﻿               SEQUENCE LISTING

<110> 榕森生物科技（北京）有限公司
      中国科学院生物物理研究所

<120> 病原样抗原疫苗及其制备方法

<130> CIE210131PCT

<160> 26

<170> BiSSAP 1.3.6

<210> 1
<211> 136
<212> PRT
<213> Artificial Sequence


<220> 
<223> AP205氨基酸序列

<400> 1
Met Glu Phe Gly Ser Met Ala Asn Lys Pro Met Gln Pro Ile Thr Ser 
1               5                   10                  15      
Thr Ala Asn Lys Ile Val Trp Ser Asp Pro Thr Arg Leu Ser Thr Thr 
            20                  25                  30          
Phe Ser Ala Ser Leu Leu Arg Gln Arg Val Lys Val Gly Ile Ala Glu 
        35                  40                  45              
Leu Asn Asn Val Ser Gly Gln Tyr Val Ser Val Tyr Lys Arg Pro Ala 
    50                  55                  60                  
Pro Lys Pro Glu Gly Cys Ala Asp Ala Cys Val Ile Met Pro Asn Glu 
65                  70                  75                  80  
Asn Gln Ser Ile Arg Thr Val Ile Ser Gly Ser Ala Glu Asn Leu Ala 
                85                  90                  95      
Thr Leu Lys Ala Glu Trp Glu Thr His Lys Arg Asn Val Asp Thr Leu 
            100                 105                 110         
Phe Ala Ser Gly Asn Ala Gly Leu Gly Phe Leu Asp Pro Thr Ala Ala 
        115                 120                 125             
Ile Val Ser Ser Asp Thr Thr Ala 
    130                 135     

<210> 2
<211> 223
<212> PRT
<213> Artificial Sequence


<220> 
<223> 新冠RBD氨基酸序列

<400> 2
Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn 
1               5                   10                  15      
Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
            20                  25                  30          
Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
        35                  40                  45              
Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
    50                  55                  60                  
Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
65                  70                  75                  80  
Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
                85                  90                  95      
Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
            100                 105                 110         
Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
        115                 120                 125             
Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
    130                 135                 140                 
Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
145                 150                 155                 160 
Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
                165                 170                 175     
Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190         
Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
        195                 200                 205             
Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Ser Val Asn Phe 
    210                 215                 220             

<210> 3
<211> 15
<212> PRT
<213> Artificial Sequence


<220> 
<223> ST氨基酸序列

<400> 3
Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys Thr Gly 
1               5                   10                  15  

<210> 4
<211> 92
<212> PRT
<213> Artificial Sequence


<220> 
<223> SC氨基酸序列

<400> 4
Asp Ser Ala Thr His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Lys 
1               5                   10                  15      
Glu Leu Ala Gly Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr 
            20                  25                  30          
Ile Ser Thr Trp Ile Ser Asp Gly Gln Val Lys Asp Phe Tyr Leu Tyr 
        35                  40                  45              
Pro Gly Lys Tyr Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu 
    50                  55                  60                  
Val Ala Thr Ala Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val Thr 
65                  70                  75                  80  
Val Asn Gly Lys Ala Thr Lys Gly Asp Ala His Ile 
                85                  90          

<210> 5
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> 第一连接肽氨基酸序列

<400> 5
Gly Ser Gly Gly Ser Gly Thr Gly Gly Ser Gly 
1               5                   10      

<210> 6
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> 第二连接肽氨基酸序列

<400> 6
Gly Ser Gly Gly Ser Gly 
1               5       

<210> 7
<211> 162
<212> PRT
<213> Artificial Sequence


<220> 
<223> AP205-第一连接肽-ST氨基酸序列

<400> 7
Met Glu Phe Gly Ser Met Ala Asn Lys Pro Met Gln Pro Ile Thr Ser 
1               5                   10                  15      
Thr Ala Asn Lys Ile Val Trp Ser Asp Pro Thr Arg Leu Ser Thr Thr 
            20                  25                  30          
Phe Ser Ala Ser Leu Leu Arg Gln Arg Val Lys Val Gly Ile Ala Glu 
        35                  40                  45              
Leu Asn Asn Val Ser Gly Gln Tyr Val Ser Val Tyr Lys Arg Pro Ala 
    50                  55                  60                  
Pro Lys Pro Glu Gly Cys Ala Asp Ala Cys Val Ile Met Pro Asn Glu 
65                  70                  75                  80  
Asn Gln Ser Ile Arg Thr Val Ile Ser Gly Ser Ala Glu Asn Leu Ala 
                85                  90                  95      
Thr Leu Lys Ala Glu Trp Glu Thr His Lys Arg Asn Val Asp Thr Leu 
            100                 105                 110         
Phe Ala Ser Gly Asn Ala Gly Leu Gly Phe Leu Asp Pro Thr Ala Ala 
        115                 120                 125             
Ile Val Ser Ser Asp Thr Thr Ala Gly Ser Gly Gly Ser Gly Thr Gly 
    130                 135                 140                 
Gly Ser Gly Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys 
145                 150                 155                 160 
Thr Gly 
        

<210> 8
<211> 321
<212> PRT
<213> Artificial Sequence


<220> 
<223> SC-第二连接肽-RBD氨基酸序列

<400> 8
Asp Ser Ala Thr His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Lys 
1               5                   10                  15      
Glu Leu Ala Gly Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr 
            20                  25                  30          
Ile Ser Thr Trp Ile Ser Asp Gly Gln Val Lys Asp Phe Tyr Leu Tyr 
        35                  40                  45              
Pro Gly Lys Tyr Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu 
    50                  55                  60                  
Val Ala Thr Ala Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val Thr 
65                  70                  75                  80  
Val Asn Gly Lys Ala Thr Lys Gly Asp Ala His Ile Gly Ser Gly Gly 
                85                  90                  95      
Ser Gly Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile 
            100                 105                 110         
Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala 
        115                 120                 125             
Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp 
    130                 135                 140                 
Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr 
145                 150                 155                 160 
Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr 
                165                 170                 175     
Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro 
            180                 185                 190         
Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp 
        195                 200                 205             
Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys 
    210                 215                 220                 
Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn 
225                 230                 235                 240 
Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly 
                245                 250                 255     
Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu 
            260                 265                 270         
Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr 
        275                 280                 285             
Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val 
    290                 295                 300                 
Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Ser Val Asn 
305                 310                 315                 320 
Phe 
    

<210> 9
<211> 393
<212> DNA
<213> Artificial Sequence


<220> 
<223> AP205 cDNA序列

<400> 9
atggcaaata agccaatgca accgatcaca tctacagcaa ataaaattgt gtggagtgat      60

ccaactcgtt tatcaactac attttcagca agtctgttac gccaacgtgt taaagttggt     120

atagccgaac tgaataatgt ttcaggtcaa tatgtatctg tttataagcg tcctgcacct     180

aaaccggaag gttgtgcaga tgcctgtgtc attatgccga atgaaaacca atccattcgc     240

acagtgattt cagggtcagc cgaaaacttg gctaccttaa aagcagaatg ggaaactcac     300

aaacgtaacg ttgacacact cttcgcgagc ggcaacgccg gtttgggttt ccttgaccct     360

actgcggcta tcgtatcgtc tgatactact gct                                  393


<210> 10
<211> 276
<212> DNA
<213> Artificial Sequence


<220> 
<223> SC cDNA序列

<400> 10
gatagcgcta cccatatcaa attctcaaaa cgtgatgagg acggcaaaga gttagctggt     60

gcaactatgg agttgcgtga ttcatctggt aaaactatta gtacatggat ttcagatgga    120

caagtgaaag atttctacct gtatccagga aaatatacat ttgtcgaaac cgcagcacca    180

gacggttatg aggtagcaac tgctattacc tttacagtta atgagcaagg tcaggttact    240

gtaaatggca aagcaactaa aggtgacgct catatt                              276


<210> 11
<211> 1068
<212> DNA
<213> Artificial Sequence


<220> 
<223> RBD-SC cDNA序列

<400> 11
atgttcgtgt ttctggtgct gctgcctctg gtgagctccc agtgcaccgg tagggtgcag      60

cctaccgagt ctatcgtgcg cttcccaaac atcacaaatc tgtgcccctt cggcgaggtg     120

tttaatgcca cccggtttgc cagcgtgtat gcctggaatc ggaagagaat ctccaactgc     180

gtggccgact attctgtgct gtacaacagc gccagcttca gcaccttcaa gtgctatggc     240

gtgtccccca ccaagctgaa tgacctgtgc ttcacaaacg tgtacgccga ctcttttgtg     300

atccggggcg atgaggtgag acagatcgca ccaggacaga ccggcaagat cgcagactac     360

aattataagc tgcctgacga tttcacaggc tgcgtgatcg cctggaacag caacaatctg     420

gattccaaag tgggcggcaa ctacaattat ctgtacaggc tgttccgcaa gtccaatctg     480

aagccatttg agagggacat cagcaccgag atctaccagg ccggctccac accctgcaat     540

ggcgtggagg gcttcaactg ttattttcct ctgcagagct acggcttcca gccaaccaac     600

ggcgtgggct atcagcccta ccgcgtggtg gtgctgtcct ttgagctgct gcacgcacca     660

gcaaccgtgt gcggacctaa gaagtctaca aacctggtga agaataagag cgtgaacttt     720

ggaggaggag gaggaggaag caagcttgac tctgccaccc acatcaagtt cagcaagagg     780

gacgaggatg gcaaggagct ggcaggagca acaatggagc tgagagatag ctccggcaag     840

accatctcca catggatctc tgacggccag gtgaaggatt tctacctgta tcctggcaag     900

tatacctttg tggagacagc agcaccagac ggatacgagg tggcaaccgc catcaccttt     960

acagtgaatg agcagggcca ggtgaccgtg aacggcaagg ccacaaaggg cgatgcacac    1020

atcggaggaa gccatcatca tcatcatcac catcatcatc atcatcac                 1068


<210> 12
<211> 1059
<212> DNA
<213> Artificial Sequence


<220> 
<223> SC-RBD cDNA序列

<400> 12
atgttcgtgt ttctggtgct gctgcctctg gtgagctccc agtgcgactc tgccacccac      60

atcaagttca gcaagaggga cgaggatggc aaggagctgg caggagcaac aatggagctg     120

agagatagct ccggcaagac catctccaca tggatctctg acggccaggt gaaggatttc     180

tacctgtatc ctggcaagta tacctttgtg gagacagcag caccagacgg atacgaggtg     240

gcaaccgcca tcacctttac agtgaatgag cagggccagg tgaccgtgaa cggcaaggcc     300

acaaagggcg atgcacacat cggatctgga ggaagcggaa gggtgcagcc taccgagtct     360

atcgtgcgct tcccaaacat cacaaatctg tgccccttcg gcgaggtgtt taatgccacc     420

cggtttgcca gcgtgtatgc ctggaatcgg aagagaatct ccaactgcgt ggccgactat     480

tctgtgctgt acaacagcgc cagcttcagc accttcaagt gctatggcgt gtcccccacc     540

aagctgaatg acctgtgctt cacaaacgtg tacgccgact cttttgtgat ccggggcgat     600

gaggtgagac agatcgcacc aggacagacc ggcaagatcg cagactacaa ttataagctg     660

cctgacgatt tcacaggctg cgtgatcgcc tggaacagca acaatctgga ttccaaagtg     720

ggcggcaact acaattatct gtacaggctg ttccgcaagt ccaatctgaa gccatttgag     780

agggacatca gcaccgagat ctaccaggcc ggctccacac cctgcaatgg cgtggagggc     840

ttcaactgtt attttcctct gcagagctac ggcttccagc caaccaacgg cgtgggctat     900

cagccctacc gcgtggtggt gctgtccttt gagctgctgc acgcaccagc aaccgtgtgc     960

ggacctaaga agtctacaaa cctggtgaag aataagagcg tgaactttgg aggaggagga    1020

agccatcatc atcatcatca ccatcatcat catcatcac                           1059


<210> 13
<211> 31
<212> DNA
<213> Artificial Sequence


<220> 
<223> SC-AP205表达质粒上游引物序列

<400> 13
acgggatcca tgtcgtacta ccatcaccat c                                   31


<210> 14
<211> 48
<212> DNA
<213> Artificial Sequence


<220> 
<223> SC-AP205表达质粒下游引物序列

<400> 14
cccggatcca ctgccgctac ctccaatatg agcgtcacct ttagttgc                  48


<210> 15
<211> 31
<212> DNA
<213> Artificial Sequence


<220> 
<223> AP205-SC表达质粒上游引物序列

<400> 15
acgaccggta tgtcgtacta ccatcaccat c                                   31


<210> 16
<211> 33
<212> DNA
<213> Artificial Sequence


<220> 
<223> AP205-SC表达质粒下游引物序列

<400> 16
cccaccggta atatgagcgt cacctttagt tgc                                  33


<210> 17
<211> 54
<212> DNA
<213> Artificial Sequence


<220> 
<223> AP205-ST表达质粒上游引物序列

<400> 17
ccggtggtag cggcgcccac atcgtgatgg tggacgccta caagccgacg aaga           54


<210> 18
<211> 54
<212> DNA
<213> Artificial Sequence


<220> 
<223> AP205-ST表达质粒下游引物序列

<400> 18
ccggtcttcg tcggcttgta ggcgtccacc atcacgatgt gggcgccgct acca           54


<210> 19
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> 实施例7中的上游引物序列

<400> 19
caatggttta acaggcacag g                                              21


<210> 20
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> 实施例7中的下游引物序列

<400> 20
ctcaagtgtc tgtggatcac g                                              21


<210> 21
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> 实施例10中的上游引物序列

<400> 21
ggggaacttc tcctgctaga at                                             22


<210> 22
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> 实施例10中的下游引物

<400> 22
cagacatttt gctctcaagc tg                                             22


<210> 23
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> 实施例10中的探针序列

<400> 23
ttgctgctgc ttgacagatt                                                20


<210> 24
<211> 148
<212> PRT
<213> Artificial Sequence


<220> 
<223> 非洲猪瘟病毒抗原eP22氨基酸序列

<400> 24
Lys Lys Gln Gln Pro Pro Lys Lys Val Cys Lys Val Asp Lys Asp Cys 
1               5                   10                  15      
Gly Ser Gly Glu His Cys Val Arg Gly Ser Cys Ser Ser Leu Ser Cys 
            20                  25                  30          
Leu Asp Ala Val Lys Met Asp Lys Arg Asn Ile Lys Ile Asp Ser Lys 
        35                  40                  45              
Ile Ser Ser Cys Glu Phe Thr Pro Asn Phe Tyr Arg Phe Thr Asp Thr 
    50                  55                  60                  
Ala Ala Asp Glu Gln Gln Glu Phe Gly Lys Thr Arg His Pro Ile Lys 
65                  70                  75                  80  
Ile Thr Pro Ser Pro Ser Glu Ser His Ser Pro Gln Glu Val Cys Glu 
                85                  90                  95      
Lys Tyr Cys Ser Trp Gly Thr Asp Asp Cys Thr Gly Trp Glu Tyr Val 
            100                 105                 110         
Gly Asp Glu Lys Glu Gly Thr Cys Tyr Val Tyr Asn Asn Pro His His 
        115                 120                 125             
Pro Val Leu Lys Tyr Gly Lys Asp His Ile Ile Ala Leu Pro Arg Asn 
    130                 135                 140                 
His Lys His Ala 
145             

<210> 25
<211> 23
<212> PRT
<213> Artificial Sequence


<220> 
<223> 流感病毒抗原M2E氨基酸序列

<400> 25
Ser Leu Leu Thr Glu Val Glu Thr Pro Ile Arg Asn Glu Trp Gly Cys 
1               5                   10                  15      
Arg Cys Asn Gly Ser Ser Asp 
            20              

<210> 26
<211> 117
<212> PRT
<213> Artificial Sequence


<220> 
<223> 自身抗原髓鞘少突胶质细胞糖蛋白氨基酸序列

<400> 26
Gly Gln Phe Arg Val Ile Gly Pro Gly Tyr Pro Ile Arg Ala Leu Val 
1               5                   10                  15      
Gly Asp Glu Ala Glu Leu Pro Cys Arg Ile Ser Pro Gly Lys Asn Ala 
            20                  25                  30          
Thr Gly Met Glu Val Gly Trp Tyr Arg Ser Pro Phe Ser Arg Val Val 
        35                  40                  45              
His Leu Tyr Arg Asn Gly Lys Asp Gln Asp Ala Glu Gln Ala Pro Glu 
    50                  55                  60                  
Tyr Arg Gly Arg Thr Glu Leu Leu Lys Glu Thr Ile Ser Glu Gly Lys 
65                  70                  75                  80  
Val Thr Leu Arg Ile Gln Asn Val Arg Phe Ser Asp Glu Gly Gly Tyr 
                85                  90                  95      
Thr Cys Phe Phe Arg Asp His Ser Tyr Gln Glu Glu Ala Ala Met Glu 
            100                 105                 110         
Leu Lys Val Glu Asp 
        115         

