                         序列表

<110>  永农生物科学有限公司
 
<120>  利用生物多酶偶联法制备L-草铵膦的方法

<130>  PD200280N

<150>  CN202011496575.9
<151>  2020－12－17

<160>  4     

<170>  PatentIn版本 3.5

<210>  1
<211>  309
<212>  PRT
<213>  Pseudarthrobacter chlorophenolicus

<400>  1

Met Thr Ser Pro Ala Ser Val Val Leu Val Phe Leu Asp Pro Ala Phe 
1               5                   10                  15      


Pro Asp Gly Arg Leu Ala Asp Ala Ala Gln Pro Gln Leu Met Val Thr 
            20                  25                  30          


Asp Gln Gly Ala Thr Arg Gly Asp Gly Ile Phe Glu Thr Met Leu Ala 
        35                  40                  45              


Val Arg Gly Ser Val Arg Lys Ile Gln Ala His Leu Asp Arg Leu Asp 
    50                  55                  60                  


Gly Ser Ala Ala Ala Leu Asp Leu Ser Ile Pro Gly Gln Asp Asp Trp 
65                  70                  75                  80  


Arg Arg Ala Ile Ala Thr Ala Ile Ala Glu His Gln Ala Gln Tyr Pro 
                85                  90                  95      


Ala Pro Asp Ala Gly Asp Asp Glu Leu Val Val Lys Leu Val Val Thr 
            100                 105                 110         


Arg Gly Val Glu Gly Ala Gly Ser Pro Thr Ala Trp Val Gln Val Ser 
        115                 120                 125             


Pro Ala Pro Ala Ala Gly Arg Arg Gln Arg Glu Thr Gly Ile Asp Val 
    130                 135                 140                 


Ile Leu Leu Asp Arg Gly Tyr Asp Ser Asp Val Ala Glu Arg Ala Pro 
145                 150                 155                 160 


Trp Leu Leu Met Gly Ala Lys Thr Leu Ser Tyr Ala Val Asn Met Ala 
                165                 170                 175     


Ala Leu Arg His Ala Arg Arg Gln Gly Ala Asp Asp Val Ile Phe Leu 
            180                 185                 190         


Ser Ser Asp Gly Arg Val Leu Glu Gly Pro Thr Ser Thr Val Leu Leu 
        195                 200                 205             


Ala His Val Glu Glu Ser Ala Asp Gly Thr Ala Ile Lys Arg Leu Ile 
    210                 215                 220                 


Thr Pro Gln Leu Asp Ser Gly Ile Leu Pro Gly Thr Ser Gln Gly Ala 
225                 230                 235                 240 


Leu Phe Thr Ala Ala Lys Ala Ala Gly Trp Glu Leu Gly Tyr Gly Pro 
                245                 250                 255     


Leu Glu Pro Gln Asp Leu Leu Asp Ala Asp Ala Val Trp Leu Ile Ser 
            260                 265                 270         


Ser Val Arg Leu Leu Ala Pro Val Asn Thr Ile Asp Gly Lys Gln Ile 
        275                 280                 285             


Gly Thr Pro Ala Leu Gln Lys Glu Leu Thr Ala Glu Leu Thr Gly Leu 
    290                 295                 300                 


Tyr Ala Gly Ile Gln 
305                 


<210>  2
<211>  930
<212>  DNA
<213>  Pseudarthrobacter chlorophenolicus

<400>  2
atgacctctc ccgcttccgt ggtactcgtt ttccttgatc ccgccttccc cgacggccgg       60

ctggccgacg ccgcccagcc gcagctgatg gtcacggacc agggcgccac caggggcgat      120

ggcatcttcg aaacgatgct cgccgtgcgc gggtcagtcc gaaaaatcca ggcccacctg      180

gaccgcctgg acggctccgc ggcggcgctg gacctcagca tcccgggcca ggatgactgg      240

cggcgggcca ttgccactgc cattgccgaa caccaggcgc agtacccggc ccccgatgcg      300

ggcgacgatg aactggtggt caagctggtg gtcacccgcg gcgttgaagg tgcgggctcc      360

cccaccgcct gggtgcaggt ctcccctgct ccggccgccg gccgccgcca acgggaaaca      420

ggcatcgacg tcatcctcct tgaccgcggg tacgacagtg acgttgccga gcgtgcgccg      480

tggctgctca tgggcgccaa gacgctctcc tacgccgtca acatggccgc cctgcgccat      540

gcccgcaggc agggcgcaga cgacgtcatc ttcctgtcct cggatggccg cgtgcttgag      600

ggccccacgt ccacggtgct gctggcgcac gtggaggagt ccgctgacgg gacggccatc      660

aagcgcctca tcacgccgca gctggacagc ggcatcctgc ccggaacatc gcagggggcc      720

ctcttcaccg cggcaaaggc ggcgggctgg gaactgggct acggacccct ggaaccgcag      780

gacctgctgg atgccgacgc ggtctggctg atctccagtg tccgcctcct cgccccggtc      840

aacacgatcg acggcaagca gatcggtacc ccggcgctgc agaaggagct gacggctgag      900

ctcacggggc tttacgccgg aatccaatag                                       930


<210>  3
<211>  353
<212>  PRT
<213>  Corynebacterium vitaeruminis DSM 20294

<400>  3

Met Thr Pro Gln Gly Thr Phe Phe Leu Pro Gly Pro Thr Glu Val His 
1               5                   10                  15      


Pro Asp Val Leu Ala Ala Gln Thr Arg Pro Met Ile Gly His Arg Gly 
            20                  25                  30          


Ala Ala Ile Gln Thr Leu Met Ala Asn Ile Ala Thr Gly Met Arg Ser 
        35                  40                  45              


Val Phe Gln Thr Glu Arg Pro Val Ile Val Ser Thr Ser Ser Ala Ser 
    50                  55                  60                  


Gly Leu Met Glu Gly Ala Ile Arg Asn Gly Val Gly Thr Gly Pro Val 
65                  70                  75                  80  


Leu Cys Leu Val Asn Gly Ala Phe Ser Gly Arg Phe Ala Thr Ile Ala 
                85                  90                  95      


Lys Ala Cys Gly Lys Asp Thr Glu Ile Trp Glu Val Glu Trp Gly Thr 
            100                 105                 110         


Thr His Ser Val Asp Glu Leu Ala Arg Arg Leu Ser Glu Arg Arg Tyr 
        115                 120                 125             


Asp Ala Val Thr Leu Ser Gln Ser Glu Thr Ser Thr Gly Ala Leu His 
    130                 135                 140                 


Asp Leu Glu Arg Val Ala Ala Val Val Ala Glu His Pro Gly Thr Leu 
145                 150                 155                 160 


Leu Leu Val Asp Ser Val Thr Gly Ile Gly Gly Val Glu Thr Arg Pro 
                165                 170                 175     


Asp Asp Trp Gly Ile Asp Phe Ile Leu Thr Gly Ser Gln Lys Ala Leu 
            180                 185                 190         


Ala Leu Pro Pro Gly Leu Ala Phe Gly Ala Ala Ser Glu Ala Met Met 
        195                 200                 205             


Glu Arg Ala Ala Ser Ile Pro Asp Arg Gly Trp Tyr Phe Asp Leu Val 
    210                 215                 220                 


Lys Leu Ala Glu Gly Ile Glu Lys Asn Gln Thr Pro Ala Thr Pro Ala 
225                 230                 235                 240 


Ile Ser Leu Leu Tyr Ser Leu Glu Tyr Gln Leu Glu Arg Ile Leu Ala 
                245                 250                 255     


Glu Gly Ile Glu Ala Arg Trp Lys Arg His Leu Gln Met Gln Ala Arg 
            260                 265                 270         


Val Leu Glu Trp Val Glu Asp Ala Gly Leu Asp Val Leu Ala Val Glu 
        275                 280                 285             


Gly His Arg Ser Pro Thr Val Thr Cys Val Val His Pro Glu Pro Gly 
    290                 295                 300                 


Arg Val Val Gly Ala Met Ala Glu Arg Gly Trp Val Ile Gly Ala Gly 
305                 310                 315                 320 


Tyr Gly Gln Val Ser Asp Arg Thr Phe Arg Ile Gly His Met Gly Asp 
                325                 330                 335     


His Thr Leu Glu Glu Val Asp Ala Leu Leu Ala Val Ile Gly Glu Val 
            340                 345                 350         


Ile 
    


<210>  4
<211>  1062
<212>  DNA
<213>  Corynebacterium vitaeruminis DSM 20294

<400>  4
atgacgccac agggcacgtt cttcctcccg gggcctaccg aggtccaccc cgacgtactc       60

gccgcccaga ctcgaccgat gatcggtcat cgcggtgcgg ccatccagac cctgatggcg      120

aacatcgcca ccggcatgag gtctgtattc cagaccgagc ggcccgtgat cgtctcgacc      180

tcgtcggcgt cggggctcat ggaaggggcc atccgcaacg gcgtgggaac gggcccggtg      240

ctgtgtctcg tcaacggcgc gttctcgggt cgcttcgcca cgatcgccaa ggcctgtggc      300

aaggacaccg agatctggga ggtggagtgg ggaacgacgc actccgtcga cgaacttgcc      360

cgtcggctgt ccgagcgacg ctacgacgcg gtgaccctct cccagtccga gacgtccacc      420

ggtgcgctcc acgacctgga gagggtcgct gccgtggtcg cagagcaccc cggcacactg      480

ctactcgtcg actccgtcac cggaatcggc ggtgtggaga cgcggccgga cgactggggc      540

atcgacttca tcctgaccgg ctcccagaag gccctggcac tgccgcccgg gctcgcattc      600

ggcgccgcct cggaggccat gatggagcgg gccgcctcga tccccgaccg tggctggtac      660

ttcgacctgg tcaagctcgc cgagggcatc gagaagaacc agaccccggc gactcctgcg      720

atctcgctcc tctattcgct ggagtaccag ctggagagga tcctcgccga gggcatcgag      780

gcgcgctgga agcggcacct gcagatgcag gcccgggtgc tcgagtgggt cgaggatgcc      840

ggcctcgatg ttctcgctgt cgaaggccat cggtccccga ccgtgacctg cgtggtccac      900

ccggagccgg gacgcgtggt cggggcgatg gccgagcgcg ggtgggtgat cggggcgggc      960

tacggccagg tgagcgaccg gacgttccgg atcgggcata tgggcgacca cacgctcgaa     1020

gaggtcgacg ccctcctcgc cgtgataggg gaagtcatct ga                        1062


