                         SEQUENCE LISTING

<110>  President and Fellows of Harvard College
 
<120>  METHODS FOR MAKING AND USING GENOMICALLY RECODED CELLS

<130>  010498.01448

<140>  63/122,426
<141>  2020-12-07

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  11
<212>  PRT
<213>  Artificial

<220>
<223>  C terminal degradation tag

<400>  1

Ala Ala Asn Asp Glu Asn Tyr Ala Leu Ala Ala 
1               5                   10      


<210>  2
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  I SceI cleaved base pair sequence

<400>  2
tagggataac agggtaat                                                     18


<210>  3
<211>  4129
<212>  DNA
<213>  Artificial

<220>
<223>  pCTXVP60

<400>  3
gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt       60

gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt      120

ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga      180

tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga      240

aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt      300

ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc      360

ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat      420

ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt      480

gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg      540

acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact      600

ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa      660

aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc      720

actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc      780

ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa      840

agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc      900

agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc      960

tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc     1020

gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac     1080

tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt     1140

gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt     1200

agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg     1260

tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt     1320

cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc     1380

aaaacgatct caagaagatc atcttattag gatctattaa tacgactcac tatagggatc     1440

ccgggcgcgc cgtcgacttt aaagttcatc cttttcagaa acatatgaaa atccattagc     1500

agttcctcca agattaaaaa caagagtaga cttagatggt cttggtccaa ctggtctaac     1560

atcaataagt tcagtaagat caataagagt agtagaagct ccagttccag cataaaaata     1620

tccatcaaca gaaagtccaa tttccatgaa tccagaagca aaagtaagtt gccaaacaaa     1680

aaattgtcct ggcataagag cagaagaata attattaaga gaaagtccaa tagtaactgg     1740

aagtggttga gatccagttc catattgagt tccattagca gatccagcag tagcattaac     1800

atctccagtt cttctaacaa cagaagcaaa cataattgga gtattctttc caactggagc     1860

tgcagctgga gttcctggag tagtaacaat tctatctggt tgtggagtat aagtaatagc     1920

attagcagat ggagtagaaa taactccaga agccataaca aaaagtccag ctggattttg     1980

agcagttcca gtaacaacag catagataga cttagcaaca gtttgagatc cagaagtatt     2040

agtagttggt tgaagatttc ctggagctcc agtagcaaat ccaagttcat aagcttgaac     2100

agtagtaaca tttggagctc cagaattaga gttccaaata gctccaaatc caacccatcc     2160

agcagctgga atacctggtc cattgaatgg aacaaaagac atatctggaa atccatctgg     2220

agcaacttga gaaattggat tatcaatagc agatccagca ttagcatacc aaaattgaag     2280

aacattagta gcattagatc ctggataaga agcagatcct cttctatgat cgatatcagc     2340

aaatcttgga gaagaccatc cataagtaga tccattaaga ttccaatgtc tattacaagt     2400

agaaaatcct cctggaactg gttgaagtcc aacaatttgt ccattccatc tattatcatt     2460

tccaactcca gtaagaactg gagtagtaag aagtccagct ggatagatag aatcaacagt     2520

cttagaagat ggagttctaa tcataacaaa ttcaaaatct tcagatggtc tagtttcaac     2580

agtaacttga atagcagaag tagatcctcc aaatggatta ataagattat tataaacaga     2640

aagaacaaga gttggaacaa gtcctggatc tccagttgga tgatacatat ttggtctaag     2700

gtctggcata gtaatagtaa ctggttcaag agacctagca tcaataacaa catgtggaaa     2760

ttgtctaact tcaagacctg gtccaatttc aattcctggt ggaataacag cagcaacaag     2820

tcttcctcca aaaactccag atccagcaac aatgaatctg aattgcattc ctccagccca     2880

tccagcatac atttgagaaa gaacagcagt gaatggatta ttttgtggag aatgttgaac     2940

agtataaaga atagatcctg gagcatcagc aacagaccaa gtaaaaacat cattataata     3000

aaaattagtt ctccaagttt cttgttgatc aacttgttgt ggtggtcctc caattccagc     3060

agtagcaata gaagcagaag aattttcagc agtaacaaca gaagtagtag caacaactcc     3120

tgggtccatt ccatcagtag tagttcctgg aacagaagca gtagtagcag ttccagcagc     3180

tccagcttgt ggagcagttc tagcctttcc ttccatgggt cctggtccat tagccataga     3240

aatagcagca atagcatgtg gagtcttatt attccaaaca cacaactttt caaccttagc     3300

ttcagtaaga taagcaattc taagagtatc cttcattctt tcaatagcct tcttttgaga     3360

atcaatatgt tgagatcctg gaacttcaac ttgaaaagta gctccattct taaaagtaat     3420

aatagccatt tctctctttc cagcaagaga ttcagtataa gaaaaaatct tatcattaag     3480

agtatgaatt tgagtattat gatattcagc acaaagatca gtaatatttt gtggagtgga     3540

ggaagccatt gtcccgggcg gccgcttcta tagtgtcacc taaatcgggt cgaatttgct     3600

ttcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtacaa ccaggcgttt     3660

aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca tcgcagtact     3720

gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct     3780

gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atagtgaaaa     3840

cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc     3900

agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt     3960

tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt     4020

ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag     4080

ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacg                 4129


<210>  4
<211>  5621
<212>  DNA
<213>  Artificial

<220>
<223>  ptRNAsup plasmid

<400>  4
tcattagtct tcaatgacgt gtaaaccacg gcgctttaag tcctctaacg aatccaacat       60

tccccttatt aattcaacag gatgcccctc ccagtcttca acaacgccaa caattctcaa      120

gggttcgcag gttctatagg actgtgttgg attaccggga aattttttgt tcgtaagatt      180

cggatcgtct tcgaacggtc ctgttggctc aactatgtat atgtagccgc gaccctcgag      240

gccagacagt gacatagcaa gttcagctcc ccaaactgct ggctccatca aggctgaaaa      300

gtagatgtgc ttaagaatac gaccgtcctc gaaatgagag atgaaccctg tggttagcaa      360

gtcaccaatc gccaaattgg ctttggttcc atgatagaac ggtccttgca cctgcttgta      420

attatcatga gagatgggaa tccaatcttt taccatataa cgaacctcct cttgcttatt      480

acagcaacgc cgcgtctgaa ttatttctag agaagcattt atcagggtta ttgtctcatg      540

agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt      600

ccccgaaaag tgcgagtatt tacaacgaag acgcaatcac tttcttgatc agtgatcgtt      660

tcggcgtaat tttcagcaaa cgatcaaaag tggtgaaaaa tatcgttgac tcatcgcgcc      720

aggtaagtag aatgcaacgc atcgaacggc ggcactgatt gccagacgat aataaaatca      780

agtgattaac tgattgcttg atgaatcttt cttcccgagc ccggatggtg gaatcggtag      840

acacaaggga tttaaaatcc ctcggcgttc gcgctgtgcg ggttcaagtc ccgctccggg      900

taccatggga aagataagaa taaaatcaaa gcaataagca gtgtcctcgg taccaaattt      960

tcgaaaaaag acgctgaaaa gcgtcttttt tcgttttggt ccttacaatc agcagtcaga     1020

acttttacga agaatagtgg tcgctcaacc ttttctgttg ataaaacact ctttttgacg     1080

tttttacaga ctaattgaac gtgaagtgtg caaacgataa aagtgtagga aaaattgttt     1140

gacttataag tctcagaaag taatatgtgc gcgacgcagc gacgatgagc gataaacaag     1200

ttcttcgaag cactcgtaag aggcgtgtgg tgaggtggcc gagaggctga aggcgctccc     1260

ctgctaaggg agtatgcggt caaaagctgc atccggggtt cgaatccccg cctcaccgcc     1320

atttgcatct aaaaaaaaac cccgcccctg acagggcggg gttttttttt taattatagg     1380

gataacaggg taatatattc acttccctca cagattcgtt cagagataaa agcgttggta     1440

acagttgcct ggagtgtgac aaagcgttac acatcgctgt atgcaatgct gaaaatttca     1500

gcacttagcg aggtgcgagc aagctggcgc ttgcatggtg gcgtgcgaca ggtataatcc     1560

acaacgtttt cgcgcatacc tcttcagtgc cgaagtggcg aaatcggtag acgcagttga     1620

ttcaaaatca accgtagaaa tacgtgccgg ttcgagtccg gccttcggca ccaaaagtat     1680

gtaaatagac ctcaactgag gtcttttttt atgccaatta ttgaaggccg ctaacgcggc     1740

ctttttttgt ttctggtctc cctgcggata aagttgatac ccttacctga gttcttctga     1800

aaataacgga ctaaaaagca gaacgtgcgc gaaaacatta agaaaaatta taaaaacccg     1860

gcataaatgg cgagggttta agcaatcgag cggcagcgta cttaccccgc actcgattag     1920

cgggtatact catgccgcat tgtcctctta gttaaatgga tataacgagc ccctcctaag     1980

ggctaattgc aggttcgatt cctgcagggg acaccattta tcagttcgct cccatccgta     2040

ccagtccgca aaatccaacg catgagaaag cccccggaag atcaccttcc gggggctttt     2100

ttattgcgct gaaaagcaat ccctcgtgaa gtaactcaat agtgttctct ggtatcgtag     2160

tgtgcgtttg tttgccgcta tagcgaaata aatcagaaaa tcagacgcgg tcgttcactt     2220

gttcagcaac cagatcaaaa gccattgact cagcaagcgt tgaccgtata attcacgcga     2280

ttacacgcgc attgcggtat caacgcgccc ttagctcagt tggatagagc aacgaccttc     2340

taagtcgtgg gccgcaggtt cgaatcctgc agggcgcgcc attacaattc aatcagttac     2400

gccttcttta tatcctccat aagaaaaaag cccgcacctg acagtgcggg cttttttttt     2460

cgatcacttc tggaagcttg agcacgtgtt gacaattaat catcggcata gtatatcggc     2520

atagtataat acgacaaggt gaggaactaa acccctataa cccactaaga agaatttatg     2580

tcacatctcg cagaactggt tgcctcagcg aaggcggcca tttcacaggc gtcagatgtt     2640

gccgcgcttg ataatgtgcg cgtcgaatat ctaggtaaaa aagggcacct aacccttcag     2700

atgacgaccc tgcgtgagct gccgccagaa gagcgtccgg cagctggtgc ggttatcaac     2760

gaagcgaaag agcaggttca gcaggcgctg aatgcgcgta aagcggaact ggaatccgct     2820

gcactgaatg cgcgtctggc ggcggaaacg attgatgtct ctctgccagg tcgtcgcatt     2880

gaaaacggcg gtctgcatcc ggttacccgt accatcgacc gtatcgaatc attcttcggt     2940

gagcttggct ttaccgtggc aaccgggccg gaaatcgaag acgattatca taacttcgat     3000

gctctgaaca ttcctggtca ccacccggcg cgagctgacc acgacacttt ctggtttgac     3060

actacccgcc tgctgcgtac ccagacctct ggcgtacaga tccgcaccat gaaagcccag     3120

cagccaccga ttcgtatcat cgcgcctggc cgtgtttatc gtaacgacta cgaccagact     3180

cacacgccga tgttccatca gatggaaggt ctgattgttg ataccaacat ctcctttacc     3240

aacctgaaag gcacgctgca cgacttcctg cgtaacttct ttgaggaaga tctccagatt     3300

cgcttccgtc cttcctactt cccgtttgcc gaaccttctg cagaagtgga cgtcatgggt     3360

aaaaacggta aatggctgga agtgctgggc tgcgggatgg tgcatccgaa cgtgcttcgt     3420

aacgttggca tcgacccgga agtttactct ggtttcggct tcgggatggg gatggagcgt     3480

ctgactatgc tacgttacgg cgtcaccgac ctgcgttcat tcttcgaaaa cgatctgcgt     3540

ttcctcaaac agtttaaata acattaccct gttatcccta aataagattt acggattact     3600

atcttttcga aaaaaggcct cccaaatcgg ggggcctttt ttattgataa caaaatccat     3660

gggtatggac agttttccct ttgatatgta acggtgaaca gttgttctac ttttgtttgt     3720

tagtcttgat gcttcactga tagatacaag agccataaga acctcagatc cttccgtatt     3780

tagccagtat gttctctagt gtggttcgtt gtttttgcgt gagccatgag aacgaaccat     3840

tgagatcata cttactttgc atgtcactca aaaattttgc ctcaaaactg gtgagctgaa     3900

tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc gttacgtagg taggaatctg     3960

atgtaatggt tgttggtatt ttgtcaccat tcatttttat ctggttgttc tcaagttcgg     4020

ttacgagatc catttgtcta tctagttcaa cttggaaaat caacgtatca gtcgggcggc     4080

ctcgcttatc aaccaccaat ttcatattgc tgtaagtgtt taaatcttta cttattggtt     4140

tcaaaaccca ttggttaagc cttttaaact catggtagtt attttcaagc attaacatga     4200

acttaaattc atcaaggcta atctctatat ttgccttgtg agttttcttt tgtgttagtt     4260

cttttaataa ccactcataa atcctcatag agtatttgtt ttcaaaagac ttaacatgtt     4320

ccagattata ttttatgaat ttttttaact ggaaaagata aggcaatatc tcttcactaa     4380

aaactaattc taatttttcg cttgagaact tggcatagtt tgtccactgg aaaatctcaa     4440

agcctttaac caaaggattc ctgatttcca cagttctcgt catcagctct ctggttgctt     4500

tagctaatac accataagca ttttccctac tgatgttcat catctgagcg tattggttat     4560

aagtgaacga taccgtccgt tctttccttg tagggttttc aatcgtgggg ttgagtagtg     4620

ccacacagca taaaattagc ttggtttcat gctccgttaa gtcatagcga ctaatcgcta     4680

gttcatttgc tttgaaaaca actaattcag acatacatct caattggtct aggtgatttt     4740

aatcactata ccaattgaga tgggctagtc aatgataatt actagtcctt ttcctttgag     4800

ttgtgggtat ctgtaaattc tgctagacct ttgctggaaa acttgtaaat tctgctagac     4860

cctctgtaaa ttccgctaga cctttgtgtg ttttttttgt ttatattcaa gtggttataa     4920

tttatagaat aaagaaagaa taaaaaaaga taaaaagaat agatcccagc cctgtgtata     4980

actcactact ttagtcagtt ccgcagtatt acaaaaggat gtcgcaaacg ctgtttgctc     5040

ctctacaaaa cagaccttaa aaccctaaag gcttaagtag caccctcgca agctcggttg     5100

cggccgcaat cgggcaaatc gctgaatatt ccttttgtct ccgaccatca ggcacctgag     5160

tcgctgtctt tttcgtgaca ttcagttcgc tgcgctcacg gctctggcag tgaatggggg     5220

taaatggcac tacaggcgcc ttttatggat tcatgcaagg aaactaccca taatacaaga     5280

aaagcccgtc acgggcttct cagggcgttt tatggcgggt ctgctatgtg gtgctatctg     5340

actttttgct gttcagcagt tcctgccctc tgattttcca gtctgaccac ttcggattat     5400

cccgtgacag gtcattcaga ctggctaatg cacccagtaa ggcagcggta tcatcaacgg     5460

ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa     5520

aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta     5580

tatatgagta aacttggtct gactagggat aacagggtaa t                         5621


<210>  5
<211>  717
<212>  DNA
<213>  Artificial

<220>
<223>  chimeric construct CTXVP60 orf238

<400>  5
atgctattgc tgctatttct atggctaatg gaccaggacc catggaagga aaggctagaa       60

ctgctccaca agctggagct gctggaactg ctactactgc ttctgttcca ggaactacta      120

ctgatggaat ggacccagga gttgttgcta ctacttctgt tgttactgct gaaaattctt      180

ctgcttctat tgctactgct ggaattggag gaccaccaca acaagttgat caacaagaaa      240

cttggagaac taatttttat tataatgatg tttttacttg gtctgttgct gatgctccag      300

gatctattct ttatactgtt caacattctc cacaaaataa tccattcact gctgttcttt      360

ctcaaatgta tgctggatgg gctggaggaa tgcaattcag attcattgtt gctggatctg      420

gagtttttgg aggaagactt gttgctgctg ttattccacc aggaattgaa attggaccag      480

gtcttgaagt tagacaattt ccacatgttg ttattgatgc taggtctctt gaaccagtta      540

ctattactat gccagacctt agaccaaata tgtatcatcc aactggagat ccaggacttg      600

ttccaactct tgttctttct gtttataata atcttattaa tccatttgga ggatctactt      660

ctgctattca agttactgtt gaaactagac catctgaaga ttttgaattt gttatga         717


<210>  6
<211>  1917
<212>  DNA
<213>  Artificial

<220>
<223>  human growth hormone plasmid

<400>  6
atggatctct ggcagctgct gttgaccttg gcactggcag gatcaagtga tgctttttct       60

ggaagtgagg ccacagcagc tatccttagc agagcaccct ggagtctgca aagtgttaat      120

ccaggcctaa agacaaattc ttctaaggag cctaaattca ccaagtgccg ttcacctgag      180

cgagagactt tttcatgcca ctggacagat gaggttcatc atggtacaaa gaacctagga      240

cccatacagc tgttctatac cagaaggaac actcaagaat ggactcaaga atggaaagaa      300

tgccctgatt atgtttctgc tggggaaaac agctgttact ttaattcatc gtttacctcc      360

atctggatac cttattgtat caagctaact agcaatggtg gtacagtgga tgaaaagtgt      420

ttctctgttg atgaaatagt gcaaccagat ccacccattg ccctcaactg gactttactg      480

aacgtcagtt taactgggat tcatgcagat atccaagtga gatgggaagc accacgcaat      540

gcagatattc agaaaggatg gatggttctg gagtatgaac ttcaatacaa agaagtaaat      600

gaaactaaat ggaaaatgat ggaccctata ttgacaacat cagttccagt gtactcattg      660

aaagtggata aggaatatga agtgcgtgtg agatccaaac aacgaaactc tggaaattat      720

ggcgagttca gtgaggtgct ctatgtaaca cttcctcaga tgagccaatt tacatgtgaa      780

gaagatttct actttccatg gctcttaatt attatctttg gaatatttgg gctaacagtg      840

atgctatttg tattcttatt ttctaaacag caaaggatta aaatgctgat tctgccccca      900

gttccagttc caaagattaa aggaatcgat ccagatctcc tcaaggaagg aaaattagag      960

gaggtgaaca caatcttagc cattcatgat agctataaac ccgaattcca cagtgatgac     1020

tcttgggttg aatttattga gctagatatt gatgagccag atgaaaagac tgaggaatca     1080

gacacagaca gacttctaag cagtgaccat gagaaatcac atagtaacct aggggtgaag     1140

gatggcgact ctggacgtac cagctgttgt gaacctgaca ttctggagac tgatttcaat     1200

gccaatgaca tacatgaggg tacctcagag gttgctcagc cacagaggtt aaaaggggaa     1260

gcagatctct tatgccttga ccagaagaat caaaataact caccttatca tgatgcttgc     1320

cctgctactc agcagcccag tgttatccaa gcagagaaaa acaaaccaca accacttcct     1380

actgaaggag ctgagtcaac tcaccaagct gcccatattc agctaagcaa tccaagttca     1440

ctgtcaaaca tcgactttta tgcccaggtg agcgacatta caccagcagg tagtgtggtc     1500

ctttccccgg gccaaaagaa taaggcaggg atgtcccaat gtgacatgca cccggaaatg     1560

gtctcactct gccaagaaaa cttccttatg gacaatgcct acttctgtga ggcagatgcc     1620

aaaaagtgca tccctgtggc tcctcacatc aaggttgaat cacacataca gccaagctta     1680

aaccaagagg acatttacat caccacagaa agccttacca ctgctgctgg gaggcctggg     1740

acaggagaac atgttccagg ttctgagatg cctgtcccag actatacctc cattcatata     1800

gtacagtccc cacagggcct catactcaat gcgactgcct tgcccttgcc tgacaaagag     1860

tttctctcat catgtggcta tgtgagcaca gaccaactga acaaaatcat gccttag        1917


<210>  7
<211>  4814
<212>  DNA
<213>  Artificial

<220>
<223>  pUC-hGHR wt-RC55 plasmid

<400>  7
ttatattatt tttgccaaat aatttttaac aaaagctctg aagtcttctt catttaaatt       60

cttagatgat acttcatctg gaaaattgtc ccaattagta gcatcacgct gtgagtaagt      120

tctaaaccat ttttttattg ttgtattatc tctaatctta ctactcgatg agttttcggt      180

attatctcta tttttaactt ggagcaggtt ccattcattg tttttttcat catagtgaat      240

aaaatcaact gctttaacac ttgtgcctga acaccatatc catccggcgt aatacgactc      300

actataggga gagcggccgc cagatcttcc ggatggctcg agtttttcag caagatatgg      360

atctctggca gctgctgttg accttggcac tggcaggatc aagtgatgct ttttctggaa      420

gtgaggccac agcagctatc cttagcagag caccctggag tctgcaaagt gttaatccag      480

gcctaaagac aaattcttct aaggagccta aattcaccaa gtgccgttca cctgagcgag      540

agactttttc atgccactgg acagatgagg ttcatcatgg tacaaagaac ctaggaccca      600

tacagctgtt ctataccaga aggaacactc aagaatggac tcaagaatgg aaagaatgcc      660

ctgattatgt ttctgctggg gaaaacagct gttactttaa ttcatcgttt acctccatct      720

ggatacctta ttgtatcaag ctaactagca atggtggtac agtggatgaa aagtgtttct      780

ctgttgatga aatagtgcaa ccagatccac ccattgccct caactggact ttactgaacg      840

tcagtttaac tgggattcat gcagatatcc aagtgagatg ggaagcacca cgcaatgcag      900

atattcagaa aggatggatg gttctggagt atgaacttca atacaaagaa gtaaatgaaa      960

ctaaatggaa aatgatggac cctatattga caacatcagt tccagtgtac tcattgaaag     1020

tggataagga atatgaagtg cgtgtgagat ccaaacaacg aaactctgga aattatggcg     1080

agttcagtga ggtgctctat gtaacacttc ctcagatgag ccaatttaca tgtgaagaag     1140

atttctactt tccatggctc ttaattatta tctttggaat atttgggcta acagtgatgc     1200

tatttgtatt cttattttct aaacagcaaa ggattaaaat gctgattctg cccccagttc     1260

cagttccaaa gattaaagga atcgatccag atctcctcaa ggaaggaaaa ttagaggagg     1320

tgaacacaat cttagccatt catgatagct ataaacccga attccacagt gatgactctt     1380

gggttgaatt tattgagcta gatattgatg agccagatga aaagactgag gaatcagaca     1440

cagacagact tctaagcagt gaccatgaga aatcacatag taacctaggg gtgaaggatg     1500

gcgactctgg acgtaccagc tgttgtgaac ctgacattct ggagactgat ttcaatgcca     1560

atgacataca tgagggtacc tcagaggttg ctcagccaca gaggttaaaa ggggaagcag     1620

atctcttatg ccttgaccag aagaatcaaa ataactcacc ttatcatgat gcttgccctg     1680

ctactcagca gcccagtgtt atccaagcag agaaaaacaa accacaacca cttcctactg     1740

aaggagctga gtcaactcac caagctgccc atattcagct aagcaatcca agttcactgt     1800

caaacatcga cttttatgcc caggtgagcg acattacacc agcaggtagt gtggtccttt     1860

ccccgggcca aaagaataag gcagggatgt cccaatgtga catgcacccg gaaatggtct     1920

cactctgcca agaaaacttc cttatggaca atgcctactt ctgtgaggca gatgccaaaa     1980

agtgcatccc tgtggctcct cacatcaagg ttgaatcaca catacagcca agcttaaacc     2040

aagaggacat ttacatcacc acagaaagcc ttaccactgc tgctgggagg cctgggacag     2100

gagaacatgt tccaggttct gagatgcctg tcccagacta tacctccatt catatagtac     2160

agtccccaca gggcctcata ctcaatgcga ctgccttgcc cttgcctgac aaagagtttc     2220

tctcatcatg tggctatgtg agcacagacc aactgaacaa aatcatgcct tagatctttc     2280

tagaagatct cctacaatat tctcagctgc catggaaaat cgatgttctt cttttattct     2340

ctcaagattt tcaggctgta tattaaaact tatattaaga actatgctaa ccacctcatc     2400

aggaaccgtt gtaggtggcg tgggttttct tggcaatcga ctctcatgaa aactacgagc     2460

taaatattca atatgttcct cttgaccaac tttattctgc attttttttg aacgaggttt     2520

agagcaagct tcaggaaact gagacaggaa ttttattaaa aatttaaatt ttgaagaaag     2580

ttcagggtta atagcatcca ttttttgctt tgcaagttcc tcagcattct taacaaaaga     2640

cgtctctttt gacatgttta aagtttaaac ctcctgtgtg aaattgttat ccgctcacaa     2700

ttccacacat tatacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga     2760

gctaactcac attaattgcg ttgcgctcac tgccaattgc tttccagtcg ggaaacctgt     2820

cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc     2880

gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg     2940

tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa     3000

agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg     3060

cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga     3120

ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg     3180

tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg     3240

gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc     3300

gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg     3360

gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca     3420

ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt     3480

ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag     3540

ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg     3600

gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc     3660

ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt     3720

tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt     3780

ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca     3840

gagaggcacc tatctcagcg atctggcgat ttcgttcatc catagttgcc tgggaccccg     3900

tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac     3960

cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg     4020

cggagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatc agttgttgcc     4080

gggaagctag agtaagtagt tcgccagtta gtagtttgcg cagcgttgtt gccattgcta     4140

caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac     4200

gatcaaggcg agttacatga tcccccatgt tgtgcagaaa agcggttagc tccttcggtc     4260

ctccgatcgt tgtcagaagc aggttggccg cagtgttatc agacatggtt atggcagcag     4320

agcacagttc gcgtactgtc atgccatccg taagatgctt ttctgtgact ggggagtact     4380

caacgaggtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa     4440

tacgggacag taccgcgcca catagcagaa ctttaaaagt ggacatcatt ggaaaacgtt     4500

cttcggggcg aaaggactca aggatcttac cagagttgag atccagttcg atgtaaccca     4560

ctcgtgcacc tagctgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa     4620

aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatgg     4680

acatcgtagg gtaacctgaa tattattgaa gcatttatca gggttattgt ctcatgagcg     4740

gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc     4800

gaaaagtgcc acct                                                       4814


<210>  8
<211>  374
<212>  DNA
<213>  Artificial

<220>
<223>  Hok expression construct

<400>  8
cacagctaac accacgtcgt ccctatctgc tgccctaggt ctatgagtgg ttgctggata       60

actttacggg catgcataag gctcgtatga tatattcagg gagaccacaa cggtttccct      120

ctacaaataa ttttgtttaa ctttcgggta tcccgattaa ggaggtttcg ttatgaaatt      180

acccaggtca agtctagtat agtgcgttct gatcgtgtgc ttgaccttgc tgatcttcac      240

ctatctgacg cgtaagtccc tgtgtgaaat tcgttaccgc gacggccacc gtgaggtcgc      300

tgcgtttatg gcatacgaga gcggtaaata acagaaaaaa gcccgcacct gacagtgcgg      360

gctttttttt tcga                                                        374


<210>  9
<211>  4780
<212>  DNA
<213>  Artificial

<220>
<223>  pEVOL MJWTyRS-CUA plasmid

<400>  9
atgccatagc atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac       60

tctctactgt ttctccatac ccgttttttt gggctaacag gaggaattag atctatggat      120

gaattcgaaa tgatcaagcg caatacgtcg gagatcatct ctgaagaaga attacgtgag      180

gttttaaaaa aagacgaaaa atcggcctat attggctttg aaccatcagg aaaaatccac      240

cttggtcact accttcagat taagaaaatg attgacctgc aaaatgcagg tttcgacatt      300

attatcctgc tggccgacct gcatgcgtat cttaatcaaa aaggcgaatt ggatgagatc      360

cgcaagatcg gagattataa caagaaagta ttcgaggcta tgggtctgaa agcaaagtat      420

gtgtatgggt cggagttcca gcttgacaag gactataccc ttaacgtcta ccgtcttgct      480

ttgaaaacca ccttaaagcg tgcgcgtcgc agcatggagc tgatcgctcg tgaggacgag      540

aacccgaaag tcgctgaggt aatctatccc attatgcaag tcaatgacat tcactacctt      600

ggggtggatg tcgcagtggg cgggatggaa caacgtaaga ttcacatgct ggcacgcgaa      660

ctgcttccca agaaggtcgt gtgcatccac aatccggtat tgactggcct tgacggggag      720

ggaaaaatgt ccagctcgaa gggaaatttt atcgcagttg atgactcccc tgaggagatt      780

cgtgctaaaa tcaagaaggc gtattgtcca gcaggcgtag ttgagggcaa tcccatcatg      840

gaaattgcga aatattttct tgaatacccc cttacgatta agcgtccaga gaagtttggt      900

ggcgatctga cggtcaactc ttacgaggag ctggaatcct tatttaagaa taaggaactg      960

cacccaatgg accttaaaaa cgcggttgcg gaagagttga ttaaaatctt agagcccatc     1020

cgtaagcgtc tttaactgca gtttcaaacg ctaaattgcc tgatgcgcta cgcttatcag     1080

gcctacatga tctctgcaat atattgagtt tgcgtgcttt tgtaggccgg ataaggcgtt     1140

cacgccgcat ccggcaagaa acagcaaaca atccaaaacg ccgcgttcag cggcgttttt     1200

tctgcttttc ttcgcgaatt aattccgctt cgcaacatgt gagcaccggt ttattgacta     1260

ccggaagcag tgtgaccgtg tgcttctcaa atgcctgagg ccagtttgct caggctctcc     1320

ccgtggaggt aataattgac gatatgatca gtgcacggct aactaagcgg cctgctgact     1380

ttctcgccga tcaaaaggca ttttgctatt aagggattga cgagggcgta tctgcgcagt     1440

aagatgcgcc ccgcattccg gcggtagttc agcagggcag aacggcggac tctaaatccg     1500

catggcaggg gttcaaatcc cctccgccgg accaaattcg aaaagcctgc tcaacgagca     1560

ggcttttttg catgctcgag cagctcaggg tcgaatttgc tttcgaattt ctgccattca     1620

tccgcttatt atcacttatt caggcgtagc accaggcgtt taagggcacc aataactgcc     1680

ttaaaaaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat     1740

tctgccgaca tggaagccat cacagacggc atgatgaacc tgaatcgcca gcggcatcag     1800

caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc     1860

catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa     1920

aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac     1980

atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga     2040

tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat     2100

caccagctca ccgtctttca ttgccatacg gaattccgga tgagcattca tcaggcgggc     2160

aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa     2220

ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc     2280

ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt     2340

tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg     2400

tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca     2460

ttttcgccaa aagttggccc agggcttccc ggtatcaaca gggacaccag gatttattta     2520

ttctgcgaag tgatcttccg tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc     2580

tgccaactta ctgatttagt gtatgatggt gtttttgagg tgctccagtg gcttctgttt     2640

ctatcagctg tccctcctgt tcagctactg acggggtggt gcgtaacggc aaaagcaccg     2700

ccggacatca gcgctagcgg agtgtatact ggcttactat gttggcactg atgagggtgt     2760

cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca gcagaatatg     2820

tgatacagga tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg     2880

cggcgagcgg aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata     2940

cttaacaggg aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg     3000

acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa     3060

gataccaggc gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt     3120

ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt     3180

tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac     3240

cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca     3300

ccactggcag cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg     3360

ttaaggctaa actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg     3420

gttcaaagag ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc     3480

gttttcagag caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa     3540

tcagataaaa tatttctaga tttcagtgca atttatctct tcaaatgtag cacctgaagt     3600

cagccccata cgatataagt tgtaattctc atgtttgaca gcttatcatc gataagcttg     3660

gtacccaatt atgacaactt gacggctaca tcattcactt tttcttcaca accggcacgg     3720

aactcgctcg ggctggcccc ggtgcatttt ttaaataccc gcgagaaata gagttgatcg     3780

tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtggtgct caaaagcagc     3840

ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc taactgctgg     3900

cggaaaagat gtgacagacg cgacggcgac aagcaaacat gctgtgcgac gctggcgata     3960

tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg tacccgatta     4020

tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa caattgctca     4080

agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc gttaatgatt     4140

tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta     4200

ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa     4260

acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg aatctctcct     4320

ggcgggaaca gcaaaatatc actcggtcgg caaacaaatt ctcgtccctg atttttcacc     4380

accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag cggtcggtcg     4440

ataaaaaaat cgagataacc gttggcctca atcggcgtta aacccgccac cagatgggca     4500

ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatact tttcatactc     4560

ccgccattca gagaagaaac caattgtcca tattgcatca gacattgccg tcactgcgtc     4620

ttttactggc tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag cattctgtaa     4680

caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa tcacggcaga     4740

aaagtccaca ttgattattt gcacggcgtc acactttgct                           4780


<210>  10
<211>  3198
<212>  DNA
<213>  Artificial

<220>
<223>  pUC57-Kan-Kid plasmid

<400>  10
tgcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat       60

catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc      120

atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg      180

ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg      240

ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg      300

ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca      360

agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa      420

cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg      480

cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc      540

gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg      600

attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac      660

ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta      720

tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc      780

gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga      840

aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt      900

tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacat tattcagatt      960

gggcttgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat     1020

aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta     1080

gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa     1140

acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt     1200

tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag     1260

ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta     1320

atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca     1380

agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag     1440

cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa     1500

agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga     1560

acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc     1620

gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc     1680

ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt     1740

gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt     1800

gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag     1860

gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa     1920

tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat     1980

gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg     2040

ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac     2100

accaagcttg catgcaggcc tctgcagtcg acgggcccgg gatccgatca cagctaacac     2160

cacgtcgtcc ctatctgctg ccctaggtct atgagtggtt gctggataac tttacgggca     2220

tgcataaggc tcgtatgata tattcaggga gaccacaacg gtttccctct acaaataatt     2280

ttgtttaact ttataaacac atctaaggag gtaaagacct gtatgttaaa atagcaacta     2340

aagaacgaaa atggctggat gcatcgtcgc ttggtgcgtc gtaaaagcga tatggaacgt     2400

ggcgaaattt agctggtctc cctggacccg accgcgggcc acgagcagca gggcacccgc     2460

ccagttttga tcgtgacccc ggctgcgttc aaccgtgtta cccgtctgcc ggtcgtggtg     2520

ccggtgacga gcggtggtaa tttcgccaga actgcgggtt ttgcagtttc tctggacggc     2580

gttggtattc gtactacggg tgttgtacgc tgcgatcaac cgcgtaccat cgacatgaaa     2640

gctcgtggtg gcaagcgcct tgagcgcgtg ccggaaacca ttatgaacga ggttctgggt     2700

cgtttaagca ccatcctgac ctaacagaaa aaagcccgca cctgacagtg cgggcttttt     2760

ttttcgaatc tagatgtatt cgcgaggtac cgagctcgaa ttctctggcc gtcgttttac     2820

aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc     2880

ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc     2940

gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta     3000

tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc     3060

agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat     3120

ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt     3180

catcaccgaa acgcgcga                                                   3198


<210>  11
<211>  3132
<212>  DNA
<213>  Artificial

<220>
<223>  pUC57-Kan-mazF plasmid

<400>  11
tgcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat       60

catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc      120

atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg      180

ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg      240

ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg      300

ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca      360

agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa      420

cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg      480

cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc      540

gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg      600

attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac      660

ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta      720

tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc      780

gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga      840

aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt      900

tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacat tattcagatt      960

gggcttgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat     1020

aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta     1080

gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa     1140

acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt     1200

tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag     1260

ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta     1320

atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca     1380

agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag     1440

cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa     1500

agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga     1560

acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc     1620

gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc     1680

ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt     1740

gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt     1800

gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag     1860

gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa     1920

tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat     1980

gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg     2040

ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac     2100

accaagcttg catgcaggcc tctgcagtcg acgggcccgg gatccgatca cagctaacac     2160

cacgtcgtcc ctatctgctg ccctaggtct atgagtggtt gctggataac tttacgggca     2220

tgcataaggc tcgtatgata tattcaggga gaccacaacg gtttccctct acaaataatt     2280

ttgtttaact ttactacgac gttactttaa ggaggatttt taatggtttc aaggtatgta     2340

cccgatatgg gagacctgat ctgggttgac ttcgacccga ccaagggcag cgagcaggcg     2400

ggtcaccgtc cagcagttgt gttgtccccg ttcatgtaga ataacaagac tggtatgtgc     2460

ctgtgcgttc cgtgtaccac gcagagcaaa ggctagccgt ttgaagtggt gttgtctggt     2520

caagagcgcg atggtgtagc cctggcggat caggttaaaa gcattgcatg gcgtgcgcgt     2580

ggcgctacca agaaaggaac cgtcgctccg gaagagctgc aactgatcaa agcgaagatc     2640

aacgtgttaa ttggctaaca gaaaaaagcc cgcacctgac agtgcgggct ttttttttcg     2700

aatctagatg tattcgcgag gtaccgagct cgaattctct ggccgtcgtt ttacaacgtc     2760

gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg     2820

ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc     2880

tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac     2940

accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc     3000

gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt     3060

acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac     3120

cgaaacgcgc ga                                                         3132


<210>  12
<211>  2922
<212>  DNA
<213>  Artificial

<220>
<223>  pUC57-Kan-timP plasmid

<400>  12
tgcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat       60

catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc      120

atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg      180

ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg      240

ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg      300

ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca      360

agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa      420

cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg      480

cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc      540

gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg      600

attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac      660

ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta      720

tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc      780

gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga      840

aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt      900

tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacat tattcagatt      960

gggcttgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat     1020

aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta     1080

gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa     1140

acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt     1200

tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag     1260

ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta     1320

atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca     1380

agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag     1440

cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa     1500

agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga     1560

acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc     1620

gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc     1680

ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt     1740

gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt     1800

gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag     1860

gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa     1920

tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat     1980

gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg     2040

ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac     2100

accaagcttg catgcaggcc tctgcagtcg acgggcccgg gatccgatca cagctaacac     2160

cacgtcgtcc ctatctgctg ccctaggtct atgagtggtt gctggataac tttacgggca     2220

tgcataaggc tcgtatgata tattcaggga gaccacaacg gtttccctct acaaataatt     2280

ttgtttaact ttataaaata atcaaaataa ggaggagacg tcatgaaaat aaggtgcttt     2340

tgtattgtac taatcgtgag cggtgcgctg tagaccgagg ttaataacaa tcgtagctag     2400

tccggcgaca acctgttggt tgtgaacaac ctgcagtcta gcaagtaaca gaaaaaagcc     2460

cgcacctgac agtgcgggct ttttttttcg aatctagatg tattcgcgag gtaccgagct     2520

cgaattctct ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac     2580

ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca     2640

ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt     2700

ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct     2760

gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct     2820

gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct     2880

gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc ga                        2922


<210>  13
<211>  5042
<212>  DNA
<213>  Artificial

<220>
<223>  p15A-hGHR wt-RC55 plasmid

<400>  13
ccaattattg aaggccgcta acgcggcctt tttttgtttc tggtctccct ctatcagctg       60

tccctcctgt tcagctactg acggggtggt gcgtaacggc aaaagcaccg ccggacatca      120

gcgctagcgg agtgtatact ggcttactat gttggcactg atgagggtgt cagtgaagtg      180

cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca gcagaatatg tgatacagga      240

tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg      300

aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg      360

aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca      420

cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc      480

gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt      540

cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg      600

cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct      660

tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag      720

cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa      780

actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag      840

ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag      900

caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa tcagataaaa      960

tatttctaga tttcagtgca atttatctct tcaaatgtag cacctgaagt cagccccata     1020

cgatataagt tgtaccaccg tcaaaaaaaa cggcgctttt tagcgccgtt tttatttttc     1080

aaccttttag gacagcttga cggctacatc attcactttt tcttcacaac cggcacggaa     1140

ctcggacgga gaggccccgg tgcatttttt aaatacccga gagaaataga gttgatcgtc     1200

aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg gtggtggaca gaagcagctt     1260

cgcctgagag atacgttggt cctcgcgcca ggacaggaca gaaatcccga gctgctggcg     1320

gaaaagatga gacagacgag acggagagag gcaaacatgc tgtgcgacag aggcgatatc     1380

aaaattagag tctgccaggt gatcagagat gtactgacaa gcctcgcgta cccgattatc     1440

catcggtgga tggagggact cgttaatcgc ttccatgcgc cgcagaagta gttgctcaag     1500

cagatttatc gccagcagct cagaatagcg cccttcccct tgcccggcgt taatgatttg     1560

cccaaacagg tcggagaaat gcggctggtg cgcttcatcc gggcgaaaga accccgtatt     1620

ggcaaatata gacggccagt taagccattc atgccagtag gcgcgcggac gaaagtaaac     1680

ccactggtga taccattcgc gagcctccgg atgacgaccg tagtgatgaa tctctcctgg     1740

cgggaacaga agaatatcac ccggtcggca aacaaattct cgtccctgat ttttcaccac     1800

cccctgaccg cgaatggtga gattgagaat ataacctttc attcccagcg gtcggtcgat     1860

aaaaaaatcg agataaccgt tggcctcaat cggcgtaaga cccgccacca gatgggcatt     1920

aaaagagtat cccggcagca ggggatcatt ttgcgcttca gccatacttt tcatactccc     1980

gccattcaga gaagaaacca attgtccata ttgcatcaga cattgccgtc actgcgtctt     2040

ttactggctc ttctcgctaa ccaaaccggt aaccccgctt attaaaagca ttctgtaaca     2100

aagcgggacc aaagccatga caaaaacgcg taacaaaagt gtctataatc acggcagaaa     2160

agtccacatt gattatttgc acggcgtcac actttgctat gccatagcat ttttatccat     2220

aagattagcg gatcctacct gacgcttttt atcgcaactc tctactgttt ctccataccc     2280

atggatctct ggcagctgct gttgaccttg gcactggcag gatcaagtga tgctttttct     2340

ggaagtgagg ccacagcagc tatccttagc agagcaccct ggagtctgca aagtgttaat     2400

ccaggcctaa agacaaattc ttctaaggag cctaaattca ccaagtgccg ttcacctgag     2460

cgagagactt tttcatgcca ctggacagat gaggttcatc atggtacaaa gaacctagga     2520

cccatacagc tgttctatac cagaaggaac actcaagaat ggactcaaga atggaaagaa     2580

tgccctgatt atgtttctgc tggggaaaac agctgttact ttaattcatc gtttacctcc     2640

atctggatac cttattgtat caagctaact agcaatggtg gtacagtgga tgaaaagtgt     2700

ttctctgttg atgaaatagt gcaaccagat ccacccattg ccctcaactg gactttactg     2760

aacgtcagtt taactgggat tcatgcagat atccaagtga gatgggaagc accacgcaat     2820

gcagatattc agaaaggatg gatggttctg gagtatgaac ttcaatacaa agaagtaaat     2880

gaaactaaat ggaaaatgat ggaccctata ttgacaacat cagttccagt gtactcattg     2940

aaagtggata aggaatatga agtgcgtgtg agatccaaac aacgaaactc tggaaattat     3000

ggcgagttca gtgaggtgct ctatgtaaca cttcctcaga tgagccaatt tacatgtgaa     3060

gaagatttct actttccatg gctcttaatt attatctttg gaatatttgg gctaacagtg     3120

atgctatttg tattcttatt ttctaaacag caaaggatta aaatgctgat tctgccccca     3180

gttccagttc caaagattaa aggaatcgat ccagatctcc tcaaggaagg aaaattagag     3240

gaggtgaaca caatcttagc cattcatgat agctataaac ccgaattcca cagtgatgac     3300

tcttgggttg aatttattga gctagatatt gatgagccag atgaaaagac tgaggaatca     3360

gacacagaca gacttctaag cagtgaccat gagaaatcac atagtaacct aggggtgaag     3420

gatggcgact ctggacgtac cagctgttgt gaacctgaca ttctggagac tgatttcaat     3480

gccaatgaca tacatgaggg tacctcagag gttgctcagc cacagaggtt aaaaggggaa     3540

gcagatctct tatgccttga ccagaagaat caaaataact caccttatca tgatgcttgc     3600

cctgctactc agcagcccag tgttatccaa gcagagaaaa acaaaccaca accacttcct     3660

actgaaggag ctgagtcaac tcaccaagct gcccatattc agctaagcaa tccaagttca     3720

ctgtcaaaca tcgactttta tgcccaggtg agcgacatta caccagcagg tagtgtggtc     3780

ctttccccgg gccaaaagaa taaggcaggg atgtcccaat gtgacatgca cccggaaatg     3840

gtctcactct gccaagaaaa cttccttatg gacaatgcct acttctgtga ggcagatgcc     3900

aaaaagtgca tccctgtggc tcctcacatc aaggttgaat cacacataca gccaagctta     3960

aaccaagagg acatttacat caccacagaa agccttacca ctgctgctgg gaggcctggg     4020

acaggagaac atgttccagg ttctgagatg cctgtcccag actatacctc cattcatata     4080

gtacagtccc cacagggcct catactcaat gcgactgcct tgcccttgcc tgacaaagag     4140

tttctctcat catgtggcta tgtgagcaca gaccaactga acaaaatcat gccttagagt     4200

caaaagcctc cgaccggagg cttttgactc ctgttgatac cgggaagccc tgggccaact     4260

tttggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac cataatgaaa     4320

taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta     4380

aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag     4440

aacattttga ggcatttcag tccgttgctc aatgtaccta taaccagacc gttcagctgg     4500

atattacggc ctttctgaag accgtaaaga aaaataagca caagttttat ccggccttta     4560

ttcacattct tgcccgcctg atgaatgctc atccggaatt tcgtatggca atgaaagacg     4620

gtgagctggt gatatgggat tctgttcacc cttgttacac cgttttccat gagcaaactg     4680

aaacgttttc ctctctctgg tccgaatacc acgacgattt ccggcagttt ctacacatat     4740

attcccaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg     4800

agaatatgtt tttcgtctcc gccaatccct gggtgtcctt cacctccttt gatctcaacg     4860

tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag     4920

gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc     4980

atgtcggccg catgcttaat gaactgcaac agtactgcga tgagtggcag ggcggggcgt     5040

aa                                                                    5042


<210>  14
<211>  52
<212>  PRT
<213>  Artificial

<220>
<223>  Hok protein

<400>  14

Met Lys Leu Pro Arg Ser Ser Leu Val Trp Cys Val Leu Ile Val Cys 
1               5                   10                  15      


Leu Thr Leu Leu Ile Phe Thr Tyr Leu Thr Arg Lys Ser Leu Cys Glu 
            20                  25                  30          


Ile Arg Tyr Arg Asp Gly His Arg Glu Val Ala Ala Phe Met Ala Tyr 
        35                  40                  45              


Glu Ser Gly Lys 
    50          


<210>  15
<211>  133
<212>  PRT
<213>  Artificial

<220>
<223>  Kid protein

<400>  15

Met Leu Lys Tyr Gln Leu Lys Asn Glu Asn Gly Trp Met His Arg Arg 
1               5                   10                  15      


Leu Val Arg Arg Lys Ser Asp Met Glu Arg Gly Glu Ile Trp Leu Val 
            20                  25                  30          


Ser Leu Asp Pro Thr Ala Gly His Glu Gln Gln Gly Thr Arg Pro Val 
        35                  40                  45              


Leu Ile Val Thr Pro Ala Ala Phe Asn Arg Val Thr Arg Leu Pro Val 
    50                  55                  60                  


Val Val Pro Val Thr Ser Gly Gly Asn Phe Ala Arg Thr Ala Gly Phe 
65                  70                  75                  80  


Ala Val Ser Leu Asp Gly Val Gly Ile Arg Thr Thr Gly Val Val Arg 
                85                  90                  95      


Cys Asp Gln Pro Arg Thr Ile Asp Met Lys Ala Arg Gly Gly Lys Arg 
            100                 105                 110         


Leu Glu Arg Val Pro Glu Thr Ile Met Asn Glu Val Leu Gly Arg Leu 
        115                 120                 125             


Ser Thr Ile Leu Thr 
    130             


<210>  16
<211>  111
<212>  PRT
<213>  Artificial

<220>
<223>  mazF protein

<400>  16

Met Val Ser Arg Tyr Val Pro Asp Met Gly Asp Leu Ile Trp Val Asp 
1               5                   10                  15      


Phe Asp Pro Thr Lys Gly Ser Glu Gln Ala Gly His Arg Pro Ala Val 
            20                  25                  30          


Val Leu Ser Pro Phe Met Tyr Asn Asn Lys Thr Gly Met Cys Leu Cys 
        35                  40                  45              


Val Pro Cys Thr Thr Gln Ser Lys Gly Tyr Pro Phe Glu Val Val Leu 
    50                  55                  60                  


Ser Gly Gln Glu Arg Asp Gly Val Ala Leu Ala Asp Gln Val Lys Ser 
65                  70                  75                  80  


Ile Ala Trp Arg Ala Arg Gly Ala Thr Lys Lys Gly Thr Val Ala Pro 
                85                  90                  95      


Glu Glu Leu Gln Leu Ile Lys Ala Lys Ile Asn Val Leu Ile Gly 
            100                 105                 110     


<210>  17
<211>  41
<212>  PRT
<213>  Artificial

<220>
<223>  timP protein

<400>  17

Met Lys Ile Arg Cys Phe Cys Ile Val Leu Ile Val Ser Gly Ala Leu 
1               5                   10                  15      


Leu Thr Glu Val Asn Asn Asn Arg Ser Leu Ser Gly Asp Asn Leu Leu 
            20                  25                  30          


Val Val Asn Asn Leu Gln Ser Ser Lys 
        35                  40      


