               SEQUENCE LISTING

<110> Universitat Pompeu Fabra

<120> THERAPEUTIC LAMA2 PAYLOAD FOR TREATMENT OF CONGENITAL MUSCULAR DYSTROPHY

<130> IBIO-2200/PCT

<150> EP21209721.6
<151> 2021-11-22
<150> EP20214691.6
<151> 2020-12-16

<160> 134

<170> BiSSAP 1.3.6

<210> 1
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac

<400> 1
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Arg Gly Arg Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Ala Ser Asn Ala Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asn Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560 
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Ser Ala Ser Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser 
            580                 585                 590         
Cys Phe 
        

<210> 2
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac

<400> 2
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Arg Gly Arg Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ala Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Ala Ser Asn Ala Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Ala Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asn Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Ala Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560 
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Ser Ala Ala Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Val Cys Gln Gly 
            580                 585                 590         
Cys Leu 
        

<210> 3
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac

<400> 3
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Ala Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Ala Gly Ala Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Ala Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Ala Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560 
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Ser Ala Ser Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Val Cys Gln Ser 
            580                 585                 590         
Cys Phe 
        

<210> 4
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac

<400> 4
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Ala Gly Arg Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Ala Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Ala Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Ala 
545                 550                 555                 560 
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Ser Ala Ser Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser 
            580                 585                 590         
Cys Phe 
        

<210> 5
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac

<400> 5
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Arg Gly Arg Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ala Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Ala Ser Asn Ala Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Ala Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asn Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Ala Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560 
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Ser Ala Ala Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Val Cys Gln Arg 
            580                 585                 590         
Cys Leu 
        

<210> 6
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac

<400> 6
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Ala Gly Ala Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Ser Trp Phe Thr Ser Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Arg Ser Asn Ala Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Ala Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asn Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Ala Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Ala 
545                 550                 555                 560 
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Ser Ala Ala Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Val Cys Gln Ser 
            580                 585                 590         
Cys Leu 
        

<210> 7
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac

<400> 7
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Ala Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Ala Gly Ala Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Ala Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Ala Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560 
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Ser Ala Ser Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser 
            580                 585                 590         
Cys Phe 
        

<210> 8
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac

<400> 8
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Arg Gly Arg Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Ala Ser Asn Ala Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Ala Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asn Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Ala Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560 
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Ser Ala Ala Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Val Cys Gln Arg 
            580                 585                 590         
Cys Leu 
        

<210> 9
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Hyperactive PiggyBac aa sequence

<400> 9
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Arg Gly Arg Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560 
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Ser Ala Ser Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser 
            580                 585                 590         
Cys Phe 
        

<210> 10
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac

<220> 
<221> SITE
<222> 245..245
<223> Wherein amino acid can be Arg, Ala

<220> 
<221> SITE
<222> 268..268
<223> Wherein amino acid can be Asp, Asn

<220> 
<221> SITE
<222> 275..275
<223> Wherein amino acid can be Arg, Ala

<220> 
<221> SITE
<222> 277..277
<223> Wherein amino acid can be Arg, Ala

<220> 
<221> SITE
<222> 287..287
<223> Wherein amino acid can be Ala, Lys

<220> 
<221> SITE
<222> 290..290
<223> Wherein amino acid can be Ala, Lys

<220> 
<221> SITE
<222> 315..315
<223> Wherein amino acid can be Arg, Ala

<220> 
<221> SITE
<222> 325..325
<223> Wherein amino acid can be Gly, Ala

<220> 
<221> SITE
<222> 341..341
<223> Wherein amino acid can be Arg, Ala

<220> 
<221> SITE
<222> 346..346
<223> Wherein amino acid can be Asp, Asn

<220> 
<221> SITE
<222> 347..347
<223> Wherein amino acid can be Asn, Ala, Ser

<220> 
<221> SITE
<222> 350..350
<223> Wherein amino acid can be Thr, Ala

<220> 
<221> SITE
<222> 351..351
<223> Xaa can be Ser, Glu, Pro, Ala

<220> 
<221> SITE
<222> 356..356
<223> Wherein amino acid can be Lys, Glu

<220> 
<221> SITE
<222> 357..357
<223> Wherein amino acid can be Asn, Ala

<220> 
<221> SITE
<222> 372..372
<223> Wherein amino acid can be Arg, Ala

<220> 
<221> SITE
<222> 375..375
<223> Wherein amino acid can be Lys, Ala

<220> 
<221> SITE
<222> 388..388
<223> Wherein amino acid can be Arg, Ala

<220> 
<221> SITE
<222> 409..409
<223> Wherein amino acid can be Lys, Ala

<220> 
<221> SITE
<222> 412..412
<223> Wherein amino acid can be Lys, Ala

<220> 
<221> SITE
<222> 432..432
<223> Wherein amino acid can be Lys, Ala

<220> 
<221> SITE
<222> 460..460
<223> Wherein amino acid can be Arg, Ala

<220> 
<221> SITE
<222> 461..461
<223> Wherein amino acid can be Ala, Lys

<220> 
<221> SITE
<222> 465..465
<223> Wherein amino acid can be Trp, Ala

<220> 
<221> SITE
<222> 560..560
<223> Wherein amino acid can be Thr, Ala

<220> 
<221> SITE
<222> 564..564
<223> Wherein amino acid can be Ser, Pro

<220> 
<221> SITE
<222> 571..571
<223> Wherein amino acid can be Asn, Ser

<220> 
<221> SITE
<222> 573..573
<223> Wherein amino acid can be Ser, Ala

<220> 
<221> SITE
<222> 576..576
<223> Wherein amino acid can be Lys, Ala

<220> 
<221> SITE
<222> 586..586
<223> Wherein amino acid can be His, any naturally occuring amino acid

<220> 
<221> SITE
<222> 587..587
<223> Wherein amino acid can be Ile, Ala

<220> 
<221> SITE
<222> 589..589
<223> Wherein amino acid can be Met, Val

<220> 
<221> SITE
<222> 592..592
<223> Wherein amino acid can be Ser, Gly

<220> 
<221> SITE
<222> 594..594
<223> Wherein amino acid can be Phe, Leu

<400> 10
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Xaa Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Xaa Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Xaa Gly Xaa Cys Pro Phe Arg Val Tyr Ile Pro Asn Xaa Pro 
        275                 280                 285             
Ser Xaa Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Xaa Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Xaa Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Xaa Asn Ile Thr Cys Xaa Xaa Trp Phe Xaa Xaa Ile 
            340                 345                 350         
Pro Leu Ala Xaa Xaa Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Xaa Ser Asn Xaa Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Xaa Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Xaa Pro Ala Xaa Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Xaa 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Xaa Xaa Thr Asn Arg 
    450                 455                 460                 
Xaa Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Xaa 
545                 550                 555                 560 
Tyr Cys Pro Xaa Lys Ile Arg Arg Lys Ala Xaa Ala Xaa Cys Lys Xaa 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Xaa Xaa Asp Xaa Cys Gln Xaa 
            580                 585                 590         
Cys Xaa 
        

<210> 11
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac with mutations in the catalytic
      triad

<220> 
<221> SITE
<222> 268..268
<223> Xaa can be Asp, Asn

<220> 
<221> SITE
<222> 346..346
<223> Xaa can be Asp, Asn

<400> 11
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Xaa Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Arg Gly Arg Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Xaa Asn Trp Phe Thr Ser Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560 
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Ser Ala Ser Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser 
            580                 585                 590         
Cys Phe 
        

<210> 12
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac with mutations in amino acids that
      are critical for excision

<220> 
<221> SITE
<222> 287..287
<223> Xaa can be Lys, Ala

<220> 
<221> SITE
<222> 290..290
<223> Xaa can be Lys, Ala

<220> 
<221> SITE
<222> 460..460
<223> Xaa can be Arg, Ala

<220> 
<221> SITE
<222> 461..461
<223> Xaa can be Lys, Ala

<400> 12
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Arg Gly Arg Cys Pro Phe Arg Val Tyr Ile Pro Asn Xaa Pro 
        275                 280                 285             
Ser Xaa Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Xaa Xaa Thr Asn Arg 
    450                 455                 460                 
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560 
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Ser Ala Ser Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser 
            580                 585                 590         
Cys Phe 
        

<210> 13
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac with mutation that are involved in
      target joining

<220> 
<221> SITE
<222> 351..351
<223> Xaa can be Ser, Glu, Pro, Ala

<220> 
<221> SITE
<222> 356..356
<223> Xaa can be Lys, Glu

<400> 13
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Arg Gly Arg Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Xaa Ile 
            340                 345                 350         
Pro Leu Ala Xaa Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560 
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Ser Ala Ser Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser 
            580                 585                 590         
Cys Phe 
        

<210> 14
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac with mutations that are critical
      for integration

<220> 
<221> SITE
<222> 560..560
<223> Wherein amino acid can be Thr, Ala,

<220> 
<221> SITE
<222> 564..564
<223> Wherein amino acid can be Ser, Pro

<220> 
<221> SITE
<222> 571..571
<223> Wherein amino acid can be Asn, Ser

<220> 
<221> SITE
<222> 573..573
<223> Wherein amino acid can be Ser, Ala

<220> 
<221> SITE
<222> 589..589
<223> Wherein amino acid can be Met, Val

<220> 
<221> SITE
<222> 592..592
<223> Wherein amino acid can be Ser, Gly

<220> 
<221> SITE
<222> 594..594
<223> Wherein amino acid can be Phe, Leu

<400> 14
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Arg Gly Arg Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Xaa 
545                 550                 555                 560 
Tyr Cys Pro Xaa Lys Ile Arg Arg Lys Ala Xaa Ala Xaa Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Xaa Cys Gln Xaa 
            580                 585                 590         
Cys Xaa 
        

<210> 15
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac with mutations that are involved in
      alignment

<220> 
<221> SITE
<222> 325..325
<223> Xaa can be Gly, Ala

<220> 
<221> SITE
<222> 347..347
<223> Xaa can be Asn, Ala, Ser

<220> 
<221> SITE
<222> 350..350
<223> Xaa can be Thr, Ala

<220> 
<221> SITE
<222> 465..465
<223> Xaa can be Trp, Ala

<400> 15
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Arg Gly Arg Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Xaa Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Xaa Trp Phe Xaa Ser Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Xaa Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560 
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Ser Ala Ser Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser 
            580                 585                 590         
Cys Phe 
        

<210> 16
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac with mutations at well conserved
      amino acids

<220> 
<221> SITE
<222> 576..576
<223> Xaa can be lys, Ala

<220> 
<221> SITE
<222> 587..587
<223> Xaa can be Ile, Ala

<400> 16
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Arg Gly Arg Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560 
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Ser Ala Ser Cys Lys Xaa 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Xaa Asp Met Cys Gln Ser 
            580                 585                 590         
Cys Phe 
        

<210> 17
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac with mutations involved in Zn2+
      binding

<220> 
<221> SITE
<222> 586..586
<223> Wherein amino acid can be His, any naturally occuring amino acid

<400> 17
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Arg Gly Arg Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560 
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Ser Ala Ser Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Xaa Ile Asp Met Cys Gln Ser 
            580                 585                 590         
Cys Phe 
        

<210> 18
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac with mutations that are involved in
      integration

<220> 
<221> SITE
<222> 315..315
<223> Wherein amino acid can be Arg, Ala

<220> 
<221> SITE
<222> 341..341
<223> Wherein amino acid can be Arg, Ala

<220> 
<221> SITE
<222> 372..372
<223> Wherein amino acid can be Arg, Ala

<220> 
<221> SITE
<222> 375..375
<223> Wherein amino acid can be Lys, Ala

<400> 18
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Arg Gly Arg Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Xaa Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Xaa Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Xaa Ser Asn Xaa Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560 
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Ser Ala Ser Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser 
            580                 585                 590         
Cys Phe 
        

<210> 19
<211> 897
<212> PRT
<213> Artificial Sequence


<220> 
<223> Cas9 from Corynebacterium ulcerans

<400> 19
Met Thr Asn Ala Val Ala Asn His His Val Leu Trp Ala Lys Phe Asp 
1               5                   10                  15      
Asn Val Ser Glu Pro Tyr Pro Leu Leu Ala His Leu Leu Asp Thr Ala 
            20                  25                  30          
Thr Ala Ala Thr Cys Leu Phe Asn His Trp Leu Arg Lys Gly Leu Arg 
        35                  40                  45              
Asp Arg Leu Ser Thr Glu Leu Gly Pro Asp Ala Glu Lys Ile Leu Gly 
    50                  55                  60                  
Phe Val Ala Gly Ile His Asp Leu Gly Lys Ala Asn Pro Tyr Phe Gln 
65                  70                  75                  80  
Ala Gln Arg Arg Asn Lys Lys Glu Glu Trp Ile Thr Leu Arg Asp Ala 
                85                  90                  95      
Ile Gln Lys Ala Gly Phe Pro Leu Ser Asn Gly Thr Ser Ala Leu Phe 
            100                 105                 110         
Glu Glu Thr Lys Glu Lys Arg Arg His Glu Asn Ile Thr Leu Ser Ile 
        115                 120                 125             
Leu Gly Trp Glu Ile Thr Lys Phe Leu Gln Val Lys Asp Val Trp Pro 
    130                 135                 140                 
Gln Leu Ala Ile Ile Gly His His Gly Asn Phe Ser Ala Pro Gly Phe 
145                 150                 155                 160 
Leu Ser Asp Glu Asp Asp Leu Glu Asp Ile Glu Asp Ile Phe Asp Asp 
                165                 170                 175     
Asn Gly Trp Ser Pro Thr His Glu Leu Leu Val Ser Ser Leu Leu Gln 
            180                 185                 190         
Ala Val Gly Leu Glu Lys Gln Pro Glu Ile Lys His Ile Ser Pro Ala 
        195                 200                 205             
Ser Ala Ile Leu Ile Ser Gly Leu Val Val Leu Ala Asp Arg Ile Ala 
    210                 215                 220                 
Ser Gln Ser Glu Met Ala Ser Asp Gly Leu Gln Ala Leu Gln Lys Glu 
225                 230                 235                 240 
Glu Leu Phe Phe His Gln Pro Glu Lys Trp Ile Ala Asn Arg Lys Ala 
                245                 250                 255     
Phe Cys Arg Glu Ile Ile Glu Asn Thr Val Gly Thr Tyr His Pro Trp 
            260                 265                 270         
Glu Ser Glu Ala Ala Gly Ile Arg Ala Val Leu Gly Asp Tyr Glu Pro 
        275                 280                 285             
Arg Phe Thr Gln Lys Ala Ala Leu Asn Ala Gly Asp Gly Leu Phe Asn 
    290                 295                 300                 
Val Met Glu Thr Thr Gly Ala Gly Lys Thr Glu Ala Ala Leu Leu Arg 
305                 310                 315                 320 
His Val Lys Arg Lys Glu Arg Leu Leu Phe Phe Leu Pro Thr Gln Ala 
                325                 330                 335     
Thr Thr Asn Ala Ile Met Asp Arg Ile Gly Lys Ile Phe Asp Gly Thr 
            340                 345                 350         
Pro Asn Val Ala Ser Leu Ala His Gly Leu Ala Val Thr Glu Asp Phe 
        355                 360                 365             
Tyr Ala His Pro Ile Leu Pro Val Gln Gly Ser Ser Asp Asp Ala Asn 
    370                 375                 380                 
Tyr Lys Asp Asn Gly Gly Leu Tyr Pro Thr Glu Phe Val Arg Ser Ala 
385                 390                 395                 400 
Gly Thr Pro Arg Leu Leu Ala Pro Val Cys Val Gly Thr Ile Asp Gln 
                405                 410                 415     
Ala Leu Met Gly Ala Leu Pro Ser Lys Phe Asn His Leu Arg Leu Leu 
            420                 425                 430         
Ala Leu Ala Asn Ala His Val Val Val Asp Glu Val His Thr Met Asp 
        435                 440                 445             
Gln Tyr Gln Ser Glu Leu Met Ser Gly Leu Leu Glu Trp Trp Ser Ala 
    450                 455                 460                 
Thr Asp Thr Pro Val Thr Leu Leu Thr Ala Thr Met Pro Ala Trp Gln 
465                 470                 475                 480 
Arg Glu Lys Phe His Leu Ser Tyr Thr Gly Lys Asp Pro His Phe Lys 
                485                 490                 495     
Gly Val Phe Pro Ser Leu Glu Asp Trp Ser Thr Pro Ser Lys Asn Thr 
            500                 505                 510         
Glu Thr Ser Gln Glu Asn Ile Pro Thr Glu Ala Phe Thr Ile Pro Ile 
        515                 520                 525             
Asn Ile Asp Lys Ile Ala His Asn Glu Ile Val Asp Ser His Val Gln 
    530                 535                 540                 
Trp Val Ile Glu Gln Arg Lys Leu Phe Pro Gln Ala Arg Ile Gly Ile 
545                 550                 555                 560 
Ile Cys Asn Thr Val Gly Arg Ala Gln Ser Ile Ala Glu Ala Leu Ala 
                565                 570                 575     
His Glu Ser Pro Ile Val Leu His Ser Arg Met Thr Ala Gly His Arg 
            580                 585                 590         
Lys Glu Ala Ala Thr Lys Leu Glu Gln Ala Ile Gly Lys Lys Gly Thr 
        595                 600                 605             
Ala Asn Ala Thr Leu Val Ile Gly Thr Gln Ala Ile Glu Ala Ser Leu 
    610                 615                 620                 
Asp Ile Asp Leu Asp Leu Leu Arg Thr Glu Leu Cys Pro Ala Pro Ser 
625                 630                 635                 640 
Leu Ile Gln Arg Ala Gly Arg Leu Trp Arg Arg Leu Asp Pro Gln Arg 
                645                 650                 655     
Glu Val Arg Val Pro Gly Met Val Gly Lys Lys Leu Thr Ile Ala Val 
            660                 665                 670         
Val Asp Ser Pro Ser Thr Gly Gln Thr Leu Pro Tyr Leu Arg Ser Gln 
        675                 680                 685             
Leu Tyr Arg Val Glu Ser Trp Leu Lys Gln Arg Asp Arg Ile Glu Phe 
    690                 695                 700                 
Pro Ala Asp Ile Gln Asp Phe Ile Asp Ala Thr Thr Pro Gly Leu Gln 
705                 710                 715                 720 
Glu Leu Phe Gln Lys Val Ser Leu Pro Glu Asp Cys Gly Ser Ala Glu 
                725                 730                 735     
Glu Arg Glu Ala Leu Ala Asp Asp Tyr Leu Asn Glu Val Ala Ser Trp 
            740                 745                 750         
Val Thr Lys Gln Arg Gln Ala Gly Thr Ser Arg Ile Asp Phe Ala Lys 
        755                 760                 765             
His Gly Lys Pro Arg Gln Val Leu Ala Ser Asp Cys Val Val Glu Asp 
    770                 775                 780                 
Phe Leu Gln Ile Thr Ser Ala Asn Asn Leu Glu Glu Ser Ala Thr Arg 
785                 790                 795                 800 
Leu Ile Asp Tyr Pro Ser Ile Ser Ala Ile Leu Cys Asp Pro Thr Gly 
                805                 810                 815     
Thr Ile Pro Gly Ala Trp Thr Asp Ser Val Glu Lys Leu Ile Ala Ile 
            820                 825                 830         
Ser Ala Lys Asp Ser Glu Ser Leu Arg Arg Ala Leu Arg Ala Ser Ile 
        835                 840                 845             
Ser Ile Pro His Ser Lys Lys Phe Leu Pro Ile Thr Ser Arg Glu Ile 
    850                 855                 860                 
Pro Leu Ser Glu Ala Lys Thr Leu Leu Ser Gly Tyr Ser Ala Val His 
865                 870                 875                 880 
Ile Gln Pro Asp Glu Tyr Asp Leu Gln Ser Gly Leu Lys Gly Pro Gln 
                885                 890                 895     
Lys 
    

<210> 20
<211> 876
<212> PRT
<213> Artificial Sequence


<220> 
<223> Cas9 from Corynebacterium diphtheria

<400> 20
Met Asn Pro His Glu Glu Leu Trp Ala Lys Gln Lys Gly Leu Ala Lys 
1               5                   10                  15      
Pro Tyr Pro Leu Leu Ala His Leu Leu Asp Ser Ala Ala Val Ala Gly 
            20                  25                  30          
Ala Leu Trp Asp His Trp Leu Arg Gln Asp Leu Arg Gln Met Phe Ile 
        35                  40                  45              
Glu Glu Leu Gly Ser Asn Ala Arg Glu Ile Ile Gln Phe Val Val Gly 
    50                  55                  60                  
Ser His Asp Ile Gly Lys Ala Thr Pro Leu Phe Gln Tyr Gln Lys Ala 
65                  70                  75                  80  
Gln Lys Gly Glu Val Trp Asp Ser Ile Arg Tyr Ala Ile Asp Arg Thr 
                85                  90                  95      
Gly Arg Tyr Gln Lys Pro Leu Pro Ser Ser Tyr Leu Val Lys Lys Thr 
            100                 105                 110         
Ser Gly Gly Pro Asn Arg His Glu Gln Trp Ser Ser Phe Ala Ser Lys 
        115                 120                 125             
Asn Glu Tyr Leu Lys Pro Ser Ala Ala Ala Lys Glu Asn Trp Ile Gly 
    130                 135                 140                 
Leu Ala Ile Gly Gly His His Gly Arg Phe Glu Pro Val Gly Tyr Gly 
145                 150                 155                 160 
Arg His Gln Arg Lys Ala Ala Glu Asp Leu Ala Lys Ser Gly Trp Ser 
                165                 170                 175     
Ala Ala Gln Gln Asp Leu Leu Arg Ala Leu Glu Lys Ala Ser Gly Ile 
            180                 185                 190         
Thr Arg Ala Ser Leu Pro Ser Glu Leu Ser Pro Glu Leu Thr Leu Val 
        195                 200                 205             
Leu Ser Gly Leu Thr Ile Leu Ala Asp Arg Ile Ser Ser Thr Glu Ser 
    210                 215                 220                 
Phe Val Ile Thr Gly Ala Arg Met Ile Asp Asp Gly Thr Leu His Leu 
225                 230                 235                 240 
Ala Thr Pro Ile Asp Trp Leu Lys Thr Arg Lys Leu Asp Ser Glu Lys 
                245                 250                 255     
His Val Ala Lys Thr Val Gly Ile Tyr His Gly Trp Asn Asn His Glu 
            260                 265                 270         
Ser Ala Ile His Ser Ile Leu Lys Gly Tyr Asp Pro Arg Pro Leu Gln 
        275                 280                 285             
Thr Ile Ala Leu Gln Asn Gln Val Gly Leu Leu Asn Leu Met Ala Pro 
    290                 295                 300                 
Thr Gly Asn Gly Lys Thr Glu Ala Ala Ile Leu Arg His Ser Leu Lys 
305                 310                 315                 320 
Glu Asn Asp Arg Leu Ile Phe Leu Leu Pro Thr Gln Ala Thr Ser Asn 
                325                 330                 335     
Ala Ile Met Arg Arg Val Gln Gly Ile Tyr Ser Asp Thr Pro Asn Ala 
            340                 345                 350         
Ala Ala Leu Ala His Ser Leu Ala Ser Val Glu Asp Phe Tyr Gln Thr 
        355                 360                 365             
Pro Leu Ser Val Phe Asp Asp His Tyr Asp Pro Ser Lys Glu Gln Phe 
    370                 375                 380                 
Glu Ser Ser Met Ser Gly Gly Leu Tyr Pro Ser Ser Phe Val Cys Ser 
385                 390                 395                 400 
Gly Ala Ala Arg Leu Leu Ala Pro Ile Cys Ile Gly Thr Val Asp Gln 
                405                 410                 415     
Ala Leu Ala Thr Ala Leu Pro Gly Lys Trp Ile His Leu Arg Ile Leu 
            420                 425                 430         
Ala Leu Ala Asn Ala His Ile Val Ile Asp Glu Val His Thr Leu Asp 
        435                 440                 445             
His Tyr Gln Thr Ala Leu Leu Glu Asn Ile Leu Pro Ile Leu Ala Lys 
    450                 455                 460                 
Leu Lys Thr Lys Ile Thr Phe Leu Thr Ala Thr Met Pro Ser Trp Gln 
465                 470                 475                 480 
Arg Thr Lys Leu Leu Thr Ala Tyr Gly Gly Glu Asp Leu Gln Ile Pro 
                485                 490                 495     
Pro Thr Val Phe Pro Ala Ala Glu Thr Val Leu Pro Gly Gln Phe Asn 
            500                 505                 510         
Arg Thr Leu Ile Asp Ser Asp Ser Thr Thr Ile Asp Phe Thr Met Glu 
        515                 520                 525             
Glu Thr Ser Tyr Asp His Leu Val Glu Ser His Val Lys Trp His Gln 
    530                 535                 540                 
Thr Thr Arg Leu Asn Ala Pro His Ala Arg Ile Gly Leu Ile Cys Asn 
545                 550                 555                 560 
Thr Val Lys Arg Ala Gln Glu Ile Ala Ala Ala Leu Glu Lys Thr Asn 
                565                 570                 575     
Asp Arg Ile Val Leu Leu His Ser Arg Met Thr Thr Glu His Arg Arg 
            580                 585                 590         
Arg Ser Ala Glu Leu Leu Glu Ser Leu Leu Gly Pro Asn Gly Asn Arg 
        595                 600                 605             
Lys Thr Ile Thr Val Val Gly Thr Gln Ala Ile Glu Ala Ser Leu Asp 
    610                 615                 620                 
Ile Asp Leu Asp Ile Leu Arg Thr Glu Leu Cys Pro Ala Pro Ser Leu 
625                 630                 635                 640 
Val Gln Arg Ala Gly Arg Val Trp Arg Arg Asn Asp Pro Tyr Arg Ser 
                645                 650                 655     
Ser Arg Ile Thr Ala Asp His Lys Pro Ile Ser Val Val Phe Ile Ala 
            660                 665                 670         
Glu Ala Lys Asp Trp Gln Val Leu Pro Tyr Leu Arg Ala Glu Thr Ser 
        675                 680                 685             
Arg Thr Gln Arg Trp Leu Glu Lys His Asn Gln Met Phe Leu Pro Gln 
    690                 695                 700                 
Met Ala Gln Glu Phe Ile Asp Ala Ala Thr Val Asp Leu Asp Thr Ala 
705                 710                 715                 720 
Thr Ser Glu Met Asp Leu Asp Ala Leu Ala Leu Met Gly Ile His Leu 
                725                 730                 735     
Met Lys Ala Asp Gly Ala Lys Ala Arg Ile Gln Asp Val Leu Asn Ser 
            740                 745                 750         
Asp Ser Lys Val Ser Asp Phe Ala Leu Leu Thr Ser Lys Asn Glu Ile 
        755                 760                 765             
Asp Glu Ala Gln Thr Arg Leu Ile Glu Glu Gly Thr His Leu Arg Ile 
    770                 775                 780                 
Ile Leu Gly Asp Glu Asn Glu Ser Ile Pro Gly Gly Trp Lys His Gly 
785                 790                 795                 800 
Leu Ser Ser Leu Leu Lys Leu Lys Ala Ser Asp Arg Glu Ser Leu Arg 
                805                 810                 815     
Thr Ala Leu Leu Ala Ser Ile Pro Leu Leu Val Ser Glu Lys Gln Lys 
            820                 825                 830         
Gln Leu Leu Tyr Gln His Asn Leu Val Pro Leu Ser Ser Ser Lys Thr 
        835                 840                 845             
Val Leu Ala Gly Phe Tyr Phe Leu Pro Lys Ala Gln Asn Phe Tyr Ser 
    850                 855                 860                 
Lys Asn Leu Gly Phe Ile Trp Pro Glu Glu Lys Asp 
865                 870                 875     

<210> 21
<211> 773
<212> PRT
<213> Artificial Sequence


<220> 
<223> Cas9 from Spiroplasma syrphidicola

<400> 21
Met Asn Tyr Lys Lys Leu Ile Leu Gly Leu Asp Leu Gly Ile Ala Ser 
1               5                   10                  15      
Cys Gly Trp Ala Val Thr Gly Gln Met Glu Asp Gly Asn Trp Val Leu 
            20                  25                  30          
Asp Asp Phe Gly Val Arg Leu Phe Gln Thr Pro Glu Asn Ser Lys Asp 
        35                  40                  45              
Gly Thr Thr Asn Ala Ala Ala Arg Arg Leu Lys Arg Gly Ala Arg Arg 
    50                  55                  60                  
Leu Ile Lys Arg Arg Lys Asn Arg Ile Lys Asp Leu Lys Asn Leu Phe 
65                  70                  75                  80  
Glu Lys Ile Asn Phe Ile Asn Lys Ala Ser Leu Asp Lys Tyr Ile Asn 
                85                  90                  95      
Glu His Ser Ala Thr Asn Leu Val Glu Asp Phe Asn Arg His Glu Leu 
            100                 105                 110         
Tyr Asn Pro Tyr Phe Leu Arg Ser Ile Gly Ile Thr Glu Lys Leu Thr 
        115                 120                 125             
Arg Glu Glu Leu Val Trp Ser Leu Ile His Ile Ala Asn Arg Arg Gly 
    130                 135                 140                 
Tyr Lys Asn Lys Phe Ala Phe Asp Ile Glu Gly Asp Gly Lys Lys Arg 
145                 150                 155                 160 
Glu Thr Lys Leu Asp Glu Ala Ile Ser Asn Ala Leu Ile Ser Ser Asn 
                165                 170                 175     
Leu Thr Ile Ser Gln Glu Ile Val Arg Asn Lys Lys Phe Arg Asp Ala 
            180                 185                 190         
Lys Asn Lys Lys Ala Leu Leu Val Arg Asn Lys Gly Gly Lys Glu Gly 
        195                 200                 205             
Glu Asn Asn Phe Gln Phe Leu Phe Ala Arg Asp Asp Tyr Lys Lys Glu 
    210                 215                 220                 
Val Asp Leu Leu Leu Ala Lys Gln Ala Lys Phe Tyr Pro Glu Leu Thr 
225                 230                 235                 240 
Glu Glu Ile Arg Ala Lys Ala Ala Asp Ile Ile Phe Arg Gln Arg Asp 
                245                 250                 255     
Phe Glu Asp Gly Pro Gly Pro Lys Lys Gln Glu Leu Arg Glu Ile Tyr 
            260                 265                 270         
Lys Lys Glu Asn Lys Gln Phe Ser Lys Asn Phe Thr Gln Leu Glu Gly 
        275                 280                 285             
Arg Cys Thr Phe Leu Arg Glu Leu Ser Val Gly Tyr Lys Ser Ser Ile 
    290                 295                 300                 
Leu Phe Asp Leu Phe His Ile Ile Ser Glu Val Ser Lys Ile Ser Lys 
305                 310                 315                 320 
Tyr Ile Glu Glu Asn Asp Gln Leu Ala Gln Asp Ile Ile Ser Ser Phe 
                325                 330                 335     
Leu Tyr Asn Glu Ala Gly Lys Lys Gly Lys Thr Leu Leu Lys Glu Ile 
            340                 345                 350         
Leu Lys Lys His His Ile Asn Asp Asp Ile Phe Asp Thr Asn Ala Tyr 
        355                 360                 365             
Lys Asn Ile Asp Phe Lys Thr Asn Tyr Leu Asn Leu Leu Lys Glu Val 
    370                 375                 380                 
Phe Gly Asn Asp Val Leu Lys Asn Leu Ser Leu Asn Arg Leu Glu Asp 
385                 390                 395                 400 
Asn Ile Tyr His Gln Leu Gly Phe Ile Ile His Thr Asn Ile Thr Pro 
                405                 410                 415     
Glu Arg Lys Glu Lys Ala Ile Asn Gln Trp Leu Leu Glu Asn Asn Ile 
            420                 425                 430         
Ile Leu Ala Lys Glu Lys Leu Asn Ile Leu Leu Lys Pro Asn Ser Ser 
        435                 440                 445             
Ile Ser Thr Thr Val Lys Thr Ser Phe Lys Trp Met Ser Ile Ala Ile 
    450                 455                 460                 
Ser Asn Phe Leu Lys Gly Ile Pro Tyr Gly Lys Phe Gln Ala Gln Phe 
465                 470                 475                 480 
Ile Lys Glu Asp Asn Phe Lys Leu Pro Glu Ser Tyr Ala Lys Gln Tyr 
                485                 490                 495     
Gln Lys Tyr Leu Thr Gly Glu Lys Thr Phe Glu Met Phe Ala Pro Ile 
            500                 505                 510         
Ile Asp Pro Asp Leu Trp Arg Asn Pro Ile Val Phe Arg Ala Ile Asn 
        515                 520                 525             
Gln Ala Arg Lys Val Ile Lys Lys Leu Phe Glu Lys Tyr Thr Phe Ile 
    530                 535                 540                 
Asp Gln Ile Asn Ile Glu Leu Thr Arg Glu Met Gly Leu Ser Phe Ser 
545                 550                 555                 560 
Asp Arg Lys Lys Val Lys Glu Arg Gln Asp Asp Ser Leu Lys Glu Asn 
                565                 570                 575     
Ala Lys Ala Lys Glu Phe Leu Met Ala Asn Gly Ile Ile Val Asn Asp 
            580                 585                 590         
Thr Asn Val Leu Lys Tyr Lys Leu Trp Ile Gln Gln Asn Lys Lys Ser 
        595                 600                 605             
Leu Tyr Ser Gly Lys Glu Ile Thr Ile Ala Asp Leu Gly Ala Ser Asn 
    610                 615                 620                 
Val Leu Gln Ile Asp His Ile Ile Pro Tyr Ser Lys Leu Ala Asp Asp 
625                 630                 635                 640 
Ser Phe Asn Asn Lys Val Leu Val Phe Ser Lys Glu Asn Gln Glu Lys 
                645                 650                 655     
Gly Asn Gln Phe Ala Asp Gln Tyr Val Lys Ser Leu Gly Thr Glu Asn 
            660                 665                 670         
Tyr Asn Asn Tyr Lys Lys Arg Val Asn Tyr Leu Leu Phe Gln Asn Gln 
        675                 680                 685             
Ile Asn Gln Lys Lys Ala Glu Tyr Leu Leu Cys Ser Asn Gln Asn Glu 
    690                 695                 700                 
Glu Ile Leu Asn Asp Phe Val Ser Arg Asn Leu Asn Asp Thr Arg Tyr 
705                 710                 715                 720 
Ile Thr Arg Tyr Val Thr Asn Trp Leu Lys Ala Glu Phe Glu Leu Gln 
                725                 730                 735     
Ser Arg Phe Gly Leu Ala Lys Pro Lys Ile Met Thr Leu Asn Gly Ala 
            740                 745                 750         
Ile Thr Ser Arg Phe Arg Arg Thr Trp Leu Arg Asn Ser Pro Trp Gly 
        755                 760                 765             
Leu Glu Lys Lys Ser 
    770             

<210> 22
<211> 1380
<212> PRT
<213> Artificial Sequence


<220> 
<223> Cas9 from Prevotella intermedia

<400> 22
Met Lys Arg Ile Leu Gly Leu Asp Leu Gly Thr Thr Ser Ile Gly Trp 
1               5                   10                  15      
Ala Leu Val Asn Glu Ala Glu Asn Asn Asn Glu Ala Ser Ser Ile Val 
            20                  25                  30          
Arg Leu Gly Val Arg Val Asn Pro Leu Thr Val Asp Glu Lys Ser Asn 
        35                  40                  45              
Phe Glu Lys Gly Lys Ala Ile Thr Thr Asn Ala Asp Arg Gln Leu Arg 
    50                  55                  60                  
His Gly Ala Arg Ile Asn Leu Gln Arg Tyr Lys Leu Arg Arg Gln Asn 
65                  70                  75                  80  
Leu His Asp Cys Leu Gln Lys Gln Gly Trp Leu Gly Thr Glu Ala Met 
                85                  90                  95      
Tyr Glu Glu Gly Lys Ala Ser Thr Phe Glu Thr Tyr Lys Leu Arg Ala 
            100                 105                 110         
Lys Ala Ala Glu Glu Glu Ile Ser Leu His Glu Phe Ala Arg Val Leu 
        115                 120                 125             
Phe Met Leu Asn Lys Lys Arg Gly Tyr Lys Ser Asn Arg Lys Ala Asn 
    130                 135                 140                 
Asn Lys Glu Asp Gly Gln Leu Phe Asp Gly Met Thr Ile Ala Lys Lys 
145                 150                 155                 160 
Leu Tyr Glu Glu His Leu Thr Pro Ala Glu Tyr Ser Leu Gln Leu Leu 
                165                 170                 175     
Asn Lys Gly Lys Lys Phe Thr Gln Gly Tyr Tyr Arg Ser Asp Leu Asn 
            180                 185                 190         
Ala Glu Leu Glu Arg Ile Trp Asp Glu Gln Lys Lys Tyr Tyr Pro Glu 
        195                 200                 205             
Ile Leu Thr Asp Glu Phe Lys Gln Gln Leu Glu Gly Lys Thr Lys Thr 
    210                 215                 220                 
Asn Thr Ser Lys Ile Phe Leu Ala Lys Tyr Gly Ile Tyr Ser Ala Asp 
225                 230                 235                 240 
Leu Lys Gly Leu Asp Arg Lys Phe Gln Pro Leu Lys Trp Arg Val Glu 
                245                 250                 255     
Ala Leu Gln Gln Gln Val Asp Lys Glu Val Leu Ala Phe Val Ile Ser 
            260                 265                 270         
Asp Leu Lys Gly Gln Ile Ala Asn Thr Ser Gly Leu Leu Gly Ala Ile 
        275                 280                 285             
Ser Asp Arg Ser Lys Glu Leu Tyr Phe Asn Lys Gln Thr Val Gly Gln 
    290                 295                 300                 
Tyr Leu Trp Ala Ser Leu Glu Glu Asn Pro His Ile Ser Ile Lys Asn 
305                 310                 315                 320 
Lys Pro Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu Lys Ile Trp 
                325                 330                 335     
Glu Thr Gln Ala Ala Phe His Lys Gln Leu Thr Pro Glu Leu Lys Gln 
            340                 345                 350         
Glu Ile Arg Asp Ile Ile Ile Phe Tyr Gln Arg Pro Leu Lys Ser Lys 
        355                 360                 365             
Lys Ser Leu Ile Ser Val Cys Glu Leu Glu Gln Arg Lys Val Lys Ala 
    370                 375                 380                 
Thr Ile Asp Gly Lys Glu Lys Glu Ile Thr Ile Gly Pro Lys Val Ala 
385                 390                 395                 400 
Pro Lys Ser Ser Pro Val Phe Gln Glu Phe Arg Ile Trp Gln Asn Leu 
                405                 410                 415     
Asn Asn Val Leu Leu Ile Asp Asn Asp Thr Asn Glu Lys Arg Pro Leu 
            420                 425                 430         
Asp Glu Val Glu Arg Asn Leu Leu Tyr Lys Glu Leu Ser Ile Lys Ala 
        435                 440                 445             
Lys Leu Ser Lys Thr Glu Ala Leu Lys Ile Leu Asn Lys Lys Gly Lys 
    450                 455                 460                 
Gln Trp Asp Leu Asn Tyr Arg Glu Leu Glu Gly Asn Arg Thr Gln Ala 
465                 470                 475                 480 
Ile Leu Phe Asp Cys Tyr Asn Arg Ile Ile Thr Leu Thr Gly His Glu 
                485                 490                 495     
Glu Cys Asp Phe Lys Lys Ile Lys Ala Ser Glu Ile Arg His Tyr Val 
            500                 505                 510         
Ser Thr Ile Phe Lys Asn Leu Gly Phe Ser Thr Glu Ile Leu Asp Phe 
        515                 520                 525             
Asp Pro Ser Leu Lys Lys His Glu Leu Glu Lys Gln Pro Met Tyr Gln 
    530                 535                 540                 
Leu Trp His Leu Leu Tyr Ser Tyr Glu Ser Asp Asn Ser Arg Thr Gly 
545                 550                 555                 560 
Asn Glu Ser Leu Leu Arg Lys Leu Glu Thr Thr Phe Gly Phe Pro Glu 
                565                 570                 575     
Glu Tyr Ala Thr Val Leu Cys Asp Val Val Phe Glu Glu Asp Tyr Gly 
            580                 585                 590         
Asn Leu Ser Val Lys Ala Met Arg Glu Ile Leu Pro Tyr Leu Gln Ala 
        595                 600                 605             
Gly Asn Asp Tyr Ser Gln Ala Cys Ala Tyr Ala Gly Tyr Asn His Ser 
    610                 615                 620                 
Arg His Ser Leu Thr Lys Glu Glu Leu Asp Gln Lys Val Tyr Lys Glu 
625                 630                 635                 640 
Arg Leu Glu Leu Leu Pro Lys Asn Ser Leu Arg Asn Pro Val Val Glu 
                645                 650                 655     
Lys Ile Leu Asn Gln Met Ile Asn Val Ile Asn Ala Ile Ile Asp Glu 
            660                 665                 670         
Tyr Gly Lys Pro Asp Glu Ile Arg Ile Glu Met Ala Arg Glu Leu Lys 
        675                 680                 685             
Ser Ser Ala Ala Asp Arg Lys Lys Thr Thr His Ala Ile Ser Gln Gly 
    690                 695                 700                 
Asn Ala Glu Asn Gln Arg Ile Arg Glu Ile Leu Glu Lys Glu Phe Ser 
705                 710                 715                 720 
Leu Ser Tyr Ile Ser Arg Asn Asp Ile Ile Lys Tyr Lys Leu Tyr Glu 
                725                 730                 735     
Glu Leu Glu Pro Asn Tyr Tyr Lys Thr Leu Tyr Ser Asp Thr Tyr Ile 
            740                 745                 750         
Thr Lys Asp Lys Leu Phe Ser Lys Asp Phe Asp Ile Glu His Ile Ile 
        755                 760                 765             
Pro Lys Ala Arg Leu Phe Asp Asp Ser Phe Ser Asn Lys Thr Leu Glu 
    770                 775                 780                 
Ala Arg Asn Ile Asn Leu Glu Lys Ser Asn Lys Thr Ala Phe Asp Phe 
785                 790                 795                 800 
Ile Lys Glu Lys Tyr Gly Glu Asp Gly Ala Glu Ala Tyr Lys Lys Lys 
                805                 810                 815     
Leu Asp Met Leu Leu Glu Asn Asp Ala Ile Ser Arg Pro Lys Tyr Asn 
            820                 825                 830         
Asn Leu Leu Arg Ala Glu Ala Asp Ile Pro Ser Asp Phe Ile Asn Arg 
        835                 840                 845             
Asp Leu Arg Asn Thr Gln Tyr Ile Ala Lys Lys Ala Cys Glu Ile Leu 
    850                 855                 860                 
Gly Glu Leu Val Lys Thr Val Thr Pro Thr Thr Gly Lys Ile Thr Asn 
865                 870                 875                 880 
Arg Leu Arg Glu Asp Trp Gln Leu Val Asp Val Met Lys Glu Leu Asn 
                885                 890                 895     
Phe Glu Lys Tyr Glu Lys Leu Gly Leu Thr Glu Ile Val Glu Asp Arg 
            900                 905                 910         
Asp Gly Arg Lys Ile Lys Arg Ile Lys Asp Trp Thr Lys Arg Asn Asp 
        915                 920                 925             
His Arg His His Ala Met Asp Ala Leu Ala Ile Ala Phe Thr Lys Pro 
    930                 935                 940                 
Ser Phe Ile Gln Tyr Leu Asn Asn Leu Asn Ala Arg Ser Asn Lys Gly 
945                 950                 955                 960 
Asp Ser Ile Tyr Ala Ile Glu Asn Lys Glu Leu His Tyr Glu Glu Gly 
                965                 970                 975     
Lys Leu Arg Phe Asn Ala Pro Ile Pro Val Asn Glu Phe Arg Ala Glu 
            980                 985                 990         
Ala Lys Arg His Leu Ser Ala Ile Leu Val Ser Ile Lys Ala Lys Asn 
        995                 1000                1005            
Lys Val Met Thr Gln Asn Val Asn Lys Ile Lys Thr Lys His Gly Ile 
    1010                1015                1020                
Ile Lys Lys Ile Gln Leu Thr Pro Arg Gly Pro Leu His Asn Glu Thr 
1025                1030                1035                1040
Ile Tyr Gly Thr Lys Met Arg Pro Ile Ile Lys Met Val Lys Val Gly 
                1045                1050                1055    
Ala Ala Leu Asp Glu Ala Thr Ile Asn Lys Val Ser Ser Pro Ala Ile 
            1060                1065                1070        
Arg Glu Ala Leu Leu Lys Arg Leu Asn Glu Tyr Ser Gly Asn Ala Lys 
        1075                1080                1085            
Lys Ala Phe Thr Gly Lys Asn Thr Leu Glu Lys Asn Pro Ile Tyr Leu 
    1090                1095                1100                
Asn Ala Gly Arg Thr Lys Thr Val Pro Ser Leu Val Lys Thr Val Glu 
1105                1110                1115                1120
Trp Glu Ser Phe His Pro Thr Arg Lys Leu Ile Asp Lys Asp Leu Asn 
                1125                1130                1135    
Val Asp Lys Val Val Asp Lys Gly Ile Arg Glu Ile Leu Lys Ala Arg 
            1140                1145                1150        
Leu Glu Glu Phe Asn Gly Asp Ala Lys Lys Ala Phe Ser Asn Leu Glu 
        1155                1160                1165            
Glu Asn Pro Ile Tyr Leu Asp Glu Ala Lys Lys Ile Ala Leu Lys Arg 
    1170                1175                1180                
Val Ser Ile Glu Gly Val Leu Ser Ala Ile Pro Leu His Thr Leu Lys 
1185                1190                1195                1200
Asn Gln Ala Gly Lys Pro Ile Thr Gly Lys Asp Gly Lys Pro Val Leu 
                1205                1210                1215    
Gly Asn Tyr Val Gln Thr Ser Asn Asn His His Ile Ala Phe Tyr Tyr 
            1220                1225                1230        
Asp Glu Asp Gly Asn Leu Gln Asp Asn Ala Val Ser Phe Phe Glu Ala 
        1235                1240                1245            
Ala Glu Arg Lys Ser Gln Gly Ile Pro Val Ile Asp Lys Asp Tyr Asn 
    1250                1255                1260                
Arg Asp Lys Gly Trp Arg Phe Leu Phe Thr Met Lys Gln Asn Glu Tyr 
1265                1270                1275                1280
Phe Val Phe Pro Asn Glu Ala Thr Gly Phe Ile Pro Ser Glu Val Asp 
                1285                1290                1295    
Leu Thr Asp Glu Ala Asn Tyr Gly Ile Ile Ser Pro Asn Leu Tyr Arg 
            1300                1305                1310        
Val Gln Lys Val Ser Arg Ile Asp Lys Gly Thr Ser Ala Ser Arg Asp 
        1315                1320                1325            
Tyr Trp Phe Arg His His Leu Glu Thr Ile Leu Asn Asp Asp Ala Lys 
    1330                1335                1340                
Leu Lys Asn Leu Ala Phe Lys Arg Ile Arg Gly Leu Leu Glu Leu Lys 
1345                1350                1355                1360
Asp Ile Ile Lys Val Arg Ile Asn Ser Thr Gly Lys Ile Val Ala Val 
                1365                1370                1375    
Gly Glu Tyr Asp 
            1380

<210> 23
<211> 535
<212> PRT
<213> Artificial Sequence


<220> 
<223> Cas9 from Spiroplasma taiwanense

<400> 23
Met Trp Ser Arg Lys Ile Leu Lys Ala Gly Ser Arg Leu Phe Asp Glu 
1               5                   10                  15      
Ala Asn Leu Ser Asp Lys Ile Ala Ser Lys Arg Arg Glu Gln Arg Gly 
            20                  25                  30          
Arg Arg Arg Asn Leu Arg Arg Lys Ile Thr Trp Lys Gln Asp Leu Ile 
        35                  40                  45              
Asn Leu Phe Val Lys Tyr Asn Phe Leu Gln Lys Glu Asn Asp Phe Tyr 
    50                  55                  60                  
Glu Leu Asp Phe Asn Phe Asp Leu Leu Glu Leu Arg Lys Lys Ala Ile 
65                  70                  75                  80  
Asn Ser Lys Ile Glu Leu Glu Gln Leu Leu Ile Ile Leu Phe Asn Tyr 
                85                  90                  95      
Ile Lys His Arg Gly Ser Phe Asn Tyr Arg Glu Asp Leu Ser Glu Leu 
            100                 105                 110         
Lys Asn Ile Ser Gln Glu Glu Leu Glu Thr Ser Ser Glu Phe Lys Leu 
        115                 120                 125             
Pro Val Asp Ile Gln Phe Glu Leu Lys Glu Glu Asn Asn Lys Phe Arg 
    130                 135                 140                 
Glu Ile Asn Asn Glu Lys Ser Leu Ile Asn His Glu Trp Tyr Val Lys 
145                 150                 155                 160 
Glu Ile Asn Leu Ile Leu Asp Ala Gln Ile Glu Asn Lys Leu Ile Asn 
                165                 170                 175     
Leu Asp Phe Lys Lys Asp Tyr Leu Lys Leu Phe Asn Arg Lys Arg Glu 
            180                 185                 190         
Tyr Tyr Asp Gly Pro Gly Pro Lys Asp Lys Asn Leu Leu Asn Pro Ser 
        195                 200                 205             
Lys Tyr Gly Trp Lys Asn Gln Glu Glu Phe Phe Asp Arg Phe Ala Gly 
    210                 215                 220                 
Lys Asp Thr Tyr Asp Ser Lys Glu Gln Arg Ala Pro Lys His Ser Leu 
225                 230                 235                 240 
Thr Ser Tyr Leu Phe Asn Ile Leu Asn Asp Leu Asn Asn Leu Ser Ile 
                245                 250                 255     
Asn Gly Asp Arg Asn Gln Leu Thr Tyr Glu Asn Lys Lys Asp Leu Ile 
            260                 265                 270         
Asn Leu Thr Leu Ile Asn Gln Lys Glu Lys Ala Glu Asn Ile Thr Leu 
        275                 280                 285             
Lys Lys Ile Ala Lys Tyr Leu Lys Ile Asn Glu Lys Asn Ile Thr Gly 
    290                 295                 300                 
Tyr Arg Leu Lys Pro Asn Ser Asn Glu Ser Ile Phe Thr Val Phe Glu 
305                 310                 315                 320 
Ser Ala Asn Lys Met Arg Ser Ile Leu Val Lys Asn Asn Lys Ser Ile 
                325                 330                 335     
Asp Phe Ile Cys Leu Glu Asn Ile Asp Lys Ile Asp Lys Ile Val Asp 
            340                 345                 350         
Ile Leu Thr Lys Tyr Gln Ser Ile Glu Asp Lys Ser Leu Lys Leu Glu 
        355                 360                 365             
Glu Leu Asn Phe Asp Phe Phe Asp Lys Glu Thr Cys Glu Lys Leu Ala 
    370                 375                 380                 
Val Ile Ser Leu Thr Gly Thr His Ala Leu Ser Lys Lys Thr Met Ser 
385                 390                 395                 400 
Lys Leu Ile Glu Glu Met Phe His Asp Asn Leu Asn His Met Glu Ala 
                405                 410                 415     
Leu Ala Lys Leu Lys Ile Lys Pro Asp Tyr Lys Leu Lys Val Asp Leu 
            420                 425                 430         
Thr Asn Phe Lys Thr Ile Pro Ile Leu Arg Glu Lys Ile Asn Glu Met 
        435                 440                 445             
Tyr Ile Ser Pro Val Val Lys Arg Ala Leu Ile Glu Ser Leu Lys Ile 
    450                 455                 460                 
Ile Lys Glu Leu Glu Arg His Phe Lys Asp Phe Glu Ile Lys Asp Ile 
465                 470                 475                 480 
Val Ile Glu Met Ala Lys Lys Asn Ser Ala Glu Lys Lys Gln Phe Ile 
                485                 490                 495     
Ser Lys Ile Gln Arg Gln Asn Val Asp Leu Val Lys Lys Leu Ser Asn 
            500                 505                 510         
Asp Tyr Ser Leu Asp Glu Asn Lys Leu Asn Phe Lys Met Lys Glu Lys 
        515                 520                 525             
Phe Leu Leu Leu Ser Glu Gln 
    530                 535 

<210> 24
<211> 1281
<212> PRT
<213> Artificial Sequence


<220> 
<223> Cas9 from Streptococcus iniae

<400> 24
Met Arg Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          
Arg Ile Gln Gly Thr Thr Asp Arg Thr Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Asn Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Thr Arg Arg Arg Tyr Thr Arg Arg Lys Tyr Arg Ile Lys 
65                  70                  75                  80  
Glu Leu Gln Lys Ile Phe Ser Ser Glu Met Asn Glu Leu Asp Ile Ala 
                85                  90                  95      
Phe Phe Pro Arg Leu Ser Glu Ser Phe Leu Val Ser Asp Asp Lys Glu 
            100                 105                 110         
Phe Glu Asn His Pro Ile Phe Gly Asn Leu Lys Asp Glu Ile Thr Tyr 
        115                 120                 125             
His Asn Asp Tyr Pro Thr Ile Tyr His Leu Arg Gln Thr Leu Ala Asp 
    130                 135                 140                 
Ser Asp Gln Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asn Leu Asp Ser 
                165                 170                 175     
Glu Asn Thr Asp Val His Val Leu Phe Leu Asn Leu Val Asn Ile Tyr 
            180                 185                 190         
Asn Asn Leu Phe Glu Glu Asp Ile Val Glu Thr Ala Ser Ile Asp Ala 
        195                 200                 205             
Glu Lys Ile Leu Thr Ser Lys Thr Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ala Glu Ile Pro Asn Gln Lys Arg Asn Met Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Val Ser Leu Ala Leu Gly Leu Thr Pro Asn Phe Lys Thr Asn Phe 
                245                 250                 255     
Glu Leu Leu Glu Asp Ala Lys Leu Gln Ile Ser Lys Asp Ser Tyr Glu 
            260                 265                 270         
Glu Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Ile Ala Ala Lys Lys Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Ile Thr Val Lys Gly Ala Ser Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Val Gln Arg Tyr Glu Glu His Gln Gln Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Asn Leu Val Lys Lys Gln Ile Pro Glu Lys Tyr Lys Glu Ile Phe Asp 
            340                 345                 350         
Asn Lys Glu Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Ser 
        355                 360                 365             
Gln Glu Glu Phe Tyr Lys Tyr Ile Lys Pro Ile Leu Leu Lys Leu Asp 
    370                 375                 380                 
Gly Thr Glu Lys Leu Ile Ser Lys Leu Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Asn Glu Leu Lys Ala Ile Ile Arg Arg Gln Glu Lys Phe Tyr Pro Phe 
            420                 425                 430         
Leu Lys Glu Asn Gln Lys Lys Ile Glu Lys Leu Phe Thr Phe Lys Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Asn Gly Gln Ser Ser Phe Ala Trp 
    450                 455                 460                 
Leu Lys Arg Gln Ser Asn Glu Ser Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Gln Glu Ala Ser Ala Arg Ala Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Thr Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Pro Leu Tyr Glu Met Phe Met Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Gln Thr Glu Gly Met Lys Arg Pro Val Phe Leu Ser Ser Glu Asp 
    530                 535                 540                 
Lys Glu Glu Ile Val Asn Leu Leu Phe Lys Lys Glu Arg Lys Val Thr 
545                 550                 555                 560 
Val Lys Gln Leu Lys Glu Glu Tyr Phe Ser Lys Met Lys Cys Phe His 
                565                 570                 575     
Thr Val Thr Ile Leu Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Phe Lys Asp Lys Ala Phe Leu Asp 
        595                 600                 605             
Asp Glu Ala Asn Gln Asp Ile Leu Glu Glu Ile Val Trp Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Gln Ala Met Ile Glu Arg Arg Leu Val Lys Tyr Ala 
625                 630                 635                 640 
Asp Val Phe Glu Lys Ser Val Leu Lys Lys Leu Lys Lys Arg His Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Gln Lys Leu Ile Asn Gly Ile Lys Asp 
            660                 665                 670         
Lys Gln Thr Gly Lys Thr Ile Leu Gly Phe Leu Lys Asp Asp Gly Val 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Ser Ser Leu Asp Phe 
    690                 695                 700                 
Ala Lys Ile Ile Lys Asn Glu Gln Glu Lys Thr Ile Lys Asn Glu Ser 
705                 710                 715                 720 
Leu Glu Glu Thr Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys 
                725                 730                 735     
Gly Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Ile Val Lys Ile Met 
            740                 745                 750         
Gly Gln Asn Pro Asp Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Ser Thr Met Gln Gly Ile Lys Asn Ser Arg Gln Arg Leu Arg Lys Leu 
    770                 775                 780                 
Glu Glu Val His Lys Asn Thr Gly Ser Lys Ile Leu Lys Glu Tyr Asn 
785                 790                 795                 800 
Val Ser Asn Thr Gln Leu Gln Ser Asp Arg Leu Tyr Leu Tyr Leu Leu 
                805                 810                 815     
Gln Asp Gly Lys Asp Met Tyr Thr Gly Lys Glu Leu Asp Tyr Asp Asn 
            820                 825                 830         
Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ser Phe Ile Lys 
        835                 840                 845             
Asp Asn Ser Ile Asp Asn Thr Val Leu Thr Thr Gln Ala Ser Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Asn Ile Glu Thr Val Asn Lys Met Lys 
865                 870                 875                 880 
Ser Phe Trp Tyr Lys Gln Leu Lys Ser Gly Ala Ile Ser Gln Arg Lys 
                885                 890                 895     
Phe Asp His Leu Thr Lys Ala Glu Arg Gly Ala Leu Ser Asp Phe Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ser Arg Phe Asn Ser Asn Leu Thr 
    930                 935                 940                 
Glu Asp Ser Lys Ser Asn Arg Asn Val Lys Ile Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Lys Met Val Ser Asp Phe Arg Lys Asp Phe Gly Phe Tyr Lys Leu Arg 
                965                 970                 975     
Glu Val Asn Asp Tyr His His Ala Gln Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Leu Lys Lys Tyr Pro Lys Leu Glu Ala Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Lys His Tyr Asp Leu Ala Lys Leu Met Ile Gln 
    1010                1015                1020                
Pro Asp Ser Ser Leu Gly Lys Ala Thr Thr Arg Met Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Leu Met Asn Phe Phe Lys Lys Glu Ile Lys Leu Ala Asp Asp Thr 
                1045                1050                1055    
Ile Phe Thr Arg Pro Gln Ile Glu Val Asn Thr Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Val Lys Asp Met Gln Thr Ile Arg Lys Val Met Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Ile Val Met Lys Thr Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Trp Pro Lys Gly Asp Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Ala Arg Lys Lys Ser Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Ile Ile Ala Tyr Ser Val Leu Val Val Ala Lys Ile Ala Lys Gly 
            1140                1145                1150        
Lys Thr Gln Lys Leu Lys Thr Ile Lys Glu Leu Val Gly Ile Lys Ile 
        1155                1160                1165            
Met Glu Gln Asp Glu Phe Glu Lys Asp Pro Ile Ala Phe Leu Glu Lys 
    1170                1175                1180                
Lys Gly Tyr Gln Asp Ile Gln Thr Ser Ser Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg Leu Leu Ala Ser 
                1205                1210                1215    
Ala Lys Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu Pro Asn Lys Tyr 
            1220                1225                1230        
Val Lys Phe Leu Tyr Leu Ala Ser His Tyr Thr Lys Phe Thr Gly Lys 
        1235                1240                1245            
Glu Glu Asp Arg Glu Lys Lys Arg Ser Tyr Val Glu Ser His Leu Tyr 
    1250                1255                1260                
Tyr Phe Asp Val Arg Leu Ser Gln Val Phe Arg Val Thr Asn Val Glu 
1265                1270                1275                1280
Phe 
    

<210> 25
<211> 1352
<212> PRT
<213> Artificial Sequence


<220> 
<223> Cas9 from Belliella baltica

<400> 25
Met Lys Lys Ile Leu Gly Leu Asp Leu Gly Thr Thr Ser Ile Gly Trp 
1               5                   10                  15      
Ala Phe Ile Lys Glu Pro Glu Lys Asp Val Val Gly Ser Glu Ile Val 
            20                  25                  30          
Asp Met Gly Val Arg Ile Val Pro Leu Ser Ser Asp Glu Glu Asn Asp 
        35                  40                  45              
Phe Ala Lys Gly Asn Thr Ile Ser Ile Asn Ala Asp Arg Thr Leu Lys 
    50                  55                  60                  
Arg Gly Ala Arg Arg Asn Leu Gln Arg Phe Lys Gln Arg Arg Asn Ala 
65                  70                  75                  80  
Leu Leu Glu Ile Phe Lys Glu Lys Lys Leu Ile Ser Thr Asn Phe Lys 
                85                  90                  95      
Tyr Ala Glu Asp Gly Pro Ser Ser Thr Phe Ser Thr Leu Asn Leu Arg 
            100                 105                 110         
Ala Lys Ala Ala Lys Glu Lys Ile Glu Leu Gln Asp Leu Val Lys Val 
        115                 120                 125             
Leu Leu Gln Ile Asn Lys Lys Arg Gly Tyr Lys Ser Ser Arg Lys Ala 
    130                 135                 140                 
Lys Ser Glu Glu Asp Asp Gly Ser Ala Ile Asp Ser Met Gly Ile Ala 
145                 150                 155                 160 
Lys Glu Leu Tyr Glu Asn Asp Leu Thr Pro Gly Gln Trp Val Tyr Glu 
                165                 170                 175     
Ala Leu Gln Lys Gly Arg Lys Asn Val Pro Asp Phe Tyr Arg Ser Asp 
            180                 185                 190         
Leu Gln Glu Glu Phe Lys Lys Ile Val Asn Tyr Gln Ser Glu Phe Phe 
        195                 200                 205             
Pro Asp Ile Phe Asn Ala Ser Phe Val Glu Asp Trp Met Gly Lys Ala 
    210                 215                 220                 
Ser Thr Pro Thr Lys Gln Tyr Phe Asn Lys Lys Gly Val Gln Leu Ala 
225                 230                 235                 240 
Glu Asn Lys Gly Lys Arg Glu Glu Arg Arg Leu Gln Glu Tyr Lys Trp 
                245                 250                 255     
Arg Ala Glu Ala Val Asn Phe Lys Ile Asp Leu Ser Glu Ile Ala Leu 
            260                 265                 270         
Ile Leu Ser Gln Ile Asn Ser Gln Ile Ser Asn Ser Ser Gly Tyr Leu 
        275                 280                 285             
Gly Ala Ile Ser Asp Arg Ser Lys Glu Leu Tyr Phe Lys Asn Leu Thr 
    290                 295                 300                 
Val Gly Gln Tyr Leu Tyr Gln Gln Ile Lys Lys Asn Pro His Thr Arg 
305                 310                 315                 320 
Leu Lys Gly Gln Val Phe Tyr Arg Gln Asp Tyr Leu Asp Glu Phe Glu 
                325                 330                 335     
Arg Ile Trp Ser Val Gln Ser Ser Phe Tyr Pro Gln Leu Asn Asp Ala 
            340                 345                 350         
Leu Lys Arg Glu Val Arg Asp Ile Thr Ile Phe Phe Gln Arg Arg Leu 
        355                 360                 365             
Lys Ser Gln Lys His Leu Ile Ser Asn Cys Glu Phe Glu Asp His His 
    370                 375                 380                 
Lys Val Val Pro Lys Ser His Pro Val Phe Gln Glu Phe Arg Ile Trp 
385                 390                 395                 400 
Gln Asn Leu Asn Asn Leu Leu Leu Ile Lys Lys Asp Asn Leu Asn Glu 
                405                 410                 415     
Lys Phe Asp Leu Glu Leu Glu Ser Lys Ile Ala Leu Ala Asn Glu Leu 
            420                 425                 430         
Ala Phe Lys Arg Glu Leu Asn Val Lys Asp Ala Leu Lys Ile Leu Gly 
        435                 440                 445             
Leu Lys Pro Asn Glu Trp Glu Phe Asn Phe Thr Lys Ile Glu Gly Asn 
    450                 455                 460                 
Arg Thr Asn Gln Ala Phe Phe Asp Ala Phe Ala Lys Ile Ile Glu Leu 
465                 470                 475                 480 
Glu Asp Gly Glu Pro Ile Asp Leu Gly Asp Leu Lys Ala Asp Asp Ile 
                485                 490                 495     
Leu Asp Gln Phe Ser Glu Ala Phe Leu Arg Ile Gly Ile Asp Thr Glu 
            500                 505                 510         
Leu Leu Gln Val Asn Ser Asp Ile Glu Gly Ala Glu Tyr Glu Lys Gln 
        515                 520                 525             
Ser Tyr Ile Gln Phe Trp His Leu Leu Tyr Ser Ser Glu Asp Asp Gln 
    530                 535                 540                 
Lys Leu Lys Leu Asn Leu Ile Arg Lys Phe Gly Phe Lys Pro Glu His 
545                 550                 555                 560 
Ala Lys Ile Leu Ala Ser Ile Ser Leu Gln Asp Asp His Ala Ser Leu 
                565                 570                 575     
Ser Ser Arg Ala Ile Lys Lys Ile Leu Pro His Leu Gln Ser Gly Leu 
            580                 585                 590         
Ile Tyr Asp Lys Ala Cys Thr Tyr Ala Gly Tyr Asn His Ser Ser Ser 
        595                 600                 605             
Phe Thr Lys Asp Glu Asn Glu Lys Arg Glu Leu Arg Ala Glu Leu Glu 
    610                 615                 620                 
Leu Leu Lys Lys Asn Ser Leu Arg Asn Pro Val Val Glu Lys Ile Leu 
625                 630                 635                 640 
Asn Gln Met Ile Asn Val Val Asn Ala Ile Leu Lys Asp Pro Glu Leu 
                645                 650                 655     
Gly Arg Pro Asp Glu Ile Arg Val Glu Met Ala Arg Glu Leu Lys Ala 
            660                 665                 670         
Asn Ala Glu Gln Arg Lys Asn Met Thr Ser Asn Ile Ala Ser Ala Thr 
        675                 680                 685             
Arg Asp His Asp Lys Tyr Arg Glu Ile Leu Lys Ser Glu Phe Gly Leu 
    690                 695                 700                 
Lys Arg Val Thr Lys Asn Asp Leu Leu Arg Tyr Lys Leu Trp Leu Glu 
705                 710                 715                 720 
Thr Asp Gly Ile Ser Leu Tyr Thr Gly Lys Pro Ile Glu Ala Ser Lys 
                725                 730                 735     
Leu Phe Ser Lys Glu Tyr Asp Ile Glu His Ile Ile Pro Lys Ala Arg 
            740                 745                 750         
Leu Phe Asp Asp Ser Phe Ser Asn Lys Thr Ile Cys Glu Arg Gln Leu 
        755                 760                 765             
Asn Ile Asp Lys Ala Asn Val Thr Ala Phe Ser Phe Leu Gln Asn Lys 
    770                 775                 780                 
Leu Ser Ala Asp Glu Phe Glu Gln Tyr Gln Ser Arg Val Lys Ser Leu 
785                 790                 795                 800 
Tyr Gly Lys Leu Ser Lys Ala Lys Ile Gln Lys Leu Leu Met Ala Asn 
                805                 810                 815     
Asp Lys Ile Pro Glu Asp Phe Ile Ala Arg Gln Leu Gln Glu Thr Arg 
            820                 825                 830         
Tyr Ile Ser Lys Lys Ala Lys Glu Ile Leu Phe Glu Ile Ser Arg Arg 
        835                 840                 845             
Val Ser Val Thr Thr Gly Thr Ile Thr Asp Lys Leu Arg Glu Asp Trp 
    850                 855                 860                 
Gly Leu Val Glu Ile Met Lys Glu Leu Asn Trp Glu Lys Tyr Asp Lys 
865                 870                 875                 880 
Leu Gly Leu Thr Tyr Thr Ile Glu Gly Lys His Gly Glu Arg Leu Asn 
                885                 890                 895     
Lys Ile Lys Asp Trp Ser Lys Arg Asn Asp His Arg His His Ala Met 
            900                 905                 910         
Asp Ala Leu Thr Val Ala Leu Thr Lys Pro Ala Tyr Ile Gln Tyr Leu 
        915                 920                 925             
Asn Asn Leu Asn Ala Lys Gly Leu Asn Asn Lys Lys Gly Thr Glu Val 
    930                 935                 940                 
Phe Ala Ile Glu Gln Lys Tyr Leu Lys Arg Glu Asn Gly Lys Leu Cys 
945                 950                 955                 960 
Phe Ile Pro Pro Ile Glu Asn Ile Arg Ser Glu Ala Lys Lys His Leu 
                965                 970                 975     
Ser Arg Ile Leu Val Ser Tyr Lys Ala Lys Asn Lys Val Val Thr Ile 
            980                 985                 990         
Asn Lys Asn Lys Thr Lys Ser Lys Ala Gly Leu Asn Glu Gln Ile Ala 
        995                 1000                1005            
Leu Thr Pro Arg Gly Gln Leu His Lys Glu Thr Val Tyr Gly Lys Ser 
    1010                1015                1020                
Phe His Tyr Ser Thr Lys Phe Glu Lys Ile Gly Ala Ser Phe Asn Val 
1025                1030                1035                1040
Gln Lys Ile Asn Thr Val Ala Lys Lys Glu Glu Arg Glu Ala Leu Leu 
                1045                1050                1055    
Lys Arg Leu Ala Glu Asn Gly Asn Asp Pro Lys Lys Ala Phe Thr Gly 
            1060                1065                1070        
Lys Asn Thr Leu Asn Lys Met Pro Ile Tyr Leu Asp Leu Gly Lys Asn 
        1075                1080                1085            
Ile Lys Leu Ser Glu Lys Val Lys Thr Val Val Leu Glu Gln Asn Tyr 
    1090                1095                1100                
Thr Ile Arg Lys Asn Ile Asp Pro Asp Leu Lys Val Asp Lys Val Ile 
1105                1110                1115                1120
Asp Val Gly Ile Lys Arg Ile Leu Glu Ser Arg Leu Glu Glu Phe Gly 
                1125                1130                1135    
Gly Asn Ala Lys Leu Ala Phe Ser Asn Leu Glu Glu Asn Pro Ile Trp 
            1140                1145                1150        
Leu Asn Lys Glu Lys Gly Ile Ser Ile Lys Arg Val Lys Ile Ser Gly 
        1155                1160                1165            
Val Ser Asn Val Glu Ser Leu His Val Lys Lys Asp His Phe Gly Glu 
    1170                1175                1180                
Pro Ile Leu Asp Gln Glu Gly Asn Glu Ile Pro Val Asp Phe Val Ser 
1185                1190                1195                1200
Thr Gly Asn Asn His His Val Ala Ile Tyr Glu Asp Glu Asn Gly Asn 
                1205                1210                1215    
Leu Gln Glu Glu Val Val Ser Phe Phe Glu Ala Val Val Arg Gln Asn 
            1220                1225                1230        
Gln Gly Leu Pro Ile Ile Lys Lys Asn His Thr Leu Gly Trp Lys Phe 
        1235                1240                1245            
Leu Phe Thr Leu Lys Gln Asn Glu Tyr Phe Val Phe Pro Ser Asp Asp 
    1250                1255                1260                
Phe Val Pro Ala Asp Val Asp Leu Met Asp Glu Gln Asn Tyr His Leu 
1265                1270                1275                1280
Ile Ser Pro Asn Leu Phe Arg Val Gln Lys Ile Ala Arg Lys Asn Tyr 
                1285                1290                1295    
Val Phe Asn Asn His Leu Glu Thr Lys Ala Val Asp Asn Asp Leu Leu 
            1300                1305                1310        
Lys Ser Lys Lys Glu Leu Ser Lys Ile Thr Tyr His Phe Tyr Gln Thr 
        1315                1320                1325            
Pro Glu His Leu Arg Gly Ile Ile Lys Ile Arg Ile Asn His Leu Gly 
    1330                1335                1340                
Lys Ile Ile Gln Ile Gly Glu Tyr 
1345                1350        

<210> 26
<211> 1509
<212> PRT
<213> Artificial Sequence


<220> 
<223> Cas9 from Psychroflexus torquisI

<400> 26
Met Lys Arg Ile Leu Gly Leu Asp Leu Gly Thr Asn Ser Ile Gly Trp 
1               5                   10                  15      
Ser Leu Ile Glu His Asp Phe Lys Asn Lys Gln Gly Gln Ile Glu Gly 
            20                  25                  30          
Leu Gly Val Arg Ile Ile Pro Met Ser Gln Glu Ile Leu Gly Lys Phe 
        35                  40                  45              
Asp Ala Gly Gln Ser Ile Ser Gln Thr Ala Asp Arg Thr Lys Tyr Arg 
    50                  55                  60                  
Gly Val Arg Arg Leu Tyr Gln Arg Asp Asn Leu Arg Arg Glu Arg Leu 
65                  70                  75                  80  
His Arg Val Leu Lys Ile Leu Asp Phe Leu Pro Lys His Tyr Ser Glu 
                85                  90                  95      
Ser Ile Asp Phe Gln Asp Lys Val Gly Gln Phe Lys Pro Lys Gln Glu 
            100                 105                 110         
Val Lys Leu Asn Tyr Arg Lys Asn Glu Lys Asn Lys His Glu Phe Val 
        115                 120                 125             
Phe Met Asn Ser Phe Ile Glu Met Val Ser Glu Phe Lys Asn Ala Gln 
    130                 135                 140                 
Pro Glu Leu Phe Tyr Asn Lys Gly Asn Gly Glu Glu Thr Lys Ile Pro 
145                 150                 155                 160 
Tyr Asp Trp Thr Leu Tyr Tyr Leu Arg Lys Lys Ala Leu Thr Gln Gln 
                165                 170                 175     
Ile Thr Lys Glu Glu Leu Ala Trp Leu Ile Leu Asn Phe Asn Gln Lys 
            180                 185                 190         
Arg Gly Tyr Tyr Gln Leu Arg Gly Glu Asp Ile Asp Glu Asp Lys Asn 
        195                 200                 205             
Lys Lys Tyr Met Gln Leu Lys Val Asn Asn Leu Ile Asp Ser Gly Ala 
    210                 215                 220                 
Lys Val Lys Gly Lys Val Leu Tyr Asn Val Ile Phe Asp Asn Gly Trp 
225                 230                 235                 240 
Lys Tyr Glu Lys Gln Ile Val Asn Lys Asp Glu Trp Glu Gly Arg Thr 
                245                 250                 255     
Lys Glu Phe Ile Ile Thr Thr Lys Thr Leu Lys Asn Gly Asn Ile Lys 
            260                 265                 270         
Arg Thr Tyr Lys Ala Val Asp Ser Glu Ile Asp Trp Ala Ala Ile Lys 
        275                 280                 285             
Ala Lys Thr Glu Gln Asp Ile Asn Lys Ala Asn Lys Thr Val Gly Glu 
    290                 295                 300                 
Tyr Ile Tyr Glu Ser Leu Leu Asp Asn Pro Ser Gln Lys Ile Arg Gly 
305                 310                 315                 320 
Lys Leu Val Lys Thr Ile Glu Arg Lys Phe Tyr Lys Glu Glu Phe Glu 
                325                 330                 335     
Lys Leu Leu Ser Lys Gln Ile Glu Leu Gln Pro Glu Leu Phe Asn Glu 
            340                 345                 350         
Ser Leu Tyr Lys Ala Cys Ile Lys Glu Leu Tyr Pro Arg Asn Glu Asn 
        355                 360                 365             
His Gln Ser Asn Asn Lys Lys Gln Gly Phe Glu Tyr Leu Phe Thr Glu 
    370                 375                 380                 
Asp Ile Ile Phe Tyr Gln Arg Pro Leu Lys Ser Gln Lys Ser Asn Ile 
385                 390                 395                 400 
Ser Gly Cys Gln Phe Glu His Lys Ile Tyr Lys Gln Lys Asn Lys Lys 
                405                 410                 415     
Thr Gly Lys Leu Glu Leu Ile Lys Glu Pro Ile Lys Thr Ile Ser Arg 
            420                 425                 430         
Ser His Pro Leu Phe Gln Glu Phe Arg Ile Trp Gln Trp Leu Gln Asn 
        435                 440                 445             
Leu Lys Ile Tyr Asn Lys Glu Lys Ile Glu Asn Gly Lys Leu Glu Asp 
    450                 455                 460                 
Val Thr Thr Gln Leu Leu Pro Asn Asn Glu Ala Tyr Val Thr Leu Phe 
465                 470                 475                 480 
Asp Phe Leu Asn Thr Lys Lys Glu Leu Glu Gln Lys Gln Phe Ile Glu 
                485                 490                 495     
Tyr Phe Val Lys Lys Lys Leu Ile Asp Lys Lys Glu Lys Glu His Phe 
            500                 505                 510         
Arg Trp Asn Phe Val Glu Asp Lys Lys Tyr Pro Phe Ser Glu Thr Arg 
        515                 520                 525             
Ala Gln Phe Leu Ser Arg Leu Ala Lys Val Lys Gly Ile Lys Asn Thr 
    530                 535                 540                 
Glu Asp Phe Leu Asn Lys Asn Thr Gln Val Gly Ser Lys Glu Asn Ser 
545                 550                 555                 560 
Pro Phe Ile Lys Arg Ile Glu Gln Leu Trp His Ile Ile Tyr Ser Val 
                565                 570                 575     
Ser Asp Leu Lys Glu Tyr Glu Lys Ala Leu Glu Lys Phe Ala Glu Lys 
            580                 585                 590         
His Asn Leu Glu Lys Asp Ser Phe Leu Lys Asn Phe Lys Lys Phe Pro 
        595                 600                 605             
Pro Phe Val Ser Asp Tyr Ala Ser Tyr Ser Lys Lys Ala Ile Ser Lys 
    610                 615                 620                 
Leu Leu Pro Ile Met Arg Met Gly Lys Tyr Trp Ser Glu Ser Ala Val 
625                 630                 635                 640 
Pro Thr Gln Val Lys Glu Arg Ser Leu Ser Ile Met Glu Arg Val Lys 
                645                 650                 655     
Val Leu Pro Leu Lys Glu Gly Tyr Ser Asp Lys Asp Leu Ala Asp Leu 
            660                 665                 670         
Leu Ser Arg Val Ser Asp Asp Asp Ile Pro Lys Gln Leu Ile Lys Ser 
        675                 680                 685             
Phe Ile Ser Phe Lys Asp Lys Asn Pro Leu Lys Gly Leu Asn Thr Tyr 
    690                 695                 700                 
Gln Ala Asn Tyr Leu Val Tyr Gly Arg His Ser Glu Thr Gly Asp Ile 
705                 710                 715                 720 
Gln His Trp Lys Thr Pro Glu Asp Ile Asp Arg Tyr Leu Asn Asn Phe 
                725                 730                 735     
Lys Gln His Ser Leu Arg Asn Pro Ile Val Glu Gln Val Val Met Glu 
            740                 745                 750         
Thr Leu Arg Val Val Arg Asp Ile Trp Glu His Tyr Gly Asn Asn Glu 
        755                 760                 765             
Lys Asp Phe Phe Lys Glu Ile His Val Glu Leu Gly Arg Glu Met Lys 
    770                 775                 780                 
Ser Pro Ala Gly Lys Arg Glu Lys Leu Ser Gln Arg Asn Thr Glu Asn 
785                 790                 795                 800 
Glu Asn Thr Asn His Arg Ile Arg Glu Val Leu Lys Glu Leu Met Asn 
                805                 810                 815     
Asp Ala Ser Val Glu Gly Gly Val Arg Asp Tyr Ser Pro Ser Gln Gln 
            820                 825                 830         
Glu Ile Leu Lys Leu Tyr Glu Glu Gly Ile Tyr Gln Asn Pro Asn Thr 
        835                 840                 845             
Asn Tyr Leu Lys Val Asp Glu Asp Glu Ile Leu Lys Ile Arg Lys Lys 
    850                 855                 860                 
Asn Asn Pro Thr Gln Lys Glu Ile Gln Arg Tyr Lys Leu Trp Leu Glu 
865                 870                 875                 880 
Gln Gly Tyr Ile Ser Pro Tyr Thr Gly Lys Ile Ile Pro Leu Thr Lys 
                885                 890                 895     
Leu Phe Thr His Glu Tyr Gln Ile Glu His Ile Ile Pro Gln Ser Arg 
            900                 905                 910         
Tyr Tyr Asp Asn Ser Leu Gly Asn Lys Ile Ile Cys Glu Ser Glu Val 
        915                 920                 925             
Asn Glu Asp Lys Asp Asn Lys Thr Ala Tyr Glu Tyr Leu Lys Val Glu 
    930                 935                 940                 
Lys Gly Ser Ile Val Phe Gly His Lys Leu Leu Asn Leu Asp Glu Tyr 
945                 950                 955                 960 
Glu Ala His Val Asn Lys Tyr Phe Lys Lys Asn Lys Thr Lys Leu Lys 
                965                 970                 975     
Asn Leu Leu Ser Glu Asp Ile Pro Glu Gly Phe Ile Asn Arg Gln Leu 
            980                 985                 990         
Asn Asp Ser Arg Tyr Ile Ser Lys Leu Val Lys Gly Leu Leu Ser Asn 
        995                 1000                1005            
Ile Val Arg Glu Asn Gly Glu Gln Glu Ala Thr Ser Lys Asn Leu Ile 
    1010                1015                1020                
Pro Val Thr Gly Val Val Thr Ser Lys Leu Lys Gln Asp Trp Gly Leu 
1025                1030                1035                1040
Asn Asp Lys Trp Asn Glu Ile Ile Ala Pro Arg Phe Lys Arg Leu Asn 
                1045                1050                1055    
Lys Leu Thr Asn Ser Asn Asp Phe Gly Phe Trp Asp Asn Asp Ile Asn 
            1060                1065                1070        
Ala Phe Arg Ile Gln Val Pro Asp Ser Leu Ile Lys Gly Phe Ser Lys 
        1075                1080                1085            
Lys Arg Ile Asp His Arg His His Ala Leu Asp Ala Leu Val Val Ala 
    1090                1095                1100                
Cys Thr Ser Arg Asn His Thr His Tyr Leu Ser Ala Leu Asn Ala Glu 
1105                1110                1115                1120
Asn Lys Asn Tyr Ser Leu Arg Asp Lys Leu Val Ile Lys Asn Glu Asn 
                1125                1130                1135    
Gly Asp Tyr Thr Lys Thr Phe Gln Ile Pro Trp Gln Gly Phe Thr Ile 
            1140                1145                1150        
Glu Ala Lys Asn Asn Leu Glu Lys Thr Val Val Ser Phe Lys Lys Asn 
        1155                1160                1165            
Leu Arg Val Ile Asn Lys Thr Asn Asn Lys Phe Trp Ser Tyr Lys Asp 
    1170                1175                1180                
Glu Asn Gly Asn Leu Asn Leu Gly Lys Asp Gly Lys Pro Lys Lys Lys 
1185                1190                1195                1200
Leu Arg Lys Gln Thr Lys Gly Tyr Asn Trp Ala Ile Arg Lys Pro Leu 
                1205                1210                1215    
His Lys Glu Thr Val Ser Gly Ile Tyr Asn Ile Asn Ala Pro Lys Asn 
            1220                1225                1230        
Lys Ile Ala Thr Ser Val Arg Thr Leu Leu Thr Glu Ile Lys Asn Glu 
        1235                1240                1245            
Lys His Leu Ala Lys Ile Thr Asp Leu Arg Ile Arg Glu Thr Ile Leu 
    1250                1255                1260                
Pro Asn His Leu Lys His Tyr Leu Asn Asn Lys Gly Glu Ala Asn Phe 
1265                1270                1275                1280
Ser Glu Ala Phe Ser Gln Gly Gly Ile Glu Asp Leu Asn Lys Lys Ile 
                1285                1290                1295    
Thr Thr Leu Asn Glu Gly Lys Lys His Gln Pro Ile Tyr Arg Val Lys 
            1300                1305                1310        
Ile Phe Glu Val Gly Ser Lys Phe Ser Ile Ser Glu Asp Glu Asn Ser 
        1315                1320                1325            
Ala Lys Ser Lys Lys Tyr Val Glu Ala Ala Lys Gly Thr Asn Leu Phe 
    1330                1335                1340                
Phe Ala Ile Tyr Leu Asp Glu Glu Asn Lys Lys Arg Asn Tyr Glu Thr 
1345                1350                1355                1360
Ile Pro Leu Asn Glu Val Ile Thr His Gln Lys Gln Val Ala Gly Phe 
                1365                1370                1375    
Pro Lys Ser Glu Arg Leu Ser Val Gln Pro Asp Ser Gln Lys Gly Thr 
            1380                1385                1390        
Phe Leu Phe Thr Leu Ser Pro Asn Asp Leu Val Tyr Val Pro Asn Asn 
        1395                1400                1405            
Glu Glu Leu Glu Asn Arg Asp Leu Phe Asn Leu Gly Asn Leu Asn Val 
    1410                1415                1420                
Glu Gln Ile Ser Arg Ile Tyr Lys Phe Thr Asp Ser Ser Asp Lys Thr 
1425                1430                1435                1440
Cys Asn Phe Ile Pro Phe Gln Val Ser Lys Leu Ile Phe Asn Leu Lys 
                1445                1450                1455    
Lys Lys Glu Gln Lys Lys Leu Asp Val Asp Phe Ile Ile Gln Asn Glu 
            1460                1465                1470        
Phe Gly Leu Gly Ser Pro Gln Ser Lys Asn Gln Lys Ser Ile Asp Asp 
        1475                1480                1485            
Val Met Ile Lys Glu Lys Cys Ile Lys Leu Lys Ile Asp Arg Leu Gly 
    1490                1495                1500                
Asn Ile Ser Lys Ala 
1505                

<210> 27
<211> 1388
<212> PRT
<213> Artificial Sequence


<220> 
<223> Cas9 from Streptococcus thermophilus

<400> 27
Met Thr Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Thr Thr Asp Asn Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Ser Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              
Gly Val Leu Leu Phe Asp Ser Gly Ile Thr Ala Glu Gly Arg Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Thr Glu Met Ala Thr Leu Asp Asp Ala 
                85                  90                  95      
Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Val Pro Asp Asp Lys Arg 
            100                 105                 110         
Asp Ser Lys Tyr Pro Ile Phe Gly Asn Leu Val Glu Glu Lys Ala Tyr 
        115                 120                 125             
His Asp Glu Phe Pro Thr Ile Tyr His Leu Arg Lys Tyr Leu Ala Asp 
    130                 135                 140                 
Ser Thr Lys Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Phe Asn Ser 
                165                 170                 175     
Lys Asn Asn Asp Ile Gln Lys Asn Phe Gln Asp Phe Leu Asp Thr Tyr 
            180                 185                 190         
Asn Ala Ile Phe Glu Ser Asp Leu Ser Leu Glu Asn Ser Lys Gln Leu 
        195                 200                 205             
Glu Glu Ile Val Lys Asp Lys Ile Ser Lys Leu Glu Lys Lys Asp Arg 
    210                 215                 220                 
Ile Leu Lys Leu Phe Pro Gly Glu Lys Asn Ser Gly Ile Phe Ser Glu 
225                 230                 235                 240 
Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Arg Lys Cys Phe 
                245                 250                 255     
Asn Leu Asp Glu Lys Ala Ser Leu His Phe Ser Lys Glu Ser Tyr Asp 
            260                 265                 270         
Glu Asp Leu Glu Thr Leu Leu Gly Tyr Ile Gly Asp Asp Tyr Ser Asp 
        275                 280                 285             
Val Phe Leu Lys Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 
Phe Leu Thr Val Thr Asp Asn Glu Thr Glu Ala Pro Leu Ser Ser Ala 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Asn Glu His Lys Glu Asp Leu Ala Leu Leu Lys 
                325                 330                 335     
Glu Tyr Ile Arg Asn Ile Ser Leu Lys Thr Tyr Asn Glu Val Phe Lys 
            340                 345                 350         
Asp Asp Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Asn 
        355                 360                 365             
Gln Glu Asp Phe Tyr Val Tyr Leu Lys Lys Leu Leu Ala Glu Phe Glu 
    370                 375                 380                 
Gly Ala Asp Tyr Phe Leu Glu Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro Tyr Gln Ile His Leu 
                405                 410                 415     
Gln Glu Met Arg Ala Ile Leu Asp Lys Gln Ala Lys Phe Tyr Pro Phe 
            420                 425                 430         
Leu Ala Lys Asn Lys Glu Arg Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 
Ser Ile Arg Lys Arg Asn Glu Lys Ile Thr Pro Trp Asn Phe Glu Asp 
465                 470                 475                 480 
Val Ile Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     
Ser Phe Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Thr Phe Asn Val Tyr Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             
Phe Ile Ala Glu Ser Met Arg Asp Tyr Gln Phe Leu Asp Ser Lys Gln 
    530                 535                 540                 
Lys Lys Asp Ile Val Arg Leu Tyr Phe Lys Asp Lys Arg Lys Val Thr 
545                 550                 555                 560 
Asp Lys Asp Ile Ile Glu Tyr Leu His Ala Ile Tyr Gly Tyr Asp Gly 
                565                 570                 575     
Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ser Ser Leu Ser Thr 
            580                 585                 590         
Tyr His Asp Leu Leu Asn Ile Ile Asn Asp Lys Glu Phe Leu Asp Asp 
        595                 600                 605             
Ser Ser Asn Glu Ala Ile Ile Glu Glu Ile Ile His Thr Leu Thr Ile 
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ser Lys Phe Glu Asn 
625                 630                 635                 640 
Ile Phe Asp Lys Ser Val Leu Lys Lys Leu Ser Arg Arg His Tyr Thr 
                645                 650                 655     
Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Glu 
            660                 665                 670         
Lys Ser Gly Asn Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ile Ser 
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 
Lys Lys Ile Gln Lys Ala Gln Ile Ile Gly Asp Glu Asp Lys Gly Asn 
705                 710                 715                 720 
Ile Lys Glu Val Val Lys Ser Leu Pro Gly Ser Pro Ala Ile Lys Lys 
                725                 730                 735     
Gly Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met 
            740                 745                 750         
Gly Gly Arg Lys Pro Glu Ser Ile Val Val Glu Met Ala Arg Glu Asn 
        755                 760                 765             
Gln Tyr Thr Asn Gln Gly Lys Ser Asn Ser Gln Gln Arg Leu Lys Arg 
    770                 775                 780                 
Leu Glu Lys Ser Leu Lys Glu Leu Gly Ser Lys Ile Leu Lys Glu Asn 
785                 790                 795                 800 
Ile Pro Ala Lys Leu Ser Lys Ile Asp Asn Asn Ala Leu Gln Asn Asp 
                805                 810                 815     
Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly 
            820                 825                 830         
Asp Asp Leu Asp Ile Asp Arg Leu Ser Asn Tyr Asp Ile Asp His Ile 
        835                 840                 845             
Ile Pro Gln Ala Phe Leu Lys Asp Asn Ser Ile Asp Asn Lys Val Leu 
    850                 855                 860                 
Val Ser Ser Ala Ser Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Leu 
865                 870                 875                 880 
Glu Val Val Lys Lys Arg Lys Thr Phe Trp Tyr Gln Leu Leu Lys Ser 
                885                 890                 895     
Lys Leu Ile Ser Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg 
            900                 905                 910         
Gly Gly Leu Ser Pro Glu Asp Lys Ala Gly Phe Ile Gln Arg Gln Leu 
        915                 920                 925             
Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Arg Leu Leu Asp Glu 
    930                 935                 940                 
Lys Phe Asn Asn Lys Lys Asp Glu Asn Asn Arg Ala Val Arg Thr Val 
945                 950                 955                 960 
Lys Ile Ile Thr Leu Lys Ser Thr Leu Val Ser Gln Phe Arg Lys Asp 
                965                 970                 975     
Phe Glu Leu Tyr Lys Val Arg Glu Ile Asn Asp Phe His His Ala His 
            980                 985                 990         
Asp Ala Tyr Leu Asn Ala Val Val Ala Ser Ala Leu Leu Lys Lys Tyr 
        995                 1000                1005            
Pro Lys Leu Glu Pro Glu Phe Val Tyr Gly Asp Tyr Pro Lys Tyr Asn 
    1010                1015                1020                
Ser Phe Arg Glu Arg Lys Ser Ala Thr Glu Lys Val Tyr Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Ile Phe Lys Lys Ser Ile Ser Leu Ala Asp Gly Arg 
                1045                1050                1055    
Val Ile Glu Arg Pro Leu Ile Glu Val Asn Glu Glu Thr Gly Glu Ser 
            1060                1065                1070        
Val Trp Asn Lys Glu Ser Asp Leu Ala Thr Val Arg Arg Val Leu Ser 
        1075                1080                1085            
Tyr Pro Gln Val Asn Val Val Lys Lys Val Glu Glu Gln Asn His Gly 
    1090                1095                1100                
Leu Asp Arg Gly Lys Pro Lys Gly Leu Phe Asn Ala Asn Leu Ser Ser 
1105                1110                1115                1120
Lys Pro Lys Pro Asn Ser Asn Glu Asn Leu Val Gly Ala Lys Glu Tyr 
                1125                1130                1135    
Leu Asp Pro Lys Lys Tyr Gly Gly Tyr Ala Gly Ile Ser Asn Ser Phe 
            1140                1145                1150        
Thr Val Leu Val Lys Gly Thr Ile Glu Lys Gly Ala Lys Lys Lys Ile 
        1155                1160                1165            
Thr Asn Val Leu Glu Phe Gln Gly Ile Ser Ile Leu Asp Arg Ile Asn 
    1170                1175                1180                
Tyr Arg Lys Asp Lys Leu Asn Phe Leu Leu Glu Lys Gly Tyr Lys Asp 
1185                1190                1195                1200
Ile Glu Leu Ile Ile Glu Leu Pro Lys Tyr Ser Leu Phe Glu Leu Ser 
                1205                1210                1215    
Asp Gly Ser Arg Arg Met Leu Ala Ser Ile Leu Ser Thr Asn Asn Lys 
            1220                1225                1230        
Arg Gly Glu Ile His Lys Gly Asn Gln Ile Phe Leu Ser Gln Lys Phe 
        1235                1240                1245            
Val Lys Leu Leu Tyr His Ala Lys Arg Ile Ser Asn Thr Ile Asn Glu 
    1250                1255                1260                
Asn His Arg Lys Tyr Val Glu Asn His Lys Lys Glu Phe Glu Glu Leu 
1265                1270                1275                1280
Phe Tyr Tyr Ile Leu Glu Phe Asn Glu Asn Tyr Val Gly Ala Lys Lys 
                1285                1290                1295    
Asn Gly Lys Leu Leu Asn Ser Ala Phe Gln Ser Trp Gln Asn His Ser 
            1300                1305                1310        
Ile Asp Glu Leu Cys Ser Ser Phe Ile Gly Pro Thr Gly Ser Glu Arg 
        1315                1320                1325            
Lys Gly Leu Phe Glu Leu Thr Ser Arg Gly Ser Ala Ala Asp Phe Glu 
    1330                1335                1340                
Phe Leu Gly Val Lys Ile Pro Arg Tyr Arg Asp Tyr Thr Pro Ser Ser 
1345                1350                1355                1360
Leu Leu Lys Asp Ala Thr Leu Ile His Gln Ser Val Thr Gly Leu Tyr 
                1365                1370                1375    
Glu Thr Arg Ile Asp Leu Ala Lys Leu Gly Glu Gly 
            1380                1385            

<210> 28
<211> 1334
<212> PRT
<213> Artificial Sequence


<220> 
<223> Cas9 from Listeria innocua

<400> 28
Met Lys Lys Pro Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Leu Thr Asp Gln Tyr Asp Leu Val Lys Arg Lys Met 
            20                  25                  30          
Lys Ile Ala Gly Asp Ser Glu Lys Lys Gln Ile Lys Lys Asn Phe Trp 
        35                  40                  45              
Gly Val Arg Leu Phe Asp Glu Gly Gln Thr Ala Ala Asp Arg Arg Met 
    50                  55                  60                  
Ala Arg Thr Ala Arg Arg Arg Ile Glu Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  
Tyr Leu Gln Gly Ile Phe Ala Glu Glu Met Ser Lys Thr Asp Ala Asn 
                85                  90                  95      
Phe Phe Cys Arg Leu Ser Asp Ser Phe Tyr Val Asp Asn Glu Lys Arg 
            100                 105                 110         
Asn Ser Arg His Pro Phe Phe Ala Thr Ile Glu Glu Glu Val Glu Tyr 
        115                 120                 125             
His Lys Asn Tyr Pro Thr Ile Tyr His Leu Arg Glu Glu Leu Val Asn 
    130                 135                 140                 
Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Ala Leu Asp Thr 
                165                 170                 175     
Gln Asn Thr Ser Val Asp Gly Ile Tyr Lys Gln Phe Ile Gln Thr Tyr 
            180                 185                 190         
Asn Gln Val Phe Ala Ser Gly Ile Glu Asp Gly Ser Leu Lys Lys Leu 
        195                 200                 205             
Glu Asp Asn Lys Asp Val Ala Lys Ile Leu Val Glu Lys Val Thr Arg 
    210                 215                 220                 
Lys Glu Lys Leu Glu Arg Ile Leu Lys Leu Tyr Pro Gly Glu Lys Ser 
225                 230                 235                 240 
Ala Gly Met Phe Ala Gln Phe Ile Ser Leu Ile Val Gly Ser Lys Gly 
                245                 250                 255     
Asn Phe Gln Lys Pro Phe Asp Leu Ile Glu Lys Ser Asp Ile Glu Cys 
            260                 265                 270         
Ala Lys Asp Ser Tyr Glu Glu Asp Leu Glu Ser Leu Leu Ala Leu Ile 
        275                 280                 285             
Gly Asp Glu Tyr Ala Glu Leu Phe Val Ala Ala Lys Asn Ala Tyr Ser 
    290                 295                 300                 
Ala Val Val Leu Ser Ser Ile Ile Thr Val Ala Glu Thr Glu Thr Asn 
305                 310                 315                 320 
Ala Lys Leu Ser Ala Ser Met Ile Glu Arg Phe Asp Thr His Glu Glu 
                325                 330                 335     
Asp Leu Gly Glu Leu Lys Ala Phe Ile Lys Leu His Leu Pro Lys His 
            340                 345                 350         
Tyr Glu Glu Ile Phe Ser Asn Thr Glu Lys His Gly Tyr Ala Gly Tyr 
        355                 360                 365             
Ile Asp Gly Lys Thr Lys Gln Ala Asp Phe Tyr Lys Tyr Met Lys Met 
    370                 375                 380                 
Thr Leu Glu Asn Ile Glu Gly Ala Asp Tyr Phe Ile Ala Lys Ile Glu 
385                 390                 395                 400 
Lys Glu Asn Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ala Ile 
                405                 410                 415     
Pro His Gln Leu His Leu Glu Glu Leu Glu Ala Ile Leu His Gln Gln 
            420                 425                 430         
Ala Lys Tyr Tyr Pro Phe Leu Lys Glu Asn Tyr Asp Lys Ile Lys Ser 
        435                 440                 445             
Leu Val Thr Phe Arg Ile Pro Tyr Phe Val Gly Pro Leu Ala Asn Gly 
    450                 455                 460                 
Gln Ser Glu Phe Ala Trp Leu Thr Arg Lys Ala Asp Gly Glu Ile Arg 
465                 470                 475                 480 
Pro Trp Asn Ile Glu Glu Lys Val Asp Phe Gly Lys Ser Ala Val Asp 
                485                 490                 495     
Phe Ile Glu Lys Met Thr Asn Lys Asp Thr Tyr Leu Pro Lys Glu Asn 
            500                 505                 510         
Val Leu Pro Lys His Ser Leu Cys Tyr Gln Lys Tyr Leu Val Tyr Asn 
        515                 520                 525             
Glu Leu Thr Lys Val Arg Tyr Ile Asn Asp Gln Gly Lys Thr Ser Tyr 
    530                 535                 540                 
Phe Ser Gly Gln Glu Lys Glu Gln Ile Phe Asn Asp Leu Phe Lys Gln 
545                 550                 555                 560 
Lys Arg Lys Val Lys Lys Lys Asp Leu Glu Leu Phe Leu Arg Asn Met 
                565                 570                 575     
Ser His Val Glu Ser Pro Thr Ile Glu Gly Leu Glu Asp Ser Phe Asn 
            580                 585                 590         
Ser Ser Tyr Ser Thr Tyr His Asp Leu Leu Lys Val Gly Ile Lys Gln 
        595                 600                 605             
Glu Ile Leu Asp Asn Pro Val Asn Thr Glu Met Leu Glu Asn Ile Val 
    610                 615                 620                 
Lys Ile Leu Thr Val Phe Glu Asp Lys Arg Met Ile Lys Glu Gln Leu 
625                 630                 635                 640 
Gln Gln Phe Ser Asp Val Leu Asp Gly Val Val Leu Lys Lys Leu Glu 
                645                 650                 655     
Arg Arg His Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Met 
            660                 665                 670         
Gly Ile Arg Asp Lys Gln Ser His Leu Thr Ile Leu Asp Tyr Leu Met 
        675                 680                 685             
Asn Asp Asp Gly Leu Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Ser 
    690                 695                 700                 
Asn Leu Ser Phe Lys Ser Ile Ile Glu Lys Glu Gln Val Thr Thr Ala 
705                 710                 715                 720 
Asp Lys Asp Ile Gln Ser Ile Val Ala Asp Leu Ala Gly Ser Pro Ala 
                725                 730                 735     
Ile Lys Lys Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val 
            740                 745                 750         
Ser Val Met Gly Tyr Pro Pro Gln Thr Ile Val Val Glu Met Ala Arg 
        755                 760                 765             
Glu Asn Gln Thr Thr Gly Lys Gly Lys Asn Asn Ser Arg Pro Arg Tyr 
    770                 775                 780                 
Lys Ser Leu Glu Lys Ala Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys 
785                 790                 795                 800 
Glu His Pro Thr Asp Asn Gln Glu Leu Arg Asn Asn Arg Leu Tyr Leu 
                805                 810                 815     
Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Asp Leu Asp 
            820                 825                 830         
Ile His Asn Leu Ser Asn Tyr Asp Ile Asp His Ile Val Pro Gln Ser 
        835                 840                 845             
Phe Ile Thr Asp Asn Ser Ile Asp Asn Leu Val Leu Thr Ser Ser Ala 
    850                 855                 860                 
Gly Asn Arg Glu Lys Gly Asp Asp Val Pro Pro Leu Glu Ile Val Arg 
865                 870                 875                 880 
Lys Arg Lys Val Phe Trp Glu Lys Leu Tyr Gln Gly Asn Leu Met Ser 
                885                 890                 895     
Lys Arg Lys Phe Asp Tyr Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr 
            900                 905                 910         
Glu Ala Asp Lys Ala Arg Phe Ile His Arg Gln Leu Val Glu Thr Arg 
        915                 920                 925             
Gln Ile Thr Lys Asn Val Ala Asn Ile Leu His Gln Arg Phe Asn Tyr 
    930                 935                 940                 
Glu Lys Asp Asp His Gly Asn Thr Met Lys Gln Val Arg Ile Val Thr 
945                 950                 955                 960 
Leu Lys Ser Ala Leu Val Ser Gln Phe Arg Lys Gln Phe Gln Leu Tyr 
                965                 970                 975     
Lys Val Arg Asp Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu 
            980                 985                 990         
Asn Gly Val Val Ala Asn Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu 
        995                 1000                1005            
Pro Glu Phe Val Tyr Gly Asp Tyr His Gln Phe Asp Trp Phe Lys Ala 
    1010                1015                1020                
Asn Lys Ala Thr Ala Lys Lys Gln Phe Tyr Thr Asn Ile Met Leu Phe 
1025                1030                1035                1040
Phe Ala Gln Lys Asp Arg Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp 
                1045                1050                1055    
Asp Lys Lys Tyr Leu Asp Thr Val Lys Lys Val Met Ser Tyr Arg Gln 
            1060                1065                1070        
Met Asn Ile Val Lys Lys Thr Glu Ile Gln Lys Gly Glu Phe Ser Lys 
        1075                1080                1085            
Ala Thr Ile Lys Pro Lys Gly Asn Ser Ser Lys Leu Ile Pro Arg Lys 
    1090                1095                1100                
Thr Asn Trp Asp Pro Met Lys Tyr Gly Gly Leu Asp Ser Pro Asn Met 
1105                1110                1115                1120
Ala Tyr Ala Val Val Ile Glu Tyr Ala Lys Gly Lys Asn Lys Leu Val 
                1125                1130                1135    
Phe Glu Lys Lys Ile Ile Arg Val Thr Ile Met Glu Arg Lys Ala Phe 
            1140                1145                1150        
Glu Lys Asp Glu Lys Ala Phe Leu Glu Glu Gln Gly Tyr Arg Gln Pro 
        1155                1160                1165            
Lys Val Leu Ala Lys Leu Pro Lys Tyr Thr Leu Tyr Glu Cys Glu Glu 
    1170                1175                1180                
Gly Arg Arg Arg Met Leu Ala Ser Ala Asn Glu Ala Gln Lys Gly Asn 
1185                1190                1195                1200
Gln Gln Val Leu Pro Asn His Leu Val Thr Leu Leu His His Ala Ala 
                1205                1210                1215    
Asn Cys Glu Val Ser Asp Gly Lys Ser Leu Asp Tyr Ile Glu Ser Asn 
            1220                1225                1230        
Arg Glu Met Phe Ala Glu Leu Leu Ala His Val Ser Glu Phe Ala Lys 
        1235                1240                1245            
Arg Tyr Thr Leu Ala Glu Ala Asn Leu Asn Lys Ile Asn Gln Leu Phe 
    1250                1255                1260                
Glu Gln Asn Lys Glu Gly Asp Ile Lys Ala Ile Ala Gln Ser Phe Val 
1265                1270                1275                1280
Asp Leu Met Ala Phe Asn Ala Met Gly Ala Pro Ala Ser Phe Lys Phe 
                1285                1290                1295    
Phe Glu Thr Thr Ile Glu Arg Lys Arg Tyr Asn Asn Leu Lys Glu Leu 
            1300                1305                1310        
Leu Asn Ser Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr Glu Ser 
        1315                1320                1325            
Arg Lys Arg Leu Asp Asp 
    1330                

<210> 29
<211> 984
<212> PRT
<213> Artificial Sequence


<220> 
<223> Cas9 from Campylobacter jejuni

<400> 29
Met Ala Arg Ile Leu Ala Phe Asp Ile Gly Ile Ser Ser Ile Gly Trp 
1               5                   10                  15      
Ala Phe Ser Glu Asn Asp Glu Leu Lys Asp Cys Gly Val Arg Ile Phe 
            20                  25                  30          
Thr Lys Val Glu Asn Pro Lys Thr Gly Glu Ser Leu Ala Leu Pro Arg 
        35                  40                  45              
Arg Leu Ala Arg Ser Ala Arg Lys Arg Leu Ala Arg Arg Lys Ala Arg 
    50                  55                  60                  
Leu Asn His Leu Lys His Leu Ile Ala Asn Glu Phe Lys Leu Asn Tyr 
65                  70                  75                  80  
Glu Asp Tyr Gln Ser Phe Asp Glu Ser Leu Ala Lys Ala Tyr Lys Gly 
                85                  90                  95      
Ser Leu Ile Ser Pro Tyr Glu Leu Arg Phe Arg Ala Leu Asn Glu Leu 
            100                 105                 110         
Leu Ser Lys Gln Asp Phe Ala Arg Val Ile Leu His Ile Ala Lys Arg 
        115                 120                 125             
Arg Gly Tyr Asp Asp Ile Lys Asn Ser Asp Asp Lys Glu Lys Gly Ala 
    130                 135                 140                 
Ile Leu Lys Ala Ile Lys Gln Asn Glu Glu Lys Leu Ala Asn Tyr Gln 
145                 150                 155                 160 
Ser Val Gly Glu Tyr Leu Tyr Lys Glu Tyr Phe Gln Lys Phe Lys Glu 
                165                 170                 175     
Asn Ser Lys Glu Phe Thr Asn Val Arg Asn Lys Lys Glu Ser Tyr Glu 
            180                 185                 190         
Arg Cys Ile Ala Gln Ser Phe Leu Lys Asp Glu Leu Lys Leu Ile Phe 
        195                 200                 205             
Lys Lys Gln Arg Glu Phe Gly Phe Ser Phe Ser Lys Lys Phe Glu Glu 
    210                 215                 220                 
Glu Val Leu Ser Val Ala Phe Tyr Lys Arg Ala Leu Lys Asp Phe Ser 
225                 230                 235                 240 
His Leu Val Gly Asn Cys Ser Phe Phe Thr Asp Glu Lys Arg Ala Pro 
                245                 250                 255     
Lys Asn Ser Pro Leu Ala Phe Met Phe Val Ala Leu Thr Arg Ile Ile 
            260                 265                 270         
Asn Leu Leu Asn Asn Leu Lys Asn Thr Glu Gly Ile Leu Tyr Thr Lys 
        275                 280                 285             
Asp Asp Leu Asn Ala Leu Leu Asn Glu Val Leu Lys Asn Gly Thr Leu 
    290                 295                 300                 
Thr Tyr Lys Gln Thr Lys Lys Leu Leu Gly Leu Ser Asp Asp Tyr Glu 
305                 310                 315                 320 
Phe Lys Gly Glu Lys Gly Thr Tyr Phe Ile Glu Phe Lys Lys Tyr Lys 
                325                 330                 335     
Glu Phe Ile Lys Ala Leu Gly Glu His Asn Leu Ser Gln Asp Asp Leu 
            340                 345                 350         
Asn Glu Ile Ala Lys Asp Ile Thr Leu Ile Lys Asp Glu Ile Lys Leu 
        355                 360                 365             
Lys Lys Ala Leu Ala Lys Tyr Asp Leu Asn Gln Asn Gln Ile Asp Ser 
    370                 375                 380                 
Leu Ser Lys Leu Glu Phe Lys Asp His Leu Asn Ile Ser Phe Lys Ala 
385                 390                 395                 400 
Leu Lys Leu Val Thr Pro Leu Met Leu Glu Gly Lys Lys Tyr Asp Glu 
                405                 410                 415     
Ala Cys Asn Glu Leu Asn Leu Lys Val Ala Ile Asn Glu Asp Lys Lys 
            420                 425                 430         
Asp Phe Leu Pro Ala Phe Asn Glu Thr Tyr Tyr Lys Asp Glu Val Thr 
        435                 440                 445             
Asn Pro Val Val Leu Arg Ala Ile Lys Glu Tyr Arg Lys Val Leu Asn 
    450                 455                 460                 
Ala Leu Leu Lys Lys Tyr Gly Lys Val His Lys Ile Asn Ile Glu Leu 
465                 470                 475                 480 
Ala Arg Glu Val Gly Lys Asn His Ser Gln Arg Ala Lys Ile Glu Lys 
                485                 490                 495     
Glu Gln Asn Glu Asn Tyr Lys Ala Lys Lys Asp Ala Glu Leu Glu Cys 
            500                 505                 510         
Glu Lys Leu Gly Leu Lys Ile Asn Ser Lys Asn Ile Leu Lys Leu Arg 
        515                 520                 525             
Leu Phe Lys Glu Gln Lys Glu Phe Cys Ala Tyr Ser Gly Glu Lys Ile 
    530                 535                 540                 
Lys Ile Ser Asp Leu Gln Asp Glu Lys Met Leu Glu Ile Asp His Ile 
545                 550                 555                 560 
Tyr Pro Tyr Ser Arg Ser Phe Asp Asp Ser Tyr Met Asn Lys Val Leu 
                565                 570                 575     
Val Phe Thr Lys Gln Asn Gln Glu Lys Leu Asn Gln Thr Pro Phe Glu 
            580                 585                 590         
Ala Phe Gly Asn Asp Ser Ala Lys Trp Gln Lys Ile Glu Val Leu Ala 
        595                 600                 605             
Lys Asn Leu Pro Thr Lys Lys Gln Lys Arg Ile Leu Asp Lys Asn Tyr 
    610                 615                 620                 
Lys Asp Lys Glu Gln Lys Asn Phe Lys Asp Arg Asn Leu Asn Asp Thr 
625                 630                 635                 640 
Arg Tyr Ile Ala Arg Leu Val Leu Asn Tyr Thr Lys Asp Tyr Leu Asp 
                645                 650                 655     
Phe Leu Pro Leu Ser Asp Asp Glu Asn Thr Lys Leu Asn Asp Thr Gln 
            660                 665                 670         
Lys Gly Ser Lys Val His Val Glu Ala Lys Ser Gly Met Leu Thr Ser 
        675                 680                 685             
Ala Leu Arg His Thr Trp Gly Phe Ser Ala Lys Asp Arg Asn Asn His 
    690                 695                 700                 
Leu His His Ala Ile Asp Ala Val Ile Ile Ala Tyr Ala Asn Asn Ser 
705                 710                 715                 720 
Ile Val Lys Ala Phe Ser Asp Phe Lys Lys Glu Gln Glu Ser Asn Ser 
                725                 730                 735     
Ala Glu Leu Tyr Ala Lys Lys Ile Ser Glu Leu Asp Tyr Lys Asn Lys 
            740                 745                 750         
Arg Lys Phe Phe Glu Pro Phe Ser Gly Phe Arg Gln Lys Val Leu Asp 
        755                 760                 765             
Lys Ile Asp Glu Ile Phe Val Ser Lys Pro Glu Arg Lys Lys Pro Ser 
    770                 775                 780                 
Gly Ala Leu His Glu Glu Thr Phe Arg Lys Glu Glu Glu Phe Tyr Gln 
785                 790                 795                 800 
Ser Tyr Gly Gly Lys Glu Gly Val Leu Lys Ala Leu Glu Leu Gly Lys 
                805                 810                 815     
Ile Arg Lys Val Asn Gly Lys Ile Val Lys Asn Gly Asp Met Phe Arg 
            820                 825                 830         
Val Asp Ile Phe Lys His Lys Lys Thr Asn Lys Phe Tyr Ala Val Pro 
        835                 840                 845             
Ile Tyr Thr Met Asp Phe Ala Leu Lys Val Leu Pro Asn Lys Ala Val 
    850                 855                 860                 
Ala Arg Ser Lys Lys Gly Glu Ile Lys Asp Trp Ile Leu Met Asp Glu 
865                 870                 875                 880 
Asn Tyr Glu Phe Cys Phe Ser Leu Tyr Lys Asp Ser Leu Ile Leu Ile 
                885                 890                 895     
Gln Thr Lys Asp Met Gln Glu Pro Glu Phe Val Tyr Tyr Asn Ala Phe 
            900                 905                 910         
Thr Ser Ser Thr Val Ser Leu Ile Val Ser Lys His Asp Asn Lys Phe 
        915                 920                 925             
Glu Thr Leu Ser Lys Asn Gln Lys Ile Leu Phe Lys Asn Ala Asn Glu 
    930                 935                 940                 
Lys Glu Val Ile Ala Lys Ser Ile Gly Ile Gln Asn Leu Lys Val Phe 
945                 950                 955                 960 
Glu Lys Tyr Ile Val Ser Ala Leu Gly Glu Val Thr Lys Ala Glu Phe 
                965                 970                 975     
Arg Gln Arg Glu Asp Phe Lys Lys 
            980                 

<210> 30
<211> 1082
<212> PRT
<213> Artificial Sequence


<220> 
<223> Cas9 from Neisseria. meningitidis

<400> 30
Met Ala Ala Phe Lys Pro Asn Pro Ile Asn Tyr Ile Leu Gly Leu Asp 
1               5                   10                  15      
Ile Gly Ile Ala Ser Val Gly Trp Ala Met Val Glu Ile Asp Glu Asp 
            20                  25                  30          
Glu Asn Pro Ile Cys Leu Ile Asp Leu Gly Val Arg Val Phe Glu Arg 
        35                  40                  45              
Ala Glu Val Pro Lys Thr Gly Asp Ser Leu Ala Met Ala Arg Arg Leu 
    50                  55                  60                  
Ala Arg Ser Val Arg Arg Leu Thr Arg Arg Arg Ala His Arg Leu Leu 
65                  70                  75                  80  
Arg Ala Arg Arg Leu Leu Lys Arg Glu Gly Val Leu Gln Ala Ala Asp 
                85                  90                  95      
Phe Asp Glu Asn Gly Leu Ile Lys Ser Leu Pro Asn Thr Pro Trp Gln 
            100                 105                 110         
Leu Arg Ala Ala Ala Leu Asp Arg Lys Leu Thr Pro Leu Glu Trp Ser 
        115                 120                 125             
Ala Val Leu Leu His Leu Ile Lys His Arg Gly Tyr Leu Ser Gln Arg 
    130                 135                 140                 
Lys Asn Glu Gly Glu Thr Ala Asp Lys Glu Leu Gly Ala Leu Leu Lys 
145                 150                 155                 160 
Gly Val Ala Asp Asn Ala His Ala Leu Gln Thr Gly Asp Phe Arg Thr 
                165                 170                 175     
Pro Ala Glu Leu Ala Leu Asn Lys Phe Glu Lys Glu Ser Gly His Ile 
            180                 185                 190         
Arg Asn Gln Arg Gly Asp Tyr Ser His Thr Phe Ser Arg Lys Asp Leu 
        195                 200                 205             
Gln Ala Glu Leu Ile Leu Leu Phe Glu Lys Gln Lys Glu Phe Gly Asn 
    210                 215                 220                 
Pro His Val Ser Gly Gly Leu Lys Glu Gly Ile Glu Thr Leu Leu Met 
225                 230                 235                 240 
Thr Gln Arg Pro Ala Leu Ser Gly Asp Ala Val Gln Lys Met Leu Gly 
                245                 250                 255     
His Cys Thr Phe Glu Pro Ala Glu Pro Lys Ala Ala Lys Asn Thr Tyr 
            260                 265                 270         
Thr Ala Glu Arg Phe Ile Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile 
        275                 280                 285             
Leu Glu Gln Gly Ser Glu Arg Pro Leu Thr Asp Thr Glu Arg Ala Thr 
    290                 295                 300                 
Leu Met Asp Glu Pro Tyr Arg Lys Ser Lys Leu Thr Tyr Ala Gln Ala 
305                 310                 315                 320 
Arg Lys Leu Leu Gly Leu Glu Asp Thr Ala Phe Phe Lys Gly Leu Arg 
                325                 330                 335     
Tyr Gly Lys Asp Asn Ala Glu Ala Ser Thr Leu Met Glu Met Lys Ala 
            340                 345                 350         
Tyr His Ala Ile Ser Arg Ala Leu Glu Lys Glu Gly Leu Lys Asp Lys 
        355                 360                 365             
Lys Ser Pro Leu Asn Leu Ser Pro Glu Leu Gln Asp Glu Ile Gly Thr 
    370                 375                 380                 
Ala Phe Ser Leu Phe Lys Thr Asp Glu Asp Ile Thr Gly Arg Leu Lys 
385                 390                 395                 400 
Asp Arg Ile Gln Pro Glu Ile Leu Glu Ala Leu Leu Lys His Ile Ser 
                405                 410                 415     
Phe Asp Lys Phe Val Gln Ile Ser Leu Lys Ala Leu Arg Arg Ile Val 
            420                 425                 430         
Pro Leu Met Glu Gln Gly Lys Arg Tyr Asp Glu Ala Cys Ala Glu Ile 
        435                 440                 445             
Tyr Gly Asp His Tyr Gly Lys Lys Asn Thr Glu Glu Lys Ile Tyr Leu 
    450                 455                 460                 
Pro Pro Ile Pro Ala Asp Glu Ile Arg Asn Pro Val Val Leu Arg Ala 
465                 470                 475                 480 
Leu Ser Gln Ala Arg Lys Val Ile Asn Gly Val Val Arg Arg Tyr Gly 
                485                 490                 495     
Ser Pro Ala Arg Ile His Ile Glu Thr Ala Arg Glu Val Gly Lys Ser 
            500                 505                 510         
Phe Lys Asp Arg Lys Glu Ile Glu Lys Arg Gln Glu Glu Asn Arg Lys 
        515                 520                 525             
Asp Arg Glu Lys Ala Ala Ala Lys Phe Arg Glu Tyr Phe Pro Asn Phe 
    530                 535                 540                 
Val Gly Glu Pro Lys Ser Lys Asp Ile Leu Lys Leu Arg Leu Tyr Glu 
545                 550                 555                 560 
Gln Gln His Gly Lys Cys Leu Tyr Ser Gly Lys Glu Ile Asn Leu Gly 
                565                 570                 575     
Arg Leu Asn Glu Lys Gly Tyr Val Glu Ile Asp His Ala Leu Pro Phe 
            580                 585                 590         
Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu Val Leu Gly 
        595                 600                 605             
Ser Glu Asn Gln Asn Lys Gly Asn Gln Thr Pro Tyr Glu Tyr Phe Asn 
    610                 615                 620                 
Gly Lys Asp Asn Ser Arg Glu Trp Gln Glu Phe Lys Ala Arg Val Glu 
625                 630                 635                 640 
Thr Ser Arg Phe Pro Arg Ser Lys Lys Gln Arg Ile Leu Leu Gln Lys 
                645                 650                 655     
Phe Asp Glu Asp Gly Phe Lys Glu Arg Asn Leu Asn Asp Thr Arg Tyr 
            660                 665                 670         
Val Asn Arg Phe Leu Cys Gln Phe Val Ala Asp Arg Met Arg Leu Thr 
        675                 680                 685             
Gly Lys Gly Lys Lys Arg Val Phe Ala Ser Asn Gly Gln Ile Thr Asn 
    690                 695                 700                 
Leu Leu Arg Gly Phe Trp Gly Leu Arg Lys Val Arg Ala Glu Asn Asp 
705                 710                 715                 720 
Arg His His Ala Leu Asp Ala Val Val Val Ala Cys Ser Thr Val Ala 
                725                 730                 735     
Met Gln Gln Lys Ile Thr Arg Phe Val Arg Tyr Lys Glu Met Asn Ala 
            740                 745                 750         
Phe Asp Gly Lys Thr Ile Asp Lys Glu Thr Gly Glu Val Leu His Gln 
        755                 760                 765             
Lys Thr His Phe Pro Gln Pro Trp Glu Phe Phe Ala Gln Glu Val Met 
    770                 775                 780                 
Ile Arg Val Phe Gly Lys Pro Asp Gly Lys Pro Glu Phe Glu Glu Ala 
785                 790                 795                 800 
Asp Thr Pro Glu Lys Leu Arg Thr Leu Leu Ala Glu Lys Leu Ser Ser 
                805                 810                 815     
Arg Pro Glu Ala Val His Glu Tyr Val Thr Pro Leu Phe Val Ser Arg 
            820                 825                 830         
Ala Pro Asn Arg Lys Met Ser Gly Gln Gly His Met Glu Thr Val Lys 
        835                 840                 845             
Ser Ala Lys Arg Leu Asp Glu Gly Val Ser Val Leu Arg Val Pro Leu 
    850                 855                 860                 
Thr Gln Leu Lys Leu Lys Asp Leu Glu Lys Met Val Asn Arg Glu Arg 
865                 870                 875                 880 
Glu Pro Lys Leu Tyr Glu Ala Leu Lys Ala Arg Leu Glu Ala His Lys 
                885                 890                 895     
Asp Asp Pro Ala Lys Ala Phe Ala Glu Pro Phe Tyr Lys Tyr Asp Lys 
            900                 905                 910         
Ala Gly Asn Arg Thr Gln Gln Val Lys Ala Val Arg Val Glu Gln Val 
        915                 920                 925             
Gln Lys Thr Gly Val Trp Val Arg Asn His Asn Gly Ile Ala Asp Asn 
    930                 935                 940                 
Ala Thr Met Val Arg Val Asp Val Phe Glu Lys Gly Asp Lys Tyr Tyr 
945                 950                 955                 960 
Leu Val Pro Ile Tyr Ser Trp Gln Val Ala Lys Gly Ile Leu Pro Asp 
                965                 970                 975     
Arg Ala Val Val Gln Gly Lys Asp Glu Glu Asp Trp Gln Leu Ile Asp 
            980                 985                 990         
Asp Ser Phe Asn Phe Lys Phe Ser Leu His Pro Asn Asp Leu Val Glu 
        995                 1000                1005            
Val Ile Thr Lys Lys Ala Arg Met Phe Gly Tyr Phe Ala Ser Cys His 
    1010                1015                1020                
Arg Gly Thr Gly Asn Ile Asn Ile Arg Ile His Asp Leu Asp His Lys 
1025                1030                1035                1040
Ile Gly Lys Asn Gly Ile Leu Glu Gly Ile Gly Val Lys Thr Ala Leu 
                1045                1050                1055    
Ser Phe Gln Lys Tyr Gln Ile Asp Glu Leu Gly Lys Glu Ile Arg Pro 
            1060                1065                1070        
Cys Arg Leu Lys Lys Arg Pro Pro Val Arg 
        1075                1080        

<210> 31
<211> 1367
<212> PRT
<213> Artificial Sequence


<220> 
<223> Cas9 from Streptococcus pyogenes

<400> 31
Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Asp Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Gly Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         
His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             
His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Ala Asp 
    130                 135                 140                 
Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     
Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Ile Tyr 
            180                 185                 190         
Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Arg Val Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ala Gln Leu Pro Gly Glu Lys Arg Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     
Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Arg Val Asn Ser Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     
Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         
Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             
Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 
Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 
Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 
Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     
Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Ala Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             
Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Gly Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 
His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 
Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly His Ser Leu 
705                 710                 715                 720 
His Glu Gln Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Thr Val Lys Ile Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr 
        755                 760                 765             
Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile Glu 
    770                 775                 780                 
Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro Val 
785                 790                 795                 800 
Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu Gln 
                805                 810                 815     
Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg Leu 
            820                 825                 830         
Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Ile Lys Asp 
        835                 840                 845             
Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg Gly 
    850                 855                 860                 
Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys Asn 
865                 870                 875                 880 
Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys Phe 
                885                 890                 895     
Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp Lys 
            900                 905                 910         
Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys 
        915                 920                 925             
His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp Glu 
    930                 935                 940                 
Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser Lys 
945                 950                 955                 960 
Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg Glu 
                965                 970                 975     
Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val 
            980                 985                 990         
Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu Glu Ser Glu Phe Val 
        995                 1000                1005            
Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys Met Ile Ala Lys Ser 
    1010                1015                1020                
Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser Asn 
1025                1030                1035                1040
Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu Ala Asn Gly Glu Ile 
                1045                1050                1055    
Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu Thr Gly Glu Ile Val 
            1060                1065                1070        
Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg Lys Val Leu Ser Met 
        1075                1080                1085            
Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly Phe 
    1090                1095                1100                
Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser Asp Lys Leu Ile Ala 
1105                1110                1115                1120
Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro 
                1125                1130                1135    
Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu Lys Gly Lys 
            1140                1145                1150        
Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu Gly Ile Thr Ile Met 
        1155                1160                1165            
Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp Phe Leu Glu Ala Lys 
    1170                1175                1180                
Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile Lys Leu Pro Lys Tyr 
1185                1190                1195                1200
Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg Met Leu Ala Ser Ala 
                1205                1210                1215    
Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu Pro Ser Lys Tyr Val 
            1220                1225                1230        
Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys Leu Lys Gly Ser Pro 
        1235                1240                1245            
Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu Gln His Lys His Tyr 
    1250                1255                1260                
Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe Ser Lys Arg Val Ile 
1265                1270                1275                1280
Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser Ala Tyr Asn Lys His 
                1285                1290                1295    
Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn Ile Ile His Leu Phe 
            1300                1305                1310        
Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe Lys Tyr Phe Asp Thr 
        1315                1320                1325            
Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu Asp Ala 
    1330                1335                1340                
Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile Asp 
1345                1350                1355                1360
Leu Ser Gln Leu Gly Gly Asp 
                1365        

<210> 32
<211> 528
<212> DNA
<213> Artificial Sequence


<220> 
<223> zfp coding sequence

<400> 32
atggcccagg ctgctcttga gcccggagag aaaccctaca agtgcccgga gtgcggaaag      60

tccttctctg agcggagtca cctccgagag caccagcgga ctcatacggg cgaaaaacca     120

tacaagtgcc cagaatgtgg taaatctttt tctcgggctg acaacctgac tgaacatcag     180

cgcacgcaca ccggtgaaaa accttacaag tgtccagagt gtggcaagag cttttctagt     240

agaaggacct gtcgagcgca tcagcggact cacaccggcg aaaaacccta taagtgtccg     300

gaatgtggaa agagctttag ccgcaacgac acccttactg aacaccagcg aacacacacg     360

ggagaaaaac catataaatg tccggaatgt ggcaaaagtt ttagtcggag tgataaactt     420

acggagcacc aacggacaca caccggagag aagccatata agtgtcctga atgtggaaag     480

tccttctcac agcttgctca tctgcgagca catcagcgca cacacacc                  528


<210> 33
<211> 176
<212> PRT
<213> Artificial Sequence


<220> 
<223> ZFP amino acid sequence

<400> 33
Met Ala Gln Ala Ala Leu Glu Pro Gly Glu Lys Pro Tyr Lys Cys Pro 
1               5                   10                  15      
Glu Cys Gly Lys Ser Phe Ser Glu Arg Ser His Leu Arg Glu His Gln 
            20                  25                  30          
Arg Thr His Thr Gly Glu Lys Pro Tyr Lys Cys Pro Glu Cys Gly Lys 
        35                  40                  45              
Ser Phe Ser Arg Ala Asp Asn Leu Thr Glu His Gln Arg Thr His Thr 
    50                  55                  60                  
Gly Glu Lys Pro Tyr Lys Cys Pro Glu Cys Gly Lys Ser Phe Ser Ser 
65                  70                  75                  80  
Arg Arg Thr Cys Arg Ala His Gln Arg Thr His Thr Gly Glu Lys Pro 
                85                  90                  95      
Tyr Lys Cys Pro Glu Cys Gly Lys Ser Phe Ser Arg Asn Asp Thr Leu 
            100                 105                 110         
Thr Glu His Gln Arg Thr His Thr Gly Glu Lys Pro Tyr Lys Cys Pro 
        115                 120                 125             
Glu Cys Gly Lys Ser Phe Ser Arg Ser Asp Lys Leu Thr Glu His Gln 
    130                 135                 140                 
Arg Thr His Thr Gly Glu Lys Pro Tyr Lys Cys Pro Glu Cys Gly Lys 
145                 150                 155                 160 
Ser Phe Ser Gln Leu Ala His Leu Arg Ala His Gln Arg Thr His Thr 
                165                 170                 175     



<210> 34
<211> 555
<212> DNA
<213> Artificial Sequence


<220> 
<223> ZNF-E2C Nucleotide

<400> 34
atggcgcagg cggcgctgga accgggcgaa aaaccgtata aatgcccgga atgcggcaaa      60

agctttagcc gcaaagatag cctggtgcgc catcagcgca cccataccgg cgaaaaaccg     120

tataaatgcc cggaatgcgg caaaagcttt agccagagcg gcgatctgcg ccgccatcag     180

cgcacccata ccggcgaaaa accgtataaa tgcccggaat gcggcaaaag ctttagcgat     240

tgccgcgatc tggcgcgcca tcagcgcacc cataccggcg aaaaaccgta taaatgcccg     300

gaatgcggca aaagctttag ccagagcagc catctggtgc gccatcagcg cacccatacc     360

ggcgaaaaac cgtataaatg cccggaatgc ggcaaaagct ttagcgattg ccgcgatctg     420

gcgcgccatc agcgcaccca taccggcgaa aaaccgtata aatgcccgga atgcggcaaa     480

agctttagcc gcagcgataa actggtgcgc catcagcgca cccataccgg caaaaaaacc     540

agcggccagg cgggc                                                      555


<210> 35
<211> 185
<212> PRT
<213> Artificial Sequence


<220> 
<223> ZNF-E2C Aminoacid

<400> 35
Met Ala Gln Ala Ala Leu Glu Pro Gly Glu Lys Pro Tyr Lys Cys Pro 
1               5                   10                  15      
Glu Cys Gly Lys Ser Phe Ser Arg Lys Asp Ser Leu Val Arg His Gln 
            20                  25                  30          
Arg Thr His Thr Gly Glu Lys Pro Tyr Lys Cys Pro Glu Cys Gly Lys 
        35                  40                  45              
Ser Phe Ser Gln Ser Gly Asp Leu Arg Arg His Gln Arg Thr His Thr 
    50                  55                  60                  
Gly Glu Lys Pro Tyr Lys Cys Pro Glu Cys Gly Lys Ser Phe Ser Asp 
65                  70                  75                  80  
Cys Arg Asp Leu Ala Arg His Gln Arg Thr His Thr Gly Glu Lys Pro 
                85                  90                  95      
Tyr Lys Cys Pro Glu Cys Gly Lys Ser Phe Ser Gln Ser Ser His Leu 
            100                 105                 110         
Val Arg His Gln Arg Thr His Thr Gly Glu Lys Pro Tyr Lys Cys Pro 
        115                 120                 125             
Glu Cys Gly Lys Ser Phe Ser Asp Cys Arg Asp Leu Ala Arg His Gln 
    130                 135                 140                 
Arg Thr His Thr Gly Glu Lys Pro Tyr Lys Cys Pro Glu Cys Gly Lys 
145                 150                 155                 160 
Ser Phe Ser Arg Ser Asp Lys Leu Val Arg His Gln Arg Thr His Thr 
                165                 170                 175     
Gly Lys Lys Thr Ser Gly Gln Ala Gly 
            180                 185 

<210> 36
<211> 555
<212> DNA
<213> Artificial Sequence


<220> 
<223> ZNF-E3 Nucleotide

<400> 36
atggcgcagg cggcgctgga accgggcgaa aaaccgtata aatgcccgga atgcggcaaa      60

agctttagcg atccgggcgc gctggtgcgc catcagcgca cccataccgg cgaaaaaccg     120

tataaatgcc cggaatgcgg caaaagcttt agccagagca gccatctggt gcgccatcag     180

cgcacccata ccggcgaaaa accgtataaa tgcccggaat gcggcaaaag ctttagcgat     240

tgccgcgatc tggcgcgcca tcagcgcacc cataccggcg aaaaaccgta taaatgcccg     300

gaatgcggca aaagctttag ccagagcagc catctggtgc gccatcagcg cacccatacc     360

ggcgaaaaac cgtataaatg cccggaatgc ggcaaaagct ttagcgattg ccgcgatctg     420

gcgcgccatc agcgcaccca taccggcgaa aaaccgtata aatgcccgga atgcggcaaa     480

agctttagcc agagcagcca tctggtgcgc catcagcgca cccataccgg caaaaaaacc     540

agcggccagg cgggc                                                      555


<210> 37
<211> 185
<212> PRT
<213> Artificial Sequence


<220> 
<223> ZNF-E3 Amino acid

<400> 37
Met Ala Gln Ala Ala Leu Glu Pro Gly Glu Lys Pro Tyr Lys Cys Pro 
1               5                   10                  15      
Glu Cys Gly Lys Ser Phe Ser Asp Pro Gly Ala Leu Val Arg His Gln 
            20                  25                  30          
Arg Thr His Thr Gly Glu Lys Pro Tyr Lys Cys Pro Glu Cys Gly Lys 
        35                  40                  45              
Ser Phe Ser Gln Ser Ser His Leu Val Arg His Gln Arg Thr His Thr 
    50                  55                  60                  
Gly Glu Lys Pro Tyr Lys Cys Pro Glu Cys Gly Lys Ser Phe Ser Asp 
65                  70                  75                  80  
Cys Arg Asp Leu Ala Arg His Gln Arg Thr His Thr Gly Glu Lys Pro 
                85                  90                  95      
Tyr Lys Cys Pro Glu Cys Gly Lys Ser Phe Ser Gln Ser Ser His Leu 
            100                 105                 110         
Val Arg His Gln Arg Thr His Thr Gly Glu Lys Pro Tyr Lys Cys Pro 
        115                 120                 125             
Glu Cys Gly Lys Ser Phe Ser Asp Cys Arg Asp Leu Ala Arg His Gln 
    130                 135                 140                 
Arg Thr His Thr Gly Glu Lys Pro Tyr Lys Cys Pro Glu Cys Gly Lys 
145                 150                 155                 160 
Ser Phe Ser Gln Ser Ser His Leu Val Arg His Gln Arg Thr His Thr 
                165                 170                 175     
Gly Lys Lys Thr Ser Gly Gln Ala Gly 
            180                 185 

<210> 38
<211> 528
<212> DNA
<213> Artificial sequence


<220> 
<223> ZNF-TRCa Nucleotide

<400> 38
atggcgcagg cggctcttga acccggggag aaaccctata aatgccctga gtgtggcaag      60

agtttttcaa ccacaggaaa cttgacagtc caccaacgga cccacaccgg cgagaaacca     120

tacaagtgtc cggagtgtgg taagtctttc tcaagtcctg ccgaccttac cagacatcaa     180

cgcacacata caggtgaaaa accttacaag tgcccagagt gcggaaaaag tttttcacaa     240

tctggcgacc tccgcaggca ccagcgcact cacaccggtg aaaaaccata caagtgtcct     300

gagtgcggga agagttttag tcaacgagct catctggagc gacaccaaag gactcatact     360

ggggagaaac cgtacaaatg tcccgaatgt gggaagagct tctctaccaa gaattccctt     420

acagagcacc agcgcacgca tacgggagag aagccgtata agtgtccgga atgtggcaag     480

agcttttcca gaagtgacca ccttacaacc caccagagga cgcacacc                  528


<210> 39
<211> 176
<212> PRT
<213> Artificial Sequence


<220> 
<223> ZNF-TRCa Aminoacid

<400> 39
Met Ala Gln Ala Ala Leu Glu Pro Gly Glu Lys Pro Tyr Lys Cys Pro 
1               5                   10                  15      
Glu Cys Gly Lys Ser Phe Ser Thr Thr Gly Asn Leu Thr Val His Gln 
            20                  25                  30          
Arg Thr His Thr Gly Glu Lys Pro Tyr Lys Cys Pro Glu Cys Gly Lys 
        35                  40                  45              
Ser Phe Ser Ser Pro Ala Asp Leu Thr Arg His Gln Arg Thr His Thr 
    50                  55                  60                  
Gly Glu Lys Pro Tyr Lys Cys Pro Glu Cys Gly Lys Ser Phe Ser Gln 
65                  70                  75                  80  
Ser Gly Asp Leu Arg Arg His Gln Arg Thr His Thr Gly Glu Lys Pro 
                85                  90                  95      
Tyr Lys Cys Pro Glu Cys Gly Lys Ser Phe Ser Gln Arg Ala His Leu 
            100                 105                 110         
Glu Arg His Gln Arg Thr His Thr Gly Glu Lys Pro Tyr Lys Cys Pro 
        115                 120                 125             
Glu Cys Gly Lys Ser Phe Ser Thr Lys Asn Ser Leu Thr Glu His Gln 
    130                 135                 140                 
Arg Thr His Thr Gly Glu Lys Pro Tyr Lys Cys Pro Glu Cys Gly Lys 
145                 150                 155                 160 
Ser Phe Ser Arg Ser Asp His Leu Thr Thr His Gln Arg Thr His Thr 
                165                 170                 175     



<210> 40
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> GGS linker Nucleic Acid

<400> 40
ggtggatctg gcggtggatc tggtggcggt                                     30


<210> 41
<211> 10
<212> PRT
<213> Artificial Sequence


<220> 
<223> GGS linker Amino Acid

<400> 41
Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly 
1               5                   10  

<210> 42
<211> 36
<212> DNA
<213> Artificial Sequence


<220> 
<223> GGS4x Linker Nucleic Acid

<400> 42
ggagggagtg gtgggtccgg tggtagtggc ggatcc                               36


<210> 43
<211> 12
<212> PRT
<213> Artificial Sequence


<220> 
<223> GGS4x Linker Amino Acid

<400> 43
Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser 
1               5                   10          

<210> 44
<211> 45
<212> DNA
<213> Artificial Sequence


<220> 
<223> GGS5x Linker Nucleic Acid

<400> 44
ggaggctccg gtgggtctgg tgggagcggt ggtagtggcg gatcc                     45


<210> 45
<211> 15
<212> PRT
<213> Artificial Sequence


<220> 
<223> GGS5x Linker Amino Acid

<400> 45
Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser 
1               5                   10                  15  

<210> 46
<211> 54
<212> DNA
<213> Artificial Sequence


<220> 
<223> GGS6x Linker Nucleic Acid

<400> 46
ggaggcagtg gtgggagcgg tggttccggg ggtagtggtg gttccggggg atcc           54


<210> 47
<211> 18
<212> PRT
<213> Artificial Sequence


<220> 
<223> GGS6x Linker Amino Acid

<400> 47
Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
1               5                   10                  15      
Gly Ser 
        

<210> 48
<211> 63
<212> DNA
<213> Artificial Sequence


<220> 
<223> GGS7x Linker Nucleic Acid

<400> 48
ggaggttctg gaggctccgg tgggtccggg ggaagtgggg ggtcaggcgg atcaggagga     60

tcc                                                                   63


<210> 49
<211> 21
<212> PRT
<213> Artificial Sequence


<220> 
<223> GGS7x Linker Amino Acid

<400> 49
Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
1               5                   10                  15      
Gly Ser Gly Gly Ser 
            20      

<210> 50
<211> 75
<212> DNA
<213> Artificial Sequence


<220> 
<223> GGS8x Linker Nucleic Acid

<400> 50
ggaggtagcg gaggttccgg agggagcggc gggagtgggg gaagcggggg aagtggagga     60

tccgggggag gatcc                                                      75


<210> 51
<211> 21
<212> PRT
<213> Artificial Sequence


<220> 
<223> GGS8x Linker Amino Acid

<400> 51
Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
1               5                   10                  15      
Gly Ser Gly Gly Ser 
            20      

<210> 52
<211> 48
<212> DNA
<213> Artificial Sequence


<220> 
<223> Linker XTEN Nucleic Acid

<400> 52
tccggtagcg aaacaccggg gacttcagaa tcggccaccc cggagtct                  48


<210> 53
<211> 16
<212> PRT
<213> Artificial Sequence


<220> 
<223> Linker XTEN Amino Acid

<400> 53
Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser 
1               5                   10                  15      



<210> 54
<211> 36
<212> DNA
<213> Artificial Sequence


<220> 
<223> Linker B Nucleic Acid

<400> 54
ggaagcgccg gtagtgcggc tgggtctggc gagttc                               36


<210> 55
<211> 12
<212> PRT
<213> Artificial Sequence


<220> 
<223> Linker B Amino Acid

<400> 55
Gly Ser Ala Gly Ser Ala Ala Gly Ser Gly Glu Phe 
1               5                   10          

<210> 56
<211> 4104
<212> DNA
<213> Artificial Sequence


<220> 
<223> human Cas9 (hCas9) nucleic acid

<400> 56
atggacaaga agtactccat tgggctcgat atcggcacaa acagcgtcgg ctgggccgtc       60

attacggacg agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa taccgatcgc      120

cacagcataa agaagaacct cattggcgcc ctcctgttcg actccgggga gacggccgaa      180

gccacgcggc tcaaaagaac agcacggcgc agatataccc gcagaaagaa tcggatctgc      240

tacctgcagg agatctttag taatgagatg gctaaggtgg atgactcttt cttccatagg      300

ctggaggagt cctttttggt ggaggaggat aaaaagcacg agcgccaccc aatctttggc      360

aatatcgtgg acgaggtggc gtaccatgaa aagtacccaa ccatatatca tctgaggaag      420

aagcttgtag acagtactga taaggctgac ttgcggttga tctatctcgc gctggcgcat      480

atgatcaaat ttcggggaca cttcctcatc gagggggacc tgaacccaga caacagcgat      540

gtcgacaaac tctttatcca actggttcag acttacaatc agcttttcga agagaacccg      600

atcaacgcat ccggagttga cgccaaagca atcctgagcg ctaggctgtc caaatcccgg      660

cggctcgaaa acctcatcgc acagctccct ggggagaaga agaacggcct gtttggtaat      720

cttatcgccc tgtcactcgg gctgaccccc aactttaaat ctaacttcga cctggccgaa      780

gatgccaagc ttcaactgag caaagacacc tacgatgatg atctcgacaa tctgctggcc      840

cagatcggcg accagtacgc agaccttttt ttggcggcaa agaacctgtc agacgccatt      900

ctgctgagtg atattctgcg agtgaacacg gagatcacca aagctccgct gagcgctagt      960

atgatcaagc gctatgatga gcaccaccaa gacttgactt tgctgaaggc ccttgtcaga     1020

cagcaactgc ctgagaagta caaggaaatt ttcttcgatc agtctaaaaa tggctacgcc     1080

ggatacattg acggcggagc aagccaggag gaattttaca aatttattaa gcccatcttg     1140

gaaaaaatgg acggcaccga ggagctgctg gtaaagctta acagagaaga tctgttgcgc     1200

aaacagcgca ctttcgacaa tggaagcatc ccccaccaga ttcacctggg cgaactgcac     1260

gctatcctca ggcggcaaga ggatttctac ccctttttga aagataacag ggaaaagatt     1320

gagaaaatcc tcacatttcg gataccctac tatgtaggcc ccctcgcccg gggaaattcc     1380

agattcgcgt ggatgactcg caaatcagaa gagaccatca ctccctggaa cttcgaggaa     1440

gtcgtggata agggggcctc tgcccagtcc ttcatcgaaa ggatgactaa ctttgataaa     1500

aatctgccta acgaaaaggt gcttcctaaa cactctctgc tgtacgagta cttcacagtt     1560

tataacgagc tcaccaaggt caaatacgtc acagaaggga tgagaaagcc agcattcctg     1620

tctggagagc agaagaaagc tatcgtggac ctcctcttca agacgaaccg gaaagttacc     1680

gtgaaacagc tcaaagaaga ctatttcaaa aagattgaat gtttcgactc tgttgaaatc     1740

agcggagtgg aggatcgctt caacgcatcc ctgggaacgt atcacgatct cctgaaaatc     1800

attaaagaca aggacttcct ggacaatgag gagaacgagg acattcttga ggacattgtc     1860

ctcaccctta cgttgtttga agatagggag atgattgaag aacgcttgaa aacttacgct     1920

catctcttcg acgacaaagt catgaaacag ctcaagaggc gccgatatac aggatggggg     1980

cggctgtcaa gaaaactgat caatgggatc cgagacaagc agagtggaaa gacaatcctg     2040

gattttctta agtccgatgg atttgccaac cggaacttca tgcagttgat ccatgatgac     2100

tctctcacct ttaaggagga catccagaaa gcacaagttt ctggccaggg ggacagtctt     2160

cacgagcaca tcgctaatct tgcaggtagc ccagctatca aaaagggaat actgcagacc     2220

gttaaggtcg tggatgaact cgtcaaagta atgggaaggc ataagcccga gaatatcgtt     2280

atcgagatgg cccgagagaa ccaaactacc cagaagggac agaagaacag tagggaaagg     2340

atgaagagga ttgaagaggg tataaaagaa ctggggtccc aaatccttaa ggaacaccca     2400

gttgaaaaca cccagcttca gaatgagaag ctctacctgt actacctgca gaacggcagg     2460

gacatgtacg tggatcagga actggacatc aatcggctct ccgactacga cgtggatcat     2520

atcgtgcccc agtcttttct caaagatgat tctattgata ataaagtgtt gacaagatcc     2580

gataaaaata gagggaagag tgataacgtc ccctcagaag aagttgtcaa gaaaatgaaa     2640

aattattggc ggcagctgct gaacgccaaa ctgatcacac aacggaagtt cgataatctg     2700

actaaggctg aacgaggtgg cctgtctgag ttggataaag ccggcttcat caaaaggcag     2760

cttgttgaga cacgccagat caccaagcac gtggcccaaa ttctcgattc acgcatgaac     2820

accaagtacg atgaaaatga caaactgatt cgagaggtga aagttattac tctgaagtct     2880

aagctggtct cagatttcag aaaggacttt cagttttata aggtgagaga gatcaacaat     2940

taccaccatg cgcatgatgc ctacctgaat gcagtggtag gcactgcact tatcaaaaaa     3000

tatcccaagc ttgaatctga atttgtttac ggagactata aagtgtacga tgttaggaaa     3060

atgatcgcaa agtctgagca ggaaataggc aaggccaccg ctaagtactt cttttacagc     3120

aatattatga attttttcaa gaccgagatt acactggcca atggagagat tcggaagcga     3180

ccacttatcg aaacaaacgg agaaacagga gaaatcgtgt gggacaaggg tagggatttc     3240

gcgacagtcc ggaaggtcct gtccatgccg caggtgaaca tcgttaaaaa gaccgaagta     3300

cagaccggag gcttctccaa ggaaagtatc ctcccgaaaa ggaacagcga caagctgatc     3360

gcacgcaaaa aagattggga ccccaagaaa tacggcggat tcgattctcc tacagtcgct     3420

tacagtgtac tggttgtggc caaagtggag aaagggaagt ctaaaaaact caaaagcgtc     3480

aaggaactgc tgggcatcac aatcatggag cgatcaagct tcgaaaaaaa ccccatcgac     3540

tttctcgagg cgaaaggata taaagaggtc aaaaaagacc tcatcattaa gcttcccaag     3600

tactctctct ttgagcttga aaacggccgg aaacgaatgc tcgctagtgc gggcgagctg     3660

cagaaaggta acgagctggc actgccctct aaatacgtta atttcttgta tctggccagc     3720

cactatgaaa agctcaaagg gtctcccgaa gataatgagc agaagcagct gttcgtggaa     3780

caacacaaac actaccttga tgagatcatc gagcaaataa gcgaattctc caaaagagtg     3840

atcctcgccg acgctaacct cgataaggtg ctttctgctt acaataagca cagggataag     3900

cccatcaggg agcaggcaga aaacattatc cacttgttta ctctgaccaa cttgggcgcg     3960

cctgcagcct tcaagtactt cgacaccacc atagacagaa agcggtacac ctctacaaag     4020

gaggtcctgg acgccacact gattcatcag tcaattacgg ggctctatga aacaagaatc     4080

gacctctctc agctcggtgg agac                                            4104


<210> 57
<211> 4104
<212> DNA
<213> Artificial Sequence


<220> 
<223> nickase Cas9 (nCas9) nucleic acid

<400> 57
atggacaaga agtactccat tgggctcgct atcggcacaa acagcgtcgg ctgggccgtc       60

attacggacg agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa taccgatcgc      120

cacagcataa agaagaacct cattggcgcc ctcctgttcg actccgggga gacggccgaa      180

gccacgcggc tcaaaagaac agcacggcgc agatataccc gcagaaagaa tcggatctgc      240

tacctgcagg agatctttag taatgagatg gctaaggtgg atgactcttt cttccatagg      300

ctggaggagt cctttttggt ggaggaggat aaaaagcacg agcgccaccc aatctttggc      360

aatatcgtgg acgaggtggc gtaccatgaa aagtacccaa ccatatatca tctgaggaag      420

aagcttgtag acagtactga taaggctgac ttgcggttga tctatctcgc gctggcgcat      480

atgatcaaat ttcggggaca cttcctcatc gagggggacc tgaacccaga caacagcgat      540

gtcgacaaac tctttatcca actggttcag acttacaatc agcttttcga agagaacccg      600

atcaacgcat ccggagttga cgccaaagca atcctgagcg ctaggctgtc caaatcccgg      660

cggctcgaaa acctcatcgc acagctccct ggggagaaga agaacggcct gtttggtaat      720

cttatcgccc tgtcactcgg gctgaccccc aactttaaat ctaacttcga cctggccgaa      780

gatgccaagc ttcaactgag caaagacacc tacgatgatg atctcgacaa tctgctggcc      840

cagatcggcg accagtacgc agaccttttt ttggcggcaa agaacctgtc agacgccatt      900

ctgctgagtg atattctgcg agtgaacacg gagatcacca aagctccgct gagcgctagt      960

atgatcaagc gctatgatga gcaccaccaa gacttgactt tgctgaaggc ccttgtcaga     1020

cagcaactgc ctgagaagta caaggaaatt ttcttcgatc agtctaaaaa tggctacgcc     1080

ggatacattg acggcggagc aagccaggag gaattttaca aatttattaa gcccatcttg     1140

gaaaaaatgg acggcaccga ggagctgctg gtaaagctta acagagaaga tctgttgcgc     1200

aaacagcgca ctttcgacaa tggaagcatc ccccaccaga ttcacctggg cgaactgcac     1260

gctatcctca ggcggcaaga ggatttctac ccctttttga aagataacag ggaaaagatt     1320

gagaaaatcc tcacatttcg gataccctac tatgtaggcc ccctcgcccg gggaaattcc     1380

agattcgcgt ggatgactcg caaatcagaa gagaccatca ctccctggaa cttcgaggaa     1440

gtcgtggata agggggcctc tgcccagtcc ttcatcgaaa ggatgactaa ctttgataaa     1500

aatctgccta acgaaaaggt gcttcctaaa cactctctgc tgtacgagta cttcacagtt     1560

tataacgagc tcaccaaggt caaatacgtc acagaaggga tgagaaagcc agcattcctg     1620

tctggagagc agaagaaagc tatcgtggac ctcctcttca agacgaaccg gaaagttacc     1680

gtgaaacagc tcaaagaaga ctatttcaaa aagattgaat gtttcgactc tgttgaaatc     1740

agcggagtgg aggatcgctt caacgcatcc ctgggaacgt atcacgatct cctgaaaatc     1800

attaaagaca aggacttcct ggacaatgag gagaacgagg acattcttga ggacattgtc     1860

ctcaccctta cgttgtttga agatagggag atgattgaag aacgcttgaa aacttacgct     1920

catctcttcg acgacaaagt catgaaacag ctcaagaggc gccgatatac aggatggggg     1980

cggctgtcaa gaaaactgat caatgggatc cgagacaagc agagtggaaa gacaatcctg     2040

gattttctta agtccgatgg atttgccaac aggaacttca tgcagttgat ccatgatgac     2100

tctctcacct ttaaggagga catccagaaa gcacaagttt ctggccaggg ggacagtctt     2160

cacgagcaca tcgctaatct tgcaggtagc ccagctatca aaaagggaat actgcagacc     2220

gttaaggtcg tggatgaact cgtcaaagta atgggaaggc ataagcccga gaatatcgtt     2280

atcgagatgg cccgagagaa ccaaactacc cagaagggac agaagaacag tagggaaagg     2340

atgaagagga ttgaagaggg tataaaagaa ctggggtccc aaatccttaa ggaacaccca     2400

gttgaaaaca cccagcttca gaatgagaag ctctacctgt actacctgca gaacggcagg     2460

gacatgtacg tggatcagga actggacatc aatcggctct ccgactacga cgtggatcat     2520

atcgtgcccc agtcttttct caaagatgat tctattgata ataaagtgtt gacaagatcc     2580

gataaaaata gagggaagag tgataacgtc ccctcagaag aagttgtcaa gaaaatgaaa     2640

aattattggc ggcagctgct gaacgccaaa ctgatcacac aacggaagtt cgataatctg     2700

actaaggctg aacgaggtgg cctgtctgag ttggataaag ccggcttcat caaaaggcag     2760

cttgttgaga cacgccagat caccaagcac gtggcccaaa ttctcgattc acgcatgaac     2820

accaagtacg atgaaaatga caaactgatt cgagaggtga aagttattac tctgaagtct     2880

aagctggtct cagatttcag aaaggacttt cagttttata aggtgagaga gatcaacaat     2940

taccaccatg cgcatgatgc ctacctgaat gcagtggtag gcactgcact tatcaaaaaa     3000

tatcccaagc ttgaatctga atttgtttac ggagactata aagtgtacga tgttaggaaa     3060

atgatcgcaa agtctgagca ggaaataggc aaggccaccg ctaagtactt cttttacagc     3120

aatattatga attttttcaa gaccgagatt acactggcca atggagagat tcggaagcga     3180

ccacttatcg aaacaaacgg agaaacagga gaaatcgtgt gggacaaggg tagggatttc     3240

gcgacagtcc ggaaggtcct gtccatgccg caggtgaaca tcgttaaaaa gaccgaagta     3300

cagaccggag gcttctccaa ggaaagtatc ctcccgaaaa ggaacagcga caagctgatc     3360

gcacgcaaaa aagattggga ccccaagaaa tacggcggat tcgattctcc tacagtcgct     3420

tacagtgtac tggttgtggc caaagtggag aaagggaagt ctaaaaaact caaaagcgtc     3480

aaggaactgc tgggcatcac aatcatggag cgatcaagct tcgaaaaaaa ccccatcgac     3540

tttctcgagg cgaaaggata taaagaggtc aaaaaagacc tcatcattaa gcttcccaag     3600

tactctctct ttgagcttga aaacggccgg aaacgaatgc tcgctagtgc gggcgagctg     3660

cagaaaggta acgagctggc actgccctct aaatacgtta atttcttgta tctggccagc     3720

cactatgaaa agctcaaagg gtctcccgaa gataatgagc agaagcagct gttcgtggaa     3780

caacacaaac actaccttga tgagatcatc gagcaaataa gcgaattctc caaaagagtg     3840

atcctcgccg acgctaacct cgataaggtg ctttctgctt acaataagca cagggataag     3900

cccatcaggg agcaggcaga aaacattatc cacttgttta ctctgaccaa cttgggcgcg     3960

cctgcagcct tcaagtactt cgacaccacc atagacagaa agcggtacac ctctacaaag     4020

gaggtcctgg acgccacact gattcatcag tcaattacgg ggctctatga aacaagaatc     4080

gacctctctc agctcggtgg agac                                            4104


<210> 58
<211> 3213
<212> DNA
<213> Staphylococcus aureus


<400> 58
gccaccatgg ccccaaagaa gaagcggaag gtcggtatcc acggagtccc agcagccaag       60

cggaactaca tcctgggcct ggccatcggc atcaccagcg tgggctacgg catcatcgac      120

tacgagacac gggacgtgat cgatgccggc gtgcggctgt tcaaagaggc caacgtggaa      180

aacaacgagg gcaggcggag caagagaggc gccagaaggc tgaagcggcg gaggcggcat      240

agaatccaga gagtgaagaa gctgctgttc gactacaacc tgctgaccga ccacagcgag      300

ctgagcggca tcaaccccta cgaggccaga gtgaagggcc tgagccagaa gctgagcgag      360

gaagagttct ctgccgccct gctgcacctg gccaagagaa gaggcgtgca caacgtgaac      420

gaggtggaag aggacaccgg caacgagctg tccaccaaag agcagatcag ccggaacagc      480

aaggccctgg aagagaaata cgtggccgaa ctgcagctgg aacggctgaa gaaagacggc      540

gaagtgcggg gcagcatcaa cagattcaag accagcgact acgtgaaaga agccaaacag      600

ctgctgaagg tgcagaaggc ctaccaccag ctggaccaga gcttcatcga cacctacatc      660

gacctgctgg aaacccggcg gacctactat gagggacctg gcgagggcag ccccttcggc      720

tggaaggaca tcaaagaatg gtacgagatg ctgatgggcc actgcaccta cttccccgag      780

gaactgcgga gcgtgaagta cgcctacaac gccgacctgt acaacgccct gaacgacctg      840

aacaatctcg tgatcaccag ggacgagaac gagaagctgg aatattacga gaagttccag      900

atcatcgaga acgtgttcaa gcagaagaag aagcccaccc tgaagcagat cgccaaagaa      960

atcctcgtga acgaagagga tattaagggc tacagagtga ccagcaccgg caagcccgag     1020

ttcaccaacc tgaaggtgta ccacgacatc aaggacatta ccgcccggaa agagattatt     1080

gagaacgccg agctgctgga tcagattgcc aagatcctga ccatctacca gagcagcgag     1140

gacatccagg aagaactgac caatctgaac tccgagctga cccaggaaga gatcgagcag     1200

atctctaatc tgaagggcta taccggcacc cacaacctga gcctgaaggc catcaacctg     1260

atcctggacg agctgtggca caccaacgac aaccagatcg ctatcttcaa ccggctgaag     1320

ctggtgccca agaaggtgga cctgtcccag cagaaagaga tccccaccac cctggtggac     1380

gacttcatcc tgagccccgt cgtgaagaga agcttcatcc agagcatcaa agtgatcaac     1440

gccatcatca agaagtacgg cctgcccaac gacatcatta tcgagctggc ccgcgagaag     1500

aactccaagg acgcccagaa aatgatcaac gagatgcaga agcggaaccg gcagaccaac     1560

gagcggatcg aggaaatcat ccggaccacc ggcaaagaga acgccaagta cctgatcgag     1620

aagatcaagc tgcacgacat gcaggaaggc aagtgcctgt acagcctgga agccatccct     1680

ctggaagatc tgctgaacaa ccccttcaac tatgaggtgg accacatcat ccccagaagc     1740

gtgtccttcg acaacagctt caacaacaag gtgctcgtga agcaggaaga aaacagcaag     1800

aagggcaacc ggaccccatt ccagtacctg agcagcagcg acagcaagat cagctacgaa     1860

accttcaaga agcacatcct gaatctggcc aagggcaagg gcagaatcag caagaccaag     1920

aaagagtatc tgctggaaga acgggacatc aacaggttct ccgtgcagaa agacttcatc     1980

aaccggaacc tggtggatac cagatacgcc accagaggcc tgatgaacct gctgcggagc     2040

tacttcagag tgaacaacct ggacgtgaaa gtgaagtcca tcaatggcgg cttcaccagc     2100

tttctgcggc ggaagtggaa gtttaagaaa gagcggaaca aggggtacaa gcaccacgcc     2160

gaggacgccc tgatcattgc caacgccgat ttcatcttca aagagtggaa gaaactggac     2220

aaggccaaaa aagtgatgga aaaccagatg ttcgaggaaa agcaggccga gagcatgccc     2280

gagatcgaaa ccgagcagga gtacaaagag atcttcatca ccccccacca gatcaagcac     2340

attaaggact tcaaggacta caagtacagc caccgggtgg acaagaagcc taatagagag     2400

ctgattaacg acaccctgta ctccacccgg aaggacgaca agggcaacac cctgatcgtg     2460

aacaatctga acggcctgta cgacaaggac aatgacaagc tgaaaaagct gatcaacaag     2520

agccccgaaa agctgctgat gtaccaccac gacccccaga cctaccagaa actgaagctg     2580

attatggaac agtacggcga cgagaagaat cccctgtaca agtactacga ggaaaccggg     2640

aactacctga ccaagtactc caaaaaggac aacggccccg tgatcaagaa gattaagtat     2700

tacggcaaca aactgaacgc ccatctggac atcaccgacg actaccccaa cagcagaaac     2760

aaggtcgtga agctgtccct gaagccctac agattcgacg tgtacctgga caatggcgtg     2820

tacaagttcg tgaccgtgaa gaatctggat gtgatcaaaa aagaaaacta ctacgaagtg     2880

aatagcaagt gctatgagga agctaagaag ctgaagaaga tcagcaacca ggccgagttt     2940

atcgcctcct tctacaacaa cgatctgatc aagatcaacg gcgagctgta tagagtgatc     3000

ggcgtgaaca acgacctgct gaaccggatc gaagtgaaca tgatcgacat cacctaccgc     3060

gagtacctgg aaaacatgaa cgacaagagg ccccccagga tcattaagac aatcgcctcc     3120

aagacccaga gcattaagaa gtacagcaca gacattctgg gcaacctgta tgaagtgaaa     3180

tctaagaagc accctcagat catcaaaaag ggc                                  3213


<210> 59
<211> 4104
<212> DNA
<213> Artificial Sequence


<220> 
<223> dead Cas9 (dCas9) nucleic acid

<400> 59
atggacaaga agtactccat tgggctcgct atcggcacaa acagcgtcgg ctgggccgtc       60

attacggacg agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa taccgatcgc      120

cacagcataa agaagaacct cattggcgcc ctcctgttcg actccgggga gacggccgaa      180

gccacgcggc tcaaaagaac agcacggcgc agatataccc gcagaaagaa tcggatctgc      240

tacctgcagg agatctttag taatgagatg gctaaggtgg atgactcttt cttccatagg      300

ctggaggagt cctttttggt ggaggaggat aaaaagcacg agcgccaccc aatctttggc      360

aatatcgtgg acgaggtggc gtaccatgaa aagtacccaa ccatatatca tctgaggaag      420

aagcttgtag acagtactga taaggctgac ttgcggttga tctatctcgc gctggcgcat      480

atgatcaaat ttcggggaca cttcctcatc gagggggacc tgaacccaga caacagcgat      540

gtcgacaaac tctttatcca actggttcag acttacaatc agcttttcga agagaacccg      600

atcaacgcat ccggagttga cgccaaagca atcctgagcg ctaggctgtc caaatcccgg      660

cggctcgaaa acctcatcgc acagctccct ggggagaaga agaacggcct gtttggtaat      720

cttatcgccc tgtcactcgg gctgaccccc aactttaaat ctaacttcga cctggccgaa      780

gatgccaagc ttcaactgag caaagacacc tacgatgatg atctcgacaa tctgctggcc      840

cagatcggcg accagtacgc agaccttttt ttggcggcaa agaacctgtc agacgccatt      900

ctgctgagtg atattctgcg agtgaacacg gagatcacca aagctccgct gagcgctagt      960

atgatcaagc gctatgatga gcaccaccaa gacttgactt tgctgaaggc ccttgtcaga     1020

cagcaactgc ctgagaagta caaggaaatt ttcttcgatc agtctaaaaa tggctacgcc     1080

ggatacattg acggcggagc aagccaggag gaattttaca aatttattaa gcccatcttg     1140

gaaaaaatgg acggcaccga ggagctgctg gtaaagctta acagagaaga tctgttgcgc     1200

aaacagcgca ctttcgacaa tggaagcatc ccccaccaga ttcacctggg cgaactgcac     1260

gctatcctca ggcggcaaga ggatttctac ccctttttga aagataacag ggaaaagatt     1320

gagaaaatcc tcacatttcg gataccctac tatgtaggcc ccctcgcccg gggaaattcc     1380

agattcgcgt ggatgactcg caaatcagaa gagaccatca ctccctggaa cttcgaggaa     1440

gtcgtggata agggggcctc tgcccagtcc ttcatcgaaa ggatgactaa ctttgataaa     1500

aatctgccta acgaaaaggt gcttcctaaa cactctctgc tgtacgagta cttcacagtt     1560

tataacgagc tcaccaaggt caaatacgtc acagaaggga tgagaaagcc agcattcctg     1620

tctggagagc agaagaaagc tatcgtggac ctcctcttca agacgaaccg gaaagttacc     1680

gtgaaacagc tcaaagaaga ctatttcaaa aagattgaat gtttcgactc tgttgaaatc     1740

agcggagtgg aggatcgctt caacgcatcc ctgggaacgt atcacgatct cctgaaaatc     1800

attaaagaca aggacttcct ggacaatgag gagaacgagg acattcttga ggacattgtc     1860

ctcaccctta cgttgtttga agatagggag atgattgaag aacgcttgaa aacttacgct     1920

catctcttcg acgacaaagt catgaaacag ctcaagaggc gccgatatac aggatggggg     1980

cggctgtcaa gaaaactgat caatgggatc cgagacaagc agagtggaaa gacaatcctg     2040

gattttctta agtccgatgg atttgccaac cggaacttca tgcagttgat ccatgatgac     2100

tctctcacct ttaaggagga catccagaaa gcacaagttt ctggccaggg ggacagtctt     2160

cacgagcaca tcgctaatct tgcaggtagc ccagctatca aaaagggaat actgcagacc     2220

gttaaggtcg tggatgaact cgtcaaagta atgggaaggc ataagcccga gaatatcgtt     2280

atcgagatgg cccgagagaa ccaaactacc cagaagggac agaagaacag tagggaaagg     2340

atgaagagga ttgaagaggg tataaaagaa ctggggtccc aaatccttaa ggaacaccca     2400

gttgaaaaca cccagcttca gaatgagaag ctctacctgt actacctgca gaacggcagg     2460

gacatgtacg tggatcagga actggacatc aatcggctct ccgactacga cgtggctgct     2520

atcgtgcccc agtcttttct caaagatgat tctattgata ataaagtgtt gacaagatcc     2580

gataaagcta gagggaagag tgataacgtc ccctcagaag aagttgtcaa gaaaatgaaa     2640

aattattggc ggcagctgct gaacgccaaa ctgatcacac aacggaagtt cgataatctg     2700

actaaggctg aacgaggtgg cctgtctgag ttggataaag ccggcttcat caaaaggcag     2760

cttgttgaga cacgccagat caccaagcac gtggcccaaa ttctcgattc acgcatgaac     2820

accaagtacg atgaaaatga caaactgatt cgagaggtga aagttattac tctgaagtct     2880

aagctggtct cagatttcag aaaggacttt cagttttata aggtgagaga gatcaacaat     2940

taccaccatg cgcatgatgc ctacctgaat gcagtggtag gcactgcact tatcaaaaaa     3000

tatcccaagc ttgaatctga atttgtttac ggagactata aagtgtacga tgttaggaaa     3060

atgatcgcaa agtctgagca ggaaataggc aaggccaccg ctaagtactt cttttacagc     3120

aatattatga attttttcaa gaccgagatt acactggcca atggagagat tcggaagcga     3180

ccacttatcg aaacaaacgg agaaacagga gaaatcgtgt gggacaaggg tagggatttc     3240

gcgacagtcc ggaaggtcct gtccatgccg caggtgaaca tcgttaaaaa gaccgaagta     3300

cagaccggag gcttctccaa ggaaagtatc ctcccgaaaa ggaacagcga caagctgatc     3360

gcacgcaaaa aagattggga ccccaagaaa tacggcggat tcgattctcc tacagtcgct     3420

tacagtgtac tggttgtggc caaagtggag aaagggaagt ctaaaaaact caaaagcgtc     3480

aaggaactgc tgggcatcac aatcatggag cgatcaagct tcgaaaaaaa ccccatcgac     3540

tttctcgagg cgaaaggata taaagaggtc aaaaaagacc tcatcattaa gcttcccaag     3600

tactctctct ttgagcttga aaacggccgg aaacgaatgc tcgctagtgc gggcgagctg     3660

cagaaaggta acgagctggc actgccctct aaatacgtta atttcttgta tctggccagc     3720

cactatgaaa agctcaaagg gtctcccgaa gataatgagc agaagcagct gttcgtggaa     3780

caacacaaac actaccttga tgagatcatc gagcaaataa gcgaattctc caaaagagtg     3840

atcctcgccg acgctaacct cgataaggtg ctttctgctt acaataagca cagggataag     3900

cccatcaggg agcaggcaga aaacattatc cacttgttta ctctgaccaa cttgggcgcg     3960

cctgcagcct tcaagtactt cgacaccacc atagacagaa agcggtacac ctctacaaag     4020

gaggtcctgg acgccacact gattcatcag tcaattacgg ggctctatga aacaagaatc     4080

gacctctctc agctcggtgg agac                                            4104


<210> 60
<211> 3207
<212> DNA
<213> Staphylococcus aureus


<400> 60
atggccccaa agaagaagcg gaaggtcggt atccacggag tcccagcagc caagcggaac       60

tacatcctgg gcctggacat cggcatcacc agcgtgggct acggcatcat cgactacgag      120

acacgggacg tgatcgatgc cggcgtgcgg ctgttcaaag aggccaacgt ggaaaacaac      180

gagggcaggc ggagcaagag aggcgccaga aggctgaagc ggcggaggcg gcatagaatc      240

cagagagtga agaagctgct gttcgactac aacctgctga ccgaccacag cgagctgagc      300

ggcatcaacc cctacgaggc cagagtgaag ggcctgagcc agaagctgag cgaggaagag      360

ttctctgccg ccctgctgca cctggccaag agaagaggcg tgcacaacgt gaacgaggtg      420

gaagaggaca ccggcaacga gctgtccacc aaagagcaga tcagccggaa cagcaaggcc      480

ctggaagaga aatacgtggc cgaactgcag ctggaacggc tgaagaaaga cggcgaagtg      540

cggggcagca tcaacagatt caagaccagc gactacgtga aagaagccaa acagctgctg      600

aaggtgcaga aggcctacca ccagctggac cagagcttca tcgacaccta catcgacctg      660

ctggaaaccc ggcggaccta ctatgaggga cctggcgagg gcagcccctt cggctggaag      720

gacatcaaag aatggtacga gatgctgatg ggccactgca cctacttccc cgaggaactg      780

cggagcgtga agtacgccta caacgccgac ctgtacaacg ccctgaacga cctgaacaat      840

ctcgtgatca ccagggacga gaacgagaag ctggaatatt acgagaagtt ccagatcatc      900

gagaacgtgt tcaagcagaa gaagaagccc accctgaagc agatcgccaa agaaatcctc      960

gtgaacgaag aggatattaa gggctacaga gtgaccagca ccggcaagcc cgagttcacc     1020

aacctgaagg tgtaccacga catcaaggac attaccgccc ggaaagagat tattgagaac     1080

gccgagctgc tggatcagat tgccaagatc ctgaccatct accagagcag cgaggacatc     1140

caggaagaac tgaccaatct gaactccgag ctgacccagg aagagatcga gcagatctct     1200

aatctgaagg gctataccgg cacccacaac ctgagcctga aggccatcaa cctgatcctg     1260

gacgagctgt ggcacaccaa cgacaaccag atcgctatct tcaaccggct gaagctggtg     1320

cccaagaagg tggacctgtc ccagcagaaa gagatcccca ccaccctggt ggacgacttc     1380

atcctgagcc ccgtcgtgaa gagaagcttc atccagagca tcaaagtgat caacgccatc     1440

atcaagaagt acggcctgcc caacgacatc attatcgagc tggcccgcga gaagaactcc     1500

aaggacgccc agaaaatgat caacgagatg cagaagcgga accggcagac caacgagcgg     1560

atcgaggaaa tcatccggac caccggcaaa gagaacgcca agtacctgat cgagaagatc     1620

aagctgcacg acatgcagga aggcaagtgc ctgtacagcc tggaagccat ccctctggaa     1680

gatctgctga acaacccctt caactatgag gtggaccaca tcatccccag aagcgtgtcc     1740

ttcgacaaca gcttcaacaa caaggtgctc gtgaagcagg aagaaaacag caagaagggc     1800

aaccggaccc cattccagta cctgagcagc agcgacagca agatcagcta cgaaaccttc     1860

aagaagcaca tcctgaatct ggccaagggc aagggcagaa tcagcaagac caagaaagag     1920

tatctgctgg aagaacggga catcaacagg ttctccgtgc agaaagactt catcaaccgg     1980

aacctggtgg ataccagata cgccaccaga ggcctgatga acctgctgcg gagctacttc     2040

agagtgaaca acctggacgt gaaagtgaag tccatcaatg gcggcttcac cagctttctg     2100

cggcggaagt ggaagtttaa gaaagagcgg aacaaggggt acaagcacca cgccgaggac     2160

gccctgatca ttgccaacgc cgatttcatc ttcaaagagt ggaagaaact ggacaaggcc     2220

aaaaaagtga tggaaaacca gatgttcgag gaaaagcagg ccgagagcat gcccgagatc     2280

gaaaccgagc aggagtacaa agagatcttc atcacccccc accagatcaa gcacattaag     2340

gacttcaagg actacaagta cagccaccgg gtggacaaga agcctaatag agagctgatt     2400

aacgacaccc tgtactccac ccggaaggac gacaagggca acaccctgat cgtgaacaat     2460

ctgaacggcc tgtacgacaa ggacaatgac aagctgaaaa agctgatcaa caagagcccc     2520

gaaaagctgc tgatgtacca ccacgacccc cagacctacc agaaactgaa gctgattatg     2580

gaacagtacg gcgacgagaa gaatcccctg tacaagtact acgaggaaac cgggaactac     2640

ctgaccaagt actccaaaaa ggacaacggc cccgtgatca agaagattaa gtattacggc     2700

aacaaactga acgcccatct ggacatcacc gacgactacc ccaacagcag aaacaaggtc     2760

gtgaagctgt ccctgaagcc ctacagattc gacgtgtacc tggacaatgg cgtgtacaag     2820

ttcgtgaccg tgaagaatct ggatgtgatc aaaaaagaaa actactacga agtgaatagc     2880

aagtgctatg aggaagctaa gaagctgaag aagatcagca accaggccga gtttatcgcc     2940

tccttctaca acaacgatct gatcaagatc aacggcgagc tgtatagagt gatcggcgtg     3000

aacaacgacc tgctgaaccg gatcgaagtg aacatgatcg acatcaccta ccgcgagtac     3060

ctggaaaaca tgaacgacaa gaggcccccc aggatcatta agacaatcgc ctccaagacc     3120

cagagcatta agaagtacag cacagacatt ctgggcaacc tgtatgaagt gaaatctaag     3180

aagcaccctc agatcatcaa aaagggc                                         3207


<210> 61
<211> 3708
<212> DNA
<213> Artificial Sequence


<220> 
<223> cpf1

<400> 61
atggccccaa agaagaagcg gaaggtcagc aagctggaga agtttacaaa ctgctactcc       60

ctgtctaaga ccctgaggtt caaggccatc cctgtgggca agacccagga gaacatcgac      120

aataagcggc tgctggtgga ggacgagaag agagccgagg attataaggg cgtgaagaag      180

ctgctggatc gctactatct gtcttttatc aacgacgtgc tgcacagcat caagctgaag      240

aatctgaaca attacatcag cctgttccgg aagaaaacca gaaccgagaa ggagaataag      300

gagctggaga acctggagat caatctgcgg aaggagatcg ccaaggcctt caagggcaac      360

gagggctaca agtccctgtt taagaaggat atcatcgaga caatcctgcc agagttcctg      420

gacgataagg acgagatcgc cctggtgaac agcttcaatg gctttaccac agccttcacc      480

ggcttctttg ataacagaga gaatatgttt tccgaggagg ccaagagcac atccatcgcc      540

ttcaggtgta tcaacgagaa tctgacccgc tacatctcta atatggacat cttcgagaag      600

gtggacgcca tctttgataa gcacgaggtg caggagatca aggagaagat cctgaacagc      660

gactatgatg tggaggattt ctttgagggc gagttcttta actttgtgct gacacaggag      720

ggcatcgacg tgtataacgc catcatcggc ggcttcgtga ccgagagcgg cgagaagatc      780

aagggcctga acgagtacat caacctgtat aatcagaaaa ccaagcagaa gctgcctaag      840

tttaagccac tgtataagca ggtgctgagc gatcgggagt ctctgagctt ctacggcgag      900

ggctatacat ccgatgagga ggtgctggag gtgtttagaa acaccctgaa caagaacagc      960

gagatcttca gctccatcaa gaagctggag aagctgttca agaattttga cgagtactct     1020

agcgccggca tctttgtgaa gaacggcccc gccatcagca caatctccaa ggatatcttc     1080

ggcgagtgga acgtgatccg ggacaagtgg aatgccgagt atgacgatat ccacctgaag     1140

aagaaggccg tggtgaccga gaagtacgag gacgatcgga gaaagtcctt caagaagatc     1200

ggctcctttt ctctggagca gctgcaggag tacgccgacg ccgatctgtc tgtggtggag     1260

aagctgaagg agatcatcat ccagaaggtg gatgagatct acaaggtgta tggctcctct     1320

gagaagctgt tcgacgccga ttttgtgctg gagaagagcc tgaagaagaa cgacgccgtg     1380

gtggccatca tgaaggacct gctggattct gtgaagagct tcgagaatta catcaaggcc     1440

ttctttggcg agggcaagga gacaaacagg gacgagtcct tctatggcga ttttgtgctg     1500

gcctacgaca tcctgctgaa ggtggaccac atctacgatg ccatccgcaa ttatgtgacc     1560

cagaagccct actctaagga taagttcaag ctgtattttc agaaccctca gttcatgggc     1620

ggctgggaca aggataagga gacagactat cgggccacca tcctgagata cggctccaag     1680

tactatctgg ccatcatgga taagaagtac gccaagtgcc tgcagaagat cgacaaggac     1740

gatgtgaacg gcaattacga gaagatcaac tataagctgc tgcccggccc taataagatg     1800

ctgccaaagg tgttcttttc taagaagtgg atggcctact ataaccccag cgaggacatc     1860

cagaagatct acaagaatgg cacattcaag aagggcgata tgtttaacct gaatgactgt     1920

cacaagctga tcgacttctt taaggatagc atctcccggt atccaaagtg gtccaatgcc     1980

tacgatttca acttttctga gacagagaag tataaggaca tcgccggctt ttacagagag     2040

gtggaggagc agggctataa ggtgagcttc gagtctgcca gcaagaagga ggtggataag     2100

ctggtggagg agggcaagct gtatatgttc cagatctata acaaggactt ttccgataag     2160

tctcacggca cacccaatct gcacaccatg tacttcaagc tgctgtttga cgagaacaat     2220

cacggacaga tcaggctgag cggaggagca gagctgttca tgaggcgcgc ctccctgaag     2280

aaggaggagc tggtggtgca cccagccaac tcccctatcg ccaacaagaa tccagataat     2340

cccaagaaaa ccacaaccct gtcctacgac gtgtataagg ataagaggtt ttctgaggac     2400

cagtacgagc tgcacatccc aatcgccatc aataagtgcc ccaagaacat cttcaagatc     2460

aatacagagg tgcgcgtgct gctgaagcac gacgataacc cctatgtgat cggcatcgat     2520

aggggcgagc gcaatctgct gtatatcgtg gtggtggacg gcaagggcaa catcgtggag     2580

cagtattccc tgaacgagat catcaacaac ttcaacggca tcaggatcaa gacagattac     2640

cactctctgc tggacaagaa ggagaaggag aggttcgagg cccgccagaa ctggacctcc     2700

atcgagaata tcaaggagct gaaggccggc tatatctctc aggtggtgca caagatctgc     2760

gagctggtgg agaagtacga tgccgtgatc gccctggagg acctgaactc tggctttaag     2820

aatagccgcg tgaaggtgga gaagcaggtg tatcagaagt tcgagaagat gctgatcgat     2880

aagctgaact acatggtgga caagaagtct aatccttgtg caacaggcgg cgccctgaag     2940

ggctatcaga tcaccaataa gttcgagagc tttaagtcca tgtctaccca gaacggcttc     3000

atcttttaca tccctgcctg gctgacatcc aagatcgatc catctaccgg ctttgtgaac     3060

ctgctgaaaa ccaagtatac cagcatcgcc gattccaaga agttcatcag ctcctttgac     3120

aggatcatgt acgtgcccga ggaggatctg ttcgagtttg ccctggacta taagaacttc     3180

tctcgcacag acgccgatta catcaagaag tggaagctgt actcctacgg caaccggatc     3240

agaatcttcc ggaatcctaa gaagaacaac gtgttcgact gggaggaggt gtgcctgacc     3300

agcgcctata aggagctgtt caacaagtac ggcatcaatt atcagcaggg cgatatcaga     3360

gccctgctgt gcgagcagtc cgacaaggcc ttctactcta gctttatggc cctgatgagc     3420

ctgatgctgc agatgcggaa cagcatcaca ggccgcaccg acgtggattt tctgatcagc     3480

cctgtgaaga actccgacgg catcttctac gatagccgga actatgaggc ccaggagaat     3540

gccatcctgc caaagaacgc cgacgccaat ggcgcctata acatcgccag aaaggtgctg     3600

tgggccatcg gccagttcaa gaaggccgag gacgagaagc tggataaggt gaagatcgcc     3660

atctctaaca aggagtggct ggagtacgcc cagaccagcg tgaagcac                  3708


<210> 62
<211> 2970
<212> DNA
<213> Artificial Sequence


<220> 
<223> CasX

<400> 62
atggccccaa agaagaagcg gaaggtcagc atgcaagaga tcaagagaat caacaagatc      60

agaaggagac tggtcaagga cagcaacaca aagaaggccg gcaagacagg ccccatgaaa     120

accctgctcg tcagagtgat gacccctgac ctgagagagc ggctggaaaa cctgagaaag     180

aagcccgaga acatccctca gcctatcagc aacaccagca gggccaacct gaacaagctg     240

ctgaccgact acaccgagat gaagaaagcc atcctgcacg tgtactggga agagttccag     300

aaagaccccg tgggcctgat gagcagagtt gctcagcccg ctcctaagaa catcgaccag     360

agaaagctga tccccgtgaa ggacggcaac gagagactga cctctagcgg ctttgcctgc     420

agccagtgtt gccagcctct gtacgtgtac aagctggaac aagtgaacga caagggcaag     480

ccccacacca actacttcgg cagatgcaac gtgtccgagc acgagaggct gatcctgctg     540

tctcctcaca agcccgaggc caacgatgag ctggtcacat acagcctggg caagttcgga     600

cagagagccc tggacttcta cagcatccac gtgaccaggg agagcaatca ccctgtgaag     660

cccctggaac agatcggcgg caatagctgt gcctctggac ctgtgggaaa agccctgagc     720

gacgcctgta tgggagccgt ggcatccttc ctgaccaagt accaggacat catcctggaa     780

caccagaaag tgatcaagaa gaacgagaaa agactggcca acctcaagga tatcgccagc     840

gctaacggcc tggcctttcc taagatcacc ctgcctccac agcctcacac caaagagggc     900

atcgaggcct acaacaacgt ggtggcccag atcgtgattt gggtcaacct gaatctgtgg     960

cagaagctga agatcggcag ggacgaagcc aagccactgc agagactgaa gggcttccct    1020

agcttccctc tggtggaaag acaggccaat gaagtggatt ggtgggacat ggtctgcaac    1080

gtgaagaagc tgatcaacga gaagaaagag gatggcaagg ttttctggca gaacctggcc    1140

ggctacaaga gacaagaagc cctgctgcct tacctgagca gcgaagagga ccggaagaag    1200

ggcaagaagt tcgccagata ccagttcggc gacctgctgc tgcacctgga aaagaagcac    1260

ggcgaggact ggggcaaagt gtacgatgag gcctgggaga gaatcgacaa gaaggtggaa    1320

ggcctgagca agcacattaa gctggaagag gaaagaagga gcgaggacgc ccaatctaaa    1380

gccgctctga ccgattggct gagagccaag gccagctttg tgatcgaggg cctgaaagag    1440

gccgacaagg acgagttctg cagatgcgag ctgaagctgc agaagtggta cggcgatctg    1500

agaggcaagc ccttcgccat tgaggccgag aacagcatcc tggacatcag cggcttcagc    1560

aagcagtaca actgcgcctt catttggcag aaagacggcg tcaagaaact gaacctgtac    1620

ctgatcatca attacttcaa aggcggcaag ctgcggttca agaagatcaa acccgaggcc    1680

ttcgaggcta acagattcta caccgtgatc aacaaaaagt ccggcgagat cgtgcccatg    1740

gaagtgaact tcaacttcga cgaccccaac ctgattatcc tgcctctggc cttcggcaag    1800

agacagggca gagagttcat ctggaacgat ctgctgagcc tggaaaccgg ctctctgaag    1860

ctggccaatg gcagagtgat cgagaaaacc ctgtacaaca ggagaaccag acaggacgag    1920

cctgctctgt ttgtggccct gaccttcgag agaagagagg tgctggacag cagcaacatc    1980

aagcccatga acctgatcgg catcgaccgg ggcgagaata tccctgctgt gatcgccctg    2040

acagaccctg aaggatgccc actgagcaga ttcaaggact ccctgggcaa ccctacacac    2100

atcctgagaa tcggcgagag ctacaaagag aagcagagga caatccaggc cgccaaagag    2160

gtggaacaga gaagagccgg cggatactct aggaagtacg ccagcaaggc caagaatctg    2220

gccgacgaca tggtccgaaa caccgccaga gatctgctgt actacgccgt gacacaggac    2280

gccatgctga tcttcgagaa tctgagcaga ggcttcggcc ggcagggcaa gagaaccttt    2340

atggccgaga ggcagtacac cagaatggaa gattggctca cagctaaact ggcctacgag    2400

ggactgccca gcaagaccta cctgtccaaa acactggccc agtatacctc caagacctgc    2460

agcaattgcg gcttcaccat caccagcgcc gactacgaca gagtgctgga aaagctcaag    2520

aaaaccgcca ccggctggat gaccaccatc aacggcaaag agctgaaggt tgagggccag    2580

atcacctact acaacaggta caagaggcag aacgtcgtga aggatctgag cgtggaactg    2640

gacagactga gcgaagagag cgtgaacaac gacatcagca gctggacaaa gggcagatca    2700

ggcgaggctc tgagcctgct gaagaagagg tttagccaca gacctgtgca agagaagttc    2760

gtgtgcctga actgcggctt cgagacacac gccgatgaac aggctgccct gaacattgcc    2820

agaagctggc tgttcctgag aagccaagag tacaagaagt accagaccaa caagaccacc    2880

ggcaacaccg acaagagggc ctttgtggaa acctggcaga gcttctacag aaaaaagctg    2940

aaagaagtct ggaagcccgc cgtgactagt                                     2970


<210> 63
<211> 2976
<212> DNA
<213> Campylobacter jejuni


<400> 63
atggccccaa agaagaagcg gaaggtcgcc agaatcctgg ccttcgacat cggcatcagc      60

agcatcggct gggccttcag cgagaacgac gagctgaagg actgcggcgt gcggatcttc     120

accaaggtgg aaaaccccaa gaccggcgag agcctggccc tgcccagaag gctggccaga     180

agcgcccgga agagactggc cagacggaag gcccggctga accacctgaa gcacctgatc     240

gccaacgagt tcaagctgaa ctacgaggac taccagagct tcgacgagtc cctggccaag     300

gcctacaagg gcagcctgat cagcccctac gagctgcggt tccgggccct gaacgagctg     360

ctgagcaagc aggacttcgc cagagtgatc ctgcacattg ccaagcggag aggctacgac     420

gacatcaaga acagcgacga caaagagaag ggcgccatcc tgaaggccat caagcagaac     480

gaggaaaagc tggccaacta ccagtccgtg ggcgagtacc tgtacaaaga gtacttccag     540

aagttcaaag agaacagcaa agaattcacc aacgtgcgga acaagaaaga aagctacgag     600

cggtgtatcg cccagagctt cctgaaggat gagctgaagc tgatcttcaa gaagcagaga     660

gagttcggct tcagcttcag caagaaattc gaggaagagg tgctgagcgt cgccttctac     720

aagagagccc tgaaggactt cagccacctc gtgggcaact gcagcttctt caccgacgag     780

aagagagccc ccaagaacag ccccctggcc ttcatgttcg tggccctgac ccggatcatc     840

aacctgctga acaatctgaa gaacaccgag ggcatcctgt acaccaagga cgacctgaac     900

gccctgctga atgaggtgct gaagaacggc accctgacct acaagcagac caagaagctg     960

ctgggcctga gcgacgacta cgagtttaag ggcgagaagg gcacctactt catcgagttc    1020

aagaagtaca aagagttcat caaggccctg ggcgagcaca acctgagcca ggacgatctg    1080

aatgagatcg ccaaggacat caccctgatc aaggacgaga ttaagctgaa gaaggccctg    1140

gccaaatacg acctgaatca gaaccagatc gacagcctga gcaagctgga attcaaggat    1200

cacctgaaca tcagcttcaa ggctctgaag ctggtcaccc ccctgatgct ggaaggcaag    1260

aagtacgacg aggcctgcaa cgagctgaac ctgaaggtgg ccatcaacga ggacaagaag    1320

gacttcctgc ccgccttcaa cgaaacctac tacaaggacg aagtgaccaa ccccgtggtg    1380

ctgcgggcca tcaaagaata ccggaaggtg ctgaatgccc tgctcaagaa atacggcaag    1440

gtgcacaaga tcaacatcga gctggcccgg gaagtgggca agaaccacag ccagcgggcc    1500

aagatcgaga aagagcagaa cgaaaactac aaggccaaga aggacgctga gctggaatgc    1560

gagaagctgg gactgaagat caacagcaag aacatcctga agctgcggct gttcaaagaa    1620

cagaaagagt tctgcgccta cagcggcgag aagatcaaga tcagcgatct gcaggacgag    1680

aagatgctgg aaatcgacca catctacccc tacagccggt ccttcgacga cagctacatg    1740

aacaaggtgc tggtgttcac caaacagaac caggaaaaac tgaaccagac ccccttcgag    1800

gccttcggca acgacagcgc caagtggcag aaaatcgagg tgctggccaa gaacctgccc    1860

accaagaaac agaagagaat cctggacaag aattacaagg acaaagagca gaagaacttc    1920

aaggaccgga acctgaacga cacccggtat atcgcccggc tggtgctgaa ctacacaaag    1980

gactacctgg atttcctgcc cctgtccgac gacgagaaca ccaagctgaa cgatacccag    2040

aaaggctcca aggtgcacgt ggaagccaag agcggcatgc tgaccagcgc cctgagacac    2100

acctggggct tcagcgccaa ggatcggaac aaccatctgc accacgccat cgacgccgtg    2160

atcattgcct acgccaacaa cagcatcgtg aaggccttct ccgacttcaa gaaagaacag    2220

gaaagcaaca gcgccgagct gtacgccaag aagatctctg agctggacta caagaacaag    2280

cggaagttct tcgagccctt cagcggcttc cggcagaagg tgctggataa gatcgacgag    2340

atcttcgtgt ccaagcccga gcggaagaag ccctctggcg ccctgcacga ggaaaccttc    2400

agaaaagagg aagagttcta ccagtcctac ggcggcaaag aaggcgtgct gaaggccctc    2460

gagctgggca agatcagaaa agtgaacggc aagatcgtga agaacgggga catgttccgg    2520

gtggacatct tcaagcacaa aaagaccaac aagttctacg ccgtgcccat ctacaccatg    2580

gacttcgccc tgaaggtgct gcccaacaag gccgtggccc ggtccaagaa gggcgagatc    2640

aaggactgga ttctgatgga cgagaactac gagttctgct ttagcctgta caaggactcc    2700

ctgatcctga tccagaccaa ggacatgcag gaacccgagt tcgtctacta caacgccttc    2760

accagcagca ccgtgtccct gatcgtgtct aagcacgaca acaagttcga gacactgagc    2820

aagaaccaga agatcctgtt caagaacgcc aacgagaaag aagtgatcgc caagagcatc    2880

ggcatccaga atctgaaggt gttcgagaag tacatcgtgt ccgccctggg agaagtgaca    2940

aaggccgagt tccggcagag agaggacttc aaaaag                              2976


<210> 64
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> human Cas9 (hCas9) amino acid

<400> 64
Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         
His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             
His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 
Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     
Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         
Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     
Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     
Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         
Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             
Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 
Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 
Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 
Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     
Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             
Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 
His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 
Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 
His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 
Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         
Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             
Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 
Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 
Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu Glu Ser Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys Met Ile Ala Lys 
    1010                1015                1020                
Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu Ala Asn Gly Glu 
                1045                1050                1055    
Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu Lys Gly 
            1140                1145                1150        
Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp Phe Leu Glu Ala 
    1170                1175                1180                
Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg Met Leu Ala Ser 
                1205                1210                1215    
Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu Pro Ser Lys Tyr 
            1220                1225                1230        
Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys Leu Lys Gly Ser 
        1235                1240                1245            
Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu Gln His Lys His 
    1250                1255                1260                
Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe Ser Lys Arg Val 
1265                1270                1275                1280
Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser Ala Tyr Asn Lys 
                1285                1290                1295    
His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn Ile Ile His Leu 
            1300                1305                1310        
Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe Lys Tyr Phe Asp 
        1315                1320                1325            
Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu Asp 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Gln Leu Gly Gly Asp 
                1365            

<210> 65
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> nickase Cas9 (nCas9) amino acid

<400> 65
Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         
His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             
His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 
Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     
Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         
Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     
Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     
Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         
Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             
Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 
Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 
Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 
Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     
Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             
Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 
His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 
Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 
His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 
Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         
Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             
Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 
Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 
Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu Glu Ser Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys Met Ile Ala Lys 
    1010                1015                1020                
Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu Ala Asn Gly Glu 
                1045                1050                1055    
Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu Lys Gly 
            1140                1145                1150        
Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp Phe Leu Glu Ala 
    1170                1175                1180                
Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg Met Leu Ala Ser 
                1205                1210                1215    
Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu Pro Ser Lys Tyr 
            1220                1225                1230        
Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys Leu Lys Gly Ser 
        1235                1240                1245            
Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu Gln His Lys His 
    1250                1255                1260                
Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe Ser Lys Arg Val 
1265                1270                1275                1280
Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser Ala Tyr Asn Lys 
                1285                1290                1295    
His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn Ile Ile His Leu 
            1300                1305                1310        
Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe Lys Tyr Phe Asp 
        1315                1320                1325            
Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu Asp 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Gln Leu Gly Gly Asp 
                1365            

<210> 66
<211> 1069
<212> PRT
<213> Staphylococcus aureus


<400> 66
Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      
Ala Lys Arg Asn Tyr Ile Leu Gly Leu Ala Ile Gly Ile Thr Ser Val 
            20                  25                  30          
Gly Tyr Gly Ile Ile Asp Tyr Glu Thr Arg Asp Val Ile Asp Ala Gly 
        35                  40                  45              
Val Arg Leu Phe Lys Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg 
    50                  55                  60                  
Ser Lys Arg Gly Ala Arg Arg Leu Lys Arg Arg Arg Arg His Arg Ile 
65                  70                  75                  80  
Gln Arg Val Lys Lys Leu Leu Phe Asp Tyr Asn Leu Leu Thr Asp His 
                85                  90                  95      
Ser Glu Leu Ser Gly Ile Asn Pro Tyr Glu Ala Arg Val Lys Gly Leu 
            100                 105                 110         
Ser Gln Lys Leu Ser Glu Glu Glu Phe Ser Ala Ala Leu Leu His Leu 
        115                 120                 125             
Ala Lys Arg Arg Gly Val His Asn Val Asn Glu Val Glu Glu Asp Thr 
    130                 135                 140                 
Gly Asn Glu Leu Ser Thr Lys Glu Gln Ile Ser Arg Asn Ser Lys Ala 
145                 150                 155                 160 
Leu Glu Glu Lys Tyr Val Ala Glu Leu Gln Leu Glu Arg Leu Lys Lys 
                165                 170                 175     
Asp Gly Glu Val Arg Gly Ser Ile Asn Arg Phe Lys Thr Ser Asp Tyr 
            180                 185                 190         
Val Lys Glu Ala Lys Gln Leu Leu Lys Val Gln Lys Ala Tyr His Gln 
        195                 200                 205             
Leu Asp Gln Ser Phe Ile Asp Thr Tyr Ile Asp Leu Leu Glu Thr Arg 
    210                 215                 220                 
Arg Thr Tyr Tyr Glu Gly Pro Gly Glu Gly Ser Pro Phe Gly Trp Lys 
225                 230                 235                 240 
Asp Ile Lys Glu Trp Tyr Glu Met Leu Met Gly His Cys Thr Tyr Phe 
                245                 250                 255     
Pro Glu Glu Leu Arg Ser Val Lys Tyr Ala Tyr Asn Ala Asp Leu Tyr 
            260                 265                 270         
Asn Ala Leu Asn Asp Leu Asn Asn Leu Val Ile Thr Arg Asp Glu Asn 
        275                 280                 285             
Glu Lys Leu Glu Tyr Tyr Glu Lys Phe Gln Ile Ile Glu Asn Val Phe 
    290                 295                 300                 
Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile Ala Lys Glu Ile Leu 
305                 310                 315                 320 
Val Asn Glu Glu Asp Ile Lys Gly Tyr Arg Val Thr Ser Thr Gly Lys 
                325                 330                 335     
Pro Glu Phe Thr Asn Leu Lys Val Tyr His Asp Ile Lys Asp Ile Thr 
            340                 345                 350         
Ala Arg Lys Glu Ile Ile Glu Asn Ala Glu Leu Leu Asp Gln Ile Ala 
        355                 360                 365             
Lys Ile Leu Thr Ile Tyr Gln Ser Ser Glu Asp Ile Gln Glu Glu Leu 
    370                 375                 380                 
Thr Asn Leu Asn Ser Glu Leu Thr Gln Glu Glu Ile Glu Gln Ile Ser 
385                 390                 395                 400 
Asn Leu Lys Gly Tyr Thr Gly Thr His Asn Leu Ser Leu Lys Ala Ile 
                405                 410                 415     
Asn Leu Ile Leu Asp Glu Leu Trp His Thr Asn Asp Asn Gln Ile Ala 
            420                 425                 430         
Ile Phe Asn Arg Leu Lys Leu Val Pro Lys Lys Val Asp Leu Ser Gln 
        435                 440                 445             
Gln Lys Glu Ile Pro Thr Thr Leu Val Asp Asp Phe Ile Leu Ser Pro 
    450                 455                 460                 
Val Val Lys Arg Ser Phe Ile Gln Ser Ile Lys Val Ile Asn Ala Ile 
465                 470                 475                 480 
Ile Lys Lys Tyr Gly Leu Pro Asn Asp Ile Ile Ile Glu Leu Ala Arg 
                485                 490                 495     
Glu Lys Asn Ser Lys Asp Ala Gln Lys Met Ile Asn Glu Met Gln Lys 
            500                 505                 510         
Arg Asn Arg Gln Thr Asn Glu Arg Ile Glu Glu Ile Ile Arg Thr Thr 
        515                 520                 525             
Gly Lys Glu Asn Ala Lys Tyr Leu Ile Glu Lys Ile Lys Leu His Asp 
    530                 535                 540                 
Met Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu Ala Ile Pro Leu Glu 
545                 550                 555                 560 
Asp Leu Leu Asn Asn Pro Phe Asn Tyr Glu Val Asp His Ile Ile Pro 
                565                 570                 575     
Arg Ser Val Ser Phe Asp Asn Ser Phe Asn Asn Lys Val Leu Val Lys 
            580                 585                 590         
Gln Glu Glu Asn Ser Lys Lys Gly Asn Arg Thr Pro Phe Gln Tyr Leu 
        595                 600                 605             
Ser Ser Ser Asp Ser Lys Ile Ser Tyr Glu Thr Phe Lys Lys His Ile 
    610                 615                 620                 
Leu Asn Leu Ala Lys Gly Lys Gly Arg Ile Ser Lys Thr Lys Lys Glu 
625                 630                 635                 640 
Tyr Leu Leu Glu Glu Arg Asp Ile Asn Arg Phe Ser Val Gln Lys Asp 
                645                 650                 655     
Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg Gly Leu 
            660                 665                 670         
Met Asn Leu Leu Arg Ser Tyr Phe Arg Val Asn Asn Leu Asp Val Lys 
        675                 680                 685             
Val Lys Ser Ile Asn Gly Gly Phe Thr Ser Phe Leu Arg Arg Lys Trp 
    690                 695                 700                 
Lys Phe Lys Lys Glu Arg Asn Lys Gly Tyr Lys His His Ala Glu Asp 
705                 710                 715                 720 
Ala Leu Ile Ile Ala Asn Ala Asp Phe Ile Phe Lys Glu Trp Lys Lys 
                725                 730                 735     
Leu Asp Lys Ala Lys Lys Val Met Glu Asn Gln Met Phe Glu Glu Lys 
            740                 745                 750         
Gln Ala Glu Ser Met Pro Glu Ile Glu Thr Glu Gln Glu Tyr Lys Glu 
        755                 760                 765             
Ile Phe Ile Thr Pro His Gln Ile Lys His Ile Lys Asp Phe Lys Asp 
    770                 775                 780                 
Tyr Lys Tyr Ser His Arg Val Asp Lys Lys Pro Asn Arg Glu Leu Ile 
785                 790                 795                 800 
Asn Asp Thr Leu Tyr Ser Thr Arg Lys Asp Asp Lys Gly Asn Thr Leu 
                805                 810                 815     
Ile Val Asn Asn Leu Asn Gly Leu Tyr Asp Lys Asp Asn Asp Lys Leu 
            820                 825                 830         
Lys Lys Leu Ile Asn Lys Ser Pro Glu Lys Leu Leu Met Tyr His His 
        835                 840                 845             
Asp Pro Gln Thr Tyr Gln Lys Leu Lys Leu Ile Met Glu Gln Tyr Gly 
    850                 855                 860                 
Asp Glu Lys Asn Pro Leu Tyr Lys Tyr Tyr Glu Glu Thr Gly Asn Tyr 
865                 870                 875                 880 
Leu Thr Lys Tyr Ser Lys Lys Asp Asn Gly Pro Val Ile Lys Lys Ile 
                885                 890                 895     
Lys Tyr Tyr Gly Asn Lys Leu Asn Ala His Leu Asp Ile Thr Asp Asp 
            900                 905                 910         
Tyr Pro Asn Ser Arg Asn Lys Val Val Lys Leu Ser Leu Lys Pro Tyr 
        915                 920                 925             
Arg Phe Asp Val Tyr Leu Asp Asn Gly Val Tyr Lys Phe Val Thr Val 
    930                 935                 940                 
Lys Asn Leu Asp Val Ile Lys Lys Glu Asn Tyr Tyr Glu Val Asn Ser 
945                 950                 955                 960 
Lys Cys Tyr Glu Glu Ala Lys Lys Leu Lys Lys Ile Ser Asn Gln Ala 
                965                 970                 975     
Glu Phe Ile Ala Ser Phe Tyr Asn Asn Asp Leu Ile Lys Ile Asn Gly 
            980                 985                 990         
Glu Leu Tyr Arg Val Ile Gly Val Asn Asn Asp Leu Leu Asn Arg Ile 
        995                 1000                1005            
Glu Val Asn Met Ile Asp Ile Thr Tyr Arg Glu Tyr Leu Glu Asn Met 
    1010                1015                1020                
Asn Asp Lys Arg Pro Pro Arg Ile Ile Lys Thr Ile Ala Ser Lys Thr 
1025                1030                1035                1040
Gln Ser Ile Lys Lys Tyr Ser Thr Asp Ile Leu Gly Asn Leu Tyr Glu 
                1045                1050                1055    
Val Lys Ser Lys Lys His Pro Gln Ile Ile Lys Lys Gly 
            1060                1065                

<210> 67
<211> 1368
<212> PRT
<213> Artificial Sequence


<220> 
<223> dead Cas9 (dCas9) amino acid

<400> 67
Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         
His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             
His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 
Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     
Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         
Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     
Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     
Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         
Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             
Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 
Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 
Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 
Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     
Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             
Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 
His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 
Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 
His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 
Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         
Leu Ser Asp Tyr Asp Val Ala Ala Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             
Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Ala Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 
Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 
Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu Glu Ser Glu Phe 
        995                 1000                1005            
Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys Met Ile Ala Lys 
    1010                1015                1020                
Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser 
1025                1030                1035                1040
Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu Ala Asn Gly Glu 
                1045                1050                1055    
Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu Thr Gly Glu Ile 
            1060                1065                1070        
Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg Lys Val Leu Ser 
        1075                1080                1085            
Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly 
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser Asp Lys Leu Ile 
1105                1110                1115                1120
Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser 
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu Lys Gly 
            1140                1145                1150        
Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu Gly Ile Thr Ile 
        1155                1160                1165            
Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp Phe Leu Glu Ala 
    1170                1175                1180                
Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile Lys Leu Pro Lys 
1185                1190                1195                1200
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg Met Leu Ala Ser 
                1205                1210                1215    
Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu Pro Ser Lys Tyr 
            1220                1225                1230        
Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys Leu Lys Gly Ser 
        1235                1240                1245            
Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu Gln His Lys His 
    1250                1255                1260                
Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe Ser Lys Arg Val 
1265                1270                1275                1280
Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser Ala Tyr Asn Lys 
                1285                1290                1295    
His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn Ile Ile His Leu 
            1300                1305                1310        
Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe Lys Tyr Phe Asp 
        1315                1320                1325            
Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu Asp 
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile 
1345                1350                1355                1360
Asp Leu Ser Gln Leu Gly Gly Asp 
                1365            

<210> 68
<211> 1069
<212> PRT
<213> Staphylococcus aureus


<400> 68
Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      
Ala Lys Arg Asn Tyr Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val 
            20                  25                  30          
Gly Tyr Gly Ile Ile Asp Tyr Glu Thr Arg Asp Val Ile Asp Ala Gly 
        35                  40                  45              
Val Arg Leu Phe Lys Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg 
    50                  55                  60                  
Ser Lys Arg Gly Ala Arg Arg Leu Lys Arg Arg Arg Arg His Arg Ile 
65                  70                  75                  80  
Gln Arg Val Lys Lys Leu Leu Phe Asp Tyr Asn Leu Leu Thr Asp His 
                85                  90                  95      
Ser Glu Leu Ser Gly Ile Asn Pro Tyr Glu Ala Arg Val Lys Gly Leu 
            100                 105                 110         
Ser Gln Lys Leu Ser Glu Glu Glu Phe Ser Ala Ala Leu Leu His Leu 
        115                 120                 125             
Ala Lys Arg Arg Gly Val His Asn Val Asn Glu Val Glu Glu Asp Thr 
    130                 135                 140                 
Gly Asn Glu Leu Ser Thr Lys Glu Gln Ile Ser Arg Asn Ser Lys Ala 
145                 150                 155                 160 
Leu Glu Glu Lys Tyr Val Ala Glu Leu Gln Leu Glu Arg Leu Lys Lys 
                165                 170                 175     
Asp Gly Glu Val Arg Gly Ser Ile Asn Arg Phe Lys Thr Ser Asp Tyr 
            180                 185                 190         
Val Lys Glu Ala Lys Gln Leu Leu Lys Val Gln Lys Ala Tyr His Gln 
        195                 200                 205             
Leu Asp Gln Ser Phe Ile Asp Thr Tyr Ile Asp Leu Leu Glu Thr Arg 
    210                 215                 220                 
Arg Thr Tyr Tyr Glu Gly Pro Gly Glu Gly Ser Pro Phe Gly Trp Lys 
225                 230                 235                 240 
Asp Ile Lys Glu Trp Tyr Glu Met Leu Met Gly His Cys Thr Tyr Phe 
                245                 250                 255     
Pro Glu Glu Leu Arg Ser Val Lys Tyr Ala Tyr Asn Ala Asp Leu Tyr 
            260                 265                 270         
Asn Ala Leu Asn Asp Leu Asn Asn Leu Val Ile Thr Arg Asp Glu Asn 
        275                 280                 285             
Glu Lys Leu Glu Tyr Tyr Glu Lys Phe Gln Ile Ile Glu Asn Val Phe 
    290                 295                 300                 
Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile Ala Lys Glu Ile Leu 
305                 310                 315                 320 
Val Asn Glu Glu Asp Ile Lys Gly Tyr Arg Val Thr Ser Thr Gly Lys 
                325                 330                 335     
Pro Glu Phe Thr Asn Leu Lys Val Tyr His Asp Ile Lys Asp Ile Thr 
            340                 345                 350         
Ala Arg Lys Glu Ile Ile Glu Asn Ala Glu Leu Leu Asp Gln Ile Ala 
        355                 360                 365             
Lys Ile Leu Thr Ile Tyr Gln Ser Ser Glu Asp Ile Gln Glu Glu Leu 
    370                 375                 380                 
Thr Asn Leu Asn Ser Glu Leu Thr Gln Glu Glu Ile Glu Gln Ile Ser 
385                 390                 395                 400 
Asn Leu Lys Gly Tyr Thr Gly Thr His Asn Leu Ser Leu Lys Ala Ile 
                405                 410                 415     
Asn Leu Ile Leu Asp Glu Leu Trp His Thr Asn Asp Asn Gln Ile Ala 
            420                 425                 430         
Ile Phe Asn Arg Leu Lys Leu Val Pro Lys Lys Val Asp Leu Ser Gln 
        435                 440                 445             
Gln Lys Glu Ile Pro Thr Thr Leu Val Asp Asp Phe Ile Leu Ser Pro 
    450                 455                 460                 
Val Val Lys Arg Ser Phe Ile Gln Ser Ile Lys Val Ile Asn Ala Ile 
465                 470                 475                 480 
Ile Lys Lys Tyr Gly Leu Pro Asn Asp Ile Ile Ile Glu Leu Ala Arg 
                485                 490                 495     
Glu Lys Asn Ser Lys Asp Ala Gln Lys Met Ile Asn Glu Met Gln Lys 
            500                 505                 510         
Arg Asn Arg Gln Thr Asn Glu Arg Ile Glu Glu Ile Ile Arg Thr Thr 
        515                 520                 525             
Gly Lys Glu Asn Ala Lys Tyr Leu Ile Glu Lys Ile Lys Leu His Asp 
    530                 535                 540                 
Met Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu Ala Ile Pro Leu Glu 
545                 550                 555                 560 
Asp Leu Leu Asn Asn Pro Phe Asn Tyr Glu Val Asp His Ile Ile Pro 
                565                 570                 575     
Arg Ser Val Ser Phe Asp Asn Ser Phe Asn Asn Lys Val Leu Val Lys 
            580                 585                 590         
Gln Glu Glu Asn Ser Lys Lys Gly Asn Arg Thr Pro Phe Gln Tyr Leu 
        595                 600                 605             
Ser Ser Ser Asp Ser Lys Ile Ser Tyr Glu Thr Phe Lys Lys His Ile 
    610                 615                 620                 
Leu Asn Leu Ala Lys Gly Lys Gly Arg Ile Ser Lys Thr Lys Lys Glu 
625                 630                 635                 640 
Tyr Leu Leu Glu Glu Arg Asp Ile Asn Arg Phe Ser Val Gln Lys Asp 
                645                 650                 655     
Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg Gly Leu 
            660                 665                 670         
Met Asn Leu Leu Arg Ser Tyr Phe Arg Val Asn Asn Leu Asp Val Lys 
        675                 680                 685             
Val Lys Ser Ile Asn Gly Gly Phe Thr Ser Phe Leu Arg Arg Lys Trp 
    690                 695                 700                 
Lys Phe Lys Lys Glu Arg Asn Lys Gly Tyr Lys His His Ala Glu Asp 
705                 710                 715                 720 
Ala Leu Ile Ile Ala Asn Ala Asp Phe Ile Phe Lys Glu Trp Lys Lys 
                725                 730                 735     
Leu Asp Lys Ala Lys Lys Val Met Glu Asn Gln Met Phe Glu Glu Lys 
            740                 745                 750         
Gln Ala Glu Ser Met Pro Glu Ile Glu Thr Glu Gln Glu Tyr Lys Glu 
        755                 760                 765             
Ile Phe Ile Thr Pro His Gln Ile Lys His Ile Lys Asp Phe Lys Asp 
    770                 775                 780                 
Tyr Lys Tyr Ser His Arg Val Asp Lys Lys Pro Asn Arg Glu Leu Ile 
785                 790                 795                 800 
Asn Asp Thr Leu Tyr Ser Thr Arg Lys Asp Asp Lys Gly Asn Thr Leu 
                805                 810                 815     
Ile Val Asn Asn Leu Asn Gly Leu Tyr Asp Lys Asp Asn Asp Lys Leu 
            820                 825                 830         
Lys Lys Leu Ile Asn Lys Ser Pro Glu Lys Leu Leu Met Tyr His His 
        835                 840                 845             
Asp Pro Gln Thr Tyr Gln Lys Leu Lys Leu Ile Met Glu Gln Tyr Gly 
    850                 855                 860                 
Asp Glu Lys Asn Pro Leu Tyr Lys Tyr Tyr Glu Glu Thr Gly Asn Tyr 
865                 870                 875                 880 
Leu Thr Lys Tyr Ser Lys Lys Asp Asn Gly Pro Val Ile Lys Lys Ile 
                885                 890                 895     
Lys Tyr Tyr Gly Asn Lys Leu Asn Ala His Leu Asp Ile Thr Asp Asp 
            900                 905                 910         
Tyr Pro Asn Ser Arg Asn Lys Val Val Lys Leu Ser Leu Lys Pro Tyr 
        915                 920                 925             
Arg Phe Asp Val Tyr Leu Asp Asn Gly Val Tyr Lys Phe Val Thr Val 
    930                 935                 940                 
Lys Asn Leu Asp Val Ile Lys Lys Glu Asn Tyr Tyr Glu Val Asn Ser 
945                 950                 955                 960 
Lys Cys Tyr Glu Glu Ala Lys Lys Leu Lys Lys Ile Ser Asn Gln Ala 
                965                 970                 975     
Glu Phe Ile Ala Ser Phe Tyr Asn Asn Asp Leu Ile Lys Ile Asn Gly 
            980                 985                 990         
Glu Leu Tyr Arg Val Ile Gly Val Asn Asn Asp Leu Leu Asn Arg Ile 
        995                 1000                1005            
Glu Val Asn Met Ile Asp Ile Thr Tyr Arg Glu Tyr Leu Glu Asn Met 
    1010                1015                1020                
Asn Asp Lys Arg Pro Pro Arg Ile Ile Lys Thr Ile Ala Ser Lys Thr 
1025                1030                1035                1040
Gln Ser Ile Lys Lys Tyr Ser Thr Asp Ile Leu Gly Asn Leu Tyr Glu 
                1045                1050                1055    
Val Lys Ser Lys Lys His Pro Gln Ile Ile Lys Lys Gly 
            1060                1065                

<210> 69
<211> 1236
<212> PRT
<213> Artificial Sequence


<220> 
<223> Cpf1

<400> 69
Met Ala Pro Lys Lys Lys Arg Lys Val Ser Lys Leu Glu Lys Phe Thr 
1               5                   10                  15      
Asn Cys Tyr Ser Leu Ser Lys Thr Leu Arg Phe Lys Ala Ile Pro Val 
            20                  25                  30          
Gly Lys Thr Gln Glu Asn Ile Asp Asn Lys Arg Leu Leu Val Glu Asp 
        35                  40                  45              
Glu Lys Arg Ala Glu Asp Tyr Lys Gly Val Lys Lys Leu Leu Asp Arg 
    50                  55                  60                  
Tyr Tyr Leu Ser Phe Ile Asn Asp Val Leu His Ser Ile Lys Leu Lys 
65                  70                  75                  80  
Asn Leu Asn Asn Tyr Ile Ser Leu Phe Arg Lys Lys Thr Arg Thr Glu 
                85                  90                  95      
Lys Glu Asn Lys Glu Leu Glu Asn Leu Glu Ile Asn Leu Arg Lys Glu 
            100                 105                 110         
Ile Ala Lys Ala Phe Lys Gly Asn Glu Gly Tyr Lys Ser Leu Phe Lys 
        115                 120                 125             
Lys Asp Ile Ile Glu Thr Ile Leu Pro Glu Phe Leu Asp Asp Lys Asp 
    130                 135                 140                 
Glu Ile Ala Leu Val Asn Ser Phe Asn Gly Phe Thr Thr Ala Phe Thr 
145                 150                 155                 160 
Gly Phe Phe Asp Asn Arg Glu Asn Met Phe Ser Glu Glu Ala Lys Ser 
                165                 170                 175     
Thr Ser Ile Ala Phe Arg Cys Ile Asn Glu Asn Leu Thr Arg Tyr Ile 
            180                 185                 190         
Ser Asn Met Asp Ile Phe Glu Lys Val Asp Ala Ile Phe Asp Lys His 
        195                 200                 205             
Glu Val Gln Glu Ile Lys Glu Lys Ile Leu Asn Ser Asp Tyr Asp Val 
    210                 215                 220                 
Glu Asp Phe Phe Glu Gly Glu Phe Phe Asn Phe Val Leu Thr Gln Glu 
225                 230                 235                 240 
Gly Ile Asp Val Tyr Asn Ala Ile Ile Gly Gly Phe Val Thr Glu Ser 
                245                 250                 255     
Gly Glu Lys Ile Lys Gly Leu Asn Glu Tyr Ile Asn Leu Tyr Asn Gln 
            260                 265                 270         
Lys Thr Lys Gln Lys Leu Pro Lys Phe Lys Pro Leu Tyr Lys Gln Val 
        275                 280                 285             
Leu Ser Asp Arg Glu Ser Leu Ser Phe Tyr Gly Glu Gly Tyr Thr Ser 
    290                 295                 300                 
Asp Glu Glu Val Leu Glu Val Phe Arg Asn Thr Leu Asn Lys Asn Ser 
305                 310                 315                 320 
Glu Ile Phe Ser Ser Ile Lys Lys Leu Glu Lys Leu Phe Lys Asn Phe 
                325                 330                 335     
Asp Glu Tyr Ser Ser Ala Gly Ile Phe Val Lys Asn Gly Pro Ala Ile 
            340                 345                 350         
Ser Thr Ile Ser Lys Asp Ile Phe Gly Glu Trp Asn Val Ile Arg Asp 
        355                 360                 365             
Lys Trp Asn Ala Glu Tyr Asp Asp Ile His Leu Lys Lys Lys Ala Val 
    370                 375                 380                 
Val Thr Glu Lys Tyr Glu Asp Asp Arg Arg Lys Ser Phe Lys Lys Ile 
385                 390                 395                 400 
Gly Ser Phe Ser Leu Glu Gln Leu Gln Glu Tyr Ala Asp Ala Asp Leu 
                405                 410                 415     
Ser Val Val Glu Lys Leu Lys Glu Ile Ile Ile Gln Lys Val Asp Glu 
            420                 425                 430         
Ile Tyr Lys Val Tyr Gly Ser Ser Glu Lys Leu Phe Asp Ala Asp Phe 
        435                 440                 445             
Val Leu Glu Lys Ser Leu Lys Lys Asn Asp Ala Val Val Ala Ile Met 
    450                 455                 460                 
Lys Asp Leu Leu Asp Ser Val Lys Ser Phe Glu Asn Tyr Ile Lys Ala 
465                 470                 475                 480 
Phe Phe Gly Glu Gly Lys Glu Thr Asn Arg Asp Glu Ser Phe Tyr Gly 
                485                 490                 495     
Asp Phe Val Leu Ala Tyr Asp Ile Leu Leu Lys Val Asp His Ile Tyr 
            500                 505                 510         
Asp Ala Ile Arg Asn Tyr Val Thr Gln Lys Pro Tyr Ser Lys Asp Lys 
        515                 520                 525             
Phe Lys Leu Tyr Phe Gln Asn Pro Gln Phe Met Gly Gly Trp Asp Lys 
    530                 535                 540                 
Asp Lys Glu Thr Asp Tyr Arg Ala Thr Ile Leu Arg Tyr Gly Ser Lys 
545                 550                 555                 560 
Tyr Tyr Leu Ala Ile Met Asp Lys Lys Tyr Ala Lys Cys Leu Gln Lys 
                565                 570                 575     
Ile Asp Lys Asp Asp Val Asn Gly Asn Tyr Glu Lys Ile Asn Tyr Lys 
            580                 585                 590         
Leu Leu Pro Gly Pro Asn Lys Met Leu Pro Lys Val Phe Phe Ser Lys 
        595                 600                 605             
Lys Trp Met Ala Tyr Tyr Asn Pro Ser Glu Asp Ile Gln Lys Ile Tyr 
    610                 615                 620                 
Lys Asn Gly Thr Phe Lys Lys Gly Asp Met Phe Asn Leu Asn Asp Cys 
625                 630                 635                 640 
His Lys Leu Ile Asp Phe Phe Lys Asp Ser Ile Ser Arg Tyr Pro Lys 
                645                 650                 655     
Trp Ser Asn Ala Tyr Asp Phe Asn Phe Ser Glu Thr Glu Lys Tyr Lys 
            660                 665                 670         
Asp Ile Ala Gly Phe Tyr Arg Glu Val Glu Glu Gln Gly Tyr Lys Val 
        675                 680                 685             
Ser Phe Glu Ser Ala Ser Lys Lys Glu Val Asp Lys Leu Val Glu Glu 
    690                 695                 700                 
Gly Lys Leu Tyr Met Phe Gln Ile Tyr Asn Lys Asp Phe Ser Asp Lys 
705                 710                 715                 720 
Ser His Gly Thr Pro Asn Leu His Thr Met Tyr Phe Lys Leu Leu Phe 
                725                 730                 735     
Asp Glu Asn Asn His Gly Gln Ile Arg Leu Ser Gly Gly Ala Glu Leu 
            740                 745                 750         
Phe Met Arg Arg Ala Ser Leu Lys Lys Glu Glu Leu Val Val His Pro 
        755                 760                 765             
Ala Asn Ser Pro Ile Ala Asn Lys Asn Pro Asp Asn Pro Lys Lys Thr 
    770                 775                 780                 
Thr Thr Leu Ser Tyr Asp Val Tyr Lys Asp Lys Arg Phe Ser Glu Asp 
785                 790                 795                 800 
Gln Tyr Glu Leu His Ile Pro Ile Ala Ile Asn Lys Cys Pro Lys Asn 
                805                 810                 815     
Ile Phe Lys Ile Asn Thr Glu Val Arg Val Leu Leu Lys His Asp Asp 
            820                 825                 830         
Asn Pro Tyr Val Ile Gly Ile Asp Arg Gly Glu Arg Asn Leu Leu Tyr 
        835                 840                 845             
Ile Val Val Val Asp Gly Lys Gly Asn Ile Val Glu Gln Tyr Ser Leu 
    850                 855                 860                 
Asn Glu Ile Ile Asn Asn Phe Asn Gly Ile Arg Ile Lys Thr Asp Tyr 
865                 870                 875                 880 
His Ser Leu Leu Asp Lys Lys Glu Lys Glu Arg Phe Glu Ala Arg Gln 
                885                 890                 895     
Asn Trp Thr Ser Ile Glu Asn Ile Lys Glu Leu Lys Ala Gly Tyr Ile 
            900                 905                 910         
Ser Gln Val Val His Lys Ile Cys Glu Leu Val Glu Lys Tyr Asp Ala 
        915                 920                 925             
Val Ile Ala Leu Glu Asp Leu Asn Ser Gly Phe Lys Asn Ser Arg Val 
    930                 935                 940                 
Lys Val Glu Lys Gln Val Tyr Gln Lys Phe Glu Lys Met Leu Ile Asp 
945                 950                 955                 960 
Lys Leu Asn Tyr Met Val Asp Lys Lys Ser Asn Pro Cys Ala Thr Gly 
                965                 970                 975     
Gly Ala Leu Lys Gly Tyr Gln Ile Thr Asn Lys Phe Glu Ser Phe Lys 
            980                 985                 990         
Ser Met Ser Thr Gln Asn Gly Phe Ile Phe Tyr Ile Pro Ala Trp Leu 
        995                 1000                1005            
Thr Ser Lys Ile Asp Pro Ser Thr Gly Phe Val Asn Leu Leu Lys Thr 
    1010                1015                1020                
Lys Tyr Thr Ser Ile Ala Asp Ser Lys Lys Phe Ile Ser Ser Phe Asp 
1025                1030                1035                1040
Arg Ile Met Tyr Val Pro Glu Glu Asp Leu Phe Glu Phe Ala Leu Asp 
                1045                1050                1055    
Tyr Lys Asn Phe Ser Arg Thr Asp Ala Asp Tyr Ile Lys Lys Trp Lys 
            1060                1065                1070        
Leu Tyr Ser Tyr Gly Asn Arg Ile Arg Ile Phe Arg Asn Pro Lys Lys 
        1075                1080                1085            
Asn Asn Val Phe Asp Trp Glu Glu Val Cys Leu Thr Ser Ala Tyr Lys 
    1090                1095                1100                
Glu Leu Phe Asn Lys Tyr Gly Ile Asn Tyr Gln Gln Gly Asp Ile Arg 
1105                1110                1115                1120
Ala Leu Leu Cys Glu Gln Ser Asp Lys Ala Phe Tyr Ser Ser Phe Met 
                1125                1130                1135    
Ala Leu Met Ser Leu Met Leu Gln Met Arg Asn Ser Ile Thr Gly Arg 
            1140                1145                1150        
Thr Asp Val Asp Phe Leu Ile Ser Pro Val Lys Asn Ser Asp Gly Ile 
        1155                1160                1165            
Phe Tyr Asp Ser Arg Asn Tyr Glu Ala Gln Glu Asn Ala Ile Leu Pro 
    1170                1175                1180                
Lys Asn Ala Asp Ala Asn Gly Ala Tyr Asn Ile Ala Arg Lys Val Leu 
1185                1190                1195                1200
Trp Ala Ile Gly Gln Phe Lys Lys Ala Glu Asp Glu Lys Leu Asp Lys 
                1205                1210                1215    
Val Lys Ile Ala Ile Ser Asn Lys Glu Trp Leu Glu Tyr Ala Gln Thr 
            1220                1225                1230        
Ser Val Lys His 
        1235    

<210> 70
<211> 990
<212> PRT
<213> Artificial Sequence


<220> 
<223> Casx

<400> 70
Met Ala Pro Lys Lys Lys Arg Lys Val Ser Met Gln Glu Ile Lys Arg 
1               5                   10                  15      
Ile Asn Lys Ile Arg Arg Arg Leu Val Lys Asp Ser Asn Thr Lys Lys 
            20                  25                  30          
Ala Gly Lys Thr Gly Pro Met Lys Thr Leu Leu Val Arg Val Met Thr 
        35                  40                  45              
Pro Asp Leu Arg Glu Arg Leu Glu Asn Leu Arg Lys Lys Pro Glu Asn 
    50                  55                  60                  
Ile Pro Gln Pro Ile Ser Asn Thr Ser Arg Ala Asn Leu Asn Lys Leu 
65                  70                  75                  80  
Leu Thr Asp Tyr Thr Glu Met Lys Lys Ala Ile Leu His Val Tyr Trp 
                85                  90                  95      
Glu Glu Phe Gln Lys Asp Pro Val Gly Leu Met Ser Arg Val Ala Gln 
            100                 105                 110         
Pro Ala Pro Lys Asn Ile Asp Gln Arg Lys Leu Ile Pro Val Lys Asp 
        115                 120                 125             
Gly Asn Glu Arg Leu Thr Ser Ser Gly Phe Ala Cys Ser Gln Cys Cys 
    130                 135                 140                 
Gln Pro Leu Tyr Val Tyr Lys Leu Glu Gln Val Asn Asp Lys Gly Lys 
145                 150                 155                 160 
Pro His Thr Asn Tyr Phe Gly Arg Cys Asn Val Ser Glu His Glu Arg 
                165                 170                 175     
Leu Ile Leu Leu Ser Pro His Lys Pro Glu Ala Asn Asp Glu Leu Val 
            180                 185                 190         
Thr Tyr Ser Leu Gly Lys Phe Gly Gln Arg Ala Leu Asp Phe Tyr Ser 
        195                 200                 205             
Ile His Val Thr Arg Glu Ser Asn His Pro Val Lys Pro Leu Glu Gln 
    210                 215                 220                 
Ile Gly Gly Asn Ser Cys Ala Ser Gly Pro Val Gly Lys Ala Leu Ser 
225                 230                 235                 240 
Asp Ala Cys Met Gly Ala Val Ala Ser Phe Leu Thr Lys Tyr Gln Asp 
                245                 250                 255     
Ile Ile Leu Glu His Gln Lys Val Ile Lys Lys Asn Glu Lys Arg Leu 
            260                 265                 270         
Ala Asn Leu Lys Asp Ile Ala Ser Ala Asn Gly Leu Ala Phe Pro Lys 
        275                 280                 285             
Ile Thr Leu Pro Pro Gln Pro His Thr Lys Glu Gly Ile Glu Ala Tyr 
    290                 295                 300                 
Asn Asn Val Val Ala Gln Ile Val Ile Trp Val Asn Leu Asn Leu Trp 
305                 310                 315                 320 
Gln Lys Leu Lys Ile Gly Arg Asp Glu Ala Lys Pro Leu Gln Arg Leu 
                325                 330                 335     
Lys Gly Phe Pro Ser Phe Pro Leu Val Glu Arg Gln Ala Asn Glu Val 
            340                 345                 350         
Asp Trp Trp Asp Met Val Cys Asn Val Lys Lys Leu Ile Asn Glu Lys 
        355                 360                 365             
Lys Glu Asp Gly Lys Val Phe Trp Gln Asn Leu Ala Gly Tyr Lys Arg 
    370                 375                 380                 
Gln Glu Ala Leu Leu Pro Tyr Leu Ser Ser Glu Glu Asp Arg Lys Lys 
385                 390                 395                 400 
Gly Lys Lys Phe Ala Arg Tyr Gln Phe Gly Asp Leu Leu Leu His Leu 
                405                 410                 415     
Glu Lys Lys His Gly Glu Asp Trp Gly Lys Val Tyr Asp Glu Ala Trp 
            420                 425                 430         
Glu Arg Ile Asp Lys Lys Val Glu Gly Leu Ser Lys His Ile Lys Leu 
        435                 440                 445             
Glu Glu Glu Arg Arg Ser Glu Asp Ala Gln Ser Lys Ala Ala Leu Thr 
    450                 455                 460                 
Asp Trp Leu Arg Ala Lys Ala Ser Phe Val Ile Glu Gly Leu Lys Glu 
465                 470                 475                 480 
Ala Asp Lys Asp Glu Phe Cys Arg Cys Glu Leu Lys Leu Gln Lys Trp 
                485                 490                 495     
Tyr Gly Asp Leu Arg Gly Lys Pro Phe Ala Ile Glu Ala Glu Asn Ser 
            500                 505                 510         
Ile Leu Asp Ile Ser Gly Phe Ser Lys Gln Tyr Asn Cys Ala Phe Ile 
        515                 520                 525             
Trp Gln Lys Asp Gly Val Lys Lys Leu Asn Leu Tyr Leu Ile Ile Asn 
    530                 535                 540                 
Tyr Phe Lys Gly Gly Lys Leu Arg Phe Lys Lys Ile Lys Pro Glu Ala 
545                 550                 555                 560 
Phe Glu Ala Asn Arg Phe Tyr Thr Val Ile Asn Lys Lys Ser Gly Glu 
                565                 570                 575     
Ile Val Pro Met Glu Val Asn Phe Asn Phe Asp Asp Pro Asn Leu Ile 
            580                 585                 590         
Ile Leu Pro Leu Ala Phe Gly Lys Arg Gln Gly Arg Glu Phe Ile Trp 
        595                 600                 605             
Asn Asp Leu Leu Ser Leu Glu Thr Gly Ser Leu Lys Leu Ala Asn Gly 
    610                 615                 620                 
Arg Val Ile Glu Lys Thr Leu Tyr Asn Arg Arg Thr Arg Gln Asp Glu 
625                 630                 635                 640 
Pro Ala Leu Phe Val Ala Leu Thr Phe Glu Arg Arg Glu Val Leu Asp 
                645                 650                 655     
Ser Ser Asn Ile Lys Pro Met Asn Leu Ile Gly Ile Asp Arg Gly Glu 
            660                 665                 670         
Asn Ile Pro Ala Val Ile Ala Leu Thr Asp Pro Glu Gly Cys Pro Leu 
        675                 680                 685             
Ser Arg Phe Lys Asp Ser Leu Gly Asn Pro Thr His Ile Leu Arg Ile 
    690                 695                 700                 
Gly Glu Ser Tyr Lys Glu Lys Gln Arg Thr Ile Gln Ala Ala Lys Glu 
705                 710                 715                 720 
Val Glu Gln Arg Arg Ala Gly Gly Tyr Ser Arg Lys Tyr Ala Ser Lys 
                725                 730                 735     
Ala Lys Asn Leu Ala Asp Asp Met Val Arg Asn Thr Ala Arg Asp Leu 
            740                 745                 750         
Leu Tyr Tyr Ala Val Thr Gln Asp Ala Met Leu Ile Phe Glu Asn Leu 
        755                 760                 765             
Ser Arg Gly Phe Gly Arg Gln Gly Lys Arg Thr Phe Met Ala Glu Arg 
    770                 775                 780                 
Gln Tyr Thr Arg Met Glu Asp Trp Leu Thr Ala Lys Leu Ala Tyr Glu 
785                 790                 795                 800 
Gly Leu Pro Ser Lys Thr Tyr Leu Ser Lys Thr Leu Ala Gln Tyr Thr 
                805                 810                 815     
Ser Lys Thr Cys Ser Asn Cys Gly Phe Thr Ile Thr Ser Ala Asp Tyr 
            820                 825                 830         
Asp Arg Val Leu Glu Lys Leu Lys Lys Thr Ala Thr Gly Trp Met Thr 
        835                 840                 845             
Thr Ile Asn Gly Lys Glu Leu Lys Val Glu Gly Gln Ile Thr Tyr Tyr 
    850                 855                 860                 
Asn Arg Tyr Lys Arg Gln Asn Val Val Lys Asp Leu Ser Val Glu Leu 
865                 870                 875                 880 
Asp Arg Leu Ser Glu Glu Ser Val Asn Asn Asp Ile Ser Ser Trp Thr 
                885                 890                 895     
Lys Gly Arg Ser Gly Glu Ala Leu Ser Leu Leu Lys Lys Arg Phe Ser 
            900                 905                 910         
His Arg Pro Val Gln Glu Lys Phe Val Cys Leu Asn Cys Gly Phe Glu 
        915                 920                 925             
Thr His Ala Asp Glu Gln Ala Ala Leu Asn Ile Ala Arg Ser Trp Leu 
    930                 935                 940                 
Phe Leu Arg Ser Gln Glu Tyr Lys Lys Tyr Gln Thr Asn Lys Thr Thr 
945                 950                 955                 960 
Gly Asn Thr Asp Lys Arg Ala Phe Val Glu Thr Trp Gln Ser Phe Tyr 
                965                 970                 975     
Arg Lys Lys Leu Lys Glu Val Trp Lys Pro Ala Val Thr Ser 
            980                 985                 990 

<210> 71
<211> 1782
<212> DNA
<213> Artificial Sequence


<220> 
<223> hyperactive PiggyBac (PB) transposase nucleic acid

<400> 71
atgggcagca gcctggacga cgagcacatc ctgagcgccc tgctgcagag cgacgacgag      60

ctggtcggcg aggacagcga cagcgaggtg agcgaccacg tgagcgagga cgacgtgcag     120

tccgacaccg aggaggcctt catcgacgag gtgcacgagg tgcagcctac cagcagcggc     180

tccgagatcc tggacgagca gaacgtgatc gagcagcccg gcagctccct ggccagcaac     240

aggatcctga ccctgcccca gaggaccatc aggggcaaga acaagcactg ctggtccacc     300

tccaagccca ccaggcggag cagggtgtcc gccctgaaca tcgtgagaag ccagaggggc     360

cccaccagga tgtgcaggaa catctacgac cccctgctgt gcttcaagct gttcttcacc     420

gacgagatca tcagcgagat cgtgaagtgg accaacgccg agatcagcct gaagaggcgg     480

gagagcatga cctccgccac cttcagggac accaacgagg acgagatcta cgccttcttc     540

ggcatcctgg tgatgaccgc cgtgaggaag gacaaccaca tgagcaccga cgacctgttc     600

gacagatccc tgagcatggt gtacgtgagc gtgatgagca gggacagatt cgacttcctg     660

atcagatgcc tgaggatgga cgacaagagc atcaggccca ccctgcggga gaacgacgtg     720

ttcacccccg tgagaaagat ctgggacctg ttcatccacc agtgcatcca gaactacacc     780

cctggcgccc acctgaccat cgacgagcag ctgctgggct tcaggggcag gtgccccttc     840

agggtctata tccccaacaa gcccagcaag tacggcatca agatcctgat gatgtgcgac     900

agcggcacca agtacatgat caacggcatg ccctacctgg gcaggggcac ccagaccaac     960

ggcgtgcccc tgggcgagta ctacgtgaag gagctgtcca agcccgtcca cggcagctgc    1020

agaaacatca cctgcgacaa ctggttcacc agcatccccc tggccaagaa cctgctgcag    1080

gagccctaca agctgaccat cgtgggcacc gtgagaagca acaagagaga gatccccgag    1140

gtcctgaaga acagcaggtc caggcccgtg ggcaccagca tgttctgctt cgacggcccc    1200

ctgaccctgg tgtcctacaa gcccaagccc gccaagatgg tgtacctgct gtccagctgc    1260

gacgaggacg ccagcatcaa cgagagcacc ggcaagcccc agatggtgat gtactacaac    1320

cagaccaagg gcggcgtgga caccctggac cagatgtgca gcgtgatgac ctgcagcaga    1380

aagaccaaca ggtggcccat ggccctgctg tacggcatga tcaacatcgc ctgcatcaac    1440

agcttcatca tctacagcca caacgtgagc agcaagggcg agaaggtgca gagccggaaa    1500

aagttcatgc ggaacctgta catgggcctg acctccagct tcatgaggaa gaggctggag    1560

gcccccaccc tgaagagata cctgagggac aacatcagca acatcctgcc caaagaggtg    1620

cccggcacca gcgacgacag caccgaggag cccgtgatga agaagaggac ctactgcacc    1680

tactgtccca gcaagatcag aagaaaggcc agcgccagct gcaagaagtg taagaaggtc    1740

atctgccggg agcacaacat cgacatgtgc cagagctgtt tc                       1782


<210> 72
<211> 1020
<212> DNA
<213> Artificial Sequence


<220> 
<223> hyperactive Sleeping Beauty (SB100) transposase nucleic acid

<400> 72
atgggaaaat caaaagaaat cagccaagac ctcagaaaaa gaattgtaga cctccacaag      60

tctggttcat ccttgggagc aatttccaaa cgcctggcgg taccacgttc atctgtacaa     120

acaatagtac gcaagtataa acaccatggg accacgcagc cgtcataccg ctcaggaagg     180

agacgcgttc tgtctcctag agatgaacgt actttggtgc gaaaagtgca aatcaatccc     240

agaacaacag caaaggacct tgtgaagatg ctggaggaaa caggtacaaa agtatctata     300

tccacagtaa aacgagtcct atatcgacat aacctgaaag gccactcagc aaggaagaag     360

ccactgctcc aaaaccgaca taagaaagcc agactacggt ttgcaactgc acatggggac     420

aaagatcgta ctttttggag aaatgtcctc tggtctgatg aaacaaaaat agaactgttt     480

ggccataatg accatcgtta tgtttggagg aagaaggggg aggcttgcaa gccgaagaac     540

accatcccaa ccgtgaagca cgggggtggc agcatcatgt tgtgggggtg ctttgctgca     600

ggagggactg gtgcacttca caaaatagat ggcatcatgg acgccgtgca gtatgtggat     660

atattgaagc aacatctcaa gacatcagtc aggaagttaa agcttggtcg caaatgggtc     720

ttccaacacg acaatgaccc caagcatact tccaaagttg tggcaaaatg gcttaaggac     780

aacaaagtca aggtattgga gtggccatca caaagccctg acctcaatcc tatagaaaat     840

ttgtgggcag aactgaaaaa gcgtgtgcga gcaaggaggc ctacaaacct gactcagtta     900

caccagctct gtcaggagga atgggccaaa attcacccaa attattgtgg gaagcttgtg     960

gaaggctacc cgaaacgttt gacccaagtt aaacaattta aaggcaatgc taccaaatac    1020


<210> 73
<211> 340
<212> PRT
<213> Artificial Sequence


<220> 
<223> hyperactive Sleeping Beauty (SB100) transposase amino acid

<400> 73
Met Gly Lys Ser Lys Glu Ile Ser Gln Asp Leu Arg Lys Arg Ile Val 
1               5                   10                  15      
Asp Leu His Lys Ser Gly Ser Ser Leu Gly Ala Ile Ser Lys Arg Leu 
            20                  25                  30          
Ala Val Pro Arg Ser Ser Val Gln Thr Ile Val Arg Lys Tyr Lys His 
        35                  40                  45              
His Gly Thr Thr Gln Pro Ser Tyr Arg Ser Gly Arg Arg Arg Val Leu 
    50                  55                  60                  
Ser Pro Arg Asp Glu Arg Thr Leu Val Arg Lys Val Gln Ile Asn Pro 
65                  70                  75                  80  
Arg Thr Thr Ala Lys Asp Leu Val Lys Met Leu Glu Glu Thr Gly Thr 
                85                  90                  95      
Lys Val Ser Ile Ser Thr Val Lys Arg Val Leu Tyr Arg His Asn Leu 
            100                 105                 110         
Lys Gly His Ser Ala Arg Lys Lys Pro Leu Leu Gln Asn Arg His Lys 
        115                 120                 125             
Lys Ala Arg Leu Arg Phe Ala Thr Ala His Gly Asp Lys Asp Arg Thr 
    130                 135                 140                 
Phe Trp Arg Asn Val Leu Trp Ser Asp Glu Thr Lys Ile Glu Leu Phe 
145                 150                 155                 160 
Gly His Asn Asp His Arg Tyr Val Trp Arg Lys Lys Gly Glu Ala Cys 
                165                 170                 175     
Lys Pro Lys Asn Thr Ile Pro Thr Val Lys His Gly Gly Gly Ser Ile 
            180                 185                 190         
Met Leu Trp Gly Cys Phe Ala Ala Gly Gly Thr Gly Ala Leu His Lys 
        195                 200                 205             
Ile Asp Gly Ile Met Asp Ala Val Gln Tyr Val Asp Ile Leu Lys Gln 
    210                 215                 220                 
His Leu Lys Thr Ser Val Arg Lys Leu Lys Leu Gly Arg Lys Trp Val 
225                 230                 235                 240 
Phe Gln His Asp Asn Asp Pro Lys His Thr Ser Lys Val Val Ala Lys 
                245                 250                 255     
Trp Leu Lys Asp Asn Lys Val Lys Val Leu Glu Trp Pro Ser Gln Ser 
            260                 265                 270         
Pro Asp Leu Asn Pro Ile Glu Asn Leu Trp Ala Glu Leu Lys Lys Arg 
        275                 280                 285             
Val Arg Ala Arg Arg Pro Thr Asn Leu Thr Gln Leu His Gln Leu Cys 
    290                 295                 300                 
Gln Glu Glu Trp Ala Lys Ile His Pro Asn Tyr Cys Gly Lys Leu Val 
305                 310                 315                 320 
Glu Gly Tyr Pro Lys Arg Leu Thr Gln Val Lys Gln Phe Lys Gly Asn 
                325                 330                 335     
Ala Thr Lys Tyr 
            340 

<210> 74
<211> 3123
<212> PRT
<213> homo sapiens


<400> 74
Met Pro Gly Ala Ala Gly Val Leu Leu Leu Leu Leu Leu Ser Gly Gly 
1               5                   10                  15      
Leu Gly Gly Val Gln Ala Gln Arg Pro Gln Gln Gln Arg Gln Ser Gln 
            20                  25                  30          
Ala His Gln Gln Arg Gly Leu Phe Pro Ala Val Leu Asn Leu Ala Ser 
        35                  40                  45              
Asn Ala Leu Ile Thr Thr Asn Ala Thr Cys Gly Glu Lys Gly Pro Glu 
    50                  55                  60                  
Met Tyr Cys Lys Leu Val Glu His Val Pro Gly Gln Pro Val Arg Asn 
65                  70                  75                  80  
Pro Gln Cys Arg Ile Cys Asn Gln Asn Ser Ser Asn Pro Asn Gln Arg 
                85                  90                  95      
His Pro Ile Thr Asn Ala Ile Asp Gly Lys Asn Thr Trp Trp Gln Ser 
            100                 105                 110         
Pro Ser Ile Lys Asn Gly Ile Glu Tyr His Tyr Val Thr Ile Thr Leu 
        115                 120                 125             
Asp Leu Gln Gln Val Phe Gln Ile Ala Tyr Val Ile Val Lys Ala Ala 
    130                 135                 140                 
Asn Ser Pro Arg Pro Gly Asn Trp Ile Leu Glu Arg Ser Leu Asp Asp 
145                 150                 155                 160 
Val Glu Tyr Lys Pro Trp Gln Tyr His Ala Val Thr Asp Thr Glu Cys 
                165                 170                 175     
Leu Thr Leu Tyr Asn Ile Tyr Pro Arg Thr Gly Pro Pro Ser Tyr Ala 
            180                 185                 190         
Lys Asp Asp Glu Val Ile Cys Thr Ser Phe Tyr Ser Lys Ile His Pro 
        195                 200                 205             
Leu Glu Asn Gly Glu Ile His Ile Ser Leu Ile Asn Gly Arg Pro Ser 
    210                 215                 220                 
Ala Asp Asp Pro Ser Pro Glu Leu Leu Glu Phe Thr Ser Ala Arg Tyr 
225                 230                 235                 240 
Ile Arg Leu Arg Phe Gln Arg Ile Arg Thr Leu Asn Ala Asp Leu Met 
                245                 250                 255     
Met Phe Ala His Lys Asp Pro Arg Glu Ile Asp Pro Ile Val Thr Arg 
            260                 265                 270         
Arg Tyr Tyr Tyr Ser Val Lys Asp Ile Ser Val Gly Gly Met Cys Ile 
        275                 280                 285             
Cys Tyr Gly His Ala Arg Ala Cys Pro Leu Asp Pro Ala Thr Asn Lys 
    290                 295                 300                 
Ser Arg Cys Glu Cys Glu His Asn Thr Cys Gly Asp Ser Cys Asp Gln 
305                 310                 315                 320 
Cys Cys Pro Gly Phe His Gln Lys Pro Trp Arg Ala Gly Thr Phe Leu 
                325                 330                 335     
Thr Lys Thr Glu Cys Glu Ala Cys Asn Cys His Gly Lys Ala Glu Glu 
            340                 345                 350         
Cys Tyr Tyr Asp Glu Asn Val Ala Arg Arg Asn Leu Ser Leu Asn Ile 
        355                 360                 365             
Arg Gly Lys Tyr Ile Gly Gly Gly Val Cys Ile Asn Cys Thr Gln Asn 
    370                 375                 380                 
Thr Ala Gly Ile Asn Cys Glu Thr Cys Thr Asp Gly Phe Phe Arg Pro 
385                 390                 395                 400 
Lys Gly Val Ser Pro Asn Tyr Pro Arg Pro Cys Gln Pro Cys His Cys 
                405                 410                 415     
Asp Pro Ile Gly Ser Leu Asn Glu Val Cys Val Lys Asp Glu Lys His 
            420                 425                 430         
Ala Arg Arg Gly Leu Ala Pro Gly Ser Cys His Cys Lys Thr Gly Phe 
        435                 440                 445             
Gly Gly Val Ser Cys Asp Arg Cys Ala Arg Gly Tyr Thr Gly Tyr Pro 
    450                 455                 460                 
Asp Cys Lys Ala Cys Asn Cys Ser Gly Leu Gly Ser Lys Asn Glu Asp 
465                 470                 475                 480 
Pro Cys Phe Gly Pro Cys Ile Cys Lys Glu Asn Val Glu Gly Gly Asp 
                485                 490                 495     
Cys Ser Arg Cys Lys Ser Gly Phe Phe Asn Leu Gln Glu Asp Asn Trp 
            500                 505                 510         
Lys Gly Cys Asp Glu Cys Phe Cys Ser Gly Val Ser Asn Arg Cys Gln 
        515                 520                 525             
Ser Ser Tyr Trp Thr Tyr Gly Lys Ile Gln Asp Met Ser Gly Trp Tyr 
    530                 535                 540                 
Leu Thr Asp Leu Pro Gly Arg Ile Arg Val Ala Pro Gln Gln Asp Asp 
545                 550                 555                 560 
Leu Asp Ser Pro Gln Gln Ile Ser Ile Ser Asn Ala Glu Ala Arg Gln 
                565                 570                 575     
Ala Leu Pro His Ser Tyr Tyr Trp Ser Ala Pro Ala Pro Tyr Leu Gly 
            580                 585                 590         
Asn Lys Leu Pro Ala Val Gly Gly Gln Leu Thr Phe Thr Ile Ser Tyr 
        595                 600                 605             
Asp Leu Glu Glu Glu Glu Glu Asp Thr Glu Arg Val Leu Gln Leu Met 
    610                 615                 620                 
Ile Ile Leu Glu Gly Asn Asp Leu Ser Ile Ser Thr Ala Gln Asp Glu 
625                 630                 635                 640 
Val Tyr Leu His Pro Ser Glu Glu His Thr Asn Val Leu Leu Leu Lys 
                645                 650                 655     
Glu Glu Ser Phe Thr Ile His Gly Thr His Phe Pro Val Arg Arg Lys 
            660                 665                 670         
Glu Phe Met Thr Val Leu Ala Asn Leu Lys Arg Val Leu Leu Gln Ile 
        675                 680                 685             
Thr Tyr Ser Phe Gly Met Asp Ala Ile Phe Arg Leu Ser Ser Val Asn 
    690                 695                 700                 
Leu Glu Ser Ala Val Ser Tyr Pro Thr Asp Gly Ser Ile Ala Ala Ala 
705                 710                 715                 720 
Val Glu Val Cys Gln Cys Pro Pro Gly Tyr Thr Gly Ser Ser Cys Glu 
                725                 730                 735     
Ser Cys Trp Pro Arg His Arg Arg Val Asn Gly Thr Ile Phe Gly Gly 
            740                 745                 750         
Ile Cys Glu Pro Cys Gln Cys Phe Gly His Ala Glu Ser Cys Asp Asp 
        755                 760                 765             
Val Thr Gly Glu Cys Leu Asn Cys Lys Asp His Thr Gly Gly Pro Tyr 
    770                 775                 780                 
Cys Asp Lys Cys Leu Pro Gly Phe Tyr Gly Glu Pro Thr Lys Gly Thr 
785                 790                 795                 800 
Ser Glu Asp Cys Gln Pro Cys Ala Cys Pro Leu Asn Ile Pro Ser Asn 
                805                 810                 815     
Asn Phe Ser Pro Thr Cys His Leu Asp Arg Ser Leu Gly Leu Ile Cys 
            820                 825                 830         
Asp Gly Cys Pro Val Gly Tyr Thr Gly Pro Arg Cys Glu Arg Cys Ala 
        835                 840                 845             
Glu Gly Tyr Phe Gly Gln Pro Ser Val Pro Gly Gly Ser Cys Gln Pro 
    850                 855                 860                 
Cys Gln Cys Asn Asp Asn Leu Asp Phe Ser Ile Pro Gly Ser Cys Asp 
865                 870                 875                 880 
Ser Leu Ser Gly Ser Cys Leu Ile Cys Lys Pro Gly Thr Thr Gly Arg 
                885                 890                 895     
Tyr Cys Glu Leu Cys Ala Asp Gly Tyr Phe Gly Asp Ala Val Asp Ala 
            900                 905                 910         
Lys Asn Cys Gln Pro Cys Arg Cys Asn Ala Gly Gly Ser Phe Ser Glu 
        915                 920                 925             
Val Cys His Ser Gln Thr Gly Gln Cys Glu Cys Arg Ala Asn Val Gln 
    930                 935                 940                 
Gly Gln Arg Cys Asp Lys Cys Lys Ala Gly Thr Phe Gly Leu Gln Ser 
945                 950                 955                 960 
Ala Arg Gly Cys Val Pro Cys Asn Cys Asn Ser Phe Gly Ser Lys Ser 
                965                 970                 975     
Phe Asp Cys Glu Glu Ser Gly Gln Cys Trp Cys Gln Pro Gly Val Thr 
            980                 985                 990         
Gly Lys Lys Cys Asp Arg Cys Ala His Gly Tyr Phe Asn Phe Gln Glu 
        995                 1000                1005            
Gly Gly Cys Thr Ala Cys Glu Cys Ser His Leu Gly Asn Asn Cys Asp 
    1010                1015                1020                
Pro Lys Thr Gly Arg Cys Ile Cys Pro Pro Asn Thr Ile Gly Glu Lys 
1025                1030                1035                1040
Cys Ser Lys Cys Ala Pro Asn Thr Trp Gly His Ser Ile Thr Thr Gly 
                1045                1050                1055    
Cys Lys Ala Cys Asn Cys Ser Thr Val Gly Ser Leu Asp Phe Gln Cys 
            1060                1065                1070        
Asn Val Asn Thr Gly Gln Cys Asn Cys His Pro Lys Phe Ser Gly Ala 
        1075                1080                1085            
Lys Cys Thr Glu Cys Ser Arg Gly His Trp Asn Tyr Pro Arg Cys Asn 
    1090                1095                1100                
Leu Cys Asp Cys Phe Leu Pro Gly Thr Asp Ala Thr Thr Cys Asp Ser 
1105                1110                1115                1120
Glu Thr Lys Lys Cys Ser Cys Ser Asp Gln Thr Gly Gln Cys Thr Cys 
                1125                1130                1135    
Lys Val Asn Val Glu Gly Ile His Cys Asp Arg Cys Arg Pro Gly Lys 
            1140                1145                1150        
Phe Gly Leu Asp Ala Lys Asn Pro Leu Gly Cys Ser Ser Cys Tyr Cys 
        1155                1160                1165            
Phe Gly Thr Thr Thr Gln Cys Ser Glu Ala Lys Gly Leu Ile Arg Thr 
    1170                1175                1180                
Trp Val Thr Leu Lys Ala Glu Gln Thr Ile Leu Pro Leu Val Asp Glu 
1185                1190                1195                1200
Ala Leu Gln His Thr Thr Thr Lys Gly Ile Val Phe Gln His Pro Glu 
                1205                1210                1215    
Ile Val Ala His Met Asp Leu Met Arg Glu Asp Leu His Leu Glu Pro 
            1220                1225                1230        
Phe Tyr Trp Lys Leu Pro Glu Gln Phe Glu Gly Lys Lys Leu Met Ala 
        1235                1240                1245            
Tyr Gly Gly Lys Leu Lys Tyr Ala Ile Tyr Phe Glu Ala Arg Glu Glu 
    1250                1255                1260                
Thr Gly Phe Ser Thr Tyr Asn Pro Gln Val Ile Ile Arg Gly Gly Thr 
1265                1270                1275                1280
Pro Thr His Ala Arg Ile Ile Val Arg His Met Ala Ala Pro Leu Ile 
                1285                1290                1295    
Gly Gln Leu Thr Arg His Glu Ile Glu Met Thr Glu Lys Glu Trp Lys 
            1300                1305                1310        
Tyr Tyr Gly Asp Asp Pro Arg Val His Arg Thr Val Thr Arg Glu Asp 
        1315                1320                1325            
Phe Leu Asp Ile Leu Tyr Asp Ile His Tyr Ile Leu Ile Lys Ala Thr 
    1330                1335                1340                
Tyr Gly Asn Phe Met Arg Gln Ser Arg Ile Ser Glu Ile Ser Met Glu 
1345                1350                1355                1360
Val Ala Glu Gln Gly Arg Gly Thr Thr Met Thr Pro Pro Ala Asp Leu 
                1365                1370                1375    
Ile Glu Lys Cys Asp Cys Pro Leu Gly Tyr Ser Gly Leu Ser Cys Glu 
            1380                1385                1390        
Ala Cys Leu Pro Gly Phe Tyr Arg Leu Arg Ser Gln Pro Gly Gly Arg 
        1395                1400                1405            
Thr Pro Gly Pro Thr Leu Gly Thr Cys Val Pro Cys Gln Cys Asn Gly 
    1410                1415                1420                
His Ser Ser Leu Cys Asp Pro Glu Thr Ser Ile Cys Gln Asn Cys Gln 
1425                1430                1435                1440
His His Thr Ala Gly Asp Phe Cys Glu Arg Cys Ala Leu Gly Tyr Tyr 
                1445                1450                1455    
Gly Ile Val Lys Gly Leu Pro Asn Asp Cys Gln Gln Cys Ala Cys Pro 
            1460                1465                1470        
Leu Ile Ser Ser Ser Asn Asn Phe Ser Pro Ser Cys Val Ala Glu Gly 
        1475                1480                1485            
Leu Asp Asp Tyr Arg Cys Thr Ala Cys Pro Arg Gly Tyr Glu Gly Gln 
    1490                1495                1500                
Tyr Cys Glu Arg Cys Ala Pro Gly Tyr Thr Gly Ser Pro Gly Asn Pro 
1505                1510                1515                1520
Gly Gly Ser Cys Gln Glu Cys Glu Cys Asp Pro Tyr Gly Ser Leu Pro 
                1525                1530                1535    
Val Pro Cys Asp Pro Val Thr Gly Phe Cys Thr Cys Arg Pro Gly Ala 
            1540                1545                1550        
Thr Gly Arg Lys Cys Asp Gly Cys Lys His Trp His Ala Arg Glu Gly 
        1555                1560                1565            
Trp Glu Cys Val Phe Cys Gly Asp Glu Cys Thr Gly Leu Leu Leu Gly 
    1570                1575                1580                
Asp Leu Ala Arg Leu Glu Gln Met Val Met Ser Ile Asn Leu Thr Gly 
1585                1590                1595                1600
Pro Leu Pro Ala Pro Tyr Lys Met Leu Tyr Gly Leu Glu Asn Met Thr 
                1605                1610                1615    
Gln Glu Leu Lys His Leu Leu Ser Pro Gln Arg Ala Pro Glu Arg Leu 
            1620                1625                1630        
Ile Gln Leu Ala Glu Gly Asn Leu Asn Thr Leu Val Thr Glu Met Asn 
        1635                1640                1645            
Glu Leu Leu Thr Arg Ala Thr Lys Val Thr Ala Asp Gly Glu Gln Thr 
    1650                1655                1660                
Gly Gln Asp Ala Glu Arg Thr Asn Thr Arg Ala Lys Ser Leu Gly Glu 
1665                1670                1675                1680
Phe Ile Lys Glu Leu Ala Arg Asp Ala Glu Ala Val Asn Glu Lys Ala 
                1685                1690                1695    
Ile Lys Leu Asn Glu Thr Leu Gly Thr Arg Asp Glu Ala Phe Glu Arg 
            1700                1705                1710        
Asn Leu Glu Gly Leu Gln Lys Glu Ile Asp Gln Met Ile Lys Glu Leu 
        1715                1720                1725            
Arg Arg Lys Asn Leu Glu Thr Gln Lys Glu Ile Ala Glu Asp Glu Leu 
    1730                1735                1740                
Val Ala Ala Glu Ala Leu Leu Lys Lys Val Lys Lys Leu Phe Gly Glu 
1745                1750                1755                1760
Ser Arg Gly Glu Asn Glu Glu Met Glu Lys Asp Leu Arg Glu Lys Leu 
                1765                1770                1775    
Ala Asp Tyr Lys Asn Lys Val Asp Asp Ala Trp Asp Leu Leu Arg Glu 
            1780                1785                1790        
Ala Thr Asp Lys Ile Arg Glu Ala Asn Arg Leu Phe Ala Val Asn Gln 
        1795                1800                1805            
Lys Asn Met Thr Ala Leu Glu Lys Lys Lys Glu Ala Val Glu Ser Gly 
    1810                1815                1820                
Lys Arg Gln Ile Glu Asn Thr Leu Lys Glu Gly Asn Asp Ile Leu Asp 
1825                1830                1835                1840
Glu Ala Asn Arg Leu Ala Asp Glu Ile Asn Ser Ile Ile Asp Tyr Val 
                1845                1850                1855    
Glu Asp Ile Gln Thr Lys Leu Pro Pro Met Ser Glu Glu Leu Asn Asp 
            1860                1865                1870        
Lys Ile Asp Asp Leu Ser Gln Glu Ile Lys Asp Arg Lys Leu Ala Glu 
        1875                1880                1885            
Lys Val Ser Gln Ala Glu Ser His Ala Ala Gln Leu Asn Asp Ser Ser 
    1890                1895                1900                
Ala Val Leu Asp Gly Ile Leu Asp Glu Ala Lys Asn Ile Ser Phe Asn 
1905                1910                1915                1920
Ala Thr Ala Ala Phe Lys Ala Tyr Ser Asn Ile Lys Asp Tyr Ile Asp 
                1925                1930                1935    
Glu Ala Glu Lys Val Ala Lys Glu Ala Lys Asp Leu Ala His Glu Ala 
            1940                1945                1950        
Thr Lys Leu Ala Thr Gly Pro Arg Gly Leu Leu Lys Glu Asp Ala Lys 
        1955                1960                1965            
Gly Cys Leu Gln Lys Ser Phe Arg Ile Leu Asn Glu Ala Lys Lys Leu 
    1970                1975                1980                
Ala Asn Asp Val Lys Glu Asn Glu Asp His Leu Asn Gly Leu Lys Thr 
1985                1990                1995                2000
Arg Ile Glu Asn Ala Asp Ala Arg Asn Gly Asp Leu Leu Arg Thr Leu 
                2005                2010                2015    
Asn Asp Thr Leu Gly Lys Leu Ser Ala Ile Pro Asn Asp Thr Ala Ala 
            2020                2025                2030        
Lys Leu Gln Ala Val Lys Asp Lys Ala Arg Gln Ala Asn Asp Thr Ala 
        2035                2040                2045            
Lys Asp Val Leu Ala Gln Ile Thr Glu Leu His Gln Asn Leu Asp Gly 
    2050                2055                2060                
Leu Lys Lys Asn Tyr Asn Lys Leu Ala Asp Ser Val Ala Lys Thr Asn 
2065                2070                2075                2080
Ala Val Val Lys Asp Pro Ser Lys Asn Lys Ile Ile Ala Asp Ala Asp 
                2085                2090                2095    
Ala Thr Val Lys Asn Leu Glu Gln Glu Ala Asp Arg Leu Ile Asp Lys 
            2100                2105                2110        
Leu Lys Pro Ile Lys Glu Leu Glu Asp Asn Leu Lys Lys Asn Ile Ser 
        2115                2120                2125            
Glu Ile Lys Glu Leu Ile Asn Gln Ala Arg Lys Gln Ala Asn Ser Ile 
    2130                2135                2140                
Lys Val Ser Val Ser Ser Gly Gly Asp Cys Ile Arg Thr Tyr Lys Pro 
2145                2150                2155                2160
Glu Ile Lys Lys Gly Ser Tyr Asn Asn Ile Val Val Asn Val Lys Thr 
                2165                2170                2175    
Ala Val Ala Asp Asn Leu Leu Phe Tyr Leu Gly Ser Ala Lys Phe Ile 
            2180                2185                2190        
Asp Phe Leu Ala Ile Glu Met Arg Lys Gly Lys Val Ser Phe Leu Trp 
        2195                2200                2205            
Asp Val Gly Ser Gly Val Gly Arg Val Glu Tyr Pro Asp Leu Thr Ile 
    2210                2215                2220                
Asp Asp Ser Tyr Trp Tyr Arg Ile Val Ala Ser Arg Thr Gly Arg Asn 
2225                2230                2235                2240
Gly Thr Ile Ser Val Arg Ala Leu Asp Gly Pro Lys Ala Ser Ile Val 
                2245                2250                2255    
Pro Ser Thr His His Ser Thr Ser Pro Pro Gly Tyr Thr Ile Leu Asp 
            2260                2265                2270        
Val Asp Ala Asn Ala Met Leu Phe Val Gly Gly Leu Thr Gly Lys Leu 
        2275                2280                2285            
Lys Lys Ala Asp Ala Val Arg Val Ile Thr Phe Thr Gly Cys Met Gly 
    2290                2295                2300                
Glu Thr Tyr Phe Asp Asn Lys Pro Ile Gly Leu Trp Asn Phe Arg Glu 
2305                2310                2315                2320
Lys Glu Gly Asp Cys Lys Gly Cys Thr Val Ser Pro Gln Val Glu Asp 
                2325                2330                2335    
Ser Glu Gly Thr Ile Gln Phe Asp Gly Glu Gly Tyr Ala Leu Val Ser 
            2340                2345                2350        
Arg Pro Ile Arg Trp Tyr Pro Asn Ile Ser Thr Val Met Phe Lys Phe 
        2355                2360                2365            
Arg Thr Phe Ser Ser Ser Ala Leu Leu Met Tyr Leu Ala Thr Arg Asp 
    2370                2375                2380                
Leu Arg Asp Phe Met Ser Val Glu Leu Thr Asp Gly His Ile Lys Val 
2385                2390                2395                2400
Ser Tyr Asp Leu Gly Ser Gly Met Ala Ser Val Val Ser Asn Gln Asn 
                2405                2410                2415    
His Asn Asp Gly Lys Trp Lys Ser Phe Thr Leu Ser Arg Ile Gln Lys 
            2420                2425                2430        
Gln Ala Asn Ile Ser Ile Val Asp Ile Asp Thr Asn Gln Glu Glu Asn 
        2435                2440                2445            
Ile Ala Thr Ser Ser Ser Gly Asn Asn Phe Gly Leu Asp Leu Lys Ala 
    2450                2455                2460                
Asp Asp Lys Ile Tyr Phe Gly Gly Leu Pro Thr Leu Arg Asn Leu Ser 
2465                2470                2475                2480
Met Lys Ala Arg Pro Glu Val Asn Leu Lys Lys Tyr Ser Gly Cys Leu 
                2485                2490                2495    
Lys Asp Ile Glu Ile Ser Arg Thr Pro Tyr Asn Ile Leu Ser Ser Pro 
            2500                2505                2510        
Asp Tyr Val Gly Val Thr Lys Gly Cys Ser Leu Glu Asn Val Tyr Thr 
        2515                2520                2525            
Val Ser Phe Pro Lys Pro Gly Phe Val Glu Leu Ser Pro Val Pro Ile 
    2530                2535                2540                
Asp Val Gly Thr Glu Ile Asn Leu Ser Phe Ser Thr Lys Asn Glu Ser 
2545                2550                2555                2560
Gly Ile Ile Leu Leu Gly Ser Gly Gly Thr Pro Ala Pro Pro Arg Arg 
                2565                2570                2575    
Lys Arg Arg Gln Thr Gly Gln Ala Tyr Tyr Val Ile Leu Leu Asn Arg 
            2580                2585                2590        
Gly Arg Leu Glu Val His Leu Ser Thr Gly Ala Arg Thr Met Arg Lys 
        2595                2600                2605            
Ile Val Ile Arg Pro Glu Pro Asn Leu Phe His Asp Gly Arg Glu His 
    2610                2615                2620                
Ser Val His Val Glu Arg Thr Arg Gly Ile Phe Thr Val Gln Val Asp 
2625                2630                2635                2640
Glu Asn Arg Arg Tyr Met Gln Asn Leu Thr Val Glu Gln Pro Ile Glu 
                2645                2650                2655    
Val Lys Lys Leu Phe Val Gly Gly Ala Pro Pro Glu Phe Gln Pro Ser 
            2660                2665                2670        
Pro Leu Arg Asn Ile Pro Pro Phe Glu Gly Cys Ile Trp Asn Leu Val 
        2675                2680                2685            
Ile Asn Ser Val Pro Met Asp Phe Ala Arg Pro Val Ser Phe Lys Asn 
    2690                2695                2700                
Ala Asp Ile Gly Arg Cys Ala His Gln Lys Leu Arg Glu Asp Glu Asp 
2705                2710                2715                2720
Gly Ala Ala Pro Ala Glu Ile Val Ile Gln Pro Glu Pro Val Pro Thr 
                2725                2730                2735    
Pro Ala Phe Pro Thr Pro Thr Pro Val Leu Thr His Gly Pro Cys Ala 
            2740                2745                2750        
Ala Glu Ser Glu Pro Ala Leu Leu Ile Gly Ser Lys Gln Phe Gly Leu 
        2755                2760                2765            
Ser Arg Asn Ser His Ile Ala Ile Ala Phe Asp Asp Thr Lys Val Lys 
    2770                2775                2780                
Asn Arg Leu Thr Ile Glu Leu Glu Val Arg Thr Glu Ala Glu Ser Gly 
2785                2790                2795                2800
Leu Leu Phe Tyr Met Ala Arg Ile Asn His Ala Asp Phe Ala Thr Val 
                2805                2810                2815    
Gln Leu Arg Asn Gly Leu Pro Tyr Phe Ser Tyr Asp Leu Gly Ser Gly 
            2820                2825                2830        
Asp Thr His Thr Met Ile Pro Thr Lys Ile Asn Asp Gly Gln Trp His 
        2835                2840                2845            
Lys Ile Lys Ile Met Arg Ser Lys Gln Glu Gly Ile Leu Tyr Val Asp 
    2850                2855                2860                
Gly Ala Ser Asn Arg Thr Ile Ser Pro Lys Lys Ala Asp Ile Leu Asp 
2865                2870                2875                2880
Val Val Gly Met Leu Tyr Val Gly Gly Leu Pro Ile Asn Tyr Thr Thr 
                2885                2890                2895    
Arg Arg Ile Gly Pro Val Thr Tyr Ser Ile Asp Gly Cys Val Arg Asn 
            2900                2905                2910        
Leu His Met Ala Glu Ala Pro Ala Asp Leu Glu Gln Pro Thr Ser Ser 
        2915                2920                2925            
Phe His Val Gly Thr Cys Phe Ala Asn Ala Gln Arg Gly Thr Tyr Phe 
    2930                2935                2940                
Asp Gly Thr Gly Phe Ala Lys Ala Val Gly Gly Phe Lys Val Gly Leu 
2945                2950                2955                2960
Asp Leu Leu Val Glu Phe Glu Phe Arg Thr Thr Thr Thr Thr Gly Val 
                2965                2970                2975    
Leu Leu Gly Ile Ser Ser Gln Lys Met Asp Gly Met Gly Ile Glu Met 
            2980                2985                2990        
Ile Asp Glu Lys Leu Met Phe His Val Asp Asn Gly Ala Gly Arg Phe 
        2995                3000                3005            
Thr Ala Val Tyr Asp Ala Gly Val Pro Gly His Leu Cys Asp Gly Gln 
    3010                3015                3020                
Trp His Lys Val Thr Ala Asn Lys Ile Lys His Arg Ile Glu Leu Thr 
3025                3030                3035                3040
Val Asp Gly Asn Gln Val Glu Ala Gln Ser Pro Asn Pro Ala Ser Thr 
                3045                3050                3055    
Ser Ala Asp Thr Asn Asp Pro Val Phe Val Gly Gly Phe Pro Asp Asp 
            3060                3065                3070        
Leu Lys Gln Phe Gly Leu Thr Thr Ser Ile Pro Phe Arg Gly Cys Ile 
        3075                3080                3085            
Arg Ser Leu Lys Leu Thr Lys Gly Thr Gly Lys Pro Leu Glu Val Asn 
    3090                3095                3100                
Phe Ala Lys Ala Leu Glu Leu Arg Gly Val Gln Pro Val Ser Cys Pro 
3105                3110                3115                3120
Ala Asn Ser 
            

<210> 75
<211> 9369
<212> DNA
<213> homo sapiens


<400> 75
atgccgggag ccgccggggt cctcctcctt ctgctgctct ccggaggcct cgggggcgta       60

caggcgcaga ggccgcagca gcagcggcag tcacaggcac atcagcaaag aggtttattc      120

cctgctgtcc tgaatcttgc ttctaatgct cttatcacga ccaatgcaac atgtggagaa      180

aaaggacctg aaatgtactg caaattggta gaacatgtcc ctgggcagcc tgtgaggaac      240

ccgcagtgtc gaatctgcaa tcaaaacagc agcaatccaa accagagaca cccgattaca      300

aatgctattg atggaaagaa cacttggtgg cagagtccca gtattaagaa tggaatcgaa      360

taccattatg tgacaattac cctggattta cagcaggtgt tccagatcgc gtatgtgatt      420

gtgaaggcag ctaactcccc ccggcctgga aactggattt tggaacgctc tcttgatgat      480

gttgaataca agccctggca gtatcatgct gtgacagaca cggagtgcct aacgctttac      540

aatatttatc cccgcactgg gccaccgtca tatgccaaag atgatgaggt catctgcact      600

tcattttact ccaagataca ccccttagaa aatggagaga ttcacatctc tttaatcaat      660

gggagaccaa gtgccgatga tccttctcca gaactgctag aatttacctc cgctcgctat      720

attcgcctga gatttcagag gatccgcaca ctgaatgctg acttgatgat gtttgctcac      780

aaagacccaa gagaaattga ccccattgtc accagaagat attactactc ggtcaaggat      840

atttcagttg gagggatgtg catctgctat ggtcatgcca gggcttgtcc acttgatcca      900

gcgacaaata aatctcgctg tgagtgtgag cataacacat gtggcgatag ctgtgatcag      960

tgctgtccag gattccatca gaaaccctgg agagctggaa cttttctaac taaaactgaa     1020

tgtgaagcat gcaattgtca tggaaaagct gaagaatgct attatgatga aaatgttgcc     1080

agaagaaatc tgagtttgaa tatacgtgga aagtacattg gagggggtgt ctgcattaat     1140

tgtacccaaa acactgctgg tataaactgc gagacatgta ctgatggctt cttcagaccc     1200

aaaggggtat ctccaaatta tccaaggcca tgccagccat gtcattgcga tccaattggt     1260

tccttaaatg aagtctgtgt caaggatgag aaacatgctc gacgaggttt ggcacctgga     1320

tcctgtcatt gcaaaactgg ttttggaggt gtgagctgtg atcggtgtgc caggggctac     1380

actggctacc cggactgcaa agcctgtaac tgcagtgggt tagggagcaa aaatgaggat     1440

ccttgttttg gcccctgtat ctgcaaggaa aatgttgaag gaggagactg tagtcgttgc     1500

aaatccggct tcttcaattt gcaagaggat aattggaaag gctgcgatga gtgtttctgt     1560

tcaggggttt caaacagatg tcagagttcc tactggacct atggcaaaat acaagatatg     1620

agtggctggt atctgactga ccttcctggc cgcattcgag tggctcccca gcaggacgac     1680

ttggactcac ctcagcagat cagcatcagt aacgcggagg cccggcaagc cctgccgcac     1740

agctactact ggagcgcgcc ggctccctat ctgggaaaca aactcccagc agtaggagga     1800

cagttgacat ttaccatatc atatgacctt gaagaagagg aagaagatac agaacgtgtt     1860

ctccagctta tgattatctt agagggtaat gacttgagca tcagcacagc ccaagatgag     1920

gtgtacctgc acccatctga agaacatact aatgtattgt tacttaaaga agaatcattt     1980

accatacatg gcacacattt tccagtccgt agaaaggaat ttatgacagt gcttgcgaat     2040

ttgaagagag tcctcctaca aatcacatac agctttggga tggatgccat cttcaggttg     2100

agctctgtta accttgaatc cgctgtctcc tatcctactg atggaagcat tgcagcagct     2160

gtagaagtgt gtcagtgccc accagggtat actggctcct cttgtgaatc ttgttggcct     2220

aggcacaggc gagttaacgg cactattttt ggtggcatct gtgagccatg tcagtgcttt     2280

ggtcatgcgg agtcctgtga tgacgtcact ggagaatgcc tgaactgtaa ggatcacaca     2340

ggtggcccat attgtgataa atgtcttcct ggtttctatg gcgagcctac taaaggaacc     2400

tctgaagact gtcaaccctg tgcctgtcca ctcaatatcc catccaataa ctttagccca     2460

acgtgccatt tagaccggag tcttggattg atctgtgatg gatgccctgt cgggtacaca     2520

ggaccacgct gtgagaggtg tgcagaaggc tattttggac aaccctctgt acctggagga     2580

tcatgtcagc catgccaatg caatgacaac cttgacttct ccatccctgg cagctgtgac     2640

agcttgtctg gctcctgtct gatatgtaaa ccaggtacaa caggccggta ctgtgagctc     2700

tgtgctgatg gatattttgg agatgcagtt gatgcgaaga actgtcagcc ctgtcgctgt     2760

aatgccggtg gctctttctc tgaggtttgc cacagtcaaa ctggacagtg tgagtgcaga     2820

gccaacgttc agggtcagag atgtgacaaa tgcaaggctg ggacctttgg cctacaatca     2880

gcaaggggct gtgttccctg caactgcaat tcttttgggt ctaagtcatt cgactgtgaa     2940

gagagtggac aatgttggtg ccaacctgga gtcacaggga agaaatgtga ccgctgtgcc     3000

cacggctatt tcaacttcca agaaggaggc tgcacagctt gtgaatgttc tcatctgggt     3060

aataattgtg acccaaagac tgggcgatgc atttgccctc ccaataccat tggagagaaa     3120

tgttctaaat gtgcacccaa tacctggggc cacagcatta ccactggttg taaggcttgt     3180

aactgcagca cagtgggatc cttggatttc caatgcaatg taaatacagg ccaatgcaac     3240

tgtcatccaa aattctctgg tgcaaaatgt acagagtgca gtcgaggtca ctggaactac     3300

cctcgctgca atctctgtga ctgcttcctc cctgggacag atgccacaac ctgtgattca     3360

gagactaaaa aatgctcctg tagtgatcaa actgggcagt gcacttgtaa ggtgaatgtg     3420

gaaggcatcc actgtgacag atgccggcct ggcaaattcg gactcgatgc caagaatcca     3480

cttggctgca gcagctgcta ttgcttcggc actactaccc agtgctctga agcaaaagga     3540

ctgatccgga cgtgggtgac tctgaaggct gagcagacca ttctacccct ggtagatgag     3600

gctctgcagc acacgaccac caagggcatt gtttttcaac atccagagat tgttgcccac     3660

atggacctga tgagagaaga tctccatttg gaaccttttt attggaaact tccagaacaa     3720

tttgaaggaa agaagttgat ggcctatggg ggcaaactca agtatgcaat ctatttcgag     3780

gctcgggaag aaacaggttt ctctacatat aatcctcaag tgatcattcg aggtgggaca     3840

cctactcatg ctagaattat cgtcaggcat atggctgctc ctctgattgg ccaattgaca     3900

aggcatgaaa ttgaaatgac agagaaagaa tggaaatatt atggggatga tcctcgagtc     3960

catagaactg tgacccgaga agacttcttg gatatactat atgatattca ttacattctt     4020

atcaaagcta cttatggaaa tttcatgcga caaagcagga tttctgaaat ctcaatggag     4080

gtagctgaac aaggacgtgg aacaacaatg actcctccag ctgacttgat tgaaaaatgt     4140

gattgtcccc tgggctattc tggcctgtcc tgtgaggcat gcttgccggg attttatcga     4200

ctgcgttctc aaccaggtgg ccgcacccct ggaccaaccc tgggcacctg tgttccatgt     4260

caatgtaatg gacacagcag cctgtgtgac cctgaaacat cgatatgcca gaattgtcaa     4320

catcacactg ctggtgactt ctgtgaacga tgtgctcttg gatactatgg aattgtcaag     4380

ggattgccaa atgactgtca gcaatgtgcc tgccctctga tttcttccag taacaatttc     4440

agcccctctt gtgtcgcaga aggacttgac gactaccgct gcacggcttg tccacgggga     4500

tatgaaggcc agtactgtga aaggtgtgcc cctggctata ctggcagtcc aggcaaccct     4560

ggaggctcct gccaagaatg tgagtgtgat ccctatggct cactgcctgt gccctgtgac     4620

cctgtcacag gattctgcac gtgccgacct ggagccacgg gaaggaagtg tgacggctgc     4680

aagcactggc atgcacgcga gggctgggag tgtgtttttt gtggagatga gtgcactggc     4740

cttcttctcg gtgacttggc tcgcctggag cagatggtca tgagcatcaa cctcactggt     4800

ccgctgcctg cgccatataa aatgctgtat ggtcttgaaa atatgactca ggagctaaag     4860

cacttgctgt cacctcagcg ggccccagag aggcttattc agctggcaga gggcaatctg     4920

aatacactcg tgaccgaaat gaacgagctg ctgaccaggg ctaccaaagt gacagcagat     4980

ggcgagcaga ccggacagga tgctgagagg accaacacaa gagcaaagtc cctgggagaa     5040

ttcattaagg agcttgcccg ggatgcagaa gctgtaaatg aaaaagctat aaaactaaat     5100

gaaactctag gaactcgaga tgaggccttt gagagaaatt tggaagggct tcagaaagag     5160

attgaccaga tgattaaaga actgaggagg aaaaatctag agacacaaaa ggaaattgct     5220

gaagatgagt tggtagctgc agaagccctt ctgaaaaaag tgaagaagct gtttggagag     5280

tcccgggggg aaaatgaaga aatggagaag gatctccggg aaaaactggc tgactacaaa     5340

aacaaagttg atgatgcttg ggaccttttg agagaagcca cagataaaat cagagaagct     5400

aatcgcctat ttgcagtaaa tcagaaaaac atgactgcat tggagaaaaa gaaggaggct     5460

gttgaaagcg gcaaacgaca aattgagaac actttaaaag agggcaatga catactcgat     5520

gaagccaacc gtcttgcaga tgaaatcaac tccatcatag actatgttga agacatccaa     5580

actaaattgc cacctatgtc tgaggagctt aatgataaaa tagatgacct ctcccaagaa     5640

ataaaggaca ggaaacttgc tgagaaggtg tcccaggctg agagccacgc agctcagttg     5700

aatgactcat ctgctgtcct tgatggaatc cttgatgagg ctaaaaacat ctccttcaat     5760

gccactgcag ccttcaaagc ctacagcaat attaaggact atattgatga agctgagaaa     5820

gttgccaaag aagccaaaga tcttgcacat gaagctacaa aactggcaac aggtcctcgg     5880

ggtttattaa aggaagatgc caaaggctgt cttcagaaaa gtttcaggat tcttaacgaa     5940

gccaagaagt tagcaaatga tgtaaaagaa aatgaagacc atctaaatgg cttaaaaacc     6000

aggatagaaa atgctgatgc tagaaatggg gatctcttga gaactttgaa tgacactttg     6060

ggaaagttat cagctattcc aaatgataca gctgctaaac tgcaagctgt taaggacaaa     6120

gccagacaag ccaacgacac agctaaagat gtactggcac agattacaga gctccaccag     6180

aacctcgatg gcctgaagaa gaattacaat aaactagcag acagcgtcgc caaaacgaat     6240

gctgtggtta aagatccttc caagaacaaa atcattgccg atgcagatgc cactgtcaaa     6300

aatttagaac aggaagctga ccggctaata gataaactca aacccatcaa ggaacttgag     6360

gataacctaa agaaaaacat ctctgagata aaggaattga taaaccaagc tcggaaacaa     6420

gccaattcta tcaaagtatc tgtgtcttca ggaggtgact gcattcgaac atacaaacca     6480

gaaatcaaga aaggaagtta caataatatt gttgtcaacg taaagacagc tgttgctgat     6540

aacctcctct tttatcttgg aagtgccaaa tttattgact ttctggctat agaaatgcgt     6600

aaaggcaaag tcagcttcct ctgggatgtt ggatctggag ttggacgtgt agagtaccca     6660

gatttgacta ttgatgactc atattggtac cgtatcgtag catcaagaac tgggagaaat     6720

ggaactattt ctgtgagagc cctggatgga cccaaagcca gcattgtgcc cagcacacac     6780

cattcgacgt cccctccagg gtacacgatt ctagatgtgg atgcaaatgc aatgctgttt     6840

gttggtggcc tgactgggaa attaaagaag gctgatgctg tacgtgtgat tacattcact     6900

ggctgcatgg gagaaacata ctttgacaac aaacctatag gtttgtggaa tttccgagaa     6960

aaagaaggtg actgcaaagg atgcactgtc agtcctcagg tggaagatag tgaggggact     7020

attcaatttg atggagaagg ttatgcattg gtcagccgtc ccattcgctg gtaccccaac     7080

atctccactg tcatgttcaa gttcagaaca ttttcttcga gtgcccttct gatgtatctt     7140

gccacacgag acctgagaga tttcatgagt gtggagctca ctgatgggca cataaaagtc     7200

agttacgatc tgggctcagg aatggcttcc gttgtcagca atcaaaacca taatgatggg     7260

aaatggaaat cattcactct gtcaagaatt caaaaacaag ccaatatatc aattgtagat     7320

atagatacta atcaggagga gaatatagca acttcgtctt ctggaaacaa ctttggtctt     7380

gacttgaaag cagatgacaa aatatatttt ggtggcctgc caacgctgag aaacttgagt     7440

atgaaagcaa ggccagaagt aaatctgaag aaatattccg gctgcctcaa agatattgaa     7500

atttcaagaa ctccgtacaa tatactcagt agtcccgatt atgttggtgt taccaaagga     7560

tgttccctgg agaatgttta cacagttagc tttcctaagc ctggttttgt ggagctctcc     7620

cctgtgccaa ttgatgtagg aacagaaatc aacctgtcat tcagcaccaa gaatgagtcc     7680

ggcatcattc ttttgggaag tggagggaca ccagcaccac ctaggagaaa acgaaggcag     7740

actggacagg cctattatgt aatactcctc aacaggggcc gtctggaagt gcatctctcc     7800

acaggggcac gaacaatgag gaaaattgtg atcagaccag agccgaatct gtttcatgat     7860

ggaagagaac attccgttca tgtagagcga actagaggca tctttacagt tcaagtggat     7920

gaaaacagaa gatacatgca aaacctgaca gttgaacagc ctatcgaagt taaaaaactt     7980

ttcgttgggg gtgctccacc tgaatttcaa ccttccccac tcagaaatat tcctcctttt     8040

gaaggctgca tatggaatct tgttattaac tctgtcccca tggactttgc aaggcctgtg     8100

tccttcaaaa atgctgacat tggtcgctgt gcccatcaga aactccgtga agatgaagat     8160

ggagcagctc cagctgaaat agttatccag cctgagccag ttcccacccc agcctttcct     8220

acgcccaccc cagttctgac acatggtcct tgtgctgcag aatcagaacc agctcttttg     8280

atagggagca agcagttcgg gctttcaaga aacagtcaca ttgcaattgc atttgatgac     8340

accaaagtta aaaaccgcct cacaattgag ttggaagtaa gaaccgaagc tgaatccggc     8400

ttgctttttt acatggctcg catcaatcat gctgattttg caacagttca gctgagaaat     8460

ggattgccct acttcagcta tgacttgggg agtggggaca cccacaccat gatccccacc     8520

aaaatcaatg atggccagtg gcacaagatt aagataatga gaagtaagca agaaggaatt     8580

ctttatgtag atggggcttc caacagaacc atcagtccca aaaaagccga catcctggat     8640

gtcgtgggaa tgctgtatgt tggtgggtta cccatcaact acactacccg aagaattggt     8700

ccagtgacct atagcattga tggctgcgtc aggaatctcc acatggcaga ggcccctgcc     8760

gatctggaac aacccacctc cagcttccat gttgggacat gttttgcaaa tgctcagagg     8820

ggaacatatt ttgacggaac cggttttgcc aaagcagttg gtggattcaa agtgggattg     8880

gaccttcttg tagaatttga attccgcaca actacaacga ctggagttct tctggggatc     8940

agtagtcaaa aaatggatgg aatgggtatt gaaatgattg atgaaaagtt gatgtttcat     9000

gtggacaatg gtgcgggcag attcactgct gtctatgatg ctggggttcc agggcatttg     9060

tgtgatggac aatggcataa agtcactgcc aacaagatca aacaccgcat tgagctcaca     9120

gtcgatggga accaggtgga agcccaaagc ccaaacccag catctacatc agctgacaca     9180

aatgaccctg tgtttgttgg aggcttccca gatgacctca agcagtttgg cctaacaacc     9240

agtattccgt tccgaggttg catcagatcc ctgaagctca ccaaaggcac aggcaagcca     9300

ctggaggtta attttgccaa ggccctggaa ctgaggggcg ttcaacctgt atcatgccca     9360

gccaactca                                                             9369


<210> 76
<211> 508
<212> DNA
<213> Artificial sequence


<220> 
<223> CMV promoter

<400> 76
cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      60

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca     120

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc     180

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta     240

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac     300

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg     360

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg     420

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt     480

acggtgggag gtctatataa gcagagct                                        508


<210> 77
<211> 1651
<212> DNA
<213> Artificial sequence


<220> 
<223> CAG promoter

<400> 77
acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg      60

tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg     120

gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt     180

acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg     240

accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg     300

gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca     360

attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg     420

gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt     480

gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg     540

cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg ggagtcgctg cgacgctgcc     600

ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg     660

cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct     720

tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg     780

agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga     840

gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc     900

tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg     960

gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag    1020

ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg    1080

agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc    1140

cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg    1200

agggctcggg ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga    1260

gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc    1320

aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg    1380

cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc    1440

gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc    1500

gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct    1560

ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta    1620

ttgtgctgtc tcatcatttt ggcaaagaat t                                   1651


<210> 78
<211> 546
<212> DNA
<213> Artificial sequence


<220> 
<223> EF1-alpha Promoter

<400> 78
aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc      60

cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt     120

aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc     180

gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac     240

acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg     300

ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac     360

tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct     420

cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca     480

actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc     540

gcctac                                                                546


<210> 79
<211> 317
<212> DNA
<213> Artificial sequence


<220> 
<223> SV-40 Promoter

<400> 79
ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt      60

agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca     120

tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa     180

ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag     240

aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag     300

gcctaggctt ttgcaaa                                                    317


<210> 80
<211> 725
<212> DNA
<213> Artificial sequence


<220> 
<223> EalbAATp

<400> 80
gacgcgcatg ctcctctaga ctcgacgcgt gttcctagat tacactacac attctgcaag      60

catagcacag agcaatgttc tactttaatt actttcattt tcttgtatcc tcacagccta     120

gaaaataacc tgcgttacag catccactca gtatcccttg agcatgaggt gacactactt     180

aacataggga cgagatggta ctttgtgtct cctgctctgt cagcagggca cagtacttgc     240

tgataccagg gaatgtttgt tcttaaatac catcattccg gacgtgtttg ccttggccag     300

ttttccatgt acatgcagaa agaagtttgg actgatcaat acagtcctct gcctttaaag     360

caataggaaa aggccaactt gtctacgttt agtatgtggc tgtagatctg tacccgccac     420

cccctccacc ttggacacag gacgctgtgg tttctgagcc aggtacaatg actcctttcg     480

gtaagtgcag tggaagctgt acactgccca ggcaaagcgt ccgggcagcg taggcgggcg     540

actcagatcc cagccagtgg acttagcccc tgtttgctcc tccgataact ggggtgacct     600

tggttaatat tcaccagcag cctcccccgt tgcccctctg gatccactgc ttaaatacgg     660

acgaggacag ggccctgtct cctcagcttc aggcaccacc actgacctgg gacagtgaag     720

cggcc                                                                 725


<210> 81
<211> 51
<212> DNA
<213> Artificial sequence


<220> 
<223> Splice Acceptor

<400> 81
gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca g              51


<210> 82
<211> 86
<212> DNA
<213> artificial sequence


<220> 
<223> synthetic ama2 intron 26

<400> 82
gtaagttagt cattgtttgg tgcaaagata ccaatcaatg gttttgcatt cagttttgtc     60

atagtgattt ctcttcttgt tttcag                                          86


<210> 83
<211> 131
<212> DNA
<213> artificial sequence


<220> 
<223> SV40-PolyA

<400> 83
aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca     60

aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct    120

tatcatgtct g                                                         131


<210> 84
<211> 263
<212> DNA
<213> artificial sequence


<220> 
<223> BHG-PolyA

<400> 84
gtgtcaccta aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc     60

atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt    120

cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct    180

ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc    240

tggggatgcg gtgggctcta tgg                                            263


<210> 85
<211> 49
<212> DNA
<213> artificial sequence


<220> 
<223> HSV-Tk-PolyA

<400> 85
cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttc                 49


<210> 86
<211> 232
<212> DNA
<213> artificial sequence


<220> 
<223> Insulator a

<400> 86
gagggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc ccgctagggg     60

gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca tccccgagcc    120

ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct ttcctctgaa    180

cgcttctcgc tgctctttga gcctgcagac acctgggggg atacggggaa aa            232


<210> 87
<211> 231
<212> DNA
<213> artificial sequence


<220> 
<223> insulator b

<400> 87
ttttccccgt atccccccag gtgtctgcag gctcaaagag cagcgagaag cgttcagagg     60

aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg ccggctcggg    120

gatgcggggg gagcgccgga ccggagcgga gccccgggcg gctcgctgct gccccctagc    180

gggggaggga cgtaattaca tccctggggg ctttgggggg gggctgtccc t             231


<210> 88
<211> 63
<212> DNA
<213> artificial sequence


<220> 
<223> ITR-5'

<400> 88
ccctagaaag ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc     60

atg                                                                   63


<210> 89
<211> 35
<212> DNA
<213> artificial sequence


<220> 
<223> ITR-3'

<400> 89
catgcgtcaa ttttacgcag actatctttc taggg                                35


<210> 90
<211> 20
<212> DNA
<213> artificial sequence


<220> 
<223> gRNA-Rosa26-1

<400> 90
agtctttcta gaagatgggc                                                20


<210> 91
<211> 20
<212> DNA
<213> artificial sequence


<220> 
<223> gRNA-Rosa26-2

<400> 91
cagtctttct agaagatggg                                                20


<210> 92
<211> 20
<212> DNA
<213> artificial sequence


<220> 
<223> gRNA-Albumin-1

<400> 92
caatctttaa atatgttgtg                                                20


<210> 93
<211> 20
<212> DNA
<213> artificial sequence


<220> 
<223> gRNA-Rosa26-4

<400> 93
actccagtct ttctagaaga                                                20


<210> 94
<211> 20
<212> DNA
<213> artificial sequence


<220> 
<223> gRNA-Albumin-1

<400> 94
caatctttaa atatgttgtg                                                20


<210> 95
<211> 20
<212> DNA
<213> artificial sequence


<220> 
<223> gRNA-Albumin-2

<400> 95
gttacaggaa aatctgaagg                                                20


<210> 96
<211> 20
<212> DNA
<213> artificial sequence


<220> 
<223> gRNA-Albumin-3

<400> 96
taactttgag tgtagcagag                                                20


<210> 97
<211> 20
<212> DNA
<213> artificial sequence


<220> 
<223> gRNA-Albumin-4

<400> 97
tatagcatgg tcgagcaggc                                                20


<210> 98
<211> 3117
<212> PRT
<213> mus musculus


<400> 98
Met Pro Ala Ala Thr Ala Gly Ile Leu Leu Leu Leu Leu Leu Gly Thr 
1               5                   10                  15      
Leu Glu Gly Ser Gln Thr Gln Arg Arg Gln Ser Gln Ala His Gln Gln 
            20                  25                  30          
Arg Gly Leu Phe Pro Ala Val Leu Asn Leu Ala Ser Asn Ala Leu Ile 
        35                  40                  45              
Thr Thr Asn Ala Thr Cys Gly Glu Lys Gly Pro Glu Met Tyr Cys Lys 
    50                  55                  60                  
Leu Val Glu His Val Pro Gly Gln Pro Val Arg Asn Pro Gln Cys Arg 
65                  70                  75                  80  
Ile Cys Asn Gln Asn Ser Ser Asn Pro Tyr Gln Arg His Pro Ile Thr 
                85                  90                  95      
Asn Ala Ile Asp Gly Lys Asn Thr Trp Trp Gln Ser Pro Ser Ile Lys 
            100                 105                 110         
Asn Gly Val Glu Tyr His Tyr Val Thr Ile Thr Leu Asp Leu Gln Gln 
        115                 120                 125             
Val Phe Gln Ile Ala Tyr Val Ile Val Lys Ala Ala Asn Ser Pro Arg 
    130                 135                 140                 
Pro Gly Asn Trp Ile Leu Glu Arg Ser Leu Asp Asp Val Glu Tyr Lys 
145                 150                 155                 160 
Pro Trp Gln Tyr His Ala Val Thr Asp Thr Glu Cys Leu Thr Leu Tyr 
                165                 170                 175     
Asn Ile Tyr Pro Arg Thr Gly Pro Pro Ser Tyr Ala Lys Asp Asp Glu 
            180                 185                 190         
Val Ile Cys Thr Ser Phe Tyr Ser Lys Ile His Pro Leu Glu Asn Gly 
        195                 200                 205             
Glu Ile His Ile Ser Leu Ile Asn Gly Arg Pro Ser Ala Asp Asp Pro 
    210                 215                 220                 
Ser Pro Glu Leu Leu Glu Phe Thr Ser Ala Arg Tyr Ile Arg Leu Arg 
225                 230                 235                 240 
Phe Gln Arg Ile Arg Thr Leu Asn Ala Asp Leu Met Met Phe Ala His 
                245                 250                 255     
Lys Asp Pro Arg Glu Ile Asp Pro Ile Val Thr Arg Arg Tyr Tyr Tyr 
            260                 265                 270         
Ser Val Lys Asp Ile Ser Val Gly Gly Met Cys Ile Cys Tyr Gly His 
        275                 280                 285             
Ala Arg Ala Cys Pro Leu Asp Pro Ala Thr Asn Lys Ser Arg Cys Glu 
    290                 295                 300                 
Cys Glu His Asn Thr Cys Gly Glu Ser Cys Asp Arg Cys Cys Pro Gly 
305                 310                 315                 320 
Phe His Gln Lys Pro Trp Arg Ala Gly Thr Phe Leu Thr Lys Ser Glu 
                325                 330                 335     
Cys Glu Ala Cys Asn Cys His Gly Lys Ala Glu Glu Cys Tyr Tyr Asp 
            340                 345                 350         
Glu Thr Val Ala Ser Arg Asn Leu Ser Leu Asn Ile His Gly Lys Tyr 
        355                 360                 365             
Ile Gly Gly Gly Val Cys Ile Asn Cys Thr His Asn Thr Ala Gly Ile 
    370                 375                 380                 
Asn Cys Glu Thr Cys Val Asp Gly Phe Phe Arg Pro Lys Gly Val Ser 
385                 390                 395                 400 
Pro Asn Tyr Pro Arg Pro Cys Gln Pro Cys His Cys Asp Pro Thr Gly 
                405                 410                 415     
Ser Leu Ser Glu Val Cys Val Lys Asp Glu Lys Tyr Ala Gln Arg Gly 
            420                 425                 430         
Leu Lys Pro Gly Ser Cys His Cys Lys Thr Gly Phe Gly Gly Val Asn 
        435                 440                 445             
Cys Asp Arg Cys Val Arg Gly Tyr His Gly Tyr Pro Asp Cys Gln Pro 
    450                 455                 460                 
Cys Asn Cys Ser Gly Leu Gly Ser Thr Asn Glu Asp Pro Cys Val Gly 
465                 470                 475                 480 
Pro Cys Ser Cys Lys Glu Asn Val Glu Gly Glu Asp Cys Ser Arg Cys 
                485                 490                 495     
Lys Ser Gly Phe Phe Asn Leu Gln Glu Asp Asn Gln Lys Gly Cys Glu 
            500                 505                 510         
Glu Cys Phe Cys Ser Gly Val Ser Asn Arg Cys Gln Ser Ser Tyr Trp 
        515                 520                 525             
Thr Tyr Gly Asn Ile Gln Asp Met Arg Gly Trp Tyr Leu Thr Asp Leu 
    530                 535                 540                 
Ser Gly Arg Ile Arg Met Ala Pro Gln Leu Asp Asn Pro Asp Ser Pro 
545                 550                 555                 560 
Gln Gln Ile Ser Ile Ser Asn Ser Glu Ala Arg Lys Ser Leu Leu Asp 
                565                 570                 575     
Gly Tyr Tyr Trp Ser Ala Pro Pro Pro Tyr Leu Gly Asn Arg Leu Pro 
            580                 585                 590         
Ala Val Gly Gly Gln Leu Ser Phe Thr Ile Ser Tyr Asp Leu Glu Glu 
        595                 600                 605             
Glu Glu Asp Asp Thr Glu Lys Ile Leu Gln Leu Met Ile Ile Phe Glu 
    610                 615                 620                 
Gly Asn Asp Leu Arg Ile Ser Thr Ala Tyr Lys Glu Val Tyr Leu Glu 
625                 630                 635                 640 
Pro Ser Glu Glu His Ile Glu Glu Val Ser Leu Lys Glu Glu Ala Phe 
                645                 650                 655     
Thr Ile His Gly Thr Asn Leu Pro Val Thr Arg Lys Asp Phe Met Ile 
            660                 665                 670         
Val Leu Thr Asn Leu Glu Arg Val Leu Met Gln Ile Thr Tyr Asn Leu 
        675                 680                 685             
Gly Met Asp Ala Ile Phe Arg Leu Ser Ser Val Asn Leu Glu Ser Ala 
    690                 695                 700                 
Val Pro Tyr Pro Thr Asp Arg Arg Ile Ala Thr Asp Val Glu Val Cys 
705                 710                 715                 720 
Gln Cys Pro Pro Gly Tyr Ser Gly Ser Ser Cys Glu Thr Cys Trp Pro 
                725                 730                 735     
Arg His Arg Arg Val Asn Gly Thr Ile Phe Gly Gly Ile Cys Glu Pro 
            740                 745                 750         
Cys Gln Cys Phe Ala His Ala Glu Ala Cys Asp Asp Ile Thr Gly Glu 
        755                 760                 765             
Cys Leu Asn Cys Lys Asp His Thr Gly Gly Pro Tyr Cys Asn Glu Cys 
    770                 775                 780                 
Leu Pro Gly Phe Tyr Gly Asp Pro Thr Arg Gly Ser Pro Glu Asp Cys 
785                 790                 795                 800 
Gln Pro Cys Ala Cys Pro Leu Asn Ile Pro Ser Asn Asn Phe Ser Pro 
                805                 810                 815     
Thr Cys His Leu Asp Arg Ser Leu Gly Leu Ile Cys Asp Glu Cys Pro 
            820                 825                 830         
Ile Gly Tyr Thr Gly Pro Arg Cys Glu Arg Cys Ala Glu Gly Tyr Phe 
        835                 840                 845             
Gly Gln Pro Ser Ile Pro Gly Gly Ser Cys Gln Pro Cys Gln Cys Asn 
    850                 855                 860                 
Asp Asn Leu Asp Tyr Ser Ile Pro Gly Ser Cys Asp Ser Leu Ser Gly 
865                 870                 875                 880 
Ser Cys Leu Ile Cys Lys Pro Gly Thr Thr Gly Arg Tyr Cys Glu Leu 
                885                 890                 895     
Cys Ala Asp Gly Tyr Phe Gly Asp Ala Val Asn Ala Lys Asn Cys Gln 
            900                 905                 910         
Pro Cys Arg Cys Asn Ile Asn Gly Ser Phe Ser Glu Ile Cys His Thr 
        915                 920                 925             
Arg Thr Gly Gln Cys Glu Cys Arg Pro Asn Val Gln Gly Arg His Cys 
    930                 935                 940                 
Asp Glu Cys Lys Pro Glu Thr Phe Gly Leu Gln Leu Gly Arg Gly Cys 
945                 950                 955                 960 
Leu Pro Cys Asn Cys Asn Ser Phe Gly Ser Lys Ser Phe Asp Cys Glu 
                965                 970                 975     
Ala Ser Gly Gln Cys Trp Cys Gln Pro Gly Val Ala Gly Lys Lys Cys 
            980                 985                 990         
Asp Arg Cys Ala His Gly Tyr Phe Asn Phe Gln Glu Gly Gly Cys Ile 
        995                 1000                1005            
Ala Cys Asp Cys Ser His Leu Gly Asn Asn Cys Asp Pro Lys Thr Gly 
    1010                1015                1020                
Gln Cys Ile Cys Pro Pro Asn Thr Thr Gly Glu Lys Cys Ser Glu Cys 
1025                1030                1035                1040
Leu Pro Asn Thr Trp Gly His Ser Ile Val Thr Gly Cys Lys Val Cys 
                1045                1050                1055    
Asn Cys Ser Thr Val Gly Ser Leu Ala Ser Gln Cys Asn Val Asn Thr 
            1060                1065                1070        
Gly Gln Cys Ser Cys His Pro Lys Phe Ser Gly Met Lys Cys Ser Glu 
        1075                1080                1085            
Cys Ser Arg Gly His Trp Asn Tyr Pro Leu Cys Thr Leu Cys Asp Cys 
    1090                1095                1100                
Phe Leu Pro Gly Thr Asp Ala Thr Thr Cys Asp Leu Glu Thr Arg Lys 
1105                1110                1115                1120
Cys Ser Cys Ser Asp Gln Thr Gly Gln Cys Ser Cys Lys Val Asn Val 
                1125                1130                1135    
Glu Gly Val His Cys Asp Arg Cys Arg Pro Gly Lys Phe Gly Leu Asp 
            1140                1145                1150        
Ala Lys Asn Pro Leu Gly Cys Ser Ser Cys Tyr Cys Phe Gly Val Thr 
        1155                1160                1165            
Ser Gln Cys Ser Glu Ala Lys Gly Leu Ile Arg Thr Trp Val Thr Leu 
    1170                1175                1180                
Ser Asp Glu Gln Thr Ile Leu Pro Leu Val Asp Glu Ala Leu Gln His 
1185                1190                1195                1200
Thr Thr Thr Lys Gly Ile Ala Phe Gln Lys Pro Glu Ile Val Ala Lys 
                1205                1210                1215    
Met Asp Glu Val Arg Gln Glu Leu His Leu Glu Pro Phe Tyr Trp Lys 
            1220                1225                1230        
Leu Pro Gln Gln Phe Glu Gly Lys Lys Leu Met Ala Tyr Gly Gly Lys 
        1235                1240                1245            
Leu Lys Tyr Ala Ile Tyr Phe Glu Ala Arg Asp Glu Thr Gly Phe Ala 
    1250                1255                1260                
Thr Tyr Lys Pro Gln Val Ile Ile Arg Gly Gly Thr Pro Thr His Ala 
1265                1270                1275                1280
Arg Ile Ile Thr Arg His Met Ala Ala Pro Leu Ile Gly Gln Leu Thr 
                1285                1290                1295    
Arg His Glu Ile Glu Met Thr Glu Lys Glu Trp Lys Tyr Tyr Gly Asp 
            1300                1305                1310        
Asp Pro Arg Ile Ser Arg Thr Val Thr Arg Glu Asp Phe Leu Asp Ile 
        1315                1320                1325            
Leu Tyr Asp Ile His Tyr Ile Leu Ile Lys Ala Thr Tyr Gly Asn Val 
    1330                1335                1340                
Val Arg Gln Ser Arg Ile Ser Glu Ile Ser Met Glu Val Ala Glu Pro 
1345                1350                1355                1360
Gly His Val Leu Ala Gly Ser Pro Pro Ala His Leu Ile Glu Arg Cys 
                1365                1370                1375    
Asp Cys Pro Pro Gly Tyr Ser Gly Leu Ser Cys Glu Thr Cys Ala Pro 
            1380                1385                1390        
Gly Phe Tyr Arg Leu Arg Ser Glu Pro Gly Gly Arg Thr Pro Gly Pro 
        1395                1400                1405            
Thr Leu Gly Thr Cys Val Pro Cys Gln Cys Asn Gly His Ser Ser Gln 
    1410                1415                1420                
Cys Asp Pro Glu Thr Ser Val Cys Gln Asn Cys Gln His His Thr Ala 
1425                1430                1435                1440
Gly Asp Phe Cys Glu Arg Cys Ala Leu Gly Tyr Tyr Gly Ile Val Arg 
                1445                1450                1455    
Gly Leu Pro Asn Asp Cys Gln Pro Cys Ala Cys Pro Leu Ile Ser Pro 
            1460                1465                1470        
Ser Asn Asn Phe Ser Pro Ser Cys Val Leu Glu Gly Leu Glu Asp Tyr 
        1475                1480                1485            
Arg Cys Thr Ala Cys Pro Arg Gly Tyr Glu Gly Gln Tyr Cys Glu Arg 
    1490                1495                1500                
Cys Ala Pro Gly Tyr Thr Gly Ser Pro Ser Ser Pro Gly Gly Ser Cys 
1505                1510                1515                1520
Gln Glu Cys Glu Cys Asp Pro Tyr Gly Ser Leu Pro Val Pro Cys Asp 
                1525                1530                1535    
Arg Val Thr Gly Leu Cys Thr Cys Arg Pro Gly Ala Thr Gly Arg Lys 
            1540                1545                1550        
Cys Asp Gly Cys Glu His Trp His Ala Arg Glu Gly Ala Glu Cys Val 
        1555                1560                1565            
Phe Cys Gly Asp Glu Cys Thr Gly Leu Leu Leu Gly Asp Leu Ala Arg 
    1570                1575                1580                
Leu Glu Gln Met Thr Met Asn Ile Asn Leu Thr Gly Pro Leu Pro Ala 
1585                1590                1595                1600
Pro Tyr Lys Ile Leu Tyr Gly Leu Glu Asn Thr Thr Gln Glu Leu Lys 
                1605                1610                1615    
His Leu Leu Ser Pro Gln Arg Ala Pro Glu Arg Leu Ile Gln Leu Ala 
            1620                1625                1630        
Glu Gly Asn Val Asn Thr Leu Val Met Glu Thr Asn Glu Leu Leu Thr 
        1635                1640                1645            
Arg Ala Thr Lys Val Thr Ala Asp Gly Glu Gln Thr Gly Gln Asp Ala 
    1650                1655                1660                
Glu Arg Thr Asn Ser Arg Ala Glu Ser Leu Glu Glu Phe Ile Lys Gly 
1665                1670                1675                1680
Leu Val Gln Asp Ala Glu Ala Ile Asn Glu Lys Ala Val Gln Leu Asn 
                1685                1690                1695    
Glu Thr Leu Gly Asn Gln Asp Lys Thr Ala Glu Arg Asn Leu Glu Glu 
            1700                1705                1710        
Leu Gln Lys Glu Ile Asp Arg Met Leu Lys Glu Leu Arg Ser Lys Asp 
        1715                1720                1725            
Leu Gln Thr Gln Lys Glu Val Ala Glu Asp Glu Leu Val Ala Ala Glu 
    1730                1735                1740                
Gly Leu Leu Lys Arg Val Asn Lys Leu Phe Gly Glu Pro Arg Ala Gln 
1745                1750                1755                1760
Asn Glu Asp Met Glu Lys Asp Leu Gln Gln Lys Leu Ala Glu Tyr Lys 
                1765                1770                1775    
Asn Lys Leu Asp Asp Ala Trp Asp Leu Leu Arg Glu Ala Thr Asp Lys 
            1780                1785                1790        
Thr Arg Asp Ala Asn Arg Leu Ser Ala Ala Asn Gln Lys Asn Met Thr 
        1795                1800                1805            
Ile Leu Glu Thr Lys Lys Glu Ala Ile Glu Gly Ser Lys Arg Gln Ile 
    1810                1815                1820                
Glu Asn Thr Leu Lys Glu Gly Asn Asp Ile Leu Asp Glu Ala Asn Arg 
1825                1830                1835                1840
Leu Leu Gly Glu Ile Asn Ser Val Ile Asp Tyr Val Asp Asp Ile Lys 
                1845                1850                1855    
Thr Lys Leu Pro Pro Met Ser Glu Glu Leu Ser Asp Lys Ile Asp Asp 
            1860                1865                1870        
Leu Ala Gln Glu Ile Lys Asp Arg Arg Leu Ala Glu Lys Val Phe Gln 
        1875                1880                1885            
Ala Glu Ser His Ala Ala Gln Leu Asn Asp Ser Ser Ala Val Leu Asp 
    1890                1895                1900                
Gly Ile Leu Asp Glu Ala Lys Asn Ile Ser Phe Asn Ala Thr Ala Ala 
1905                1910                1915                1920
Phe Arg Ala Tyr Ser Asn Ile Lys Asp Tyr Ile Asp Glu Ala Glu Lys 
                1925                1930                1935    
Val Ala Arg Glu Ala Lys Glu Leu Ala Gln Gly Ala Thr Lys Leu Ala 
            1940                1945                1950        
Thr Ser Pro Gln Gly Leu Leu Lys Glu Asp Ala Lys Gly Ser Leu Gln 
        1955                1960                1965            
Lys Ser Phe Arg Ile Leu Asn Glu Ala Lys Lys Leu Ala Asn Asp Val 
    1970                1975                1980                
Lys Gly Asn His Asn Asp Leu Asn Asp Leu Lys Thr Arg Leu Glu Thr 
1985                1990                1995                2000
Ala Asp Leu Arg Asn Ser Gly Leu Leu Gly Ala Leu Asn Asp Thr Met 
                2005                2010                2015    
Asp Lys Leu Ser Ala Ile Thr Asn Asp Thr Ala Ala Lys Leu Gln Ala 
            2020                2025                2030        
Val Lys Glu Lys Ala Arg Glu Ala Asn Asp Thr Ala Lys Ala Val Leu 
        2035                2040                2045            
Ala Gln Val Lys Asp Leu His Gln Asn Leu Asp Gly Leu Lys Gln Asn 
    2050                2055                2060                
Tyr Asn Lys Leu Ala Asp Ser Val Ala Lys Thr Asn Ala Val Val Lys 
2065                2070                2075                2080
Asp Pro Ser Lys Asn Lys Ile Ile Ala Asp Ala Gly Thr Ser Val Arg 
                2085                2090                2095    
Asn Leu Glu Gln Glu Ala Asp Arg Leu Ile Asp Lys Leu Lys Pro Ile 
            2100                2105                2110        
Lys Glu Leu Glu Asp Asn Leu Lys Lys Asn Ile Ser Glu Ile Lys Glu 
        2115                2120                2125            
Leu Ile Asn Gln Ala Arg Lys Gln Ala Asn Ser Ile Lys Val Ser Val 
    2130                2135                2140                
Ser Ser Gly Gly Asp Cys Val Arg Thr Tyr Arg Pro Glu Ile Lys Lys 
2145                2150                2155                2160
Gly Ser Tyr Asn Asn Ile Val Val His Val Lys Thr Ala Val Ala Asp 
                2165                2170                2175    
Asn Leu Leu Phe Tyr Leu Gly Ser Ala Lys Phe Ile Asp Phe Leu Ala 
            2180                2185                2190        
Ile Glu Met Arg Lys Gly Lys Val Ser Phe Leu Trp Asp Val Gly Ser 
        2195                2200                2205            
Gly Val Gly Arg Val Glu Tyr Pro Asp Leu Thr Ile Asp Asp Ser Tyr 
    2210                2215                2220                
Trp Tyr Arg Ile Glu Ala Ser Arg Thr Gly Arg Asn Gly Ser Ile Ser 
2225                2230                2235                2240
Val Arg Ala Leu Asp Gly Pro Lys Ala Ser Met Val Pro Ser Thr Tyr 
                2245                2250                2255    
His Ser Val Ser Pro Pro Gly Tyr Thr Ile Leu Asp Val Asp Ala Asn 
            2260                2265                2270        
Ala Met Leu Phe Val Gly Gly Leu Thr Gly Lys Ile Lys Lys Ala Asp 
        2275                2280                2285            
Ala Val Arg Val Ile Thr Phe Thr Gly Cys Met Gly Glu Thr Tyr Phe 
    2290                2295                2300                
Asp Asn Lys Pro Ile Gly Leu Trp Asn Phe Arg Glu Lys Glu Gly Asp 
2305                2310                2315                2320
Cys Lys Gly Cys Thr Val Ser Pro Gln Val Glu Asp Ser Glu Gly Thr 
                2325                2330                2335    
Ile Gln Phe Asp Gly Glu Gly Tyr Ala Leu Val Ser Arg Pro Ile Arg 
            2340                2345                2350        
Trp Tyr Pro Asn Ile Ser Thr Val Met Phe Lys Phe Arg Thr Phe Ser 
        2355                2360                2365            
Ser Ser Ala Leu Leu Met Tyr Leu Ala Thr Arg Asp Leu Lys Asp Phe 
    2370                2375                2380                
Met Ser Val Glu Leu Ser Asp Gly His Val Lys Val Ser Tyr Asp Leu 
2385                2390                2395                2400
Gly Ser Gly Met Thr Ser Val Val Ser Asn Gln Asn His Asn Asp Gly 
                2405                2410                2415    
Lys Trp Lys Ala Phe Thr Leu Ser Arg Ile Gln Lys Gln Ala Asn Ile 
            2420                2425                2430        
Ser Ile Val Asp Ile Asp Ser Asn Gln Glu Glu Asn Val Ala Thr Ser 
        2435                2440                2445            
Ser Ser Gly Asn Asn Phe Gly Leu Asp Leu Lys Ala Asp Asp Lys Ile 
    2450                2455                2460                
Tyr Phe Gly Gly Leu Pro Thr Leu Arg Asn Leu Ser Met Lys Ala Arg 
2465                2470                2475                2480
Pro Glu Val Asn Val Lys Lys Tyr Ser Gly Cys Leu Lys Asp Ile Glu 
                2485                2490                2495    
Ile Ser Arg Thr Pro Tyr Asn Ile Leu Ser Ser Pro Asp Tyr Val Gly 
            2500                2505                2510        
Val Thr Lys Gly Cys Ser Leu Glu Asn Val Tyr Thr Val Ser Phe Pro 
        2515                2520                2525            
Lys Pro Gly Phe Val Glu Leu Ala Ala Val Ser Ile Asp Val Gly Thr 
    2530                2535                2540                
Glu Ile Asn Leu Ser Phe Ser Thr Arg Asn Glu Ser Gly Ile Ile Leu 
2545                2550                2555                2560
Leu Gly Ser Gly Gly Thr Leu Thr Pro Pro Arg Arg Lys Arg Arg Gln 
                2565                2570                2575    
Thr Thr Gln Ala Tyr Tyr Ala Ile Phe Leu Asn Lys Gly Arg Leu Glu 
            2580                2585                2590        
Val His Leu Ser Ser Gly Thr Arg Thr Met Arg Lys Ile Val Ile Lys 
        2595                2600                2605            
Pro Glu Pro Asn Leu Phe His Asp Gly Arg Glu His Ser Val His Val 
    2610                2615                2620                
Glu Arg Thr Arg Gly Ile Phe Thr Val Gln Ile Asp Glu Asp Arg Arg 
2625                2630                2635                2640
His Met Gln Asn Leu Thr Glu Glu Gln Pro Ile Glu Val Lys Lys Leu 
                2645                2650                2655    
Phe Val Gly Gly Ala Pro Pro Glu Phe Gln Pro Ser Pro Leu Arg Asn 
            2660                2665                2670        
Ile Pro Ala Phe Gln Gly Cys Val Trp Asn Leu Val Ile Asn Ser Ile 
        2675                2680                2685            
Pro Met Asp Phe Ala Gln Pro Ile Ala Phe Lys Asn Ala Asp Ile Gly 
    2690                2695                2700                
Arg Cys Thr Tyr Gln Lys Pro Arg Glu Asp Glu Ser Glu Ala Val Pro 
2705                2710                2715                2720
Ala Glu Val Ile Val Gln Pro Gln Pro Val Pro Thr Pro Ala Phe Pro 
                2725                2730                2735    
Phe Pro Ala Pro Thr Met Val His Gly Pro Cys Val Ala Glu Ser Glu 
            2740                2745                2750        
Pro Ala Leu Leu Thr Gly Ser Lys Gln Phe Gly Leu Ser Arg Asn Ser 
        2755                2760                2765            
His Ile Ala Ile Ala Phe Asp Asp Thr Lys Val Lys Asn Arg Leu Thr 
    2770                2775                2780                
Ile Glu Leu Glu Val Arg Thr Glu Ala Glu Ser Gly Leu Leu Phe Tyr 
2785                2790                2795                2800
Met Ala Arg Ile Asn His Ala Asp Phe Ala Thr Val Gln Leu Arg Asn 
                2805                2810                2815    
Gly Phe Pro Tyr Phe Ser Tyr Asp Leu Gly Ser Gly Asp Thr Ser Thr 
            2820                2825                2830        
Met Ile Pro Thr Lys Ile Asn Asp Gly Gln Trp His Lys Ile Lys Ile 
        2835                2840                2845            
Val Arg Val Lys Gln Glu Gly Ile Leu Tyr Val Asp Asp Ala Ser Ser 
    2850                2855                2860                
Gln Thr Ile Ser Pro Lys Lys Ala Asp Ile Leu Asp Val Val Gly Ile 
2865                2870                2875                2880
Leu Tyr Val Gly Gly Leu Pro Ile Asn Tyr Thr Thr Arg Arg Ile Gly 
                2885                2890                2895    
Pro Val Thr Tyr Ser Leu Asp Gly Cys Val Arg Asn Leu His Met Glu 
            2900                2905                2910        
Gln Ala Pro Val Asp Leu Asp Gln Pro Thr Ser Ser Phe His Val Gly 
        2915                2920                2925            
Thr Cys Phe Ala Asn Ala Glu Ser Gly Thr Tyr Phe Asp Gly Thr Gly 
    2930                2935                2940                
Phe Ala Lys Ala Val Gly Gly Phe Lys Val Gly Leu Asp Leu Leu Val 
2945                2950                2955                2960
Glu Phe Glu Phe Arg Thr Thr Arg Pro Thr Gly Val Leu Leu Gly Val 
                2965                2970                2975    
Ser Ser Gln Lys Met Asp Gly Met Gly Ile Glu Met Ile Asp Glu Lys 
            2980                2985                2990        
Leu Met Phe His Val Asp Asn Gly Ala Gly Arg Phe Thr Ala Ile Tyr 
        2995                3000                3005            
Asp Ala Gly Ile Pro Gly His Met Cys Asn Gly Gln Trp His Lys Val 
    3010                3015                3020                
Thr Ala Lys Lys Ile Lys Asn Arg Leu Glu Leu Val Val Asp Gly Asn 
3025                3030                3035                3040
Gln Val Asp Ala Gln Ser Pro Asn Ser Ala Ser Thr Ser Ala Asp Thr 
                3045                3050                3055    
Asn Asp Pro Val Phe Val Gly Gly Phe Pro Gly Gly Leu Asn Gln Phe 
            3060                3065                3070        
Gly Leu Thr Thr Asn Ile Arg Phe Arg Gly Cys Ile Arg Ser Leu Lys 
        3075                3080                3085            
Leu Thr Lys Gly Thr Gly Lys Pro Leu Glu Val Asn Phe Ala Lys Ala 
    3090                3095                3100                
Leu Glu Leu Arg Gly Val Gln Pro Val Ser Cys Pro Thr 
3105                3110                3115        

<210> 99
<211> 9353
<212> DNA
<213> mus musculus


<400> 99
atgcctgcgg ccaccgccgg gatcctcttg ctcctgctct tggggacgct cgaaggctcc       60

cagactcagc ggcgacagtc ccaagcgcat caacagagag gtttatttcc tgctgtcctg      120

aatcttgctt cgaatgcact catcacaacc aatgctacat gtggggaaaa aggacccgag      180

atgtactgca agttggtgga acatgtcccc gggcagcctg tgaggaaccc tcagtgccga      240

atctgcaatc agaacagcag caatccatac cagaggcacc cgattacgaa tgctattgat      300

ggcaagaaca catggtggca gagtcccagt atcaagaatg gagtggaata ccattatgtg      360

acaattactc tggatttaca gcaggtgttc cagattgcct acgtaattgt gaaggcagcc      420

aattcccctc ggcctggaaa ctggattttg gaacgttccc tggatgacgt ggagtacaaa      480

ccctggcagt atcatgcggt gacagacacg gagtgcctga ccctctacaa tatctatccc      540

cgcactggac caccatccta cgccaaagat gatgaggtca tctgcacttc attttattcg      600

aagatccacc ctttagaaaa tggagagatt cacatttctt tgatcaatgg gagaccaagt      660

gctgatgacc cctcccctga actcctggaa ttcacctctg ctcgctacat tcgcctgaga      720

tttcagagga tccgcacctt gaatgcagac ttgatgatgt ttgctcacaa agaccccaga      780

gaaatcgatc ccattgtcac acgaagatat tactattctg tcaaggatat ttcagttggc      840

gggatgtgca tctgttatgg tcatgcccgg gcttgtccac ttgaccctgc aacaaataaa      900

tcacgctgtg agtgtgaaca taacacctgt ggggaaagct gtgacaggtg ctgtccagga      960

ttccatcaga agccttggag agctggaacc ttcctcacca agtctgagtg tgaagcatgc     1020

aattgtcacg gaaaagctga ggaatgctat tatgatgaaa ctgttgctag cagaaatcta     1080

agtttaaata tacatgggaa gtacatcgga gggggtgtgt gcatcaactg cacacataac     1140

acggctggga taaattgtga gacatgtgtt gatggattct tcagaccaaa aggggtatca     1200

ccaaattatc caagaccatg ccagccatgt cactgtgatc caactggctc ccttagtgaa     1260

gtctgtgtca aagatgagaa atatgcccag cgagggttga aacctggatc ctgtcactgc     1320

aaaactggct ttggaggcgt gaactgtgat cgctgtgtca ggggttacca tggttaccca     1380

gactgccaac cctgtaactg tagtggcttg gggagcacaa acgaggaccc ttgcgttggg     1440

ccctgtagct gtaaggagaa tgttgaaggt gaagactgta gtcgttgcaa atctggtttc     1500

ttcaacttgc aagaagataa tcagaaaggc tgtgaggagt gtttctgttc aggagtatca     1560

aacagatgtc agagttccta ctggacctat gggaatattc aagacatgcg tggttggtat     1620

ctcacagacc tctctggccg cattcggatg gctccccagc ttgataaccc tgactcacct     1680

cagcagatca gcatcagtaa ctctgaggcc cggaaatccc tgcttgatgg ttactactgg     1740

agtgcaccgc ctccatatct gggaaacaga cttccagctg ttggaggaca gttgtcattt     1800

accatctcat atgacctcga agaagaggaa gacgatacag aaaaaatcct tcagctgatg     1860

attatctttg agggaaatga cttaagaatc agcacagcgt ataaggaggt gtacttagag     1920

ccatctgaag aacacattga ggaggtgtca ctcaaagaag aggcctttac tatacatgga     1980

acaaatttgc cagtcactag aaaagatttc atgattgttc tcacaaattt ggagagagtc     2040

cttatgcaaa tcacatacaa cttagggatg gacgccatct tcaggctgag ttctgtcaat     2100

cttgaatctg ctgtccctta tcctactgat agacgtattg caactgatgt ggaagtttgc     2160

cagtgtccac ctgggtacag tggcagctct tgtgaaacat gttggcctag gcaccgaaga     2220

gttaacggca ccatttttgg tggcatttgt gaaccatgtc agtgctttgc tcatgcagaa     2280

gcctgtgatg acatcacagg agaatgtctg aactgtaagg atcacacagg tgggccgtac     2340

tgcaatgaat gtctccctgg tttctatggt gatcctactc gaggaagccc tgaagactgt     2400

cagccctgtg cctgtccact caatatccca tcaaataact ttagtccaac atgccattta     2460

gaccggagtc tgggattgat ctgtgacgag tgtcctattg ggtacacagg accgcgctgt     2520

gagaggtgtg cagaaggcta ttttggacaa ccttccatac ctggaggatc atgtcagcca     2580

tgccaatgca atgacaacct tgactactcc atccctggca gctgtgacag cctgtctggc     2640

tcctgtctga tttgtaagcc aggtacaaca ggccggtact gtgagctctg tgctgatggg     2700

tattttggag acgcggttaa tgcaaagaac tgtcaaccat gccgttgtaa tatcaatggc     2760

tccttctcag agatttgtca cactagaact gggcaatgtg agtgcagacc caatgtgcag     2820

gggcggcact gtgacgagtg taagcctgaa acctttggcc tgcaactggg aaggggttgt     2880

ctgccctgca actgcaattc ttttgggtct aagtcctttg actgtgaagc aagtgggcag     2940

tgctggtgcc agcctggagt agcagggaag aaatgtgacc gttgtgccca tggctacttc     3000

aacttccaag aaggaggctg catagcttgt gactgttctc atctgggcaa caactgtgac     3060

ccaaaaactg gccaatgcat ttgcccaccc aataccactg gagaaaagtg ttctgagtgt     3120

cttcccaaca cctggggtca cagcattgtc accggctgta aggtttgtaa ctgcagcact     3180

gtggggtcct tggcttctca gtgcaatgta aacacgggcc agtgcagctg tcatccaaaa     3240

ttctctggta tgaaatgctc agagtgcagc cgaggtcact ggaactatcc tctctgcact     3300

ctatgtgact gcttccttcc agggacagat gccacgactt gtgatctgga gactaggaaa     3360

tgctcctgta gtgatcaaac tggacagtgc agctgtaagg tgaatgtgga aggcgtccac     3420

tgtgacaggt gccggcctgg caaatttgga ctagatgcca agaacccact tggctgcagc     3480

agctgctact gctttggagt tactagtcaa tgctctgaag caaaggggct gatccgtacg     3540

tgggtgactt tgagtgatga acagaccatt ctacctctgg tggatgaggc cctgcagcac     3600

acgactacca aaggcattgc tttccagaaa ccagagattg ttgcaaagat ggatgaagtc     3660

aggcaagagc tccatttgga acctttttac tggaaactcc cacaacaatt tgaagggaaa     3720

aagttgatgg cttatggtgg caaactcaag tatgccatct attttgaggc tcgggatgag     3780

acaggctttg ccacatataa acctcaagtt atcattcgag gtggaactcc tactcatgct     3840

agaattatta ccagacacat ggctgcccct ctcattggcc agttgacacg gcatgaaata     3900

gaaatgacag agaaagaatg gaaatattat ggtgatgatc ctcgaatcag tagaactgtg     3960

acccgtgaag acttcttgga tatactatat gatattcact atatccttat caaggctact     4020

tatggaaacg ttgtgagaca aagccgcatt tctgaaatct ccatggaagt agccgaacca     4080

ggacatgtat tagcagggag cccaccagca cacttgatag aaagatgcga ttgccctcct     4140

ggctattctg gcttgtcttg tgagacgtgt gcaccaggat tttatcgact tcgttctgaa     4200

ccaggtggcc gcactcctgg accaacctta ggaacctgtg ttccctgcca atgtaatgga     4260

cacagcagtc agtgtgatcc tgagacctca gtatgccaga attgtcagca tcacactgct     4320

ggtgacttct gtgagcgctg tgcccttggc tactatggaa tcgtcagggg attgccaaat     4380

gactgtcaac catgtgcttg tcctctgatt tcgcccagca acaatttcag cccctcttgt     4440

gtattggaag gtctggaaga ttaccgttgc accgcctgcc caaggggcta tgaaggacag     4500

tactgtgaaa ggtgtgcccc aggctatact ggcagcccaa gcagccccgg aggctcctgc     4560

caagaatgtg agtgtgaccc ttatggctcc ctaccggttc cctgtgaccg ggtcacggga     4620

ctctgcacgt gccgccctgg agccacagga aggaagtgtg atggctgcga gcactggcat     4680

gcacgcgagg gtgcagagtg tgtcttttgt ggagacgagt gtacaggcct tcttcttggt     4740

gacctggctc gtctagagca gatgaccatg aacatcaacc tcacgggccc actgcctgct     4800

ccatataaaa ttctgtatgg tcttgaaaat acaactcagg aactcaagca cctgctatca     4860

ccgcaacggg caccagagag gctcattcag ttggcagagg gcaacgtgaa cacacttgtg     4920

atggaaacaa atgagctgct aaccagagca accaaagtga cagcagatgg tgagcaaaca     4980

ggacaagatg ctgagaggac caactccaga gcagaatcct tggaagaatt cattaaaggg     5040

cttgtccagg atgctgaagc cataaatgaa aaagctgtac aactaaatga aaccttagga     5100

aatcaagata agacagcaga gagaaacttg gaggagcttc aaaaggaaat cgaccggatg     5160

ctgaaggaac tgagaagtaa agatcttcaa acacagaagg aagttgctga ggatgagctc     5220

gtggcagcag aaggccttct gaagagagta aacaagctgt ttggagagcc cagagcccag     5280

aatgaagata tggaaaagga tctccagcag aaactggcag agtacaagaa caaacttgat     5340

gatgcttggg atctattgag agaagccact gataaaactc gagatgctaa tcgtttgtct     5400

gctgccaatc aaaaaaacat gaccatactg gagacaaaga aggaggctat tgaaggtagc     5460

aaacgacaaa tagagaacac tttaaaggaa ggcaatgaca tccttgatga agccaatcga     5520

ctcttaggtg aaatcaactc agtcatagat tatgtcgacg acattaaaac taagttgcca     5580

ccaatgtccg aggagctgag tgacaaaata gatgacctcg cccaggaaat aaaggacaga     5640

aggcttgctg agaaggtgtt ccaggctgag agccatgctg ctcagctgaa cgactcgtct     5700

gctgtacttg atggaatcct ggatgaggct aagaacatct ctttcaatgc cacggcagcc     5760

ttcagagctt acagtaatat taaagactac attgatgaag ctgagaaagt ggccagagaa     5820

gccaaagagc ttgcccaagg ggctacaaaa ctggcaacaa gtcctcaggg cttattaaaa     5880

gaagatgcca aaggctccct tcagaaaagc ttcaggatcc tcaatgaagc caagaagcta     5940

gcaaacgatg tgaaaggaaa tcacaatgat ctaaatgacc tgaaaaccag gttagaaact     6000

gctgacctta gaaacagtgg acttctagga gctctaaatg acaccatgga caagttatca     6060

gccattacaa atgacacggc tgctaaactg caggctgtca aagagaaagc cagagaagcc     6120

aatgacacag caaaagctgt cctggcccag gttaaggacc tgcatcagaa cctagatggc     6180

ctgaagcaaa actacaataa actggcagac agcgtggcca aaacgaacgc tgtggtgaaa     6240

gatccttcca aaaacaaaat cattgcagat gcaggcactt ccgtgagaaa tctagaacag     6300

gaagctgacc ggctaatcga caaactcaag cccatcaagg agcttgagga caacctaaag     6360

aaaaacattt ctgaaataaa ggaactgatc aaccaagctc ggaaacaagc taactctatc     6420

aaagtatctg tttcttcggg aggtgactgt gttcgaacat acaggccaga aatcaagaaa     6480

ggaagctaca ataacatcgt tgtccatgtc aagaccgctg ttgccgacaa cctccttttt     6540

tatcttggaa gtgccaaatt tattgacttt cttgctatag aaatgcgcaa aggcaaagtc     6600

agcttcctct gggatgttgg ctctggagtt ggccgagtag agtatccaga cttgaccatc     6660

gacgactcct attggtaccg tattgaagca tcaagaacgg gaagaaatgg atctatttct     6720

gtgagagctt tagatggacc caaagccagt atggtaccca gcacctacca ttcagtgtct     6780

cctcccgggt acactatcct agatgtggat gcaaatgcaa tgctgtttgt tggtggcctg     6840

accggaaaaa taaagaaggc cgatgctgta cgtgtgatca ccttcaccgg ctgtatggga     6900

gaaacatact ttgacaacaa acctataggt ttatggaact tccgggagaa agaaggcgac     6960

tgtaagggat gtactgtcag cccacaagtg gaagatagtg aggggactat tcagtttgat     7020

ggtgaaggct atgcattagt gagccgccct atccgctggt accccaacat ctccacagtc     7080

atgttcaagt tccggacatt ttcatcaagt gctctcctga tgtatcttgc cacacgagac     7140

ctgaaagatt tcatgagtgt agagctcagt gatggacatg tgaaagtcag ctatgacctg     7200

ggctcaggaa tgacttccgt tgtcagcaat caaaaccata atgatgggaa atggaaagca     7260

ttcacgctgt cgcggattca gaaacaagcc aacatatcga ttgtcgacat cgattctaac     7320

caggaggaga atgtagctac ttcatcttct ggaaacaact ttggtcttga cttgaaagca     7380

gatgacaaaa tatattttgg tggcctgcca actctgagaa acttgagtat gaaagcaagg     7440

ccagaagtca atgtgaagaa atactccggc tgcctcaaag atattgaaat ttcaagaaca     7500

ccttacaata tactcagcag ccctgattat gttggtgtga ccaaaggctg ttcactggag     7560

aatgtttata cagttagttt ccccaagcct ggttttgtgg agcttgccgc tgtgtctatt     7620

gatgttggaa cagaaatcaa tctgtccttt agtaccagga acgagtctgg gatcattctc     7680

ttgggaagtg gagggacact cacaccaccc aggagaaaac ggagacaaac cacacaggct     7740

tattatgcca tattcctcaa caagggccgc ttggaagtgc atctctcctc ggggacacgg     7800

acaatgagga aaattgtcat caaaccggag ccaaatttgt ttcatgatgg gagagaacat     7860

tctgtccacg tagaaagaac cagaggcatc ttcactgttc aaattgatga agacagaaga     7920

catatgcaaa acctgacaga ggaacagccc atcgaagtga aaaagctctt tgtcgggggt     7980

gctcctcctg aatttcagcc ctccccactc aggaatattc cggcctttca aggctgtgtg     8040

tggaaccttg ttattaactc catccccatg gactttgcgc agcctatagc cttcaaaaat     8100

gccgacattg gccgctgtac ctatcaaaag ccccgggaag atgagagtga agcagttcca     8160

gctgaagtta ttgtccagcc tcagccggtg cccacccctg ccttcccttt cccggccccc     8220

accatggtgc atggcccttg tgttgcagaa tcagaaccag ctcttctgac agggagcaag     8280

cagtttgggc tttccagaaa cagccacatt gcaattgctt ttgatgacac caaagttaaa     8340

aatcgcctca ccattgagct ggaggtacga actgaagctg aatcaggctt gctcttctac     8400

atggctcgga tcaatcatgc tgattttgct actgttcagc tgaggaatgg cttcccgtac     8460

ttcagttatg atttggggag tggggacaca agcaccatga tccccacaaa aatcaacgat     8520

ggccagtggc acaagattaa gattgtgaga gtgaagcagg agggaattct ttatgtggat     8580

gatgcctcca gccaaaccat cagtcccaag aaagctgaca tcctggatgt cgtggggatt     8640

ctgtatgtcg gtggattgcc gatcaactat accacacgca gaattggtcc agtgacttac     8700

agcctggatg gctgtgttag gaatcttcac atggaacaag cccctgttga tctggaccag     8760

cctacctcca gctttcacgt tgggacatgc tttgcgaatg cagagagtgg gacttacttt     8820

gatggaaccg gttttgctaa agcagttggt gggttcaaag ttggattgga ccttcttgtg     8880

gaatttgaat tccgtaccac aagacccact ggggtcctcc tgggagtcag cagtcagaag     8940

atggatggaa tgggtattga aatgatcgac gagaagctta tgttccacgt ggataatggc     9000

gctggccgat tcactgcaat ctatgatgct gggatcccag gccacatgtg caatggacag     9060

tggcataaag tcactgccaa gaagatcaaa aaccgtcttg agctggtggt agatgggaac     9120

caggtggatg cccagagccc aaactcagca tcgacatcag ctgatacaaa cgaccctgtt     9180

tttgttggcg gtttcccagg tggcctcaat cagtttggcc tgaccaccaa cattaggttc     9240

cgaggctgca tccgatctct gaagctcacc aaaggcactg gcaaaccgct ggaggttaat     9300

tttgccaagg ccctggaact gaggggtgtt caacctgtat catgcccgac tac            9353


<210> 100
<211> 21
<212> DNA
<213> artificial sequence


<220> 
<223> SYBR-Mm_Tfrc-F qPCR

<400> 100
tgggcactag attggatacc t                                              21


<210> 101
<211> 20
<212> DNA
<213> artificial sequence


<220> 
<223> SYBR-Mm_Tfrc-R qPCR

<400> 101
atgagctgac cagccacttc                                                20


<210> 102
<211> 19
<212> DNA
<213> artificial sequence


<220> 
<223> SYBR-RFP-F qPCR

<400> 102
atggccagct ccgaggatg                                                 19


<210> 103
<211> 22
<212> DNA
<213> artificial sequence


<220> 
<223> SYBR-RFP-R qPCR

<400> 103
gaactgaggg ctcagaatat cc                                             22


<210> 104
<211> 20
<212> DNA
<213> artificial sequence


<220> 
<223> SYBR-hLama F qPCR

<400> 104
tagaacatgt ccctgggcag                                                20


<210> 105
<211> 20
<212> DNA
<213> artificial sequence


<220> 
<223> SYBR-hLama r qPCR

<400> 105
atacgcgatc tggaacacct                                                20


<210> 106
<211> 20
<212> DNA
<213> artificial sequence


<220> 
<223> SYBR-Mm-GAPDH-F qPCR

<400> 106
cgtcccgtag acaaaatggt                                                20


<210> 107
<211> 20
<212> DNA
<213> artificial sequence


<220> 
<223> SYBR-Mm-GAPDH-R qPCR

<400> 107
tggaagatgg tgatgggctt                                                20


<210> 108
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac R275A/N347S/K375A/D450N/S592G

<400> 108
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Ala Gly Arg Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Ser Trp Phe Thr Ser Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Arg Ser Asn Ala Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asn Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560 
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Ser Ala Ser Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Gly 
            580                 585                 590         
Cys Phe 
        

<210> 109
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac N347S/D450N/T560A/S573A/F594L

<400> 109
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Arg Gly Arg Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Ser Trp Phe Thr Ser Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asn Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Ala 
545                 550                 555                 560 
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Ser Ala Ala Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser 
            580                 585                 590         
Cys Leu 
        

<210> 110
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac N347A/D450N

<400> 110
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Arg Gly Arg Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Ser Trp Phe Thr Ser Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asn Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560 
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Ser Ala Ser Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser 
            580                 585                 590         
Cys Phe 
        

<210> 111
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac R275A/N347S/R372A/D450N/T560A/F594L

<400> 111
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Ala Gly Arg Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Ser Trp Phe Thr Ser Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Ala Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asn Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Ala 
545                 550                 555                 560 
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Ser Ala Ser Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser 
            580                 585                 590         
Cys Leu 
        

<210> 112
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac
      R202K/R275A/N347S/R372A/D450N/T560A/F594L

<400> 112
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Lys Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Ala Gly Arg Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Ser Trp Phe Thr Ser Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Ala Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asn Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Ala 
545                 550                 555                 560 
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Ser Ala Ser Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser 
            580                 585                 590         
Cys Leu 
        

<210> 113
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac
      R275A/R277A/N347S/R372A/D450N/T560A/S564P/F594L

<400> 113
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Ala Gly Ala Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Ser Trp Phe Thr Ser Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Ala Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asn Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Ala 
545                 550                 555                 560 
Tyr Cys Pro Pro Lys Ile Arg Arg Lys Ala Ser Ala Ser Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser 
            580                 585                 590         
Cys Leu 
        

<210> 114
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<221> SITE
<222> 1..5
<223> wherein the sequence can be repeated 1 to 50 times

<220> 
<223> Linker

<400> 114
Gly Gly Gly Gly Ser 
1               5   

<210> 115
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<221> SITE
<222> 1..5
<223> wherein the sequence can be repeated 1 to 50 times

<220> 
<223> Linker

<400> 115
Glu Ala Ala Ala Lys 
1               5   

<210> 116
<211> 3210
<212> PRT
<213> Artificial Sequence


<220> 
<223> laminin subunit α2 isoform X1

<400> 116
Met Pro Gly Ala Ala Gly Val Leu Leu Leu Leu Leu Leu Ser Gly Gly 
1               5                   10                  15      
Leu Gly Gly Val Gln Ala Gln Arg Pro Gln Gln Gln Arg Gln Ser Gln 
            20                  25                  30          
Ala His Gln Gln Arg Gly Leu Phe Pro Ala Val Leu Asn Leu Ala Ser 
        35                  40                  45              
Asn Ala Leu Ile Thr Thr Asn Ala Thr Cys Gly Glu Lys Gly Pro Glu 
    50                  55                  60                  
Met Tyr Cys Lys Leu Val Glu His Val Pro Gly Gln Pro Val Arg Asn 
65                  70                  75                  80  
Pro Gln Cys Arg Ile Cys Asn Gln Asn Ser Ser Asn Pro Asn Gln Arg 
                85                  90                  95      
His Pro Ile Thr Asn Ala Ile Asp Gly Lys Asn Thr Trp Trp Gln Ser 
            100                 105                 110         
Pro Ser Ile Lys Asn Gly Ile Glu Tyr His Tyr Val Thr Ile Thr Leu 
        115                 120                 125             
Asp Leu Gln Gln Val Phe Gln Ile Ala Tyr Val Ile Val Lys Ala Ala 
    130                 135                 140                 
Asn Ser Pro Arg Pro Gly Asn Trp Ile Leu Glu Arg Ser Leu Asp Asp 
145                 150                 155                 160 
Val Glu Tyr Lys Pro Trp Gln Tyr His Ala Val Thr Asp Thr Glu Cys 
                165                 170                 175     
Leu Thr Leu Tyr Asn Ile Tyr Pro Arg Thr Gly Pro Pro Ser Tyr Ala 
            180                 185                 190         
Lys Asp Asp Glu Val Ile Cys Thr Ser Phe Tyr Ser Lys Ile His Pro 
        195                 200                 205             
Leu Glu Asn Gly Glu Ile His Ile Ser Leu Ile Asn Gly Arg Pro Ser 
    210                 215                 220                 
Ala Asp Asp Pro Ser Pro Glu Leu Leu Glu Phe Thr Ser Ala Arg Tyr 
225                 230                 235                 240 
Ile Arg Leu Arg Phe Gln Arg Ile Arg Thr Leu Asn Ala Asp Leu Met 
                245                 250                 255     
Met Phe Ala His Lys Asp Pro Arg Glu Ile Asp Pro Ile Val Thr Arg 
            260                 265                 270         
Arg Tyr Tyr Tyr Ser Val Lys Asp Ile Ser Val Gly Gly Met Cys Ile 
        275                 280                 285             
Cys Tyr Gly His Ala Arg Ala Cys Pro Leu Asp Pro Ala Thr Asn Lys 
    290                 295                 300                 
Ser Arg Cys Glu Cys Glu His Asn Thr Cys Gly Asp Ser Cys Asp Gln 
305                 310                 315                 320 
Cys Cys Pro Gly Phe His Gln Lys Pro Trp Arg Ala Gly Thr Phe Leu 
                325                 330                 335     
Thr Lys Thr Glu Cys Glu Ala Cys Asn Cys His Gly Lys Ala Glu Glu 
            340                 345                 350         
Cys Tyr Tyr Asp Glu Asn Val Ala Arg Arg Asn Leu Ser Leu Asn Ile 
        355                 360                 365             
Arg Gly Lys Tyr Ile Gly Gly Gly Val Cys Ile Asn Cys Thr Gln Asn 
    370                 375                 380                 
Thr Ala Gly Ile Asn Cys Glu Thr Cys Thr Asp Gly Phe Phe Arg Pro 
385                 390                 395                 400 
Lys Gly Val Ser Pro Asn Tyr Pro Arg Pro Cys Gln Pro Cys His Cys 
                405                 410                 415     
Asp Pro Ile Gly Ser Leu Asn Glu Val Cys Val Lys Asp Glu Lys His 
            420                 425                 430         
Ala Arg Arg Gly Leu Ala Pro Gly Ser Cys His Cys Lys Thr Gly Phe 
        435                 440                 445             
Gly Gly Val Ser Cys Asp Arg Cys Ala Arg Gly Tyr Thr Gly Tyr Pro 
    450                 455                 460                 
Asp Cys Lys Ala Cys Asn Cys Ser Gly Leu Gly Ser Lys Asn Glu Asp 
465                 470                 475                 480 
Pro Cys Phe Gly Pro Cys Ile Cys Lys Glu Asn Val Glu Gly Gly Asp 
                485                 490                 495     
Cys Ser Arg Cys Lys Ser Gly Phe Phe Asn Leu Gln Glu Asp Asn Trp 
            500                 505                 510         
Lys Gly Cys Asp Glu Cys Phe Cys Ser Gly Val Ser Asn Arg Cys Gln 
        515                 520                 525             
Ser Ser Tyr Trp Thr Tyr Gly Lys Ile Gln Asp Met Ser Gly Trp Tyr 
    530                 535                 540                 
Leu Thr Asp Leu Pro Gly Arg Ile Arg Val Ala Pro Gln Gln Asp Asp 
545                 550                 555                 560 
Leu Asp Ser Pro Gln Gln Ile Ser Ile Ser Asn Ala Glu Ala Arg Gln 
                565                 570                 575     
Ala Leu Pro His Ser Tyr Tyr Trp Ser Ala Pro Ala Pro Tyr Leu Gly 
            580                 585                 590         
Asn Lys Leu Pro Ala Val Gly Gly Gln Leu Thr Phe Thr Ile Ser Tyr 
        595                 600                 605             
Asp Leu Glu Glu Glu Glu Glu Asp Thr Glu Arg Val Leu Gln Leu Met 
    610                 615                 620                 
Ile Ile Leu Glu Gly Asn Asp Leu Ser Ile Ser Thr Ala Gln Asp Glu 
625                 630                 635                 640 
Val Tyr Leu His Pro Ser Glu Glu His Thr Asn Val Leu Leu Leu Lys 
                645                 650                 655     
Glu Glu Ser Phe Thr Ile His Gly Thr His Phe Pro Val Arg Arg Lys 
            660                 665                 670         
Glu Phe Met Thr Val Leu Ala Asn Leu Lys Arg Val Leu Leu Gln Ile 
        675                 680                 685             
Thr Tyr Ser Phe Gly Met Asp Ala Ile Phe Arg Leu Ser Ser Val Asn 
    690                 695                 700                 
Leu Glu Ser Ala Val Ser Tyr Pro Thr Asp Gly Ser Ile Ala Ala Ala 
705                 710                 715                 720 
Val Glu Val Cys Gln Cys Pro Pro Gly Tyr Thr Gly Ser Ser Cys Glu 
                725                 730                 735     
Ser Cys Trp Pro Arg His Arg Arg Val Asn Gly Thr Ile Phe Gly Gly 
            740                 745                 750         
Ile Cys Glu Pro Cys Gln Cys Phe Gly His Ala Glu Ser Cys Asp Asp 
        755                 760                 765             
Val Thr Gly Glu Cys Leu Asn Cys Lys Asp His Thr Gly Gly Pro Tyr 
    770                 775                 780                 
Cys Asp Lys Cys Leu Pro Gly Phe Tyr Gly Glu Pro Thr Lys Gly Thr 
785                 790                 795                 800 
Ser Glu Asp Cys Gln Pro Cys Ala Cys Pro Leu Asn Ile Pro Ser Asn 
                805                 810                 815     
Asn Phe Ser Pro Thr Cys His Leu Asp Arg Ser Leu Gly Leu Ile Cys 
            820                 825                 830         
Asp Gly Cys Pro Val Gly Tyr Thr Gly Pro Arg Cys Glu Arg Cys Ala 
        835                 840                 845             
Glu Gly Tyr Phe Gly Gln Pro Ser Val Pro Gly Gly Ser Cys Gln Pro 
    850                 855                 860                 
Cys Gln Cys Asn Asp Asn Leu Asp Phe Ser Ile Pro Gly Ser Cys Asp 
865                 870                 875                 880 
Ser Leu Ser Gly Ser Cys Leu Ile Cys Lys Pro Gly Thr Thr Gly Arg 
                885                 890                 895     
Tyr Cys Glu Leu Cys Ala Asp Gly Tyr Phe Gly Asp Ala Val Asp Ala 
            900                 905                 910         
Lys Asn Cys Gln Pro Cys Arg Cys Asn Ala Gly Gly Ser Phe Ser Glu 
        915                 920                 925             
Val Cys His Ser Gln Thr Gly Gln Cys Glu Cys Arg Ala Asn Val Gln 
    930                 935                 940                 
Gly Gln Arg Cys Asp Lys Cys Lys Pro Asn Met Trp Arg Asp Pro Glu 
945                 950                 955                 960 
Lys Arg Phe Cys Val Leu Cys Asp Cys Asp Pro Val Gly Ser Val Ser 
                965                 970                 975     
Pro Gln Cys Asp Ile Thr Gly Arg Cys Val Cys Lys Ser Gly Phe Val 
            980                 985                 990         
Gly Lys Gln Cys Asn Leu Gly Arg Gln Val His Gln Gln Glu Glu Gln 
        995                 1000                1005            
Pro Arg Arg Ala Gln Arg Val Leu Gly Ser Pro Gln Arg Trp Ala Ile 
    1010                1015                1020                
Gly Ser Ser Ser Gly Cys Pro Arg Gly Ala Tyr Arg Ala Pro Ala Pro 
1025                1030                1035                1040
Ala Gly Thr Phe Gly Leu Gln Ser Ala Arg Gly Cys Val Pro Cys Asn 
                1045                1050                1055    
Cys Asn Ser Phe Gly Ser Lys Ser Phe Asp Cys Glu Glu Ser Gly Gln 
            1060                1065                1070        
Cys Trp Cys Gln Pro Gly Val Thr Gly Lys Lys Cys Asp Arg Cys Ala 
        1075                1080                1085            
His Gly Tyr Phe Asn Phe Gln Glu Gly Gly Cys Thr Ala Cys Glu Cys 
    1090                1095                1100                
Ser His Leu Gly Asn Asn Cys Asp Pro Lys Thr Gly Arg Cys Ile Cys 
1105                1110                1115                1120
Pro Pro Asn Thr Ile Gly Glu Lys Cys Ser Lys Cys Ala Pro Asn Thr 
                1125                1130                1135    
Trp Gly His Ser Ile Thr Thr Gly Cys Lys Ala Cys Asn Cys Ser Thr 
            1140                1145                1150        
Val Gly Ser Leu Asp Phe Gln Cys Asn Val Asn Thr Gly Gln Cys Asn 
        1155                1160                1165            
Cys His Pro Lys Phe Ser Gly Ala Lys Cys Thr Glu Cys Ser Arg Gly 
    1170                1175                1180                
His Trp Asn Tyr Pro Arg Cys Asn Leu Cys Asp Cys Phe Leu Pro Gly 
1185                1190                1195                1200
Thr Asp Ala Thr Thr Cys Asp Ser Glu Thr Lys Lys Cys Ser Cys Ser 
                1205                1210                1215    
Asp Gln Thr Gly Gln Cys Thr Cys Lys Val Asn Val Glu Gly Ile His 
            1220                1225                1230        
Cys Asp Arg Cys Arg Pro Gly Lys Phe Gly Leu Asp Ala Lys Asn Pro 
        1235                1240                1245            
Leu Gly Cys Ser Ser Cys Tyr Cys Phe Gly Thr Thr Thr Gln Cys Ser 
    1250                1255                1260                
Glu Ala Lys Gly Leu Ile Arg Thr Trp Val Thr Leu Lys Ala Glu Gln 
1265                1270                1275                1280
Thr Ile Leu Pro Leu Val Asp Glu Ala Leu Gln His Thr Thr Thr Lys 
                1285                1290                1295    
Gly Ile Val Phe Gln His Pro Glu Ile Val Ala His Met Asp Leu Met 
            1300                1305                1310        
Arg Glu Asp Leu His Leu Glu Pro Phe Tyr Trp Lys Leu Pro Glu Gln 
        1315                1320                1325            
Phe Glu Gly Lys Lys Leu Met Ala Tyr Gly Gly Lys Leu Lys Tyr Ala 
    1330                1335                1340                
Ile Tyr Phe Glu Ala Arg Glu Glu Thr Gly Phe Ser Thr Tyr Asn Pro 
1345                1350                1355                1360
Gln Val Ile Ile Arg Gly Gly Thr Pro Thr His Ala Arg Ile Ile Val 
                1365                1370                1375    
Arg His Met Ala Ala Pro Leu Ile Gly Gln Leu Thr Arg His Glu Ile 
            1380                1385                1390        
Glu Met Thr Glu Lys Glu Trp Lys Tyr Tyr Gly Asp Asp Pro Arg Val 
        1395                1400                1405            
His Arg Thr Val Thr Arg Glu Asp Phe Leu Asp Ile Leu Tyr Asp Ile 
    1410                1415                1420                
His Tyr Ile Leu Ile Lys Ala Thr Tyr Gly Asn Phe Met Arg Gln Ser 
1425                1430                1435                1440
Arg Ile Ser Glu Ile Ser Met Glu Val Ala Glu Gln Gly Arg Gly Thr 
                1445                1450                1455    
Thr Met Thr Pro Pro Ala Asp Leu Ile Glu Lys Cys Asp Cys Pro Leu 
            1460                1465                1470        
Gly Tyr Ser Gly Leu Ser Cys Glu Ala Cys Leu Pro Gly Phe Tyr Arg 
        1475                1480                1485            
Leu Arg Ser Gln Pro Gly Gly Arg Thr Pro Gly Pro Thr Leu Gly Thr 
    1490                1495                1500                
Cys Val Pro Cys Gln Cys Asn Gly His Ser Ser Leu Cys Asp Pro Glu 
1505                1510                1515                1520
Thr Ser Ile Cys Gln Asn Cys Gln His His Thr Ala Gly Asp Phe Cys 
                1525                1530                1535    
Glu Arg Cys Ala Leu Gly Tyr Tyr Gly Ile Val Lys Gly Leu Pro Asn 
            1540                1545                1550        
Asp Cys Gln Gln Cys Ala Cys Pro Leu Ile Ser Ser Ser Asn Asn Phe 
        1555                1560                1565            
Ser Pro Ser Cys Val Ala Glu Gly Leu Asp Asp Tyr Arg Cys Thr Ala 
    1570                1575                1580                
Cys Pro Arg Gly Tyr Glu Gly Gln Tyr Cys Glu Arg Cys Ala Pro Gly 
1585                1590                1595                1600
Tyr Thr Gly Ser Pro Gly Asn Pro Gly Gly Ser Cys Gln Glu Cys Glu 
                1605                1610                1615    
Cys Asp Pro Tyr Gly Ser Leu Pro Val Pro Cys Asp Pro Val Thr Gly 
            1620                1625                1630        
Phe Cys Thr Cys Arg Pro Gly Ala Thr Gly Arg Lys Cys Asp Gly Cys 
        1635                1640                1645            
Lys His Trp His Ala Arg Glu Gly Trp Glu Cys Val Phe Cys Gly Asp 
    1650                1655                1660                
Glu Cys Thr Gly Leu Leu Leu Gly Asp Leu Ala Arg Leu Glu Gln Met 
1665                1670                1675                1680
Val Met Ser Ile Asn Leu Thr Gly Pro Leu Pro Ala Pro Tyr Lys Met 
                1685                1690                1695    
Leu Tyr Gly Leu Glu Asn Met Thr Gln Glu Leu Lys His Leu Leu Ser 
            1700                1705                1710        
Pro Gln Arg Ala Pro Glu Arg Leu Ile Gln Leu Ala Glu Gly Asn Leu 
        1715                1720                1725            
Asn Thr Leu Val Thr Glu Met Asn Glu Leu Leu Thr Arg Ala Thr Lys 
    1730                1735                1740                
Val Thr Ala Asp Gly Glu Gln Thr Gly Gln Asp Ala Glu Arg Thr Asn 
1745                1750                1755                1760
Thr Arg Ala Lys Ser Leu Gly Glu Phe Ile Lys Glu Leu Ala Arg Asp 
                1765                1770                1775    
Ala Glu Ala Val Asn Glu Lys Ala Ile Lys Leu Asn Glu Thr Leu Gly 
            1780                1785                1790        
Thr Arg Asp Glu Ala Phe Glu Arg Asn Leu Glu Gly Leu Gln Lys Glu 
        1795                1800                1805            
Ile Asp Gln Met Ile Lys Glu Leu Arg Arg Lys Asn Leu Glu Thr Gln 
    1810                1815                1820                
Lys Glu Ile Ala Glu Asp Glu Leu Val Ala Ala Glu Ala Leu Leu Lys 
1825                1830                1835                1840
Lys Val Lys Lys Leu Phe Gly Glu Ser Arg Gly Glu Asn Glu Glu Met 
                1845                1850                1855    
Glu Lys Asp Leu Arg Glu Lys Leu Ala Asp Tyr Lys Asn Lys Val Asp 
            1860                1865                1870        
Asp Ala Trp Asp Leu Leu Arg Glu Ala Thr Asp Lys Ile Arg Glu Ala 
        1875                1880                1885            
Asn Arg Leu Phe Ala Val Asn Gln Lys Asn Met Thr Ala Leu Glu Lys 
    1890                1895                1900                
Lys Lys Glu Ala Val Glu Ser Gly Lys Arg Gln Ile Glu Asn Thr Leu 
1905                1910                1915                1920
Lys Glu Gly Asn Asp Ile Leu Asp Glu Ala Asn Arg Leu Ala Asp Glu 
                1925                1930                1935    
Ile Asn Ser Ile Ile Asp Tyr Val Glu Asp Ile Gln Thr Lys Leu Pro 
            1940                1945                1950        
Pro Met Ser Glu Glu Leu Asn Asp Lys Ile Asp Asp Leu Ser Gln Glu 
        1955                1960                1965            
Ile Lys Asp Arg Lys Leu Ala Glu Lys Val Ser Gln Ala Glu Ser His 
    1970                1975                1980                
Ala Ala Gln Leu Asn Asp Ser Ser Ala Val Leu Asp Gly Ile Leu Asp 
1985                1990                1995                2000
Glu Ala Lys Asn Ile Ser Phe Asn Ala Thr Ala Ala Phe Lys Ala Tyr 
                2005                2010                2015    
Ser Asn Ile Lys Asp Tyr Ile Asp Glu Ala Glu Lys Val Ala Lys Glu 
            2020                2025                2030        
Ala Lys Asp Leu Ala His Glu Ala Thr Lys Leu Ala Thr Gly Pro Arg 
        2035                2040                2045            
Gly Leu Leu Lys Glu Asp Ala Lys Gly Cys Leu Gln Lys Ser Phe Arg 
    2050                2055                2060                
Ile Leu Asn Glu Ala Lys Lys Leu Ala Asn Asp Val Lys Glu Asn Glu 
2065                2070                2075                2080
Asp His Leu Asn Gly Leu Lys Thr Arg Ile Glu Asn Ala Asp Ala Arg 
                2085                2090                2095    
Asn Gly Asp Leu Leu Arg Thr Leu Asn Asp Thr Leu Gly Lys Leu Ser 
            2100                2105                2110        
Ala Ile Pro Asn Asp Thr Ala Ala Lys Leu Gln Ala Val Lys Asp Lys 
        2115                2120                2125            
Ala Arg Gln Ala Asn Asp Thr Ala Lys Asp Val Leu Ala Gln Ile Thr 
    2130                2135                2140                
Glu Leu His Gln Asn Leu Asp Gly Leu Lys Lys Asn Tyr Asn Lys Leu 
2145                2150                2155                2160
Ala Asp Ser Val Ala Lys Thr Asn Ala Val Val Lys Asp Pro Ser Lys 
                2165                2170                2175    
Asn Lys Ile Ile Ala Asp Ala Asp Ala Thr Val Lys Asn Leu Glu Gln 
            2180                2185                2190        
Glu Ala Asp Arg Leu Ile Asp Lys Leu Lys Pro Ile Lys Glu Leu Glu 
        2195                2200                2205            
Asp Asn Leu Lys Lys Asn Ile Ser Glu Ile Lys Glu Leu Ile Asn Gln 
    2210                2215                2220                
Ala Arg Lys Gln Ala Asn Ser Ile Lys Val Ser Val Ser Ser Gly Gly 
2225                2230                2235                2240
Asp Cys Ile Arg Thr Tyr Lys Pro Glu Ile Lys Lys Gly Ser Tyr Asn 
                2245                2250                2255    
Asn Ile Val Val Asn Val Lys Thr Ala Val Ala Asp Asn Leu Leu Phe 
            2260                2265                2270        
Tyr Leu Gly Ser Ala Lys Phe Ile Asp Phe Leu Ala Ile Glu Met Arg 
        2275                2280                2285            
Lys Gly Lys Val Ser Phe Leu Trp Asp Val Gly Ser Gly Val Gly Arg 
    2290                2295                2300                
Val Glu Tyr Pro Asp Leu Thr Ile Asp Asp Ser Tyr Trp Tyr Arg Ile 
2305                2310                2315                2320
Val Ala Ser Arg Thr Gly Arg Asn Gly Thr Ile Ser Val Arg Ala Leu 
                2325                2330                2335    
Asp Gly Pro Lys Ala Ser Ile Val Pro Ser Thr His His Ser Thr Ser 
            2340                2345                2350        
Pro Pro Gly Tyr Thr Ile Leu Asp Val Asp Ala Asn Ala Met Leu Phe 
        2355                2360                2365            
Val Gly Gly Leu Thr Gly Lys Leu Lys Lys Ala Asp Ala Val Arg Val 
    2370                2375                2380                
Ile Thr Phe Thr Gly Cys Met Gly Glu Thr Tyr Phe Asp Asn Lys Pro 
2385                2390                2395                2400
Ile Gly Leu Trp Asn Phe Arg Glu Lys Glu Gly Asp Cys Lys Gly Cys 
                2405                2410                2415    
Thr Val Ser Pro Gln Val Glu Asp Ser Glu Gly Thr Ile Gln Phe Asp 
            2420                2425                2430        
Gly Glu Gly Tyr Ala Leu Val Ser Arg Pro Ile Arg Trp Tyr Pro Asn 
        2435                2440                2445            
Ile Ser Thr Val Met Phe Lys Phe Arg Thr Phe Ser Ser Ser Ala Leu 
    2450                2455                2460                
Leu Met Tyr Leu Ala Thr Arg Asp Leu Arg Asp Phe Met Ser Val Glu 
2465                2470                2475                2480
Leu Thr Asp Gly His Ile Lys Val Ser Tyr Asp Leu Gly Ser Gly Met 
                2485                2490                2495    
Ala Ser Val Val Ser Asn Gln Asn His Asn Asp Gly Lys Trp Lys Ser 
            2500                2505                2510        
Phe Thr Leu Ser Arg Ile Gln Lys Gln Ala Asn Ile Ser Ile Val Asp 
        2515                2520                2525            
Ile Asp Thr Asn Gln Glu Glu Asn Ile Ala Thr Ser Ser Ser Gly Asn 
    2530                2535                2540                
Asn Phe Gly Leu Asp Leu Lys Ala Asp Asp Lys Ile Tyr Phe Gly Gly 
2545                2550                2555                2560
Leu Pro Thr Leu Arg Asn Leu Ser Met Lys Ala Arg Pro Glu Val Asn 
                2565                2570                2575    
Leu Lys Lys Tyr Ser Gly Cys Leu Lys Asp Ile Glu Ile Ser Arg Thr 
            2580                2585                2590        
Pro Tyr Asn Ile Leu Ser Ser Pro Asp Tyr Val Gly Val Thr Lys Gly 
        2595                2600                2605            
Cys Ser Leu Glu Asn Val Tyr Thr Val Ser Phe Pro Lys Pro Gly Phe 
    2610                2615                2620                
Val Glu Leu Ser Pro Val Pro Ile Asp Val Gly Thr Glu Ile Asn Leu 
2625                2630                2635                2640
Ser Phe Ser Thr Lys Asn Glu Ser Gly Ile Ile Leu Leu Gly Ser Gly 
                2645                2650                2655    
Gly Thr Pro Ala Pro Pro Arg Arg Lys Arg Arg Gln Thr Gly Gln Ala 
            2660                2665                2670        
Tyr Tyr Ala Ile Leu Leu Asn Arg Gly Arg Leu Glu Val His Leu Ser 
        2675                2680                2685            
Thr Gly Ala Arg Thr Met Arg Lys Ile Val Ile Arg Pro Glu Pro Asn 
    2690                2695                2700                
Leu Phe His Asp Gly Arg Glu His Ser Val His Val Glu Arg Thr Arg 
2705                2710                2715                2720
Gly Ile Phe Thr Val Gln Val Asp Glu Asn Arg Arg Tyr Met Gln Asn 
                2725                2730                2735    
Leu Thr Val Glu Gln Pro Ile Glu Val Lys Lys Leu Phe Val Gly Gly 
            2740                2745                2750        
Ala Pro Pro Glu Phe Gln Pro Ser Pro Leu Arg Asn Ile Pro Pro Phe 
        2755                2760                2765            
Glu Gly Cys Ile Trp Asn Leu Val Ile Asn Ser Val Pro Met Asp Phe 
    2770                2775                2780                
Ala Arg Pro Val Ser Phe Lys Asn Ala Asp Ile Gly Arg Cys Ala His 
2785                2790                2795                2800
Gln Lys Leu Arg Glu Asp Glu Asp Gly Ala Ala Pro Ala Glu Ile Val 
                2805                2810                2815    
Ile Gln Pro Glu Pro Val Pro Thr Pro Ala Phe Pro Thr Pro Thr Pro 
            2820                2825                2830        
Val Leu Thr His Gly Pro Cys Ala Ala Glu Ser Glu Pro Ala Leu Leu 
        2835                2840                2845            
Ile Gly Ser Lys Gln Phe Gly Leu Ser Arg Asn Ser His Ile Ala Ile 
    2850                2855                2860                
Ala Phe Asp Asp Thr Lys Val Lys Asn Arg Leu Thr Ile Glu Leu Glu 
2865                2870                2875                2880
Val Arg Thr Glu Ala Glu Ser Gly Leu Leu Phe Tyr Met Ala Arg Ile 
                2885                2890                2895    
Asn His Ala Asp Phe Ala Thr Val Gln Leu Arg Asn Gly Leu Pro Tyr 
            2900                2905                2910        
Phe Ser Tyr Asp Leu Gly Ser Gly Asp Thr His Thr Met Ile Pro Thr 
        2915                2920                2925            
Lys Ile Asn Asp Gly Gln Trp His Lys Ile Lys Ile Met Arg Ser Lys 
    2930                2935                2940                
Gln Glu Gly Ile Leu Tyr Val Asp Gly Ala Ser Asn Arg Thr Ile Ser 
2945                2950                2955                2960
Pro Lys Lys Ala Asp Ile Leu Asp Val Val Gly Met Leu Tyr Val Gly 
                2965                2970                2975    
Gly Leu Pro Ile Asn Tyr Thr Thr Arg Arg Ile Gly Pro Val Thr Tyr 
            2980                2985                2990        
Ser Ile Asp Gly Cys Val Arg Asn Leu His Met Ala Glu Ala Pro Ala 
        2995                3000                3005            
Asp Leu Glu Gln Pro Thr Ser Ser Phe His Val Gly Thr Cys Phe Ala 
    3010                3015                3020                
Asn Ala Gln Arg Gly Thr Tyr Phe Asp Gly Thr Gly Phe Ala Lys Ala 
3025                3030                3035                3040
Val Gly Gly Phe Lys Val Gly Leu Asp Leu Leu Val Glu Phe Glu Phe 
                3045                3050                3055    
Arg Thr Thr Thr Thr Thr Gly Val Leu Leu Gly Ile Ser Ser Gln Lys 
            3060                3065                3070        
Met Asp Gly Met Gly Ile Glu Met Ile Asp Glu Lys Leu Met Phe His 
        3075                3080                3085            
Val Asp Asn Gly Ala Gly Arg Phe Thr Ala Val Tyr Asp Ala Gly Val 
    3090                3095                3100                
Pro Gly His Leu Cys Asp Gly Gln Trp His Lys Val Thr Ala Asn Lys 
3105                3110                3115                3120
Ile Lys His Arg Ile Glu Leu Thr Val Asp Gly Asn Gln Val Glu Ala 
                3125                3130                3135    
Gln Ser Pro Asn Pro Ala Ser Thr Ser Ala Asp Thr Asn Asp Pro Val 
            3140                3145                3150        
Phe Val Gly Gly Phe Pro Asp Asp Leu Lys Gln Phe Gly Leu Thr Thr 
        3155                3160                3165            
Ser Ile Pro Phe Arg Gly Cys Ile Arg Ser Leu Lys Leu Thr Lys Gly 
    3170                3175                3180                
Thr Gly Lys Pro Leu Glu Val Asn Phe Ala Lys Ala Leu Glu Leu Arg 
3185                3190                3195                3200
Gly Val Gln Pro Val Ser Cys Pro Ala Asn 
                3205                3210

<210> 117
<211> 3207
<212> PRT
<213> Artificial Sequence


<220> 
<223> laminin subunit α2 isoform X2

<400> 117
Met Pro Gly Ala Ala Gly Val Leu Leu Leu Leu Leu Leu Ser Gly Gly 
1               5                   10                  15      
Leu Gly Gly Val Gln Ala Gln Arg Pro Gln Gln Gln Arg Gln Ser Gln 
            20                  25                  30          
Ala His Gln Gln Arg Gly Leu Phe Pro Ala Val Leu Asn Leu Ala Ser 
        35                  40                  45              
Asn Ala Leu Ile Thr Thr Asn Ala Thr Cys Gly Glu Lys Gly Pro Glu 
    50                  55                  60                  
Met Tyr Cys Lys Leu Val Glu His Val Pro Gly Gln Pro Val Arg Asn 
65                  70                  75                  80  
Pro Gln Cys Arg Ile Cys Asn Gln Asn Ser Ser Asn Pro Asn Gln Arg 
                85                  90                  95      
His Pro Ile Thr Asn Ala Ile Asp Gly Lys Asn Thr Trp Trp Gln Ser 
            100                 105                 110         
Pro Ser Ile Lys Asn Gly Ile Glu Tyr His Tyr Val Thr Ile Thr Leu 
        115                 120                 125             
Asp Leu Gln Gln Val Phe Gln Ile Ala Tyr Val Ile Val Lys Ala Ala 
    130                 135                 140                 
Asn Ser Pro Arg Pro Gly Asn Trp Ile Leu Glu Arg Ser Leu Asp Asp 
145                 150                 155                 160 
Val Glu Tyr Lys Pro Trp Gln Tyr His Ala Val Thr Asp Thr Glu Cys 
                165                 170                 175     
Leu Thr Leu Tyr Asn Ile Tyr Pro Arg Thr Gly Pro Pro Ser Tyr Ala 
            180                 185                 190         
Lys Asp Asp Glu Val Ile Cys Thr Ser Phe Tyr Ser Lys Ile His Pro 
        195                 200                 205             
Leu Glu Asn Gly Glu Ile His Ile Ser Leu Ile Asn Gly Arg Pro Ser 
    210                 215                 220                 
Ala Asp Asp Pro Ser Pro Glu Leu Leu Glu Phe Thr Ser Ala Arg Tyr 
225                 230                 235                 240 
Ile Arg Leu Arg Phe Gln Arg Ile Arg Thr Leu Asn Ala Asp Leu Met 
                245                 250                 255     
Met Phe Ala His Lys Asp Pro Arg Glu Ile Asp Pro Ile Val Thr Arg 
            260                 265                 270         
Arg Tyr Tyr Tyr Ser Val Lys Asp Ile Ser Val Gly Gly Met Cys Ile 
        275                 280                 285             
Cys Tyr Gly His Ala Arg Ala Cys Pro Leu Asp Pro Ala Thr Asn Lys 
    290                 295                 300                 
Ser Arg Cys Glu Cys Glu His Asn Thr Cys Gly Asp Ser Cys Asp Gln 
305                 310                 315                 320 
Cys Cys Pro Gly Phe His Gln Lys Pro Trp Arg Ala Gly Thr Phe Leu 
                325                 330                 335     
Thr Lys Thr Glu Cys Glu Ala Cys Asn Cys His Gly Lys Ala Glu Glu 
            340                 345                 350         
Cys Tyr Tyr Asp Glu Asn Val Ala Arg Arg Asn Leu Ser Leu Asn Ile 
        355                 360                 365             
Arg Gly Lys Tyr Ile Gly Gly Gly Val Cys Ile Asn Cys Thr Gln Asn 
    370                 375                 380                 
Thr Ala Gly Ile Asn Cys Glu Thr Cys Thr Asp Gly Phe Phe Arg Pro 
385                 390                 395                 400 
Lys Gly Val Ser Pro Asn Tyr Pro Arg Pro Cys Gln Pro Cys His Cys 
                405                 410                 415     
Asp Pro Ile Gly Ser Leu Asn Glu Val Cys Val Lys Asp Glu Lys His 
            420                 425                 430         
Ala Arg Arg Gly Leu Ala Pro Gly Ser Cys His Cys Lys Thr Gly Phe 
        435                 440                 445             
Gly Gly Val Ser Cys Asp Arg Cys Ala Arg Gly Tyr Thr Gly Tyr Pro 
    450                 455                 460                 
Asp Cys Lys Ala Cys Asn Cys Ser Gly Leu Gly Ser Lys Asn Glu Asp 
465                 470                 475                 480 
Pro Cys Phe Gly Pro Cys Ile Cys Lys Glu Asn Val Glu Gly Gly Asp 
                485                 490                 495     
Cys Ser Arg Cys Lys Ser Gly Phe Phe Asn Leu Gln Glu Asp Asn Trp 
            500                 505                 510         
Lys Gly Cys Asp Glu Cys Phe Cys Ser Gly Val Ser Asn Arg Cys Gln 
        515                 520                 525             
Ser Ser Tyr Trp Thr Tyr Gly Lys Ile Gln Asp Met Ser Gly Trp Tyr 
    530                 535                 540                 
Leu Thr Asp Leu Pro Gly Arg Ile Arg Val Ala Pro Gln Gln Asp Asp 
545                 550                 555                 560 
Leu Asp Ser Pro Gln Gln Ile Ser Ile Ser Asn Ala Glu Ala Arg Gln 
                565                 570                 575     
Ala Leu Pro His Ser Tyr Tyr Trp Ser Ala Pro Ala Pro Tyr Leu Gly 
            580                 585                 590         
Asn Lys Leu Pro Ala Val Gly Gly Gln Leu Thr Phe Thr Ile Ser Tyr 
        595                 600                 605             
Asp Leu Glu Glu Glu Glu Glu Asp Thr Glu Arg Val Leu Gln Leu Met 
    610                 615                 620                 
Ile Ile Leu Glu Gly Asn Asp Leu Ser Ile Ser Thr Ala Gln Asp Glu 
625                 630                 635                 640 
Val Tyr Leu His Pro Ser Glu Glu His Thr Asn Val Leu Leu Leu Lys 
                645                 650                 655     
Glu Glu Ser Phe Thr Ile His Gly Thr His Phe Pro Val Arg Arg Lys 
            660                 665                 670         
Glu Phe Met Thr Val Leu Ala Asn Leu Lys Arg Val Leu Leu Gln Ile 
        675                 680                 685             
Thr Tyr Ser Phe Gly Met Asp Ala Ile Phe Arg Leu Ser Ser Val Asn 
    690                 695                 700                 
Leu Glu Ser Ala Val Ser Tyr Pro Thr Asp Gly Ser Ile Ala Ala Ala 
705                 710                 715                 720 
Val Glu Val Cys Gln Cys Pro Pro Gly Tyr Thr Gly Ser Ser Cys Glu 
                725                 730                 735     
Ser Cys Trp Pro Arg His Arg Arg Val Asn Gly Thr Ile Phe Gly Gly 
            740                 745                 750         
Ile Cys Glu Pro Cys Gln Cys Phe Gly His Ala Glu Ser Cys Asp Asp 
        755                 760                 765             
Val Thr Gly Glu Cys Leu Asn Cys Lys Asp His Thr Gly Gly Pro Tyr 
    770                 775                 780                 
Cys Asp Lys Cys Leu Pro Gly Phe Tyr Gly Glu Pro Thr Lys Gly Thr 
785                 790                 795                 800 
Ser Glu Asp Cys Gln Pro Cys Ala Cys Pro Leu Asn Ile Pro Ser Asn 
                805                 810                 815     
Asn Phe Ser Pro Thr Cys His Leu Asp Arg Ser Leu Gly Leu Ile Cys 
            820                 825                 830         
Asp Gly Cys Pro Val Gly Tyr Thr Gly Pro Arg Cys Glu Arg Cys Ala 
        835                 840                 845             
Glu Gly Tyr Phe Gly Gln Pro Ser Val Pro Gly Gly Ser Cys Gln Pro 
    850                 855                 860                 
Cys Gln Cys Asn Asp Asn Leu Asp Phe Ser Ile Pro Gly Ser Cys Asp 
865                 870                 875                 880 
Ser Leu Ser Gly Ser Cys Leu Ile Cys Lys Pro Gly Thr Thr Gly Arg 
                885                 890                 895     
Tyr Cys Glu Leu Cys Ala Asp Gly Tyr Phe Gly Asp Ala Val Asp Ala 
            900                 905                 910         
Lys Asn Cys Gln Pro Cys Arg Cys Asn Ala Gly Gly Ser Phe Ser Glu 
        915                 920                 925             
Val Cys His Ser Gln Thr Gly Gln Cys Glu Cys Arg Ala Asn Val Gln 
    930                 935                 940                 
Gly Gln Arg Cys Asp Lys Cys Lys Pro Asn Met Trp Arg Asp Pro Glu 
945                 950                 955                 960 
Lys Arg Phe Cys Val Leu Cys Asp Cys Asp Pro Val Gly Ser Val Ser 
                965                 970                 975     
Pro Gln Cys Asp Ile Thr Gly Arg Cys Val Cys Lys Ser Gly Phe Val 
            980                 985                 990         
Gly Lys Gln Cys Asn Leu Gly Arg Gln Val His Gln Gln Glu Glu Gln 
        995                 1000                1005            
Pro Arg Arg Ala Gln Arg Val Leu Gly Ser Pro Gln Arg Trp Ala Ile 
    1010                1015                1020                
Gly Ser Ser Ser Gly Cys Pro Arg Gly Ala Tyr Arg Ala Pro Ala Pro 
1025                1030                1035                1040
Ala Gly Thr Phe Gly Leu Gln Ser Ala Arg Gly Cys Val Pro Cys Asn 
                1045                1050                1055    
Cys Asn Ser Phe Gly Ser Lys Ser Phe Asp Cys Glu Glu Ser Gly Gln 
            1060                1065                1070        
Cys Trp Cys Gln Pro Gly Val Thr Gly Lys Lys Cys Asp Arg Cys Ala 
        1075                1080                1085            
His Gly Tyr Phe Asn Phe Gln Glu Gly Gly Cys Thr Ala Cys Glu Cys 
    1090                1095                1100                
Ser His Leu Gly Asn Asn Cys Asp Pro Lys Thr Gly Arg Cys Ile Cys 
1105                1110                1115                1120
Pro Pro Asn Thr Ile Gly Glu Lys Cys Ser Lys Cys Ala Pro Asn Thr 
                1125                1130                1135    
Trp Gly His Ser Ile Thr Thr Gly Cys Lys Ala Cys Asn Cys Ser Thr 
            1140                1145                1150        
Val Gly Ser Leu Asp Phe Gln Cys Asn Val Asn Thr Gly Gln Cys Asn 
        1155                1160                1165            
Cys His Pro Lys Phe Ser Gly Ala Lys Cys Thr Glu Cys Ser Arg Gly 
    1170                1175                1180                
His Trp Asn Tyr Pro Arg Cys Asn Leu Cys Asp Cys Phe Leu Pro Gly 
1185                1190                1195                1200
Thr Asp Ala Thr Thr Cys Asp Ser Glu Thr Lys Lys Cys Ser Cys Ser 
                1205                1210                1215    
Asp Gln Thr Gly Gln Cys Thr Cys Lys Val Asn Val Glu Gly Ile His 
            1220                1225                1230        
Cys Asp Arg Cys Arg Pro Gly Lys Phe Gly Leu Asp Ala Lys Asn Pro 
        1235                1240                1245            
Leu Gly Cys Ser Ser Cys Tyr Cys Phe Gly Thr Thr Thr Gln Cys Ser 
    1250                1255                1260                
Glu Ala Lys Gly Leu Ile Arg Thr Trp Val Thr Leu Lys Ala Glu Gln 
1265                1270                1275                1280
Thr Ile Leu Pro Leu Val Asp Glu Ala Leu Gln His Thr Thr Thr Lys 
                1285                1290                1295    
Gly Ile Val Phe Gln His Pro Glu Ile Val Ala His Met Asp Leu Met 
            1300                1305                1310        
Arg Glu Asp Leu His Leu Glu Pro Phe Tyr Trp Lys Leu Pro Glu Gln 
        1315                1320                1325            
Phe Glu Gly Lys Lys Leu Met Ala Tyr Gly Gly Lys Leu Lys Tyr Ala 
    1330                1335                1340                
Ile Tyr Phe Glu Ala Arg Glu Glu Thr Gly Phe Ser Thr Tyr Asn Pro 
1345                1350                1355                1360
Gln Val Ile Ile Arg Gly Gly Thr Pro Thr His Ala Arg Ile Ile Val 
                1365                1370                1375    
Arg His Met Ala Ala Pro Leu Ile Gly Gln Leu Thr Arg His Glu Ile 
            1380                1385                1390        
Glu Met Thr Glu Lys Glu Trp Lys Tyr Tyr Gly Asp Asp Pro Arg Val 
        1395                1400                1405            
His Arg Thr Val Thr Arg Glu Asp Phe Leu Asp Ile Leu Tyr Asp Ile 
    1410                1415                1420                
His Tyr Ile Leu Ile Lys Ala Thr Tyr Gly Asn Phe Met Arg Gln Ser 
1425                1430                1435                1440
Arg Ile Ser Glu Ile Ser Met Glu Val Ala Glu Gln Gly Arg Gly Thr 
                1445                1450                1455    
Thr Met Thr Pro Pro Ala Asp Leu Ile Glu Lys Cys Asp Cys Pro Leu 
            1460                1465                1470        
Gly Tyr Ser Gly Leu Ser Cys Glu Ala Cys Leu Pro Gly Phe Tyr Arg 
        1475                1480                1485            
Leu Arg Ser Gln Pro Gly Gly Arg Thr Pro Gly Pro Thr Leu Gly Thr 
    1490                1495                1500                
Cys Val Pro Cys Gln Cys Asn Gly His Ser Ser Leu Cys Asp Pro Glu 
1505                1510                1515                1520
Thr Ser Ile Cys Gln Asn Cys Gln His His Thr Ala Gly Asp Phe Cys 
                1525                1530                1535    
Glu Arg Cys Ala Leu Gly Tyr Tyr Gly Ile Val Lys Gly Leu Pro Asn 
            1540                1545                1550        
Asp Cys Gln Gln Cys Ala Cys Pro Leu Ile Ser Ser Ser Asn Asn Phe 
        1555                1560                1565            
Ser Pro Ser Cys Val Ala Glu Gly Leu Asp Asp Tyr Arg Cys Thr Ala 
    1570                1575                1580                
Cys Pro Arg Gly Tyr Glu Gly Gln Tyr Cys Glu Arg Cys Ala Pro Gly 
1585                1590                1595                1600
Tyr Thr Gly Ser Pro Gly Asn Pro Gly Ser Cys Gln Glu Cys Glu Cys 
                1605                1610                1615    
Asp Pro Tyr Gly Ser Leu Pro Val Pro Cys Asp Pro Val Thr Gly Phe 
            1620                1625                1630        
Cys Thr Cys Arg Pro Gly Ala Thr Gly Arg Lys Cys Asp Gly Cys Lys 
        1635                1640                1645            
His Trp His Ala Arg Glu Gly Trp Glu Cys Val Phe Cys Gly Asp Glu 
    1650                1655                1660                
Cys Thr Gly Leu Leu Leu Gly Asp Leu Ala Arg Leu Glu Gln Met Val 
1665                1670                1675                1680
Met Ser Ile Asn Leu Thr Gly Pro Leu Pro Ala Pro Tyr Lys Met Leu 
                1685                1690                1695    
Tyr Gly Leu Glu Asn Met Thr Gln Glu Leu Lys His Leu Leu Ser Pro 
            1700                1705                1710        
Gln Arg Ala Pro Glu Arg Leu Ile Gln Leu Ala Glu Gly Asn Leu Asn 
        1715                1720                1725            
Thr Leu Val Thr Glu Met Asn Glu Leu Leu Thr Arg Ala Thr Lys Val 
    1730                1735                1740                
Thr Ala Asp Gly Glu Gln Thr Gly Gln Asp Ala Glu Arg Thr Asn Thr 
1745                1750                1755                1760
Arg Ala Lys Ser Leu Gly Glu Phe Ile Lys Glu Leu Ala Arg Asp Ala 
                1765                1770                1775    
Glu Ala Val Asn Glu Lys Ala Ile Lys Leu Asn Glu Thr Leu Gly Thr 
            1780                1785                1790        
Arg Asp Glu Ala Phe Glu Arg Asn Leu Glu Gly Leu Gln Lys Glu Ile 
        1795                1800                1805            
Asp Gln Met Ile Lys Glu Leu Arg Arg Lys Asn Leu Glu Thr Gln Lys 
    1810                1815                1820                
Glu Ile Ala Glu Asp Glu Leu Val Ala Ala Glu Ala Leu Leu Lys Lys 
1825                1830                1835                1840
Val Lys Lys Leu Phe Gly Glu Ser Arg Gly Glu Asn Glu Glu Met Glu 
                1845                1850                1855    
Lys Asp Leu Arg Glu Lys Leu Ala Asp Tyr Lys Asn Lys Val Asp Asp 
            1860                1865                1870        
Ala Trp Asp Leu Leu Arg Glu Ala Thr Asp Lys Ile Arg Glu Ala Asn 
        1875                1880                1885            
Arg Leu Phe Ala Val Asn Gln Lys Asn Met Thr Ala Leu Glu Lys Lys 
    1890                1895                1900                
Lys Glu Ala Val Glu Ser Gly Lys Arg Gln Ile Glu Asn Thr Leu Lys 
1905                1910                1915                1920
Glu Gly Asn Asp Ile Leu Asp Glu Ala Asn Arg Leu Ala Asp Glu Ile 
                1925                1930                1935    
Asn Ser Ile Ile Asp Tyr Val Glu Asp Ile Gln Thr Lys Leu Pro Pro 
            1940                1945                1950        
Met Ser Glu Glu Leu Asn Asp Lys Ile Asp Asp Leu Ser Gln Glu Ile 
        1955                1960                1965            
Lys Asp Arg Lys Leu Ala Glu Lys Val Ser Gln Ala Glu Ser His Ala 
    1970                1975                1980                
Ala Gln Leu Asn Asp Ser Ser Ala Val Leu Asp Gly Ile Leu Asp Glu 
1985                1990                1995                2000
Ala Lys Asn Ile Ser Phe Asn Ala Thr Ala Ala Phe Lys Ala Tyr Ser 
                2005                2010                2015    
Asn Ile Lys Asp Tyr Ile Asp Glu Ala Glu Lys Val Ala Lys Glu Ala 
            2020                2025                2030        
Lys Asp Leu Ala His Glu Ala Thr Lys Leu Ala Thr Gly Pro Arg Gly 
        2035                2040                2045            
Leu Leu Lys Glu Asp Ala Lys Gly Cys Leu Gln Lys Ser Phe Arg Ile 
    2050                2055                2060                
Leu Asn Glu Ala Lys Lys Leu Ala Asn Asp Val Lys Glu Asn Glu Asp 
2065                2070                2075                2080
His Leu Asn Gly Leu Lys Thr Arg Ile Glu Asn Ala Asp Ala Arg Asn 
                2085                2090                2095    
Gly Asp Leu Leu Arg Thr Leu Asn Asp Thr Leu Gly Lys Leu Ser Ala 
            2100                2105                2110        
Ile Pro Asn Asp Thr Ala Ala Lys Leu Gln Ala Val Lys Asp Lys Ala 
        2115                2120                2125            
Arg Gln Ala Asn Asp Thr Ala Lys Asp Val Leu Ala Gln Ile Thr Glu 
    2130                2135                2140                
Leu His Gln Asn Leu Asp Gly Leu Lys Lys Asn Tyr Asn Lys Leu Ala 
2145                2150                2155                2160
Asp Ser Val Ala Lys Thr Asn Ala Val Val Lys Asp Pro Ser Lys Asn 
                2165                2170                2175    
Ile Ala Asp Ala Asp Ala Thr Val Lys Asn Leu Glu Gln Glu Ala Asp 
            2180                2185                2190        
Arg Leu Ile Asp Lys Leu Lys Pro Ile Lys Glu Leu Glu Asp Asn Leu 
        2195                2200                2205            
Lys Lys Asn Ile Ser Glu Ile Lys Glu Leu Ile Asn Gln Ala Arg Lys 
    2210                2215                2220                
Gln Ala Asn Ser Ile Lys Val Ser Val Ser Ser Gly Gly Asp Cys Ile 
2225                2230                2235                2240
Arg Thr Tyr Lys Pro Glu Ile Lys Lys Gly Ser Tyr Asn Asn Ile Val 
                2245                2250                2255    
Val Asn Val Lys Thr Ala Val Ala Asp Asn Leu Leu Phe Tyr Leu Gly 
            2260                2265                2270        
Ser Ala Lys Phe Ile Asp Phe Leu Ala Ile Glu Met Arg Lys Gly Lys 
        2275                2280                2285            
Val Ser Phe Leu Trp Asp Val Gly Ser Gly Val Gly Arg Val Glu Tyr 
    2290                2295                2300                
Pro Asp Leu Thr Ile Asp Asp Ser Tyr Trp Tyr Arg Ile Val Ala Ser 
2305                2310                2315                2320
Arg Thr Gly Arg Asn Gly Thr Ile Ser Val Arg Ala Leu Asp Gly Pro 
                2325                2330                2335    
Lys Ala Ser Ile Val Pro Ser Thr His His Ser Thr Ser Pro Pro Gly 
            2340                2345                2350        
Tyr Thr Ile Leu Asp Val Asp Ala Asn Ala Met Leu Phe Val Gly Gly 
        2355                2360                2365            
Leu Thr Gly Lys Leu Lys Lys Ala Asp Ala Val Arg Val Ile Thr Phe 
    2370                2375                2380                
Thr Gly Cys Met Gly Glu Thr Tyr Phe Asp Asn Lys Pro Ile Gly Leu 
2385                2390                2395                2400
Trp Asn Phe Arg Glu Lys Glu Gly Asp Cys Lys Gly Cys Thr Val Ser 
                2405                2410                2415    
Pro Gln Val Glu Asp Ser Glu Gly Thr Ile Gln Phe Asp Gly Glu Gly 
            2420                2425                2430        
Tyr Ala Leu Val Ser Arg Pro Ile Arg Trp Tyr Pro Asn Ile Ser Thr 
        2435                2440                2445            
Val Met Phe Lys Phe Arg Thr Phe Ser Ser Ser Ala Leu Leu Met Tyr 
    2450                2455                2460                
Leu Ala Thr Arg Asp Leu Arg Asp Phe Met Ser Val Glu Leu Thr Asp 
2465                2470                2475                2480
Gly His Ile Lys Val Ser Tyr Asp Leu Gly Ser Gly Met Ala Ser Val 
                2485                2490                2495    
Val Ser Asn Gln Asn His Asn Asp Gly Lys Trp Lys Ser Phe Thr Leu 
            2500                2505                2510        
Ser Arg Ile Gln Lys Gln Ala Asn Ile Ser Ile Val Asp Ile Asp Thr 
        2515                2520                2525            
Asn Gln Glu Glu Asn Ile Ala Thr Ser Ser Ser Gly Asn Asn Phe Gly 
    2530                2535                2540                
Leu Asp Leu Lys Ala Asp Asp Lys Ile Tyr Phe Gly Gly Leu Pro Thr 
2545                2550                2555                2560
Leu Arg Asn Leu Ser Met Lys Ala Arg Pro Glu Val Asn Leu Lys Lys 
                2565                2570                2575    
Tyr Ser Gly Cys Leu Lys Asp Ile Glu Ile Ser Arg Thr Pro Tyr Asn 
            2580                2585                2590        
Ile Leu Ser Ser Pro Asp Tyr Val Gly Val Thr Lys Gly Cys Ser Leu 
        2595                2600                2605            
Glu Asn Val Tyr Thr Val Ser Phe Pro Lys Pro Gly Phe Val Glu Leu 
    2610                2615                2620                
Ser Pro Val Pro Ile Asp Val Gly Thr Glu Ile Asn Leu Ser Phe Ser 
2625                2630                2635                2640
Thr Lys Asn Glu Ser Gly Ile Ile Leu Leu Gly Ser Gly Gly Thr Pro 
                2645                2650                2655    
Ala Pro Pro Arg Arg Lys Arg Arg Gln Thr Gly Gln Ala Tyr Tyr Ala 
            2660                2665                2670        
Ile Leu Leu Asn Arg Gly Arg Leu Glu Val His Leu Ser Thr Gly Ala 
        2675                2680                2685            
Arg Thr Met Arg Lys Ile Val Ile Arg Pro Glu Pro Asn Leu Phe His 
    2690                2695                2700                
Asp Gly Arg Glu His Ser Val His Val Glu Arg Thr Arg Gly Ile Phe 
2705                2710                2715                2720
Thr Val Gln Val Asp Glu Asn Arg Arg Tyr Met Gln Asn Leu Thr Val 
                2725                2730                2735    
Glu Gln Pro Ile Glu Val Lys Lys Leu Phe Val Gly Gly Ala Pro Pro 
            2740                2745                2750        
Glu Phe Gln Pro Ser Pro Leu Arg Asn Ile Pro Pro Phe Glu Gly Cys 
        2755                2760                2765            
Ile Trp Asn Leu Val Ile Asn Ser Val Pro Met Asp Phe Ala Arg Pro 
    2770                2775                2780                
Val Ser Phe Lys Asn Ala Asp Ile Gly Arg Cys Ala His Gln Lys Leu 
2785                2790                2795                2800
Arg Glu Asp Glu Asp Gly Ala Ala Pro Ala Glu Ile Val Ile Gln Pro 
                2805                2810                2815    
Glu Pro Val Pro Thr Pro Ala Phe Pro Thr Pro Thr Pro Val Leu Thr 
            2820                2825                2830        
His Gly Pro Cys Ala Ala Glu Ser Glu Pro Ala Leu Leu Ile Gly Ser 
        2835                2840                2845            
Lys Gln Phe Gly Leu Ser Arg Asn Ser His Ile Ala Ile Ala Phe Asp 
    2850                2855                2860                
Asp Thr Lys Val Lys Asn Arg Leu Thr Ile Glu Leu Glu Val Arg Thr 
2865                2870                2875                2880
Glu Ala Glu Ser Gly Leu Leu Phe Tyr Met Ala Arg Ile Asn His Ala 
                2885                2890                2895    
Asp Phe Ala Thr Val Gln Leu Arg Asn Gly Leu Pro Tyr Phe Ser Tyr 
            2900                2905                2910        
Asp Leu Gly Ser Gly Asp Thr His Thr Met Ile Pro Thr Lys Ile Asn 
        2915                2920                2925            
Asp Gly Gln Trp His Lys Ile Lys Ile Met Arg Ser Lys Gln Glu Gly 
    2930                2935                2940                
Ile Leu Tyr Val Asp Gly Ala Ser Asn Arg Thr Ile Ser Pro Lys Lys 
2945                2950                2955                2960
Ala Asp Ile Leu Asp Val Val Gly Met Leu Tyr Val Gly Gly Leu Pro 
                2965                2970                2975    
Ile Asn Tyr Thr Thr Arg Arg Ile Gly Pro Val Thr Tyr Ser Ile Asp 
            2980                2985                2990        
Gly Cys Val Arg Asn Leu His Met Ala Glu Ala Pro Ala Asp Leu Glu 
        2995                3000                3005            
Gln Pro Thr Ser Ser Phe His Val Gly Thr Cys Phe Ala Asn Ala Gln 
    3010                3015                3020                
Arg Gly Thr Tyr Phe Asp Gly Thr Gly Phe Ala Lys Ala Val Gly Gly 
3025                3030                3035                3040
Phe Lys Val Gly Leu Asp Leu Leu Val Glu Phe Glu Phe Arg Thr Thr 
                3045                3050                3055    
Thr Thr Thr Gly Val Leu Leu Gly Ile Ser Ser Gln Lys Met Asp Gly 
            3060                3065                3070        
Met Gly Ile Glu Met Ile Asp Glu Lys Leu Met Phe His Val Asp Asn 
        3075                3080                3085            
Gly Ala Gly Arg Phe Thr Ala Val Tyr Asp Ala Gly Val Pro Gly His 
    3090                3095                3100                
Leu Cys Asp Gly Gln Trp His Lys Val Thr Ala Asn Lys Ile Lys His 
3105                3110                3115                3120
Arg Ile Glu Leu Thr Val Asp Gly Asn Gln Val Glu Ala Gln Ser Pro 
                3125                3130                3135    
Asn Pro Ala Ser Thr Ser Ala Asp Thr Asn Asp Pro Val Phe Val Gly 
            3140                3145                3150        
Gly Phe Pro Asp Asp Leu Lys Gln Phe Gly Leu Thr Thr Ser Ile Pro 
        3155                3160                3165            
Phe Arg Gly Cys Ile Arg Ser Leu Lys Leu Thr Lys Gly Thr Gly Lys 
    3170                3175                3180                
Pro Leu Glu Val Asn Phe Ala Lys Ala Leu Glu Leu Arg Gly Val Gln 
3185                3190                3195                3200
Pro Val Ser Cys Pro Ala Asn 
                3205        

<210> 118
<211> 3206
<212> PRT
<213> Artificial Sequence


<220> 
<223> laminin subunit α2 isoform X3

<400> 118
Met Pro Gly Ala Ala Gly Val Leu Leu Leu Leu Leu Leu Ser Gly Gly 
1               5                   10                  15      
Leu Gly Gly Val Gln Ala Gln Arg Pro Gln Gln Gln Arg Gln Ser Gln 
            20                  25                  30          
Ala His Gln Gln Arg Gly Leu Phe Pro Ala Val Leu Asn Leu Ala Ser 
        35                  40                  45              
Asn Ala Leu Ile Thr Thr Asn Ala Thr Cys Gly Glu Lys Gly Pro Glu 
    50                  55                  60                  
Met Tyr Cys Lys Leu Val Glu His Val Pro Gly Gln Pro Val Arg Asn 
65                  70                  75                  80  
Pro Gln Cys Arg Ile Cys Asn Gln Asn Ser Ser Asn Pro Asn Gln Arg 
                85                  90                  95      
His Pro Ile Thr Asn Ala Ile Asp Gly Lys Asn Thr Trp Trp Gln Ser 
            100                 105                 110         
Pro Ser Ile Lys Asn Gly Ile Glu Tyr His Tyr Val Thr Ile Thr Leu 
        115                 120                 125             
Asp Leu Gln Gln Val Phe Gln Ile Ala Tyr Val Ile Val Lys Ala Ala 
    130                 135                 140                 
Asn Ser Pro Arg Pro Gly Asn Trp Ile Leu Glu Arg Ser Leu Asp Asp 
145                 150                 155                 160 
Val Glu Tyr Lys Pro Trp Gln Tyr His Ala Val Thr Asp Thr Glu Cys 
                165                 170                 175     
Leu Thr Leu Tyr Asn Ile Tyr Pro Arg Thr Gly Pro Pro Ser Tyr Ala 
            180                 185                 190         
Lys Asp Asp Glu Val Ile Cys Thr Ser Phe Tyr Ser Lys Ile His Pro 
        195                 200                 205             
Leu Glu Asn Gly Glu Ile His Ile Ser Leu Ile Asn Gly Arg Pro Ser 
    210                 215                 220                 
Ala Asp Asp Pro Ser Pro Glu Leu Leu Glu Phe Thr Ser Ala Arg Tyr 
225                 230                 235                 240 
Ile Arg Leu Arg Phe Gln Arg Ile Arg Thr Leu Asn Ala Asp Leu Met 
                245                 250                 255     
Met Phe Ala His Lys Asp Pro Arg Glu Ile Asp Pro Ile Val Thr Arg 
            260                 265                 270         
Arg Tyr Tyr Tyr Ser Val Lys Asp Ile Ser Val Gly Gly Met Cys Ile 
        275                 280                 285             
Cys Tyr Gly His Ala Arg Ala Cys Pro Leu Asp Pro Ala Thr Asn Lys 
    290                 295                 300                 
Ser Arg Cys Glu Cys Glu His Asn Thr Cys Gly Asp Ser Cys Asp Gln 
305                 310                 315                 320 
Cys Cys Pro Gly Phe His Gln Lys Pro Trp Arg Ala Gly Thr Phe Leu 
                325                 330                 335     
Thr Lys Thr Glu Cys Glu Ala Cys Asn Cys His Gly Lys Ala Glu Glu 
            340                 345                 350         
Cys Tyr Tyr Asp Glu Asn Val Ala Arg Arg Asn Leu Ser Leu Asn Ile 
        355                 360                 365             
Arg Gly Lys Tyr Ile Gly Gly Gly Val Cys Ile Asn Cys Thr Gln Asn 
    370                 375                 380                 
Thr Ala Gly Ile Asn Cys Glu Thr Cys Thr Asp Gly Phe Phe Arg Pro 
385                 390                 395                 400 
Lys Gly Val Ser Pro Asn Tyr Pro Arg Pro Cys Gln Pro Cys His Cys 
                405                 410                 415     
Asp Pro Ile Gly Ser Leu Asn Glu Val Cys Val Lys Asp Glu Lys His 
            420                 425                 430         
Ala Arg Arg Gly Leu Ala Pro Gly Ser Cys His Cys Lys Thr Gly Phe 
        435                 440                 445             
Gly Gly Val Ser Cys Asp Arg Cys Ala Arg Gly Tyr Thr Gly Tyr Pro 
    450                 455                 460                 
Asp Cys Lys Ala Cys Asn Cys Ser Gly Leu Gly Ser Lys Asn Glu Asp 
465                 470                 475                 480 
Pro Cys Phe Gly Pro Cys Ile Cys Lys Glu Asn Val Glu Gly Gly Asp 
                485                 490                 495     
Cys Ser Arg Cys Lys Ser Gly Phe Phe Asn Leu Gln Glu Asp Asn Trp 
            500                 505                 510         
Lys Gly Cys Asp Glu Cys Phe Cys Ser Gly Val Ser Asn Arg Cys Gln 
        515                 520                 525             
Ser Ser Tyr Trp Thr Tyr Gly Lys Ile Gln Asp Met Ser Gly Trp Tyr 
    530                 535                 540                 
Leu Thr Asp Leu Pro Gly Arg Ile Arg Val Ala Pro Gln Gln Asp Asp 
545                 550                 555                 560 
Leu Asp Ser Pro Gln Gln Ile Ser Ile Ser Asn Ala Glu Ala Arg Gln 
                565                 570                 575     
Ala Leu Pro His Ser Tyr Tyr Trp Ser Ala Pro Ala Pro Tyr Leu Gly 
            580                 585                 590         
Asn Lys Leu Pro Ala Val Gly Gly Gln Leu Thr Phe Thr Ile Ser Tyr 
        595                 600                 605             
Asp Leu Glu Glu Glu Glu Glu Asp Thr Glu Arg Val Leu Gln Leu Met 
    610                 615                 620                 
Ile Ile Leu Glu Gly Asn Asp Leu Ser Ile Ser Thr Ala Gln Asp Glu 
625                 630                 635                 640 
Val Tyr Leu His Pro Ser Glu Glu His Thr Asn Val Leu Leu Leu Lys 
                645                 650                 655     
Glu Glu Ser Phe Thr Ile His Gly Thr His Phe Pro Val Arg Arg Lys 
            660                 665                 670         
Glu Phe Met Thr Val Leu Ala Asn Leu Lys Arg Val Leu Leu Gln Ile 
        675                 680                 685             
Thr Tyr Ser Phe Gly Met Asp Ala Ile Phe Arg Leu Ser Ser Val Asn 
    690                 695                 700                 
Leu Glu Ser Ala Val Ser Tyr Pro Thr Asp Gly Ser Ile Ala Ala Ala 
705                 710                 715                 720 
Val Glu Val Cys Gln Cys Pro Pro Gly Tyr Thr Gly Ser Ser Cys Glu 
                725                 730                 735     
Ser Cys Trp Pro Arg His Arg Arg Val Asn Gly Thr Ile Phe Gly Gly 
            740                 745                 750         
Ile Cys Glu Pro Cys Gln Cys Phe Gly His Ala Glu Ser Cys Asp Asp 
        755                 760                 765             
Val Thr Gly Glu Cys Leu Asn Cys Lys Asp His Thr Gly Gly Pro Tyr 
    770                 775                 780                 
Cys Asp Lys Cys Leu Pro Gly Phe Tyr Gly Glu Pro Thr Lys Gly Thr 
785                 790                 795                 800 
Ser Glu Asp Cys Gln Pro Cys Ala Cys Pro Leu Asn Ile Pro Ser Asn 
                805                 810                 815     
Asn Phe Ser Pro Thr Cys His Leu Asp Arg Ser Leu Gly Leu Ile Cys 
            820                 825                 830         
Asp Gly Cys Pro Val Gly Tyr Thr Gly Pro Arg Cys Glu Arg Cys Ala 
        835                 840                 845             
Glu Gly Tyr Phe Gly Gln Pro Ser Val Pro Gly Gly Ser Cys Gln Pro 
    850                 855                 860                 
Cys Gln Cys Asn Asp Asn Leu Asp Phe Ser Ile Pro Gly Ser Cys Asp 
865                 870                 875                 880 
Ser Leu Ser Gly Ser Cys Leu Ile Cys Lys Pro Gly Thr Thr Gly Arg 
                885                 890                 895     
Tyr Cys Glu Leu Cys Ala Asp Gly Tyr Phe Gly Asp Ala Val Asp Ala 
            900                 905                 910         
Lys Asn Cys Gln Pro Cys Arg Cys Asn Ala Gly Gly Ser Phe Ser Glu 
        915                 920                 925             
Val Cys His Ser Gln Thr Gly Gln Cys Glu Cys Arg Ala Asn Val Gln 
    930                 935                 940                 
Gly Gln Arg Cys Asp Lys Cys Lys Pro Asn Met Trp Arg Asp Pro Glu 
945                 950                 955                 960 
Lys Arg Phe Cys Val Leu Cys Asp Cys Asp Pro Val Gly Ser Val Ser 
                965                 970                 975     
Pro Gln Cys Asp Ile Thr Gly Arg Cys Val Cys Lys Ser Gly Phe Val 
            980                 985                 990         
Gly Lys Gln Cys Asn Leu Gly Arg Gln Val His Gln Gln Glu Glu Gln 
        995                 1000                1005            
Pro Arg Arg Ala Gln Arg Val Leu Gly Ser Pro Gln Arg Trp Ala Ile 
    1010                1015                1020                
Gly Ser Ser Ser Gly Cys Pro Arg Gly Ala Tyr Arg Ala Pro Ala Pro 
1025                1030                1035                1040
Ala Gly Thr Phe Gly Leu Gln Ser Ala Arg Gly Cys Val Pro Cys Asn 
                1045                1050                1055    
Cys Asn Ser Phe Gly Ser Lys Ser Phe Asp Cys Glu Glu Ser Gly Gln 
            1060                1065                1070        
Cys Trp Cys Gln Pro Gly Val Thr Gly Lys Lys Cys Asp Arg Cys Ala 
        1075                1080                1085            
His Gly Tyr Phe Asn Phe Gln Glu Gly Gly Cys Thr Ala Cys Glu Cys 
    1090                1095                1100                
Ser His Leu Gly Asn Asn Cys Asp Pro Lys Thr Gly Arg Cys Ile Cys 
1105                1110                1115                1120
Pro Pro Asn Thr Ile Gly Glu Lys Cys Ser Lys Cys Ala Pro Asn Thr 
                1125                1130                1135    
Trp Gly His Ser Ile Thr Thr Gly Cys Lys Ala Cys Asn Cys Ser Thr 
            1140                1145                1150        
Val Gly Ser Leu Asp Phe Gln Cys Asn Val Asn Thr Gly Gln Cys Asn 
        1155                1160                1165            
Cys His Pro Lys Phe Ser Gly Ala Lys Cys Thr Glu Cys Ser Arg Gly 
    1170                1175                1180                
His Trp Asn Tyr Pro Arg Cys Asn Leu Cys Asp Cys Phe Leu Pro Gly 
1185                1190                1195                1200
Thr Asp Ala Thr Thr Cys Asp Ser Glu Thr Lys Lys Cys Ser Cys Ser 
                1205                1210                1215    
Asp Gln Thr Gly Gln Cys Thr Cys Lys Val Asn Val Glu Gly Ile His 
            1220                1225                1230        
Cys Asp Arg Cys Arg Pro Gly Lys Phe Gly Leu Asp Ala Lys Asn Pro 
        1235                1240                1245            
Leu Gly Cys Ser Ser Cys Tyr Cys Phe Gly Thr Thr Thr Gln Cys Ser 
    1250                1255                1260                
Glu Ala Lys Gly Leu Ile Arg Thr Trp Val Thr Leu Lys Ala Glu Gln 
1265                1270                1275                1280
Thr Ile Leu Pro Leu Val Asp Glu Ala Leu Gln His Thr Thr Thr Lys 
                1285                1290                1295    
Gly Ile Val Phe Gln His Pro Glu Ile Val Ala His Met Asp Leu Met 
            1300                1305                1310        
Arg Glu Asp Leu His Leu Glu Pro Phe Tyr Trp Lys Leu Pro Glu Gln 
        1315                1320                1325            
Phe Glu Gly Lys Lys Leu Met Ala Tyr Gly Gly Lys Leu Lys Tyr Ala 
    1330                1335                1340                
Ile Tyr Phe Glu Ala Arg Glu Glu Thr Gly Phe Ser Thr Tyr Asn Pro 
1345                1350                1355                1360
Gln Val Ile Ile Arg Gly Gly Thr Pro Thr His Ala Arg Ile Ile Val 
                1365                1370                1375    
Arg His Met Ala Ala Pro Leu Ile Gly Gln Leu Thr Arg His Glu Ile 
            1380                1385                1390        
Glu Met Thr Glu Lys Glu Trp Lys Tyr Tyr Gly Asp Asp Pro Arg Val 
        1395                1400                1405            
His Arg Thr Val Thr Arg Glu Asp Phe Leu Asp Ile Leu Tyr Asp Ile 
    1410                1415                1420                
His Tyr Ile Leu Ile Lys Ala Thr Tyr Gly Asn Phe Met Arg Gln Ser 
1425                1430                1435                1440
Arg Ile Ser Glu Ile Ser Met Glu Val Ala Glu Gln Gly Arg Gly Thr 
                1445                1450                1455    
Thr Met Thr Pro Pro Ala Asp Leu Ile Glu Lys Cys Asp Cys Pro Leu 
            1460                1465                1470        
Gly Tyr Ser Gly Leu Ser Cys Glu Ala Cys Leu Pro Gly Phe Tyr Arg 
        1475                1480                1485            
Leu Arg Ser Gln Pro Gly Gly Arg Thr Pro Gly Pro Thr Leu Gly Thr 
    1490                1495                1500                
Cys Val Pro Cys Gln Cys Asn Gly His Ser Ser Leu Cys Asp Pro Glu 
1505                1510                1515                1520
Thr Ser Ile Cys Gln Asn Cys Gln His His Thr Ala Gly Asp Phe Cys 
                1525                1530                1535    
Glu Arg Cys Ala Leu Gly Tyr Tyr Gly Ile Val Lys Gly Leu Pro Asn 
            1540                1545                1550        
Asp Cys Gln Gln Cys Ala Cys Pro Leu Ile Ser Ser Ser Asn Asn Phe 
        1555                1560                1565            
Ser Pro Ser Cys Val Ala Glu Gly Leu Asp Asp Tyr Arg Cys Thr Ala 
    1570                1575                1580                
Cys Pro Arg Gly Tyr Glu Gly Gln Tyr Cys Glu Arg Cys Ala Pro Gly 
1585                1590                1595                1600
Tyr Thr Gly Ser Pro Gly Asn Pro Gly Gly Ser Cys Gln Glu Cys Glu 
                1605                1610                1615    
Cys Asp Pro Tyr Gly Ser Leu Pro Val Pro Cys Asp Pro Val Thr Gly 
            1620                1625                1630        
Phe Cys Thr Cys Arg Pro Gly Ala Thr Gly Arg Lys Cys Asp Gly Cys 
        1635                1640                1645            
Lys His Trp His Ala Arg Glu Gly Trp Glu Cys Val Phe Cys Gly Asp 
    1650                1655                1660                
Glu Cys Thr Gly Leu Leu Leu Gly Asp Leu Ala Arg Leu Glu Gln Met 
1665                1670                1675                1680
Val Met Ser Ile Asn Leu Thr Gly Pro Leu Pro Ala Pro Tyr Lys Met 
                1685                1690                1695    
Leu Tyr Gly Leu Glu Asn Met Thr Gln Glu Leu Lys His Leu Leu Ser 
            1700                1705                1710        
Pro Gln Arg Ala Pro Glu Arg Leu Ile Gln Leu Ala Glu Gly Asn Leu 
        1715                1720                1725            
Asn Thr Leu Val Thr Glu Met Asn Glu Leu Leu Thr Arg Ala Thr Lys 
    1730                1735                1740                
Val Thr Ala Asp Gly Glu Gln Thr Gly Gln Asp Ala Glu Arg Thr Asn 
1745                1750                1755                1760
Thr Arg Ala Lys Ser Leu Gly Glu Phe Ile Lys Glu Leu Ala Arg Asp 
                1765                1770                1775    
Ala Glu Ala Val Asn Glu Lys Ala Ile Lys Leu Asn Glu Thr Leu Gly 
            1780                1785                1790        
Thr Arg Asp Glu Ala Phe Glu Arg Asn Leu Glu Gly Leu Gln Lys Glu 
        1795                1800                1805            
Ile Asp Gln Met Ile Lys Glu Leu Arg Arg Lys Asn Leu Glu Thr Gln 
    1810                1815                1820                
Lys Glu Ile Ala Glu Asp Glu Leu Val Ala Ala Glu Ala Leu Leu Lys 
1825                1830                1835                1840
Lys Val Lys Lys Leu Phe Gly Glu Ser Arg Gly Glu Asn Glu Glu Met 
                1845                1850                1855    
Glu Lys Asp Leu Arg Glu Lys Leu Ala Asp Tyr Lys Asn Lys Val Asp 
            1860                1865                1870        
Asp Ala Trp Asp Leu Leu Arg Glu Ala Thr Asp Lys Ile Arg Glu Ala 
        1875                1880                1885            
Asn Arg Leu Phe Ala Val Asn Gln Lys Asn Met Thr Ala Leu Glu Lys 
    1890                1895                1900                
Lys Lys Glu Ala Val Glu Ser Gly Lys Arg Gln Ile Glu Asn Thr Leu 
1905                1910                1915                1920
Lys Glu Gly Asn Asp Ile Leu Asp Glu Ala Asn Arg Leu Ala Asp Glu 
                1925                1930                1935    
Ile Asn Ser Ile Ile Asp Tyr Val Glu Asp Ile Gln Thr Lys Leu Pro 
            1940                1945                1950        
Pro Met Ser Glu Glu Leu Asn Asp Lys Ile Asp Asp Leu Ser Gln Glu 
        1955                1960                1965            
Ile Lys Asp Arg Lys Leu Ala Glu Lys Val Ser Gln Ala Glu Ser His 
    1970                1975                1980                
Ala Ala Gln Leu Asn Asp Ser Ser Ala Val Leu Asp Gly Ile Leu Asp 
1985                1990                1995                2000
Glu Ala Lys Asn Ile Ser Phe Asn Ala Thr Ala Ala Phe Lys Ala Tyr 
                2005                2010                2015    
Ser Asn Ile Lys Asp Tyr Ile Asp Glu Ala Glu Lys Val Ala Lys Glu 
            2020                2025                2030        
Ala Lys Asp Leu Ala His Glu Ala Thr Lys Leu Ala Thr Gly Pro Arg 
        2035                2040                2045            
Gly Leu Leu Lys Glu Asp Ala Lys Gly Cys Leu Gln Lys Ser Phe Arg 
    2050                2055                2060                
Ile Leu Asn Glu Ala Lys Lys Leu Ala Asn Asp Val Lys Glu Asn Glu 
2065                2070                2075                2080
Asp His Leu Asn Gly Leu Lys Thr Arg Ile Glu Asn Ala Asp Ala Arg 
                2085                2090                2095    
Asn Gly Asp Leu Leu Arg Thr Leu Asn Asp Thr Leu Gly Lys Leu Ser 
            2100                2105                2110        
Ala Ile Pro Asn Asp Thr Ala Ala Lys Leu Gln Ala Val Lys Asp Lys 
        2115                2120                2125            
Ala Arg Gln Ala Asn Asp Thr Ala Lys Asp Val Leu Ala Gln Ile Thr 
    2130                2135                2140                
Glu Leu His Gln Asn Leu Asp Gly Leu Lys Lys Asn Tyr Asn Lys Leu 
2145                2150                2155                2160
Ala Asp Ser Val Ala Lys Thr Asn Ala Val Val Lys Asp Pro Ser Lys 
                2165                2170                2175    
Asn Lys Ile Ile Ala Asp Ala Asp Ala Thr Val Lys Asn Leu Glu Gln 
            2180                2185                2190        
Glu Ala Asp Arg Leu Ile Asp Lys Leu Lys Pro Ile Lys Glu Leu Glu 
        2195                2200                2205            
Asp Asn Leu Lys Lys Asn Ile Ser Glu Ile Lys Glu Leu Ile Asn Gln 
    2210                2215                2220                
Ala Arg Lys Gln Ala Asn Ser Ile Lys Val Ser Val Ser Ser Gly Gly 
2225                2230                2235                2240
Asp Cys Ile Arg Thr Tyr Lys Pro Glu Ile Lys Lys Gly Ser Tyr Asn 
                2245                2250                2255    
Asn Ile Val Val Asn Val Lys Thr Ala Val Ala Asp Asn Leu Leu Phe 
            2260                2265                2270        
Tyr Leu Gly Ser Ala Lys Phe Ile Asp Phe Leu Ala Ile Glu Met Arg 
        2275                2280                2285            
Lys Gly Lys Val Ser Phe Leu Trp Asp Val Gly Ser Gly Val Gly Arg 
    2290                2295                2300                
Val Glu Tyr Pro Asp Leu Thr Ile Asp Asp Ser Tyr Trp Tyr Arg Ile 
2305                2310                2315                2320
Val Ala Ser Arg Thr Gly Arg Asn Gly Thr Ile Ser Val Arg Ala Leu 
                2325                2330                2335    
Asp Gly Pro Lys Ala Ser Ile Val Pro Ser Thr His His Ser Thr Ser 
            2340                2345                2350        
Pro Pro Gly Tyr Thr Ile Leu Asp Val Asp Ala Asn Ala Met Leu Phe 
        2355                2360                2365            
Val Gly Gly Leu Thr Gly Lys Leu Lys Lys Ala Asp Ala Val Arg Val 
    2370                2375                2380                
Ile Thr Phe Thr Gly Cys Met Gly Glu Thr Tyr Phe Asp Asn Lys Pro 
2385                2390                2395                2400
Ile Gly Leu Trp Asn Phe Arg Glu Lys Glu Gly Asp Cys Lys Gly Cys 
                2405                2410                2415    
Thr Val Ser Pro Gln Val Glu Asp Ser Glu Gly Thr Ile Gln Phe Asp 
            2420                2425                2430        
Gly Glu Gly Tyr Ala Leu Val Ser Arg Pro Ile Arg Trp Tyr Pro Asn 
        2435                2440                2445            
Ile Ser Thr Val Met Phe Lys Phe Arg Thr Phe Ser Ser Ser Ala Leu 
    2450                2455                2460                
Leu Met Tyr Leu Ala Thr Arg Asp Leu Arg Asp Phe Met Ser Val Glu 
2465                2470                2475                2480
Leu Thr Asp Gly His Ile Lys Val Ser Tyr Asp Leu Gly Ser Gly Met 
                2485                2490                2495    
Ala Ser Val Val Ser Asn Gln Asn His Asn Asp Gly Lys Trp Lys Ser 
            2500                2505                2510        
Phe Thr Leu Ser Arg Ile Gln Lys Gln Ala Asn Ile Ser Ile Val Asp 
        2515                2520                2525            
Ile Asp Thr Asn Gln Glu Glu Asn Ile Ala Thr Ser Ser Ser Gly Asn 
    2530                2535                2540                
Asn Phe Gly Leu Asp Leu Lys Ala Asp Asp Lys Ile Tyr Phe Gly Gly 
2545                2550                2555                2560
Leu Pro Thr Leu Arg Asn Leu Arg Pro Glu Val Asn Leu Lys Lys Tyr 
                2565                2570                2575    
Ser Gly Cys Leu Lys Asp Ile Glu Ile Ser Arg Thr Pro Tyr Asn Ile 
            2580                2585                2590        
Leu Ser Ser Pro Asp Tyr Val Gly Val Thr Lys Gly Cys Ser Leu Glu 
        2595                2600                2605            
Asn Val Tyr Thr Val Ser Phe Pro Lys Pro Gly Phe Val Glu Leu Ser 
    2610                2615                2620                
Pro Val Pro Ile Asp Val Gly Thr Glu Ile Asn Leu Ser Phe Ser Thr 
2625                2630                2635                2640
Lys Asn Glu Ser Gly Ile Ile Leu Leu Gly Ser Gly Gly Thr Pro Ala 
                2645                2650                2655    
Pro Pro Arg Arg Lys Arg Arg Gln Thr Gly Gln Ala Tyr Tyr Ala Ile 
            2660                2665                2670        
Leu Leu Asn Arg Gly Arg Leu Glu Val His Leu Ser Thr Gly Ala Arg 
        2675                2680                2685            
Thr Met Arg Lys Ile Val Ile Arg Pro Glu Pro Asn Leu Phe His Asp 
    2690                2695                2700                
Gly Arg Glu His Ser Val His Val Glu Arg Thr Arg Gly Ile Phe Thr 
2705                2710                2715                2720
Val Gln Val Asp Glu Asn Arg Arg Tyr Met Gln Asn Leu Thr Val Glu 
                2725                2730                2735    
Gln Pro Ile Glu Val Lys Lys Leu Phe Val Gly Gly Ala Pro Pro Glu 
            2740                2745                2750        
Phe Gln Pro Ser Pro Leu Arg Asn Ile Pro Pro Phe Glu Gly Cys Ile 
        2755                2760                2765            
Trp Asn Leu Val Ile Asn Ser Val Pro Met Asp Phe Ala Arg Pro Val 
    2770                2775                2780                
Ser Phe Lys Asn Ala Asp Ile Gly Arg Cys Ala His Gln Lys Leu Arg 
2785                2790                2795                2800
Glu Asp Glu Asp Gly Ala Ala Pro Ala Glu Ile Val Ile Gln Pro Glu 
                2805                2810                2815    
Pro Val Pro Thr Pro Ala Phe Pro Thr Pro Thr Pro Val Leu Thr His 
            2820                2825                2830        
Gly Pro Cys Ala Ala Glu Ser Glu Pro Ala Leu Leu Ile Gly Ser Lys 
        2835                2840                2845            
Gln Phe Gly Leu Ser Arg Asn Ser His Ile Ala Ile Ala Phe Asp Asp 
    2850                2855                2860                
Thr Lys Val Lys Asn Arg Leu Thr Ile Glu Leu Glu Val Arg Thr Glu 
2865                2870                2875                2880
Ala Glu Ser Gly Leu Leu Phe Tyr Met Ala Arg Ile Asn His Ala Asp 
                2885                2890                2895    
Phe Ala Thr Val Gln Leu Arg Asn Gly Leu Pro Tyr Phe Ser Tyr Asp 
            2900                2905                2910        
Leu Gly Ser Gly Asp Thr His Thr Met Ile Pro Thr Lys Ile Asn Asp 
        2915                2920                2925            
Gly Gln Trp His Lys Ile Lys Ile Met Arg Ser Lys Gln Glu Gly Ile 
    2930                2935                2940                
Leu Tyr Val Asp Gly Ala Ser Asn Arg Thr Ile Ser Pro Lys Lys Ala 
2945                2950                2955                2960
Asp Ile Leu Asp Val Val Gly Met Leu Tyr Val Gly Gly Leu Pro Ile 
                2965                2970                2975    
Asn Tyr Thr Thr Arg Arg Ile Gly Pro Val Thr Tyr Ser Ile Asp Gly 
            2980                2985                2990        
Cys Val Arg Asn Leu His Met Ala Glu Ala Pro Ala Asp Leu Glu Gln 
        2995                3000                3005            
Pro Thr Ser Ser Phe His Val Gly Thr Cys Phe Ala Asn Ala Gln Arg 
    3010                3015                3020                
Gly Thr Tyr Phe Asp Gly Thr Gly Phe Ala Lys Ala Val Gly Gly Phe 
3025                3030                3035                3040
Lys Val Gly Leu Asp Leu Leu Val Glu Phe Glu Phe Arg Thr Thr Thr 
                3045                3050                3055    
Thr Thr Gly Val Leu Leu Gly Ile Ser Ser Gln Lys Met Asp Gly Met 
            3060                3065                3070        
Gly Ile Glu Met Ile Asp Glu Lys Leu Met Phe His Val Asp Asn Gly 
        3075                3080                3085            
Ala Gly Arg Phe Thr Ala Val Tyr Asp Ala Gly Val Pro Gly His Leu 
    3090                3095                3100                
Cys Asp Gly Gln Trp His Lys Val Thr Ala Asn Lys Ile Lys His Arg 
3105                3110                3115                3120
Ile Glu Leu Thr Val Asp Gly Asn Gln Val Glu Ala Gln Ser Pro Asn 
                3125                3130                3135    
Pro Ala Ser Thr Ser Ala Asp Thr Asn Asp Pro Val Phe Val Gly Gly 
            3140                3145                3150        
Phe Pro Asp Asp Leu Lys Gln Phe Gly Leu Thr Thr Ser Ile Pro Phe 
        3155                3160                3165            
Arg Gly Cys Ile Arg Ser Leu Lys Leu Thr Lys Gly Thr Gly Lys Pro 
    3170                3175                3180                
Leu Glu Val Asn Phe Ala Lys Ala Leu Glu Leu Arg Gly Val Gln Pro 
3185                3190                3195                3200
Val Ser Cys Pro Ala Asn 
                3205    

<210> 119
<211> 3212
<212> PRT
<213> Artificial Sequence


<220> 
<223> laminin subunit α2 isoform X4

<400> 119
Met Gln Trp Cys Phe Ser Val Leu Met Trp Arg Ser Gln Glu Thr Ser 
1               5                   10                  15      
Tyr Asn Ser Glu Lys His Leu Met Leu Ala Ala Val Ala Val Arg Leu 
            20                  25                  30          
Ala His Glu Leu Arg Ala Arg Gly Leu Phe Pro Ala Val Leu Asn Leu 
        35                  40                  45              
Ala Ser Asn Ala Leu Ile Thr Thr Asn Ala Thr Cys Gly Glu Lys Gly 
    50                  55                  60                  
Pro Glu Met Tyr Cys Lys Leu Val Glu His Val Pro Gly Gln Pro Val 
65                  70                  75                  80  
Arg Asn Pro Gln Cys Arg Ile Cys Asn Gln Asn Ser Ser Asn Pro Asn 
                85                  90                  95      
Gln Arg His Pro Ile Thr Asn Ala Ile Asp Gly Lys Asn Thr Trp Trp 
            100                 105                 110         
Gln Ser Pro Ser Ile Lys Asn Gly Ile Glu Tyr His Tyr Val Thr Ile 
        115                 120                 125             
Thr Leu Asp Leu Gln Gln Val Phe Gln Ile Ala Tyr Val Ile Val Lys 
    130                 135                 140                 
Ala Ala Asn Ser Pro Arg Pro Gly Asn Trp Ile Leu Glu Arg Ser Leu 
145                 150                 155                 160 
Asp Asp Val Glu Tyr Lys Pro Trp Gln Tyr His Ala Val Thr Asp Thr 
                165                 170                 175     
Glu Cys Leu Thr Leu Tyr Asn Ile Tyr Pro Arg Thr Gly Pro Pro Ser 
            180                 185                 190         
Tyr Ala Lys Asp Asp Glu Val Ile Cys Thr Ser Phe Tyr Ser Lys Ile 
        195                 200                 205             
His Pro Leu Glu Asn Gly Glu Ile His Ile Ser Leu Ile Asn Gly Arg 
    210                 215                 220                 
Pro Ser Ala Asp Asp Pro Ser Pro Glu Leu Leu Glu Phe Thr Ser Ala 
225                 230                 235                 240 
Arg Tyr Ile Arg Leu Arg Phe Gln Arg Ile Arg Thr Leu Asn Ala Asp 
                245                 250                 255     
Leu Met Met Phe Ala His Lys Asp Pro Arg Glu Ile Asp Pro Ile Val 
            260                 265                 270         
Thr Arg Arg Tyr Tyr Tyr Ser Val Lys Asp Ile Ser Val Gly Gly Met 
        275                 280                 285             
Cys Ile Cys Tyr Gly His Ala Arg Ala Cys Pro Leu Asp Pro Ala Thr 
    290                 295                 300                 
Asn Lys Ser Arg Cys Glu Cys Glu His Asn Thr Cys Gly Asp Ser Cys 
305                 310                 315                 320 
Asp Gln Cys Cys Pro Gly Phe His Gln Lys Pro Trp Arg Ala Gly Thr 
                325                 330                 335     
Phe Leu Thr Lys Thr Glu Cys Glu Ala Cys Asn Cys His Gly Lys Ala 
            340                 345                 350         
Glu Glu Cys Tyr Tyr Asp Glu Asn Val Ala Arg Arg Asn Leu Ser Leu 
        355                 360                 365             
Asn Ile Arg Gly Lys Tyr Ile Gly Gly Gly Val Cys Ile Asn Cys Thr 
    370                 375                 380                 
Gln Asn Thr Ala Gly Ile Asn Cys Glu Thr Cys Thr Asp Gly Phe Phe 
385                 390                 395                 400 
Arg Pro Lys Gly Val Ser Pro Asn Tyr Pro Arg Pro Cys Gln Pro Cys 
                405                 410                 415     
His Cys Asp Pro Ile Gly Ser Leu Asn Glu Val Cys Val Lys Asp Glu 
            420                 425                 430         
Lys His Ala Arg Arg Gly Leu Ala Pro Gly Ser Cys His Cys Lys Thr 
        435                 440                 445             
Gly Phe Gly Gly Val Ser Cys Asp Arg Cys Ala Arg Gly Tyr Thr Gly 
    450                 455                 460                 
Tyr Pro Asp Cys Lys Ala Cys Asn Cys Ser Gly Leu Gly Ser Lys Asn 
465                 470                 475                 480 
Glu Asp Pro Cys Phe Gly Pro Cys Ile Cys Lys Glu Asn Val Glu Gly 
                485                 490                 495     
Gly Asp Cys Ser Arg Cys Lys Ser Gly Phe Phe Asn Leu Gln Glu Asp 
            500                 505                 510         
Asn Trp Lys Gly Cys Asp Glu Cys Phe Cys Ser Gly Val Ser Asn Arg 
        515                 520                 525             
Cys Gln Ser Ser Tyr Trp Thr Tyr Gly Lys Ile Gln Asp Met Ser Gly 
    530                 535                 540                 
Trp Tyr Leu Thr Asp Leu Pro Gly Arg Ile Arg Val Ala Pro Gln Gln 
545                 550                 555                 560 
Asp Asp Leu Asp Ser Pro Gln Gln Ile Ser Ile Ser Asn Ala Glu Ala 
                565                 570                 575     
Arg Gln Ala Leu Pro His Ser Tyr Tyr Trp Ser Ala Pro Ala Pro Tyr 
            580                 585                 590         
Leu Gly Asn Lys Leu Pro Ala Val Gly Gly Gln Leu Thr Phe Thr Ile 
        595                 600                 605             
Ser Tyr Asp Leu Glu Glu Glu Glu Glu Asp Thr Glu Arg Val Leu Gln 
    610                 615                 620                 
Leu Met Ile Ile Leu Glu Gly Asn Asp Leu Ser Ile Ser Thr Ala Gln 
625                 630                 635                 640 
Asp Glu Val Tyr Leu His Pro Ser Glu Glu His Thr Asn Val Leu Leu 
                645                 650                 655     
Leu Lys Glu Glu Ser Phe Thr Ile His Gly Thr His Phe Pro Val Arg 
            660                 665                 670         
Arg Lys Glu Phe Met Thr Val Leu Ala Asn Leu Lys Arg Val Leu Leu 
        675                 680                 685             
Gln Ile Thr Tyr Ser Phe Gly Met Asp Ala Ile Phe Arg Leu Ser Ser 
    690                 695                 700                 
Val Asn Leu Glu Ser Ala Val Ser Tyr Pro Thr Asp Gly Ser Ile Ala 
705                 710                 715                 720 
Ala Ala Val Glu Val Cys Gln Cys Pro Pro Gly Tyr Thr Gly Ser Ser 
                725                 730                 735     
Cys Glu Ser Cys Trp Pro Arg His Arg Arg Val Asn Gly Thr Ile Phe 
            740                 745                 750         
Gly Gly Ile Cys Glu Pro Cys Gln Cys Phe Gly His Ala Glu Ser Cys 
        755                 760                 765             
Asp Asp Val Thr Gly Glu Cys Leu Asn Cys Lys Asp His Thr Gly Gly 
    770                 775                 780                 
Pro Tyr Cys Asp Lys Cys Leu Pro Gly Phe Tyr Gly Glu Pro Thr Lys 
785                 790                 795                 800 
Gly Thr Ser Glu Asp Cys Gln Pro Cys Ala Cys Pro Leu Asn Ile Pro 
                805                 810                 815     
Ser Asn Asn Phe Ser Pro Thr Cys His Leu Asp Arg Ser Leu Gly Leu 
            820                 825                 830         
Ile Cys Asp Gly Cys Pro Val Gly Tyr Thr Gly Pro Arg Cys Glu Arg 
        835                 840                 845             
Cys Ala Glu Gly Tyr Phe Gly Gln Pro Ser Val Pro Gly Gly Ser Cys 
    850                 855                 860                 
Gln Pro Cys Gln Cys Asn Asp Asn Leu Asp Phe Ser Ile Pro Gly Ser 
865                 870                 875                 880 
Cys Asp Ser Leu Ser Gly Ser Cys Leu Ile Cys Lys Pro Gly Thr Thr 
                885                 890                 895     
Gly Arg Tyr Cys Glu Leu Cys Ala Asp Gly Tyr Phe Gly Asp Ala Val 
            900                 905                 910         
Asp Ala Lys Asn Cys Gln Pro Cys Arg Cys Asn Ala Gly Gly Ser Phe 
        915                 920                 925             
Ser Glu Val Cys His Ser Gln Thr Gly Gln Cys Glu Cys Arg Ala Asn 
    930                 935                 940                 
Val Gln Gly Gln Arg Cys Asp Lys Cys Lys Pro Asn Met Trp Arg Asp 
945                 950                 955                 960 
Pro Glu Lys Arg Phe Cys Val Leu Cys Asp Cys Asp Pro Val Gly Ser 
                965                 970                 975     
Val Ser Pro Gln Cys Asp Ile Thr Gly Arg Cys Val Cys Lys Ser Gly 
            980                 985                 990         
Phe Val Gly Lys Gln Cys Asn Leu Gly Arg Gln Val His Gln Gln Glu 
        995                 1000                1005            
Glu Gln Pro Arg Arg Ala Gln Arg Val Leu Gly Ser Pro Gln Arg Trp 
    1010                1015                1020                
Ala Ile Gly Ser Ser Ser Gly Cys Pro Arg Gly Ala Tyr Arg Ala Pro 
1025                1030                1035                1040
Ala Pro Ala Gly Thr Phe Gly Leu Gln Ser Ala Arg Gly Cys Val Pro 
                1045                1050                1055    
Cys Asn Cys Asn Ser Phe Gly Ser Lys Ser Phe Asp Cys Glu Glu Ser 
            1060                1065                1070        
Gly Gln Cys Trp Cys Gln Pro Gly Val Thr Gly Lys Lys Cys Asp Arg 
        1075                1080                1085            
Cys Ala His Gly Tyr Phe Asn Phe Gln Glu Gly Gly Cys Thr Ala Cys 
    1090                1095                1100                
Glu Cys Ser His Leu Gly Asn Asn Cys Asp Pro Lys Thr Gly Arg Cys 
1105                1110                1115                1120
Ile Cys Pro Pro Asn Thr Ile Gly Glu Lys Cys Ser Lys Cys Ala Pro 
                1125                1130                1135    
Asn Thr Trp Gly His Ser Ile Thr Thr Gly Cys Lys Ala Cys Asn Cys 
            1140                1145                1150        
Ser Thr Val Gly Ser Leu Asp Phe Gln Cys Asn Val Asn Thr Gly Gln 
        1155                1160                1165            
Cys Asn Cys His Pro Lys Phe Ser Gly Ala Lys Cys Thr Glu Cys Ser 
    1170                1175                1180                
Arg Gly His Trp Asn Tyr Pro Arg Cys Asn Leu Cys Asp Cys Phe Leu 
1185                1190                1195                1200
Pro Gly Thr Asp Ala Thr Thr Cys Asp Ser Glu Thr Lys Lys Cys Ser 
                1205                1210                1215    
Cys Ser Asp Gln Thr Gly Gln Cys Thr Cys Lys Val Asn Val Glu Gly 
            1220                1225                1230        
Ile His Cys Asp Arg Cys Arg Pro Gly Lys Phe Gly Leu Asp Ala Lys 
        1235                1240                1245            
Asn Pro Leu Gly Cys Ser Ser Cys Tyr Cys Phe Gly Thr Thr Thr Gln 
    1250                1255                1260                
Cys Ser Glu Ala Lys Gly Leu Ile Arg Thr Trp Val Thr Leu Lys Ala 
1265                1270                1275                1280
Glu Gln Thr Ile Leu Pro Leu Val Asp Glu Ala Leu Gln His Thr Thr 
                1285                1290                1295    
Thr Lys Gly Ile Val Phe Gln His Pro Glu Ile Val Ala His Met Asp 
            1300                1305                1310        
Leu Met Arg Glu Asp Leu His Leu Glu Pro Phe Tyr Trp Lys Leu Pro 
        1315                1320                1325            
Glu Gln Phe Glu Gly Lys Lys Leu Met Ala Tyr Gly Gly Lys Leu Lys 
    1330                1335                1340                
Tyr Ala Ile Tyr Phe Glu Ala Arg Glu Glu Thr Gly Phe Ser Thr Tyr 
1345                1350                1355                1360
Asn Pro Gln Val Ile Ile Arg Gly Gly Thr Pro Thr His Ala Arg Ile 
                1365                1370                1375    
Ile Val Arg His Met Ala Ala Pro Leu Ile Gly Gln Leu Thr Arg His 
            1380                1385                1390        
Glu Ile Glu Met Thr Glu Lys Glu Trp Lys Tyr Tyr Gly Asp Asp Pro 
        1395                1400                1405            
Arg Val His Arg Thr Val Thr Arg Glu Asp Phe Leu Asp Ile Leu Tyr 
    1410                1415                1420                
Asp Ile His Tyr Ile Leu Ile Lys Ala Thr Tyr Gly Asn Phe Met Arg 
1425                1430                1435                1440
Gln Ser Arg Ile Ser Glu Ile Ser Met Glu Val Ala Glu Gln Gly Arg 
                1445                1450                1455    
Gly Thr Thr Met Thr Pro Pro Ala Asp Leu Ile Glu Lys Cys Asp Cys 
            1460                1465                1470        
Pro Leu Gly Tyr Ser Gly Leu Ser Cys Glu Ala Cys Leu Pro Gly Phe 
        1475                1480                1485            
Tyr Arg Leu Arg Ser Gln Pro Gly Gly Arg Thr Pro Gly Pro Thr Leu 
    1490                1495                1500                
Gly Thr Cys Val Pro Cys Gln Cys Asn Gly His Ser Ser Leu Cys Asp 
1505                1510                1515                1520
Pro Glu Thr Ser Ile Cys Gln Asn Cys Gln His His Thr Ala Gly Asp 
                1525                1530                1535    
Phe Cys Glu Arg Cys Ala Leu Gly Tyr Tyr Gly Ile Val Lys Gly Leu 
            1540                1545                1550        
Pro Asn Asp Cys Gln Gln Cys Ala Cys Pro Leu Ile Ser Ser Ser Asn 
        1555                1560                1565            
Asn Phe Ser Pro Ser Cys Val Ala Glu Gly Leu Asp Asp Tyr Arg Cys 
    1570                1575                1580                
Thr Ala Cys Pro Arg Gly Tyr Glu Gly Gln Tyr Cys Glu Arg Cys Ala 
1585                1590                1595                1600
Pro Gly Tyr Thr Gly Ser Pro Gly Asn Pro Gly Gly Ser Cys Gln Glu 
                1605                1610                1615    
Cys Glu Cys Asp Pro Tyr Gly Ser Leu Pro Val Pro Cys Asp Pro Val 
            1620                1625                1630        
Thr Gly Phe Cys Thr Cys Arg Pro Gly Ala Thr Gly Arg Lys Cys Asp 
        1635                1640                1645            
Gly Cys Lys His Trp His Ala Arg Glu Gly Trp Glu Cys Val Phe Cys 
    1650                1655                1660                
Gly Asp Glu Cys Thr Gly Leu Leu Leu Gly Asp Leu Ala Arg Leu Glu 
1665                1670                1675                1680
Gln Met Val Met Ser Ile Asn Leu Thr Gly Pro Leu Pro Ala Pro Tyr 
                1685                1690                1695    
Lys Met Leu Tyr Gly Leu Glu Asn Met Thr Gln Glu Leu Lys His Leu 
            1700                1705                1710        
Leu Ser Pro Gln Arg Ala Pro Glu Arg Leu Ile Gln Leu Ala Glu Gly 
        1715                1720                1725            
Asn Leu Asn Thr Leu Val Thr Glu Met Asn Glu Leu Leu Thr Arg Ala 
    1730                1735                1740                
Thr Lys Val Thr Ala Asp Gly Glu Gln Thr Gly Gln Asp Ala Glu Arg 
1745                1750                1755                1760
Thr Asn Thr Arg Ala Lys Ser Leu Gly Glu Phe Ile Lys Glu Leu Ala 
                1765                1770                1775    
Arg Asp Ala Glu Ala Val Asn Glu Lys Ala Ile Lys Leu Asn Glu Thr 
            1780                1785                1790        
Leu Gly Thr Arg Asp Glu Ala Phe Glu Arg Asn Leu Glu Gly Leu Gln 
        1795                1800                1805            
Lys Glu Ile Asp Gln Met Ile Lys Glu Leu Arg Arg Lys Asn Leu Glu 
    1810                1815                1820                
Thr Gln Lys Glu Ile Ala Glu Asp Glu Leu Val Ala Ala Glu Ala Leu 
1825                1830                1835                1840
Leu Lys Lys Val Lys Lys Leu Phe Gly Glu Ser Arg Gly Glu Asn Glu 
                1845                1850                1855    
Glu Met Glu Lys Asp Leu Arg Glu Lys Leu Ala Asp Tyr Lys Asn Lys 
            1860                1865                1870        
Val Asp Asp Ala Trp Asp Leu Leu Arg Glu Ala Thr Asp Lys Ile Arg 
        1875                1880                1885            
Glu Ala Asn Arg Leu Phe Ala Val Asn Gln Lys Asn Met Thr Ala Leu 
    1890                1895                1900                
Glu Lys Lys Lys Glu Ala Val Glu Ser Gly Lys Arg Gln Ile Glu Asn 
1905                1910                1915                1920
Thr Leu Lys Glu Gly Asn Asp Ile Leu Asp Glu Ala Asn Arg Leu Ala 
                1925                1930                1935    
Asp Glu Ile Asn Ser Ile Ile Asp Tyr Val Glu Asp Ile Gln Thr Lys 
            1940                1945                1950        
Leu Pro Pro Met Ser Glu Glu Leu Asn Asp Lys Ile Asp Asp Leu Ser 
        1955                1960                1965            
Gln Glu Ile Lys Asp Arg Lys Leu Ala Glu Lys Val Ser Gln Ala Glu 
    1970                1975                1980                
Ser His Ala Ala Gln Leu Asn Asp Ser Ser Ala Val Leu Asp Gly Ile 
1985                1990                1995                2000
Leu Asp Glu Ala Lys Asn Ile Ser Phe Asn Ala Thr Ala Ala Phe Lys 
                2005                2010                2015    
Ala Tyr Ser Asn Ile Lys Asp Tyr Ile Asp Glu Ala Glu Lys Val Ala 
            2020                2025                2030        
Lys Glu Ala Lys Asp Leu Ala His Glu Ala Thr Lys Leu Ala Thr Gly 
        2035                2040                2045            
Pro Arg Gly Leu Leu Lys Glu Asp Ala Lys Gly Cys Leu Gln Lys Ser 
    2050                2055                2060                
Phe Arg Ile Leu Asn Glu Ala Lys Lys Leu Ala Asn Asp Val Lys Glu 
2065                2070                2075                2080
Asn Glu Asp His Leu Asn Gly Leu Lys Thr Arg Ile Glu Asn Ala Asp 
                2085                2090                2095    
Ala Arg Asn Gly Asp Leu Leu Arg Thr Leu Asn Asp Thr Leu Gly Lys 
            2100                2105                2110        
Leu Ser Ala Ile Pro Asn Asp Thr Ala Ala Lys Leu Gln Ala Val Lys 
        2115                2120                2125            
Asp Lys Ala Arg Gln Ala Asn Asp Thr Ala Lys Asp Val Leu Ala Gln 
    2130                2135                2140                
Ile Thr Glu Leu His Gln Asn Leu Asp Gly Leu Lys Lys Asn Tyr Asn 
2145                2150                2155                2160
Lys Leu Ala Asp Ser Val Ala Lys Thr Asn Ala Val Val Lys Asp Pro 
                2165                2170                2175    
Ser Lys Asn Lys Ile Ile Ala Asp Ala Asp Ala Thr Val Lys Asn Leu 
            2180                2185                2190        
Glu Gln Glu Ala Asp Arg Leu Ile Asp Lys Leu Lys Pro Ile Lys Glu 
        2195                2200                2205            
Leu Glu Asp Asn Leu Lys Lys Asn Ile Ser Glu Ile Lys Glu Leu Ile 
    2210                2215                2220                
Asn Gln Ala Arg Lys Gln Ala Asn Ser Ile Lys Val Ser Val Ser Ser 
2225                2230                2235                2240
Gly Gly Asp Cys Ile Arg Thr Tyr Lys Pro Glu Ile Lys Lys Gly Ser 
                2245                2250                2255    
Tyr Asn Asn Ile Val Val Asn Val Lys Thr Ala Val Ala Asp Asn Leu 
            2260                2265                2270        
Leu Phe Tyr Leu Gly Ser Ala Lys Phe Ile Asp Phe Leu Ala Ile Glu 
        2275                2280                2285            
Met Arg Lys Gly Lys Val Ser Phe Leu Trp Asp Val Gly Ser Gly Val 
    2290                2295                2300                
Gly Arg Val Glu Tyr Pro Asp Leu Thr Ile Asp Asp Ser Tyr Trp Tyr 
2305                2310                2315                2320
Arg Ile Val Ala Ser Arg Thr Gly Arg Asn Gly Thr Ile Ser Val Arg 
                2325                2330                2335    
Ala Leu Asp Gly Pro Lys Ala Ser Ile Val Pro Ser Thr His His Ser 
            2340                2345                2350        
Thr Ser Pro Pro Gly Tyr Thr Ile Leu Asp Val Asp Ala Asn Ala Met 
        2355                2360                2365            
Leu Phe Val Gly Gly Leu Thr Gly Lys Leu Lys Lys Ala Asp Ala Val 
    2370                2375                2380                
Arg Val Ile Thr Phe Thr Gly Cys Met Gly Glu Thr Tyr Phe Asp Asn 
2385                2390                2395                2400
Lys Pro Ile Gly Leu Trp Asn Phe Arg Glu Lys Glu Gly Asp Cys Lys 
                2405                2410                2415    
Gly Cys Thr Val Ser Pro Gln Val Glu Asp Ser Glu Gly Thr Ile Gln 
            2420                2425                2430        
Phe Asp Gly Glu Gly Tyr Ala Leu Val Ser Arg Pro Ile Arg Trp Tyr 
        2435                2440                2445            
Pro Asn Ile Ser Thr Val Met Phe Lys Phe Arg Thr Phe Ser Ser Ser 
    2450                2455                2460                
Ala Leu Leu Met Tyr Leu Ala Thr Arg Asp Leu Arg Asp Phe Met Ser 
2465                2470                2475                2480
Val Glu Leu Thr Asp Gly His Ile Lys Val Ser Tyr Asp Leu Gly Ser 
                2485                2490                2495    
Gly Met Ala Ser Val Val Ser Asn Gln Asn His Asn Asp Gly Lys Trp 
            2500                2505                2510        
Lys Ser Phe Thr Leu Ser Arg Ile Gln Lys Gln Ala Asn Ile Ser Ile 
        2515                2520                2525            
Val Asp Ile Asp Thr Asn Gln Glu Glu Asn Ile Ala Thr Ser Ser Ser 
    2530                2535                2540                
Gly Asn Asn Phe Gly Leu Asp Leu Lys Ala Asp Asp Lys Ile Tyr Phe 
2545                2550                2555                2560
Gly Gly Leu Pro Thr Leu Arg Asn Leu Ser Met Lys Ala Arg Pro Glu 
                2565                2570                2575    
Val Asn Leu Lys Lys Tyr Ser Gly Cys Leu Lys Asp Ile Glu Ile Ser 
            2580                2585                2590        
Arg Thr Pro Tyr Asn Ile Leu Ser Ser Pro Asp Tyr Val Gly Val Thr 
        2595                2600                2605            
Lys Gly Cys Ser Leu Glu Asn Val Tyr Thr Val Ser Phe Pro Lys Pro 
    2610                2615                2620                
Gly Phe Val Glu Leu Ser Pro Val Pro Ile Asp Val Gly Thr Glu Ile 
2625                2630                2635                2640
Asn Leu Ser Phe Ser Thr Lys Asn Glu Ser Gly Ile Ile Leu Leu Gly 
                2645                2650                2655    
Ser Gly Gly Thr Pro Ala Pro Pro Arg Arg Lys Arg Arg Gln Thr Gly 
            2660                2665                2670        
Gln Ala Tyr Tyr Ala Ile Leu Leu Asn Arg Gly Arg Leu Glu Val His 
        2675                2680                2685            
Leu Ser Thr Gly Ala Arg Thr Met Arg Lys Ile Val Ile Arg Pro Glu 
    2690                2695                2700                
Pro Asn Leu Phe His Asp Gly Arg Glu His Ser Val His Val Glu Arg 
2705                2710                2715                2720
Thr Arg Gly Ile Phe Thr Val Gln Val Asp Glu Asn Arg Arg Tyr Met 
                2725                2730                2735    
Gln Asn Leu Thr Val Glu Gln Pro Ile Glu Val Lys Lys Leu Phe Val 
            2740                2745                2750        
Gly Gly Ala Pro Pro Glu Phe Gln Pro Ser Pro Leu Arg Asn Ile Pro 
        2755                2760                2765            
Pro Phe Glu Gly Cys Ile Trp Asn Leu Val Ile Asn Ser Val Pro Met 
    2770                2775                2780                
Asp Phe Ala Arg Pro Val Ser Phe Lys Asn Ala Asp Ile Gly Arg Cys 
2785                2790                2795                2800
Ala His Gln Lys Leu Arg Glu Asp Glu Asp Gly Ala Ala Pro Ala Glu 
                2805                2810                2815    
Ile Val Ile Gln Pro Glu Pro Val Pro Thr Pro Ala Phe Pro Thr Pro 
            2820                2825                2830        
Thr Pro Val Leu Thr His Gly Pro Cys Ala Ala Glu Ser Glu Pro Ala 
        2835                2840                2845            
Leu Leu Ile Gly Ser Lys Gln Phe Gly Leu Ser Arg Asn Ser His Ile 
    2850                2855                2860                
Ala Ile Ala Phe Asp Asp Thr Lys Val Lys Asn Arg Leu Thr Ile Glu 
2865                2870                2875                2880
Leu Glu Val Arg Thr Glu Ala Glu Ser Gly Leu Leu Phe Tyr Met Ala 
                2885                2890                2895    
Arg Ile Asn His Ala Asp Phe Ala Thr Val Gln Leu Arg Asn Gly Leu 
            2900                2905                2910        
Pro Tyr Phe Ser Tyr Asp Leu Gly Ser Gly Asp Thr His Thr Met Ile 
        2915                2920                2925            
Pro Thr Lys Ile Asn Asp Gly Gln Trp His Lys Ile Lys Ile Met Arg 
    2930                2935                2940                
Ser Lys Gln Glu Gly Ile Leu Tyr Val Asp Gly Ala Ser Asn Arg Thr 
2945                2950                2955                2960
Ile Ser Pro Lys Lys Ala Asp Ile Leu Asp Val Val Gly Met Leu Tyr 
                2965                2970                2975    
Val Gly Gly Leu Pro Ile Asn Tyr Thr Thr Arg Arg Ile Gly Pro Val 
            2980                2985                2990        
Thr Tyr Ser Ile Asp Gly Cys Val Arg Asn Leu His Met Ala Glu Ala 
        2995                3000                3005            
Pro Ala Asp Leu Glu Gln Pro Thr Ser Ser Phe His Val Gly Thr Cys 
    3010                3015                3020                
Phe Ala Asn Ala Gln Arg Gly Thr Tyr Phe Asp Gly Thr Gly Phe Ala 
3025                3030                3035                3040
Lys Ala Val Gly Gly Phe Lys Val Gly Leu Asp Leu Leu Val Glu Phe 
                3045                3050                3055    
Glu Phe Arg Thr Thr Thr Thr Thr Gly Val Leu Leu Gly Ile Ser Ser 
            3060                3065                3070        
Gln Lys Met Asp Gly Met Gly Ile Glu Met Ile Asp Glu Lys Leu Met 
        3075                3080                3085            
Phe His Val Asp Asn Gly Ala Gly Arg Phe Thr Ala Val Tyr Asp Ala 
    3090                3095                3100                
Gly Val Pro Gly His Leu Cys Asp Gly Gln Trp His Lys Val Thr Ala 
3105                3110                3115                3120
Asn Lys Ile Lys His Arg Ile Glu Leu Thr Val Asp Gly Asn Gln Val 
                3125                3130                3135    
Glu Ala Gln Ser Pro Asn Pro Ala Ser Thr Ser Ala Asp Thr Asn Asp 
            3140                3145                3150        
Pro Val Phe Val Gly Gly Phe Pro Asp Asp Leu Lys Gln Phe Gly Leu 
        3155                3160                3165            
Thr Thr Ser Ile Pro Phe Arg Gly Cys Ile Arg Ser Leu Lys Leu Thr 
    3170                3175                3180                
Lys Gly Thr Gly Lys Pro Leu Glu Val Asn Phe Ala Lys Ala Leu Glu 
3185                3190                3195                3200
Leu Arg Gly Val Gln Pro Val Ser Cys Pro Ala Asn 
                3205                3210        

<210> 120
<211> 2587
<212> PRT
<213> Artificial Sequence


<220> 
<223> laminin subunit α2 isoform X5

<400> 120
Met Ile Ile Leu Glu Gly Asn Asp Leu Ser Ile Ser Thr Ala Gln Asp 
1               5                   10                  15      
Glu Val Tyr Leu His Pro Ser Glu Glu His Thr Asn Val Leu Leu Leu 
            20                  25                  30          
Lys Glu Glu Ser Phe Thr Ile His Gly Thr His Phe Pro Val Arg Arg 
        35                  40                  45              
Lys Glu Phe Met Thr Val Leu Ala Asn Leu Lys Arg Val Leu Leu Gln 
    50                  55                  60                  
Ile Thr Tyr Ser Phe Gly Met Asp Ala Ile Phe Arg Leu Ser Ser Val 
65                  70                  75                  80  
Asn Leu Glu Ser Ala Val Ser Tyr Pro Thr Asp Gly Ser Ile Ala Ala 
                85                  90                  95      
Ala Val Glu Val Cys Gln Cys Pro Pro Gly Tyr Thr Gly Ser Ser Cys 
            100                 105                 110         
Glu Ser Cys Trp Pro Arg His Arg Arg Val Asn Gly Thr Ile Phe Gly 
        115                 120                 125             
Gly Ile Cys Glu Pro Cys Gln Cys Phe Gly His Ala Glu Ser Cys Asp 
    130                 135                 140                 
Asp Val Thr Gly Glu Cys Leu Asn Cys Lys Asp His Thr Gly Gly Pro 
145                 150                 155                 160 
Tyr Cys Asp Lys Cys Leu Pro Gly Phe Tyr Gly Glu Pro Thr Lys Gly 
                165                 170                 175     
Thr Ser Glu Asp Cys Gln Pro Cys Ala Cys Pro Leu Asn Ile Pro Ser 
            180                 185                 190         
Asn Asn Phe Ser Pro Thr Cys His Leu Asp Arg Ser Leu Gly Leu Ile 
        195                 200                 205             
Cys Asp Gly Cys Pro Val Gly Tyr Thr Gly Pro Arg Cys Glu Arg Cys 
    210                 215                 220                 
Ala Glu Gly Tyr Phe Gly Gln Pro Ser Val Pro Gly Gly Ser Cys Gln 
225                 230                 235                 240 
Pro Cys Gln Cys Asn Asp Asn Leu Asp Phe Ser Ile Pro Gly Ser Cys 
                245                 250                 255     
Asp Ser Leu Ser Gly Ser Cys Leu Ile Cys Lys Pro Gly Thr Thr Gly 
            260                 265                 270         
Arg Tyr Cys Glu Leu Cys Ala Asp Gly Tyr Phe Gly Asp Ala Val Asp 
        275                 280                 285             
Ala Lys Asn Cys Gln Pro Cys Arg Cys Asn Ala Gly Gly Ser Phe Ser 
    290                 295                 300                 
Glu Val Cys His Ser Gln Thr Gly Gln Cys Glu Cys Arg Ala Asn Val 
305                 310                 315                 320 
Gln Gly Gln Arg Cys Asp Lys Cys Lys Pro Asn Met Trp Arg Asp Pro 
                325                 330                 335     
Glu Lys Arg Phe Cys Val Leu Cys Asp Cys Asp Pro Val Gly Ser Val 
            340                 345                 350         
Ser Pro Gln Cys Asp Ile Thr Gly Arg Cys Val Cys Lys Ser Gly Phe 
        355                 360                 365             
Val Gly Lys Gln Cys Asn Leu Gly Arg Gln Val His Gln Gln Glu Glu 
    370                 375                 380                 
Gln Pro Arg Arg Ala Gln Arg Val Leu Gly Ser Pro Gln Arg Trp Ala 
385                 390                 395                 400 
Ile Gly Ser Ser Ser Gly Cys Pro Arg Gly Ala Tyr Arg Ala Pro Ala 
                405                 410                 415     
Pro Ala Gly Thr Phe Gly Leu Gln Ser Ala Arg Gly Cys Val Pro Cys 
            420                 425                 430         
Asn Cys Asn Ser Phe Gly Ser Lys Ser Phe Asp Cys Glu Glu Ser Gly 
        435                 440                 445             
Gln Cys Trp Cys Gln Pro Gly Val Thr Gly Lys Lys Cys Asp Arg Cys 
    450                 455                 460                 
Ala His Gly Tyr Phe Asn Phe Gln Glu Gly Gly Cys Thr Ala Cys Glu 
465                 470                 475                 480 
Cys Ser His Leu Gly Asn Asn Cys Asp Pro Lys Thr Gly Arg Cys Ile 
                485                 490                 495     
Cys Pro Pro Asn Thr Ile Gly Glu Lys Cys Ser Lys Cys Ala Pro Asn 
            500                 505                 510         
Thr Trp Gly His Ser Ile Thr Thr Gly Cys Lys Ala Cys Asn Cys Ser 
        515                 520                 525             
Thr Val Gly Ser Leu Asp Phe Gln Cys Asn Val Asn Thr Gly Gln Cys 
    530                 535                 540                 
Asn Cys His Pro Lys Phe Ser Gly Ala Lys Cys Thr Glu Cys Ser Arg 
545                 550                 555                 560 
Gly His Trp Asn Tyr Pro Arg Cys Asn Leu Cys Asp Cys Phe Leu Pro 
                565                 570                 575     
Gly Thr Asp Ala Thr Thr Cys Asp Ser Glu Thr Lys Lys Cys Ser Cys 
            580                 585                 590         
Ser Asp Gln Thr Gly Gln Cys Thr Cys Lys Val Asn Val Glu Gly Ile 
        595                 600                 605             
His Cys Asp Arg Cys Arg Pro Gly Lys Phe Gly Leu Asp Ala Lys Asn 
    610                 615                 620                 
Pro Leu Gly Cys Ser Ser Cys Tyr Cys Phe Gly Thr Thr Thr Gln Cys 
625                 630                 635                 640 
Ser Glu Ala Lys Gly Leu Ile Arg Thr Trp Val Thr Leu Lys Ala Glu 
                645                 650                 655     
Gln Thr Ile Leu Pro Leu Val Asp Glu Ala Leu Gln His Thr Thr Thr 
            660                 665                 670         
Lys Gly Ile Val Phe Gln His Pro Glu Ile Val Ala His Met Asp Leu 
        675                 680                 685             
Met Arg Glu Asp Leu His Leu Glu Pro Phe Tyr Trp Lys Leu Pro Glu 
    690                 695                 700                 
Gln Phe Glu Gly Lys Lys Leu Met Ala Tyr Gly Gly Lys Leu Lys Tyr 
705                 710                 715                 720 
Ala Ile Tyr Phe Glu Ala Arg Glu Glu Thr Gly Phe Ser Thr Tyr Asn 
                725                 730                 735     
Pro Gln Val Ile Ile Arg Gly Gly Thr Pro Thr His Ala Arg Ile Ile 
            740                 745                 750         
Val Arg His Met Ala Ala Pro Leu Ile Gly Gln Leu Thr Arg His Glu 
        755                 760                 765             
Ile Glu Met Thr Glu Lys Glu Trp Lys Tyr Tyr Gly Asp Asp Pro Arg 
    770                 775                 780                 
Val His Arg Thr Val Thr Arg Glu Asp Phe Leu Asp Ile Leu Tyr Asp 
785                 790                 795                 800 
Ile His Tyr Ile Leu Ile Lys Ala Thr Tyr Gly Asn Phe Met Arg Gln 
                805                 810                 815     
Ser Arg Ile Ser Glu Ile Ser Met Glu Val Ala Glu Gln Gly Arg Gly 
            820                 825                 830         
Thr Thr Met Thr Pro Pro Ala Asp Leu Ile Glu Lys Cys Asp Cys Pro 
        835                 840                 845             
Leu Gly Tyr Ser Gly Leu Ser Cys Glu Ala Cys Leu Pro Gly Phe Tyr 
    850                 855                 860                 
Arg Leu Arg Ser Gln Pro Gly Gly Arg Thr Pro Gly Pro Thr Leu Gly 
865                 870                 875                 880 
Thr Cys Val Pro Cys Gln Cys Asn Gly His Ser Ser Leu Cys Asp Pro 
                885                 890                 895     
Glu Thr Ser Ile Cys Gln Asn Cys Gln His His Thr Ala Gly Asp Phe 
            900                 905                 910         
Cys Glu Arg Cys Ala Leu Gly Tyr Tyr Gly Ile Val Lys Gly Leu Pro 
        915                 920                 925             
Asn Asp Cys Gln Gln Cys Ala Cys Pro Leu Ile Ser Ser Ser Asn Asn 
    930                 935                 940                 
Phe Ser Pro Ser Cys Val Ala Glu Gly Leu Asp Asp Tyr Arg Cys Thr 
945                 950                 955                 960 
Ala Cys Pro Arg Gly Tyr Glu Gly Gln Tyr Cys Glu Arg Cys Ala Pro 
                965                 970                 975     
Gly Tyr Thr Gly Ser Pro Gly Asn Pro Gly Gly Ser Cys Gln Glu Cys 
            980                 985                 990         
Glu Cys Asp Pro Tyr Gly Ser Leu Pro Val Pro Cys Asp Pro Val Thr 
        995                 1000                1005            
Gly Phe Cys Thr Cys Arg Pro Gly Ala Thr Gly Arg Lys Cys Asp Gly 
    1010                1015                1020                
Cys Lys His Trp His Ala Arg Glu Gly Trp Glu Cys Val Phe Cys Gly 
1025                1030                1035                1040
Asp Glu Cys Thr Gly Leu Leu Leu Gly Asp Leu Ala Arg Leu Glu Gln 
                1045                1050                1055    
Met Val Met Ser Ile Asn Leu Thr Gly Pro Leu Pro Ala Pro Tyr Lys 
            1060                1065                1070        
Met Leu Tyr Gly Leu Glu Asn Met Thr Gln Glu Leu Lys His Leu Leu 
        1075                1080                1085            
Ser Pro Gln Arg Ala Pro Glu Arg Leu Ile Gln Leu Ala Glu Gly Asn 
    1090                1095                1100                
Leu Asn Thr Leu Val Thr Glu Met Asn Glu Leu Leu Thr Arg Ala Thr 
1105                1110                1115                1120
Lys Val Thr Ala Asp Gly Glu Gln Thr Gly Gln Asp Ala Glu Arg Thr 
                1125                1130                1135    
Asn Thr Arg Ala Lys Ser Leu Gly Glu Phe Ile Lys Glu Leu Ala Arg 
            1140                1145                1150        
Asp Ala Glu Ala Val Asn Glu Lys Ala Ile Lys Leu Asn Glu Thr Leu 
        1155                1160                1165            
Gly Thr Arg Asp Glu Ala Phe Glu Arg Asn Leu Glu Gly Leu Gln Lys 
    1170                1175                1180                
Glu Ile Asp Gln Met Ile Lys Glu Leu Arg Arg Lys Asn Leu Glu Thr 
1185                1190                1195                1200
Gln Lys Glu Ile Ala Glu Asp Glu Leu Val Ala Ala Glu Ala Leu Leu 
                1205                1210                1215    
Lys Lys Val Lys Lys Leu Phe Gly Glu Ser Arg Gly Glu Asn Glu Glu 
            1220                1225                1230        
Met Glu Lys Asp Leu Arg Glu Lys Leu Ala Asp Tyr Lys Asn Lys Val 
        1235                1240                1245            
Asp Asp Ala Trp Asp Leu Leu Arg Glu Ala Thr Asp Lys Ile Arg Glu 
    1250                1255                1260                
Ala Asn Arg Leu Phe Ala Val Asn Gln Lys Asn Met Thr Ala Leu Glu 
1265                1270                1275                1280
Lys Lys Lys Glu Ala Val Glu Ser Gly Lys Arg Gln Ile Glu Asn Thr 
                1285                1290                1295    
Leu Lys Glu Gly Asn Asp Ile Leu Asp Glu Ala Asn Arg Leu Ala Asp 
            1300                1305                1310        
Glu Ile Asn Ser Ile Ile Asp Tyr Val Glu Asp Ile Gln Thr Lys Leu 
        1315                1320                1325            
Pro Pro Met Ser Glu Glu Leu Asn Asp Lys Ile Asp Asp Leu Ser Gln 
    1330                1335                1340                
Glu Ile Lys Asp Arg Lys Leu Ala Glu Lys Val Ser Gln Ala Glu Ser 
1345                1350                1355                1360
His Ala Ala Gln Leu Asn Asp Ser Ser Ala Val Leu Asp Gly Ile Leu 
                1365                1370                1375    
Asp Glu Ala Lys Asn Ile Ser Phe Asn Ala Thr Ala Ala Phe Lys Ala 
            1380                1385                1390        
Tyr Ser Asn Ile Lys Asp Tyr Ile Asp Glu Ala Glu Lys Val Ala Lys 
        1395                1400                1405            
Glu Ala Lys Asp Leu Ala His Glu Ala Thr Lys Leu Ala Thr Gly Pro 
    1410                1415                1420                
Arg Gly Leu Leu Lys Glu Asp Ala Lys Gly Cys Leu Gln Lys Ser Phe 
1425                1430                1435                1440
Arg Ile Leu Asn Glu Ala Lys Lys Leu Ala Asn Asp Val Lys Glu Asn 
                1445                1450                1455    
Glu Asp His Leu Asn Gly Leu Lys Thr Arg Ile Glu Asn Ala Asp Ala 
            1460                1465                1470        
Arg Asn Gly Asp Leu Leu Arg Thr Leu Asn Asp Thr Leu Gly Lys Leu 
        1475                1480                1485            
Ser Ala Ile Pro Asn Asp Thr Ala Ala Lys Leu Gln Ala Val Lys Asp 
    1490                1495                1500                
Lys Ala Arg Gln Ala Asn Asp Thr Ala Lys Asp Val Leu Ala Gln Ile 
1505                1510                1515                1520
Thr Glu Leu His Gln Asn Leu Asp Gly Leu Lys Lys Asn Tyr Asn Lys 
                1525                1530                1535    
Leu Ala Asp Ser Val Ala Lys Thr Asn Ala Val Val Lys Asp Pro Ser 
            1540                1545                1550        
Lys Asn Lys Ile Ile Ala Asp Ala Asp Ala Thr Val Lys Asn Leu Glu 
        1555                1560                1565            
Gln Glu Ala Asp Arg Leu Ile Asp Lys Leu Lys Pro Ile Lys Glu Leu 
    1570                1575                1580                
Glu Asp Asn Leu Lys Lys Asn Ile Ser Glu Ile Lys Glu Leu Ile Asn 
1585                1590                1595                1600
Gln Ala Arg Lys Gln Ala Asn Ser Ile Lys Val Ser Val Ser Ser Gly 
                1605                1610                1615    
Gly Asp Cys Ile Arg Thr Tyr Lys Pro Glu Ile Lys Lys Gly Ser Tyr 
            1620                1625                1630        
Asn Asn Ile Val Val Asn Val Lys Thr Ala Val Ala Asp Asn Leu Leu 
        1635                1640                1645            
Phe Tyr Leu Gly Ser Ala Lys Phe Ile Asp Phe Leu Ala Ile Glu Met 
    1650                1655                1660                
Arg Lys Gly Lys Val Ser Phe Leu Trp Asp Val Gly Ser Gly Val Gly 
1665                1670                1675                1680
Arg Val Glu Tyr Pro Asp Leu Thr Ile Asp Asp Ser Tyr Trp Tyr Arg 
                1685                1690                1695    
Ile Val Ala Ser Arg Thr Gly Arg Asn Gly Thr Ile Ser Val Arg Ala 
            1700                1705                1710        
Leu Asp Gly Pro Lys Ala Ser Ile Val Pro Ser Thr His His Ser Thr 
        1715                1720                1725            
Ser Pro Pro Gly Tyr Thr Ile Leu Asp Val Asp Ala Asn Ala Met Leu 
    1730                1735                1740                
Phe Val Gly Gly Leu Thr Gly Lys Leu Lys Lys Ala Asp Ala Val Arg 
1745                1750                1755                1760
Val Ile Thr Phe Thr Gly Cys Met Gly Glu Thr Tyr Phe Asp Asn Lys 
                1765                1770                1775    
Pro Ile Gly Leu Trp Asn Phe Arg Glu Lys Glu Gly Asp Cys Lys Gly 
            1780                1785                1790        
Cys Thr Val Ser Pro Gln Val Glu Asp Ser Glu Gly Thr Ile Gln Phe 
        1795                1800                1805            
Asp Gly Glu Gly Tyr Ala Leu Val Ser Arg Pro Ile Arg Trp Tyr Pro 
    1810                1815                1820                
Asn Ile Ser Thr Val Met Phe Lys Phe Arg Thr Phe Ser Ser Ser Ala 
1825                1830                1835                1840
Leu Leu Met Tyr Leu Ala Thr Arg Asp Leu Arg Asp Phe Met Ser Val 
                1845                1850                1855    
Glu Leu Thr Asp Gly His Ile Lys Val Ser Tyr Asp Leu Gly Ser Gly 
            1860                1865                1870        
Met Ala Ser Val Val Ser Asn Gln Asn His Asn Asp Gly Lys Trp Lys 
        1875                1880                1885            
Ser Phe Thr Leu Ser Arg Ile Gln Lys Gln Ala Asn Ile Ser Ile Val 
    1890                1895                1900                
Asp Ile Asp Thr Asn Gln Glu Glu Asn Ile Ala Thr Ser Ser Ser Gly 
1905                1910                1915                1920
Asn Asn Phe Gly Leu Asp Leu Lys Ala Asp Asp Lys Ile Tyr Phe Gly 
                1925                1930                1935    
Gly Leu Pro Thr Leu Arg Asn Leu Ser Met Lys Ala Arg Pro Glu Val 
            1940                1945                1950        
Asn Leu Lys Lys Tyr Ser Gly Cys Leu Lys Asp Ile Glu Ile Ser Arg 
        1955                1960                1965            
Thr Pro Tyr Asn Ile Leu Ser Ser Pro Asp Tyr Val Gly Val Thr Lys 
    1970                1975                1980                
Gly Cys Ser Leu Glu Asn Val Tyr Thr Val Ser Phe Pro Lys Pro Gly 
1985                1990                1995                2000
Phe Val Glu Leu Ser Pro Val Pro Ile Asp Val Gly Thr Glu Ile Asn 
                2005                2010                2015    
Leu Ser Phe Ser Thr Lys Asn Glu Ser Gly Ile Ile Leu Leu Gly Ser 
            2020                2025                2030        
Gly Gly Thr Pro Ala Pro Pro Arg Arg Lys Arg Arg Gln Thr Gly Gln 
        2035                2040                2045            
Ala Tyr Tyr Ala Ile Leu Leu Asn Arg Gly Arg Leu Glu Val His Leu 
    2050                2055                2060                
Ser Thr Gly Ala Arg Thr Met Arg Lys Ile Val Ile Arg Pro Glu Pro 
2065                2070                2075                2080
Asn Leu Phe His Asp Gly Arg Glu His Ser Val His Val Glu Arg Thr 
                2085                2090                2095    
Arg Gly Ile Phe Thr Val Gln Val Asp Glu Asn Arg Arg Tyr Met Gln 
            2100                2105                2110        
Asn Leu Thr Val Glu Gln Pro Ile Glu Val Lys Lys Leu Phe Val Gly 
        2115                2120                2125            
Gly Ala Pro Pro Glu Phe Gln Pro Ser Pro Leu Arg Asn Ile Pro Pro 
    2130                2135                2140                
Phe Glu Gly Cys Ile Trp Asn Leu Val Ile Asn Ser Val Pro Met Asp 
2145                2150                2155                2160
Phe Ala Arg Pro Val Ser Phe Lys Asn Ala Asp Ile Gly Arg Cys Ala 
                2165                2170                2175    
His Gln Lys Leu Arg Glu Asp Glu Asp Gly Ala Ala Pro Ala Glu Ile 
            2180                2185                2190        
Val Ile Gln Pro Glu Pro Val Pro Thr Pro Ala Phe Pro Thr Pro Thr 
        2195                2200                2205            
Pro Val Leu Thr His Gly Pro Cys Ala Ala Glu Ser Glu Pro Ala Leu 
    2210                2215                2220                
Leu Ile Gly Ser Lys Gln Phe Gly Leu Ser Arg Asn Ser His Ile Ala 
2225                2230                2235                2240
Ile Ala Phe Asp Asp Thr Lys Val Lys Asn Arg Leu Thr Ile Glu Leu 
                2245                2250                2255    
Glu Val Arg Thr Glu Ala Glu Ser Gly Leu Leu Phe Tyr Met Ala Arg 
            2260                2265                2270        
Ile Asn His Ala Asp Phe Ala Thr Val Gln Leu Arg Asn Gly Leu Pro 
        2275                2280                2285            
Tyr Phe Ser Tyr Asp Leu Gly Ser Gly Asp Thr His Thr Met Ile Pro 
    2290                2295                2300                
Thr Lys Ile Asn Asp Gly Gln Trp His Lys Ile Lys Ile Met Arg Ser 
2305                2310                2315                2320
Lys Gln Glu Gly Ile Leu Tyr Val Asp Gly Ala Ser Asn Arg Thr Ile 
                2325                2330                2335    
Ser Pro Lys Lys Ala Asp Ile Leu Asp Val Val Gly Met Leu Tyr Val 
            2340                2345                2350        
Gly Gly Leu Pro Ile Asn Tyr Thr Thr Arg Arg Ile Gly Pro Val Thr 
        2355                2360                2365            
Tyr Ser Ile Asp Gly Cys Val Arg Asn Leu His Met Ala Glu Ala Pro 
    2370                2375                2380                
Ala Asp Leu Glu Gln Pro Thr Ser Ser Phe His Val Gly Thr Cys Phe 
2385                2390                2395                2400
Ala Asn Ala Gln Arg Gly Thr Tyr Phe Asp Gly Thr Gly Phe Ala Lys 
                2405                2410                2415    
Ala Val Gly Gly Phe Lys Val Gly Leu Asp Leu Leu Val Glu Phe Glu 
            2420                2425                2430        
Phe Arg Thr Thr Thr Thr Thr Gly Val Leu Leu Gly Ile Ser Ser Gln 
        2435                2440                2445            
Lys Met Asp Gly Met Gly Ile Glu Met Ile Asp Glu Lys Leu Met Phe 
    2450                2455                2460                
His Val Asp Asn Gly Ala Gly Arg Phe Thr Ala Val Tyr Asp Ala Gly 
2465                2470                2475                2480
Val Pro Gly His Leu Cys Asp Gly Gln Trp His Lys Val Thr Ala Asn 
                2485                2490                2495    
Lys Ile Lys His Arg Ile Glu Leu Thr Val Asp Gly Asn Gln Val Glu 
            2500                2505                2510        
Ala Gln Ser Pro Asn Pro Ala Ser Thr Ser Ala Asp Thr Asn Asp Pro 
        2515                2520                2525            
Val Phe Val Gly Gly Phe Pro Asp Asp Leu Lys Gln Phe Gly Leu Thr 
    2530                2535                2540                
Thr Ser Ile Pro Phe Arg Gly Cys Ile Arg Ser Leu Lys Leu Thr Lys 
2545                2550                2555                2560
Gly Thr Gly Lys Pro Leu Glu Val Asn Phe Ala Lys Ala Leu Glu Leu 
                2565                2570                2575    
Arg Gly Val Gln Pro Val Ser Cys Pro Ala Asn 
            2580                2585        

<210> 121
<211> 1916
<212> PRT
<213> Artificial Sequence


<220> 
<223> laminin subunit α2 isoform X6

<400> 121
Met Pro Gly Ala Ala Gly Val Leu Leu Leu Leu Leu Leu Ser Gly Gly 
1               5                   10                  15      
Leu Gly Gly Val Gln Ala Gln Arg Pro Gln Gln Gln Arg Gln Ser Gln 
            20                  25                  30          
Ala His Gln Gln Arg Gly Leu Phe Pro Ala Val Leu Asn Leu Ala Ser 
        35                  40                  45              
Asn Ala Leu Ile Thr Thr Asn Ala Thr Cys Gly Glu Lys Gly Pro Glu 
    50                  55                  60                  
Met Tyr Cys Lys Leu Val Glu His Val Pro Gly Gln Pro Val Arg Asn 
65                  70                  75                  80  
Pro Gln Cys Arg Ile Cys Asn Gln Asn Ser Ser Asn Pro Asn Gln Arg 
                85                  90                  95      
His Pro Ile Thr Asn Ala Ile Asp Gly Lys Asn Thr Trp Trp Gln Ser 
            100                 105                 110         
Pro Ser Ile Lys Asn Gly Ile Glu Tyr His Tyr Val Thr Ile Thr Leu 
        115                 120                 125             
Asp Leu Gln Gln Val Phe Gln Ile Ala Tyr Val Ile Val Lys Ala Ala 
    130                 135                 140                 
Asn Ser Pro Arg Pro Gly Asn Trp Ile Leu Glu Arg Ser Leu Asp Asp 
145                 150                 155                 160 
Val Glu Tyr Lys Pro Trp Gln Tyr His Ala Val Thr Asp Thr Glu Cys 
                165                 170                 175     
Leu Thr Leu Tyr Asn Ile Tyr Pro Arg Thr Gly Pro Pro Ser Tyr Ala 
            180                 185                 190         
Lys Asp Asp Glu Val Ile Cys Thr Ser Phe Tyr Ser Lys Ile His Pro 
        195                 200                 205             
Leu Glu Asn Gly Glu Ile His Ile Ser Leu Ile Asn Gly Arg Pro Ser 
    210                 215                 220                 
Ala Asp Asp Pro Ser Pro Glu Leu Leu Glu Phe Thr Ser Ala Arg Tyr 
225                 230                 235                 240 
Ile Arg Leu Arg Phe Gln Arg Ile Arg Thr Leu Asn Ala Asp Leu Met 
                245                 250                 255     
Met Phe Ala His Lys Asp Pro Arg Glu Ile Asp Pro Ile Val Thr Arg 
            260                 265                 270         
Arg Tyr Tyr Tyr Ser Val Lys Asp Ile Ser Val Gly Gly Met Cys Ile 
        275                 280                 285             
Cys Tyr Gly His Ala Arg Ala Cys Pro Leu Asp Pro Ala Thr Asn Lys 
    290                 295                 300                 
Ser Arg Cys Glu Cys Glu His Asn Thr Cys Gly Asp Ser Cys Asp Gln 
305                 310                 315                 320 
Cys Cys Pro Gly Phe His Gln Lys Pro Trp Arg Ala Gly Thr Phe Leu 
                325                 330                 335     
Thr Lys Thr Glu Cys Glu Ala Cys Asn Cys His Gly Lys Ala Glu Glu 
            340                 345                 350         
Cys Tyr Tyr Asp Glu Asn Val Ala Arg Arg Asn Leu Ser Leu Asn Ile 
        355                 360                 365             
Arg Gly Lys Tyr Ile Gly Gly Gly Val Cys Ile Asn Cys Thr Gln Asn 
    370                 375                 380                 
Thr Ala Gly Ile Asn Cys Glu Thr Cys Thr Asp Gly Phe Phe Arg Pro 
385                 390                 395                 400 
Lys Gly Val Ser Pro Asn Tyr Pro Arg Pro Cys Gln Pro Cys His Cys 
                405                 410                 415     
Asp Pro Ile Gly Ser Leu Asn Glu Val Cys Val Lys Asp Glu Lys His 
            420                 425                 430         
Ala Arg Arg Gly Leu Ala Pro Gly Ser Cys His Cys Lys Thr Gly Phe 
        435                 440                 445             
Gly Gly Val Ser Cys Asp Arg Cys Ala Arg Gly Tyr Thr Gly Tyr Pro 
    450                 455                 460                 
Asp Cys Lys Ala Cys Asn Cys Ser Gly Leu Gly Ser Lys Asn Glu Asp 
465                 470                 475                 480 
Pro Cys Phe Gly Pro Cys Ile Cys Lys Glu Asn Val Glu Gly Gly Asp 
                485                 490                 495     
Cys Ser Arg Cys Lys Ser Gly Phe Phe Asn Leu Gln Glu Asp Asn Trp 
            500                 505                 510         
Lys Gly Cys Asp Glu Cys Phe Cys Ser Gly Val Ser Asn Arg Cys Gln 
        515                 520                 525             
Ser Ser Tyr Trp Thr Tyr Gly Lys Ile Gln Asp Met Ser Gly Trp Tyr 
    530                 535                 540                 
Leu Thr Asp Leu Pro Gly Arg Ile Arg Val Ala Pro Gln Gln Asp Asp 
545                 550                 555                 560 
Leu Asp Ser Pro Gln Gln Ile Ser Ile Ser Asn Ala Glu Ala Arg Gln 
                565                 570                 575     
Ala Leu Pro His Ser Tyr Tyr Trp Ser Ala Pro Ala Pro Tyr Leu Gly 
            580                 585                 590         
Asn Lys Leu Pro Ala Val Gly Gly Gln Leu Thr Phe Thr Ile Ser Tyr 
        595                 600                 605             
Asp Leu Glu Glu Glu Glu Glu Asp Thr Glu Arg Val Leu Gln Leu Met 
    610                 615                 620                 
Ile Ile Leu Glu Gly Asn Asp Leu Ser Ile Ser Thr Ala Gln Asp Glu 
625                 630                 635                 640 
Val Tyr Leu His Pro Ser Glu Glu His Thr Asn Val Leu Leu Leu Lys 
                645                 650                 655     
Glu Glu Ser Phe Thr Ile His Gly Thr His Phe Pro Val Arg Arg Lys 
            660                 665                 670         
Glu Phe Met Thr Val Leu Ala Asn Leu Lys Arg Val Leu Leu Gln Ile 
        675                 680                 685             
Thr Tyr Ser Phe Gly Met Asp Ala Ile Phe Arg Leu Ser Ser Val Asn 
    690                 695                 700                 
Leu Glu Ser Ala Val Ser Tyr Pro Thr Asp Gly Ser Ile Ala Ala Ala 
705                 710                 715                 720 
Val Glu Val Cys Gln Cys Pro Pro Gly Tyr Thr Gly Ser Ser Cys Glu 
                725                 730                 735     
Ser Cys Trp Pro Arg His Arg Arg Val Asn Gly Thr Ile Phe Gly Gly 
            740                 745                 750         
Ile Cys Glu Pro Cys Gln Cys Phe Gly His Ala Glu Ser Cys Asp Asp 
        755                 760                 765             
Val Thr Gly Glu Cys Leu Asn Cys Lys Asp His Thr Gly Gly Pro Tyr 
    770                 775                 780                 
Cys Asp Lys Cys Leu Pro Gly Phe Tyr Gly Glu Pro Thr Lys Gly Thr 
785                 790                 795                 800 
Ser Glu Asp Cys Gln Pro Cys Ala Cys Pro Leu Asn Ile Pro Ser Asn 
                805                 810                 815     
Asn Phe Ser Pro Thr Cys His Leu Asp Arg Ser Leu Gly Leu Ile Cys 
            820                 825                 830         
Asp Gly Cys Pro Val Gly Tyr Thr Gly Pro Arg Cys Glu Arg Cys Ala 
        835                 840                 845             
Glu Gly Tyr Phe Gly Gln Pro Ser Val Pro Gly Gly Ser Cys Gln Pro 
    850                 855                 860                 
Cys Gln Cys Asn Asp Asn Leu Asp Phe Ser Ile Pro Gly Ser Cys Asp 
865                 870                 875                 880 
Ser Leu Ser Gly Ser Cys Leu Ile Cys Lys Pro Gly Thr Thr Gly Arg 
                885                 890                 895     
Tyr Cys Glu Leu Cys Ala Asp Gly Tyr Phe Gly Asp Ala Val Asp Ala 
            900                 905                 910         
Lys Asn Cys Gln Pro Cys Arg Cys Asn Ala Gly Gly Ser Phe Ser Glu 
        915                 920                 925             
Val Cys His Ser Gln Thr Gly Gln Cys Glu Cys Arg Ala Asn Val Gln 
    930                 935                 940                 
Gly Gln Arg Cys Asp Lys Cys Lys Pro Asn Met Trp Arg Asp Pro Glu 
945                 950                 955                 960 
Lys Arg Phe Cys Val Leu Cys Asp Cys Asp Pro Val Gly Ser Val Ser 
                965                 970                 975     
Pro Gln Cys Asp Ile Thr Gly Arg Cys Val Cys Lys Ser Gly Phe Val 
            980                 985                 990         
Gly Lys Gln Cys Asn Leu Gly Arg Gln Val His Gln Gln Glu Glu Gln 
        995                 1000                1005            
Pro Arg Arg Ala Gln Arg Val Leu Gly Ser Pro Gln Arg Trp Ala Ile 
    1010                1015                1020                
Gly Ser Ser Ser Gly Cys Pro Arg Gly Ala Tyr Arg Ala Pro Ala Pro 
1025                1030                1035                1040
Ala Gly Thr Phe Gly Leu Gln Ser Ala Arg Gly Cys Val Pro Cys Asn 
                1045                1050                1055    
Cys Asn Ser Phe Gly Ser Lys Ser Phe Asp Cys Glu Glu Ser Gly Gln 
            1060                1065                1070        
Cys Trp Cys Gln Pro Gly Val Thr Gly Lys Lys Cys Asp Arg Cys Ala 
        1075                1080                1085            
His Gly Tyr Phe Asn Phe Gln Glu Gly Gly Cys Thr Ala Cys Glu Cys 
    1090                1095                1100                
Ser His Leu Gly Asn Asn Cys Asp Pro Lys Thr Gly Arg Cys Ile Cys 
1105                1110                1115                1120
Pro Pro Asn Thr Ile Gly Glu Lys Cys Ser Lys Cys Ala Pro Asn Thr 
                1125                1130                1135    
Trp Gly His Ser Ile Thr Thr Gly Cys Lys Ala Cys Asn Cys Ser Thr 
            1140                1145                1150        
Val Gly Ser Leu Asp Phe Gln Cys Asn Val Asn Thr Gly Gln Cys Asn 
        1155                1160                1165            
Cys His Pro Lys Phe Ser Gly Ala Lys Cys Thr Glu Cys Ser Arg Gly 
    1170                1175                1180                
His Trp Asn Tyr Pro Arg Cys Asn Leu Cys Asp Cys Phe Leu Pro Gly 
1185                1190                1195                1200
Thr Asp Ala Thr Thr Cys Asp Ser Glu Thr Lys Lys Cys Ser Cys Ser 
                1205                1210                1215    
Asp Gln Thr Gly Gln Cys Thr Cys Lys Val Asn Val Glu Gly Ile His 
            1220                1225                1230        
Cys Asp Arg Cys Arg Pro Gly Lys Phe Gly Leu Asp Ala Lys Asn Pro 
        1235                1240                1245            
Leu Gly Cys Ser Ser Cys Tyr Cys Phe Gly Thr Thr Thr Gln Cys Ser 
    1250                1255                1260                
Glu Ala Lys Gly Leu Ile Arg Thr Trp Val Thr Leu Lys Ala Glu Gln 
1265                1270                1275                1280
Thr Ile Leu Pro Leu Val Asp Glu Ala Leu Gln His Thr Thr Thr Lys 
                1285                1290                1295    
Gly Ile Val Phe Gln His Pro Glu Ile Val Ala His Met Asp Leu Met 
            1300                1305                1310        
Arg Glu Asp Leu His Leu Glu Pro Phe Tyr Trp Lys Leu Pro Glu Gln 
        1315                1320                1325            
Phe Glu Gly Lys Lys Leu Met Ala Tyr Gly Gly Lys Leu Lys Tyr Ala 
    1330                1335                1340                
Ile Tyr Phe Glu Ala Arg Glu Glu Thr Gly Phe Ser Thr Tyr Asn Pro 
1345                1350                1355                1360
Gln Val Ile Ile Arg Gly Gly Thr Pro Thr His Ala Arg Ile Ile Val 
                1365                1370                1375    
Arg His Met Ala Ala Pro Leu Ile Gly Gln Leu Thr Arg His Glu Ile 
            1380                1385                1390        
Glu Met Thr Glu Lys Glu Trp Lys Tyr Tyr Gly Asp Asp Pro Arg Val 
        1395                1400                1405            
His Arg Thr Val Thr Arg Glu Asp Phe Leu Asp Ile Leu Tyr Asp Ile 
    1410                1415                1420                
His Tyr Ile Leu Ile Lys Ala Thr Tyr Gly Asn Phe Met Arg Gln Ser 
1425                1430                1435                1440
Arg Ile Ser Glu Ile Ser Met Glu Val Ala Glu Gln Gly Arg Gly Thr 
                1445                1450                1455    
Thr Met Thr Pro Pro Ala Asp Leu Ile Glu Lys Cys Asp Cys Pro Leu 
            1460                1465                1470        
Gly Tyr Ser Gly Leu Ser Cys Glu Ala Cys Leu Pro Gly Phe Tyr Arg 
        1475                1480                1485            
Leu Arg Ser Gln Pro Gly Gly Arg Thr Pro Gly Pro Thr Leu Gly Thr 
    1490                1495                1500                
Cys Val Pro Cys Gln Cys Asn Gly His Ser Ser Leu Cys Asp Pro Glu 
1505                1510                1515                1520
Thr Ser Ile Cys Gln Asn Cys Gln His His Thr Ala Gly Asp Phe Cys 
                1525                1530                1535    
Glu Arg Cys Ala Leu Gly Tyr Tyr Gly Ile Val Lys Gly Leu Pro Asn 
            1540                1545                1550        
Asp Cys Gln Gln Cys Ala Cys Pro Leu Ile Ser Ser Ser Asn Asn Phe 
        1555                1560                1565            
Ser Pro Ser Cys Val Ala Glu Gly Leu Asp Asp Tyr Arg Cys Thr Ala 
    1570                1575                1580                
Cys Pro Arg Gly Tyr Glu Gly Gln Tyr Cys Glu Arg Cys Ala Pro Gly 
1585                1590                1595                1600
Tyr Thr Gly Ser Pro Gly Asn Pro Gly Gly Ser Cys Gln Glu Cys Glu 
                1605                1610                1615    
Cys Asp Pro Tyr Gly Ser Leu Pro Val Pro Cys Asp Pro Val Thr Gly 
            1620                1625                1630        
Phe Cys Thr Cys Arg Pro Gly Ala Thr Gly Arg Lys Cys Asp Gly Cys 
        1635                1640                1645            
Lys His Trp His Ala Arg Glu Gly Trp Glu Cys Val Phe Cys Gly Asp 
    1650                1655                1660                
Glu Cys Thr Gly Leu Leu Leu Gly Asp Leu Ala Arg Leu Glu Gln Met 
1665                1670                1675                1680
Val Met Ser Ile Asn Leu Thr Gly Pro Leu Pro Ala Pro Tyr Lys Met 
                1685                1690                1695    
Leu Tyr Gly Leu Glu Asn Met Thr Gln Glu Leu Lys His Leu Leu Ser 
            1700                1705                1710        
Pro Gln Arg Ala Pro Glu Arg Leu Ile Gln Leu Ala Glu Gly Asn Leu 
        1715                1720                1725            
Asn Thr Leu Val Thr Glu Met Asn Glu Leu Leu Thr Arg Ala Thr Lys 
    1730                1735                1740                
Val Thr Ala Asp Gly Glu Gln Thr Gly Gln Asp Ala Glu Arg Thr Asn 
1745                1750                1755                1760
Thr Arg Ala Lys Ser Leu Gly Glu Phe Ile Lys Glu Leu Ala Arg Asp 
                1765                1770                1775    
Ala Glu Ala Val Asn Glu Lys Ala Ile Lys Leu Asn Glu Thr Leu Gly 
            1780                1785                1790        
Thr Arg Asp Glu Ala Phe Glu Arg Asn Leu Glu Gly Leu Gln Lys Glu 
        1795                1800                1805            
Ile Asp Gln Met Ile Lys Glu Leu Arg Arg Lys Asn Leu Glu Thr Gln 
    1810                1815                1820                
Lys Glu Ile Ala Glu Asp Glu Leu Val Ala Ala Glu Ala Leu Leu Lys 
1825                1830                1835                1840
Lys Val Lys Lys Leu Phe Gly Glu Ser Arg Gly Glu Asn Glu Glu Met 
                1845                1850                1855    
Glu Lys Asp Leu Arg Glu Lys Leu Ala Asp Tyr Lys Asn Lys Val Asp 
            1860                1865                1870        
Asp Ala Trp Asp Leu Leu Arg Glu Ala Thr Asp Lys Ile Arg Glu Ala 
        1875                1880                1885            
Asn Arg Leu Phe Ala Val Asn Gln Lys Asn Met Thr Ala Leu Glu Gln 
    1890                1895                1900                
Leu Pro Ala Lys Val Ile Lys Thr Asn Gln Ser Ile 
1905                1910                1915    

<210> 122
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified Hyperactive Piggybac triple mutant
      D450N/R372A/K375A/R376A

<400> 122
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Arg Gly Arg Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Ala Ser Asn Ala Ala Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asn Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560 
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Ser Ala Ser Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser 
            580                 585                 590         
Cys Phe 
        

<210> 123
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac
      R245A/N347S/R372A/D450N/T560A/S564P/S573A/S592G

<400> 123
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Ala Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Arg Gly Arg Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Ser Trp Phe Thr Ser Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Ala Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asn Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Ala 
545                 550                 555                 560 
Tyr Cys Pro Pro Lys Ile Arg Arg Lys Ala Ser Ala Ala Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Gly 
            580                 585                 590         
Cys Phe 
        

<210> 124
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac
      R277A/G325A/N347A/K375A/D450N/T560A/S564P/S573A/S592G/F594L

<400> 124
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Arg Gly Ala Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Ala Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Ala Trp Phe Thr Ser Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Arg Ser Asn Ala Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asn Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Ala 
545                 550                 555                 560 
Tyr Cys Pro Pro Lys Ile Arg Arg Lys Ala Ser Ala Ala Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Gly 
            580                 585                 590         
Cys Leu 
        

<210> 125
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac
      V34M/R275A/G325A/N347S/S351A/R372A/K375A/D450N/T560A/S564P

<400> 125
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Met Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Ala Gly Arg Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Ala Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Ser Trp Phe Thr Ala Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Ala Ser Asn Ala Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asn Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Ala 
545                 550                 555                 560 
Tyr Cys Pro Pro Lys Ile Arg Arg Lys Ala Ser Ala Ser Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser 
            580                 585                 590         
Cys Phe 
        

<210> 126
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac
      G325A/N347S/K375A/D450N/S573A/M589V/S592G

<400> 126
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Arg Gly Arg Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Ala Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Ser Trp Phe Thr Ser Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Arg Ser Asn Ala Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asn Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560 
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Ser Ala Ala Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Val Cys Gln Gly 
            580                 585                 590         
Cys Phe 
        

<210> 127
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac S230N/R277A/N347S/K375A/D450N

<400> 127
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Asn Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Arg Gly Ala Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Ser Trp Phe Thr Ser Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Ala Asn 
    370                 375                 380                 
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asn Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560 
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Ser Ala Ser Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser 
            580                 585                 590         
Cys Phe 
        

<210> 128
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac T43I/R372A/K375A/A411T/D450N

<400> 128
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Ile Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Arg Gly Arg Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Ala Ser Asn Ala Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Thr Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asn Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560 
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Ser Ala Ser Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser 
            580                 585                 590         
Cys Phe 
        

<210> 129
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac
      G325A/N347S/S351A/K375A/D450N/S573A/M589V/S592G

<400> 129
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Arg Gly Arg Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Ala Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Ser Trp Phe Thr Ala Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Arg Ser Asn Ala Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asn Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560 
Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Ser Ala Ala Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Val Cys Gln Gly 
            580                 585                 590         
Cys Phe 
        

<210> 130
<211> 594
<212> PRT
<213> Artificial Sequence


<220> 
<223> Modified hyperactive PiggyBac
      Y177H/R275A/G325A/K375A/D450N/T560A/S564P/S592G

<400> 130
Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      
Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Val Ser Asp 
            20                  25                  30          
His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              
Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  
Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  
Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      
Cys Trp Ser Thr Ser Lys Pro Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         
Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             
Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 
Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 
Glu Ser Met Thr Ser Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     
His Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         
His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             
Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 
Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 
Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     
Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         
Gly Phe Ala Gly Arg Cys Pro Phe Arg Val Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             
Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 
Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 
Gly Val Pro Leu Ala Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     
His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350         
Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             
Gly Thr Val Arg Ser Asn Ala Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 
Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 
Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     
Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         
Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             
Leu Asn Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 
Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 
Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     
Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Gly Leu Thr Ser 
            500                 505                 510         
Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             
Arg Asp Asn Ile Ser Asn Ile Leu Pro Lys Glu Val Pro Gly Thr Ser 
    530                 535                 540                 
Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Ala 
545                 550                 555                 560 
Tyr Cys Pro Gly Lys Ile Arg Arg Lys Ala Ser Ala Ser Cys Lys Lys 
                565                 570                 575     
Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Gly 
            580                 585                 590         
Cys Phe 
        

<210> 131
<211> 390
<212> DNA
<213> Artificial Sequence


<220> 
<223> MCP N55K

<400> 131
atggcttcaa actttactca gttcgtgctc gtggacaatg gtgggacagg ggatgtgaca      60

gtggctcctt ctaatttcgc taatggggtg gcagagtgga tcagctccaa ctcacggagc     120

caggcctaca aggtgacatg cagcgtcagg cagtctagtg cccagaagag aaagtatacc     180

atcaaggtgg aggtccccaa agtggctacc cagacagtgg gcggagtcga actgcctgtc     240

gccgcttgga ggtcctacct gaacatggag ctcactatcc caattttcgc taccaattct     300

gactgtgaac tcatcgtgaa ggcaatgcag gggctcctca aagacggtaa tcctatccct     360

tccgccatcg ccgctaactc aggtatctac                                      390


<210> 132
<211> 130
<212> PRT
<213> Artificial Sequence


<220> 
<223> MCP N55K

<400> 132
Met Ala Ser Asn Phe Thr Gln Phe Val Leu Val Asp Asn Gly Gly Thr 
1               5                   10                  15      
Gly Asp Val Thr Val Ala Pro Ser Asn Phe Ala Asn Gly Val Ala Glu 
            20                  25                  30          
Trp Ile Ser Ser Asn Ser Arg Ser Gln Ala Tyr Lys Val Thr Cys Ser 
        35                  40                  45              
Val Arg Gln Ser Ser Ala Gln Lys Arg Lys Tyr Thr Ile Lys Val Glu 
    50                  55                  60                  
Val Pro Lys Val Ala Thr Gln Thr Val Gly Gly Val Glu Leu Pro Val 
65                  70                  75                  80  
Ala Ala Trp Arg Ser Tyr Leu Asn Met Glu Leu Thr Ile Pro Ile Phe 
                85                  90                  95      
Ala Thr Asn Ser Asp Cys Glu Leu Ile Val Lys Ala Met Gln Gly Leu 
            100                 105                 110         
Leu Lys Asp Gly Asn Pro Ile Pro Ser Ala Ile Ala Ala Asn Ser Gly 
        115                 120                 125             
Ile Tyr 
    130 

<210> 133
<211> 162
<212> DNA
<213> Artificial Sequence


<220> 
<223> gRNA-MS2(tetraloop)-AAVS1-3 spacer

<400> 133
ggggccacta gggacaggat gttttagagc taggccaaca tgaggatcac ccatgtctgc     60

agggcctagc aagttaaaat aaggctagtc cgttatcaac ttggccaaca tgaggatcac    120

ccatgtctgc agggccaagt ggcaccgagt cggtgctttt tt                       162


<210> 134
<211> 162
<212> RNA
<213> Artificial Sequence


<220> 
<223> gRNA-MS2(tetraloop)-AAVS1-3 spacer

<400> 134
ggggccacua gggacaggau guuuuagagc uaggccaaca ugaggaucac ccaugucugc     60

agggccuagc aaguuaaaau aaggcuaguc cguuaucaac uuggccaaca ugaggaucac    120

ccaugucugc agggccaagu ggcaccgagu cggugcuuuu uu                       162


