
                                SEQUENCE LISTING

<110> Seattle Children's Hospital
      Jensen, Michael
      Wei, Jia

<120> ANTI-EGFR CHIMERIC ANTIGEN RECEPTORS

<130> SCRI.348WO

<150> 63122839            
<151> 2020-12-08  

<150> 63234090            
<151> 2021-08-17  

<160> 10

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 726
<212> DNA
<213> Artificial Sequence

<220> 
<223> Codon-optimized version of the sequence occuring
      in Homo Sapiens

<400> 1
gacgtccagc tgcaagagtc tggccctagc ctggtcaagc ctagccagag cctgagcctg 60
acatgtaccg tgaccggcta cagcatcacc agcgacttcg cctggaactg gatcagacag 120
ttccccggca acaagctgga atggatgggc tacatcagct acagcggcaa cacccggtac 180
aaccccagcc tgaagtcccg gatctccatc accagagaca ccagcaagaa ccagttcttc 240
ctgcagctga acagcgtgac catcgaggac accgccacct actactgtgt gacagccggc 300
agaggcttcc cttattgggg acagggaacc ctggtcacag tgtctgccgg aagcacatct 360
ggctctggca aacctggatc tggcgagggc tctaccaagg gcgacatcct gatgacacag 420
agccccagca gcatgtctgt gtccctgggc gataccgtgt ccatcacctg tcacagcagc 480
caggacatca acagcaacat cggctggctg cagcagaggc ctggcaagtc ttttaagggc 540
ctgatctacc acggcaccaa cctggatgat gaggtgccca gcagattttc cggctctgga 600
agcggagccg actactccct gacaatcagc agcctggaaa gcgaggactt cgccgattac 660
tactgcgtgc agtacgccca gtttccttgg acctttggcg gaggcacaaa gctggaaatc 720
aagcgc                                                            726

<210> 2
<211> 544
<212> DNA
<213> Homo sapiens

<400> 2
ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg 60
agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa 120
actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt 180
atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac 240
agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc 300
gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg 360
cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc 420
cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac 480
tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc 540
ctac                                                              544

<210> 3
<211> 66
<212> DNA
<213> Artificial Sequence

<220> 
<223> Codon-optimized version of the sequence occuring
      in Porcine teschovirus-1 2A

<400> 3
ggaagcggcg ccacaaattt cagcctgctg aaacaggccg gcgacgtgga agagaaccct 60
ggacct                                                            66

<210> 4
<211> 63
<212> DNA
<213> Artificial Sequence

<220> 
<223> Codon-optimized version of the sequence occuring
      in Thosea asigna virus 2A

<400> 4
ggcggaggcg aaggcagagg ttctctgctt acatgcggag atgtggaaga aaatcccggg 60
cct                                                               63

<210> 5
<211> 561
<212> DNA
<213> Artificial Sequence

<220> 
<223> Codon-optimized version of the sequence occuring
      in Homo sapiens

<400> 5
atggtcggaa gcctgaactg catcgtggcc gtgtctcaga acatgggcat cggcaagaac 60
ggcgacttcc cttggcctcc tctgagaaac gagagccggt acttccagcg gatgaccacc 120
acaagcagcg tggaaggcaa gcagaacctg gtcatcatgg gcaagaaaac ctggttcagc 180
atccctgaga agaacagacc cctgaagggc agaatcaacc tggtgctgag cagagagctg 240
aaagagcctc ctcagggcgc ccactttctg agcagatctc tggacgatgc cctgaagctg 300
accgagcaac ctgagctggc caacaaggtg gacatggtct ggatcgttgg cggcagcagc 360
gtgtacaaag aagccatgaa tcaccccggc cacctgaaac tgttcgtgac cagaatcatg 420
caggacttcg agagcgacac attcttccca gagatcgacc tggaaaagta caaactgctg 480
cctgagtacc ccggcgtgct gagcgacgtg caagaagaga aaggcatcaa gtacaagttc 540
gaggtgtacg agaagaacga c                                           561

<210> 6
<211> 1005
<212> DNA
<213> Artificial Sequence

<220> 
<223> Codon-optimized version of the sequence occuring
      in Homo sapiens

<400> 6
cggaaagtgt gcaacggcat cggaatcggc gagttcaagg acagcctgag catcaacgcc 60
accaacatca agcacttcaa gaactgcacc agcatcagcg gcgacctgca cattctgcct 120
gtggccttta gaggcgacag cttcacccac acacctccac tggatcccca agagctggat 180
atcctgaaaa ccgtgaaaga gatcaccgga tttctgttga tccaggcttg gcccgagaac 240
cggacagatc tgcacgcctt cgagaacctc gagatcatca gaggccggac caagcagcac 300
ggccagtttt ctctggccgt ggtgtccctg aatatcacct ctctgggcct gcgcagcctg 360
aaagaaatct ccgatggcga cgtgatcatc agcggaaaca agaacctgtg ctacgccaac 420
accatcaact ggaagaagct gttcggcacc tccggccaga aaacaaagat catctccaac 480
cggggcgaga actcctgcaa ggctacaggc caagtgtgcc acgctctgtg tagccctgaa 540
ggctgttggg gacccgagcc tagagattgc gtgtcctgca gaaacgtgtc ccggggcaga 600
gaatgcgtgg acaagtgcaa tctgctcgag ggcgagccac gcgagttcgt ggaaaacagc 660
gagtgcatcc agtgtcaccc cgagtgcctg cctcaggcca tgaacatcac atgcaccgga 720
agaggccccg acaactgtat ccagtgcgcc cactatatcg acggccctca ctgcgtgaaa 780
acctgtcctg ctggcgtgat gggagagaac aacaccctcg tgtggaagta cgccgatgcc 840
ggacatgtgt gccacctgtg tcaccctaat tgcacctacg gctgtacagg cccaggactg 900
gaaggctgcc ctacaaacgg acctaagatc cccagcattg ccaccggcat ggttggagcc 960
ctgctgcttc tgctggtggt ggcccttgga atcggactgt ttatg                 1005

<210> 7
<211> 462
<212> DNA
<213> Artificial Sequence

<220> 
<223> Codon-optimized version of the sequence occuring
      in Homo sapiens

<400> 7
aagcggggca gaaagaagct gctgtacatc ttcaagcagc ccttcatgcg gcccgtgcag 60
accacacaag aggaagatgg ctgctcctgc agattccccg aggaagaaga aggcggctgc 120
gagctgagag tgaagttcag cagatccgcc gacgctcctg cctatcagca gggacagaac 180
cagctgtaca acgagctgaa cctggggaga agagaagagt acgacgtgct ggacaagcgg 240
agaggcagag atcctgagat gggcggcaag cccagacgga agaatcctca agagggcctg 300
tataatgagc tgcagaaaga caagatggcc gaggcctaca gcgagatcgg aatgaagggc 360
gagcgcagaa gaggcaaggg acacgatgga ctgtaccagg gactgagcac cgccacaaag 420
gacacctatg acgccctgca catgcaggcc cttccaccta ga                    462

<210> 8
<211> 84
<212> DNA
<213> Artificial Sequence

<220> 
<223> Codon-optimized version of the sequence occuring
      in Homo sapiens

<400> 8
atgttctggg tgctcgtggt tgttggcgga gtgctggcct gttatagcct gcttgtgacc 60
gtggccttca tcatcttttg ggtc                                        84

<210> 9
<211> 36
<212> DNA
<213> Artificial Sequence

<220> 
<223> Codon-optimized version of the sequence occuring
      in Homo sapiens

<400> 9
gagtctaagt acggccctcc ttgtcctcca tgtcct                           36

<210> 10
<211> 3698
<212> DNA
<213> Artificial Sequence

<220> 
<223> Sequence comprising the EFa1/HTLV promoter, as
      well as the sequence encoding a polypeptide
      comprising codon-optimized human EGFR806scFV,
      IgG4, CD28tm, 41BB, CD3?, P2A, DHFRdm, T2A, and
      EGFRt

<400> 10
ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg 60
agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa 120
actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt 180
atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac 240
agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc 300
gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg 360
cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc 420
cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac 480
tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc 540
ctacggctag cgccgccacc atgttgctgc tggttacatc tctgctgctg tgcgagctgc 600
cccatcctgc ctttctgctg atccctgacg tccagctgca agagtctggc cctagcctgg 660
tcaagcctag ccagagcctg agcctgacat gtaccgtgac cggctacagc atcaccagcg 720
acttcgcctg gaactggatc agacagttcc ccggcaacaa gctggaatgg atgggctaca 780
tcagctacag cggcaacacc cggtacaacc ccagcctgaa gtcccggatc tccatcacca 840
gagacaccag caagaaccag ttcttcctgc agctgaacag cgtgaccatc gaggacaccg 900
ccacctacta ctgtgtgaca gccggcagag gcttccctta ttggggacag ggaaccctgg 960
tcacagtgtc tgccggaagc acatctggct ctggcaaacc tggatctggc gagggctcta 1020
ccaagggcga catcctgatg acacagagcc ccagcagcat gtctgtgtcc ctgggcgata 1080
ccgtgtccat cacctgtcac agcagccagg acatcaacag caacatcggc tggctgcagc 1140
agaggcctgg caagtctttt aagggcctga tctaccacgg caccaacctg gatgatgagg 1200
tgcccagcag attttccggc tctggaagcg gagccgacta ctccctgaca atcagcagcc 1260
tggaaagcga ggacttcgcc gattactact gcgtgcagta cgcccagttt ccttggacct 1320
ttggcggagg cacaaagctg gaaatcaagc gcgagtctaa gtacggccct ccttgtcctc 1380
catgtcctat gttctgggtg ctcgtggttg ttggcggagt gctggcctgt tatagcctgc 1440
ttgtgaccgt ggccttcatc atcttttggg tcaagcgggg cagaaagaag ctgctgtaca 1500
tcttcaagca gcccttcatg cggcccgtgc agaccacaca agaggaagat ggctgctcct 1560
gcagattccc cgaggaagaa gaaggcggct gcgagctgag agtgaagttc agcagatccg 1620
ccgacgctcc tgcctatcag cagggacaga accagctgta caacgagctg aacctgggga 1680
gaagagaaga gtacgacgtg ctggacaagc ggagaggcag agatcctgag atgggcggca 1740
agcccagacg gaagaatcct caagagggcc tgtataatga gctgcagaaa gacaagatgg 1800
ccgaggccta cagcgagatc ggaatgaagg gcgagcgcag aagaggcaag ggacacgatg 1860
gactgtacca gggactgagc accgccacaa aggacaccta tgacgccctg cacatgcagg 1920
cccttccacc tagaggaagc ggcgccacaa atttcagcct gctgaaacag gccggcgacg 1980
tggaagagaa ccctggacct atggtcggaa gcctgaactg catcgtggcc gtgtctcaga 2040
acatgggcat cggcaagaac ggcgacttcc cttggcctcc tctgagaaac gagagccggt 2100
acttccagcg gatgaccacc acaagcagcg tggaaggcaa gcagaacctg gtcatcatgg 2160
gcaagaaaac ctggttcagc atccctgaga agaacagacc cctgaagggc agaatcaacc 2220
tggtgctgag cagagagctg aaagagcctc ctcagggcgc ccactttctg agcagatctc 2280
tggacgatgc cctgaagctg accgagcaac ctgagctggc caacaaggtg gacatggtct 2340
ggatcgttgg cggcagcagc gtgtacaaag aagccatgaa tcaccccggc cacctgaaac 2400
tgttcgtgac cagaatcatg caggacttcg agagcgacac attcttccca gagatcgacc 2460
tggaaaagta caaactgctg cctgagtacc ccggcgtgct gagcgacgtg caagaagaga 2520
aaggcatcaa gtacaagttc gaggtgtacg agaagaacga cggcggaggc gaaggcagag 2580
gttctctgct tacatgcgga gatgtggaag aaaatcccgg gcctatgctg ctgctcgtga 2640
caagcctgct cctgtgtgaa ctccctcatc cagcttttct gctcattccc cggaaagtgt 2700
gcaacggcat cggaatcggc gagttcaagg acagcctgag catcaacgcc accaacatca 2760
agcacttcaa gaactgcacc agcatcagcg gcgacctgca cattctgcct gtggccttta 2820
gaggcgacag cttcacccac acacctccac tggatcccca agagctggat atcctgaaaa 2880
ccgtgaaaga gatcaccgga tttctgttga tccaggcttg gcccgagaac cggacagatc 2940
tgcacgcctt cgagaacctc gagatcatca gaggccggac caagcagcac ggccagtttt 3000
ctctggccgt ggtgtccctg aatatcacct ctctgggcct gcgcagcctg aaagaaatct 3060
ccgatggcga cgtgatcatc agcggaaaca agaacctgtg ctacgccaac accatcaact 3120
ggaagaagct gttcggcacc tccggccaga aaacaaagat catctccaac cggggcgaga 3180
actcctgcaa ggctacaggc caagtgtgcc acgctctgtg tagccctgaa ggctgttggg 3240
gacccgagcc tagagattgc gtgtcctgca gaaacgtgtc ccggggcaga gaatgcgtgg 3300
acaagtgcaa tctgctcgag ggcgagccac gcgagttcgt ggaaaacagc gagtgcatcc 3360
agtgtcaccc cgagtgcctg cctcaggcca tgaacatcac atgcaccgga agaggccccg 3420
acaactgtat ccagtgcgcc cactatatcg acggccctca ctgcgtgaaa acctgtcctg 3480
ctggcgtgat gggagagaac aacaccctcg tgtggaagta cgccgatgcc ggacatgtgt 3540
gccacctgtg tcaccctaat tgcacctacg gctgtacagg cccaggactg gaaggctgcc 3600
ctacaaacgg acctaagatc cccagcattg ccaccggcat ggttggagcc ctgctgcttc 3660
tgctggtggt ggcccttgga atcggactgt ttatgtag                         3698
