                         SEQUENCE LISTING

<110>  Regents of the University of Minnesota
       Moriarity, Branden
       Webber, Beau
       Yamamoto, Kenta
 
<120>  DELIVERY OF MOLECULES TO CELLS USING TROGOCYTOSIS AND ENGINEERED 
       CELLS

<130>  920171.00446

<150>  63/122,590
<151>  2020-12-08

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  378
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Asp Leu Gly Lys Pro Met Lys Ser Val Leu Val Val Ala Leu Leu 
1               5                   10                  15      


Val Ile Phe Gln Val Cys Leu Cys Gln Asp Glu Val Thr Asp Asp Tyr 
            20                  25                  30          


Ile Gly Asp Asn Thr Thr Val Asp Tyr Thr Leu Phe Glu Ser Leu Cys 
        35                  40                  45              


Ser Lys Lys Asp Val Arg Asn Phe Lys Ala Trp Phe Leu Pro Ile Met 
    50                  55                  60                  


Tyr Ser Ile Ile Cys Phe Val Gly Leu Leu Gly Asn Gly Leu Val Val 
65                  70                  75                  80  


Leu Thr Tyr Ile Tyr Phe Lys Arg Leu Lys Thr Met Thr Asp Thr Tyr 
                85                  90                  95      


Leu Leu Asn Leu Ala Val Ala Asp Ile Leu Phe Leu Leu Thr Leu Pro 
            100                 105                 110         


Phe Trp Ala Tyr Ser Ala Ala Lys Ser Trp Val Phe Gly Val His Phe 
        115                 120                 125             


Cys Lys Leu Ile Phe Ala Ile Tyr Lys Met Ser Phe Phe Ser Gly Met 
    130                 135                 140                 


Leu Leu Leu Leu Cys Ile Ser Ile Asp Arg Tyr Val Ala Ile Val Gln 
145                 150                 155                 160 


Ala Val Ser Ala His Arg His Arg Ala Arg Val Leu Leu Ile Ser Lys 
                165                 170                 175     


Leu Ser Cys Val Gly Ile Trp Ile Leu Ala Thr Val Leu Ser Ile Pro 
            180                 185                 190         


Glu Leu Leu Tyr Ser Asp Leu Gln Arg Ser Ser Ser Glu Gln Ala Met 
        195                 200                 205             


Arg Cys Ser Leu Ile Thr Glu His Val Glu Ala Phe Ile Thr Ile Gln 
    210                 215                 220                 


Val Ala Gln Met Val Ile Gly Phe Leu Val Pro Leu Leu Ala Met Ser 
225                 230                 235                 240 


Phe Cys Tyr Leu Val Ile Ile Arg Thr Leu Leu Gln Ala Arg Asn Phe 
                245                 250                 255     


Glu Arg Asn Lys Ala Ile Lys Val Ile Ile Ala Val Val Val Val Phe 
            260                 265                 270         


Ile Val Phe Gln Leu Pro Tyr Asn Gly Val Val Leu Ala Gln Thr Val 
        275                 280                 285             


Ala Asn Phe Asn Ile Thr Ser Ser Thr Cys Glu Leu Ser Lys Gln Leu 
    290                 295                 300                 


Asn Ile Ala Tyr Asp Val Thr Tyr Ser Leu Ala Cys Val Arg Cys Cys 
305                 310                 315                 320 


Val Asn Pro Phe Leu Tyr Ala Phe Ile Gly Val Lys Phe Arg Asn Asp 
                325                 330                 335     


Leu Phe Lys Leu Phe Lys Asp Leu Gly Cys Leu Ser Gln Glu Gln Leu 
            340                 345                 350         


Arg Gln Trp Ser Ser Cys Arg His Ile Arg Arg Ser Ser Met Ser Val 
        355                 360                 365             


Glu Ala Glu Thr Thr Thr Thr Phe Ser Pro 
    370                 375             


<210>  2
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- XTEN linker

<400>  2

Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser 
1               5                   10                  15  


<210>  3
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- FurinSite

<400>  3

Arg Arg Lys Arg 
1               


<210>  4
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- GFP

<400>  4

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 


<210>  5
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- NLS

<400>  5

Pro Lys Lys Lys Arg Lys Val 
1               5           


<210>  6
<211>  73
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- TfnR (TM)

<400>  6

Met Met Val Asp Gly Asp Asn Ser His Val Glu Met Lys Leu Ala Val 
1               5                   10                  15      


Asp Glu Glu Glu Asn Ala Asp Asn Asn Thr Lys Ala Asn Val Thr Lys 
            20                  25                  30          


Pro Lys Arg Cys Ser Gly Ser Ile Cys Tyr Gly Thr Ile Ala Val Ile 
        35                  40                  45              


Val Phe Phe Leu Ile Gly Phe Met Ile Gly Tyr Leu Gly Tyr Cys Lys 
    50                  55                  60                  


Ser Ser Asp Gly Pro Gly Glu Thr Gly 
65                  70              


<210>  7
<211>  1962
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- CCR7-XTEN-FurinSite-GFP

<400>  7
atggacctgg gcaagcccat gaagtccgtg ctggtggtgg ccctgctggt catctttcag       60

gtgtgcctgt gccaggatga ggtgacagac gattacatcg gcgacaatac cacagtggat      120

tataccctgt tcgagtctct gtgcagcaag aaggacgtgc ggaacttcaa ggcctggttt      180

ctgcctatca tgtacagcat catctgtttc gtgggcctgc tgggcaatgg cctggtggtg      240

ctgacataca tctacttcaa gcggctgaag accatgacag acacctacct gctgaacctg      300

gccgtggccg atatcctgtt cctgctgacc ctgccatttt gggcctattc tgccgccaag      360

agctgggtgt tcggcgtgca cttttgcaag ctgatctttg ccatctacaa gatgtccttc      420

ttttctggca tgctgctgct gctgtgcatc agcatcgaca gatatgtggc aatcgtgcag      480

gccgtgtccg cccaccggca cagagccagg gtgctgctga tcagcaagct gtcctgcgtg      540

ggcatctgga tcctggccac cgtgctgtcc atccctgagc tgctgtactc tgatctgcag      600

agaagctcct ctgagcaggc catgaggtgt tccctgatca cagagcacgt ggaggccttc      660

atcaccatcc aggtggccca gatggtcatc ggcttcctgg tgccactgct ggccatgagc      720

ttttgctacc tggtcatcat cagaaccctg ctgcaggccc gcaactttga gcggaataag      780

gccatcaaag tgatcatcgc cgtggtggtg gtgttcatcg tgtttcagct gccctataat      840

ggcgtggtgc tggcccagac agtggccaac ttcaatatca caagctccac ctgcgagctg      900

tctaagcagc tgaacatcgc ctacgacgtg acctatagcc tggcatgcgt gcggtgctgc      960

gtgaacccct tcctgtatgc ctttatcggc gtgaagtttc gcaatgacct gttcaagctg     1020

tttaaggatc tgggatgcct gagccaggag cagctgaggc agtggtctag ctgtcggcac     1080

atccggagat cctctatgtc cgtggaggcc gagaccacaa ccacattctc tcctagcgga     1140

ggaagctccg gaggatctag cggatctgag acaccaggca cctccgagtc tgccacacca     1200

gagtcctctg gcggcagctc cggaggaagc aggcgcaaga gaatggtgtc caagggagag     1260

gagctgttca ccggagtggt gcctatcctg gtggagctgg acggcgatgt gaatggccac     1320

aagtttagcg tgtccggaga gggagagggc gatgcaacat acggcaagct gaccctgaag     1380

ttcatctgca ccacaggcaa gctgcccgtg ccttggccaa cactggtgac cacactgacc     1440

tacggcgtgc agtgtttctc caggtatcct gaccacatga agcagcacga tttctttaag     1500

tctgccatgc cagagggcta cgtgcaggag cgcaccatct tctttaagga cgatggcaac     1560

tataagacac gggccgaggt gaagttcgag ggcgacaccc tggtgaacag aatcgagctg     1620

aagggcatcg actttaagga ggatggcaat atcctgggcc acaagctgga gtacaactat     1680

aattcccaca acgtgtacat catggccgat aagcagaaga acggcatcaa ggtgaacttc     1740

aagatcaggc acaatatcga ggacggcagc gtgcagctgg ccgatcacta ccagcagaac     1800

acaccaatcg gcgacggacc cgtgctgctg cctgataatc actatctgtc tacccagagc     1860

gccctgtcca aggaccccaa cgagaagcgc gatcacatgg tgctgctgga gttcgtgaca     1920

gcagcaggaa tcaccctggg aatggacgag ctgtataagt ga                        1962


<210>  8
<211>  653
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- CCR7-XTEN-FurinSite-GFP

<400>  8

Met Asp Leu Gly Lys Pro Met Lys Ser Val Leu Val Val Ala Leu Leu 
1               5                   10                  15      


Val Ile Phe Gln Val Cys Leu Cys Gln Asp Glu Val Thr Asp Asp Tyr 
            20                  25                  30          


Ile Gly Asp Asn Thr Thr Val Asp Tyr Thr Leu Phe Glu Ser Leu Cys 
        35                  40                  45              


Ser Lys Lys Asp Val Arg Asn Phe Lys Ala Trp Phe Leu Pro Ile Met 
    50                  55                  60                  


Tyr Ser Ile Ile Cys Phe Val Gly Leu Leu Gly Asn Gly Leu Val Val 
65                  70                  75                  80  


Leu Thr Tyr Ile Tyr Phe Lys Arg Leu Lys Thr Met Thr Asp Thr Tyr 
                85                  90                  95      


Leu Leu Asn Leu Ala Val Ala Asp Ile Leu Phe Leu Leu Thr Leu Pro 
            100                 105                 110         


Phe Trp Ala Tyr Ser Ala Ala Lys Ser Trp Val Phe Gly Val His Phe 
        115                 120                 125             


Cys Lys Leu Ile Phe Ala Ile Tyr Lys Met Ser Phe Phe Ser Gly Met 
    130                 135                 140                 


Leu Leu Leu Leu Cys Ile Ser Ile Asp Arg Tyr Val Ala Ile Val Gln 
145                 150                 155                 160 


Ala Val Ser Ala His Arg His Arg Ala Arg Val Leu Leu Ile Ser Lys 
                165                 170                 175     


Leu Ser Cys Val Gly Ile Trp Ile Leu Ala Thr Val Leu Ser Ile Pro 
            180                 185                 190         


Glu Leu Leu Tyr Ser Asp Leu Gln Arg Ser Ser Ser Glu Gln Ala Met 
        195                 200                 205             


Arg Cys Ser Leu Ile Thr Glu His Val Glu Ala Phe Ile Thr Ile Gln 
    210                 215                 220                 


Val Ala Gln Met Val Ile Gly Phe Leu Val Pro Leu Leu Ala Met Ser 
225                 230                 235                 240 


Phe Cys Tyr Leu Val Ile Ile Arg Thr Leu Leu Gln Ala Arg Asn Phe 
                245                 250                 255     


Glu Arg Asn Lys Ala Ile Lys Val Ile Ile Ala Val Val Val Val Phe 
            260                 265                 270         


Ile Val Phe Gln Leu Pro Tyr Asn Gly Val Val Leu Ala Gln Thr Val 
        275                 280                 285             


Ala Asn Phe Asn Ile Thr Ser Ser Thr Cys Glu Leu Ser Lys Gln Leu 
    290                 295                 300                 


Asn Ile Ala Tyr Asp Val Thr Tyr Ser Leu Ala Cys Val Arg Cys Cys 
305                 310                 315                 320 


Val Asn Pro Phe Leu Tyr Ala Phe Ile Gly Val Lys Phe Arg Asn Asp 
                325                 330                 335     


Leu Phe Lys Leu Phe Lys Asp Leu Gly Cys Leu Ser Gln Glu Gln Leu 
            340                 345                 350         


Arg Gln Trp Ser Ser Cys Arg His Ile Arg Arg Ser Ser Met Ser Val 
        355                 360                 365             


Glu Ala Glu Thr Thr Thr Thr Phe Ser Pro Ser Gly Gly Ser Ser Gly 
    370                 375                 380                 


Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro 
385                 390                 395                 400 


Glu Ser Ser Gly Gly Ser Ser Gly Gly Ser Arg Arg Lys Arg Met Val 
                405                 410                 415     


Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu 
            420                 425                 430         


Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly 
        435                 440                 445             


Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr 
    450                 455                 460                 


Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr 
465                 470                 475                 480 


Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His 
                485                 490                 495     


Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr 
            500                 505                 510         


Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys 
        515                 520                 525             


Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp 
    530                 535                 540                 


Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr 
545                 550                 555                 560 


Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile 
                565                 570                 575     


Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln 
            580                 585                 590         


Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val 
        595                 600                 605             


Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys 
    610                 615                 620                 


Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr 
625                 630                 635                 640 


Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
                645                 650             


<210>  9
<211>  1995
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- CCR7-XTEN-FurinSite-NLS-GFP

<400>  9
atggacctgg gcaagcccat gaagtccgtg ctggtggtgg ccctgctggt catctttcag       60

gtgtgcctgt gccaggatga ggtgacagac gattacatcg gcgacaatac cacagtggat      120

tataccctgt tcgagtctct gtgcagcaag aaggacgtgc ggaacttcaa ggcctggttt      180

ctgcctatca tgtacagcat catctgtttc gtgggcctgc tgggcaatgg cctggtggtg      240

ctgacataca tctacttcaa gcggctgaag accatgacag acacctacct gctgaacctg      300

gccgtggccg atatcctgtt cctgctgacc ctgccatttt gggcctattc tgccgccaag      360

agctgggtgt tcggcgtgca cttttgcaag ctgatctttg ccatctacaa gatgtccttc      420

ttttctggca tgctgctgct gctgtgcatc agcatcgaca gatatgtggc aatcgtgcag      480

gccgtgtccg cccaccggca cagagccagg gtgctgctga tcagcaagct gtcctgcgtg      540

ggcatctgga tcctggccac cgtgctgtcc atccctgagc tgctgtactc tgatctgcag      600

agaagctcct ctgagcaggc catgaggtgt tccctgatca cagagcacgt ggaggccttc      660

atcaccatcc aggtggccca gatggtcatc ggcttcctgg tgccactgct ggccatgagc      720

ttttgctacc tggtcatcat cagaaccctg ctgcaggccc gcaactttga gcggaataag      780

gccatcaaag tgatcatcgc cgtggtggtg gtgttcatcg tgtttcagct gccctataat      840

ggcgtggtgc tggcccagac agtggccaac ttcaatatca caagctccac ctgcgagctg      900

tctaagcagc tgaacatcgc ctacgacgtg acctatagcc tggcatgcgt gcggtgctgc      960

gtgaacccct tcctgtatgc ctttatcggc gtgaagtttc gcaatgacct gttcaagctg     1020

tttaaggatc tgggatgcct gagccaggag cagctgaggc agtggtctag ctgtcggcac     1080

atccggagat cctctatgtc cgtggaggcc gagaccacaa ccacattctc tcctagcgga     1140

ggaagctccg gaggatctag cggatctgag acaccaggca cctccgagtc tgccacacca     1200

gagtcctctg gcggcagctc cggaggaagc aggcgcaaga gacccaagaa gaagcggaag     1260

gtgtctggcg gcagcatggt gtccaaggga gaggagctgt tcaccggagt ggtgcctatc     1320

ctggtggagc tggacggcga tgtgaatggc cacaagttta gcgtgtccgg agagggagag     1380

ggcgatgcaa catacggcaa gctgaccctg aagttcatct gcaccacagg caagctgccc     1440

gtgccttggc caacactggt gaccacactg acctacggcg tgcagtgttt ctccaggtat     1500

cctgaccaca tgaagcagca cgatttcttt aagtctgcca tgccagaggg ctacgtgcag     1560

gagcgcacca tcttctttaa ggacgatggc aactataaga cacgggccga ggtgaagttc     1620

gagggcgaca ccctggtgaa cagaatcgag ctgaagggca tcgactttaa ggaggatggc     1680

aatatcctgg gccacaagct ggagtacaac tataattccc acaacgtgta catcatggcc     1740

gataagcaga agaacggcat caaggtgaac ttcaagatca ggcacaatat cgaggacggc     1800

agcgtgcagc tggccgatca ctaccagcag aacacaccaa tcggcgacgg acccgtgctg     1860

ctgcctgata atcactatct gtctacccag agcgccctgt ccaaggaccc caacgagaag     1920

cgcgatcaca tggtgctgct ggagttcgtg acagcagcag gaatcaccct gggaatggac     1980

gagctgtata agtga                                                      1995


<210>  10
<211>  664
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- CCR7-XTEN-FurinSite-NLS-GFP

<400>  10

Met Asp Leu Gly Lys Pro Met Lys Ser Val Leu Val Val Ala Leu Leu 
1               5                   10                  15      


Val Ile Phe Gln Val Cys Leu Cys Gln Asp Glu Val Thr Asp Asp Tyr 
            20                  25                  30          


Ile Gly Asp Asn Thr Thr Val Asp Tyr Thr Leu Phe Glu Ser Leu Cys 
        35                  40                  45              


Ser Lys Lys Asp Val Arg Asn Phe Lys Ala Trp Phe Leu Pro Ile Met 
    50                  55                  60                  


Tyr Ser Ile Ile Cys Phe Val Gly Leu Leu Gly Asn Gly Leu Val Val 
65                  70                  75                  80  


Leu Thr Tyr Ile Tyr Phe Lys Arg Leu Lys Thr Met Thr Asp Thr Tyr 
                85                  90                  95      


Leu Leu Asn Leu Ala Val Ala Asp Ile Leu Phe Leu Leu Thr Leu Pro 
            100                 105                 110         


Phe Trp Ala Tyr Ser Ala Ala Lys Ser Trp Val Phe Gly Val His Phe 
        115                 120                 125             


Cys Lys Leu Ile Phe Ala Ile Tyr Lys Met Ser Phe Phe Ser Gly Met 
    130                 135                 140                 


Leu Leu Leu Leu Cys Ile Ser Ile Asp Arg Tyr Val Ala Ile Val Gln 
145                 150                 155                 160 


Ala Val Ser Ala His Arg His Arg Ala Arg Val Leu Leu Ile Ser Lys 
                165                 170                 175     


Leu Ser Cys Val Gly Ile Trp Ile Leu Ala Thr Val Leu Ser Ile Pro 
            180                 185                 190         


Glu Leu Leu Tyr Ser Asp Leu Gln Arg Ser Ser Ser Glu Gln Ala Met 
        195                 200                 205             


Arg Cys Ser Leu Ile Thr Glu His Val Glu Ala Phe Ile Thr Ile Gln 
    210                 215                 220                 


Val Ala Gln Met Val Ile Gly Phe Leu Val Pro Leu Leu Ala Met Ser 
225                 230                 235                 240 


Phe Cys Tyr Leu Val Ile Ile Arg Thr Leu Leu Gln Ala Arg Asn Phe 
                245                 250                 255     


Glu Arg Asn Lys Ala Ile Lys Val Ile Ile Ala Val Val Val Val Phe 
            260                 265                 270         


Ile Val Phe Gln Leu Pro Tyr Asn Gly Val Val Leu Ala Gln Thr Val 
        275                 280                 285             


Ala Asn Phe Asn Ile Thr Ser Ser Thr Cys Glu Leu Ser Lys Gln Leu 
    290                 295                 300                 


Asn Ile Ala Tyr Asp Val Thr Tyr Ser Leu Ala Cys Val Arg Cys Cys 
305                 310                 315                 320 


Val Asn Pro Phe Leu Tyr Ala Phe Ile Gly Val Lys Phe Arg Asn Asp 
                325                 330                 335     


Leu Phe Lys Leu Phe Lys Asp Leu Gly Cys Leu Ser Gln Glu Gln Leu 
            340                 345                 350         


Arg Gln Trp Ser Ser Cys Arg His Ile Arg Arg Ser Ser Met Ser Val 
        355                 360                 365             


Glu Ala Glu Thr Thr Thr Thr Phe Ser Pro Ser Gly Gly Ser Ser Gly 
    370                 375                 380                 


Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro 
385                 390                 395                 400 


Glu Ser Ser Gly Gly Ser Ser Gly Gly Ser Arg Arg Lys Arg Pro Lys 
                405                 410                 415     


Lys Lys Arg Lys Val Ser Gly Gly Ser Met Val Ser Lys Gly Glu Glu 
            420                 425                 430         


Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val 
        435                 440                 445             


Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr 
    450                 455                 460                 


Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro 
465                 470                 475                 480 


Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly Val Gln Cys 
                485                 490                 495     


Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser 
            500                 505                 510         


Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp 
        515                 520                 525             


Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr 
    530                 535                 540                 


Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly 
545                 550                 555                 560 


Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val 
                565                 570                 575     


Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys 
            580                 585                 590         


Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr 
        595                 600                 605             


Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn 
    610                 615                 620                 


His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys 
625                 630                 635                 640 


Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr 
                645                 650                 655     


Leu Gly Met Asp Glu Leu Tyr Lys 
            660                 


<210>  11
<211>  1047
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- TfnR-XTEN-FurinSite-GFP

<400>  11
atgatggtgg acggcgataa tagccacgtg gagatgaagc tggccgtgga cgaggaggag       60

aacgccgata acaataccaa ggccaatgtg acaaagccta agcggtgctc cggctctatc      120

tgttacggca ccatcgccgt gatcgtgttc tttctgatcg gctttatgat cggctacctg      180

ggctattgca agagctccga cggaccagga gagaccggat ccggaggctc tagcggagga      240

tcctctggat ctgagacccc aggcacaagc gagtccgcca caccagagag ctccggagga      300

tctagcggag gatcccggag aaagaggatg gtgagcaagg gcgaggagct gttcacaggc      360

gtggtgccta tcctggtgga gctggacggc gatgtgaatg gccacaagtt ttctgtgagc      420

ggagagggag agggcgatgc aacctatggc aagctgacac tgaagttcat ctgcaccaca      480

ggcaagctgc ccgtgccttg gccaaccctg gtgaccacac tgacatacgg cgtgcagtgt      540

ttcagcagat atcctgacca catgaagcag cacgatttct ttaagtccgc catgccagag      600

ggctacgtgc aggagcggac catcttcttt aaggacgatg gcaactataa gaccagagcc      660

gaggtgaagt tcgagggcga cacactggtg aacaggatcg agctgaaggg catcgacttt      720

aaggaggatg gcaatatcct gggccacaag ctggagtaca actataattc tcacaacgtg      780

tacatcatgg ccgataagca gaagaacggc atcaaggtga acttcaagat ccgccacaat      840

atcgaggacg gctccgtgca gctggccgat cactaccagc agaacacccc aatcggcgac      900

ggacccgtgc tgctgcctga taatcactat ctgtccacac agtctgccct gagcaaggac      960

cccaacgaga agcgggatca catggtgctg ctggagttcg tgaccgcagc aggaatcaca     1020

ctgggaatgg acgagctgta taagtga                                         1047


<210>  12
<211>  348
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- TfnR-XTEN-FurinSite-GFP

<400>  12

Met Met Val Asp Gly Asp Asn Ser His Val Glu Met Lys Leu Ala Val 
1               5                   10                  15      


Asp Glu Glu Glu Asn Ala Asp Asn Asn Thr Lys Ala Asn Val Thr Lys 
            20                  25                  30          


Pro Lys Arg Cys Ser Gly Ser Ile Cys Tyr Gly Thr Ile Ala Val Ile 
        35                  40                  45              


Val Phe Phe Leu Ile Gly Phe Met Ile Gly Tyr Leu Gly Tyr Cys Lys 
    50                  55                  60                  


Ser Ser Asp Gly Pro Gly Glu Thr Gly Ser Gly Gly Ser Ser Gly Gly 
65                  70                  75                  80  


Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu 
                85                  90                  95      


Ser Ser Gly Gly Ser Ser Gly Gly Ser Arg Arg Lys Arg Met Val Ser 
            100                 105                 110         


Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu 
        115                 120                 125             


Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu 
    130                 135                 140                 


Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr 
145                 150                 155                 160 


Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr 
                165                 170                 175     


Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp 
            180                 185                 190         


Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile 
        195                 200                 205             


Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe 
    210                 215                 220                 


Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe 
225                 230                 235                 240 


Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn 
                245                 250                 255     


Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys 
            260                 265                 270         


Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu 
        275                 280                 285             


Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu 
    290                 295                 300                 


Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp 
305                 310                 315                 320 


Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala 
                325                 330                 335     


Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
            340                 345             


<210>  13
<211>  1080
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- TfnR(TM)-linker (XTEN)-FurinSite-NLS-GFP

<400>  13
atgatggtgg acggcgataa tagccacgtg gagatgaagc tggccgtgga cgaggaggag       60

aacgccgata acaataccaa ggccaatgtg acaaagccta agcggtgctc cggctctatc      120

tgttacggca ccatcgccgt gatcgtgttc tttctgatcg gctttatgat cggctacctg      180

ggctattgca agagctccga cggaccagga gagaccggat ccggaggctc tagcggagga      240

tcctctggat ctgagacccc aggcacaagc gagtccgcca caccagagag ctccggagga      300

tctagcggag gatcccggag aaagaggccc aagaagaagc ggaaggtgtc cggcggctct      360

atggtgagca agggcgagga gctgttcaca ggcgtggtgc ctatcctggt ggagctggac      420

ggcgatgtga atggccacaa gttttctgtg agcggagagg gagagggcga tgcaacctat      480

ggcaagctga cactgaagtt catctgcacc acaggcaagc tgcccgtgcc ttggccaacc      540

ctggtgacca cactgacata cggcgtgcag tgtttcagca gatatcctga ccacatgaag      600

cagcacgatt tctttaagtc cgccatgcca gagggctacg tgcaggagcg gaccatcttc      660

tttaaggacg atggcaacta taagaccaga gccgaggtga agttcgaggg cgacacactg      720

gtgaacagga tcgagctgaa gggcatcgac tttaaggagg atggcaatat cctgggccac      780

aagctggagt acaactataa ttctcacaac gtgtacatca tggccgataa gcagaagaac      840

ggcatcaagg tgaacttcaa gatccgccac aatatcgagg acggctccgt gcagctggcc      900

gatcactacc agcagaacac cccaatcggc gacggacccg tgctgctgcc tgataatcac      960

tatctgtcca cacagtctgc cctgagcaag gaccccaacg agaagcggga tcacatggtg     1020

ctgctggagt tcgtgaccgc agcaggaatc acactgggaa tggacgagct gtataagtga     1080


<210>  14
<211>  359
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- TfnR(TM)-linker (XTEN)-FurinSite-NLS-GFP

<400>  14

Met Met Val Asp Gly Asp Asn Ser His Val Glu Met Lys Leu Ala Val 
1               5                   10                  15      


Asp Glu Glu Glu Asn Ala Asp Asn Asn Thr Lys Ala Asn Val Thr Lys 
            20                  25                  30          


Pro Lys Arg Cys Ser Gly Ser Ile Cys Tyr Gly Thr Ile Ala Val Ile 
        35                  40                  45              


Val Phe Phe Leu Ile Gly Phe Met Ile Gly Tyr Leu Gly Tyr Cys Lys 
    50                  55                  60                  


Ser Ser Asp Gly Pro Gly Glu Thr Gly Ser Gly Gly Ser Ser Gly Gly 
65                  70                  75                  80  


Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu 
                85                  90                  95      


Ser Ser Gly Gly Ser Ser Gly Gly Ser Arg Arg Lys Arg Pro Lys Lys 
            100                 105                 110         


Lys Arg Lys Val Ser Gly Gly Ser Met Val Ser Lys Gly Glu Glu Leu 
        115                 120                 125             


Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn 
    130                 135                 140                 


Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr 
145                 150                 155                 160 


Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val 
                165                 170                 175     


Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly Val Gln Cys Phe 
            180                 185                 190         


Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala 
        195                 200                 205             


Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp 
    210                 215                 220                 


Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu 
225                 230                 235                 240 


Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn 
                245                 250                 255     


Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr 
            260                 265                 270         


Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys Ile 
        275                 280                 285             


Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln 
    290                 295                 300                 


Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His 
305                 310                 315                 320 


Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg 
                325                 330                 335     


Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu 
            340                 345                 350         


Gly Met Asp Glu Leu Tyr Lys 
        355                 


<210>  15
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic- MS2 loop

<400>  15
acatgaggat cacccatgt                                                    19


<210>  16
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- TALE binding motif

<400>  16

Leu Thr Pro Glu Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys 
1               5                   10                  15      


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Arg Val Cys Gln Ala His 
            20                  25                  30          


Gly 
    


<210>  17
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic- HUH protein tag

<400>  17

Met Ala Lys Ser Gly Asn Tyr Ser Tyr Lys Arg Trp Val Phe Thr Ile 
1               5                   10                  15      


Asn Asn Pro Thr Phe Glu Asp Tyr Val His Val Leu Glu Phe Cys Thr 
            20                  25                  30          


Leu Asp Asn Cys Lys Phe Ala Ile Val Gly Glu Glu Lys Gly Ala Asn 
        35                  40                  45              


Gly Thr Pro His Leu Gln Gly Phe Leu Asn Leu Arg Ser Asn Ala Arg 
    50                  55                  60                  


Ala Ala Ala Leu Glu Glu Ser Leu Gly Gly Arg Ala Trp Leu Ser Arg 
65                  70                  75                  80  


Ala Arg Gly Ser Asp Glu Asp Asn Glu Glu Tyr Cys Ala Lys Glu Ser 
                85                  90                  95      


Thr Tyr Leu Arg Val Gly Glu Pro Val Ser Lys Gly Arg Ser Ser 
            100                 105                 110     


