﻿               SEQUENCE LISTING

<110> MOLECULAR PARTNERS AG

<120> RECOMBINANT CD3 BINDING PROTEINS AND THEIR USE

<130> AD4028 PCT S3

<150> US 63/126,356
<151> 2020-12-16

<150> US 63/182,394
<151> 2021-04-30

<150> EP 20 216 705.2
<151> 2020-12-22

<160> 46

<170> BiSSAP 1.3.6

<210> 1
<211> 124
<212> PRT
<213> Artificial Sequence


<220> 
<223> ankyrin repeat domain specific for CD3

<400> 1
Asp Leu Gly Gln Lys Leu Leu Glu Ala Ala Trp Ala Gly Gln Asp Asp 
1               5                   10                  15      
Glu Val Arg Glu Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp 
            20                  25                  30          
Ser Gln Gly Trp Thr Pro Leu His Thr Ala Ala Gln Thr Gly His Leu 
        35                  40                  45              
Glu Ile Phe Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys 
    50                  55                  60                  
Asp Asp Lys Gly Val Thr Pro Leu His Leu Ala Ala Ala Leu Gly His 
65                  70                  75                  80  
Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala 
                85                  90                  95      
Gln Asp Ser Trp Gly Thr Thr Pro Ala Asp Leu Ala Ala Lys Tyr Gly 
            100                 105                 110         
His Glu Asp Ile Ala Glu Val Leu Gln Lys Ala Ala 
        115                 120                 

<210> 2
<211> 124
<212> PRT
<213> Artificial Sequence


<220> 
<223> Ankyrin repeat domain specific for CD3

<400> 2
Asp Leu Gly Gln Lys Leu Leu Glu Ala Ala Trp Ala Gly Gln Asp Asp 
1               5                   10                  15      
Glu Val Arg Glu Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asn 
            20                  25                  30          
Ser Arg Gly Trp Thr Pro Leu His Thr Ala Ala Gln Thr Gly His Leu 
        35                  40                  45              
Glu Ile Phe Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys 
    50                  55                  60                  
Asp Asp Lys Gly Val Thr Pro Leu His Leu Ala Ala Ala Leu Gly His 
65                  70                  75                  80  
Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala 
                85                  90                  95      
Gln Asp Ser Trp Gly Thr Thr Pro Ala Asp Leu Ala Ala Lys Tyr Gly 
            100                 105                 110         
His Glu Asp Ile Ala Glu Val Leu Gln Lys Ala Ala 
        115                 120                 

<210> 3
<211> 124
<212> PRT
<213> Artificial Sequence


<220> 
<223> Ankyrin repeat domain specific for CD3

<400> 3
Asp Leu Gly Gln Lys Leu Leu Glu Ala Ala Trp Ala Gly Gln Asp Asp 
1               5                   10                  15      
Glu Val Arg Glu Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asn 
            20                  25                  30          
Ser Arg Gly Trp Thr Pro Leu His Thr Ala Ala Gln Thr Gly His Leu 
        35                  40                  45              
Glu Ile Phe Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys 
    50                  55                  60                  
Asn Asp Lys Arg Val Thr Pro Leu His Leu Ala Ala Ala Leu Gly His 
65                  70                  75                  80  
Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala 
                85                  90                  95      
Arg Asp Ser Trp Gly Thr Thr Pro Ala Asp Leu Ala Ala Lys Tyr Gly 
            100                 105                 110         
His Gln Asp Ile Ala Glu Val Leu Gln Lys Ala Ala 
        115                 120                 

<210> 4
<211> 124
<212> PRT
<213> Artificial Sequence


<220> 
<223> Ankyrin repeat domain specific for CD3

<400> 4
Asp Leu Gly Gln Lys Leu Leu Glu Ala Ala Trp Ala Gly Gln Leu Asp 
1               5                   10                  15      
Glu Val Arg Ile Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asn 
            20                  25                  30          
Ser Arg Gly Trp Thr Pro Leu His Thr Ala Ala Gln Thr Gly His Leu 
        35                  40                  45              
Glu Ile Phe Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys 
    50                  55                  60                  
Thr Asn Lys Arg Val Thr Pro Leu His Leu Ala Ala Ala Leu Gly His 
65                  70                  75                  80  
Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala 
                85                  90                  95      
Arg Asp Thr Trp Gly Thr Thr Pro Ala Asp Leu Ala Ala Lys Tyr Gly 
            100                 105                 110         
His Arg Asp Ile Ala Glu Val Leu Gln Lys Ala Ala 
        115                 120                 

<210> 5
<211> 33
<212> PRT
<213> Artificial Sequence


<220> 
<223> Ankyrin repeat module 1

<400> 5
Lys Asp Ser Gln Gly Trp Thr Pro Leu His Thr Ala Ala Gln Thr Gly 
1               5                   10                  15      
His Leu Glu Ile Phe Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn 
            20                  25                  30          
Ala 
    

<210> 6
<211> 33
<212> PRT
<213> Artificial Sequence


<220> 
<223> Ankyrin repeat module 2

<400> 6
Lys Asp Asp Lys Gly Val Thr Pro Leu His Leu Ala Ala Ala Leu Gly 
1               5                   10                  15      
His Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn 
            20                  25                  30          
Ala 
    

<210> 7
<211> 33
<212> PRT
<213> Artificial Sequence


<220> 
<223> Ankyrin repeat module 3

<400> 7
Lys Asn Ser Arg Gly Trp Thr Pro Leu His Thr Ala Ala Gln Thr Gly 
1               5                   10                  15      
His Leu Glu Ile Phe Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn 
            20                  25                  30          
Ala 
    

<210> 8
<211> 33
<212> PRT
<213> Artificial Sequence


<220> 
<223> Ankyrin repeat module 4

<400> 8
Lys Asn Asp Lys Arg Val Thr Pro Leu His Leu Ala Ala Ala Leu Gly 
1               5                   10                  15      
His Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn 
            20                  25                  30          
Ala 
    

<210> 9
<211> 33
<212> PRT
<213> Artificial Sequence


<220> 
<223> Ankyrin repeat module 5

<400> 9
Lys Thr Asn Lys Arg Val Thr Pro Leu His Leu Ala Ala Ala Leu Gly 
1               5                   10                  15      
His Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn 
            20                  25                  30          
Ala 
    

<210> 10
<211> 30
<212> PRT
<213> Artificial Sequence


<220> 
<223> N -capping module 1

<400> 10
Asp Leu Gly Gln Lys Leu Leu Glu Ala Ala Trp Ala Gly Gln Asp Asp 
1               5                   10                  15      
Glu Val Arg Glu Leu Leu Lys Ala Gly Ala Asp Val Asn Ala 
            20                  25                  30  

<210> 11
<211> 30
<212> PRT
<213> Artificial Sequence


<220> 
<223> N -capping module 2

<400> 11
Asp Leu Gly Gln Lys Leu Leu Glu Ala Ala Trp Ala Gly Gln Leu Asp 
1               5                   10                  15      
Glu Val Arg Ile Leu Leu Lys Ala Gly Ala Asp Val Asn Ala 
            20                  25                  30  

<210> 12
<211> 30
<212> PRT
<213> Artificial Sequence


<220> 
<223> N -capping module 3

<400> 12
Asp Leu Gly Gln Lys Leu Leu Glu Ala Ala Trp Ala Gly Gln Asp Asp 
1               5                   10                  15      
Glu Val Arg Ile Leu Leu Lys Ala Gly Ala Asp Val Asn Ala 
            20                  25                  30  

<210> 13
<211> 30
<212> PRT
<213> Artificial Sequence


<220> 
<223> N -capping module 4

<400> 13
Asp Leu Gly Gln Lys Leu Leu Glu Ala Ala Trp Ala Gly Gln Asp Asp 
1               5                   10                  15      
Glu Val Arg Ile Leu Leu Ala Ala Gly Ala Asp Val Asn Ala 
            20                  25                  30  

<210> 14
<211> 30
<212> PRT
<213> Artificial Sequence


<220> 
<223> N -capping module 5

<400> 14
Asp Leu Gly Gln Lys Leu Leu Glu Ala Ala Trp Ala Gly Gln Leu Asp 
1               5                   10                  15      
Glu Val Arg Glu Leu Leu Lys Ala Gly Ala Asp Val Asn Ala 
            20                  25                  30  

<210> 15
<211> 30
<212> PRT
<213> Artificial Sequence


<220> 
<223> N -capping module 6

<400> 15
Asp Leu Gly Gln Lys Leu Leu Glu Ala Ala Trp Ala Gly Gln Leu Asp 
1               5                   10                  15      
Glu Val Arg Ile Leu Leu Ala Ala Gly Ala Asp Val Asn Ala 
            20                  25                  30  

<210> 16
<211> 30
<212> PRT
<213> Artificial Sequence


<220> 
<223> N- capping module randomized

<220> 
<221> VARIANT
<222> 4
<223> xaa can be any naturally occuring amino acid

<220> 
<221> VARIANT
<222> 5
<223> xaa can be any naturally occuring amino acid

<220> 
<221> VARIANT
<222> 11
<223> xaa can be any naturally occuring amino acid

<220> 
<221> VARIANT
<222> 12
<223> xaa can be any naturally occuring amino acid

<220> 
<221> VARIANT
<222> 17
<223> xaa can be any naturally occuring amino acid

<220> 
<221> VARIANT
<222> 20
<223> xaa can be any naturally occuring amino acid

<220> 
<221> VARIANT
<222> 22
<223> xaa can be any naturally occuring amino acid

<220> 
<221> VARIANT
<222> 23
<223> xaa can be any naturally occuring amino acid

<220> 
<221> VARIANT
<222> 24
<223> xaa can be any naturally occuring amino acid

<400> 16
Asp Leu Gly Xaa Xaa Leu Leu Gln Ala Ala Xaa Xaa Gly Gln Leu Asp 
1               5                   10                  15      
Xaa Val Arg Xaa Leu Xaa Xaa Xaa Gly Ala Asp Val Asn Ala 
            20                  25                  30  

<210> 17
<211> 28
<212> PRT
<213> Artificial Sequence


<220> 
<223> C-capping module 1

<400> 17
Gln Asp Ser Trp Gly Thr Thr Pro Ala Asp Leu Ala Ala Lys Tyr Gly 
1               5                   10                  15      
His Glu Asp Ile Ala Glu Val Leu Gln Lys Ala Ala 
            20                  25              

<210> 18
<211> 28
<212> PRT
<213> Artificial Sequence


<220> 
<223> C-capping module 2

<400> 18
Arg Asp Ser Trp Gly Thr Thr Pro Ala Asp Leu Ala Ala Lys Tyr Gly 
1               5                   10                  15      
His Gln Asp Ile Ala Glu Val Leu Gln Lys Ala Ala 
            20                  25              

<210> 19
<211> 28
<212> PRT
<213> Artificial Sequence


<220> 
<223> C-capping module 3

<400> 19
Arg Asp Thr Trp Gly Thr Thr Pro Ala Asp Leu Ala Ala Lys Tyr Gly 
1               5                   10                  15      
His Arg Asp Ile Ala Glu Val Leu Gln Lys Ala Ala 
            20                  25              

<210> 20
<211> 28
<212> PRT
<213> Artificial Sequence


<220> 
<223> C-capping module 4

<400> 20
Gln Asp Ser Trp Gly Thr Thr Pro Ala Asp Leu Ala Ala Lys Tyr Gly 
1               5                   10                  15      
His Glu Asp Ile Ala Glu Val Leu Gln Lys Leu Ala 
            20                  25              

<210> 21
<211> 28
<212> PRT
<213> Artificial Sequence


<220> 
<223> C-capping module 5

<400> 21
Gln Asp Ser Trp Gly Thr Thr Pro Ala Asp Leu Ala Ala Lys Tyr Gly 
1               5                   10                  15      
His Glu Asp Ile Ala Glu Val Leu Gln Lys Leu Asn 
            20                  25              

<210> 22
<211> 28
<212> PRT
<213> Artificial Sequence


<220> 
<223> C-capping module 6

<400> 22
Arg Asp Ser Trp Gly Thr Thr Pro Ala Asp Leu Ala Ala Lys Tyr Gly 
1               5                   10                  15      
His Gln Asp Ile Ala Glu Val Leu Gln Lys Leu Ala 
            20                  25              

<210> 23
<211> 28
<212> PRT
<213> Artificial Sequence


<220> 
<223> C-capping module 7

<400> 23
Arg Asp Ser Trp Gly Thr Thr Pro Ala Asp Leu Ala Ala Lys Tyr Gly 
1               5                   10                  15      
His Gln Asp Ile Ala Glu Val Leu Gln Lys Leu Asn 
            20                  25              

<210> 24
<211> 28
<212> PRT
<213> Artificial Sequence


<220> 
<223> C-capping module 8

<400> 24
Arg Asp Thr Trp Gly Thr Thr Pro Ala Asp Leu Ala Ala Lys Tyr Gly 
1               5                   10                  15      
His Arg Asp Ile Ala Glu Val Leu Gln Lys Leu Ala 
            20                  25              

<210> 25
<211> 28
<212> PRT
<213> Artificial Sequence


<220> 
<223> C-capping module 9

<400> 25
Arg Asp Thr Trp Gly Thr Thr Pro Ala Asp Leu Ala Ala Lys Tyr Gly 
1               5                   10                  15      
His Arg Asp Ile Ala Glu Val Leu Gln Lys Leu Asn 
            20                  25              

<210> 26
<211> 28
<212> PRT
<213> Artificial Sequence


<220> 
<223> C-capping module randomized

<220> 
<221> VARIANT
<222> 1
<223> Xaa can be any naturally occuring amino acid

<220> 
<221> VARIANT
<222> 3
<223> Xaa can be any naturally occuring amino acid

<220> 
<221> VARIANT
<222> 4
<223> Xaa can be any naturally occuring amino acid

<220> 
<221> VARIANT
<222> 6
<223> Xaa can be any naturally occuring amino acid

<220> 
<221> VARIANT
<222> 11
<223> Xaa can be any naturally occuring amino acid

<220> 
<221> VARIANT
<222> 15
<223> Xaa can be any naturally occuring amino acid

<220> 
<221> VARIANT
<222> 19
<223> Xaa can be any naturally occuring amino acid

<220> 
<221> VARIANT
<222> 22
<223> Xaa can be any naturally occuring amino acid

<220> 
<221> VARIANT
<222> 26
<223> Xaa can be any naturally occuring amino acid

<400> 26
Xaa Asp Xaa Xaa Gly Xaa Thr Pro Ala Asp Xaa Ala Ala Arg Xaa Gly 
1               5                   10                  15      
His Gln Xaa Ile Ala Xaa Val Leu Gln Xaa Ala Ala 
            20                  25              

<210> 27
<211> 12
<212> PRT
<213> Artificial Sequence


<220> 
<223> His tag

<400> 27
Met Arg Gly Ser His His His His His His Gly Ser 
1               5                   10          

<210> 28
<211> 124
<212> PRT
<213> Artificial Sequence


<220> 
<223> Ankyrin repeat domain specific for human serum albumin 1

<400> 28
Asp Leu Gly Lys Lys Leu Leu Glu Ala Ala Arg Ala Gly Gln Asp Asp 
1               5                   10                  15      
Glu Val Arg Glu Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp 
            20                  25                  30          
Tyr Phe Ser His Thr Pro Leu His Leu Ala Ala Arg Asn Gly His Leu 
        35                  40                  45              
Lys Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys 
    50                  55                  60                  
Asp Phe Ala Gly Lys Thr Pro Leu His Leu Ala Ala Asn Glu Gly His 
65                  70                  75                  80  
Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala 
                85                  90                  95      
Gln Asp Ile Phe Gly Lys Thr Pro Ala Asp Ile Ala Ala Asp Ala Gly 
            100                 105                 110         
His Glu Asp Ile Ala Glu Val Leu Gln Lys Ala Ala 
        115                 120                 

<210> 29
<211> 124
<212> PRT
<213> Artificial Sequence


<220> 
<223> Ankyrin repeat domain specific for human serum albumin 2

<400> 29
Asp Leu Gly Lys Lys Leu Leu Glu Ala Ala Arg Ala Gly Gln Asp Asp 
1               5                   10                  15      
Glu Val Arg Glu Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp 
            20                  25                  30          
Tyr Phe Ser His Thr Pro Leu His Leu Ala Ala Arg Asn Gly His Leu 
        35                  40                  45              
Lys Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys 
    50                  55                  60                  
Asp Phe Ala Gly Lys Thr Pro Leu His Leu Ala Ala Ala Asp Gly His 
65                  70                  75                  80  
Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala 
                85                  90                  95      
Gln Asp Ile Phe Gly Lys Thr Pro Ala Asp Ile Ala Ala Asp Ala Gly 
            100                 105                 110         
His Glu Asp Ile Ala Glu Val Leu Gln Lys Ala Ala 
        115                 120                 

<210> 30
<211> 124
<212> PRT
<213> Artificial Sequence


<220> 
<223> Ankyrin repeat domain specific for human serum albumin 3

<400> 30
Asp Leu Gly Lys Lys Leu Leu Glu Ala Ala Arg Ala Gly Gln Asp Asp 
1               5                   10                  15      
Glu Val Arg Glu Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp 
            20                  25                  30          
Tyr Phe Ser His Thr Pro Leu His Leu Ala Ala Arg Asn Gly His Leu 
        35                  40                  45              
Lys Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys 
    50                  55                  60                  
Asp Phe Ala Gly Lys Thr Pro Leu His Leu Ala Ala Asp Ala Gly His 
65                  70                  75                  80  
Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala 
                85                  90                  95      
Gln Asp Ile Phe Gly Lys Thr Pro Ala Asp Ile Ala Ala Asp Ala Gly 
            100                 105                 110         
His Glu Asp Ile Ala Glu Val Leu Gln Lys Ala Ala 
        115                 120                 

<210> 31
<211> 22
<212> PRT
<213> Artificial Sequence


<220> 
<223> PT linker

<400> 31
Gly Ser Pro Thr Pro Thr Pro Thr Thr Pro Thr Pro Thr Pro Thr Thr 
1               5                   10                  15      
Pro Thr Pro Thr Pro Thr 
            20          

<210> 32
<211> 222
<212> PRT
<213> Artificial Sequence


<220> 
<223> scCD3ÎµÎ³_Avi-Bio

<400> 32
Met Gln Asp Gly Asn Glu Glu Met Gly Gly Ile Thr Gln Thr Pro Tyr 
1               5                   10                  15      
Lys Val Ser Ile Ser Gly Thr Thr Val Ile Leu Thr Cys Pro Gln Tyr 
            20                  25                  30          
Pro Gly Ser Glu Ile Leu Trp Gln His Asn Asp Lys Asn Ile Gly Gly 
        35                  40                  45              
Asp Glu Asp Asp Lys Asn Ile Gly Ser Asp Glu Asp His Leu Ser Leu 
    50                  55                  60                  
Lys Glu Phe Ser Glu Leu Glu Gln Ser Gly Tyr Tyr Val Cys Tyr Pro 
65                  70                  75                  80  
Arg Gly Ser Lys Pro Glu Asp Ala Asn Phe Tyr Leu Tyr Leu Arg Ala 
                85                  90                  95      
Arg Val Gly Ser Ala Asp Asp Ala Lys Lys Asp Ala Ala Lys Lys Asp 
            100                 105                 110         
Asp Ala Lys Lys Asp Asp Ala Lys Lys Asp Gly Ser Gln Ser Ile Lys 
        115                 120                 125             
Gly Asn His Leu Val Lys Val Tyr Asp Tyr Gln Glu Asp Gly Ser Val 
    130                 135                 140                 
Leu Leu Thr Cys Asp Ala Glu Ala Lys Asn Ile Thr Trp Phe Lys Asp 
145                 150                 155                 160 
Gly Lys Met Ile Gly Phe Leu Thr Glu Asp Lys Lys Lys Trp Asn Leu 
                165                 170                 175     
Gly Ser Asn Ala Lys Asp Pro Arg Gly Met Tyr Gln Cys Lys Gly Ser 
            180                 185                 190         
Gln Asn Lys Ser Lys Pro Leu Gln Val Tyr Tyr Arg Met Gly Gly Gly 
        195                 200                 205             
Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu 
    210                 215                 220         

<210> 33
<211> 272
<212> PRT
<213> Artificial Sequence


<220> 
<223> Ankyrin repeat protein specific for HSA-CD3

<400> 33
Asp Leu Gly Lys Lys Leu Leu Glu Ala Ala Arg Ala Gly Gln Asp Asp 
1               5                   10                  15      
Glu Val Arg Glu Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp 
            20                  25                  30          
Tyr Phe Ser His Thr Pro Leu His Leu Ala Ala Arg Asn Gly His Leu 
        35                  40                  45              
Lys Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys 
    50                  55                  60                  
Asp Phe Ala Gly Lys Thr Pro Leu His Leu Ala Ala Ala Asp Gly His 
65                  70                  75                  80  
Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala 
                85                  90                  95      
Gln Asp Ile Phe Gly Lys Thr Pro Ala Asp Ile Ala Ala Asp Ala Gly 
            100                 105                 110         
His Glu Asp Ile Ala Glu Val Leu Gln Lys Ala Ala Gly Ser Pro Thr 
        115                 120                 125             
Pro Thr Pro Thr Thr Pro Thr Pro Thr Pro Thr Thr Pro Thr Pro Thr 
    130                 135                 140                 
Pro Thr Gly Ser Asp Leu Gly Gln Lys Leu Leu Glu Ala Ala Trp Ala 
145                 150                 155                 160 
Gly Gln Asp Asp Glu Val Arg Glu Leu Leu Lys Ala Gly Ala Asp Val 
                165                 170                 175     
Asn Ala Lys Asp Ser Gln Gly Trp Thr Pro Leu His Thr Ala Ala Gln 
            180                 185                 190         
Thr Gly His Leu Glu Ile Phe Glu Val Leu Leu Lys Ala Gly Ala Asp 
        195                 200                 205             
Val Asn Ala Lys Asp Asp Lys Gly Val Thr Pro Leu His Leu Ala Ala 
    210                 215                 220                 
Ala Leu Gly His Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala 
225                 230                 235                 240 
Asp Val Asn Ala Gln Asp Ser Trp Gly Thr Thr Pro Ala Asp Leu Ala 
                245                 250                 255     
Ala Lys Tyr Gly His Glu Asp Ile Ala Glu Val Leu Gln Lys Ala Ala 
            260                 265                 270         



<210> 34
<211> 272
<212> PRT
<213> Artificial Sequence


<220> 
<223> Ankyrin repeat protein specific for HSA-CD3

<400> 34
Asp Leu Gly Lys Lys Leu Leu Glu Ala Ala Arg Ala Gly Gln Asp Asp 
1               5                   10                  15      
Glu Val Arg Glu Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp 
            20                  25                  30          
Tyr Phe Ser His Thr Pro Leu His Leu Ala Ala Arg Asn Gly His Leu 
        35                  40                  45              
Lys Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys 
    50                  55                  60                  
Asp Phe Ala Gly Lys Thr Pro Leu His Leu Ala Ala Ala Asp Gly His 
65                  70                  75                  80  
Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala 
                85                  90                  95      
Gln Asp Ile Phe Gly Lys Thr Pro Ala Asp Ile Ala Ala Asp Ala Gly 
            100                 105                 110         
His Glu Asp Ile Ala Glu Val Leu Gln Lys Ala Ala Gly Ser Pro Thr 
        115                 120                 125             
Pro Thr Pro Thr Thr Pro Thr Pro Thr Pro Thr Thr Pro Thr Pro Thr 
    130                 135                 140                 
Pro Thr Gly Ser Asp Leu Gly Gln Lys Leu Leu Glu Ala Ala Trp Ala 
145                 150                 155                 160 
Gly Gln Asp Asp Glu Val Arg Glu Leu Leu Lys Ala Gly Ala Asp Val 
                165                 170                 175     
Asn Ala Lys Asn Ser Arg Gly Trp Thr Pro Leu His Thr Ala Ala Gln 
            180                 185                 190         
Thr Gly His Leu Glu Ile Phe Glu Val Leu Leu Lys Ala Gly Ala Asp 
        195                 200                 205             
Val Asn Ala Lys Asp Asp Lys Gly Val Thr Pro Leu His Leu Ala Ala 
    210                 215                 220                 
Ala Leu Gly His Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala 
225                 230                 235                 240 
Asp Val Asn Ala Gln Asp Ser Trp Gly Thr Thr Pro Ala Asp Leu Ala 
                245                 250                 255     
Ala Lys Tyr Gly His Glu Asp Ile Ala Glu Val Leu Gln Lys Ala Ala 
            260                 265                 270         



<210> 35
<211> 272
<212> PRT
<213> Artificial Sequence


<220> 
<223> Ankyrin repeat protein specific for HSA-CD3

<400> 35
Asp Leu Gly Lys Lys Leu Leu Glu Ala Ala Arg Ala Gly Gln Asp Asp 
1               5                   10                  15      
Glu Val Arg Glu Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp 
            20                  25                  30          
Tyr Phe Ser His Thr Pro Leu His Leu Ala Ala Arg Asn Gly His Leu 
        35                  40                  45              
Lys Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys 
    50                  55                  60                  
Asp Phe Ala Gly Lys Thr Pro Leu His Leu Ala Ala Ala Asp Gly His 
65                  70                  75                  80  
Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala 
                85                  90                  95      
Gln Asp Ile Phe Gly Lys Thr Pro Ala Asp Ile Ala Ala Asp Ala Gly 
            100                 105                 110         
His Glu Asp Ile Ala Glu Val Leu Gln Lys Ala Ala Gly Ser Pro Thr 
        115                 120                 125             
Pro Thr Pro Thr Thr Pro Thr Pro Thr Pro Thr Thr Pro Thr Pro Thr 
    130                 135                 140                 
Pro Thr Gly Ser Asp Leu Gly Gln Lys Leu Leu Glu Ala Ala Trp Ala 
145                 150                 155                 160 
Gly Gln Asp Asp Glu Val Arg Glu Leu Leu Lys Ala Gly Ala Asp Val 
                165                 170                 175     
Asn Ala Lys Asn Ser Arg Gly Trp Thr Pro Leu His Thr Ala Ala Gln 
            180                 185                 190         
Thr Gly His Leu Glu Ile Phe Glu Val Leu Leu Lys Ala Gly Ala Asp 
        195                 200                 205             
Val Asn Ala Lys Asn Asp Lys Arg Val Thr Pro Leu His Leu Ala Ala 
    210                 215                 220                 
Ala Leu Gly His Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala 
225                 230                 235                 240 
Asp Val Asn Ala Arg Asp Ser Trp Gly Thr Thr Pro Ala Asp Leu Ala 
                245                 250                 255     
Ala Lys Tyr Gly His Gln Asp Ile Ala Glu Val Leu Gln Lys Ala Ala 
            260                 265                 270         



<210> 36
<211> 272
<212> PRT
<213> Artificial Sequence


<220> 
<223> Ankyrin repeat protein specific for HSA-CD3

<400> 36
Asp Leu Gly Lys Lys Leu Leu Glu Ala Ala Arg Ala Gly Gln Asp Asp 
1               5                   10                  15      
Glu Val Arg Glu Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp 
            20                  25                  30          
Tyr Phe Ser His Thr Pro Leu His Leu Ala Ala Arg Asn Gly His Leu 
        35                  40                  45              
Lys Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys 
    50                  55                  60                  
Asp Phe Ala Gly Lys Thr Pro Leu His Leu Ala Ala Ala Asp Gly His 
65                  70                  75                  80  
Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala 
                85                  90                  95      
Gln Asp Ile Phe Gly Lys Thr Pro Ala Asp Ile Ala Ala Asp Ala Gly 
            100                 105                 110         
His Glu Asp Ile Ala Glu Val Leu Gln Lys Ala Ala Gly Ser Pro Thr 
        115                 120                 125             
Pro Thr Pro Thr Thr Pro Thr Pro Thr Pro Thr Thr Pro Thr Pro Thr 
    130                 135                 140                 
Pro Thr Gly Ser Asp Leu Gly Gln Lys Leu Leu Glu Ala Ala Trp Ala 
145                 150                 155                 160 
Gly Gln Leu Asp Glu Val Arg Ile Leu Leu Lys Ala Gly Ala Asp Val 
                165                 170                 175     
Asn Ala Lys Asn Ser Arg Gly Trp Thr Pro Leu His Thr Ala Ala Gln 
            180                 185                 190         
Thr Gly His Leu Glu Ile Phe Glu Val Leu Leu Lys Ala Gly Ala Asp 
        195                 200                 205             
Val Asn Ala Lys Thr Asn Lys Arg Val Thr Pro Leu His Leu Ala Ala 
    210                 215                 220                 
Ala Leu Gly His Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala 
225                 230                 235                 240 
Asp Val Asn Ala Arg Asp Thr Trp Gly Thr Thr Pro Ala Asp Leu Ala 
                245                 250                 255     
Ala Lys Tyr Gly His Arg Asp Ile Ala Glu Val Leu Gln Lys Ala Ala 
            260                 265                 270         



<210> 37
<211> 417
<212> DNA
<213> Artificial Sequence


<220> 
<223> Nucleic acid encoding domain of SEQ ID NO 1

<400> 37
atgagaggat cgcatcacca tcaccatcac ggctcagatc tgggtcaaaa gttattggag      60

gcagcttggg ctggacaaga tgacgaagta cgtgaattgt taaaagcggg agcagatgta     120

aacgcaaaag atagtcaagg ttggactccg ttacacacag cagcgcaaac cggccacctg     180

gaaattttcg aggtgttatt gaaggctgga gcagatgtga atgcaaaaga cgacaaaggg     240

gtgactccgc tgcatctggc agcggcgttg gggcacttgg aaatcgttga ggtccttctg     300

aaagcaggcg ctgatgtgaa tgcgcaagac tcctggggaa ccacaccagc ggacctggcg     360

gctaagtacg gccacgaaga tattgctgaa gttctgcaga aggcagcata atgatag        417


<210> 38
<211> 417
<212> DNA
<213> Artificial Sequence


<220> 
<223> Nucleic acid encoding domain of SEQ ID NO 2

<400> 38
atgagaggat cgcatcacca tcaccatcac ggctcagatc tgggtcaaaa gttgttggaa      60

gctgcctggg cgggacagga tgatgaggtg cgcgaattac ttaaggcggg agcagacgtg     120

aatgcgaaaa actctcgtgg ctggacacca ctgcacacgg ccgcgcaaac tggtcacctt     180

gaaattttcg aagtgcttct gaaggcaggc gcagatgtaa acgccaagga tgacaaaggg     240

gtaacaccgc ttcatctggc tgctgcactg ggacatcttg agattgtcga agtactgctt     300

aaggcaggtg ctgacgtaaa cgctcaggat tcatggggga ccacaccggc ggacctggcg     360

gctaaatacg gacatgaaga tattgctgaa gttctgcaga aggcagcata atgatag        417


<210> 39
<211> 417
<212> DNA
<213> Artificial Sequence


<220> 
<223> Nucleic acid encoding domain of SEQ ID NO 3

<400> 39
atgagaggat cgcatcacca tcaccatcac ggctcagatc tgggtcaaaa gctgttggaa      60

gccgcgtggg cgggtcagga cgatgaagtc cgtgagctgc ttaaagcagg agccgacgtg     120

aacgcgaaga actcacgcgg gtggacgcca cttcacacgg ccgcgcagac aggtcacctt     180

gaaatctttg aggttcttct gaaggcagga gcagacgtta acgccaaaaa cgacaagcgc     240

gtgactccgt tgcaccttgc cgcagctctg gggcatttgg agatcgttga ggtactgttg     300

aaagcgggag cagatgttaa tgctcgcgac agttggggga cgacaccagc agacctggcc     360

gcaaaatacg gacaccaaga cattgctgaa gttctgcaga aggcagcata atgatag        417


<210> 40
<211> 417
<212> DNA
<213> Artificial Sequence


<220> 
<223> Nucleic acid encoding domain of SEQ ID NO 4

<400> 40
atgagaggat cgcatcacca tcaccatcac ggctcagatc tgggtcaaaa attgttagag      60

gcagcctggg cgggacagtt agacgaggtt cgtattcttt tgaaagctgg tgcggatgtg     120

aacgcaaaga attctcgtgg atggactccg ttgcacaccg ccgcacagac tgggcacttg     180

gaaatctttg aggttctgtt aaaagcaggg gcagatgtta acgctaaaac taataaacgt     240

gtcacccccc ttcacctggc tgcggcttta ggccatttag aaatcgtgga agtattactt     300

aaagccgggg ctgacgttaa cgcccgtgac acttggggga caacccctgc ggatctggcc     360

gccaaatatg gtcaccgcga cattgctgaa gttctgcaga aggcagcata atgatag        417


<210> 41
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> Consensus GS linker

<220> 
<221> VARIANT
<222> 1..5
<223> [Gly-Gly-Gly-Gly-Ser]n, wherein n is 1, 2, 3, 4, 5, or 6

<400> 41
Gly Gly Gly Gly Ser 
1               5   

<210> 42
<211> 24
<212> PRT
<213> Artificial Sequence


<220> 
<223> PT-rich peptide linker (standard)

<400> 42
Gly Ser Pro Thr Pro Thr Pro Thr Thr Pro Thr Pro Thr Pro Thr Thr 
1               5                   10                  15      
Pro Thr Pro Thr Pro Thr Gly Ser 
            20                  

<210> 43
<211> 38
<212> PRT
<213> Artificial Sequence


<220> 
<223> Linker L

<400> 43
Gly Ser Pro Thr Pro Thr Pro Thr Thr Pro Thr Pro Thr Pro Thr Thr 
1               5                   10                  15      
Pro Thr Pro Thr Pro Thr Thr Pro Thr Pro Thr Pro Thr Thr Pro Thr 
            20                  25                  30          
Pro Thr Pro Thr Gly Ser 
        35              

<210> 44
<211> 18
<212> PRT
<213> Artificial Sequence


<220> 
<223> Linker S

<400> 44
Gly Ser Pro Thr Pro Thr Pro Thr Thr Pro Thr Pro Thr Pro Thr Thr 
1               5                   10                  15      
Gly Ser 
        

<210> 45
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> Linker XS

<400> 45
Gly Ser Pro Thr Pro Thr Pro Thr Thr Gly Ser 
1               5                   10      

<210> 46
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> Linker XXS

<400> 46
Gly Ser Pro Thr Gly Ser 
1               5       

