)

<400>  13

Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         


<210>  14
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CEACAM5 binding part AC82 CDRL1;

<400>  14

Ser Gly Ser Ser Ser Asn Ile Gly Tyr Gly Leu Val Ser 
1               5                   10              


<210>  15
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CEACAM5 binding part AC82 CDRL2

<400>  15

Gly Ile Asn Lys Arg Pro Ser 
1               5           


<210>  16
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CEACAM5 binding part AC82 CDRL3

<400>  16

Gly Thr Trp Asp Phe Ser Tyr Arg Val Asp 
1               5                   10  


<210>  17
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CEACAM5 binding part AC84 CDRL1

<400>  17

Ser Gly Ser Ser Ser Asn Ile Gly Ile Gly Leu Val Ser 
1               5                   10              


<210>  18
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CEACAM5 binding part AC84 CDRL2

<400>  18

Asn Val Asn Thr Arg Pro Ser 
1               5           


<210>  19
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CEACAM5 binding part AC84 CDRL3

<400>  19

Gly Thr Trp Asp Phe Ser Tyr Arg Val Asp 
1               5                   10  


<210>  20
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CEACAM5 binding part AC91 CDRL1

<400>  20

Ser Gly Ser Ser Ser Asn Ile Gly Ile Gly Leu Val Ser 
1               5                   10              


<210>  21
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CEACAM5 binding part AC91 CDRL2

<400>  21

Thr Val Asn Asn Arg Pro Ser 
1               5           


<210>  22
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CEACAM5 binding part AC91 CDRL3

<400>  22

Gly Thr Phe Asp Phe Ser Tyr Gly Ile Val 
1               5                   10  


<210>  23
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CEACAM5 binding part AC100 CDRL1

<400>  23

Ser Gly Ser Ser Ser Asn Ile Gly Tyr Gly Leu Val Ser 
1               5                   10              


<210>  24
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CEACAM5 binding part AC100 CDRL2

<400>  24

Asn Gly Asn Ile Arg Pro Ser 
1               5           


<210>  25
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CEACAM5 binding part AC100 CDRL3

<400>  25

Gly Thr Trp Asp Phe Ser Tyr Arg Val Asp 
1               5                   10  


<210>  26
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CEACAM5 binding part AC117 CDRL1

<400>  26

Ser Gly Ser Ser Ser Asn Ile Gly Ile Gly Leu Val Ser 
1               5                   10              


<210>  27
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CEACAM5 binding part AC117 CDRL2

<400>  27

Asn Gly Asn Val Arg Pro Ser 
1               5           


<210>  28
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CEACAM5 binding part AC117 CDRL3

<400>  28

Gly Thr Trp Asp Phe Ser Tyr Arg Val Asp 
1               5                   10  


<210>  29
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CEACAM5 binding part AC22 CDRL1

<400>  29

Ser Gly Ser Ser Ser Asn Ile Ala Asn Gly Ile Val Ser 
1               5                   10              


<210>  30
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CEACAM5 binding part AC22 CDRL2

<400>  30

Phe Asp Asn Leu Arg Pro Ser 
1               5           


<210>  31
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CEACAM5 binding part AC22 CDRL3

<400>  31

Gly Thr Trp Asp Phe Ser Tyr Gly Ile Val 
1               5                   10  


<210>  32
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CEACAM5 binding part AC82 VL

<400>  32

Gln Ser Val Leu Thr Gln Pro Pro Ser Val Ser Ala Ala Pro Gly Gln 
1               5                   10                  15      


Lys Val Thr Ile Ser Cys Ser Gly Ser Ser Ser Asn Ile Gly Tyr Gly 
            20                  25                  30          


Leu Val Ser Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu 
        35                  40                  45              


Ile Tyr Gly Ile Asn Lys Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Lys Ser Gly Thr Ser Ala Thr Leu Gly Ile Thr Gly Leu Gln 
65                  70                  75                  80  


Thr Gly Asp Glu Ala Asp Tyr Tyr Cys Gly Thr Trp Asp Phe Ser Tyr 
                85                  90                  95      


Arg Val Asp Phe Gly Gly Gly Thr Lys Leu Thr Val Leu 
            100                 105                 


<210>  33
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CEACAM5 binding part AC84 VL

<400>  33

Gln Ser Val Leu Thr Gln Pro Pro Ser Val Ser Ala Ala Pro Gly Gln 
1               5                   10                  15      


Lys Val Thr Ile Ser Cys Ser Gly Ser Ser Ser Asn Ile Gly Ile Gly 
            20                  25                  30          


Leu Val Ser Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu 
        35                  40                  45              


Ile Tyr Asn Val Asn Thr Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Lys Ser Gly Thr Ser Ala Thr Leu Gly Ile Thr Gly Leu Gln 
65                  70                  75                  80  


Thr Gly Asp Glu Ala Asp Tyr Tyr Cys Gly Thr Trp Asp Phe Ser Tyr 
                85                  90                  95      


Arg Val Asp Phe Gly Gly Gly Thr Lys Leu Thr Val Leu 
            100                 105                 


<210>  34
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CEACAM5 binding part AC91 VL

<400>  34

Gln Ser Val Leu Thr Gln Pro Pro Ser Val Ser Ala Ala Pro Gly Gln 
1               5                   10                  15      


Lys Val Thr Ile Ser Cys Ser Gly Ser Ser Ser Asn Ile Gly Ile Gly 
            20                  25                  30          


Leu Val Ser Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu 
        35                  40                  45              


Ile Tyr Thr Val Asn Asn Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Lys Ser Gly Thr Ser Ala Thr Leu Gly Ile Thr Gly Leu Gln 
65                  70                  75                  80  


Thr Gly Asp Glu Ala Asp Tyr Tyr Cys Gly Thr Phe Asp Phe Ser Tyr 
                85                  90                  95      


Gly Ile Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu 
            100                 105                 


<210>  35
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CEACAM5 binding part AC100 VL

<400>  35

Gln Ser Val Leu Thr Gln Pro Pro Ser Val Ser Ala Ala Pro Gly Gln 
1               5                   10                  15      


Lys Val Thr Ile Ser Cys Ser Gly Ser Ser Ser Asn Ile Gly Tyr Gly 
            20                  25                  30          


Leu Val Ser Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu 
        35                  40                  45              


Ile Tyr Asn Gly Asn Ile Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Lys Ser Gly Thr Ser Ala Thr Leu Gly Ile Thr Gly Leu Gln 
65                  70                  75                  80  


Thr Gly Asp Glu Ala Asp Tyr Tyr Cys Gly Thr Trp Asp Phe Ser Tyr 
                85                  90                  95      


Arg Val Asp Phe Gly Gly Gly Thr Lys Leu Thr Val Leu 
            100                 105                 


<210>  36
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CEACAM5 binding part AC117 VL

<400>  36

Gln Ser Val Leu Thr Gln Pro Pro Ser Val Ser Ala Ala Pro Gly Gln 
1               5                   10                  15      


Lys Val Thr Ile Ser Cys Ser Gly Ser Ser Ser Asn Ile Gly Ile Gly 
            20                  25                  30          


Leu Val Ser Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu 
        35                  40                  45              


Ile Tyr Asn Gly Asn Val Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Lys Ser Gly Thr Ser Ala Thr Leu Gly Ile Thr Gly Leu Gln 
65                  70                  75                  80  


Thr Gly Asp Glu Ala Asp Tyr Tyr Cys Gly Thr Trp Asp Phe Ser Tyr 
                85                  90                  95      


Arg Val Asp Phe Gly Gly Gly Thr Lys Leu Thr Val Leu 
            100                 105                 


<210>  37
<211>  215
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CEACAM5 binding part AC82 VLCL

<400>  37

Gln Ser Val Leu Thr Gln Pro Pro Ser Val Ser Ala Ala Pro Gly Gln 
1               5                   10                  15      


Lys Val Thr Ile Ser Cys Ser Gly Ser Ser Ser Asn Ile Gly Tyr Gly 
            20                  25                  30          


Leu Val Ser Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu 
        35                  40                  45              


Ile Tyr Gly Ile Asn Lys Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Lys Ser Gly Thr Ser Ala Thr Leu Gly Ile Thr Gly Leu Gln 
65                  70                  75                  80  


Thr Gly Asp Glu Ala Asp Tyr Tyr Cys Gly Thr Trp Asp Phe Ser Tyr 
                85                  90                  95      


Arg Val Asp Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gln Pro 
            100                 105                 110         


Lys Ala Ala Pro Ser Val Thr Leu Phe Pro Pro Ser Ser Glu Glu Leu 
        115                 120                 125             


Gln Ala Asn Lys Ala Thr Leu Val Cys Leu Ile Ser Asp Phe Tyr Pro 
    130                 135                 140                 


Gly Ala Val Thr Val Ala Trp Lys Ala Asp Ser Ser Pro Val Lys Ala 
145                 150                 155                 160 


Gly Val Glu Thr Thr Thr Pro Ser Lys Gln Ser Asn Asn Lys Tyr Ala 
                165                 170                 175     


Ala Ser Ser Tyr Leu Ser Leu Thr Pro Glu Gln Trp Lys Ser His Arg 
            180                 185                 190         


Ser Tyr Ser Cys Gln Val Thr His Glu Gly Ser Thr Val Glu Lys Thr 
        195                 200                 205             


Val Ala Pro Thr Glu Cys Ser 
    210                 215 


<210>  38
<211>  215
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CEACAM5 binding part AC84 VLCL

<400>  38

Gln Ser Val Leu Thr Gln Pro Pro Ser Val Ser Ala Ala Pro Gly Gln 
1               5                   10                  15      


Lys Val Thr Ile Ser Cys Ser Gly Ser Ser Ser Asn Ile Gly Ile Gly 
            20                  25                  30          


Leu Val Ser Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu 
        35                  40                  45              


Ile Tyr Asn Val Asn Thr Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Lys Ser Gly Thr Ser Ala Thr Leu Gly Ile Thr Gly Leu Gln 
65                  70                  75                  80  


Thr Gly Asp Glu Ala Asp Tyr Tyr Cys Gly Thr Trp Asp Phe Ser Tyr 
                85                  90                  95      


Arg Val Asp Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gln Pro 
            100                 105                 110         


Lys Ala Ala Pro Ser Val Thr Leu Phe Pro Pro Ser Ser Glu Glu Leu 
        115                 120                 125             


Gln Ala Asn Lys Ala Thr Leu Val Cys Leu Ile Ser Asp Phe Tyr Pro 
    130                 135                 140                 


Gly Ala Val Thr Val Ala Trp Lys Ala Asp Ser Ser Pro Val Lys Ala 
145                 150                 155                 160 


Gly Val Glu Thr Thr Thr Pro Ser Lys Gln Ser Asn Asn Lys Tyr Ala 
                165                 170                 175     


Ala Ser Ser Tyr Leu Ser Leu Thr Pro Glu Gln Trp Lys Ser His Arg 
            180                 185                 190         


Ser Tyr Ser Cys Gln Val Thr His Glu Gly Ser Thr Val Glu Lys Thr 
        195                 200                 205             


Val Ala Pro Thr Glu Cys Ser 
    210                 215 


<210>  39
<211>  215
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CEACAM5 binding part AC91 VLCL

<400>  39

Gln Ser Val Leu Thr Gln Pro Pro Ser Val Ser Ala Ala Pro Gly Gln 
1               5                   10                  15      


Lys Val Thr Ile Ser Cys Ser Gly Ser Ser Ser Asn Ile Gly Ile Gly 
            20                  25                  30          


Leu Val Ser Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu 
        35                  40                  45              


Ile Tyr Thr Val Asn Asn Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Lys Ser Gly Thr Ser Ala Thr Leu Gly Ile Thr Gly Leu Gln 
65                  70                  75                  80  


Thr Gly Asp Glu Ala Asp Tyr Tyr Cys Gly Thr Phe Asp Phe Ser Tyr 
                85                  90                  95      


Gly Ile Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gln Pro 
            100                 105                 110         


Lys Ala Ala Pro Ser Val Thr Leu Phe Pro Pro Ser Ser Glu Glu Leu 
        115                 120                 125             


Gln Ala Asn Lys Ala Thr Leu Val Cys Leu Ile Ser Asp Phe Tyr Pro 
    130                 135                 140                 


Gly Ala Val Thr Val Ala Trp Lys Ala Asp Ser Ser Pro Val Lys Ala 
145                 150                 155                 160 


Gly Val Glu Thr Thr Thr Pro Ser Lys Gln Ser Asn Asn Lys Tyr Ala 
                165                 170                 175     


Ala Ser Ser Tyr Leu Ser Leu Thr Pro Glu Gln Trp Lys Ser His Arg 
            180                 185                 190         


Ser Tyr Ser Cys Gln Val Thr His Glu Gly Ser Thr Val Glu Lys Thr 
        195                 200                 205             


Val Ala Pro Thr Glu Cys Ser 
    210                 215 


<210>  40
<211>  215
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CEACAM5 binding part AC100 VLCL

<400>  40

Gln Ser Val Leu Thr Gln Pro Pro Ser Val Ser Ala Ala Pro Gly Gln 
1               5                   10                  15      


Lys Val Thr Ile Ser Cys Ser Gly Ser Ser Ser Asn Ile Gly Tyr Gly 
            20                  25                  30          


Leu Val Ser Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu 
        35                  40                  45              


Ile Tyr Asn Gly Asn Ile Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Lys Ser Gly Thr Ser Ala Thr Leu Gly Ile Thr Gly Leu Gln 
65                  70                  75                  80  


Thr Gly Asp Glu Ala Asp Tyr Tyr Cys Gly Thr Trp Asp Phe Ser Tyr 
                85                  90                  95      


Arg Val Asp Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gln Pro 
            100                 105                 110         


Lys Ala Ala Pro Ser Val Thr Leu Phe Pro Pro Ser Ser Glu Glu Leu 
        115                 120                 125             


Gln Ala Asn Lys Ala Thr Leu Val Cys Leu Ile Ser Asp Phe Tyr Pro 
    130                 135                 140                 


Gly Ala Val Thr Val Ala Trp Lys Ala Asp Ser Ser Pro Val Lys Ala 
145                 150                 155                 160 


Gly Val Glu Thr Thr Thr Pro Ser Lys Gln Ser Asn Asn Lys Tyr Ala 
                165                 170                 175     


Ala Ser Ser Tyr Leu Ser Leu Thr Pro Glu Gln Trp Lys Ser His Arg 
            180                 185                 190         


Ser Tyr Ser Cys Gln Val Thr His Glu Gly Ser Thr Val Glu Lys Thr 
        195                 200                 205             


Val Ala Pro Thr Glu Cys Ser 
    210                 215 


<210>  41
<211>  215
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CEACAM5 binding part AC117 VLCL

<400>  41

Gln Ser Val Leu Thr Gln Pro Pro Ser Val Ser Ala Ala Pro Gly Gln 
1               5                   10                  15      


Lys Val Thr Ile Ser Cys Ser Gly Ser Ser Ser Asn Ile Gly Ile Gly 
            20                  25                  30          


Leu Val Ser Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu 
        35                  40                  45              


Ile Tyr Asn Gly Asn Val Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Lys Ser Gly Thr Ser Ala Thr Leu Gly Ile Thr Gly Leu Gln 
65                  70                  75                  80  


Thr Gly Asp Glu Ala Asp Tyr Tyr Cys Gly Thr Trp Asp Phe Ser Tyr 
                85                  90                  95      


Arg Val Asp Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gln Pro 
            100                 105                 110         


Lys Ala Ala Pro Ser Val Thr Leu Phe Pro Pro Ser Ser Glu Glu Leu 
        115                 120                 125             


Gln Ala Asn Lys Ala Thr Leu Val Cys Leu Ile Ser Asp Phe Tyr Pro 
    130                 135                 140                 


Gly Ala Val Thr Val Ala Trp Lys Ala Asp Ser Ser Pro Val Lys Ala 
145                 150                 155                 160 


Gly Val Glu Thr Thr Thr Pro Ser Lys Gln Ser Asn Asn Lys Tyr Ala 
                165                 170                 175     


Ala Ser Ser Tyr Leu Ser Leu Thr Pro Glu Gln Trp Lys Ser His Arg 
            180                 185                 190         


Ser Tyr Ser Cys Gln Val Thr His Glu Gly Ser Thr Val Glu Lys Thr 
        195                 200                 205             


Val Ala Pro Thr Glu Cys Ser 
    210                 215 


<210>  42
<211>  215
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CEACAM5 binding part AC22 VLCL

<400>  42

Gln Ser Val Leu Thr Gln Pro Pro Ser Val Ser Ala Ala Pro Gly Gln 
1               5                   10                  15      


Lys Val Thr Ile Ser Cys Ser Gly Ser Ser Ser Asn Ile Ala Asn Gly 
            20                  25                  30          


Ile Val Ser Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu 
        35                  40                  45              


Ile Tyr Phe Asp Asn Leu Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Lys Ser Gly Thr Ser Ala Thr Leu Gly Ile Thr Gly Leu Gln 
65                  70                  75                  80  


Thr Gly Asp Glu Ala Asp Tyr Tyr Cys Gly Thr Trp Asp Phe Ser Tyr 
                85                  90                  95      


Gly Ile Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gln Pro 
            100                 105                 110         


Lys Ala Ala Pro Ser Val Thr Leu Phe Pro Pro Ser Ser Glu Glu Leu 
        115                 120                 125             


Gln Ala Asn Lys Ala Thr Leu Val Cys Leu Ile Ser Asp Phe Tyr Pro 
    130                 135                 140                 


Gly Ala Val Thr Val Ala Trp Lys Ala Asp Ser Ser Pro Val Lys Ala 
145                 150                 155                 160 


Gly Val Glu Thr Thr Thr Pro Ser Lys Gln Ser Asn Asn Lys Tyr Ala 
                165                 170                 175     


Ala Ser Ser Tyr Leu Ser Leu Thr Pro Glu Gln Trp Lys Ser His Arg 
            180                 185                 190         


Ser Tyr Ser Cys Gln Val Thr His Glu Gly Ser Thr Val Glu Lys Thr 
        195                 200                 205             


Val Ala Pro Thr Glu Cys Ser 
    210                 215 


<210>  43
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VK_SM3E

<400>  43

Glu Asn Val Leu Thr Gln Ser Pro Ser Ser Met Ser Val Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Asn Ile Ala Cys Ser Ala Ser Ser Ser Val Pro Tyr Met 
            20                  25                  30          


His Trp Leu Gln Gln Lys Pro Gly Lys Ser Pro Lys Leu Leu Ile Tyr 
        35                  40                  45              


Leu Thr Ser Asn Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Ser Val Gln Pro Glu 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Arg Ser Ser Tyr Pro Leu Thr 
                85                  90                  95      


Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105     


<210>  44
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH_SM3E

<400>  44

Gln Val Lys Leu Glu Gln Ser Gly Ala Glu Val Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Phe Asn Ile Lys Asp Ser 
            20                  25                  30          


Tyr Met His Trp Leu Arg Gln Gly Pro Gly Gln Arg Leu Glu Trp Ile 
        35                  40                  45              


Gly Trp Ile Asp Pro Glu Asn Gly Asp Thr Glu Tyr Ala Pro Lys Phe 
    50                  55                  60                  


Gln Gly Lys Ala Thr Phe Thr Thr Asp Thr Ser Ala Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gly Leu Ser Ser Leu Arg Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Asn Glu Gly Thr Pro Thr Gly Pro Tyr Tyr Phe Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser 
        115                 120 


<210>  45
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  45
gtctgaagca ttatgtgttg aagc                                              24


<210>  46
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  46
gtgagtacat tcattgtact gtg                                               23


<210>  47
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  47
ttgtgtgact cttaactctc agag                                              24


<210>  48
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  48
gaggccactt gtgtagcgcc aagtg                                             25


<210>  49
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  49
gtgagtccat ggctgtcact g                                                 21


<210>  50
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  50
cctgacttgg ctattctcag                                                   20


