               Sequence listing

<110> JSC "BIOCAD"

<120> Immunocytokine for activating human IL-10Ra receptor and use thereof

<150> RU2020140807
<151> 2020-12-10

<160> 6

<170> BiSSAP 1.3.6

<210> 1
<211> 402
<212> PRT
<213> Artificial sequence


<220> 
<223> IL-10-Fc monomer

<400> 1
Glu Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      
Pro Glu Ala Ala Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          
Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              
Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  
Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  
Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      
Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         
Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             
Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr 
    130                 135                 140                 
Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 
Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     
Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         
Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             
Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 
Ser Leu Ser Leu Ser Pro Gly Gly Gly Ser Gly Gly Gly Pro Gly Ser 
225                 230                 235                 240 
Gly Gly Ser Pro Gly Gln Gly Thr Gln Ser Glu Asn Ser Cys Thr His 
                245                 250                 255     
Phe Pro Gly Asn Leu Pro Asn Met Leu Arg Asp Leu Arg Asp Ala Phe 
            260                 265                 270         
Ser Arg Val Lys Thr Phe Phe Gln Met Lys Asp Gln Leu Asp Asn Leu 
        275                 280                 285             
Leu Leu Lys Glu Ser Leu Leu Glu Asp Phe Lys Gly Tyr Leu Gly Cys 
    290                 295                 300                 
Gln Ala Leu Ser Glu Met Ile Gln Phe Tyr Leu Glu Glu Val Met Pro 
305                 310                 315                 320 
Gln Ala Glu Asn Gln Asp Pro Asp Ile Lys Ala His Val Asn Ser Leu 
                325                 330                 335     
Gly Glu Asn Leu Lys Thr Leu Arg Leu Arg Leu Arg Arg Cys His Arg 
            340                 345                 350         
Phe Leu Pro Cys Glu Asn Lys Ser Lys Ala Val Glu Gln Val Lys Asn 
        355                 360                 365             
Ala Phe Asn Lys Leu Gln Glu Lys Gly Ile Tyr Lys Ala Met Ser Glu 
    370                 375                 380                 
Phe Asp Ile Phe Ile Asn Tyr Ile Glu Ala Tyr Met Thr Met Lys Ile 
385                 390                 395                 400 
Arg Asn 
        

<210> 2
<211> 421
<212> PRT
<213> Artificial sequence


<220> 
<223> мономер IL-10-Fc с лидерным пептидом

<400> 2
Met Met Ser Phe Val Ser Leu Leu Leu Val Gly Ile Leu Phe His Ala 
1               5                   10                  15      
Thr Gln Ala Glu Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro 
            20                  25                  30          
Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe Leu Phe Pro 
        35                  40                  45              
Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr 
    50                  55                  60                  
Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn 
65                  70                  75                  80  
Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg 
                85                  90                  95      
Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val 
            100                 105                 110         
Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser 
        115                 120                 125             
Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys 
    130                 135                 140                 
Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu 
145                 150                 155                 160 
Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe 
                165                 170                 175     
Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 
            180                 185                 190         
Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 
        195                 200                 205             
Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly 
    210                 215                 220                 
Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr 
225                 230                 235                 240 
Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Gly Gly Ser Gly Gly Gly 
                245                 250                 255     
Pro Gly Ser Gly Gly Ser Pro Gly Gln Gly Thr Gln Ser Glu Asn Ser 
            260                 265                 270         
Cys Thr His Phe Pro Gly Asn Leu Pro Asn Met Leu Arg Asp Leu Arg 
        275                 280                 285             
Asp Ala Phe Ser Arg Val Lys Thr Phe Phe Gln Met Lys Asp Gln Leu 
    290                 295                 300                 
Asp Asn Leu Leu Leu Lys Glu Ser Leu Leu Glu Asp Phe Lys Gly Tyr 
305                 310                 315                 320 
Leu Gly Cys Gln Ala Leu Ser Glu Met Ile Gln Phe Tyr Leu Glu Glu 
                325                 330                 335     
Val Met Pro Gln Ala Glu Asn Gln Asp Pro Asp Ile Lys Ala His Val 
            340                 345                 350         
Asn Ser Leu Gly Glu Asn Leu Lys Thr Leu Arg Leu Arg Leu Arg Arg 
        355                 360                 365             
Cys His Arg Phe Leu Pro Cys Glu Asn Lys Ser Lys Ala Val Glu Gln 
    370                 375                 380                 
Val Lys Asn Ala Phe Asn Lys Leu Gln Glu Lys Gly Ile Tyr Lys Ala 
385                 390                 395                 400 
Met Ser Glu Phe Asp Ile Phe Ile Asn Tyr Ile Glu Ala Tyr Met Thr 
                405                 410                 415     
Met Lys Ile Arg Asn 
            420     

<210> 3
<211> 1206
<212> DNA
<213> Artificial sequence


<220> 
<223> DNA IL-10-Fc

<400> 3
gagcccaaat ctagtgacaa aactcacaca tgcccaccgt gcccagcacc tgaagccgca      60

gggggaccgt cagtcttcct cttcccccca aaacccaagg acaccctcat gatctcccgg     120

acccctgagg tcacatgcgt ggtggtggac gtgagccacg aagaccctga ggtcaagttc     180

aactggtacg tggacggcgt ggaggtgcat aatgccaaga caaagccgcg ggaggagcag     240

tacaacagca cgtaccgtgt ggtcagcgtc ctcaccgtcc tgcaccagga ctggctgaat     300

ggcaaggagt acaagtgcaa ggtctccaac aaagccctcc cagcccccat cgagaaaacc     360

atctccaaag ccaaagggca gccccgagaa ccacaggtgt acaccctgcc cccatcccgg     420

gaggagatga ccaagaacca ggtcagcctg acctgcctgg tcaaaggctt ctatcccagc     480

gacatcgccg tggagtggga gagcaatggg cagccggaga acaactacaa gaccacgcct     540

cccgtgctgg actccgacgg ctccttcttc ctctatagca agctcaccgt ggacaagagc     600

aggtggcagc aggggaacgt cttctcatgc tccgtgatgc atgaggctct gcacaaccac     660

tacacgcaga aaagcctctc cctgtccccg ggtggagggt caggaggtgg tccaggatcg     720

ggtggctctc ctggacaggg aacccagtcc gagaactcct gcacccactt ccccggcaac     780

ctgcccaaca tgctgcggga cctgagagat gccttctcca gagtgaaaac attcttccag     840

atgaaggacc agctggacaa cctgctgctg aaagagtccc tgctggaaga tttcaagggc     900

tacctgggct gccaggccct gtccgagatg atccagttct acctggaaga agtgatgcct     960

caggccgaga accaggaccc cgacatcaag gcccacgtga actccctggg cgagaacctg    1020

aaaaccctgc ggctgcggct gagaagatgc caccggtttc tgccctgcga gaacaagtcc    1080

aaggccgtgg aacaagtgaa gaacgccttc aacaagctgc aggaaaaggg catctacaag    1140

gccatgagcg agttcgacat cttcatcaac tacatcgagg cctacatgac catgaagatc    1200

cggaac                                                               1206


<210> 4
<211> 1206
<212> DNA
<213> Artificial sequence


<220> 
<223> DNA IL-10-Fc

<400> 4
gagcccaaat ctagtgacaa aactcacaca tgcccaccgt gcccagcacc tgaagccgca      60

gggggaccgt cagtcttcct cttcccccca aaacccaagg acaccctcat gatctcccgg     120

acccctgagg tcacatgcgt ggtggtggac gtgagccacg aagaccctga ggtcaagttc     180

aactggtacg tggacggcgt ggaggtgcat aatgccaaga caaagccgcg ggaggagcag     240

tacaacagca cgtaccgtgt ggtcagcgtc ctcaccgtcc tgcaccagga ctggctgaat     300

ggcaaggagt acaagtgcaa ggtctccaac aaagccctcc cagcccccat cgagaaaacc     360

atctccaaag ccaaagggca gccccgagaa ccacaggtgt acaccctgcc cccatcccgg     420

gaggagatga ccaagaacca ggtcagcctg acctgcctgg tcaaaggctt ctatcccagc     480

gacatcgccg tggagtggga gagcaatggg cagccggaga acaactacaa gaccacgcct     540

cccgtgctgg actccgacgg ctccttcttc ctctatagca agctcaccgt ggacaagagc     600

aggtggcagc aggggaacgt cttctcatgc tccgtgatgc atgaggctct gcacaaccac     660

tacacgcaga aaagcctctc cctgtccccg ggtggagggt caggaggtgg tccaggatcg     720

ggtggctcgc ccggtcaagg aactcagtct gagaactcat gtacacactt tccagggaat     780

ctgcccaaca tgcttaggga tctccgggac gctttctcta gggtcaagac cttcttccag     840

atgaaagacc aactggacaa tctgctgtta aaggaaagtt tgctcgaaga ttttaagggc     900

tatctcgggt gccaggccct ttccgaaatg attcagtttt acctggagga agtaatgcct     960

caagctgaaa accaggaccc cgatatcaag gcgcatgtga attcattggg cgagaacctg    1020

aaaaccctcc gactcagact ccggcgttgc cacaggttcc tgccatgcga gaacaaatca    1080

aaggccgtcg agcaggtgaa gaacgcattc aataagctgc aggagaaagg aatctacaaa    1140

gctatgtcgg agttcgacat cttcatcaac tatatcgagg cttacatgac tatgaaaatc    1200

cgtaat                                                               1206


<210> 5
<211> 1263
<212> DNA
<213> Artificial sequence


<220> 
<223> DNA IL-10-Fc with a leader peptide

<400> 5
atgatgtcct ttgtctctct gctcctggtt ggcatcctgt tccatgccac ccaggccgag      60

cccaaatcta gtgacaaaac tcacacatgc ccaccgtgcc cagcacctga agccgcaggg     120

ggaccgtcag tcttcctctt ccccccaaaa cccaaggaca ccctcatgat ctcccggacc     180

cctgaggtca catgcgtggt ggtggacgtg agccacgaag accctgaggt caagttcaac     240

tggtacgtgg acggcgtgga ggtgcataat gccaagacaa agccgcggga ggagcagtac     300

aacagcacgt accgtgtggt cagcgtcctc accgtcctgc accaggactg gctgaatggc     360

aaggagtaca agtgcaaggt ctccaacaaa gccctcccag cccccatcga gaaaaccatc     420

tccaaagcca aagggcagcc ccgagaacca caggtgtaca ccctgccccc atcccgggag     480

gagatgacca agaaccaggt cagcctgacc tgcctggtca aaggcttcta tcccagcgac     540

atcgccgtgg agtgggagag caatgggcag ccggagaaca actacaagac cacgcctccc     600

gtgctggact ccgacggctc cttcttcctc tatagcaagc tcaccgtgga caagagcagg     660

tggcagcagg ggaacgtctt ctcatgctcc gtgatgcatg aggctctgca caaccactac     720

acgcagaaaa gcctctccct gtccccgggt ggagggtcag gaggtggtcc aggatcgggt     780

ggctctcctg gacagggaac ccagtccgag aactcctgca cccacttccc cggcaacctg     840

cccaacatgc tgcgggacct gagagatgcc ttctccagag tgaaaacatt cttccagatg     900

aaggaccagc tggacaacct gctgctgaaa gagtccctgc tggaagattt caagggctac     960

ctgggctgcc aggccctgtc cgagatgatc cagttctacc tggaagaagt gatgcctcag    1020

gccgagaacc aggaccccga catcaaggcc cacgtgaact ccctgggcga gaacctgaaa    1080

accctgcggc tgcggctgag aagatgccac cggtttctgc cctgcgagaa caagtccaag    1140

gccgtggaac aagtgaagaa cgccttcaac aagctgcagg aaaagggcat ctacaaggcc    1200

atgagcgagt tcgacatctt catcaactac atcgaggcct acatgaccat gaagatccgg    1260

aac                                                                  1263


<210> 6
<211> 1263
<212> DNA
<213> Artificial sequence


<220> 
<223> DNA IL-10-Fc with a leader peptide

<400> 6
atgatgtcct ttgtctctct gctcctggtt ggcatcctgt tccatgccac ccaggccgag      60

cccaaatcta gtgacaaaac tcacacatgc ccaccgtgcc cagcacctga agccgcaggg     120

ggaccgtcag tcttcctctt ccccccaaaa cccaaggaca ccctcatgat ctcccggacc     180

cctgaggtca catgcgtggt ggtggacgtg agccacgaag accctgaggt caagttcaac     240

tggtacgtgg acggcgtgga ggtgcataat gccaagacaa agccgcggga ggagcagtac     300

aacagcacgt accgtgtggt cagcgtcctc accgtcctgc accaggactg gctgaatggc     360

aaggagtaca agtgcaaggt ctccaacaaa gccctcccag cccccatcga gaaaaccatc     420

tccaaagcca aagggcagcc ccgagaacca caggtgtaca ccctgccccc atcccgggag     480

gagatgacca agaaccaggt cagcctgacc tgcctggtca aaggcttcta tcccagcgac     540

atcgccgtgg agtgggagag caatgggcag ccggagaaca actacaagac cacgcctccc     600

gtgctggact ccgacggctc cttcttcctc tatagcaagc tcaccgtgga caagagcagg     660

tggcagcagg ggaacgtctt ctcatgctcc gtgatgcatg aggctctgca caaccactac     720

acgcagaaaa gcctctccct gtccccgggt ggagggtcag gaggtggtcc aggatcgggt     780

ggctcgcccg gtcaaggaac tcagtctgag aactcatgta cacactttcc agggaatctg     840

cccaacatgc ttagggatct ccgggacgct ttctctaggg tcaagacctt cttccagatg     900

aaagaccaac tggacaatct gctgttaaag gaaagtttgc tcgaagattt taagggctat     960

ctcgggtgcc aggccctttc cgaaatgatt cagttttacc tggaggaagt aatgcctcaa    1020

gctgaaaacc aggaccccga tatcaaggcg catgtgaatt cattgggcga gaacctgaaa    1080

accctccgac tcagactccg gcgttgccac aggttcctgc catgcgagaa caaatcaaag    1140

gccgtcgagc aggtgaagaa cgcattcaat aagctgcagg agaaaggaat ctacaaagct    1200

atgtcggagt tcgacatctt catcaactat atcgaggctt acatgactat gaaaatccgt    1260

aat                                                                  1263


